LOCUS       DAWHXS010000001        46818 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWHXS010000001 DAWHXS010000000
VERSION     DAWHXS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 46818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46818
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.001"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            112..1296
                     /gene="coaBC"
                     /locus_tag="WIE22_000001"
     CDS             112..1296
                     /gene="coaBC"
                     /locus_tag="WIE22_000001"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ4126164.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1363..3519)
                     /locus_tag="WIE22_000002"
     CDS             complement(1363..3519)
                     /locus_tag="WIE22_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ4126165.1"
                     /translation="MPVPQPTAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGENLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGKHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3612..3818)
                     /locus_tag="WIE22_000003"
     CDS             complement(3612..3818)
                     /locus_tag="WIE22_000003"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ4126166.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3877..4494)
                     /gene="gmk"
                     /locus_tag="WIE22_000004"
     CDS             complement(3877..4494)
                     /gene="gmk"
                     /locus_tag="WIE22_000004"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ4126167.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4622..5221
                     /locus_tag="WIE22_000005"
     CDS             4622..5221
                     /locus_tag="WIE22_000005"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ4126168.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5267..6055)
                     /locus_tag="WIE22_000006"
     CDS             complement(5267..6055)
                     /locus_tag="WIE22_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ4126169.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVGALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6381..7736
                     /gene="yegQ"
                     /locus_tag="WIE22_000007"
     CDS             6381..7736
                     /gene="yegQ"
                     /locus_tag="WIE22_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ4126170.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRSIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDAEGWATVEVKNR
                     FAIGDSLEIIHPSGNQTIKLEQMARKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            7870..8346
                     /locus_tag="WIE22_000008"
     CDS             7870..8346
                     /locus_tag="WIE22_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ4126171.1"
                     /translation="MLAACQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKSDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8676..8873)
                     /locus_tag="WIE22_000009"
                     /pseudo
     CDS             complement(8676..8873)
                     /locus_tag="WIE22_000009"
                     /inference="COORDINATES: protein motif:HMM:NF013466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(8877..9485)
                     /locus_tag="WIE22_000010"
     CDS             complement(8877..9485)
                     /locus_tag="WIE22_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4126172.1"
                     /translation="MSNSSFKLSALAIACGLALSACSSGGGDAPSVKPTPTVPGASNP
                     APANPEPSAPAASPLKTGELGEKYFYGEGEITKLVINGETINLTVADEQLSRRVKLWT
                     DFDKIITSQGFENLIYGAAKLADGSYALFVQGKLSDSLPGDTVTYSGDVLHFRSRNLT
                     EGNWFNEGNSFRAAGSFTATVNFAGKSLNGKINSGDGLYMGT"
     gene            complement(9679..10308)
                     /locus_tag="WIE22_000011"
     CDS             complement(9679..10308)
                     /locus_tag="WIE22_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ4126173.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLELQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGGKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            10770..12104
                     /locus_tag="WIE22_000012"
     CDS             10770..12104
                     /locus_tag="WIE22_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ4126174.1"
                     /translation="MPPLIFATFPMKPFAENIPHSLRGSCRDEALPPHTVDCPECGCR
                     ADVPRLDRGEAAFCPRCGHKLFRVGRHPFSGPPAYAAASLILMAFAYGMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGDNAVQTVTEDKICCSRCLYFRDKDEQPCGVCGADLYRRRPKSLS
                     ISLAFLVAAFILYFPTNILPIMISSNPTALEINTIFNGIVYMWDDGDRLIAVIIFSAS
                     IMVPVLKIIAMAVLIYSAYFKPVMSADKMSVLYRITESIGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            12097..13758
                     /gene="pqiB"
                     /locus_tag="WIE22_000013"
     CDS             12097..13758
                     /gene="pqiB"
                     /locus_tag="WIE22_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ4126175.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLTELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRQTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            13758..14276
                     /locus_tag="WIE22_000014"
     CDS             13758..14276
                     /locus_tag="WIE22_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ4126176.1"
                     /translation="MRLFPIAAALTLAACGTMQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSARIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGMNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            14280..14831
                     /locus_tag="WIE22_000015"
     CDS             14280..14831
                     /locus_tag="WIE22_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ4126177.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKQQAFQTAFEGFDINTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            14821..15462
                     /locus_tag="WIE22_000016"
     CDS             14821..15462
                     /locus_tag="WIE22_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ4126178.1"
                     /translation="MPSEKPMNRRIFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVLV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(15455..15685)
                     /locus_tag="WIE22_000017"
     CDS             complement(15455..15685)
                     /locus_tag="WIE22_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ4126179.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            15633..15992
                     /locus_tag="WIE22_000018"
     CDS             15633..15992
                     /locus_tag="WIE22_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126180.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            16023..18875
                     /gene="gcvP"
                     /locus_tag="WIE22_000019"
     CDS             16023..18875
                     /gene="gcvP"
                     /locus_tag="WIE22_000019"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ4126181.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKPPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTIDFGSK
                     EKADQVFAAALASGYNLRRVNDTQVAAAFHETSAYEDLVDLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFTDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISLQPNSGAQGE
                     YTGMLAIRRYQESQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHNAAADQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKALGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(19337..20176)
                     /locus_tag="WIE22_000020"
     CDS             complement(19337..20176)
                     /locus_tag="WIE22_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ4126182.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            20461..20536
                     /locus_tag="WIE22_000021"
     tRNA            20461..20536
                     /locus_tag="WIE22_000021"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20494..20496,aa:Arg,seq:ccg)
     gene            complement(20601..21794)
                     /locus_tag="WIE22_000022"
     CDS             complement(20601..21794)
                     /locus_tag="WIE22_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ4126183.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEAELVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFAYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(21815..22903)
                     /gene="trmA"
                     /locus_tag="WIE22_000023"
     CDS             complement(21815..22903)
                     /gene="trmA"
                     /locus_tag="WIE22_000023"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ4126184.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKKLGDAWQQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEQTLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTPHIESSVLLKKKIL"
     gene            23046..24146
                     /gene="aroC"
                     /locus_tag="WIE22_000024"
     CDS             23046..24146
                     /gene="aroC"
                     /locus_tag="WIE22_000024"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ4126185.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFGSVVQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            24238..24642
                     /locus_tag="WIE22_000025"
     CDS             24238..24642
                     /locus_tag="WIE22_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ4126186.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(24716..26701)
                     /gene="parE"
                     /locus_tag="WIE22_000026"
     CDS             complement(24716..26701)
                     /gene="parE"
                     /locus_tag="WIE22_000026"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ4126187.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSILELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARLRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(26768..27292)
                     /locus_tag="WIE22_000027"
     CDS             complement(26768..27292)
                     /locus_tag="WIE22_000027"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ4126188.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            27458..28753
                     /gene="serS"
                     /locus_tag="WIE22_000028"
     CDS             27458..28753
                     /gene="serS"
                     /locus_tag="WIE22_000028"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ4126189.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(28831..29829)
                     /locus_tag="WIE22_000029"
     CDS             complement(28831..29829)
                     /locus_tag="WIE22_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ4126190.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQIGDCGNAVRADG"
     gene            complement(29954..31030)
                     /gene="prfA"
                     /locus_tag="WIE22_000030"
     CDS             complement(29954..31030)
                     /gene="prfA"
                     /locus_tag="WIE22_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ4126191.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(31134..31904)
                     /locus_tag="WIE22_000031"
     CDS             complement(31134..31904)
                     /locus_tag="WIE22_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ4126192.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDFVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(31912..32904)
                     /locus_tag="WIE22_000032"
     CDS             complement(31912..32904)
                     /locus_tag="WIE22_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ4126193.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWSYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TASGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGAEDVHKELVRLKLV"
     gene            complement(32971..33654)
                     /locus_tag="WIE22_000033"
     CDS             complement(32971..33654)
                     /locus_tag="WIE22_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ4126194.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(33843..35177)
                     /gene="glmM"
                     /locus_tag="WIE22_000034"
     CDS             complement(33843..35177)
                     /gene="glmM"
                     /locus_tag="WIE22_000034"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ4126195.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEANYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMENALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(35307..36158)
                     /gene="folP"
                     /locus_tag="WIE22_000035"
     CDS             complement(35307..36158)
                     /gene="folP"
                     /locus_tag="WIE22_000035"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ4126196.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNARTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSSEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVIMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMRGLPENMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMVGELTGETDAAARVHGSVAAAVAAVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(36298..37191)
                     /locus_tag="WIE22_000036"
     CDS             complement(36298..37191)
                     /locus_tag="WIE22_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ4126197.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            37282..39396
                     /locus_tag="WIE22_000037"
     CDS             37282..39396
                     /locus_tag="WIE22_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ4126198.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRILTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTIGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(39461..40939)
                     /gene="ubiD"
                     /locus_tag="WIE22_000038"
     CDS             complement(39461..40939)
                     /gene="ubiD"
                     /locus_tag="WIE22_000038"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ4126199.1"
                     /translation="MKYKDLRDFIVMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     SALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTSYP
                     VSVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVDNTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(40959..41474)
                     /locus_tag="WIE22_000039"
     CDS             complement(40959..41474)
                     /locus_tag="WIE22_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ4126200.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPSYESDTFGLHCNVVTQPDGQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(41481..41981)
                     /locus_tag="WIE22_000040"
     CDS             complement(41481..41981)
                     /locus_tag="WIE22_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126201.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYR
                     KLVLKV"
     gene            complement(42213..42782)
                     /locus_tag="WIE22_000041"
                     /pseudo
     CDS             complement(42213..42782)
                     /locus_tag="WIE22_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            42829..43425
                     /locus_tag="WIE22_000042"
     CDS             42829..43425
                     /locus_tag="WIE22_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4126202.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSSFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
     gene            43370..44506
                     /locus_tag="WIE22_000043"
                     /pseudo
     CDS             43370..44506
                     /locus_tag="WIE22_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            44560..44635
                     /locus_tag="WIE22_000044"
     tRNA            44560..44635
                     /locus_tag="WIE22_000044"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44593..44595,aa:Ile,seq:cat)
     gene            44710..45468
                     /locus_tag="WIE22_000045"
     CDS             44710..45468
                     /locus_tag="WIE22_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ4126203.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            45469..46533
                     /locus_tag="WIE22_000046"
     CDS             45469..46533
                     /locus_tag="WIE22_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ4126204.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT        11156 a        13079 c        11796 g        10787 t
ORIGIN      
        1 gtctgaaact cggggcttca gacggcattt tgtatccaaa cggtatctaa tgtatccaca
       61 ctttgttata gaatggctgc tgttttttct tcgtaattag aaattgtcaa aatgggcaaa
      121 catattcttt taggtgtaac gggcagtatt gcggcgtata agtcttgcga gttggttcgt
      181 ctgctgaaaa aacaggggca ttcggttacg gtggttatga gccgctcggc aactgaattt
      241 gtttctccgc tgacttttca ggctttaagc ggcaatcctg tcctgaccga cacgcacggc
      301 ggcaacggtt caaacggtat ggaacatatc aacctgaccc ggaatgcgga tgtttttctg
      361 attgcgccgg caagtatgaa taccgtggca aaaatctgta acggcgtggc agataaccta
      421 ctgaccagtc tggcagccgc acggaaatgt ccgcttgcca tcgctcccgc gatgaatgtg
      481 gaaatgtggc tcaaccctgc caaccaacgg aatatcgcac aactggtttc agacggcatt
      541 actgtctata tgccgggctt gggcgaacag gcttgcggag aaaatggcat gggaaggatg
      601 ccggaacctg ccgaattgct ggatttgctt ccggacttgt ggacaccgaa aattttaagg
      661 ggcaagaaaa tcttgattac cgcaggtgcg acatttgaag ccattgaccc tgtcaggggc
      721 atcacaaata cctccagcgg aaaaatgggg gtagctttgg cgcgggcgtg ccgtgccgcc
      781 ggtgcggaag tcagcctgat tcacggacag cttcaaaccg cgctgccttt cggcatatcc
      841 gatacggttc aagccgtcag cgctgaagat atgcatcgcg cagtgcatcg tttgattgaa
      901 aaacaagatg cttttatttc tgttgccgcc gtctcagact ataaggttaa aaacaagagt
      961 acgcaaaaat tcaaaaaaga taaaaatgcc aaaccgttat ccatcgaatt ggatgagaac
     1021 cccgatattt tggcttctat tgcctcattg ccgaacccgc cgttctgcat cggttttgcc
     1081 gctgaaacgg agaatgtaat ggcatatgca cgagaaaaac gtattaagaa aaaaataccg
     1141 gtgatagttg ccaatgatgt ttcaatcgcg atgggcaaac cgaccaaccg gattaccatt
     1201 atcggggacg acggggaact atcttttccc gaaacaagta aagatgaagc ggcgatgcag
     1261 attgttgaaa ggcttgccgt atatttgaac aaataagcaa ttgaacggat aaaccataaa
     1321 gcgggttgcc tgttaatcaa aaggcaaccc gttttacctg cttcaacttc tgatgacttt
     1381 gcggatatat ggaatactat gcagattttg aataatctga ttcaattgat tcagattctt
     1441 gactttcaat aagaatttga attcgacaaa accttccgtt cccgactggg atttagacgg
     1501 tgtttcgacc gactcaatgt ccgcaccgga atcggaaatc gcttgcgcca ttaatgccaa
     1561 caggccgtgg ctgtcttccg attggacttg aagcccgaca cggtagttct gcccgttcat
     1621 attttcccag tctgcatcca gctgctgttc gggatcggac ttcaacaacg tcgggcaggt
     1681 atctctgtgg ataatcatgc cttttccctt aaccaacagc aaacggatgg aatcgccggg
     1741 aacagggtgg cagcactctg caaaatgaat atgcccgctt tcctgcccat cgactttaat
     1801 ggaactgagc ctgacctcac taccgaaatg ctttcctgcc aattcggcaa tatgcatagc
     1861 gacataaaca ggcagggtat gccccatacc gacgttgtac agcacttcct caaatgaagt
     1921 ctgtttatcg ttgagatcgg caaggtattt ttctttgatg ccgtctgaaa gcaggacatc
     1981 tttgggcagc aaactggaca gggctttttg taagaggttc tctcccaaaa cgaccgcatc
     2041 gtgccggtta aggtttttaa tatattggcg tatggcactg cgcgccctgc ctgacacggc
     2101 gaaattcaac cacgcgggat tgggtttggc gtgttcggat gtaataattt cgacggaatc
     2161 accggttttg agcttcgtac gcaacggcat catgatattg ttgatacgtg cggcaacggt
     2221 tttgtgcccg atatcggtat gcaccgcata agcaaaatcg acaggcgttg cccctttggg
     2281 caaagttagg atttttcctt ttggcgtaag gatgtagatt tcgttcggaa acaaatcgac
     2341 tttgacgtgt tcgagaaact caatggcatt ggcactgctt gcctgcaaat ctaagatatt
     2401 tttcagccac cggtttgtgt gaagcaccgc ctgatcgacc gtcttagaat atgatttata
     2461 gctccaatgt ccggcgattc caccttcggc aacagcatcc atttccttgg tacgtatctg
     2521 aacttcaatc ggcaagccgt aaggaccgac caaagtcgta tgcagacttt gatacccgtt
     2581 gcttttcgga atggcgatat agtctttgaa ccgcccgggc ttgggctgat agagggtgtg
     2641 caatgcgccg agtgcggcat aacaggctgg aatgctgttg acaatgacgc ggaaaccgta
     2701 aatatccata acctcggcaa agcgcagctt tttcgccatc attttctgat ggatgccgta
     2761 caggtttttt tccctgcctt tgattttggc ctctatattc gcgcctacca gccgctggcc
     2821 gaatgcgcgc aagactttgc cgacaacgtc ctgccggttc ttccggctct tgtccatcgc
     2881 tttttttaaa gtctcgtagc ggttgggatg caggttttgg aacgataaat cctgaagctc
     2941 ttgatatgcg ttattcaaac ctatgcggtt ggcaatctgt gcatagattt caagggtttc
     3001 ccttgcaatc cggcggcgtt tgtccgggcg catcgaaccg agtgtccgca tattatgcag
     3061 acggtcggca agtttgacga cgatgacgcg tacatctttg gtcattgcca aaatcagttt
     3121 gcggaaactc tccgcctgat gctctgcatg atcttcaaat ttgagttttt cgagtttgga
     3181 cagcccgtcc accatctcgg caaccgtatt gccgaacacc gccgccattt ccccttttgt
     3241 cacgcccgta tcttccaata cgtcgtgcat cacgcctgca caaagaccct gtatgtccat
     3301 atgccaaagg gcgagctgcg tcgcaacggc aatcggatgc gtgatgtagg gctccccgct
     3361 tttgcgggtt tgcccgtcgt gggcgcgaaa cgcataggcg acagcttttt caagctccgc
     3421 ctgttcctcg ggcttgaggt aggaggcggt atggaaaagc agggcacgcg cttcggcggt
     3481 cagggggtcg taaggtgcgg tgggttgagg gacaggcatt tcagacggct ttcggaacat
     3541 attttatttt tcgtttcaaa ccgtcggact gcacggcggc agaatgtgcc gacgtgcgtt
     3601 tccggcagaa tttatttatt gcgcgtcaac agttccgtac cgatatgccc ggcggcgatt
     3661 tcccttaagg cggtaacggt cggtttgtta ttgcggacat cgtccacaag cggcgtattg
     3721 ccattttcaa gctggcgggc ccgacgggcc gctaccaatg tcaggtcaaa atggttggaa
     3781 atttttccgg tacagtcttc ggtggtaata cgtgccatat tatttgcttt ctttcaaaaa
     3841 tatttaaaat aggaaactgg gtattttcgc ggttttttag gaattttcca acaaatctgc
     3901 aataaatccc agttgccgcg accttttcag acggcaggca ttcacaatat ggcgcaaatc
     3961 ctcctccgcc cgcgccaagt catcattgac cacgacaaaa tcgaacaata cggactgttc
     4021 gatttcatgc cttgccttcg acagcctcct ttggataact tcccgactgt ccgtcccgcg
     4081 tccgttcaga cgtgcagcaa gcacatcgaa agacggcggc aggataaaga tgccgacggc
     4141 ttccggcagc gcgttgcgaa cctgcgccgc gccctgaacg tcgatttcca aaattacgtc
     4201 atagcctgcc gccgccaaag cattcacgcc ctccgcgctt gtgccgtaat agttgccgaa
     4261 cacgtctgcg tattccaaaa aagcttcctg cgcgataagc gactcaaact cttctttaga
     4321 aacaaaatga taatgtacgc cgtttacttc gccttcacgc ggcggacgcg tcgtgtgcga
     4381 tacggaaacg cgcaaaccgt tatggtttgc caacagccgc gacaccagcg tggttttgcc
     4441 cgtgccggaa gcggccgaaa tgataaagat gttgcctttt cgataagcgg acatattttt
     4501 tacctgtata ttttccagcc gattgtatca caacggacac tcggtttcct atttgccgat
     4561 gcccatattt tgccgctatt gttttgattg atttggcaag cggcaggctg acggctacaa
     4621 tatggcgtta aaaacatcaa acttggaaca cgcaatgctg gttcatcccg aagctatgag
     4681 tgtcggcgcg cttgccgaca aaatccgcaa aatcgaaaac tggccgcaaa aaggcatctt
     4741 attccacgac atcacgcccg tcctgcaaag cgcggaatac ttccgacttt tggttgattt
     4801 attggtttac cgctatatgg atcagaaaat cgacatcgtt gccggtttgg acgcgcgcgg
     4861 cttcattatc ggcgcggcac tcgcctacca gctcaacgtc ggtttcgtcc ccatccgcaa
     4921 aaaaggcaag ctgccttttg aaaccgtatc gcaaagctac gcgctcgaat acggggaagc
     4981 tgcggtggaa atccacaccg atgccgtcaa acccggttcg cgcgtgctgc tggtcgatga
     5041 tttggttgcc acgggcggca cgatgcttgc cggactggag ctgatccgca aactcggcgg
     5101 ggaaattgtc gaagccgccg ccattttgga atttaccgac cttcaaggcg gcaagaatat
     5161 ccgtgcaagc ggcgcgccct tatttaccct gcttcaaaac gaaggctgta tgaagggctg
     5221 aaaaccgacc ctgccgtctg aaaccggcag ggttgttatt atgcgttcaa atcacgccca
     5281 aatcttgcaa gcccctcaac acaccgtcct catcaacgct ggggcaaaca tatttcgccg
     5341 cctctttcgc cgcctgttcc ccgttgccca ttgccacgcc gaacccgact tctgacagca
     5401 tttccacatc gttcaaaccg tcgccgaacg ccatcacgtc tgccatttcc aaacccaatg
     5461 ccccaaccac gcttctgatg ccgtctgttt tcgacattcc cgcaggcagc agatcgaccg
     5521 cttcctcgtg ccagcgcacc gttttcaagc cttcccgttc cacaatatcc gaccagggcg
     5581 gcatttcgtt ttcctccgca aacaccagca tctgatacac cggtttgctt gaaaaataat
     5641 ccttatcggc aaaaaaatcg ctggcgatat gctgcaaggc gcggcacacg cattccgaca
     5701 gcgcggacac ggcgatcccc tctccgccga caaacgcata atccatgccc aagccatcca
     5761 aatgcgcgca aaccctgccc atcaaaccgg catccatcgg tacttcgcgc acagttttac
     5821 cgtgcagcag cgcaaactgt ccgtttatcg ttaccacggc atccataccc gtttccgcca
     5881 tcatgtccct gaccttttcg ggaatcgtcg ccaaagaccg ccccgttgcc aacgccgtca
     5941 atatgccttt gccgcgcaaa gccgccaccg ccgttttcac ggaaggacgc aaggtatccg
     6001 tatatttgcg gtacagcgta tcgtcaatat cgaaaaacac gattttagga ttcatcacat
     6061 tctctctcgc attcaaactg ccgcattgta tcccaatcag gcaaatactt gataaatcct
     6121 tataaatttc ccggcaaaat tgaccgcgaa cacgaaaagg cggataatcc gcccatcctc
     6181 aaaccctttt cagacggcat ttgcagcaat gccgtctgaa acatttttac aaagcacaca
     6241 aatcatgttt caacacacag gacgacacat aaagcgtcgc cctaatatgt tgccctgatt
     6301 cggaaagggt tacacccctc ccaaataaag tctgattcta ccgccctaaa gggcggggtt
     6361 tcaaccgaaa aggaaacacg atgaaagcac ccgaactctt attgcccgcc ggcggattgg
     6421 aaagaatgcg cgccgcctac gattacggcg cagatgccgt ttacgccggc agcccgcgtt
     6481 actcactgcg cgcccgcaac aacgaatttg ccaaactcga cgttctcgaa caaggcatta
     6541 aagaagcgca cgagcgcaac aaaaaattct tccttaccgt caatacgctg ccgcacaact
     6601 ccaaactcaa aaccttcgtc tccgatatgg agcccttgat tgcgatgaag cccgacgcgc
     6661 tgattatggc ggatccgggt ttgattatga ccgtgcgcga aaaatggccg gaaatgccga
     6721 tccacctgtc cgtacaagcc aacaccacca actactgggg cgtgaaattc tggcagaata
     6781 tcggcgtcga acgcatcatc ctgtcgcgcg aattgagtat ggaagaaatc gccgaaatcc
     6841 gccaacaatg ccccgacatc gaactcgaag tcttcattca cggcgcattg tgcatcgcct
     6901 actccggccg ttgcctactc tccggctact tcaaccaccg cgaccccaac caaggcacct
     6961 gcaccaactc ctgccgttgg gactacaaag tccacaacgc tacggaaagc gatgcgggcg
     7021 atgcccagct tctgcaaggt ttcaactttg aaaaagccca agaagaagcc aaccaaaact
     7081 ttgaaggcat caacggtcaa aaacgccatc cctacgccga caaagttttc ctgattgaag
     7141 agtccaaccg cccgggcgag atgatgccga ttatggaaga cgaacacggc acctacatca
     7201 tgaactccaa agaccttcgc agtattgaag tggttgagaa actcgccaaa atcggcgtgg
     7261 acagcctcaa agtcgaaggc cgtaccaagt cgctctacta cgttgcacgc gtggctcaat
     7321 cctaccgcaa agcgattgac gatgccgttg caggccgtcc gtttgattac agcctgttga
     7381 gcgaactcga aggcctcgcc aaccgcggct acaccagcgg cttcctcgaa cgccaccaaa
     7441 cccaagacta tcaaaactac ctgaccggcc actccatcgc caaacaaagc caatatgtcg
     7501 gccacgttac cgaaatcgac gctgaaggtt gggcaacggt ggaagttaaa aaccgctttg
     7561 ccatcggcga ttcgctcgaa atcatccacc cgagcggcaa ccaaaccatc aaattggaac
     7621 aaatggcccg aaaaggccag cctgtcgatg ttgcacccgg caacggcatc caggtcaaaa
     7681 tccccaatat gcggggcaaa gaaaaagccc tcatcgcacg cgtattgaac ccctgagcca
     7741 caatgccgtc cgaaccccgt tttcagacgg cattttgttg aatcttacgg tacaatggca
     7801 atgatttccc accgcaagca aggatatccg acatgaaaaa aaatcctcat actctccgcc
     7861 gccgccctga tgctggcagc ctgccaatcc ggcagcgcac ctaaaaacgc gcacacggca
     7921 tcccccggca caccggccgg agaatcatgc aaagtcatcc ggggcgacac cgcaggaagc
     7981 ggcaaaaaca tcatttaccg ttgctgcaac ggtcaggcgg caataaatgc agccattgca
     8041 gacggcgtat tggaatcagg gattcccgtc agcttcggcg gcgggggcgt accggttgca
     8101 aacggcagga atctagttac ccgtcaggcg gcaaacggca taggcaaatc cgactcagaa
     8161 gcctacgaac gcgccctaat caatgccgcc cgcaaattcc aacagaccgc aggcaaactc
     8221 ggcggacgcg gcgttaccgg cttccacagc tactttgaca agcaaccgct gcaaggcggg
     8281 caatacgact gtcaggcagg ctcgttccac gtccgcgtcg tcatgcgcgc caatgtcgtc
     8341 cgttaacata tcggcaatca aaaaatgccg tctgaaaccc gttttcagac ggcatttttt
     8401 caggctttac cggcaattcc ttcaacatcc cactccggct gtgactgtaa cactgtagca
     8461 gcaaggtata cggatatata gtggattaac aaaaatcagg acaaggcggc gagccgcaga
     8521 cagtacaaat agtacagcaa gacgagccaa cgctgtactg gtttaaattt aattcactat
     8581 attatcactt tccgtcatcc taaccatata atatcaaaca aaagccccgg tctatccgca
     8641 ccggggcttt cgtcaaatca aggattcaaa cgggattatt gggttttctt accaccaaat
     8701 acgccgaagc tgttatttgt atatgtcttg gcctgttttg gttcatcctt aacatagctg
     8761 tacctgcctg ccatttctgc agccttggca ccatagaatc ctccagagat actacctttc
     8821 tcaccgtttt tccaagtacc ggtaaagcgg ttgccatcaa gacttccctc gaattcctag
     8881 gtacccatat acaatccgtc accactgtta atcttaccgt tcagggattt accggcaaaa
     8941 ttgacagtag cagtaaacga accggctgca cggaatgaat taccttcatt aaaccaatta
     9001 ccttcggtca agttgcgcga acggaaatgc aatacatcgc cgctataagt aaccgtatcg
     9061 ccgggcagac tgtcgctcaa ctttccttgc acaaagagtg cgtatgaacc atctgccaat
     9121 ttagccgcac cataaataag attttcaaaa ccttgggaag taataatctt atcgaagtct
     9181 gtccaaagct tcaccctcct tgacagctgt tcatcagcca ctgtcaaatt aatagtttcg
     9241 ccattgatga ccagcttcgt gatttcacct tccccgtaaa aatatttttc acccaactca
     9301 ccggttttca atggtgaggc agccggtgct gaaggttcgg gattggcagg cgccgggttt
     9361 gatgcacccg gaacagtcgg ggtaggctta acacttggcg cgtctccgcc tcccgaacta
     9421 caagctgaaa gagctagacc gcaggcgatg gcaagtgcac tcaatttaaa tgacgaattc
     9481 gacataacat tctcctaatg acatggataa caaaaataag ttcatcctct tttacgaaaa
     9541 ggcatgatgc ttattcataa atttattgaa aaaaaatttc aagttttcat agtcggttgc
     9601 aaatatgttc cggcaaacag ctgcgtatca tataaagcgc aactccataa aaacaccgat
     9661 acctgatttt tgcattactc aatgcctgtg cggcatcatt ccttccggcg cttcggcatc
     9721 agcggccaag ccaaaggttt cgcgcaacac gactttgtaa aatgcaaagg cttcgcgcgc
     9781 gccttggatg gcttccgctt cggcttcggg agtcaagttc aaagcgttca gatgctcgac
     9841 gaaggcgcgc cagtgtttgc cgcgcccgtc gggatgcgga gcgaggtggc gcgcgccgtg
     9901 ttcgccgttg taatcgagtt tttgcgcgtg cttgaacaaa aacgccgcgc ccaaattgga
     9961 tccttcggcg caatacagcc agccgattgc tttgttgccg gtttcatgcg gcagcggttt
    10021 gccgtattcg taaggtttgc cgcccaaatc tgcaaggtct tgcgttacgg catcgtatcg
    10081 cgccatatat tccagctcgg gaatggcttt gttcaattcg gcatctttat agatgtggtc
    10141 gacggccttg tggaaaacgg attggagttc caaaaattta atgtagtttt ctttgctgac
    10201 aaacggttgg acagacataa cgaggttatc cacgctgtcg tgaactgccg tggtatccgc
    10261 cttcaagcgt ttggcaaatg tcaatgcttg attttcggtt tcactcatat tttttccttt
    10321 atcggtaagg gataaggatg cggtgcgggc aaaatccgca cttgcccgca tatatagtgg
    10381 attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg
    10441 tgctgaagca ccaagtgaat gggttccgta ctatttgtac tgtctgcggc ttcgccgcct
    10501 tgtcctgatt tttgttaatc cactataaaa ataataaaga atcataaacg aaatttatta
    10561 ccacatattt ttggaaaaaa tatcatttgc gtgatgtttt taagcaggta ttttactatt
    10621 ctttacagaa tcgggatttt atcaaatggg ttcggcagtc ggcggacagc cgctcaaaaa
    10681 atatttttgc cggacgacaa gggtttgttc atactgccga acctaccggt tttgcatcct
    10741 gattgggtgc gttgcttttt tcctttataa tgccgccact tatatttgct actttcccga
    10801 tgaagccgtt tgccgaaaac atcccccaca gccttcgcgg cagctgccgc gacgaagccc
    10861 tgccgccgca tacggtagat tgcccggaat gcggctgccg cgcagatgtg ccccggttgg
    10921 acaggggaga ggcggcgttc tgtccccgtt gcggacacaa actcttcagg gtgggcaggc
    10981 atcctttttc cggcccgccc gcttatgcgg cggcttcgct gattttgatg gcgtttgctt
    11041 acggtatgac gtatatcgag gtcgggatac cgggtgcggc atccgtcctt tcgctgcccg
    11101 agatgatgcg cctgatggtg tttcaggatt atggcttttt ggccgaagtg atgtttgtgc
    11161 tgaccttcgg cgcgccggtt ctgttccttt tgctgtgcct gtatgtctat gccgcgctga
    11221 tacggaaacg ggtgtatcct gcgctgcgtt tggcaacgcg cgtaatggtg cgcttgaggc
    11281 aggcgatgat ggtggatgtg ttttttgttt ccactctggt ggcgtatatc aagctctcgt
    11341 ctgtggcaga ggttcgcttc gggccggcgt tttatctgat gttcgcgctg tcggttatgc
    11401 tgattcggac ttcggtatca gttccccagc attgggtgta ttttcaaatc gggcggctga
    11461 cgggggataa tgcggttcag acggtcactg aagacaaaat ctgttgtagc cgctgcctgt
    11521 atttccgcga taaagatgag caaccttgcg gcgtatgcgg tgcggactta taccgtcgcc
    11581 gcccgaaaag cctgagcatc tcgctggcgt ttttagttgc ggcgtttatc ctgtatttcc
    11641 ctaccaacat cctgccgatt atgatttcgt ccaacccgac ggctttggag atcaatacga
    11701 ttttcaacgg cattgtttat atgtgggacg acggcgaccg cttgattgct gtgattatct
    11761 ttagtgccag tattatggtg ccggttttga agattattgc gatggcggtt ttgatttatt
    11821 ccgcatactt caaacccgtg atgagcgcgg acaagatgtc ggttttatat cggatcaccg
    11881 aatccatcgg ccgctggtcg atgattgata tttttgtgat tattattttg atgtgttcgt
    11941 tccacactta tgccgcgcgc gtcattccgg gcagtgcggc agtctatttc tgcctggtcg
    12001 tgattctgac gatgctgtcc gcctattatt tcgacccgcg cctgctttgg gacaaacgcg
    12061 cttcagacgg cattgctttc aatgaaacgg aaaaatatga ctgacaacag ccctcctcca
    12121 aacggacacg ctcaagctcg cgtccgtaaa aacaacacct tcctctccgc cgtctggctg
    12181 gtcccgctga ttgcgctgat tgccggcggc tggctttggg ttaaggaaat ccgcaacagg
    12241 ggacctgtgg ttacgctctt gatggacagc gcggaaggca ttgaggtcaa caatacggtc
    12301 atcaaagtat tgagcatcga tgtcggacgc gttacccgaa tcaaactgcg cgacgaccaa
    12361 aaaggcgtgg aagtaaccgc ccaactcaat gcggacgtat ccggcctcat ccgcagcgat
    12421 acccagtttt gggtggtcaa gccgcgtatc gaccaaagcg gcgtaaccgg tttgggtacg
    12481 ctgctttcgg gttcgtacat cgcctttaca cccggcaaaa gcgacgaggc aaaagacgtg
    12541 ttccaagtgc aggacattcc gcccgttacc gccatcgggc aaagcgggct gcgcttgaat
    12601 ttgattggta aaaacgaccg catcctcaac gtcaacagcc ctgttttgta tgaaaacttt
    12661 atggtcgggc aagtcgaaag cgcgcatttc gacccgtccg accaaagcgt gcattacacc
    12721 atcttcatcc aaagccccaa cgacaaactg attcattccg ccagccgttt ttggctggaa
    12781 agcggcatca atatcgaaac cacaggcagc ggcatcaaac tcaattccgc ccctctgcct
    12841 gccctgctgt cgggcgcgat ttcatttgat tcgccgaaaa ccaaaaacag taaaaacgtc
    12901 aaaagcgagg acagcttcac gctttacgac agccgcagcg aagtcgccaa cctgcctgat
    12961 gaccgttcgc tgtactacac cgcgtttttc aaacaatccg tgcgcggact gaccgtcggt
    13021 tcgcctgtcg agtacaaagg gctgaatgtc ggcgtggttt ccgatgttcc ttatttcgac
    13081 cgcaacgaca gcctgcacct gtttgaaaac ggctggattc ccgtacgcat ccgtattgag
    13141 ccttcccgtt tggaaatcaa tgccgacgaa caaagcaaag aacattggaa acaacaattt
    13201 cagacggcct taaacaaagg cctgaccgcc accatctcca gcaacaacct gctgaccggc
    13261 agcaaaatga ttgagttgaa cgatcagcct tccgcctcgc ccaagctgcg accgcatacc
    13321 gtttatgcag gcgataccgt catcgccaca cggggcggcg gtttggacga cttgcaggtc
    13381 aaactggcgg atttgctgga caagttcgac aaactgcctt tggataagac ggttgccgaa
    13441 ttgaacggtt cgcttaccga gctcaagtcc acactcaaat ctgccaatgc cgccctaagc
    13501 tctatcgaca aactggtcgg caaaccgcaa acgcaaaaca ttccgaacga actgaaccaa
    13561 accctgaaag aattgcgtca aaccctgcaa ggcgtatcgc cgcaatcgcc tatctacggc
    13621 gacgtacaaa atacgctgca aagtttggac aaaaccttaa aagacgttca acccgtcatt
    13681 aacactttga aagaaaaacc caacgcgctg attttcaaca gcagcagcaa agaccctatc
    13741 ccgaaaggaa gccgataatg cgccttttcc ccatcgccgc cgccctgacg cttgccgcct
    13801 gcggtactat gcaaagcaca caatatttcg tgttgcccga cagccgctac atccgccctg
    13861 caacgcaagg cggcgaaacc gccgtcgaag tccgccttgc cgaaccgctc aaacgcggcg
    13921 gactggtcta tcaaaccgac ccctaccgcc tcaacaccgc acaaaaccac gtttgggcag
    13981 acactttgga cgatatgctc gaagcggcgt tgagcaatgc attcaaccgt ttggacagcg
    14041 cacgcatctt tgttcctgcc tcacgcagcg gcagtaccga aaaatggacg gtctatatcg
    14101 acgcattcca aggcagctac acgggcaaaa ccctcatcag cggctacgcc gtcctacccg
    14161 acggtatgaa cagacccttc catatcgaaa ccgaacagca gggtgacggc tacgccgcga
    14221 tgaccgccgc actcgaacag ggactgaaac aggcggcgca acagatggtc gagtaaaacg
    14281 tgaactattg cgaatttgcc gcctcacttc ccgaaaacac cgacaacccg aacaaacatt
    14341 accacgacac gcaatacggt tttccgattg aggacgacaa tgaattgttt gagcggctgg
    14401 tgttggaaat caatcaggca ggattaaact ggacgctgat gctgaagaag cagcaggcgt
    14461 ttcagacagc atttgaaggg ttcgacatca ataccgttgc cgccttcgac gacaccgacc
    14521 gcgaacgcct gcttgccgac gcgggcatcg tccgcaaccg cctgaaaatc gatgccgcca
    14581 ttttcaatgc acggcaaatc caagcgttgc aacaagaata cggctcgttc aagaactggc
    14641 tcgacgcgca ccatccgcga agcaaagacg aatgggtcaa actctttaaa aaacatttca
    14701 aatttgtcgg cggcgaaatc gtcggcgaat ttctgatgag taccggctac ctcaaaggcg
    14761 cgcacgccga aagctgtccg gtttaccgta aaaccctgaa ataccacccg aaatggctcg
    14821 atgccgtctg aaaaaccaat gaacagaaga atcttcctcc tcggtgcagg cgcgttgctg
    14881 cttaccgcct gcggcagaaa atccgcccga acccacgcca aaattcccga aggaagcacc
    14941 gtacttgcct tgggcgattc gcttaccttc ggctacggcg caaaccctgg cgaatcctac
    15001 cccgcgcaac tgcaaaaact gacgggttgg aatattgtca acggcggcgt atcgggcgat
    15061 acatccgccc aagccctgtc gcgcctgccc gcgctgttgg cacgcaaacc caagcttgtg
    15121 cttgtcggca taggcggcaa cgactttctg cgcaaagttc ccgaggagca gacccgcgcc
    15181 aatatcgcga aaatcatcga aaccgtgcag aaggaaaaca tccccgccgt cctcgtcggc
    15241 gtgccgcaca tcacactggg cgcgttgttc ggacatttga gcgaccatcc gctgtatgag
    15301 gatttgtccg aggaatacgg cattccgctg ttcggcggcg cgtgggcgga aattttgggc
    15361 gataataatc tgaaatccga ccaaatccac gccaacggca aaggctatcg gaaatttgcc
    15421 gaagatttga atcaattttt gagaaaacag gggtttagat aaacaaaggt ttatccgcac
    15481 ccaagttgtt tatataatca tgaaccgact gggacaccaa actgcttcgg gacgcatatg
    15541 ccgtctgaag tgcaaagcct acgccataca gccgcatgaa gttgcagcgg tatggcgttt
    15601 tttgaaaaag acggcctgcc ggttcagacg gcatgaccga ccgtccgagc ctgctgtgga
    15661 taaagcccgg acaggctgaa atcatggaat attgcgaacc tgaagaagca tccgacccgt
    15721 acgcaacata caggcgtgcc aacctgatgg cgggtctgcc gctgtttgtc gtgattttgg
    15781 ttctgctcaa tattgttttt ccgcttccgg cgcatccctt agcttggctg gtgcctgcag
    15841 gtttcatggt tttgggcggc ggctttccct tatcgctgcc gcttgtggcg ctgcttgtcc
    15901 tgacctgctg cattctggcg cattgtccgc cattatcccg tcttttgtgc tacccttgcc
    15961 cgaatcatcc gatgtctaaa aattctgcct gatggcagcc ctacaaaccc gaaggagtag
    16021 aaatgaaact gtccgaactg ttcaaccccg acgaatttgc cgcgcggcat ttgagttttg
    16081 gcgacgaagc ggcgttgctt gccgctgtcg gcgagaaaag tatggacgat tttgtcggca
    16141 acaccgtgcc gcaaagcatc cgtatgccgt ctgaactcga tttgcccgat gccctgaccg
    16201 aagcggacgc gttggcaaaa ttgaaaggca ttgcgtcgaa aaacatgatc aacaaatcct
    16261 atatcggttt aggctattac ccgacccgcg tgccgaacgt gattttgcgt aacgtattgg
    16321 aaaatccggg ttggtacacc gcctacacgc cgtatcaggc ggaaatcgcg cagggtcgtt
    16381 tggaagcttt gttgaacttc caacaagtgt gtatcgattt gaccggtttc cctgtggcgg
    16441 gcgcgtcttt gctggacgaa gcgaccgccg ccgccgaagc gatggcgatg gcgcaccgcg
    16501 tgggcaaggt aaaatccgag cgtttctttg tggacgagcg cgtgtatccg caaactttgg
    16561 acgtgatgaa aacccgtgcc aagtatttcg gcttcgagct ggtggtcggc gattttgccc
    16621 aagccgacga aggcgaatac ttcggcgcgc tgttccaata cgtcggcaaa gacggcgacg
    16681 tgcaagactt gcaggacgtt atcggccgtc tgaaagccaa aggcacgatt gtcgccgttt
    16741 ccgccgacat catgagcttg gttttgctga aaccgcctgc cgaattgggt gcggatattg
    16801 cgttgggcaa cacacaacgc ttcggcgtgc cgatgggctt cggcgggccg cacgccgctt
    16861 atttcgcgtt taaagacgag ttcaaacgtt ccgccccggg ccgcatcatc ggcgtatcca
    16921 aagacgcatc gggcaaacct gccttgcgca tggctttgtc cacccgtgag caacacatcc
    16981 gccgcgaaaa agctacatcc aatatttgta ccgcgcaggc attgctggcg aatttggcgg
    17041 gtatgtatgc cgtttaccac ggccctgaag gcgtgaaacg cattgccaac cgcattcacg
    17101 cgctggcttc tgcctttgcc gatgcgctgg tttcagacgg cctgaatgtg gttcacaaag
    17161 tctttttcga tactgttacc atcgattttg gcagtaaaga gaaagcagac caagtgtttg
    17221 ccgctgcttt ggcgtcgggt tacaacctgc gccgcgtcaa cgatactcaa gttgcggctg
    17281 cattccatga aacatcggca tacgaagatt tggtcgattt gtaccgcgcg tttaccggca
    17341 aggatacgtt tacatttgcc gatgatgtca aaggccgtct gaacgccgaa ttgctgcgtc
    17401 aggacgacat tctgcaacat cctgtgttca acagttacca caccgaacac gaaatgttgc
    17461 gttatctgaa aaaactcgaa gaccgcgact tggcgatgaa ccgcagtatg atttcattgg
    17521 gcagctgtac tatgaaactc aacgcgactg cggaaatgtt gccgattact tgggccgagt
    17581 tcaccgacat ccatccttac gctcccgaag cgcaaaccgc cggctaccgc gaattgctcg
    17641 ccgatatgga aaacagcctg aaagccatca ccggctttga cgcgatttcc ctgcaaccaa
    17701 attccggcgc acaaggcgaa tacaccggta tgctcgccat ccgccgctat caggaatccc
    17761 aaggcgaagc gcaccgcaac atctgtctga ttccaaaatc agcccacggt accaaccccg
    17821 ccaccgccgc catgctcggt ttgaaagtcg tcgtcgtcga caccgacgaa cacggcaacg
    17881 tcaacattga cgatttgaaa gccaaagccg agcaacaccg cgacgctttg tctgccatca
    17941 tgattaccta tccgtccacc cacggcgtgt acgaagaagg catccgcgac atctgccgca
    18001 ttattcacga aaacggcgga caggtttaca tggacggtgc gaacctcaac gcccaaatcg
    18061 gcatcatgca gcctgccgaa gtcggtgcgg atgtgttgca catgaacctg cacaaaacct
    18121 tctgtatccc tcacggcggc ggcggcccgg gcatgggtcc gattggcttg aaagcccatt
    18181 tggctccgtt tgccccgggc cataccttga ccgacaccca caacgcggct gccgatcaaa
    18241 ccgccgtggc tgccgcagca tatggttctg catccatcct gccgattact tggatgtacc
    18301 tgaccatgat gggcaaacaa ggcatggaac aggcaacgcg ctgggcattg ctcaacgcca
    18361 actacgtcgc caaagccttg ggcgaggatt atccgattct ctacaccggc aaaaacggcc
    18421 gcgtcgcgca cgaatgtatc gtcgacttgc gtccgctcaa agccgaaagc ggcattaccg
    18481 aaaccgacat cgccaaacgc ctgatggact acggcttcca cgccccgacc gtctccttcc
    18541 ccgttgccgg cacgctgatg atcgaaccga ccgaaagcga gagcaaagcc gaactcgacc
    18601 gcttcatcgc cgccctgaaa caaatcaaac aggaagtgct gaaagtcggg cgcggcgaat
    18661 ggccgaaaga cgacaaccca ctggtcaacg cgccgcacac cgccgcagat ataaccggca
    18721 actgggcgca tccgtattcc cgcgaagaag ccgtcttccc gttgccgttc gtccgcgaac
    18781 acaagttttg gcccttcgtc aaccgcgtgg acgacgtgta cggcgaccgc aatctcgtgt
    18841 gcagctgccc accgatggaa aattatgaag actgactgtt gatatcttaa aaaatgccgt
    18901 ctgaaacatt ttcagacggc attttcatca acggcaaacc agttgcacca atacacgtat
    18961 ctcgactata actttaaaac aaatgagtta aaccagtatc catacatcag cttttttatc
    19021 atcctacttt ttattcatcc gatcgtgcaa acagatttca aagatgaaaa gcctattcac
    19081 accctttgat gtcatttcca cacggacaaa caaatatagt ggattaacaa aaaccagtac
    19141 agcgttgcct cgccttagct caaagagaac gattctctaa ggtgctcaag caccaagtga
    19201 atcggttccg tactatttgt actgtctacg gcttcgtcgc cttgtcctga tttttgttaa
    19261 ttcactataa attcccataa aaaaacggag cagatacctg ccccgttttt atttaatccg
    19321 aaattttaat ctaaatttag aattttgcac cggattggtt tgccatatag tcaacagccg
    19381 ctttgacttc gtcatcgctc aaacctgcat tgccgccttt ggcaggcatc gcgttaaagc
    19441 cttcaagggc gtgtttgtgc aaggtttctt tgcctttttt gatacgcggt gcccaatcgt
    19501 cttttttgcc tatgccggga ataccgggaa tcgaaccgcc gtggcacacc tgacaggttg
    19561 cttcgaagac ttttttaccg tcaacgccga ccgcaggggc tgccgcaccc ttgtcttctg
    19621 ccttcgcttc tgccggagct gcactatcgg caggagcaga agctgttcct gaagcggcat
    19681 tgtcggcagg cgcagcctca tcaggattcg ggaaagaacc gccgcttttg ttcgccatgt
    19741 aagtaatcgc ccgtttaagt tcctgatcgg tcaggtctgc cgcaccgcct tttgcaggca
    19801 tggcgttaaa gccgttcagc gcgtgttgga acaaggtatc gaagccttgc gcgatacgcg
    19861 gtgcccaatc gccgttgtgt tccagtttcg gagcgttcgg cacattgctg tccgccgcgt
    19921 ggcattggat acagattttg ccgaaaatct gttcgccttg gcgttcgccg acggggatgc
    19981 cgtcgcccat cgtcaattgt ccgacaggct ggatacgggt ctgcgttgct gcttccgtag
    20041 tggcatcgac atcgccgaac gagccgctgc ccgccagctt aatcaggaaa taaaggactg
    20101 caataacaat aacgataccg ctcacaaggg taaacagtgc agagccttgg gctttgttgt
    20161 cgcggagttg tttcatttgg taggcctcgc cgtcaggtta ggttgtgctg taaattatag
    20221 tttggtgtgt taaacgcagt taacaatatt ttgctggatt atactgaatt cacagggtct
    20281 ttccaatcgc tatcattgaa aatatgaaaa aatttgccaa cggtatctgt ataaaacaaa
    20341 taatcctttg aaaataattg tttatcctca agaaaactct ccttatgccg ccatacgccg
    20401 cctgccggcg caagataacc tttgccaatt tgcagaattt acgttaacct tacgttttac
    20461 gcacccatag ctcagttgga agagtgtcag tttccgaagc tggaggtcac aggttcgatc
    20521 cctgttgggt gcgccaatta taaagagacc gtctgaaaga taaatatttt tcagacggtc
    20581 ttttgactta cttcaaactc ttatttcaag acttccgcaa atgcgcgggc aacatagtcg
    20641 gtattcgacg tattcagtcc ggcgacgcac atcctgccgg aatccagcag gtaaacggca
    20701 aattcgtcgc gcagcctgcg gacttgttcc acgctcaatc ctgtgtagcc gaacatgccg
    20761 cgctgtttga tgaaataagc gaaatcgcga tcggggattt gcgcagttaa gacatcataa
    20821 agtttctgcc gcatcgcacg gatgcggtcg cgcatcatat aaacctcgtt ttgccacaag
    20881 gcgtaaagtt cggggctgtt catcacgtcg gcggcgatat acgcgccgtg cgcgggcggg
    20941 ctggagtaga tacggcggac agtgaatttg agctgtccga ataccaattc cgcttcttcc
    21001 ttattcgggc aaaccacgct caggccgccg acgcgctcgc cgtagagcga caggtttttc
    21061 gagaaggaat tgctgacgaa cagcggcaat tccatttcca ccgctttgcg gacggcgtag
    21121 gcatcgctgt ccaaatcgcc gccgaatcct tggtaggcaa tgtccataaa cggaatcagt
    21181 ttgcgcgttt tgatgatgtg caacacttcg tcccattgtt gttccgacat atccacgccg
    21241 gtcgggttgt ggcagcaggg atggaggatc aggacgctgt tttcgggcag ggtgttgaaa
    21301 aacgcggtca tttcgtcgaa tttcacgccg acagtggcag ggtcgtaata tgggtaagtg
    21361 ccaacctcga aacctgcacc ttcaaaaatg ccgcgatggt tgtcccaagt cgggtcgctg
    21421 acgtaggcgc gcgcttcagg aaaccagcgg tgcaggaaat ctgctccgac tttgagcgcg
    21481 cccgagccgc ccaaggtttg cacggtagcg atgcgtcctt gcgcgagcgc agggttgtct
    21541 ttgccgaaca ataaatgctg caccgcgctg cggtaagtgt ccaagccttc catcggcagg
    21601 tagggcgacg gcgcaggcgc ggcggcgcgg gcggtttcgg ctcggctcac ggattccaac
    21661 acgggcattt tgccttcgtc gtcgaaataa atgcctatgc tcaaattgac tttttcaggg
    21721 cgcgggtcgt ttttgaaggt ttcgaccaaa ctcaaaatcg gatcgccggg atagtattcg
    21781 atgtgtcggt acatagttct tacctcttga tttttcaaag tattttcttt ttcaacagta
    21841 cgctgctttc aatatgcggc gtaaacggaa attggtcgaa cagggcggca cgttcgaccg
    21901 catgggtttc caccaaggtg tccaaattag cacgcagcgt ttcgggattg caggaaatgt
    21961 agatgatgtt gtcaaactgc gacaccagct tcaaagtttg ctcatcgatg ccggcacgcg
    22021 gcggatcgac gaaaatagtg gaaaatgcgt aatccgtcaa agcaataccg ccatccttaa
    22081 ggcgtttaaa ctcacgtttt ctggtatagg cttcggtaaa ttcttcagca gacagacggg
    22141 cgattttaat gttgccgatg cggttggctt cgatattcca ttgcgccgcg ctgacggagg
    22201 ttttggagat ttcggttgcc aaaacacgtt caaacttttc agataaagga agcgtaaagt
    22261 tgccgtttcc gcaatacagt tcgagcaaat ccccatccaa tccttctgct gcaccgcagg
    22321 cccattcaag cattttctga cacacggcgg cattcggttg ggtaaaactg ccttcaattt
    22381 gccgataacg gaaatcccgg ttgccgacct tcaaagtttc cgttacatag tcctgtttta
    22441 agactatttt ctgtcccctg ctccgcccaa taacggaaat atccaactgt tgctgtaacg
    22501 cttgcgccgc ctgttgccat gcgtcgccga gttttttgtg gtaaatcatg gtaaccagca
    22561 tttccccgct gagcgtggac agaaattcga cggcatacca acggtttctc agttcggcag
    22621 attgggcggc aacggcaatc aattcaggca tcaggcaatt gactgcctct gaagcagcat
    22681 cgaagcggtc gcaacggatc aggcttgccc cgccagcctt ctgccctttt tcaaacatgg
    22741 cgtaaaacat ttcaccgcct tcgtgccaaa tacggaactc ggcacgcata cggtaatgtt
    22801 tgtccggaga ttcatacact tcccactcag gaacatccaa acctgcaaaa agggttttaa
    22861 ggtggtcttt tttatcttga agctgctgcg tgtagtcgct catatcgcta tcctgaaaaa
    22921 tcagaccgga ttataagccg attcactgca aaacacgcaa cccctttacc ggcatataac
    22981 ccgttttcct ccaactttta tgctaaaatc cgccgtctcc aacataccgc cgacagaagg
    23041 aaaatatggc aggcaacact ttcggacaac tcttcaccgt gaccacgttc ggcgaaagcc
    23101 acggcgcagg tttgggctgc atcatcgacg gctgcccgcc cggcttggaa ttaagcgaag
    23161 cggatatcca atttgacctc gaccggcgca aacccggcac cagccgccac gttacccaac
    23221 gccgcgaagc cgaccaagtc gaaatcctct ccggcgtatt cgaaggcaaa accaccggca
    23281 cacccatcgc cctcttaatc cgcaataccg accagcgcag caaagactat ggcaacatcg
    23341 ccaccagctt ccgccccggc cacgccgact atacctattg gcacaaatac ggcacgcgcg
    23401 actaccgggg cggcggcagg agttccgccc gcgaaaccgc cgcccgcgtt gctgccggag
    23461 ccgttgccaa aaaatggttg aaagaaaaat tcggcacgga aatcaccgcc tacgtgaccc
    23521 aagtcggcga aaaagaaatc cggtttgaag gctgcgaaca catttcccaa aatccttttt
    23581 ttgccgccaa ccatagccaa attgccgagc tggaaaacta tatggacagc gtgcgcaaat
    23641 ccttggattc cgtcggcgcg aagctgcata tcgaagccgc caacgtccct gtcggcttgg
    23701 gcgaacctgt ttttgaccgc ctcgatgccg aaatcgccta cgcgatgatg ggcatcaacg
    23761 ccgtcaaagg cgtggaaatc ggcgcaggtt ttggcagcgt tgtccaacgc ggcagcgaac
    23821 acggcgacga actgaccccg caaggcttcc tgtccaacca ctcaggcggc atcctcggcg
    23881 gcatcagcac cggacaagac atccacgtca atatcgccat caaacccacc agctccatcg
    23941 ccaccccgcg ccgcagtatc gacatcaacg gcaaccccat cgaactcgcc acgcacggca
    24001 ggcacgaccc ctgcgtcggc ttgcgcgccg cgccgattgc agaagctatg ctggcattgg
    24061 ttttaatcga ccacgccctg cgccatcgcg cgcaaaatgc ggatgttcag gttaatacgc
    24121 ccgacattac cctttcaaac aaataaaaat ttagccaaaa cacagacttt ataatagaat
    24181 accgagcatt gccgtgccgc tttgacgcac gggtttgttt agaggaatac aaccgaaatg
    24241 acacaagaaa ccgctttggg cgcggcactg aaatccgccg tccaaactat gagcaaaaag
    24301 aaacagaccg aaatgatcgc cgaccacatc tacggcaaat acgatgtatt caaacgcttc
    24361 aaaccgttgg cgctcggcat tgatcaggat ttgattgccg ctttgccgca atacgattcc
    24421 gcactgattg cacgcgtcct cgccaaccac tgccgccgtc cgcgttatct gaaagccttg
    24481 gcgcgcggcg gcaaacgttt cgatttgaac aaccgtttca aaggcgaagt tacccccgaa
    24541 gaacaggcga tcgcgcaaaa ccatcctttc gtgcagcagg ctttgcaaca gcagtccgcc
    24601 caagctgccg ccgaagcagc cggatcttcc gcagcagaat aaatccccaa acgaaatgcc
    24661 gtctgaaaac cgatttggtt tcagacggca ttttttcgta tgcggcaatc acggttcaaa
    24721 tatccaattc cgccgtatcg ccttcgcgtt ccatccaagc gcggcgggcg gcggcttcgc
    24781 ctttgcccat cagtttgacg aagatgtcgc gcgtctcgtc atctgcacct tctgggattt
    24841 gtacctgcaa caggcggcgg gtgtcagggt gcatggtggt gtctttgagc tggtcggggt
    24901 tcatttcgcc caagcccttg aaacggctga tggaataggc ggtttctttg acgccttctt
    24961 tttgcagccg ctccaaaatg ctgtcgagtt cgttttggtc gagggcgtag aatttgcggg
    25021 caggtttgct tttgccttgt gcgttgacat cgacgcggaa cagcggcggc tgggcgacgt
    25081 agatgtgtcc gtcggcaacc agtttcggga agtggcggta gaataaggtc agcagcaaaa
    25141 cttgaatatg cgagccgtcc acgtcggcat cggacaggat ggcgattttg ccgtaacgca
    25201 ggccgcttaa atcgggatgg tcgttgatgc cgtgcggatc gacgccgatg gcaactgaaa
    25261 tgtcgtggat ttcggcgttg ccgaagagct ggtcggggtg gacttcaaaa ctgttgagta
    25321 ctttgccgcg caggggaagg atggcttggg tggctttgtc gcgggcgagt ttggcagagc
    25381 cgccggcgga atcgccttcg acgaggaaga gttcgttttc gcggatgtct tcgctttcgc
    25441 agtcggtcag tttgccgggc aggacggcga caccgctgcc ttttttcttt tcaatctttt
    25501 taaccgaacg cagccgcgcc tgtgcctgac ggatggcgag ttcggcgatt tttttgccaa
    25561 agtccacgtt ttggttcagc cacaattcca aagggtcacc cgatacggtg gcaacgagtt
    25621 tcagcgcatc gcggttggtc agtttgtctt tggtctgacc ttggaactgc gggtcgagga
    25681 cacgggcaga gaggacaaag gcagttttgc cgaacacgtc gtcgctttgc actttcacgc
    25741 cgcgcggtaa gaggttgtgc aggttgatga agttgctgac ggcgttgaac acggcttgtt
    25801 tcaagcccgc ttcgtgcgtg ccgcccagcg gggtggggat gaggttgaca tagctttcgt
    25861 tggcacacga gccttcttcc agccaagtca gggcaaatgc tgcgccttcg ccgatgctga
    25921 aatcgccgtt gtgttcgtct gaaatgtagt tttcgcaaga gaacagcggt acggcttcct
    25981 gcgcgtcggc aatcaggtcg gtcagatagc ttttcaggcc gtcggggtaa tgccaggttt
    26041 gggtgtgcgc ttcgtcttcg cctttgaccg gacgggtcag ggaaacgcgc acacccggca
    26101 gcagcacggc tttggcacgc agcaggcgtt cgagttcgag aatgctgtaa ttcgggcttt
    26161 caaaatattt gccgtccggc cacacgcgca cttcggtgcc gctgtctttg acagcgcatt
    26221 tgcccacttg tgccaacggt tcgaccacgt cgccgccggc aaacacgata cggtggattt
    26281 tgccttcgcg tttgaccgtt acctcaaggc gggtggaaag ggcgttggtc acggatacgc
    26341 ccacgccgtg caggccgcct gaaaaggcat acgcgctgcc gccgtctttt ttgttgaact
    26401 tgccgcctgc gtgcagacgg gtgaacacga gttcgactac gggtacgcct tcttcagggt
    26461 gcagaccgac gggaataccg cggccgttat cacggacgga aagcgaaccg tcttcatgaa
    26521 tttgcacgtc gattgccgta gcgaaaccgc ccaacgcctc gtccgccgcg ttatcaatca
    26581 cttcctgaca gatatgggtc gggctgtcgg tgcgggtgta cataccggga cgttctttga
    26641 ccggttccaa acctttgagg acggtgatgc tggattcgct gtattggttg tttttagcca
    26701 taggaataat ctgaaagtaa gaaaaacaac gctttcagac ggcctgaaag cgttgcgttc
    26761 cgttgtttta ccggttgccg gaaggttggc gggcggcaaa gtcttcataa ctttccatac
    26821 cgcgcaggaa gcgggaagag agttctttca acgcccccaa ataaacgtcg cgtttgaaat
    26881 caatcacttc gtcaacgggc gcccaatatt gatgccaacg ccagccgtca aattcggggt
    26941 ggcgggtggc gcgcaggttg acatcgcaat ctcggccggt caggcgcagg agataccaaa
    27001 tctgcttctg cccgcgatag gatccgcgcc attcgcggcg tacccagttg ttcggcacgt
    27061 cataacgcag ccagtcgcgc gtgcggccga taattttgac gtgttgcggc aaaagcccga
    27121 cttcttcgta caactcgcgg tacatggcgg tttcggggct ttcgcccggc ttgatgccgc
    27181 cttgcggaaa ctgccaagaa tgttcgcgca cgcgcttacc ccaaaagact tcgtcgcggt
    27241 tgttggttaa gatgataccg acattggggc gatagccttc tctgtccaac acggtgtcgc
    27301 cctccgttaa attcaatctt gggattttcc cacaaatcga acggttttga caaatcagac
    27361 ggtatggttc aagcatgtcg aaacacggac ggattcggga aaatatctta aatttggttt
    27421 acaataatgt gtttcaaatt aattcgggaa tcagaccatg ttagatatcc aattgctccg
    27481 cagcaacacc gccgccgttg ccgaacggct tgcacggcgc ggttatgact ttgataccgc
    27541 acgttttgac gcactggaag aacgacgcaa gtccgttcag gtgaaaactg aagaactaca
    27601 agcctcgcgc aacagcattt ccaaacaaat cggcgcactg aaaggtcagg gcaaacacga
    27661 agaagcgcag gcggcgatgg atcaagtcgc ccaaatcaaa accgatttgg aacaggctgc
    27721 cgccgatttg gatgccgttc aaaaagaatt ggacgcatgg ttgttgagca tacctaacct
    27781 gccgcacgaa agcgtacctg ccggtaaaga cgaaaccgaa aacgtcgaag tccgcaaagt
    27841 cggcaccccg cgcgaatttg actttgaaat caaagaccat gtcgatttgg gcgaaccttt
    27901 gggtttggat ttcgaaggtg gcgcgaaact ttccggcgca cgatttaccg tgatgcgcgg
    27961 acaaatcgcc cgcctgcacc gtgcgctggc gcaattcatg ctcgatacgc acacgctgaa
    28021 acacggctac accgagcatt acacacccta tatcgtggac gacaccacct tgcaaggcac
    28081 gggtcagctg ccgaagtttg cagaagattt gttccacgtt acccgtggcg gcgacgagag
    28141 caaaaccacg caatatctga ttccgacagc tgaagttacc ctgaccaata ccgttgccgg
    28201 cagcattatc ccgtccgaac aactgccgct gaagctgacc gcgcattcgc cctgtttccg
    28261 cagcgaggcg ggttcgtacg gcaaagacac gcgcggtctg attcgccagc accagttcga
    28321 caaagtggaa atggttcaaa tcgttcatcc cgaaaaatca tacgaaacgc tggaagaaat
    28381 ggtcggccat gccgaaaaca tcctgaaggc tttggaactg ccctaccgcg tgattaccct
    28441 gtgtaccggc gacatgggct tcggcgcaac caaaacgtat gatttggaag tttgggtgcc
    28501 ggcgcaaaat acctaccgcg aaatctcaag ctgctccaac tgcgaagatt tccaagcccg
    28561 ccgcatgaag gcgcgtttca aagacgaaaa cggcaaaaac cgcttggtac atactttgaa
    28621 cggctccggc ttggctgtcg gcagaacgct ggtcgccgta ttggaaaacc atcaaaacgc
    28681 cgacggcagc atcaacatcc ctgccgcact gcaaccgtat atgggcggcg ttgccaagtt
    28741 ggaagtgaaa taagtttgca agctgcctga acgtcaaatg ccgtctgaaa cctggttcag
    28801 acagcatttc ctttaaactt ttaaaacacg tcagccgtcg gcacgaaccg cattgccgca
    28861 atcgccgatc tgtccgacct cgcggatatt ggacagcgta acttccgaaa tattacccaa
    28921 cgcctcttcc gtcaaaaacg cctgatgacc ggtaaacagc acgttatggc aggacgacaa
    28981 ccggcggaac acgtcgtcgg taatcacatc gttggatttg tcttcaaaaa acagctcgcg
    29041 ctcgttctcg tacacatcca tgcccaatgc gccgattttc cggcgtttca acgcctcaat
    29101 cgcggcggca ctgtcaatca gcccgccccg gctggtgttg ataatcatca cgccgtcttt
    29161 cattttgtcg aacgccgctt cgttcagcat atagtggttt tccggcgtgg cggggcaatg
    29221 cagcgtgatg atgtccgact gggcatacag ctcgtccaaa tccacatacc tgccgccgat
    29281 tttttccgct tcggggttgc aaaacggatc gtgagccagc aggttcatgc cgaaaccctt
    29341 taaaatccgc atggttgcaa taccgatttt ccccgtgccg ataacgcccg ccgttttgcc
    29401 gtacatattg aaaccggtca gaccttccag cgaaaaattc gcatcgcggg tacgctgata
    29461 ggctttgtgg atacggcggt tcaaagtcag catcagaccg accgtatgtt ccgcaaccga
    29521 ttcgggcgaa taggcaggca cgcgcacgac tttcaagccc aactcttcag ccgcctttaa
    29581 atccacatta ttgaagccgg cacaacgcaa cgccacagtt ttcacgccaa tttgcgccaa
    29641 tttttccaac acgggccggc tgccgtcgtc gtttacaaaa atacaaaccg cttccgcgcc
    29701 ttccgccatt ttcgccgttt tcgcatccag cataaaatca aaaaactcca gctcgaagcc
    29761 gaaatgccgg ttggcgcggg taaaatgttc gcggtcatag cttttcgtac cgtaaatcgc
    29821 aatcttcatc aatatgtcca gttgtcgtct atggttgaga aacggcataa tacactgaat
    29881 tcaaacaaat cagtagaata tggtggatta aaattgattg catgcacggc atttccattt
    29941 caaaacacaa aactcaatcg cccattgccg ccaaaagctc cgcctgatgt tcggcaatca
    30001 gggcatcggt gatttctgcc aaatcgccgt ccatcacaaa atccagcttg tgcagggtga
    30061 ggttgatgcg gtggtctgtt accctgcctt ggggatagtt gtaggtgcgg atgcgttcgc
    30121 tgcggtcgcc gctgccgatg agggatttgc gttcggcggc ttctttggct tgggcttcgc
    30181 gtttttgcgc gtcgttcagg cgggcggcga gaactttcat tgcctgcgct ttgttggcgt
    30241 gttggctgcg gccgtcttgg cattcgacca ccatgccggt gggcaggtgg gtaatgcgga
    30301 cggcggaatc ggttttgttg atgtgctgac cgcctgcgcc ggatgcgcgg aaggtgtcga
    30361 tgcgaaggtc gacggggttc aactcgatgt cttccagttc gtccgcttcg ggcatgacgg
    30421 caacggtgca ggcggaggtg tggatgcggc cttggctttc ggtggcgggg acgcgctgca
    30481 cgcggtgtcc gcccgattca aatttcaaac ggctgtacgc gcctaagccg ataatacggg
    30541 cgataacttc tttatagccg cccaactcgc tttcgttggc ggacacgatt tcaacctgcc
    30601 agcggttgcg ttcggcgtag cggctgtaca tacgcagcaa atcgccagca aacagcgcgg
    30661 cttcgtcgcc gcccgttccg gcgcgtactt cgataaagat gtttttgtcg tcgtcggcat
    30721 ctttgggcag cagcagtttt tgcaattcgg tatcgagtgc gccgattttg gctttcgccg
    30781 cttcgatttc ttcggcggca aagtctttca tttcggggtc ggacaacatt tcttcggcat
    30841 ccgccaaatc gctttgcgcc agttgatagt tttggaatac ttcgacgacg ggggtcagtt
    30901 cagcgtgttc gcgcgtgagc ttgcggtagt tgtccatatc ggatgtggct tcgggttgcc
    30961 ctaaaaggtg ggtaacttct tccaaccggt cgccgagttg ttgcagtttt tctaagatag
    31021 acggcttcat aattcttcca taacaaacgc cgcctgaatt tcagacggca tcaacactga
    31081 attattataa taggttttcc ggacattcaa aaagataatc tcagatggat aacctaccgt
    31141 cccaacaggg catcggcatt gcgctccgtt accttggcaa tctcttccac acaggttccg
    31201 cggatttccg cagcaatctt tgcaataccc ggaatattgg caggcgtatt aatctctttt
    31261 ttcagcataa acgggctgtc ggtttccaat acgaaatccc cgtcgttcaa ggctttaagc
    31321 gtatcgcgca ctttacgcgc gttcggattg agcaacaacg aaccgatgcc gattttgaaa
    31381 cccagtttcg tcaacacgcg cgcttcttcc gcgctgcctg agaaggcgtg aacgatgccg
    31441 ccttgggcaa agcctgtctg tttgacggcg gcggcgatgt cggcggtggc tttgagatta
    31501 tggataatca cgcggcggcg cagggtttgc gcgatggcaa gctggcggac gaaaacttgg
    31561 atttgccgtt cgcgctgcgg cggcgtttgg gttttgtcgt aaaaatccaa accgatttcg
    31621 ccgacccatg cctgcggata acgtgccaac atcgcttcca ggcggacgaa atcacgctcg
    31681 gcaatgccgt ctgaaaacca aggatggatg ccgagcgcaa tacggatttg accgtgttcg
    31741 gacggcattt ccgacagttc cgccacgctt tgccaatcct gcggacaggt ggcgggaacg
    31801 ataaaccgct tcaccccaac tttccgcgcg gcggtcaaaa tgtgcggcag gttttcgcgt
    31861 aaggcgggat cggcgaggtg gcagtgggtg tcggtgaagt tcattccggt ttcacactaa
    31921 ctttagtctt actaattctt tgtgaacatc ttccgcgccc catccttcgg aaacggcaag
    31981 cgtcatcaat gcggttgctg tttccaaatt gcatttgccg ccgttgatga tgcctgagtt
    32041 gcggaacgcg ttgccttgcg cgtaaacggc agcggttctg ccttgtcgga cttggctgat
    32101 gttgagcagc agtttgcctt gctgcgtaaa gtcttgcacg gcgcggatga aaccttcgtc
    32161 tgcgggcgtg ttgccgtgtc cgtaactttg caggataagg gcgtgggcgg gaagctgtcc
    32221 gagtccgtcg gcaagttctt ggacggcaaa gccgggaata agcgtgcgga cggcgatttt
    32281 tgcctgcggg tcaggatagc ggattttgag gccgtctgaa acggccgccg catcttggga
    32341 cggaatacga agattgtacc agccttgagt ttcatcccat tcggcgatga caccgaaatg
    32401 cggattatcg aagcctgagg cggtttcggt gctgactttg ctgctgccga cggcgggata
    32461 aagtttgccg tcaaaggcaa taagcgtctg cttcaatccg agtgggaatg cggcaacggc
    32521 agtcgccaga ttgcgcggcg cgtcgctgcc cacggcgttg tacgaccatt gggaaccggt
    32581 caggacgatc ggcttgtcca aaccctgcag cgcgagggcg aacagattgg cggtataggc
    32641 gagcgtgtcc gtaccgtgca gcaccaaaac gccgtcgtac tgcggcagcg cggcggcaat
    32701 gatgtccagc caagcgcacc agttttgcag ttggacggcg gaagaatcaa tcagcgtccc
    32761 acaaacgtgc caatcgaaat ccatgccgtt tgaaaacggc gcgagtgcct gcccgaccag
    32821 cgcggtatcg gggcgcaggc cttcgctgct ttgcgtcatg ccgatggtgc cgccggtata
    32881 aaggacaaaa attttctgtt tcatggctca tcctgaaaat gcaaagaccg cctgaaacgc
    32941 gaaatgcgtc cggacggtcg aaaatctgat ttactgctgc ttctgtgccg cttttttcgc
    33001 tgccttttcc gccttgcgtt tggcgcgttt ttcgctcaat tgtgcgcggt aaagctgata
    33061 atgtcggcgt ttgcgccacc agaaccacgc cagcgccgcc gccagcacgc ccaatgcgat
    33121 gaagatgccg gattgcaggc tgtgcatttt cgccatcagc caatcgatgt tgtgcgcgcc
    33181 gtactcgccc aagtaaatcc aaacgggcac ggaaatcagc gcggcaagcc cgtccataat
    33241 cagaaagcgc agatacgata ctttgcggct gatgccggcg gtaacgaaaa cggcagtccg
    33301 caaacccggc aggaagcggg cgacaaacag aacccagttg ccgtatttgt cgaatttttc
    33361 ctgaacctgt gcgtaacgtt tcggcgtcat gatgcgcgca atcggtttga acttgaggat
    33421 tttctgcccc caaattcgtc cggcggcgaa catgatgccg tccccgacca atacgccgag
    33481 cataccgact gcaaacataa tatgcggatt ggtataaccc atacccgaaa tcacgccgcc
    33541 tgttaccaag gtcaaatcct cggggatcgg cacgccgaaa ccgcagatga ccaaaacgaa
    33601 aaacacggcc gcatagccgt attcgacaaa aaaggcttcc aaaagggcaa acatagcgga
    33661 tattccattg tcggagataa aaagtcagaa caagccgaaa cattttctgc gcgaaacggc
    33721 attctatcaa agattatgcc gtctgaaagc ggcaaggggc agattcgcgc cctctgccgg
    33781 acgttcagac ggcatttaaa caggaaaacc gccgctgttg aaacggtttt gcctgatttt
    33841 gcctaaaggc tgccgatggc ggaagcaata cgttccgcac cgtcgcgcgc ccaatccgcc
    33901 tgccgcgcct ccaccatcac acgcacgacg ggttcggttc ccgaagcgcg caacacgaca
    33961 cgccctttgc cttcgagttc tttttccact tccgccaaca cgtcttttga ggcttcctgc
    34021 cagttttgcc ctttgccgat acggacatta atcatcgttt gcggatacgg ctgccaatcg
    34081 gcgcaaacgg tggcgaggtc ttggttcagc gtttgcagtg ccgccaaaac ttgcagcgcg
    34141 gaaataatgc cgtcgccggt gttgtgtttg tccatgcaca aaatatggcc gctggcttcg
    34201 ccgccgatga gccagccgcg ttggttcaac tgttccaaca catagcggtc gccaactttg
    34261 gcgcggcaga aatcgacgcc ctgctctttc agggcgtttt ccatcgccat attggtcatg
    34321 accgtgccga ccacgccgcc gatgttgata ccttctcggg cgcgggcttt agcgatgacg
    34381 taaatcaggc tgtcgccgtc gtaaacctta ccgtttttat cgaccatcat caagcggtcg
    34441 ccgtcgccgt ctaaggcgat gccgtagttg gcttcatgct gtaaaacggc ggcctggagt
    34501 gtcttggtat aagtcgcacc gcatttttca ttgatattgt agccgttggg ttcgtcgccg
    34561 atgctgacga cttgtgcgcc cagttcgtga aacaccttgg gagcgacacc gtaccccgcg
    34621 ccgttggcgg tatcgataac caacttcaaa ccccgaaggt cggaatggct gggaaaggtg
    34681 gatttgcaaa attcgatata gcggtcgtcc gcaccgctga tgcggcgggc gcgcccgagg
    34741 cgtgcagaag gctgggtttt catttcttcg tcgattttgg cttcgatttc caactcgatt
    34801 tcatcggaaa gcttcacgcc gccttcggcg aagaatttga tgccgttgtc ggaataggcg
    34861 ttgtgcgacg cggaaatcat cacgccggcg gacaggcgca acgcgcgggt cagataagcc
    34921 acgccgggcg tgggcagcgg tccggtctgt accacattca cacccgccgc cgtaaaaccg
    34981 gccaccaaag cggcttccag catatagccg gaaatgcgcg tgtctttgcc gatgaggacg
    35041 gtcggttttt ggtcggtgtc gtgctgcacc aaaacctgcc ccgccgcata gccgagtttc
    35101 aatacaaaat cgggcgtaat cggaaactgc ccgacttcgc cgcgcacgcc gtccgtgccg
    35161 aaatattttt ttgccatgtg ttgctccgag aatgtgaacc gttgtccgag attatacagt
    35221 ctgtttgtgc cctgctgtct gcaccgttga tgccgtctga agccgccccg tccttttcag
    35281 acggcatgaa gtatgcgaac cgctgtttac agattgatgc ccaacgcttc ccacaccttc
    35341 aacgcgtccg ccgtcgcctt cacgtcatgc acccgcacaa tttgcgcgcc gcgcgccaca
    35401 gccgccacag ccgccgccac gctgccgtgt acgcgcgccg ccgcgtcggt ttcgcccgtc
    35461 agttcgccta ccatgcgttt gcgcgatacg ccgatcagca gcggaaagcc tgtttccgcc
    35521 atcaattcgg gcaaatgcct catcagcgcg atattgtgtt gcagggtttt gccgaagccg
    35581 aaacccggat cgagcgtgat gcgttgcggt gcgatgcctg ccgcgatgca ttccgccgcc
    35641 cgcgctttca aataccgcgc cacttcaccg acaacatcct gatatttcgg attaatctgc
    35701 atgttttcag gcaaaccccg catgtgcatc aggcaaatgc ccgtgtccgt ctggcgcgcc
    35761 agcaattcga ccgcaccttc gtcggtcaac gccgccacat cgttaataat atcgatgcca
    35821 ccgagtgcca acgctttttc cataatcacc gtgcggcgcg tgtccaaact gacgggaacg
    35881 ccccaccccg ccacttctgc caatacaggc tcaacccgcg cccattcttc ttcggacgaa
    35941 acataatccg cacccggccg cgtcgattcg ccgccgatgt cgagaatgtc cgcaccctct
    36001 ttcaaaagcc gttcggcgtg cgccaaagct gttcgggcgt tttgcgaata cgcgccgccg
    36061 tcggaaaaag aatcgggcgt gagattcacg atgcccatga ttttcggctt gtccaaaccg
    36121 atttcaaacc gtcctgcctg ccaaacgtgt cgtgccatct gaactcctcc caaaataaaa
    36181 aacagattat atgccgtctg aaaccgtctt gtgcgcttca gacggcaccg ctattcgggc
    36241 ggcagacggc atgttgtccg aatgtctgct ccgcctttga atctgccggt atgcctgcta
    36301 tccgcccgac ttttcaaaac aggttccgac gattccgcac gcgcctgccg cctttgccaa
    36361 gccgtacagg atttcctgcg gcatatcgcg gttccataat cccgtaatat tcgcaatcac
    36421 gggcagatgg ctgatttggc ggactttcac gatggattcg acatccaaac ggtagggatg
    36481 gtctttggta tggctcaaca cgcccgactc gcccagaatc aggtggcggt tgccgcgcga
    36541 gacgacatat tctgcggcat tcaaccaatc ttcggcagaa tggtgcttgt ctttacacag
    36601 caccaaggga atgttcaggc ttccggcttc attcaatacg gcaagatcgg acatcagccc
    36661 gccgcccaaa tacaggatgt ccgcccccgc attcaaagcc gcttcgacat ggcggacgtt
    36721 gcggacgcgc accaatacgg gtttccctgc gccatgcgcc gatgcggtct gttccgccaa
    36781 ccgtctgcac cgtccccgcc cttcatccgc acttgaagtg tcgtataagt ttgccgaagt
    36841 gaaaaacgga tccagaaaca ctgcatccgc attgcgccat actgacggtt ctgcggcgat
    36901 acggacggtt tccccgccgc cgaaagccac gcctttggcg gcaacgcggc tgtcttccgc
    36961 ccgattttcc cgactgacgg ttttccatgt atccaaaatg cggacggctt tctcgacctc
    37021 cggcagcgtc tgcacctccc tgacgctcaa aaccctatcg tcgccgattg cgccgatgac
    37081 agtacgctcg tcgccgtgag aaatgtgttc tcgcagacct ctgctgcgga taaaggcgac
    37141 aacgccggca atgtccgctt cggcggcacg cctgctcatg acaataatca tatttcctcc
    37201 tgacacaaga aacggcctgc ccaaaatagg atttttgcaa gccgtgttat actgtggcgt
    37261 gttttacaga ttgttcgggc tatggattta ttatcggttt tccacaaata ccgtctgaaa
    37321 tatgcggtgg ccgtgctgac gatactgctt ttggcggcaa tcgggctgca cgcttccgta
    37381 tatcgcatcc tcacacctga aaacatcaga agccgcctcc aacaaagcat tgctcatacg
    37441 caccggaaaa tctcgtttga tgcggacatt cagcgcaggc tcctgccccg gccgaccgtc
    37501 atcctgaaaa acctgaccat taccgaaccc ggcggcgacc ggactgccgt ttccgtccaa
    37561 gaaaccaaaa tcggattgag ctggaaaaac ctgtggtcgg atcagataca gattgaaaaa
    37621 tgggtggttt cgagtgcgga acttgccctg acgcgcgacg ggaaaggtgt ttggaacatc
    37681 caagacctga tcgacagcca aaaacgccaa gcctcagtca accgcattat cgtcgaaaac
    37741 agcaccgtcc gcctcaattt cctgcaggaa cagcttatcc tgaaggaaat caacctcaac
    37801 ctgcaatccc ccgattcgtc ggggcagccg tttgaaagtt cgggcatact ggtttgggga
    37861 aagctgtccg tcccgtggaa aagcaggggg ctgttccttt cagacggcat cggcacgccc
    37921 aaaatctcac cgttccattt tgaagcttcc acttcgctgg acggacacgg catcaccatt
    37981 tccaccaccg gcagcccttc tgtccgtttc aacgccggcg gagcggatgc cgccggcctc
    38041 ggcctgcgtg cagacacttc cttccgcaac ctccacctga ccgcccaaat ccctacgctg
    38101 gcactcagga acaacagcat taaaattgaa accgtcaacg gcgcatttac catcggcggc
    38161 gaatatgccc gatgggacgg ttcgttcaaa ctcgacaaag ccaacctgca ctccggcatc
    38221 gccaacatcg gcaacgccga aatctccggc agcttcaaaa caccgcgcca ccagaccaac
    38281 ttctccctca attcgccgct cgtatggacg gaaaacaaag ggctggacgc gccgcgcctg
    38341 tatgtatcga cccttcagga taccgtcaac cgcctgccgc aaccccgttt catcagccgg
    38401 ctcgacggtt cgctgtccgt accgaatctg caaaattgga atgccgaatt aaacggcaca
    38461 ttcgaccgcc aaaccgttgc cgcgaaattc agatacacac atgaagacgc accgcatctg
    38521 gaagccgccg tcgcactgca aaaattgaac ctgaccccct atcttgacga cgtgcggcaa
    38581 caaaacggca aaatatttcc cgacaccctc gccaagctgt ccggcgacat cgaggcgcac
    38641 ctgaaaatcg gaaaagtcca acttcccggc ctgcaactgg acgatatgga aacctacctc
    38701 cacgccgaca aagaccatat cgcgctcagc cgtttcaagt cagggcttta cggcggccat
    38761 accgaaggcg gcatcagcat cgccaacacc cgtcccgcca cttaccgcct gcaacagaat
    38821 gcaagcaaca tccaaatcca accgctgctg caagacctgt tcggcttcca cagcttcagc
    38881 ggcaacggcg acgcggtcat cgacctgacc gcgggcggcg aaacccgaaa agagcttatc
    38941 cgctcgcttc agggcagcct gtcgctaaat atttccaacg gtgcatggca cggtatcgac
    39001 atggacaata tcctgaaaaa cggcatttcg ggcaaaactg ccgacaatgc cgcacccagc
    39061 acacccttcc accgattcac gctcaacagc gaaatttcag acggcatcag ccgccacatc
    39121 gataccgaac tcttctccga cagcctctat gttaccagca acggctatac caatctggat
    39181 acgcaggaat tgtctgaaga tgtccttatc cgcaacgccg tccatccgaa aaacaaaccg
    39241 attcccctga aaatcaccgg tacggtggac aagccgtcca ttaccgtcga ttacggcagg
    39301 ctgaccggcg gcatcaattc gcgcaaagag aaacagaaaa tcctcgaaga caccctgctg
    39361 gaacaatggc agtggctcaa acctaaagaa ccgtaaacat cctgcgtaca aaaatgccgt
    39421 ctgaaacgcc ctcgcgcttc agacggcatt gccgtaaaac ctacaagccc aattcccccc
    39481 aaatcccatc aatcttcgcc gtaaccgcag ggtctttttt gatgacgcgc ccccattcgc
    39541 ggtcggtttc tcccggccat ttgttggtcg catccaaacc cattttgccg ccgagtccgc
    39601 tgacggggct ggcgaagtcg aggtagtcga tgggcgtgtt gtccaccaaa acggtgtcgc
    39661 gcacggggtc catgcgcgtg gttaccgccc agatgacttc tttccagtcg cgcacgttca
    39721 catcgtcatc caccacgatg atgaatttgg tatacataaa ctggcgcagg aacgaccagc
    39781 agcccatcat cacgcgcttg gcgtgtccgg cgtactgttt tttcatgctc accaccgcca
    39841 tgcggtagga gcagccttcg ggcggcaggt agaaatcggt gatttcgggg aactgctttt
    39901 gcaaaagcgg tacgaacact tcgttcaacg ccacgcccaa aacggcaggc tcatcaggcg
    39961 gtttgcccgt gtaggtcgaa tggtaaatcg ggttttcgcg catggtaatg cgttcgaccg
    40021 taaacacagg gaaataatcc tgctcgttgt aatagcccgt gtggtcgccg tatgggcctt
    40081 ccaacgcggt ttcgttcgga tgaatgacgc cttccagtac gatttcggca cgtgcgggca
    40141 cttgcaaatc gttgccgata catttcacca gttccgtccg cgaaccgcgc agcagtccgg
    40201 caaactggta ttcgctcaag gtatcgggaa caggcgttac cgcgcccaaa atggtggcgg
    40261 ggtcgcagcc gagcacaacg gagacgggat acgacgtatc aggattgagt ttgcggaatt
    40321 cctgataatc caacgcgccg ccgcgatgcg acagccagcg cataatcagc ttgtttttgc
    40381 cgatgagttg ttggcggtaa atgccgagat tttggcgttt tttgtgcggc ccgcgcgtta
    40441 cggtcaagcc ccatgttacc agcggcgcaa cgtcttccgg ccagcaatgc tgaatcggaa
    40501 gtttatacaa atcaacgttt tcgccttccc acacgatttc ctgacacggc gcgtttttca
    40561 ccacgttcgg cgccatgctc caaatgtctt tcagtagcgg cagtttggaa aacgcatctt
    40621 tgatgccttt gggcggttcg ggttctttca aatacgccag cgtctgcccg atttcacgca
    40681 gcttggacac gctgtccgcg cccatgccca tcgccacacg ttcgggcgtg ccgaacaggt
    40741 ttgccaacac gggataatcg tagcgcgtac cgtcgggctt aatcgggttt tcaaacaaca
    40801 acgccgaccc ttcggcacgc agcacgcggt cggcgatttc ggtcatttcc aaatgcgggg
    40861 aaacggggtg ggcgacgcgc ttgagtttgc cttgctgctc gagcatgacg atgaagtcgc
    40921 gcaggtcttt gtatttcata tttatccttt ttgtcctttt atcctgaaca atccgattcg
    40981 gataccgccc ctatccttgc ctgtgcttcg gcatattcta tgccgtgata aaagtcgcgt
    41041 accagcggat gtccgccgct ttgatgaagt tgcagcaaag ggcgttgacc atcgggttgg
    41101 gtaacgacat tgcaatgcaa accgaaggtg tcggattcat aagagggcag ccggttgcag
    41161 atcatgccga aataaacggc gttttccgga ttgtcgtgaa agtggctttg atagtcgtta
    41221 aaactctttt cgctgacgga cacccacacg ccatattcca gcgtttcctc atgcccgata
    41281 atcggaatcg gcagcaccgc gcggataaag cggtcggttt ggtcgggata gcggatgatg
    41341 cagaaatcag aatcgcattc tgcttgataa gcaatgcgtt cttcttcact gagttgatta
    41401 tagggatcgg gggcggtaaa gccgattgcg ggcatatctt catgtttttc gccgcaggaa
    41461 gtgcaagtgt acataaggtt tcagactttc aaaacgagtt tgcggtaaag ccattcgccg
    41521 gcaaacagca tccccatcaa tacataggca atcacgcctg tataaaccgc ccaccaatca
    41581 tatcgcccca accgggccaa caaagcggca agcgtcccgt tggcaataaa gaatacgcac
    41641 caaacctgcg ttacccggcg ggtatagcgc acagcttttt caggcaggtc gggctgttgc
    41701 agccgcgcga gcctttctat gaccgtctgt ccggcaaaca ggctgccgcc gaacaccgcc
    41761 aacatcatca gattgacgag gacgggatac caatacatcg aatcatgccg cccgaacacc
    41821 aatactgcgg caaaaaacag tgtaataaac aaagccgcat aacgctgttg gggcagtttg
    41881 gcagtcaggg cgcgcagcag ccacaacccg cacatcgccg ccgccagcca gacaaaccag
    41941 cccgcctccc tgccgtaata tagtggatta acaaaaacca gtacagcgtt gcctcgcctt
    42001 gccgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tataccacaa
    42061 agcgggatag gcaatgctta atacggtcag aaaaatatgt ccgaaaaaac cgggtttcat
    42121 tttgaatccg cacaaatgtt ttcagacggc atccgataaa aacatgccgt ctgaaaaata
    42181 attagaaata cccgattagc ccgcctgaat cttcaatacc gcctgtacca cgtcgttgac
    42241 ggtgcggaca ttgcggaaat cttcagcctg cagcttgcgg gctgccacag ccattcacga
    42301 ttgcgatata cccggcggca ttcccgactg ccgttcagca gaaagcgcac ccattccaaa
    42361 ccgctccaat atgcctcggg cagcataccg gcttcagacg gcatatcgtc cgaagcagat
    42421 aaagtcaggc tgtaacgcgt ccctttggtc agaaccatcg ccaaagcata agcaaacggc
    42481 gcgcgcgttg ccgatacggc atatccttcc ggcagcggat cgtccgccgc caaaaccaaa
    42541 accgacccgc atccctcttc caacagtgat gccgcttccg ccaatgccgt ttccacaccg
    42601 tcggcacaca cggccaatgc cgtctgctcg ctcatatccc gcctcaatat cgaccattac
    42661 cccgccgttg cattgtgcac cgacaaaccg aacgaagtcg gcgacacgat ttgcgatttc
    42721 aacagttcca accacaaatc gaaactgcgc gccatttccc cgtcgtgcga ggcataaact
    42781 atagtggatt aaattttact agattagcag atatgttacc ctcgaaatat gaagataaca
    42841 cactgcaaat taaagaaaaa agtacagaaa gaactgctcc atttttttgt actggaagtt
    42901 accgcccgtt ctgccgccga tattttgggt atccatccca attcggcagc actgttctac
    42961 cgtaaaatcc gcacggttat caaccatcat ttggtcttgg ctgccgatga ggtttttgag
    43021 gatcctgtcg agccggacga aagcgatttc ggcggacggc gtaaaggcag acgtggtcgc
    43081 ggtgcggcag gaaaagtggt tgttttcggc attctgaaac gcaacggacg ggtctatacc
    43141 gttgtcgtaa acaatgccaa gtctgaaacg ttactccctg tcatcaaaaa gaaaatcatg
    43201 ccggacagta ttgtttatac cgatagtctg agcagctgcg acaagttgga cgtgagcagt
    43261 tttatccatt accgcatcaa ccattccaag gaatttgcag accgtcaaaa ccacattaac
    43321 ggcattgaga acttttggaa tcaggcaaaa cgcgtcttgc gaaaatatag tggattggca
    43381 cgaacgtttc cccgaattgg aagcgcaact gggtgcgccg attgaggatt acgcgccgcc
    43441 gaaacattgg acgcgcaagc agctcagaag tatggggcgt gtgtcgtatt tgtgcgtcga
    43501 tgcggcggag caggctttga cggatgccgg tttgctcggg gacgaaagca ttaccgacgg
    43561 acggatgggc gttgcctgcg gctcttccag cggcagcacc aaagacatcg gcgatgtggg
    43621 cgaattgttg ctgaccggca cgtcgcgcaa cttcagtgcc aacacctatg tgcgtatgat
    43681 gccgcacacc accgccgcca atatcggcat ctttttcggg ctgaaaggac gcatcatccc
    43741 gacatcgagc gcgtgttcgt ccggcagcca aggcataggt tatgcctacg aagccatcaa
    43801 atacggtctg accgatatga tgctggcggg cggaggcgaa gaatttttct cgtccgaagt
    43861 gtatgttttc gactcgcttt atgccgccag ccgccgcaac ggcgaaccgg aaaaaacccc
    43921 gcgcccatac gacgcgaacc gcgacgggct ggtcatcggc gaaggcgcgg ggattttcgt
    43981 gctggaagaa ttggaacacg ccaaacggcg cggtgcgata atttacgccg aactcgtcgg
    44041 ctacggagcc aacagcgatg cctaccatat ttccacgccc cgccccgacg cgcaaggcgc
    44101 aatccttgcc tttcagacgg cattgcaaca cgcaggtctt gcgcccgaag acatcggctg
    44161 gattaatctg cacggcaccg ggacgcacca caacgacagt atggaaagcc gtgccgttgc
    44221 agcggttttc ggcaacaata cgccctgcac gtccaccaag ccgcaaaccg gacacacgct
    44281 gggcgcggcg ggcgcaatcg aagccgcgtt cgcgtggggc attgccgacc ggcaaagcaa
    44341 tcccgaaggg aaacttccgc cccggctttg ggacgggcag aacgaccccg acctgcccgc
    44401 catcaacctg accggcagcg gcagccgctg ggaaaccgaa aaacacattg ccgccagctc
    44461 gtcgtttgcc ttcggaggaa gcaactgcgt cttaatcatc ggatgaaata agtttgtcaa
    44521 tcccaccgct atgctataca atacgcgcct actcttgacg ggtctgtagc tcaggggtta
    44581 gagcagggga ctcataatcc cttggtcgtg ggttcgaacc ccaccggacc caccaattcc
    44641 caagcccgga cgtatgtttg ggcttttttg ccgccctgtg aaaccaaaat gctttgagaa
    44701 accttgataa tgaaaaaagt cagcgtattg attgttgcca aaaacgaagc aaaccacatt
    44761 cgggaatgta ttgaaagttg ccgtttcgat aaagaagtta tcgttattga cgaccacagc
    44821 accgacaata ctgccgaaat tgccgagggt ttgggtgcaa aagtcttcag acggcatttg
    44881 aacggggatt tcggagcgca aaaaacattt gccatcgaac aggcaggcgg agaatgggtt
    44941 ttcctgattg atgcagacga acgctgcacg ccggaactat ctgatgaaat ctcaaaaatt
    45001 gtccaaaccg gcgattatgc cgcctatttt gtcgaacgcc gcaacctttt ccccaaccat
    45061 cccgccacac acggcgcgat gcgtcccgac agcgtatgcc gtctgatgcc gaaaaaagac
    45121 agttcggtgc aaggcaaagt acacgaaacc gtacaaaccc cctaccccaa acgccgtctg
    45181 aagcatttta tgtaccatta cacatacgac aactgggaac aatatttcaa caaattcaac
    45241 aaatacactt ccatctcagc cgagaaatac cgagagcagg gaaagcccgt gcgtttcgtt
    45301 agggacatta tcctccgccc gatttggggt tttttcaaaa tttatatcct gaacaaaggg
    45361 tttctcgatg gaaaaatggg ttggattatg tccgtcaacc acagctatta cacgatgatt
    45421 aaatatgtca aactatatta tctgtacaaa tctggcggaa aattttaaat ggaaaaagaa
    45481 ttcaggatat taaatatcgt atcggccaag atttggggcg ggggcgaaca gtatgtctat
    45541 gatgtttcaa aagcattggg gcttcggggt tgcacgatgt ttaccgccgt caataaaaat
    45601 aatgagttga tgcacagacg tttttccgaa gtttcttccg ttttcacaac gcgccttcac
    45661 acgctcaacg ggctgttttc gctctgcgcg cttacccgct ttatccggaa aaaccgcatt
    45721 tcccacctga tgatacacac cggcaaaatt gccgccttat ccatactttt gaaaaaactg
    45781 accggggtgc gcctgatatt tgtcaaacat aatgtcgtcg ccaacaaaac cgatttttac
    45841 caccgcctga tacagaaaaa cacagaccgc tttatttgcg tttcccgtct ggtttacgat
    45901 gtgcaaaccg ccgacaatcc ctttaaagca aaataccgga ttgttcataa cggtatcgat
    45961 accggccgtt tccctccctc tcaagaaaaa cccgacagcc gtttttttac cgtcgcctac
    46021 gccggcagga tcagtccaga aaaaggattg gaaaacctga ttgaagcctg tgtgatactg
    46081 catcggaaat atcctcaaat caggctcaaa ttggcagggg acggacatcc ggattatatg
    46141 tgccgcctga agcgggacgt atctgcttca ggagcagaac catttgtttc ttttgaaggg
    46201 tttaccgaaa aacttgcttc gttttaccgc caaagcgatg tcgtggtttt gcccagcctc
    46261 gtcccggagg cattcggttt gtcattatgc gaggcgatgt actgccgaac ggcggtgatt
    46321 tccaatactt tgggggcgca aaaggaaatt atcgaacatc atcaatcggg gattctgctg
    46381 gacaggctga cacctgaatc tttggcggac gaaatcgaac gcctcgtctt gaaccctgaa
    46441 acgaaaaacg cactggcaac ggcaggacat caatgcgtcg ccaaccgttt taccatcaac
    46501 cataccgccg acaaattatt ggatgcaata taaactgctt tcagacggca tatgccgtct
    46561 gaaagccttt gatgcaacaa accgccaaat tatattcgtt cattggaaag aaacaccccg
    46621 aatccatcct taaaaataag aaaatcccaa tatcccccga tattacgcag cctattggca
    46681 aagttttgca gcgtcttccc cggcttgtgc tgccgcatca agtgctttgt tacaatgtat
    46741 agtagactaa caaaaaccag tacagcgttg cctcgcctta gctcaaagag aacgattctc
    46801 taaggtgctc aagcacca
//
LOCUS       DAWHXS010000002        40466 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWHXS010000002 DAWHXS010000000
VERSION     DAWHXS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.002"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..736)
                     /gene="bamC"
                     /locus_tag="WIE22_000047"
     CDS             complement(<1..736)
                     /gene="bamC"
                     /locus_tag="WIE22_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ4126205.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEA"
     gene            complement(745..1620)
                     /locus_tag="WIE22_000048"
     CDS             complement(745..1620)
                     /locus_tag="WIE22_000048"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ4126206.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGIYRHFKAVAEAAAIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1658..1912
                     /locus_tag="WIE22_000049"
     CDS             1658..1912
                     /locus_tag="WIE22_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126207.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFAN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            1927..3319
                     /locus_tag="WIE22_000050"
                     /pseudo
     CDS             1927..3319
                     /locus_tag="WIE22_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
     gene            complement(3381..4133)
                     /gene="rlmB"
                     /locus_tag="WIE22_000051"
     CDS             complement(3381..4133)
                     /gene="rlmB"
                     /locus_tag="WIE22_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ4126208.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(4199..4687)
                     /locus_tag="WIE22_000052"
     CDS             complement(4199..4687)
                     /locus_tag="WIE22_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ4126209.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(4692..5411)
                     /locus_tag="WIE22_000053"
     CDS             complement(4692..5411)
                     /locus_tag="WIE22_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ4126210.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIAAAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(5415..6356)
                     /gene="miaA"
                     /locus_tag="WIE22_000054"
     CDS             complement(5415..6356)
                     /gene="miaA"
                     /locus_tag="WIE22_000054"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ4126211.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSLTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPSLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            6355..6664
                     /locus_tag="WIE22_000055"
                     /pseudo
     CDS             6355..6664
                     /locus_tag="WIE22_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146840.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6710..6988
                     /locus_tag="WIE22_000056"
     CDS             6710..6988
                     /locus_tag="WIE22_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126212.1"
                     /translation="MFRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKHQTMFLFIPIVLLVVYLFHYFGAF"
     gene            7007..8158
                     /gene="earP"
                     /locus_tag="WIE22_000057"
     CDS             7007..8158
                     /gene="earP"
                     /locus_tag="WIE22_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ4126213.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLH
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCE
                     AVRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWQQAGSPMTLLLAGTQIID
                     SLKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     AQLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRRLSDDLNGGEALSA
                     TQRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            8201..8761
                     /gene="efp"
                     /locus_tag="WIE22_000058"
     CDS             8201..8761
                     /gene="efp"
                     /locus_tag="WIE22_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ4126214.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(8811..9647)
                     /locus_tag="WIE22_000059"
     CDS             complement(8811..9647)
                     /locus_tag="WIE22_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ4126215.1"
                     /translation="MKNKTSSLLLWLTAIMLTACSPNKNDKTKEVGASAASSSASSAS
                     SQSDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            complement(9764..10345)
                     /gene="metW"
                     /locus_tag="WIE22_000060"
     CDS             complement(9764..10345)
                     /gene="metW"
                     /locus_tag="WIE22_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ4126216.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTSNRQVKHFPNLLGSLAFYRVG"
     gene            complement(10342..11481)
                     /locus_tag="WIE22_000061"
     CDS             complement(10342..11481)
                     /locus_tag="WIE22_000061"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ4126217.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            11631..11831
                     /locus_tag="WIE22_000062"
     CDS             11631..11831
                     /locus_tag="WIE22_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126218.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            complement(12043..12168)
                     /gene="ykgO"
                     /locus_tag="WIE22_000063"
     CDS             complement(12043..12168)
                     /gene="ykgO"
                     /locus_tag="WIE22_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ4126219.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(12168..12443)
                     /locus_tag="WIE22_000064"
     CDS             complement(12168..12443)
                     /locus_tag="WIE22_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ4126220.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            12643..13521
                     /gene="metF"
                     /locus_tag="WIE22_000065"
     CDS             12643..13521
                     /gene="metF"
                     /locus_tag="WIE22_000065"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ4126221.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            13659..15935
                     /gene="metE"
                     /locus_tag="WIE22_000066"
     CDS             13659..15935
                     /gene="metE"
                     /locus_tag="WIE22_000066"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ4126222.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTGFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKNQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            16214..16951
                     /locus_tag="WIE22_000067"
     CDS             16214..16951
                     /locus_tag="WIE22_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ4126223.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            17200..18603
                     /locus_tag="WIE22_000068"
     CDS             17200..18603
                     /locus_tag="WIE22_000068"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ4126224.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDSFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAS
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            18882..19259
                     /gene="sdhC"
                     /locus_tag="WIE22_000069"
     CDS             18882..19259
                     /gene="sdhC"
                     /locus_tag="WIE22_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ4126225.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            19253..19594
                     /gene="sdhD"
                     /locus_tag="WIE22_000070"
     CDS             19253..19594
                     /gene="sdhD"
                     /locus_tag="WIE22_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ4126226.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            19597..21360
                     /gene="sdhA"
                     /locus_tag="WIE22_000071"
     CDS             19597..21360
                     /gene="sdhA"
                     /locus_tag="WIE22_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ4126227.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            21481..22188
                     /locus_tag="WIE22_000072"
     CDS             21481..22188
                     /locus_tag="WIE22_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ4126228.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            22192..22440
                     /locus_tag="WIE22_000073"
     CDS             22192..22440
                     /locus_tag="WIE22_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ4126229.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            22552..23835
                     /gene="gltA"
                     /locus_tag="WIE22_000074"
     CDS             22552..23835
                     /gene="gltA"
                     /locus_tag="WIE22_000074"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ4126230.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            24040..26868
                     /locus_tag="WIE22_000075"
     CDS             24040..26868
                     /locus_tag="WIE22_000075"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ4126231.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGSADEAMLKKQVSVLRLISAYRIQG
                     VGAAKLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            26968..28179
                     /gene="odhB"
                     /locus_tag="WIE22_000076"
     CDS             26968..28179
                     /gene="odhB"
                     /locus_tag="WIE22_000076"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ4126232.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
                     AAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKY
                     KEKFEKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRG
                     LVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTP
                     IINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDA
                     LEDPARLLLDL"
     gene            complement(28231..28347)
                     /locus_tag="WIE22_000077"
                     /pseudo
     CDS             complement(28231..28347)
                     /locus_tag="WIE22_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            28544..29977
                     /gene="lpdA"
                     /locus_tag="WIE22_000078"
     CDS             28544..29977
                     /gene="lpdA"
                     /locus_tag="WIE22_000078"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ4126233.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNSDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELLTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            30072..30359
                     /locus_tag="WIE22_000079"
     CDS             30072..30359
                     /locus_tag="WIE22_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126234.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            30458..31624
                     /gene="sucC"
                     /locus_tag="WIE22_000080"
     CDS             30458..31624
                     /gene="sucC"
                     /locus_tag="WIE22_000080"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ4126235.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            31635..32525
                     /gene="sucD"
                     /locus_tag="WIE22_000081"
     CDS             31635..32525
                     /gene="sucD"
                     /locus_tag="WIE22_000081"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ4126236.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            32554..32694
                     /locus_tag="WIE22_000082"
     CDS             32554..32694
                     /locus_tag="WIE22_000082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126237.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFEAGRIFIGQTGA
                     NP"
     gene            complement(32760..33089)
                     /locus_tag="WIE22_000083"
     CDS             complement(32760..33089)
                     /locus_tag="WIE22_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4126238.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            complement(33490..34692)
                     /gene="dcm"
                     /locus_tag="WIE22_000084"
     CDS             complement(33490..34692)
                     /gene="dcm"
                     /locus_tag="WIE22_000084"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4126239.1"
                     /translation="MLIPQTSLFDQENNRIISQRKLLEQSLLQGIPLNELIRQNQMSY
                     DDLIYIHHDKKPFEKRKNLVLSDFQKKENHIPIISFFSGAGGLDLGFEAAGFQHSMLI
                     EKIPLFCDTLRLNRPNWSVFNADVSETEEMVELIRNNIGSLSFDGVFIGGPPCQPFSI
                     ASNQRFSKTDKNFKRVGFEHKQNGNLLFNYVELIKIFRPKVFLIENVSGLMDIDNGKQ
                     LASVYCDLQQQGYSLYKPQIINSEYFHVPQQRIRLFIIGYLGDKKFSPLQPTNTPLAC
                     GNTFEIPIINQHNHITREHSAESILRYMELAYGQRDKKGRVDRLDPSKPSKTIIAGGT
                     KGGGRSHLHPYIPRTLSVRESARLQTFPDDYIFTGAVARQFTQVGNAVPPILAAQLAT
                     AIKHSYFE"
     gene            complement(34705..35889)
                     /locus_tag="WIE22_000085"
     CDS             complement(34705..35889)
                     /locus_tag="WIE22_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4126240.1"
                     /translation="MIEWRFDQGRLDYFRFDEIKKIAKGLYGINQCPKPRAKDIDIVR
                     LSLTQETNLPFAPNSSQYPVWRNYGRVFKCLLLATEHNGLICTTDLCADLVQNDEISC
                     DDYLLHFATHFSYPSPAFSGYQPSENRVFPVLAIIRALISKYKETGVNFLDMDEIIYK
                     IASNNSITGIASFSFSQLIMNKEAIKYDPRQIRELVKFISQFSFLSWYENKLFLDITD
                     FSELHLLENQFIADTQPQERLDSLEIMRLGKTNNNFLLMNEIVSLSIDDQAFSEGGKI
                     KVTHLITERNSILRKIYFSKTQNAQICRMCGLDTQRSYEWSEHIIEVHHLLPLSSPVR
                     VEDKTTSLKDLVGICPTCHRATHKYYTFWLNLHKKKDFVSYEEAYHVYEEAKSKVKRI
                     YK"
     gene            complement(35931..38780)
                     /gene="uvrA"
                     /locus_tag="WIE22_000086"
     CDS             complement(35931..38780)
                     /gene="uvrA"
                     /locus_tag="WIE22_000086"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ4126241.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSITGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDSGGRIIAQGSPEEVAKVKGSYTGKYLKVVLG"
     gene            38935..39732
                     /locus_tag="WIE22_000087"
     CDS             38935..39732
                     /locus_tag="WIE22_000087"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ4126242.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            complement(39877..39984)
                     /locus_tag="WIE22_000088"
                     /pseudo
     CDS             complement(39877..39984)
                     /locus_tag="WIE22_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         9694 a         9833 c        10736 g        10203 t
ORIGIN      
        1 cggcttcgag gttgggatcg gacggggagg gctgccatac ggtcgtgtct ttgtctttgc
       61 cgccgtacac ttctttcatg gctttgtggg cgaagaagat gtcggaaacg ccgtttttgc
      121 cctgttcgat acggacgatg aatttgtcgc gctcgccggt ggagtagatg ccgcccaaac
      181 cgactgtgtc gaataggcgg cgcaagctgt cttgggggat tttggcacgg ttttccgccc
      241 actcggtttc catttgtccg atggcgggtt cttcggattt gatgtcgaag ccgttttcct
      301 gccaaaaggc tttcagaagc ggccagattt cggcagggga tttgccgtca acgacaagcc
      361 aacgttggct gccgtcgcgc tcgaggcgga cacctttgac gcttttcaat acttcggcat
      421 cggcaggctg ttggacggcg ggtgtgcggc gtttttccaa atcgctggcg cggacggcgc
      481 ccgaaccggc aggcaggcgg tagaggctgc cttggtcggg gttgttcaaa tcgggtggga
      541 cttcaagttt gatcaggcgg tgcgaccggc tttggtagtc gagcttgggc tgttcggttt
      601 tgctgccgga gcaggcggca agcccgatga gtgcgagcgc ggcaatgacg ggtttgatat
      661 gggtcatcgt gtcatcctgt gtgatggata ttaaagtgtt tgttgcgtta tgccgtccga
      721 acggttcaga cggcatagtt atatttagag ttgtcctgag gctttcaggg cggcgcggac
      781 ttttgcctgt ccgccttccg tcagcggaac gagcggcagg cggacgtgcg gttcgcatct
      841 gcccagggcg gataccgccc atttcggtgc ggcggggctg ggttcgcaga acatggtgtc
      901 gtaaatcgga atcagccggt cgttgagttc gcgtgcaagg gcgatatcgc cttgaagcgc
      961 ggcgcggcac atatcggcaa agagcttggg cgcggcgttg gcggctacgg taataacgcc
     1021 gtgtccgccg cagagcatga acggtagggc ggtgtggtcg tcgccggaca ggacgacgaa
     1081 gccttcgggc gcgcggttga tgagttcgat gttgctgccg atgttgccgc tggcttcttt
     1141 cacgccgacg atgttgggga tttcggcaag gcgcaggata gtgtcgttag tcatgctgac
     1201 gacggtacgg ccgggcacgt tgtagataat catcggaatc gcggcggctt cggcaacggc
     1261 tttgaaatgg cggtaaatgc cttcttggga gggcttattg taatagggga cgacggataa
     1321 ggtgtagtcc gcgccggctt tttcggcggc ttgggaaagg gcgatggctt cgacggtgtt
     1381 gttcgcccct gtgccggcga tgacggggac gcgtttggca acgtgtttga cgacggcttc
     1441 gataacggcg gtgtgttctt cgacggagag ggtggcggat tcgcctgtcg tgccgacggc
     1501 gacgatgccg tccgtgccgt tttcaatgtg ccagtcgatt aagtcgcgga gttgttcgta
     1561 atggatgctg ccgtcttgat tcatcggggt aatcagggca accaggctac cttgtaacat
     1621 acagaacctt ttatcagttg tggtgtaggg ggcggtaatg cttccgattg tagcctactt
     1681 taccgcaggt gtgaaatccg gcgggtccgg tatgcacggg gcggcttggc ggcggatgcg
     1741 tcgggtttgt atttttgtgt ttgaaatcag gttattgcgt tttgcaaacg atattccggc
     1801 ggcgacggtt cggaatctgt ttgccggacg ggcgcgtatg ccgtccgaaa tcgaaattga
     1861 agcggtatgg cggattcgct acaatccgcg ctttggtttt ttcaacattt gaagcaagga
     1921 aacattatga gcggtcatat gggaaaaggg gcggatgcgc ctgatttggt gtacggtttg
     1981 gaggataggc cgccgttcgg taatgcgctg ttgagcgcgg ttacccatct tttggcgatt
     2041 ttcgtgccga tgattacgcc cgcgctgatt gtgggcggcg cgctggaact gccggtggag
     2101 atgacggcgt atctcgtgtc gatggcgatg gttgcgtcgg gtgtcggcac ttatttgcag
     2161 gtcaaccgct tcgggccggt cggttcgggg atgctgtcta tccagtcggt gaatttctcg
     2221 ttcgttaccg tcatgattgc gctgggcgcg gggatgaaag agggcggttt gactaaggat
     2281 gcgatgattt cgacgctctt gggcgtatcg tttgtcggcg cgtttttggt gtgtttctcg
     2341 gcgtggcttc tgccgtattt gaaaaaagtg attacgccga cggtcagcgg tgtggtggtg
     2401 atgctgatcg gcttgagttt ggtacacgtc ggtattaccg atttcggcgg cggcttcggc
     2461 gcaaaggcgg acggcacgtt cggctcgatg gaaaacttgg ggctggcatc gctggtgctg
     2521 ctgattgtgc tggtgttcaa ctgcatgaaa aacccgctgc tgcggatgag cggcattgcg
     2581 gtcggtctga ttgccggcta tatcgtcgcg ctgtttttgg gcaaggtgga tttttcggca
     2641 ctgcaaaacc tgccgctggt tacgctgccc gtaccgttta aatatggttt tgcttttgac
     2701 tggcacgcat ttattgtggc gggtgcgatt ttcctgttga gcgtgtttga ggcggtcggc
     2761 gatttgaccg caacggcaat ggtgtccgat cagccgattg aaggcgagga atacaccaaa
     2821 cgcctgcgcg gcggcgtgtt ggcagacggc ttggtgtcgg tgattgcgac ggctttgggt
     2881 tcgctgccgc tgacaacgtt tgcgcaaaac aacggcgtga ttcagatgac cggcgtggct
     2941 tcgcgccatg tgggcaaata tattgccgtg attttggtgc tgttgggtct gttcccccgt
     3001 tgtcggacgc gcgtttacga cgattccgag tccggtttta ggcggcgcga tggttttgat
     3061 gttcggcttg attgcgattg cgggtgtgcg gattttggtc agccacggca tccgcaggcg
     3121 cgaggcggtg attgcggcga cttcggtcgg cttgggcttg ggcgtggcgt ttgagccgga
     3181 agtgtttaaa aacctgcctg tcttgttcca aaactctatt tccgccggcg gcattacggc
     3241 agtcttgctg aatttggtct tgcccgaaga taaaaccgag gcggcggtca agtttgatac
     3301 cgaccacttg gaacactgat tttgaaaacg aatgccgtct gaaacagaat ccctgtttca
     3361 gacggcattg tttttgaggc ttacgctttt tcgtttttta atacgcgctg ccggcgggtt
     3421 tcgcttaata ccattccggc agacacggag acgttcatgc tttcgaccgt gccgaacatg
     3481 ggtatggaca ccagcatgtc gcaatgttcg cgtgtcaggc ggcgcatacc gtcgccttcg
     3541 ttgcccatca cccatgccgc gctgtcgggc agatcgcaat ggtaaaggtc ggcgttgccg
     3601 cccatatcgg tgccgatgat ccaaatgccg tattctttca gctcgcgcag ggtgcgggcg
     3661 aggttggtta cggtgatgta ggggacggtt tccgccgcgc cgctggcgac tttgctgacg
     3721 gtggcgttca gccccgcgct tttgtctttc ggtgcgatga cggcatgtac gcccattgcg
     3781 tcggcggtac gcaggcacgc gccgaggttg tgcggatcgg tgatgccgtc gagtatcagc
     3841 agcagcggcg gttcgcttaa gttttccaat acgtcttcga ggtggacgtg gtttttggag
     3901 gcatcgataa atccgaccac gccctgatgg cgcgcacctt tgctgatggc gttgaggcgg
     3961 tcggcatcgg caaaatatac gcggatgttt tcgtttgccg ccttttccaa cacttcgcgc
     4021 gtgcgtacat cgtttttgcc ttcttggaca tagagttcga cgatggattt ggggttttgc
     4081 cacaatcggg cgttgacggc atggaagccg tagataggtc tttggtttgc catatggtgc
     4141 tttgtgaaaa gggttcagac ggcattatag caatttgccg gtatgccgtc tgaaggggtt
     4201 aaaacaggta ggcgatgtat ttcaccaaca ggataaacaa gatggatacg gcgcagccga
     4261 ttttgaatgt cgtaccgacg ataagcccca acagcgtacc caagcccgct ttacctgcct
     4321 gaagcatatt gcgccgatcg atcagttcgc ctgccgccgc gccgataaag ggaccgagta
     4381 ttagtccggg aagggagaaa aatatgccga tgatgctgcc gaccaatgcg ccgcgaacgg
     4441 cgagcttgcc cgctccggta tattttgtcc cccatatgcc tgccacatag tccgccagta
     4501 tgccggcaag gctgatgagt ccgaccgtcc acaaaacgcc cgcgccgtag atttggtagc
     4561 cgccggagta ggcgagcagc caggttccgg caaacatcaa tgccaatccg ggcagggcgg
     4621 ggtaaacgat gcccgccgta ccggcggcta tcagggcgag ggcgaggatg acgagcaagg
     4681 cggtcatagg ttcaaccttt tcttttgttt tggaaaaaac ggcttaatac ggcgcggcat
     4741 tcttcttgca ggattccgcc ccgtatggcg gtgtgcgtat tgaggcgttt gtcggcaaac
     4801 aggttgacga tgcttcctgc cgcgccggtt ttgggttctg ccgcaccgta gatcacgcgc
     4861 ctgattcgtg cctgtatcag tgcggacgcg cacatggcgc agggttcgag ggtgatgtag
     4921 atgtcgcatc cgtcaaggcg gtagttttgc atttcgctgc ctgcctgtgc cagggcgttg
     4981 atttcggcgt ggcggctgac attgcagtcg gcaatgcagg tgttgtgtgc cgccgcgatg
     5041 attttgccgt ctgaaacgat gactgccccg acgggtattt cgccgtcggc ggcggattgt
     5101 tctgcttggc gcagtgcttc gcgcatgaag tgttccattt cttcctgcgg cggaaaggcg
     5161 gcgacgggcg gatggttttt caattcggca agcaggcgag ctttatgcgc ttgggacatt
     5221 tcttgcggcg gcgtgccgtc cagcagcgac tcgagttgcc acagcgtgct tttcgtgagg
     5281 gtcaaacccg atgctttgag cagcagaaag gctttgaccg aaccgttttg ccgcagttct
     5341 tcgagcgtac ggataccgag cctgtgcagg gcggcgacgg ttttgggggc gagcggcggt
     5401 gtggtcagca tggtttatgc gccgaaaaac cgttttgccg cctcaatcag gcgtgtgcat
     5461 gaagtgccgt ctgaaaacgg gtcggcaacg cagtctaaag gtgttttgcg caaccaagtc
     5521 agttggcgtt tggcaagttg gcgggtggcg gcaatgcctt tctcgacaaa tgccgggaaa
     5581 tcggtttttc catccagata tttccatgcc tgacggtagc cgacgcagcg gatggcgggg
     5641 gagtcggcgg tcaggctggg atagcggcgg cgcaggtttt ccacttcgcc gataaagccc
     5701 tgttcaagca tcaggtggaa acgcagggcg atgttttcat gcaggcgggc gcggttttcg
     5761 ggaatcaggg cggcggtatg caaatcaaaa gggagcgtat gggaggtcag gctgccgaga
     5821 tgtgtgctca tcggtttgcc ggttaaataa taaacttcca aagcgcgtcc gatacgctgg
     5881 ctgtcgttcg gtttcagacg gcatgcggtt tcagggtcga ctttttgcag ggtgcggtag
     5941 aggaaatcca agccgtacat ctgtttttgt tcgtccaagt cggcacgcag gcaggcgtcg
     6001 gcttcgggca aatcgttcaa accttgggtc agggcgcgga aatacatcat cgtgccgccg
     6061 acaataaggg caaacctgcc gcgtgaggaa atttccccga ccaagcgcgt gcagtcttcg
     6121 acaaagcggg cggcgctgta tgattcggta ggcgggatga tgtcgataag gtggtgcggg
     6181 acaaaggcgc gttcggaggc ggacggtttc gccgtgccga tgtccatatc gcggtaaacc
     6241 agcgcggaat cgaggctgat gatttcgaca ggcagggttt cggcaatttt gagggcgagc
     6301 gcggttttgc ctccggcggt cggcccgagc agggcaaagg ctttcggggt cggcataacg
     6361 tttcaggttt ggaaaaatac ggattatagc ggaaagcgtg ccgacgttat attttggttt
     6421 gcggaagcac gccgacggca agggggcgtg tttaccgtat gcctttataa aatttcaaac
     6481 cgacgcgccg ggttttcaat atgcccgcgc ccgatgcagc cttgtcctca ggcatcggcg
     6541 gcagcgtccg atttttcggg gaacgcccgt ctcgggcgta tttaaaggtt cggcggtgcg
     6601 gtgttttcct gcggcaaggc ttcagacggc atcgtcggcg catccgttag acaaggcgcg
     6661 tgcttggggc gataatggcg ttttgctttt tttgtttgaa agccttgcga tgttccgaaa
     6721 cctgcttgtc cgctggcttg ccgtctgcct gatacccttg gcgacgcttg ccgttttcgc
     6781 cgccaatccg cccgaagaca aaccccagca tctgattaac ggcatcatcc ttgcctgcga
     6841 agcgacgttt ctgttcaaat tcgtcctttt cgacaccatc aagcatcatt tgaaacaaga
     6901 gtttgatttg aaacatcaaa ctatgtttct gtttattccg attgttttgc tggttgtgta
     6961 tttgttccat tattttggcg cattttagct tgttttctag atggttatga atacatacgc
     7021 ttttcctgtc tgttggattt tttgcaaggt catcgacaat ttcggcgaca tcggcgtttc
     7081 gtggcggctt gcccgtgttt tgcaccgcga actcggttgg caggtgcatt tgtggacgga
     7141 cgatgtgtcc gccttgcgcg cgctttgccc tgatttgccc gatgttccct gcgttcatca
     7201 ggatattcat gtccgcactt ggcattccga tgcggcagat attgataccg cgcctgttcc
     7261 cgatgccgtc atcgaaactt ttgcctgcga cctgcccgaa aatgtgctgc acattatccg
     7321 ccgacacaag ccgctttggc tgaattggga atatttgagc gcggaggaaa gcaatgaaag
     7381 gctgcacctg atgccttcgc cgcaggaagg tgttcaaaaa tatttttggt ttatgggttt
     7441 cagcgaaaaa agcggcggac tgatacgcga acgcgattac tgcgaagccg tccgtttcga
     7501 tagcgaagcc ctgcgtcagc ggctgatgct tcccgaaaaa aacgcccccg aatggctgct
     7561 tttcggctat cggagcgatg tttgggcaaa gtggctggaa atgtggcaac aggcaggcag
     7621 cccgatgaca ctgttgctgg cggggacgca aatcatcgac agcctcaaac aaagcggcgt
     7681 tattccgcaa gatgccctgc aaaacgacgg cgatgttttt cagacggcat ccgtccgcct
     7741 cgtcaaaatc cctttcgtgc cgcaacagga cttcgacaaa ctgctgcacc ttgccgactg
     7801 cgccgtcatc cgcggcgaag acagtttcgt gcgcgcccag cttgccggaa aacccttctt
     7861 ttggcacatc tatccgcaag acgagaatgt ccatctcgac aaactccacg ccttttggga
     7921 taaggcacac ggtttctaca cgcccgaaac cgcatcggca caccgccgcc tttcagacga
     7981 cctcaacggc ggagaggctt tatccgcaac acaacgcctc gaatgttggc aaatcctgca
     8041 acaacatcaa aacggctggc ggcaaggcgc ggaggattgg agccgttatc ttttcgggca
     8101 gccttccgca tccgaaaaac tcgccgcctt tgtttcaaag catcaaaaaa tacgctagaa
     8161 tagcgcgttt tacgacaacc gatttgattg gaaaatcaca atgaaaacag cacaagaact
     8221 gcgcgccggc aatgtattta tggtcggcaa cgatcctatg gtcgttcaaa aaaccgaata
     8281 catcaaaggc ggccgctctt ccgccaaagt cagcatgaaa ctgaaaaacc tgctgaccgg
     8341 cgcggcttcc gaaaccattt acaaagccga cgacaaattc gacgtggtca tcctgtcccg
     8401 caaaaactgt acgtacagct actttgccga cccgatgtac gtctttatgg atgaagaatt
     8461 caaccaatac gaaatcgaag ccgacaacat cggcgacgcg ttgaaattca tcgttgacgg
     8521 tatggaagac caatgcgaag ttaccttcta cgaaggcaac cccatttctg tcgaactgcc
     8581 caccatcatc gtgcgcgaag tcgagtacac cgagcctgcc gtcaaaggcg acacttccgg
     8641 caaagtgatg aaaaccgcgc gcctggtcgg cggcaccgaa atccaagtga tgtcttacat
     8701 cgaaaacggc gacaaagtcg aaatcgatac ccgcaccggc gaattccgca aacgcgcttg
     8761 atttgttgca ttgaaaaatg ccgtctgaaa acgtttcaga cggcattttt ttatcttcgc
     8821 cccaagtttg gattgaagta gatgtttttt tcgtaaacga caatacgcgt gattttgcca
     8881 ttttcgtcaa aatggatgtc taaaaacggt ttgtcgggat tgcgttcacg gttggacagc
     8941 cggaagagtg attggttttc aagcatttct ccgtgtattt tcagatagcc gggcaattgg
     9001 ccgatgtatt ggaaattgcc gccaagtccg ttgttgtgcc ggctcgaata ggcaggggtt
     9061 gccgaaactt caaaataacg cgtgcgtttc ggctcctcgc cttgtccgcg ggtttcttga
     9121 ctgccctgta tcagcaacag cgtcgcttct cggaggcggc gttcctgttc catcaaggca
     9181 tcttgtgcct gccggctgat cgtgcgcttt ccgtaacgca agtcttcaat tgccagtatg
     9241 attttctgat ggtagtcggg atcttcaggg cggatgtcgg gaaacagcag ccgctgtatg
     9301 ttgtcatagc cgccgccggg taagccgaaa cgggtttcca gttcgtgatg ggaaagggca
     9361 aacagacagt ccgagtcgat gtgttcggcg atttgctgca tgagatcgtc aatgccggtg
     9421 gcggggtgca ggtcggtgca atttgacggc ggcgcgcttt ctgcctgctt gacgttatca
     9481 ggggcggatg cggccggttg caaatcggat tgggaagaag ctgatgacgc ggaggacgaa
     9541 gcagcagatg caccgacttc tttggtttta tcgtttttgt tcggagaaca cgcggtcagc
     9601 atgattgcgg taagccataa gagaagtgat gaggttttgt ttttcattct attgtttcca
     9661 gtattaaaga ggccgtctga aaacctaccg tttcattttt cagacggcct gttgttaata
     9721 gaaccgaaga acctgttatt gccgacaagg ttctcaacct gtcttacccg acgcggtaaa
     9781 acgccaggct gcccaaaagg ttggggaaat gtttgacctg tctgttgctc gtcatcacgg
     9841 cgcgttcgag gacgcggatg ttgtttttgg cgcacaacaa atcaaaatct ttgagcgtgc
     9901 accaatggat attgggcgtg tcgtaccaat ggtagggcat acgttcggaa accggcatat
     9961 gtccgccgag tgcgatttgg acgcggttgc gccagtagcc gaaattcggg aagctgacaa
    10021 tcgcctgttt ggcaacgcgc atcaggcagc gcaggatttt ttcggtattc tgcatcgctt
    10081 ggatggtttg gctcaacaca atcacatcaa aactttgatc gttgaatgcg gttaaacctt
    10141 cttccaaatc ggcttggata acatttacgc cgcgcgacat cgcggcgatg acgctgtcgg
    10201 tgtcgatttc gatgccgtag ccgctgcatt ttttgtgttc gaccaatgcg gcaagcagtt
    10261 cgccgtcgcc gcagcccaag tccaagacgc ggctgccttc gggtatccgg tcgtaaatca
    10321 gttgcaaatc atcgcgcagg ttcattgctg acattcctta taaacgttgt tcatataggc
    10381 ggcgaccgca cgcatatagg cttcgtcttc cattaaaaag gcatcgtgcc cgtgtgcgga
    10441 tttgacttcg atatactgca cggatttttg ggcggcaatc agggccttga ccagttcgtg
    10501 cgaacgttcg ggcgcgaaac gccaatcggt gctgaagctg gcgacaaaga attttgcctg
    10561 aacatcctgc acggcgcggg tcaggctgtt gccgaaatcc gccgccggat cgaaatagtc
    10621 caaagccttg gtcatcagca aataggtgtt ggcatcaaac cgcccgacga atttgtcgcc
    10681 ttgatagcgc agataggatt ctacttcaaa ttcaacgcca tagccgtatt gatagccgtt
    10741 ggaacgcaaa tcgcgtccga attttttgcc caaaccgtct tcggcaagat aggtgatgtg
    10801 ccccatcatg cgggcaatcc gcaagccccg agcgggaacg gtgttgcggc tgcggtaatg
    10861 tccttcgttg aaatcgggat cggtcaaaat cgcctgacgc gccacatcgt tgaacgcgat
    10921 attttgcgtg gacagtttcg gcgcggacgc aatcaccaag gcgtggcgca cgcgttcggg
    10981 ataggaaatc gcccactgca aagcctgcat gccgcccaag ctgccgccga caaccgccgc
    11041 ccattgcccg atgccgagat aatcggcaag cgcggcttgg gattttaccc agtccttcac
    11101 cgtaaccagc ggaaaatccg cgccgtattc cctgcccgtt tcaggattaa tcgacaaagg
    11161 cccgctgctg ccgtcgcagc cgcccagatt attcaaaccg accacgaaaa aacgttccgt
    11221 atcaatcggt ttgccaggtc cgaccatatt gtcccaccag cccgtatatt tatcttccgc
    11281 cgaatgcctg cccgcaacat gatggttgcc cgacagcgcg tggcagatta aaaccgcatt
    11341 gtttttttca gcattcagct cgccgtaggt ttcaatcatc agatcgaaac gcggcaaagt
    11401 tttaccgttt tccaaaacca gcggcatctc aaacggaatt ttttggggca ttacaatgcc
    11461 caccgaggca ttttgactca tatcctgttc caacaaatgc ggcgaaaagc gttattatat
    11521 cgcaaacggc atgacttttt gacacggtcg gacaagcagc cggacgcgtt tgaccctcat
    11581 ccgccgcaca cgaatcatac tttttcagac gacctccacc gcttcccgac atgataggca
    11641 gacttttccg tatttttttc tttttcgcac ttgccgcgtt gattatcaac cgccttttca
    11701 gccgcaggca aaaacgcgcc ctgcgcgaag tcgccgaaat cagcgcatgg gtactgctcg
    11761 gtgcagccgc cgcgatgctg ttttggtatc tgtttatgct gtatttcaaa cacattccgg
    11821 attcgtattg acggaaaaaa tgccgtctga aacgcatttt tctgtttcag acggcatatt
    11881 tgatgaaaag ggcttgcggt aggaggtgct ttatagtgga ttaaatttaa accagtacgg
    11941 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
    12001 tttgttaatc cactatacaa ccgaagcagg aagggcaggg agtcagcgtt ggcgcgcttt
    12061 aaaacgcgga ttgcttttgc agatgacgta aactttgccc ctgcgtctga cgatttggca
    12121 gtcgcggtgg cgttgtttgg cggttttgag tgaagacaga acctgcatta tttgtccttt
    12181 ctaaacgatg acatgacgga ttggaaacgt tggttgaact tgctggcgcg tccttcggtg
    12241 ttgacgttac gctgcttgcc ggtatagacg ggatgggatg cggaagaagt atccagcgaa
    12301 aacagcggat attctttgcc gtctgtccaa accatcgttt ttccgtgtgt ttcggcacag
    12361 gagcggatta accagccttc attggcgctg ctgtcgaaaa aaaggacggt tcgataattg
    12421 tcaggatgaa tatttggttt catatattgc cttgctttca gtgttataac ataacaaact
    12481 ctagcatagt ttagaagggc tgtacaagga aatttaacta tttttgtaat atattagaaa
    12541 ttttcatgat aaatctgaaa attttgaaat tgactcatgt ttggcgcaac tttattatgt
    12601 tgcctgaaac atcatataaa agataataaa aggtacgcag ccatgaatta cgcaaaagaa
    12661 atcaatgcgt taaataacag tctttctgat ttgaaaggcg acattaacgt ttcgtttgaa
    12721 tttttcccgc cgaaaaacga acaaatggaa acgatgctgt gggactccat ccaccgtctg
    12781 caaaccctgc atcccaagtt cgtatccgta acttacggcg caaattccgg cgaacgcgac
    12841 cgcacgcacg gcatcgtcaa acgcatcaaa caggaaaccg gcttggaagc cgcgcctcac
    12901 ctgaccggta tcgacgcttc tcccgacgaa ttgcgccaaa ttgccaaaga ttattgggac
    12961 agcggcatcc gccgcattgt cgccctgcgc ggagacgagc cggccggtta tgagaaaaaa
    13021 ccgttttacg ccgaagactt ggttaagcta ttacgctccg tcgccgactt cgacatctct
    13081 gtagcagcat atcccgaagt gcatcccgaa gcgaaatccg cacaagccga cctgattaat
    13141 ttgaaacgca aaatcgatgc gggcgcgaac cacgtcatca cccaattctt cttcgatgtg
    13201 gaacgctacc tgcgcttccg cgaccgctgc gtgatgttgg gtatcgatgt ggaaatcgtc
    13261 cccggtattt tgcctgttac caacttcaag cagctcggta aaatggctca agtaaccaac
    13321 gtcaaaatcc caagctggct gtcgcaaatg tatgaaggtt tggacgacga ccaaggtacg
    13381 cgcaatctgg tggcggcaag tatcgccatc gatatggtca aagtcctgtc ccgcgaaggc
    13441 gtgaaagatt tccacttcta tacgcttaac cgcagcgagc tgacttacgc catttgccat
    13501 attttaggcg tgcgccctta aagccgtatc aaacagtttc agacggcatc taaggtgtct
    13561 aaaaagcaaa acaccgcccc atccgagcca ttctgattta caataccggc cgattcggat
    13621 tgaaccggtc cttacaaaat ccaactggag agttcaacat gacaacatta catttctcag
    13681 gcttcccgcg tgtcggcgcc ttccgcgaat tgaaattcgc acaagaaaaa tactggcgca
    13741 aagaaatcag cgagcaagaa ttgctggctg ttgctaaaga cttgcgcgag aaaaactgga
    13801 aacaccaggt cgctgccaac gccgatttcg ttgccgtagg cgatttcact ttctacgacc
    13861 acatcctcga cctgcaagtc gccaccggcg cgattcccgc ccgcttcggc ttcgacagcc
    13921 aaaacctgtc tttggaacaa ttcttccaac tggcgcgcgg taacaaagac caattcgcta
    13981 tcgaaatgac caaatggttc gacaccaact accactactt ggtgcctgaa ttccacgccg
    14041 ataccggatt caaagccaat gccaaacact atgttcaaca actgcaagaa gcccaagccc
    14101 tcggtctgaa agccaaaccg accgttgtag gtccgttgac tttcctgtgg gtgggtaaag
    14161 aaaaaggcgc cgtcgaattc gaccgtctga gcctgttgcc taaactgttg cctgtttacg
    14221 ttgaaatcct gactgctttg gttgaagccg gtgccgagtg gattcaaatc gacgagcctg
    14281 ctttggctgt cgatttgcct aaagaatggg tggaagccta caaagacgtt tacgctactt
    14341 tgagcaaagt caacgccaaa atcctgttga gcacttactt cggttctgtt gccgaacacg
    14401 ccgcattgtt gaaagccctg cctgttgacg gtctgcacat cgacttggta cgcgcccccg
    14461 agcaactgga cgcgttcgcc gactacgaca aagtcctgtc tgccggcgtg attgacggcc
    14521 gcaacatttg gcgcgccaac ctgaacaaag ttttggaaac tgtcgagcct ctgcaagcca
    14581 aactgggtga ccgtttgtgg atttccagct cttgctcgct gctgcacact ccatttgact
    14641 tgtcagttga agaaaaactg aaagccaaca aacccgacct gtactcttgg ttggcattca
    14701 ccctgcaaaa aaaccaagaa ttgcgcgttc tgaaagctgc attgaacgaa ggccgtgatt
    14761 ctgttgccga agaactcgcc gccagccaag ctgctgccga ctcccgtgcc aacagcagcg
    14821 aaatccatcg tgcagacgtt gccaaacgcc tggccgattt gcctgccaac gcagaccaac
    14881 gcaaatctcc atttgccgac cgtatcaaag cgcaacaagc atggttgaac ctacctctgc
    14941 tgccgacgac caacatcggt tctttcccgc aaaccaccga aatccgccag gcacgctcag
    15001 ccttcaaaaa aggcgaactg tctgccgccg attacgaagc cgcgatgaaa aaagaaatcg
    15061 ccttggtggt tgaagagcaa gaaaaactgg acttggacgt actggtacac ggcgaagccg
    15121 agcgtaacga catggttgaa tacttcggcg aattgttgag cggttttgca ttcactcaat
    15181 acggctgggt acaaagctac ggctcacgct gcgtgaaacc accgattatc tttggcgacg
    15241 taagccgtcc tgaagccatg accgtggctt ggtctactta cgctcaaagc ctgaccaaac
    15301 gcccgatgaa aggtatgttg accggccctg taaccattct gcaatggtct ttcgtccgca
    15361 acgatattcc ccgcgctacc gtgtgcaaac aaatcgcact ggctctgaac gacgaagtat
    15421 tggatctgga aaaagcaggc atcaaagtca tccaaatcga cgaacctgcc atccgcgaag
    15481 gtctgccttt gaaacgtgcc gattgggatg cctacctgaa ctgggcgggc gaatctttcc
    15541 gcctgtcctc tgccggttgt gaagacagca ctcagatcca cacccatatg tgttactccg
    15601 agttcaacga catcttgccg gccatcgccg ctatggatgc tgacgtcatc accatcgaaa
    15661 cttcacgttc cgatatggaa ctcttgaccg cgttcggcga attcaaatac ccgaacgaca
    15721 tcggcccggg ggtttacgac atccacagcc cgcgcgtacc gactgaagcc gaagttgagc
    15781 acctgttgcg caaagccatc gaggttgtac cggttgagcg tctgtgggtg aatcctgact
    15841 gcggcctgaa aacacgtggc tggaaagaaa ctctggaaca actccaagtg atgatgaacg
    15901 taacccgaaa actgcgtgcc gaattggcga aataagccga gaccgtatga ataaataccg
    15961 tctgaaagcc tttcagacgg tattttgtcc tgatttgcgg cgcaagggcg cagttgccgg
    16021 aaaatctttt cattgcagct tgtttttttc tcattcggct ttatatgtgg gaaacaggca
    16081 aatcggagtt gtgtttgata gttttaaata atttatatta tttgaactat aaattataca
    16141 aatcattttg catggggtag aatgcccagc gattcacaat tatttctcaa accaatctat
    16201 taaggagctt aaaatggctt tgcaagatcg taccggtcaa aaagtacctt ccgtagtatt
    16261 ccgcacccgc gtcggcgaca cttggaaaga tgtgtctacc gatgatttgt tcaaaggcaa
    16321 aaaagtagtc gtattctccc tgcccggtgc atttaccccg acttgttctt cttcacacct
    16381 gccgcgttac aacgaattgt tcggcgcgtt caaagaaaac ggcgttgacg caatctactg
    16441 cgtatctgta aacgatacgt tcgtaatgaa cgcttgggct gccgaagaag aatccgacaa
    16501 catctacatg attcctgatg gcaacggcga atttaccgaa ggtatgggta tgctggtcgg
    16561 taaagaagac ttgggcttcg gtaaacgctc ttggcgttac tccatgctgg ttaacgacgg
    16621 cgtggttgaa aaaatgttca tcgaacctga agaaccgggc gatccgttca aagtatccga
    16681 tgcagatact atgctgcaat tcgttgctcc cgattggaag gctcaagagt ctgtggcaat
    16741 tttcactaaa ccaggttgcc aattctgcgc taaagccaaa caagctttgc aagacaaagg
    16801 tttgtcttac gaagaaatcg tattgggcaa agatgcaacc gttacttccg ttcgcgccat
    16861 taccggcaag atgactgccc ctcaagtctt catcggcggc aaatacatcg gcggcagcga
    16921 agatttggaa gcttacttgg ctaaaaactg atagctgttt gcttaaggcg gtttgattaa
    16981 actgtctgat ataccggata gagttattcg ggcggttctg cactgctgct ccgaataact
    17041 ctatatttat aagagaattt ggatattgtt gcactcaatc gaaattttgt ttttatttat
    17101 ctgaatgatg tttttgattg ggaaaatatt taaatgccgt ctgaaaccga tatgttctgt
    17161 gtcggcaatg tttcagacga aaacggaagg acaaagatta tgaaaaaaat tcaagcggat
    17221 gtcgtcgtac tcggcggcgg tactgccggt atgggtgcgt ttcgcaatgc ccgtttacat
    17281 tcggataatg tttacctgat tgaaaacaat gtgttcggca caacctgcgc gcgcgtgggt
    17341 tgtatgcctt ccaaactctt gattgccgcc gcagaagcgc gccaccacgc attgcatacc
    17401 gactcgttcg gcgtgcattt ggacaaagac agcatcgtcg tcaacggtga agaggtcatg
    17461 cagcgcgtta aatccgagcg tgaccgtttt gtcggctttg tcgttgccga tgtggaagag
    17521 tggcctgccg acaagcgcat catgggttcg gctaaattta tcgacgagca taccgtccaa
    17581 atcgacgagc atactcaaat tacggcaaaa agtttcgtga ttgctaccgg ttcgcgtccc
    17641 gtcatcctgc cgcaatggca gtctttgggc aatcgtttga ttatcaacga tgacgttttc
    17701 tcatgggata cgctgcctaa gcgcgttgcc gtgttcgggc cgggtgttat cggtttggaa
    17761 ctgggtcagg cattgcaccg tttgggcgtg aaagttgaaa ttttcggttt gggcggaatc
    17821 atcggcggca tttccgaccc cgtcgtttca gacgaggcga aagccgtgtt cggcgaagaa
    17881 ttgaaactgc atttggatgc taaaaccgag gtcaagctcg atgcagacgg caatgtagaa
    17941 gtccattggg agcaggatgg cgaaaaaggc gtatttgttg ccgaatatat gctggcagcc
    18001 gtgggccgcc gtccgaacgt tgacaatatc ggtttggaaa atatcaatat cgaaaaagat
    18061 gcgcgcggcg tacctgttgc tgatccgctg accatgcaga ccagtattcc gcatatcttc
    18121 atcgcaggcg atgcgtccaa ccaactgcct ctgctgcatg aagctgccga ccaaggcaag
    18181 attgccggcg ataacgcgag ccgctacccg aatatcggcg gcggtttgcg ccgcagcacc
    18241 atcggcgtgg tgtttaccag tccgcaaatc ggctttgtcg gtctgaaata cgcgcaggtt
    18301 gccgcgcaat accaagccga cgaatttgtc atcggcgaag tatcgttcaa aaaccaaggc
    18361 cgcagccgcg tgatgctggt aaacaaaggc catatgcgcc tgtatgccga aaaagccacc
    18421 ggccgcttta tcggcgcgga aatcgtaggc cctgccgccg aacatttggc gcacttgttg
    18481 gcatgggcgc atcaaatgaa gatgaccgtt ccgcaaatgc tggatatgcc gttctaccat
    18541 cccgttatcg aggaaggtct gcgtaccgcg ttgcgcgatg ccgatgcgaa attgaaagcc
    18601 tgaccgatat ggcaaaacaa tgccgtctga aattttttca gacggcattt tgtttttggg
    18661 gatggggtcg gatgctgata ccgtgtcggg aagggggcgg caaaactaaa aatctttctt
    18721 ttaatctgct gtttccacgc gtgtttgtca aaatctatca gtttgttttt aaaatacact
    18781 gttcaaaatg ggataaaaca ggtaaattaa cgtttatgta acccagtgta gcaatgggtt
    18841 tacggttttt gagtcgatat ataactacag aggaattgac tatgtctgcc aaaccgcgtc
    18901 ctgtttatct ggatttgccg aacatccgtc tgccgatacc cgggatagtt tccatccttc
    18961 accgcatcag tggggtcggg ctgtttatta tgctgccttt cctgctgtat ttcctgtccg
    19021 gtaccctgag tcaagagtct gcatttgaaa cttaccgtgc cattgtttcc catcctttgg
    19081 tcaagctggt tttaatcggt gtattgtggg cttatctgca ccattctctc gccggtatcc
    19141 gctttttatt tttggatgcg cacaaaggcc ttgagctgaa tactgcgcgc aataccgcta
    19201 aagccgtatt tgcttctgca ttggttttga ctgtcgtttt gggagcgttg ttatggtaga
    19261 acgtaaattg accggtgccc attacggttt gcgcgattgg gcgatgcaac gtgcgactgc
    19321 ggttattatg ttgatttata ccgttgcact tttagtggtt ctattttccc tgcctaaaga
    19381 atattcggca tggcaggcat tttttagtca aacttgggta aaagtattta cccaagtgag
    19441 cttcatcgcc gtattcttgc acgcttgggt gggtatccgc gatttgtgga tggactatat
    19501 caaacccttc ggcgtgcgtt tgtttttgca ggttgccacc atcgtctggc tggtcggctg
    19561 cttggtgtat tcaattaaag taatttgggg gtaagtatgg gttttcctgt tcgcaagttt
    19621 gatgccgtga ttgtcggcgg tggtggtgca ggtttacgcg cagccctcca attatccaaa
    19681 tccggtctga attgtgccgt tttgtctaaa gtgttcccga cccgttcgca taccgtagcg
    19741 gcgcagggcg gtatttccgc ctctttgggt aatgtgcagg aggaccgttg ggactggcac
    19801 atgtacgata ccgtgaaagg ttccgactgg ctgggcgacc aagatgcgat tgagtttatg
    19861 tgccgcgctg cgcctgaagc ggtgattgag ttggaacaca tgggtatgcc ttttgaccgt
    19921 gtggaaagcg gtaaaattta tcagcgtcct ttcggcggcc atactgccga acacggtaaa
    19981 cgcgcggtag aacgcgcctg tgcggttgcc gaccgtacag gtcatgcgat gctgcatact
    20041 ttgtaccaac aaaacgtccg tgccaatacg caattctttg tggaatggac ggcacaagat
    20101 ttgattcgtg atgaaaacgg cgatgtcgtc ggcgtaaccg ccatggaaat ggaaaccggc
    20161 gaagtttata ttttccacgc taaagctgtg atgtttgcta ccggcggcgg cggccgtatt
    20221 tatgcctctt ctaccaatgc ctatatgaat accggcgatg gtttgggtat ttgcgcgcgt
    20281 gcaggtatcc cgttggaaga catggaattc tggcaattcc acccgaccgg cgtggcaggt
    20341 gcgggcgtgt tgattaccga aggcgtacgc ggcgagggcg gtattctgtt gaatgccgac
    20401 ggcgaacgct ttatggaacg ctatgcgccg accgtaaaag acttggcttc tcgcgacgtt
    20461 gtttcccgcg cgatggcgat ggaaatctac gaaggtcgcg gctgcggtaa aaacaaagac
    20521 catgtcttac tgaaaatcga ccatatcggc gcagaaaaaa ttatggaaaa actgccgggc
    20581 atccgcgaga tttccattca gttcgccggt atcgatccga ttaaagaccc gattcccgtt
    20641 gtgccgacta cccactatat gatgggcggt attccgacca actaccatgg cgaagttgtc
    20701 gttcctcaag gcgacgaata cgaagtgcct gtaaaaggtc tgtatgcggc aggtgagtgc
    20761 gcttgtgctt ccgtacacgg tgcgaaccgc ttgggtacca actccctgtt ggacttggtg
    20821 gtattcggta aagctgccgg cgacagcatg attaaattca tcaaagagca aagcgactgg
    20881 aaacctttgc ctgcggatgc gggcgagctg acccgccaac gtatcgagcg tttggacaat
    20941 caaactgatg gcgaaaacgt tgatgcattg cgtcgcgaat tgcaacgctc cgtacaactg
    21001 cacgccggcg tgttccgtac tgatgagatt ctgagcaaag gcgttcgaga agtcatggcg
    21061 attgccgagc gtgtgaaacg taccgaaatc aaagacaaga gcaaagtgtg gaataccgcg
    21121 cgtatcgagg ctttggaatt ggataaccta attgaagtgg cgaaagcgac tttggtgtct
    21181 gccgaagcac gtaaagaatc acgcggtgcg cacgcttcag acgaccatcc tgagcgcgat
    21241 gatgaaaact ggatgaaaca tacgctgtac cattcagata ccaatacttt gtcttacaaa
    21301 ccggtacata ccaagccttt gagcgtggaa tacatcaaac cggccaagcg cgtttattga
    21361 tgcgttttca gacggttttc gcctcataag ctcgtctgaa atctaaccat acccacattg
    21421 aactgcttga atttataata caaaatcatt gggcagttga tgagaaaagg aacacttctc
    21481 atggaaaaaa tgagttttga aatttaccgt tacaacccgg atgttgatgc caagccttat
    21541 atgcagcgtt acgagttgga attggaaccg accgacgtga aacttttgga tgctttggta
    21601 cgcctgaaag cacaagacga taccttgtct ttccgccgct cctgccgcga aggcatttgc
    21661 ggttctgacg gtatgaacat caacggcaaa aacggcttgg cgtgtttgac cgatctgcgt
    21721 ggcttgaaac agccggttaa aattcgtcct ctgccaggtc tgcctgttat ccgtgacctg
    21781 attgtggata tgacccagtt cttcaaacaa taccattccg tcaaacctta tgttgtcaac
    21841 gataatccga ttgatgcgga caaagagcgt ctgcaaactc aggaagagcg taaagagttg
    21901 gacggtttgt acgagtgtat tttgtgcgcc tgctgttcga ctgcctgccc gtcattttgg
    21961 tggaaccctg ataaattcgt tggtccgtcc ggtttgctga atgcttaccg cttcattgcg
    22021 gacagccgag ataccatcac taatgagcgt ttggataatc tgaacgaccc ataccgtttg
    22081 ttccgttgcc acaccattat gaactgcgta gacgtatgtc ccaaacactt gaatccgacc
    22141 cgagccatcg gtaagattaa agagattatg ttgaaacggg ccgtttaaga aatgatggtt
    22201 tttgacgata ttgccaaacg gaaaatccgt tttcaaaccc gccggggatt gttggagtta
    22261 gatttaatct tcggcaggtt tatggaaaaa gaattcgagc atttgagcga taaagagctg
    22321 tccgagtttt ccgaaatcct tgaatttcaa gatcaagaat tgcttgcctt gattaacggg
    22381 cattcggaaa cggacaaagg gcaccttatc ccgatgcttg aaaaaatcag acgggcatga
    22441 ggcatctgaa atctgcctgc aggcagattt caaaatgcaa aagccgtctg aaggcaaaga
    22501 acgtgctgcg gatgcagtaa cgtgggttat aacttgcaaa ggagcaataa tatgtccaaa
    22561 tcaatcaaac tcaacgtacc gggtcaggca ggtttggagc tgccggtatt ggaagccagc
    22621 atcgggcacg atgtggttga cattcggggg ctgacaaaaa atacaggttt gttttccttc
    22681 gaccccggat ttgtttcaac cgcaagctgt gagtctaaaa ttacttacat cgacggcgat
    22741 caaggcttgc tttattatcg cggatacccc atcgagcagc tggccgaaaa gtccgattat
    22801 ttggaagtct gctacctgtt gatttacggc gaactgccga ctcccgagca aaaggcagaa
    22861 tttgacaata ccgtccgccg ccacacgatg gtgcatgaac agctgacttg gttcttccgg
    22921 ggcttccgcc gcgacgcgca tccgatggcg atgatggtcg gcgtggtcgg cgcactgtct
    22981 gcgttctacc aagacagctt ggacattagc aatcccgaac accgcaaaat cgcgatttac
    23041 cgcctgattt ctaaaatccc gaccattgcg gcaatgtgct accgctattc aaacggtctg
    23101 ccgttcaatt atccgaagaa taatctttct tattccgaaa acttccttca tatgatgttc
    23161 gccacgccgt gtgaagacta caaacccaat cccgttttgg cacgcgcgct cgaccgcatc
    23221 tttattttgc atgccgacca cgagcaaaac gcctcaactt caaccgtccg tctggcaggc
    23281 tcttcgggtg cgaatccgtt tgcctgtatt gctgccggta tcgcctgcct gtggggtgct
    23341 tcgcacggcg gtgcgaacga agccgtgttg aaaatgttgg acgaaatcgg cgatgtgtct
    23401 aatgttgccg catacatgga aggtgtgaaa caacgcaaat accgtctgat gggcttcggt
    23461 caccgcgtgt accgcaatat ggatccgcgt gccagcatta tgcgcgaaac ctgctatgaa
    23521 gttttgaagg aattgggctt ggaagacagt ccgaaattca aactggcgat ggaattggaa
    23581 cagattgcgc tgaaagaccc gttctttatc gaacgcaaac tgtatccaaa cgtcgatttc
    23641 tattccggca tcgtcctgtc cgcgctgggc atcccgaccg aaatgtttac cgtcatcttc
    23701 gccctgtcgc gcagcgtggg ctggatttcg cactggcacg agatgattag cgatccttcg
    23761 ctgaaaatcg gccgcccgcg ccagctttat accggttcgg aacgccgcga ttatgtgccg
    23821 ccaggcgaga ggtaacatac attaatatat tgtcaaacag gcaatatcag agaaccggat
    23881 tgtttcccga atccgtctga ttgtagtcgg atgaaatcaa gacaagcaat ccggtttaaa
    23941 atagggtaga ataaaatgtc ttttcagacg gcatcagttt agccgtcagg acgcggactt
    24001 ctaccctttg tttatatttt aaagaaaaga gcgcacgcca tgatggacga aaaactcaat
    24061 ttctcttacc tgttcggttc aaacgcacct tacattgagg aattgtacga ggcttttttg
    24121 gaaaaccccg atgcggttga tgaaaaatgg aagcagtatt tcaccgattt gagcaaacag
    24181 ccggggacgg ttgctgtcga tgtcgcacac acaccgattc gcgaatcatt tgttactttg
    24241 gcgaaaaaga aaattgcatc tgccgttgcg ggcagtgcgg atgaggcaat gctgaaaaag
    24301 caagtcagcg ttttacggct gatttctgct tatcgtatcc aaggcgtggg tgcagccaaa
    24361 cttgatccgc tcaaacgtat cccgccgcgc gatattgaag ccctcgatcc gaaattccac
    24421 ggtctgtcag atgccgatat ggcgctccaa ttcaatatgg gcgagggcga ttttgccaat
    24481 cggggcaaac tgcctttatc ccaaatcatc agcaacctca aacaaaccta ctgcggtcac
    24541 atcgcattgg aatatatcta tattcccaat accgaagagc gccgttgggt acgcaattat
    24601 tttgaaagcg tattgtccac gccgcactac aatgccgatc aaaaacgccg tattctgaaa
    24661 gaaatgaccg ctgccgaaac tttggaacgt tatctgcata ccaaatacgt cggtcagaaa
    24721 cgtttcggtg tcgaaggtgg cgaaagcgcg attgccggtt tgaactacct gattcaaaac
    24781 gccggtaaag acggtgtgga agaagttatc atcggtatgg cgcaccgtgg ccgtctgaat
    24841 gttttggtaa acattttggg caaaaaaccc ggcgatttgt tcgccgaatt tgagggccgt
    24901 gccgaaatca aactgcccag cggcgatgtg aaataccata tgggcttcag ctccgacatc
    24961 gctacgcctc acggtccgat gcacgtttct ttggcgttca acccgtccca cttggaaatc
    25021 gttaacccgg tagtcgaagg ttctgcacgc gccaagcaga aacgtttggg cgaaaacggc
    25081 cgcgataaag tcttgcccgt attgattcac ggcgactccg catttatcgg tttgggtgtc
    25141 aaccaagcga cattcaatct gtctaaaacg cgcggttata ccaccggcgg tacggttcac
    25201 atcgtgatca acaaccaaat cggctttacc acttccgata tccgcgatac ccgttcaacc
    25261 gtacactgta ccgatatcgc gaaaatggtt tctgctccgg ttatccacgt gaacggcgat
    25321 gatcctgagc gcgtttgctt tgccatccaa gccgctttgg attaccgcaa aaaattccat
    25381 aaagacatcg tgatcgacgt tgtctgctac cgtaaatggg gccacaacga gggcgacgat
    25441 ccgaccttga cccaaccgat gatgtacaaa aaagtatcgc aacacccggg tgcgcgtgct
    25501 ttgtacaccg agcaactgat tgccgaaggc gtggtaaccc aagccgaggc tgacggttac
    25561 atccaagcct accgtgatgc tttggataaa ggcgaacacg tcgagcaaac aacgttgagc
    25621 aacttccaac gcacacaaat tgactggagc aaataccaag gcaaagattg gcgcgaacac
    25681 atcgaaaccg gtttgcctgc cgcagatatt gagcgtctca ctgagaagtt taccgccgta
    25741 ccggaaggct ttgccctgca tccgactgca aaacgtgtga ttgaagcgcg taaagccatg
    25801 gcatccggca aacaggccat agattggggt atggccgaaa ccttggcata cgccagcctc
    25861 gtaaccaaag gtcatggcgt gcgtatctcc ggtgaggact ccggccgcgg tacgttctcg
    25921 caccgccacg ccgtattgca cgatcaaaaa cgcgaaaaat gggacgacgg tacttatgtt
    25981 cctctgcgcc atatgggcga aggaatgggc gagttcctgg ttatcgactc cattttgaac
    26041 gaagaggccg tgatggcgtt cgagtacggc tttgcctgct ccgcacctga caagctgacc
    26101 atttgggaag cgcaattcgg cgatttcgcc aacggcgcgc aagtgacgat tgatcaattc
    26161 ctgtcttcag gcgaaaccaa atggggtcgc ttgtgcggtc tgaccaccat cctgccgcac
    26221 ggttacgacg gtcaaggccc tgagcactct tctgcacgcg tagaacgttg gttgcaactg
    26281 tgttctgaga acaatatgca agtcatcatg ccatctgaag cgtcgcaaat gttccacctc
    26341 ttgcaacgtc aagtcttagg ttcataccgc aaaccgctag tgattttcat gtccaaacgc
    26401 ctgttgcgat tcaaaggggc aatgagcccg ctggaaaact tcaccgaagg ttcgactttc
    26461 cgtccggtta tcggcgatac cgccgaacgc gcaagcaacg acagcgtgaa acgcgtggta
    26521 ttgtgtgccg gtcaggttta ctatgacttg gaagccggtc gagccgaacg taaactggaa
    26581 gatgatgttg cgattgtccg cgttgagcag ctgtatccgt tcccatacga tgaggttaaa
    26641 gccgaactgg cgaaatatcc gaacgcaaaa tctgtggttt gggcgcaaga agagccgaaa
    26701 aaccaaggcg cgttctacca aatccgccac cgcatcgaag acgttatcag cgaagagcaa
    26761 aaactgtctt atgctggtcg tccaagcagc gcatcgcctg cagtgggcta ctcaagcaaa
    26821 cacattgctc aattgaaaca attggttgaa gacgctttgg cattgtaagc caagtagcat
    26881 tccgtctgag gctgctcagg cggaacaccc atatgcagaa tgaaaaacac acaacaggcc
    26941 gtctgaaagg gccattggag acacaaaatg attattgatg taaaagtacc tatgctgtct
    27001 gaaagcgtat ctgaaggcac gctcttggaa tggaagaaaa aagttggcga agcagttgcc
    27061 cgtgacgaaa tcctgatcga tatcgaaacg gacaaagtgg ttttggaagt accttctcca
    27121 caagccggcg tattggttga aatcgtagcg caagacggtg aaaccgttgt tgccgaccaa
    27181 gttttggcac gtatcgatac cgctgctact gccgctgctg aagcacctgc cgctgctact
    27241 gccgctgctg aagcacctgc cgccgctcct gccgaagctg ccccggccgc cgctcctgct
    27301 gctgcacaaa acaacgccgc tatgcctgcc gccgccaaac tggctgcaga gagcggtgtt
    27361 gacgtgaacg cattgcaagg ttccggccgt gacggtcgcg tattgaaaga agacgtacaa
    27421 aatgccgcag ccaaacctgc cgcagccgct gctcctgccg ttgcacttcc tgccggcgca
    27481 cgtcctgaag aacgcgtacc gatgagccgc ctgcgtgccc gtgttgcaga acgcctcttg
    27541 gcttctcaac aagaaaacgc cattctgact acattcaacg aagtcaacat gaaaccaatc
    27601 atggacttgc gtgcgaagta caaagaaaaa ttcgagaaag aacacggcgt gaaactgggc
    27661 tttatgtcct tcttcgttaa agccgctgtt gcagccctga aaaaataccc ggttgtgaat
    27721 gcttctgttg acggcaaaga cattgtgtac cacggctact tcgacatcgg tatcgcgatt
    27781 ggcagcccac gcggtttggt tgtgccaatc ctgcgcgatg ccgaccaaat gagcattgcc
    27841 gacatcgaac aagcaattgt tgattacgcg aaaaaagcca aagacggcaa aatcgctatc
    27901 gaagatctga ccggcggtac attcagtatt accaacggcg gtactttcgg ttctatgatg
    27961 tccaccccga tcatcaatcc gcctcaatct gcgattttgg gtatgcacgc cactaaagag
    28021 cgcgctgtgg ttgaaaacgg ccaagttgtt gtccgtccaa tgatgtatct ggctctgtct
    28081 tacgaccacc gtatcattga cggccgcgaa gctgtattga ccttggtagc cattaaagac
    28141 gcgttggaag acccagcccg cctgttgttg gatctgtaat cgtttcagac ggccttttat
    28201 ttgttaatga aaaggccgtc tgaattttta tagtggatta aatttaaacc agtacggcgt
    28261 tgcctcgcct tgccgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgatttaa
    28321 atttaatcca ctatatttag atgtagcgta atgtagtatc gtgctacaat aggctcaacg
    28381 aacgattgag gccgtctgaa acatttgatt cgaatgaatc ggcagatatg gactttcaga
    28441 cggctttttc ttaaaaccat caaaacgcag tcattcaaaa taaaaaagaa acgaaaagta
    28501 tcgtttttat tttgagatac tgctaaaagc aaaggatgac acgatgtctc aatatgatgt
    28561 agtagtgatt ggtgcaggcc cgggcggata cgttgccgcc atccgtgccg cacaactggg
    28621 tttcaaaact gcctgtgtcg atgcaggcgt taacaaagca ggcaatgccc ctgcattggg
    28681 cggtacttgc ttgaacgtag gctgtatccc ttctaaagcc ctgttgcaat ccagcgaaca
    28741 tttccacgca gcgcaacacg agtttgccga acacggtatc actgtcggcg acgtaaaatt
    28801 cgacgtggcc aaaatgattg agcgcaaaga tgccatcgta accaaactga ccggcggcgt
    28861 gaaattcctg ttccagaaaa acaaagtaac cagcctgttc ggtacggctt cctttgccgg
    28921 caaaaatagc gatgcttacc aaatcgaagt cgataacaaa ggtgagaaaa ccgttatcga
    28981 agccaaacac gtcatcgtag ccaccggttc cgtaccgcgt ccgctgccac aagtcgctat
    29041 cgacaatgtg aacgtattgg acaacgaagg cgcattgaac ctgaccgaag tacctgccaa
    29101 actcggcgtg atcggttccg gtgtgattgg tttggaaatg ggttccgtat ggaaccgcgt
    29161 gggtgcggaa gttaccattc ttgaagccgc gccgaccttc ctggctgccg ccgaccaaca
    29221 aatcgccaaa gaagccttca aatacttcac caaagagcaa ggtctgagca tcgaattggg
    29281 cgtgaaaatc ggcgacatca agtctgaagg caaaggtgtt tccgttgctt acgaaactgc
    29341 tgccggcgaa gccaaaaccg aagtattcga caaactgatc gttgccatcg gccgtattcc
    29401 aaacaccaaa ggcctgaacg ctgaagccgt cggcttggaa aaagacgagc gcggctttat
    29461 caaagtagat ggcgaatgcc gcaccaacct gcctaatgta tgggcaatcg gcgacgtggt
    29521 tcgcggcccg atgttggcac acaaagccag cgacgaaggc gttgccgttg ccgagcgcat
    29581 tgccggtcaa aaaccgcata tcgacttcaa caacgtaccg ttcgtgattt acaccgatcc
    29641 tgaaatcgct tgggtgggta aaaccgaaga gcagctcaaa gccgaaggcg tggagtacaa
    29701 aaaaggtact tcaggttttg gtgcgaatgg tcgcgcattg gcaatgggca aagccaaagg
    29761 tacggttaaa gtgttggcag acgccaaaac cgaccgcatc ttgggcgtac acatgattgg
    29821 tccggttgtc agcgaattgc ttaccgaagg cgtgactgcg ctcgaattct tcgccagcag
    29881 cgaagacatc gcccgcatta tccatgccca cccaaccttg tccgaagtgg ttcacgaagc
    29941 tgcattggcg gccgacaaac gcgctttgca cggttgatag acattaaggc ctccgacaaa
    30001 ttgaatgttc cgagagcttc gttttctgat ttataattcc gtcagacaaa caaacagcat
    30061 ttacattcat tatgaacaaa gaaatagtcg gtattttctt tataccggcg ggcatcatca
    30121 gcatgtgtat ggccgcattg tggcagatgt atgtgatgat gaccgaaact tatacgctca
    30181 accgtttcaa agataaagaa ttggtttggc gcgtggcatt gttgtttatc agtttcagcc
    30241 ttgccgttta tctgctctgt ccgaattcgc gtaaaaaagg catcgtcttt tttattctcg
    30301 ggggaggcgg tgcagccatg tatctgctgg cgcggatgtg gttgcctttc agcaagtgaa
    30361 acgacgattt tccgaccgcc gaaaggtagt ctgaaacgca cgggcttgcc atttggaggc
    30421 aaactcgggg cattccacta atctaaagga gaaatccatg aatttacacg agtatcaggc
    30481 taaagaactg ctggctagct acggtttgcc cgtacaaggc ggtattttgg cacacaacgg
    30541 cgaagaagcc gctgcagctt acgacaaatt gggcggcaaa ttcgctgttg tcaaagcaca
    30601 agtacacgcc ggcggccgcg gtaaagcggg cggcgtaaaa gtcgttaaaa gccgcgaaga
    30661 agctaaagaa gtggctgaaa gcctgattgg caccaacttg gtaacttacc aaactgatgc
    30721 caacggccaa cctgtcaaca gcgttttggt ttgcgaagac atgtatcctg ttcaaaccga
    30781 gctgtacttg ggcgcagtgg ttgaccgttc tacccgccgc gttacattca tggcttctac
    30841 cgaaggtggc gtagaaatcg aaaaagttgc tgccgaaact ccagaaaaaa tcttcaaagt
    30901 aaccgttgat ccgctggtcg gcctgcaacc ttgccaagct cgcgaagttg ccttccaact
    30961 gggcttgaaa gacaaacaaa tcaacgagtt cgtcaaactg atgaccggcg cgtacaaagc
    31021 gtttgtcgaa aacgacttcg ccctgtttga agtcaacccg ctggcagttc gcgaaaacgg
    31081 cgcactggct tgcgtggacg gcaaaatcgg catcgacagc aacgcgctct accgcctgcc
    31141 aaaaatcgct gaattgcgcg acaaatctca agaaaacgaa cgtgaactga aagcttctga
    31201 attcgacctg aactatgttg ccctggaagg caacatcggc tgtatggtta acggtgccgg
    31261 tttggcgatg gccaccatgg acatcatcaa actcaaaggc ggtcaacctg ccaacttctt
    31321 ggacgttggc ggcggcgcaa ccaaagaccg cgtggttgaa gcgttcaaac tgattttgga
    31381 agacaaatcc gttaaaggcg tattgatcaa catcttcggc ggtatcgtac gttgcgacat
    31441 gattgcggaa gccatcgtgg cagccgttaa agaaatcaac gtcgacgttc ctgtcgttgt
    31501 tcgtttggaa ggtaacaacg ccgaactcgg cgcgaaaatc ctgaacgaat caggcctgaa
    31561 actgacttct gcagacggcc tgaatgacgc agccgaaaaa attgttgcag ccgtaaacgc
    31621 ctaaggagaa aagaatgagc gtattgatta ataaagacac caaagtattg gttcaaggtt
    31681 tcaccggtaa aaacggtact ttccactccg aacaagctct ggcttacggc actaaagttg
    31741 tcggcggcgt taccccgggc aaaggcggtc aaacccacct ggatctgcct gtattcaaca
    31801 ccatgaaaga agcggttaaa gaaaccggcg cggatgcatc tgtaatttac gttcccgctc
    31861 cgtttgtgtt ggattctatc gttgaagccg ttgattcagg cgtaggcttg gtcgttgtga
    31921 ttaccgaagg cgtaccgact ctggacatgc tcaaagccaa acgttatttg gaaaccaacg
    31981 gcaacggcac acgtttggtg ggccctaact gcccgggcgt gattacccca ggcgagtgca
    32041 aaatcggtat tatgccgggc cacatccaca ctccgggccg catcggtatt atttcccgtt
    32101 ccggtacatt gacttacgaa gccgtggcac aaaccaccaa actgggcttg ggtcaatcaa
    32161 cctgtatcgg tatcggcggc gacccgattc ctggtatgaa ccaaatcgac gcactgaaac
    32221 ttttccaaga agacccagat accgacgcca tcatcatgat cggtgaaatc ggcggtactg
    32281 cggaagaaga agcagccgaa tacatccaat ccaacgtaag caaacctgtt gtcggctaca
    32341 tcgccggtgt taccgctcct aaaggcaaac gcatgggcca cgccggtgcg attatttccg
    32401 gcggcaaagg tactgccgag gaaaaattcg ccgcgtttga aaaagccggt atcgcttaca
    32461 cccgcagccc tgccgagttg ggcactacca tgctggaagt gttgaaaaca aaaggtttgg
    32521 cataatcagg tttgacaact gattgaacat caaatgccgt ctgaaaccgg aaatcgggtt
    32581 tcagacggca ttttgtttgt catttcaaaa agaggcagcc tcaacatacc cacattattt
    32641 ttgccctttg aggcaggcag aatatttatc ggtcaaacag gagcaaaccc gtaagtcagg
    32701 ttgcacacgc cttgtttgaa gttggggaat gataactatc gcatttgaaa agactgatat
    32761 tattttttgc aggattctgt tttagcacgc gacatttttt gtttgatagg attggaatgg
    32821 atgtagtccg cgcaacgctg caaatccgtt tcgtcgcgca cggtgtgtcc gtaaaaacgc
    32881 cgttgccaaa taccgcgttc gtgctgccgc tgtttgccgg cggataagtt tttagtataa
    32941 ggggaatatg cggagaattt ggttttaatc agccgccgga gtagggaata atccgcatcg
    33001 tcgggcggca gcgtccaaat ggcgtgaata tggttcggca gcacgcatac ggcgacggtt
    33061 tcaaagggat attgtttttg cacatccata taagccgcac gcgaagccga tatgttcgac
    33121 aagcaggcgc gattttggat cggcgagttt gacggtgaaa aagaatgtgc cgccggcaat
    33181 gaagtttctg cgataacgcg ccataggatt tttgtttgtg gtttgggtgt tggagaagga
    33241 atggtttgtt gtttaaggtt ttaactttgt tggagctgat gttttcagac gacattggag
    33301 gccgtctgaa agcgctggct tcaacgaagt taaaagttgg aacggcaaac cgttaatcaa
    33361 ccaaccgcgt acgtaccgca cacacactgc acgttggctt tgaagtttcg tagactcaca
    33421 ggtaggtgtg gcgcaaccac gcatacggtg ggttgggtat aaaggctaca acttgctata
    33481 gcttgcttgc tattcaaaat aagaatgctt aattgctgtt gctaattgag cagcaaggat
    33541 gggaggaacg gcattaccaa cctgcgtgaa ttgccgcgca accgctcctg taaaaatata
    33601 atcatcagga aacgtttgta atcgtgcact ttcacgaaca gataacgttc ttgggatata
    33661 tggatgtaag tgagaccttc ctccaccttt agtgccacca gcaataatag ttttagatgg
    33721 tttgcttgga tctaacctat ctacacgtcc ctttttatca cgttgcccat aagctaattc
    33781 catatagcgt aaaatcgatt cagccgaatg ttctcttgta atatggttat gttggttaat
    33841 aattggaatt tcaaatgtat taccacatgc caatggggta ttggttggtt gtagcggact
    33901 aaatttttta tctcctaaat aaccaattat aaatagccta attctctgtt gaggtacatg
    33961 aaaatattca gaattaatta tttgaggttt atagagtgag tagccttgct gctgtaaatc
    34021 acaatataca ctagctaact gtttaccatt atctatatcc atcaatccag agacattttc
    34081 aataagaaat actttaggac gaaaaatctt aatcaattca acgtaattaa ataatagatt
    34141 accattttgc ttatgctcaa agccgacacg tttaaaattt ttatcagttt ttgagaatct
    34201 ctggtttgag gctatggaga aaggctgaca gggaggacct ccaataaata caccatcaaa
    34261 acttaaagac ccaatattat ttctgattag ttcaaccatt tcttctgttt cagaaacatc
    34321 agcattaaac acagaccaat tagggcgatt aagacgcaat gtatcacaaa ataaaggtat
    34381 tttttcaatc agcatagaat gttgaaagcc tgctgcttca aaccctaagt ctaatccacc
    34441 ggctcctgaa aaaaaactaa ttatcggaat gtgattttcc tttttttgaa aatcacttag
    34501 aaccaaattc tttctctttt caaaaggctt tttatcatga tgaatataaa ttaaatcatc
    34561 atagctcatt tgattctggc gtattagttc attcaatgga ataccttgta ataaactttg
    34621 ttctaataat tttctttgtg aaataattct attattttct tggtcaaata aagaggtttg
    34681 cggtattaac atagatactc ctatctattt ataaattctt ttaactttac ttttagcttc
    34741 ttcatataca tgatatgctt cttcataact aacaaaatct tttttcttgt gcaaattaag
    34801 ccaaaaagta tagtacttgt gtgtggcacg atgacaggtt ggacagatac caaccaaatc
    34861 ttttaatgaa gtagttttat cttctaccct tacaggagaa gatagtggta atagatggtg
    34921 tacttcaatg atatgttcgc tccattcata gctcctttgc gtatctaatc cacacatgcg
    34981 acatatttgt gcattttggg tcttgctaaa atagattttt cttaaaatac tattacgttc
    35041 tgtaattaaa tgggtgactt taattttacc accctcacta aacgcctgat catcaataga
    35101 taatgaaacg atttcattca tcaataagaa attattattt gtcttaccca atctcattat
    35161 ctctaaacta tctaatcttt cttgtggttg tgtatctgca ataaattggt tttctaataa
    35221 atgtagttca ctaaaatctg taatatctaa aaacaactta ttttcatacc aagacaaaaa
    35281 agagaattga ctaataaact ttaccaactc tctaatttga cgaggatcat atttaattgc
    35341 ttctttattc ataattaact gtgagaatga aaaagaagca atacctgtaa tactattatt
    35401 actagcgatt ttgtaaataa tttcatccat gtccaaaaaa ttaactcctg tttccttata
    35461 ttttgaaatt aaagctctaa taattgccaa aacagggaaa acccgatttt cagatggttg
    35521 atatcctgaa aaagctggtg atgggtagga aaaatgtgtt gcaaaatgta gtaaataatc
    35581 atcgcaacta atttcatcat tctgaactaa atcggcacat aaatctgtag tacagattaa
    35641 cccattatgc tccgttgcga gtaacaaaca tttaaaaaca cgaccataat tacgccaaac
    35701 tggatattgt gaactgttag gggcaaaagg taagtttgtc tcttgagtca ggcttaatct
    35761 tacaatatca atatcttttg cacgtggctt tggacattga ttaattccat ataatccttt
    35821 tgcaattttt ttaatttcat caaatctaaa ataatctaat ctaccttgat caaaacgcca
    35881 ttcaatcatt tttaacctct atgaaatatc atttttcagt aatctgaaaa ttatcctaaa
    35941 accaccttca aatacttccc agtataactc cccttaacct tcgccacttc ctcagggcta
    36001 ccttgggcaa taatcctccc cccgctatcg ccaccttccg gtcctaaatc tacaatccaa
    36061 tccgcagttt taatcacatc cagattatgc tcaataatca caatcgagtt gcctttgcct
    36121 ttcagacggc ctatgacttc cagcagcagg gcgatgtcgg cgaagtgcag gccggtggtg
    36181 ggttcgtcga ggatgtagag cgttctgccg gtgtcgcgtt tggagagttc caaggcgagt
    36241 ttgacgcgct gggcttcgcc gccggagagg gtggtggcgg actggccgag gcggatgtag
    36301 ccgagaccta cgtccatcag ggtttgcagt ttgcgcgata cggtggggac ggcgtcgaaa
    36361 aattcgcggg cttcttcgac ggtcatgtcg aggacttggc tgatgttttt gcctttgtat
    36421 tggatttcga gggtttcgcg gttgtagcgt ttgccgtggc agacttcgca ggggacgtac
    36481 acgtcgggca ggaagtgcat ttcgacttta atcacgccgt cgccttggca ggcttcgcag
    36541 cggccgcctt tgacattgaa ggagaatctg ccgacgttgt agccgcgttc gcgcgagagg
    36601 gggacgccgg cgaagagttc gcggatgggg gtgaacaggc cggtgtaggt ggcggggttg
    36661 gagcgcgggg tgcggccgat gggggattgg tcgacgttga tgactttgtc gaggtgttcg
    36721 aggccgcgga tgtcgtcgta tggggcgggt tcttcttggg cgcggttgag ttcgcgggcg
    36781 gtgattttgg cgagggtgtc gttaatcagg gtggatttgc cgctgccgga tacgccggtg
    36841 atgcaggtaa tcaaaccgag cggcagttcg agggtaacgt ttttgaggtt gttgccgcgc
    36901 gcgcctttga ggacgagcat ccggtcggga ttgacgggtg tgcgttcaga cggcacggca
    36961 atggattttt tgccgctgag gtattgtccg gtaatggagt tttcgcattt ggcgacgttt
    37021 tcgggtgtgt cggcaatcag tacgttgccg ccgtgttcgc cggcgcccgg gcccatatcg
    37081 acgacgaaat cggcttcgcg gatggcatct tcgtcgtgtt cgaccacaat cacgctgttg
    37141 cctaaatcgc gcaggcgttt gagggtggcg agcaggcggt cgttgtcgcg ctggtgcagg
    37201 ccgatggagg gttcgtccaa aacgtacatt acgccggtca ggccgctgcc gatttggctg
    37261 gcgaggcgga tgcgctgggc ttcgccgccg gaaagggttt cggcggaacg ggagagattc
    37321 aggtaatcca gcccgacatt aatcaaaaag ccgagacgtt cggtgatttc tttgaggatt
    37381 ttttcggcga tttgtttttt gttgccgtct aaatccagtg tttcaaagaa ttggtgggtt
    37441 ttggtgagcg gccaggcgga aacttcgtgc aacggctcac cgctgacgta aacgtagcgg
    37501 gcttctttgc gcaaacgtgc gccgccgcag ctcgggcagg cgcggtggtt ttggtattcg
    37561 cgcagttttt cgcgcacggt ttcgctgtcg gtttcgcggt agcggcgttc gagattgggg
    37621 atgatgcctt cgaaggcgtg gctgcggttg aaggtggtgc cgcgttcgga caggtaagtg
    37681 aaatcaatga cttccttgcc cgagccgtgc aacacgactt ttttgatttt tgcaggtagg
    37741 gtttcccaag cagcctgcac gtcgaaaccg taatgccgcg ccagcgactg aatcatttgg
    37801 aaatagaact ggttgcgctt gtcccagccg tcaatcgcgc ccgaagccag cgacaattcg
    37861 ggatgcgcga ccactttttc ggagtcgaag aaattggtgt tgcccaagcc gtcgcaagtc
    37921 gggcaggagc ccatcgggtt gttgaacgaa aagaggcgcg gctccaattc gggcaggctg
    37981 tacgaacata cggggcaggc gaaacgcgcg gaaaaccaat gctcttcgcc gctgtccatt
    38041 tccatcgcca gcgcgcgctc gttgccgtgg cgcagcgcgg tttcaaaact ttccgccagc
    38101 cgctgcttga tgttcgcctt cactttcacg cggtcgatga ccacgtcgat attgtgtttg
    38161 atgttttttt ccagcttcgg cacttcgtca agctgataga cctcgccgtc cacgcgcaca
    38221 cgggcaaagc cttgcgcctg caagtcagca aagaagtcga caaactcgcc cttacgctca
    38281 cgaactgccg gcgcaagaat catcacgcgc gtgtcttccg gcagttttaa tacggcatcg
    38341 accatctgag atacggtttg gctggatagc ggcagattgt gttcggggca atacggcgtg
    38401 ccgacgcgtg cgtacaaaag gcgcaggtaa tcgtggattt ccgtaaccgt gccgacggtg
    38461 gaacgcggat tgtggctggt ggatttctgc tcgatggaaa tcgcgggcga cagaccctcg
    38521 atcaaatcga catcgggttt gtccatcatc tgcaaaaact gccgcgcata ggcggacagg
    38581 ctctcgacat aacgccgctg tccttcggca tacagcgtgt cgaacgccag cgatgatttg
    38641 ccgctgcctg acaatcctgt taccaccacc agcttgtggc gcggaatgtc taaatcgacg
    38701 tttttcaaat tatgcgtgcg cgcgccgcgt atgcggatgg tgtcgttgtc gtgcgaatgt
    38761 tggggatgat ggttgcacat aatggatgcc gcctgaaaaa taaaggaaaa ctggtattgt
    38821 agcactttct cggatgccgt ctgaagccgc gttcagacgg catttgtcgg cggagtacgg
    38881 cagattccgc tataatgtcg gcaattttaa cccgcttgaa caaaaggatg acaaatgaac
    38941 cgtctttacc cccacccgat tatcgcccgt gagggctggc cgattattgg cggcggtttg
    39001 gctttgagcc tgctggtgtc gatgtgttgc ggctggtggt ctttgccgtt ttgggtgttt
    39061 accgtatttg ccctgcagtt tttccgcgac cctgcgcgtg agattccgca aaatcctgaa
    39121 gcagtgttga gtccggttga cggccgtatc gtggtggtcg agcgcgcacg cgatccgtat
    39181 cgtgatgtcg atgctttgaa aatcagtatt tttatgaacg tgttcaacgt gcattcgcaa
    39241 aaatcgcctg ccgattgtac ggtaacgaaa gtggtctata acaaaggcaa attcgtgaat
    39301 gcggatttgg acaaagccag cacggaaaac gaacgtaatg cggtgttggc gactacggct
    39361 tccggtcgtg aaattacttt tgttcaagtg gccggtttgg tggcgcgccg tattttgtgc
    39421 tacacccaag caggtgcgaa actgtcccgc ggcgaacgtt atggctttat ccgcttcggt
    39481 tcgcgcgtgg atatgtatct gcctgtcgat gcgcaggcgc aagtggcgat tggcgataaa
    39541 gtaactggcg ttagcactgt attggcgcgt ttgccgctga ctgcgccgca aatcgaatcc
    39601 gagcctgagt ctgagcctgc tttacaaact gctccggttg aaacagcggc aaacccatct
    39661 gccgaacaac ggcaaatcga ggcagcggcg gctaagattc aggcggctgt gcaagatgtg
    39721 ttgaaagatt aattttgcga actgaaatag aaaatatcag taccatcatt cacacgaatg
    39781 aggaagtttg gttttttgaa tttttgctaa tgttcacacc gttatgttca cgaaaatggg
    39841 aatctggaaa tttaacgtta cggtgattta tcagaaatat agtggattaa atttaaacca
    39901 gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
    39961 ctgatttaaa tttaatccac tataacagaa actgaacgaa ttagattccc gcctgcgcgg
    40021 gaatgacaac tcattagtta cctaacactt aaaaaacaga aacctttccg cgtcattccc
    40081 acgaaagtgg gaatccggga acttaacgtt acagtgattt atcggaaacg actgaaaccg
    40141 aagagaccgg attcccgcct gcgtgggaat gacaactcat tagttactta aaacttaaaa
    40201 aacagaaacc tttacgcgtc attcccacga aagtgggaat ccgggaactt aacgttacag
    40261 tgatttatcg gaaacggctg aaaccgaacg aattggattc ccgcctgcgc gggaatgacg
    40321 actcattagt tacctaaaac ttaaaaaaaa cagaaacctt tccgcgtcat tcccatgaaa
    40381 gtgggaatct agaacccaaa tgctaaggcg atttatcgga aacggctgaa aacgaagaga
    40441 ctggattccc gcctgcgcgg gaatga
//
LOCUS       DAWHXS010000003        39665 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWHXS010000003 DAWHXS010000000
VERSION     DAWHXS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.003"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..2712
                     /gene="recB"
                     /locus_tag="WIE22_000089"
     CDS             <1..2712
                     /gene="recB"
                     /locus_tag="WIE22_000089"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ4126243.1"
                     /translation="LASDKLEAGLKKGKTPDAAVFAELQKLADFGRDLNALEEAEEAT
                     MIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAETLARAVAENWET
                     ALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIYAYLQAAEDARH
                     RYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESRLSPKRPAVQVR
                     WLHENDNEKANKDVLRRRAADYCADEIAHALNEAAECRLNFKGCPLQSGDIAVLVRTH
                     NEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPHRAGTLRFVLTSGI
                     FGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQTHGIETRLLSRN
                     NGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNRAIRLESDEDLV
                     KIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAKAQLSEDEQKQY
                     ADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQDSRETVRRAYA
                     CEKDGIAMLKRNWRRVADNAPSGTNFAFTEDAPPPAAYRGNAGQTAEFAANSIPERRF
                     RFVRHTSFTALSRHTQTPDGGEEDACPSLDAAEISVAAMPSETPTASDGISIHNFPKG
                     TQAGLCLHEILEDFKFGQAAAGQETLITDKLKKYGFEEIWLPAVAEMAEACRKTPLTG
                     AYGLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRAAAETLDFHTVN
                     GFLNGFIDMVCQDPEGNICVIDYKSNHLGADASAYTRQAMDEAVAHHHYYLQALIYAV
                     AAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            2751..3110
                     /locus_tag="WIE22_000090"
     CDS             2751..3110
                     /locus_tag="WIE22_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ4126244.1"
                     /translation="MNIKHLITTALIASAAFAAQAAPQKPLSAAQTAQHPVVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            3235..3714
                     /locus_tag="WIE22_000091"
     CDS             3235..3714
                     /locus_tag="WIE22_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ4126245.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTKTDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(3990..5369)
                     /gene="radA"
                     /locus_tag="WIE22_000092"
     CDS             complement(3990..5369)
                     /gene="radA"
                     /locus_tag="WIE22_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ4126246.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSTVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            5397..5558
                     /locus_tag="WIE22_000093"
     CDS             5397..5558
                     /locus_tag="WIE22_000093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126247.1"
                     /translation="MPSEIGTVGITPIRQHRRRHVLSIHGGYCRIALFFHPSASDGIS
                     GGFLPAYPP"
     gene            complement(5546..6610)
                     /locus_tag="WIE22_000094"
     CDS             complement(5546..6610)
                     /locus_tag="WIE22_000094"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ4126248.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(6685..7902)
                     /locus_tag="WIE22_000095"
     CDS             complement(6685..7902)
                     /locus_tag="WIE22_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ4126249.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            complement(7899..9233)
                     /locus_tag="WIE22_000096"
     CDS             complement(7899..9233)
                     /locus_tag="WIE22_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ4126250.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGNNALATCALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(9248..10009)
                     /locus_tag="WIE22_000097"
     CDS             complement(9248..10009)
                     /locus_tag="WIE22_000097"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ4126251.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(10027..10449)
                     /locus_tag="WIE22_000098"
     CDS             complement(10027..10449)
                     /locus_tag="WIE22_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126252.1"
                     /translation="MKKTFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            complement(10700..11020)
                     /locus_tag="WIE22_000099"
     CDS             complement(10700..11020)
                     /locus_tag="WIE22_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ4126253.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(11043..11669)
                     /gene="upp"
                     /locus_tag="WIE22_000100"
     CDS             complement(11043..11669)
                     /gene="upp"
                     /locus_tag="WIE22_000100"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ4126254.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(11775..12086)
                     /gene="grxD"
                     /locus_tag="WIE22_000101"
     CDS             complement(11775..12086)
                     /gene="grxD"
                     /locus_tag="WIE22_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ4126255.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(12187..12401)
                     /locus_tag="WIE22_000102"
                     /pseudo
     CDS             complement(12187..12401)
                     /locus_tag="WIE22_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
     gene            complement(12427..13206)
                     /locus_tag="WIE22_000103"
     CDS             complement(12427..13206)
                     /locus_tag="WIE22_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ4126256.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            complement(13211..13561)
                     /locus_tag="WIE22_000104"
     CDS             complement(13211..13561)
                     /locus_tag="WIE22_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ4126257.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(13565..14542)
                     /locus_tag="WIE22_000105"
     CDS             complement(13565..14542)
                     /locus_tag="WIE22_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ4126258.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(14576..15688)
                     /locus_tag="WIE22_000106"
     CDS             complement(14576..15688)
                     /locus_tag="WIE22_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ4126259.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            15874..16575
                     /locus_tag="WIE22_000107"
     CDS             15874..16575
                     /locus_tag="WIE22_000107"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ4126260.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELVGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            16717..18510
                     /gene="lepA"
                     /locus_tag="WIE22_000108"
     CDS             16717..18510
                     /gene="lepA"
                     /locus_tag="WIE22_000108"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ4126261.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            18655..19674
                     /gene="lepB"
                     /locus_tag="WIE22_000109"
     CDS             18655..19674
                     /gene="lepB"
                     /locus_tag="WIE22_000109"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ4126262.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFAAVFLIFVVLCGTAWAVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSDLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(19893..20042)
                     /locus_tag="WIE22_000110"
     CDS             complement(19893..20042)
                     /locus_tag="WIE22_000110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126263.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(20088..20993)
                     /locus_tag="WIE22_000111"
     CDS             complement(20088..20993)
                     /locus_tag="WIE22_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4126264.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGPYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFNERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            complement(21456..22388)
                     /gene="cysK"
                     /locus_tag="WIE22_000112"
     CDS             complement(21456..22388)
                     /gene="cysK"
                     /locus_tag="WIE22_000112"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ4126265.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(22582..23187)
                     /locus_tag="WIE22_000113"
     CDS             complement(22582..23187)
                     /locus_tag="WIE22_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126266.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(23184..24035)
                     /gene="dapF"
                     /locus_tag="WIE22_000114"
     CDS             complement(23184..24035)
                     /gene="dapF"
                     /locus_tag="WIE22_000114"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ4126267.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFIPEHAPIAAWADRFRGIG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAQVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            24173..24469
                     /locus_tag="WIE22_000115"
     CDS             24173..24469
                     /locus_tag="WIE22_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126268.1"
                     /translation="MFRILLLLFPCAILVACTRNIPANPTVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            24483..25151
                     /locus_tag="WIE22_000116"
     CDS             24483..25151
                     /locus_tag="WIE22_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ4126269.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(25224..27344)
                     /gene="pnp"
                     /locus_tag="WIE22_000117"
     CDS             complement(25224..27344)
                     /gene="pnp"
                     /locus_tag="WIE22_000117"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ4126270.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(27581..28444)
                     /locus_tag="WIE22_000118"
     CDS             complement(27581..28444)
                     /locus_tag="WIE22_000118"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ4126271.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            28643..29518
                     /gene="rfbD"
                     /locus_tag="WIE22_000119"
     CDS             28643..29518
                     /gene="rfbD"
                     /locus_tag="WIE22_000119"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ4126272.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLLSSGY"
     gene            complement(30234..30467)
                     /locus_tag="WIE22_000120"
     CDS             complement(30234..30467)
                     /locus_tag="WIE22_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ4126273.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(30633..30833)
                     /locus_tag="WIE22_000121"
     CDS             complement(30633..30833)
                     /locus_tag="WIE22_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ4126274.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(31005..31880)
                     /gene="xerD"
                     /locus_tag="WIE22_000122"
     CDS             complement(31005..31880)
                     /gene="xerD"
                     /locus_tag="WIE22_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ4126275.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSVCKRLYIWMEREGIRTDNPTCLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(31932..32372)
                     /locus_tag="WIE22_000123"
     CDS             complement(31932..32372)
                     /locus_tag="WIE22_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ4126276.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            32693..33631
                     /locus_tag="WIE22_000124"
     CDS             32693..33631
                     /locus_tag="WIE22_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ4126277.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            33760..34053
                     /gene="hfq"
                     /locus_tag="WIE22_000125"
     CDS             33760..34053
                     /gene="hfq"
                     /locus_tag="WIE22_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ4126278.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            34138..34704
                     /locus_tag="WIE22_000126"
     CDS             34138..34704
                     /locus_tag="WIE22_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4126279.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            34758..35132
                     /locus_tag="WIE22_000127"
     CDS             34758..35132
                     /locus_tag="WIE22_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ4126280.1"
                     /translation="MQYLIVKYSHQIFVTVTILLFNIRFFLLWKNPEKPLAGLWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSVKFYAV
                     YLLAICCVACIVYLAKTKVLPF"
     gene            35142..35636
                     /gene="folK"
                     /locus_tag="WIE22_000128"
     CDS             35142..35636
                     /gene="folK"
                     /locus_tag="WIE22_000128"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ4126281.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRERSFRNAPRTLDLDII
                     DFDGLSSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDK"
     gene            complement(35763..36242)
                     /locus_tag="WIE22_000129"
     CDS             complement(35763..36242)
                     /locus_tag="WIE22_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ4126282.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(36462..37199)
                     /gene="ubiE"
                     /locus_tag="WIE22_000130"
     CDS             complement(36462..37199)
                     /gene="ubiE"
                     /locus_tag="WIE22_000130"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ4126283.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(37242..37610)
                     /locus_tag="WIE22_000131"
     CDS             complement(37242..37610)
                     /locus_tag="WIE22_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ4126284.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            37730..39349
                     /gene="mnmC"
                     /locus_tag="WIE22_000132"
     CDS             37730..39349
                     /gene="mnmC"
                     /locus_tag="WIE22_000132"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ4126285.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITPAEAEKIAGIPLNTPYAEPLCGLFWQYGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(39346..39495)
                     /locus_tag="WIE22_000133"
     CDS             complement(39346..39495)
                     /locus_tag="WIE22_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126286.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         9630 a        11419 c         9477 g         9139 t
ORIGIN      
        1 cttgcatccg acaaactcga agccggactg aaaaaaggca aaacgcccga tgcggcagta
       61 tttgccgaat tgcagaaact ggcagacttc gggcgcgatt tgaacgcact cgaagaagcg
      121 gaagaagcaa caatgatccg gctgcaactg gatttaatcg aatacctcaa ccgcagcctt
      181 gccgagatga aaaaatcgcg ccgcgaacgc ggtttcgacg acctgctgct cgatgtccac
      241 accgcgctga ccgacaatcc gcacgccgaa accctcgccc gcgccgttgc cgaaaactgg
      301 gaaaccgcgc tgatcgacga gttccaagac accgacccgc tgcaatacga aatcttccaa
      361 aaaatcttca tcgcccaaaa ccgaccgctg ttcctcgtcg gcgatcccaa acaggcgatt
      421 tacagctttc gcggagcgga catttacgcc taccttcagg cggcggaaga cgcgcggcac
      481 cgctacacgc tcgccaccaa ctaccgcagc cacgccgcgc ttatcggcag cataggcgcg
      541 ctgttccgcc tcaaagaacg cccgttcgtt ttggaaaaca tcggctattc ggaagtcggt
      601 gcggcgcgtg ccgaaagcag attgtcgccc aaacgtcctg ccgtacaggt tcgttggctg
      661 cacgaaaacg acaatgaaaa agccaacaaa gacgttttgc gccgccgtgc cgccgactat
      721 tgcgccgacg aaatcgccca cgcgctcaac gaagccgccg aatgccgtct gaatttcaaa
      781 ggctgcccgt tgcagtcggg cgatattgcc gtgctggtac gcacgcacaa cgaggcggtg
      841 atggtttccg ccgccctgaa aaaacggcag gtgcaaagcg tcctgctttc gcgcgaatcc
      901 gtgttcgcct cgcccgaagc cgccgccctg tccgcactca tcggcttctg gctcgaaccg
      961 caccgcgccg gaacgctgcg ctttgtccta acaagcggca tattcggtta tgacgcgcag
     1021 caattgcacg acttcaacca aaacgaaagc gagattttgc attgggcgga atctgcccga
     1081 accgcgctcg acaactggaa tcaatacggc attttcgccg ccatgcagca attttcccaa
     1141 acacacggca tcgaaacacg cctcttaagc cggaacaacg ggcgcagcct gaccaactat
     1201 ttccaactgc tcgaactgct tgccgccgaa gacgcgcaaa accgcaaccc cgccgcgctg
     1261 cacaaatggc tgcgcgacca aatcagcctg gccggcaaca acggcggcga caaccgcgcc
     1321 atccgtctgg aaagcgacga agatttggtc aaaatcgtta ccatgcacgc ctcgaaaggt
     1381 ttgcagtatc cgctggtgta ctgcccgttt gcgtgggacg cgcaagatac cgggccgtcc
     1441 gactggcaaa tcctccacca aagcgcaaac cgaaccgaac tgttggcaaa ggcgcaactg
     1501 tccgaagacg aacagaaaca atacgccgat gaagaaatgg cggaacgcct gcgcctgctt
     1561 tatgtcgcgc tgacgcgtgc cgaggaacag ctcaacatct acgccgcgta ctccaccaat
     1621 actgccgaca accccctcgc ctacctgatt gaaggctcgc cccaagacag ccgtgaaacc
     1681 gtccgccgtg cctatgcgtg tgaaaaagac ggcatcgcga tgctcaaacg caactggcgg
     1741 cgcgtggcgg acaacgcccc ttccggcaca aatttcgcct tcacagaaga tgcgccgccc
     1801 cctgccgcct atcgcggcaa cgccggtcaa accgccgaat ttgccgcaaa cagcattccc
     1861 gaacgcagat tccgatttgt ccgccacacc agctttaccg ccttaagccg ccatacccaa
     1921 acgcccgacg gcggcgaaga agatgcctgc ccgtccttgg atgccgccga aatctcggta
     1981 gccgcgatgc cgtctgaaac gccgacggct tcagatggca tatcgataca caactttccg
     2041 aaaggcacgc aggcggggct gtgcctgcac gaaattcttg aagatttcaa attcggacaa
     2101 gcagccgccg gacaggaaac cctcatcacc gacaagctga aaaaatacgg atttgaagaa
     2161 atatggctgc ccgccgttgc cgaaatggcg gaagcctgcc gcaaaacgcc gctgacgggg
     2221 gcatacggcc tgtccgacat cccgcccgag tgccgctgtc ccgaaatggg ctttaccctc
     2281 cacaccgaag acttcggcct caaacggcta cgcgattggt ttgcccgcga cgacatcagg
     2341 ctgcccgaag tctgccgtgc cgctgccgaa acgctagact tccacaccgt caacggcttt
     2401 ttaaacggct ttatcgacat ggtctgccaa gaccccgagg gaaatatctg cgtcatcgac
     2461 tacaaatcaa accatctcgg cgcggacgca tccgcctaca cgcggcaggc gatggacgaa
     2521 gccgtcgcgc accaccacta ttaccttcag gcgctgattt acgccgttgc cgccgcacgc
     2581 tacttcaaac tgcgcggaca accgcccgcc gccgtttccg tccgctacct gtttttgcgc
     2641 ggattggatg gcaaaggcgg cggcgtttgg cgttgggaca tagatgccgc cgctttggaa
     2701 cagataaaat aacttcccgt cctttcggac aacaaccgca ggagaaccct atgaatatca
     2761 aacacctgat taccaccgca ctcattgcct cagccgcctt tgccgcgcag gcagccccgc
     2821 aaaaacccct atccgccgcc caaaccgcgc aacatccggt cgtttggatc gatgtccgtt
     2881 ccgaacagga atttagcgaa gggcatttgc acaacgcggt caacatcccc gtcgaccaaa
     2941 tcgtccgccg catacacgaa gccgcgcccg acaaagacac gccggtcaac ctctactgcc
     3001 gcagcggacg gcgtgccgaa gccgcccttc aagaactgaa aaaagcaggt tatacaaatg
     3061 ttgccaatca cggcggttat gaagacctgc tcaaaaaagg gatgaaatga cgcaatgccg
     3121 tctgaaagga caggctttca gacggcatac ggcgttccgg cacgcggcgc aacatcgggc
     3181 gcggcgcgcg ccgccccgtc cgaagcctga taaacaaacc gaaaaggaaa tacgatgaaa
     3241 cacatactcc ccctgattgc cgcatccgca ctctgcattt caaccgcttc ggcacatcct
     3301 gccagcgaac cgcaaaccca aaacgaaacc gctatgacca cgcataccct cacctcaaaa
     3361 tacagttttg acgaaaccgt cagccgcctt gaaaccgcca taaaaagcaa agggatggac
     3421 atttttgccg tcatcgacca tcaggaagcc gcccgccgaa acggcttaac gatgcagccg
     3481 gcaaaagtca tcgtcttcgg cacgcccaaa gccggcacgc cgctgatggt caaagacccc
     3541 gccttcgccc tgcaactgcc cctacgcgtc ctcgttacca aaacggacgg caaagtacgc
     3601 gccgcctata ccgatacgcg cgccctcatc gccggcagcc gcatcggttt tgacgaagtg
     3661 gcaaacactt tggcaaacgc cgaaaaactg atacaaaaaa ccgtaggcga ataagcccgt
     3721 cgccgacata tggcggatgg aaatcaaatg cacacgcccc tcattcccac gaagtcggga
     3781 atccaatcgg ttgggttacc gtgattttca atcattgcag aaacggacga ctccagattc
     3841 ccacccttgc ggggatgacg ggcgtgtgtg tttttgccac atcacaggca actttgccgc
     3901 aaatcttatc tgtcgccggt atgccttctt gtttcaatcc gccacaaacc aaatgccgtc
     3961 tgaaacccga tttcagacgg catttccgtt tattccctgc tgtcgcggca aatatcaatg
     4021 gcttcctgca aactcgaaac gccgtagatt ttcaggttcg gaaactcttt ggcattgcgc
     4081 ggcatattgg ctttgggaac gatggcgcgt ttaaagccga gcttttccgc ttccttgagc
     4141 cgctcttgtc cgcgcgcgac ggggcggact tcaccgctca aaccgatttc tccaaatgca
     4201 accatttttt cgggcagcgg gcggttgcgg aagctggaaa gcatcgcgag gatgaccgcc
     4261 aaatccgccg ccggttcgcc gattttcaca ccgccgacgg cgtttaaaaa cacatcctga
     4321 tcgaaacagg cgatgccgcc gtggcggttt aatacggcaa gcagcatcgc aaggcggttt
     4381 tgttccagtc cgacggtgag gcgtttgggg gtgaagccgt gtgcgtcatc gaccaatgcc
     4441 tgaatttcga ccaaaagcgg gcggctgcct tcctgtgtaa ccaaaacgca cgaaccgggc
     4501 gtgtcgtcgc ggtagctggc aaggaagatg gcggacgggt tggacacgcc tttcaaaccg
     4561 ttttcggtca tagcgaacac gcccagttcg tttgccgcgc cgaagcggtt tttgatggcg
     4621 cgtatcatgc ggtagttgga atgttggtcg ccctcgaaat acagcacggt atcaaccatg
     4681 tgttccagca cgcgcgggcc ggcaatcgcg ccgtctttgg tcacgtgtcc gaccagtatc
     4741 atggcgatgc ccatctgttt cgccatgcgc gtcagttggg cggcacactc gcgcacctgc
     4801 gacacagaac cgggggcgga agtgatttgg tcggaataca tggtttggat ggaatcgata
     4861 accacgactt cgggctgatg ctgtttcaac gctgcttgaa tcgcttccat gcggatttcg
     4921 gcaagcaggt tcacgccttc ggtgggcagt tccaaacgct gcgcgcgcag ggcgacttgt
     4981 tgggcggatt cttcgccgga aacgtatagc actttacggc tttgcgccat tttggcgatg
     5041 gtttgcaaca gcagcgtaga tttgccgatg ccggggtcgc cgccgagcag gatgaccgca
     5101 ccatcgacca aaccgccgcc caatacgcgg tcgagttcgc ccatgccggt cggattgcgc
     5161 ggcacttcgg tggcggtcac ggtggagagg gactggacgg tcgaggtatc cgccgcccaa
     5221 gattggaagc gggcgttttt cggctcgggc gcggcaaggc tttcctgaag cgtgttccac
     5281 tcgccgcaat gcgggcattt gccctgccat ttcggggaag tgccgccgca ttcggtgcat
     5341 tggtaaaggg ttttaagcgt ttttgccatc ggttttccct gcatctgaaa acaaaaatgc
     5401 cgtctgaaat agggacggtt gggattacgc caatacggca acaccgccgc cgtcatgtac
     5461 tatccataca cggcggatat tgccgcattg ccttattttt ccatccctcg gcttcagacg
     5521 gcatatcggg cggtttcctg ccggcttacc cgccgtgata ctgccgagac agctcgtgta
     5581 cggtatcgac taagattttg gcgtgttcgg ggtcggcgtg ttggttgatg ccgtgtccga
     5641 ggttgaagac atggccgctg ccgtgtccgt agtcggctag gatacgtgcg acctcggtgc
     5701 ggatggattc cggcgtaccg aagagggcga acgggtcgaa gttgccttgc agggcgactt
     5761 gcttgccgac gcggcggcgt gcttcgccga tgttacacgt ccagtccaag cccaatgcgt
     5821 ctgcgccgat ttgggccata ctttccagcc acagcccgcc gcctttggca aatacgataa
     5881 caggcacgcg gcggccttcg ctttcgcgtt tgagtccggc gacgatctgg cggatgtatt
     5941 tgaggctgaa ttctttaaac gccgcatcgc tcaacacgcc gccccaagtg tcgaaaatct
     6001 gcaccgcctg cgcgcccgcg tcgatttggg cgttgaggta ggcggtaacg gcttgggcgt
     6061 tggtatcgag gattttgtgc agcaaatcgg ggcgtgagta catcatggtt ttgatggtgc
     6121 ggaattcttt gctgccgccg ccttcgacca tataacaggc gagcgtgaac gggctgccgg
     6181 agaagccgat aagcggtacg cggccgtcca gtgctttacg gatggagctg acggcatcaa
     6241 agacgtattg cagtttttcc atatcgggaa cgtgcagctt ggcgatatcg gcttcgtgtt
     6301 gcagggcgcg tttgaatttc gggccttcgc cttcggcaaa gtacagtccc aagcccattg
     6361 cgtctgggac ggtcaggatg tcggagaaca aaatcgccgc gtccaaatcg aagcgttcca
     6421 aaggttggat ggtaacttcg gtcgccaatc cggtgttttt gcacaaatcg aggaagctgc
     6481 ccgctttcgc gcgtgtggct ttgtattcgg gcagataacg ccccgcctgg cgcatcatcc
     6541 aaatcggcgt gtattcgacg ggctgtttaa gcagggcgcg gaggaaggta tcgtttttta
     6601 aaagagtcat acgggttttc tgtcagtctg tttgtcaagt gaaatggttt tcagacggca
     6661 tttcaacgat gccgtctgaa ggtttcaatg ggtttcggcg gaaggtcggt tttcctcggc
     6721 aacgcttgcc aaaaccaagt tgcgctgcgc ctccgccttc tgcggttctc cggtttcgtc
     6781 aaaaaccttt gccagaacca aacgcgcgga aatactcggc tttaatgcaa tgctcgcttc
     6841 aaggtagcct tttgccttgc cccaaagttt gcggccgtag gcgagccgac cgagatacat
     6901 cagcagaagc gcgttatcgg gctgttcttt cagccaagca tcggcaaaat cgatggcttt
     6961 ctgctgttcg cgttcgccca aaaagcgcac gctttcgaca aaggcttcca aaagctcggg
     7021 gcggcggttg tgcggataat gctgtttgac ccatttgacc gcatcggcat acagtcccaa
     7081 acgttcgtac ttttccgcaa ccgatacgct caattccccg tttttgaggc tgtcgggaat
     7141 ccgcttcagg caggttttca aagcggcggc atcggcagca tccgccagct ggcggcggta
     7201 tgcccaattt tgataccgtt ccatttccga tttgcccaac gcgcccgcct tggaaagttt
     7261 ttcggttttt gccagaacct gcaacgcgtc gcccctgtcg aaagcgtaac gaagttgcag
     7321 acgcacgagg cgcgtaaggt tggcattcat cttcgccgcc gcatgaagat tggcttccgc
     7381 cgcttcgtaa tcgcgccggt tcaacgccga ttccgccaac aaaagataac gggaaagctg
     7441 ctgcttttcc ggcagtttgg cgatttccgc aagataacgg tcgcgcagct cgatgttttc
     7501 catctgcccg gcggcatgtg cgcccaacat caatgccaaa gtccggttgt ctccggcctc
     7561 tttgttgacc aacacgcgtg aggcttctag ttccgccttt tcaaaacgcc cttcaaaata
     7621 cgccaaaccc gccttgttca aggcaagcgc ggccttgcgg cctttacgcg ccgaaccgaa
     7681 acgctgcatc ttttcgggga tattgaatac gccgataatg aatttaaaca ggaaatacca
     7741 caccacgacg gcaatcagcg aacctaacac aaaggcgtgc aggttgattc tgagcatggt
     7801 ctgtccgagt acgatataca cgtcgccggt gtaaatgccc gaagccaatg ccagcccgac
     7861 ggcggcggca aacaggacga caatccagac taccgttttc atgcgcgttc tcctttagcg
     7921 gacggcgaac gccctgcctt ttccggtacg ggctgttttt ttgcaggctg ttccggtttg
     7981 cgttccgaag gcagggacgg tgcttctatg gtcttcacat ccgatgcggc agccggttcg
     8041 gaagctgtgt tttcgtttgc cggttcggaa gccgcctgtt ctgcttcttg attttccgcc
     8101 gccgtcatgc gcgtaccgtc gcgataggcg cggacggcat ttaggctgtt tttcaaaccg
     8161 tcatccgccg tcatgcgcac atccaacgcc ttcaattccg ccagttcttt cagccacgac
     8221 tgcgtggcgg gagacttggc atcgaaatac tgtctgacgg cggcttcggc attgttcaaa
     8281 tcgccctgat agacttcgct gttgcgctgc attaatgcag tccgcgcatc caaaaggcgg
     8341 aggcgcaggt tttcacgcac aaaatatgcc tgttcgggag aaatcagcat ggcatcgttg
     8401 ttttccaaac ggcggatttc gaccagcccc ttcaatgtgc cgagggattt ttcccatacg
     8461 ttctgccacc atgaagcaga agcggcttcg ttcttcacct gtacgcccgg cttcaatacg
     8521 ccgtccaaaa tcagcggcag tccggatacg gcggtttcca gcctgtcgag gcgcaatgcc
     8581 gtgccggaaa tatcgacata gggacggttt ttcagttccg ccaagtcgct gctgaccgcc
     8641 tgtttgatcg gcagaagctc tgcctgattg aaacgggaca ggcggctgtc gatatgctcc
     8701 aatacgccga ctgccgtttg gatattgccg gtcaacacca gctgttgcgc cgccagattg
     8761 agtatggtct cggtttcgtc caccagccaa tcggcgcgtc ctttggtcaa ctcgcgatag
     8821 gatttttgca tttccaagat ttgttcgccg tttgctttga cgtttccgtc caaacggtcg
     8881 agttctgatt gtatggcggc ttgccggttg aggttgtctt tgagcagggc ggcgttttcc
     8941 gactcgccca aggcggcttt gtcgattttt tggttgaatg ccagctcttg gtttttcaag
     9001 acattctgtc cttggacaaa caaaaaacca cttgcgccca aacccaatgc cgccaatacc
     9061 aaggcgcagg ttgccaaagc gttgttgccg gactgtttga cgataaaagg agcggcagga
     9121 atgtgtactt ctgtttcgtt ttctttgcgt ggggaagagg tttcagacgg catttctttt
     9181 gatgcctgta tctcgcgtac gggttcggat gatttgtttt caggttcgcc caccgttttg
     9241 ctccttatta atttgaggtt ccgggaaaga ccatgccgtc tgaaacggaa atggaagaat
     9301 gggaaagcgc ggcttccagc gtagggacgg tttcgaccga acacacgcct tcgcgcttca
     9361 atgcctccgc aatgcgcgga tgatgggtaa agtatagcaa ggatttgaag aatcgggaaa
     9421 attgcggcgg aagctgtccg aacagcaagc gcacgagttc tgtggacgta atataggcgg
     9481 cggcaatatt ttcggtttgg aaattttgaa agttcaaagg tttatgccgt ctgaaataga
     9541 cttctgccac ctccgtccga aaacctttct cctgcaaggc attcatcaga aaatcccgcc
     9601 cgccgtgtcc gcgcacaaac aatacgcgcg caccttcggg cagactgttc caaaccgtca
     9661 ggcgcaaaac cgcctcgctg tcgttgccgt cttcaggcgc gatgaccgtt ctgcccaaac
     9721 agcgttccaa tgcacggcgg ctgccctgcc ctacggaaat gtgcatcttt atgccgtctg
     9781 aaaggttaag gtacgggacg gcggtttcaa cggcggtcgg actgacccaa aacacggcat
     9841 ccgcacgggc atattgttcg gacaaaagtt ccagtccggc agcatctgct tcgatttcaa
     9901 tcgggctcaa cacttccgcc tgccatccgg cacgacggca ggtttccaca tcgtccttcg
     9961 ccctgcccga cggtcggaca atcagcatga tgggtttcac tgtttccatc gccttatccg
    10021 ccccgattac cgggaaaaat cagcatcgtt gaaattcgcc tgcgcggttt gcggcgtaca
    10081 gccaacccgt gcgacttcct tgcccctacg ctccacaaca acaccgctgc tttccgtatg
    10141 agtcacggaa tcgaagcccg tccatacggt gtacataaat tcgccgtttt ggaatttcat
    10201 cgttgcccgg cgtccgctac ccatgccttg ccacctgtcg gaacgcccca acaggtcagc
    10261 tttgctgttg cgtatggcaa tttctttttt tgccgcactg ccgaacgaat attcgtaaag
    10321 attgcggttg attttttgaa cttctatata tttgttgttg tccgttttac aggaaaacac
    10381 cgtatccgcc cacaccgttt gggcggcagc cgacagaagc aatacagcag aaaggaacgt
    10441 ttttttcatc gtatttcctt aatggttaaa accccgccac ttggacatcc gtccttcgtg
    10501 gcggcagcat cagattttat ttgagagggg tgtaacccct tccaaattag ggcaacacat
    10561 agggcaacgc tttatgtgtc gtcctgtgtg ttgaaacatc attgcccgta tcctgaaata
    10621 gaaaagaaaa tgcccgtttt acggtcttct ttccgacaaa atgccgtctg aaacggattt
    10681 cagacggcat ggtcgaaaat tattgcgcgg caagcggctt gagcagcaag gtttcgccgc
    10741 tttgcacaac tttttcaagc aggtcgatat tgggatgctt gccgggatgg gcgcgcgcgt
    10801 cggcaacgtg ttcggcaaac catatcaaac cctgttctgc cgcttctttg tccaattttc
    10861 cgtcaaaacg ttctgccaaa gcattgtaca gcttgagcga accgagctta ccctgaacgg
    10921 cgggaatgtg gtgaaccgtt ttgccgtcgg catcctgaac gtccaaaccg cccagatggt
    10981 cgattgaagg aaatgtggcg agatagtctt ggaaattcat atcaaatccg aaaatcagtc
    11041 agttagcgcg tgccgaaaat cttgtcgccc gcatcgccca agccggggat gatgtagccg
    11101 ttctcgttca agtggctgtc gagcgcggcg gtgtaaatcg taacgtcggg atgcgcgtcg
    11161 ttgaccgcct tcacaccctc gggcgcggca accagcacca gtgccttgat gtttttgcag
    11221 cccttggctt tcaacaggtc gatagttgcc accatcgaac cgcccgtcgc cagcatcggg
    11281 tcgataatca gggcaggacg ttcgtccatg ctgtccacga atttctcgaa gtaggaaacg
    11341 ggtttcagcg tttcctcgtc gcgctgcagt ccgactacgc tgattttggc agtcggaatc
    11401 aggtcgagta cgccgtcaag catacccaaa cctgcacgca ggatgggaac gacggtcaat
    11461 gttttgccct tgatgcgatc gccttcaatc tgaccgcacc atccgtcgat aaggtatttt
    11521 tcgatttcaa aatcacggct tgcctcgtat gccatcaggc gcgccagctc gatggcaagc
    11581 gtccggaatt tgtaggtgct gcaatccgcc tccctcatca gggttaattt gtggcggacg
    11641 agcggatggt tgataacatt aacgttcatc gcggtgttcc agtgtgattt tgagacgcgc
    11701 cattatactt ttttttgatt tgccggaaaa gaaaatgccg tctgaaacac gttcagacgg
    11761 cattgccgaa tccatcaggc tttcagcagc tcttgcagct cgcctgcctc atacatttcc
    11821 atcaggatgt ccgaaccgcc gacaaactcg ccgttcacat aaagttgggg gatggtcggc
    11881 cagtcgctgt attccttaat gccttggcgt acttcgggat tttccaatac gttgacggta
    11941 acgtaatcgg tgcagcccgc cgcgttcagg atttgcacgg cgcgggaaga gaaaccgcat
    12001 tgcggaaact gcttcgtacc tttcataaac aatacgacgc ggtgtgtcgt tactacttct
    12061 ttaatttggt cgtggatgga agccatcgct tattcctttt gataaagttg acaaaatttg
    12121 tcaggtagga cgcattatac gcaaaagcgg aaacttccca caatatttgc cgtatatcgc
    12181 gcagtatcaa tgttgtgaac ggaaatattc ccactcctcg acaatcctgc tgtccggcaa
    12241 atggcacagg gcatatccgc cgccgctttc gtctttttta cttcccgtct gccgcctttg
    12301 gcaatgcaga atttggaagc ggaatttgcc atgcctgtcc gaaacgtgtt ggcaggcagc
    12361 cggcagcagc gcggacatac atacggatga attgccttca tggttttcct tatcgtcggc
    12421 acagggtcag acggtccgca tatcgggtac ttttttaaac agccggtaaa agttttccgt
    12481 cgtgtatgcc gcaacctgtt ccaatgtttg gttccgcaat ttggcgatat gttcggcggt
    12541 atggcgcaca aaagccggtt cgttctgcct gcctcgtttg ggaacggggg cgaggaacgg
    12601 cgcatcggtt tccaccaaaa tgcggtcgtc cggcacatat tttgccgcct cctgaaccaa
    12661 gggtgcgttt ttaaaggtaa cgattcccga gaaagaaata taaagcccca aatccattgc
    12721 tgcacgggca aaaccgatgt cttcggaaaa acagtggata acgcccgaat taacccggca
    12781 ttctttcagg atagacaagg tgtccgccgc cgcatcacgc gtatggacga taacgggcag
    12841 tccggtttga ttggctgctt cgatgtggtc tgcaaagcgt ttgtgttgcc aggacaaatc
    12901 gcctttgcac cagtaataat ccaaacccgt ctcgccaatg ccgaccactt tcggcgaggc
    12961 ggctgcggca accatttccg caatagaaaa ttcttcggct tccttgctgt cgggatgtac
    13021 gcctattgtg cagtaaatat gttcataaac ttcggcgata gcggatactt cggcaaaact
    13081 ttccctgctg acgctgatgg cgagcgcctg ccccacgccg tttgcttcca tgttggacaa
    13141 aacttcgggc aggcgttctt tcaaaccttc aaaattgagg tggcagtgcg aatcgatgat
    13201 atgcatggcg ttacatggta aacgtaggtc tgctgccgcc gattgtgttg cccagttcga
    13261 cttcgatctg gtctttgact ttcaggcagt tttcgtgttt tgtgaatgcc agccccaccc
    13321 ctttgggttt ggaggaagta cgcgcaggat tgatccaggc gactttggtc ggcaggaaca
    13381 gtttggggaa gttgaggatt tctacggcaa gcagaatatc gtccccgatg gaaaatacgt
    13441 cgttggtctg cacaaacaga ccgccgtgtt ccaaaaacgg catgtaggag ctgtacagca
    13501 gattcatgtc tttcagctgc aacgacatca tttttgccgg aatattttgt ccgtctgaca
    13561 taattcaccc gttctctttc ttcaattcca aatagttgat gagcagatgc tcgatctgca
    13621 ttttgacatt taaagtatga aacccgtagg gggcaagctg tttgagcata tcctccgccg
    13681 caaatacatt gcgcaggcgg aaaccggatg ccgtctgaag cagcctgtct tcataagcgg
    13741 gataatagac gggtttcatg tgttgcaggc acaatcccaa atcgaccagc catttctgca
    13801 tccacccgac aaatacgtcg agcggaagtt tctccttatc gaaaagcgcg gcgtaatcca
    13861 aaatcttcaa caaccttggt tctgccaaaa tatccaacag tttaatccgc aacgcacgga
    13921 caccgtccgc ctcatcaaac agcggcgctc cggaatggaa agccagacgt tcctcaggtt
    13981 ccgccacacc cctttcacgc agatatgcca atgcctcttc atgggaaggc gcgggcaaaa
    14041 ccatcttccg gcagcggctt ttaatggtcg gtaaaacctt gtccgccgcg tggctgacca
    14101 gcaaaaagac cacttgtggc ggcggctctt ccaacacttt caacaaactg ttggcggctt
    14161 ggacattcat actttccgca ggatgaatca gaatcacgcg caaaccgccc cgtaccgaag
    14221 tcaggtacac attatcgatg atttccctga cggcatcgat tttgatctgc aacagtttgc
    14281 gtccgttttc gggttcgtcc gacaaggggg tgatttcgta aaaatcggga tggcttccct
    14341 gtccaaacag atggcaggac atacattcgc cacagggttt gcagcccggt gcaggggttt
    14401 cgcacaacag tgccttcgcc gcaaagcggg caaatgtagt tttccccgta tcttttttgc
    14461 cggcaaacag ccatgcattg ggacgacgct cccaatgttc cgcaatctgc cgccattgct
    14521 cattatgcca cggataaatc atttcaaacc atccgtccgg aaatccatac cgccatcaga
    14581 gcagttccaa atcggggctg gaactttgcg cgcggcggct gcgcaactgt accgccaaac
    14641 gcaaatcgtg tgcggaatcg gcatttttca atgcttcttg cagggaaata tcgccttttt
    14701 catacaattg gtaaaggtgt tgatcgaagg tctgcatacc cagggtagtg gattttttca
    14761 tcacttcttt gatttcatgg atgttgccgt tgtgaatcaa ctccgaaatc aggggcgaat
    14821 tgagcagcac ctcgactgcc gccaccctgc ccttgccgcc gtctcgcgga acgaggcgtt
    14881 gcgaaataaa cgcctgaagg ttgagcgaca aatccgtcag caattgttcg cgccgctcct
    14941 cggggaaaaa gttgatgatg cggtcgagtg cctgattggt gctgttggcg tgcagcgtcg
    15001 ccatacacaa atgccccgtt tcggcaaagg caatggcgta gtccattgtt tcgcggtcgc
    15061 ggatttcgcc gataaggatg acatccggcg cctgacgcag cgtgtttttc aacgccgcca
    15121 tccagttttc cgtatccacg ccgacctcgc gctgggtgat gatgcagttt ttgtgttcgt
    15181 ggacaaactc gatcgggtct tcgatggtga tgatgtgtcc gaacgaattt tcattgcggt
    15241 aatcgataag cgaggcaagc gaagtcgatt tgcccgagcc ggtgccgccg acaaaaataa
    15301 ccagcccgcg ttttttcagc gcgacatcct tcaagaccgg cggcaggttc aggctttcaa
    15361 acttgggaat cttgctggta atcgcacgga ataccaacgc cgtcgcgccg cgctgtatca
    15421 tcgcattgac gcggaagcgg ctggtgtccg gcaggctgat ggcgaagttg cactcgttgg
    15481 tcgatgaaaa ttcttccgcc tgcttcgcac tcataatcga aaaggcgatt tccatacatt
    15541 tttccgccgt cagcggttcg tccgtgatgc gggtgatttt gccgtccagc ttcattgcgg
    15601 gcgggaaatg ggtcgtcaca aacaggtcgg aacctttgtt tttgttcata tggcgcagcc
    15661 atgcgaacag ttcttccttt gcggtcatat tttcatcggt aaacatcctc gtcccctaat
    15721 cttcaaacaa tctgtacctt cccattctac caaaaacccg ccgtgccgtc tgaaaccgtt
    15781 tcagacggca cggacaaacc gcccccactc aatcctgccg tgttagcgtg tataatccgg
    15841 cattctatcc gaaattcaca acaggtcagc aatatgtctt tgaaaacagt agccgttatc
    15901 ggcgcaatgg aacaagaaat caagcttttg cgcgagatga tggaaaatat caaagccgtc
    15961 tctttcggca gattttccgc ctatgaaggc gaattggtgg gaaaacgcat ggtgcttgca
    16021 ttgagcggca tcggcaaggt caacgcggcg gttgcaacgg cttggcttat ccatcaattc
    16081 gcaccggact gcgtcatcaa caccggcagc gcgggcggtt tgggcaaggg tttgaaagtc
    16141 ggcgacgtag tcatcggtac ggaaaccgca caccacgatg tcgacgtaac cgcattcggc
    16201 tatgtttggg gacaagtgcc gcaactgccg gcaaggtttg cttcagacgg cattttgatt
    16261 gaggcggcaa aacgggcggc gcggacgttt gaaggcgcgg cggtagaaca aggcctgatt
    16321 gtcagcggcg accgcttcgt ccacagcagc gaaggcgtgg cggaaatccg caagcacttc
    16381 cccgaagtta aggcggtaga aatggaagcg gcggcaatcg cccaaacctg tcatcagttg
    16441 gaaacgcctt tcgtcatcat ccgcgcggtt tccgattcgg cagacgaaaa ggcagacatc
    16501 agctttgacg agttcttgaa aacggcggcg gcaaattccg caaaaatggt ggcagaaatc
    16561 gtcaaatctt tataaaacct ttatcaaatc atgccaaaac agcggttttc cgttagaata
    16621 tcgggctatt tccacgttgc gcccgatatt tggacgtacc gctgtttgtt cagacggcat
    16681 ttctaaaacc taccgttcag cattaaaaac aactcaatga aaaatatccg aaatttctcc
    16741 atcattgccc acatcgacca cggcaaatcg acgcttgccg accgcttcat ccaatattgc
    16801 ggcggtttgg atttgcgcga aatgagtacg caggtactcg attccatgga catcgaaaaa
    16861 gagcgcggca ttaccattaa agcgcaaacc gccgcgctca actataaagc acgcgacggg
    16921 caggtgtatc aactcaacct gattgacacg ccgggacacg tcgacttctc ttacgaagtc
    16981 tctcgttcgc tctctgcctg cgaaggcgcg cttttggtcg ttgacgcgtc gcagggcgtg
    17041 gaagcgcaaa ctgtggcgaa ctgctatacc gcgattgatt tgggcgtgga ggttgtgcct
    17101 gttttaaaca aaatcgacct gcccgccgcc gaccccgaac gcgtggaaca agaaatcgaa
    17161 gacattatcg gcatcgatgc cgtcggtgcg gtgcaatgtt ccgccaaaag cggcatcggc
    17221 gtggaagacg ttttagaaga aatcgttgcc aaaatccctg cgccgaccgg cgacgaaaac
    17281 gcgccgctgc aagccgtgat cgtcgattcg tggtttgaca actatgtcgg cgtggtcatg
    17341 ctgattcgag tgaaaaacgg caccatcaaa ctgaaagaca aagtgcgctt tatgagcacc
    17401 aaggcggaaa cgcaggtcga gcagctgggc gtattcacac caaaatcagt tcaaaaacaa
    17461 gaactcaaag ccggcgaagt gggctttttg attactggcg taaaagaatt gggtcaggcg
    17521 aaagttggcg atacggttac actggttgcc aaccctgcca ctgagccgct gcccggcttc
    17581 caagaagtac aaagccaagt attcgccgga ctttatcccg tggaaagcca cgactacgaa
    17641 gccttgcgcg acgctttgga aaaattgcag cttaacgacg cttcgttgaa attcgagcct
    17701 gaagtttccc aggcattggg cttcggcttc cgttgcggct tcttgggtct gctgcacttg
    17761 gaaatcgtgc aggaacgttt ggagcgcgag ttcgacatgg acttgattac caccgcgccg
    17821 acggtggttt acgaagtcgt gttgaaaaac ggtgagaaaa tcgaagttga aaacccgtcc
    17881 aaactgcccg acatcggcag catcgaaacc atactcgagc cgattattac cgcgaccatc
    17941 ctcgtgccgc aggaatacgt cggcaacgtc atgactttgt gtaaccaaaa gcgcggcgtt
    18001 caggtcaata tgcaatatat gggccgccaa gtcatgctga cttacgattt gcccatgaac
    18061 gaagtcgtca tggacttctt cgacaaactc aaatccactt cgcgcggtta tgcttcgttg
    18121 gactaccatt tcaaagagtt ccaaccgtct gatttgatta agctggatat catggtcaac
    18181 ggcgaaaaag tcgatgcctt aagcctgatt gtgcaccgtc aaagcgcggt tcaccgaggc
    18241 cgcgagctgg catcgaaaat gcgcgagctg attccgcgcc aaatgttcga cattgccgtc
    18301 caagccgcca tcggcagcca gattatcgcc cgcgaaaacg tcaaagcact gcgtaaaaac
    18361 gtcttggcga aatgttacgg cggcgatatt acgcgtaaga aaaaactgct tgaaaaacaa
    18421 aaggcaggta aacgccgtat gaaacaagtg ggcaatgtgg aaatcccgca aagcgcgttc
    18481 ttggcgattt tgcaggtgag cgacaaataa tatttcggca catagggcgg cgcgtcggga
    18541 acacctctca aaccgccctg atttcagggg cacgcgcccg caaacaaatt ttgcaatttg
    18601 ccgccttaaa acggttgcac ggccgggcgg gtttttaacc gttgaggaac aacaatgaac
    18661 acaatgctaa tgtcgggcgc ggctgccgcg ctgcttgccg gcatcatcct ttatttcaaa
    18721 agcgacaaga agcggcagga aaacggtgaa tggagttccg gcctcgaata cgcctatatc
    18781 ctgacggcgg tcggcgtgtt tgccgctttg tccctgttta tgagctttgc cgccgttttc
    18841 ctgattttcg ttgtattgtg cggtacggct tgggcggtat acaaataccg cctgaagact
    18901 catcccgaaa tctcggaaag cagccacttc ggcgattatt tcggcagttt cttccctacc
    18961 gttttggtat tgttcctcat ccggtcgttt attgccgaac cgttccaaat cccgtccagc
    19021 tcgatgcgcc cgggcctgat taagggcgat ttcattttgg tcggcaaatt ttcctacggt
    19081 ctgcgcgtac ccgttttaaa caatatattt attcctacag gcaaaatcga acgaggcgat
    19141 gtcgttgttt ttaattatcc tctgcagccg gagatgacct acatcaagcg tattgtcggc
    19201 attccgggcg atgtggtcga atatcgggat aagattttga cggtaaatgg caaacccact
    19261 tccgatattc ctaacggcac ataccgttat cccgacgaca ccgacccttc cgaaatccac
    19321 aacacggata tgttccgcag cgatctggac ggcaaatcct tcaatattct gaaaaaagaa
    19381 ggacagcctg ccgtttccct gcccgtattg ggcaaatata cctccgatat tatgtctgaa
    19441 aacggatatt ccatagagca aagcggtttg gaacactgcc aatatgccga cgacggcagc
    19501 ggtttcgtgt gcaaagttcc cgaaggacgc tatttcgcta tgggcgacaa ccgcgacaac
    19561 agtgccgatt cgcgctactg gggatttgtg gatgacaagc tggttgtcgg caaggcaatg
    19621 ttcattttga tgaacttcgg cgatttcggc aggtccggta cggcaatccg ttaggccgga
    19681 aaattacgcc ggaacatcaa aaatgccatc cgaaccctgt ttcagacggc atttttatca
    19741 tcaaacgcct tgtttccctg ccggcagcct cggggataat atagtggatt aaatttaatc
    19801 cactatattt ccgtttgtgt ggaaacccat cccattgaat aaagggcatt tcgtccgact
    19861 gacggtgtgg gcggcatatg gaaatacgga cattatttta gttgcattgc cattccagcg
    19921 gaggcggctt ttttgattct gcccgccccg atgccgtctg aaagggcttc agacggcatt
    19981 ttccgctccg cgccgttgct ggcgtgtttg gtcttgccgg ttttgactgc ctgccagttc
    20041 aatcgtacgc aggtttgcca cggcagaaaa tctgatgccg gacggagtca aggctcggac
    20101 tgctgtttca gcgaaccgag gtaaattgcc gaaagcgtca gcgccacccc tgcccactgc
    20161 acaccttcaa tcggtttgcc caacccgaaa taatcgatga acagggcggc aaccggttcg
    20221 gacaaaagca gcagccccgt cagcgacagc gaaagcagcg gaatcgcata ggcaaccatc
    20281 gcccacgcga agcactgcat caccacgccg tacacaagca ccaaacccgc atctttccaa
    20341 gtcgtcggat aaagcacgcc gccatccatc agcaatgccg gaacaaccag cgataccgcg
    20401 ccgcccaaac tcaaaatcat cattgaaggg aaaagcgcca ccggctcgat ttcatgggtt
    20461 ttgcggacga acaccatcga cagggcgagc atcagtcccg ataccaaacc gctggcgaat
    20521 ccccaaaccg cattaccgtt gtagccgaat tccgcaccgg caatcatcgc cacgccggca
    20581 actgccgata ttaagcctgc ctttttcagc ccgctcaaac gttcgttaaa aaagaaaaca
    20641 ccgattgccg acaagaaaaa gatttgcagg ctgttgagca gggtggaaat acccggcccg
    20701 accgcgtgta tgctttcgtg ccacaacgcc aaatcgaaag cgaggaacac gcccgccgtc
    20761 agggcatatc ggacggtttt cctgtttttt gggaattttt gcccgaaaaa ccgtgccaaa
    20821 aaccaaaata cgaacaccga aatcagcaac cgccaaaatg cgattgcata cgggccgacg
    20881 gggacggacc tgacaatcag actgcccaga ccgaacacca cgcaacccag caccagcaat
    20941 ggtgcggcat attttttgcc gttaccgacc acaatatccc ctcccttccc catacggctg
    21001 ccgcgccgac ggcaagaatg ccgccattcc gaaaatatgc cgtctgaagc ccaaagcccc
    21061 ggctgcaccc ggacaaacgg ctgattttac gggcaggccg actattttcc gttggggcaa
    21121 aaaaccgtcc tgccagataa aacccgaata tagtggatta acaaaaacca gtacagcgtt
    21181 gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt
    21241 tccgtactat ttgtactgtc tgcggcttcg ttgccttgtc ctgatttttg ttaatccact
    21301 ataaaaaata tttccctcaa gcaatgcggc ggcaccaatg cctgattaaa acacaaccgg
    21361 cgttttcggc atatatgcaa aaacgcattt tccggcacgg cgttacaccg ataccgaaaa
    21421 atgcgtctga atgtttcgac aatccgatta aagcattatg ccaaatctgc aaaaagtggc
    21481 gtagagagat agcgttcgcc ataagaaggc agcagcacga ctatcagctt gccttcgttt
    21541 tcaggctgtt tggcaagctg caacgcactc caaaccgccg caccggaaga aatacccacc
    21601 aaaatgcctt ctttttccgc tattgcgcgg gcggtttcaa aagccgcttc gttcgacact
    21661 ttggtaatgc tgtcgtaaat tttggtattc aaaacggtcg gaataaagcc tgcgccgatg
    21721 ccttgaatcg ggtgcgggcc tttttcgccg ccgctcaata cgggtgaagc ttcaggctcg
    21781 acggcaacca ctttaacttc gggtttgtat tttttcaaca cttcgcccac gccggtaatc
    21841 gtaccgcccg tgccgacgcc ggcaacgaag acatcgactt tgccgtccgt atcccgccaa
    21901 atttcctcgg cggttgtttt gcggtggact tcggggtttg cctcattgtc gaactggcgc
    21961 ggcataaaat aagtgtcggg atgcgcgtcc accaaggatt tcgctttggc aatcgcgccc
    22021 gccatacctt cggcggcagg ggtcagaatc agctccgcac caaacgcgcg caacagcatt
    22081 ttgcgctcct tactcatgct ttccggcatg gtaatcgcca gcttgtagcc gcgtgcggca
    22141 cataccattg ccaaaccgac acccgtattg ccgctggttg cttcgacaat gacggtgttt
    22201 ttgttgattt tgcccgcttt ttcggcaccc tcgatcattg cttcggcaat gcggtctttg
    22261 acgctgctgc ctggattgaa aaattccagt ttcacggcaa cctccgcctt caaaccttcg
    22321 gtcagacggt tcagtttgac caaaggcgtg ttgccgatta gttcggtgat gctgtttgca
    22381 attttcatct tctgctcctg aataataagg tttcgacagt aaggcgcaat ccccataggc
    22441 atcctgtccg attgcagggg atttgcgccg aaacataaac aaatttcgga tatggcttta
    22501 tcctaaacct atgccgccta aaaaaccaat atccaaaatt tctttcttta taactttttg
    22561 ttccgatacg gccgcaccgt attatcttcc aatttctgaa agcagcagca ccagtaccga
    22621 gacctgcatc aacgctgccg tcaacccgcc gagtcgcagc tgcgagccgc cgaacgttga
    22681 cggcggctgc cggtctatac cgcagcggtg gacataggca accgccatca gcacaaactg
    22741 caacacgaac caataaaccg gcttcagcgc ataaagtttg agcagtgccg caaacacaaa
    22801 cacttgaacc gttcccgaaa agcaataatg caccaacgca catattccga caaaagcaag
    22861 cgtcatcgat acattactga cggcaaaaag cccgagcagc ggatgttcgg ggtctgcctg
    22921 ccatccgttt ccatccgttt tccggttcca atgcccgatg aaaaaaaata tgctgcccaa
    22981 agtcaggtaa aaatggggga tgaacaaggg cgcggcgcgg gtcattcccc atatgccggg
    23041 catcagcttt gcccccaaaa ccgatatgcc cagccacagc ataatactcg cccaaaacca
    23101 ttgcaacatt cctgcgcgga atatcagaaa cagaaaaacc gccgcataca gtaaggcaga
    23161 caaaaaatcc aaatgcagca aaatcatgaa tacgccaact cgccttcaaa caccgtttcc
    23221 gcagggccgg tcatcatcac atcgccgccg caggcccatt cgatatataa agttccgccc
    23281 ggcaaaatca cctctaccgt tttcccttca tccaacaacc ccagacggat acccgccacc
    23341 acagccgcac acgcgcccgt accgcacgct tgggtttcgc ccacgccgcg ctcgaacacg
    23401 cgcaaacgaa tcgcggttcg gctgacaatc tgcataaagc cgacgttgac gcgttcggga
    23461 aactgcctgt gcggttcgat aagcgaaccg gtttcgcgca cctgcgcgca ttctacgtca
    23521 tcgaccacaa tcaccgcatg ggggttgccc atattgacgc agctgacagg ctgaatgccg
    23581 gattcgagat gcaccccgta aatacaggca tcatccccct cgcccgattc ggggacaaac
    23641 ggtatttcag acggcataaa cttcggtttg cccatattga ccgtaaccat gccgttatcg
    23701 gacaatttcg gaaaaataat gcccttggcc gtttcaacac aaatttcttt cttatcggtc
    23761 aaacccttgt ctgcaacaaa acgggcaaaa caacgcgctc cgttgccgca ttgcccgacc
    23821 tcgctgccgt cggcattgaa aatacggtaa cggaaatcca caccttcggt ttccgaacgc
    23881 ccgaccacca aaagctggtc gaacccgatg ccgcggaaac ggtcagccca tgctgcaatc
    23941 ggtgcatgct caggaataaa atcctgactg accgcgtcaa tcaccataaa atcgttgccc
    24001 aaaccgtgca ttttagtaaa tttcaaagtg ttcatattat tctgaaatcc cgtattatct
    24061 gttgcttccg aattccgaaa tcccccgcac cgaagggaaa tgcttccatc tcccgccgcc
    24121 tgaggattaa aatcagccat tatactgttt gttacccctt acaagaaaca ccatgttccg
    24181 catattactg ttgcttttcc cttgtgcaat ccttgtcgcc tgcacccgaa acataccggc
    24241 aaacccgacc gtttcaaatt gcgcgcatct tcaaaaccgc accatcctgt attttgacgc
    24301 acacggcaaa cccgccgacg gcgcagtaaa gggcggattt tacaaaatta tcgaaataca
    24361 gggtgcaaac tgcatcctta ttcaagactt taacagcacg ggcaatgccc tcggcggatc
    24421 ctatgtgccg gactgccgca aatcatccct tttcccgaaa aatatttgac ttcaaactgc
    24481 ctatgaacca cgacatcact ttcctcaccc tgttcctgct cggcttcttc ggcggaacgc
    24541 actgcatcgg catgtgcggc ggattaagca gcgcgtttgc gctccaactc cccccgcata
    24601 tcaaccgctt ttggctgatc ctgctgctta acacaggacg ggtaagcagc tatacggcaa
    24661 tcggcctgat actcggatta atcggacagg tcggcgtttc actcgaccaa acccgcgtcc
    24721 tgcagaatat tttatacacg gccgccaacc tcctgctgct ctttttaggc ttatacttga
    24781 gcggtatttc ttccttggcg gcaaaaatcg agaaaatcgg caaaccgata tggcggaacc
    24841 tgaacccgat actcaaccgg ctgttaccca taaaatccat acccgcctgc cttgcggtcg
    24901 gaatattatg gggctggctg ccgtgcggac tggtttacag cgcgtcgctt tacgcgctgg
    24961 gaagcggtag tgcggcaacg ggcgggttat atatgcttgc ctttgcactg ggtacgctgc
    25021 ccaatctttt ggcaatcggc attttttccc tacaactgaa aaaaatcatg caaaaccgat
    25081 atatccgcct gtgtacggga ttatccgtat cattatgggc attatggaaa cttgccgtcc
    25141 tgtggctgta agccccattc attcccgata cccgtaaaaa ataccgtctg aaacctgttc
    25201 agacggcact ttcaccctaa gcgttactcg gcggcatttt cctcacgggc aggcgcgtcc
    25261 agcagggctt tgatggacag gcgcacacgg cctctgtcat ccacttccaa tgctttcacg
    25321 ttcaccacct gaccgacttg caggtagtca ccgacattgc gtacgcgctc gtgggcgatt
    25381 tggctgatgt gtaccaaacc gtctttgccc ggcatcacgc tgacaatcgc gccgacgttg
    25441 ttgtcgagga ttttcaccac agtgccttca tacactttgc ccacttccac ttcggcagta
    25501 atctgctcga tacgtttttt cgccgcatcg ccggcttctt gagtggtcgc agcaatggta
    25561 atcgtaccgt cttcggcaat attgatttcc gtaccggttt cagcggtaat cgaacggatg
    25621 gtttcgccgc ccttaccgat aacttcgcgg attttgtctt ggttgatttt catcgtaaac
    25681 aaacgcggcg cgtgtgcgga caactcttgc gggcccgcaa cggcggcttt catctgatcc
    25741 aagatgtgca gacgcgcttc tttggcctgt gccaaagcga tttgcataat ttctttggta
    25801 atgccttgga ttttgatgtc catttgcagc gcggtaacgc cttcggtcgt accggccact
    25861 ttaaagtcca tatcgcccaa gtggtcttcg tcgcccaaaa tgtcggtcag gacggcaaat
    25921 ttgttgcctt ccagaatcag acccatcgcg ataccggcaa cgtgtgcttt caaaggcacg
    25981 ccggcagaca gcaggctcag gcagccgccg cagacggaag ccatagagga agagccgttg
    26041 gattcggtaa tttcggagac cacgcgcatg gtgtagctga aatcttcagg tttcggcaat
    26101 acggccaaca atgcacgttt agccaaacgg ccgtgaccga tttcacggcg tttcggcgca
    26161 cctacgcgac ccacttctcc ggtagagtac ggcggaaagt tgtagtgcag catgaagcgg
    26221 tcggtgtatt cgccggacag cgcgtcaatg atttgctcgt cgcgcgaagt acccaaagtt
    26281 gcaaccgcca aagcttgggt ttcgccacgg gtaaacaatg cggaaccgtg tgtacgcggc
    26341 aatacgccgg tttggatgtt caaaggacgg acggtgcggg tgtcgcggcc gtcgatgcgc
    26401 ggttgaccgt ccaaaatttg gctgcggaca acatcggctt ccaagtgttt gaaaatacct
    26461 ttgatttcgt tggcggccaa agtatcggtt tcttcggtaa tcaaggcttc ttttaccgca
    26521 ttccaagcct cgtccaattt ggcggaacgc gcttgttttt gacggatttt gaacgcttct
    26581 ttaatggttt cgccggcaat cccgcggact ttggcaacta attcttcatt ggtttcaggt
    26641 gctttccaat cccaaacttc cggattgact tcgtcggcaa attcattgat ggcgttaatc
    26701 gccgcctgca tttgatcgtg gccgtaaacc accgcgccca acatcacgtc ttcgggcagg
    26761 attttggctt cggattccac catcaacacg gctttggacg taccggcgac caccaagtcc
    26821 aattgcgatt tagccaattc ggctttagtc gggttcaaaa cgtacacgcc gtttatataa
    26881 cccacgcgtg ccgcaccaat cggaccggca aacggtacgc cgctcaacac cagcgcggca
    26941 gacgcgccca acattgcagg aatgtcggaa tcgatttcgg gatcgacgga cacgaccatc
    27001 gctacgattt ggatgtcgtg gtagaaacct tcggggaaca gcggacgaat cgggcggtcg
    27061 atcagacggc tggtcaggat ttctttttcg ctttgtttgc cttcgcgttt gaagaaaccg
    27121 ccggggattt tacctgcggc gtaagtgcgt tccaaataat cgacggtcag ggggaagaag
    27181 tcttgacctt ctttcacttc tttgttggtg gtaacggcaa ccaaaacaac ggtgtcgccc
    27241 atagagactt taacggcagc ggcggcttgg cgggcaattt cgccggtttc caaagtaacg
    27301 gtgtggttgc cgtattggaa agttttaacg tgtttgtcga acattattgt tcctttcaaa
    27361 ataccgcact gctaaaacac taataatgca cactaaaatc cgaatgtgca tagttagggt
    27421 ttcagaccgt gcggcaggtt gtaaacaagc tttcagacga catttcggac gctgaaagca
    27481 attgcggatt ataaaggcaa ccatccttaa aatccagtat tgatacaata aaaaggccgt
    27541 ctgaaacata tctctttcag acggccttca aacttaaaaa tcaatcctga gtcagcaaag
    27601 tcaatgcttc ccgatacttc tcgacagttt tctgaatcac atcggcaggc actttcggcg
    27661 caggggcttt tttgttccaa ccgctttgtt ccagccagtc gcggacgaat tgtttgtcaa
    27721 aagacggcgg attggtgccg acttcatatt gatccgccgg ccagaagcgg ctcgaatcgg
    27781 gagttaatac ctcatccatc agcgtcagcg taccgtttgt atccaaaccg aattcaaatt
    27841 tggtatcgca aataataata ccgcgcgatt tggcatattc cgccgcttcg gtgtaaagcc
    27901 gaaccgcctt ggcgcgcact tcttccgcca attctttgcc gataatgcgt ccgcattctt
    27961 caaagctgat gttttcatcg tgatcgccga ctgcggcttt ggtcgatggc gtaaaaatca
    28021 cttcaggcag ttgttgcgct tcctgcatac cttcgggcag ccgaataccg caaaccgagc
    28081 cggttttttg ataatctttc caaccgctgc ctgccagata accgcgcaca atcgcctcta
    28141 ctttcaccgg agtgagcttt ttagccacga cggcgcgttt ttctaaaact ttggcttcgt
    28201 tttcaggcaa aacatcgtaa accgtttgac cggtaaagtg gttgggcata atatgcgcca
    28261 gttttttaaa ccaaaaattg gaaatctgcg tcagaatctc ccctttgctc ggaatcgggt
    28321 cgtccaaaat cacatcaaac gcggacaggc ggtcggaagc gaccatcagc atacgtttat
    28381 cgtcgatttc atataaatcg cgcacttttc cagaatagat ctttaccaaa ccaatctcac
    28441 tcatttcgcc ccccctgaaa atatcttgaa aataccgacc cgacacccga caggtttgaa
    28501 tcgcaaaccg atattctagc cgaagtcggc gcaaaacaat acccatggca caaaaagcca
    28561 acccgtcaac cgtcggcaaa gttttggcac tataataccg acagcaagtc ctacaataca
    28621 cttttaccaa aggaaatacc tcatgagaat cctattgaca ggctcgaaaa gccaactggc
    28681 acgctgcctg cgcgaccgtc ttccggaaga ctgggaaacc attgcgacgg attccgcatc
    28741 cctagacatt accgatgccg atgccgtctg caacatggtc aaaagtttcc aacccgacgc
    28801 cattgtcaac acggctgcct atactgccgt cgacaaagcg gaaggcgatg cggcagcggc
    28861 atttgccgtc aatgcttccg ccgtttacaa ccttgccttg gcagcacatc gcgcccatgc
    28921 ccgattcatc cacatctcaa ccgactatat ctttgacggt aaagggaaaa ggccctatca
    28981 ggaaagcgac tttaccaatc cttccaatgt atacggacaa tccaaaaccg ccggcgaact
    29041 gctcgcactg tctgccaatc ccgacagcct tatcctgcgg acttcttggc tgtttagcga
    29101 atacggggac aactttgtcc gcaccatgct gaaccttgcg cgggaacgtt ccccgctgtc
    29161 cgccgtccac aaccaaatcg gctgcccgac ctatgccggc gacctggctg ccgccatcat
    29221 ccgccttttg cagcaaacgt cgcccgtgcg cggcatttac cactacgccg gcggcaaatc
    29281 cgtatcctgg tacgaatttg cccaacatat tttccaagcg gcatcgcaac agcagacatc
    29341 cttccccgtt cccgaattga ctgccgtttc agacaaggaa tatccgaccg ccgcccccag
    29401 gcccgcatac agcattttgg actgccgcaa aatcgaaaac gacttcggca tcaaaccgtc
    29461 agactggcag aaagcccttg cacaggtcgt ttccaagctg ctctctagtg ggtactagat
    29521 ataatgcagc atccgataaa gtaaagcccc gaagtggcag cttcggggct tatcggatag
    29581 aaaggaggct tgaagaaatg gcgaaagtta ttttattcct gtttcttttg ttagtttgct
    29641 cgccagccta ctaatgaaaa actgaaataa caaggggtgg caaattaccg cgcccctttc
    29701 gccaaccttt cttcgagcca ttttactaca atggttttaa aaatcaagga ttgcctgatg
    29761 ccgcccgccc ctctgtttcc tccgtcaagc accgccttgg cggttttctt atatagtgga
    29821 ttaacaaaaa ccagtactgc gttgcctcgc cttgccgtac tatttgtact gtctgcggct
    29881 tcgtcgcctt gtcctgattt ttgttaatcc actataaaat ctaccgatta cacaaacaca
    29941 tatcatcttt acacaatcat gcttccatca acagtaaaac atgatatgat tgccaacaat
    30001 aacatctcac aataaatttt ctaattttta ttgaaaaaat caataaagta agaattctcc
    30061 caaccgacaa ataaaataaa gaaagggtta atatgcaaca tcgaagaaga ctagcaattt
    30121 accaagcatc caaacgtgct tcctttaccg gcaggtcatc agccccgcaa aaacgtaaag
    30181 aacgttaatt tgaaaaaaat gccgtctgaa gtcctgcttc agacggcatt tttttaccgt
    30241 tcgagaaact gtttcaacct gtcctcatcc aaaaaccagt ggcagatttc ctcatctccc
    30301 gccaacaaaa cgggaaccag ctcattgtat ttttcttcca aaacaggatt ttcatccaca
    30361 tcgaccactt ccagcccgaa cccgtattca ttttgaaaag gtttgagtgc atcgcgcatt
    30421 ttgtggcaca agctgcaata ttcacgaaac atcaaggtca atttcatccg cgtttcctta
    30481 tctgtcaatt tgcacacgcc aaagccttag acgcagcaga atcatggtct atttgggaaa
    30541 aaacaatgtt ttcaaggaag atgatactca agtcctgcca aaaacagcaa aaatgccgtc
    30601 tgaacagttc agacggcatt tccaaaaccg ttttacgctt gaacattgat acccgccgta
    30661 accgtcggtt gcgcattgtg cgccgtcaga aacgaagcgg caagctcccc acagcagccg
    30721 cagcagctcg ctacgcccaa ttgcgactgc aaatcgccca ttgtggtcgc gccggcggcg
    30781 atggtttcct tgatttgatg gtcggtaacg gcattgcaga tgcagacaaa cattttgtgc
    30841 tccgtgtgtt ctcaaactat cgtaccgata gcggctttta ttatcgtatg cgaatataaa
    30901 taaaaacggt tcgcattgca aggtcggtat acacggtttg tcttggtaat tttttatcaa
    30961 gtttgcattt tcgattgatt tcttattaaa ccaaagtaga agcctcagtt tcgggaatgg
    31021 tgttccttca ctacaccctg caaccatacg ttggcaacat gggtataaat ctgcgtcgta
    31081 ttcaaatcgg catgtcccaa catatcctga accacgcgca aatccaagcc gtgccgcacc
    31141 agatgcgtgg caaaggcgtg gcgcaggctg tgcgggctga tgtgcccgat gcctgcctga
    31201 cttgcatatt ctttgacaat catccatgcc aactgacggg aaatgcccgt ctttttctga
    31261 ctgacaaaca atgcgtcgca attcctgcct ttcagcagaa gtgggcgtgc ctccgtataa
    31321 tagcgttcca cccaatacgc cgactcctgc cccatcggga ccatcctctg cttatcaccc
    31381 tttcccagcg cggtaataca gcccctgtcc aaatccacat tgccgaagtt cagcccgacc
    31441 gcctcgctga cgcgcaagcc ggtcgcgtac atcaattcga gcaaagcctt atcccgcaaa
    31501 ccgtgcggcg tgtcggtatc cggggcggca agcagtcggg aaatctgctg ctcggtgatc
    31561 agggtcggaa tattcttgtc gattttgggc ggtttcagca ggcaggtggg attgtccgtc
    31621 cttatgcctt cacgctccat ccatatatac aggcgtttgc acaccgataa tgcgcgcgcc
    31681 tgcgaactcc gttgctctcc gtcaacataa accgccgccg ccaaatccgc ttcgtccgca
    31741 tccttcagcg ttctgcccga ttgggacagg cggcgggcga ttttttccaa atcgcgccgg
    31801 taaccgttta aagtattctg actgagccgc ctgtccaacc acagcgtttc aagcagcctg
    31861 tcgatcaaac cttcttccat accgttccaa acaaatgccg tctgaatctt cttcagacgg
    31921 catggttcac attatcggga aagcgtttcc aatacttcct gcgcgtgacc cgccactttg
    31981 actttccgcc attcatgggc gatttctcca tccttattca agacgaacgt actgcgctcg
    32041 atacctaacg actctttccc gtacagtttc ttcaatttga tgacatcaaa caggcggcac
    32101 actgtttcat ccttgtcact caacagctcg aaccggaaac cctgcttggc gcaaaaattc
    32161 tgatgcgcct ttacgccgtc gcgggaaata ccgaccacgg tataacccaa tgcctcaaac
    32221 tgttccaaac gcgcattgaa atccaagcct tccgtcgtac agccaggcgt actgtctttc
    32281 ggataaaaat acacgaccaa aggcagatgt tctgccgaat gaaaatccgc accgctgctc
    32341 gaaggcaggg taaattcata tttcacatcc atagtcctac tcccgatatt cccattattc
    32401 aaaacggcac gcagacgcac cgccgcaatt gccaaaccaa ccccgattct accgccccaa
    32461 aggacaagga ttcaaccgcc ggaaacatcc aaaccgacac acgacggcat gaaaaatatc
    32521 catgtcaaac cacaaaatat gttccgattt aaaaacagaa tgttataaaa cccaatcccc
    32581 aaaacacaac aagaccgccc gcctacgggc agtctcctgt cagacgacat actttacaga
    32641 tggctgtttt ttcaacaaaa taacgccaat actcaaaaat atggaatcaa aaatgtccat
    32701 ccatactctg aaacgcctgc cctcatcgct gctgctcggt ctctgccttt ccctgccgtc
    32761 agcccacctt tttgccgaca acgacatttt agggcaattt ttagaacaga acatgcttac
    32821 ctcctccgat ccgatagaaa tattcgccga aagcacaata caccccacca acacccaagc
    32881 cattacaggc ggtctgattc tctcctcaca gtctgccctg gtcgtcaaca acaaaaccgg
    32941 acagatactg tatcagaaaa acgccgacag gattatgccc atcgcctcca tttccaaact
    33001 gatgagcgcg atggtcgttt tggatgcaaa cttggacatg aacgaaaccg ttaccattac
    33061 gcccgacgaa atcgaccgca tcaaagggac cggcagccgt cttgccatag gtacggcact
    33121 tacacgcaaa gaactgctgc acctgagcct gatgagcagc gaaaaccgcg ccacccatgc
    33181 attgggcaga acctaccccg gcggcatggg cgcatttgtc gccgccatga accgcaaagc
    33241 ccaaagcctc ggtatgtacg gcagccgctt ttacgaaccg accggactca acttccaaaa
    33301 cgtttccacc gccaaagacc tgagccttat ggtcaacgcc gccgcccaat atccgcaaat
    33361 ccgcaccaac tcgacttcca actacgcctc ggtacagacc aaaaacgggc agcagaacta
    33421 caaaaactcc aatgccctgg tcagagaagg catgtggaac atcgaattgc agaaaaccgg
    33481 ctacatacgc gaagcaggca ggtctatggt tgtcaaagcc aacattcaaa atcaacccgt
    33541 taccatcgta ttgctgaact cgcccacatc cgccacacgc gtcaacgacg cccgcaaaat
    33601 cgaatcgtgg atgctgcagc aacgctcctg acatacaaat gcccggcgga aaaccgccca
    33661 aacacaaaaa aaattggtaa tttttcctat tcaaggctac aattcgacac gcacagggcg
    33721 tctgcccgat ttcacaatca taataacgga gttaaaaata tgacagctaa aggacaaatg
    33781 ttgcaagatc ccttcctgaa cgccctgcgt aaagagcacg ttccggtttc gatttactta
    33841 gttaacggta tcaaattaca aggtcaggtt gagtctttcg atcaatacgt tgttctcctg
    33901 agaaacactt ccgtcaccca aatggtttac aaacacgcca tttccaccat cgtaccggca
    33961 cgctccgtca acctacaaca tgaaaacaga ccccaagccg caccgacttc gaccctcgtc
    34021 caagtggaaa ccgtccagca gcctgccgaa taatccgcac gaagcatgac gtgtcatatc
    34081 tttcaatacc ttaccggaca acggtaaggt atttttattt tcagacagca tttaaaaatg
    34141 ttattgcaaa acatccttcc attcgcccat tgccttttgc ggaaggcact tcccgaaggt
    34201 ggcaatgctt tggacggcac cgccggcaac ggacacgaca cccttttcct cgcacaaacc
    34261 gcaggcatcc gggggaaagt gtgggcattc gacatccagc cgcaagccct gagccgcact
    34321 cgatgccgtc tgaaagaagc gagatacgag aatgtgcaac tcatcttaga cggacacgaa
    34381 aacctgaaac aatatgtttc agaaccacta aatgccgcca ttttcaactt tggctggctg
    34441 cccggcggag ataaaagcct taccacccat acggaaacca gcattcccgc cctttgcgca
    34501 accctctccc tgctgaaaga aggcggtatg cttattgccg tcctctatcc gggacacgaa
    34561 aacggcaaac aggaggcaga agcaatcgaa caatgggcaa aaaacctgcc tcaagaacag
    34621 tttgccgttt tgcgttacgg ctttaccaac cggaaaaaca acccgcccta tctgttggca
    34681 tttgaaaagt tacgtcaaaa ataaatgttt gcggtaaaat aagccgattc caacccttat
    34741 tttgataaga gccgactatg cagtatctga ttgtcaaata cagccaccaa atcttcgtta
    34801 ccgttaccat tttgttgttc aacatccgct ttttcctgct ttggaaaaat cccgaaaaac
    34861 ctttggcggg cttgtggaag gcactgcccc acctcaacga cacgatgctg ctgtttacgg
    34921 gattgtggct gatgaaaatc acccatttct ccccgttcaa cgcgccttgg ctcggcacaa
    34981 aaatcctgct tctgctcgcc tatatcgcat tgggtatgat gatgatgcgc gcccgccccc
    35041 gctcggtcaa attctatgcc gtttacctgc ttgccatttg ttgcgtcgcc tgtatcgttt
    35101 accttgccaa aaccaaagtc ctgccattct gaaacactgc catgaacaac agacattttg
    35161 ccgtcatcgc cttgggcagc aaccttgaca accccgcaca acaaatacgc ggcgcattgg
    35221 acgcgctctc gtcccacccc gacatccggc ttgaacaggc ttcctcactg tatatgaccg
    35281 cgcccgtcgg ttacgacaat cagcccgatt tcgtcaatgc cgtctgcacc gtctccaccg
    35341 atatggacgg catctcgctg ctctctttgc tcaaccgtat cgaggcggat ttcgggcgcg
    35401 aacgcagttt ccgaaacgcg ccgcgcacat tggatttgga catcatcgac tttgacggcc
    35461 tatccagcga cgacccccgc ctcaccctgc cgcatccgcg cgcacacgaa cgcagtttcg
    35521 tcatacgccc tttggcagaa atcctccctg attttgtttt aggaaaacac ggaaaagttg
    35581 ccgaattgtc aaaacaactg ggcaatcaag gtatccgtct tttaccggat aagtaattcc
    35641 gcacgcggat gccgtctgaa tgccggtttc agacggcatt tttcctttgc cgccaacacg
    35701 cgtgcaaaaa aatcgcccct tggaaaaggg gcgcaaaagg aacacaaacc actaccaaaa
    35761 ctttaaatct gcaacattgc ctgccgcata ctgtatccga caggatataa agccctcact
    35821 aaatcgtttc gagaaatcca aacttcttca tcgccgacag aaaatctgcc tttctccggc
    35881 accagctcca acaaaaacgg ttgaaccgcc gtatgcagcc tgtccttaca ccgccccagc
    35941 tttctggaca aggcggacag ggcacgctta tagacactat ccttgcggtc aagttcccgc
    36001 gcactgccga cccagctcaa acgaaggttg cggctttcat cctcaatcag caaaatgcct
    36061 gttttgactt cccccacctc tggctcgcat gaaagcgaaa tataatattt gccgtcgatg
    36121 ttgacatacg ccgcctcatg cacctgacag ttgcagccgg aagtggcaag cagcgaataa
    36181 acagaatccc gatgcagttt caaaatatca agggaaatct gtttgaaaat actttccggc
    36241 atttttccct cctttcgtct gctgcgttgt ttactggatt gactgcatta tacagacatc
    36301 tcccaatctt gcaaatgata attgttccta ttttttaaaa aatttataaa ttattgatag
    36361 atatgtgaat taattttaag atttaaaatc tctttctcaa aaaaacacaa aaatgccgtc
    36421 tgaaacgtgt tcagacggca ttggctgcac aggcaatccg tttaaaattt cacgccctta
    36481 tgcagcgcaa cgatccccgc actcatatta tgatagtcca cactgtcaaa tccggcatcc
    36541 agcatcattt gctttaaagt ttcctgatcg gggtgcatac ggatggattc ggcgagatac
    36601 tggtagctgt ccgcatcttt ggcaatcagt ttgcccatga ccggcagcag cttgaacgaa
    36661 tagaaatcat acgcgccttc gagcggcttg tagattttgg aaaactccaa caccagcaac
    36721 gtgccgccag gtttcaaaac acgatacatc tctttcagcg cggcatcttt atgcgtcatg
    36781 ttccgcaagc cgaacgccac ggaaactaag ttgaaataat tgtcagggaa aggcagtttt
    36841 tccgcatcgg caagcgacac gggcaaaatc atgccttcgt tcaacagacg gtcgcgcccg
    36901 acggtcagca tagaggaatt aatatcggtc agccaaacct cgccttcctt gccgacccgt
    36961 ttcgcccaac cgcgcgacaa atcgcccgta ccgcccgcaa tgtccaacac tttatcgcct
    37021 tttttcaggt gcgccgtgtt gatggtgaaa tgcttccaca ccctgtgcag ccctgccgac
    37081 atgacatcat tcataatgtc gtagtttttg gcgacggagt ggaacacttc cgccactttg
    37141 ccggcttttt catcttcgtt gaccgtgcta aacccgaaat gcgttttttg tccgcccata
    37201 ttctaaccac ctccctgata ccgccctacg gcatcccaaa cttaacaatc tgattgcttt
    37261 tcaaaacgcg acgcgcccgc cgccgccaat ttgtccaaat attcctgcca catcgcatcg
    37321 tatccgtatg ccagtctgtg cagatacgcc caatcgtaaa gaccgctgtc gtgcccgtcc
    37381 gaaaaactga ttttcagcgc gtactgtccg acaggctgca aatccgtaat ttgcacatct
    37441 gccttaccgg tctgcaaaac atcctgtccc acaccgtgtc cgcgcacttc cgcactcggc
    37501 gaatagacgc gtaaaaattc ggcaggcaga tttttaggtt cgtccccata agtcaaaacc
    37561 aacacggtac ggtttttcaa caaacggatt tcaacaggaa tattattcaa aaaacacctc
    37621 ggttcggcat acaatatccg gcaggcgcag gcgtgccttc aaacggcgcg tattttaaca
    37681 gctttatccc gataccgata ttcaaaccca cactttaaac ggcattgata tggacaatct
    37741 cgtatgggac ggcgttcccg acatccgcac actcgaccaa accatccgca aacacgcaca
    37801 cccgctcaac ctgattgtct gcctccccga taatcagatt cccgattttc aaaccgcaca
    37861 agatgcttcg gacgcggaat gccgtctgaa gcaccgtttg gatcaggcaa cgcagtgcct
    37921 ccagttcgac agcatcaacc tcatcgaaca catcctgccc gatgtccgct tctggctggt
    37981 tcccccttca cgcacccgcc gcctgcacga acacttccac cacatttcct ggcagaccga
    38041 agccatcccg caaaccgaaa gcaaacccga caaaccctgg tttgcacttc cacaaacatc
    38101 cgaacggcaa aaaccggaac acatcctcgt tatcggcgcg ggcatatccg gcgcggcaac
    38161 cgcccacgcc ttagcatcac acggcatttc cgttaccgta ttggaagccc gaaaagccgc
    38221 ccaagccgcc agcggcaacc gccaagggct gctctacgcc aaaatctcgc cgcacgacac
    38281 cgaacaaacc gaactgctgc ttgccggcta cggctacacc aaacgcctgc tcggacacat
    38341 cctgcccgaa tccgaaacct ggggcggcaa cggcatcatc cacctcaatt acagccgcac
    38401 cgaacaacaa cgcaatcacg aattgggttt gcaaaaacac cataaccacc tctaccgcag
    38461 catcacgccg gcagaagccg aaaaaatcgc cggcatcccg ctgaacacgc cctacgccga
    38521 accattatgc ggactgtttt ggcagtacgg cgtatggctc aatccacccg cattcgtccg
    38581 caccctcctc aaccatccgc tcattggact acacgaagac acaccgttaa ccgacatttc
    38641 ccacgacggg gaaaagtgga ttgcaagcac gccaaacggc atattttccg ccacacacat
    38701 catctactgc accggcgcgc acagccctcg cctgcccgaa accaacctcg ccgccctgcc
    38761 tctcaggcaa atacgcggac aaaccggcct cacaccgtcc accccgtttt ccgaacaact
    38821 gcgttgcgcc gtttcaggcg aaagctacat cagcccgtcg tggcacggac tgcactgcta
    38881 cggcgcgagt tttattccca acagcagcca taccggatgg aacgaagccg aagaagcctc
    38941 aaaccgccaa gcattggcac accttaaccc cgccctttcc gaatcattgt ttgccgccaa
    39001 cccaaacccc caaaaacacc aagggcacgc cgccatacgc tgcgacagcc ccgaccacct
    39061 tcccctagtc ggcgcactcg gcgacattgc cgccatgcgg cagacctaca ccaaactcgc
    39121 gctggacaaa aactaccgca tcgacacccc atgcccatac ctgcctaatg cctacgtcaa
    39181 caccgcgcac ggcacccgcg gactcgccac cgcccccatc tgcgccgccg ccattgcagc
    39241 ccaaatccta ggcttgcccc atcccttttc acaacgcctg cgccacgccc tacaccccaa
    39301 ccgcaccatc atccgcgcca tcgtcagaag gaaggatcta accccttaag cctttgccgc
    39361 atgccgtccc gaccacagaa aatcatattc aaataaacat accaatcctg acggtaaaat
    39421 atcgaaaaca cccataacgt tttcttccca aaataaggag taaggaatgc ctaacaaatt
    39481 gcctttgctg tccaagatga atgttccgta acattcgaag acttccttaa ccctgccagc
    39541 cgtttcggtt tctgtacccc ctgttagata aggaaggaat gaaaaagccc tgttgattgt
    39601 atgaagtaaa gggcgttaat ttttgttaat cttcccttct caggaactca atatataagg
    39661 atgtt
//
LOCUS       DAWHXS010000004        39489 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWHXS010000004 DAWHXS010000000
VERSION     DAWHXS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39489
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.004"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            60..371
                     /gene="rpsJ"
                     /locus_tag="WIE22_000134"
     CDS             60..371
                     /gene="rpsJ"
                     /locus_tag="WIE22_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ4126287.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            492..816
                     /locus_tag="WIE22_000135"
                     /pseudo
     CDS             492..816
                     /locus_tag="WIE22_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1060..1704
                     /gene="rplC"
                     /locus_tag="WIE22_000136"
     CDS             1060..1704
                     /gene="rplC"
                     /locus_tag="WIE22_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ4126288.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1704..2324
                     /gene="rplD"
                     /locus_tag="WIE22_000137"
     CDS             1704..2324
                     /gene="rplD"
                     /locus_tag="WIE22_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ4126289.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2321..2635
                     /gene="rplW"
                     /locus_tag="WIE22_000138"
     CDS             2321..2635
                     /gene="rplW"
                     /locus_tag="WIE22_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ4126290.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2641..3474
                     /gene="rplB"
                     /locus_tag="WIE22_000139"
     CDS             2641..3474
                     /gene="rplB"
                     /locus_tag="WIE22_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ4126291.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3481..3759
                     /gene="rpsS"
                     /locus_tag="WIE22_000140"
     CDS             3481..3759
                     /gene="rpsS"
                     /locus_tag="WIE22_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ4126292.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3768..4097
                     /gene="rplV"
                     /locus_tag="WIE22_000141"
     CDS             3768..4097
                     /gene="rplV"
                     /locus_tag="WIE22_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ4126293.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4107..4799
                     /gene="rpsC"
                     /locus_tag="WIE22_000142"
     CDS             4107..4799
                     /gene="rpsC"
                     /locus_tag="WIE22_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ4126294.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4783..5199
                     /gene="rplP"
                     /locus_tag="WIE22_000143"
     CDS             4783..5199
                     /gene="rplP"
                     /locus_tag="WIE22_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ4126295.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5199..5390
                     /gene="rpmC"
                     /locus_tag="WIE22_000144"
     CDS             5199..5390
                     /gene="rpmC"
                     /locus_tag="WIE22_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ4126296.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5390..5653
                     /gene="rpsQ"
                     /locus_tag="WIE22_000145"
     CDS             5390..5653
                     /gene="rpsQ"
                     /locus_tag="WIE22_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ4126297.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5875..6243
                     /gene="rplN"
                     /locus_tag="WIE22_000146"
     CDS             5875..6243
                     /gene="rplN"
                     /locus_tag="WIE22_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ4126298.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6255..6578
                     /gene="rplX"
                     /locus_tag="WIE22_000147"
     CDS             6255..6578
                     /gene="rplX"
                     /locus_tag="WIE22_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ4126299.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6588..7127
                     /gene="rplE"
                     /locus_tag="WIE22_000148"
     CDS             6588..7127
                     /gene="rplE"
                     /locus_tag="WIE22_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ4126300.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7130..7435
                     /gene="rpsN"
                     /locus_tag="WIE22_000149"
     CDS             7130..7435
                     /gene="rpsN"
                     /locus_tag="WIE22_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ4126301.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7451..7843
                     /gene="rpsH"
                     /locus_tag="WIE22_000150"
     CDS             7451..7843
                     /gene="rpsH"
                     /locus_tag="WIE22_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ4126302.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYADRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7857..8390
                     /gene="rplF"
                     /locus_tag="WIE22_000151"
     CDS             7857..8390
                     /gene="rplF"
                     /locus_tag="WIE22_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ4126303.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8404..8757
                     /gene="rplR"
                     /locus_tag="WIE22_000152"
     CDS             8404..8757
                     /gene="rplR"
                     /locus_tag="WIE22_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ4126304.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8776..9294
                     /gene="rpsE"
                     /locus_tag="WIE22_000153"
     CDS             8776..9294
                     /gene="rpsE"
                     /locus_tag="WIE22_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ4126305.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9287..9472
                     /gene="rpmD"
                     /locus_tag="WIE22_000154"
     CDS             9287..9472
                     /gene="rpmD"
                     /locus_tag="WIE22_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ4126306.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            9474..9908
                     /gene="rplO"
                     /locus_tag="WIE22_000155"
     CDS             9474..9908
                     /gene="rplO"
                     /locus_tag="WIE22_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ4126307.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9920..11230
                     /gene="secY"
                     /locus_tag="WIE22_000156"
     CDS             9920..11230
                     /gene="secY"
                     /locus_tag="WIE22_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ4126308.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11235..11453
                     /gene="infA"
                     /locus_tag="WIE22_000157"
     CDS             11235..11453
                     /gene="infA"
                     /locus_tag="WIE22_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ4126309.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11474..11587
                     /gene="rpmJ"
                     /locus_tag="WIE22_000158"
     CDS             11474..11587
                     /gene="rpmJ"
                     /locus_tag="WIE22_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ4126310.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            11653..12015
                     /gene="rpsM"
                     /locus_tag="WIE22_000159"
     CDS             11653..12015
                     /gene="rpsM"
                     /locus_tag="WIE22_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ4126311.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12035..12430
                     /gene="rpsK"
                     /locus_tag="WIE22_000160"
     CDS             12035..12430
                     /gene="rpsK"
                     /locus_tag="WIE22_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ4126312.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12450..13070
                     /gene="rpsD"
                     /locus_tag="WIE22_000161"
     CDS             12450..13070
                     /gene="rpsD"
                     /locus_tag="WIE22_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ4126313.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13096..14082
                     /gene="rpoA"
                     /locus_tag="WIE22_000162"
     CDS             13096..14082
                     /gene="rpoA"
                     /locus_tag="WIE22_000162"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ4126314.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14106..14474
                     /gene="rplQ"
                     /locus_tag="WIE22_000163"
     CDS             14106..14474
                     /gene="rplQ"
                     /locus_tag="WIE22_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ4126315.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14621..15334
                     /gene="minC"
                     /locus_tag="WIE22_000164"
     CDS             14621..15334
                     /gene="minC"
                     /locus_tag="WIE22_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ4126316.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSDERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15363..16178
                     /gene="minD"
                     /locus_tag="WIE22_000165"
     CDS             15363..16178
                     /gene="minD"
                     /locus_tag="WIE22_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ4126317.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16182..16445
                     /gene="minE"
                     /locus_tag="WIE22_000166"
     CDS             16182..16445
                     /gene="minE"
                     /locus_tag="WIE22_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ4126318.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVDVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16449..17369
                     /gene="oxyR"
                     /locus_tag="WIE22_000167"
     CDS             16449..17369
                     /gene="oxyR"
                     /locus_tag="WIE22_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ4126319.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(17366..17557)
                     /locus_tag="WIE22_000168"
     CDS             complement(17366..17557)
                     /locus_tag="WIE22_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126320.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(17699..20536)
                     /locus_tag="WIE22_000169"
     CDS             complement(17699..20536)
                     /locus_tag="WIE22_000169"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ4126321.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHTFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(20633..21442)
                     /gene="zupT"
                     /locus_tag="WIE22_000170"
     CDS             complement(20633..21442)
                     /gene="zupT"
                     /locus_tag="WIE22_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ4126322.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFHF"
     gene            complement(21623..22879)
                     /locus_tag="WIE22_000171"
     CDS             complement(21623..22879)
                     /locus_tag="WIE22_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ4126323.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETKTGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTHFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(22958..24349)
                     /locus_tag="WIE22_000172"
     CDS             complement(22958..24349)
                     /locus_tag="WIE22_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ4126324.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVLLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWITAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(24706..25482)
                     /gene="lpxA"
                     /locus_tag="WIE22_000173"
     CDS             complement(24706..25482)
                     /gene="lpxA"
                     /locus_tag="WIE22_000173"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ4126325.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKNVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSTRGIIR"
     gene            complement(25525..26205)
                     /locus_tag="WIE22_000174"
     CDS             complement(25525..26205)
                     /locus_tag="WIE22_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ4126326.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDVTGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(26412..26861)
                     /gene="fabZ"
                     /locus_tag="WIE22_000175"
     CDS             complement(26412..26861)
                     /gene="fabZ"
                     /locus_tag="WIE22_000175"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ4126327.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(26895..27941)
                     /gene="lpxD"
                     /locus_tag="WIE22_000176"
     CDS             complement(26895..27941)
                     /gene="lpxD"
                     /locus_tag="WIE22_000176"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ4126328.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(27974..28474)
                     /locus_tag="WIE22_000177"
     CDS             complement(27974..28474)
                     /locus_tag="WIE22_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ4126329.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKVQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(28540..30933)
                     /gene="bamA"
                     /locus_tag="WIE22_000178"
     CDS             complement(28540..30933)
                     /gene="bamA"
                     /locus_tag="WIE22_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ4126330.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKQYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(30990..32330)
                     /gene="rseP"
                     /locus_tag="WIE22_000179"
     CDS             complement(30990..32330)
                     /gene="rseP"
                     /locus_tag="WIE22_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ4126331.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(32365..33600)
                     /locus_tag="WIE22_000180"
     CDS             complement(32365..33600)
                     /locus_tag="WIE22_000180"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ4126332.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(33605..34402)
                     /locus_tag="WIE22_000181"
     CDS             complement(33605..34402)
                     /locus_tag="WIE22_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ4126333.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(34405..35151)
                     /locus_tag="WIE22_000182"
     CDS             complement(34405..35151)
                     /locus_tag="WIE22_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ4126334.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(35207..35764)
                     /gene="frr"
                     /locus_tag="WIE22_000183"
     CDS             complement(35207..35764)
                     /gene="frr"
                     /locus_tag="WIE22_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ4126335.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(35944..37071)
                     /locus_tag="WIE22_000184"
     CDS             complement(35944..37071)
                     /locus_tag="WIE22_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ4126336.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(37145..37220)
                     /locus_tag="WIE22_000185"
     tRNA            complement(37145..37220)
                     /locus_tag="WIE22_000185"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37185..37187),aa:Thr,seq:cgt)
     gene            37325..37810
                     /locus_tag="WIE22_000186"
     CDS             37325..37810
                     /locus_tag="WIE22_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126337.1"
                     /translation="MYVNEKYPYAALFAGLVFLTLPFALSVHDALAFAFGRAGLLVSV
                     SDGEFGWRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            37906..38529
                     /gene="rsmG"
                     /locus_tag="WIE22_000187"
     CDS             37906..38529
                     /gene="rsmG"
                     /locus_tag="WIE22_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ4126338.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            38628..39401
                     /locus_tag="WIE22_000188"
     CDS             38628..39401
                     /locus_tag="WIE22_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ4126339.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSATVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
BASE COUNT         9299 a         8962 c        10801 g        10427 t
ORIGIN      
        1 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aattgaagga tattgataaa
       61 tggcaaacca aaaaatccgt atccgcctga aagcttatga ttacgccctg attgaccgtt
      121 ctgcacaaga aatcgttgaa actgcaaaac gtaccggtgc cgttgtaaaa ggtccgattc
      181 cgttgccgac caaaatcgaa cgtttcaaca ttctgcgttc tccgcacgtg aacaaaactt
      241 cccgtgaaca attggaaatc cgcacccact tgcgcctgat ggacatcgtg gattggaccg
      301 ataaaactac cgatgcgctg atgaagctgg atttgccggc cggtgttgat gtagaaatca
      361 aagtccaata attcggacta ttgaaaatcc ccaagcaatc aatgcttggg gattttttat
      421 gttatgccga gacctttgca aaattcccca aaatccccta aattcccacc aagacattta
      481 ggggatttct catgagcacc ttcttccaac aaaccgccca agccatgatt gccaaacaca
      541 tcgaccgttt cccgctattg aagttggacc aggtgattga ttggcagccg atcgaacaat
      601 acctgaaccg tcaaaaaccc gttacctccg agattaccgc ggccgtcccg cctacccgct
      661 gttgtccatg ttcaaagccg tcctgctcgg acaatggcac agcctctccg atcccgaact
      721 cgaacacagc ctcatcaccc gcatcgattt caacctgttt tgccgctttg acgaactgag
      781 catccccgat tacagtcatc aaccatattc cggtttgtcg gagaaagatg catacgctgt
      841 gatgaccgga taccgaccct ttaaaagagt ctgaccctat gccgtctgaa aattcaaaac
      901 gcttcagacg gcatattgaa gatatttctg atatttctgt tgatatttct ttaacttgtc
      961 agatataatg ccgagcttgg tacatttgtg ccaagtttaa ctttgtctga aagacaggcc
     1021 aatcgtagcc tgtcccttta ctttaaaagg aaaataatca tgactttagg tctggttgga
     1081 cgcaaagttg gtatgacccg cgtgttcgac gaacagggtg tttctgttcc ggtaaccgtt
     1141 ttggatatgt ctgccaaccg cgttacacaa gtaaaatcca aagatactga cggctatact
     1201 gccgttcaag ttacctttgg tcagaaaaaa gccaatcgtg tcaacaaagc cgaagccggg
     1261 cactttgcaa aagcaggtgt tgaagccggt cgcggtttga ttgagtttgc tttgactgaa
     1321 gaaaaactgg ctgaattgaa agctggtgac gaaatcaccg tttctatgtt tgaagtcggt
     1381 caactggtcg atgtaaccgg tacctctaaa ggtaaaggtt tctccggcac gattaaacgt
     1441 cataacttcg gcgcccaacg tacttcccac ggtaactccc gctctcaccg cgttccaggt
     1501 tctatcggta tggcacaaga cccgggtcgc gtgttccccg gtaagcgcat ggccggccaa
     1561 tacggcaaca ccaaggcaac tgttcaaaaa ttggaagttg tccgtgttga tgcagaacgc
     1621 caactgctgt tggttaaggg tgctgttccg ggtgcggtca acagcgatgt tgtagttcgt
     1681 cccagcgtga aagtaggtgc gtaatggaat tgaaagtaat tgacgctaaa ggacaagttt
     1741 caggcagcct gtctgtttct gatgctttgt tcgcccgcga atacaatgaa gcgttggttc
     1801 atcagctggt aaatgcctac ttggcaaacg cccgctccgg taaccgcgct caaaaaaccc
     1861 gtgccgaagt aaaacactca accaaaaaac catggcgtca aaaaggtacc ggccgtgccc
     1921 gttccggtat gacttcttct ccgctgtggc gtaaaggtgg tcgcgcgttc ccgaacaaac
     1981 ccgacgaaaa cttcactcaa aaagtaaacc gcaaaatgta ccgtgccggt atggcgacta
     2041 ttctgtccca attgactcgt gacgagcgtt tgtttgcgat tgaggcgttg actgccgaaa
     2101 ctcctaaaac caaagttttt gctgaacaag tgaaaaatct gggcctagag caagtgttgt
     2161 ttgtaaccaa acagctcgac gagaatgttt acttggcttc acgcaacttg ccaaacgtgt
     2221 tggttttgga agctcaacaa gttgatcctt acagcttgct gcgttacaaa aaagtaatca
     2281 tcactaaaga tgcagttgca caattagagg agcaatgggt atgaatcaac aacgtttgac
     2341 tcaagtgatt ttggcaccta tcgtttctga aaaaagcaac gtattggctg aaaaacgcaa
     2401 ccaaatgacg tttaaagttt tggcaaatgc aaccaaacct gaaatcaaag cggctgttga
     2461 gctgctgttt ggtgttcaag ttgcttctgt aactaccgtt acaactaaag gcaaaactaa
     2521 gcgttttggt cgtactttgg gccgccgcag cgatgttaaa aaagcttatg taagcttggc
     2581 tgccggtcaa gagttggatt tggaagccgc tgctgcagct gcagataagg aataaacaaa
     2641 atggcaatcg ttaaaatgaa gccgacctct gcaggccgtc gcggcatggt tcgcgtggta
     2701 acagaaggtt tgcacaaagg tgcgccttat gcacctttgc tcgaaaagaa aaattctact
     2761 gccggtcgta acagtaatgg tcatatcacc acccgtcaca aaggcggcgg tcataaacac
     2821 cattaccgtg ttgtagactt taaacgtaac aaagacggca ttcctgctaa ggtagagcgt
     2881 atcgaatacg atcctaaccg tactgccttc atcgcactgt tgtgctatgc agacggcgag
     2941 cgtcgttaca tcatcgctcc tcgcggtatt caagtcggtg ccgtattggt ttccggtgct
     3001 gaagctgcca tcaaagtagg caacaccctg ccgatccgca acattcccgt tggtacgact
     3061 atccactgta tcgaaatgaa acccggtaaa ggtgctcaaa tcgcacgttc tgccggtgct
     3121 tctgcggtat tgctggctaa agaaggcgcg tacgctcaag tccgtctgcg ctctggcgaa
     3181 gttcgtaaaa tcaacgtaaa ttgccgtgcg accatcggtg aagttggtaa cgaagagcaa
     3241 agcctgaaaa aaatcggtaa agccggtgcc aaccgttggc gcggtattcg tccgaccgta
     3301 cgcggtgttg tcatgaatcc tgtcgatcac ccgcatggtg gtggtgaagg ccgtacgggc
     3361 gaggcccgcg aaccggtcag cccatggggt actcctgcta aaggctaccg cactcgtaat
     3421 aacaaacgca cggataacat gattgttcgt cgccgttact caaataaagg ttaatttagt
     3481 atggctcgtt cattgaaaaa aggcccatat gtagacctgc atttgctgaa aaaagtagat
     3541 gctgctcgcg caagcaacga caaacgcccg attaaaacct ggtctcgtcg ttctaccatt
     3601 ctgcctgatt ttatcggtct gaccattgct gtgcacaacg gccgcaccca tgtgcctgtg
     3661 tttatcagcg acaatatggt tggtcataaa ttaggcgaat tctcattgac ccgtaccttt
     3721 aaaggccact tggccgataa aaaggctaaa aagaaataag gtgaatcatg agagtaaatg
     3781 cacaacataa aaatgcccgt atctctgctc aaaaggctcg tttggtagct gatttgattc
     3841 gtggtaaaga cgttgcccaa gctttgaata ttttggcttt cagtcctaaa aaaggtgccg
     3901 agctgattaa aaaagtattg gagtcagcta ttgctaatgc cgagcacaat aacggtgcgg
     3961 acattgatga actgaaagtg gtaactatct ttgttgacaa aggcccaagc ttgaaacgtt
     4021 ttcaagctcg cgccaaaggt cgcggtaacc gcatcgaaaa acaaacttgt catatcaatg
     4081 tgacagtggg taactaagga aaagctatgg gacaaaagat taaccctaca ggctttcgcc
     4141 tggcggtaac taaagactgg gcttcaaaat ggtttgctaa aagcaccgac ttttctactg
     4201 ttttgaagca ggatatcgat gttcgcaatt atttgcgtca aaaattggcc aatgcttcgg
     4261 ttggtcgagt ggttattgaa cgccctgcaa aatctgcacg cattaccatt cactccgctc
     4321 gtccgggtgt ggttatcggt aaaaaaggtg aggatatcga ggttttgaaa cgtgacttgc
     4381 aagtcttgat gggtgtacct gttcatgtaa atattgaaga gattcgccgt cctgagttgg
     4441 atgctcaaat tattgctgac ggtattgccc agcagttgga aaagcgcgtt caattccgtc
     4501 gtgctatgaa acgagcaatg caaaatgcaa tgcgttctgg tgctaaaggc attaagatta
     4561 tgacttcagg ccgtctgaat ggtgcggata ttgcccgtag cgaatggtat cgtgaaggtc
     4621 gtgtgccact gcatacttta cgtgcaaatg tagattatgc aaccagcgaa gcgcacacca
     4681 catatggtgt attgggtctg aaagtttggg tttatacgga aggcaatatt aaatcttcca
     4741 aacctgaaca tgagagtaaa caaagaaagg caggtagacg taatgctgca gccaactaga
     4801 ctgaaatacc gtaagcaaca aaagggtcgc aatactggca tcgctactcg cggtaataag
     4861 gtaagtttcg gtgagttcgg cttgaaagcc gtaggtcgtg gtcgtttgac tgcccgtcaa
     4921 atcgaagctg ctcgtcgtgc aatgacccgt catatcaaac gtggtggtcg tatttggatt
     4981 cgtgtattcc ctgataaacc gattactgaa aagcctattc aagttcgtat gggtggcggt
     5041 aaaggtaacg tggaatatta cattgccgaa attaaaccag gtaaagtgtt gtatgaaatg
     5101 gatggcgttc cagaggaact ggctcgtgaa gcattcgagt tggctgctgc caaattgcct
     5161 attcctacaa cctttgtagt aagacaggtg ggtcaataat gaaagcaaat gaattgaaag
     5221 acaaatccgt tgagcagttg aatgcagatt tgttggactt gttgaaagct cagtttggct
     5281 tacgtatgca aaacgctacc ggtcaattag gcaaaccaag tgaattgaaa cgtgtacgtc
     5341 gcgatattgc tcgtattaaa accgttttaa ctgaaaaagg tgctaagtaa tgagcgaaac
     5401 taaaaatgtt cgtactttgc aaggcaaagt agtaagcgac aaaatggata aaaccgtaac
     5461 agtattggtt gagcgtaaag taaaacatcc gctgtatggt aagattattc ggttatctac
     5521 taaaatccat gcccatgatg aaaataatca atatggaatt ggtgatgtgg ttgttatatc
     5581 ggaatcccgt ccattgtcaa aaactaaatc ttgggttgtc agtgagctgg ttgagaaagc
     5641 acgttctatt taagaattaa agcaacgtgc ttggaatggg aaacgaagta ttgcagcaaa
     5701 tttaatttgc gtgtaaactt cgtttcctgt ctttcagttt cttctggaag tttcttccct
     5761 ttcggggtcc aagactggtt tacttgaacc gcaaggtttc atttaataag cagcggcttt
     5821 gctgtaagtt atctgaaagt ggtaaattaa gttggttaat ttaaaggtaa taacatgatt
     5881 caaatgcaga ccatcttaga tgtggctgat aactctggtg cgcgtcgcgt aatgtgtatc
     5941 aaggtattgg gcggatctaa gcgtcgctac gcttctgttg gcgatattat taaagtggca
     6001 gttaaagatg cggctccgcg tggccgtgtc aaaaaaggcg atgtatataa tgcggtagtt
     6061 gttcgtactg ctaagggtgt acgtcgtcct gatggtgcgt taattaaatt cgataacaat
     6121 gccgccgtgt tactgaataa taaacttgaa cctttgggta ctcgtatctt tggtccggta
     6181 acccgtgaat tgcgtactga gcgatttatg aaaatcgttt cattggcacc tgaagtatta
     6241 taaggaatgg cacgatgaat aaaatcatta aaggcgatag ggttgtagta attgctggta
     6301 aggataaagg taagcagggt caagtagttc gagtgttggg tgataaagtt gttgttgagg
     6361 gcgttaatgt tgtaaaacgc catcaaaaac ctaatccaat gcgtggcatt gagggcggta
     6421 ttattactaa agaaatgcct ttggatattt ctaatatcgc aatcctgaat ccggaaacta
     6481 ataaagcgga ccgtgttggt attaagctga ttgaaaatga aggcaaagtt aaacgcgttc
     6541 gtttcttcaa atcaaatggc tctatcattg gggcataagg agataacatg gctcggttga
     6601 gagagtttta taaagagaca gttgttcctg aattggttaa acaatttggt tacaaatcag
     6661 taatggaagt cccgcgtatt gaaaaaatca ccttgaatat gggtgtgggt gaggctgttg
     6721 ctgacaaaaa agttatggaa catgctgttt ccgatttaga gaaaattgcc ggtcaaaaac
     6781 cggttgttac tgttgcccgt aaatctatcg caggttttaa aatccgtgat aactatccgg
     6841 ttggttgcaa agtaacattg cgtcgtgatc aaatgtttga attcttggat cgtttgatta
     6901 ctattgcatt acctcgcgta cgtgacttcc gtggtgtgag cggtaaatca tttgatggcc
     6961 gtggcaatta caatatgggt gttcgtgagc aaattatttt tccggaaatt gaatacgata
     7021 aaattgatgc tttgcgtggt ttgaatatta ctattactac tacagcaaaa accgatgagg
     7081 aagcgaaagc tttattgtca ttgtttaaat ttccgttcaa aggataatca tggctaagaa
     7141 agcacttatt aatcgtaatc tgaaacgtca agctttggct aaaaaatatg cggctaaacg
     7201 cgcggcaatt aaagcggtaa tcaatgattc gaatgcaact gaggaagagc gttttgaggc
     7261 tcgtttgagg tttcaatcca ttcctcgtaa tgcggcacct gtgcgtcaac gtcgtcgttg
     7321 tgctttgaca ggtcgccctc gtggtacttt ccgtaaattt ggtttgggtc gtattaaaat
     7381 ccgtgaaatc gccatgcgtg gcgaaattcc gggtgttgtt aaagccagct ggtaatagga
     7441 gtaattaaga atgagtatgc atgatcctat ttccgatatg ttgactcgta tccgcaatgc
     7501 gcaacgtgct aataaagcag cggttgcaat gccttcttca aaattaaagt gtgctattgc
     7561 aaaggtattg aaagaagaag gatatattga ggacttcgca gtttcatctg acgtaaagtc
     7621 tatattggaa attcaattaa aatactatgc agatcgtcct gtaattgaac aaatcaagcg
     7681 tgtatctcgc cccggtttgc gtatttataa agcgtctagt gagattccaa gtgttatgaa
     7741 tggcttgggt attgctattg ttagtacttc taaaggtgta atgactgatc gtaaagcacg
     7801 ttctcaaggt gttggtggtg agttgttatg cattgtagcc tagtggagga aaagaaatgt
     7861 cacgtgtcgc aaaaaaccca gtgactgttc ccgctggtgt agaagtaaaa tttggaacag
     7921 aggcattagt tattaagggt aagaacggtg aattgtcttt tcctttgcat tctgatgtag
     7981 ccattgaatt taatgatggc aaattggctt ttgttgcgaa taacagcagt aaacaagcaa
     8041 atgcaatgtc tggtactgct cgcgcattag tcagcaatat ggttaaaggt gtttcagaag
     8101 gttttgagaa aagattgcaa ttgataggtg tgggttatcg tgctcaagca caaggtaaaa
     8161 tcttgaatct gtctttgggt ttttctcatc cgatcgtata tgaaatgcct gaaggtgtct
     8221 ccgttcaaac tcctagccaa acagagattg ttttaaccgg ctcggataaa caagttgttg
     8281 gtcaagttgc tgctgagatt cgtgcgttcc gtgctcctga gccttataaa ggtaaaggtg
     8341 ttcgctatgt aggagaagta gtggtaatga aagaagccaa gaaaaaataa ttgaggttca
     8401 ctaatggata aacatacaac ccgactccgt cgtgcacgca aaacccgtgc tcgtattgcg
     8461 gacttgaaaa tggtaagatt atgtgtgttc cgaagcaata atcatattta tgctcaagta
     8521 attagtgctg aaggtgataa agtattggct caagcctcta cattggaagc tgaggtgcgc
     8581 ggtagtctga aatctggaag caatgttgaa gcagctgcaa tagttggtaa acgtatcgct
     8641 gagaaggcta aagcagcagg tgtagaaaag gttgcttttg atcgttcagg tttccaatat
     8701 cacggtcgtg tgaaggcttt ggctgaagct gctcgtgaaa atggtttaag cttctaaata
     8761 tttggagact ttcagatggc aaaacatgaa attgaagaac gcggtgacgg tctgattgaa
     8821 aagatggtcg cagttaaccg tgtaaccaaa gtagttaaag gtggtcgcat tatggctttc
     8881 tctgcgctaa ctgttgttgg tgatggagat ggtcgtattg gtatgggtaa aggtaagtca
     8941 aaagaagtgc ctgttgcagt ccaaaaggcg atggatcaag cacgacgctc tatgattaag
     9001 gtaccattaa aaaatggtac gattcatcat gaggttattg gtcgacatgg tgctactaaa
     9061 gtatttatgc agcctgctaa agagggtagt ggcgtaaaag ccggtggacc tatgcgtttg
     9121 gtttttgatg ctatgggcat tcataatatc tccgccaaag tgcacggatc tactaaccca
     9181 tataatatcg tacgtgcaac attagatggt ttgtctaagt tgcatactcc tgctgatatc
     9241 gcagccaaac gtggcttgac agtggaagac attttgggag ttaaccatgg ctgaacaaaa
     9301 aaagattagg gttacattgg ttaaaagcct gattggtaca attgaatctc atcgtgcatg
     9361 tgcacgcggt ttaggtttgc gtcgtcgcga gcatacggta gaggttttag atacccctga
     9421 aaaccgtggt atgattaata aaatcagcta cttgttgaaa gtggagtctt gatatgtttt
     9481 tgaatacaat tcaacctgct gttggtgcta cgcatgctgg tcgtcgtgtt ggacgcggta
     9541 ttggtagtgg tcttggcaaa acgggtggtc gtggtcataa aggtcaaaag agccggtctg
     9601 gtgggtttca taaggtgggt ttcgagggtg gtcaaatgcc cttgcaacga cgcctcccta
     9661 aaagaggttt taaatcttta acagcatcag ctaatgcaca gcttcgttta agtgaactgg
     9721 aatcaattgc tgttaatgag attgatattt tggtcttaaa gcaagcgggt ctgattgcat
     9781 ctacagtctc taatgttaaa gttattgctt ctggtgaaat ttctaaggca gttgctttga
     9841 agggtattaa agttaccaaa ggtgcgagag ctgctatcga ggctgttggt ggtaagattg
     9901 aaatgtaagg tttaatattg tggctaatca acaaacgtca tcaggttcat ccaaatttgg
     9961 agatcttaag aaacgtcttt tgtttctatt tggagcattg attgtttttc gaattggtgc
    10021 ccatataccc gtacctggag ttgatgctgt tgctttagct aaattatacg aaagcgctgg
    10081 aaacggcatc ctgggaatat tgaatatgtt ttccggtggg tcgttagagc gctttagtat
    10141 atttgcaata ggaattatgc catatatttc agcttctatt attgtacagc tcgcttctga
    10201 aattttgcca tcattgaagg ctttaaaaaa agaaggggag gctggtagaa aggtaattac
    10261 gaaatatact aggtatggta ctgttttgtt agcaattctt caaagtctag gtgttgcatc
    10321 tttcgtattt cagcaaggaa ttgttgtaac aagttcattt gagtttcatg tttccacggt
    10381 agtttctttg gtaacgggaa ccatgtttct tatgtggctt ggggagcaaa ttactgaaag
    10441 gggtatcggg aacggtattt ctttaatcat tacggcaggt attgcttcag gtattccttc
    10501 gggtattgca aagctggtta cactgacgaa ccaaggttct atgagcatgc ttacggcgtt
    10561 gtttattgta tttggtgcct tattattaat ttatttggtt gtatactttg aaagtgcaca
    10621 gcggaagatt cctattcatt atgcaaaacg ccagtttaat ggtagggcgg gtagtcaaaa
    10681 tacgcatatg cctttcaagt tgaatatggc tggtgttatt cccccaattt ttgcttccag
    10741 tattattcta tttccatcta ctcttttagg ttggtttggt tcggctgata caaatagtgt
    10801 tttgcacaaa atagctggat tgttacaaca cggtcaattg ctgtatatgg ctttatttgc
    10861 agcgacagtt attttctttt gttattttta tacggctttg gtttttagcc ctaaagaaat
    10921 ggcagagaat ttaaaaaaga gtggtgcttt tgttcctggg attagacctg gtgagcagac
    10981 ctctaggtat ttagaaaaag ttgtattacg tttgacattg tttggagctc tttatattac
    11041 aactatttgt ttaattccag agttcttaac tacggtttta aatgtacctt tttatttggg
    11101 tggcacgtct ttgttgattc tagttgttgt aacgatggat tttagtacac aaataaattc
    11161 gtataggctt actcaacagt atgataagtt aatgactcgt tcagaaatga aatcattttc
    11221 tcggaaatag aattatggcg aaagaagata ctatccaaat gcaaggtgaa attcttgaaa
    11281 ctttacctaa tgcaacattt aaagtaaaac ttgagaatga ccatattgta ttgggtcata
    11341 tttctgggaa gatgcggatg cattacattc gtatttctcc gggagataag gtcacagtag
    11401 agctgacacc ttatgatcta actagggctc gaatcgtttt cagagcaaga taaaccaata
    11461 aaaggaaaat aaaatgcgtg tacaaccatc tgttaagaaa atttgccgaa attgcaagat
    11521 tattcgtcga aatcgtgtag ttcgtgtaat ttgtactgat ctccgtcaca aacagcgtca
    11581 aggttaatgg aatatttctt ttaatgtgat tctgtgatat agtgacacac tttgccctaa
    11641 aaaggaaaaa atatggctcg tattgcaggg gtaaatatcc ctaataacgc acacatcgta
    11701 attggtcttc aggctattta cggtattggt gctactcgtg ctaaattgat ttgtgaggct
    11761 gcaaatattg cgcctgatac taaagcaaaa gatttggacg agactcaatt agatgctttg
    11821 cgtgaccaag ttgccaagta tgaagtagaa ggtgatttgc gtcgtgaggt aactatgagt
    11881 atcaagcgat tgatggacat gggctgctat cgtggcttcc gtcatcgtcg cggcttacca
    11941 tgccgcggtc aacgcactcg tacaaatgcg cgtacccgca aaggtccgcg taaagcgatt
    12001 gctggtaaga aataaatttt aaggaatttt attaatggct aaagcaaaca cagcttcacg
    12061 tgtacgtaaa aaagtacgta aaaccgtgag tgagggtatt gtgcacgttc atgcatcttt
    12121 caacaatacc atcattacaa tcactgaccg tcaaggcaat gcgttgtctt gggctacctc
    12181 tggcggcgct ggttttaaag gttctcgtaa aagtacacca tttgcagcac aagttgcagc
    12241 agaagcagct ggtaaagttg cccaagagta tggcgttaaa aatttagagg ttcgtattaa
    12301 aggtccaggt ccaggtcgtg aatcctctgt acgtgctttg aatgctcttg gtttcaagat
    12361 taccagcatt actgacgtta ccccgttgcc tcataacggt tgccgtccgc ctaaaaaacg
    12421 tcgtatttaa tattggagtg atttgaaaca tggcacgtta tattggccct aaatgtaagt
    12481 tggcacgtcg cgaaggtacg gatttgtttt tgaagagtgc gcgccgctct ttggattcta
    12541 aatgtaaaat tgattccgct cccggtcagc atggtgcaaa aaaaccgcgt ttgtcagact
    12601 atggtttgca gttgcgtgaa aaacaaaaaa tccgccgtat ttatggcgta ttagaacgtc
    12661 agttccgtcg ttatttcgca gaagctgatc gtcgtaaagg ttctaccggc gagttgctgt
    12721 tgcagttgct ggaatctcgt ttggataatg tcgtttatcg tatgggtttc ggttctaccc
    12781 gagctgaagc aagacagctt gtttctcata aggcgatagt tgtgaatgga caagttgtca
    12841 atattccttc tttccaagtg aaagctggtg atgttgtctc agttcgtgaa aaagccaaaa
    12901 aacaggtacg tattcaagaa gcattgggtt tggcaactca aatcggcttg ccgggttggg
    12961 tttctgtaga tgcggataaa cttgagggtg tgttcaaaaa catgccggat cgctcggaat
    13021 tgaccggtga tattaatgaa cagctggtgg tagagttcta ctctaaataa tgctagctca
    13081 gtgagggaca gttaaatgca gaatagcaca accgaatttt tgaaacctcg tcaaattgat
    13141 gtaaatactt tttctgcaac tcgtgcaaaa gtatctatgc agccatttga acgtggtttc
    13201 ggtcatacct taggtaatgc tttgcgccgt atcttactgt catccatgaa tggttttgct
    13261 cctactgaag tagctattgc cggtgtatta cacgaatatt ctactgttga tggtattcag
    13321 gaagatgttg ttgacatttt gctgaatatt aaaggtattg tgtttaaact ccatggtcgt
    13381 agccaagttc aacttgtgtt gaagaaatca ggttcaggtg tcgtatctgc cggtgatatt
    13441 gagttgccgc atgatgtaga aattctgaat cctggtcatg tcatttgtca tttggctgat
    13501 aacggtcaaa ttgagatgga aattaaagta gagcaaggtc gtggttatca atctgtttca
    13561 ggtcgtcagg tagttcgtga tgagaaccgt cagattggtg caatccagtt ggatgcgagc
    13621 ttttcgccca tcagccgtgt tagctttgag gttgaacctg cacgtgtaga gcagcggacg
    13681 gatcttgata agttggtttt ggatatcgaa accgacggtt ctattgatcc tgaggaagct
    13741 gtacgcagtg cggcacgtat tttgattgat cagatgtcta tttttgctga tttgcagggt
    13801 acgcctgtgg aggaggttga agaaaaagca cctcctatcg accctgttct tttgcgtccg
    13861 gtggatgatc tggaattgac agtacgttca gctaattgtt tgaaagctga ggatatttat
    13921 tatattggcg atttgattca acgcactgaa accgagcttc ttaaaacgcc gaatttggga
    13981 cgtaaatctt tgaatgagat taaggaagta ttggcatcta aaggtttgac actgggttct
    14041 aagttggaag catggccacc tgtaggcttg gaaaagcctt aatgaagaat taaaggataa
    14101 ttgatatgcg tcatcgtaat ggcaatcgca aattaaaccg taccagcagt catcgtgctg
    14161 caatgctgcg taatatggcg aattcattat tgactcacga aactattgta acaactctgc
    14221 ctaaggccaa ggaattgcgc cgtgtagtag agccgttgat tacattgggt aaaaagccgt
    14281 cattggcaaa ccgccgtttg gcatttgacc gtactcgcga ccgtgatgtt gtagtaaaac
    14341 tgtttggcga tttgggtcct cgttttactg ctcgtaacgg tggttatgtt cgggtgttga
    14401 aatacggatt ccgtaaaggt gataatgcac ctctggcact ggttgaattg gttgacaaac
    14461 cggctgctga gtaattttag tcatataacg ccatctgccg aaaagcaggt ggcgttattt
    14521 ttgcaatatc tgataggtaa tagggtattg gctatcatgt ttaaaatatt aattgaatag
    14581 ctaaggtttg cgcggtaaac ttacatcatt aaaaaattct atgatggttt atataatgaa
    14641 tgccttcgat ataaagtcga caaagatgga cgtattgtct atatctttgc atacatcaga
    14701 cttgtttgat ttggaagatg tgctggtcaa attgggcaag aagtttcaag agtctggtgt
    14761 tgttccattt gttctggatg ttcaagagtt tgattatccc gagtctttgg atcttgctgc
    14821 attggtttcg ttgttttcaa ggcatggtat gcaaattttg ggtctgaagc attctgatga
    14881 acgttgggct gctgcggcta tgaagtatca tttgctgttt tgtctgtctc attcggaaaa
    14941 tgttaaagaa ctgggtcagg ttgaggtgca gaaaacggag gatggtcaga aagcaaggaa
    15001 aacagtattg attacatccc ctgtccgtac cggtcagcag gtttatgccg aagatggcga
    15061 tttgattgtt acgggggcgg tcagccaggg ggcagaattg attgcggatg gcaatataca
    15121 tatttatgcg ccgatgaggg ggcgtgcttt ggccggcgcc aagggtgata cttctgcccg
    15181 catatttatc cactccatgc aggcagaact ggtttctgtg gcgggtattt accgtaattt
    15241 tgaacaggat ttgccggacc atctgcacaa gcagccggta cagatattgt tgcaggataa
    15301 ccgattggtt atcagtgcaa ttggctcaga gtaattgttt gatattttaa aaaggaaata
    15361 ttgtggcaaa aattattgta gtaacttcag gtaagggcgg tgtcggtaaa acgactacca
    15421 gtgccagtat tgcgacaggt ttggcattac gcggatataa aactgcggta attgattttg
    15481 atgtgggttt gcgtaacctc gacctcatta tgggttgcga gcgtcgtgtc gtttatgacc
    15541 tgatcaatgt cattcagggg gaggcgacgc tcaaccaggc tttgattaaa gataaaaatt
    15601 gtgaaaacct gtttattttg ccggcttccc agactcggga taaagacgct ttgacacgcg
    15661 agggcgtaga aaaagtgatg caggagctgt ccggcaagaa aatgggcttt gagtatatta
    15721 tttgcgactc tcctgccggt attgagcagg gtgcattgat ggcgttgtat tttgctgatg
    15781 aagccattgt aacgaccaat cccgaggttt ccagtgtgcg tgactccgac aggattttgg
    15841 gaattttgca aagcaaatcc cataaggcag agcaaggcgg ttcggttaaa gaacatctgt
    15901 tgattacgcg ttattctccc gaacgtgtgg caaaaggcga aatgctgtct gtacaggata
    15961 tttgcgatat tctgcatatt cctttgctgg gtgtgattcc cgaatcccaa aacgtcttgc
    16021 aggcatccaa ttccggagaa ccggtcatcc atcaggacag cgtggcggct tccgaggcat
    16081 ataaggacgt tattgcccgt cttttgggcg agaaccgtga aatgcgtttc ttggaagctg
    16141 agaaaaaaag cttcttcaaa cgtctgttcg gaggatgagg tatgtcatta atcgaacttt
    16201 tattcggcag aaagcagaaa acggcaaccg ttgcccgcga ccgccttcaa atcatcattg
    16261 cccaagagcg cgcccaagaa ggtcaggctc cggattacct gccgacttta cgtaaagagt
    16321 tgatggaagt cctgtccaaa tatgtggatg tttcattaga caatatccgt atttcccagg
    16381 aaaaacagga tggtatggat gtgcttgagt tgaacattac tttgccggaa cagaaaaagg
    16441 tataggacat gaccttaacc gaattgcggt acatcgtcgc agtcgcccaa gaacgtcatt
    16501 tcggcagggc ggcgcggcgt tgttttgtca gccagcccac tttgtctatt gccattaaga
    16561 aattggaaga agagcttgcc gtctctttgt ttgaccggag cagcaacgat attattacga
    16621 ccgaggcggg ggaacgtatc gttgcacagg cgcgtaaggt gttggaagag gcagagctta
    16681 tcaggcattt ggcaaatgaa gaacaaaacg agctggaggg tgcgttcaaa ctcgggctga
    16741 tttttacggt tgcgccgtac ctgctgccga aactgattgt ctcgttgcgc cgtactgcac
    16801 cgaaaatgcc tttgatgttg gaagagaatt acacgcatac tttgaccgag tcgctcaaac
    16861 gcggggacgt tgacgcgatt atcgttgccg aaccgtttca agagccgggc attgttaccg
    16921 aacccttgta tgacgaaccg tttttcgtga ttgtcccgaa agggcattca tttgaggaac
    16981 tggatgccgt ttcgccacgg atgctgggtg aggagcaggt tttgctgctg acggaaggca
    17041 actgtatgcg ggatcaggta ctctcaagct gttccgaatt ggcggcgaaa caacgcatac
    17101 aggggttgac caatacattg cagggcagct cgatcaatac aatccgccat atggtcgcca
    17161 gcggtttggc aatcagcgtg ttaccggcaa ccgcgctgac cgaaaacgat catatgctgt
    17221 tcagcattat tccgtttgaa ggtacgccgc caagccggcg ggtcgtattg gcgtaccgcc
    17281 gcaattttgt ccgtccgaag gcgttgtcgg cgatgaaggc ggcgattatg cagtcgcagc
    17341 ttcacggggt aagttttatc cgcgactagg cgcaggcatt gttttcaaaa cgccatttcc
    17401 ctgagccgac aacacggtat gccaagatat tgccgtcatc atcgattttg agtatagcat
    17461 cgccacggaa actgccgtcc tgaagatatt cgacttttgc atcactgtga atgttttcat
    17521 cagtgccgat gcaatgccat gtatagtgga ttaacaaaaa ccagtacggc gttgcctcac
    17581 cttgccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
    17641 actataaaag aggccgtctg aaaaacattt ttcagacggc cttgttgatt caatcaaatc
    17701 agtctttcaa cttcgacaac tgagtttgca cttttgccat tttgtcttcc aattccgcca
    17761 aatcggcttt gtctttttcc accaaatgcg ccggggcttt ttcggtgtag ccgggtttgg
    17821 agagtttggc gttgagtttg tccaaggctt tttgcagctt ctcggcttct ttgctcaaac
    17881 gggcggtttc ggcagctttg tcgatttcga ctttcaacat caggcgcgcg ccgttgcaga
    17941 cggcgacggg cgcgtcttcg ctttcgggta gggcggcgac ttgctgtgct tcggtcaggc
    18001 gggtcatcat cggcaggtat ttgaggtagt ccgccaagtc gtctgcgctt tcgacaaaca
    18061 gcggggcttt cacgttgggc tggatgccca tttcgccgcg caggttgcgg actgcgccga
    18121 tcaaatcctg caacacggtc atttgcccga atgccgtctg aacaatctcg ccgccgtcgg
    18181 cttcggggaa gcgggcgagc atgatgctgt cggcggtttt cgcgtcgcac ataggggcga
    18241 cggtttgcca cagttcttcg gtgatgaacg ggataatcgg gtgcagcagg cgcagggcgg
    18301 cttcgagtac gcgcaataag gtatggcgtg tggcgcgttg gcggctggcg cagccggttt
    18361 gaagctgcac tttggcaagt tccaaatacc agtcgcaata gtcgttccac acgaagctgt
    18421 acagggtttc cgccgccaaa tcaaagcggt aggtttcgta ggcttgcgta acctgctcga
    18481 tggtctgatt cagacggcct acaatccaca tatcggggaa ggaatagccg cgcggttcgg
    18541 tggcggttgc gccgtagccg cagtcttggt tttcggtgtt catcaagacg aagttggtgg
    18601 cgttccagat tttgttgcag aagttgcggt agccttcggc gcgtttgaag tcgaagttca
    18661 cgctgcgccc caagctggcg tagctcgcca tggtgaaacg caatgcgtcc gcgcccatac
    18721 tcggaatgcc ttcgggaaag agttttttgg tggcttcttc cactttcggc gcggtttcgg
    18781 gtttgcgcag accggtggta cgtttcacca gcagtttttc caagtcgatg ccgtcgatca
    18841 aatccacagg gtcaatgacg ttgccttcgg atttggacat ttttttgcct tcgtggtcgc
    18901 gcacgatgcc gtggatgtac acgtctttaa acggtacttt gccggtaaag tgggtggtca
    18961 tcataatcat gcgcgccacc cagaagaaga tgatttcgta gccggtgacc aagacgttgg
    19021 acggcaggaa ggctttgagt tcgtcggttt cagacggcca gccgagcgtg gagaacggca
    19081 cgagtgcgga ggagaaccat gtgtccaata cgtcttcttc tcgggtcaag cctgttttgc
    19141 cggcttgttt ttcggcttct tcctgattgc gggcaacgta cacattgcct tcgttgtcgt
    19201 accatgcagg gatttgatgc ccccaccaca gttggcgcga gatgcaccag tcttggatgt
    19261 tgttcatcca ttggttgtag gtgttgaccc agttttcggg gataaagcgc accgcgccgc
    19321 tgtcgacggc ttttttggct ttgtcagcaa ggcttaagcc tttgaattcg ctgtcaggct
    19381 cgccgccgtt tggcgtggcg gacatggcga caaaccattg gctggtcagc atcggctcaa
    19441 tcacggaacc ggtacggtcg cctttcggcg tcatcagcgt gtgcgctttg atttcgacca
    19501 agaaaccttg ttcctgcaaa tcggcaacca tttgtttgcg cgcggcaaag cggtccaagc
    19561 ctgcgtattt ttcaggcagg gcaaagcttg gttgcgcttc gcctttgaag ttgaacactt
    19621 cggcgtttgc caatactttg gcttccaagt cgaacacatt aaccaagcgc gtatcgtggc
    19681 gtttgccgac ttcgtagtcg ttgaagtcgt gcgcaggcgt gattttcacg cagccggtgc
    19741 cgaagtcttt ttcaacgtat tcgtcggcaa tcacgggaat ggtgcggccg gtcagcggca
    19801 ggattaattc tttgccgatt aagtgggtat aacgttcgtc ttcgggattg acggcaacgg
    19861 caacgtcgcc caacagcgtt tcgggacggg tggtggcgac gatgacggct tcggcaggat
    19921 tgtccgccag cggatagcgg atgtgccaca tagagccttg ttcttccacg ctttccactt
    19981 ccaaatcaga cactgcggta cccaataccg gatcccagtt caccaagcgt ttgccgcgat
    20041 aaatcaagcc ctgttcatac aggcgtacga acacttcggt tacggtttcg gcgcgtacat
    20101 cgtccattgt gaaatactcg cgcgtccagt cggcagaaca gcccacacgg cgcatctgct
    20161 gcgtaatcgt gccgccggaa acttctttcc attcccacac tttttccaag aatttttcac
    20221 gacccaagtc atgacgggac acgttttgcg cggcaagctg acgctcgacc acgatttgcg
    20281 tggcgatgcc cgcgtggtcg gtgccgggaa tccaggcggt gttgcagcct ttcatgcggt
    20341 agtagcgggt cagaccgtcc ataatggttt ggttgaaggt atggcccatg tgcagcgtgc
    20401 cggttacgtt gggcggcggc agttggatgg aaaaagacgg tttggtcaaa tccatatcgg
    20461 gctggaaata accttggctt tcccagtttt gatagtgttt ggattcgatt tcggcggggg
    20521 agtatttgtc taacatgatg gaactttgtg aaattaaggt tatttttgat gtgcggatta
    20581 taacgcaaaa aggccgtctg aatcatttca gacggccttt ggcatacggg ttttaaaaat
    20641 ggaacaatac caggctgacg gcaatcaccg ccatacccgt tgtcaggccg taaacggttt
    20701 catggccgtc tgaatagcgt ttggcggccg gcagcagctc gtccaacgcc aaaaacacca
    20761 tcacaccggc tatcacgccg aataccgaac caaacacggc aggcgacaaa aacggctgca
    20821 aaaccaaata gcccaaagcc gcccccaacg gctcggccaa gccggatagc agacacgccc
    20881 acaccgtttt cttacggctg cgggtggcaa aataaaccgg cgcggcgatg gaaatgccct
    20941 ccggaatatt atggatggca atcgccaagg ccaaaggcat cccgaccgcc ggattttcca
    21001 atgtggcaaa aaacgtcgcc aagccttcgg ggaaattgtg cgcagtaatc gcaaacgccg
    21061 ccatcatgcc gactcgcgcg atatggcggc gtttgctttc ttgaaacgac gggtcttgcg
    21121 cgtctaaagt ttcatgcggg ttcggcacca gacggtcaat cagcgcaatg ccgcccatcc
    21181 cggccaaaaa tgccatggtc gccgccgcaa acgcgtggtc tttatcataa atttcagcga
    21241 acgcctcgct ggacttactg aaaatctccg tcagggaaac atacaccatt gcaccgccgg
    21301 caaatgccaa accaaacgac aacacacgcg gattgggcgt tttggaaaac attaccaagc
    21361 cgctgcctaa tacggtaaac aaaccggcag ccaacgtgat ggaaaaggca acggccaaat
    21421 tggacatcga aaaatcgggc atgagaaaac ctgcgctaaa agctgggaca ggttcagact
    21481 aacacttttt aatgcgtatg ataatagtta ttatttattt tattggttgg atacacggat
    21541 tttgaaacaa aaggccgtct gaaaaatagt tttcagacgg cctttacatt tgaaatgcca
    21601 ctaaacctta tcgctttcca gcttaagcct gatagcgcga caggctcaaa tcgtcgctgc
    21661 ggatttcggt gtctttgccg ctcacgatat cggcggttaa ttttgccgaa cccagcgaca
    21721 tggtccagcc taaagtaccg tggccggtat tcagaaacag gttgtcaaag tgggtgcgac
    21781 cgattaacgg cgtgctgtcg ggcgtcatcg gtctgaggcc gctccagaac gatgcttggc
    21841 tcaaatcgcc gccttccggg aacaagtcgt tgacgaccaa agccaaggtt tcgcggcgtt
    21901 tttcgggcag tttgatttcg tagcccgaca attctgccat gccgccgacg cggattctgt
    21961 tgtcaaagcg cgtgatagcg actttgtagc tttcatccaa aacggtggac accggcgcgc
    22021 cgtctgaatt ggtgaccggc agggtcaagg aatagccttt gacgggataa atcggcagat
    22081 cgaggtctaa ctgcgccaac acagtcctac tgaagcagcc gagcgcacag acaacggcat
    22141 ctgcttcaaa ccgccctgtt ttggtttcaa cggctttgat gcgcagcccg ttatggtcga
    22201 tgcggctgat ggtttggttg aaatgaaact gtacgccctt ttcctgacac aatttgtata
    22261 ggttttcggt gaagaggtgg cagtcgccgg tcgcatctgc aggcaggtgc aggccgccgg
    22321 caattttggc ggtaacgcgt gccagcgcag gctcaaattc tgcacattct tcgggtttca
    22381 gacggcggta cggcacgccg tagcgttcca aaacggcaat gtcttgttct gccgcttcaa
    22441 cttctttagc ctgacggaaa atctgcaacg tccctttttt gcgttcctca aaattcatgc
    22501 cggtttgcgc ttcaaaacgg cggaacattt cacggctata ttcggaaatc ctgaccatgc
    22561 gctctttatt ggtttgatag tgcgctgccg tgcagttttg cagcatttgc cacagccatt
    22621 cgatttgata caggctgccg tcggggcgaa acagcaaagg cggatggctt ttaaacagcc
    22681 atttcagtgc tttggtcgga ataccgggtg cagcccaagg cgtggtatag ccgtaagaaa
    22741 gctggcctgc attggcaaaa ctggtttcca tcgccacacc ctcggcgcgg tcgatgaccg
    22801 ttacttcatg tcctgcctct gccagatacc acgcggaaga cacgccggca acacccgcac
    22861 ctaaaacaag cactttcatg tttctccctc cggctttttc aaaacagact taatatgccg
    22921 tgccgtctga atattcggat tcagacggcc tcggatatta atgcggcaat tcgccatttg
    22981 tgattttttg tttgaagtcg cgcgtttcat tgacgatgac tttcgccatc aataaaagtg
    23041 caatcaggtt gggcaatgcc atcaagccgt tgaatgtgtc cgaagccagc cacaccaaat
    23101 caaggctcaa cacggtaccc agcataacgg aagaaacata accgacgcgg tacaaaccgg
    23161 caaatttctc gccgaaaaca tacaccgcgc atttttcgcc gtaatagcac cagccgagaa
    23221 tggttgagta ggcaaagaaa atcaggccga tggtaacaat ccagccgccg atgccgggca
    23281 gcattttttg gaatgtgacg gttgtcagtg ccgcgccgct cacttcaggt ttgacaaact
    23341 cgccgcccgc gccgagcagt cccattacca acacgatgcc ggtaatcgag caaacgacga
    23401 tggtatccaa aaacgtaccg gtcatagaaa ccaaggcctg acggacggga tggtcggttt
    23461 tcgcggctgc ggcggcaata ggcgcagaac ccatacccgc ctcattggag aacacgccgc
    23521 gcgccacgcc gtagcggatg accgtaccga tagcgccgcc cgccactgcc tgcgcgctga
    23581 acgcatcgga gaaaatcagc ttgacggcag gcatcagtgc atcggaatta atcgcgataa
    23641 tggaaagacc gcccaacaca taaaacaccg ccatagcagg cacgatgaaa gaagcggctt
    23701 tggcgatgcc tttaatacca cctaaaacga caacggcagt cagaacggtc aacgtaatgc
    23761 cggtataggc aggttcgata ccgaagctgg tttgcaccgc ctgtgcaacc gagttggact
    23821 gcaccgagct gccgataccg aaggaagcga atgtgccgaa cagcgcaaat gcaacggcca
    23881 tccatttcca gtttttgccc aagccttttt cgatgtaata catcgggccg ccggacattt
    23941 cgcctttgga attgttgacg cggtatttca ccgccaacac gccctcgccg tatttggtgg
    24001 ccataccgaa aatggcggtt atccacatcc aaaataccgc gcccgggccg ccggttacca
    24061 ccgcagtcgc cacgccggcg atgttgcccg taccgatggt ggcggacagc gcggtcatca
    24121 acgccgcaaa atgggaaata tcgccttcgt ggccttcgcc gccttcatac ttctttgacg
    24181 gcacaaacgc ctgtttcagc gcatagccca acatagtgaa ctgcaagcct ttcagcaaaa
    24241 cggtcagcac aatgcccgtg ccgaccagca gcatcaacat aacagatccc cagacccagc
    24301 cgctgacggt ttcgaaaaag gctttgagat tgtctaaaaa caattgcatg gctttctcct
    24361 ttgtctgttt tatttttaaa acaccacttt tgtagtgtcc tataatttca gcacagaata
    24421 ttcaataaga caatatgttc ttttgaaaaa tacttttggt tttttcgccg aaagcaggtc
    24481 ggttcaagct gcggaaattg tttgcaattc tttaaaagca gcggcggagg tcacaatgaa
    24541 atgtccgaat ggggatgtgg cgggcggcag aaatcatcaa tgctgccgac tgccatactt
    24601 ctgaaatcta caaaatgatg catcgatcaa acaatatacc gctttaaaaa aaccgatgcc
    24661 gtctgaaacg ctttcggggc ttcagacggc atcaaaagga tacggtcagc ggatgatgcc
    24721 gcgcgtcgat tgtgcgaaaa agtctcggaa tacggcaagc tcggcttggg tttcggcgcg
    24781 gcggaggatg tctgccttgg cctcttcaaa cggaatgccg cgatggtaga gggttttgta
    24841 cacgtttttg acggcggaaa tctgctctgc ggtaaaaccg ttgcggcgca tgccttcgct
    24901 gttgagcccc gccggttcgg cgcggtagcc cgatgccata aagtagggcg gcacgtcttt
    24961 gtgtacgcct gcggcaaacg cggtcatggc gtagtcgccg atgcggcaga attggaaaac
    25021 cagcgtgtag ccgcccaaaa cgacgtagtc gccgacggta acgtgtccgg caagcgaggc
    25081 gttgttggcg aagatggtgt ggttgccgac cacgcagtcg tgcgcgaggt ggcagtacgc
    25141 cataatccag ttgtcgtcgc cgatacgggt ttcgccgatg ccggttaccg tgcctaaatt
    25201 aaaggtggtg aattcgcgga tggtgttacc gttgccgata atcagcttgg tcggctcgtc
    25261 gcggtatttt ttgtcctgcg gaatttcgcc gaggctggca aattggaaaa tgcggttgtt
    25321 ttcgccgatg ctggtgtggc cgttgatgac ggcgtgcgga ccgatttcgg tattcgcacc
    25381 gatttggacg ttggggccga taacggtgta cgcgccgact ttgacgccgg agtcgagttc
    25441 ggctttgggg tcgatgacgg cggtcgggtg gatgagggtc atgttttgaa tactccgctg
    25501 cttgttaaga caaataatag aaaattacag tttgtcgatt tcatcgcaga ccgcaatcac
    25561 ttgatcgcta aaatggctaa atactgaagc agtatctaca tccatgtaat taagcagttc
    25621 aaaaatagat tggtgaatat ttctagcgta gttggtatct tttattgcac ctttaaaaca
    25681 aatcggctcg tgatgtgcca aacgattacg gaatttgtta atattggaaa gttctcgaaa
    25741 aatccatttt tggttatatt gcacacttgg tgtagatttg ggcttttttg gaaaaacttt
    25801 catcaatact ttccctgtaa catcaaattg ggcatctttc cctcctgcaa acagataccg
    25861 ccaaaaaccg aatcctagtc ctgcaaccaa ttggttgtgg tcaaactttc ccccgctatt
    25921 attttgtatt ttagataatg caaccttgat taattcagca gattctttcg tgccatttct
    25981 taaaaagcaa ccttgatatt ttaatgcggg attcgtctgt ggctgtatgc tgttatataa
    26041 ccagtttctg tctttaaacg cctgctgaag gcaaatgtca attttgtttc ttaaaacaat
    26101 ctcaaatatt gaaacaacat gaaaaagttc tcttgataaa gataaattca ataaatacaa
    26161 ttcagctgct ttgtcttccc caacggcatt tttataccgt tccattctga aatcagaaaa
    26221 aatactacag cattccttat aatccacgct aacacctgat tctttatttt taaattgacg
    26281 tttcaaacta tatttgatac agttaggtaa aactaatccc caccgtgtcc ctgggtttga
    26341 ataaacctaa accggtgggt tttcttttta ttaggtcgtc tgaaactttc agacgacctt
    26401 tttctgaaca ctcaaaccac gcgtttggca cacatgatga tggcttcgac ggcgacttgc
    26461 ccgtccactt tggcaacggc gttgaatttg ccgatgccgc gccggctggt cagcagctcg
    26521 acttcaaaga cgagttggtc gccggggatg acttggcgtt tgaagcgggc ttcgtctatg
    26581 ccggcgaaga agaagaattc gttttctttg cgcccgccct cgctcaaaat cgccaacgtg
    26641 ccgcacgcct gcgccatcgc ttcgatgatg agtacgccgg gcatcacggg caggtcggga
    26701 aagtggcctt ggaactgcgg ttcgtttatg gttacgtttt tgatggcggt cagggttttc
    26761 atcggctcga aggcggtgat gcggtcgagc tggagaaacg gatagcggtg ggggatgagt
    26821 ttttggatgt ctttggcttc gatggggagt tgtacgtcca tgttcgtcgt attccttgaa
    26881 taaagtcggt ttggttattt gctgtcttga ccggcatctg aaagctgctg ctccagtgtt
    26941 ttgaggcgtt tgttcatttc gcttaagcgg tggatgtaaa cggcgttgcg cgcccattct
    27001 ttatgggtgg acatcgggaa gatgccggcg aggtgtttgc cgctttcggt aatgctgtgg
    27061 gtgacggacg tgccgccgcc gatggtggtt ttgtcggcga tttcgatgtg tccgaccgta
    27121 ccgaccccgc cgccgatgat gcagtagctg cctatggtta cgctgccgga gatgccggtt
    27181 ttggcggcga tgacggtgtg cgaaccgatt ttgcagttgt gcccgatttg gacttggttg
    27241 tcgattttgg tgccgttgcc gacggtggtg tcgctcatcg cgccgcggtc gatgttggtg
    27301 ttcgagccga tttctacgtc gtcgcccagc gttaccgcgc cggtttgcgg gattttgaac
    27361 cacgaatcgc cggcaaaggc gagtccgaaa ccgtccgcgc cgatgaccgc gccgctgtgg
    27421 atttcgacgc gtctgccgag tgtgcagccg taataaacga cggcgttggg atgcaggacg
    27481 acttcgtcgc ccagtctgca atcgtgttgg acgacggcgt ttgccaagat gcggcagcct
    27541 tcgccgagta cggtgttcgc gccgatgtag gcgttcgcac cgatttcgca gcttgcgggg
    27601 acggtcgcgc ccggttcgac gacggcggtc ggatggatgc cgccgcgcgc tttgacgacg
    27661 ggtgaaaaca ggcgcgcgac tttggcgaaa tagagatagg ggtcgtcggc gacaatcagg
    27721 ttgcgccctt caaatccgtc tgccgctttg gcggaaacga tgaccgcgcc cgcgctgctg
    27781 tcgtggactt cggctttgta tttcggattg gcaaggaagc tgatgtgttc cgcctgcgcg
    27841 tctgcgagcg ggcgcacggc ggtaacggaa atgtcctcgc cgcgccattc gccgccgagc
    27901 cgcgcggtga tttgggacag ggtgtaggtg gccggaatca tggttttcct gttcggtatg
    27961 ccgtctgaaa gggtcagcgg gcgttcattt ctttaatgac gctgtcggta acgtcgtatt
    28021 gggtgttgac gtaaatcacg ttctgcaaaa tgacatcgta accttcctgt ttggcgattt
    28081 tgacgatgac gcggttggcg ttttgctgga gggaggcaaa ctcttcgttg cggcggaggt
    28141 tgtagtcttc ttcaaactgc gcctgttttt tgcggaacgc tgcgaccagc ccgcgccatt
    28201 tttcttcggc ttgcaccttt tttgcgtttc tgagtttgcc ttcggcaagc tgcctttcca
    28261 aatccagacc ttcgcgttgc agtttttgca attcgtcctg acgagcggaa aattcgctgt
    28321 ccagcgtttt ttgaatcttg cgcgcctgct tggattcgag gtagatgcgc tcggtgttga
    28381 taaagccgat tttttggaag gtgtcggcgt gcgcgcctgc ggtgcagcac aaaccgatca
    28441 gagccgcggc aaacgcgcgg gtcaaacggg tcatggtaaa actccttcga atgttgccgc
    28501 gaaatgccgt ctgaagggct tcagacggca tttgcgggat tagaacgtcg tgccgagttg
    28561 gaattggaag cgttggattt cgtcttccgg ttttttcttc agcgggtagg cgtagctgaa
    28621 tttcatcggg cctaaaggcg agagccaggt aaccgcgccg ccggcggaat agcgcaattc
    28681 gttggtaaag gtggatttat gggtattgcc ggcgccgtaa atgttttgaa ccctgccgcc
    28741 ggtcgcggaa ctgctgttgt cgtcgtaggt tttgccgtcc cacacgctgc ctgcgtcggc
    28801 aaacaggctc aggcggacgg tgcgcgcgtc tttcgcgccg ggcatcggga agagcagctc
    28861 ggcggagacg ttggcttttt tgttgccgcc gtagctgatt ttttcgccgt attcgtcata
    28921 gactttcgga ccaagtgtgc cgctttcgta acctcgtacc gaacccaggc cgccgccgta
    28981 gaagttttca aagaagggga tttctttggt tttgccgtag ccgttggcat agccgatttc
    29041 gccgccaagc atcagtgtga aggttttgct cagggggaag aaccaggttt ggttgtgggt
    29101 ggcggagtag tattgcagtt tgctgccggg cagggcgatt tcggcgttca cgcccgtcag
    29161 gtagccgcgc gtcggccata acgcgctgtc ggttttgttg cgcccccagc cgacggtacc
    29221 tttgtacagc cagcctttga agctgccgtc tgtgccgtcg gttttgccgt attgtttgat
    29281 aaagtcggca tagtgtttgg gcgctttgtt gtaggtgttg acggtcaggt gttctgccac
    29341 caaaccgaaa ttcacgcggt cgtattcggt aacaggcacg ctcatgcgga tgcctgcgcc
    29401 tgccgtggtg gttttatatt gtttgatgct ggtcgatgct ttgcgcgggt cgaaggcttt
    29461 tccgtaaaca tcgtagccta ggctgacccc gtctgccgtg aagtacgggt cggtaaacga
    29521 cagcgagccg ttgagcgtgg ttttgcttcg tgaggcgcgc agggcggccg acttgcccgt
    29581 accgaacagg ttgtcttggg aaacgcctgc ggacatgacc aggccggtat cctgtaccca
    29641 gcccgcgctc aagtcgagcg agccggtgga acgttcggtc aggctcatgt tcaaatcgac
    29701 tttgtcgggt gtgccggcaa gcgggacggc atcaaactgt acgttgtcga agtagcccaa
    29761 aagctcgacg cgctctttgg agcgttgcag cttggaggtg tcgtaaggcg cggattccat
    29821 ttggcgcaat tcgcggcgca cgacttcgtc gcgggttttg ttgttgccgg tgatgtggat
    29881 ttcgttgacg tagattttcc ggcccggttc gatgtgcagg acgaaatcga cggttttggt
    29941 ttcggcgttt ggcagcggct gtacgctgat ttcgctgtat gcgtagcctg ccgagcccat
    30001 gcggttctga atctcaccca aaacggcggt catctgctgg cgttcgtacc atttgccggg
    30061 cttcatggtc agcagttttt ccagttcggc tttggggact tcgttggtgt cgccttcgat
    30121 ggagactttg ccccaacgga aacgtccgcc ttcgtggacg gtgattttga tggtctgctt
    30181 ggttttgtct tcgttggttt ggatgtcggt atcgaggatg cggaaatcga agtagccgtt
    30241 gttctggtag aagtcggtta ctttttccat gtcttgggca aatttctgct cgttgaattg
    30301 gttgcttcgt gtcagccatg tccaaatgcc gccttcggtc agcgacatct gccgcatcag
    30361 tttgcggtcg gaatagactt ggttgccttc aaattcgatg tcggtgattt tggcggattt
    30421 gccctcgtca atcgtgatgt cgatgtcgac gcggttgcgg gcgagtttgg ttactttggg
    30481 cgtgatttgg atattgagtt tgccgcgccc gaggtattct tctttcaggc cggcgactgc
    30541 ctgattgagt gtcgcctgat taaagtattg cgactgcgcc agcccgaacg attcgaggtt
    30601 tttcttaatg gcgtcgtttt gcagcatttt tgcgccggtg atgttgagcg agccgatggt
    30661 ggggcgttcg ataacggtca gcaggagctg cccgtccgca gtttcgacgc gtacgtcgtc
    30721 aaagaaaccg gtggcgtaca ggcttttgat gatggcactg ccgtgtgtgt cgttgtaggt
    30781 gtcgccgact ttgacgggca ggtagttgaa tacggtactc ggctcggtac gctgcaagcc
    30841 ttcgacgcgg atgtcttgga tggtgaagtc ggcaagtgcc aaaggcgata tgcccaagac
    30901 catcagtgcg gaagcaatct gtttcagttt cattgtcagt tccttgtggt gcggaatgcg
    30961 gtttcagacg gcattccgaa acgtaaaatc taaccgagca gccgggtaac gtcgttgaag
    31021 aaggcgaccg ccatcatcag catcatgagg gcaagcccga agcgcaaacc gatgttttgg
    31081 acgcgttcgc ccaaaggttt gccgcgtatc cattcggcag tataaaacac gaggtggccg
    31141 ccgtccaaaa cggggacggg cagcaggttc agcacaccga ggctgatgct gaccagtgcc
    31201 aaaaattcca aataactttg caagccgagt tcggcggact gtccggcaat gtcggcaatg
    31261 gtcagcggcc cggaaatatg gctgacggag gcgttgccgc tgattagttt gccgaaaaat
    31321 ttgagggttg tccacgagtg ggaaacggtt ttttcccagc ccatgccgaa tgcgcggata
    31381 acagacggac ggtagctgcg gcggatttgc gcgtcccacg ccctgtccgg ctgcggacgg
    31441 aggccgacgc gcccgatcag ggtgtggtcg ggctgttcga cagtatcggg gcggatgtcg
    31501 gcggtatggg tttgtccggc gcgttcgtag gtcagggtga tttttttgcc ggggctttgg
    31561 cgggtcaggt ttgcccattc ctgccatgag gcgatgggtt tgccgtcggc ggcagtcagc
    31621 ctgtcgcccg gtttcaggcc tgctttttcg gcggggctgc ctttttccac gccgccggca
    31681 acggttgtga ttttaaaggg catcagtccg atgtagcctt ggttttttgc gattttaccg
    31741 gcttccggcg tgcctgcggc atcgatggtg cggacggttt gcgcgcccga tgccgtctga
    31801 acgccgacgg cgactttgcc ggcttcgagg ttgaggacga tttcggtttg cgcgctgccc
    31861 caatctgcaa cgggtgtgcc gttgacggat tgtattttgt cgccgctttg gaagccggcg
    31921 cgggcggcaa tggtgtcggg ttcgactgtg ccgacatagg ggcgcagttc ggttacgccg
    31981 aaggaaaagc tcagtccgta cagcaaaacc gccagtgcga gattggtcag cgggccggcg
    32041 gcgacgatgg cgatgcgttt ggcggggtgt tgtttgtcaa aagcgtaggg taaatcggct
    32101 tctgatactt cgccttcgcg cgtatcgacc attttgacgt aaccgcccaa cggaatcggg
    32161 gcgaggcacc attcggtgtc gccgcgcttt cgggtgaaaa acggtttgcc gaagccgacg
    32221 gaaaagcgta cgactttgac gccgcacaat ctggcaacga tgtagtgtcc gaactcgtgc
    32281 aggctgacca aaatcaggat ggcgaagata aaagctagaa gggtgtgcaa atggttttcc
    32341 tttgataacg gtgttcagac ggcatcagcg cagtgtgccg ataaatgccc gcgcttgtgc
    32401 gcgtgtccgg gcatcttgcg ccaagagccc ccctatgtcg cctatgccgt ctgaaaagtc
    32461 ttgtgaaaga caatgggcga cggttttggc aatgtcggta aacttaatct gtccgtccaa
    32521 aaaggcggcg acggcggctt cgttggcggc gttcaatacg cagggcgcgg ctccgcctgc
    32581 gttcatggct tcataggcga gcttcaggca ggggaagcgg tcaaagtcgg gcttttggaa
    32641 ggtcagcgcg gacaatgcgt cgaaatccag gtcgccgaca cccgaatcga tgcgctcggg
    32701 caaacccaaa caataagcaa tgggcgtacg catatcggga ttgcccagtt gcgccaacac
    32761 ggagccgtcg cggtagcgca ccatgctgtg tatcacggat tgcggatgga tgacgacttc
    32821 gagcttgtca ggcggacagt tgaacagcca atgcgcttca atcagctcca aacctttgtt
    32881 catcatggtg gcggaatcga cggagatttt gcgccccatg ctccaattgg ggtgtttgac
    32941 cgcttgggcg ggcgtaatgc tgtcgaacgt gtttaaatcg gcggtcagaa acgggccgcc
    33001 ggaagcggtc aggataatcg aagcgatgcc gtgttcgttc agacggccgg cgtaatcgcg
    33061 cggcaaaact tggaaaacgg cgttgtgttc gctgtcgacg ggcagcactg ccgcgccgtt
    33121 tgcacgggcg gtttccataa acaacgcgcc ggaaaccacc agcgtctctt tgttcgccag
    33181 ataaatggtt ttgccttttt gcgccgctgc gagcgcggaa ggcagcccca ctgccccgac
    33241 gatggcgcac atgacaccgc tgacttcgtc ggcagacgca acgtcaacca atgcctgcgc
    33301 gccgtgtaaa acctgcgtcg ccgtgccgtc gcgtttcaac agggcttcaa gccgggcggc
    33361 gtgttccgca tcggcaacga cggcatattc ggggcggaac gtttgacatt gagccgccaa
    33421 tttctcgacc tgcttatgcc ctgccagcgc gaatacgcgg aatttttcgg ggtggcggga
    33481 gacaacgtcc agcgtgcttt cgcctatgct gccggtactg cctaatatgg tcaggacttg
    33541 tggtgtcata atgaggataa ctttataccg gatgccgtct gaagcgtttt cagacggcat
    33601 agaatcaatt taaaaccgac atcatcgccg catagacgct gataacggca atcaggctgt
    33661 cggtacggtc gaacacgccg ccgtgtccgg acagcaggtt gctgctgtct ttaatgcccg
    33721 ccgcgcgctt gagccagctt tccaacaggt cgccgcatac gctgacaacg gtcagcacca
    33781 aaccgattaa cacggtatcg aaccagcctg tatcgaatgc cagccagccg gcacttcgta
    33841 cggcggtcat gtacactgcc acgcaaaccg cgccgccgat tgcaccttcc cagcttttgc
    33901 cggggctgat tgccggtgcg attttgtgtt tgccgaacgc cttgccgctg aaatacgcgc
    33961 aaatatcggc aacccacacc aaacccatca cggcgagcag cggcagggca tcatcgggat
    34021 gcgggcgcag tgatacgagc gcgaaccaaa acggcatgac cagaagccag ccgacggcat
    34081 aaacctgcca accgccgttg agcctccatt tgaatctcaa ccataaaggc ataacggcga
    34141 gccaaaatgc caaaacaaca taccaaacca aattaggcag catccagccg cccgcatagg
    34201 caaccacgcc gaaaaccaag gttgcggcga ggtaatggtt ggttttaatt ttgcacaaac
    34261 cgcccatacg ggcatattcc cacaaggcaa tcagggcaat cagtccgcaa aatgcagccc
    34321 acaaccattg cggcgcgtaa aacagcatgc ccagcatcag cggcagcagc cacatggcgg
    34381 ttattacccg ttgtttcagc atattcagtt cctttgctgt tcgataggca gttgctcgga
    34441 ggtgcgtccg aaccgccgtt cgcgtttttg gaacgaagcg acggcatcgt ccaaagcctt
    34501 gccgtcaaaa tcgggccaca aaatatcggt gaaatacagt tctgcatatg ccatctgcca
    34561 gagcaggaaa ttgctgatgc gcgtttcgcc gccggtgcgg atgaacaaat ccggttccgg
    34621 tgcatcgccc agcatcaagt gtttcgccag cgtgtcttct gtaatctcgg atacgccttc
    34681 ggcaatcagt ttgtttgccg cctgcaaaat atcccagcgg ccgccgtaat cggcggcaat
    34741 gctcagggtc aggccggtat tgtttgccgt caacgcctcc gcttcttcga tgccttgcag
    34801 aatctgccgg ttgaagcgtt cgcggctgcc cagtattttc aggcgcatat tgttttcgtg
    34861 cagacggcgt acctgttttt gcaaagcctg taaaaacagc cccatcagga acgaaacttc
    34921 gtcttcgggg cggcgccagt tttcggttga aaaggcaaac acggtcagat attgcacacc
    34981 cagtttggcg caatgcttca ccatattttc caatgcgtcc aaaccgcgtt tgtgtcccat
    35041 tatgcgcggg aggaaacgtt ttttcgccca acggccgttg ccgtccataa tcacggcgat
    35101 atgcttggga atggcggtgt gttccaaaac ggcctgcgtg ctgcttttca tgtctgcctt
    35161 tcgcggttcg gcattcaaat gccgtctgaa cgccgaaccg tgcaggttaa attgccatca
    35221 aatcttcttc tttggcagtc aggagtttgt cggcttcggt aatgtatttg tcggtcagtt
    35281 tttgaaccgc ttcttcgccg cgacgtgcct cgtcttcgga aatttctttg tctttgagga
    35341 gttttttgat gtggtcgttg gcatcgcggc gcacgttgcg gatagagacg cggccttctt
    35401 ccgcttcgcc gcgtacgact ttaatcaggt ctttgcggcg ttcctcggtc agcatgggca
    35461 tcggcacgcg gatcaggtcg ccgacagctg ccgggttcag ccccaagttt gaatcgcgga
    35521 tggctttctc gactttggcc gccatattgc cctcaaacgg tttcacgccg atggtgcgcg
    35581 cgtccagaag cgttacgttg gcaacttggc tgacggggac catgctgccc cagtattcga
    35641 cttccacttg gtcgagcagg ccggtatgcg cgcggccggt acgcactttc gccagatttt
    35701 ctttcagtac ttcgaccgaa cgctgcattt tgccttcggc tgttttttga atatcgttga
    35761 tcatattgtt ctttcggtgg gataaggtgg gcgggagacc gtctgaacgc gtttcaaacc
    35821 tttcagacgg catgaaaact gttaactgcg aatagtaccg ttattcgggc atgacgacaa
    35881 ggcaggcgga ttggggatgc cgtctgaagc ggcaggcgtt tcagacggca tcgtgtccga
    35941 ccgtcagccg tgttcccgtg tttcaagcag gctttggcgc aggtgttggc gttcgtgggc
    36001 atccagccat ttgcggcggg tgcgttgcag caggatgacg agggcggaaa tttcctgacg
    36061 catattggtg ctgagccaga ggaagccctg ccattggtag tggaggtgtt cggcgagggc
    36121 ttccagttcg gggttgatgg cggtgtcgat gcggatgcgg cgggcgtgtc tgccgttgat
    36181 aagggcgacg gtttgttgca ggtcggtttg gagcagtgtg aagtggcggt caagcagccg
    36241 gatttcgctg ccgttgagtt tgggagattg cagcttggcg gcagtggtca ggagcagctc
    36301 ggtggtgttg acaattttac ggtgggcgtg ctgcatggct tccatcatgg cggggctgat
    36361 gcggctttcg cccgatgtgg cggcgaggtg gctgcggctt ttgaccatgc gtgcgttgat
    36421 ttggcgcatt ttaaccatat tctgctccaa acgttcgcgc gtcatacgcc tgccgttgct
    36481 gatttcggca atcattttgc tgcagtcggt caggttgtcg gcaagcatga aacgccacat
    36541 cagtgtggat ttcagcggca gcagtttggc ggcggcgatg gcgatggccg cgccgatgag
    36601 gacgttcatc gcgcgcatca ggccgctgtc gaaccattcg ctgccgttgt cgccgatgag
    36661 catgcacatc gtcagccccg ccagcatagg gacgtagccg tttttgccga ccgccgccca
    36721 gccggccagt gcgcttgccg tgccgacggt gaggtagaag aggaggttgc cgtggaaata
    36781 atgctggttc agccataaaa cgcccaaacc cgcgcccagc ccgatgaccg tgccgagcat
    36841 acgttccacc gccttggagt aaatcgcccc ttgaaactgg agcatgccga ggacgacgaa
    36901 gacggtcatg cctatccatt cgccgtgttg gaggtggagc agccgggcga gtgcggtggc
    36961 gaacaggacg gccccgccga gccgcacggc gtgtatgagg cggcggtggc ggtagcgttc
    37021 gtaggagttg agccagcggc tgacgaggcg gttgcgttgc gaggtgttca tatcggttgc
    37081 cgtctgaagc ggaaatgtga aaaagcacag gcttcccgag gaagggaggg tctgtgcttg
    37141 gtattggtgc cggagaaggg aatcgaaccc ccgaccttcg cgttacgaat gcgctgctct
    37201 accgactgag ctacaccggc ttttttcgtt atgatatata tgaacggttg tttctgcaac
    37261 ttttcgggct gctggcaaag ttttgcacgg tataatgcgg tttgcttcgt gaggagagtc
    37321 ggtgatgtat gtgaacgaga aatatcccta tgcggctttg tttgcggggc tggtgttttt
    37381 gacgctgccg ttcgcactgt cggtgcatga tgccttggca tttgcgttcg gacgggcggg
    37441 gctgctggtg tcggtgtcgg acggcgaatt cggctggcgt ggcggttggg acggcactgt
    37501 ttggtttgtg ttcggtgtgt ttgcgttttt gaatgtggtt gtgtcggcgg gcctgacgaa
    37561 actggcgtac aaaaagatga tgcggcggca ttcgcgttac gcgctgtttc tgtcgggcgt
    37621 ggcggcttgc gcggctgctg cggtcgcgtg gattttcgag ctgttgttgg gcagtgccgc
    37681 cttgggcggg ctgcgcgggg aggcggtgtc ggaatatgcg tttgccgtgt ggctggtgtc
    37741 tatgctgacg ctgcccaaac gcctgacgcg cgcgccggtg cagccggtgg tgtttcacag
    37801 gaaaaaatag gttggaacgg gaaatgccgt ctgaaccccg acacgcggtt tcagatggca
    37861 tgtttttccg ctaacattac gcctgaatat ggacaggaag cagatatgga acgcaaagaa
    37921 cgcctgcgtg caggcattgc cgcgatgggg ctggatattt cggaaacggc gcaggacagg
    37981 cttttggcct atgtggattt gttgaaaaag tggaacaaaa cctacaatct gaccgccctg
    38041 cgcgacgagg aaaaaatgat tgtccatcat cttttggaca gcctgacgct gctgccccat
    38101 atcgagggtg tgcaaacgat gctggatgtc ggttcgggcg gcggtcagcc cggcattccg
    38161 gcggcggtgt gccgtccgga tgtgcagata acccttttgg atgcgaatac gaagaaaacg
    38221 gcttttttac agcaggcggt tatcgagttg gggttggaca atgtgcgcgt ggtatccgga
    38281 cgcgtggagg cggtttcgga cgtgcgtgcc gatgtggtta ccagccgtgc gtttgcagaa
    38341 ctggcggatt ttgtgtcgtg gacggtgcat ctgttgaaag acggcggcta ctgggcggcg
    38401 atgaagggcg tgtatccgca ggaagaaatc ggccgcctgc cgcaggatgt gtgcgttgaa
    38461 aaagtccaaa ggctcgacgt gccgggcttg gatgcggaac gccatatcgt catcctgagc
    38521 aagcgttgag cgcacttcag acggcatgaa tacctttttt gtgcggataa aggtaaaatt
    38581 ccgcactgtt tttctttttt caacatcaga cgggacacgg gcgggacatg agtgcgaaca
    38641 tccttgccat cgccaatcag aagggcggtg tgggcaaaac gacgacgacg gtaaatttgg
    38701 cggcttcgct ggcatcgcgc ggcaaacgcg tgctggtggt tgatttggat ccgcagggca
    38761 atgcgacgac gggcagcggt atcgacaagg cgggtttgca gtccggcgtt tatcaggtct
    38821 tattgggcga tgcggacgtg aaatcggcga cggtacgcag caaagagggc ggatacgctg
    38881 tgttgggtgc gaaccgcgcg ctggccggtg cggaaatcga gctggtgcag gaaatcgccc
    38941 gggaagtgcg tttgaaaaac gcactcaagg cagtggcgga agattacgac tttatcctga
    39001 tcgactgtcc gccttcgctg acgctgttga cgcttaacgg cttggtggcg gcgggcggcg
    39061 tgattgtgcc gatgttgtgc gaatattacg cgctggaagg gatttccgat ttgattgcaa
    39121 ccgtgcgcaa aatccgtcag gcggtcaatc ccgatttgga catcacgggc atcgtgcgca
    39181 cgatgtacga cagccgcagc aggctggttg ccgaagtcag cgaacagttg cgcagccatt
    39241 tcggggattt gctttttgaa accgccatcc cgcgcaatat ccgtcttgcg gaagccccga
    39301 gccacggtat gccggttatg gcttacgacg cgcaggcaaa gggtaccaag gcgtatcttg
    39361 ccttggcgga cgagctgatg gcgagggtgt cggggaaata ggtcaatcca aatcgggctg
    39421 cccgtgcctt tatgctgttt ggccgagtgc gttaatatgg cggattaaaa taaaaatact
    39481 tatatcgtc
//
LOCUS       DAWHXS010000005        38498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWHXS010000005 DAWHXS010000000
VERSION     DAWHXS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.005"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..517
                     /locus_tag="WIE22_000189"
     CDS             <1..517
                     /locus_tag="WIE22_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ4126340.1"
                     /translation="LHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHTHTHFDGKAQV
                     AEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRESEETKFKSRCFGGRSA
                     VRKALYMATVAATRFEPLIRDFYQRLLSKGKPYKVAVTACMRKLLTISNTRMRDYFAE
                     NDTAENGIRTA"
     gene            565..1638
                     /locus_tag="WIE22_000190"
     CDS             565..1638
                     /locus_tag="WIE22_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ4126341.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLPAQTAGFTSLAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRTFLMNVNPIFFITIPAILTAV
                     VFTLYLFTFVPIFRANAFTGDPE"
     gene            complement(1684..3873)
                     /locus_tag="WIE22_000191"
     CDS             complement(1684..3873)
                     /locus_tag="WIE22_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ4126342.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFSDEPLLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVGNRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            4012..4794
                     /locus_tag="WIE22_000192"
     CDS             4012..4794
                     /locus_tag="WIE22_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ4126343.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(5112..7046)
                     /locus_tag="WIE22_000193"
     CDS             complement(5112..7046)
                     /locus_tag="WIE22_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ4126344.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(7112..8290)
                     /locus_tag="WIE22_000194"
     CDS             complement(7112..8290)
                     /locus_tag="WIE22_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ4126345.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATAKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8516..8905
                     /locus_tag="WIE22_000195"
     CDS             8516..8905
                     /locus_tag="WIE22_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ4126346.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVK
                     STLLMNAQNLERYYRQKGTFDNYDQTKLKQNKYFKITLSKVNPDHFTLQAVPDPTTNE
                     GETCIVTLNDGGTLSAAGNGQSCPDFD"
     gene            9189..10235
                     /gene="adhP"
                     /locus_tag="WIE22_000196"
     CDS             9189..10235
                     /gene="adhP"
                     /locus_tag="WIE22_000196"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ4126347.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPESLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDEKLAFAKESGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECSCGHHH"
     gene            complement(10400..13885)
                     /gene="smc"
                     /locus_tag="WIE22_000197"
     CDS             complement(10400..13885)
                     /gene="smc"
                     /locus_tag="WIE22_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ4126348.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TTEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVKLEENESQVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATGHAAAPQLWQHITAPAEWQHALSVILAERLHARDVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLSPELSSAEAAFKQAE
                     ATVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIAILQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLDELT
                     EAVQTLDEEYIAVQEKLAQIQAQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNTSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            14134..14856
                     /gene="lpxH"
                     /locus_tag="WIE22_000198"
     CDS             14134..14856
                     /gene="lpxH"
                     /locus_tag="WIE22_000198"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ4126349.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            15125..16711
                     /locus_tag="WIE22_000199"
     CDS             15125..16711
                     /locus_tag="WIE22_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ4126350.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16880..17083)
                     /locus_tag="WIE22_000200"
     CDS             complement(16880..17083)
                     /locus_tag="WIE22_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ4126351.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            17199..17696
                     /locus_tag="WIE22_000201"
     CDS             17199..17696
                     /locus_tag="WIE22_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ4126352.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNWETFA
                     HIGQA"
     gene            17778..18971
                     /locus_tag="WIE22_000202"
     CDS             17778..18971
                     /locus_tag="WIE22_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ4126353.1"
                     /translation="MFFKHIEAALADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKHLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSRAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAIRAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVALLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(19375..20310)
                     /gene="hemC"
                     /locus_tag="WIE22_000203"
     CDS             complement(19375..20310)
                     /gene="hemC"
                     /locus_tag="WIE22_000203"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ4126354.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            20429..20944
                     /gene="ybeY"
                     /locus_tag="WIE22_000204"
     CDS             20429..20944
                     /gene="ybeY"
                     /locus_tag="WIE22_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ4126355.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            20946..21770
                     /locus_tag="WIE22_000205"
     CDS             20946..21770
                     /locus_tag="WIE22_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ4126356.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(21899..23278)
                     /gene="nhaC"
                     /locus_tag="WIE22_000206"
     CDS             complement(21899..23278)
                     /gene="nhaC"
                     /locus_tag="WIE22_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ4126357.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(23569..24791)
                     /locus_tag="WIE22_000207"
                     /pseudo
     CDS             complement(23569..24791)
                     /locus_tag="WIE22_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            25381..25794
                     /locus_tag="WIE22_000208"
     CDS             25381..25794
                     /locus_tag="WIE22_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ4126358.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            25840..26469
                     /gene="nth"
                     /locus_tag="WIE22_000209"
     CDS             25840..26469
                     /gene="nth"
                     /locus_tag="WIE22_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ4126359.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            26607..28106
                     /locus_tag="WIE22_000210"
     CDS             26607..28106
                     /locus_tag="WIE22_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ4126360.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAENDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLRAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            28308..29828
                     /locus_tag="WIE22_000211"
     CDS             28308..29828
                     /locus_tag="WIE22_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ4126361.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            29885..30970
                     /gene="nagZ"
                     /locus_tag="WIE22_000212"
     CDS             29885..30970
                     /gene="nagZ"
                     /locus_tag="WIE22_000212"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ4126362.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHSNPEAVTRLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLIPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAALASPQDTAGGVKVGEAF"
     gene            31051..31127
                     /locus_tag="WIE22_000213"
     tRNA            31051..31127
                     /locus_tag="WIE22_000213"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31085..31087,aa:Pro,seq:cgg)
     gene            complement(31462..32100)
                     /locus_tag="WIE22_000214"
     CDS             complement(31462..32100)
                     /locus_tag="WIE22_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ4126363.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(32097..32468)
                     /locus_tag="WIE22_000215"
     CDS             complement(32097..32468)
                     /locus_tag="WIE22_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ4126364.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGVTVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(32718..33074)
                     /locus_tag="WIE22_000216"
     CDS             complement(32718..33074)
                     /locus_tag="WIE22_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126365.1"
                     /translation="MNTTSSMIQEEGIIAESIDVINKFVQFLLKLYDDFGIDGMHDLV
                     DPDLDTLESIVKNLQQEVDKLPISPNDFSLENKKISLAQGLLYAQSMITNVRNKDTEE
                     CSRNRSMLKNNQSSLY"
     gene            complement(33183..33605)
                     /gene="queD"
                     /locus_tag="WIE22_000217"
     CDS             complement(33183..33605)
                     /gene="queD"
                     /locus_tag="WIE22_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ4126366.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(33612..34130)
                     /locus_tag="WIE22_000218"
     CDS             complement(33612..34130)
                     /locus_tag="WIE22_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ4126367.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(34158..34817)
                     /gene="queC"
                     /locus_tag="WIE22_000219"
     CDS             complement(34158..34817)
                     /gene="queC"
                     /locus_tag="WIE22_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ4126368.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            34922..35347
                     /locus_tag="WIE22_000220"
     CDS             34922..35347
                     /locus_tag="WIE22_000220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126369.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            35362..36588
                     /locus_tag="WIE22_000221"
     CDS             35362..36588
                     /locus_tag="WIE22_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ4126370.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(36609..36890)
                     /locus_tag="WIE22_000222"
     CDS             complement(36609..36890)
                     /locus_tag="WIE22_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4126371.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(36969..37103)
                     /locus_tag="WIE22_000223"
                     /pseudo
     CDS             complement(36969..37103)
                     /locus_tag="WIE22_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(37102..37560)
                     /locus_tag="WIE22_000224"
     CDS             complement(37102..37560)
                     /locus_tag="WIE22_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126372.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(38124..38327)
                     /locus_tag="WIE22_000225"
     CDS             complement(38124..38327)
                     /locus_tag="WIE22_000225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126373.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT         8931 a        10687 c         9781 g         9099 t
ORIGIN      
        1 tctgcatcag gttcatgaaa cgcaagtcgg aagcgtcaaa caactgattg cccattttga
       61 ccggctgatt gacgaattgg acaaacaaat cgacgaccac acccacacgc attttgacgg
      121 caaagcccaa gtggcagaac aaatcaaagg catcggttcg ataacgacgg ctacgctgat
      181 ggcgatgctg cccgaattgg ggcggctgtc gcacaaacgg atagcgagtt tggccggcat
      241 tgccccgcac ccgagggaga gcgaggaaac caaattcaaa agccgctgct ttggcggaag
      301 gtctgcggtg cgtaaggcac tgtatatggc taccgtggca gcgacacgtt ttgaaccgct
      361 tattcgggat ttctaccaac gcctgctgtc caagggtaag ccgtataagg ttgccgttac
      421 ggcatgtatg cgcaaactgc tgacgatatc gaatacccgg atgcgtgatt attttgccga
      481 aaacgatacc gccgaaaacg gtatccggac ggcttgattt gagttttggt atttttgccc
      541 gacggggtga aaaatacagt tgctatgcgg ccgtttttcg tcggcgcggc ggtgcttgcc
      601 atactcggtg cgctggtgtt tttcatcaac cccggtgcca tcgtcctgca ccgccaaatt
      661 ttcttggaac ttatgctgcc ggcggcatac ggcggttttt tgactgcggc tttgttggac
      721 tggacgggtt tttcgggtaa cctgaaacct gtcgcgactt tgatggcggc attattgctc
      781 gccgcatccg ctatactgcc cttttcgccg caaactgcct cgtttttcgt cgccgcctat
      841 tggctggtgt tgctgctgtt ctgcgcccgg ctgatttggc tagaccgaaa caccgacaac
      901 ttcgccctgc taatgttact tgccgcgttc actgtttttc agacggcata tgccgtcagc
      961 ggcgatttga acctgttgcg cgcgcaagtg catctaaata tggcggcggt gatgttcgta
     1021 tccgtgcgcg tcagtattct tttgggcgcg gaagccctga aagaatgccg tctgaaagac
     1081 ccagtattca tccccaatgt cgtctataaa aacatcgcca ttaccttcct gctcctgcac
     1141 gccgccgccg aactttggct gcctgcgcaa accgccggtt ttacctcgct cgccgtcggc
     1201 tttatcctgc ttgccaagct gcgtgagctt caccatcacg aactcctgcg caaacactac
     1261 gtccgcactt attacctgct ccaactcttt gccgccgcag gctatttgtg gacaggcgcg
     1321 gcgaaattac aaaacctgcc cgcctccgcg cccctgcacc tgattaccct cggtggcatg
     1381 atgggcagcg tgatgatggt gtggctgacc gccggactgt ggcacagcgg ctttaccaaa
     1441 ctcgactacc ccaaactctg ccgcattgcc gtccccatcc tcttcgccgc cgccgtctcg
     1501 cgcactttct tgatgaacgt gaacccgata ttcttcatca ccatccccgc gattctgacc
     1561 gccgtcgtgt tcacgcttta tcttttcacg tttgttccga tttttcgggc gaatgcgttt
     1621 acgggcgacc cggaataaaa atgccgtctg aaacggtttc agacggcatc gtattgccga
     1681 taatcaagcc tcctgcggat cgacatccac cgaccatctg attttgccgt cgcggttttg
     1741 ctgcaacacc tgcacccaca aactcacggc acggtgcaaa tcctgtcggg atgtcgattc
     1801 gaggaagatt tgcgcgcgtt cgcgttcggc gaggcgcacc atcagcatcg gggccgcgcc
     1861 gaactgggaa acgctttcgg gcaacagcgg ggcgagggtt tctttggcgg cattgagaaa
     1921 ttccatcgca tcggcaacgc gcggcgcatc ggcgcggacg gcggtctgga aaccgaaggg
     1981 cggcatggcg aacatttgcc gctcgttcaa ttcgttttcg gcaaacacgg catagtcttg
     2041 cgctttgacg gcggcgaaga caggatgttc gggcagttgg gtctgtatca acaccttgcc
     2101 gggtttgtcg gcgcgccccg ccctgcctga cacttgcatc agctcggcaa acagcctttc
     2161 cggcgcgcga aagtccgcgc tgtacaggct gccgtcggcg ttcaatacga taacgaggtt
     2221 gagccgcgcg aaatcatggc ctttggcaag catctgcgtg ccgaccaaaa tgtcgatttt
     2281 gttgtcggcg atgcggcggt acaaatccgc ccagtcattt ttgtgcgcgg tgctgtccct
     2341 gtcaacacgg acgacggctg ccttgggcag gaaggtgcgc agggtttctt cgacgcgctg
     2401 cgtgccgtgg ccgacggcgg tcaggtcttg gttgccgcag tcggggcatt tgtacgggat
     2461 gggttcgcgg tggtcgcagt ggtgacagcg cagttggcgg gcgcgttggt gcagtaccat
     2521 tttggcggag cagtttgggc agccgaaggt atggccgcag tcgccgcaaa acagcgcggg
     2581 tgcgaagccg cgccggttga ggtacaccag cgacataccg cccgcttcaa aattctgttt
     2641 caaaagctgc aaggcttgcg gcgagaaacc gttgtcgagt ttcagacggc ctacgttgag
     2701 aatgtccact tgcggcagtt gcgcggcggt atgggcgcgt tcggtcagtt gcagcaggcg
     2761 gtacgcgccg ctttgcgcct tgtgccagct ttccaagctg ggcgtggcac tgcccaatat
     2821 gatggggcag ccgccctgct tcgcccgcca caccgccaaa tcgcgggcgt ggtagcgcaa
     2881 ttcgttgtct tgtttgaacg agccgtcgtg ttcctcatcg actacaatca gcccgacatc
     2941 atccatcggc gtaaacaccg ccagccgcgt gccgatgacc aatttcgcct gccccaacat
     3001 cgcgcgcaaa taatcctgcg tgcgcctgcc tgccgccatc tgactgtgca acacggcggt
     3061 cggcacgtcg gcaaaacgat ttcccacccg cttcaaaagc tgcggcgtga ggttgatttc
     3121 gggcaacaga aacaacacct gccgcccctg agccaacact ttcgccatcg catcgaaata
     3181 cacttcggtc ttgccgctgc cggtgatgcc gtacagcaga aacggctgga agcgtcccaa
     3241 tgccgtctga atggcatcgg aagccttctg ctggtcagca tttaatacaa attcagagtg
     3301 cgaagcctgc ccattacacg accttaaaac gggcttcgcc gcttccgtcg tttcaatcca
     3361 accctgctcc gcccaatctt cgattaactt cgccgcctgc gcgtttacct gcttcaacgc
     3421 tgccatcgtc atttcgcccg acagcagcgc gtcccacaaa gccgcttttt tgttgaaccg
     3481 cgccggcggc ggcgtttgcg ccctgcccgc ttcgttcaac gcgtaaaaca agggcggctg
     3541 cggcatttcc accgcgcgcg tttccttcaa accttgcggc aatgcggcaa atactgcctg
     3601 cccggtcgga tagtggtaat aacgcgacgt aaacgagagc aaatcacgcc agctttcggg
     3661 cagcagcggc tcgtccgaaa aggccgtccg aacgctcaaa atccgcgccg tatccatatc
     3721 cggcgcaata tccgtttccc acaccatccc gaccacggtt ttattgcgga aaggcacaag
     3781 cacccgcgtt cccggaggaa gcggatcgga atgggaataa gtcaaaaggc cgtctgaaag
     3841 cggcacgttt acggcgatgc ggtggtagat catacaggca ggcaaatcat ttttttgcgt
     3901 attttaacag gcggaagcta ttttcagacg ataaattgag cctaggcgca atgcaggcgc
     3961 aacagagatt tgatacaatt ccgcttttcc aacacgcgca atcatcttta tatgaaaacc
     4021 aagttaatca aaatcttgac cccctttacc gtcctcccac tgctggcttg cgggcaaacg
     4081 cccgtttcca atgccaacgc cgaacccgcc gtcaaagccg agtccgccgg caaatccgtt
     4141 gccgcctctt tgaaagcgcg tttggaaaaa acctattccg cccaagattt gaaagtgttg
     4201 agcgtcagcg aaacaccggt caaaggcatt tacgaagtcg tcgtcagcgg caggcagatt
     4261 atctacaccg atgccgaagg cggctatatg ttcgtcggcg aactcatcaa catcgacacg
     4321 cgcaaaaacc tgaccgaaga acgcgccgcc gatttgaaca aaatcgactt cgcctccctg
     4381 cctttggaca aagccatcaa agaagtgcgc ggcaacggca agctgaaagt cgccgtcttt
     4441 tccgaccccg attgtccgtt ctgcaaacgc ttggaacacg agtttgaaaa aatgaccgac
     4501 gtgacggttt acagctttat gatgcccatt gccggcctgc accccgatgc cgcgcgcaag
     4561 gcgcaaatct tatggtgtca gcccgaccgc gccaaagcgt ggacggattg gatgcgtaaa
     4621 ggcaaattcc cggtcggcgg cagcatctgc gacaatcccg tcgcggaaac cacttccttg
     4681 ggcgaacaat tcggcttcaa cggcacgccg accctcgtct tccccaacgg gcgcagccaa
     4741 agcggctaca gcccgatgcc ccaactggag gaaatcatcc gcaaaaatca ataatccatc
     4801 cgcaaaacaa aaggcttgga aaattccaag ccttttttgc gggaaatgcc gcctgaaaac
     4861 caatccgcac tgaaagttat agtggattaa atttatagtg gattaaattt aaaccagtac
     4921 agcaagccaa caaacacatc ctaacccatt gaatgattga aaataataaa ttgaacaata
     4981 cgaaacacac cgccgtcatt cccacgcaag tgggaatttg gaacgtagaa tctaagaaac
     5041 cgtttttgtc caataagttt ctgctccaaa atgaccggtt ctgcagcttc aggcggcatc
     5101 tttgtccaac ctcaatcctg tgccaatgcg tctatcggat tgagtttggc tgctttattg
     5161 gcaggcataa agccgaacgc gatgccgatt ccggtcgagc aggcgaccgc cccgataacg
     5221 gatgccgccg aaatgtccat cgggaagtcg gttacaaaat gattgaacac gaggctgacg
     5281 gcggcggaca aacccacgcc gaccaaaccg ccgatgacgc agattaacac cgcctcaatc
     5341 aaaaactgct gcaaaatatt gccgcgccgc gcgccgattg ccatccgtat gccgatttct
     5401 ttggtgcgct cggtaacgga caccagcatg atgttcatca cgccgatgcc gccgactacc
     5461 aatgaaatca gggcgatgga ggaaatcagc agcttcatcg taccggtggt gctttcgact
     5521 atctgcctga tgctgtcgct gttgttcatg aagaaatctt ccgtgccgtg ccgcgctttg
     5581 agcagatcgg tcagcccttt ttcggcaacc tgggtattgg cattgtcttt gattttgacg
     5641 gtgatggagt tggtgtggct ctcgcctgtg atttggtgca tcaccgtcgt atagggcgac
     5701 caaagcatca gcacgtcgga attgccgaaa gcgttttcgt cttttttcat cacgccgatg
     5761 acggtcaagg ggcgtttcct gaacaaaatg gttttaccca acggatccga gtccgcaaag
     5821 agtttgtctt tgacattttg gtcgatgacg acgacctgcg cgtcttcttt cacatcgttt
     5881 tcgtcaaaca gccgccccgt ttccagcttc agtccgcgca cgtcgaaata ttgttcgccc
     5941 acgccgtaca aagaagcggt caggtcggta ttgcggtaag tcagcgtgcc gccgctcgaa
     6001 gtcatgggcg tggcggaagc aacgtagctt tgtttggcga tgatttttgc gtcgtctatg
     6061 gtcagggttt taatcctgcc gctgcgcctg tcgccgaagc cgcgccccgg gaagatgctg
     6121 atggtgttcg tccctatcga actgatgtct tcaaggattt ttttctgcga accgttgccc
     6181 aatgcgacga cggaaaccac cgacgcgatg ccgatgatga tgccgagcat cgtcagaagc
     6241 gaacgcattt tgtgcgccaa tactgcttgc accgacattc tgaaggcttc gacaaactgg
     6301 tcgtaataaa acgaccacga ggctttttcc cgaatcctcc cgacattgct tgcggggatt
     6361 tcgggatttt tcgaggtgtc ggaaatgatt tcgccgtccc ggatttcgat gacgcggttg
     6421 gcattggcgg cgatgccggg gtcgtgcgtg accataatga cagtatgccc ggcttcgtgc
     6481 agcttgtgga tgatttccat cacgtttttg ccgctggcgg tatcgagtgc gccggtcggc
     6541 tcgtcggcga agatgatttc tccgccgttc atcagggcgc gggcgatgga gacgcgctgc
     6601 tgctgtccgc ccgagagttc gccgggcttg ttgccctctt tgcttgccaa acccaaatcc
     6661 tgcaacaact tctccgcacg cgcggaacgc tctttgccgc ccgcgcccat atagacggct
     6721 ggcagcgcga cgttgtccct tgcggtcagc gagcttaaga ggttgtagcg ttggaagatg
     6781 aaaccgaagc gttcgcggcg caatgccgcc agctcgtcag gctgcatttt ggcagtttcg
     6841 atgtcgtcga ttcggtacga accggaaccg gcggtatcca aacagccgag tatgttcatc
     6901 agcgtggact tgcccgaacc ggactgcccg atgatggcga caaaatcgcc cttctctatc
     6961 gacaggctga tgtctttcaa aatatggacg cggttctcgc cgctgccgaa atagcggttg
     7021 atgtttttac attcgatcaa gctcataata ttccgtaaat caataacaaa tgccgtctga
     7081 aaccgtttcc gtgttcagac ggcatattcg tttatcggcg cggcgggccg cctagggcgc
     7141 gttcgccgct ttcctgctgc tcggcggcgg ttatttcgga gatgaccact ttgtccccct
     7201 ctttcaaccc gctttttact tcggtattca tactgtctct cataccggtc cggatttcgc
     7261 gttccgccgc cttgccgtcc gcacccaaca cgcgcacaaa cgccttgccg ccgcgatttt
     7321 tcacggtcag cgacggaata atcagcacat ttttcacgcc gtcgatttca accgtattct
     7381 gcgtcgtcat ccccgtggcg agtttgccgt ccggattcgg cacaaacgaa cgggcataat
     7441 agtagaccgc attggaagcc gtatccgtac tgctgttgta accgcccgac gacatcgtgg
     7501 tcagcccggg gtcgacgctg tcgagcttcg ccttaatcgg cgtatccggt tcggacaaaa
     7561 tcgtaaacga aatatcctgc cccgccttca ccttggtaat atcgccctcg gcaatctgca
     7621 ttttgttcaa catcatatcc agattcgcca attggacaat cgtcggcgta gactgcgccg
     7681 cgttcacagt ctgcccctct tccacgagaa tcgccaccac cgtgccgtcc atcgttgcgg
     7741 taatgcgcgt gtagcccaat tccgactcgg cggtattgat ggaaattttg ctctgtctga
     7801 ttagagcctt cagctcggca acattggctt tggcggcggc aagcgcatcc tgtgcgcttt
     7861 ccaaatcttc tttagcggtc gcatcatcct tccacaacgc cgcctgacgc ttatatttct
     7921 tctccgcgct gcccaatgca atctgtgccg acaccagctt cgcctgatac gtttccaact
     7981 tggatttttc cgtattgagc gtattggtct gcgaggtcga attgatttcc gcaatcaaat
     8041 cgcccttttt aacctgttgc ccgagtttga cataaagttt cttaatctgc cccgatgcct
     8101 gcgcgccgac cgataccagg ttggacggcg aaatctcccc tgttgcagaa accgtccggc
     8161 tgatgtcgcc gcgcctgacc gtttccgtaa tataagcagc ctgcggctcg ggcttcagat
     8221 aagaccatcc gccccaaacc gctgccgccg cgaccgccgc aacagccgcc catttcatca
     8281 tttttgccat gttttcaatt ctctgtatgc gtcaatccca taaatcggaa gcggattcta
     8341 ccgcaagtcc ctgccccatc caaggcggca aactgccgac aataaaaata accatttgtt
     8401 ttaaatggct tatacaatct gctttcccga aaatttcaat ccgtttacat attccgccat
     8461 ttccgatacg gcgttataat gccgtcgacc gacaaacaaa tggaaacaca ttgctatgaa
     8521 aaacgttcaa aaaggcttta cgctgctcga gctgatgatt gccgtcgcca tcctcggcat
     8581 cctgacgctc atcacctacc cttcctataa aacctacatc cggcgcgtcc gcctgtcgga
     8641 agtcaaatcc accctgctga tgaacgcgca aaacttagag cgttactacc gccaaaaagg
     8701 gacgtttgac aactacgacc aaaccaaact gaaacaaaac aagtatttca aaattacctt
     8761 aagcaaagtc aaccccgacc acttcaccct tcaggccgtc cccgatccga cgaccaacga
     8821 aggcgaaacc tgcatcgtca cgctcaacga cggcggcaca ctgtccgccg ccggcaacgg
     8881 gcagtcctgc ccggacttcg actaatcctc cgccgaagcc cgcaaacccg taaagatgcc
     8941 gtccggacac agcttcagac ggtatcttta cgggtttgcc catccggttt ttcaaacttg
     9001 aaacctgccg cccctcatcc ggcaaccgcc ccgatgccgc gcctttccaa gctgttataa
     9061 tataacatat aatctatatt tttcaacaac ataccacttg acctgtgaga tgcggaagcg
     9121 tatgcttacc cttatatcgg acaacattcc gtttacaggt ttttttgccg taactaagga
     9181 gcgcaatcat gaaaatgcag gcagttgttg tgaataaaaa tgtagcgggc gatgtggaag
     9241 taatcgaacg cgaggttcgc ccgttggaat acggcgaggc attggtcgaa gtcgaatatt
     9301 gcggcgtgtg ccacaccgac ctgcacgttg cggcaggcga ctacggcgaa aaaccgggcc
     9361 gcgtgttggg acacgaaggc atcggtttgg ttaaagaagt tgccgacggt gtgaaaaatc
     9421 tgaaagtcgg cgaccgcgtc agcatcgcct ggctgttcca aagctgcggc tcgtgcgaat
     9481 actgcaatac cggccgcgaa accctgtgcc gctctgtatt gaacgctggc tatactgccg
     9541 acggcggcat ggcgacccac tgtatcgtga gtgccgatta cgcggtcaaa gtcccagaaa
     9601 gcttggatcc tgcgcaagct tcgagcatta cttgtgccgg tgtaaccact tataaagcca
     9661 ttaaagtttc cggcgttcgt ccgggccaat ggattgcgat ttacggcgca ggcggtttgg
     9721 gcaacttggg ggtccaatac gcgaaaaaag tatttggcgc acacgtcgtc gccatcgaca
     9781 tcaacgatga aaaactggct tttgccaaag aaagcggcgc agatttggtt gtcaacgccg
     9841 ccaaagaaga cgctgccaaa gtgattcagg aaaaaaccgg cggcgcacac gctgcggtcg
     9901 taaccgccgt atctgctgcc gcattcaact ctgccgtgaa ttgcgtccgc gcgggcggac
     9961 gtgtggttgc catcgggctg ccgccggaat cgatggattt gtccatcccg cgtttggttt
    10021 tggacggcat cgaagtggtc ggctctttgg tcggcacacg caaagatttg gaagaagcct
    10081 tccaattcgg cgcggaaggt ttggttgtgc cgaaagtcca actgcgtgct ttggatgaag
    10141 cacccgccat tttccaagaa atgcgcgaag gcaaaatcac cggccgtatg gtgatcgata
    10201 tgaaaaaaga atgcagctgc ggtcatcacc actgatttga cgtggcgata cacatcgaaa
    10261 tgccgtctga acgctgttca gacggcattt tttatggatt tggatttgat tttaatccgt
    10321 tctgtttgaa atgccgtctg aaaacccgta tcgcaacacg tcattaagaa cggcaagatt
    10381 caaccgttct gcaacccgtt caaacggctt ccgccatttc caacgcttgt ttgatgtcca
    10441 cggcgacgac gcgcgagaca cctttttcct gcatggttac gccgacgagc tgctccgcca
    10501 tttccatggt caggcggttg tgggagatgt agaggaactg ggtttgcgcc gacatttctt
    10561 tgaccaggtt gcagaaacgc gaggtgttgg catcgtccag cggagcgtcg acttcgtcca
    10621 aaaggcagaa cggagcagga ttgaggctga acagggcgaa cacgaggctc atggcggtga
    10681 gggctttttc gccgccggaa aggaggtgga tggtgctgtt tttcttgccg ggcggacgcg
    10741 ccataatgga cacgccggcg gtcaggaggt cgtcgcctat cattttgagg gtggcttcgc
    10801 cgccgccgaa cagggtcggg aagaaagttt ggactttgcc gttgacggcg tcgaaggttt
    10861 ctttgaaacg cgctttggtt ttgtcgtcga tttgggcgat ggcttcttcc aaaatggtga
    10921 tggcggcctg cacgtcttcg ctctggctgc ggtagtagcc gtcgcgttcg cgcgcttctt
    10981 cgagttcttg cagggcggcg aggttgaccg cgccgagtgc ttcgatttgt tgcgaaaggc
    11041 tgccgatgct ggtgttcaat actttcggcg attctttcgc caacgcttcg agcgcgtcca
    11101 aatcggcggc gcgttcggtc aggttttgat ggtagcgttt ggcgttgatc agggcttcct
    11161 gctgctgcaa cagggcggtt tgggtagcgg cctgaagctg cggcagcttg gtttgcaggg
    11221 tttgcacttt agcgtattgc tccctgccct gcgcctgaat ctgcgcgagt ttttcctgta
    11281 cggcgatgta ctcttcatcc aaagtttgta cggcttcggt caactcgtcg agttttatgt
    11341 gctgctcgtc gttttggaac tcggtttcat aggcgagggc aagctcttgc tggcgttcct
    11401 gccaatcgag ggtttgctgt tcgagtcggg cgatttgctg ttggtagttt tgtttttgct
    11461 ggttgagctt gtggacggcg acttcggcaa gcccgtattg gcggttggct tccagcaggg
    11521 caagctgcgc ctgtttcaga cgaccttgct gctcttggcg gctgtgtgcg gtggtttgct
    11581 gctggtgttc gagttcggcg gcggcttcct gcaaaatggc gatgtcgtct gaaagcccgt
    11641 cggacgtgtg ttgcaacacg gtctgctctt ccgccaactg cgccagttcg cgctcgatgt
    11701 gttcgcggcg gatttgccct tggttggtgc gcgccagaag ttcggcggcg cgttgctgcg
    11761 cttggctgta ttggcgcgtg tgctgctgtt gctgctgcat caggttttta tgttgcactt
    11821 cggacgagcg cacggttgcc tccgcctgtt tgaacgcggc ttcggcggag gagagttcgg
    11881 gggagaggtt ttccagttcg gacgcgatgc cgtcgaggcg cgctttttgg gcaatcaggc
    11941 tttcctgcgc gggtttggca tagagcagga cgctgacctt atcgacctga tggccttcgg
    12001 gcgtgagcca gatttgatgt gcgcccaaat cgttttgatg cgcgagggcg taactcaaat
    12061 caggcgcaca caatacgccg tcgagccagt ggtgcaatgc cgtctgaaac ggcggctgcg
    12121 cttggatttg gttcagcaat gcctgtacgg gcagggattt tttgatgccg ccggagagac
    12181 cgtctgaaag ccacgccgcc tgcccctgcg gcaaaggttc gggcggtacg aaaccttgcg
    12241 gcacgtcgcg ggcgtgcagg cgttcggcaa ggatgacgga cagggcgtgc tgccactcag
    12301 caggcgcggt aatgtgttgc cacagttgcg gcgcggcggc gtggccggtt gcctgccaga
    12361 aatcggctgc ttcctgctgt tgcgacagga tttgcgacaa cgcctgctgc tgcgcctgca
    12421 aggtgatatg ttgctgcttc aggctttgga agcggtttga ggccgtctga aacgcctcgc
    12481 gggcggcgtg taaggcttct tcggcggcga tgatttgttc ttcgtaatgc tcttgccggc
    12541 tttgcaacaa ggcagcggcc tcctgcgcgg cggcggtttc cgcttcgtcg ggcaggttca
    12601 gggcttggtt ctcctgtttc agacggcctt tgcgctcttc gtgcttggca acggtttgtt
    12661 cggcatgggc aagctgctgc tgcttcaacg ccagttcgcg gcggatgcgg tttgcctcgt
    12721 cctgctgggt ttggaaggcg gcattgagcg cggtttgggt ttcttccaat tcgggcagac
    12781 gctcttcgtg ttcggcaacc tgcatcgccc attccgccag ctcggtttgc ttttcttcga
    12841 cctgcgactc gttttcttcc agcttgacgc ggatttgctg ctgctcctga tggatgcgtt
    12901 gcatctgcgc ctgcgctgcc tgcttgtcgc gttcgatgcg ttggtgcagg ttttgctgat
    12961 ggcggatttg ttcttccaaa cgggcaatct gctcgcgcaa cacgccgcgt ttgttgccca
    13021 gttcgtgtac cgcctgctgc tgcgattgtt cggtggtctg caaggcgtgt acttcgtcgt
    13081 ttaacgcctg aacctgcgcg gcggtttcgt cctgctgcgc ctgcaaagac tgatgctgcg
    13141 cggtcgcctt gtcggcggcg gcaagcgatt gctgccattg ggcgtaatcg agcaaatcct
    13201 gctgttggtt caactgcgcg gtcagggatt tgtagcgttc ggcggtttcc gcttgttttt
    13261 ccagcttttc cacctgacgc gccaactcgt tctgcaaatc gcccaaacgc tgcaaatgct
    13321 cgcgcgtgtc tttcagacga ccttccgtct ccttgcggcg ttccttatat ttggacacgc
    13381 ccgccgcctc ctcgatatag gcgcgcaact cctccggccg agcttcgatg atgcgcgaaa
    13441 tcatcccctg ctcgataacg gcataaccgc gcgcgcccac gcccgtaccc agaaacaaat
    13501 cagtaatatc gcggcggcgc acggtctgat tgttgatgaa atatgtcgat tcgccctgac
    13561 gcgtcaactg ccgcttgatg ctcacctcgg catactgccc ccacgccccc tgcaaactgt
    13621 ggtcgctgtt gtcaaacacc agctccaccg aagccctcgg cgcaggacgg cgcgtcgccg
    13681 caccgttaaa aatcacgtcc tgcatactct cgccacgaag ctgcttcgcc gaagcctcgc
    13741 ccaacaccca gcgcaccgcg tcaatcacat tcgacttgcc gcagccgttg ggcccgataa
    13801 ccgcgacaag ctgccccgga acatgaatcg tggtcgggtc ggtaaaagat ttgaagccgg
    13861 agagtttgat gtgggtcagg cgcataatgg tcggaaaaaa taaaaaagaa gcgtatttta
    13921 acggaaatcc cgccgcacca ccccatatct ttccggcaaa accttaccgc atcccgcacc
    13981 cgcgatgccg tctgaagcct tcagacggca tttttaacgc gcccgaaccc cggttccgcc
    14041 aatgcccgat gcttggcgcg gcatttgccg tattgccccg ccgccgggcg cgttataatc
    14101 cgccccgaca tccgcccgat tggaaacctg cctatgaaac ccgcctattt catctccgac
    14161 ctgcatttga gcgaaaagca ccccgaactg accgcgctgc tgctgcgttt tttacgttct
    14221 tccgccgccg ggcaggcgcg ggcgatttat attttgggcg atttgtttga tttttgggtg
    14281 ggcgatgacg aagtttccga gttgaatact tcggttgcgc gggaaatcag gaaattgtcc
    14341 gacaaaggcg ttgccgtgtt cttcgtcagg ggcaaccgcg acttcctgat cggtcaggat
    14401 ttttgccggc aggcgggtat gacgctgctg ccggattact cggttttgga cttgttcggc
    14461 tgcaaaaccc tcatctgcca cggagacaca ttatgcaccg acgacaaggc ataccaacgt
    14521 ttccgcaaaa tcgtgcatcg gaagcggctg caaaaactgt tcctgatgct gcccctaaag
    14581 tggcgcacgc gccttgccgc caaaatcagg cgtgtcagca aaatggaaaa acaggtcaag
    14641 cccgccgata ttatggatgt caatgccgcc tttaccgcgc ggcaggttcg cgcctttaac
    14701 gcggaaaggc tgatacacgg acacacccac cgcgagcata tccatcacga aaacggcttt
    14761 acccgcatcg ttttgggcga ctggcataac gactatgctt caatcctccg cgtagacggg
    14821 gacggcgcgg tattcgtgcc gctggaaaaa tactgaaaat gccgtctgaa gcctttcggg
    14881 cggctttttt tacacgcccc tttcccacac tccctccgcc gcatcatcac catccttaac
    14941 ccgcattaag gggcgttatc gataaaatgc ttgaccgatt acataagatt acgtaaagtg
    15001 tgcaaacgca taacaattgg tcttaccaat ttatctttta accaaaatat cgggtgtacg
    15061 ggatttgaac gtctttccaa acctcgacgc gccttatgat gcaaatgcct aaaaaggatt
    15121 cgttatggca cttttcctca gcatattccc catcgtcctg ctgatttggc tgatggtgaa
    15181 aaaaaacagt atgccctcct acgtcgcgct gccgattacc gcagtgctga tttacgccat
    15241 caaacttttc tacttcggcg atgcgggcat gctgctcaac gccaccgccg cttccggcct
    15301 cgtcaaaacg ctcacgccga ttaccgtgat tttcggcgcg attatgttca accgtatgat
    15361 ggaaaccacg ggctgcatcg atgtcatccg caaatggctg gcgaccatca gccccaaccc
    15421 cgtagcgcaa ctgatgatta tcggctgggc ttttgccttt atgattgaag gcgcatccgg
    15481 cttcggcacg cctgccgcga ttgccgcgcc gattctgatg agcttgggct tcaacccgtt
    15541 gaaagtggcg attttcactt tggtgatgaa ctccgtcccc gtctctttcg gcgcggtcgg
    15601 tacgccgacc tggttcggtt tcgcaccgct gaacctgagt gccgaagaca tcctcgccat
    15661 cggcaggcag accggcgtaa tgcacttctt cgcaggtttc gtcatccccg tcatcggctt
    15721 gggcttcatc gtaccttggt ctgaaatccg caaaaacttg ggcttcgtcg ccattgccgt
    15781 cttctcctgc accattcctt atgtcgcatt ggcgatggtc aacgaagaat tcccgtcgct
    15841 cgtcgccggc gcgatcggcc tgatggtgtc cgtattcgcc gccaaccaag gctggggctt
    15901 gagcaaggac cacgccaaag acccgaatgc cgaaaaagtg ccgttcgccc aagtcgccaa
    15961 agcactcgcc cctttgggta tgctgatcgg catgctggtg gttacgcgca tcaaacagct
    16021 cggtatcaaa ggcattttga ccagcaaaga agaatggttc agcttccaac tgccgtttga
    16081 tttgtccaaa atcaccgtca gcgactccct gacgattacc ttcggcaata ttttcggaca
    16141 agatgtcagc gcgtcttacc aaacgctgta cgtcccggct tggattccgt ttgtgctgac
    16201 cgtttggatt tgcatcctgc tgtataaaac caaattcaaa gatgcctgga cgatttatgc
    16261 cgtaaccttc aatcaaacca aaaaaccgct gcttgccctg atgggcgcgc tgattatggt
    16321 tcagctgatg ctggtcggcg gcgacaattc gatggtgaaa atcatcggta aagaatttgc
    16381 cgcaatggcg ggcgaacact gggtttattt ctcgccgtat ctgggcgcga ttggcgcgtt
    16441 cttctccggt tccaacaccg tgtccaacct gaccttcggc ccgattcagc agcaaatcgc
    16501 cttggatacc ggcctgtccg ttaccctgat tctggcgttg cagtccgtcg gcggcgcgat
    16561 gggcaatatg gtgtgcctca acaacatcat cgccgtatgt accgtattgg atgtgaaaaa
    16621 ttccgaaggt gcgattatca agaaaaccgt tatcccgatg gcgatttacg gcgtgattgc
    16681 cgtcgtcgcg gcaatgattt tcttcctcta ggcgaaacgc caccatccat agcggcagca
    16741 cgcggggaac ggaaaccgcc gttccccgtc tgggcggcgc gtcgggcaga atcccggtgc
    16801 cggaacaagc ccgccccgcc aaacaaatgc cgtctgaaac cggaaaaggc ttcagacggc
    16861 atttttccgc cgcttgcgtt caggcgtaat gatgatgcgg catcttgccg tcaaggcggt
    16921 agcgcgtgcc gcaatacggg caggcaacgc tgcccgattc tccttcgcac aaaggcaaaa
    16981 acactctcgg atgcccgttc cactgctcgt tgtcgggtcc cgagcaatac agcggcagat
    17041 tttccggcaa cacggaaatt tcctgcagat tcagattgtc catttgattt tcctttgcgt
    17101 ggttggcgtg cgcccatttt acgccatcgg caggctaaag gatattttcg gcgcaaagcc
    17161 gcaatccgct ataatcccca cttttcagac ggcataccat gactgcgctt acccttccgg
    17221 aagacatccg ccaacaagaa ccatccgttc tgctctatac cctcgtttcc gcctacctcg
    17281 aacacaccgc ccaaaccggc gacgaatccc tctcctgcct gtccgacgac cagcacacgc
    17341 tgaccgcatt ctgctacctc gacagccaag tcgaagaagg cggcttcgtg caactcatcg
    17401 cctccggtta cggcgaatat attttccgca acccgcttgc cgacagcctg cgccgctgga
    17461 aaatcaaagc cgtgccgaaa gtcttggaca aagccaaagc cctctacgaa caacacggcg
    17521 aaaccatcga aacgctcgcc gacggaggcg cagacatcga atccctgcgc aaacagttcc
    17581 ccgactttga agaatgggac ggcgcatact acgaagccgc cgaacaagac ctgcccctgc
    17641 ttgcagaaca catcctgtca aactgggaaa ccttcgccca tatcgggcag gcgtgattgc
    17701 gtctgtttcc agcccgtgta aaacagcgta aaatcggcaa acccgaatga ccttccgtcc
    17761 ccatcaagga gcaagctatg ttcttcaagc acatcgaagc cgccctcgcc gatccgattc
    17821 tcggtttggg cgaagcattc aaagccgaaa cccgccccga aaaagtcaac ctcggcatcg
    17881 gcgtttataa agacgcatcc ggcgcgacac ccattgtcaa agccgtcaaa gaagccgaaa
    17941 aacacctgtt ggaaagtgaa accaccaaaa actacctcac catcgacggc gttgccgatt
    18001 acaacgcgca aacccaaatc ctgctgttcg gcaaagacca cgaaatcatc gccagccgcc
    18061 gcgccaaaac agcgcaaagc ctcggcggta cgggcgcatt gcgtattgcg gccgagtttg
    18121 ccaaacgcca gttgaacgcg caaaccatct ggatttccaa tccgacctgg ccaaaccaca
    18181 acgccatcgc caaggcggtc ggtatccaag acaaacctta tcgttattat gatgccgcca
    18241 aacacggttt ggattgggac ggtatgattg aagacttgag ccgggcgcaa aaaggcgaca
    18301 tcgtcctgct gcacggctgc tgccacaatc ctaccggcat cgaccctacg cccgaacaat
    18361 gggaaacttt ggcaaaactt tctgccgaaa aaggctggtt gccgctgttt gactttgcct
    18421 accaaggctt cggcaacggt ctggaagaag atgcctacgg cttgcgcgtg ttcttgaaac
    18481 acaatacaga attgctgatt gccagctctt attccaaaaa cttcggtatg tacaacgagc
    18541 gcgtcggcgc gttcactttg gtggccgaag acgaagaaac agcaatccgc gcacacagcc
    18601 aagtcaaaac catcatccgt accttgtatt ccaacccggc ttcacacggt gcgaacacca
    18661 ttgcgctggt gttgaaaaat gatgatttga aagcccaatg gattgccgaa ctcgatgaaa
    18721 tgcgcggccg catcaaagcc atgcgccaaa aatttgttgc gttgctcaaa gccaaaggtg
    18781 caagccaaga ctttgatttc attatcgaac aaaacggtat gttctctttc agcggtctga
    18841 ctcccgaaca agttgaccgt ttgaaaaacg agtttgccat ttatgccgtc cgctccggcc
    18901 gcatcaacgt tgccggcatt accgacgaca acatcgatta tctgtgcgaa agcatcgtga
    18961 aagtactgta aaaagcacag ccatatgaaa atgccgtccg aaccttaacc agttcagacg
    19021 gcatctgttt tttgttaaaa aacaataaac cataacaatt tcaaacaagc taaaaacatc
    19081 tataaaatca acccaatatg atgaactcaa ataaaaaata cgaatatcat cattctcaaa
    19141 ttcaaaattt gaacgccaca gtattatccg aagccacaaa aacctttccg ccgtcattcc
    19201 cgcgcaggcg ggaatccggg aatccaatgc tgcaagaatt gattagaaac aactgaaacc
    19261 gagcaaactg gattcccgcc tgcgcgggaa tgacgaaata aggcgagtga aactgatgaa
    19321 acaaattgat taaaaacaat aacttatatt attttattta aattttcgat aaaatcaatt
    19381 ttccgtattc aatactgctc caatcaattc ccgcgcaccg tcgtccgcca atttcttagc
    19441 gacagcgcgt ccgagcgcgt cggcatattc ggcaggggct tgcgcgtccg cccgcaacac
    19501 aaccgaaccg tcgggatgtc cgaccagtcc gcgcaaggtc agcaagccat tttcttccgt
    19561 gcaatatgcg gccaaaggca cttggcagct tccgcccaaa gcgcgtgcga gggcgcgttc
    19621 ggcggtaacg caggcattgg taacgccgtg gttcaagggt ttcaaaactt catacaaatc
    19681 ttcgcggtgc gcggcaattt cgatacccaa tgcgccttgt ccggcggcag gcaggctgtc
    19741 ggattccgac aaaatcatgc ggatgcgtcc gtccaatttc agacgctgca aaccggcggc
    19801 agccaagata attgcgtcgt attcgccgtt atcgagtttg gacaaacggg tttgcacatt
    19861 gccgcgcaaa ggtttgataa gcaaatgcgg atagcgcgca cgcaactggg cttcgcggcg
    19921 caggctggat gtgccgacaa ccgcgccttc gggcatttct tccaaacgcg tgtattggtt
    19981 ggacacaaac gcgtcaaacg gattggcgcg ttcgccgatg gcggcaagcg cgaaaccttc
    20041 aggcaaatcc atcggcacgt ccttaatcga atgcaccgcc aaatcggcgc gcccgtcata
    20101 taaagcctgt tccaactctt tgacaaacaa gcctttaccg ccgacttttg acaaagtttt
    20161 gtccaaaatc tgatcgccgc gcgtggtcat gcccaaaatc tcgacttcgc aatcgggata
    20221 cagagccttc agacggcctt ggatatgctt tgcctgccac atagcaagca ggctttcgcg
    20281 gctggcgatg acgagttttt tcgggttcat gttcaatgcg ttcaaagaat ggaaacggcg
    20341 tgaagtctat catattttga acgtttcttc agacgacacc gtcaggtcta tgttatattt
    20401 acacccacaa aacacgaaac ccgccacaat gaaacgcacc aaaaaatacc ctttcttaac
    20461 gttgcagcgg caacgtttcc atttgaactt tgaaaacgcc tcttccgccg ccggcatccc
    20521 agccgaacgc gatttctacc gctgggcgtg gtctgccttg aaaaatgaat accgccgcgc
    20581 cgacatcagc ctgattcttc tggacgaaga agaagcccga gcctacaacc gcgactaccg
    20641 cggcaaagat tacgccacca atgtattgag tttcgcgctc aacgaaggcg aaatcctgcc
    20701 ctgccaagtt tcggaaaaac tgtacggcga tttgattatc tgcccgcaag tggttttgaa
    20761 agaagccgcc gaacaaggca aaacacccga gcagcatttt gcccacctga ccatacacgg
    20821 tactttacac ctgatgggct acgaccacat cgaagacgac gaagccgaaa taatggaagc
    20881 cgaagaaatc cgcctgatgc gggcggcagg ctatcccaac ccctaccgag aggacggaca
    20941 ttaaaatgga cggcgcacaa ccgaaaacaa atttttttga acgcctgatt gcccgactcg
    21001 cccgcgaacc cgattccgcc gaagacgtat taaacctgct tcggcaggcg cacgagcagg
    21061 aagtatttga tgcggatacg cttttaaggt tggaaaaagt cctcgatttt tccgatttgg
    21121 aagtgcgcga cgcgatgatt acgcgcagcc gtatgaacgt tttaaaagaa aacgacagca
    21181 tcgagcgcat caccgcctac gttatcgata ccgcccattc gcgcttcccc gtcatcggcg
    21241 aagacaaaga cgaagttttg ggcattttgc acgccaaaga cctgctcaaa tatatgttta
    21301 accccgagca gttccacctc aaatccattc tccgccccgc cgtcttcgtc cccgaaggca
    21361 aatcgctgac cgccctttta aaagagttcc gcgaacagcg caaccatatg gcgattgtca
    21421 tcgacgaata cggcggcaca tccggcttgg tcacctttga agacatcatc gagcaaatcg
    21481 tcggcgaaat cgaagacgag tttgacgaag acgatagcgc cgacaatatc catgccgttt
    21541 cttccgaacg ctggcgcatc catgcagcta ccgaaatcga agacatcaac accttcttcg
    21601 gcacggaata cagcagcgaa gaagccgata ccatcggcgg cttggtcatt caagagttgg
    21661 gacacctgcc cgtacgcggc gaaaaagtcc ttatcggcgg tttgcagttc accgtcgccc
    21721 gcgccgacaa ccgccgcctg cacacgctga tggcgacccg cgtgaagtaa gcactgccgt
    21781 ttctgcacag tttaggatga cggtacgggc gttttctgtt tcaatccgcc ccatccgcca
    21841 aacataaaaa tgccgtctga aaccgttgca agtttcagac ggcatttcgc ttatccgctt
    21901 attttttgct caaagtcagc cccgtccaac cgaataacag ggtcaaagcc aaactcaaat
    21961 agcagaaaaa ggcatacggc agatattccc aaaccggcac acccagcgcg tggctgatga
    22021 acacgccgca tacgctccac ggtacgagcg ggttaatcac cgtccccgca tcttccagcg
    22081 tccgcgacag attgcgggaa tgtagtccga gcctgtcgta aacgggttta aacgtttctc
    22141 cagaaagcag gatgctcaaa tattgttcgc caatcaggaa attaaccccg accgaagtca
    22201 tggcaacgct gaacgtcgcg cgtccggcat tcgtcaagaa ggtacggatg gcctccaaca
    22261 gggaaggaat cacaccgagc gcaaacaaca gtccgcccaa actcatcccg agaatcacga
    22321 tggtttgcgt gaaaaacata ctttccaaac cgccgcgcga aatcagtttg acaacatctt
    22381 taaacgcttc gccttcgagt ttgtagccgc cgtaaaacca cgcaccgagc tgacgcagat
    22441 cgggcgtgct gtgcagatac gttacagcaa cggcaaccat gacggtaaag agcatggcga
    22501 cgacggcgtt gatgcgcatc aatgccaaaa cgaccaacag cgcaaacgga atcagcgaat
    22561 agcagtgcac caatcccgtg gcttcaagct ggctgcggaa ggattcgacg ctgttcaaat
    22621 cctgcgcggc gacgctggga agaagccaca gcatcagtgc cgcactgatg agccacgcgg
    22681 gaatggtggt gtacatcata tttttgatgt gttcaaacag gtcgataccg acaatggacg
    22741 cggaaatgcc cgtcgtatcg gaaagcgggg acattttgtc gccaaaaaat gcgcccgaaa
    22801 caatcgcgcc cgccgtcatc gccatatcgg cctgaaacgc cgccgccata cccataaagg
    22861 caacgccgac agtggcgcag gtggtcaggc tgctgccgat ggacacgccg atgacggaac
    22921 acagcgcgaa ggcggaaaaa taaaaataag tcggggaaat cagtccaaaa ccgtaataca
    22981 tcagcgtcgg aatcgcgccg ctcatcatca gcgcgctgac catcagcccg atgaagaaaa
    23041 acaggtaaat cgcgcccata ccctgattca acgcgcctat catgccctgc tgcatatcgt
    23101 tgtatttcaa accgcgcgcc aagccgtaca aaatcagcac gacgatggcg gcaataatgg
    23161 acatatgcgg cagccactcc aatgaaatga tggtatagcc catcgcggca atcagagcga
    23221 cgacgacggc aagtgcctca ccgcgcggca tatcgagtaa ggatttgaaa gcgaacatga
    23281 ttctttgccc gatttgatat aaagttaacg gattctaaca caccataccg aatccgcccg
    23341 acgttttcag acggcatttt agcgttttac aaaagccgga cagaaacaaa acggcaattt
    23401 tctcaattta aattaatttt tttgaaatat aattttttat ttttttgttt gttgacatat
    23461 aagactttta tggattgaag tattaaaacc tcggcacaca cgccgcaaac accggtacgc
    23521 aaaaatgccg tctgaaagat gttcagacga catttctcaa gaaaaagatt atttgtccgc
    23581 cctatatccg tacaccgcaa aaaacacgat atacagataa cagatgacgg aaacgacaaa
    23641 cgaagacatc agggtgtaag tatctgccac ccagccctgc acgacaggaa cgaccgcacc
    23701 gccgacaatc gcggtacaca gtacaccgga agccgcgttg gtaaattttc ccaatccttt
    23761 ggttgccaaa gagaaatcgt cgggaacata atcgagttga aaaaaccgat ggcaagcagc
    23821 gaccacatcg ccacatccgc attgccgcta cccgtcgcca tcgcgacggc aagcagtacg
    23881 accgcagccg atgcgttaaa cgccaaataa cggttgggcg cgaatttcgc catcaccgcc
    23941 gaaccgagga aacgtccgac catcgcgccg ccccaataga acgacaggta atgcgcggca
    24001 gaagcatgat ccagcccttt cagataaccc aatacgttga ccatcaacga accgatagac
    24061 acctccgcgc cgacatagca gaaaatgcct gccgtaccga acacgagatg tttgtattgc
    24121 catacgctgg ttttgccgtc gtggttgtgc gcgctttcct cggcggcaat tttgcgcgcg
    24181 tcgggcagcc ggatcatttt cacgaaaacg gcaaggataa tcagcagccc cgccagtccc
    24241 aaatagggaa tctgtacgga agaaatctgt tccgccttgc tgacggtttg ggttgcgtcc
    24301 gccagaatca ggaacgcgcc gatttgcggc gcaatggtcg tacccaaagc gttaaacgcc
    24361 tgaaccagcg tcagtgttgc cgattccttg ccgggtttcg ccagcagggt aacataagga
    24421 ttaccggcga cctgaagcag cgttacgccg gaagccaaaa taaacaacgc gcccaaaaat
    24481 accgcgtaag aatggctacc cgcagcagga taaaacagca ggcatccgac cgccgtcagc
    24541 agaaagccgc cgataacgcc gtttttgtag ccgactttgc cgacaaaagc ccccatcggg
    24601 atggacatca ccgcataggc ggtaaagaaa cagaattgga tcagcatcgc ctgaacgtaa
    24661 gacaggtcga aaatttcttt caaatgaggg ataaggatgt cgttcatgca ggtaataaaa
    24721 cccatcatga agaacagcgt ggtcaagacg accaatgggg acgtatgatt gttttgtgat
    24781 tgcgcagaca tgatttgcct ttcgtggtgt cggaatatta cccgtttcaa gaaaatagaa
    24841 attgacattt cttaacggca atgcaaatca tactgatatt tgaacatttt gcaaacttta
    24901 tcggatgcag gcaatgcgcc gatctgaaat tcaagcagca gaaattaata ttttacagtt
    24961 gcttgcatag attttaatag attttaaaaa attccggcgg acttgcaaaa accgttccca
    25021 ataaaatgaa aatccgccgc cgtatccgga aaattcggaa atgccgcatc gtggcatcaa
    25081 aaccggagcg gaaagaatgt cgtatgcccg acaaaaaagg ggcatacggc tgtgccgtat
    25141 tttttgcacc gtcctccgcc cttcggcggg ggcgtgaaaa tatagtggat taacaaaaat
    25201 caggacaagg cgacggagcc gcagacagta cagatagtac ggaaccgatt cactcggtgc
    25261 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt
    25321 tgttaatcca ctataccgta caatccgtcg ccgatttttg cctgtttgag actgtttgca
    25381 atgatgcccg cctacgccta cgccgcgctc gctttttccg ccttcacttc cgccaccctc
    25441 ctgcccggca catccgaagc cgcctttgcc ctgttcgtcc accgctttcc cgaacacgca
    25501 tacggcgcgt tgctgtgcgc cggccttgcc aacggattgg gcagtatggt ttcctattgg
    25561 atggggcgtt tgctgccctc ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg
    25621 cgtttcggta tttggctgct tgcgtttgcc tggctgcccg tcgtcggcga cgcactgccg
    25681 cttgccgccg gctggctgcg gttgaatccg tggacaagcg ggctgatgct ggttatcggc
    25741 aaaacggcgc gttacgcctt cattctgtgg ggaatgcaat attacgccgc ctgaaccata
    25801 cgttataatg ccgaacacac aaacccaccg tataaaaaaa tgaacagaca catccgccaa
    25861 gaaatcttcg aacgcttccg cgccgccaac ccccatccga ccacagaact caacttcaac
    25921 tcccctttcg agcttttaat cgccgtttta ctgtcggcac aggcaaccga cgtcggcgta
    25981 aacaaagcga cggcgaagct gtttccggtt gccgatacgc cgcaggcgat gctggatttg
    26041 ggtttggacg gcgtgatgga atacacgaaa accatcgggc tgtataaaac caagtccaag
    26101 cacatcatgc aaacctgccg catcctgctg gaaaaataca atggcgaagt gccggaagac
    26161 cgcgaggctt tggaatcgtt gccgggcgtg gggcgcaaaa cggcaaacgt ggtattgaac
    26221 acagcgttcg gacaccccgt tatggcggtc gatacgcata ttttccgcgt gtccaaccga
    26281 accaaaatcg cccccgggaa agatgtgcgc gaagtcgaag acaaactgat gcgcttcatt
    26341 cctaaagaat ttctgatgga cgcgcaccac tggctgattt tgcacggacg ctacacctgc
    26401 aaggcactca aaccgcaatg ccaaacctgc atcatcaacg atttgtgcga atatcccgcc
    26461 aaagcctgac cgccgccgcc cttcaaaatg aaacgcttga aaattcggac ggacgttgta
    26521 gaatttcaac ctatgccgtc tgaagccgac aaaatgcgct tcagacggca ttaaagccgg
    26581 taccggcatc cgacaaagga caaaacgtgt tcaaaaaata ccaatacctc gctttggcag
    26641 cactgtgtgc cgcctcgctg gcaggctgcg acaaagccgg cagctttttc ggtgcggaca
    26701 aaaaagaagc atccttcgta gaacgcatca aacacaccaa agacgacggc agcgtcagta
    26761 tgctgctgcc cgactttgtc caactggttc aaagcgaagg cccggcagtc gtcaatattc
    26821 aggcagcccc cgccccgcgc acccaaaacg gcagcagcaa tgccgaaaac gattccgacc
    26881 cgcttgccga cagcgacccg ttctacgaat ttttcaaacg cctcgtcccg aacatgcccg
    26941 aaatccccca agaagaagca gatgacggcg gattgaactt cggttcgggc ttcatcatca
    27001 gcaaagacgg ctatattctg accaatacgc acgtcgttac cggcatgggc agtatcaaag
    27061 tcctgctcaa cgacaagcgc gaatataccg ccaaactcat cggttcggat gtccaatccg
    27121 atgtcgccct tctgaaaatc gacgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc
    27181 ccaaagattt gaaaccgggc gaatgggtcg ccgccatcgg cgcgcccttc ggcttcgaca
    27241 acagcgtgac cgccggcatc gtgtccgcca aaggcagaag cctgcccaac gaaagctaca
    27301 cacccttcat ccaaaccgac gttgccatca atccgggcaa ctccggcggc ccgctgttca
    27361 acttaaaagg acaggtcgtc ggcatcaact cgcaaatata cagccgcagc ggcggattca
    27421 tgggcatttc cttcgccatc ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa
    27481 acaccggcaa agtccaacgc ggacaactgg gcgtgattat tcaagaagta tcctacggtt
    27541 tggcacaatc gttcggtttg gacaaagccg gcggcgcact gattgccaaa atcctgcccg
    27601 gcagccccgc agaacgtgcc ggcctgcggg cgggcgacat cgtcctcagc ctcgacggcg
    27661 gagaaatacg ttcttccggc gaccttcccg ttatggtcgg cgccattacg ccgggaaaag
    27721 aagtcagcct cggcgtatgg cgcaaaggcg aagaaatcac aatcaaagtc aagctgggca
    27781 acgccgccga gcatatcggc gcatcatcca aaacagatga agccccctac accgaacagc
    27841 aatccggtac gttctcggtc gaatccgcag gcattaccct tcagacacat accgacagca
    27901 gcggcggaca cctcgtcgtc gtacgggttt ccgacgcggc agaacgcgca ggcttgaggc
    27961 gcggcgacga aattcttgcc gtcgggcaag tccccgtcaa tgacgaagcc ggtttccgca
    28021 aagctatgga caaggcaggc aaaaacgtcc ccctgctggt catgcgccgt ggcaacacgc
    28081 tgtttatcgc attaaacctg caataaccca cataatttaa ccaaaatatt ttttaaatga
    28141 taaaatgccc gtcaataccg attaacgggc attttacaaa aatcagttgg ttgattttaa
    28201 acaaatattt tttacgacaa aaaatatttc cgcccatttt gccaacaaaa cccaaaccgg
    28261 aggcggacag atacgctccc atccggacgc ttaatacccc cataaccatg cagctgatcg
    28321 actattcaca ttcatttttc tcggttgtgc cacccttttt ggcactggca cttgccgtca
    28381 ttacccgccg cgtactgctg tctttaggca tcggtattct ggtcggcgtt gcctttttgg
    28441 tcggcggcaa ccccgtcgac ggtctgacac acctgaaaga catggtcgtc ggcttggctt
    28501 ggtcagacgg cgattggtcg ctgggcaaac caaaaatctt ggttttcctg atacttttgg
    28561 gtatttttac ttccctgctg acctactccg gcagcaatca ggcgtttgcc gactgggcaa
    28621 aacggcacat taaaaaccgg cgcggcgcga aaatgctgac cgcctgcctc gtgtttgtaa
    28681 cctttatcga cgactatttc cacagtctcg ccgtcggtgc gattgcccgc cccgttaccg
    28741 acaagtttaa agtttcccgc gccaaactcg cctacatcct cgactccact gccgcgccta
    28801 tgtgcgtgct gatgcccgtt tcaagctggg gcgcgtcgat tatcgccacg cttgccggac
    28861 tgctcgttac ctacaaaatc accgaataca cgccgatggg gacgtttgtc gccatgagcc
    28921 tgatgaacta ttacgcactg tttgccctga ttatggtgtt cgtcgtcgca tggttttcct
    28981 tcgacatcgg ctcgatggca cgtttcgaac aagccgcgtt gaacgaagcc cacgatgaaa
    29041 ctaccgtttc agacgctacc aaaggtcgtg tttacgcact gattattccc gttttggcct
    29101 taatcgcctc aacggtttcc gccatgatct acaccggcgc gcaggcaagc gaaaccttca
    29161 gcattttggg ggcatttgaa aataccgacg taaacacttc gctggtattc ggcggcactt
    29221 gcggcgtcct tgccgtcgtt ctctgcacgc tcggcacgat taaaaccgcc gactatccca
    29281 aagccgtttg gcagggtgcg aaatctatgt tcggcgcaat cgccatttta atcctcgctt
    29341 ggctcatcag tacggttgtc ggcgaaatgc acaccggcga ttacctctcc acgctggttg
    29401 cgggcaacat ccatcccggc ttcctgcccg tcatcctctt cctgctcgcc agcgtgatgg
    29461 cgtttgccac aggcacaagc tgggggacgt tcggcattat gctgccgatt gccgccgcca
    29521 tggcggtcaa agtcgaaccc gcgctgatta tcccgtgtat gtccgcagta atggcggggg
    29581 cggtatgcgg cgaccactgc tcgcccattt ccgacacgac catcctgtcg tccaccggag
    29641 cgcgctgcaa ccacatcgac cacgttacct cgcaactgcc ttacgcctta accgttgccg
    29701 ccgccgccgc atcgggctac ctcgcattgg gtctgacaaa atccgcgctg ttgggttttg
    29761 gcacgacagg cattgtattg gcggtgctga tttttctgtt gaaagataaa aaacgcgcca
    29821 acgcctgacc cctttccctg ttcagacggc atatgccgtc tgaagctttt tggagcaaac
    29881 cgcaatgacc gtcccccata ttccgcgcgg ccccgtcatg gcagacattg ccgccttccg
    29941 cctgaccgga gaagaaaaac aacgcctgct cgatcccgcc gtaggcggca tcatcctctt
    30001 ccgccgcaac ttccaaaaca tcgaacagct caaaacactc accgccgaaa tcaaagccct
    30061 ccgcacaccc gaactcatca tcgccgtcga tcacgagggc ggaagggtgc aacgtttcat
    30121 cgaaggcttc acccgcctgc ccgcaatgag tacgctcggc gagatttggg acaaagacgg
    30181 cgcgtccgcc gccgaaaccg ccgccggaca agtcggccgg gttttggcaa ccgagctttc
    30241 cgcctgcggc atcgacttgt ccttcacgcc cgtcttagat ttggactggg gaaactgccc
    30301 cgtcatcggc aaccgcagtt tccacagtaa ccccgaagcc gtaacccgcc tcgccctcgc
    30361 ccttcaaaaa ggtttggcaa aaggcggtat gaaatcctgc ggcaaacatt ttcccggaca
    30421 cgggtttgtc gaaggggaca gccatttggt gctacccgaa gacgggcgca gcctcgacga
    30481 actcgaagcc gccgacctca ttcctttccg cattatgagc cgcgaaggta tggcggcggt
    30541 tatgcccgcc cacgtcgttt atccacaagt ggacacaaag cccgccgggt tttccgaaat
    30601 ctggctgaaa cagattttgc gccgcgacat cgggttcaaa ggcgtgattt tctcggacga
    30661 tttgactatg gagggcgcgt gcggggcagg cggcatcaaa gaacgcgccc gcatttcctt
    30721 tgaggcaggt tgcgacattg tcctcgtctg caaccgtccc gaccttgtgg acgaactgcg
    30781 cgatgatttc cgcattcccg acaatcccgc tttggcgcaa cgttggcaat acatggccaa
    30841 cacgctcggc agcgccgccg cgcaagccgt tatgcagacg gcggatttcc aagcggcgca
    30901 agcctttgtt gccgcccttg cctcgccgca ggatacagcg ggcggcgtaa aagtcggcga
    30961 agccttttaa atccgcccga cacgcggcaa aaaggcaaaa cggtataggc acgccgtatt
    31021 ttttgcgcta taatcacgcc ttacttaatt cggaatgtag cgcagcccgg tagcgcactt
    31081 cgttcgggac gaaggggtcg gaggttcgaa tcctctcatt ccgaccaaat ccaaaccgcc
    31141 tcggcatcca ttgccggagc ggtttttcct tttggcgtca acagaaaaat gccgtctgaa
    31201 acagcattct gtttcagacg gcatttcgtt ttccctaacc cttctatttg cgtgcactga
    31261 caaagggtat cggcaaggtt caaaagacca tcggaattta ggaagccggg cttgtaccca
    31321 acggcagcat cttattcagt aaggctccgt cctgattggg cggtggaaat accggcattt
    31381 ccacgcagaa gcggcacact ttccccgtat gtcaaacatc ggtaaaacgg tacgccggcc
    31441 ctttgagtta cactgcttaa actactctat tcccgcccat ttgtgcgtct gcacgctcaa
    31501 ctgccaatgc acggatgcgt cggggcgact gtttaaaata ccgatttggc ggatggtgtc
    31561 gtagatgttc atcgcaccgt cttgctcaca gggcgaaagg tagtaatgat gtgcgcggat
    31621 tttgcgttcc atgttttcgc agaacgcaag gacatcacca tcggcaacaa tccgcacttc
    31681 gtcggctgtt tcgatacagc tattttcata tttggcggcg tagcaggctt tggggctggt
    31741 ggcgacgtag tcgatttgcg gcggcgcggg attgagtccg ttggtttcga gacagaggaa
    31801 atagccttcc gccttgagcg tgtccagcag catatcgaga tgcggctgta tggtcggttc
    31861 gccgccggtg atgatgatgt tgcgggcggc ataggttttc agacagccta agatgtcgga
    31921 caggctcatc atgccgaatg tcaaataatc ggtatcacac cagccgcagg cgaggttgca
    31981 tttgcccaag cggacgaaaa cggcgggcat gcccgtgttc cagccttcgc cttgcaggct
    32041 ttcaaagatt tcgacgatgc ggtattgggg attttcgggg gcaacattga tttttttcat
    32101 accgccaaca ccgccgccgc tgccaaaaat gcgggcaaac cttgtttgac gagtatgcct
    32161 ttgttgccgg aagagaacgc gccccacgcg gcggcaatca ggacgaagcc gagaaacagt
    32221 accgtcacgc cgtacaccgc gttatccggc gcagcaaacc gcgaccacac cagcccgatg
    32281 ccgagaaagc cgttatacag cccttggttg ccgaacaggg tctgcacgcg attttgttcc
    32341 ataaattcat aaggcagctt gaacgtttcc gccgcttttt cgctgggaat ctgtgtcatt
    32401 tcaagccagg cgatgtagaa atgttcgacg gcgacgaaga ggactaggag gatgaagagg
    32461 agtttcatgg gtaagcacct taatttgcac taagatttat atttaacaaa ctttaccaat
    32521 tttatttaac aaaaaagcca cccattatgg atggcttctc taccaaaact tttcatgcta
    32581 cttttttcat ggttgcagct tttgctttat aagctcgttt ttcaatttgt ctttgaatag
    32641 ctgcaagttg cttcgttaat gcagcaatgc gagcgaaacg gatatccatt gatttcataa
    32701 ataactcctt gctgttgtta ataaaggctc gactgattat ttttgagcat agaacgattc
    32761 cgagaacatt cctcagtatc tttatttcta acgttagtaa tcatagattg tgcgtagaga
    32821 agtccttgag ccaaactaat ttttttgttt tccaaagaaa aatcattggg actgatcgga
    32881 agtttatcaa cctcttgttg taaatttttc acaatggatt ccaaagtatc caaatcagga
    32941 tcaactaaat catgcatacc atcaatccca aaatcatcgt acagtttcaa aagaaattgt
    33001 acgaacttat taattacatc tatgctctcg gcaataatgc cttcctcttg aatcatacta
    33061 ctagttgtat tcatggttat tatgcctgaa attttctatt acggtcgatt ttttagaaag
    33121 tatggaattt tacaattgat tatatgacaa gtcaaaacaa atctttaaaa tttgtttcaa
    33181 tattactccc cttcatactc cgcacacgat gtcggcgttt cccacaattt gacgcggcac
    33241 acgttcagcc ccgcgttttt cagacggtcg tacatttcga ccgccatatt ttcggcagtg
    33301 gtgcggcagg gcaggcgcag ggttttcatg ttccagccct ccaaaagcgc ggcgatttgg
    33361 cattcgcggc tgttgccgcc gtggtagatg aaggcgtggt cgaaggggtc ggtaatgtgt
    33421 tgtttgacaa tggctttcaa gtcggtaaag tccatcacca taccgtcttt cgcgccgcct
    33481 ttgataatgc cgtctgaaac ggtgatttcg agtttgtagg tatgtccgtg caggttttgg
    33541 catttgccgt catgcccgtc gagcatatgc gaggagtcga aggtgaagat tttggtgatt
    33601 ttcatgggtt ctcaaatggg caaatagcct tgaaagccga tttcggcggg tttgccgtgc
    33661 ggattcgggg tcaggcggat atggaaattg ccgatgcggt cgagcgcaag caggttgtcc
    33721 acgtctttta agttgtcttt atgttgctga acatagctgt cggtcaacag ggtttgcagg
    33781 gctttttgtt tggcttcggc aggcgtggcg gcggcgaaca gcccaaaagc gtgcgcttcg
    33841 gcgagctgcc ctgcgcgata accgccgaca ttggcgaaat acaggcgcgg ggcgtgttca
    33901 gacggcattt tcgttttggg cgtttcggat acggcgatgt cgtaaccgtc cgcccattcg
    33961 acaacctgcc agccgtcgat gtggattttg tccgcatcgc cgaaccacgc ggctttgagt
    34021 gcggggacgg cttcacggta gtcgtcgcac acggcaaatt ggatgtcgtg cacttcgata
    34081 ttcgacctgc cggcattgcc gccgaggtaa aacatatgga gcttgggcat atcgtattcc
    34141 ttatggtttg cgcgtgttca gacggccttt ttactttcca gatattccgc cagcccgcgt
    34201 tcgcgcaaga tacagctcgg gcattcgcgg cagccgccga cgatgccgtt atagcaggtg
    34261 tgggtttgct cgcggatata gtccagcgcg cccatttcgt ccgccaacgc ccaagtttgc
    34321 gccttggtca gatacatcag cggcgtgtgg atttggaaat catagtccat cgccagatta
    34381 agggtaacgt tcatggattt gacgaacacg tcgcggcagt caggatagcc ggagaagtcg
    34441 gtttcgcaca cgcccgcgat gatgtgccgt atcccctgcc ctttggcgta aatcgcggca
    34501 tagagcagga aaagcgcgtt gcggccgtct acaaaggtat tcggaacgcc gttttcggca
    34561 gtttcgatgg cggcggtgtc gtccatcagg gcattgtgcg taatctgccg catcaggctc
    34621 aagtcgagta cggtttgttt gacgcccaaa tcctgcgcaa tccagctggc acgttccagc
    34681 tcgacggcat ggcgttgccc gtattggaaa gtaatggctt ggacgttttc gcgcccgtag
    34741 atttggattg cctgaatcag gcaggtggtc gaatcctgac cgcccgaaaa gatgaccaag
    34801 gctttttggt ttgacatatc gaatcctagt tttggtaacg cgggaggttt gcgaaccgcg
    34861 aagttggaca gtaaagacgc ggattataca ggcaaacgcg ttcagacggc atctgaacgc
    34921 catgccgtct gaacacgcgg cttatgccga cttgacggcg agttccgcca agcgtttgct
    34981 ttgtgcgaag gcgatattgt tttcttcggc ggtcaggacg ggagccgctg tcgggaatat
    35041 cttttttgac ggcttcgcag acggcggaga ctttggggac ggtgcgcaat acggcttcgc
    35101 aaccttcaac gctgtcgatg ccgcgagcga ttcggagtgc gggattgcgg gtgctgccgt
    35161 tttggaaata gtagaggacg aggattttga gggggatttg ggttcatggg cggattccgt
    35221 taaaatggtt cggtcttcag acggcataac agttgttccg atgccgtctg aaacagccgc
    35281 agttcaggca tttgttcctg acggcaaaat tggctataac atcgggcatc aatgcccaac
    35341 ttactgaccg ggcaagaaat tatgaccttt ttacaacgtt tgcaaggttt ggcagacaat
    35401 aaaatctgtg cgtttgcatg gttcgtcgtc cgccgctttg atgaagaacg cgtaccgcag
    35461 gcggcggcaa gcatgacgtt tacgacgctg ctggcactcg tccccgtgct gaccgtgatg
    35521 gtggcggtcg cttcgatttt ccccgtgttc gaccgctggt cggattcgtt cgtctccttc
    35581 gtcaaccaaa ccattgtgcc gcagggcgcg gacatggtgt tcgactatat caatgcgttc
    35641 cgcgagcagg cgaaccggct gacggcaatc ggcagcgtga tgctggtcgt tacctcgctg
    35701 atgctgattc ggacgataga caatacgttc aaccgcatct ggcgggtcaa ttcccagcgt
    35761 ccgtggatga tgcagtttct cgtctattgg gctttactga cgttcgggcc gctgtctttg
    35821 ggcgtgggca tttcctttat ggtcggctcg gtacaggatg ccgcgcttgc ctcaggtgcg
    35881 ccgcagtggt cgggcgcgtt gcgaacggcg gcgacgctga ccttcatgac gcttttgctg
    35941 tgggggctgt accgcttcgt gccaaaccgc ttcgttcccg cgcggcaggc gtttgtcggg
    36001 gctttggcaa cagcgttttg tctggaaacc gcgcgctccc tcttcacttg gtatatgggc
    36061 aatttcgacg gctaccgctc gatttacggc gcgtttgccg ccgtgccgtt ttttctgttg
    36121 tggctgaacc tgttgtggac gctggtcttg ggcggcgcgg tgctgacttc ttcactctcc
    36181 tactggcagg gagaagcgtt ccgcaggggc ttcgattcgc gcggacggtt tgacgacgtg
    36241 ttgaaaatcc tgctgcttct ggatgcggcg caaaaagaag gcaaagcctt gcctgttcag
    36301 gagttcagac ggcatatcaa tatgggctac gacgagttgg gcgagctttt ggaaaagctg
    36361 gcgcggtacg gctatatcta ttccggcaga cagggttggg tgttgaaaac gggggcggat
    36421 tcgattgagt tgaacgaact cttcaagctc ttcgtttacc gtccgttgcc tgtggaaagg
    36481 gatcatgtga accaagctgt cgatgcagta atgatgccgt gtttgcagac tttgaacatg
    36541 acgctggcag agtttgacgc tcaggcgaaa aaacagcagc aatcttgagt atttttgaaa
    36601 ctgtattttt atcctaaaga cattttccta tctgctgatt ccaccgtttc ctttctgtgt
    36661 gccgccataa tttttatgat agacaagcca ttcaagtttg cattgactgc tttttcattg
    36721 gcaatatcca aatggctggt cgctgcatct aaaaatagga ttttcggttc gcaatataag
    36781 gcccgcgcca atacgatgcg ttgtttttgt ccgcctgaca gtgcgctgcc catatcgccg
    36841 atcaaggttt catagcccat aggcatggcg gagatttctt cgtgaatcat agcaaggcag
    36901 gcgcaatgtt cgatttttcc tctatccggt tccgtatcga aaaaacaagg ggctgtacta
    36961 gattagccct aaatcccaca ccaatcccgc aggattttaa gctgttgaga cggtgtgccg
    37021 aagttaaatc gaaattcgca ttctttcaag aacagcggga aagatttacg atcgattccg
    37081 ttgtattttc gcaagacgcg tttagtctag agtctgtata ttacatttat ttttagggtc
    37141 tgctagccaa tttcttgttc ccttcattat tttatcttct gaaagaaaat tatttttttc
    37201 catgctatta atattaatga tatgattttt atttaaaata atttttccat ataaaatttc
    37261 cggtttgtaa tagcaagagt ctgaagcaaa tccttgcccg tcaatataag aaaaataaat
    37321 gttatcatcc tccttataat attctaaaac caatccggat attattttat caaaattccc
    37381 ttcatttcca tctttcatga ctaaaatata attagcaggc caacctttat cgtaaacata
    37441 ttcaaactga tagttcccga aactctcgat atccgaacta aaaaagaaga aaagcaaatt
    37501 aatagccaat acaaaaacaa ataagaacaa aaatagcaaa attttcaact tagttaacaa
    37561 tatttacctc tcctttaaat tcaatcctga aaggtacccc ttaccctggg ctaccaatta
    37621 tagtttccat atttctaaat attgttttta cattactttt ttctcccccc aaaggaatgc
    37681 attttaaaat catgcttttc aggtgctaat cgatacttac cattaacatc tttaatcaca
    37741 gatatatttc catgtatagc ccaacgtgaa aaatctgagt attatataca gttatacctt
    37801 actgaaacaa agctagctgt acatatctgt accatatttt tactacaaac aaaaaatatt
    37861 tataattatt aaggataaga taaaaacccc caacgctaga ttaattctta ataatttcaa
    37921 ataaaaaata tttgagattt tttcttttcc aaataatata ttgaaatatt taaagttaag
    37981 gtagcttgat ttaagtagaa aatcaaaaaa accattaatt aatttttctt ctttaaatat
    38041 tttatatgat attataaaat taaataacat tgcgaaaaca tatgaaatca tcaaaatacc
    38101 aaatatatag tgggaatgca ttattattta gctccttctt ttggctctga caaattgaca
    38161 gtccattgat atccaatgct tgcatttgca attgctgacg cacctgcccc tgttttccct
    38221 gttagtatca atggttcttt tcttgcaaga tgtgcacctg cacaaccacc taatactaaa
    38281 ccaccaccta ttgtaaatcc ttcctgttgt atctctaatg ataacatttt cttcttgtgc
    38341 cgcccttaat acagattgtg gaattggatt gacctgagca ggaacttcta aaataagttt
    38401 tccttcaagc tgtcttcctc ttagcgaagc aggtgtactt ggaacattgg aaatgacaaa
    38461 gtgagtgcgc aaagccacct gaaggcgatg tgtttgaa
//
LOCUS       DAWHXS010000006        35520 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWHXS010000006 DAWHXS010000000
VERSION     DAWHXS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.006"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(353..655)
                     /locus_tag="WIE22_000226"
     CDS             complement(353..655)
                     /locus_tag="WIE22_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ4126374.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(627..914)
                     /locus_tag="WIE22_000227"
     CDS             complement(627..914)
                     /locus_tag="WIE22_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ4126375.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            994..1599
                     /locus_tag="WIE22_000228"
     CDS             994..1599
                     /locus_tag="WIE22_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126376.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            1578..1874
                     /locus_tag="WIE22_000229"
     CDS             1578..1874
                     /locus_tag="WIE22_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ4126377.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            1871..2080
                     /locus_tag="WIE22_000230"
     CDS             1871..2080
                     /locus_tag="WIE22_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126378.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(2190..3332)
                     /locus_tag="WIE22_000231"
     CDS             complement(2190..3332)
                     /locus_tag="WIE22_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ4126379.1"
                     /translation="MQKWQTELYSTPLWLCLTPKSTVKVLLLITVMVTLLLTEVRLNV
                     LSTFMSKGLYDSMQDLNASAFWMFAAMNAGVVLIRAFNNVVNDFLDQGLAIKWSEQLN
                     EVLTTRWLADKNYYRLQMRRHAPDNIDQRIQQDAQDFIASTIEFVRGMVNSVVTSLEF
                     AVVLWGLAGILTVFGFDIPRGIVWLVFIFVILATFIAMWIGNPLIRYNYENEKLNGDY
                     RYSLIRVRDHAESVAFYNGEQHEHGRLSDRFKAIIRNRWRIARQSVCLSGFNDMFTNG
                     IKLFPIILQAPRLFAGQIKIGDIQQTVQAFARLQNALSFFRMFYNKFTAYRARLERLY
                     GFLLSTEEQHSARQPEISEVSDGIALECKAAAAFARIGGSFCTAEA"
     gene            complement(3775..4422)
                     /locus_tag="WIE22_000232"
     CDS             complement(3775..4422)
                     /locus_tag="WIE22_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ4126380.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(4419..4787)
                     /locus_tag="WIE22_000233"
     CDS             complement(4419..4787)
                     /locus_tag="WIE22_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ4126381.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(4797..5468)
                     /locus_tag="WIE22_000234"
     CDS             complement(4797..5468)
                     /locus_tag="WIE22_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ4126382.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(5633..6352)
                     /locus_tag="WIE22_000235"
     CDS             complement(5633..6352)
                     /locus_tag="WIE22_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ4126383.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(6436..7749)
                     /locus_tag="WIE22_000236"
     CDS             complement(6436..7749)
                     /locus_tag="WIE22_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ4126384.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARYADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKGKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(8147..9019)
                     /gene="hpnD"
                     /locus_tag="WIE22_000237"
     CDS             complement(8147..9019)
                     /gene="hpnD"
                     /locus_tag="WIE22_000237"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ4126385.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYCE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            9190..11052
                     /gene="hscA"
                     /locus_tag="WIE22_000238"
     CDS             9190..11052
                     /gene="hscA"
                     /locus_tag="WIE22_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ4126386.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGGNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMPIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLEAEELQQI
                     RQGIADLQGRVKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            11128..11775
                     /locus_tag="WIE22_000239"
     CDS             11128..11775
                     /locus_tag="WIE22_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ4126387.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRTELFAVDENLVKRLFLSLRGEIVFPKNETA
                     ESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRL
                     FKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR"
     gene            11811..12572
                     /locus_tag="WIE22_000240"
     CDS             11811..12572
                     /locus_tag="WIE22_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ4126388.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGASPASTTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            12628..12969
                     /gene="fdx"
                     /locus_tag="WIE22_000241"
     CDS             12628..12969
                     /gene="fdx"
                     /locus_tag="WIE22_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ4126389.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(13256..13843)
                     /locus_tag="WIE22_000242"
     CDS             complement(13256..13843)
                     /locus_tag="WIE22_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ4126390.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            14056..14253
                     /gene="iscX"
                     /locus_tag="WIE22_000243"
     CDS             14056..14253
                     /gene="iscX"
                     /locus_tag="WIE22_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ4126391.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(14321..14722)
                     /locus_tag="WIE22_000244"
     CDS             complement(14321..14722)
                     /locus_tag="WIE22_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ4126392.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            14933..15892
                     /locus_tag="WIE22_000245"
     CDS             14933..15892
                     /locus_tag="WIE22_000245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ4126393.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            15960..17270
                     /gene="tilS"
                     /locus_tag="WIE22_000246"
     CDS             15960..17270
                     /gene="tilS"
                     /locus_tag="WIE22_000246"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ4126394.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            17321..18106
                     /locus_tag="WIE22_000247"
     CDS             17321..18106
                     /locus_tag="WIE22_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ4126395.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            18116..18313
                     /locus_tag="WIE22_000248"
     CDS             18116..18313
                     /locus_tag="WIE22_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126396.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            18583..18927
                     /locus_tag="WIE22_000249"
     CDS             18583..18927
                     /locus_tag="WIE22_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126397.1"
                     /translation="MRIFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLSCYY
                     DKVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKELDEV
                     LLKYIPFFEEER"
     gene            18939..19244
                     /locus_tag="WIE22_000250"
     CDS             18939..19244
                     /locus_tag="WIE22_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126398.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            19306..20682
                     /gene="mpl"
                     /locus_tag="WIE22_000251"
     CDS             19306..20682
                     /gene="mpl"
                     /locus_tag="WIE22_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ4126399.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            20889..21941
                     /gene="bioB"
                     /locus_tag="WIE22_000252"
     CDS             20889..21941
                     /gene="bioB"
                     /locus_tag="WIE22_000252"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ4126400.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(22020..22654)
                     /locus_tag="WIE22_000253"
                     /pseudo
     CDS             complement(22020..22654)
                     /locus_tag="WIE22_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            22977..23237
                     /locus_tag="WIE22_000254"
     CDS             22977..23237
                     /locus_tag="WIE22_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ4126401.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(23300..25159)
                     /gene="ilvD"
                     /locus_tag="WIE22_000255"
     CDS             complement(23300..25159)
                     /gene="ilvD"
                     /locus_tag="WIE22_000255"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ4126402.1"
                     /translation="MPDYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPHSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDIAQIER"
     gene            complement(25332..27101)
                     /gene="cysI"
                     /locus_tag="WIE22_000256"
     CDS             complement(25332..27101)
                     /gene="cysI"
                     /locus_tag="WIE22_000256"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ4126403.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSYYRSIRLTNRQTFQFHGVPKTKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESALHQQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGGGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDDLDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(27128..28942)
                     /locus_tag="WIE22_000257"
     CDS             complement(27128..28942)
                     /locus_tag="WIE22_000257"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ4126404.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAASANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLSYQ
                     AGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(28939..30225)
                     /locus_tag="WIE22_000258"
     CDS             complement(28939..30225)
                     /locus_tag="WIE22_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ4126405.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDESTYGTVAAGMIL"
     gene            complement(30264..31187)
                     /gene="cysD"
                     /locus_tag="WIE22_000259"
     CDS             complement(30264..31187)
                     /gene="cysD"
                     /locus_tag="WIE22_000259"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ4126406.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(31225..31965)
                     /locus_tag="WIE22_000260"
     CDS             complement(31225..31965)
                     /locus_tag="WIE22_000260"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ4126407.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRDIGKYNPIFDWSEHDVWAYILTNNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(31976..33427)
                     /gene="cobA"
                     /locus_tag="WIE22_000261"
     CDS             complement(31976..33427)
                     /gene="cobA"
                     /locus_tag="WIE22_000261"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ4126408.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQTYPALGGLNAGQRAA"
     gene            complement(33877..34533)
                     /locus_tag="WIE22_000262"
     CDS             complement(33877..34533)
                     /locus_tag="WIE22_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ4126409.1"
                     /translation="MKNKSKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQIGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFNLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            34598..35488
                     /locus_tag="WIE22_000263"
     CDS             34598..35488
                     /locus_tag="WIE22_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ4126410.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWVVGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT         7812 a         9320 c        10202 g         8186 t
ORIGIN      
        1 aagcgggaat ccagaccccg acgcaacagg aatctatcgg aaaaaacaga aacccccgcc
       61 gccgtcattc ccgcgcaggc gggaatccag accccaaacg cggcaggaat ctatcggaaa
      121 cggctgaaac cgaacggact ggattcccgc ctgcgcggga atgacggaaa gtggcgttcg
      181 ggattcccgc cttcgcggga atgacggaaa gtggcgggaa taacgaaagg cgggaatgac
      241 cgcgcaaaaa gccgctgccc ccttcggacg gcaccggcaa caaaaaaccg cacggccgaa
      301 accgcgcggg aaaggatagt cgggcgcgcc cgataagcag cggccgcccc cgttatttca
      361 attgggcgac atattggcgc aaaacctcgt tgatgcgcgt ctgccagccc ttgccgccgg
      421 cgcggaattt ttcgaccaca tcggcggaca ggcgtatggt aacgagttgt ttcggggttt
      481 tgcctgtgtt tcgtttttgc attacgccct tttcttccaa ttgtttttga tgggaaaaaa
      541 gcacctgtgc caagtcttcg ggcagggctt cggcaatggg gcgggcaagc gcaaaatctt
      601 cggcggcaag ttcccgcact tcgccgtcag cgtttgttaa ggattgacgt tgcatatttt
      661 ttaacctctc ttttattcgc tttgcgaaaa ctgatgacac ggatgccgtc ttttatcggc
      721 gtaaaacaga caatgtgcag gcgttgcgta tcgcctagat aagcagcggc aacataacgc
      781 ggttcggggt aatcaaagca gacatcgggc acaataacgg ccgttgtcca gcgtatttgc
      841 ccgactgatt caaagggcaa attccgctct tcgatattgc gttgattttt ttcggagtca
      901 aattcaatct tcattgcagc ttgcagcgta ttttgtcgtt acattataag cggcaaaaaa
      961 ccaaaatgta aatacaaaaa aggaaacccc gaaatgacca tctatttcaa aaacggcttt
     1021 tacgacgaca ccttgggcag catccccgaa ggcgcggttg ccgtccgcgc cgaagaatac
     1081 gccgcccttt tggcaggaca ggcgcagggc gggcagattg ccgcagattc cgacggccgc
     1141 cccgttttaa ccccgccgcg cccgtccgaa taccacgaat gggacggcaa gaaatgggaa
     1201 atcggcgaag ccgctgccgc cgcccgtttc gccgaacaaa aaaccgccac ggcattccgc
     1261 ctcgcggcaa aggcggacga actcaaaaac agcctcttgg cgggctatcc ccaagtggaa
     1321 atcgacagct tttacaggca ggaaaaagaa gccctcgcgc ggcaggcgga caacaacgcc
     1381 ccgaccccga tgctggcgca aatcgccgcc gcaaggggcg tggaattgga cgttttgatt
     1441 gaaaaagttg tcgaaaaatc cgcccgcctg gccgttgccg ccggcgcgat tatcggaaag
     1501 cggcagcagc tcgaagacaa attgaacacc atcgaaaccg cgccaggatt ggacgcgctg
     1561 gaaaaggaaa tcgaagaatg gacgctaaac atcggctgaa aaaatacgtt taccacctgt
     1621 tggtagccat cgaccaactg ttcaacgccc taaccggcgg cgcggcggac gaaaccctct
     1681 caagccgcac ctatcgcggc gcgcggctcg cccaaaagcc caaaacccgc tggaaggttt
     1741 tatacaccct gatcaacggc gtatttttcg accgccaaca ctgccggcag gcgtatatca
     1801 gcgaactgaa aggcaggcag cacgacgcgc ggttcaacca aagccgcgcc gccggggaaa
     1861 aggggacgcg atgaactact tcggcatggt agagtttctg cgcctgatgg caatggtgcg
     1921 gccgccattt gtcttcttct ccggcacccg cagcgaactg cctacttatc ttgacctcgt
     1981 ggcagaactg cgcctgaccg gatgggagcg ttttgcaggc agccaaacct tgactgtgag
     2041 cagcacatca acagcaattc aagctacgac gaccacctga tttataagtt ctgaccgcaa
     2101 gtagcgtact acttttaaag gcataagata atccccgttt aacagaccat taaacgggga
     2161 taaatttgtg caaaagctaa tacaatttcc tacgcttcgg cggtgcaaaa gctgccgcca
     2221 attcgtgcaa aagctgccgc cgccttacat tccagtgcaa tgccgtctga aacttcgcta
     2281 atctcgggtt gccgcgcgct gtgttgttct tcggtactca gcaaaaagcc gtacagacgc
     2341 tccaatcggg cgcggtaggc ggtgaatttg ttgtagaaca tccggaaaaa agatagcgcg
     2401 ttttgcagtc gcgcaaaggc ttggacggtc tgctggatgt cgccgatttt gatttgtccg
     2461 gcaaacaggc gcggcgcttg caaaataatg gggaagagtt tgatgccgtt ggtgaacata
     2521 tcgttaaagc cgcttaagca gacgctttgc cgcgcgatgc gccaacggtt gcggataatg
     2581 gctttaaagc ggtcggaaag ccggccgtgt tcgtgttgtt cgccgttgta aaacgctacg
     2641 ctttcggcgt ggtcgcgtac gcggatgagg gaataacggt agtcgccgtt gagtttttcg
     2701 ttttcatagt tgtaacgaat caaggggttg cctatccaca tcgcgataaa ggtcgccaaa
     2761 atcacaaaaa taaagacgag ccaaacaatg ccgcgcggaa tgtcgaagcc gaacacggtc
     2821 aggatgccag ccaagcccca caaaacaacg gcaaattcca aagaggtaac gaccgaattg
     2881 accatgccgc gtacaaattc gatggtcgaa gcgatgaaat cctgcgcgtc ctgttggata
     2941 cgttggtcga tgttgtccgg cgcgtggcgg cgcatttgca ggcggtagta gtttttgtcg
     3001 gcaagccagc gcgtcgtcag cacttcattg agctgctccg accatttaat cgccaagcct
     3061 tgatcgagga agtcgttgac gacattgtta aacgcccgta tcagcaccac gcccgcgttc
     3121 atcgcggcaa acatccaaaa cgcggaggcg ttcaaatcct gcatcgagtc gtaaagtcct
     3181 ttggacataa aggtactcaa cacattcagc cgtacttcgg tcaacagcag cgtaaccatt
     3241 accgtaatca gcagcaagac tttgaccgtg cttttcggtg tcaggcaaag ccaaagcggt
     3301 gtggaataaa gctcggtttg ccatttctgc atgggaaatt tcttacggta tcaatgccgt
     3361 ctgaaaaaga cgggtacagt tgattttttg atgaagtttg gggaagtttt gccggtcagg
     3421 gtacattgcg tgttaattta tagtggatta aatttaaacc agtacagcgt tgcctcgcct
     3481 tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgagtcgg ttccgtacta
     3541 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tataccatac
     3601 aaccacgccg gaattaagtt taaatttgaa taaaaggttc gggttctgca aaatacagaa
     3661 cccgaacctt gttcggatat tgaaaccggc cgcccgattt tgggcggtgc ggcttgcaag
     3721 tatcaagatt cgcatatgcc gtctgaagct cggagaggtt cagacggcat atgcttattt
     3781 gggctgctct tcgacgaatc tcggaccttt caagatgccg ttgtgagaat agggcgacag
     3841 caggttgtat gcggcggttt tggaaacctg ataaccgcgg tcggtcaggc tgttggcaat
     3901 ctgattgacc actgcgctga ccaaagcccc caacaggccg ctgttgctgt tgttgctgcc
     3961 ttcgcggatg ctggccgaac ccgaccacaa ctcttttccg ttgcgggaat cgaccagccg
     4021 tgctttggcg gatacggtcg tcacgctgtc taaaatttga tatgaagtgc cgtattcggt
     4081 aaccgtaatg tacaaaaccg catcattgcc gaaaatctga tgcagttttt ccggccggac
     4141 ggcgtgaata tcggcggcat tggtcaagcc gttttgtttg aaggtttcct ccacgactgc
     4201 ggcggggaag acgtaatagc cggcttcgga aagcggcgcg gcggtcgaag ccagtacacc
     4261 ccatgttccg ttgacatcgg gcgattcgtt cagcggcgga accaccaaaa ttgaagccgg
     4321 tttgctttcc ttgaatgacg tgtagtcgaa atcgggcgct ttttgaactt ggcaggcaga
     4381 cagcgccaac acggcggcaa gccctaaaat caaaggtttc atcgcttgcc tcctttacct
     4441 gttttcatca ggaagtccat aaatacgccc gattcgggaa acagcctttt ctcttcttca
     4501 aactggcgga acgcgccctc tttgtctccc gaacgggaaa gcagcagtcc cagatgggcg
     4561 tgcgcacccg gggcggcatt catttttttg ttgccggctt ccacaaagta tttttccatc
     4621 ttttcggtct gcttgcccaa cgaagtgtcg tcgtttttca aaccttcata gacggtatcg
     4681 ggatagccgc cgtaataata cagggatttt tgcccgttgc cgccgcaggc ggtcagagcc
     4741 aagaccgccg cacacagcga caaacggctc aaggttttcg gattcatcat ttctccttaa
     4801 cggttgggtt gccatgcgcc gttgtcaaca gcctgaacca ggctgttgac ggcttcgcgg
     4861 attgccaagt ctaaaacttt gccgttcaaa gtcgcatcgt agccggaagt gccgccgaaa
     4921 ccgatgattt cacggttgga aagtgcgtat tcgcccgcgc cctgtgcgga atagacgatt
     4981 tcggaagtat tgacgttgac gatattcaga gccacttttg cataggcgat ttgcgatttg
     5041 ccgcgaccca aaatgccgaa gagctgatga tcgccgacat ctctgcgtcc gaattcggtt
     5101 acatcgccgg taacgacata atctgcgcct ttcaggttat gcgctttgcc ggaaatgccg
     5161 gattcctgtt ttaatgcgtt caaattggtg cggttcagta cgttgaagcg gttggtctgt
     5221 tgcaggtgcg ttaccagaat ggtttttgcc tggctgccca aacggtcttc cccgtcggag
     5281 aaaatgcctt tttggaagct ggagcggttg tcgaatgttc cgacggaaat cggggtacga
     5341 acaccgtgat attgcgtatt gtaggaggcg actttctcta cctcgagact gcgtgaggat
     5401 tcggtcgcac agccggtcag tgaaacggca gcggcggcaa ggacaacggc ggtggaaacg
     5461 gttttcataa aatttaccct aaggtcaagt taaggaaata acgggttatc attattgtcc
     5521 ttatgtaaat ttaagtcaag gtgtttgtct gtgcgggacg gatgcgcgcg gaaggtacgg
     5581 atatttttca aacggaatcg cggcgcgggc gggatgcctg ccttttccgg atttagaggt
     5641 aaacgatttc gccgctccgc cctttgcttt cggtacttgc ccaccagaca aatgcgggca
     5701 gcacgtcccc gtagcttttg cgttcgcttt tggcttcgcc cggatgggat ttgatgcgtt
     5761 gcggggaatt gatggggccg gggacgagga cgttggcgcg caggttgccg aagcgttccc
     5821 attcgtcggc ggcgactttg cacaggtagt tcaacgcggc tttggacgcg ccgaagccgc
     5881 cccagtaggc tttgggtgtt tcgccgtggc tttcgccgac gaagatgacg gacgcgtcgg
     5941 gcgactgctt cagcagcggg aacagggcgc gggtcagccc cataggtgcg acggtgttga
     6001 tgcggtattg gttgacccat tcggcgacgg tttggaaatc cagcggcgag agggcgtaaa
     6061 aatagccggc gcagtggacg atgccgtcca gtttgccttg cgtggcttcg gcaatggtgg
     6121 cggcgaactg attgaattct ttttcttccg cactcatcag gtcgaaacgg atggcgaagg
     6181 gttcggggtg tccggcttcg acaatcgcgt catagacttt ttccagtttt ttctgatggc
     6241 gtgccaccag aatcacggtt gcgcctgccg ccgcataggc tttggcgacc tgttcgccca
     6301 gaccttgcga tgcgccggta attaagatgg ttttgtcgga cagtgtcgcc atactttttc
     6361 ctttttggtg gtcggtcggg ggtattttag cgttttgccg caccttgtaa agcgtcccga
     6421 taccggccgg cgttttcaga cggcatcgct caggctttgc aggcaggctt ccgccgacgc
     6481 gaaacctgat tgtacggcgg cttcgagcgt ggcggggtag tctgggtgga ggtagtcgcc
     6541 ggcggggaag atgcggtgcc ggtgcaacca cgacaaatcc ggcggcgggg catcggctgc
     6601 ggttgtggcg cgtttttcgg tgatgacgcg cacggcttcg ggttcgccca aatgcggaag
     6661 gatgcgtttg aggtcggcgt gaactttgtc cgcccacgct cggtttgtaa acgcgccgac
     6721 gcggtcggaa acgctgatga cggcggacac ttcgttttca ggcagtccga gcctgccccg
     6781 gcaaagcaac cattgtgccg tgccgtcggc aaggccggtc agcggggcgg gcaggcggac
     6841 gggttcggcg tagcgcagat agacggtggt gatggcgtga tagcgaaggt tttgatatgc
     6901 cgtctgaacg tgttcgggcg tgccttcggg caggagcgcg gcggcgtggt agggcgcggt
     6961 ggcggggacg gcggcatcga aagcttcgcc gttgacgagc actttcccgt ccgggagggg
     7021 gttcagacgg catacgcgcg tttcgaggcg gatgtccgcg ccgagccgtt gaagctccgc
     7081 caaggcaggt tcggcgacga ttgcgcccaa atcctgcttg ggtaggagat agtcgctgcc
     7141 ggattttttc gtcagcacgc cgtcggacaa aacgttgcac aacacgcgca ggcttgcggt
     7201 ttccaaaggc gtgttgagcg caccccacac cagcggctgc caaaactgca tcacggcggc
     7261 acgcggcacg ttccgctgtt tcagccattg tgcaactgtc gtgtcgggct gtccgaggcg
     7321 tgcggacttc tgcaaatcgg acatatcggc aagcagtttg cctttgaatg cggacggtac
     7381 gcgccgggca agcagcacgc cgcccaaaat atgcagcggt gcgggcaggg ggagggcgcg
     7441 gaactgtaaa ccgccgtgca tatgccagtg cagcggtacg cgcaaaaagg cggcacgggg
     7501 gtctgaaccg atggttttca tcaggcgcaa cacgccccgg tatgcgccga gcaaaatgtg
     7561 ctgcccgttg tccaaaaaac cgaaaccgtc ggtatttccg gccagtgcgc gcgccctgcc
     7621 gcccgcctgc cggccggctt caaacagggt aacgtcggcg taccgcgcca aggtgacggc
     7681 ggcggacagt cctgcccagc ctgcgccgat gacggcgatt ttcgggcgcg gatgcggcgt
     7741 gttcatcatt tattcctcca atggttttgc agccgtatct atttccgttt ctgaaaatga
     7801 cggtagaaaa ggatacaggc taaaaataaa ggcagcagaa agcgcgcata gggatatcac
     7861 ggatggtctg catgccagta tccgtaccgt ccctgtggaa cggtatcata gcggtaattg
     7921 tcggggaaaa agttgatcca aaccgttact gccggccata aggctatatc cctgactgct
     7981 ttcatttgct tctgctcctg tttaaattcc cggcaattcg atttcaaagc gcgaacgcca
     8041 acgggattgc gcggttacga tgcagacttt cagacgatgg ttgaaacccc gtccggcggg
     8101 gctgtgggaa tcgggtttgc ctgacgggcg gcgggcggta tgcgccttat gcccgttccg
     8161 gcgtgccggg gcgcggtttg aatccgaata accaggtttt cagggcaatg cgttttttgc
     8221 gcggcgaagg gagggcgatt ttgtatttga ggacgttttg tgcgccgtcc cggtcgattt
     8281 cgttcaatag ctcgtaataa accgccgcca taaccagtcc gactttttgg gcttttttat
     8341 cggcagccgg cagcagcgat acggcttcgc agtaggtttc acgggcgcgt ttgatttgga
     8401 acgccatcaa ttcggcaaaa ttgcccgtcg ggctgcattg caaaatcacg cttgcgggta
     8461 cgtcaaaccg ccgcatttcc tccatcggca ggtaaatccg tcccctgcgc gcgtcttcgc
     8521 cgacatcgcg gatgatgttg gtcagttgca gcgcaagtcc catcttgtcg gcgtattcca
     8581 gcgtttggtc gtctgaaaac cccaaaatcc gcgcaatcag gcagccgacc acgcctgcga
     8641 cgcggtggca atacagtttc aattcttcaa aactgccgta acgggcttga accaaatcca
     8701 tctgcatccc gtcgattaag gcttccagtt catatttcgg cagcttgaag gtttccttaa
     8761 cttgccgcaa ggcctgattg acggggtgtt ccggcatcgc gccgccgaat accttgtcca
     8821 aatcgccgcg ccaccagttc aatgtcgcct gtgcaacatc ggggttggaa cattcgtcaa
     8881 ccacatcgtc caattcgcgg caaaaagcat ataaaaccgt taccgcatcc cgtttttcct
     8941 gagtcaggaa acggaagccc gacaaaaaac tggagcggct ttcttctgct ttttggcggc
     9001 aatagtcgag tcctttcacg atttatattc ctaatgatgg gcgggaaagg cggattttat
     9061 cggcatttgg cggtagaggg caatttcggc ggcacgacct aatccttagc ggtttgctca
     9121 actatcggcg caaattctgt taaaatgccg ctttccttcc tttacacacc gcaccgacag
     9181 gcagaattta tggctctttt gcagatttca gaaccgggta tgtccgccgc cccgcaccgg
     9241 caccgtttgg cggcaggcat cgatttgggt acgaccaaca gcttggtcgc caccgtccgc
     9301 agcggcagtg ccgcctgcct gcccgatgcc gaagggcgcg ttaccctgcc ttccgtcgtc
     9361 cgctatctgg aaaacggcgg cattgaagtc ggcaaaaccg ccctgtccgc ccaaaaaacc
     9421 gacccgctga acaccgtcag ctccgccaaa cgccttatcg ggcggacgct tgccgatctg
     9481 catcaaaata cgcactacct gccttaccgt ttcggcggca atcaacgcgt tatcgaactg
     9541 catacgcggc agggggtgaa aacgcctgtc gaagtgtcgg cggaaatcct caaaaccctt
     9601 aaatcgcgcg ccgaagaaac cttgggcggc gatttggtcg gcgtggtgat taccgtcccc
     9661 gcctatttcg acgatgccca acgccaggcc accaaagatg ccgcgcgtct ggcgggtttg
     9721 aacgtattgc gcctgctcaa cgaacccacc gccgccgcaa tcgcctacgg gctggacaac
     9781 gcctcggaag gcacgtttgt cgtgtacgac ttagggggcg gcacattcga cgtatccgta
     9841 ttgcaactga ccaaaggact gtttgaagtc aaagccaccg gcggcaacag cgcgttgggc
     9901 ggcgacgatt tcgaccaccg cctgttctgc cgcctgctcg aacaaaacgg actctcccaa
     9961 ctcaacgaac aagacagcca actcctgctc tcgctcgtcc gcgccgccaa agaacaatta
    10021 accacgcaaa ccgaagcgcg cattcaggcg acgctttcag acggcatgcc aatcgacaca
    10081 agcatcagtc gcgccgagtt ccacaacctg acgcagcatt tggtgatgaa aacgctcgaa
    10141 ccggtcacac aggcgttgaa agatgccggt gtcggtaaaa acgaagtcaa aggcgtgatt
    10201 atggtcggcg gttcgacccg tatgctgcac gtccaacagg cagtcgccac cttcttcgga
    10261 caaaccccgc tgaacaacct caatcccgac gaagtcgtcg cgctcggcgc cgccatacag
    10321 gcaaacgtcc tcgcaggcaa caaaaccgac ggcgaatggc tgctgctgga cgttacacct
    10381 ttgtcgctcg gtttggaaac ctacggcggc ttggcggaaa aaatcatccc gcgcaattcc
    10441 accatcccca ccgcgcgcgc gcaggacttt actaccttca aagacggtca gaccgcgatg
    10501 acgatacacg tcgtacaagg cgagcgcgag ctcgtttccg actgccgcag ccttgccaaa
    10561 ttcaccctgc gcggcattcc gcctatggcg gcgggtgcgg cgcgtatccg cgtaaccttc
    10621 caaatcgatg cggacggtct gctgtccgtt tccgcccaag aacaaagcac cggcgtacag
    10681 gcgcaaatcg aagtcaaacc ttcctacggc ttggacgacg acaccatcac ccaaatgctc
    10741 aaagacagca tgagcaacgc cgccgaagac atggcggcac gtgcccgagc cgaagccgtg
    10801 gtcgaagccg aaagcctgac cgatgccgtc aacgccgccc tcgagttgga cagcgatttg
    10861 ctggaagccg aagaattgca acagattcgg caaggcatcg ccgatttgca aggccgtgtg
    10921 aaagacggaa aagccgaaga catccgtgcc gccgccgcca aactcggcag tattaccgac
    10981 aatttcgccg ccaaacgcat gaaccgcaac atccaacgcg cgttgactgg tcaaagcgtt
    11041 gataatattt gacggcaaat ggtttcagac ggcatagata taacctgata ccgtctgaag
    11101 gctcgaaaac acttgaaaaa catcgatatg gaaaagtcag gcattgtcta tctgatgaaa
    11161 accgtcatca agggcgtgta taaaatcggc atttcggacg taagcaattt tgaaggcaga
    11221 atgcgccatt tggaaaacaa cggctatgcg aacgttgccg gattggaacg catcctcgcc
    11281 gtcaaaaccg acaattacaa agaaaaagaa aacctgctcc atgaaatttt tagtaaaagc
    11341 aggaccgaat tgttcgccgt ggacgagaat cttgtaaaac gtttgttttt atcgcttcgc
    11401 ggcgaaatcg tgttcccgaa aaacgaaacg gcggaatcgg aatttgaaaa aagcgtccac
    11461 gaacgcaggc aggaagggaa tgccggatcc ggtcgcaaac aactgcttga tttggtacgg
    11521 cgcggacacc gggaataccc ttacgcgctg ccccggcttt tggcgggcgc ggcatcctac
    11581 aagccgaaaa aatcgaaaat ccgccttttt aaagaagcat atttacgcaa aagcggtacg
    11641 aggctgaccg acgaaattgc agacggcatc catatttaca cctgtttttc gcgggcggat
    11701 ttggaaaaag cctattccga atatttggaa cttttcaaat ccgaatcgga tgccgaaggc
    11761 agaaaaccac ggtaaggtgc aaacagatac cgtacacgtt gaggagcagt atgatgggcg
    11821 attccgtcat ttattatgta gaacaggcag acgaaccggt aaaccgtgcc ggcgaacgcg
    11881 cccgtaaaac attcaaatac ttttggcgcg agcttttttg ggaacgccgc cgcatcattc
    11941 ccgccttaga ttttgccatg gtcaaagccc cttttttcca agacggcgaa gacggcgaaa
    12001 tttgcgaaca tatgtggatc gacgatattt attttgacgg tctttacatt tatggcgtgc
    12061 tgaacaatga acccggcgaa ctgaccaatg tcgaacaagg cgaaagcatt tgcgttccgg
    12121 ttgacgacat cagcgactgg atgttcgtgt gcaacggcat tccctacggc ggctttaccg
    12181 tacaggcaat gcgcgggcag atgacggaag aggagcgcac cgaacacgat gccgcatggg
    12241 gaatcgattt cggcgatccc gggcagatat tgctggtgta tgaagaaaaa gaacatcccg
    12301 aaaatctgga agagcatccg atgtgccgga actgtattga cgattttcgg caacagttgt
    12361 cccaaaattc ggattttctg catgagcagg acgaagacgg ctatacgccg cttcatcatg
    12421 aagccatcgc aggaaatgca cttatggttc aagccatgct tgaatatggc gcaagtcctg
    12481 cctcaacgac atcggaaggc tataccgccc tcgattttgc ccgcctggcg ggctggcaaa
    12541 atgttgccga cctgctcgaa ccgagacatt aggcagacag ttttccgaaa aacggacgca
    12601 accacttttt acagaaagac aataaaaatg cccaaaatta ccgtacttcc acacgcgacg
    12661 ttatgccccg agggtgcagt catcgataac gcgccggaag gcaaaaccgt cctcgacgta
    12721 ctgctcgacc acgacatcga agtcgatcac gcctgcgaaa aatcctgcgc ctgcaccacc
    12781 tgccacgtca tcatccgcaa aggtttcgac agtttggaag aaccgaccga attggaagaa
    12841 gacctgctcg accaagcatg gggtttggaa gccgattcgc gcctgagttg tcaggcggtt
    12901 gtcgccggcg aggatttgat tgtggaaatc cccaaataca ccatcaacca cgcgcgcgaa
    12961 gaacactgaa aacaggccgt ctgaagccgg cacgcttcag acggcattgt tgcgcggata
    13021 aggcgcaatc gcccgaaaac aggcgttcgt acaggcggaa ctttcgattc tatagcgaat
    13081 taacaaaaat caggacaagg cggcgagccg cagacagtac aaatagtacg gcaaggcgag
    13141 gcaacgctgt accggtttaa atttaattca ctatatattg atttttatcg gttttctgac
    13201 ggaacggatt ggtgcggcat ccgaggcgga ttactcgggc gcgatgcacg ggtatttatc
    13261 ggttttgcag ccggaaaaac cgccggcggg ttactttcag ggcggcggtc gggcggtatg
    13321 ccggatagcg ttccactttc gcttctatat tgattttgat tggttttctg acggaatgac
    13381 ccgatgcggc atccgggacg gcttgtgttt tttcctgccc gcctgccgga ttttcccatc
    13441 cttgcgtgaa accgaaagag acggcggcgc ggcggacaag ctcgagatag cgtccttcaa
    13501 gctccgggca ggcggcggac acgctttctg ccgtaaccgt gaagccgccg cccgacccgg
    13561 aaaggcgttc cgcaatctgc gtgtagatac gccaacgttg ggcgcgcaac attgccgcat
    13621 cgcaaccggc agccgcttta tccaaggcaa tcaggccgcg ccggttttgg tggtgaaggc
    13681 aaaccgttac tctggagagg atatgttgcc cgtccaatat ttgatacagt ttgcgtatca
    13741 gcagaatcag gcggtcaaac tcctccatct ccgacaggga atcaggatgg tcggaagcgg
    13801 tataaaccag tttggacaat tttgcggcaa acatattgtt catcaatctt ccttgtcggt
    13861 tgaaaccccg ctcttcgggg cggtagaatc agatttgttt gggagggaca actcctccca
    13921 gatcaggacg acacataggc tggtgcttga tgtgttgtcc ggcgagttga aacattcagc
    13981 catcctcaag gtgcggcagt tttgccgaaa catattctac acggcttcaa tgccggacga
    14041 taaaaggaaa ttcatatgaa atggaccgac acccggcgta tcgccgaaga actctatgac
    14101 ctgcacggcg aaaccatcga tcccagaacc gtgcgcttta cccaactgcg cgacctgatt
    14161 atggcattgc ccgaatttga tgacgatccc gcccgttgcg gcgaacgtat tctcgaagcc
    14221 gtgcagcagg catggataga cgaggcagaa taagtttcgg gaatgccgtc tgaaatacgg
    14281 cggtacgcgg ttcgtgcttc tgtttgcagt gggaacggtt ttaccagtct ccttttttca
    14341 gcctatccag ttggcggcgg tcgcgcttgg tcggtctgcc gtcgggatgg gcggaagtga
    14401 tgcggctgaa ttggtcgagc tgtttgcgct cttccctcaa tgctgccgtt tttgcgtctt
    14461 cttcatacag aagccgcgcc tcggatgccg ggcggcgttg gtggttcaaa cctttaacct
    14521 tgattttata gggaagggaa ttgagcgtca ggtcgataat atcgccgatg tctatggttt
    14581 tactgttttt gaccttcgag ccgtttactt gaaccctacc cagttcgatg tgcttttgcg
    14641 caagggaacg ggtcttgaaa aaacgtgccg cccaaagcca tttgtccagc cgcatggcgg
    14701 aagaatcgtg cttgtctttc atacgatttt gtttgaaata attgaatttg tttcgagttt
    14761 agcataagat acgccgccta taactagtat atatgcacta atccactgtt ttccatgctg
    14821 tccgaacaca aaaagagggt gtggaaaagc cgttttggac aataaattaa ctgcggaata
    14881 tgcacaaata gcgtatgata gcggcagaat ctgttgatga gagcttcatt ctatgaaacc
    14941 tgtttttttg gattttgaac aacccatagc cgaactgacc aacaaaatcg atgagctgcg
    15001 tttcgtccaa gacgagtctg ccgtcgatat ttcggacgaa atacaccgtt tgcagaaaaa
    15061 aagcaacgac ctgaccaaat cgattttcag caaactcaca cccgcccaaa tttcacaggt
    15121 ttcccggcat ccgcagcgtc cctatacttt ggattacatt gaggcactgt ttaccgattt
    15181 tgaagaactg cacggcgacc gccactttgc cgacgattat gcgattgtcg gcggattggc
    15241 gcgtttcaac ggacaaagcg tgatggtcgt cgggcatcag aaagggcgcg acaccaaaga
    15301 aaaaatccgc cgcaacttcg gtatgccccg tcctgaaggc taccgcaaag ccctgcgcct
    15361 gatgaagacg gcagaaaaat tcggcttgcc cgtcatgacc tttatcgata cgccgggcgc
    15421 gtatcccggc atcggcgcgg aagaacgcgg acagtcggaa gccatcggca aaaacctgta
    15481 cgaactgacg cgcctgcacg tccccgtttt gtgtaccgtc atcggcgaag ggggctcggg
    15541 cggtgcgttg gcggtcgccg taggcgatta cgtcaatatg ctgcaatatt cgacctattc
    15601 tgttatctcc cccgaaggct gcgcttcgat tttgtggaaa accgccgaaa aggcggcgga
    15661 tgcggctcag gctttgggca ttactgctga ccgcctgcaa aagctggact tggtcgatac
    15721 cgtcatcaaa gaaccattgg gcggcgcgca tcgggatttc gggcaaacca tgaaaaacgt
    15781 aaaagccgtt ttggaaaaac aactgcacga agcgcaaagc atcccgcttg ccgatttgct
    15841 ttcgcgccgt ttcgaccgca ttatggctta cggcaaattt tcggaacaat aattcaggta
    15901 gaacaagcag caagcagttt gtctgaaact gcttgctttt tctttatcgg gacggaaccg
    15961 tgctgacttt agatgcgttt gagcaatgct tgaaggattg ttttcctcaa ggtctgaatg
    16021 gaaaaaaaac agcggtggca ttaagcggcg gcttggattc cgtcgttttg ctgcatctgc
    16081 ttgtccgcgc cgggaaaaaa ggcggtttta ttccggatgc attgcatatc catcacggct
    16141 tgagtccccg tgccgacgat tgggcggatt tctgccaaaa ctattgcgat atgcttgggg
    16201 tggggctgga aacggttaag atctgcgtgg aaaaaaacgg tttgggcatc gaggcggcgg
    16261 cgcggcaaaa gcgttatgcc gcgtttgccg aaaaaggctt tgacgttttg gcgttggcgc
    16321 accacaggga cgatcaaatc gaaaccttta tgctggcggt cgcgcgcggc ggcggtttgc
    16381 gcgctttggc ggctatgccc gccgtccgcc cttttgggga aaaaggcatt atctggcggc
    16441 ccttgctgcc tttttcgcgc caagatatat gggattatgc ccaaaaacac ggtttgccga
    16501 atatcgagga cgaaagcaat agcgatacgg cttatttgcg aaaccgcttc cggcaccgta
    16561 ttttgcccga actttcggcg cagattcccc atttcgggcg gcatctgttg aacaatgtcc
    16621 gtgccttgca ggaggatttg gctttgttgg acgaggtcgt cgttcaagac tgccgttggg
    16681 tttgcggagc cggttatttc gatacggcgc ggtggctgac gttttccccg cgccggaaaa
    16741 cccatatttt gcggcatttt ttgaaggaaa acggcattcc cgtgccgaat cagaatgccc
    16801 ttgccgacat tgcccgggtt ttgacggagg caaaaaccgg acgctggaac gtgcaaggct
    16861 ttgaattgca tcattatgca ggcaggctgt ttgtgttccg accggaaaaa acggataaac
    16921 tgcggttttt gaaagacagg cagataagcg gaaatttaag ggaaatattg gcggggcagg
    16981 gatttgtttt aaaacgtcat ccgttcggac ttcccgggca tcttttggag caggacggaa
    17041 tcttgcggac ggttgcggca tcggacaggc tggctgtcgg cggtatccat aaggatgtga
    17101 agaaaatcct tcaggaaaag cgggttttgc ccgcgctgcg cccgttttgg ccggtcgtcg
    17161 ccgacaagga caaccgtccg ctggcgttgg caaattacgg tacggatttt caaatctcgg
    17221 tttcagacgg cattttgccc gtccatccgg attttcccgt tttattttga tggaatcgga
    17281 atcgccgtta cctattatat attcttgaaa aggcggtaaa gtgaagcata tcagttcggc
    17341 aaacaatgaa cacatcagac acctgcaccg cctgttgtct caagggaagt tcagacggca
    17401 atacgcccaa accgttttgg agggcgtgca cctgcttcag gttttcctgc aatccggcgg
    17461 gatgccggtc ggggtatata ttcccgaagc gaaaatgccg tctgaagaag tccgtaaatt
    17521 gacggcggtt ttgccggaag acggggtttt ttccgtttca gacggcatat tgaagaaaat
    17581 cagcagcctg acttgcgcgg atgatgtgct tgcgctgatt gatattccgg atgcgggtgc
    17641 tttgccggcc ggcggcgact gtgtggtttt ggacggcgtg caagatccgg gcaatgtcgg
    17701 cacggtgttg cggagcgcgg cggcggcagg aatcggcgcg gtcgttttgg gcaaaggttg
    17761 tgcggacgcg tggtcgccca aagtgctgcg agccggaatg ggcgcgcatt tcttgtcgga
    17821 gatttatccg caggcggatt tggaaatatg gttggcgcgt tatgaagacc gtgtgtttgc
    17881 caccgcgttg cgtgaggaaa aacaggcggt tctgtataac gaagatttgc gcgaaccgac
    17941 agcctgggtg tttggcaacg aaggcgcggg tgtcggcaaa gcggttttag acagggcgga
    18001 caagtgcgtc aggataccga tgcacgatgc aaccgagtct ttaaatgtcg cgatggcggc
    18061 gacaatctgc ctgtttgaac agatgcggca gcggtcggcg tattgaggaa aagaaatgcc
    18121 gtctgaaaaa atctattacg gcgtattgat tttcttatgt atcgcttcta tgctgctgtc
    18181 gccgtttttt tatgcgggtg ctttgaagcc caagaaggcg gcattgcgga aggacgggca
    18241 gtggaaactc atcttattgt ccaataccgt ggcggcggcg gttttggctt gggtgtggtg
    18301 gaaatggttt tgacagatat tgctcaaaat cgtgctaatg gaatccgaac aaataaagaa
    18361 ttttgtaaaa aatttgttaa atcaacgaat taaagttttg tggaaaacaa aacagctcta
    18421 agcaaatagg gcgtttgtcg gtaaatacgg aagagttgcg gcattatcgg gcatctttaa
    18481 caagtagtgc cgtcttggca ggcaatcggt ttttatgggc agcttgcaaa atcgcggata
    18541 taaaattgcg aatcggttaa agtgtgggga cgctatgaaa aattgcgaat tttttatgac
    18601 ccgacaaggg caatctatga tagcggtgca gattatttaa ccagagaaaa acatagattc
    18661 gtcgtaattg caaatagtgc ttgggggcta ttgcttaatt tatcttgtta ttatgacaag
    18721 gttttggaaa agcggaaaat accgttcggc aaacaggaaa ttgatgacga tatggacaaa
    18781 gtgtccgccc ataagcggaa gtttaaagat atttctgaaa tcaaagtagg ggatggttgg
    18841 gaatacccgt tcaattatga gcagggaatg aaagaattag atgaagtgct attgaaatac
    18901 attccctttt ttgaagaaga acgataaagg aggttgatat gcgcgtatct aaaataattg
    18961 gaagtatgtt gcttgttaca gcggttcaga ccgtattttc ggcaaatgtt tacgcgtgcc
    19021 gccataatgg taaaaccagt tacagccaaa ctccgggaaa acattgtacc aacgcgggtt
    19081 tggggcggga tcgggtgtac agttcggtta gaccggcagt aaaagacagg gcggaagacg
    19141 caggggtcgg cgattattcg gacacggtga gggacgaaca cgtccaaaat ccgagagaga
    19201 atacgcataa ggatgcttcg aataccggtg caaagacgca ttgattgaaa ccgaatcagc
    19261 catgacagcg ttgaataata gaatttgaac gattagggaa ctctgatgaa acacatccac
    19321 attatcggta tcggcggcac gtttatgggt gggattgccg ccattgccaa agaagcaggg
    19381 tttgaagtca gcggttgcga tgcgaagatg tatccgccga tgagcaccca gctcgaagcc
    19441 ttgggcatag gcgtacacga aggcttcgac gcggcgcagt tggaggaatt taaagccgac
    19501 gtttacgtca tcggcaatgt cgccaagcgc gggatggatg tggttgaggc gattttgaac
    19561 cgcgggctgc cttatatttc cggtccgcaa tggctggctg aaaacgtgct gcaccatcat
    19621 tgggtactcg gtgtggcggg gacgcacggc aaaacgacca ccgcgtccat gctcgcatgg
    19681 gtcttggaat atgccggact cgcaccgggc ttccttatcg gcggcgtacc ggaaaacttc
    19741 agcgtttccg cccgcctgcc gcaaacgccg cgccaagacc cgaacagcca atcgccgttt
    19801 ttcgtcattg aagccgacga atacgacacc gcgtttttcg acaaacgctc caaattcgtg
    19861 cattaccgtc cgcgtaccgc cgtgttgaac aatctggaat tcgaccacgc cgacatcttc
    19921 gccgatttgg gcgcgataca gacccagttc caccacctcg tgcgtaccgt gccgtctgaa
    19981 ggcctcatcg tctgcaacgg acggcagcaa agcctgcaag acactttgga caaaggctgc
    20041 tggacgccgg tggaaaaatt cggcacggaa cacggctggc aggccggcga agccaatgcc
    20101 gatggctcgt tcgacgtgtt gcttgacggc aaaaaagccg gacacgtcgc ttggagtttg
    20161 atgggcggac acaaccgcat gaacgcgctc gccgtcatcg ccgccgcgcg tcatgccgga
    20221 gtcgacattc agacggcctg cgaagccttg agcacgttta aaaacgtcaa acgccgcatg
    20281 gaaatcaaag gcacggtaaa cggcatcact gtttacgacg acttcgccca ccatccgacc
    20341 gccatcgaaa ccaccataga aggtctgcgt cagcgcgtcg gaaacgcccg catcctcgcc
    20401 gtcctcgagc cgcgttccaa cacgatgaag ctgggcacga tgaagtccgc cctgcccgaa
    20461 agcctcaaag aagccgacca agtgttctgc tacgccggcg gtgtggactg gaacgtcgcc
    20521 gaagccctcg cacctttggg cggcaggctg cacgtcggca aggatttcga tgccttcgtt
    20581 gccgaaattg tgaaaaacgc ccgaaccggc gaccatattt tggtgatgag caacggcggt
    20641 ttcggcggaa tacacaccaa actgctggac gctttgagat agcccgggcg atgccgtctg
    20701 aaagcccttc agacggcatc gtccggctgc gcggcacaaa ggcggaaaaa ccgtttgccc
    20761 cgtattttca aacgcgttac acttgccgcc gctgttttca gccatttgat tacccgcaac
    20821 cgccgtcatt gcgccggcgg tttgcctgtc agcgtcattg cgccgctgta aatacgaaag
    20881 aacacattat gaccgtatcc cccgtcgcct tgcgccgtaa gaccgagtgc aagcctcatc
    20941 ccaccgcgcg ctattggaaa aaatgcgatg tcgaagccct gttcggactt cccttcctcg
    21001 acctcattta ccaagccgcc gaaatccacc gccaaaattt caacccgcgc gaaatccagc
    21061 tttccacgct gttgtccatc aaaaccggcg gctgtcccga agattgcgcc tactgtccgc
    21121 aatcggcgca ccacaacacc aatctgggca aagaacagat gatggatgtg gatgaaatcg
    21181 tcgaaaaagc caaaatcgcc aaatcgcgcg gcgcaagccg gttttgcatg ggcgcggcgt
    21241 ggcgcggccc caaacccaaa gacgtggaga cggtttccgc aatcatcaaa gccgtcaagg
    21301 gcttgggtat ggaaacctgc ggcacgttcg gtatgctcga agaaggtatg gcggaagact
    21361 tgaaagaggc gggcttggat tattacaacc acaacctcga caccgacccc gaccgctaca
    21421 acgacatcat ccatacacgc cggcatgaag accgtatgga caccttgggc aaagtccgca
    21481 acgccggttt gaaagtctgc tgcggcggca tcgtcgggat gaacgaaacc cgcgccgaac
    21541 gcgcagggct gattgccagc ctcgccaacc tcgacccgca gcccgaaagc gtgccgatta
    21601 accggttggt caaagtggaa ggtacgccgc ttgccgatgc cgaggatttg gactggacgg
    21661 aatttgtccg caccatcgcc gtggcgcgga ttacgatgcc gcaaagttat gtccgactgt
    21721 cggcagggcg cagcaatatg ccggaagcga tgcaggcgat gtgctttatg gcgggcgcga
    21781 actcgatttt ttacggcgac aagctgctga ccacgggcaa tccggatgaa gacggcgaca
    21841 gaatcctgat ggaaaagctc aacctgtatc ccttacagtt tgagctggaa ggcgaggtcg
    21901 ccgaagtgga aaaagcctct gggattaaag tggattattg acgattgcaa aaatgccgtc
    21961 tgaaacccga aaaaaggctt tcagacgaca tttgttcgga cggtatttcc aatatctttt
    22021 taccggcgcg tgatgttgcc gtcgggcgag acatccagcc cgttctcgtt ggggaagtcg
    22081 ctgcggttca gataaataat ccgcccgata acggtttttc agggtcgatt ttggggattt
    22141 tatcgtcggc ttgggtaata gggatgatag ttccggaaat aaatttaccc tgtttgttgg
    22201 tgataatttt gaaaatcggc gcaatgccgc tgatgccgga ggtgttgatt gcgccgtagg
    22261 tggcaaagtt gccgccgctg taagagatga agcggtcgcg gtaaagttcg acggcgcgtg
    22321 taacgtgcgg cccctgcccg aatacgacat ccgcgccgga atcgacggca agccgtgcaa
    22381 actcgacgac gttgcccctg ttttccccat agaagatttc ggtatcgaac ggcaggtgtt
    22441 ccgcctgttt cccttccgcg ccgccgtgga acattacaat gacgatgtcg gctttctgtt
    22501 tggttttggt aatccgtttt ctaactttgg cataatcgtt cagtttgacg gcggcaaggt
    22561 tgggggcgaa ggagacgaag ccggatctta cgccgttttt cttcaggatg gcggtttcaa
    22621 acctgttttc gatgcccgaa tatttgatgt tcaattcgtc aaggttcgcc ctttatgccg
    22681 tttccgcacc gcaaaccgcc ggggtcaagc cctcggcgca tcctgccgga acggaatccc
    22741 cgtgctgccg attgacggtt caaaccccgc gcccgtttca aataccggcg atgtggacgg
    22801 acaggatgcg cctgacgaaa agacagccga taccgtttcc attatcggcg tgggcgacat
    22861 tatgctcggc agcaattatc cggtcgatta cctgcccgat accaatattc tgaaaaacgt
    22921 cgaatctgcc ttgcaagacg cggacattac cgtcggcaac ctcgaaggca cgctgtttga
    22981 cgaaggcggt acgccgaaaa aatgtgcaac cccccaaaat atgctatgca ttccgaacgc
    23041 cctccgcata cgggcaatac cttgccgacg cgggattcga ctacctcagc ttcgccaaca
    23101 accacagcaa cgacttcggc gcgcaaggca tcacggcaac ggcggcgagc ggcagctctt
    23161 ttacatactc gatcgcgcta aagccgctgc cgataacgag gccaaaattg cggaaaatac
    23221 cgccatcgcc cagataaatt tgtccatcat cagaccttta ctgttcagac gagacagcat
    23281 ttgccgcacg ttttggggct tatctttcga tttgcgctat gtcgcgcacc gcgcctttgt
    23341 cggcggaagt cgccatcgcg ccgtaagctc ttaatgcggc ggagacgtag cggtcgcggt
    23401 ttttaggctt ccatgctttg ctgccgcgcg cttccatttc ggcacggcgt gcggcaagct
    23461 cttcatcgga aatggcaagg tggatgctgc ggttggggat gtcgatttcg acggtatcgc
    23521 cttcgtgtac caaaccgatc gcgccacctt ccgccgcttc gggcgaggcg tgtccgatgg
    23581 acaaacctga tgtgccgccg gagaagcgtc cgtcggttaa gagagcgcag gctttgccga
    23641 ggcctttaga tttcaggtag gaagtcggat acagcatttc ctgcatgccc gggccgcctt
    23701 tcgggccttc gtagcggatg atgacgatgt cgccggcgac gatttggttg cccaaaatgc
    23761 cttctactgc gtcttcttgg ctttcaaaca cgcgggcgcg gccggtgaat ttgaggatgc
    23821 tctcgtccac gcctgcggtt tttaccacgc agccgcgctc ggcgatgttg ccgaacaaga
    23881 ccgccaaacc gccgtcttgc gagtaggcgt gtgccacgtc gcggatacag cctttttcgc
    23941 ggtcgaggtc gagggttttc cacatacggt tttgcgagaa cgcttgggtg gtgcgtacgc
    24001 cgccgggcgc ggctttgaag cgttcgatgg cgcgggtgtt ttcagggttg gttacgtccc
    24061 atttttcaat cgcgtctttc agcgtcggtg cgtggatagt gtgcacgtcg gtgtgcagct
    24121 tgcccgcttt gtccaattct ttcaggatgg cgaaaatgcc gccggcgcgg tgcacgtctt
    24181 ccatatagta gtcgtggttg ttgggtgcgg ttttgcagat gcagggcaca acgcggctta
    24241 agcggtcaat gtcggccatt ttgaaatcga caccggcttc gttggcaacg gccaacaggt
    24301 gcaaaatggt attggtgctg ccgcccatcg caatatccat cgtcatagcg ttttcaaacg
    24361 cttttttggt ggcaatgctg tgcggcagca cggtttcgtc gttttgttcg taataacgtt
    24421 tggtgatttc gacaatcata cgtcctgctt cgaggaacaa ttctttacgg cctgcgtggg
    24481 tcgccaaata cgaaccgttg ccgggtaggg acagaccgag tgcttcggtc aggcagttca
    24541 tcgaatttgc cgtaaacata cccgaacacg aaccgcaggt cgggcaggcg ttttgttcga
    24601 cttcctcgac ttgccggttg ctgacattgt cgtccgccga ttcgatcatc gcgtcaatca
    24661 agtccaaacg gcgttcgggt tggatgtttg ccacgccgat aactttgccc gcttccatcg
    24721 ggccaccgga aacgaagatg gtcgggatat tcaggcgcat ggcggcaatc agcattcccg
    24781 gggtgatttt gtcgcagttg gaaatgcaca ccagcgcgtc ggcgcagtgg gcgttgacca
    24841 tgtattcgat ggagtcggca atcaaatcgc ggctgggcag ggaatacagc atgccgctgt
    24901 gtcccatagc gatgccgtcg tcgatggcga tggtgttgaa ttctttggcg atggcgccgg
    24961 ctttttcgat ttcgcgggca accagctggc ccatattgtg caggtggaca tgacctggca
    25021 cgaattgggt gaacgagttg gcaacggcga taatcggttt gccgaagtcg gtttccatca
    25081 cgccggtggc gcgccacaat gcgcgcgcgc ccgccatatt gcgaccgtgg gtggaggttt
    25141 tggagcggta gtccggcata gtgtgtttcc ttgtctgcta tgccgtctga acggcaaaag
    25201 gctttcagac ggcatttttg ttgtgttttg aaatgaaaaa aaaaggtatt ttataccaag
    25261 tatcggaatt ttgcgggatt gaaacggcgt gcggcaaaaa agaaaatccc cgcaggaatg
    25321 cggggacggg ttcaggcgcg ggcaatcgcg acggctttgg atgcgtccca aaaatcaacg
    25381 ggggcattta atacgggttt gacgatgccc gtccgtatgg cgaaatcgcc gaaaccttcg
    25441 ccgatattgc gttctgccgc ccatttgccg atcaggtcgt ccaattcggc aaggatttcg
    25501 ggcagggtga tgttttcttt gtaaaggcgg gggatgcgta cgccttcacg gtcgccgccg
    25561 atatggaggt tgtagcgtcc gacggctttg ccgaccagcc cgatttccgc caacattgcc
    25621 cgtccgcagc cgttggggca gccggtaatg cgggtaacga tatagtcgtc cgacgtgccg
    25681 tgtttcgcca taatcttatc cagatcgtcg atgaagtctg gcagcacgcg ttcggcttcc
    25741 gccattgcca aggggcaggt cggaaaggaa acgcaggaca tcgcattttc gcgcagcttg
    25801 ctgacatcgt tgcggattaa tccgtatgtt cgggcaagtt cttcgatttt tgctttgtct
    25861 gcttcggcaa catttgccac gatgaggttt tggtttgcgg taatgcggaa atcgcctttg
    25921 tggattttgg cgatttccaa cacgccggtc agaagctgtt tcccgccttc gtcaaccaaa
    25981 cgcccgcttt cgatgaaaag ggtcaaatgc cagttgccgt ctatgccttt cacccagccg
    26041 atgcggtcgc cgcgcccggt aaatttgaac gggcgtatgg gttcgaacgg catacccata
    26101 cggcgttcga cttccgcgcg gaagttgtcc aagcccatat tttgaatggt gtagcgggtg
    26161 cgggcgtttt tgcggtcgct gcggttgccg aagtcgcgct gtgtggttac caccgcttcg
    26221 gcggccttca gcgcgtgttc cggaggtacg aaacccagtt ccagtgaaat gttcggatag
    26281 gttttggtgt tgccgtgttc cattgaaagc ccaccgcctg ccaaaacatt aaaaccggca
    26341 agctgtccgc cgccgtctga aatggcgaca aaatccaagt cgttgccgta gcagtccaca
    26401 tcatttaagg gcgggatgac gactgcggtt ttgaatttgc gcggcagata ggttttgccc
    26461 aaaatcggct cgtcttcttg aaggaagtcg tcggaacttt gaactttttt gccgtccacc
    26521 caaacatcca gataaccgcg cgtgcgcggc agcaagtgtt cggaaatctt tttcgcgtat
    26581 tcgtaagcct gttggtggag tgccgactcg atgggattgg aggtgcaaag cacgttgcgg
    26641 ttcatatccg ccgccgtggc gatggaatcc aaacccagtt tgtgcaagag gcggtgcatc
    26701 gtctgcaact tggttttcgg cacgccgtga aattggaagg tttgccggtt ggtcagcctg
    26761 atggatcggt aataactgtt ttcgcgggcg aatttgtcca attctatcca ttgggacggc
    26821 ttgatgattc cgcccggcag ccggcagcgc aaaagcataa atttcaaggg ctcgagtttt
    26881 gcctcggcgc gttcggcgcg gatgtcgcgg tcgtcctgct catacatacc gtggaagcgg
    26941 atgagttgga agttgtcgcc tttgaagccg cccgtgaggg ggtctttcaa atcgtccaaa
    27001 atcgtgccgc gtaaaaaatt gctttcggtt ttcagacgtt cgttgtcgga tagcggtttt
    27061 tcttgccacg ccaaaccttt tgtcttggtc tgtacggtca ttttgtgttc ctcccgatta
    27121 tatttaatca ataaacatca cgctgatagc gtttttcttc gcgcagcata tccaaatatt
    27181 cttctgcgcc ctcttcgtcc aaatgtcctg ccccgataat cacatccagc aaggcggctt
    27241 ccacgtcttt tgccattttt gccgcatcgc cgcacacata gatatgcgcg ccttcctgca
    27301 gccattgcca aagtccttcc gcctgttcgc ggattttgtc ctgcacatag attttttctt
    27361 cctgatcgcg ggaccaggcg aaatcgtatc tgtgcaggaa gccgtctttg gcaaactgct
    27421 gccattcggt ctgatagagg aagtcggcgg caaaatgcgg attgccgaaa atcagccagt
    27481 ttttgccttc cgcattttct gcggcacgtt gttggacgaa agcgcggaac ggtgcgacgc
    27541 cggtgcccga gccgatcatc acaatcggct tgcggctgtc ttcgggcagc ctgaagccgt
    27601 cgttgcgttc cacaaacacg cgcaccgtgc cgtcctcttc cagccggtcg gcaaggaaac
    27661 ccgatgcgcc gcccgttctg gcgcggcctt cgtgttcaaa acgaaccacg ccgacagtta
    27721 aatgcacttc atcgcccact tccgcctgtg ctgaagaaat cgaatacaaa cggggtgcaa
    27781 gcggacgcag taaacggatg aattgttctg ccgtcaggct tgccgggaag cggtgcagca
    27841 catcgacaat aggcgtgttt tgcacgaaat cctgcaaaac ggcgttatcg gcaatgattt
    27901 tatcgagttc ttcataatgg gcgaacgcgg catagccttt gacgaaagcc ggagtgtttt
    27961 gcgtgagttc gaaatgagat gaaagtgcgc gcgcaaccgg catcatcttt ccgcctgcct
    28021 gtatttccgt tgccggatcg atgccaagca ggtctaggat ttccccgacc agtgccggat
    28081 tgttgtcaaa ccaaacgccg agcgcgtcgc ctgcctgata gcttaaatcc gaaccgctca
    28141 aatcaagttc gatgtggcgc acgtctttat cggattggcg ggcggtgatt ttctgattgg
    28201 ccagcagtgc ggcgggaaag gggtctgcct tgcagtacct gccctccgat gccgtctgaa
    28261 ggccggcggg gggcgttgcc tgcggcgcgg gcgttgcccg gttttttgcg gcttcttctt
    28321 ttaagagtgc ggcgatattg tctgtccagg cgtttgcaga ggcggcaaag tccaaatccg
    28381 catcaacgcg ttcgagcagg cgtttcgccc ccaattcttc aaaacgtttg tcgaaatctt
    28441 tgcccgcccg gcagaaattc ggataggaac tgtcgcccaa acccagtacg gcaaattgga
    28501 gtttgtccaa tttcggggct tttttgccgt tcagcagttt gtgcagcacg acggcttctt
    28561 ccggcggttc gccttcgcct tgggtggacg taaccagcag caggcggcgt tcgccggcga
    28621 tgtttttcgc cttatagtct ttcagttcgg cgcgactgac ttggatgccg gcggcttcca
    28681 ggctgtccgc cgctttgtcg gcaacggatt tcgcattgcc ggtttgcgag gcggaaagga
    28741 cggttacgga aaaaggttct gccgtcggca atgctgtctg aagcgcgggc agccctgcgg
    28801 atgccccgtt tcctgctttt gcccaagcat agccggacag ccacgcccat tgtgccgcgt
    28861 ccagccccga caggagctgc gtgatttcgg gcggcagagg cggtaatggc ggatttgtgt
    28921 tctgcatatc gtgttcactc ataaaatcat acctgccgca acagtgccgt atgtcgattc
    28981 gtctatcagg ataaacgaac cggcggcggt gttttccgca taaggcgttg ccgtaacggg
    29041 tttttgaagg ttgatgcgga ctttggcgat gtcgttcatc ttcaaggatt ccgcgccggc
    29101 ctcttgttcc agcgtgcgga catccaaaac gctttcaatt tccccgactt ttgccggcac
    29161 ggtttgcgtg ccgtgcttga gcaggtattt gcgcgcggtg ttgagcggac gttcgtcaaa
    29221 ccagcaaagc gtggcttcca gatgtttttg cggggcgagc ggggaatttt tatcgacaaa
    29281 aaggtcgccg cgcgaaacat cgatgtcgcg gtccagccgg agggttgccg cctcgccggc
    29341 aaaagcctgc gccacttccc ctttcggcgt gatgatttcg gacacttcgg cggtcagccc
    29401 gttcggttcg atgcggacgg tttgcccgac ggcgaccgaa ccgcgttcga tgcgcccctg
    29461 atagcctcgg aaatcatcgg ccttgtcggc atcttggcgg acgaccagtt gcacggggaa
    29521 ataaaaatcg tcggcggtgc ggctgacttc gtccgccccc ggcagggttt ccaaaatgga
    29581 caataagggt tcgcctttat accaaggcat attgccgccg gggtaaacaa tgttgtcgcc
    29641 caagagtgcg gacaccggta cgaaatgcgc gtctttcaaa ccgagctgtt cggcaagtcg
    29701 gcggtatgcc tccacaatgg cgttgaattt gtcttcgctg taatccagca ggtccatttt
    29761 gttgaccgcc accacaatat gcgggcagtt gagttggcgg aggatggcgg aatggcgttt
    29821 ggtctgcggc agaagctgta agggctgcgc gccgaaatcc agttgggatg cgtcaaccag
    29881 tacgactgcc gccgaagcgg tgcttgcgcc cgtaaccata ttgcgcgtgt attgttcgtg
    29941 ccccggcgtg tcggcgatga tgaatttccg tttcgccgtg gaaaaatagc ggtatgccac
    30001 atcgatcgta atgccctgtt cgcgttcggc ttccagtccg tcggtcagga tggagaagtc
    30061 tatggtttct ttcaaacctt tgcttttgcc ggattccaag gttttgattt ggtcggacag
    30121 cagggctttg ctgtcgtaga gcagccgtcc gatcagggtg cttttgccgt catcgacgct
    30181 gccggcggta atgaagcgga gcggggcctg gtgttgtgcc gtcatatttt cttcctcata
    30241 tctgcttaaa gggtttttga aatttagaaa tagccttctt ttttgcgttt ttccattgcc
    30301 gcctcgcttg cctgatcgtc cagccgggtc gcgctgcgtt cggaaatgtc ggcaaccgct
    30361 gtttctctga taatctccgt cggcgtggac gcggtgcttt ctaccgggca ggtgcagctg
    30421 atgtcgccga cggtgcggaa gcggacatca aggatttcgg aggtttcaga cggcattttc
    30481 ggggtgagcg gcgttacggg gaccagcagc cccctgcgtc tgaccacttc gcgcctgtgg
    30541 ctgtaataaa tcggcggcag ctcgaggttt tcgcgggcga tgtattgcca gatgtcgagt
    30601 tccgtccagt tggaaatcgg gaagacgcgc atattttcgc ctttgtgcag cctggtgttg
    30661 tacagcgacc acagctcggg gcgttgcgcc ttcggatccc attgtccgaa ctcgtcgcgg
    30721 aacgagaaaa tccgttcttt ggcgcgggct ttttcttcgt cgcgccgcgc gccgcccatc
    30781 agcgcgtcaa agccgtttgc ctcgatggtt tccaacagcg taaccgcctg cgccgcattg
    30841 cgcgaatcag tttctttgcg taaaactact gtgcctttgg caatggagtc ttccacgcgc
    30901 cccactatca ggcgggcatt gagttttgcc gcctgcgcgt cgcggaaggc aatcacttcg
    30961 gggtagttgt gccccgtgtc gatatgcacc agcgggaagg gcagttttac cggcctgctg
    31021 cccagccgga aggctttgca ggcgagggcg agcaggacca cggaatcttt gccgccggaa
    31081 aagagcaggg cggggttttc gcattctgcc gccacttcgc ggataatgtg gatggattcg
    31141 gattccaacc agtcgagttg ggcgttgttc ggttcggttt tcgtcatacc atattcctta
    31201 tttcttctgt ctgatattta tgaattattt gtgcagcccg cattctttgc tgtttctgcc
    31261 ttcccaccac caccgcccgg cgcggatgtc ttcgcccgcc ttgacggggc gggtgcaggg
    31321 gtcgcagcct atgctgggaa atccttgccg gtacaaatcg ttgtaaggca cattgttggt
    31381 gaggatgtat gcccacacgt cgtgttccga ccagtcgaaa atcgggttgt atttgccgat
    31441 gtcccgtccg gcatcgtatt cggcaaacgg cagttccgtg cgtgtggcgg actgttcgcg
    31501 gcgttgcccg gtaagccagg cgtccgcgcc tgcaatggcg cggttgagcg gttcgatttt
    31561 tcggatgcgg cagcattcgc ggcgcgcttc aacgctgtcg taaaaggcaa acctgccttt
    31621 gctttccaca taacggtcgg catcttctcg aaccggccgg aaacgcttta tccgcaaatg
    31681 gggatatgcg cgtccgagtc tgtccagcag gtttagggtt tccgtgtgga gcagccccgt
    31741 atccaaggta aaaatgccga tattgaggtt ttcgccggcg ataaggtcgg taatcaccat
    31801 atcttctgcc gcaaggctgc tggcaaaccg tgcatcccgg tggctgccga caatccggtg
    31861 caggcgttgt ttgagggttt ccgttttttc cgcaagggcg gtttcgccgc cggatccgat
    31921 atgcggtatc tgccacaggg cgggtttgaa cagtgtcgtt tccatttttc ccgccttatg
    31981 ccgcccgttg tccggcattc agtccgccca atgcgggata cgtctgcccg acccggtttt
    32041 ctccttcgcc gttttcaccg aaccaggcga gtttttcgtg cagccccacc acttcgccta
    32101 tgacaatcaa tgccggattc ggcgcggttt cggcgagttc ggcaaggttg gcgagcgtgc
    32161 cggttgcggt tttttgagcc ggcagcgtgc cttggctgat gacggctgct ggcgtgtcgg
    32221 gcgagcgtcc gtgctgttgc agccgttcgg caatcagggc ggctttgagc gcacccatat
    32281 aaatcaccaa ggtctggcgg ctgcgggcga gggtctgcca ttcgatgtcg ggcgcatccg
    32341 ccttgcggtg gccggttacg aaaaccgcac tttgggcata atcgcggtgc gtgagcggga
    32401 tgccggcata ggcggtcgcg ccgacggcgg cggtaatgcc ggggacgacc gaaaacggga
    32461 tctgatggcg tgccaaggtt tccaattctt cgccgccgcg tccgaacacg aaagggtctc
    32521 cgcctttcaa acgcaccacg cgcctgcctt cgcgggccag cctgaccata agcgcattgg
    32581 tgtcctcttg cggggtgcgg tcgccccggg cgcgcttgcc gacaaaaatc cgttccgcat
    32641 cgcggcggac gagggacagt atgccgtctg aaaccagcgc gtcgtaaagc accacgtctg
    32701 cctgctggat ttcctgcagc cctttgagcg tcagcagccc cgcatcgccg ggacccgcgc
    32761 cgaccagcga gacggagccg ccttgatcat tttgacgact ttgttccaat tggcctgcca
    32821 attcccgttc ggcaagggtg ttttgccggt ttttgacgag ggcggcgaaa cgtccgttaa
    32881 actgcttttc ccaaaagcgg cggcgttcgg taacggattt cagtttgccc ttgacggcat
    32941 cgcgccacct tcctgaaatt tccgccatat cgcccaaaga cggcggcagc agggcttcca
    33001 gcctttcacg cagcagtcgg gcgaggacgg gcgcgctgcc ggagctggaa acggcaatct
    33061 gaaccgggtt gcggtcgata accgacggga agatgaagct gcaatggtcg cggtcgtcca
    33121 ccacgttgac cggcttttgg cagctttcgg caagatggaa aacgcgccgg ttgagggctt
    33181 ggtcgctgct tgccgcaatg atgaggaaaa ccgtgcggat gtgttcggca cgaaattctt
    33241 cggcaagcca caggattttg ttttccgccg ccaacgcgga gagttcggca ttcaggtgtt
    33301 ttgcggcaac cctgacctct gcgcccgcct tcagcagcag gctgattttg cgtgcggcga
    33361 ccgcgccgcc gcctacgacc aatacggggc ggccggcgag gttggcgaaa atagggaaat
    33421 aattcactgg ctgactcctt tgctgtttgc ccgcaccttg tttccgatac ggtgcgtcgc
    33481 ggcatttttg tcggaatgcg ggtcatttta gacaaaagga ttttcccggt taaataataa
    33541 aaaggcattt gttagaagct gaaagctata tgagggcggc tgcggatacg gcggttttcc
    33601 gttttatagt ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagagaac
    33661 gattctctaa ggtgctgaag cactaagtaa atcggttctg tactatccgt actgtctgcg
    33721 gcttcgtctc cttgtcctga tttttgttaa tccactataa aacggtttgg gacggaaaaa
    33781 cggcctgaag ccgtttcggg cattcagacc gtttgcgcgg agaggggatg ccgtccgaag
    33841 ggcgaaaagg gcttcagacg gcattgatgt cgggtttcag gacaggagca ggatggcggc
    33901 tgcggcaagc gcggcaaccg ataatgcggc ggcaagcgcg gttttaccgg caaagcggat
    33961 tgaggttttg ccttcgatgt atttgaagcc ggttatcatc gggaggacga ggtttttctt
    34021 tttgaacacg cggtatgcgg cgacggcggc gatatgaatg gcggaaaaaa cggcgagcag
    34081 gttgaaaaag ttgaggtgga ttttccgcat aaggctgcct gtatgttcgg aaaccaaatg
    34141 gttgaggtag ccgttggtgc tgaaggtgtt ttcatcggcg gcaaaaagcc ccgtgccgat
    34201 ttgaaatgag acggcggcca aaagcgcgac gaccatcagt gcgcccaagg ggttgtgtcc
    34261 gggctggata tgttcgggaa taccgttttt cagataatcc ctgatacccg accatccgcg
    34321 gacgaaacgg gagaaacggg cggtatcgct gccccaaatg ccccagcaga ggcggaatac
    34381 gagcaggaaa aggataaaca gcccgacgcg cgtgtgccat tgaagcatat cgccgccggc
    34441 ttttgcgcta taccacataa agggcaagga cgcggcaagc agccagtgga aaaggcgggt
    34501 ggggaggtcc cagactttgc ttttgttttt cataatcggt ttccggcggt agaatcggtt
    34561 tgttttcgag ccttaatttt aaccgattgg aggggaaatg tttcccgttt tccatctttc
    34621 aggcgaaagc cgccgccgga tgcttcaggc ggcattgcgt tttccctatg ttttcaaagc
    34681 ccgtgcggaa gattcgcaca aagggacttt cggcacgctc gccgtagtcg gcggatcggc
    34741 agggatgagc ggtgcgcccg tattggcggc atcggcggcg atgtatctcg gctgcggcaa
    34801 ggtgtgggcg ggtttcaatc aggatgcgct tccgtttgcc gtcattgccg gtttccccga
    34861 aattatgttg gacacggcag acggtttgac caagcgtcaa ggcataaacg cctgggttgt
    34921 cggttgcggc ttgggtacgg ataaggcggc ggtcggaacg cttgccggaa ttttgacgga
    34981 acacacggac aagcccgtcc ttttggatgc ggacgcgctg aacatattat caaccgatgc
    35041 cgaaacccga aatctggcac gcgggtgtaa aaacctgatt ttaacgccac accccgccga
    35101 agccgcgcgc ctgcttggaa cgacggttgc acaggttcag gcggatcgga cggcggcagt
    35161 gaggaagata agggcaattt tcggcgcaac cgtggtttta aaggggcaca aaacattggt
    35221 tgcctcaccc gatacggaaa tctatgtcaa cgaaagcggc aacgcgggat tggcaacggc
    35281 gggcagtggc gacgtattgg gcggcatcat cggcagtctg ctcgcacagg gcgtgccggt
    35341 ttttgaagcc gcctgcgcgg gcgcgtggct gcacggcgcg gcggcggatg tcataaaaga
    35401 atcggcaggc attgcggcag ggctgttggc tggcgaaatc gccccggcgg caaggtggct
    35461 gcgcaaccgg ataactgaaa gtatgtaaga agatatagtg gattaacaaa aaccagtaca
//
LOCUS       DAWHXS010000007        32613 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWHXS010000007 DAWHXS010000000
VERSION     DAWHXS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.007"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(316..963)
                     /gene="adk"
                     /locus_tag="WIE22_000264"
     CDS             complement(316..963)
                     /gene="adk"
                     /locus_tag="WIE22_000264"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ4126411.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            1178..2017
                     /gene="htpX"
                     /locus_tag="WIE22_000265"
     CDS             1178..2017
                     /gene="htpX"
                     /locus_tag="WIE22_000265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ4126412.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(2318..2878)
                     /locus_tag="WIE22_000266"
     CDS             complement(2318..2878)
                     /locus_tag="WIE22_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126413.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(2992..3384)
                     /locus_tag="WIE22_000267"
     CDS             complement(2992..3384)
                     /locus_tag="WIE22_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4126414.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(3592..3984)
                     /locus_tag="WIE22_000268"
     CDS             complement(3592..3984)
                     /locus_tag="WIE22_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4126415.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(4101..4511)
                     /locus_tag="WIE22_000269"
     CDS             complement(4101..4511)
                     /locus_tag="WIE22_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4126416.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(4525..4962)
                     /locus_tag="WIE22_000270"
     CDS             complement(4525..4962)
                     /locus_tag="WIE22_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4126417.1"
                     /translation="MKISIFWLYNAFILKRSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(5373..6671)
                     /locus_tag="WIE22_000271"
     CDS             complement(5373..6671)
                     /locus_tag="WIE22_000271"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ4126418.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDVVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(6775..7926)
                     /locus_tag="WIE22_000272"
     CDS             complement(6775..7926)
                     /locus_tag="WIE22_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ4126419.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            8261..9640
                     /gene="norM"
                     /locus_tag="WIE22_000273"
     CDS             8261..9640
                     /gene="norM"
                     /locus_tag="WIE22_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ4126420.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            9819..10859
                     /gene="murB"
                     /locus_tag="WIE22_000274"
     CDS             9819..10859
                     /gene="murB"
                     /locus_tag="WIE22_000274"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ4126421.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            10871..11521
                     /locus_tag="WIE22_000275"
     CDS             10871..11521
                     /locus_tag="WIE22_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ4126422.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            11576..12343
                     /locus_tag="WIE22_000276"
     CDS             11576..12343
                     /locus_tag="WIE22_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ4126423.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGFPAPDFAPDDSMGKIIRTVCDNS"
     gene            12403..12969
                     /locus_tag="WIE22_000277"
     CDS             12403..12969
                     /locus_tag="WIE22_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ4126424.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            12989..13879
                     /locus_tag="WIE22_000278"
     CDS             12989..13879
                     /locus_tag="WIE22_000278"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ4126425.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            13904..14662
                     /locus_tag="WIE22_000279"
     CDS             13904..14662
                     /locus_tag="WIE22_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ4126426.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWESDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(14717..15382)
                     /locus_tag="WIE22_000280"
     CDS             complement(14717..15382)
                     /locus_tag="WIE22_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4126427.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(15582..16478)
                     /locus_tag="WIE22_000281"
     CDS             complement(15582..16478)
                     /locus_tag="WIE22_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ4126428.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVARLTMTVYLPAEQKGTHMS
                     RFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            16547..17704
                     /locus_tag="WIE22_000282"
     CDS             16547..17704
                     /locus_tag="WIE22_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ4126429.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(17767..18768)
                     /gene="hemB"
                     /locus_tag="WIE22_000283"
     CDS             complement(17767..18768)
                     /gene="hemB"
                     /locus_tag="WIE22_000283"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ4126430.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(18951..19235)
                     /gene="yhbY"
                     /locus_tag="WIE22_000284"
     CDS             complement(18951..19235)
                     /gene="yhbY"
                     /locus_tag="WIE22_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ4126431.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            19345..19965
                     /locus_tag="WIE22_000285"
     CDS             19345..19965
                     /locus_tag="WIE22_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ4126432.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            20029..21996
                     /gene="ftsH"
                     /locus_tag="WIE22_000286"
     CDS             20029..21996
                     /gene="ftsH"
                     /locus_tag="WIE22_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ4126433.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            22217..22648
                     /locus_tag="WIE22_000287"
     CDS             22217..22648
                     /locus_tag="WIE22_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ4126434.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            22641..22919
                     /locus_tag="WIE22_000288"
     CDS             22641..22919
                     /locus_tag="WIE22_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ4126435.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            22972..23550
                     /gene="pth"
                     /locus_tag="WIE22_000289"
     CDS             22972..23550
                     /gene="pth"
                     /locus_tag="WIE22_000289"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ4126436.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            23611..23817
                     /locus_tag="WIE22_000290"
     CDS             23611..23817
                     /locus_tag="WIE22_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ4126437.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            23814..24176
                     /locus_tag="WIE22_000291"
     CDS             23814..24176
                     /locus_tag="WIE22_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ4126438.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            24407..25822
                     /locus_tag="WIE22_000292"
     CDS             24407..25822
                     /locus_tag="WIE22_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ4126439.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            25941..26450
                     /locus_tag="WIE22_000293"
     CDS             25941..26450
                     /locus_tag="WIE22_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ4126440.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKNGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            26617..27999
                     /locus_tag="WIE22_000294"
     CDS             26617..27999
                     /locus_tag="WIE22_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ4126441.1"
                     /translation="MASIARDIFKAYDIRGIVGKTLTDEAAYLIGKAIAAKAAEKGIT
                     RIALGRDGRLSGPELMEHIRRGFTDSGINVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYLKHI
                     TGHIRLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(28019..28774)
                     /locus_tag="WIE22_000295"
     CDS             complement(28019..28774)
                     /locus_tag="WIE22_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ4126442.1"
                     /translation="MIKIRNIHKTFGENTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(28784..29500)
                     /locus_tag="WIE22_000296"
     CDS             complement(28784..29500)
                     /locus_tag="WIE22_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ4126443.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(29490..30317)
                     /locus_tag="WIE22_000297"
     CDS             complement(29490..30317)
                     /locus_tag="WIE22_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4126444.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            30602..31510
                     /locus_tag="WIE22_000298"
     CDS             30602..31510
                     /locus_tag="WIE22_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ4126445.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLQHLSAAVSGGYYYAPSVERLQNL"
     gene            31743..>32613
                     /locus_tag="WIE22_000299"
     CDS             31743..>32613
                     /locus_tag="WIE22_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEZ4126446.1"
                     /translation="MSAPIQAFDSLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDGALSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPF"
BASE COUNT         7854 a         9349 c         8059 g         7351 t
ORIGIN      
        1 gtaaattcaa accgtaggtc gggcattgat gtccgaccta cgcggactca agcctccgac
       61 aaactgtttc ggataaattt gaaccggtac atcagccgtc attcccgcgc aggcgggaat
      121 ccattgataa aactcagttc atcagattta aacagcgatt gcccaaattc agaaatggat
      181 tcccgcctgc gcgggaatga cgccaaatga ttatttttcg gttgcaacac gctgtttatc
      241 aaaaacaggt cgtctgaaaa cctgattttc tgttttcaga gtgcgaagcc tgcccgtggg
      301 tacgaccttt tcgatttatt tgcccaatgc gcccaatact tcggctttca cggcttctac
      361 cggctgagtg ccgtcaactt tgatgtattt aggcgcgtgt tcgccttcca gtttgctgta
      421 aaaatcgacc aaaacttcgg tttgctcgtg gtaaacggca aggcgttttt tcacggtttc
      481 ttctttgtcg tcgtcgcgct gaatcaaatc ttcgccggtt acgtcgtctt tgccttcaac
      541 tttgggcggg ttgtaggtaa cgtggtaagt acggccggaa gccaaatgca cgcggcggcc
      601 gctcatgcgg tcgacaatca cgctgtcagg cacatcgatt tcaacgactg catccaaatc
      661 cacgcctgct tcaaccatcg cttcggcttg tgccaatgtg cgcgggaaac cgtcaaacag
      721 gaaaccgttt ttgcagtcgt cttgcgcgat gcgttctttg accatgccga taatgatgtc
      781 gtcgcgcacc aagccgcctt cgtcaatgat ttttttcgct tccaaaccca agggcgtgcc
      841 tgccttaatc gcggcacgga gcatgtcgcc ggtagagatt tgcggaatgc cgaacgctgc
      901 ggtgatgaat tgcgcctgag tgcctttgcc cgcgcccggt gcgcctaaaa gtaatgcttt
      961 catgaagtga tccttttaaa tgtgtttatt ggaatcaaac agatggttta cgattctaca
     1021 cggcttggca caatagttca aaaaccaact gcacaaactg ccatttgcaa gcaaatgttt
     1081 tgcatacctt tgccgagcct gatattcaca aggcggcaac tttttgggac aatccccaaa
     1141 atttcaaaac atcacaccct accaaaaagg aacatccgtg aaacgcattt ttctgttttt
     1201 ggctaccaat atcgctgttt tggtcgtaat caacattgtt ttggcggttc tgggcatcaa
     1261 cagccggggc ggcgcgggca gcctgttggc gtattccgcc gtcgtcggct tcagcggctc
     1321 gattatttcg ctgctgatgt ccaaattcat cgccaaacaa tcggtcggtg cggaagtcat
     1381 cgacacgccg cgcaccgaag aagaagcctg gcttttgaac actgtcgaag cccaagcgcg
     1441 gcaatggaac ctgaaaacgc ccgaagtcgc catctaccac tcccccgaac ccaatgcctt
     1501 tgccacgggc gcatcgagaa acagctccct gatcgccgtc agcaccggtt tgctcgacca
     1561 tatgacgcgt gacgaagtgg aagccgtatt ggcgcacgaa atggcacacg tcggcaacgg
     1621 cgatatggtt acgctgacgc tgattcaagg cgtggtcaat acctttgtcg tgttcctgtc
     1681 gcgcattatt gccaacctga ttgcccgaaa caacgacggc agccagtccc agggaactta
     1741 tttcctggtc agcatggtat tccaaatcct gttcggcttc cttgccagct taattgtcat
     1801 gtggttcagc cgacaacgcg aataccgcgc cgacgcgggc gcggcaaaac tggtcggcgc
     1861 gccgaaaatg atttccgccc tgcaaaggct taaaggcaac ccggtcgatt tgcccgaaga
     1921 aatgaacgca atgggcatcg ccggagatac gcgcgactcc ctgctcagca cccacccttc
     1981 gctggacaac cgaatcgccc gcctcaaatc gctttaaacc gatttgaaac ggcaaaaacc
     2041 gtacgcgcaa gcagtacggt tttttgtata tgctgtctga aaacatcagg tttccatacc
     2101 gacaccaacc gtatccattt ccgcaccgga tacgccgcat tgatataccc tgataaaccg
     2161 tatccggaca gaaaatatag tggattaaca aaaaccagta cggcgttgcc tcgccttagc
     2221 tcaaagaaaa cgattctcta aggtgctgaa gcaccaagtg aatcggttcc gtactattta
     2281 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atcccctata tcagataaaa
     2341 cagcggtaaa attgccgtct gaaaccaact ctgcgccata cgggacacgc tctgcacgtc
     2401 tatttgcctc cgatatatcc tccctactgt aggaacgtct gccgatttgc acgaaatcgg
     2461 cacctctagc acccttcagg atggcggacg ttatccgttc ttgggattct gcaagacggt
     2521 cgacggcatt atacagacgc tcggtctgat acccttccga agcaacctgc ttcaggtatt
     2581 ccaaataacc gtaacccaac gcccctccga caagcagggt cgtcaacgct accagcgttg
     2641 ccgaacggat tttagggggc atatcttgaa gcatatctct accgaaccga accaaagtct
     2701 ctttaaagtc ggactctaca cgcgtagaac cctcggaaat cttgaagttc aactcatact
     2761 gcttccgttt gtccgctgtc aaccgcttga ggttgtcatc cccgtattgg acggttgaca
     2821 caaccctgta cagactgtcc tgtatttcga ccagcgtctg tgcaaagttg gcggtcataa
     2881 ctggattccc aacccttgcc gccgattctg atactcaaaa cggtatccag gcgttccagc
     2941 agggcttcgg gagacagcag tgcgtattct tcaatatttt ccataggttg attatagcag
     3001 aaccttatac ctgttacaga caaggttgtc gggatttgaa cgtccgtcga gcttggttcg
     3061 tatagagccg tagggggtgt ttagggcttt ggcggcagct tgtacgctgg ggaacgtgag
     3121 tgtttcgcct gtggcggtgt cgaggatgga gacaggtttt tggttttggg gaggcgtatt
     3181 ttccttcatt ttaagtttcc cgcactccgg acacccatac tttgacttta tagctgtgtc
     3241 gtaatacaaa agttggacaa ccccgtgcgt aggacatcta acactggaag gtttataaat
     3301 gccactgaac tccaacaatt ctaaatgtgg aaatttctct tgaaagcgtg atttggcggt
     3361 atcgaaagtc attcgcttac ccataatttg tctccatagg ttatgtgcaa atggattaca
     3421 caaccaaaat atagaaattt tcaaattttt tgagacctac ctgaaatcct gcgcaagcac
     3481 aatgtaaaga aaaatggtat acgtctttac taaatcttta cataaattaa ctctaatcgg
     3541 gatttgaacg tccgtcgagc ttggttcgta ggatttaggg ggttgtgaac cttacctatc
     3601 gtacccaagc actttgtacc tgtttgaaat aaggttgtcg ggcgacgttc gacccttgag
     3661 cctgtgattg atttgttgga aatgtacacc taaagcagca cctgcggcag taacgcttgg
     3721 gaacgtgagt gtttcgttgg tcgttgtgtc gaggatgagg aggggcttcc ctacatttgg
     3781 aggtgtttgg gtttttgaca tcttagcaca ctcaggacac ccgtactttg actttataag
     3841 ggcattaaat gtcgaataac gaacgttccc gtgcatagga caattaacgg ttactgggta
     3901 attcacaccg tcaaagccgg ttagagagat attagggaat atatcagtaa gttcagaagc
     3961 tgcttggttg aaagatttgg ccatttgaga ctccaaaatt gattgtcaaa tggatttaat
     4021 agcaaattta taaaaatgtc aaatttttcg agaagcacct gatagtggcg aggggtatac
     4081 actgttaaaa agtgtatacg tcatttagtg cagatgtgaa ccttatacct ccctgcaagt
     4141 aaagtctctg tgtgaacccg accttttata cggtcagtga tagctgctgg gtttataccc
     4201 aatgaccttg cagcagatgc tcttgagggg aatgtaagtg tttcgccagt tacagtatcc
     4261 tccaatactg tatttttaga taatttatct attgtatctt ctggaatttt gtgggaattg
     4321 acaccataag atgcacattt tggacaccca cttttagagt ccaacatact tttgaatgta
     4381 gagcaagtaa ccctcccgtg tataggacag acgattaccg tcgggtaacg aacgccattg
     4441 aactccacaa gattcaaatg tggaaattta gaggttagct tagaaactgc ttgagtaaat
     4501 gtaagtgcca tagtggttgt gaacttactt taccaaccta taacgatacc taatcaatcg
     4561 gtcaaacgga gtgcgtccgc ttaaacgtac agctaaagtg ctgttggagc aaccgataaa
     4621 tttactggca gccagtgtac ttgcaaactc gtagatttcc cctgtctctg tgtcctccaa
     4681 cctaatggca acggcgtttg gagagggctt ttcgggattt cgagtacatt caggacatcc
     4741 tgttgaggat ttcatggcag cttgtagatt cccgtaaaca accgacccat gcaaagggca
     4801 ttggattgtt accggttcgt agtttttagt gaacttcaaa accgtcttat caggaaattt
     4861 actggaaaac cttaactgaa catcttgtag cgagagtgat tttggcatta tagacctttt
     4921 taaaatgaaa gcattataca accaaaatat agaaattttc aaattttctc tgaacgcact
     4981 ggtatccgat ggagctaaat gtaaagaaaa agggtatacg tctttactaa atatttacac
     5041 attttaactg tatagtgttt gacaaacaac aggcataggc gcataatgca cccgtagtta
     5101 gatagatagt aaggagataa ccaatgactt agcacctgcc ccctctctct cgcgcacccc
     5161 gcccctcgct tgcggctaat gggctttgcg cgacatatac actttcatct cctgcatggc
     5221 ttaaccgcct atgcaatagt acacacatca acaaatcagg ctggcacgtc ttggcattgg
     5281 ctgacgtacc attgtagccc gaaatgttga taccctacta tttatgacca acggcggatt
     5341 agggcttcag acggcaattt tactgcaaaa ccttatgcga acggatgatg cagcacaatc
     5401 gtttcttctc ggtcggggcc ggtggagacg atagcgaccg gcgcgccgca gacttcttca
     5461 atccgtttca aatatgcttt ggcgttttcg ggcaatgcgc cgtagtcctt cacgccgaaa
     5521 gtggattcac gccagccggg catggtttcg taaatcggct tgcaggcttc taccgcatca
     5581 gagccgcaag gcaggatgtc ggttttgccg ccgtcgggca gttcgtagcc gacgcagata
     5641 ttgatggttt caacgccgtc cattacatcg agtttggtaa tgcacatacc ggaaatgccg
     5701 ttgatttgga tggagcgttt cagggcggcg gcatcaaacc agccgcagcg gcgcgcacga
     5761 ccggttacag aaccgaattc gtgtccgcgc tccgccaaac ctgcgcctac ttcgtcgaac
     5821 aattcggtcg ggaacgggcc cgaaccgacg cgcgtggtat aggctttgac gatgcccaaa
     5881 acataatcca gcatttgagg gcctacgccc gcgcctgccg aagccgcgcc ggcgaggcag
     5941 ttggacgagg taacgaaggg ataagtgccg tagtcgatgt ccaacaacgc accttgcgcg
     6001 ccttcaaaca gcagtttttc gccgtttttg tttttctcgt tcaacacgcg ggacacgtcg
     6061 gtaatcatcg gcgcaatgcg cggcgcgact ttttcgataa ccgccatcac gtcttccgct
     6121 ttaaccggtt cggcattgtg cagatgttgc agttggacgt tgtaataagc aaggacgaca
     6181 tccagttttt cacgcagttt ttcaggatgc agcaaatcgg cggcgcgaat ggcgcggcgt
     6241 gccactttgt cttcgtaggc agggccgatg ccgcggccgg tcgtgccgat tttgcctttg
     6301 ccgcgcgatg cttcgcgggc ttggtcgagc gcgatgtgat aaggcaggat cagcgggcag
     6361 gtcggcgcga ttttcagacg gccttcgacg tttttcacgc ctgccgcgtt caactcgtcg
     6421 atttcgccca acagggcttc gggggagacg acaacgcccg aaccgatgaa gcagtccaaa
     6481 ctttcatgca ggatgccgct cggaatcagg cgcaaaatgg tttttttgcc gccgacaacc
     6541 aaggtatggc ccgcattgtg gccgccttgg aagcgcacca cgccgccggc ttcttccgcc
     6601 agccagtcaa cgattttacc tttaccctcg tcgccccact gtgcgccgat tactacaaca
     6661 tttttagcca tagccatata acctatcgat attaaaaatt attgcggaaa tccccaaacc
     6721 ggtgcggctt tgcgccccgc cacgatttgc ctttcatccg ccatgaacgg gtatttagcg
     6781 tttcacgacc tgccaaacgc cgtccgtctt tttcagacgg cctgcaagct cttccgaaac
     6841 attgtgtccg ataccgtagt caatcacgac acactgccct tgttcacgca aggcttcgac
     6901 tgcttcgcgc gccgcttcgg catcttccgc atcgaccaac acggcgggct gccgttcgat
     6961 ggtgggcaaa cgcccgataa agctgcgcaa gtcgaaactg aatcccgttg ccgggcgcgc
     7021 cctgccgaaa tatccgccca atccgtcata acgcccgccg cgcgcgaccg cgtcgtggaa
     7081 atcggcggca taggcggcat acagcaagcc cgtgtggtaa ttgtcgacac gcagctcgga
     7141 caagtcgata tggatttcac aatcggggaa tgcgtcgcac accgcctgca attcgcccaa
     7201 cgcgccgccg accgccgaca aatccggcaa ccgtccgcgc gcgtcggaca acacttcacg
     7261 cccgccgtac aggcgcggca gcagcgagaa tgcttttgcc cacataccgt ccagcttcca
     7321 agccttgacc tgcgcttcga ccgtctcggt atctttatcc tgcatcaagg caagcagcgt
     7381 tgcagactgc cccgcatcca aatgcgccgc atcggacaag gcgcgaaata tgccgatatg
     7441 ccccagcgaa agcagcactt tgcccatatc ggcaattttc atgcttttca gcatcaggtc
     7501 tatcagctcg atgtcgccac ggatgtcggc gaaaccgtac atttccgccc ctgcctgcaa
     7561 gggttcgcgc atattcagca gaccgtcgga ctgcgcgtgc aacaccggac cggcataaca
     7621 caaccggtta atcccttggt tggcggataa aagatgggca tcgatacgcg ccacctgcgg
     7681 cgtgatgtcg gcgcgtatgc ccaactgcct gccgctgagc ctgtccgtta ccaaaatggt
     7741 tttcagggaa agccccgcat cgatatgcgt cagcagggaa tgtgcgtact ccatcagcgg
     7801 aggctgtacc agttcataac cgtgtacgcg gaacagtgcc aacaactgct ccctcgcgct
     7861 ttcaagctgc cgcgcgttcg tgggcagtac gtcggcgata tgttccggaa gctgccatgt
     7921 ctgcatgaga atctgccttc tattttatca tgtaaataca aaaggctgcc cgatccgcaa
     7981 tcagacggca tctttgccgc aacgcatcac gccgctgtca aaacggcgat gcttccttcc
     8041 gattcagaat ccgaacagcc cctgtccgac ggctcaatgc cgtcaagtcg ttaaaaccaa
     8101 actttaccat aaaatacaca caatctgaat atacttttat accctgtatc cgccgtctga
     8161 cggcacggca tttttttact atataatgtg cgccattatt tctcatcact tcctgctgcc
     8221 gttttctgct tcagacggca tttttgttga ttgaacactt atgctgctcg acctcaaccg
     8281 cttttccttt tccgtcttcc tgaaagaagt ccgcctgctg accgctcttg ccctgcccat
     8341 gctgttggcg caggtcgcgc aggtgggcat cggttttgtc gataccgtga tggcgggcgg
     8401 tgcgggcaag gaagacttgg cggcggtggc tttgggcagc agcgcgtttg ccacggttta
     8461 tattaccttt atgggcatta tggcggcgct gaacccgatg attgcccagc tttacggcgc
     8521 gggtaaaacc gacgaagtgg gcgaaacggg acggcagggg atttggttcg ggctgttttt
     8581 gggcgtgttc ggcatggtct tgatgtgggc ggcgattacg ccgttccgca actggctgac
     8641 cttgagcgat tatgtggaag gcacaatggc gcagtatatg ctgttcacca gcttggcgat
     8701 gccggcggca atggtacacc gcgcactgca cgcctacgcc tccagcctga accgcccgcg
     8761 cctgattatg ttggtcagct ttgcggcgtt tgtgttgaac gtgccgctga actatatttt
     8821 cgtttacggc aaattcggta tgcccgcttt gggcggcgca ggctgcggac tggcgacgat
     8881 ggcggtgttt tggttcagcg cgctggcatt gtggatttat atcgccaagg aaaatttctt
     8941 ccgcccattc ggactgacgg cgaaattcgg caaaccggat tgggcggtgt tcaaacagat
     9001 ttggaaaatc ggcgcaccca tcgggctgtc ttattttttg gaagccagcg cgttttcgtt
     9061 tatcgtgttt ttgattgcgc ctttcggcga ggattatgtg gcggcgcagc aggtcggcat
     9121 cagtttgtcg gggattctct atatgattcc gcaaagcgtc ggctcggcgg ggacggtgcg
     9181 catcggcttt tcgcttgggc ggcgcgaatt ttcgcgggcg cgttatattt cgggcgtgtc
     9241 actggtgtta ggatggatgc tcgccgtgat taccgtgctt tccttggtat tattccgttc
     9301 gccgctggta agtatgtaca acaatgatcc ggcggtttta agcatcgccg ccaccgtctt
     9361 actgttcgcc ggcttgttcc aaccggcaga cttcacccaa tgtatcgcct cctacgcctt
     9421 gcgcggctac aaagttacaa aggtgccgat gttcatccac gccgccgcct tttggggctg
     9481 cggtctgctg ccgggctacc tgctcgccta ccgtttcgat atgggcattt acggcttctg
     9541 gacggcattg attgcctcgc tcaccatcgc cgccatcgcc ttggtgtggt gcttggaatt
     9601 gtgcagtagg gagatggtca gatcgcataa ggccgtctga aaactccgcc gattaaaaat
     9661 atagtggatt aacaaaaacc agtacggcgt tacctcgcct tgccgtacta tttgtactgt
     9721 ctgcggcttc gttgccttgt cctgattttt gttaatccac tatagaaacg atgggctgaa
     9781 gcccgccact ggcaatactc attttaaagt tcaaacccat gcaacccatc cgataccgaa
     9841 ccgaccttac cccctacaac actttcggcc tgcgcgccca agcccgagcc tttattgcgc
     9901 ttgaacacgc cgacgggttg cgcgacatcg tccggctacc cgagttcaac cgagatactg
     9961 ttttatggct gggcggcggc agcaatatcc ttttgatgga ggactacgcc ggtctggtcg
    10021 tacatatgga aaacaaaggc atacgcgaaa ttgctcgttc agacggcatg gttctgattg
    10081 aagcgcaggc gggcgaaatc tggcacgatt ttgtcctgca cgccgtcgcg ctgggtttga
    10141 acggtttgga aaacctgagc ctgattccgg gtacggtcgg cgcgtcgccc gtgcagaaca
    10201 tcggcgcata cggcgtagag gcgaaagatg tgattcacag cgtgcgctgc ttcgatttgg
    10261 atacggaaac ctttgtaacc ctttccaatg ccgactgccg cttcgcctac cgcgaaagcc
    10321 tgttcaagca ggaaggtaaa gggcgttatg tgattgtttc ggtcgtattt gcattaaaaa
    10381 cgcattttgt gccgaacttg ggttacggcg atttggcggc cgccgttgcc gaactgagcg
    10441 cgggcagggt gccgacggcg aaagatgttt ccgatgcagt gtgtgcaatc cgcaacagta
    10501 aacttcctaa tcctaacgtg cttggtaatg tcggcagttt ctttaaaaat cccgtcatcg
    10561 gcgcggaaaa agctgccgcc ctgttggagc agcatcccga tatgccgcat tatccgcagc
    10621 cggacggttc ggtcaaactc gccgccggct ggctgatcga ccaatgtcgt ctgaaaggct
    10681 tccaaatcgg cggcgcggcg gtacacgaca aacaggcatt ggttttggta aacaaaaaca
    10741 acgcctcggc aaacgatgtc cggcagttgg cgcaacacat caaatttaca gtatttgctc
    10801 ggtttcaggt agaattgcac gccgaaccca attggctgcc tgcttcgttc agcctgtaaa
    10861 tccaaggagt atgcccgtga cccgccccgc caaaatcaat acttacacac gcatcatcga
    10921 cgccagcctt gcgcttttca acgaggaagg cgagcgcaac atcagtacca accatatcgc
    10981 ggcgcatttg ggcatcagtc cgggcaatct ctattaccac ttccgcaaca aagacgaaat
    11041 catcgtccaa ctgttcaaac gttacagcga agccctgctt gcctacctca acgaagccgt
    11101 gttgccgtct gatgtggaag actccatcaa ctatatggcg ggcatttacg acgtgatgtg
    11161 ggaataccgc ttcctattca gcgacgtaaa caccctgctt gcacgcagtg ccgaattgtt
    11221 gggcgaacac aacaccttca cccaagccaa agtctccccg ctcttggtca acctgctgac
    11281 ccagctcaac ggtctgaaca tcatccaagc cgaccaaacc gctatgaacg atctcgccgt
    11341 caatatgtgg atggttacga aatactggtt cgacttcgac agctccctgc gcggccgcac
    11401 caagctgacc gaagactcca aagcacgcgg catccgccgc accttaagcc tccttcgtcc
    11461 ttatcttttg cccgaacacc gcaagaaata cgaccggaaa atcggcaacg gcaacccgta
    11521 aacccacagc gtccgaatca gccttcagac ggcacttacc cgacggtatc agcgaatgcg
    11581 cgcagtcccc ccgccccata tctccatcac aggcctaggt tacctcggcc tgccgctggc
    11641 acaaaagttt taccaacacg gcagccgcgt cgccgccata aaacgcagcc tgacttcgga
    11701 cgacatcaac ctgcccatac atctcgacac cttcgacctc ggcagcaccg atgcctttca
    11761 gacggcattg tggcggcacc acgccgacaa acccgtctgg ttcttcctgc taccgccctc
    11821 ctcgctgacg cattacgccg acaccgtcaa acaatgggca gaacttgccc gggcgtgcaa
    11881 cgtgcaacac ctgattttca caagcagcac cagcgtttac ggcgatacag cgcgcgaatg
    11941 cgacgaaacc gccacacccg atccgcaaac cgagtccgcc cgccaaatcc tcgccgccga
    12001 acaacacctg ctcgacagcg gcatctccaa catcgacatc ctgcggctgg gcgggcttta
    12061 ttgcgccgaa cgccatcccg tcagccgcct tgttcaaaag caaaacatcc cgggcggcaa
    12121 ccgccccatc aacatcgtcc accgtaatat cgccgtcgaa agcctgtttc agacggcatt
    12181 taaccccggc ggcaggcggc tgaaaaacat tatcgaaccg cgccacccga cacgacgcga
    12241 attctacacg gaagaagccg ccaaactcgg tttcccggct cctgatttcg cacccgacga
    12301 cagcatgggc aaaatcatcc gtaccgtttg cgataacagc tgaagcctgt aaaataagcg
    12361 tcaacagcaa caaacaggca ttctccgccc cgatcagaaa atatgtccgc cctcctcccc
    12421 atcatcaacc gcctgattct gcaaagcccg gacagccgct cggaacttgc cgcctttgca
    12481 ggcaaaacac tgaccctgaa cattgccggg ctgaaactgg cgggacgcat cacggaagac
    12541 ggtttgctct cggcgggaaa cggctttgca gacaccgaaa ttaccttccg caacagcgcg
    12601 gtacagaaaa tcctccaagg cggcgaaccc ggggcgggcg acatcgggct cgaaggcgac
    12661 ctcatcctcg gcatcgcggt actgtccctg ctcggcagcc tgcgttcccg cgcatcggac
    12721 gaattggcac ggattttcgg cacgcaggca gacatcggca gccgtgccgc cgacatcgga
    12781 cacggcatca aacaaatcgg caggaacatc gccgaacaaa tcggcggatt ttcccgcgaa
    12841 tccgagtccg caaacatcgg caacgaagcc cttgccgact gcctcgacga aataagcaga
    12901 ctgcgcgacg gcgtggaacg cctcaacgaa cgcctcgacc ggctcgaacg cgacatttgg
    12961 atagactaac cttcagacgg catccgacat gaacagccct tttcacaaca tcggcatcgt
    13021 aacccgcccc aacacgcccg acatccaaga caccgcacac acgctgatta cctttttgaa
    13081 gcagcacggc tttaccgtct atctcgacga agtcggcata aaggaaggct gcatctatac
    13141 ccaagacacc gtcggctgcc atatcgtcaa caagaccgaa ctggggcaat actgcgacct
    13201 ggtcgccgtt ttaggcggag acggcacctt tctctccgtc gcccgcgaaa tcgccctgcg
    13261 cgccgttccg attatcggca tcaaccaagg gcatttgggc ttcctgaccc aaattccccg
    13321 cgaatatatg acggacaagc tattgcccgt tttagaaggg aaataccttg ccgaagagcg
    13381 catcctgatt gaggccgcac tcatccgcga aggcaaaacc gccgaacgcg ccatcgccct
    13441 caacgatgcc gtcctctccc gtggcggtgc cggacagatg attgagtttg aagtcttcgt
    13501 caatcgggaa ttcgtctata cccagcgttc ggacgggctg attgtctcca cccccaccgg
    13561 atcgaccgcc tattcgcttg ccgccggcgg ccccatcatg caggcaggat tgcacgcctt
    13621 cacgctcgtc cccatctgcc cgcaatccat gaccaaccgc cccatcgcca ttccagacac
    13681 gtccgaaatc gaaatcctcg ttacccaagg cggcgacgcg cgcgtccatt tcgacggtca
    13741 aacccatatc gacgtgcaaa acctcgaccg catcaccatc cgccgctacc gaaacccctt
    13801 acgcatccta catccgaccg actaccaata tttcaaaacc ctgcgccaaa aactgcactg
    13861 gggcgaacaa ttggtctgaa ccgaccctta ccggaaaaca cctatgaatc caaaaactcc
    13921 ccccgaccat acagaagatg ccgtgcgcct gtccaaacgc atggcgcaac tggggctttg
    13981 ttcgcgccgc gaagccgacg gctatatcga acagggttgg gtaacggtca acggcaaaac
    14041 cgccgtactc ggtcagaaag tttcgccggc agaccgtatc gaactaaaca agaaagccca
    14101 cgaacagcag gcggcacgca ttaccatcct gctgaacaaa cccgtcggct atgtcagcgc
    14161 acaagcggaa aaaggctata aatccgccgc cgaactgatt acctctgaaa accgctggga
    14221 aagtgacacc agccgcatcc gtttcgatcc gaaacacaaa atcggtcttg cccccgccgg
    14281 caggctcgat atcgattcag tcggattact ggtgttgacg caggacggcc gtatcgccaa
    14341 gcagcttatc ggcgaaaaca gcggcagtga aaaagaatat ttggtgcgcg tgcgcggaaa
    14401 attggacgaa aaaggacttg ccttactgaa tcacggattg agtttggacg gtgagaaact
    14461 gcgtcccgcc caagtagaat ggcaaaacga agaccaactg cgcttcgtgt tgaaacaggg
    14521 caaaaagcgg caaatccgcc gtatgtgcga actggtcgga ctgcgcgtcg tcgggctgaa
    14581 acgcatccgc atgggtaagg tcaagctcgg caggctgccg cccggcaaat ggcgttatct
    14641 cgcccccggt gaatcgtttt aaataagcat taaaaaatgc cgtctgaaaa tccttgcgtt
    14701 ttcagacggc attgccttat gcccaaataa cggtttcttc caagggcctg cgcgccttcg
    14761 tggcgatttc ctgaacgcgg taaccgaatg tcgcggcgac ggacacgccc cattctgccg
    14821 catcgaacaa accgaactgc ccggacaata cgcgttccat atcggcatag ttgaaacctt
    14881 ccaccgggca ggaatcgata cctgccatcg ccgcacccgt catcatgttg cccaacgcga
    14941 tataggtctg gcggcaacac cagtcaaaca aggcgcgcga atcgtccaaa atcttgatgt
    15001 cgtcggcttg aaacgcctga tacctcgcca aagattttgc catggcatcc ggttcggtaa
    15061 cgccgcgccg tttgaggctt tccaacataa acgggctgtc gaagcgggca tttttctttg
    15121 ccaaaaacac caccaaatga ctggcggtat ccaaagcatc cgccataccc caagaaaacg
    15181 gcttgattgc ctgtcggatt tcggggtttt gaaccacaac aaactgccaa ggctccgaac
    15241 cgaccgaact gggcgacaaa cgcccgagtt ctaaaataaa ctgaaaatcc tcggcactga
    15301 ttttgcgtgc cgcatcgtaa tgccggcatg atttacggtt tttaaatgcg gatagaacct
    15361 gctctctgtc taataccgtc atagtgcatc cttttattac tggtattgca aattgatttc
    15421 aaaccgatac ggcactgccg caccatacaa tccgtcctcg ccatcaagct tcatccggac
    15481 tttacccatc tctctacggc tgggaaaaca aagcggcggc agactcggga ataacgcccc
    15541 aatgcctttc agacggcatt tgggcatcat tgcaaacgcg cctacgggta ggcgatatag
    15601 gcataagccg aatggttgtg gatagactcg aaattctcac tctcgacgac gaaactcttg
    15661 atgcgtttgt cggcgatcag cgaagtagcg acatcacgca ccatatcttc cacgaatttc
    15721 gggttttcgt aggctttttc ggtaacatat ttttcatcgg ggcgtttgag caaaccgtag
    15781 agttggcagc ttgcctgcgc ctccacataa tcaatgactt cctcgatacc gacttctgca
    15841 tcggcagtca ggctgacggt aacatgcgaa cgctggttgt gcgcgccata ttgggaaatt
    15901 tctttggaac acgggcaaag cgaggttacg ggaatcatga ccttcatact gtagccgtat
    15961 gccccgtctt tcatttcgcc cgtgagacag acatcataat ccagtaagga ccgaataccg
    16021 gaaaccggcg cggttttctt gcggaaaaac ggaaaagaaa cgctgatttt gccggcgcgg
    16081 gaatctaaaa gcgcgaccat ttcggcagtc agcctgtgca attgtgcaaa atccaaggct
    16141 tcagaatgtt gctccatcaa tgcgacaaaa cgcgacatat gcgtcccctt ctgctcagca
    16201 ggcaggtaaa ccgtcatggt cagacgggca acggtggact ggatgccttc tgcagtttgc
    16261 agggtaatcg gaaagcgcag gtctttgata ccgacctgat tgatcggcag attgcgcaaa
    16321 tctcggctgg attgcacgtc tgcaatagtg ttcatgagtt gtttttcctt aatattcatg
    16381 taattgttga gatgtgtgca tggtatggcg tttccgccgg ggaatatacc gtcaatctgc
    16441 aaatgcgaga ttatatcact cgcttttagc agctgcattg attgtttcta tttttcagat
    16501 agtgataaaa tcttcgatta ttcacatcta cataggcaga gaccaaatga aattcgccac
    16561 gaaagccatt cattccagct acgattgcga cgaacacaac cgcgcgctga tgccgccgat
    16621 ttatcaaaac agtatgttta cgttgcacga gattggcgaa aatgtgcctt accgttattc
    16681 gcgcctgagc aacccgaccc gtcagatttt ggaagacacc gttgccgatt tggaacacgg
    16741 tgcggcaggc tttgcgtttt ccagcggtat ggcggggatt gatgccgtat ggcgtacctt
    16801 cctgcaaccg ggcgatacca tcgtcgcggt cgccgatatt tacggcggcg cgtatgactt
    16861 attggtcgat gtttatcaaa aatggggggt aaatgttgtt tttgccgatt taggcaatcc
    16921 cgacaatttg gacgaattgc tcaaagcgca caaggtcaaa ctggtttggc tggaaacgcc
    16981 gtccaatccg cttttacgct tggtagacat caaagccctt gccgcaaaag ccaaagcgac
    17041 cggcgcgctg gtcggcatcg acaacacctt tgccacgccg tatctgcaac agccgttgga
    17101 tatgggttgc gattttgtgt tccattccgc taccaaatat ttgtgcggcc attccgacgt
    17161 attgatgggc atcgtcgttg ccaaaaccaa agagctggct cagcctttgc acgatatgat
    17221 ggtgcatacc ggtgcgattg caggcccaat ggactgctgg ctggtgttgc gcggcatcaa
    17281 aacgcttgcc ctgcgtatgg aggcacactg caaaaacgca ctcgaaatcg cgcgccgtct
    17341 ggaagaccat cctgccattg aaaaagtgtt ccatcccggc ctgccgtcgc acgaacatta
    17401 tgaactcgcc caagcgcaaa tgcctaaagg catcggcggc gtggtgacgg tttatctcaa
    17461 aaacgacacg cgcgaagcgg caaacagcgt gattaaaaac atgaaactgg tcaaaatggc
    17521 ttccagcctc ggcggcgtgg aaagtttggt caaccattgc tattcccagt cccacagcgg
    17581 cgtgccgcat aatgtgaaaa tggaaatggg catcaaagtc ggcctgctgc gtttctccat
    17641 cggcatcgaa gacgcggacg atatttggaa cgatatttcc gccgcactcg atacgacttt
    17701 gtaaactgta aaaatgccgt ctgaaaccat ggtttcagac ggcatttcaa ttaacccggc
    17761 cgaaaatcaa cgcttcaaca tctttgccgc ctcaatagcg taataggtca aaatcccgtc
    17821 cgcacccgca cgtttgaatg ccagcaggct ttccaaaacc actttgccgc cgtccagcca
    17881 gccgttggca atcgctgcct gcaacatcgc gtattctccc gaaacctgat aggcataagt
    17941 cggtacgccg aactcgtcct tcacgcggcg gacaacgtcc aaatacggta aaccgggctt
    18001 caccatcacc atatccgcac cttcccgaat atcgagcgcc acttcattca gcgcctcatc
    18061 ggtatttgcc ggatccatct ggtaggtttt cttgtccgcc ttgcccaaat tgcccgaact
    18121 gcccaccgca tcgcggaacg gcccgtaaaa cgccgaagca tatttggcgg aatacgccat
    18181 aatccgcgtg tggatatgcc cggcatcctc caacgcctcg cgaatcgcac cgatacgccc
    18241 gtccatcata tcggaagggg caaccacctg tgcgcccgct tcagcgtggc acaaagcctg
    18301 cttgaccaaa acctctacgg tttcatcgtt catcacataa ccgttttcgt ccgtcagccc
    18361 gtcctgaccg tgaaccgtat aaggatcgag cgcgacatcc gtcataatgc ccagttcggg
    18421 aaacctctcg cgcagggcgc ggacggctgc cggcacgagt ccttcgggat tgtacgcttc
    18481 ctctgcacgc tcggttttgt ttgccgtaac cacgggaaag agtgccaaca tcggaatacc
    18541 gagctttacc gcctcttccg ccgtaaacag cagcctgtcc aaactttgac gcttcacacc
    18601 cggcatagaa ggcacatcct cctcgcgcgc cgacccctcc aatacgaaca ccggataaat
    18661 caaatcatcg gcggtcagcg tgtgttcgcg catcaggcgg cgtgaaaaat cgtccctgcg
    18721 catacggcgc atacgcgaag ccggaacatt gcggtaagga aactgcataa gccctccaat
    18781 catcctgtca acaattcaaa ccatacggaa gccgccgcaa cttgcagaca gcatcgggcg
    18841 gcaatcaaaa tccgttttca ccggaaaggg tttcggcccg ccgaaatgcc gcgccgaagg
    18901 acggcgtccc gcccattaaa gaacaatagc aaaacaacag cttcaggctg tcagccttcg
    18961 atattcttac gccacaatac caaaagtttc ccgatatgct gaacaggttg cgcgtcaacc
    19021 gcctcacata aggcagtgca gatttcgata cgttcggcac ggtcgtcgcc gaatacgcgc
    19081 actttaatca gctcatgcgc cgtcaatgcc gcatcggttt ccttgatgac cgcgtccgtc
    19141 agaccctgct gaccgaccat cacaacagga tggagatggt gcgcgcgcgc tttcagttcc
    19201 aaaatttctt tggtgttcaa tttggtatcc gtcattttcc tactgcttga ataaaaagaa
    19261 taatgcggca ttgtacgcga tttggcacga catcggcaaa attaacgtac aatacgcggt
    19321 ttccattccg acccgcgaac cactatggct gtacgttcca aatcctcaaa agcgtggctg
    19381 cacgaacacg tcaacgacca ctacgtccat atggcgcaaa aagacggcta ccgcgcccgt
    19441 gccgcataca aacttttgga aatcaacgac aaagacaaat taatcaaacc cggcacggta
    19501 cttgccgact tgggcagcgc gccgggaagc tggtcgcagg ttgccgccaa gctgacgggt
    19561 tcttccggag cagttttcgc cttggacatc ctgcctatgg aagccatagg gggcgtctcc
    19621 ttcattcagg gcgacttccg cgagaacgac gtactggcac aattcgaaac cttgttggac
    19681 aaccgcccgc tcgaccttgt aatttgcgat atggcgccca atatgtcggg aaacgccgta
    19741 agcgatcagg cacgcagctt ttatctgtgc gaactggctt tagacttcgc ctcgcaacac
    19801 ctgaaaaccg gcggcagctt tttggtcaaa gtctttcagg gtgcaggcta tcaggaatac
    19861 atggcagcca tgcgcgaaat tttcggcacg gtgcagacgc gcaaacccga agcctcgcgc
    19921 aatcgctcca gtgagattta tttattgggc aaaaataaac gctgacaata cagacggcgt
    19981 gctttacaat catttccgtt ttacacctca attatggagc cttgctaagt ggggaacacc
    20041 tttaaatcaa tccttgtctg ggtcgccttg ggtatcggcc tgatggctgc gttcaacgct
    20101 ttagacggta aaaaagaaga caacgggcaa atcgaatatt ctcagttcat ccaacaggtc
    20161 aacaacggcg aagtatccgg cgtcaacatc gaaggatccg tcgtcagcgg ctacctgatt
    20221 aagggcgagc gcaccgacaa aagcaccttc ttcaccaacg cgcctttgga cgacaacctg
    20281 attaaaacac tgctcgacaa aaacgtccgt gtaaaagtaa cgccggaaga aaaaccgagc
    20341 gcgctggctg ccctgtttta cagcctgctg cccgtcctgc tgctgattgg cgcgtggttc
    20401 tactttatgc gtatgcagac gggcggcggc ggcaaaggcg gcgcattctc attcggcaaa
    20461 agccgcgccc gcctactgga caaagatgcc aacaaagtga cctttgccga tgtcgccggc
    20521 tgcgacgaag ccaaagaaga agtgcaggaa atcgtcgatt acctcaaagc gccgaaccgc
    20581 tatcaaagcc tcggcggtcg cgtgccgcgc ggcatcctgc tggcgggcag cccgggtacg
    20641 ggtaagacgc ttttggcaaa agccattgca ggcgaagccg gcgtgccgtt cttcagcatt
    20701 tccggttccg actttgtcga aatgttcgtc ggtgtcggtg cgagccgcgt ccgcgatatg
    20761 ttcgagcagg cgaagaaaaa cgctccctgc atcatcttta ttgatgaaat cgacgcagtc
    20821 ggccgccaac gcggcgcagg tttgggcggc ggcaatgatg agcgcgagca aacattaaac
    20881 caattgttgg ttgaaatgga cggttttgag agcaatcaga ctgtaattgt gattgcggca
    20941 accaaccgcc ccgacgtact cgatcctgcg ctgcaacgcc ccggccgttt cgaccgccaa
    21001 gtggttgtcc ccctgccgga catccggggg cgcgaacaga ttttgaacgt ccattctaaa
    21061 aaagtgcctt tggacaaatc tgtggattta ttgtccctcg cgcgcggcac gccgggtttt
    21121 tccggcgcgg atttggcgaa cttggtcaac gaagccgccc tgtttgccgg ccgccgcaat
    21181 aaagtcaaag tcgatcagag cgattttgaa gacgccaaag acaaaatcta tatgggtccg
    21241 gaacgccgca gtatggtgat gcacgaagac gaaaaacgtg cgacggcgta tcacgaatcc
    21301 ggacacgcga ttgttgccga aagcctgccc tttaccgacc ccgtccacaa agtaaccatt
    21361 atgccgcgcg gacgtgcgct gggtctgact tggcagcttc cggagcgcga ccgcatcagt
    21421 atgtataaag atcagatgtt gagccagctc tccatcctgt tcggcggacg gattgccgaa
    21481 gacatcttcg tcggacgcat ctccaccggc gcatcaaacg actttgaacg cgcaacccaa
    21541 atggcgcgcg aaatggtaac gcgctacggc atgagcgaca aaatgggcgt gatggtttat
    21601 gcggaaaacg aaggcgaagt cttcttagga cgcagcgtaa cccgttcgca aaacatttcc
    21661 gagaaaaccc agcaggacat cgatgcggaa atccgccgga ttttggacga gcagtatcag
    21721 gttgcctaca aaatcctcga tgaaaaccgc gacaagatgg aaaccatgtg caaagccctg
    21781 atggaatggg aaaccatcga ccgcgatcag gtactggaaa tcatggcggg caaacaaccc
    21841 agtccgccca aggattacag ccacaacctg cgcgagaatg cggacgcggc ggaagataac
    21901 gcgccgcacg ctccgactcg ggaagaaacc gaagcacctg ccccggcaga caccgcttcg
    21961 acagagtccg agcagcagcc tgaaaacaag gcttaacttc ccgaacaaac ggcagcccgc
    22021 aagctgccgt tttcccatcc tgttttcaga cggcattttc cgctcaatgc cgtctgaaac
    22081 gccaaagccg tatcggtttt tgcggatctg cgatatggcg gtttaaattg aaatcagacg
    22141 aggcaaggct gtaccggttt aaaattaaac cgctataata ccgcctttcg aatacacgcc
    22201 cgcattggtg atgcctgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga
    22261 caaaatgttc gagctggtcg ataaggttga agactatccg cactttctgc cgtggtacag
    22321 caagaccgag gtcatcgggc gtagcggcaa cgaactgaag gcgcggctgt ttatggacta
    22381 tatgcacgtt cgccaatcat tcgccacgca caaccgcaac attccgggca gggaaatccg
    22441 tatggaactg ctcgagggtc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt
    22501 gggcgacgat atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt
    22561 gtccgcctta atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt
    22621 caaagaggca gaccgccgtt atgcttgaaa ttgagattgt gtacgggctg cccgaccgac
    22681 aggttttgaa aaccatgcag cttgccgagg gaacaaccgt ccgcgccgcc gcactgcaaa
    22741 gcggtttgga cggcatattt gaagatttaa acctgcattc cgcgcctttg ggcattttcg
    22801 gcaaagccgt caaagacgac acgcccctgc gcgacggcga ccgcatcgaa gtgtaccgcc
    22861 cgctgttgat cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac
    22921 catgccgtct gaagccttca gacagcattc gacagaaaca cggaaaatat catgtcaaac
    22981 aaaatcaaaa tgcttgtcgg attgggcaac cccggcaaag aatacgaaca aacccgacac
    23041 aatgcgggtt tttggttcct cgacgaactg gcgtggaaat ggaaggcttc atttaaagaa
    23101 gaaaaaaaat tcttcggcga agtcgcccgt gccgccctgc ccgacggcga cgtttggctg
    23161 ctcaaacctg ccacgttcat gaaccgttcc ggacaggcag ttgccgcgct tgcacagttc
    23221 tacaaaatca aacccgaaga aatcctcgtc gtccacgacg aactcgacat tccctgcgga
    23281 cggattaaat tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag
    23341 gcaaaactcg gcacggcaga ctattaccgc ctgcgcctcg gtatcgacca cccgggcgac
    23401 cgcaacctcg tcgtcggcta tgtcctgaac aaacccagta cggaacaccg ccgacagatt
    23461 gacgatgccg tcgccaaatc cctgcaagcc atacccgaca tccttgccgg caaatgggaa
    23521 gaagcaaccc gcttcctgca cagcaaatga cccgatgccg tctgaagccc tttcagacgg
    23581 catgttcccg atttccatat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc
    23641 gaatccatcc ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac
    23701 gacgcaccat ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc
    23761 aaattcctgc gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc
    23821 tgccgcgcaa ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc
    23881 tgctcttcaa agctgccgac accgcgccgc cgccgtttcc gcatttcgac aaagcagcac
    23941 accttgccct gtttttcgca caaatctggc ttttgaccaa agcattcaaa accggaaaac
    24001 ttcccatccc ctaccgcagc ctgatggtct ttgccctctg tttcgccctc ttcagcgaat
    24061 gcgcgcaggc atggtttacc gcaacgagaa ccggcagttt gggcgatgtt cttgccgata
    24121 tggcaggtac ggttctcgca ctctttgccg cccgcgccgc cgaccgcccg gactgaatcg
    24181 gtttatttcc cccaaacagg atgcacctct tcccgataca tcctattttt ccccttacat
    24241 atcaatccca tctcataaac aaaaccgaca aatcgtttac aatatattta cactacatca
    24301 gattacaaat atactcgaac cagttgtaga atcggcggtt ccacaaaccg tttcggattg
    24361 cacgacacaa aagaaaaacc gattttattg ccttaaagga gtattcatga acctgcatgc
    24421 aaaggacaaa acccagcatc ccgaaaacgt cgagctgctc agtgcgcaga agccgattac
    24481 cgactttaag ggcctgctga ccaccattat ttccgccgtc gtctgtttcg gcatttacca
    24541 catcctgcct tacagccccg atgccaataa aggtatcgcg ctgctgattt tcgttgccgc
    24601 actttggttt accgaagccg tccacattac cgtaaccgca ctgatggtgc cgattctcgc
    24661 cgtcgtactc ggtttccccg acatggacat caaaaaggcg atggctgatt tttccaaccc
    24721 gattatctac atttttttcg gaggcttcgc gcttgccacc gccctgcata tgcagcggct
    24781 ggaccgtaaa atcgccgtca gcctgttgcg cctgtcgcgc ggcaatatga aagtggcggt
    24841 tttgatgttg ttcctcgtta ccgcctttct gtccatgtgg atcagcaaca ccgccaccgc
    24901 cgcgatgatg ctgcctctag caatgggtat gctgagccac ctcgaccagg aaaaagaaca
    24961 caaaacctac gtcttcctcc tgctcggcat cgcctattgc gccagcatcg gcggcttggg
    25021 cacgctcgtc ggctcgccgc ccaacctgat tgccgccaaa gccctaaatc tggacttcgt
    25081 cggctggatg aagctcggcc tgccgatgat gctgttgatt ctgcccttga tgctgctctc
    25141 cctgtacgtc atcctcaaac ctaatttgaa cgaacgcgtg gaaatcaaag ccgaatccat
    25201 cccttggacg ctgcaccgcg tgatcgcgct gttgattttc cttgccacag ccgccgcgtg
    25261 gatattcagc tccaaaatca aaaccgcctt cggcatttcc aatcccgaca ccgttatcgc
    25321 cctgagtgcc gccgtcgccg tcgtcgtctt cggcgtggcg caatggaagg aagtcgcccg
    25381 caataccgac tggggcgtgt tgatgctctt cggcggcggc atcagcctga gcacgctgtt
    25441 gaaaacatcc ggcgcgtccg aagccttggg acagcaggtt gccgccacct tttccggcgc
    25501 gcccgcattt ttggtgatac tcatcgtcgc cgccttcatt atttttctga ccgagttcac
    25561 cagcaacacc gcctccgccg cattgcttgt accgattttc tccggcatcg ctatgcagat
    25621 ggggctgccc gaacaagtct tggtattcgt catcggcatc ggcgcatctt gtgccttcat
    25681 gctgccggtt gccacaccgc ctaacgcgat tgtgttcggc acgggcttaa tcaagcaacg
    25741 cgaaatgatg aatgtcggca tactgctgaa catcctctgc gtagtattgg ttgctctgtg
    25801 ggcttatgct gtactgatgt aaaccatcga cctaaacaac aagaccgtct gaaagaatat
    25861 ttttcagacg accttgaagt ttgtcgttca gacacaattt gtcgaatcat tcaaaaccgg
    25921 attctaacga aaggaaaccc atgattatcc tgcacaccaa caaaggcgac atcaaaatcg
    25981 aactcgattt cgacaaagcc cccgttaccg ccaaaaactt cgagcaatac gtcaaaaacg
    26041 gcttctacga cggcgtaatc ttccaccgcg tcatcaaggg cttcatgatt caaggcggcg
    26101 gcatggatga aaacatgaac gaaaaagaaa cccgcgatcc gattcaaaac gaagcgtcca
    26161 acggcctgcc caacgataaa tacaccatcg ccatggcgcg tacttccgat ccccattccg
    26221 ccagcgcgca attcttcatc aacactgccg acaacgcttt cctgaacttc cgttctaaag
    26281 agctgtacgg caaaaccgtc gtccaagact ggggctatgc cgtattcggt aaagtcgttg
    26341 acggttttga cgttgtcgat gccatcgaag gcgtttctac caaacgtcat ggttaccacg
    26401 acgacgtacc gagcgaacct gtcgtcatca ttaaagccga agcggtataa accgacaatc
    26461 cgaaagcagc ctgcacaaag gctgctttta tttcagacgg catgttattt gatgtaaccg
    26521 tgctatgccg tctgaaaacc ggacgcaagc cttcagacgg cataccgcta tgttaaaata
    26581 tgcccgtttt tcctaaacgg acacaaagga aaccttatgg caagcatcgc ccgcgacatc
    26641 ttcaaagcct acgacatccg gggtattgtc ggcaaaaccc tgaccgacga agccgcctac
    26701 cttatcggca aagccatcgc cgccaaagcc gccgaaaaag gcattacccg catcgcgctc
    26761 ggacgcgacg gacgcttgag cggtcccgaa ctgatggaac acatccggcg cggctttacc
    26821 gacagcggca tcaatgtcct caatgtcggt atggttgcca ctcctatgct ctacttcgcc
    26881 gccgtcaacg aatgcggcgg cagcggcgtg atgattaccg gcagccacaa tccgcccgat
    26941 tacaacggct ttaaaatgat gctcggcggc gacacgcttg ccggcgaagc catccaagaa
    27001 cttttgtcca tcattgaaaa agacggtttt gctgccgccg gcaaacaagg cagcgtcacc
    27061 gaaaaagaca tctccggcga atacctcaaa cacattaccg gacacatcag gctcaaacgc
    27121 ccgatgaaca tcgccattga cgcgggcaac ggcgtgggcg gcgcgtttgc cggcaaactc
    27181 tacaaaggct tgggcaacga agtaaccgag cttttctgcg acgtggacgg cactttcccc
    27241 aaccaccatc ccgacccatc caaaccgaaa aacctgcaag atttgattgc cgcgctgaaa
    27301 aacggtgatg ccgaaatcgg cttggcgttt gacggcgatg ccgaccgctt gggtgtggtc
    27361 accaaagacg gcaacattat ttatcccgac cgccaactga tgcttttcgc gcaagacgtg
    27421 ttgagccgca atcctaaagc caaagtgatt ttcgacgtca aatccacccg tctgcttgcg
    27481 ccttggatta aagaacacgg cggcgaacct gtgatggaaa aaaccggcca cagctttatc
    27541 aaatccgcca tgaaaaaaac cggcgcgccg gttgccggcg aaatgagcgg acacatcttc
    27601 ttcaaagaac gctggttcgg cttcgacgac ggtctgtacg ccggcgcacg cctcttggaa
    27661 atcctgtctg cctccgataa tccgtccgaa gtgttaaaca acctgccgca aagcatttcc
    27721 acgcccgaac tcaacatcgc cctgcccgaa ggcagcaacg gccatcaggt tatcgacgaa
    27781 ctcgccgcca aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggtctgcgc
    27841 gttgaatttc ccgacggctt cggtctgatg cgtgcttcca ataccacgcc gattctggtg
    27901 ttgcgttttg aggcggatac gcaagaagcc atcgagcgga ttcaaaacca attcaaagcc
    27961 gtcatcgaaa gcaatccgaa tctaatctgg cctctgtaaa cacaggaaaa atgcttaatc
    28021 aaatcttggt agattggatt tggcttaaaa atctccgcgt ccgttcgtgt ttggggtggt
    28081 cgaacaactc tttcgggctg ccctgctcta cgataacgcc gccgtccatc acgacaacgg
    28141 tagtcgcaac ttccagcgcg aacttgattt cgtgggtaac gacgaccatc gtccaacctt
    28201 cccgcgccaa ttccttcatg gcgttcaaca cgtcttgcac caactcgggg tcaagcgcgg
    28261 aagtcggttc gtcaaacagc atcagctcgg gctgaatcgc caatgctcgg gcaatgccga
    28321 cgcgctgctg ctgaccgccg gaaagctggt agggatacaa atccactttg tcgcccaagc
    28381 cgactttttc cagcagtttc agagcctctt cgcgcgcttg ggcggcaggc ttgccctgta
    28441 cggcaaccgg tccttccatc acgttttcca aggcggtttt gtgcggaaag aggttgtatt
    28501 gttgaaacac catgcctgat ttgcggcgca gtgccaaaat atcgtgtttg cttggttttt
    28561 tagaaaaatc gattttcagc ggtcgctcgt tgtcgaactc gatttgtccg tcttcgggca
    28621 tttccaacgc gtttaggcat cgcagaaacg tcgttttgcc tgagccggaa ggcccgagga
    28681 tgacgaccac ctgccctttg cacacatcca aattgatgcc gcgcaaaatg gtattttcgc
    28741 cgaaggtctt atggatattg cggattttaa tcatgacaac tccttatttg gcgacatagc
    28801 ggtcgaagcg tttttccaag cgcgcctgaa tcaggaacag cactttgcaa aaacaccagt
    28861 aaaccaaagc ggcttcgata tagacgggca aaaagtcata agtgcggttt gccgtttcct
    28921 gcgcgacgcg gaataattcc gttaccgtca cgactgccgc gagcgaggtg tttttaaaca
    28981 aaccgataaa ctcgttgctc aaaggcggca cggcaacgcg aaatgcctgc ggcgcgacga
    29041 tgcggcggaa cgtctgcata taggtcatgc cgatggagaa acctgcttcc cattggcctt
    29101 tcggtacgga caaaattgcc gcgcgtatgg tttcggaagc atatgcgccg acattgagcg
    29161 aaaagccgat gatggcggca ggaatcgggt cgatatagat gccgacggaa ggcagcccgt
    29221 aaaacacaat cacaagctga accaacagcg gcgtaccgcg aatgacggaa atataaaatt
    29281 ccaccaattt cagcaggatt ttccgcacga tgccgccggc gggcataatc cgcaccaaag
    29341 ccacggctac cgcaatcatc ataccgataa cgaaagaagc tgccgccaaa ggcagagaga
    29401 ccgcgaagcc ggctttgacc ataggcaaaa acgcgctgac aatcatatcg gcgcgtgttt
    29461 ccgtcataaa cggcagcgaa gcaaggaaat tattgaacac tgatgtcttt tccgaagaat
    29521 tgttcgccca gttttttcag cgtaccgtcg gctttcagct cgttgattgc cgtactgaat
    29581 ttcgccacgg cttcgtcatt gcccttgttg acaatcaggc cggaaccgac tttttcatcg
    29641 gcaggtgcgg accaaacgat tttcacgccc gcattcgggt ttttcttcag atagtccaaa
    29701 accgccaatt cgtcgttcag ggttgcatcg gcacgttttt gttcaatcag ggtcagcgat
    29761 tgcgccaaac cgtccactgc caccaaatct gcgcctgcag ctttggcttt ttcgccgtag
    29821 ttgctggtca gggattgtgc ggttttcacg cctttgatgt cgtcgataga tttgatgttg
    29881 ctgtcgttac gggcaaccaa tacggcaccg ctccagctgt aaggatcgga tttgtcgaat
    29941 gtcgcttggc gttcggggct ggtcagaccg acttggtttg ccaccacgtc gaaacgcccc
    30001 gccttcaaac ccgccatcat cgaatcccat tgcgtttctt taaactcgac tttcacgccc
    30061 agtttttccg ccacggcgcg ggttacttcc acatcgtaac cggtcagttt gccgtccttg
    30121 tcgtggtagg taaacggtgc gtaagtgcct tccgtgccga cggtaaccgt gcctttattg
    30181 ttgatgcgct cgattaaaga accggaaact gccgattgtg caggcgcgga agatgctccg
    30241 ctgccgcttt ccgaaccgcc gcaggccgcc aaaaccaatg cggcaatgcc gccgagtacg
    30301 aattttttca acatcattct ctcctgtaaa aagtgtgcaa gtgcggcatt ctaagtgaaa
    30361 ttgcggcaaa cacaaagaat acttttttta tatggttatt ccaaatcgga atggaaaaga
    30421 tatgccgaag ccgccggaag cggaaataaa gggggcggga aaggcgacgg gcggcacgaa
    30481 accgaaaagc ggcatcaggt atgcaaacgg cggattcacg cacgccgttc cggcaaaaat
    30541 ccgcctgccg attcggtgct ataatccatc cgaaacagat gacaccaaaa aggaacatct
    30601 catgaacacg ccccaatccg ccatcattcc cgaccacgcc caagccggca tctttatcga
    30661 agccgacttc gccgccaacc gcctcaacga tattaaagcc gcctgccgcg cttcgctcga
    30721 cgcgttatcc gccttgaaaa cccgctttcc ggacgatatt ttgggtctga ccatcgcctt
    30781 cggcagcaaa gcctgggcaa cattcggaca tacggacgaa ggcagtgaaa tcaaaccctt
    30841 ctccgaaatg ggcaacgggc ttgcgccgtc cacgcagcac gatatgtcca tccacatcca
    30901 atctttccgc caaaacgccg cctacgcgct tgcccaatcc gttttgggcg cgttcggcga
    30961 cagcatatgc gtcgcgtccg aagaacacgg tttgcgcctg tatcaggatc gcgggctgga
    31021 cggtttcgtc gacggcacgg aaaacccgca gggcgatgaa aatgtccgcg aagtcgccat
    31081 catccccgaa ggaaaacccg atgcgggcgg cagctatgtc ctgctgcaaa aatacctgca
    31141 cgatttgaaa aaatgggatg ccgtccccgt cgccgaacag gaggcctcgg tcggacgcgg
    31201 caaggaaacc gacgacgaat tcggccgcga tgtccgcctg cccgattcgc acctcggccg
    31261 cgtcaacctg aaagaaaacg gagtcggcct gaaaatcgtc cgccgcagcc tgcccttcgg
    31321 caaaatcagc ggcgaacacg gtttgatgtt taccgcatac tgccgcacgc tgcacaatat
    31381 cgaagcgcaa ttattgagta tgttcggcga tacggacggc aaaaccgacc tgctgctcca
    31441 acatctctcc gccgccgttt cgggcggata ctactacgcg ccgtctgtcg aacgcctgca
    31501 aaacctctaa gccggacgat gccgtctgaa accatttttc agacggcatt ttcccgtttt
    31561 atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc
    31621 tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgcactgt ctgcggcttc
    31681 gtcgccttgt cctgattttt gttaatccac tataccgtcc aaccgttttc cgagcgcgca
    31741 ctatgtctgc acccatccaa gcattcgact cgctaaccgt ccccattgcc ggcaccaacc
    31801 tgattgaggc ctctgccggc accggcaaaa cctacggcat tgccgccctg tttacgcgcc
    31861 tgatcgtatt agaacaaaaa aacgtcgagc gcgtattggt cgttaccttc accaaagccg
    31921 ccaccgccga gttgaaaaca cgcctgcgcg cacgtttgga cgatgtgttg caggttttag
    31981 aaagcaaaga aattgccgaa cttggagacg gcgcgctttc agacggcatc gccgcctatt
    32041 gtgcggaaca ccacaaaggc gacaccttcc tgcccgcact cttaaaacag gctttgcaaa
    32101 aagagggccg gacgcgtctg attgtccgcc tcaaggccgc catcgggcaa ttcgacaacg
    32161 ccgccatcta caccatccac ggcttctgcc agcgcatcct gcgcgactac gccttcttgt
    32221 gccaagcacc gttcgatgtc gaactgaccg aagaagacgg cgaccgcctg cttatcccgg
    32281 cgcaagattt ttggcgggaa cgcgtcagca atgatccggt gcttgccgca ttggcgttta
    32341 aacgcaaagc tgtgccgcaa accgtccttg cccaaatccg cgcctacctg tcccgcccgt
    32401 acctgaattt ccgccgtccg caggcggatt tgaaacaggc gcagcgcaac gccgaaacct
    32461 catggcaaac cgtctgccgc ctgctgcccg agctggaagc cggcttttgg cgcatccatc
    32521 ccgacctcaa cggcaacagt taccgcaaaa acagcttcgg caaccttttt aaagaactgg
    32581 cacaaaaatc cgccgccgga caactgccct ttc
//
LOCUS       DAWHXS010000008        32608 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWHXS010000008 DAWHXS010000000
VERSION     DAWHXS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.008"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..180)
                     /locus_tag="WIE22_000300"
     CDS             complement(<1..180)
                     /locus_tag="WIE22_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ4126447.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSM"
     gene            complement(327..2255)
                     /gene="rpoD"
                     /locus_tag="WIE22_000301"
     CDS             complement(327..2255)
                     /gene="rpoD"
                     /locus_tag="WIE22_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ4126448.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(2442..4214)
                     /locus_tag="WIE22_000302"
     CDS             complement(2442..4214)
                     /locus_tag="WIE22_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ4126449.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(4358..7108)
                     /gene="secA"
                     /locus_tag="WIE22_000303"
     CDS             complement(4358..7108)
                     /gene="secA"
                     /locus_tag="WIE22_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ4126450.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHTLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            7239..7661
                     /locus_tag="WIE22_000304"
     CDS             7239..7661
                     /locus_tag="WIE22_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ4126451.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            7942..8493
                     /gene="azu"
                     /locus_tag="WIE22_000305"
     CDS             7942..8493
                     /gene="azu"
                     /locus_tag="WIE22_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ4126452.1"
                     /translation="MKTYLALISAAVIGLAACSQEPAAPAAEATHAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            8588..9097
                     /locus_tag="WIE22_000306"
     CDS             8588..9097
                     /locus_tag="WIE22_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ4126453.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            9131..9751
                     /locus_tag="WIE22_000307"
     CDS             9131..9751
                     /locus_tag="WIE22_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ4126454.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            9865..11010
                     /gene="dapE"
                     /locus_tag="WIE22_000308"
     CDS             9865..11010
                     /gene="dapE"
                     /locus_tag="WIE22_000308"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ4126455.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            11382..11661
                     /locus_tag="WIE22_000309"
                     /pseudo
     CDS             11382..11661
                     /locus_tag="WIE22_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(11750..12274)
                     /locus_tag="WIE22_000310"
     CDS             complement(11750..12274)
                     /locus_tag="WIE22_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ4126456.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGDNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVRRRN
                     AASLQLSRCASIADDPSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     ALCGTSSSVSTAAA"
     gene            12585..13337
                     /locus_tag="WIE22_000311"
     CDS             12585..13337
                     /locus_tag="WIE22_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ4126457.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGGKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDALLAIPYGETRSYKEQAQHLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            13528..13603
                     /locus_tag="WIE22_000312"
     tRNA            13528..13603
                     /locus_tag="WIE22_000312"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13561..13563,aa:Asn,seq:gtt)
     gene            13734..14744
                     /gene="waaF"
                     /locus_tag="WIE22_000313"
     CDS             13734..14744
                     /gene="waaF"
                     /locus_tag="WIE22_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ4126458.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            14799..15245
                     /gene="smpB"
                     /locus_tag="WIE22_000314"
     CDS             14799..15245
                     /gene="smpB"
                     /locus_tag="WIE22_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ4126459.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            15612..17816
                     /locus_tag="WIE22_000315"
     CDS             15612..17816
                     /locus_tag="WIE22_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ4126460.1"
                     /translation="MYYETRSKQASKQKFKKSFIISLFFSILYTSPLLAVDYVYDKTK
                     LTDDEITRLKKLRDRNSEYWKDDLFRIDIPKETGLRHDIEGAAKGNFSYPIFYIPTDD
                     KPIDPDKKISFFDSPYTPGYTAAFVQGFGVKERNGYTEEQAKKYIDELRTKLKTAPII
                     AAAQLGYFQLNDGSGDSWLYNYYRNDIFNSYGEIKHSNINSEILAVGNVADLYYKHFI
                     RNPFIYVDWSIFSLPLPRLKVSENSHVIGQIIHLYRLDLEDSLWEPRWDSDVSYLNLF
                     NSHIRFNTKNDSLVVGESRIRPTPDNALNTEKYLKSQFSDTGYHSSVRSTYIGYPSIR
                     FAYDLSEREADKPVLTLKSKVTGKTAIVFEEKALNNLKNLTYRQLIKTETDVEPDAFF
                     LLEEYKKGRYRLFLRQCPNGFCIGVEKLAIPTHLVASYAQQAQAANTLFNLRLNDKNS
                     DIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSVG
                     LMSGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQRFR
                     HRINTEDATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGK
                     FSDSENAHVNLLGSRQLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEIDG
                     ERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(17997..19364)
                     /locus_tag="WIE22_000316"
     CDS             complement(17997..19364)
                     /locus_tag="WIE22_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ4126461.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENSVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(19474..19800)
                     /locus_tag="WIE22_000317"
     CDS             complement(19474..19800)
                     /locus_tag="WIE22_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126462.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPAAEAAATEAPAAEAPAAEAPAAEAPAAEAPTAEAAATEAPAAEAPAAEAPAAEAP
                     AAEAAK"
     gene            20273..20755
                     /locus_tag="WIE22_000318"
     CDS             20273..20755
                     /locus_tag="WIE22_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ4126463.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            20842..21654
                     /gene="hpnC"
                     /locus_tag="WIE22_000319"
     CDS             20842..21654
                     /gene="hpnC"
                     /locus_tag="WIE22_000319"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ4126464.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLDALRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(21725..22195)
                     /locus_tag="WIE22_000320"
     CDS             complement(21725..22195)
                     /locus_tag="WIE22_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ4126465.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSVLEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            22286..24127
                     /locus_tag="WIE22_000321"
     CDS             22286..24127
                     /locus_tag="WIE22_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ4126466.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            complement(24643..25839)
                     /locus_tag="WIE22_000322"
     CDS             complement(24643..25839)
                     /locus_tag="WIE22_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ4126467.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            26190..26489
                     /locus_tag="WIE22_000323"
     CDS             26190..26489
                     /locus_tag="WIE22_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126468.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            26489..26674
                     /gene="ccoS"
                     /locus_tag="WIE22_000324"
     CDS             26489..26674
                     /gene="ccoS"
                     /locus_tag="WIE22_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ4126469.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            26664..27974
                     /locus_tag="WIE22_000325"
     CDS             26664..27974
                     /locus_tag="WIE22_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4126470.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            28132..28866
                     /gene="dnaQ"
                     /locus_tag="WIE22_000326"
     CDS             28132..28866
                     /gene="dnaQ"
                     /locus_tag="WIE22_000326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ4126471.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGKVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGADA"
     gene            28863..29552
                     /locus_tag="WIE22_000327"
     CDS             28863..29552
                     /locus_tag="WIE22_000327"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ4126472.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            29584..30066
                     /locus_tag="WIE22_000328"
     CDS             29584..30066
                     /locus_tag="WIE22_000328"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ4126473.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTASEFKDADSRALLRDAYRSVQAKGWHVVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            30143..30814
                     /gene="rpiA"
                     /locus_tag="WIE22_000329"
     CDS             30143..30814
                     /gene="rpiA"
                     /locus_tag="WIE22_000329"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ4126474.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            30883..31554
                     /locus_tag="WIE22_000330"
     CDS             30883..31554
                     /locus_tag="WIE22_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ4126475.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWTDSVGE
                     WLGIW"
     gene            31733..32479
                     /locus_tag="WIE22_000331"
     CDS             31733..32479
                     /locus_tag="WIE22_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ4126476.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSGGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         7412 a         7647 c         9264 g         8285 t
ORIGIN      
        1 catggacagc aggggatagg caggacggcc gcggtggtct cgaaggtaac gggttttttg
       61 acgattcagg tattgttcga tcggctgcca atcaatcacc tggtccaact tcaatagcgg
      121 gaaacggtcg atgtgtttgg caatcatggc ttgggcggtt tgttggaaga aggtgctcat
      181 ggaaaatccc ctaaatggct tggtgggaat ttaggggatt ttggggaatt ttgcaaaggt
      241 ctcgggatag gagtgtggga tatgtggaag cgttgtttat tgtgtaagaa aaaaccgcag
      301 gtatttgaaa cctgcggttt tttggtttac agcttgctgt cttcgctgtc caagaaactt
      361 ctcaaacggt cgcttcttgt cggatgccgc agcttgcgga gtgcttttgc ctcgatttgt
      421 cggatgcgtt cgcgcgttac gtcaaactgt ctgccgactt cttccagcgt gtggtcggtg
      481 ttcatatcga tgccgaaacg catacgcagg acttttgcct cacgcggggt caggctttcg
      541 aggatttctt tggttacttc gtgcaggctg gtgtacattg ccgcatcggc cggcgcaaca
      601 ttgttggcat cttcgatgaa gtcgcccaag tgcgaatcgt cgtcgtcgcc gatgggggtt
      661 tccatcgaaa tcggctcttt ggcgattttc atgattttgc ggattttgtc ttcgggcatc
      721 tgcatcagtt cggcaagttt ggcggaatcg ggttcttcgc cggtttcttg aaggtgttgg
      781 cgcgagatgc ggttcatctt gttgatggtt tcaatcatat gtaccggaat gcggatggta
      841 cgcgcctgat cggcaatcga gcgtgtaatt gcctggcgga tccaccaggt tgcgtaggtg
      901 gagaatttat agcctctgcg gtattcgaac ttatcgaccg ctttcatcaa accgatgttg
      961 ccttcctgaa tcagatcaag gaattgtaag ccccggttgg tgtatttttt ggcgatggaa
     1021 atcacgaggc gcaagtttgc ctgaatcatt tcctgtttgg cggctgcggt ttctttttcg
     1081 ctcgacacca tatttttgtt gatttctttc aactcttcga tggaaatgcg ggtttctttt
     1141 tccatatccg ccaactcggt ttgtttttcg aggatggcgt ggcggaagcg gtcgagcgcg
     1201 tcgctccaaa ccctgccttt ggcgatttct tcttcaatcc attccagatt ggtgatttcg
     1261 ggcaggaagt tttggatgaa gtagtcgcgc tccatatgga cgcggtcgag gcagatgtcg
     1321 cggatttcgc gttcgagttt gcggatgttt tctacttttc cgcgcaggct gctgctgagg
     1381 ctgtcgattt gccgggtggc gaaacggact tccagcagtt tgttggcaat cgcgtcgcgg
     1441 taggcgagat agtctttgtg ccggctgtgg tgtttttcca aacggccaat catttttttg
     1501 tagtcttttt cgatttgggc aaagtggccg atgacttttt gtttcaattc ggcgagattg
     1561 gctgccgaga tttcatccgc atcttcttcc gattcttcgt cgtcttcgtt ttcgtccgaa
     1621 ttgtcgttgg aaggtttctc gggtgctgtg gtttccaagt gccccaagcc caattcgttg
     1681 agcaatactt cattcgggtc gataatggct tctacgactt cgtcgacgcg gatttcgtct
     1741 ttgcggattt tttcgatgag ttctaagatt tccgcaatgg aacccgggca ggcggagatg
     1801 gcctgaacca tatttttcag ggcgttttcg atttttttgg cgatgatgat ttcgtcttcg
     1861 cgggtcagca ggtcgacctg ccccatttcg cgcatataca tacggacggg gtcggtcgtc
     1921 ctgccgaact cggaatctgc gctggaaagg gcggcctcgg cttcttcgac ggcatcatcg
     1981 tcggtaacgg cggcattgtc gcttaacaat atgtcttccg catcgggggc gtgttcggta
     2041 acttggatgc ccaaaccgga aatcatactg acgatgttgt cgatttgatc ggcatcggac
     2101 atatcgtcgg gcagtgcgtc attgatttcg gagtaggtga tgtagccgcg ttctttgccc
     2161 ataatgatga gttggcgcag gcgcgcgcgt tgttcctcaa tcgtcagggg gcgggcatcg
     2221 tcttggtatt cttcgtgatt ttggtttttg gacattgtct gctttctgtt ggcaagatgc
     2281 cgacggtttg cgtttggacg cacctgaagg cgtggcagag tagggtactc tgatgccgtc
     2341 tgaaggcttt tggggatggg tgttctgcag gtgtcgttgt tgcagccggg gtgaatgctt
     2401 ttcagacggc ataacggttt acggatggcg gaatccgccg gtcaattttg ttttgcggtc
     2461 agcagcgaca gcaaaagttt tttctcgctt tcatttaagc cggattgcag gcttttttgt
     2521 tttaatgttt cgatttggct gtattttaac tcattgagca gttttttgat gccgatttgg
     2581 aagttttcgc aatcttcttc gccgccgcct tccatttctt ccgattgaag ggccgaccgg
     2641 aagatgcggt tgattgtttc ttcgtagggc gaaccgcgca tatgttccaa aacctgtgcg
     2701 gttgccggca cggaaggatg gtttttaatg gtttcggcga ggttggcaag gcaggcgaaa
     2761 tcgccgtcca acgccagata atcgggcagg tctatatatg cagcccaatc cggatttatc
     2821 aagaggctgc ggatttgccg ttgcaccaat gtcggcataa cgggctgttt gacggaaatc
     2881 ggcggcagtt tgtagttttt ttgtttgacg tgccgcttcg gcgcttcctg tcccagcagt
     2941 tgcgcgaggt tgtcggggtc gatgccgacc agttcgctaa gccgttgttt taacaaataa
     3001 gccaatgccg gcgcggtaat ctgcgccaaa agcggcgaac tggtttttac caattccgcc
     3061 ttgccttcct gcgtattgag atgaatgccg tctgaaaggt gttcccagaa atactccgac
     3121 aagggcttgc tttgattcag aagcgcgtct tcaaattgcg ctttgccgta ggcacggatg
     3181 taactgtcgg ggtcgtgttc ttccggcagg aacaaaaaat gcagcgattt gtcgtccttc
     3241 aactgcggca gcgcgttttc cagcgcgcgc caagccgctt tgcgccccgc gctgtcgccg
     3301 tcgaaacaga aataaatact gtccgcctga cgcattagga ttttgacgtg ttcagctgtt
     3361 gttgccgtgc ccaaggccgc cacaccgtag cccacgccga actgtgccag cgcgaccacg
     3421 tccatatagc cttcgaccac cagaatccgc tccgcttcct tgacagcggc acgcccctca
     3481 tacaagccgt aaaggttttt ccccttatca aacaaaggcg tatcgggaga gtttagatat
     3541 ttgggtttgg agtcgtccag taccctgccg ccgaaaccga tgacctgccc gcgcggattg
     3601 cggatgggga acataatccg atgacggaat cggtcgtaat accgcccctc attgtcaatc
     3661 accatacccg tatccaccaa cgcggtattc gggtacggtt ggaatacttg cgccaaaggc
     3721 tgccagccgt cgggcgcgta gcccaaacca taatgcgcga tgacttccgc gctcaagccg
     3781 cgcttgtcca aataagcctt cgccgtcgga ttgaatttca actgttgcgc ataaaaatca
     3841 gccgccgccg ctgtcgtttc ttccagcgtc tgctgctttt tcttacgttc ggcacggact
     3901 tcgggattat cgttctgccc gcgcacttta ggcacaatca tacccacgcg gtcggcaagg
     3961 aactgcactg cctccggaaa cgacagcccc tgatgttcca tcacaaaacc aatcgccgaa
     4021 ccgtgcgccc cgcaactgaa acagtggtaa aactgcttgg tcggactgac cgaaaacgac
     4081 ggcgtttttt ccttgtggaa cgggcaacac gccatatagt tcgccccgcc ttttttcagc
     4141 ggaacctgtt cgtcgatgat gtcgacaata tcggttttgg ccagaagttc gtcaatgaag
     4201 tcggatggaa tcatagttcg gcggggcgga tgcctgtttt tagtttggac ggtacgaaat
     4261 cgggattttt cggaatggcg gtcggatgtg gcggttcagg caaaatgccg tctgaagccg
     4321 gaaaccggat gttcagacgg catttgcgtt caaacgctca agccagtttg ccgtggcatt
     4381 gtttgtattt caaaccgctg ccgcaggggc aggggtcgtt gcggtggacg atttgtcccc
     4441 gcgcttccaa ggcttcgggg ctgaaatccg tcccatcggg attaaaggct tcggtaacca
     4501 gatcggtttg cgactgaccc aaaagttctt ccatatcggg cgattcggaa tggatggact
     4561 ggatgttgcc gatgggttgc tcttcaacca ccgcgacagg gttttgttcg atttgaaccg
     4621 aggtaagcag ggaggcaata tggaatttga tgccgttcca caggtcttgg aacatggtaa
     4681 aggcttcacg tttatattcc tgcttcggat ttttttgggc atagctgcgc aggtgtatgc
     4741 cttgtcgcag gtagtccata gcggcgaggt gttcgcgcca ttggttgtcg atgacctgca
     4801 acatcacgtt gcgctcgaaa tcggccattg cctgcttgcc gaccagttcg gttttggcgg
     4861 catattcgtt ttcgatgcgt tcgatcaggc gttctttgat gtcttgaccg tcaatcgcat
     4921 tgtccgcctt cagccaggat tggatgtctt cgtgcagtct gaattcggca gccagacggt
     4981 tctccaaagt cgggatgtcc cattgttctt ccatgctgtc gggcggcata taggtatcca
     5041 cgaggtcgct gacgacatca gaacggattt cctgcatcag gtcgctgatg tctttgctgg
     5101 tcagaatttc gttgcgctgg ctgtaaatga ctttgcgctg ttcgttggca acgtcgtcgt
     5161 attccaaaac ctgtttgcgc atatcgaagt ttctgccttc gactttgcgt tgcgcccctt
     5221 cgatttggcg cgtcagcagg ttgtgttcga tggcaacgcc gcgttcgggg gcgagacggt
     5281 tgaggatggc ggcggcgcgg tcgagtgcga ataagcgcag cagcgggtct tcaaacgaaa
     5341 ggtagaaacg gctggatccg gggtcgccct gacggccgga acgtccgcgc aattggttgt
     5401 cgatacggcg gctttcgtgg cgttccgtac cgatgatgtg caggccgcct gcttccatta
     5461 ctttatcgtg ttctgcctgc cagccgtttt cgagtgcggc gatttgtgcc tgtttctctt
     5521 cgtcgctcaa ggtttcgtcg gcacggatgg catcggtttg gtgcttcagg ttgccgccta
     5581 aaacgatgtc cgtaccgcgt cccgccatat tggtggcaac ggtaatcgcg ccgactttgc
     5641 cagcttgggc gacaatcagg gcttcgcgtt cgtgttcttt ggcgttgagg acgttgtgcg
     5701 gtagaccggc tttttgcaaa agatgggaga ccagttcgga gttttcgatg ctggttgtgc
     5761 cgacgaggac gggctgccca cgtttgtggc attcttcgat gtctttgacg acggcttcga
     5821 atttttcttc ggcggaacgg aaaatctggt cgttgaagtc tttgcgttgt atggggcggt
     5881 tggtcgggat gatgacggtt tcgaggttgt agatgctttg gaactcgaag gcttcggtat
     5941 cggctgtgcc ggtcatgccg gagagcttgg tgtacaggcg gaaatagttt tggaaggtga
     6001 tggaggcgag ggtttggttt tcgcgtttga tttccacgcc ttctttggct tccacggctt
     6061 gatgcaggcc ttcggaccag cggcggccgg acatcagacg gcctgtaaat tcgtcaacga
     6121 tgacgatttc gccgtcttgg ataacgtaat gttggtcttt gtggaataac gtatgcgcgc
     6181 gcaatgccgc cataaggtgg tgcatcaggg cgatgttggc ggcagaatag agcgagtcgt
     6241 tttctgccag caatcccatt tgggtcagaa tctgctcggc gtgttcgtgg ccggcttcgc
     6301 tcaggatgac ctggtgcgcc ttttcatcta cccagtagtc gccttcgcct tcttctgtct
     6361 cttggcggac gaggtggggc gggacggtgt tcatgatttg gtacaactgg atgttgtcgt
     6421 ctgcctggcc ggagataatc agcggagtgc gcgcttcgtc aatcaagatg gaatccactt
     6481 catcgacaac ggcaaaattc aattcgcgct gcactttgtc gtattggtcg gtaaccatat
     6541 tgtcgcgcag gtagtcgaag ccgaattcat tattggtgcc gtaggtgata tcggcggcat
     6601 aggcgttttg acggtcgaac ggctgcatat ctgaaataat cacgcccacg gtaaggccga
     6661 ggaaattata gagcggttcc atgatgcccg catcgcgtga ggcgaggtag tcgttgacgg
     6721 taacgacgtg tacgcctttg ccggccagcg cgttgagata gacggcgagg gtggcgacca
     6781 gggttttgcc ttcgccggta cgcatttcgg cgattttgcc gtcatgcagc accataccgc
     6841 cgataagctg cacgtcgaag tggcgcatac cgagggtgcg gcgggacgct tcgcggcaga
     6901 cggcgaaggc ttcgggcaaa atgccgtcca aagtctgacc gtcggcgagg cgttgtttga
     6961 attcggcagt tttggcttgc agatcagcat cgcttagggc ttgcatctgt tcttcgagcg
     7021 cgttgattct ggcaacggat ttacggtatt gtttcagcaa gcggtcgttg cggctgccga
     7081 agattttctt ggcaatgttt gtcagcatga ataattcctg cgcctgatat aatagtgggc
     7141 gaaaacgcga ttttaacaca agcgtttgtg caaagtgcta atgccgtctg aaataaaggc
     7201 gcgtttgcgg tatttcaatc tttttgggtg tatgttttat gaatttggaa cagttgggca
     7261 ggcgggacgc gctgctttcc ggacttttga aacaggcggg acagtggcgg cggttggatg
     7321 ccgccgtgaa aaaactgctg cctgccaacc tgcacccgca ttttcagacg gcatgtatcg
     7381 aagacggcag gcttgtcctt ttggcggcaa acaatatggc ggcatcgcgc ttgaaaatga
     7441 ttgcaccgtc ggtattgccg cagttggcag ggcttgatgc ttcgatacgc tctgtttcgg
     7501 tcaggctggc cccgaaaccg gaaaaaccgc cgaaaaccaa taccctgcat ttgagtaagg
     7561 ctgcgctgga gagtttcggt tcggcggcgg taaagttgga aaaacggcat cccgaattgg
     7621 cggaggcatt ggcaaacttg gttagaaggc atggcgcata aaatgtatac gggaatttgt
     7681 gtaaacatcc gttaatatta agaagtaaag gataatgggt ctaatactaa agaaataggt
     7741 tcggggtaaa attgcccctt ttaaagtaaa cgattgtaaa cttgcagaca ggctttgatt
     7801 tcaaatgaaa tttgtagcaa aatgccgccc cgaaacatct gtttgtgcaa cgcggcggaa
     7861 tctttttcaa ggttttgtta atggcggttg cactttgatt tccgtaaaac cgaatattat
     7921 tttatcgatt ggagatttgt tatgaaaacg tatctggctc tgatttctgc tgccgttatc
     7981 ggtttggctg cctgctctca agaacctgcc gcgcctgctg ccgaggcaac tcatgctgct
     8041 gaagcacccg cttccgaagc acctgccgcc gaagctgctc ctgcagatgc tgccgaagcc
     8101 cctgctgccg gcaactgtgc ggcaactgtc gaatccaacg acaatatgca gttcaacacc
     8161 aaagacatcc aagtcagcaa agcatgtaaa gagttcacca tcactctgaa acataccggt
     8221 acgcaaccca aagccagcat gggtcacaac cttgtgattg ccaaagctga agacatggac
     8281 ggcgtattta aagacggcgt aggtgctgcc gataccgact atgtcaaacc tgacgatgcg
     8341 cgcgttgttg cccacaccaa actgatcggc ggcggcgaag agtcttccct gactttggat
     8401 cctgccaaac tggctgacgg cgaatacaaa ttcgcttgta ccttcccagg tcacggtgct
     8461 ttgatgaacg gcaaagtgac tttggtcgat taatccgctt aaagtctcaa aagacggaca
     8521 gcctgctttg tgcaggctgt tttattataa aatgactgct tgaaaagtgc ccgttgagaa
     8581 cgaaaacatg aatccgtttg aaaccaaaag cgttaccttt gccgaaccga ttgaaatgct
     8641 gtatgcctgc cacggcaaag tgcgccgttt ttgcggacaa gtcgccatgc tgtcggacta
     8701 tatcgccgaa aacggctgca atcagattgt tttgcaaacc atccgccaaa tcgcccagta
     8761 tttcaacgtt gccgcgccgc tgcaccatga agacgaagaa gaaaacttct tcccgctgct
     8821 gctgcaatat gcgccgcaag cccaagaaag cgtggacgag cttttgcgcc aacatatcgg
     8881 gctgcacgac aactgggcgg ctgtttccgc cgaatttgcc aaactcgaag cagacaacgc
     8941 ttatgtcccc gatgaggaag cgttcaaacg ttttgtggcg ggatatgatg ttcatttggc
     9001 gattgaagag ccgctgtttg atatgggcaa cacctttatc ccaaaagaaa aactgaccga
     9061 aatcggcgaa attatggcgg cgcgccggcg caaataatgc cgtctgaaac aatcgtctca
     9121 aggatatatt atgctgaccc cgaaaagttg cgatttgttc aatatcccgt ttttccagtt
     9181 tgcccagctt aaaaaatacc agcctgaaag cattccgcaa atcaaagccg actacaagga
     9241 aaactggcag atatggcagc agctgattca gcaggttgcc gccgaattgg gtgcgccgtt
     9301 tgcgccgccg cacatcgaac gctggtgcaa cggctggcag gttcgcgccc atttctttgc
     9361 ctacttcaaa tacgaacaat ataaaaattc cgccgcgatt ttgtcgatac tgctcaaccg
     9421 ccgccgtttg agtgtcagct tggactggca ttgctacaaa gccgacgtgt cgccgattgc
     9481 gctgcctgat tacaaccgtt ggttggataa ttttgatact gaaaaatacg cttctttcga
     9541 tatgtggcac ggcgcggaaa gcgaatacga cgactaccgc actgtcgcgc agcaaaatga
     9601 atccgaccga aggttgcaaa acgacgaaga tttcttctgc atcggcaaac acatcgaacg
     9661 cgacgatttg ggcaggcagg atgtcgcgaa atggatagcg gaaacggtgg aagatttgct
     9721 gccgctttac gaagcgtgtc acggcaaatg attcagaaga ctgacgggta aaaataccca
     9781 ccccgtatgc caacctaacc taaatgccgt ctgaaaatca cattcagaac gtttcagatg
     9841 gtctaatttc aaaagtgaac aaatatgacc gaaacccaat ccctagaact ggccaaagaa
     9901 ttgatttccc gcccatccgt tacccccgac gaccaaaatt gccaaaaact ccttgccgaa
     9961 cgcctgcaca aaatcggttt tgcggctgaa gaactccatt tcggcgacac caaaaacatc
    10021 tggttgcgac gcggcacgaa agctcccgtc gtctgttttg cagggcatac cgacgttgta
    10081 ccgacaggcc ctgtcgaaaa atgggattcg cccccgttcg agccggccga gcgcgacgga
    10141 agattatacg ggcgcggtgc ggcggacatg aaaaccagca tcgcctgttt cgttaccgcc
    10201 tgcgaacgct ttgttgccga acaccccgat catcaaggca gcatcgcgct cctgattact
    10261 tccgacgaag agggcgacgc gctggacggc acgaccaaag tcgtcgatgt gttgaaagcg
    10321 cgcggcgagt tgattgacta ctgcatcgtc ggcgaaccga cagccgtgga caaattgggc
    10381 gatatgatta aaaacggccg gcgcggctcg ctgtcgggca acctgaccgt caaaggcaag
    10441 caaggccata ttgcctatcc gcatttggca atcaatcccg tgcatacttt tgccccggcc
    10501 ttgttagagc tgacgcagga agtctgggac gaaggcaacg aatatttccc gccaaccagc
    10561 tttcaaattt ccaatatcaa cggcggcaca ggcgcgacca acgtcattcc gggcgagttg
    10621 aacgtcaaat tcaatttccg cttctccacc gagtccaccg aagcagggct gaaacaacgc
    10681 gtccacgcca ttttggacaa acacggcgtg caatacgatt tgcagtggtc gtgttcgggg
    10741 cagccctttc tgacccaagc gggcaaactg accgacgtgg cacgcgcagc cattgccgaa
    10801 acctgcggca ttgaggccga attgtccacc accggcggca cttcggacgg acgcttcatc
    10861 aaagccattg caaaagaact catcgaatta ggcccgtcca atgccaccat ccaccaaatc
    10921 aacgaaaacg tgcggctgga cgatattccg aagctgtctg cggtgtatga ggggatattg
    10981 gcgcgcttgt tggctggaaa tgccgtctga aagcagcttg cttgccggat acggatgtcc
    11041 gaccatttgc ttagacctaa cgtgggtttg agataacaca ttgatttttt atgtagagcg
    11101 atttatttca acggcaatcc attccctctc ccgtgggaga gggttaggga gagggcatcg
    11161 gggcggtaac ggaaatattg tctgtatctg ccccgtttta ccctctcccc aaccctcccc
    11221 cacaggggag ggagtcaagt tgcaggcaca ccgacggaat atggttgaaa ctcggccatt
    11281 cggggcagtg ggattttggt ttatttttgg gaagtaaccc cttccaaatc agggtaaaca
    11341 gggctgcgcc ttatgtgcgg ccctgcgtgt tggagataat agtgccgtca aattactttc
    11401 atacgcgcga acgattgggt gctttgggcg gcatcgtgca acatcatacc gccttgtacc
    11461 gcagcgtggc ggtatatgaa ccagctgtgc ttatcgtgcg gcgcggaagc aagaagctgc
    11521 ggtgggcgag gcgcgaattg aggattgcgg cgggtgaggc ggttgcgttg gcaggcggac
    11581 agacgtttga cgttattaat attcccgatt cagacggcct ctatcaggcg caatggattg
    11641 ctttcgaaca ggaaaccgta gaaaggtttg ccgcacaata cgggacggcg caggcagtgt
    11701 gcgatgcggt gaagctgccg caccctgggc ggatgggcgc ggcgtttgat tatgcggcgg
    11761 cggtgctgac ggatgaagaa gtgccgcaca atgcggcgga ggccgcgctt tgcggcgtgt
    11821 tggcgtggtt gcagcatgac ggcatcggtt ttgccgtgta cgaaggcgtc aacctgatgc
    11881 ggcaaatccg caaactgatt accgccgata tggctgcgga cgggtcgtcg gcgatgctgg
    11941 cgcagcggct taattgcagc gaggccgcat tgcggcggcg gacatcggcg ggaatgtcgg
    12001 cgaccacccg gtgcatccag cccagtccgg tcggcgcgtc tttatcgtaa acggtcagga
    12061 cgaaactttt tgtcccggcg ggcggatttt tccacgacag cgcgggcgga gcattgtcgc
    12121 ctgaacagcc gaagccgtaa ggcgcactca aaagctggtt ttgcgtgaag ccgccgtctt
    12181 cggacgggtt gtcgaattgc agcgtgaatg cgcccccggc aaaggcagcg gttgaaaaag
    12241 ccagcagtac ggcggagagg atgcgggttt gcatagatga ttcctttgct tgatgaataa
    12301 gtggattcat tataggctgt caacggcggc aggaatgtgc cgaaacggtt ggatgatgtg
    12361 ccggaatgcg taaatgcaaa aatcgtggtt tcagacgacc ctttgcaggt cgtctgaagt
    12421 ctttaattcc tttccctgag gctttagaaa tatcgcatat ttaggcattg ccgcaaccca
    12481 accctcaaga tttacgtcat agcccgttcc tgcatcggcg tgcgggaatg acgagaattg
    12541 gggattttgc cgccaaatcc atgatagaga tacggagcaa acgaatgatt accctgcctt
    12601 ccttaaacaa tctgccgtca aaatgggatg aaatccgcca ttgcctcgaa acccgggttt
    12661 ttgagtgcgg cgttatgccg catcccaatc tgacggaatg cgaagccggg cagttcgatc
    12721 gggatttttg ggacaatatc ggctgcgcgc ctgaagaata tgtccgtatc cgccgagcca
    12781 tccgtctgtt ggaagtgcgt tatcccgaca gcttgaacga gctggtgtgt gccgccattg
    12841 ccacgccttt gggcgagatg ctggcggtgt tcggcggtaa aggcttgtgt ttgctggaat
    12901 ttgtcgggca gaagcatttg gaacaggaaa ttgccgccgt ccaaaaagcc ttgcgcggac
    12961 ggtttgtgtt tcgggaggat gagcggacgc aacttttgcg gcaggaattg gatttgtatt
    13021 tccaatgccg tctgaaaacc tttgccacgc ctttggaaat gattggtacg gcgtttcaaa
    13081 aacaggcgtg ggacgcgctt ttggcgattc cttacggtga aacgcgcagc tacaaggaac
    13141 aggcgcagca tttgggcaac cccaaagccg tccgcgccgt tgccgccgcc aacgggcaga
    13201 acaaggtgtc tgtcctgatt ccctgccacc gcgtcattgg cagcgacggc aaactgaccg
    13261 gctacgcagg cggtttgaac cgcaaacagt ttttactggc tttggaacgc ggcgaagttc
    13321 agacggcatt gttttaagcg ggggagggca gaacggctca tcgggcttaa atatggtgtg
    13381 atgacggctg tggaaattca tacaacggca atccttttaa ggcatcggtc ttaatgtctt
    13441 tctgattcat atgcttgtcg ttttgtcgcc catttttcca aaattaagga ttgacacatt
    13501 ttttccggac gctataatac gccccattcc ccgatagctc agtcggtaga gcgacggact
    13561 gttaatccgc aggtccctgg ttcgagccca ggtcggggag ccagaatttt gaagcccgaa
    13621 cttgaatgtt cgggcttttt catatttgca cgtacggcgt tcggaatgag acaagatagg
    13681 ttttccaacg gtttcgcggt aaaattctgc ctgtctgatt tcggtttctt gttatgtcca
    13741 tcaaaatcct gattatttct cccagttgga taggcgactg cgtgatgacc cagcccttgt
    13801 tccgccgttt gaaggaactt caccccggtt gcacgattga tgtgttcgcg ccgaagtggt
    13861 cgatggcggt gttcgagcgt atgccggaag tgaatgaaat tcttgagaat ccgttcggac
    13921 acggtgcgtt ggagttgaaa cgccgctggc gggtcggcag ggatttgggg cggcgcggat
    13981 acgatcaggt tatcgtgttg cccggttctt tgaaatcggc agtcatcgcg ctagcaacgg
    14041 gtattgccaa aaggacgggt tatgtcggtg aaagccgtta ttttctgttg aacgatatac
    14101 gcaggctgga taaggaacgt ctgcctttga tggtggacag atatacggct ctcgcgcatc
    14161 cgagtcagga agattttgac gggcattcgg gattccccga gttttccatt gatgaacggc
    14221 ggcgggaaat ttctgtcgaa actttcggtt tgaatcttgg aaagcctgtt ttggcatttt
    14281 gcccgggcgc ggaattcggg ccggcaaagc gttggccgac aaggcatttt gccgagttgg
    14341 gcaaacatta tctggcggcg ggttggcagg tttggctgtt cggttcgcaa aaagatgatg
    14401 aaattgccga ggaaatcaac tgcctttcag acggtatgtg tgtcaatttg tgcggcaaaa
    14461 ccgatttgtc gcaggcaatg gatttgctgt cgttggcgga cacggttgtg tgcaacgaca
    14521 gcgggctgat gcacttggcg gcggcattgg gcaggaaggt ggtggcggtt tacggttctt
    14581 ccagcccgac gcatacgccg cctttgagcg acagggcgaa aatcgtcagc ctgcacttgg
    14641 aatgttcgcc ctgttttaag cgggaatgtc cgctggggca taccgactgc ctcaacaggc
    14701 tgtatcccga gaagattgtg caggcggttg aagaggcggt atgatgggtt ctgttttcgg
    14761 gtttatgccg tctgacggcg gatattgata ggatgtttat ggctattgcg aacaataaaa
    14821 aagcatttca cgattttttc attgaagacc ggattgaagc cggtttggtc ttggaaggct
    14881 gggaagtcaa agcaatccgc gccgcgcgcg tacagcttaa agagagttat atctattgga
    14941 aaaaagacgc gttttatctg gtcggctgcc atattacggc tttgcccaca gcctcgacac
    15001 acattaaacc agatgccgta cgtccgcgca agctcttgtt gaaccagtcg gaaatcaaca
    15061 aattaatcgg caaaaccgaa cgtgccggct acaccatcgt gccgctggat ttgcattttt
    15121 cgcgcggaaa aatcaagatg gaaatcggtt tggcaaaagg taagaaacag cacgacaaac
    15181 gtcagagtat gaaagaggcg gattggaaac gcgagaaaca gcggttgatt aagcatacgc
    15241 gctgattgtg gaaacagtgc ttcagacggt atgggcaagg ctgccgcctg atgtgtaaac
    15301 gttctacacc taaaccagca tccgctttaa tcagcacagc atgatgcccc aaaccttcat
    15361 caaaggtttg agcaatttga tgataaattg gcattaacca agggtaaagt gcggtcattt
    15421 ttcctgaaat ttttgtaaat aacgagggca attatacaca ttttcaaact ggcttgacaa
    15481 aatagcaaaa agcaaacgtt tacctttaat aaataagaaa taaatagaga aaaatgtgag
    15541 ctaaatgaca ttttttgttc agttttatgc taaaatccac gcccttattt atttttactt
    15601 gacaaaggaa aatttattat gaaactcgaa gcaagcaagc aagcaagcag aagtttaaaa
    15661 aatcatttat tataagtcta tttttttcta ttctttatac ctctccgctt ttggctgttg
    15721 attacgttta cgacaaaacc aagctcactg atgatgaaat tacccgctta aaaaaactcc
    15781 gcgatagaaa tagtgaatat tggaaagatg atttgttcag gattgatata ccaaaagaaa
    15841 ctggtctccg tcatgacatt gagggggcag caaaaggaaa tttttcgtat ccaatttttt
    15901 atatcccaac tgatgataaa cctattgatc ctgacaaaaa gatctctttt ttcgacagcc
    15961 cttatacccc tggctacacg gctgctttcg tgcagggatt tggcgttaaa gaaagaaatg
    16021 gctatacgga agaacaagct aaaaaatata tcgatgaatt gagaacaaaa ttaaaaaccg
    16081 ctcctataat cgcagccgcc cagctgggat attttcaatt aaatgatgga agcggcgata
    16141 gctggctgta taattattat cgcaatgata ttttcaattc ttatggagag ataaaacata
    16201 gcaatattaa cagtgaaatt cttgctgttg gtaatgttgc tgatttgtat tataaacact
    16261 tcataagaaa tccatttata tatgtagatt ggtctatatt ctctctcccc cttcctagat
    16321 taaaagtttc cgaaaattcc cacgttattg ggcaaattat tcatctttac cgattagact
    16381 tagaggattc tctttgggag cctcgttggg attctgatgt tagttattta aatttattca
    16441 atagccatat tcgttttaat accaaaaatg acagccttgt tgtaggcgaa agccgcatta
    16501 gaccaacccc cgataatgcc ttgaataccg aaaaatattt aaaaagccaa tttagcgata
    16561 caggttatca tagttccgtg agaagcactt atataggtta tccaagtatt cgctttgctt
    16621 atgatttaag cgaaagagag gctgacaagc ccgtattaac gttaaaaagc aaggtaacag
    16681 ggaaaacagc tattgttttt gaagaaaaag ccttaaacaa tttaaaaaac ctgacttacc
    16741 gacagctcat caaaacagaa accgatgttg aaccagatgc gttttttctt ttagaagaat
    16801 ataaaaaagg cagatatcgt ttatttttgc gacaatgtcc aaacggtttt tgtatcggtg
    16861 tagaaaaact agccattcca actcatcttg tcgcctccta tgcacaacag gctcaagccg
    16921 ccaatacttt attcaaccta cgtttgaacg acaaaaacag cgatattttc gaccgcactt
    16981 taccacgcaa aggcttgtgg ttgcgtgtga ttgacggaca ttccaaccaa tgggtgcaag
    17041 gcaaaacagc acctgtagaa ggctatcgta aaggtgtgca actcggcggc gaagtattca
    17101 catggcaaaa cgaaagtaat caactttccg ttggcttaat gagcggacaa gcagaacaac
    17161 gtagcacttt ccgcaatcca gatacagaca atcttacaac gggaaatgtg aaaggctttg
    17221 gtgcaggcgt ttacgccact tggcatcagc ttcaggacaa acagacaggt gcgtatgcgg
    17281 atagctgggt acaatatcaa cgcttccgcc accgcattaa cactgaagat gctacagaac
    17341 gttttacttc aaaaggtatt accgcctcaa ttgaagcggg ttacaatgct ttattggcgg
    17401 aacacttcac taaaaagggc aacagccttc gtgtttacct acaaccacag gcacaattga
    17461 cttatttggg ggtaaacgga aaattcagcg atagcgaaaa tgcccacgtg aatttacttg
    17521 gctcccgcca attacaaact cgggttggcg ttcaagctaa agcgcaattt tcgctctata
    17581 aaaatatcgc tattgaacct ttcgccgctg tgaacgcgct ttaccacaac aaacctttcg
    17641 gtgtggaaat agacggagag cgtcgagtga taaacaataa gacggcaatt gagagccaat
    17701 taggtgtagc agtgaaaatc aaatctcact taactttaca agcaacattc aaccgccaaa
    17761 caggcaaaca tcatcaggct aaacaaggtg cgttgaattt acagtggacg ttttaagtta
    17821 agttgtaaca aaataaaatc ggcagagaaa tttactgccg atttttttat tttaaaaatg
    17881 cggtcatttt tcgtgtcatt tccaccaatt ttttaccgca ctcacgagct cgcgctaaac
    17941 cgacaatcgg atctattgtc cctttctttg ttgtgtatgc aaatcagaaa tgccggttac
    18001 ggaagcagtt tgcccggatt cataatgtta tatggatcaa ggtgttgttt gatgcttttc
    18061 atcagggcga tttcggcagg cgtgcgtact ttgtccagcc actgtttttt gatgatacct
    18121 atgccgtgtt cggcggcaat cgtgccgttg caggcaagaa cgttgcgata aactgcgtcg
    18181 ttgatgtcgt tttcgtaacg atagacttca ttgctgagga tttcgggcag gaaagtattg
    18241 tagtgcaggc tgccgtcgcc aagatgtccg aagcagacga tttgtatgcc tttaaaattc
    18301 tgttccaaat ctttggcgca ccggcggaca aagtcggcaa cgcgtccgat aggaatggca
    18361 atatcgtgtt tgatgctggt gcccagtttg cgttgcgatg cggagatgtt ttcgcgcaat
    18421 gcccacatat ggatacgttc ttgttcgctt tgtgccaata cgctgtcggt aaatcctttt
    18481 ttataaagaa attcgacaag ccgatcatca agattgctgt cgggtaatga gtcggtcaac
    18541 tcaagtaaaa tatgccattc tgaatgtgtc ggcaggggga gtttgctgaa ttcggaagac
    18601 aactcggcgg caaaacggcc gatcagctca aaactgcata ggcgttcggc aaagtgagct
    18661 tgggtttcgg tcagcaggcg gacggcggat tcgatgtcgg gaatgccgac ccatgccgtt
    18721 gccttgtccg agggacgggc aaaaagcttg agcgtggcgg cagtgatgat gcccagtgtg
    18781 ccttcgctgc cgataaacag atggcgcagg tcgtagccgg tggtgttttt atgcagggga
    18841 tggagatggg aaaccagttc gccgttgggg aggacgactt ccaaaccgat aaccaggtcg
    18901 cgcatcgtgc cgtaacgcaa tacgttcaaa cctccggcat tgcaggcgat gttgccgccg
    18961 atttgacagg agccttcgct ggcgagactg agtgggaaca gcctgtttga ggcttcggct
    19021 gcctgttgga cggtttggag tacggaacct gcttcgacgg ttatgcagtt gtctgacaaa
    19081 ttgatgctgc ggatgcggtt gagtttggaa aggttcagca atacgctgtt ttccgatact
    19141 gccgcgccgc acaaaccggt attgccgcct tgcggcgtaa ccggaatgcg gtgttcgtaa
    19201 caaaaacgca taatggtttg cacgctttca acgctgcgcg gctgcaaaat gatgtctggt
    19261 gcagacgtaa agcggttgcg ctggtctttt aaaagcgtcg gagaaagttc ggcaatttca
    19321 tctgccggca ggagtcggga aaattcggta tgaaggtcag gcatgtttgt cggaagggta
    19381 gggagaagat tgaggaaggg aatctgatgc cgtctgaaaa atcaaaaaag caggatactg
    19441 aacgtatcct gcttttttgc aagcggaaaa tgcttatttt gcagcttcgg cagcaggagc
    19501 ttcagcggca ggtgcttcag cagcgggagc ttcagcagcg ggagcttcgg tagcggcagc
    19561 ttcagcagtg ggagcttcgg cggcaggtgc ttcagcagcg ggagcttcag cagcgggagc
    19621 ttcagcggca ggtgcttcag tagcggcagc ttcagcagcg ggagcttcgg cggcaggtgc
    19681 ttcagtagcg ggagcttcag cggcaggtgc ttcggtagcg ggagcttcag cggctttttc
    19741 accgccgcag gctgccagga ccaaagacaa caaagcagcg gcaaacaggg attttttcat
    19801 tttagtaacc tttagaacca atttaaatta ttgaaacaaa taggtcttta tcgaccatac
    19861 tacaaaccgg gttggaaatt catcgctgaa tttgcgtaat tatgcactat ggaataaggg
    19921 attgcaatgt cgtacagatg ggaaaaaggt ttgaatgaaa agttttctgg cgggcggatt
    19981 gataaatcct gtaaaacggt tttgctgtta aaaattcgat agggataaat attatagaga
    20041 ttaatataat ttcagtatct tgtatgattt attaaggcgg attgggaaag tcggcagaca
    20101 ggtcggaatg gttttaataa tgttgtaaaa aagttacatc gcaataaaat aacaaaaatt
    20161 tacattgaaa tatgtgctgt acagtttatg aacaggttaa cggtaatcgg ccggcgcggc
    20221 ggttgtgtta gaatgcgcct gtaatcaatc tgctgttcct gaaaggacaa atatggctat
    20281 cgtgaagaat tccgtggttt cgctgcatta tgagatgtat gatgccaaca atcagctttt
    20341 ggacaaaacc gaagaaccga ttgcgtatct gcacggcggt tacgacggca ttttcccttt
    20401 ggtggaagag gcgttgcacg gtaaggatgc cggcgatacg gtcgatgtgg cgctttcgcc
    20461 cgacgatgcg ttcggcgagc aggattcgga gttggttcgt attgaagatg tgggcgtgtt
    20521 ccctgttgaa gtcgaagtcg gcatgatgtt tgaagccgac gatcctgaaa ccggcgatgt
    20581 tgtcgtctat cgtgtaaccg atgttgccga cggcaaggcg gtggtggacg gcaaccatcc
    20641 tttggcaggc atgaaaatcc gttttaaggc tacggttgaa agcgtgcgcg atgcatccga
    20701 tgaggaaatc gcacacggtc atgtccacgg cccgcacggt catcaccacc actgattgcc
    20761 ggcagcttgg aggtatctga tgccgtctga aaagggtttg ttccgtttca gacggcattt
    20821 tgtttgatgg atggagaagg tatgtcggtt gggcattatg aaaattttcc cgtcggttcg
    20881 ctgattttgc cgcgcaggtt gaggaagccg gttcatgcgg tgtatgcgtt tgcacggacg
    20941 gcggacgata tggcggacga gggcagtatg ccgtctgaag ccaggttggc ggggttggat
    21001 gctttgcggc gcgagttgga cgtgttggca tcgggcggcc ggtcggcgca tcctttgatt
    21061 gcacgattgg atgccgaggc ggttgtgccg ttcggtttgg atttgcagcc gttttatgat
    21121 ttgctttcgg cgttttcgca ggacgtggtt aaaacgcggt acgcgcattt cggcgaactg
    21181 gccgattact gccggcgttc cgccaaccct gtcggacgca ttatgctggc tttgtacggg
    21241 aaaacggatg cggtgtgcgt ggcgcaaagc gacggcatct gtacggcttt gcaactggtg
    21301 aatttttggc aggatgtggc tgtggattgg caaaagggca gggtttatat tccgcaggac
    21361 gatttgttga aattcggtgt ttccgaagga cagattgcgg agggtagggc ggatgcggcg
    21421 tttcagcggc tgatggcgta tgagtgccgg cgcgcatttc gtatgctgaa ggcgggttcg
    21481 cctttagcgc gcgaattgaa agggcgtatc ggtttggaac tccgtatgat tgtgttgggg
    21541 gcgcagttga ttttgcagaa actggacgcg tgccgatacg atgtgtttgc acaacgcccc
    21601 gttttggata agaaggattg gttgattatg ctgaaacgcg cgttgtggaa atgatggggg
    21661 aaatgcatgc cgtctgaaag gggttgttcc gcttcagatg gcattttgca ggcggttggt
    21721 gtggtcaggc gatttcatgc gggttcaacc tggcttgatc ttctgcaccc aagccgatga
    21781 gtttgcgcag tcgggtcatg tagaaattga catccaaacc ggtgccgtag cgttgcgccg
    21841 tccacagtgt ttccgccagt gcctccatca tttcgtgttc ggcttccagc cagccgcgtt
    21901 tggcgcacag ggtgtcgtgg attgcgcgta tgccgtgcgg ctggtctatg cccgcctgtt
    21961 cttggacgga caaatgcagc gacatatgca ggaaggggtt gctttcgccg ttttcgggta
    22021 gccagtcggt gtccagatgg tcttcgatgc gttcgagata acggtggtat tcgggatggg
    22081 cttcgataat gcggagggct ttctgttcca gtacgctcag ttgcagcgga ttgagccgct
    22141 gctgccacac gcgggcgaaa aagcggcgga catcgtgggt attgacatcg tacatggcgg
    22201 gttcggtaag atgtttatgg aaataggttc ggataatggc gggttttaca taaattaaat
    22261 cccaccgtca accttaccat ttataatgca gacaaattat ttgtctgaaa cggattcgat
    22321 tatgaaaaaa ctgatttgcc tgttcgccgt atttttgatg ttgtgcggac gagctttcgc
    22381 gctggatgcg aacgatctgc tgccgccgga aaaggcattc gtgccggagc ttgccgttgc
    22441 cgacgacggt gtgaacgtcc gtttcaggat tgccgacgga tactatatgt atcaggcgaa
    22501 aatcgtcggc aagaccgatc cggcggattt gttgggacag ccttctttca gcaagggcga
    22561 agagaaggaa gacgagtttt tcggcaggca gacggtttac catcacgaag cgcaggttgc
    22621 ctttccttat gcaaaggctg tcggcgaacc gtataaattg gttttgacct atcagggctg
    22681 tgccgaagcc ggcgtgtgct atccgcccgt ggataccgag tttgatattt ccggcaacgg
    22741 cacttaccat ccgcaaaccg acgaaccggc atccgccaaa gaccgctttt tgcagccttc
    22801 ctctcaaaac ggcagcgggg cgttgccgcc cccgaagggg gatgagggcg gcgacagccg
    22861 tttcaagctg tcttgggata cgctcaacgc caatcttttg gcgttttttc tcgccggttt
    22921 gggtctgagt tttaccgcct gtatgtatcc cctgttgccg attgtttcca gtattgtggt
    22981 cggcgacaaa aaggcgggca aggcgcgggc gtttgtgctg tccgtcgttt atgttcaggg
    23041 tttggctctg acttatacgc tggtcggcat tgttgccgga ctgacgggcg cgctgctgac
    23101 cgtatggttg cagcaggctt gggtggtgtt ggcggcatcg gctttaatgg tcgtcttggc
    23161 actgtctatg ttcgggctgt tcaacatcca gcttcccaac gccgtgcagt cgtatttcca
    23221 aaaccagagc agcaggcttt caggcggtaa aatcgtttcc gtctttatta tgggcatatt
    23281 gtccgcgctg attgtcgggc cgtgcgtcgc cccgccgctg gcatttgctt tgggctacat
    23341 cggtcagacg ggcgatgcgg ttttaggcgg tttggcactt tacactttgg cgttgggcac
    23401 cggcgttccg ctgattgcca tcggcacgtt cggcgggcat atcctgccta gggcaggcga
    23461 ttggatgaat gccgtcaaat acgctttcgg ctttatcctg cttgccgtcg ccgtttacct
    23521 cgccacgccg cacttgccct attatctcgt cgtcgcgctg tacacgctgc tgatgctggt
    23581 tcctgcctgt atgctgctgg tcaacggacg caggcagaaa cgccgtccga aagctgtggc
    23641 attcgcattg ggcggcatat tgctgatagg cggcgcgtgg ttcggctggc agggcgcaaa
    23701 cggcaaaacg accgcgctgc accatttcct gaccctcaat ccaccagccg aagcaggcaa
    23761 atcttcggaa cacggcaaaa tgtttgccga tactgccgcg ctgaaggcag cgatggatac
    23821 ggcgttgaaa gaacatcccg acaaacccgt cgttttggat ttttatgccg actggtgtat
    23881 ttcctgcaaa gaaatggcgg catacacgct caatcagccg gaagtgcatc aggcggttga
    23941 tatggaacgc tttttccaaa tcgacgtaac cgccaacacg cctgaacatc aggcgttgtt
    24001 gaaagaatac ggtctgttcg ggccgccggg cgtgtttgtc gtccgctccg acggcagccg
    24061 cagcgagccg ctgctgggtt ttgtcaaagc agacaagttt atcgagtggt atgaacaaaa
    24121 ccgctgattc cgatgtttca aatgccgtct gaagttctca gacggcattt ttgtcagaaa
    24181 ggaaaacggc aatatccact atacacacgg aagcagtcta aagatgtgaa aaccgtgtgt
    24241 ataagttagt ttgcgtattg aaattaaaag ataaaaatac gatgattaaa aaataggcat
    24301 aagaaaagat aaacacagcc ggtgtttgcc ggtgtgtgta tcttttgtgg aaaaacatct
    24361 taatgtcatg tattttatgg aaaataataa catgattaaa aaatagtcaa tcattatagt
    24421 ggattaaatt taaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
    24481 ggcgaggcaa cgccgtactg atttaaattt aatccactat aaggttcagg taagcggctg
    24541 caaccgtcaa tgtctgaaaa gaggcagtgc tgtttaagca tggaaagccg gataaaaaaa
    24601 actgcctccg tttccggagg cagttcgtgt gcggataaaa ccttacagac cgctcaaacg
    24661 ggcagtgtcg tgggcaatca tcagctcttc gttggtcgga atgaccacgg caacggcttt
    24721 gctgtcggca gtggtaatca cgccggcgtt gccgaagcgg gctttcaggt tggcttcttg
    24781 gtcgatgttc agaccgagga agcccaagta gccgatcacg cgttcgcgga tgatgtcgga
    24841 gttttcgccg atgccgccgg taaagaccag tgcgtccaaa ccgcctgcgg caaccgccat
    24901 actgccgatg tatttggcaa ggcggtagat aaacatatcc aaggccaatt tcgcgccttt
    24961 atgccccttg gcggcttctt cttcaatggt gcggcagtcg ttggacaggc cggaaatgcc
    25021 gagcagaccg gattttttgt tcagcatttc agtgatttgg gcgatggtca tattggcgtt
    25081 ttcggcgaga aagccgaata cggaaggatc gatgtcgccg ctgcgcgtac ccattaccag
    25141 cccttccagc ggggtcaggc ccatactggt gtcgcgcgat tcgccgttgg cgacggcggt
    25201 aatggacgcg ccgttgccca agtgggcaat gaccatacgc aggtcttttt tgtctttgcc
    25261 gaggaagcgc gcggtttcgt cggcgacgaa gcggtagctg gtaccgtgcg cgccgtaacg
    25321 gcgcaggccg tatttttcat acaactcctg cggaacggcg tatttgtagg cgacttcggg
    25381 catggtttgg tggaaggagg tatcgaatac gacgacgttg ggcaggtctt tgaaaatgct
    25441 ttgcgcggca cgcaggccca agaggtgggc ggggttgtgc aggggggcga gcgggatgca
    25501 tttttcgatg ccggcaatga cttcgtcgtc aacgaggatg gattcgctgt acagttcgcc
    25561 gccgctgacg acgcggtggc cgatggcgcc gatgcggctg tcgaggccgt gggctttgag
    25621 ttcttccatc agggcttcga ccgcgccggt gtggtcggga tgtgcggaca gatcgacttt
    25681 gtgtttttcg ccgtttactt tgaatgtgat gtaggcatcg ggcaggttga gtttttcggc
    25741 aaggcagctg agcaggactt cgccgctgcc gttatccagg accgcgcctt tgagggacga
    25801 gctgccgcag ttcaaaacca agatcaattt ttgggacatt ttcttactcc ggaaagtttc
    25861 agacggcatt ggaatcggac acggatacta accggattcg tgccgaatcc gttttgcctt
    25921 cctaggcggg aaagtagtgg ggccgtctga aacggttgat aaaagaagca ggctattcta
    25981 acaaaaaatc tttgcaattg cttggcttaa tcgggcgttt gcgtgaaaat ggcggaagtc
    26041 gggtattttg acggaaaaaa gggggatatg cggtttgccg gtgttttcgg ctgggggagg
    26101 gagaatttct ttgcattctt cccgtccaaa ctggtacatt cgccctgttt ttagttggtg
    26161 tcgtctgaaa aaaggcgggt gggaaattta tgcattattt cagtattcat gaccaatcgg
    26221 gacggcatat aggctttttc atcctgcttg ccgacgacga atcggaagca cggccgcaaa
    26281 gcgggcgttt tgccgtcaaa cttgaaggcg gaatggaaaa tcatgtcctg tcgccgttcg
    26341 gtcagacgga aattccccaa tattggcgcg tggtcaaaga ccgtatcgag ctgttttttg
    26401 acgaagctcc cgtcggaact ttgcgtaacg aatatctgac cgtgtcgggg cagacttttg
    26461 ttttgaacga tttgacaggg aatatgtgat ggaaagcatg tttatattgg tgcccatcag
    26521 cattattttg gcgtttgtca tcggctggtt tttttggtgg tcgggcaaaa acgggcagtt
    26581 tgacgacttg gaagggccgg cgcaccgcat cctgatggat gatgattcga caagtaaact
    26641 gattgaaaaa gagagggaaa acgatgtacg ctaaaaaagg cggtttggga ctggttaaaa
    26701 gccgccgttt cgcacctctt ttcgctacgc agtttctcgg cgcgttcaac gacaatgtgt
    26761 tcaaaaccgc gctgtttgtg atgattgggt tttacggttt ggggcaaaac ggcttcctgc
    26821 ctgccggaca gatgttgaac ttgggcgcgt tgttgtttat tttgccgtat ttcctgtttt
    26881 cctcgctgtc ggggcagttg ggtaacaaat tcgacaaggc cgttttggcg cgttgggtca
    26941 aggtgctgga aatgatcatt atggcggtgg cggcatacgg gttttatatc cggtctgccc
    27001 cgctgctttt ggcgtgtctg ttttgcatgg gcgcgcaatc gacgctgttc gggccgctga
    27061 aatacgccat cctgcccgat tatctcgacg acaaagagtt gatgatgggc aacagcctga
    27121 ttgaatcggg tacgtttgtc gccatcctgt tcggtcagat tttgggaacg gcggtggcag
    27181 gtgtaccgcc ttatattgtc gggatactgg ttttgctggt cgccgtcgga ggcacggtcg
    27241 gcagcctgtt tatgccgtcc gtccccgcca aggctgccga tacccaaatt gagtggaata
    27301 ttgtccgtgg cacaaaatcc ctgcttcgtg aaacggtgcg gtacaaaccc gtctttaccg
    27361 ctattatcgg tatttcgtgg ttttggtttg tcggcgcggt ctatactacg caactgccga
    27421 cctttaccca aatccatttg ggcggcaacg acaatgtttt caacctgatg cttgccctgt
    27481 tttccatcgg tattgccgcc ggttcggtac tgtgtgccaa gttcagcagg gaacggctga
    27541 tgttggcttg ggtaacggtt ggtgcgttgg gtttgacggt ttgcggcctg gttttggtgt
    27601 ggctgacgca cggacaccgt tttgaagggc tgaacggcat tttttggttt ttatcgcaag
    27661 gatgggcata tcccgtgatg gcggtgatga cgctgatcgg ctttttcggc ggatttttct
    27721 ccgttccgct ctatacttgg ctgcaaaccg ccagtagcga gactttccgc gcccgcgccg
    27781 ttgccgccaa caatatcgtt aacggtattt ttatggtttc cgccgccgtt ttgagcgcgg
    27841 tgttgctgtt tttgtttgac agcatttcct tgttgtatct gattgtcgct ttgggcaata
    27901 ttccgttgtc ggtatttttg attaagcgcg aaaggcggtt tttaggcgcg gcggcaatca
    27961 ggaaaaaacc ttgaatgccg tctgaaaaaa ttgtccccaa aatacacaat ccgcctgccc
    28021 aatgttgtga acacactgtg aataagtggg tttgtgcatt gaaatgaaaa cagaaaaata
    28081 tattgcttaa aaaatagtca gcagatggaa aatttgaaga aagaagcaga catgattcaa
    28141 cgccaaatca tcctcgatac cgaaaccacc ggtctgtatg ccgacggcgg cgaccgcttg
    28201 gtcgagtttg ccggtttaga aatggtcaac cgccaaatga ccgacaaaaa cctgcacctc
    28261 tatgtccacc ccgagcgcga tatgcccgaa gaggcggcga gggtgcacgg tctgacgata
    28321 gaggttttgg aagggaaaaa cgcgccgccg tttgcagagg tcggcaggca gattgccgat
    28381 ttcctgcgcg gtgcggaact gattatccac aatgccaaat tcgacgtggg cttcctcaat
    28441 atggagttcc gccggatggg gctgccgact gtcgaggaac tcggctgtac cgttaccgat
    28501 accctggcga tggcgcgcga aatgttcccc gggcagaaag ccagcctgga tgccttgtgc
    28561 aaccgttttt ccgtcgaccg cagcaagcgc gtcctgcacg gcgcattgat cgactgcgaa
    28621 cttttgggca aagtctatct tgctatgacg cggcggcagt tcgacctgat gggggaggcc
    28681 gaggaggaag agccggcagc caaacctgtt gcatctgccg aaatgaaact cggtgccaaa
    28741 ctgaaagtga ttaaggcgga tgaagccgaa ctggcggcgc acgggcaata tttggacggc
    28801 ttgggcgaag cctgtatctg gcgcaaggaa gccgtgccgt cggaaaacgg cggggcggac
    28861 gcatgaagcg caaaaatatc gcgctgattc ccgccgccgg catcggggcg cgtttcggtg
    28921 cggacaaacc taagcagtat gtcgaaatcg gaagcaaaac cgttttagaa catacgattg
    28981 ggatttttga acggcatgag ggcgtcgatt tgaccgtcgt tgtcgtctcg cccgaagaca
    29041 cgtttgccga taaggttcag acggcatttc cacaggttcg ggtgtggaaa aacggcggac
    29101 agacccgcgc cgaaaccgtc cgaaacggtg tggcaaaact gttggaaatc ggtttggcgg
    29161 cggaaaccga caatattctg gtacacgatg ccgcgcgttg ctgcctgccg tctgaagctt
    29221 tgacgcggtt gatagaacag gcgggcaacg ctgccgaagg tgggattttg gcaattcccg
    29281 ttgccgatac gctcaagcgc gcggacggtg ggaacattag tgcaaccgtc gagcggacga
    29341 gcctttggca ggcacaaacg ccgcagcttt tccgcgccgg gctgctgcac cgcgcattgg
    29401 ctgcggaaaa cttggacggc attaccgatg aagcgtccgc cgtcgaaaaa ttgggcatcc
    29461 gccctttgct ggtgcagggc gacgcgcgca atttgaaact gacgcagccg caggacgcat
    29521 acatcgtcag gctgctgctc gatgccgtct gaacttcaat tcaaaccatc aggaacacaa
    29581 acgatgacga acatccgtat cggacagggc tacgatgtcc accaactgac cgaaggcaga
    29641 aagctgatac ttggcggagt tgaaatccca tttgaaaaag ggctgctcgg acactccgat
    29701 gccgatgcgc tgctgcacgc cgttaccgac gcgctgcttg gcgcggcagg tttgggcgat
    29761 atcggcagcc atttccccga taccgcctca gagtttaaag atgcggacag ccgcgccctg
    29821 ctgcgcgatg cctatcggag cgttcaagct aaggggtggc atgtcgtcaa tgtcgatacg
    29881 accgtgattg cacagaaacc caaactcgcg ccgcacattc cgcaaatgcg tgccaacatc
    29941 gccgccgatt tgggcataga catttcgtgc gtcaacatca aaggtaaaac caacgaaaaa
    30001 ctcggctatc tcggacggat ggaaggcata gaggcgcagg cggcggtatt gctggtacgg
    30061 atttgaacgc ggcgggcagg acgtgcaaaa aaatgttaag atgacggctg tttgttgata
    30121 cggtttccca aaggacaaaa atatgacgac acaagacgaa ctcaagcgca tcgccgccga
    30181 aaaagcagtc gaattcgtac ccgaaaacga atacatcggc atcggtaccg gttcgaccat
    30241 caactttttc atcgaagcct tgggtaagag cggcaaaaaa atcaaaggcg cggtatctac
    30301 ttccaaaaaa tccagtgaac tgcttgccca atatgatatt cccgtcgttt ccctgaacga
    30361 agtatcgggg ttggcggtct atattgacgg tgcggatgaa gtgaaccacg ccctgcaaat
    30421 gattaaaggc ggcggcggcg cacacctcaa cgaaaaaatc gtcgccagcg catccgaaaa
    30481 attcgtctgc attgcggatg aaagcaaata tgtttcacgt ttgggcaaat tccctctgcc
    30541 tgtggaagtc gtcgaaagcg cgcgctctct tgtttcgcgc aaactgctcg ctatgggcgg
    30601 acagcccgaa ctgcgtatcg gctacaccac gttttacggc aaccaaatcg tcgatgtcca
    30661 cggcttgaat atcgatcagc cgctgacgat ggaagacgaa atcaacaaaa tcaccggcgt
    30721 actcgaaaac ggaatattcg cccgcgatgc cgccgatgtg ttgattttgg ggacggaaga
    30781 gggtgcgaaa gtcatctatc cctgccaagg gtaatcctga cgttaagatg ccgtctgaaa
    30841 tccggttcag agatgttcag acggcatcgg catttgaaaa atatgcagat taaaaaaatc
    30901 atgaaatggc ttcccgtcgc cctgtcgctt ttgggcgcgt tgggttatac gggatacggc
    30961 agcgaggcgg tgcggacggc ggttgccgta ctcgacgtac tcggcgcggc aggggacgcg
    31021 ggttccgacg cgcccgctcg ccgccgagca tcggcgaaat ccggccaccg ctacacaggc
    31081 acggtgtcca aagtctatga cggcgacacc cttcacgtta tcgacggcga cggcgcgaaa
    31141 cacaaaatcc ggatggcgta tatcgacgcg ccggagatga aacaggctta cggcacgcgt
    31201 tcgcgcgaca acctgcgtgc ggcggcggaa ggcaggaaag tcagcgtccg cgtgttcgac
    31261 accgaccgct accagcgcga agtggcgcag gtttctgtcg gcaaaaccga tttgaacctg
    31321 atgcaggtgc aggacggggc ggcgtggcat tataaaagtt atgctaaaga acagcaggat
    31381 aaggcggatt ttgccgatta tgccgacgct caaattcagg cggaaaggga acgcaaagga
    31441 ttgtggaaag ctaaaaatcc gcaagcgccg tgggcgtacc gccgggcagg caggagcggc
    31501 gggggcaata aggattggac ggattccgtg ggcgaatggt tgggcatttg gtaaagacac
    31561 cgcacggctt tgaacggcaa atgccgtctg aaaaccgcgc gccctgcatt tttgcggaga
    31621 cgcgcaaccg ccgtctgccg cgataaaaaa gttcaggcgg cggtttttac ggtaaaatgg
    31681 cggattattt tgcaacggcg gcagttccgc cggtttttga tacggacggt ttatggattt
    31741 tcgttttgac attatttacg aataccgctg gatgtttctt tacggcgcac tgacgacctt
    31801 ggggctgacg gtcgtggcga cggcgggcgg ttcggtattg ggtctgttgt tggcgttggc
    31861 gcgcctgatt cacttggaaa aagccggtgc gccgatgcgc gtgctggcgt gggcgttgcg
    31921 taaggtttcg ctgctgtatg ttacgctgtt ccggggtacg ccgctgtttg tgcagattgt
    31981 gatttgggcg tatgtgtggt ttccgttttt cgtccatcct tcaggcggca ttttggtcag
    32041 cggcgaggcg gcaatcgcgc tgcgtcgcgg atacgggccg ctgattgccg gttctttggc
    32101 actgatcgcc aactcggggg cgtatatctg tgagattttc cgcgcgggca tccagtctat
    32161 agacaaagga cagatggagg cggcgcgttc tttggggctg acctatccgc aggcgatgcg
    32221 ctatgtgatt ctgccgcagg cattgcgccg catgctgccg cctttggcga gcgagttcat
    32281 cacgctcttg aaagacagct cgctgctgtc ggtcattgct gtggcggagt tggcgtatgt
    32341 tcagaatacg attacgggcc ggtattcggt ttatgaagaa ccgctttaca ccgtcgccct
    32401 gatttatctg ttgatgacga ctttcttagg ctggatattc ctgcgtttgg aaaaacgtta
    32461 caatccgcaa caccgctgat tggagtcata tcaacaaaaa gctgcccggg tattcatttg
    32521 aggcagcttt tttgtggcaa atttatcgtg gattaacaaa aaccagtacg gcgttgcctc
    32581 gccttagctc aaagagaacg attctcta
//
LOCUS       DAWHXS010000009        31341 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWHXS010000009 DAWHXS010000000
VERSION     DAWHXS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.009"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..746)
                     /locus_tag="WIE22_000332"
     CDS             complement(<1..746)
                     /locus_tag="WIE22_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ4126477.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDT"
     gene            complement(887..3445)
                     /gene="glnD"
                     /locus_tag="WIE22_000333"
     CDS             complement(887..3445)
                     /gene="glnD"
                     /locus_tag="WIE22_000333"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ4126478.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGISDARQFAADHF
                     LTEEESDLLAWLVENHLLMSIVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLAGNGGNPHALFGRRQQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETSNHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEIFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(3494..3814)
                     /locus_tag="WIE22_000334"
     CDS             complement(3494..3814)
                     /locus_tag="WIE22_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4126479.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(4138..4611)
                     /gene="bfr"
                     /locus_tag="WIE22_000335"
     CDS             complement(4138..4611)
                     /gene="bfr"
                     /locus_tag="WIE22_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ4126480.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(4639..5103)
                     /gene="bfr"
                     /locus_tag="WIE22_000336"
     CDS             complement(4639..5103)
                     /gene="bfr"
                     /locus_tag="WIE22_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ4126481.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(5567..6550)
                     /gene="lipA"
                     /locus_tag="WIE22_000337"
     CDS             complement(5567..6550)
                     /gene="lipA"
                     /locus_tag="WIE22_000337"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ4126482.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(6543..7166)
                     /gene="lipB"
                     /locus_tag="WIE22_000338"
     CDS             complement(6543..7166)
                     /gene="lipB"
                     /locus_tag="WIE22_000338"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ4126483.1"
                     /translation="MKVIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(7168..7443)
                     /locus_tag="WIE22_000339"
     CDS             complement(7168..7443)
                     /locus_tag="WIE22_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ4126484.1"
                     /translation="MTEQKNKTSLIEFPCTFSLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQIKVENQEQLDNIYRALTSHELVKVVL"
     gene            7628..8698
                     /locus_tag="WIE22_000340"
     CDS             7628..8698
                     /locus_tag="WIE22_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ4126485.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLAFALGDTLAPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNTLKAWFPVLMRQGSNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(8895..9842)
                     /locus_tag="WIE22_000341"
     CDS             complement(8895..9842)
                     /locus_tag="WIE22_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ4126486.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(9857..10264)
                     /locus_tag="WIE22_000342"
     CDS             complement(9857..10264)
                     /locus_tag="WIE22_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ4126487.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIAKP"
     gene            10345..11004
                     /gene="ung"
                     /locus_tag="WIE22_000343"
     CDS             10345..11004
                     /gene="ung"
                     /locus_tag="WIE22_000343"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ4126488.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            complement(11512..12027)
                     /locus_tag="WIE22_000344"
     CDS             complement(11512..12027)
                     /locus_tag="WIE22_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126489.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(12493..14415)
                     /locus_tag="WIE22_000345"
     CDS             complement(12493..14415)
                     /locus_tag="WIE22_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4126490.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKFTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(14492..14887)
                     /locus_tag="WIE22_000346"
     CDS             complement(14492..14887)
                     /locus_tag="WIE22_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ4126491.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            15060..16367
                     /locus_tag="WIE22_000347"
     CDS             15060..16367
                     /locus_tag="WIE22_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ4126492.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            16360..16794
                     /locus_tag="WIE22_000348"
     CDS             16360..16794
                     /locus_tag="WIE22_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126493.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(16853..17122)
                     /locus_tag="WIE22_000349"
     CDS             complement(16853..17122)
                     /locus_tag="WIE22_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ4126494.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(17304..19766)
                     /gene="lon"
                     /locus_tag="WIE22_000350"
     CDS             complement(17304..19766)
                     /gene="lon"
                     /locus_tag="WIE22_000350"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ4126495.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            19851..20042
                     /locus_tag="WIE22_000351"
     CDS             19851..20042
                     /locus_tag="WIE22_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ4126496.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            20017..21120
                     /locus_tag="WIE22_000352"
     CDS             20017..21120
                     /locus_tag="WIE22_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4126497.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(21192..22937)
                     /gene="recD"
                     /locus_tag="WIE22_000353"
     CDS             complement(21192..22937)
                     /gene="recD"
                     /locus_tag="WIE22_000353"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ4126498.1"
                     /translation="MERQTDEFAQAAARAAIRFLERYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARTAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRVHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFPDTDGFKKVAVSCLPDFEPAFALTVHKSQGSEY
                     REVWLLPPSAGGSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQG"
     gene            complement(23005..23700)
                     /gene="lolD"
                     /locus_tag="WIE22_000354"
     CDS             complement(23005..23700)
                     /gene="lolD"
                     /locus_tag="WIE22_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ4126499.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVLVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNHHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(23693..24940)
                     /locus_tag="WIE22_000355"
     CDS             complement(23693..24940)
                     /locus_tag="WIE22_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ4126500.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            25145..25423
                     /locus_tag="WIE22_000356"
     CDS             25145..25423
                     /locus_tag="WIE22_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126501.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSDSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(25479..26096)
                     /gene="recR"
                     /locus_tag="WIE22_000357"
     CDS             complement(25479..26096)
                     /gene="recR"
                     /locus_tag="WIE22_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ4126502.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(26163..27701)
                     /locus_tag="WIE22_000358"
     CDS             complement(26163..27701)
                     /locus_tag="WIE22_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ4126503.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKVKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPDDAAARIPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(27761..28135)
                     /locus_tag="WIE22_000359"
     CDS             complement(27761..28135)
                     /locus_tag="WIE22_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ4126504.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            28225..29913
                     /locus_tag="WIE22_000360"
     CDS             28225..29913
                     /locus_tag="WIE22_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ4126505.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVL
                     DVVMMGHTEMWAAMTERDAIYANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNAKMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKRFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            29985..31238
                     /locus_tag="WIE22_000361"
     CDS             29985..31238
                     /locus_tag="WIE22_000361"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ4126506.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVEKLRSKTIIDVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         6811 a         8106 c         8572 g         7852 t
ORIGIN      
        1 gtatcgacga caatcacgtc cacgccggct tcgaccaagg ctttgacgcg ctcttcggta
       61 tcgccgccgg tgccgactgc cgcaccgacg cgcagacggc cttcggagtc tttgttggcg
      121 ttgggaaact cggtggtttt taaaatatct ttaacggtaa tcagaccttt gagttcgtct
      181 ttttcgttca gaaccaaaac gcgctcgact ttgtgcgtgt gcatcagttc gcgcgcttcg
      241 tctatgcttg tgccttcggg gacggtaacc agacgttcgc gcggggtcat aatggcggaa
      301 acgggcaaat cgacgcggtt ttcaaaacgc aggtcgcggt tggttacgat gccgacgact
      361 ttgccgtttt caacgacggg caggccggac attttgcgtt tgcgctgcgc acgcatttcc
      421 aagacttcgc ggatgagcgt cgtcggtgca acggttacgg ggtctttgac cacgccgctt
      481 tcgtggcgtt tcactttgga aacggcgcgc gcctgcattt cgggcggcat gtttttatgg
      541 atgatgccga tgccgccttc ctgtgccatc gaaatggcga ggcgcgcctc ggtaacagtg
      601 tccatcgcag cggaaagcag ggggaggttg agtgtgattt cgcgggtgag cttggtttga
      661 agtttaacgt ctcgcggcag cacggtcgaa tgtgcgggaa ccaacaaaac atcgtcgaaa
      721 gtataggctt tttctacgat acgcataatg ctcggtcttt cagtttgtgc aagatgcacg
      781 gcattatagc acgttaccgg cggcttgaca gtttatattg tttgcaatat cccccgcctt
      841 caggcggcat acggcgtggc ggcggaatgc cgtctgaagg cgggcgttat gataattgtt
      901 ccagcagcgt ctgcttcaat gaggactgga tttttgggtt tttcaggtcg gggctaaaaa
      961 cggtaaaact gtcttcggcg cgttcatcca gtgtggagat tttggcatag cgcaggctga
     1021 cgttgtgggc gaaaaagatt tccgccatat cggcgagcag gaaggggcgg ttgacggcgg
     1081 taatttcgac ggagtaccag tcggggtagt cttcttcggg ggtaatggtg atgcttggcg
     1141 cgatcggcat gtagcggctg cggcggctga tgcggcggct gtggtttgag gtttcggcaa
     1201 cggtgtgtcc gtggataaag ctgttgagtt cggcttcaag cgcgctttga atgtcggggt
     1261 agtcttcggg ggcgtgttgt gagggaattt gcacgatgaa ggtgtcgagg atgtagtcgt
     1321 gttcggtgat aaaggcgcgg gcggcgagga tatcgaagcc gtggcggctg aaaatgcggc
     1381 agagacgggc aaacaggcgc gggccgttgg gcatgaaaac catgacttga aagctgtcgc
     1441 ttttgggcag gatgcggctg cggacgatgg gggtttcaaa gtcgtggact aggttggcgg
     1501 cgtgccacag gatttcgcgg gactggtggc gggcgaagta ggcggaaccg agcgcgttcc
     1561 aaagcttttt ctgctgtttt tcggggacgg cggcgcgggt gagcaagtcg gcggcttctt
     1621 gttggcggcg gccgaagagg gcgtgcgggt tgccaccgtt gcctgcgagg tagcgtccgg
     1681 cggcatggaa gaggctttcc agcaggctgg cgcgccatgc gttccacagc ttgggattgg
     1741 taccgcgtat gtcggaaatg gtcagaaggt agagcgcgct gaggcgttcg tgggtttgga
     1801 cgcgtttgca gaaggcatcg agtacgtcgg ggtcttggat gtcttctttt tgggcgacga
     1861 tggacataag aagatggttt tcgaccagcc aggcaagcag gtcgctttct tcttcggtca
     1921 ggaagtggtc ggcggcaaat tggcgcgcgt ctgagatgcc ttgtatggca tggtcgccgc
     1981 cgcgtccttt ggcgatgtca tggaaaaagg cggcaaggta gaggatgtct tgtttttcaa
     2041 aggactgcat cagtgcagag gcgtagggca gctcgtggct gtgcatatcc aaggcaaggc
     2101 ggcggacgtt gcggacgacg gcgaggatgt ggtcgtccac gggatagatg tggaacaggt
     2161 cgtgctggag caggccgacg attttttccc acgcgggcag gtagcgtccc aatacgccgt
     2221 agaggttgag gaagcgcagg gtttgggtca gtccgttgcc gttgcggaaa aagccggcga
     2281 agcggtggcg gttttcagaa ttttggtaga agctgcggtt gattttgcgc gtcgcgcccc
     2341 accatgcgcg cagggtttgc ggttcgagcg cggtaatgtc gttgcgctgc tgcatgattt
     2401 cgacgatttt gaaaatgtgt tcgggccgtc tgaaaaaaat atcggtgtgc cgcgcggcga
     2461 tttggttgtt gacttggatg tagtcgtcgt caatccgcag ggtaacgcgc aacggggtgg
     2521 aggaaacgcg gctttgcagc ataggcgtga ggatgccgcc cagttgtttg acggttttaa
     2581 tcgcgcggta aaacacgcgc atcagttctt cgctttggcg gcggaggttc aagccttcat
     2641 aacccatgct ttcggcgact tgcggctgca aatcgaacag caggcggtct tcggcgcgct
     2701 tggcgtttaa atgcagatgg atgcggatgt gggcgaggcg gcggtagccg tgcgaaagca
     2761 taccggcttc ggcacgcgtc aaaatccgct gtttgagcag gtcgggcagg tcggtcgcca
     2821 agccttgcgc cttcgctatc caaagcaggg tgtggatatc gcgcagaccg cccggacagc
     2881 ttttgatatt cggctccaat actgcccccg aaccttgcga tttggcgtgg cggtgttcca
     2941 tctccaccag ttttgcctcg acaaacgccg ccacattgcg ctgcgcgttc attttttccg
     3001 ctagtttatc cgccgtttgg cggttgccaa acaaaaacct agcctctaaa aacgctgtgt
     3061 cccccgtaat atcattgcgc acgctttcac atagttcatc aacgctgccg ctttttacag
     3121 acggcatcag tttgcagtcc cacagggttt ggacaaaccg ggcaatctgt tcctgaatgc
     3181 cgtctgaaag cggggcaggg gagacaaccg ccaaatccac atccgaacag ggatacagtt
     3241 cgccgcgtcc gaagccgcct accgccatca ggcataacgc gctgttttga aaatactctg
     3301 cccacaatgc cgccagtaag gtttcgagtg ccgccgtata ttccctgaaa aataccgaca
     3361 cgcggttggc tttcaaataa tgcgcttcgg cggcatcgcg ctgctgtttg aaggtttcca
     3421 gtgctgaaga caggtttgca ggcattttta ttctttcgat tggcgggaaa aagggaggcg
     3481 gatggttcgg cggtcaaata ccgctttcag acggcatttg tcgggtatcg gcggaattgg
     3541 tcatcagctt caaccgttcc tgcggcggca gcagcaactg atacgccgcc acgcccaaag
     3601 ccgccgccat tttttcgata ttcgacaggg cgatgttcca gcgtttgcgc tcgactgccg
     3661 acacataagt cctgtccaaa ccgcattgcc gcgccaattc ttcttgcgac caacccttgt
     3721 tcacgcggaa aagccgcata ttgtatgcca ataccgcccg caaatcctgt tcgtcaggca
     3781 gttcggcagg cagagtcaat ttgttgccca tcatttgctt ccagataaat ggttaaagtt
     3841 aagcatttgc tgttacggat tttacttcac ataaaagcca aaaaaaaggg gtggcggcag
     3901 ccacacccaa acacacacac atcaagagga aagagataaa atcagacaga ttggaaaaca
     3961 gcaaacaagc accctgaatt tcatatcggt attatcaaac aaaaaccctt atcaggtatt
     4021 gattcaaatc aatttagatt ttattcgcaa accgaaaaag aaaaacggca tatccgttta
     4081 tacggatatg ccgtctgatg gtgcggggga ctgctgtatt tggcagtagt ttgtgtttta
     4141 gtcctcttgc gccgctgttt gcaggtagtt gggcagaccg attttgccga taagctcttg
     4201 ctgggtctcc agccagtcta tatgttcttc gttggtgtct ttttgttttt ccaacaaatc
     4261 gcggctgacg taatcctgtt gcgcttctgc tgtggcgatg gcggcaagca gggcttcgtg
     4321 tttttcctgt tctttggtca aatcgcaggc gatgatttct tcggtggact cgccaatcag
     4381 aagcttgccc agttcttgca ggttcggcag accttcgagg aacagaatgc gctcgatcaa
     4441 atcgtcggca gctttcattt caacgatgga ctgtttgaag aaatgttcgc ccagttcttc
     4501 aaagccccag tttttcaaaa tacgggcgtg aaggaaatat tggttgatgg ttaccagcag
     4561 caagcctaag tttttgttca gctcgcggat aaccaaacgg tcgcctttca tacggactcc
     4621 ttttattccg aatacggatt acagttggct ttggtagtag ttgccttcgc caatcagctc
     4681 gatcaggcgc agctgctgtt ccagccagtg ggcgtggtct tcttcggtat ctttcagttg
     4741 ggcgaccatc aaatcgcggc tgacatagtc ttgcgcctct tcgcacagtt tgatgccttt
     4801 tttcaaagcg tcgcgcactt cgtattcggt ttgcaggtcg gctttgaggc aggaaaccac
     4861 gtccgtgccg atattcagct cggcgcgcgc cattttcggc gtaccgccca gcatcaggat
     4921 gcgtcggatg aagtcttcgg cgtgtgtggt ttcttcttcc atctcgtgat tgagacgttc
     4981 aaaaagtttg gtgtagcccc attcggagta gaggcgggag tggataaagt attggtcgcg
     5041 tgccgccagc tcgccagaca gcaattcgtt catataatca acaacagctt gattgccttg
     5101 cataatatct ctctttccgt aacttggttt tcggtatgtc tgaacagcat ttgtgtcgct
     5161 attcgggatg cgtgcattgt atgcaaaagc tgtcggcatg acaaattttc tatttaaaat
     5221 acaaacaatt atcaaaagaa atagggcgta ttccccgatg ccttccaaat cagtatgctg
     5281 tttcttatcg gttttttgtg ctgttaaaac taagaatcca tatcatccat aaagaacatt
     5341 tacacttgtt aaccatagcg aaaagcaaat actgtacggt tttttatcaa aatttataat
     5401 gaatcgttct cattaaccga cagattcttt tgagattatc ggattttggg aataaattat
     5461 gccgtctgag gggctttcag acggcataga caattaaagc tgattaaaca acgcggcaga
     5521 cagcagcgta ggaaattatt ttttacttcg gaacggtatt ttgggtttaa tgatgcccgc
     5581 aaccgccgtg gctttcgcgt aaggcttcgg cagcctgttc gtcggcgtgg tagctggaac
     5641 gtaccatcgc gccgatggcg gcattgctga agcccagttc gtatgcttct ttttcaaaga
     5701 ttttgaactg ctcgggcgta acgtagcgca ggacgggcag gtgtccgtct gaaggctgga
     5761 ggtactgtcc gatggtaatc atttcgatat tgtgcgcccg catatcgcgc ataatttcac
     5821 gcacgtcttc gtctgtttcg cccaagccga ccatgatgcc ggatttggtc gggatatgcg
     5881 gcatcatttc tttataacgt tttaataagt ctaaagaatg ttgataatta gcaccgggac
     5941 gggcttttct gtacaggctc ggatgggttt ctaggttgtg gttcatcacg tcgggcgggg
     6001 tttcggcaag gattttgagt gcgatgtcca agcgtcctcg gaagtcgggg acgaggattt
     6061 cgattttggt gttcgggctg gtttcgcgga tggctttgat gcagtcggcg aaatgctgtg
     6121 cgccgccgtc gcgcaggtcg tcgcggtcga cggaggtgat gacgacgtaa cgcaggttca
     6181 tggctttgac ggattcggcg aggtttctcg gttcgtcggg gtcgagcata ttgggccgac
     6241 cgtgtcccac gtcgcagaac gggcagcggc gggtgcagat gtcgcccata atcatgaagg
     6301 tcgccgtgcc tttgctgaag cattcgccga tgttggggca ggaggcttcc tcgcaaacgg
     6361 tgtgcatctt ttgttcgcgc aaaatgtctt tgatttcaaa gaatttgcgc gatgggagtt
     6421 tggcgcgtat ccattcgggc tttttcagtt tttcctgaag ggggacgact ttgatgggga
     6481 tgcgcgcggt tttgtccgcg cctctgagtt tgatgccgcg tttggggtcg tcggttttga
     6541 tttcactcat tgttgtctgc tttcggtgtg aattgtgttt caaggtgtgc ggtaagtttg
     6601 gcggcgactt cgtccggcgt ggggcagggt tggacaaaat ccgcgatttg cgtcatttcc
     6661 ataccggcgt agccgcaggg gttgatgtgg gtaaacgggc ttaaatccat attgacgttg
     6721 agcgcaagcc cgtgatagac ggagccgttt ttgatacgca gccccagtga ggcgattttg
     6781 cgttctccga cataaacgcc ggggcgtttg gggtctgccg ccgcttcgat gccgtattct
     6841 gccaatgtgg cgatgatgct gttttcaagc gcggaaacga tgtttctaac actggttttg
     6901 cgccgtttga aatcaatcat cgtataaacg accaattgcc cgggcccgtg ataggtaatc
     6961 tgcccgcccc ggtcgatttg gacgacggga atgtcgtcgc gaatcagcag gtgttcgggt
     7021 tttcccgcca gtccttgtgt gaacacgggc gggtgttcga cgacccacag ttcgtcttcg
     7081 gtgtcggcat tccgtccggc attaaaggtt ttcatcgctt caaaagtcgg cagatattcg
     7141 accaaacctt tgtgtatgac tttcatctca aagtaccact ttgaccagtt cgtgcgaagt
     7201 cagcgcgcgg tagatgttgt ccaattgttc ttggttttca acctttatct gaacggtggc
     7261 gccggtatag ttgcctttgc tgctcggacg cgtggtgatg tggtgcgcct gcgtgtcggg
     7321 ggcgtggagg cggacggtgt ctaaaaccgc ctgctcgaac tcgggatgca ccgcgcccat
     7381 tacttttaat gagaaggtgc agggaaattc gatgagggat gttttgtttt tttgttcggt
     7441 catgatgtgc tgccttatcg tgtacggtat gccgtctgaa ggcgggtttg tctttcagac
     7501 ggcatcggat gtgcgttatt ttagcctaaa ccgcgataac aggctatcgg gaaggcggag
     7561 gcttttttga cggcgcggcg gttctgctat actggcgcgc aatattattt ttagaagggt
     7621 ggtttttatg tatcggagga aagggcgggg catcaagccg tggatgggtg ccggtgcggc
     7681 gtttgccgcc ttggtttggc tggctttcgc acttggcgac acactggctc cgtttgcggt
     7741 tgcggcagtg ttggcgtatg tgttggatcc tttggtcgaa tggttgcaga aaaagggttt
     7801 gaaccgtgca tccgcttcga tgtctgtgat ggtgttttcc ttgattttgt tgttggcatt
     7861 attgttgatt attgtcccta tgctggtcgg gcagttcaac aatttggcat cgcgcctgcc
     7921 ccaattaatc ggttttatgc agaacacgct gctgccgtgg ttgaaaaata caatcggcgg
     7981 atatgtggaa atcgatcagg catctattat tgcgtggctt caggcgcata cgggtgagct
     8041 gagcaacacg cttaaggcgt ggtttcccgt tttgatgagg cagggcagca atattgtcag
     8101 cagtattggc aacctgctgc tgcttccctt gctgctttac tatttcctgc tggattggca
     8161 gcggtggtcg tgcggcattg ccaaactggt tccgaggcgt tttgccggtg cttatacgcg
     8221 cattacaggc aatttgaacg aggtattggg cgaatttttg cgcgggcagc ttctggtgat
     8281 gctgattatg ggtttggttt acggcttggg gttggtgctg gtcgggctgg attcggggtt
     8341 tgcaatcggt atggttgccg gtattttggt ttttgttccc tatttgggcg cgtttacagg
     8401 actgctgctg gcaaccgtcg ccgccttgct ccagttcggt tcgtggaacg gcatcctatc
     8461 ggtttgggcg gtttttgccg taggacagtt tctcgaaagt tttttcatta cgccgaaaat
     8521 cgtgggagac cgtatcgggc tgtcgccgtt ttgggttatc ttttccctga tggcgttcgg
     8581 acagctgatg ggtttcgtcg gaatgttggc aggattgcct ttggccgccg taaccttggt
     8641 cttgcttcgc gagggcgtgc agaaatattt tgccggcagt ttttaccggg gcaggtaggc
     8701 ggttccgaaa catatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
     8761 tacaaatagg gcaacgccgt actggttttt gttaatccac tatatttgaa gcggaataca
     8821 accttgcccg ggtttaaata aaaatgccgt ctgaaccccg aaaactggac ttcagacggc
     8881 attttcatca cggcttattt ggcggttttg ctgctgtcga taattttcat accggcagaa
     8941 atcaggctgc cgatgtcggc aacattggcg ggcataatca gcgtattgct ttctttggca
     9001 agattgttga acgcggcgac gtattgttcc gcaatcttca ggttgaccgc atccgcaccg
     9061 ccttgggttt gaagggcggc ggcaatttga cggatggctt cggcattggc ttcggcaaca
     9121 aggcgcaagg attccgcttc acctttggcg cggttgatgc gggcgatttt ctcggcattt
     9181 gacgcattga ccgcagcctg agcctcgcct tcggattgtt ggatttcggc ttcgcgctga
     9241 ccgctggcaa ggttgatttg ttcgatttta cggccttcgg attcggcgat acgggcgcgt
     9301 ttttcgcgtt cggcggtaat ttgcgcctgc attgagcgaa ggatttcttg aggcggcacc
     9361 aagtctttaa tctcataacg caaaaccttc acaccccaag ccccggccgc ctcgtccaaa
     9421 gccgcaacaa cagtactgtt gatttcgtcg cgttcttcaa acgttttgtc caactccata
     9481 cgcccgataa cggaacgcag cgtcgtttgg gcaagctggg taattgccat gatgtagttg
     9541 ctcgaaccgt atgaggcgag tttggggtcg gttacttgga aatagatgat gccgtcaaca
     9601 gtcagctgcg tattgtcgcg cgtgatgcag acctggctgg gtacgtctaa agggatttct
     9661 ttcagcgaat ggcggtaggc aacgcggtcg ataaagggaa tcaaaatatt caaaccggcc
     9721 gtcagggcgc gatggaaacg cccgagcctt tcgacaacgt ggacttcctg ttgtgggatg
     9781 acaacaaagg atttgaaacc gaaaacggca acggctgcca acaagataat gaaaaattcc
     9841 ataattcctc cgagtgttaa ggttttgcga tgataagaag gttgccttcc ttgcggacga
     9901 ttagggcgcg cgttcctggt tcaagctctt cttgccccgt attttgcgcc tgccagtgcg
     9961 taccgcgata aaaaacttcg taacggttgc cgcctgtgtg tcggaggatt tcgacatatt
    10021 gtccggcatc caaatcctga tatgaatccg tttcaacttt tctaacggcg gttttggcgt
    10081 gtacgaacca aatacccagc gcggaaagca gagcggcggt caagacggcg gcaggcgtgc
    10141 tgccggtcag cccgtaagca atgcccgaac ccgccaaagc cgcgctgaca accaaaagat
    10201 aaaccgttcc cgtcaataat tcgatgatta agacggcaac agcggcaaca aaccatacag
    10261 tcatacattt ccccacaaag cgcgtcgttt gacaaaataa cgcaatatca gcagtatagc
    10321 cgaatttgaa aggatagggc agatatggac acttggcacg atgcactcgg cggcgaaaaa
    10381 cagcagccgt attttcagga aattttaaat gcagtcaggc aggaacgttt gtcgggacaa
    10441 atcatctatc cgccggcggc ggatgtgttc aacgcattcc gcctgacagc gttcgaccgg
    10501 gtcaaagccg tcattctcgg acaagatccg tatcacgggg cagggcaggc gcacggtttg
    10561 gcattttccg tccggcaggg tatccgcata ccgccgtctt tactcaatat ctacaaagaa
    10621 ttggaaaccg acatcgaagg cttttccata cccgcgcacg gctgcctgac agcgtgggcg
    10681 gagcagggcg tattacttct gaacacggtt ttgacggtgc gtgcaggaca ggcgcattcg
    10741 cacgcccttt taggctggga acgctttacc gataccgtca tcaggcagct tgcgacacac
    10801 cgcaagcatc ttgtctttat gctgtggggt gggtatgcac aacaaaaaag gaaactgata
    10861 gacagtcaaa accatttgat attgaccgca ccgcatccgt ctcctctgtc ggcatatcgc
    10921 ggttttttcg gctgccgcca tttttcacag gcaaacagct atttgagccg gcacggtatc
    10981 gatccgataa actggaagct gtgaatgcca tctgaatgcc aagttgctta aaccccactg
    11041 aaggctgctt ttgaaaccgg aaacaaagtg attaaggaaa accggcccat aatgcaaaac
    11101 cgtctgaatc ctagggttca gacggcattt tttgccgcca tttccaaaaa caacttttgt
    11161 tcgcctgccg acaaatccga caaagtcgtt tggttgaggg ctttggcaat aatttaacat
    11221 aatatacatt atgcgaacta tcggaaacag ttgtacgtgt ccctgtggtc tttccaagta
    11281 ggaaaattaa agtatggaca atgcggctga atgtatagcc cggagcatcc gcgtatccga
    11341 aggtactgaa cgatatgccc tgcatccctt tcgatgcttc catccagccg gtattgggaa
    11401 atacgttgac cttcaatata atatacaggc agccgacaca caggatatgc cgctgctttt
    11461 tcatcatttc ttctgtcaaa tccttggaac ggtcgatttg aaaacacggc tttacatacg
    11521 tcccaattct ctttgcagtg cttggatatt ttgctgcctg tccaatacat tgctttgtaa
    11581 tgcatttatt tcttgatggt tgatgttgcc gccctttgcc agacgtgctt gtgataacat
    11641 tttttgggct tcagtcaggg ctttgcgttc gttgctcaat tctgcttcga gaatggagcg
    11701 tctgctgttg tttacaggtg cttgttgagg cgggggcgta ttggattttg ccggcttgga
    11761 tactgttttg accggggctt tatatttgac agcctgtccg ccgtttgacg gtgatggtgt
    11821 cggttcggat gtttggggcg ggatatagcg ttcgctgctg tagttgccga ttgggggcaa
    11881 atcggttgag aggcagcttt tggacggctt ggtggtgtaa acggtttctc cgttgattgt
    11941 gcaggtgtag attttggccg cattcgcacc caatgggctt gaaatcaggg aaaagttgat
    12001 taggattaag gggagttttg atttcataat gtgcttgatg ctcggataat tatttgattt
    12061 tttggtattt tttgtaatgc tttcttggcg ttttacgggc ttttcttttc ggttgtttgc
    12121 ttttgatttt ctttcggggc tttggcagcc tgcttgttct ttttcccggc aggttttttg
    12181 tttttctttt tactttctat gtgtattttg gcttcttaac tgagtttttt aattttcagg
    12241 cggtatccgc ctccctgatg gctgctgatt ttaggtaaat ccgcatcggc gcacgatcct
    12301 gctggggccg atgtatatac gcttttgcca tttgagttgc aatggtatac ggaggcttgc
    12361 gccgccgcgc cggaaaggga caggagaccc aaggcggcaa aaagtctgat gtttataccg
    12421 gtaaaaggcg gtgataacgc ccgaattata ccgttattgg caggcaaaga taagcaccct
    12481 gtccgcgctt ctttatcgct cggcaaactg tttctcgatt tcagtttcaa tccgttccaa
    12541 ttcttcttga caagccagcc aaacctcttc gatttgggca agttgtgttt tgacttttgc
    12601 cagcagggat aaggtatttt gcaatttttc tttgtttgct tcaaggtaag cgtcttcttg
    12661 tgctaaaaat gcttcacatg ccgtctgaat ttcggaaagc tgcgccattt ctttttcggc
    12721 acggtctatt ttctgctgta tcggcttgcc gcgtcgggct ttttcctgac ggatttgcgc
    12781 ttcgatgcgc ttggtgtctt tgcggctttg gctttgtgcg gatgctgcgg gtgcggcggc
    12841 ggcgttttcc tgtgccaaac gccattggcg gtaatcgttc aaatcgccgt cgaagttctt
    12901 cagacggcct ttgtcgatca ggaggaagct gtcggtagtc gcttcaagca ggctgcggtc
    12961 gtgcgatacg gcgattaatg cgccttggaa actttgcaac gcgagcgtta aggcgtggcg
    13021 catatccaag tccaaatggt tggtcggttc gtcaagcagc aacaggttcg gcttttgcca
    13081 gataatcatg gcaagggcaa ggcgggcttt ttctccgccg gaaaaaggtt cggttttctg
    13141 taacgccata tcgccaacaa aattgaagcc gccgaggaaa tttcggattt cttgttcgcg
    13201 tacttcggga gaaagctgct gaatatgcca aacagggttt tggtcggcgc ggatggtgtc
    13261 gagttggtgt tgggcaaaat agccgatatt gagtttttcg gaacggatga tgctgccgga
    13321 aagcaaatcg attttgcctg ccaaggcttt gataaaggtg gatttgccgc tgccgttgac
    13381 acccaatagc ccgtagcgtg cgccgctttc cagcgacagg gtaatgtcgt gtaggacagt
    13441 tttgccttcg taacccaaat cagcgtgttc tagctttaac aaaggattgg gcagatggtc
    13501 gggatggtaa aactcaaagg aaaactcgct gtccagatgc gcgggcgcaa cgcgttctag
    13561 ctttgccaaa gccttcatgc ggctttgcgc ttgaacggct ttggtggctt tggctttgaa
    13621 gcggtcgata aaggattgca aatgtttgat ttgcgcctgc tgtttgacat aggcagcttg
    13681 ttgttgcgcg agacgctgcg cgcgttcgtt ttggtaaaaa tcgtaattgc cgccgtattg
    13741 cgtgaatttt tgctgcgata attcaatggt ttgggtagtt gccgcgttga gaaaatcgcg
    13801 gtcgtgggaa atgatgattt gcgtgcaggg taaagaagca agatggtttt ccagccacaa
    13861 gacggtttcc aaatccaagt ggttggtcgg ttcgtccaaa agcagcaaat cggcgcggca
    13921 aatcagggct tgggcgagat tgaggcgcat acgccagccg ccggaaaagg atttgacggg
    13981 gcggctgtgt tcttcttgcg aaaaacccag cccgttcaac aattttgccg cgcgggccgg
    14041 cgcggtataa gcgtcgattt cttccaattt ggcatgatat tccgcctgct tcatgccgtc
    14101 attttgcgct tctgcctgcc tcaatgccgt ctgaaaagcc tgcaactcgg catcgccctg
    14161 caaaacgtaa tccaaagcgg aaatatccaa atcgggcgtt tcttgggaaa cggaagcgag
    14221 ccgccagttt ttcggaatcg agacatcgcc gccgtcctga gtgatttcac ccttgattaa
    14281 ggcaaacagg ctcgatttgc ccgttccgtt tttgccgatc aaaccgacgc gctgaccggg
    14341 attgacggta gcgttggctt tgtcgagcag gactttcaaa ccgcgttgca gggtgaggtt
    14401 tttgatttca atcataacgg aaacatcgtc gggcgggaaa agcccgtatt ttacctgaaa
    14461 gtcagtgccg atgccgtctg aaacgggaaa tttacggctg aagccaagcc caagccctgc
    14521 gcccttccga gtgcaggaaa accaatgtcc tgaatgccga atcggtattc atgcattcca
    14581 cgctgattcc gattcgggaa aaatccgcca tgattttggg atggataaac tcctgagccg
    14641 cgcccgtccc gataatcaat atttccggat agtcaacagg tttgatgtcg gacaacaggt
    14701 tttccggagt cagatcggac aaggttcggc attgcgacag gcagaccgaa tccttatgta
    14761 caagcacggg tttatggaaa ctttgccccg ccagccggat tccgcccgca ccgcattcat
    14821 attccgcaaa ctgtccgtct atcggatttt cttcaaacaa cattttttta ccccgtcgcc
    14881 gcatcatcta caccgaaagg ggtgcaaaat cagacaaatt catgtaggat tggcagattt
    14941 catctgactc gcctgccgat ttcagacggc atttgattca aagtgcggca ccattatatc
    15001 ggcagcggat attttcgtct ttcaatattt acatttcagt cggcttacaa ggagacacaa
    15061 tgaagccagt aaacatcggt cttttaggtt tgggtacggt cggcggcggt acggctgccg
    15121 tgttgcggga caacgcggag gaaatttccc gtcgcttggg gcgcgaaatc cgtatttctg
    15181 ccgtatgcga cttgagcgaa gaaaaagccc gccaaatctg cccgtccgca gcctttgtca
    15241 aagatccgtt cgaactggtc gcgcgtaaag acgtcgatgt cgtcgtcgaa ttgttcggcg
    15301 gcacgggcat tgccaaagat gcggtgttga aagccattga aaacggcaaa cacatcgtta
    15361 ccgccaacaa aaaactgctc gccgaatacg gcaacgaaat cttcccgctg gcggaagaaa
    15421 aaaatgtcat ggtgcaattt gaagcggcag tagcgggcgg tatcccaatc atcaaagccc
    15481 tgcgcgaagg tttggcggca aacaggatta aatccatcgc cggcattatt aacggcacca
    15541 gcaacttcat cctctccgaa atgcgtgaaa aaggcagcgc gtttgccgat gtactgaaag
    15601 aagcgcaggc actgggctat gccgaagccg acccgacctt cgacatcgaa ggacatgacg
    15661 cgggccataa aatcaccatc atgagcgcgc tggcattcgg cacgccgatg aacttttccg
    15721 cctgctacct cgaaggcatc agcaaactcg acagccgcga catcaaatac gccgaagaac
    15781 ttggctaccg catcaaactg ttgggcatca cccgcaaaac cggcaaaggc atcgagctgc
    15841 gcgtccaccc taccctgatt cccgaaagcc gcctcttggc aaacgtcaac ggcgtgatga
    15901 acgccgtgcg cgtcaacgcc gatatggttg gcgaaacctt atattacggc gcgggcgcgg
    15961 gcgcattgcc gaccgcttcc gccgtggttg ccgatatcat cgacatcgcc cgcctggttg
    16021 aagccgatac cgcccaccgc gtaccgcatc tggcgttcca acccgcgcaa gtccaagcgc
    16081 aaaccatcct gcctatggac gaaattacca gcagctacta cctgcgcgtc caagccaaag
    16141 acgaaccggg cacgctgggg caaatcgccg cgctgttggc acaagaaaac gtgtccatcg
    16201 aagcactgat tcaaaaaggc gtgattgatc agaccactgc cgaaatcgtg attctgaccc
    16261 acagcacggt cgaaaaacac atcaagtcgg caatcgcagc catcgaagca ctggattgcg
    16321 tggaaaaacc gattaccatg atccgcatgg aaagcctgca tgactgagcc gaaacacgaa
    16381 atgccgacgg aagagcaggt tgccgcgcgc aaaaaagcaa aagccaaaat ccgcaccatc
    16441 cgcatttggg catgggtcat tttggcgttg ctcgcttcaa ccgccctgct ctcccaatgc
    16501 gcgatgtcca aaccgcaggc aaaacagaaa attgtcgagt cttgcgtgaa gaatattccg
    16561 tttgccgaaa aatggcaaaa cgatttacgg gcccgcggtt tagattcaaa caatacccgc
    16621 cttaccgtcg actactgcaa atgtatgtgg gagcagcctt tggacagatt gagcgagaaa
    16681 cagattagat ccttcggcaa actcggcgca caagaacagc ttgacctgct cggcggcgca
    16741 aatgcctttg aagcacgtga caagcagtgt gttgccgatt tgaaatcaga ataatgtgga
    16801 ccgataaaaa agccgattct ttaaagaatc ggcttttttc ataaaaaacg gcttacagtg
    16861 cgtctttcaa agctttgccg gcgcggaatt taggcgtttt ggcggcggca atggtcagag
    16921 gctcgccggt tttggggttg cggccttggc gttccgcacg ttcgcccacg tagaaagtac
    16981 cgaaaccgac caaagtaacg gtgtcgcctt gtttcagggc ggtggttact gcattggtag
    17041 tggcatccaa agctttttgt gcggcggctt tggaaatgtc ggcttcttga gcaatcgctt
    17101 cgatcaattc agacttgttc acaatcagtc ccttcctgtc ttaaaaaatg atgaaatgcc
    17161 cgaatactcg gggtttgtac tgcttgagca actttcgctt tatagcaatt ctgaaattgc
    17221 cgtgtcaagc aaaaaatacg gaatcaccct atttgacagg ctttcaggac gaaaccgcat
    17281 ttttacaaca catttcctgc gtttcaatgt ttggttgccc tgctgcgggg ttttggtttt
    17341 gaagcggatt ccgccgccgc ttccgcacca gaaggttctg cccaaggctc aggctggctt
    17401 tccaaaccca gagccaatac ctcgtctatc catttgaccg gatggatggt caggccggtt
    17461 ttcacgtttt cagggatttc ttccaagtct ttgaggttgt cttttggaat taaaacgtgt
    17521 ttgatgccgc cgcgaagcgc agccaacagt ttttccttca gaccgccaat cggcaggact
    17581 tcgccgcgca gggtaatttc gcccgtcatg gcaacatcgg cgcgcaccgg gattttggtg
    17641 aatgcagaca ccatcgccaa agtcatggca atgcccgcac tcggaccgtc tttcggcgtc
    17701 gcaccttcgg gaacatggac atggatgtct tttttctcgt aaaaatcagg agccaagccc
    17761 actgattccg cgcgggagcg gacaaccgac cacgctgcag acacggattc cttcatcaca
    17821 tcgcccaact ggccggtgca ctgaatcacg cccttacccg gcaatgctgc ggcttcgacg
    17881 gtcagcagtt cgccgccgac ttctgtccac gccagaccgg ttacttgtcc gatacggttt
    17941 tcgctttcgg caacgccgta atcgaagcgg cgcacaccca aatagtcgtg cagatttttc
    18001 tcatttactt taaccgcttt aggtttggct ttgctggttt tcttggtttc agacaacctc
    18061 ttcttatctt cgtccaaggt aatctgcatc accaccttgc ggcagatttt ggcaatctcg
    18121 cggtcgagcg aacgcacgcc cgcctcgcgg gtgtagtaac ggataatatc gcgcaccgcg
    18181 ctttcttcga ttgccaattc cccttctttc acgccgttgc gcttcatttg cttcggcacg
    18241 aggtactgca tcgcgatatt gattttttcg tcttcggtgt agccggacag acggatgatt
    18301 tccatacggt cgagcagcgg ggtcgggata ttcaggctgt ttgaagtcgc aataaacatc
    18361 acatcgctca aatcgtaatc cacttcggca taatgatcgg cgaacttgtt gttttgctcg
    18421 ggatcaagca cttcgagcaa cgcgctggcg ggatcgcccc ggaagtcgct acccaatttg
    18481 tcgatttcgt cgagcaggaa caatgggttt tttacgccgg cttttgccat attctgcaga
    18541 atcttaccag gcatggagcc gatataggtg cggcggtgtc cccggatttc gctttcgtcg
    18601 cgcacgccgc ccaaagccat gcggacatat ttccgccccg ttgctttggc gatggattcg
    18661 cccaaagagg ttttgcccac gcccggaggg ccgaccaggc acagaatcgg gcctttgagt
    18721 ttgtccatac gtttttggac ggcgaggtat tccaaaatcc gttctttgac tttttccagg
    18781 ccgtagtggt cggcatccag caccagtccg gctttggcga tgtctttgct gacgcgggat
    18841 tttttcttcc acggcagttc gagcagggtg tcgatgtagt tgcgtacgac ggtggattcg
    18901 gcagacatcg gcggcatcat tttgagtttt ttcagttcgg acaggcattt ttcttccgct
    18961 tctttgctca tccccgcctt tttgatgcct gcttccaagg catccagttc gccgttttcg
    19021 tcttcttcgc ccaattcttt gtgtatcgct ttaatctgtt cgttcagata atattcgcgc
    19081 tgggattttt ccatttggcg tttgacgcgt ccgcgtatgc gtttttcggc ctgcataatg
    19141 tcgagttcgg attccagctg tgccagcagg aattccatcc gtttgccgat ttcgggaatt
    19201 tccaaaatct gttggcgttg cgccagtttc aactgcaaat gcgctgcgac cgtatcggtt
    19261 agccggctgt tttcggcaat gccgttaatg ctgccgataa tttcggcggg gattttttta
    19321 ttgagtttgg cgtattgttc aaactgcgcc aacagggtgc ggcgcacggc ttcgaggtcg
    19381 gtattgccgc ccgtgtcttc ttccacgacc gtctctatat gggaaacgaa cagaccgccc
    19441 gtgtcttcaa tggtcagaac acgtccgcga tacagccctt cgaccaatac ttttaccgtg
    19501 ccgtcgggta gtttcaacac ttgcaggact tgtgcgaccg taccggtctg atacaggtcg
    19561 gcggcaaccg gttcttctac cgccgcatcg gtttgcgcca acaggaaaac cggctcctcg
    19621 cgggtaatgg cgttttccag tgcggcgatg gatttcggcc tgccgacaaa cagcggcaga
    19681 accatatgcg ggtaaacgac gacatcccgc aaaggaaggg ttgccaaggc ggcatattcc
    19741 tcaaaatgct tttctttttg tgtcataggt actctcttgt gtctgacaga ttgccgattt
    19801 tcgcgtacat tggggttgaa ggtattattt caagcatatg tggtttattt atggagtttg
    19861 atgcgatgcc gtctgaaaca ttccggcttc agacggcatg ggcttggaaa gacaaggcgg
    19921 gaacaaaaaa ctgttctgtg ttgccgctcc ttgctgtacc atccgtatgg tttgcggttc
    19981 tgccgcccta ttttcaaaac atgacgcagg tattccatgt cttcttttat ttttcccgat
    20041 aacgggttcg aatggcgcaa cgaaagtctg caaaagccgc ccaaaggttg gcattatgtc
    20101 cgcgaaagtg gtgcaaacgg cattttgaag gctgcctatc aaaatattgc aactgtctgg
    20161 cagggcgatt tccacaatgc caaacaggtg ctttccgcaa tgaagaagaa ggtccgcaaa
    20221 cctgccgccg tccgttccga tgcggatatc gccgcccttt tccacgccca ccgtatgaag
    20281 caggcgcagc agagccgtat tctgaatatg cttgccgttg aaatccgccc cggttttgtg
    20341 ttggacaaca aacgcgcgcc cgatatacgc gccgccctgc tcgacgtgta cggagaggcg
    20401 gacggcaaac cgtttttcct gccgctcaat ctgctgctgg ggtttatggg tgcgcacgag
    20461 tggcataaga aaggggttgc cgttccgcag ctgggcggca gcatacacgt tcctttcggc
    20521 gtattctcgc cgttgcgcgg cgaatacctc gacctgctcg cccatgcgcc gtcaacgggt
    20581 tttcagacgg cattcgatat cgggacgggc tcgggcgtgc ttgccgccat tttggcgaaa
    20641 cagggcattc cttccgtcat cggcacggat accaatccga aagccgtcgc ctgcgcccgt
    20701 gccaatattg cccgtttggg ctttgaaaaa caggttgaga tacgggaaac cgatctgttt
    20761 cccgaagggt ttgccgatct gattgtctgc aatccaccct ggcttcccgc caagccgact
    20821 tccgccgtcg aatccgcgtt atacgacccc gaatctgcga tgctggctgc gtttttgcgg
    20881 gatgcgccga aacatctgaa tcccgacgga gaaatccgcc tgatcatttc cgatcttgcc
    20941 gaacatctgc acctgcgtcc gtccgatttt ctggataagg catttgctca ggcgggtttg
    21001 cgcgttgcgg atgttttgaa aaccaaaccg gttcacaaaa aagctgccga tccgaacaat
    21061 ccgctggctt ttgcccgaaa cagggaaact actttcctgt accgtttgaa aaaggcataa
    21121 ggggcggcgg tgcgcttccg gcggcaggca tcggaacgcc cgcgccgccg gcacggcatc
    21181 attcggccgg attatccttg tgaaaatacc cgctcaagca tactgcccaa tgccgtctga
    21241 cgcgttttga cggtggcggc agcttgccgg aaggcttctt ccccgccgaa gaatacgaac
    21301 ttctctctcg cgcgggtaat ggcggtatat aacagctcct tactcaatcc ggacaatgca
    21361 tcgtcccctt cgtccgaacc gccggcggag ggcggcagca gccaaacttc ccgatattcc
    21421 gaaccctggc ttttgtggac ggtcagcgcg aaagcgggtt cgaaatcggg caggcagctt
    21481 accgcaacct ttttaaatcc gtccgtatcg gggaaatagg cggcaaggct gccctgtcgt
    21541 ccgacatctt ccataatcag tccgatgtcg ccgttgaaca gttcaagcgc gtagtcgttc
    21601 tgcctgatca taatcggctc tccggcaaaa tatgccaaat gttccggtat gttcattttg
    21661 cggcgtacat ggcggcaata ggcttcgttg aagtcttccg catcctgccg ccaagctgcc
    21721 agaaccacga tatccgaaat gcccgcatat gcggcttcga tattgccgtc ttttaccgcc
    21781 tgccaatagg ctttgtgcac ccggtacaac ctttcgactc gagcgttcgg actgcattcc
    21841 gaatgttcca gttcgtccgg aaaccggtca aacaatgccc acgccccctc atcgcccgat
    21901 acggcggtac gggcaaggca gccgatgccg ctgttgtcgc cgaagcggtg gctgaacgac
    21961 agatgggcgg tgttttgcgc caacacgggc ggatttgcgc tgacgctgaa accgtgttcc
    22021 ggaaggaagc cggccagcct ttggtgcgtt tctccgtcca aaacggtttt ttgcgacaaa
    22081 acggacagca ccgccccgat tccgacggac gggagctggt tttcatcccc cagcagaatc
    22141 acgcgcgcgc cggttttgac cgcttttaaa agttgcagca tcaatgccgt atccagcata
    22201 gaggcttcat cgataaccaa tacgtcaaac ggcagcgggt aaacagggtt gaacgccgcc
    22261 tgcattttgg gcgggcgcag cttcagcagt cggtggacgg tttgcccttc cagtttgagc
    22321 aaatggcggc ggacggcctc cggcgcgtca aaaccgttga ttgcacggtg cagcgcgcgc
    22381 gccatatgtg ccgccgcttt gcccgtcggt gcggcaagcg cgatgttggg aagattttcg
    22441 tcttcaccgc aaatcagcgc cagcagtttg gcaaccgttg tcgttttgcc cgttcccggc
    22501 ccgccggtaa tcaccataaa agactgcaac agtgccaagg cggcggcatc gcgctgccct
    22561 tcgctgcccg tgccttgaaa ccattttgcg aggttttgcc tcgcgcctgc cgcgtcgggg
    22621 gcggacgtgt cggctgccgc caagcgtttt atctcggcag ccaaatcgta ttccaactgc
    22681 cacatcctgc ccaaaaacag ccttctgcct tccaaaatca aaggcgcggc ggatgttccg
    22741 accacgggtg cgagtgccga cagcgcgtca gcctcgccac cgctcaaacg gataaacgaa
    22801 tgaccgtttt gcaatgcctg aaacaggcgt tcggtgcagt ttgcaagcac ttcgctgccc
    22861 gaacccgcat agcgttccaa aaaccggatt gccgcccttg ccgccgcttg ggcaaattca
    22921 tctgtctgcc gttccattgt attccttatc caaatgccgt ctgaaggcgt ggggcttcag
    22981 acggcgcggt gttgtttcgg ctgtttaggc gtttgcgccc tgtttggggt gcaggctgcc
    23041 gtctttcatg accatcacgc gctcgaagcg gccggcgagt tcgtcgtcgt gcgttacgac
    23101 caccagcccc gttcccaatt ctgttttcag ttccagcatc atatccaaaa cattcctggc
    23161 gttcgcacga tcgagattgc cggtcggttc gtcggcaagc aggcatttgg gttgggtaac
    23221 caaggcgcgt gcaatggcgg cacgctgccg ctcaccgcct gaaagttcgc ccgcgcggtg
    23281 cgtcgaacgg tgtttcagtc cgaccttttc cagcatcgcc attgccgcct ccgcagcctc
    23341 ttcacggctt tttttgccga tcagaagcgg catcattacg ttttccagtg ccgaaaattc
    23401 aggcagaaga tggtggaact ggtacacgaa accgagatgg tggttgcgca ggatgcccag
    23461 ttgtcgctgg cttaatgtac gtaaatcctc gcccatcagc agcaccctgc cttcagacgg
    23521 catatccagc ccgcccaaaa tatgcagcag cgtcgatttg ccgctgcccg aagaaccgat
    23581 gatgccggtg ctttcccccg cgcggatttc caaatccaag ccgtgcagca ccagaacgtc
    23641 caaaccgccg tcccgatagc gtttgcccac gccttcgcat ttcaaaatca actcactcat
    23701 aacgcaaagc ctccgccggt tgggtttttg acgcgcgccg gctcgggtag agcgtcgcca
    23761 cgaaagacag ccccaaggaa atgcaggcaa tcagggcaac gtcgcccata tcgacatcgc
    23821 tgggcaggta gtcgataaaa taaacctgcg aattgatgag gtggacaccg agcaggtttt
    23881 caaaaaacgc cacgaccctg ccgacgttcc aacccaaaag cacgccgcag accacacccg
    23941 ccaacgtgcc gaaaaagcct gaaaacgcgc cctgcaccat aaaaatcttc atcacgccgc
    24001 caggggaaag acccaaagtc cgcaaaatcg caatgtccgc ctgcttttcc gtaaccgcca
    24061 tcaccaagga agagacaagg ttgaacgccg ccacggcgat aatcagcgtc aggatgatga
    24121 acatcatccg tttttccaac tcgaccgcct caaaatagct tcgattgctg aacgtccaat
    24181 cgcgcaccca aaccgtatcc ctttgcgcct ccggaatcag tgttgccgtc aaggcggggg
    24241 cgttttgcgg gtcggcgagt ttcagccgca gccccgcaac ttccttatcc aagcggtaca
    24301 gtacgcgcgc gtcttggata tgcgtcatcg ccaatgagtt gtccacttcg taaacgcccg
    24361 tcttaaccag accgaccacg gtaaactgct tcaggcgcgg cacgactccg gcaggggtaa
    24421 cgttgccctc cggcgtgatg acggtaactt tattgccgac ttccgccccc aaagcctccg
    24481 ccaagccgac accgaggata atgtcaaact cgcccggaat caggtcttca aatttgcctg
    24541 ccggcatttt gtcgccgtat tccaccactt tgcgctcttc agacggcaaa atgccgcgca
    24601 tctgcacgcc cctgatttcg cccgcattgg ccagcaatgc ctgattggaa acatagggcg
    24661 cggcagccaa aatacctttg cggttttcgg taaaccgaag caggttgcgc caatccgtat
    24721 ccgtattgtc gatatagccg atttcggcgt gcggcgcgac attcaggagc tgcccgcgta
    24781 tttctttctg aaagccgttc ataaccgaca agacgacaat cagcgcggtt acgcccaagg
    24841 cgattccggc aatcgaaacc atcgtgataa acgacataaa gccgttgcgc tttttcgccc
    24901 tgagatacct caagcctatc caagcctcta gagaaaacat aacgctacct taaaaatgtc
    24961 tgcaaacgtg ccgccccgga cggcggtttg ggggcggcgg caaaagttta ttgtaccgta
    25021 aaaccccggc agcgtccgaa cgcccgagtg cgggggacgg ggcggcagag cgggcggaaa
    25081 acttgcacaa cgcgtcaaac tgccctatcc tttccttcag aaaaaccgtt tcttgaggaa
    25141 aacaatgaat atccgaactg cttttgcttt gtgcgccatc gccttatccg ccgctgcggc
    25201 tgcctacgcc aaagaaatca aaatcgatgc caacaacacg ccttattccg aagccgacgc
    25261 gcaaaagctg gcggcaacgg cagtcggtat gggtgttaag gaacctatca gcctgaacgg
    25321 cggcagcgac agcattaccg tgtccggcag cagcgcgacg cagtgcgtgt tcaaagtcgg
    25381 caacggaggc gcattgcaga ttcaagggct aaactgcaag taaaccgccc ggaaaaatgc
    25441 cgtctgaagg cttcagacgg cattttgcat tggcggcgtt atgccccgcc ttctttaatc
    25501 aggcggcgtt cgtacaccgc ctgcgccagc gttcccgcat cgacatattc caattcgccg
    25561 cccaagggaa tgccctgcga cagcctgctg actttgtaag gcaggttttt aaaaaactcg
    25621 gacaggacat acgccgtcgc attgccttct gcggtaaaag cggttgcaat aatgatttct
    25681 tcgacttccc cgccgcccag ccgttgcgcc agcctgtcca atgcgatggc ggatacgtcc
    25741 attcccaatg ccgtattgat ttgccccatc aggacgaaat acagcccgtc gtggcagttt
    25801 gccgcttcca tattcgacac gtcggcaggc atatgcacca ccatcagccg ccgcccgtcg
    25861 cgtgtttcat cggcacaaat atcgcacaat ccgccttcgc aaaacgtgtt gcacatcgcg
    25921 caatggtaaa cctgcttcaa tgccgtctgc aaggcatcca ccagtttttc agcctctttg
    25981 cgcttgtgtt ggagcaaatg atacgctatc cgctgtgccg atttcggccc gacgttgggt
    26041 aaaaccttca gcgcgtcgat caatccttgg aaggcatctt gttttttgtg gctcatcata
    26101 ttccgccgta tgggaaaacg gccggaatat tccgaccgtt atgttgtcaa caaaagtgtc
    26161 aattactgac cgtcgccgtt gtcgaccgat tgcgctcctt tggtctgttt gattttgccg
    26221 ttgaaataac gtatcaacaa gtcgaaggta ttggcagact gctgctgcgc caaagcctgt
    26281 tttgcaagcg ggatgcgcgc ggcggcatca tccggcgggg ttacagcctg tacttcgaca
    26341 atcacgggtg ccggcaggcc ggtcagcctg acataggcgg gtttgccgtt tgccggtttt
    26401 actttcagca gttccgcata agcctcgggc ggcatggact gccttgcctg ctgtgcgccc
    26461 aaaacggaca cttccgacca tttcacgtca actgccttgc cgccgttcag ttgggtaagc
    26521 acgtcttttg ccttgttttc ggcaagtttg gcggcttcgg tacggatata agcctgacgt
    26581 accgcgtctt tggcttcggc aaacggcagg gttttctctt cgcggacttc tttggcgcgg
    26641 acgacccacg cggtttcgct gttgatggtc agcacttcgg aattgtgttt tttcttcaat
    26701 acgtcgtcgc tgaatacggc attgatcagg ttttcgggca taccggacat ttgcgcatcc
    26761 tgcctgctca gccaagtttc ttgggtttcg actttcaaac cgctgttttt ggcggcttcg
    26821 gcaagcgagg aaggatggtt gaacgcgtca tcgcccagct tttcttttgc cttattgaag
    26881 tcggcaaccg cctttttcat tttcaattcg ttttctacgg cggctttttc ctgctcgaaa
    26941 gaaggtttgg cttcatttgc cggcaaacgc gccacgcgct cttcaaacgc atttttcact
    27001 tctgtttcgc tgacggtctg tttgtctgca aagtctttca gattcaaggc gacatattcc
    27061 aatttgaccg ctttgggaag caggtagtct tttttgtttg cgttataaaa cttctgcaaa
    27121 tcggcttcag acactttgac ttgggcgatg aattcgtcgg ggttgaaagt gtgcgaacgg
    27181 atggtgcggt tgacctgcgt cagcctgatc agctgttccg cctgcgcgtc gccgaccaat
    27241 acgccgtttt ggacgaggtt taccaaattc tgcaaggcaa actgatcgcg gatttcttcg
    27301 acaaactggt cttcagacat atggcgttgc gacaggtagc ggtttaaaag cgcgtggtcg
    27361 aatttgccgt ttgcgtcgtg gaaattggga tcatccacga taatctgctt gatttgttcg
    27421 gaagaaaccg aaatgcccat cagcttcgcg ccctgtttca ggtaggcgcg ttgcagcagg
    27481 gattggaaca ccgcgtcgcg cgaagggccg ccgccgtccg cctgttcgtt ctgtatggcg
    27541 ttgttgatgg agtggtcgct gattttttcg tcgcccactt ggacgatgta gtcggcaccc
    27601 ggatgggata ccgtgctgac cccgaagccg acgaaggtta atgcaatcag gcccaaaagg
    27661 acttgggcgg gcgttctgta tttttcgatg gaatggaaca tattttaaat cgggatatag
    27721 aatgggaacg ggaaattcaa gtcgggtatt gtaacggttt ttatccctgt ctgcacgggg
    27781 cttgccggtt gaagatgccg tcgtaggttt cttcgctgaa gccggcgtaa accttgccgc
    27841 cgcactccaa tacgggacgc ttgatcaggc tcggcatttc ggacatcagt ttgacggcct
    27901 ccgctgtcga gaacagggct ttttgctgtg tttcggcatc gagtttgcgc cagcttgtcc
    27961 cgcgtttgtt gagcagggtt gccaaaggca cttgttccag ccacgagcag atttccgctt
    28021 cggaaggcac ctgtttttta aaatcccgaa attcaaactc caagccgtat ccggcaagcc
    28081 ggtttttggc ttttttgacc gtatcgcaat ttgggatgcc gtgaaggact atcatttgga
    28141 aaccttttgt ctgaaataat aaaacggata ttttactata agtgtctgaa aatttgcccg
    28201 tctgtttcag acggcagggc ggttatgtta caatccgaaa attcgaaaaa tttaatctct
    28261 tgttcaataa aggctttacc aatcatgatt tctaccaacg gcatcaccat gcagttcggc
    28321 gcgaagccgc tgtttgaaaa cgtatccgtt aaattcggcg aaggcaaccg ctacggtttg
    28381 atcggcgcga acggctcagg caaatccacc ttcatgaaaa tcctcggcgg cgatttggaa
    28441 cagacggccg gcgaagtggc gattgaaaac ggcgtgcgtt tgggtaaatt gcgccaagac
    28501 cagtttgcct acgaagacat gcgcgtgctg gacgtggtga tgatggggca taccgaaatg
    28561 tgggcggcga tgaccgaacg tgatgcgatt tacgccaatc ccgaagccac cgaagacgac
    28621 tacatgaaag ccgccgaact ggaagccaag ttcgccgaat acgacggcta caccgccgaa
    28681 gcgcgtgccg ccgaactgtt gagcggcgtg ggcatttccg aagatttgca caatgcgaaa
    28741 atggcggaag tcgccccggg cttcaaactg cgcgtattgc tggcgcaggc gctgttctcc
    28801 aagccggatg tattgctctt ggacgaaccg accaataact tggacattaa taccatccgc
    28861 tggttggaag gcgtgttgaa ccaatacgac tccacgatga ttatcatcag ccacgaccgc
    28921 cactttttga acgaagtctg cactcacatg gcggatttgg actacaacac catcaccatc
    28981 tatccgggca actacgacga ctacatgctc gcctccgccc aatcgcgcga acgcgccctg
    29041 aaagacaatg ccaaagccaa agagaaactg caagagctgc aagagttcgt cgcccgcttc
    29101 tctgccaaca aatccaaagc ccgtcaggca accagccgtc tgaaacaggc cgacaaaatc
    29161 aaatcggaga tggtcgaagt caaaccttcc acccgtcaaa acccgtatat ccgttttgaa
    29221 gccgatgaaa aagccaagct gcaccgtcag gctgtggaag ttgaaaaact ggcgaaacgc
    29281 tttgaaaccc agttgtttaa aaacctgaac ttcatccttg aagcgggaca acgcctcgcc
    29341 atcatcggcc cgaacggcgc gggcaaatcc accctgctga aactcttggc cggcgcgtac
    29401 aaccccgaat attcagacgg cctgttaccg gacgaaggca ccatcaaatg ggcggaaaaa
    29461 gccagtgtcg gctactatcc gcaagaccat gaaaacgact tcgacgtcga tatggacctg
    29521 agcgaatgga tgcgccaatg ggggcaggaa ggcgacgacg aacaagtcat ccgcggcact
    29581 ttggggcgtt tgctcttcgg cagtaacgat gtcgtgaaaa aagtgaaggt tctctccggt
    29641 ggtgaaaaag gccgtatgct ttacggcaaa ctgttgctgt tgaaacccaa tgtcttagtc
    29701 atggacgaac cgaccaacca tatggacatg gaaagcatcg aatccttgaa catggcactg
    29761 gaaaaataca acggcacgct gatttttgtc tcccacgacc gtcagttcgt ttcctccttg
    29821 gcaacccaaa tcatcgaact ggacggcaaa ggcggatatg aacactactt gggcgattac
    29881 gaaagttact tggagaaaaa aggcgtagca taaccgccgg ttggaacaat gccgtctgaa
    29941 gccgcttcag acggcattgt tgataacttt aaaataggaa gcatatgcag acttacctcg
    30001 tcggcggcgc cgtccgcgat tatcttttgg gcttgcccgt caaagaccgc gattgggtgg
    30061 tcgtcggcgc agacgcacaa accatgctgg cgcaaggctt ccagccggtc ggcaaagatt
    30121 ttcccgtgtt tctccatccc gaaacgcacg aagaatacgc cctcgcccgc accgagcgca
    30181 aaaccgccaa aggttacgtc ggtttcagtt tccacgccga caaagacgtt acgctggagc
    30241 aggacttgat gcgccgcgac ctgaccatca acgcgatggc gcaagatgcg gacggcaaga
    30301 ttatcgaccc tttcggcgga caacgggatt tggcggcagg cattttgcgc cacgtttccc
    30361 cggccttcgc cgaagacccc gtccgcatcc tgcgtactgc ccgctttgcc gcgcgttaca
    30421 ggtttgaaat cgccgaagaa accataaagc tgatgcggca gatggtggaa aacggcgaag
    30481 cggacgcatt ggttgccgaa cgcgtctggc aggagtttgc gaaaggtttg atggaaaaaa
    30541 atccgcgcaa aatgattgaa gtgttgcgcg aatgcggcgc gctcaaagtc ttgctgcccg
    30601 aagtcaatgc cctcttcggc gtgccgcaac gcgccgacta ccatcccgaa atcgacagcg
    30661 gcatccatac cctgatgacg ctgcaacgcg ccgccgatat gggtttgagc ctgcccgaac
    30721 gctatgccgc cctgctgcac gacttgggca aagccaaaac accgtccgac atcttgccgc
    30781 gccaccacgg acacgacctc gccggtgtcg aacccgtgcg cgaagtcaat cagcggctgc
    30841 gtgcgccgaa acattgcgcc gagcttgccg aacttgtctg ccgttggcac atcatcttcc
    30901 atcaggttga aaaactccgc agcaaaacca ttatcgacgt attgaaaaaa accgatgcct
    30961 tccgccgccc agaacgtttt cagacggcat tgaacgtctg cattgccgac acgcaaggcc
    31021 gtctgaaccg cgaacacacg ccctacccgc aacgcgcgca ctggctcgcc ttactcgaag
    31081 ccgccaatca ggcggattcg ggcaaaatcg ccgccgaatg ccgcgcgcag ggaaaagcgc
    31141 accttatcgc cgaacaaatc gaccgggcgc ggctggcaca aatcgcccca ttgcaaaaag
    31201 cgtttcgagc ggcgcaagac aaaacagaaa aacattaaaa cgtccaatgc agccactttt
    31261 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg
    31321 gcaaggcgag gcaacgctgt a
//
LOCUS       DAWHXS010000010        27465 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWHXS010000010 DAWHXS010000000
VERSION     DAWHXS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.010"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(365..2140)
                     /gene="ptsP"
                     /locus_tag="WIE22_000362"
     CDS             complement(365..2140)
                     /gene="ptsP"
                     /locus_tag="WIE22_000362"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ4126507.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLENEIAKIVRCEDE
                     EKAEKLIKQINSVSVEEEADFKGRK"
     gene            complement(2140..2409)
                     /locus_tag="WIE22_000363"
     CDS             complement(2140..2409)
                     /locus_tag="WIE22_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ4126508.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2478..2915)
                     /locus_tag="WIE22_000364"
     CDS             complement(2478..2915)
                     /locus_tag="WIE22_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ4126509.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2996..3559)
                     /locus_tag="WIE22_000365"
     CDS             complement(2996..3559)
                     /locus_tag="WIE22_000365"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ4126510.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3628..4452)
                     /locus_tag="WIE22_000366"
     CDS             complement(3628..4452)
                     /locus_tag="WIE22_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ4126511.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     IGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            4544..5176
                     /locus_tag="WIE22_000367"
     CDS             4544..5176
                     /locus_tag="WIE22_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ4126512.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGSHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5244..7034)
                     /gene="rmuC"
                     /locus_tag="WIE22_000368"
     CDS             complement(5244..7034)
                     /gene="rmuC"
                     /locus_tag="WIE22_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ4126513.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKSRFQGVLAVLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYEHLQDENYALGNRFSAAEKQIAYLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(7373..8161)
                     /locus_tag="WIE22_000369"
     CDS             complement(7373..8161)
                     /locus_tag="WIE22_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ4126514.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8176..9525)
                     /locus_tag="WIE22_000370"
     CDS             complement(8176..9525)
                     /locus_tag="WIE22_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ4126515.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9544..10125)
                     /gene="petA"
                     /locus_tag="WIE22_000371"
     CDS             complement(9544..10125)
                     /gene="petA"
                     /locus_tag="WIE22_000371"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ4126516.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10248..10997)
                     /locus_tag="WIE22_000372"
     CDS             complement(10248..10997)
                     /locus_tag="WIE22_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ4126517.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11128..12057
                     /locus_tag="WIE22_000373"
     CDS             11128..12057
                     /locus_tag="WIE22_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ4126518.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(12312..12704)
                     /gene="rpsI"
                     /locus_tag="WIE22_000374"
     CDS             complement(12312..12704)
                     /gene="rpsI"
                     /locus_tag="WIE22_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ4126519.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12714..13145)
                     /gene="rplM"
                     /locus_tag="WIE22_000375"
     CDS             complement(12714..13145)
                     /gene="rplM"
                     /locus_tag="WIE22_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ4126520.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13323..13502)
                     /gene="ssrS"
                     /locus_tag="WIE22_000376"
     ncRNA           complement(13323..13502)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WIE22_000376"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13508..13813)
                     /locus_tag="WIE22_000377"
     CDS             complement(13508..13813)
                     /locus_tag="WIE22_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ4126521.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMATLNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13824..14153)
                     /locus_tag="WIE22_000378"
     CDS             complement(13824..14153)
                     /locus_tag="WIE22_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126522.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14208..15197)
                     /locus_tag="WIE22_000379"
     CDS             complement(14208..15197)
                     /locus_tag="WIE22_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ4126523.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEERE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15320..18022)
                     /gene="ppc"
                     /locus_tag="WIE22_000380"
     CDS             complement(15320..18022)
                     /gene="ppc"
                     /locus_tag="WIE22_000380"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ4126524.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVTELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18065..18958
                     /locus_tag="WIE22_000381"
     CDS             18065..18958
                     /locus_tag="WIE22_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ4126525.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADFDTVELHNLQRQVAFNEGDVGKLKTEALADRLRHINHTVDVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(19358..19582)
                     /locus_tag="WIE22_000382"
     CDS             complement(19358..19582)
                     /locus_tag="WIE22_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ4126526.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
     gene            complement(19583..20971)
                     /locus_tag="WIE22_000383"
     CDS             complement(19583..20971)
                     /locus_tag="WIE22_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ4126527.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(21057..21878)
                     /gene="prmC"
                     /locus_tag="WIE22_000384"
     CDS             complement(21057..21878)
                     /gene="prmC"
                     /locus_tag="WIE22_000384"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ4126528.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(21885..22328)
                     /locus_tag="WIE22_000385"
                     /pseudo
     CDS             complement(21885..22328)
                     /locus_tag="WIE22_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22393..23877)
                     /gene="tldD"
                     /locus_tag="WIE22_000386"
     CDS             complement(22393..23877)
                     /gene="tldD"
                     /locus_tag="WIE22_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ4126529.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      24055..24158
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24225..25448
                     /gene="cytX"
                     /locus_tag="WIE22_000387"
     CDS             24225..25448
                     /gene="cytX"
                     /locus_tag="WIE22_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ4126530.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            25445..26545
                     /locus_tag="WIE22_000388"
     CDS             25445..26545
                     /locus_tag="WIE22_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ4126531.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSLARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALVDGKDAPERDEESGLAYIRRQD"
     gene            26566..27183
                     /gene="thiE"
                     /locus_tag="WIE22_000389"
     CDS             26566..27183
                     /gene="thiE"
                     /locus_tag="WIE22_000389"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ4126532.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         5884 a         7742 c         7338 g         6501 t
ORIGIN      
        1 atggcggcat ttatcgggag caactgaaac caccctgccg tcattcccgc aaaagcagga
       61 atctagaacc caacacggca aaaatttatc cgaagcgaca acaatctttt catcgtcatt
      121 cccgcgcagg cgggaatcca gaacgtaaaa tctaaagaaa ccgttttacc cgataagttt
      181 ccgtgccgac agacctagat tcccgcctgc gcgggaatga cggattttag gtttctgatt
      241 ttggttttct gtttttgagg gaatggccga ttttgggttt ctgtttcggt tttctatttt
      301 gcaagaatgg caaaatttca gattgcgggc attgttaagt atttctattt tttacctgcc
      361 gtatttattt ccgccccttg aagtcggctt cttcctcgac agacacgctg ttgatctgtt
      421 tgatcagctt ttccgccttt tcctcgtcct cgcaacggac gattttggca atctcgtttt
      481 cgagctgtcc gacattgctg tgcagaatga tgtttttgac gggcaggatg ttgttggggt
      541 tcatggaaaa acggcgcagc cccataccca ataaaacgcg ggtaaacgcg gtatcgcccg
      601 ccatctcgcc gcatacggat acgtctttgt ccatgcggtt ggcggtacgg atgacgtgtt
      661 gcagcatttt cagcacggcg ggatggccgg gctggtagag gtggctgacg ctgtcgtcgc
      721 cgcgatcgac ggacaagatg tattgaatca ggtcgttggt accgacggag atgaaatcga
      781 ccagtttcaa aatactgccg acggtcagcg cggcagacgg aatttcaatc atacagccga
      841 tgccgacttt accgaaggca tcgccgcgtt cggcaagctg gcgttgcgcg gtgtcgaggt
      901 ggatgaggca ctggcgcact tcggatactg aggtaatcat cggccacatc atccgcacgg
      961 ggccgtgtac cgccgcacgg aggatggcgc gcatctgggt gcggaacatg accggttcgg
     1021 caaggcacag gcggatgccg gtcatgccca gcgcggggtt gaggctgccg ttgggcgtgc
     1081 tgtttttccc gaaccagcgc gggtttttgt ccacacccaa atcgactgtc cgtatcgtta
     1141 cgcttttgcc tttcattttt ttgacaatcg cgctgtacac ttcgtactgc tcgtcttcag
     1201 acggcatcgt atcgcggttc aggtaaagaa actcgctgcg gaacagcccg atgccgtctg
     1261 cgccgaggtt gtgcagcggt ttcacgtctt cggcggattc tatattgccc acaagctcga
     1321 tgcagacccc gtcggcggtg gcggcggcgg tttttttgag cttgttcaaa tcgcgtttgt
     1381 ggctgcggta ttcgcgggca cggcggcggt attcgttcaa caccgactca tccggcgcga
     1441 taatcaacac gccgttgata ccgtccacaa tgaccgtttc gccttcggta atcagtttgc
     1501 gcgcgttgtg cagcccgacg acggacggga tgtccaagct cctgcccaaa atcgccgtat
     1561 gcccggtggg gccgccggca tcggtaacga aggcggcaat gcgctgctct ttaaacaaaa
     1621 ccgtgtcggc gggcgaaagg tcgtttgcaa tcagaacggt ttcgtcaaac aggttgtcgg
     1681 caacttccaa ctcgttgccc tgcccgatca ggttgttgtg gatgcggcgg acgacttgca
     1741 gcatatcctg cttgcgttcg cgcaaatagg catcgtccat attgtcgaat tgggcggcga
     1801 gtttgtcgct ctgctgcttc aatgcccact cggcgttgat tttttgttcc cttaaaatat
     1861 cgacgggttc gcgcgacaag gtaacatcgg tcaagagcat caggtgcagc gagatgaacg
     1921 cgcccaactc ggtcggggcg ttttcgggaa tcgcgctgcg gagctgttcc aactctttgc
     1981 gcgtggcttt gacggcggca tcgaaacgtt cggcttcggc atcggtgtcc gcctccgcaa
     2041 catcatactg cggcacttcc tccgtaccgc gcgcaatcag gtgggcgcaa ccgacggcaa
     2101 tgcctttgcc cgccgccacg ccgtgcagca cgatactcat tattcgccct cgccgaagta
     2161 gccgttgatt aagtcggtca gggcgcgcat cgcttccgcc tcgtccgcgc cgtccgtctc
     2221 cagttcgatg accgtaccct tggcggcggc gagcatcatc agccccataa tgcttttgcc
     2281 gttgacgcgg ctgtcgtttt tcgtaaccca gacttcgctt ttgaattggg acgcggtttg
     2341 ggtgaacttg ttggacgcgc gggcgtggag tccgagtttg ttgatgattt cgatggattg
     2401 tttgagcatt tcggttcccg tgttatgtat atcggcagca gacgccgttt aaaatgtttt
     2461 cctgccctgc cgcttcttca gacggcatcg ccgctgcgcc ggcacaccaa atcttcgggc
     2521 gcggacgtga tggcgaaaat gccttttaca gccgcctccc tgacgcattc ggtaaaggcg
     2581 gcaaggtctt ccgccgccgg cgaatattgg acggccttaa ccatcatcgg cgcgttcagc
     2641 ccggtcaaaa tcgccgattt gttttcgcgc acgaggcggc gggctgcatt gcagggggtc
     2701 gcgccgaaaa tatcggtcat aattagcacg ccgtcgttgt cgggaaactc ctgaagcgcg
     2761 gcaatggcgt tgttgatgat gtcgtcttgg tcttccgtcg gctgcacgcc gagtatgcgg
     2821 acgttttcag gcagtccgcc cggaaaaaaa tgatgtgcca gcttgcggta ggcttcgcct
     2881 atggtttcgt gtgtgatgat taaaagccct atcatattat gcgtcctgtt cctcgttgtc
     2941 ctgccggcgt atgggcgcaa tgccgtctga acagccttca gacggcatcg cgtccttatt
     3001 ttccgtccaa tgcgtaaatc tcgcccaaat tgcgccagca gcccgccgca tccatgccgt
     3061 aaccgaaaac ataacggttc ggcacatcca gtccgacata atcggctcgg ataggcttgg
     3121 gtttgtcaat cagcttgttg gcgaacaccg ccgcacggca gcttgccgca cccatttcca
     3181 aaagtttggc ttgaatggcg gacatcgtat gcccttcgtc caaaatatcg tccagcacga
     3241 cgacgtgcct gccccggatt tgttccgcat cgggcatacg cttccagttg aacgcgccgc
     3301 cctccagctt gtcgccgtaa cgggaaacgt gaacataatc aaaatctaag ggaaaacgca
     3361 acagcggcag caactgcccc gtaaacacca ccgcgccgcc catcacgggc agcagcagcg
     3421 gatatttgcc gcccaaatcg cgcgtaatct cgtccgccac tttttgcagt gcggcacggc
     3481 attggccttg gtcgaacaaa agatcggcgt tttcaagcat cgcctgtgtt tcaaggcgtt
     3541 tggtttctaa atcggtcata tgtcggaatc ggtcggtaaa aggaaaatta taaaccaaag
     3601 tatcggatgc cgtctgaacc gtcctgctca gcggtcggta cgcacgcgca caaatgtggc
     3661 aaatttcggc gtgcctttcc gcgtaaaacc acggtagcgg taggtaatca gtgtgccgat
     3721 tttgggcggg ttgtcgcggt ctttgtcttt gaaaccgctg ccgatgcgaa attcgccgtg
     3781 ccggtttttg cagccgatcg cgcccaaccg tccggcgttt cgccctttgc cctcatagtg
     3841 ccgcgttacc gtgcattcgt cgtcgtattg gcttttcagc ttcaataatt ggctgctcct
     3901 gccgccgctg taacgggatt cgggctgacg cagcatcacg ccttcgccgc cctgcgcttc
     3961 gatttgtttt aaaaagtcca tcgcgtgctg ccggtcgcgc actttggttt gcgggatgat
     4021 ggtaatcggc gcgttcggat gcgttttcag ccactgcgtt gcgactgcca aacgttggta
     4081 gagattgccc tgcgccttgg gtacatcgaa aacgtgcagg cggatgccgc gccagtctga
     4141 agaaacagaa cgcacggtag cggaaatctg ctcgaactga ccacgtccgc tatacaattc
     4201 gccgtccaaa ggataaggcg gaaactgcgc ggtaaagcct ttggggggcg taaacgcgta
     4261 gccctgacgg cttatcaggt gctttccgtc ccaataggcg cgcacgccgt cgagtttctc
     4321 gctcattgcc cagccggcaa tatcctgccc tttgtattcc tgcgccagca tcaaatccgc
     4381 cgcgcctgcc gatgcaggga tgaaaaccgc cgtaaaaatc ggtatgatgc cgccgattgt
     4441 cttcttaatc atctgattcc ccaaatatca aaacaggcgg caaaccgcca taaaacaaac
     4501 ggcaaacccg atgccgtctg aaaaaccgtt taggaacacg ccgatgaccc tacgttacga
     4561 aatcttcccc gttaccccct tccgccaaaa ctgcaccctg atttgggacg acgaaagcgg
     4621 cgaagccgtc ctgaccgatg tcggcggcga cgtgccgttc ctgctgcaag cgttggcaaa
     4681 ccgcaaactt acgctcacgg caatctggct gacgcacggc catctcgatc acgcgggcgg
     4741 cgtggtcgaa atgttgaaaa cgcataaagt ccctgtcctc ggttcgcacc gcgaagatga
     4801 attcctgctc caatcgctgc cgcaaaccac cgcgcaatac ggatttcccg tctcgcccgc
     4861 ctttgcgccg aaccgttggc tcgaagaagg cgaaacgctc aaagtcggac gctatgcctt
     4921 tcaagtgctg catattccgg ggcacacgcc gggacacgtg gttttctatt gtgccgaagc
     4981 ggagctgctg attgcgggcg acgtgctgtt ttacgaaacc ataggcagaa ccgattttcc
     5041 gcgcggcaac cacgccgact taatcaataa tatccgcaac aaattattcg ccctccccga
     5101 aaccgtgcaa gttgtcgccg gacacgggcg tatgacttcc atcggacacg aaaaacggca
     5161 caatccgttt ttctaaccga cttcactacg gtcttcagac ggcgtcatct gcactgatgc
     5221 cgtctgaaac gcatccgcct ttcctattcg gcttcgtcag ccgcttcctg ttccgaaggt
     5281 ttgcccaaag caaatgccgt ctgttcgttg gcacgctcga ccaaatcccg ttgaagctgt
     5341 ttgcctgcct tcacgcccaa cagacgcagt ttctcggcgc ggccgaccag attcccgcgc
     5401 ccctcggcaa gttgcttgaa tgccgtctga aagctgtttt gcgcctgatc gatgcctttg
     5461 ccgacgcttt cgagcgtctg tacaaagccg acaaatttgt cgtacagctt gccgccttcg
     5521 tccgcaatcg ccagcgcgtt ttggttttgc tgctcgttgc gccaaatatt cgccaccgtc
     5581 cgcaaagtcg ccagcagcgt actggggccg accagcataa tccgtttgtc gaaacactct
     5641 tggaacaagc cagcgtcatt ctgcaacgcc aacaggtagg ccggttcgac agggataaac
     5701 ataaagacga aatccaatgt gttcacacct tccaaatcgg tgtaatcctt cagcgacaag
     5761 cctttcatgt gtgcacggat gctggcaacg tgtgccgcca gttcgcgtgc cgccgtatcc
     5821 gcatccgccg cctgcgtgta gcgcacataa gctgtcagcg agaccttgga atcaatcaca
     5881 atctgcttgt tgtcgggcag gttgaccaaa acgtcgggct ggaggcggcg cgtgccgccg
     5941 tcttcctctt ttcggacgga tgccgcctga accacatatt cgcgcccttt ctggaggccg
     6001 gaattttcca aaaccgtttc cagaatcatt tcgccccaat tgccctgaac cttgttctgc
     6061 gtaccggtca gcgcgttggt cagtgccttt gcctcgctgt gcagctgcgc attcaatcct
     6121 tgaagccgtt tcaattcgtt ttccaacgtc agccgctcgc gcgattcttt atcataggtt
     6181 tgcttgacca actcgccgaa accgtggatg cgttcgttca gcgggttcaa aacctgatgg
     6241 agctgctccc ggttctgctc ggtaaaacgg cggctttttt cttccaaaat cgtgttagca
     6301 agattttgaa actgatcgct caaactcttg cgcgcctcgc ccagcaagga cagcttctct
     6361 tcagaagcaa ggcgttcctg ttcgatttgc gttgccaaac gttcgttttc aaccgccaaa
     6421 ccctgtgcct tttcctgcaa ctcggtatgc gactgcctca accgctccgc ttccgcctct
     6481 ttttcctgca aatgggcaat ctgtttttcg gctgcggcaa aacggttgcc cacatcggaa
     6541 aggtcgtttt gcacgtcgcg gacagtttgg cggctttctt ccaaatcggt ttcgactctt
     6601 tggcggattt ggcgttccag ggcatattgg tcggcaaatg ccttccgctc ctgtccgagc
     6661 tgcgttgcca aacgttcgtt ttcaaccgcc aaaccctgtg ccttttcctg caactcgata
     6721 tacgactgct tcagacgcgc cgactccgcc tctttttcct gcaaataagc aatctgcttt
     6781 tcggctgcgg aaaaacggtt gcccaaagca taattttcgt cctgcaaatg ctcgtatttc
     6841 ccatccaaaa cggccaattc cgatgcaatt tctgcgtgtg cctgttcaac aaaatcacat
     6901 cttgccgcct tttccgccaa ttgcgcgttc aatacggcta atacaccttg aaaccggctc
     6961 ttcatcagta accatgtaaa caacacgccc gacaccaacg ccgccaaagg cagcaaaaca
     7021 gtcatcagtt ccatcaatca tcctaatatt caaacatttt cacaccggac aaaaccgccg
     7081 cttattccga ttctacctgt ttgttcgaca tagctccaaa aaatatagcg gattggcttt
     7141 aaacctgttc gacatcgcct taccatgctg cttgcggttt cagacctttt cctaattcaa
     7201 tatcaatctg ccacaaaccc tgattaagtt cccgatgtct gacattttta gaatgatgcc
     7261 gtctgaaatg ttgcagctat gttcagacgg catacggatt caggcttttc aaacggcagg
     7321 caaaatgaaa aaagggcaaa ccctaaagga ttttcccttt tgttccaaac gcttagtgta
     7381 cgtctttcca atattctttt ttcaggaaat acgccaaagg cagcataacc gcaaatagga
     7441 aaatcatcac gacatagcct atacgtttgc gttgcagttg tgcaggttcg cccatgtaca
     7501 caaggtaatt gaccaaatcg cgtacatatg cgtcgtactc tttttggatc actttgccgt
     7561 taggcagacg gcggctgtgc aaaccggtag attcccaata cagcttaggc ttcatctcgc
     7621 cgtgttcgtc ttttaccata accggctgac ctttggcatc caactcaacg gcttgaacgc
     7681 cttgctgttc ccacaacggg tggggcatac cgactttatc gaatacagta ttgttccagc
     7741 cgctcggacg ggtcggatct ttatagaagc cgcgcatata agcgtaaagg tagtctgcac
     7801 ctttggaacg cgcaatcaat gtcaaatcgg gcggagcagc accaaaccat tttgccgcat
     7861 ctttcgggtt catcgccgaa tgcatgacat cgccgacatt atcggtggta aacatcaggt
     7921 ttttcttgat ttcttcgtca gtcaaaccga tgtctttcaa acggttgaaa cgcataccgc
     7981 ttgccgagtg gcaggacagg caatagttgg taaagatttg tgcgccgcgc tgcaggctga
     8041 cttggtcgcg caaatcgata tcgactttct catagtgtgc atgaccgccc gaagcaaatg
     8101 ccgcacccat gggtactgcc aacagcaagg cagcaaacca atttttcata gcttgtttca
     8161 ttttgactgc cctcatcaga tgttggttgc aaacaggtaa gcacccacgg cggtaatgcc
     8221 gacgtaaaca aagaacatga ttttttgttt ggcggtgctc atggttacgc gttcgggaac
     8281 cggtttgttt ttatccagct tggtatagaa cggcataccc aggaagaatg cgaaatagac
     8341 gaaggacagg atgcgcgcga ccaaagtacg cgtatcggtt gccaccatcg cgcccaaaat
     8401 acccaaaccg ataaaggaaa tgatgaacaa gaccaatgcg gttttgaaaa tcgggccgcg
     8461 gtaacggacg gatttcacct cgcctctatc cagccaaggc agcagggcaa tcaacacgac
     8521 cgccgcgccc ataccgatta caccccaaac ctgcgttccc aagaacgagg gaatcgcacg
     8581 caaaatggcg tagaacggtg tgaaatacca taccggcgca atgtgcggag gtgttttcag
     8641 cgcatttgcc gcatcgaagt tcggcgcttc gaggaagtag ccgccgcctt ccggtgcgaa
     8701 gaacaatacg gcacagaaga caatcaggaa tacaaccaca cccaagatgt ctttaacggt
     8761 atagtaagga tggaacggga taccgtcgcg cgggataccg ttttcgtctt tgtttttctt
     8821 gatttcaacg ccgtcagggt tgttagaacc cacttcgtgc agggcgatga tgtgcgccac
     8881 aaccaagccg agcaatacca gaggtaccgc gataacgtgc agggcaaaga agcgattcag
     8941 ggtaacgtcg gaaacgttga agtcgccgcg aatccaagtg gacaaatccg gaccgataac
     9001 agggatggcg gagaacaggt taataattac ctgcgcaccc caaaaggaca tctgacccca
     9061 aggcagcagg tagcccataa aggcttccgc catcaatgcc aagaaaatca gagaaccgaa
     9121 aatccacacc aactcacggg gttttttgta cgaaccgtaa atcaaaccac ggaacatgtg
     9181 cagataaaca acaatgaaga agaaagatgc gccggtggaa tgcatgtagc ggataatcca
     9241 accgccggac acgtcgcgca tgatgtactc gactgcagta aaggcagcag gcagatggta
     9301 ggcattcagg ttaccgtccg gtttgtagtt catcgtcagg aaaataccgc tgacgatttg
     9361 aatcacgagg acaagcaagg ctaaagagcc gaagtagtac caaaaattaa agtttttcgg
     9421 tgcgtagtat tgagccaaat gctcattcca cattttagac agggggaaac gagcgtccat
     9481 ccagcctaac aatgcttttg ctttgctatt ggtttggttt gccataatta tcgttcctta
     9541 ttcttagtct tcgcccacca agatagttgt gtcgctcaag tatttatatg gcgggacaac
     9601 caggttggtc ggggcaggaa cacctttata tacgcggccg gccaagtcga atttcgaacc
     9661 gtggcagggg cagaagaagc cgcctttcca gtctgcaccc aaatcggcgg gggcaatgtc
     9721 gggacggaag gtgggcgagc agcccaaatg ggtgcagata ccgatggcga caaggatgtt
     9781 cggcttaatc gaacgggtct cgtttttagc atactccggc tgctgttccg catcggaatt
     9841 gggatcggta agttcgccgt tcaggccttt caggtcttta agctgctgat ctgtacggtt
     9901 gagcacccaa atcggtttgc cttgccactc ggcggtcagc agctgacccg cttcgatttt
     9961 actgacatcc acctcgacgg cagcaccggc ggccttggct ttttccgaag ggaaaaaact
    10021 ggccacaaac ggcgttgcca cacccaatgc tgccactccg cccgcgccgc aggtcgcgag
    10081 tgtcaggaaa cggcggcggc cgttgttgat ttcttgatta tccattattc agtcgtccta
    10141 atattttggg aataccgagc cattaaacgt tgcaatttta cccagtttgc agtgatactc
    10201 aaagcattat ttaaaataag gtaaagtttt atgatatttc tcaagactca agccggattg
    10261 ttttcgtcaa aatggcacac ttccaacccg aaaacctctg ccgccgattc tgccagcgcg
    10321 cgtacgccgt aacgttccgt cgcgtgatgc cctgccgaaa tgaaagccgt acccgtttca
    10381 ttggcaaggt ggtattgagc ttcagagatt tcccccgtca aatacagatc gacaccttcg
    10441 tctattgccg tctgaaaaaa cccctgcgcc ccgccgctgc accatgcaac ccgtcggatt
    10501 tcgcgttcgg gtttgccgat gacgacaggc ttacgttgca aaactgtttc aatatgcgcc
    10561 gccaatgcgc cgagtgtctt ggcttgtttc aggctgcccg agttgagcag gttttgttcg
    10621 ccgaaccgtt tttctgtcgc aaaacccaat ctgtcggcga gttgggcatt gttgcccagt
    10681 gtgggatgtg catccagggg cagatggtag cctgccatat tgatgtcgtg ccgtaacagt
    10741 gcggcaatcc gttctttttt ccaaccagta acggtcggca actcgttttt ccagaacata
    10801 ccgtgatgta ccaaaagcaa atctgccttc tgctccacag caaaatcaat cgctgccctg
    10861 cttgccgtta ccgacgtaac gattttccct atatattccc tcccttcaac ctgcaaaccg
    10921 ttaggggcgt aatctttaaa caacgctgtc tgcaatgttt cattacacca agtcagaaaa
    10981 tccctgcgca ataccatctt ttttcctaat cgctttaaac aagcgggcat tctaatcgca
    11041 aaatgtccgg aattcacatt tttccgattt gcacccgcat atgaattatt ttaatatgcg
    11101 ccggttcaat atgccgtctg aagccccatg gattccatta tcgaattgcg ccacctcaaa
    11161 accctgctgg cacttgaaga aaccggcagc gtctcccttg ccgccaaacg ggttttcctt
    11221 acccaatccg ccctttccca ccagatccgt atgctcgaaa accactacgg cacgccgctg
    11281 ttcgaacgca aatccacgcc cttgcgcttt accccggtgg gcgaaaggct gctgcgcctc
    11341 gcccacgaac tgatacctca agttgctgtt gcagaacgag atttggcgcg aatcacagaa
    11401 ggggaggcgg gagagctgcg gattgccgtc gaatgccata cctgtttcga ctggctgatg
    11461 cccgccatgg gcgaattccg cccgatgtgg ccccaagtcg aattggatat cgtatcggga
    11521 ttccaagcgg atcccgtcgg actgctgctg caacaccgtg ccgaccttgc cattgtttcc
    11581 gaagcggaaa aacaaaacgg tatttctttc caaccgctgt ttgcctacga aatggtcggc
    11641 atttgcgcac cagaccatcc gcttgccgcc aaaaacgttt ggacggcgga agactttatc
    11701 ggggaaaccc tgattactta tcccgttccc gacgagatgc tggatttgcc caaaaaaatc
    11761 ctgattccga aaaacatcaa cccgccgcgc cgacacagcg aactgaccat cgccattatc
    11821 caactggttg ccagcagacg tggcattgcc gcccttccct attggacagt catgccctac
    11881 cttgaaaaag gctatgtcgt tcaccggcag attaccgacg acggactgca aagcaaactg
    11941 tatgctgcca tccgcactga agatacggac aagagctatc tgaacaactt ttgccaaatc
    12001 atacgtgaac gcggttttgc agatttgccc ggactgagcg aactggaatc ggtctgaccc
    12061 cttattcaac catacccggc agtttttcta ttttttcatg tatagtggat taacaaaaac
    12121 cagtacagcg ttgcctcgcc ttgccatact atttgtactg tctgcggctt cgtcgccttg
    12181 tcctgatttt tgttaatcca ctatactgtt tttgattttt gcccaatctg taatctttag
    12241 attgccaatg gggaaccgtc tactacaaat aaaaaaccct gcgataagca gggttttttg
    12301 aatttccaac attaacgttt ggagaattgt tttgcacggc gtgctttgcg cagacccggt
    12361 tttttacgtt cgacttcgcg ggcatcgcgg gtaacaaaac cagcttgaga caaggcgggt
    12421 ttcaacgcgg catcgaagtc gatcagggca cgggtaatgc cgtggcggat tgcgccggac
    12481 tggccggttt cgccgccgcc aacaacattg actttgatgt cgaaagattc ggcgttttca
    12541 gtcagaacca agggttggcg aacaaccatt cggctggttt cccgtgcgaa gaattcgtca
    12601 acgggacgac cgtttacgat gatttgacct gtacctttaa tcaggaatac acgagccact
    12661 gaacttttgc ggcggcctgt gccgtagtag tatttaccgt tcatgtcgcg tccttatttc
    12721 agttccaaaa ctttgggttg ttgcgcagca tgggcgtgtt ccgcacccgc atacactttc
    12781 agttttttaa tcatggcgta acccagagga cctttgggca gcataccttt tacagcttgt
    12841 tccaaagcgc ggcccgggaa ttgctcttgc atttcgcgga aggtgcgttc gtagataccg
    12901 cctgggaaac cggaatggcg gaagtatttt ttatcttcga atttggcacc ggttacacgc
    12961 agtttgtccg cattgataac aatgatgtaa tcgccggtat cgacgtgggg ggtgtattca
    13021 ggtttgtgtt tgccacgcag acggctggcg acttcggccg caacgcgacc caagactttg
    13081 tcttgggcat cgatgacgaa ccattcgcgc ttcacctcgt ggggtttcgc tgaaaaggtt
    13141 ttcatagtgg aaatccagat agatatagaa agttgtaaat tttaaagaca ggattcgatt
    13201 ttgtcaatcg cattaccgcg ttacggaagg attttccgga tttcggcaga ctgcatactg
    13261 ctttttcggg cggggcggcg gccaatgtga aaaaccgcat cgttgcgatg cggttttgaa
    13321 tgggaatccc cgcgagagcc gtttcggccg aatccgcttg aaccttgctg acaaggcggc
    13381 tgcctcgggt agtttcgggt gcgtccgcaa aaggacgctc gcgcccacta ctgctcccgg
    13441 caaccttaag cgaacttatt ggttcaaagg aatatatgcc ttcgcggaca ccgcagggaa
    13501 aaaggggtta ttcctgcgcc aagcgggata gtgctttttg gcaggcgttg tccatatcgg
    13561 ctattttacg cgcaaaatcg ccgattgcca aatcgccgcc gttcagggag gttttcaaca
    13621 ggtcgtggac gacgttgagc gtggccataa tgacgatttt ttcgctgtcc gcgacgcgtc
    13681 cgccttcgcg gatggcttcg gctttgccgt tgagcattcc gactgcctgc aacagtgtgt
    13741 ctttttcttc tgccggcgtg ttgacggtca gccgggcgtg catgacttcg atgtggactt
    13801 gttcgatgtt catcctttaa tccttattgc tgcgtttcct gccattgggg gaggcgcgct
    13861 gccagtgcgc tgattttttc cctgctctgt tcgagcaggc tgcggtatcg tgtattttct
    13921 tctgtcaggc tgtcgatttt gttttgcagg tcttctttga gtttgccgac ttggacgagc
    13981 agggcttcgc tgagttcgtc gacggcggtt tcgtgttcga gtttttgccg ctcgtgcgcc
    14041 cgtttgagtt cggcgacggt ttctttgagg cggcggtttt cgctgacgag ggtttcgaat
    14101 ttttgtacca gcgtataaac gctgctttcg agtttttcga tattttgttt cataacctta
    14161 cctgtccgta tgccgtctga aggcctcaga cggcatctgt ctgttgttta ttcgaaacgc
    14221 gcgctgcgtt ccatcagtct ttcgacgacc tgctgagggg tcatttcttt gcggatgagt
    14281 tgcagcaggg tttgggtaat cggcatgtcg atttggtact tacaggcagt attgaagact
    14341 tcttctatcg tgctgacccc ttcggaaacg tgtccgattt cgaccagcac ctgatgcagt
    14401 tccttgcctt ctgccaaacc caagccgacg cggcggttgc gtgagagtgc gccggtgcag
    14461 gtcaagatga ggtcgccgat gccagcaagc cccatcatgg ttttgggctg tgcgcccatt
    14521 gcggaggcaa ggcgggtgat ttcggccagt ccgcgtgtaa ccagtgcggc acgggcgtta
    14581 agcccgtact ccagaccgtc ggacaatccg gtggcaatcg ccatgacgtt tttgactgca
    14641 ccgccgactg ccacgccgat aacgtcggta ctgccgtaaa gcctcatgac ggtcgtgttg
    14701 agctgcggta cgagttcttc aatccactct tggttttcgg aggcgaggac gacggcgcag
    14761 ggcagttgtt tggcgagttc ctgtgcaaaa ctcggaccgg aaagtacgcc gattttttta
    14821 ttgtcgggca atacttcttt caatacttga aaggtcagca gccctgtatc ctgctcgaat
    14881 cctttgcagg cggcgaggac ggggaggtgt cccgcgccgt actgtttgag caactctgcg
    14941 ctgcttctca atccggcaac ggaggttacg ataaggacaa gtccgctgtc tttgagcgcg
    15001 tctgccaaat ccgcacacac ttccaaggtt tcgggaaagg aaaagccggg cagtccgcgt
    15061 ttgttttcac gttcttcctg catttggcgg acttggtctg cgttgcgcgt ccacagggat
    15121 acgcggttgc cgtgttggga aaaatgcagg gcgagcgccg taccccacga acctgcgccg
    15181 ataacggtaa ttttcattgg tcgtctttca acatatcact gccgttcact ttaaaacaat
    15241 cggtgtttct ctgcaagtgc ggtcagggaa atgccgtctg aaaggcgttc agacggcatt
    15301 ttgccccgat gcggcactat cagcctgtat tgcgcaaacc ttgcgccacg ccgttgatgg
    15361 tcaggtgcac catcagaagg gcgtgcggat tgtcgggttc tttacgcagg cgtttgagca
    15421 tggcgacttg caggccgttg agggcgttca aatatggaat cctcaaggca agcgaacggg
    15481 cgaggctgcg gttgtcgcgc aggagctctt cggtttgcag caagtcgagc agtgctttgc
    15541 ggctgcgttg gtattcttcc ttgatcatgc cgaagatgac ttttgctttg tcgggtgatt
    15601 cgctcaagcc ggcatagttt tccgccaagg tgatgtcggt tttcgccatc acttgttcca
    15661 tattggagag catggcttgg aagaacgggt tgctttgggc atgttcgcgc agggcggcga
    15721 gggtttcggg tttgtcttcg cacaaggttt ctaccgcgct gccgaaaccg taccaagccg
    15781 gcagcatgag gcggttttgc atccaggaga atacccacgg aatcgcgcgc aagtcctgaa
    15841 tccgcgccaa ggttttacgg ctggcgggac ggctgcctag gttgagggtg gcgatttcct
    15901 gaatcgggct ggtttgcaga aagtagtcga tgaagtcggg atgggtaatc agttcgcggt
    15961 agtatttgaa cgatacgtcc gacaatgcct gcatcagttt ggcatcaggg tcttttttat
    16021 ccggcaggat gctggcttcc aaagtcgcgg caaccaaggt ttccaagttg cgttgggcat
    16081 tgccggggtc ggcgtatttg gcggtaatga cttcgccttg ttcggtaatg cggatttgtc
    16141 cggcaacgct gcccgccggt tgggcgagaa tggcttggta agaagggccg ccgccgcgtc
    16201 cgacgctgcc gccgcgaccg tggaacaggc gcatgcggac atcgtatttt ttgaagagtt
    16261 cgaccaagcc caattccgcc tgatacagac accatgagct ggtaacatag ccgccgtctt
    16321 tgttggagtc ggagtagccg agcataattt cttggatgtt gccgcggctt tcgagcaggg
    16381 catcgtacca atcgaggcgg aacatggttt ccatgaccgg acaggcgttt tcgagtgctt
    16441 cgatggtttc aaacagcggc acgatattga tgcggctgtg cggtttgccg ttttccaccg
    16501 ccaacaggcc gctttctttc agcagcaatg ccaaggcgag caggtcgctg ggttgttcgc
    16561 agttggaaat aatgctttgt gttacggcat cttcgccaaa ttcgtctttg attttgcgcg
    16621 cttcgttgaa aattgccagt tcgtggcggg tatggtcgct gtatgtgatg aacgggctgt
    16681 acagcggacg ttgatggctc aattcgcgca acaggacggc ttgtttttgt tcttcgttca
    16741 ggctgttgta gtcttccaag cctgcgtgtt ggaaaagctc ggtaaccaca tcggcgtgtt
    16801 tgcctgcgtg ttggcgcagg tcgagcggca tcatgtggaa gccgaacacg gacacggaac
    16861 ggatgaggtc tgccaaacgg ccttcggcaa gcaggcggct gccgttgtcg ataagggaac
    16921 gttgcaattt tttcaaatca tcgagaaatt cttgtgccga agcataaggc tcgagaaagc
    16981 cgaatttgca gcccatgccc aaaccgagcg cgcgcgcttt gcccatagcg cgcgccataa
    17041 tgtaggcaat ggcgcggcgg tagggttctt ccgtgcgggc aagttcttcg tcaggcgaga
    17101 gggctgccag tgccattacg tcgtcgttga ctttgacgcg gcggatggag agcggcaatt
    17161 cgcggtagag tttgtcgagt tcgccacggt aaaaacggaa cacggcatcg gcgtggcggc
    17221 ggaaggcaaa gcgcagggtt tcggcagaaa cgaacggatt gccgtcgcgg tcgccaccga
    17281 tccagccgcc gattttgagg atgttcggaa cgcggacatc gggataggcc gtctgaaagt
    17341 cgtgttccat cttgcggtag agtttgggca gggcttcaaa aaagctcatc gggaagatgg
    17401 acacgccgtt gttgatttcg tcgttgacgc tgagtttgtg gcggcgcgtt tcgctggtct
    17461 gccacaaacc cagcaggata gtgtcgattt cgcggcgcag ccgtgccagc gcgtcggcat
    17521 tggtgcagcg ttcgcgttgc ggcaacagtg cgcggatgcg gcggttgaag cttaagacgg
    17581 tttggcgttg cacttcggtc gggtgcgcgg tcaaaacggc ggtaacggac gtattgtcca
    17641 actgccgctg caccgatttg ccgtcggctt tccccgcttt gagcctgcgg acggtttccg
    17701 tcaggctgcc ttccgcgccg ttgtggccgg catcttcgtg gatttggcgg cggcgttcgt
    17761 ggtgcacgtc ttcggcgatg ttcaaaatct gggcgaacag gccgcaggcc aaggttaaat
    17821 cgtgggtttg ttgttcgtcc aattgcggca atactttttc aatcaatgcc gcgctgtcgt
    17881 cggaagtgga caagagtttg actgtttcga caaccaacgg cgaggcttct tcgtgcagga
    17941 ggttgaacag ggattgtttc agaaattccg cgtccgccgc caaagccgcg tccttcggat
    18001 tgttcagaat atgcagttgc atgatttttc tctcattgcc gtaaatactg taaatgtacc
    18061 tcaaatgccg catccgtgcc aaaccgttca cactttaacc gcccgtgtcc cgaaatgccg
    18121 tctgaagttg aacgccgccc gacggcagcg ttacaatcgc ccgcaactgt tttttccgaa
    18181 catcatcatg accacgaccg aacacgacaa cgacgacgaa ctcctgctgc ggtacagccg
    18241 ccacatcctc ttggacgaaa tcggcatcga agggcagcaa aagctttccg acgcacatat
    18301 tttggtcgtc ggctgcggcg gattgggtgc cgccgcactg ccctaccttg ccgcttcggg
    18361 tgtcggcacg ctgaccatag ccgatttcga cacggtcgaa ctgcacaacc tgcaacgtca
    18421 ggtcgcattt aacgagggcg acgtcggcaa actcaaaacc gaagccttgg cagaccgtct
    18481 gcgccatatc aaccataccg tcgatgtccg cgccgtcaac gaaaaactcg acggctgccg
    18541 cctgaccggt ttggttcaag ccgccgacat cgttttagac tgttgcgaca actacgccac
    18601 gcggcaagcc gtcaaccgtg cctgcgtgca aacgaaaaca ccgctggttt caggggcggc
    18661 ggtacgcttt gaagggcaac ttgccgtgta ccgtcccgac ttgcccgact cgccgtgtta
    18721 cgcctgcctg tttgacggcg gatcggcttc agacggcatc tgttccctct tcggcgtgtt
    18781 ctcgccgctg gtcggcatca tcggcagtac ccaagcggcg gaggctctga aaatcctgct
    18841 ggatgcgggc gaaccgtcgc acggcaggct ggcggtttac cgtgccttgg aagggggctg
    18901 gcaatatttc gacctgccgc gcaaccccga atgcccgatt tgcggcgcgg aacggtaaaa
    18961 ccctgccgcc gtttcagacg gtatccaaac ggatgcggcg gaacggtttt aaaaatttaa
    19021 aaatttacat ttctttgcaa aaaaaaataa acttacctta taattgcagt tgttttagca
    19081 atgtctgttt cgcagactca ttgagtaaga cgttttcccc gtaatgtgtt tggccgtctg
    19141 tcccctttgg gttcggacgg ctttttttag gctgtgtttg aatacccggt tggtttcgtc
    19201 tgtttgcagg ggaaaaccgc ttatttccgt tcgggcggaa aacggttcca tcggataaaa
    19261 ggcattttgt ccgactgatt aaagttacat cttaggtttg catcggcaaa atattcaaac
    19321 acagcctttt ttaaggaaat ccggatacgg cggcacatca ataatgcggc ggaatctcgt
    19381 cgcgcaggga atacggctct tgcgtgtcgg gattcctgtc ctgcattttt tgatacagca
    19441 gcctcaactg agcctgctgc aaatccagcg tctgccgcaa ttccgccacc atcgcgttca
    19501 ggccggcgat tacgtcctcc tgaagcgcgg attggatttc cagctctgtg atgcggtgtt
    19561 ccaactcttg aaccgcgtcc atttacagca ccatcgcggc aatccagccg gcaatcagca
    19621 gcgggatatt gtagtggatg aaggtcggga taacggaatc gcggatgtgg tcgtgctgcc
    19681 cgtcggcgtt cagccccatc gtcgggccca gcgtggaatc ggacgcaggc gaaccggcat
    19741 cgcccaacgc ccccgccgtg ccgacaatgg cgacggtggc aagcggcgaa aaacccaaac
    19801 cgacacacaa aggcacataa atcgcggcaa taatcggcaa agtggaaaag gacgaaccga
    19861 tgcccatcgt taccaaaagc cccaccacca gcatcgccaa tgccgccata cctttgctgt
    19921 tgccgaatat cgccatactg ctttccacca gcggctggat atgcccggtc gcattcatca
    19981 cggcggcaaa accctgcgcg gcaatcataa tgaagccgac catcgccatc atcttgatac
    20041 cttcgccgaa tacgtcgttt gccttgtcgc ggttgatgac ccccaacatc ataaatacgg
    20101 cgaaaccgag catcgcgccc aacaccagcg agtcttcata catcaactgg atggcaaaac
    20161 atacggcaat ggcggcggcg gcggccaggc tgcggtaggc ggacggctgc ggacggtttg
    20221 ccgcatcggc gttgcccgcc gtatcggcat tgttgctttg gtacaggcgc ggtttgcggt
    20281 aatggacaaa cgccagcagg agtccggcca gcattcccaa cgcgggaatc gccattgccg
    20341 ccatcacgtt aatgtttttc acatcaagct gcggcgcggc ggaatggatg ttgcccaaca
    20401 ggatttcgtt caaaaaaatc gcgccgaagc cgtaaggcag gaacatataa gtcgtaacca
    20461 gcccgaaagt gatgacgcac gcaatcaggc ggcggtcgat tttcaggcgg ttgaacacca
    20521 aaagcagcgg cggaacaatc atcgggataa aggcaatgtg gatggggatg atgttctgac
    20581 tcatcatgcc catcacaagg atgatggaaa gcagcagcca tttgaccgcg ccctcgcccg
    20641 aacgcacgct gtcgggcata ccgccccggt tcagtttgcg gacgaccgcg ccggcaagct
    20701 gctgcggcag gccggaatgg gtaatcgcca tcgcaaacgc accgagcatc gcataagaaa
    20761 gcgcaatctt cgcgccgcct tccaaacctt tgttgaacac ggggataatc cccgcctgac
    20821 tgacctgtcc cgccgcatcg gcaatgtttt gcagcggcat acccgccacc gcgccgccga
    20881 caaacgcgcc gaccgtcagg ctcaatacca cgtgcacgcg cgacagcgac agcaccagca
    20941 taacgattac ggcaactacg actgcattca ttgtttatcg ctccaaacct ataaatgttt
    21001 acatatcgaa acacatcata acccaataac ggaaaacccg ccaattttgc aaacaattat
    21061 ttcaaatgct tcatatactt ccccagcgta accctgtcca aacccgccaa gtccggcagg
    21121 gtttccactc ccgaaaaacc attctccgcc aacacgccgc gcaccgccgc gccctgatcg
    21181 aaaccgtgtt ccagcaataa aaaaccgcct tccgccaaac ggtcgggcgc gccttgcgcc
    21241 aaggtacgga tgcagcttag gccgtctgaa aagtcggtca gcgcgatttg cggctcaaac
    21301 cgcaaatcgc cttgcgacaa atgtttatcg ccgttttcga tatagggcgg gttggacacg
    21361 atgatgtccc atttcccttc agacggcata tcggtgtcga accacgaacc gtgtgcaaat
    21421 tcgacccgcg cgcccaaatc cgccgcattt ttccgcgccg tttcaagggc gggcgggctg
    21481 atgtcggatg cgcgcacaaa cgcatcgggg cgttcgagcg cgacggttac ggcaaccgcg
    21541 ccgctgcccg tccccaaatc ccacacgcgc ccgttttcgg gcaggcgcgc caatacggct
    21601 tcgaccaagt gttcggtttc ggggcgcgga atcagcacgc tcggattgac tgtaaagcgt
    21661 ctgccataaa attcgcgcac acctaaaata taggcaaccg gctcgccgtt cagacggcgt
    21721 tgcgccagcc tgtccgcccg ctgtcggact tcgtccggca tttcttcccc gccccgcgtc
    21781 aacaactgca cgcgcgtata ttccgaaaca tattgcagca gcattcttgc ttcattttta
    21841 ggcagttttg acaagcccaa ccatttatca aacgtcattt ttatcccgtc tgccgctgat
    21901 gcggcttttc tttccttatt ctttccggca aacgtaccga tggtggcaac cgcaaatgcg
    21961 gcataccaca aataaaatcc tgcaccgtag cgcacaatat ccgatgtatt ccctgcttca
    22021 tcgacgtata cggctttcac actgaaagcc accaacgcca agccccaaag tgccgcatgg
    22081 acaggcacga ccttcttccg caacgccagc aaaacaatgg ccgccaacca aacataattc
    22141 gcatagaccg cacaatacct gatatccaaa gaagcaaata tcgaccccaa aatcaaaacg
    22201 gtcaaaccct ccatgcttcc atgattgccc aaataaaatg caacattgga taaagacgct
    22261 atccacaggg caaccgacac cagcaacatc actatgggaa aacttgattt ccgattctgt
    22321 tcctgcatgg ttttatccta atgtaaaagg ccgcctgaaa acctttcaga cggcatcgtg
    22381 ccggattccg cgtcagattg cgctgccgcc gacggtcagc ccggcatcga tacgcagggt
    22441 cggctgcccc acgccgacgg ggacgctctg cccttctttg ccgcacacgc cgacaccgct
    22501 gtccaacgcg gtatcgttgc ctatcatcga aacgtgtttc agcacttcgg gcccgttgcc
    22561 gataatggtc gcgcctttga cggggtattg cagcctgccg ccttccaccc accatgcttc
    22621 ggacgcgctg aacacaaatt tgccgctggt aatgtccacc tgtccgccgc caaagttgac
    22681 ggcgtaaatg cctttgtcga tggacgcgat gatttcttcc ggctcatagc tgccattttc
    22741 cataaaggta ttggtcatgc gcggcatcgg gacggaggcg tagctttcgc ggcggccgtt
    22801 gccggtggat tgcgtacccg tcaggcgggc attggtttcg tcctgcatat agccgactaa
    22861 aatgccgtct tcaatcaata cggtgcggcg ggtttcgttg ccttcgtcgt cgatgttgag
    22921 cgaaccgcgc cggccggcaa tatcgccctg atcgacgacg gtaacgcctt tggcggcgac
    22981 gcgctcgcct attctgccgg aaaagacgct ggtccccttg cggttgaaat cgccttccaa
    23041 accgtgtccg accgcttcgt gcagcaacac gcccggccag ccgttgccca aaacgacggt
    23101 catttcgccg gcgggcgcgg ggcgggattc gaggttggtg agtgcctgtt tgacggcggc
    23161 atcgacaaac tgccgcacca agttttcatc gaaataagcc aagtcgtagc gtccgccgcc
    23221 gcccgcgctg ccctgttcgc ggcgttcgcc ctgtttggcg atgacggtaa cgttcaggcg
    23281 caccatcggg cggatgtcgg cggcgtgttt gccgtccaga cgggcgaggt aaaccatatc
    23341 gtattcgcag gtcaaaccgg ccatcacttg cacgatgcgc ggatcggcgg ctttggcgat
    23401 tgcttccact ttgttcaaca gcgcgacttt ggcggcggaa tcgaggccgg caatggggtc
    23461 ggacgcggaa caaaccggct tgccgcgcgt ttcagacggc attttggcgg acaccttgcc
    23521 gcctgccgcc ccaatcgcgc ggacggcgcg ggcggaacgg tttatcgaat cgatgcacag
    23581 gctgtcggcg taggcaaagg cggttttatc gcccgaaacg gcgcgcacgc ccacgccctg
    23641 attgatttgg aagctgcccg atttgacgat gccctcttcc aaatgccagc tttcataagc
    23701 ggtgcgctgg cagtagatgt cggcgtaatc gacgtggtgc gcgccgatga tgcacaggct
    23761 tttggcgagc agttcggggg aaaggcggtt ggcttcgagc agccgcgcct gtacggcgga
    23821 ataggtcgga tgcatagtgt cggcgcataa aaaatcaggg gcttgattat acggcatttg
    23881 ttatatagtg gattaacaaa aaacagtacg gtgttgcctc gccttgccgt actatttgta
    23941 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataga aatgcgccgt
    24001 gccgcctgaa atgtaagatt tttgccaacg ccccctgctt ttgtgtacac ttaaagctcc
    24061 ttgtcggagt gccgccgccg ggcggctgag attgcgaaag cagaatccgt agaacctgtc
    24121 ggggtaatgc ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc cgttcccttt
    24181 tccgcacttc cccgcccacc ttccttcttt ttaaaggacg tcatatgtcg ggcaatgcct
    24241 cctctccttc atcttccgcc gccatcgggc tgatttggtt cggcgcggcg gtctcgattg
    24301 ccgaaatcag cacgggtacg ctgcttgcgc ctttgggctg gcaacgcggt ctggcggctc
    24361 tacttttggg tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat atcggcgcac
    24421 tgaccggacg cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc ggttcagtgc
    24481 tgttttccgt ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg atttacgccg
    24541 gcgcaacggt cagctccgct ttgggcaaag tgttgtggga cggcgaatct tttgtctggt
    24601 gggcattggc aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca cgcaaaacag
    24661 gcgggctgaa aaccgtttcg atgctgctga tgctgttggc ggttctgtgg ctgagtgccg
    24721 aagtcttttc cacggcaggc agcaccgccg cacaggtttc agacggcatg agtttcggaa
    24781 cggcagtcga attgtccgcc gtcatgccgc tttcctggct gccgctggcc gccgactaca
    24841 cacgccaagc acgccgcccg tttgcggcaa ccctgacggc aacgctcgcc tatacgctga
    24901 cgggctgctg gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga gaaaccgacg
    24961 tggcaaaaat cctgctgggc gcaggtttgg gtgcggcagg cattttggcg gtcgtcctgt
    25021 cgaccgttac caccactttt ctcgatgcct actccgccgg cgtaagtgcc aacaatattt
    25081 ccgccaaact ttcggaaaca cccatcgccg ttgccgtcgc cgttgtcggc acactgcttg
    25141 ccgtcctcct gcccgttacc gaatatgaaa acttcctgct gcttatcggc tcggtatttg
    25201 cgccgatggc ggcggttttg attgccgact ttttcgtctt gaaacggcgt gaggagattg
    25261 aaggctttga ctttgccgga ctggttctgt ggctggcggg cttcatcctc taccgcttcc
    25321 tgctctcgtc cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg tctgccgttg
    25381 ccattgccac cgtatcggta cgccttttct ttaaaaaaac ccaatcttta caaaggaacc
    25441 cgtcatgacc cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga ccgcactgca
    25501 gcttgcagaa caaggttatc agattgcact tttcgataaa ggctgccgcc ggggcgaaca
    25561 cgccgccgcc tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg tcgaagccac
    25621 gcccgaagtg gtcaggctgg gcaggcagag catcccgctt tggcgcggca tccgatgccg
    25681 tctgaacacg cacacgatga tgcaggaaaa cggcagcctg attgtgtggc acggacagga
    25741 caagccatta tccagcgagt tcgtccgcca tctcaaacgc ggcggcgtag cggatgacga
    25801 aatcgtccgt tggcgcgccg acgacatcgc cgaacgcgaa ccgcaactcg gcggacgttt
    25861 ttcagacggc atctacctgc cgaccgaagg ccagctcgac gggcggcaaa tattgtctgc
    25921 acttgccgac gctttggacg aactgaacgt cccttgccat tgggaacacg aatgtgcccc
    25981 cgaagacctg caagcccaat acgactggct gatcgactgc cgcggctacg gcgcaaaaac
    26041 cgcgtggaat gcttctgccg aacacggcag caccctgcgc ggcatacgcg gcgaagtggc
    26101 gcgggtttac acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc atccgcgtta
    26161 tccgctctac atcgccccga aagaaaacca cgtcttcgtc gtcggcgcga cccaaatcga
    26221 aagcgaaagc caagcccctg ccagcgtgcg ttccgggctg gaactcttat ccgcactcta
    26281 tgccgtccat cctgccttcg gcgaagccga catcctcgaa atcgctaccg gcctgcgccc
    26341 cacgctcaac caccacaacc ccgaaatccg ttacagcctc gcccgacgcc tgattgaaat
    26401 caacggcctt ttccgccacg gcttcatgat ttcccccgcc gtaaccgccg ccgccgtcag
    26461 attggcagtg gcactggttg acggaaaaga cgcgcccgaa cgtgatgaag aaagcggttt
    26521 ggcgtatatc cgaagacaag attaaagccg tctgaaagga accccatgac cttcccgccc
    26581 ttgaaatccc cgctcaaatt ctacgccgtc gtccccaccg ccgattgggt ggggcgcatg
    26641 gtcaaagcag gtgccgacac ggtgcaactg cgctgcaaga ccctgcacgg cgatgaattg
    26701 aaacgcgaaa tcgcccgctg cgtcgccgcc tgtcaaggca gccgtacgca gcttttcatc
    26761 aacgaccact ggcgcgaggc aatcgaagca ggcgcatacg gcgtgcatct cgggcaggaa
    26821 gatatggaca ccgccgacct cgccgccatt gccgccgccg gtttgcgctt gggtttgagt
    26881 acgcactccg tcgccgaact cgaccgcgcc ctgtccgtac accccagcta tgtcgccagc
    26941 ggcgcgattt tcccgaccac gaccaagcaa atgcccaccg ccccgcaagg cttggacaaa
    27001 ctgtgcgagt atgtgaaaca ggcaggcggc acgcccgtcg tcgccatcgg cggtatcgac
    27061 ttgaacaacg cccgagccgt actcgccacc ggcgtttcct cactcgccgc cgtccgcgcc
    27121 gtaaccgaag cggcaaatcc cgaagcggtg gttaaagcgt ttcaggcttt gtgggatgga
    27181 taaaaccgaa agaagaaaat tcaattgccg tgtaggcaaa acttagcccg ttatcgcaaa
    27241 catacttaac tttaaatgtg gcatatcatc aaattccgtc attcccgcgc aagcgggaat
    27301 ccgccttaaa acttgagaaa ccatcatttg aaaaacagtt tccgaatttc aaaaatggat
    27361 tcccgcctgc gcgggaatga cggcaaccgg ccggttgcgt atcaaaaaat aaagtaattc
    27421 ggctagatat agtggattaa caaaaatcag gacaaggcga cgaag
//
LOCUS       DAWHXS010000011        27441 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWHXS010000011 DAWHXS010000000
VERSION     DAWHXS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.011"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            25..1107
                     /locus_tag="WIE22_000390"
     CDS             25..1107
                     /locus_tag="WIE22_000390"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ4126533.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1222..1494
                     /locus_tag="WIE22_000391"
     CDS             1222..1494
                     /locus_tag="WIE22_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ4126534.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            1584..2921
                     /locus_tag="WIE22_000392"
     CDS             1584..2921
                     /locus_tag="WIE22_000392"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ4126535.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLSPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            3047..4372
                     /locus_tag="WIE22_000393"
     CDS             3047..4372
                     /locus_tag="WIE22_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ4126536.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4376..5443
                     /gene="murG"
                     /locus_tag="WIE22_000394"
     CDS             4376..5443
                     /gene="murG"
                     /locus_tag="WIE22_000394"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ4126537.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5598..7007
                     /locus_tag="WIE22_000395"
     CDS             5598..7007
                     /locus_tag="WIE22_000395"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ4126538.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            7121..8035
                     /locus_tag="WIE22_000396"
     CDS             7121..8035
                     /locus_tag="WIE22_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ4126539.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            8025..8753
                     /locus_tag="WIE22_000397"
     CDS             8025..8753
                     /locus_tag="WIE22_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ4126540.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8839..10083
                     /gene="ftsA"
                     /locus_tag="WIE22_000398"
     CDS             8839..10083
                     /gene="ftsA"
                     /locus_tag="WIE22_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ4126541.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            10202..11380
                     /gene="ftsZ"
                     /locus_tag="WIE22_000399"
     CDS             10202..11380
                     /gene="ftsZ"
                     /locus_tag="WIE22_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ4126542.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11519..12829
                     /locus_tag="WIE22_000400"
     CDS             11519..12829
                     /locus_tag="WIE22_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ4126543.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            13455..14330
                     /gene="prpB"
                     /locus_tag="WIE22_000401"
     CDS             13455..14330
                     /gene="prpB"
                     /locus_tag="WIE22_000401"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ4126544.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            14416..15570
                     /gene="prpC"
                     /locus_tag="WIE22_000402"
     CDS             14416..15570
                     /gene="prpC"
                     /locus_tag="WIE22_000402"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ4126545.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            15731..16519
                     /locus_tag="WIE22_000403"
     CDS             15731..16519
                     /locus_tag="WIE22_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ4126546.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLTDERNPLYQMIISMF"
     gene            16597..19203
                     /gene="acnD"
                     /locus_tag="WIE22_000404"
     CDS             16597..19203
                     /gene="acnD"
                     /locus_tag="WIE22_000404"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ4126547.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKTQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            19555..20724
                     /gene="prpF"
                     /locus_tag="WIE22_000405"
     CDS             19555..20724
                     /gene="prpF"
                     /locus_tag="WIE22_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ4126548.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            21138..22337
                     /locus_tag="WIE22_000406"
     CDS             21138..22337
                     /locus_tag="WIE22_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ4126549.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            22696..23316
                     /locus_tag="WIE22_000407"
     CDS             22696..23316
                     /locus_tag="WIE22_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ4126550.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(23443..23886)
                     /locus_tag="WIE22_000408"
     CDS             complement(23443..23886)
                     /locus_tag="WIE22_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ4126551.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            24060..24428
                     /locus_tag="WIE22_000409"
     CDS             24060..24428
                     /locus_tag="WIE22_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ4126552.1"
                     /translation="MKPLKRHPALIGLSCDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            24473..25627
                     /locus_tag="WIE22_000410"
     CDS             24473..25627
                     /locus_tag="WIE22_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ4126553.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVLAWLSAVFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(25684..26556)
                     /locus_tag="WIE22_000411"
     CDS             complement(25684..26556)
                     /locus_tag="WIE22_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ4126554.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDQASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            26641..>27441
                     /locus_tag="WIE22_000412"
     CDS             26641..>27441
                     /locus_tag="WIE22_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ4126555.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSCEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVL"
BASE COUNT         6230 a         7098 c         7712 g         6401 t
ORIGIN      
        1 gacaaaacaa aaaaaggaag ccccatgttt ttatggctcg cacatttcag caactggtta
       61 accggtctga atatttttca atacaccaca ttccgcgccg tcatggcggc gttgaccgcc
      121 ttagcgtttt ccctgatgtt cggcccgtgg acgatacgca ggctgaccgc gctcaaatgc
      181 gggcaggcag tgcgtaccga cggtccgcaa acccacctcg tcaaaaacgg cacgccgacg
      241 atgggcggtt cgctgattct gaccgccatt accgtgtcca ccctgttgtg gggcaactgg
      301 gcaaacccgt atatctggat tctcttgggc gtattgctcg ccacgggcgc actcggtttt
      361 tacgacgact ggcgcaaagt cgtctataaa gaccccaacg gcgtgtccgc caaattcaaa
      421 atggtgtggc agtcaagcgt tgccattatc gccagtttgg cattgtttta ccttgccgcc
      481 aattccgcca acaatatttt gattgtcccg ttcttcaaac aaatcgccct gccgctgggc
      541 gtggtcggct ttttggtgtt gtcttacctg accatcgtcg gcacatccaa tgccgtcaac
      601 ctcaccgacg gcttggacgg ccttgcgacc ttccccgtcg tcctcgttgc cgccggcctc
      661 gccatcttcg cctatgccag cggccactca caatttgccc aatacctgca attaccttac
      721 gttgccggcg caaacgaagt ggtgattttc tgtaccgcca tgtgcggcgc gtgcctcggt
      781 ttcttgtggt ttaacgccta tcccgcgcaa gtctttatgg gcgatgtcgg tgcattggca
      841 ttgggtgccg cgctcggtac cgtcgccgtc atcgtccgcc aagagtttgt cctcgtcatt
      901 atgggcggat tatttgtcgt agaagccgta tccgttatgc ttcaggttgg ctggtataag
      961 aaaaccaaaa aacgcatctt cctgatggcg cccatccatc accactacga acaaaaaggc
     1021 tggaaagaaa cccaagtcgt cgtccgcttt tggattatta ccatcgtctt ggtgttgatc
     1081 ggtttgagta ccctcaaaat ccgctgaacc tatgccgtct gaacaccttt cagacggcat
     1141 ttgaacgcgc aataaacctg cggcgacaat ccgcccagcc ctatcgttaa cggcggctta
     1201 tactaaaaca gaagtaaaac catgaaacag acagtcaaat ggcttgccgc cgccctgatt
     1261 gccttgggct tgaaccaagc ggtgtgggcg gatgacgtat cggattttcg ggaaaacttg
     1321 caggcggcag cacagggaaa tgcagcagcc caatacaatt tgggcgcaat gtattacaaa
     1381 ggacgcggcg tgcgccaaga ccttgtgctc gctcaagaat ggtttggtaa ggcttgtcaa
     1441 aacggagacc aagacggctg cgacatttac caacgcctga aggcaggtta ttgaacagcc
     1501 cgcgatgccg tctgaaagcg gcttgggcag gggcggacat ccccccacct gcccaatatg
     1561 atttgtttta ggacaaaacc aaaatgacgc ttgaaaacaa aaaaatcctc gtcgccggac
     1621 tcggcggtac gggtatttcc atgattgcct atctgcgcaa aaacggcgcg gaggtggccg
     1681 cttatgatgc ggagttgaag cccgagcgcg tgtcgcaaat cggtaagatg tttgacgggc
     1741 tggtgtttta cacaggccgt ctgaaagatg cgctggacaa cggtttcgat attttggcac
     1801 tcagccccgg catcagcgag cgtcagccgg atattgaggc gttcaagcaa aacggcggac
     1861 gcgtgttggg cgacatcgaa ttgctggcgg acattgtgaa ccgccggggc gacaaggtaa
     1921 ttgcgattac cggcagcaac ggcaaaacca cggtaacgag cctggtcggc tatctctgta
     1981 tcaagtgcgg gctggatacc gttatcgcgg gcaatatcgg cacgccggtt ttggaggcgg
     2041 aatggcagcg cgaaggcaaa aaggcggacg tgtgggtgtt ggagctttcc agcttccaac
     2101 tggaaaacac cgaaagcctg agcccgacgg cggcgacggt actgaatatt tccgaagacc
     2161 atctcgaccg ctacgacgac ttgctcgact acgcgcatac caaagccaag attttccgtg
     2221 gcgacggtgt gcaggttttg aatgcggacg atgcgttctg ccgtgccatg aaacgtgccg
     2281 ggcgcgaggt aaaatggttt tcgttggaac acgaagccga tttttggttg gaacgtgaga
     2341 caggccgcct gaaacaaggc aatgaagatt tgattgccac gaaagacatt ccgttgcaag
     2401 gcttgcacaa cgccgccaac gtcatggctg ccgtggcttt gtgcgaagcc gtcggtttgc
     2461 cgcgcgaagc attgcttgaa cacgtcaaaa ccttccaagg cctgccgcac cgcgtggaaa
     2521 aaatcggcaa gaaaaacggc gtggtattca ttgatgacag caaaggcacg aatgtcggcg
     2581 cgaccgccgc cgcgattgcc ggtttgcaaa atccgctctt cgtgatttta ggcggcatgg
     2641 gcaaagggca ggacttcacg cctctgcgcg acgcgcttgc cggcaaggca aaaggcgtgt
     2701 tcctgatcgg cgttgatgcg ccgcaaatcc gccgcgattt ggacggctgc ggtctgaata
     2761 tgaccgactg cgccactttg gaagaagccg ttcagacggc atatgcccaa gccgaagcgt
     2821 gcgatatcgt gctgctcagc ccggcttgtg cgagttttga tatgtttaaa ggctacgcgc
     2881 accgttcgga agtgtttatc gaagcgttta aggctttgtg atgccgtctg aaatgcaaac
     2941 gccgtcattg ttgggcggca agtaaagatt tagaataccg atttgggatg tatcgtatgt
     3001 tcggacggca ttgtctgccg tctgaaattt ttgccctttg cggcaggtgc aaacagactg
     3061 gcaggtggtt tttttgaaga tttcggaagt attggtaaaa gtgggcgacg gtgtccacac
     3121 tctgctgctc gacagaccga ttgtgcgcga cggcaggaaa ttcgacgcgc cgcttttgtg
     3181 gatggtggtg ctgatgacgg cgttcagcct gctgatgatt tattcggctt ctgtgtattt
     3241 ggcatcgaag gaaggcggcg atcagttttt ctatttgacc agacaggcgg ggttcgtcgt
     3301 tgccggcttg atagcgagcg gttttttatg gtttctttgc aggatgagga catggcggcg
     3361 gcttgtgccg tggatttttg ccctatccgg cctgttgctg gtagtcgtat tgattgccgg
     3421 gcgcgaaatc aatggcgcga cccgttggat acctttgggt ccgttgaatt tccagccgac
     3481 cgagctgttc aagctggcgg tcatccttta tttggcaagc ctgttcacgc gccgtgaaga
     3541 agtgttgcgc agcatggaaa gtttgggttg gcagtcgatt tggcggggga cggccaatct
     3601 gatcatgtcc gccaccaatc cgcaggcacg tcgtgaaaca ttagaaatgt acggccgttt
     3661 ccgggcgatc atcctgccga ttatgctggt ggcgttcggt ttggtgctga taatggtaca
     3721 gccggatttc ggttcgtttg tcgtcattac cgtcattgcc gttggaatgc tgtttttggc
     3781 aggattgccg tggaaatatt ttttcgtcct ggtaggcagc gtcttgggcg ggatggtgct
     3841 gatgattacc gccgctccct accgtgtgca gcgggtagtg gcatttttgg acccgtggaa
     3901 agaccctcag ggtgccggct accagcttac ccactctctg atggcaatcg ggcgcggaga
     3961 gtggttcggt atgggtttgg gtgcgagttt gagcaaacgc ggctttctgc cggaagcgca
     4021 taccgatttt atttttgcca tcatcgccga agaattcggt ttcttcggta tgtgtgtgct
     4081 gatattctgt tacggctggc tggtggtgcg ggcgttttcc atcggcaagc agtcgcgcga
     4141 tttgggtttg actttcaacg cctatatcgc ttcgggtatc ggcatttgga tcggtatcca
     4201 aagtttcttc aatatcggtg tgaacatcgg tgctttgccg accaaaggtc tgacgctgcc
     4261 gttgatgtcc tatggcggtt cgtcagtctt tttcatgctg atcagcatga tgctgctgtt
     4321 gcgtatagat tatgaaaacc gccggaaaat gcgcggttat cgggtggagt aaatcatggg
     4381 cggtaaaacc tttatgctga tggcgggcgg aacgggcgga catattttcc ccgcgctggc
     4441 ggtggcggat tcattgcgcg cgcgcggcca tcatgtgatt tggctgggca gcaaggattc
     4501 gatggaagag cgtatcgtgc cgcaatacgg catacgcttg gaaacgctgg cgattaaagg
     4561 cgtgcgcggc aacggcatca aacgcaagct gatgctgccg tttactttgt atcaaaccgt
     4621 ccgcgaagcg cagcggatta tccgcaaaca ccgtgtcgag tgcgtcatcg gcttcggcgg
     4681 cttcgttacc ttccccggcg gtttggcggc gaagctatta ggcgtgccga ttgtgattca
     4741 cgagcaaaac gccgtggcag gtttgtccaa ccgccacctg tcgcgctggg cgaagcgggt
     4801 gttgtacgct ttcccgaaag cgttcagcca cgaaggcggc ttggtcggca accccgtccg
     4861 cgccgatatt agcaacctgc ccgtgcctgc cgaacgcttc caagggcgtg aaggccgtct
     4921 gaaaattttg gtggtcggcg gcagtttggg cgcggacgtt ttgaacaaaa ccgtaccgca
     4981 ggcattggct ttgctgcccg acaatgcgcg tccgcagatg taccaccaat cgggacgggg
     5041 caagctgggc agcttgcagg cggattacga cgcgctgggc gtgaaagccg aatgtgtgga
     5101 atttattacc gacatggtgt ccgcctaccg cgatgccgat ttggtgattt gccgtgccgg
     5161 cgcgctgacg attgccgagt tgacggcggc gggattgggt gcgttgttag tgccgtatcc
     5221 tcacgccgtt gatgaccatc aaaccgccaa cgcacgtttt atggtgcagg cggaggcggg
     5281 attgctgttg ccgcaaaccc agttgacggc ggaaaaactc gccgaaatcc tcggcagcct
     5341 gaaccgcgaa aaatgtctca aatgggcaga aaacgcccgt actttggcac tgccgcacag
     5401 tgcggacgac gttgccgaag ccgcgattgc gtgtgcggcg taaactgccg aaccatgccg
     5461 tctgaaaagc cgttcagacg gcatggatgt tttttatttc aatccgctat atatttgtca
     5521 gaaaactatg gcgcgcaaac ggtcagccct ttaaaataac gcctttacgc actaaaaacc
     5581 aaccggaacg caacattatg atgaaaaatc gagttaccaa catccatttt gtcggtatcg
     5641 gcggcgtcgg catgagcggc atcgccgaag tcttgcacaa tttgggtttt aaagtttccg
     5701 gttcggatca ggcgcgaaat gccgctaccg agcatttggg cagcctgggc attcaagttt
     5761 atcccggcca taccgcagaa cacgttaacg gtgcggatgt cgtcgttacc tctaccgccg
     5821 tcaaaaaaga aaatcccgaa gttgtcgctg cgttggagca gcaaattccc gttattccgc
     5881 gcgccctgat gttggcggag ttgatgcgct tccgtgacgg catcgccatt gccggcacgc
     5941 acggcaaaac cacgaccacc agcctgaccg cctccatcct cggcgcggca ggacttgacc
     6001 cgactttcgt tatcggcggc aaactcaacg ccgcaggcac caacgcccgc ttgggcaaag
     6061 gcgaatacat cgttgccgaa gccgacgagt cggatgcatc ctttctgcac ctgacaccga
     6121 ttatgtccgt cgttaccaat atcgacgaag accatatgga tacctacggg cacagtgttg
     6181 agaagctgca tcaggcgttt atcgatttca tccaccgtat gcccttctac ggcaaagcct
     6241 ttttgtgtat tgacagcgaa cacgtccgcg cgattttgcc caaagtgagc aaaccttatg
     6301 ctacttacgg tttggacgat accgccgaca tctacgccac cgacatcgaa aacgtcggcg
     6361 cgcaaatgaa attcaccgtc catgttcaaa tgaaaggaca tgagcagggg tcgtttgaag
     6421 tcgtgctgaa tatgcccggc agacacaacg tgctgaacgc attggcagcc atcggcgtgg
     6481 cgctggaagt cggcgcatcg gttgaagcga tccaaaaagg cttgctcggc tttgaaggcg
     6541 tcggccgccg cttccaaaaa tacggcgaca tcaagttgcc aaacggcggg accgcgctct
     6601 tggtggacga ctacggacac caccccgtcg aaatggcggc gacccttgcc gccgcacgcg
     6661 gcgcgtatcc ggaaaaacgt ttggtactcg ccttccagcc gcaccgctat acccgcacgc
     6721 gcgatttgtt tgaagacttt accaaagtcc tcaataccgt tgacgcgctg gtgctgaccg
     6781 aagtttatgc cgccggtgaa gagccgattg ccgccgccga ttcccgcgct cttgcccgcg
     6841 ccatccgcgt gttgggcaaa ctcgagccga tttactgcga aaacgttgcc gatctgcccg
     6901 aaatgctgtt gaacgttttg caggacggcg acatcgtgtt gaatatgggc gcgggaagca
     6961 tcaaccgcgt tcccgccgcg ctactggaat tgtcgaaaca gatttgaggc acaccctcct
     7021 gacagacgga acatcatata aaggtcgtct gaaaccgcaa atcaggtttc agacgacctc
     7081 tggcaacaag cataaagcaa tcaggaaaga acaaaaaaca atgcagaatt ttggcaaagt
     7141 ggccgtattg atgggcggtt tttccagcga acgagaaatc tcgctggaca gcggcaccgc
     7201 cattttgaac gctttaaaaa gcaaaggcat agacgcatac gccttcgatc ccaaggaaac
     7261 cccattgtct gaattgaagg cacaaggttt tcagacggca ttcaacatcc ttcacggtac
     7321 ttacggcgaa gacggggcgg ttcaaggcgc gttggaactg ttgggcattc cctataccgg
     7381 cagcggtgtc gccgcctccg ccatcggcat ggacaaatac cgctgcaaac tgatttggca
     7441 ggcattgggg ctgcccgttc ccgagttcgc cgtcctgcac gacgacactg atttcgatgc
     7501 cgtcgaagaa aaattgggcc tgccgatgtt tgtgaaaccg gcggccgaag gcagcagcgt
     7561 cggtgtggta aaagtcaaag aaaaaggccg tctgaaaagc gtttacgaag aattgaaaca
     7621 ccttcagggc gaaatcattg ccgaacggtt tatcggcggc ggcgaatatt cctgccccgt
     7681 cctgaacggc aaaggcctgc ccggcataca catcatcccc gcgaccgagt tttacgacta
     7741 cgaagccaag tacaaccgcg acgacaccat ttatcaatgt ccttcggaag atctgaccga
     7801 agccgaagaa agcctgatgc gcgaactggc ggttcgcggc gcgcaggcaa tcggtgcgga
     7861 aggctgcgtg cgcgtcgatt tcctcaaaga taccgacggc aaactctatc tgttggaaat
     7921 caacaccctg cccggtatga cgagccatag tttagtaccg aaatccgctg ccgttacggg
     7981 cgtgggtttt gccgatttat gtattgaaat tttgaagacc gcacatgtgg gataatgccg
     8041 aagcgatgga acggctgacg cgctggctgc ttgtcatgat ggcgatgctg cttgctgcgt
     8101 ccgggctggt ttggttttac aattcgaatc atctgcccgt caagcaggtg tcgctgaagg
     8161 gcaacctggt ttattccgat aagaaagcat tgggcagttt ggcgaaagaa tacatccatg
     8221 ggaatatttt gaggacggac atcaatggcg cacaggaggc ctaccgccgg tatccgtgga
     8281 ttgcgtcggt catggtgcgc cgccgttttc ccgacacggt tgaggtcgtc ctgaccgagc
     8341 gcaagccggt cgcgcgttgg ggcgaccatg ccttggtgga cggcgaaggc aatgtttttg
     8401 aagcccgctt ggacagaccc ggaatgccgg tattcagagg cgcggaagga acgtctgccg
     8461 aaatgctccg ccgttatgac gaattttcga ctgttttggc aaaacagggt ttgggcatca
     8521 aagagatgac ctatacggca cgttcggcgt ggattgtcgt tttggacaac ggcatcaccg
     8581 tcaggctcgg aagggaaaac gagatgaaac gcctccggct ttttaccgaa gcgtggcagc
     8641 atctgttgcg taaaaataaa aatcggttat cctatgtgga tatgaggtat aaggacggat
     8701 tttcagtccg ctatgcttcc gacggtttac ccgaaaaaga atccgaagaa tagtgggaac
     8761 aggtatcgga cagattacgg ccgtgccgtc tgaaacggtg cgacgcaaat ttcaatcagt
     8821 tttaagagca gacgaacaat ggaacagcag caaagataca tcagcgtact ggatatcggt
     8881 acgtctaaag tcctcgcact gatcggggaa gttcaagatg acgacaaaat caacatcgtc
     8941 ggtttggggc aggctccttc acggggcttg cgcgcgggca tggtaaccaa tatcgatgcc
     9001 accgtccaag ccatcaggca ggcggtcaat gatgccgagc tgatggcgga taccaaaatt
     9061 actcacgtta ccacaggtat cgcaggcaac cacatccgca gtctcaattc gcaaggtgtg
     9121 gttaaaatta aagatgggga agtcacgcag gcagacatcg atcgcgccat tgaaacggca
     9181 aaggcaatca atatcccgcc cgatcaaaaa attctcgatg ccgtggttca agactacatt
     9241 attgacaccc aacttggcgt gagggagccc atcggtatga gcggtgtgcg tctggatacg
     9301 cgggtgcaca tcattaccgg tgcaagtacg gcagtgcaga atgtccaaaa atgtatcgag
     9361 cggtgcggtt tgaaaagcga tcagatcatg cttcagccgt tggcaagcgg gcaggcggtg
     9421 ctgactgaag atgaaaaaga cctcggcgta tgcgtcatcg acattggtgg cggaacgacc
     9481 gatattgccg tttatatgaa cggtgccatc cgccatacgt ccgtcattcc ggccggtggt
     9541 aatctgatta ccaaagattt gtccaaatcg ttgagaacac ctctcgatgc cgccgagtac
     9601 attaaaatcc attatggcgt ggcatcatgc gatacggaag gcttgggtga gatgattgaa
     9661 gttccgggcg tgggtgaccg gacatcgcgt caggtttcca gtaaggtttt ggcagcaatc
     9721 atcagcgcac gtattcagga gatttttggc gtagtgctgg gcgagctgca aaaatcgggt
     9781 ttccccaaag aagtgctgaa tgcgggtatc gttctgaccg gcggtgtgtc catgatgacc
     9841 gggattgtgg aatttgccga aaaaatcttc gatttgcctg tacgcaccgg tgcgccccaa
     9901 gaaatgggcg gtttgtccga ccgcgtccgc acaccgcgct tttctaccgc catcgggctg
     9961 cttcatgcag catgcaagct ggaaggaaac ttgccgcagc cggaaaacgg tgcagtgcaa
    10021 gagagggaag ggggcggcgg tttgttggca agattgaaac ggtggattga aaacagcttc
    10081 tgaacaggtg gattgccgtt tgacaggtga gaagtatttt gccagcagca agatacttct
    10141 tatataatga ataataattt atttaaaccg tcctctgaat ggggcgagca ggagtttttg
    10201 aatggaattt gtttacgacg tggcagaatc ggcagtcagc cctgcggtga ttaaagtaat
    10261 cggcttgggc ggcggcggtt gcaatgcaat caataacatg gttgccaaca atgtgcgcgg
    10321 tgtggagttt atcagtgcca atacggatgc gcagtctctg gcaaaaaacc atgcggcgaa
    10381 gagaatccag ttgggtacga atctgacacg cggtttgggc gcgggcgcga atcccgatat
    10441 cggccgtgcg gcagcccagg aagaccggga agccattgaa gaagccattc gcggtgcgaa
    10501 tatgctgttt atcacgaccg gtatgggcgg cggtaccggt accggttccg cgccggttgt
    10561 tgctgagatt gccaagtctt tgggcattct gaccgttgcc gtggttaccc gaccgttcgc
    10621 atatgaaggt aagcgcgtcc atgtcgcaca ggcagggttg gaacagttga aagaacacgt
    10681 cgattcgctg atcatcatcc cgaacgacaa actgatgact gcattgggtg aagacgtaac
    10741 gatgcgcgaa gccttccgtg ccgccgacaa tgtattgcgc gatgcggtcg caggcatttc
    10801 cgaagtggta acttgcccga gcgaaatcat caacctcgac tttgccgacg tgaaaaccgt
    10861 gatgagcaac cgcggtatcg ctatgatggg ttcgggttat gcccaaggta tcgaccgtgc
    10921 gcgtatggcg accgaccagg ccatttccag tccgctgctg gacgatgtaa ccttggacgg
    10981 agcgcgcggt gtgctggtca atattacgac tgctccgggt tgcttgaaaa tgtccgagtt
    11041 gtccgaagtc atgaaaatcg tcaaccaaag cgcgcatccc gatttggaat gcaaattcgg
    11101 tgcggctgaa gacgagacca tgagcgaaga tgccatccgg attaccatta tcgctaccgg
    11161 tctgaaagaa aaaggcgcgg tcgattttgt tccggcaagg gaggtagaag cggttgctcc
    11221 gtccaaacag gagcaaagcc acaatgtcga aggtatgatc cgcaccaatc gcggtatccg
    11281 cacgatgaac cttaccgctg cggatttcga caatcagtcc gtacttgacg actttgaaat
    11341 ccctgcgatt ttgcgtcgtc aacacaattc agacaaataa tgtgctgttt gcccgtaaac
    11401 ctgctgcctc ccgaatcggt ttgtccggtt tgggaggtat gtttttcaag atgttgcaat
    11461 ttcgtacggt ttgcggtcgg cggattcaga tttttccact tgatacagac tttcagatat
    11521 ggacacttca aaacaaacac tgttggacgg gatttttaag ctgaaggcaa acggtacgac
    11581 ggtgcgtacc gagttgatgg cgggtttgac aacttttttg acgatgtgct acatcgttat
    11641 cgtcaaccct ctgattttgg gcgagaccgg catggatatg ggggcggtat tcgtcgctac
    11701 ctgtatcgcg tctgccatcg gctgttttgt tatgggtttt gtcggcaact atccgattgc
    11761 actcgcaccg gggatggggc tgaatgccta tttcaccttt gccgtcgtta agggtatggg
    11821 cgtgccttgg caggttgcgt tgggtgcggt gttcatctcc ggtctgattt ttatcctgtt
    11881 cagctttttt aaagtcaggg aaatgctggt caacgcactg cctatgggtt tgaaaatgtc
    11941 gattgctgcc ggtatcggtt tgtttttggc actgatttcc ctgaaaggcg caggcattat
    12001 cgttgccaat ccggcaacct tggtcggttt gggcgatatt catcagccgt ccgcgttgtt
    12061 ggcattgttc ggttttgcta tggtggtcgt attgggacat ttccgcgttc aaggcgcaat
    12121 catcatcacc atcttgacca ttaccgtcat tgccagcctg atgggtttga atgaatttca
    12181 cggcatcatc ggcgaagtac cgagcattgc gccgactttt atgcagatgg attttgaagg
    12241 cctgtttacc gtcagcatgg tcagtgtgat tttcgtcttc ttcttggtcg atctatttga
    12301 cagtaccgga acgctggtcg gcatatccca ccgtgccggg ctgctggtgg acggtaagct
    12361 gccccgcctg aaacgcgcac tgcttgcaga ctctaccgcc attgtggcag gtgcggcttt
    12421 gggtacttct tccaccacgc cttatgtgga aagcgcggcg ggcgtatcgg caggcggacg
    12481 gaccggcctg acggcggtta ccgtcggcgt attgatgctc gcctgcctga tgttttcacc
    12541 tttggcgaaa agtgttcccg cttttgccac cgcgcccgcc ctgctttatg tcggcacgca
    12601 gatgctccgc agtgcgaggg atattgattg ggacgatatg acggaagccg cacccgcatt
    12661 cctgaccatt gtcttcatgc cgtttaccta ttcgattgca gacggcatcg ccttcggctt
    12721 catcagctat gccgtggtta aacttttatg ccgccgcacc aaagacgttc cgcctatggt
    12781 atggattgtt gccgtattgt gggcactgaa attctggtat ttgggctgat tgattcgata
    12841 ttaaaaatgc cgtctgaaag gttttcagac ggcattttgt ttgccgatat atttaatttt
    12901 tattaaatta tataaaaatc aaatatataa taaaatacat cggattgctt aaaaataata
    12961 cattgttttt atgtataaaa tattttataa gttttcagga ttttgattat cgaaaatttt
    13021 tcttgatttc ctgacaattt tattgaaaca aataattcaa aattaatcta gtttaatcat
    13081 ggaattaaaa taaaatatta aaattatgta atgagtctcc ttaaaaatgt ttgacatttt
    13141 cagtcttgtg ttttagatta tcgaaaaata aaactacata acactacaaa ggaacattac
    13201 tatgaaacca attcagatgt tttccccttt tctgaataat ccccttgttt tcttcttgtc
    13261 tgcggttttg ccgcataatt ccgaacggtc tgctgttttt ctttgattcg ttttaaatat
    13321 caataagata atttttccca tatattttta atgattggat tgggatgccc gacgcgtcgg
    13381 atggctgtgt tttgccgtcc gaatgtgatg gaagcctgtc catactgaaa aaaagtctat
    13441 aaaggagaaa tatgatgagt caacactctg ccggagcacg tttccgccaa gccgtgaaag
    13501 aatcgaatcc gcttgccgtc gccggttgcg tcaatgctta ttttgcacga ttggccaccc
    13561 aaagcggttt caaagccatc tatctgtccg gcggcggcgt ggcagcctgt tcttgcggta
    13621 tccctgattt gggcattacc acaatggaag atgtgctgat cgacgcacga cgcattacgg
    13681 acaacgtgga tacgcctctg ctggtggaca tcgatgtggg ttggggcggt gcattcaata
    13741 ttgcccgtac cattcgcaac tttgaacgcg ccggtgttgc agcggttcac atcgaagatc
    13801 aggtagcgca aaaacgctgc ggccaccgtc cgaacaaagc cattgtatct aaagatgaaa
    13861 tggtcgaccg tatcaaagct gccgtagatg cgcgcgttga tgagaacttc gtgattatgg
    13921 cgcgtaccga tgcgctggcg gtagaaggtt tggatgccgc tatcgaacgc gcccaagctt
    13981 gtgtcgaagc cggtgcggac atgattttcc ctgaagccat gaccgatttg aacatgtacc
    14041 gccaatttgc agatgcggtg aaagtgcccg tgttggcgaa cattaccgag tttggttcca
    14101 ctccgcttta tacccaaagc gagctggctg aaaacggcgt gtcgctggtg ctgtatccgc
    14161 tgtcatcgtt ccgtgcagca agcaaagccg ctctgaatgt ttacgaagcg attatgcgcg
    14221 atggcactca ggcggcggtg gtggacagta tgcaaacccg tgccgagctg tacgagcatc
    14281 tgaactatca tgccttcgag caaaaactgg ataaattgtt tcaaaaatga tttaccgctt
    14341 tcagactgcc tttcaacaaa tccgcatcgg tcgtctgaaa acccgaaacc cataaaaaca
    14401 caaaggagaa ataccatgac tgaaactact caaaccccga ccctcaaacc taaaaaatcc
    14461 gttgcgcttt ctggcgttgc ggccggtaat accgctttgt gtaccgttgg ccgtaccggc
    14521 aacgatttga gctatcgcgg ttacgacatt ctggatttgg cacaaaaatg cgagtttgaa
    14581 gaagtcgccc acctgctgat tcacggccat ctgcccaaca aattcgagct ggccgcttat
    14641 aaaaccaagc tcaaatccat gcgcggcctg cctatccgtg tgattaaagt tttggaaagc
    14701 ctgcctgcac atacccatcc gatggacgta atgcgtaccg gcgtatccat gctgggctgc
    14761 gttcatcctg aacgtgaaag ccatccggaa agtgaagcgc gcgacatcgc cgacaaactg
    14821 atcgccagcc tcggcagcat cctcttgtac tggtatcaat attcgcacaa cggcaaacgc
    14881 attgaggttg aaagcgacga agagaccatc ggcggtcatt tcctgcaact gttgcacggc
    14941 aaacgcccaa gcgaatcaca catcaaagcc atgcacgttt cactgattct gtatgccgaa
    15001 cacgagttca acgcttctac ctttaccgcc cgcgtgatcg ccggtacagg ctctgatatg
    15061 tactccagca ttaccggagc aatcggcgcg ttgaaaggtc cgaaacacgg cggcgcgaac
    15121 gaagtggctt acgatattca aaaacgctac cgcaatgccg acgaagctga agccgacatc
    15181 cgcgaacgca tcggccgcaa agaaatcgtg atcggtttcg gtcatccggt gtacaccatt
    15241 tccgaccctc gcaacgttgt cattaaagaa gtggcacgcg gtttgagcaa agaaaccggc
    15301 gatatgcgcc tctttgacat tgccgaacgt ttggaaagcg tgatgtggga agagaaaaaa
    15361 atgttcccga atctggactg gttctctgcc gtttcctacc aaaaattggg cgtaccgacc
    15421 gctatgttca caccgctgtt cgtaatttcc cgtacaaccg gttggagcgc acacgttctt
    15481 gagcaacgca aagacggcaa aatcatccgt ccgagcgcaa actacacagg ccctgaagat
    15541 ttggcgtttg tggagattga agaacgataa ttgaagaatg caatagcagt ttgttcttta
    15601 atttcggtat gcaaagctga ggatttcaga cgaccttgcc tgattggaaa ggttgtctga
    15661 aataagttta aaaatttaac ctaatagaag aagataatcc tgtattggtg caagtaacag
    15721 gataagaaac atggaagatt tatacataat actcgctttg ggtttggttg cgatgattgc
    15781 cggatttatc gatgcgattg cgggtggggg tggtttgatt acgctgcctg cactcttgtt
    15841 ggcaggtatt cctcccgtgt cggcaattgc caccaacaag ctgcaagcag ccgctgctac
    15901 gttttcggct acggtttctt ttgcacgcaa aggtttgatt gattggaaga aaggtctccc
    15961 gattgcggca gcatcgtttg caggcggcgt ggtcggtgca ttatcggtca gcttggtttc
    16021 caaagatatt ctgctggcgg tcgtgccggt tttgttgata tttgtcgcgc tgtattttgt
    16081 gttttcgccc aagctcgacg gcagtaagga aggcaaagcc agaatgtctt tttttctgtt
    16141 cggtctgacg gttgcaccac ttttgggttt ttacgacggt gtgttcggac cgggtgtcgg
    16201 ctcgtttttt ctgattgcct ttattgtttt gctcggctgc aagctgttga acgcgatgtc
    16261 ttacaccaaa ttggcgaacg ttgcctgcaa tcttggttcg ctatcggtat tcctgctgca
    16321 cggttcgatt attttcccga ttgcggcaac gatggcggtc ggtgcgtttg tcggtgcgaa
    16381 tttaggtgcg agatttgccg tccgcttcgg ttcgaagctg attaagccgc tgctgattgt
    16441 catcagcatt tcgatggctg tgaaattgtt gacagacgag agaaatccgc tgtatcagat
    16501 gattatttcg atgttttaaa ccctttcaga cgaccccttc aaaacgtcgg ctgaaacctc
    16561 aaaccacaag aaaaacagat ccccaggaga accgacatgg ctgccaacca acgttaccgc
    16621 aaaccgctgc ccggtacgga tttggaatac tacgacgcgc gtgcggcgtg tgaggacatc
    16681 aagcccggct cttacgacaa gctgccttac acgagccgca ttttggcgga gaatttggtc
    16741 aaccgcgcgg acaaagtcga tttgccgacg ctgcaaagct ggctgggtca gctgattgag
    16801 ggaaaacagg aaatcgactt tccttggtat ccggcgcggg tggtgtgcca cgatattctg
    16861 gggcagaccg cgttggtgga tttggcaggt ctgcgcgatg cgattgccga aaaaggcggc
    16921 gatcctgcca aagtgaatcc ggtggtgcaa acccagctca ttgtcgacca ctctttggcc
    16981 gttgaatgcg gcggttacga tcctgatgcc ttccgcaaaa accgcgaaat cgaagacaga
    17041 cgtaacgaag accgtttcca ctttatcaac tggacaaaaa ccgcatttga aaatgtggac
    17101 gtgattccgg cgggcaacgg catcatgcac caaatcaatc tggaaaaaat gtcgccggtt
    17161 gttcaagtta aaaacggcgt ggcgttcccc gatacctgcg tcggcacgga ttcgcacacg
    17221 ccgcacgtcg atgcgctggg cgtgatttcc gtgggtgtgg gcggattgga agcggaaacc
    17281 gtaatgctgg gacgcgcgtc catgatgcgc ctgcccgata ttgtcggtgt ggagctgacc
    17341 ggcaaacggc aggcgggcat tacggcgacg gatattgtgt tggcgctgac cgagtttctg
    17401 cgcaaagagc gcgtggtcgg ggcgtttgtc gaattcttcg gcgagggcgc gagaagcctg
    17461 tctatcggcg accgcgcgac catttccaac atgacgccgg agttcggcgc gactgccgcg
    17521 atgttcgcta ttgatgagca aaccattgat tatttgaaac tgaccggacg cgacgacgcg
    17581 caggtgaaat tggtggaaac ctacgccaaa accgcaggct tatgggcaga tgccttgaaa
    17641 accgccgttt atcctcgcgt tttgaaattt gatttgagca gcgtaacgcg caatatggca
    17701 ggtccaagta acccgcatgc ccgttttgcg accgccgatt tggcggcgaa agggctggcg
    17761 aagccttacg aagagccttc ggacggacaa atgcccgacg gcgcggtcat catcgccgcg
    17821 attaccagtt gcaccaacac ttccaacccg cgcaacgtcg ttgccgccgc gctgttggca
    17881 cgcaatgcca accgcctcgg cttgaaacgc aaaccttggg tgaaatcttc atttgccccg
    17941 ggttcaaaag tagccgaaat ctatttgaaa gaagcaggcc tgctgcccga aatggaaaaa
    18001 ctcggcttcg gtatcgtcgc cttcgcctgc accacctgta acggcatgag tggcgcgctg
    18061 gatccgaaaa cccagaaaga aatcatcgac cgcgatttgt acgccaccgc cgtactgtca
    18121 ggcaaccgca acttcgacgg ccgtgtccat ccgtatgcga aacaggcttt cctcgcttcg
    18181 cctccgttgg tcgttgccta cgcgctggca ggcagtatcc gtttcgatat tgaaaacgac
    18241 gtactcggcg ttgcagacgg caaggaaatc cgcctgaaag acatttggcc tgccgatgaa
    18301 gaaatcgatg ccgtcgttgc cgaatatgtg aaaccgcagc agttccgcga tgtgtatgta
    18361 ccgatgttcg acaccggcac agcgcaaaaa gcacctagtc cgctgtacga ctggcgtccg
    18421 atgtccacct atatccgccg cccaccttac tgggaaggcg cactggcagg ggaacgcaca
    18481 ttaagaggta tgcgtccgct ggcgattttg cccgacaaca tcaccaccga ccacctctcg
    18541 ccgtccaatg cgattttggc cgtcagtgcc gcaggcgagt atttggcgaa aatgggtttg
    18601 cctgaagaag acttcaactc ttacgcaacc caccgcggcg accacttgac cgcccaacgc
    18661 gctaccttcg ccaatccgaa actgtttaac gaaatggtga aaaacgaaga cggcagcgtg
    18721 cgccaaggct cgttcgcccg cgtcgaaccc gaaggcgaaa ccatgcgcat gtgggaagcc
    18781 atcgaaacct atatgaaccg caaacagccg ctcatcatca ttgccggtgc ggactatggt
    18841 caaggctcaa gccgcgactg ggctgcaaaa ggcgtacgcc tcgccggcgt agaagcgatt
    18901 gttgccgaag gcttcgagcg tatccaccgc accaacctta tcggcatggg cgtgttgccg
    18961 ctgcagttca aacccgacac caaccgccat accctgcaac tggacggtac ggaaacctac
    19021 gacgtggtcg gcgaacgcac accgcgctgc gacctgaccc tcgtgattca ccgtaaaaac
    19081 ggagaaaccg tcgaagttcc cgttacctgc cgcctcgata ctgcagaaga agtattggta
    19141 tatgaagccg gcggcgtgtt gcaacggttt gcacaggatt ttttggaagg gaacgcggct
    19201 tagaggtcgt ctgaaaagca agacgtagcg tgggtcgggt tcaacatttt gctcattcac
    19261 gtaattctcg atatggcagg catctactgt aaatcgtcat tcccgcgcag gcgggaatcc
    19321 agaaagtgga attgaggaaa cctttttatc cgatgagttt ctgtgcggat aaatctggat
    19381 tcccgcctgc gcgggaatga cggggtttaa taatctgccg tatcacaaca cagtagccgt
    19441 agattggggc gaaccccgac agtttgcgga atcaaacggc tttggtcgga gtggcagcct
    19501 aatccactat aaaaatcgtg ggcagagccc acgctacata aggagaatct agaaatgccg
    19561 caaattaaaa ttcccgccgt ttactaccgt ggcggtacat caaaaggcgt gtttttcaaa
    19621 cgttccgacc tgcccgaggc ggcgcgggaa gcgggaagcg cacgcgacaa aatcctcttg
    19681 cgcgtactcg gcagcccgga tccctacggc aagcagatag acggtttggg caacgccagc
    19741 tcgtccacca gcaaggcggt gattttggac aagtccgaac gcgccgatca cgatgtcgat
    19801 taccttttcg ggcaagtttc catcgacaaa ccttttgtcg attggagtgg caactgcggc
    19861 aacctcaccg ctgccgtggg cgcattctcc atcgaacagg gcttggtcga taaaggcaag
    19921 attccttcag acggcatctg cacggtcaaa atctggcaga aaaacatcgg caaaaccatt
    19981 attgcccatg taccgatgca aaacggcgca gttttggaaa caggcgattt tgagctcgac
    20041 ggcgtaacgt tcccggcagc cgaagtacaa atcgaatttc ttgatccagc cgacggcgaa
    20101 ggcagtatgt tcccaaccgg caatttggtc gatgaaattg atgtgccgaa tataggccgt
    20161 ttgaaagcca cgctcatcaa cgcgggcatt ccgaccgttt tcctgaatgc cgccgacttg
    20221 ggctacacgg gcaaagagtt gcaagacgac atcaacaacg atgccgcagc tttggaaaaa
    20281 ttcgagaaaa tccgcgctta cggtgcgctg aaaatgggtc taatcagcga cgtatccgaa
    20341 gctgccgccc gcgcgcacac gccgaaagtc gccttcgtcg cgcccgccgc cgattacacc
    20401 gcctccagtg gcaaaaccgt gaatgccgcc gacatcgatt tgctggtacg cgccctgagc
    20461 atgggcaaat tgcaccacgc gatgatgggt accgcctctg ttgccattgc gaccgccgcc
    20521 gccgtgcccg gtacgctggt caaccttgcc gcaggcggcg gaacgcgtaa agaagtgcgc
    20581 ttcgggcatc cttccggcac attgcgcgtc ggtgcagccg ccgaatgtca ggacggacaa
    20641 tggacggcca ccaaagcggt tatgagccgc agcgcacgcg tgatgatgga aggttgggtc
    20701 agggtgccgg aagattgttt ttaaattgac gtagcatggg tttgcccgcg agccataaac
    20761 aggtcgtctg aaaaacaagt aaacatcaaa tcactgacca ttcctttccc ttgccctgtg
    20821 gcggaaggcg gcaaatcaca aggaagaaca cggaaacccc gataaaagac agcttcccgt
    20881 attaccgtca ttcccgcgca ggcgggaatc tagacctgtc aatatggagg attggtaggg
    20941 gaaaacaggt ttcgtgagtt ctacattctg gattcccgcc acagcctgtc ctcgcgtagg
    21001 cggggacgga ataacgatag aaaatgcggc atacgctttg cccaaagagg ccgtctgaaa
    21061 caccttgcgc ctgatgtctg cctttttcag acgaccccac accaaaaaaa caaccacaaa
    21121 ctacaaggag aaacatcatg tccgaccaac tcatcctcgt tctgaactgc ggcagttcat
    21181 cgctcaaagg cgccgttatc gaccgaaaaa gcggcagcgt cgtcctaagc tgcctcggcg
    21241 aacgcctgac cacgcccgaa gccgtcatta cgttcaacaa agacggcaac aaacgccaag
    21301 ttcccctgag cggccgaaat tgccacgccg gcgcggtggg tatgcttttg aacgaactgg
    21361 aaaaacacgg tctgcacgac cgcatcaaag ccatcggcca ccgcatcgcc cacggcggcg
    21421 aaaaatacag cgagtctgtt ttgatcgacc aggccgtaat ggacgaactc aatgcctgca
    21481 ttccgcttgc gccgctgcac aaccccgcca acatcagcgg catccttgcc gcacaggaac
    21541 atttccccgg tctgcccaat gtcggcgtga tggatacttc gttccaccaa accatgccgg
    21601 agcgtgccta cacttatgcc gtgccgcgcg agttgcgtaa aaaatacgct ttccgccgct
    21661 acggtttcca cggcaccagt atgcgttacg ttgcccctga agccgcacgc atcttgggca
    21721 aacctctgga agacatccgc atgattattg cccacttagg caacggcgca tccattaccg
    21781 ccatcaaaaa cggcaaatcc gtcgatacca gtatgggttt cacgccgatc gaaggtttgg
    21841 taatgggtac acgttgcggc gacatcgatc cgggcgtata cagctatctg acttcccacg
    21901 ccgggatgga tgttgcccaa gtggatgaaa tgctgaacaa aaaatcaggt ttgctcggta
    21961 tttccgaact ttccaacgac tgccgcaccc tcgaaatcgc cgccgacgaa ggccacgaag
    22021 gcgcgcgcct cgccctcgaa gtcatgacct accgcctcgc caaatacatc gcttcgatgg
    22081 ctgtgggctg cggcggcgtt gacgcactcg tgttcaccgg cggtatcggc gaaaactcgc
    22141 gtaatatccg tgccaaaacc gtttcctatc ttgatttctt gggtctgcac atcgacacca
    22201 aagccaatat ggaaaaacgc tacggcaatt cgggcattat cagcccgacc gattcttctc
    22261 cggctgtttt ggttgtcccg accaatgaag aactgatgat tgcctgcgac actgccgaac
    22321 ttgccggcat cttgtagcca aaaaagggac gagtccgcaa aaatgccgtc tgaaacccca
    22381 aacgcccgat taggctgatg aggattttag acggcattgt tcattttttt gttatcttgc
    22441 atttttgtgc ggacggtgga atttcatcct gtaaacataa atatttgtcg gaaaacagaa
    22501 accctccgcc gccatttcta cgaaagcagg aaaccagcaa cgcaaagcga cagggatttg
    22561 ttggaaatga ccgaaaccga acgaaccgga ttcccgcctg cgcgggaatg acgggatttt
    22621 ctgtttttgt ggaaatgacg ggattttgaa tttcgggcgt acaatacgga aaacatgacg
    22681 ataaggaaac aaaccatggc acagtttttc gctattcatc ccgacaatcc ccaagaacgc
    22741 ctcatcaagc aggcggttga aatcgtcaat aaaggcggcg tggtcgttta tccgaccgat
    22801 tcctgttatg ccttgggctg caaactcggc gataaggcgg cgatggaacg catactctcc
    22861 atccgcaaaa tcgatttgaa acaccacctg accctgatgt gcgcagattt gagcgagttg
    22921 ggcacatacg ccaaagtcga caacgtacag tttcgtcagc ttaaagccgc cacacccggg
    22981 ccttatactt ttattttaca ggcgacgaag gatgtgccgg cgcgcacgct gcacccgaaa
    23041 cgcaaaacca tcgggctgcg tattcccgat aatgccattg cacaagccct gctgggggaa
    23101 ttgggcgagc cgcttttaag ctgcaccctg atgctgcccg aagacggcga accattgacc
    23161 gatccttatg aaatccgcga gcgtttggaa cacgccgtcg atttggtgat tgacggcggc
    23221 tggtgcggaa ccgagccgac caccgtcgtc gatatgaccg acggcacgga attggtgcgc
    23281 caaggttgcg gcgatacggc ggtgttcggt ttgtagggaa accgatgccg tctgaagcat
    23341 cggctgttca gacggcattg cgcgccttgc cggcggcagt ccgaaatgcc ggcgcgtatc
    23401 gcgctcggtc ggaatatccg tttgaaacgg cattttgatg cattactgca ccgcaatcgg
    23461 aattctcggt tcgtagagca ggtcgtaggt cggcttgttg agcaggtctt ggagcgtgaa
    23521 accgtccaga tacgtgaaaa acgacttcat cgcgccgccg agtatgcccg tcagccggca
    23581 ggacggtgta atcaggcatt cgttgttctc gcccatgcac tcgaccagct gcatcggttc
    23641 gaggtggcgg acaaccgagc cgatgttgat gcggtcgggc ggtgcggcaa gccgcagacc
    23701 gccgcctttt ccgcgcacac tgtggaggaa gccgcctttg accagcgcgg taacgacctt
    23761 catcagatgg cttttggaaa tgccgtaggt tacggcgatg gtactgatgt tgaccagcgc
    23821 atcgtcgttg atggcagtgt agataaggac gcgcagcccg tagtccgtat gttgtgtcaa
    23881 atacatgatt ttctcggtat ggattgttat tcttatcggt acggtttaag gttcacggac
    23941 aataccttaa tggttgaaac cctgtccgtc ggggcggtag aatgcagcct gtctgcggcg
    24001 gtatgccgtc tgaaacatcc gcgctaccgt ttgagaattt gttattgtaa ctcaaaatca
    24061 tgaaaccgtt gaaacgacat cccgccctta tcgggctttc gtgtgaccac caccattcgc
    24121 tttccctgtg cgtgcgtctg ttgcggacgc cggaagaaag gcatcgggac gaactcgaac
    24181 cgcatttttc cgaattggaa acccattttc gcgaagaaga aaccaagttt gccccaattt
    24241 ggcagaatgt cgcccccgaa ttgaaacaac gtttcgagaa agaccacgcc cgactgcggc
    24301 agatgatggc aagccccgaa tacggtaacg cggcgtggaa taccgctttt gccacaaccc
    24361 tgcgcgacca cgcgcgcttt gaagaacgcg agctgtttcc cgccgccgaa ccgtttttgc
    24421 cggcatgatt ccgttttgcg gtaaatatat taatgataaa caaggaacac acatgaaatt
    24481 taccaagcac cccgtctggg caatggcgtt ccgcccattt tattcgctgg cggctctgta
    24541 cggcgcattg tccgtattgc tgtggggttt cggctacacg ggaacgcacg agctgtccgg
    24601 tttctattgg cacgcgcatg agatgatttg gggttatgcc ggactggtcg tcatcgcctt
    24661 cctgctgacc gccgtcgcca cttggacggg gcagccgccc acgcggggcg gcgttctggt
    24721 cggcttgact atcttttggc tggctgcgcg gattgccgcc tttatcccgg gttggggtgc
    24781 gtcggcaagc ggcatactcg gtacgctgtt tttctggtac ggcgcggtgt gcatggcttt
    24841 gcccgttatc cgttcgcaga atcaacgcaa ctatgttgcc gtgttcgcgc tgttcgtctt
    24901 gggcggcacg catgcggcgt tccacgtcca gctgcacaac ggcaacctag gcggactctt
    24961 gagcggattg cagtcgggct tggtgatggt gtcgggtttt atcggtctga ttggtacgcg
    25021 gattatttcg ttttttacgt ccaaacgctt gaatgtgccg cagattccca gtccgaaatg
    25081 ggtggcgcag gcttcgctgt ggctgcccat gctgactgcc atgctgatgg cgcacggtgt
    25141 gttggcttgg ctgtctgccg tttttgcctt tgcggcaggt gtgattttta ccgtgcaggt
    25201 gtaccgctgg tggtataaac ccgtgttgaa agagccgatg ctgtggattc tgtttgccgg
    25261 ctatctgttt accggattgg ggctgattgc ggtcggcgcg tcttatttca aacccgcttt
    25321 cctcaatctg ggtgtgcatc tgatcggggt cggcggtatc ggcgtgctga ctttgggcat
    25381 gatggcgcgt accgcgcttg gtcatacggg caatccgatt tatccgccgc ccaaagccgt
    25441 tcccgttgcg ttttggctga tgatggcggc aaccgccgtc cgtatggttg ccgtattttc
    25501 ttccggcact gcctacacgc acagcatccg cacctcttcg gttttgtttg cactcgcgct
    25561 tttggtgtat gcgtggaagt atattccttg gctgattcgt ccgcgttcgg acggcaggcc
    25621 cggttgagac aaaccgccgc agatttcggg tctgggcggt ttgcttttca gacggcaggg
    25681 cggtcagttg ccgtccagcc agcggtcgcg tgtggttttg gcttcttcaa aatagcggta
    25741 cagggcttcg cggtcgtcgg tggtcaggat gtttgccaaa acgtccaact gtttgcccaa
    25801 gccttgaacc agttgcagca ggctgtcttt gttggcaagg cagatgtccg cccacacggc
    25861 gggatgaccg gaggcgatgc gggtgaagtc ccgaaagccc gtggcggcga atttcagata
    25921 ttcctgtccg tcggggtggt cgagaatctg gtggacatag gcgaaggcgg tcaggtgggg
    25981 catatgggag acggcggcga aaaccgcgtc gtggcgttgc gcgtccatcg tataaatttc
    26041 cgcaccgacc gcgtgccaca ggttttctac caaggcaatg ccgtctgaat gttcgccgcc
    26101 gtgtggcgtg atgatgagtt ttctgtggcg gaacagcccg aactgcgcgg cttgcgcacc
    26161 gcttctgtcc gaaccggcaa ttgggtgggc ggcgatgcag tggtgcaggc ggtcgggcag
    26221 acagcggcgg aaggcttcga tgaccgaaga tttggtgctg ccgacatcgg aaatccaagt
    26281 gtgttccggc aaaacggggc gcagcgcggt caaaatggcg ggaacggtgg cgacgggcgt
    26341 ggcaatcagt accaagtccg caccgccgat gctgtccgcg tcgatggcaa cggaagcctg
    26401 gtcaatcacg ccgcgttcca atgcacgttc gaggttgtcg cggtcggtgt cgataccggt
    26461 aacggtgcgg acgagtccct gccttttgag gtcgagaacg aacgaaccgc cgatcagccc
    26521 tacaccgatg agggcaatat ggttcaaaat gggcatttgt gtaaacggtt ttcgcaaagt
    26581 accgtcatgg tagcctatcg gcggaatatg ccgcaaggtc ggcaggaaaa aggagaagaa
    26641 atggacaaaa tcagagttgc cgccgtgcag atggtgtcgg gcgtgtcgcc ggaaaccaac
    26701 gtcgccgcca tgaaacgcct ggtcgcacgg gcggcggagc agggtgcgga ttgggtgctg
    26761 ctgcccgaat attgggtgct gatgggcgca aacgataccg acaaactcgc gcttgccgag
    26821 cctttgggcg gcggacgctt tcagacggca ttgagcgaaa cggcgaaaga atgcggcgtg
    26881 gtgctgttcg gcgggactgt gccgctgcaa agctgcgagg cgggtaaagt gatgaatacg
    26941 ctgttggtgt acggacggga cggcgtaagg acggggctgt accacaaaat gcacctcttc
    27001 ggtttttccg gtttgggcga acgctatgcc gaagccgata ccatccgcgc gggcggggat
    27061 gtgccgcact tgtcggcaga aggcgtgccg gtggcggcgg gcatttgtta cgatgtccgc
    27121 tttcccgaat ttttccgacg ccagttgccg tttgacgtat tgatgctgcc cgctgcgttt
    27181 acgcacacga cgggcaaggc gcattgggag ctgctgctgc gcgcgcgtgc cgtcgaaaac
    27241 caatgttacg tcgtggcggc ggcacagggc ggtttgcacg aaaacggacg gcgcacgttc
    27301 ggacacagca tgattgtcga tccgtggggc gacgtgttgg acgtattgcc cgagggcgaa
    27361 ggcgttgtta cggcagacat cgatgccaac cgcctgaaca gcgtccgcaa ccgcctgccc
    27421 gccttgaaat accgggtttt g
//
LOCUS       DAWHXS010000012        27240 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWHXS010000012 DAWHXS010000000
VERSION     DAWHXS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.012"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..135
                     /locus_tag="WIE22_000413"
     CDS             <1..135
                     /locus_tag="WIE22_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4126556.1"
                     /translation="NPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNADFSIEYD
                     "
     gene            207..368
                     /locus_tag="WIE22_000414"
     CDS             207..368
                     /locus_tag="WIE22_000414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126557.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            418..702
                     /locus_tag="WIE22_000415"
     CDS             418..702
                     /locus_tag="WIE22_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126558.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWAIGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(827..1543)
                     /gene="trmB"
                     /locus_tag="WIE22_000416"
     CDS             complement(827..1543)
                     /gene="trmB"
                     /locus_tag="WIE22_000416"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ4126559.1"
                     /translation="MTDTAENQTPNERQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1665..3068)
                     /locus_tag="WIE22_000417"
     CDS             complement(1665..3068)
                     /locus_tag="WIE22_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ4126560.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLAAAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDIYRYGLGMTSDKEKALHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            complement(3341..5227)
                     /gene="uvrC"
                     /locus_tag="WIE22_000418"
     CDS             complement(3341..5227)
                     /gene="uvrC"
                     /locus_tag="WIE22_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ4126561.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     ILQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(5282..7444)
                     /locus_tag="WIE22_000419"
     CDS             complement(5282..7444)
                     /locus_tag="WIE22_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ4126562.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRDGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIATWLIKGDWTLALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGKPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLEIPTAQNAQTIVGAGITAEVKGAGLVKAGKAEF
                     AELTLPKFSDGVWEIASVVAVSVNGKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            7583..8533
                     /gene="trxB"
                     /locus_tag="WIE22_000420"
     CDS             7583..8533
                     /gene="trxB"
                     /locus_tag="WIE22_000420"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ4126563.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8689..9057
                     /gene="rpsF"
                     /locus_tag="WIE22_000421"
     CDS             8689..9057
                     /gene="rpsF"
                     /locus_tag="WIE22_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ4126564.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            9058..9360
                     /gene="priB"
                     /locus_tag="WIE22_000422"
     CDS             9058..9360
                     /gene="priB"
                     /locus_tag="WIE22_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ4126565.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            9367..9597
                     /locus_tag="WIE22_000423"
     CDS             9367..9597
                     /locus_tag="WIE22_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ4126566.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            9614..10066
                     /locus_tag="WIE22_000424"
     CDS             9614..10066
                     /locus_tag="WIE22_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ4126567.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            10080..10187
                     /locus_tag="WIE22_000425"
     CDS             10080..10187
                     /locus_tag="WIE22_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ4126568.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            10762..10917
                     /locus_tag="WIE22_000426"
     CDS             10762..10917
                     /locus_tag="WIE22_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126569.1"
                     /translation="MYTGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(11033..11839)
                     /locus_tag="WIE22_000427"
     CDS             complement(11033..11839)
                     /locus_tag="WIE22_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ4126570.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(11840..12586)
                     /gene="pssA"
                     /locus_tag="WIE22_000428"
     CDS             complement(11840..12586)
                     /gene="pssA"
                     /locus_tag="WIE22_000428"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ4126571.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(12641..13063)
                     /locus_tag="WIE22_000429"
                     /pseudo
     CDS             complement(12641..13063)
                     /locus_tag="WIE22_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13169..13651
                     /locus_tag="WIE22_000430"
     CDS             13169..13651
                     /locus_tag="WIE22_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ4126572.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(13703..14914)
                     /locus_tag="WIE22_000431"
     CDS             complement(13703..14914)
                     /locus_tag="WIE22_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ4126573.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            15137..17575
                     /locus_tag="WIE22_000432"
     CDS             15137..17575
                     /locus_tag="WIE22_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ4126574.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            17784..19094
                     /locus_tag="WIE22_000433"
     CDS             17784..19094
                     /locus_tag="WIE22_000433"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ4126575.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            19190..19804
                     /gene="clpP"
                     /locus_tag="WIE22_000434"
     CDS             19190..19804
                     /gene="clpP"
                     /locus_tag="WIE22_000434"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ4126576.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(19861..21126)
                     /gene="ispG"
                     /locus_tag="WIE22_000435"
                     /gene_synonym="gcpE"
     CDS             complement(19861..21126)
                     /gene="ispG"
                     /locus_tag="WIE22_000435"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ4126577.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSVWRTAYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(21142..21903)
                     /gene="pilW"
                     /locus_tag="WIE22_000436"
     CDS             complement(21142..21903)
                     /gene="pilW"
                     /locus_tag="WIE22_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ4126578.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQKSFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(21906..23000)
                     /gene="rlmN"
                     /locus_tag="WIE22_000437"
     CDS             complement(21906..23000)
                     /gene="rlmN"
                     /locus_tag="WIE22_000437"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ4126579.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(23144..23569)
                     /gene="ndk"
                     /locus_tag="WIE22_000438"
     CDS             complement(23144..23569)
                     /gene="ndk"
                     /locus_tag="WIE22_000438"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ4126580.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(23628..24779)
                     /locus_tag="WIE22_000439"
     CDS             complement(23628..24779)
                     /locus_tag="WIE22_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ4126581.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKDSGG"
     gene            complement(25170..25997)
                     /gene="fghA"
                     /locus_tag="WIE22_000440"
     CDS             complement(25170..25997)
                     /gene="fghA"
                     /locus_tag="WIE22_000440"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ4126582.1"
                     /translation="MKLIEQHQIFGGLQQVWAHDAQTLQCEMKFAVYLPNNSENQPLG
                     VIYWLSGLTCTEQNFITKSGFQLYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWATNYQMYDYILNELPDLVEANFSTNGKRSIMGHSMGGHGALVLALRNRK
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(26006..27115)
                     /locus_tag="WIE22_000441"
     CDS             complement(26006..27115)
                     /locus_tag="WIE22_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ4126583.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
BASE COUNT         6474 a         6614 c         7350 g         6802 t
ORIGIN      
        1 aatccggtca aacatggcta tgtaggacaa atttccgatt ggcggttttc tacgtttcac
       61 cgttatgtca aacagggtat ttatccgcat aattggggtg gcggcaatgc ggacttttct
      121 attgaatacg attaaagtaa tgtcggattc aagaatccga cctacggaaa actaaagatt
      181 ttatacgggc tacggcttgc tggcttatgc gttggcttaa tttgaaaaac agcacatcgg
      241 ttcataatac acaaggtatt agaacaaaag aagcagttga acagggattg cgtggtaata
      301 atagacctga aaaaggaata ggtaatagat ggaattatga tggatttata aaggaattag
      361 cgaagtaaca ggtattccaa atatttctga taaatataaa gaacaaggag taaaaaaatg
      421 aaagtaatca ctattacatt gtttactagc tgcatactat ctggatgcta tgggcttgaa
      481 tatggaatga gtggcggcat tcctcatgat gactgggcaa taggtagcca tgttagtcaa
      541 gccaaagcag atgctgcgtg gaaagaatgt tctgacaaat atatgagaaa tggtggttca
      601 ggattagtag aacaatgtat gaatgataaa ggattaatac gtttatctga aatacgtcgt
      661 aggcaaaaga gagcagaaac acaaagagca gtaattaagt aacaataatg aaatactact
      721 gttcccgaac aaagcggcat ttacgcaggt gatgacggct atattttgaa aaatgccgtc
      781 tgtaagcaaa cgtgcttcag acggcatttt tttatccggc ggtttgttat ccgatccgtt
      841 tgaataccaa gtcccaaacg ccgtgtccga ggcgtttgcc gcgcgcttcg aatttggttt
      901 cggggcggta gtccggggtg ggggcgtagt ctgccgccgt attttgcagg ctgtcgaagc
      961 tgctgaggac ttcaagcatc tgctgcgcat attcttccca gtctgtcgcc aagtggatat
     1021 agccgccggt tttgagtttg ggcagtagtt tggcgatgaa gggggcttgt atcaggcggc
     1081 gtttgtggtg gcgttttttg tgccaggggt cggggaagaa tatgtggatg ccatcgagcg
     1141 agccgtcttg cagcatattt tcgacaactt ctacggcatc gtgccgcatc acgcggatgt
     1201 tttccaaatg gttttcgtct atgagtttga gcaggttgcc tacgccggga ccgtgtacgt
     1261 cgatggcgag aaagtcggtt tcgggcagcc tgcgggcaat ttctgcggtt gccgtcccca
     1321 taccgaagcc tatttcgagg attttgggtc ggctgcttcc gaaacgggca tcaagatcgg
     1381 ccggtgttgc ttggtagtcg atgccgaagc tgtcccataa ggtatcgatg gcgcgttgct
     1441 gcgcggcggt catatggctt tggcggagga cgaagctgcg gatgctgcgg gggtgtcctg
     1501 cttgacgctc gtttggtgtt tggttttcgg cggtgtctgt cataatgctt tcatgcgctt
     1561 gtgcaatcag gatgccgtct gaatgtgaaa tgtctgttcg gacggcggga agggaaaggg
     1621 gcgattatac ctgtttcgtt tcggcagggt aggtgtaacg ctgttcagac ggcattttgc
     1681 cactgttgca acagttcttg caggacgctt ttttgcccgt atccgttgtt tatggcttct
     1741 tgcaggtagc ggcaggcttt ttctttgtcc ggcccgacac cgtgtccgct gtaatggatg
     1801 caggcgaggt tgtagaacgc catactgtct ttttttgcgg cggctttttc aaaccatttg
     1861 cgtgcttcat tgtagtcggc ggtcataccc tgtccgaagt aatacatgct gcccagattg
     1921 gtttgggctt tgctgtgtcc gagttctgcg gcttctgtgt agagtttgag cgcggctgcg
     1981 tattcgggcg cgcattgtaa tccgtaatgc agggcttggg ctttttgata aagccgttct
     2041 gccctttgcc gcctgatggc ggaatcttta atgccgccat attggtcggg atggtttaac
     2101 gcgctgtcgg atatgagttt ctgataggcg gtaaggctgc cggcttcggc tgcctgccgg
     2161 taataatgca gggctttttc tttatcggaa gtcatgccca gtccgtagcg gtagatgtcg
     2221 cccagtatcc gcaaaccttc gggatggcgt tcggcggcgg ctgcttcggc gtgatgcagg
     2281 gcaagtttgt agtttctttc cggatgcttg ccttgcaggt agatgtcggc aagtgcggca
     2341 tgggcatcgg ggtcattttc ctgtgccgcc tgttgaaacc agtgaacggc ttcatcagga
     2401 gccgtaggta ggatgagctc ggcaagcggg gtgtaggcgg gaatgtatcc ttgttgtgcg
     2461 gcggcgcgca aatgattgac ggcctgtgcc gtgtcgactt tcgtcccttg tccgaaacgg
     2521 tattgcagcc cgagctgcca gtgtgccgcc gccaagcctt gtgccgccgc tttgcggtag
     2581 agcaggtgcg ccgtttcaaa atccggcggc gtgccgtacc ggcagtattg ggcaagcagg
     2641 tattgcccgt gggggttgtt gttttttgcg cttgtttttg cccatttcag ggcttcttca
     2701 aaatgtcggg acgcatacag aaggtacatc aggcggactt gtgcttcagg cagtccttcc
     2761 tctgccaacg gtctgtaaag cggtatcagg gtttccgggg gcgcgccttc caattcttgg
     2821 atatatgcca gattgtaacg cgcataggga tgcccctgtt cagcggcgat gcggagcatt
     2881 tccatacctt gttcgcggtc ttggtagggg ctgtccgcgt ccatcaggtg ttttgccaac
     2941 tcgaatgcgg cggcgggatg acctgccgag aacgcttctg tcagcagtcg gcggacttcg
     3001 gtaaagttat attgtttttg agacaggcat tctaaggcct gccgataggg taagtcggga
     3061 ttgttcatgt cagatcgttt tccgttttgt cgaatcatcg ttattgtgtt atgaagttag
     3121 gtttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     3181 tacgaaaccg attcacttgg tgcttgagca ccttagagaa tcgttctctt tgagctaagg
     3241 cgaggcaacg ccgtactggt ttaaatttaa tccactatat atcggtatac cgacctgctg
     3301 tatcatattt tttacagacg gtttttgctt gggcagcata ctaatgcaga tgctcgtaaa
     3361 tggtttccgc caatgccttg ctgatgcctt ccactttttc caagtcctcg cggctggcgg
     3421 caatcacgcc gcgcagaccg ccgaagcggg tgagcagggc ttggcggcgt ttgctgccta
     3481 cgccggggat gtcgcttaag gaggaggtaa cgcgggcttt gtcgcgtttt ttgcggtggc
     3541 cggtaatggc gaaacggtgc gattcatcgc gtacggtttg caatagatgc aaggccgggc
     3601 tgttgggcgg caggcggaag acttcgccgg taaaaggcag tatgagttct tccataccgg
     3661 ctttgcgctc cgggcctttg gcgataccga ccaaaggaat gtgcagcccg agttcttccc
     3721 ataccgatac ggcgatgccg atttgccctt tgccgccgtc aattaacacg gcatccggcc
     3781 atttgacggt ctcgccgttg gcttcggctt cctgcatttt gccgtaacgg cgcgtcaaca
     3841 cttcgcgcat ggcggcgtag tcgtcgccgg gtttggcggt cgtgatgttg tagcggcggt
     3901 attgcgaggg ctggatgttt tgctcatcgt acacgacgca ggaggcgatg gtggcttcgc
     3961 cttgggtgtg gctgatgtcg aagcattcga ggcggttgag gccgtctgaa tccataccga
     4021 ggattttggc gagttcgtcg atgcggtgtt gctggttgct ttgttgcagg cggcgttgtg
     4081 caatcgccat ttgtgcgttt tgttccgcca ttttcaacca aactttgcgt tcgcctatgg
     4141 ttttggttac gaactgcatt tgtttgccgt gttcgctctc tagggcttct ttcaatgcgt
     4201 cgggcatggg gaagttgctg atgatgatgt cgggtttgct tttgcccaaa taatgttggg
     4261 cgacgaaggc ttcggcgtaa tcttgtccgt ttggctcggg atcgtttttg gtgtcgggga
     4321 aaaagctttt atcgcctaca tgccgtccgc cgcgtatgct gacccagtgt acgcagacga
     4381 ggccgtctga aacggcgagc gcgagcaggt cgatgtcgtt ggggttgttc gggtttttgc
     4441 tgtcgatgaa ctggttgctc tgcatgatgc caagtgcttg aatttgatcg cggtaacgtg
     4501 cggcttcttc aaattgcaga ttcgcggcgg cagtttgcat tttgtgttgc agggtgcggg
     4561 tcagttcgtc ggttttgccg ttgagaaaag tggcggcttg acgcacgctg tcgcggtagt
     4621 cttcttcgct gatgtgtccg acgcagggcg cggtacagcg tttgatttga tacagcaggc
     4681 aagggcggtc acgatgctcg aatacgctgt cttcgcaggt acgcagcata aagacttttt
     4741 gcaggatttg gatgctgtcg cgcacagcgt tgctgttggg atacgggccg aagtattggt
     4801 tgggcttttt cagcgtgccg cggtaatacg ccatttgcgg atattgatgg ccgctgagca
     4861 tcaaataagg atagcttttg tcgtcgcgga aaaggatatt gtatttcggc gacaaggctt
     4921 tgatgaagtt gttttcgaga atcagggctt cggcttcgga acgcgtgatg gtggtttcga
     4981 tatgatgaac ctgttttacc atcaatgcga tgcgcggcga atggtcgttt ttttggaaat
     5041 agccggacac gcgccgcttg aggttgaccg ctttgccgac gtataagact ttgccgcctt
     5101 cgtcaaaaaa acggtacacg cccggcagct tgggcaggtt tttgaggaag agcgggatgt
     5161 cgaattgttc cggagaacgg ttttctgtat tcatatcaat tatttgaaaa acggcttcag
     5221 acggcatatc ggcagcggca gccttatccg ttcggaaaag ctgtcgggtg gttatatgcg
     5281 gtcaatcgat ttttacccgt ttcaggcgca aggcgttgct caacacggaa accgagcttg
     5341 ccgccattgc cgcgcctgcg atgacggggt ttaaaaagcc gagcgcggcg agcggaatgc
     5401 ccaaaatatt gtagaagaag gcgaaaaaca ggttttgctt gatgtttttc aacgtcgctc
     5461 gcgataccga tagcgcatcg gcgagctggt tgaccgaatg ctgcatcagt gtggcggatg
     5521 cggtgtgttc ggcaacgtct gcaccgcctt tcatggcgaa gctgacgttg gcggcggcga
     5581 gcgcgggcgc gtcgttgatg ccgtcgccga ccatcgccac ggttttgccg gcggctttga
     5641 gtttctgcac ttcggcggct ttgtcgcgcg gactcatatt accgaaggcg tgtgcgatgc
     5701 ccagttgttt ggcgacgtac tcgaccgtgc cttggttatc gccgctcata atatagacat
     5761 cgatattgtg ttttttcaga cggcctatgg cttcggcggt atcggctttc aacgcgtcgg
     5821 cgagtgcgaa tgcgccgata ggtttgccgt ttacagatac ggcaaccaca ctggcgattt
     5881 cccaaacgcc gtctgaaaac ttcggcaagg tcagttcggc aaattcggct ttgcctgctt
     5941 ttaccaaacc cgcgcctttt acttcggcgg taatgcccgc gccgacaatg gtttgggcat
     6001 tttgtgcggt gggaatctcc aaaccgcgcg cctgggcggc ggagacgatg gcacgggcga
     6061 gcggatgggc ggcgttttgt tcgacggcgg cggcgatgcg gtacaaagcg tcttcgtcaa
     6121 agccgctgtc gggaacacaa taaacggcgg caacctgcgg cttgccttcg gtcagcgtgc
     6181 cggttttgtc cagcacgacg gcatcaacgt gggcggcttc ttccattgct gccgcgtctt
     6241 taaaccaaat accgtgttta accgctttgc ccataccgac cataatcgca gcaggggttg
     6301 ccaaaccgag tgcacacggg caggcaatca ccaaaacggc gacggcgtgc atcaatgcga
     6361 gcgtccaatc gcccttaatc agccaagtag cgataaaagt caaaagtgcg atgcccacaa
     6421 cggcaggcac gaataccgcc gccgccttgt ccgccacacg cgcaatcggt gctttactgc
     6481 cttgcgcttc ggagagcgcg ttcatcatgt cgccgagcag ggtttggctg ccgagctgcg
     6541 cggcgcggta caccacgctg ccttcagtca tcagcgcacc cgccaatact ttgccgcctg
     6601 cctttttctc ttcgggattg gattcgccgg taagatggct ttcgtccgcc cagccgctgc
     6661 cgctttctat gatgccgtcg gcggcaatgc gttcgccgtg gttggcgcgg attaggtcgc
     6721 cgatttgcac ttggtcgatg ggcagttgtt tccattcgcc atcgcgttgc acgttgactt
     6781 gggtcggcgt gagtttgagc agcaaaccca agctgttcag gctggatttt ttggtgcggt
     6841 gttccaaaaa tttgcccagc gacacaaaac caatcaccat cacgcctgct tcaaaataca
     6901 catgcgccat gccgtgcgcc gcatgcggac tgaaaaacag catatagacg gaatacaggt
     6961 aaatcgagac cgtgccgatg gtaaccagca cgtccatatt cgccagcccg cctttaatgc
     7021 tcgcccacgc gcttttgtaa aatggcaccg ccagccaaag ctgcaccaca ctcgccaatg
     7081 caaactgcca caacggcgga atcatccaat cgtgtctgcc gatcatcatc cccgccatgc
     7141 cgataaggaa cgggacgttg atggtgaaca gcagccacag ccgccagccg atatggtgtt
     7201 ctgcttcggg ttgcggcaat gtatcttccg ttttttcctt cgcgccgtaa ccggttttct
     7261 caatgatttt ggcaatgtcg gctactgagg ttttgctgtc gtcaaacact acctgcgcct
     7321 cttcgctggc gaagtttacc cccgccgatt cgacaaaatc ttttttgttc aacacttttt
     7381 caatgcgcga agcgcaggcc tggcaggtca tgccttcgat ttggaaacgg attttttgtt
     7441 gcataagcgt gattcctgct ttgatattca gacggcagtt aaggctgcgg gatgccgtct
     7501 gagcgccggt atggtcgaaa tttctcaaac gcgccgataa ttcgagtaga atccgaaaac
     7561 ttgacacagc aaaggaaaaa caatgtccca acaccgcaaa ctgattattt tgggttccgg
     7621 ccccgccgga tacaccgccg ccgtctatgc cgcgcgtgcc aatttaaacc ccgtcattat
     7681 tacaggtatc gcgcaaggcg ggcaactgat gaccaccacc gaagtggaca actggcctgc
     7741 cgatgccgac ggcgtgcaag ggccggaatt gatggcgcgg tttctcgccc acgccgaacg
     7801 tttcggaacg gaaatcattt ttgaccaaat caacgccgtc gatctgcaaa aacgcccgtt
     7861 tacactcaaa ggcgatatgg gcgagtacac ttgcgatgcc ctgattgtcg caacaggcgc
     7921 gtccgccaaa tacctcggtt tgccgagtga ggaagcgttt gcaggaaaag gcgtttccgc
     7981 ttgtgccacc tgcgacggtt tcttctacaa aaaccaagat gttgccgtag ttggcggcgg
     8041 caatacggca gttgaagagg cactctacct tgccaatatc gccaaaaccg ttaccctgat
     8101 ccaccgccgc agcgagttcc gtgccgaaaa aatcatgatc gacaaactga tgaaacgcgt
     8161 ggaagagggc aaaatcatcc tcaagctgga aagcaacctg caagaagtac tgggcgacga
     8221 tcggggcgta aacggcgcat tattaaaaaa caacgacggt tctgagcaac aaattgccgt
     8281 cagcggcatt tttatcgcca tcgggcacaa gccgaatacc gatattttca aaggtcagtt
     8341 ggaaatggac gaagccggtt acctgaaaac caaaggcggc acggcggaca atgtcggcgc
     8401 aaccaatatc gaaggcgtat gggcggcagg cgacgtaaaa gaccatacct accgtcaggc
     8461 gattaccagc gcggcttccg gctgtcaggc ggctttggac gcggaacgct ggctgggcag
     8521 ccaaaacatt tgaatgccgt ctgaaaccga aattttcggc ggaatgcaaa tttttcgcac
     8581 caaatccttg attccgtcgg caaaccccaa tataatgcct gctctttgaa ccttgctttc
     8641 tgctttcgca tggcagaagg ctgtttttac catccataag gagataacat gcgtcattac
     8701 gagatcgtgt ttatcgttca tcctgatcaa agcgagcaag tgcctgctat ggttgaacgt
     8761 tacaaaacca tgattgccga agcaaacggt aagattcacc gtttggaaga ttggggtcgc
     8821 cgccaactgg cttacccgat taacaaaatc cataaagccc attacgtttt gatgaacatc
     8881 gaaaccactc ccgaagtggt tgaagagctg gaaaccgcat tccgcttcaa tgatgccgta
     8941 ttgcgccatc taaccatcaa aaccaaacac gccgttaccg aagcatcccc tatgttgggt
     9001 ggtgaaaagg ctaagaacct gttgagcggt gcgtctgaag aagcggtcgc ccaataattg
     9061 ggattcaata atcttgtttc gcttgccgcg ttaattgaaa aggttttccc tattcgatat
     9121 acgcctgccg gaatccctgt tttagatatt attttaaagc acaaatcgtg gcaggaggaa
     9181 aacgggcagc aatgccttgt ccaattggaa attccggcac ggattttagg caggcaggcg
     9241 gaagagtggc agtatcggca aggtgtatat gttcacgtcg aaggtttttt agctcaaaaa
     9301 agcagacgtt cccttatgcc gatgctcagg atacaaaata ttcaagaata taaaggttaa
     9361 acgacaatgg ctcgtcaatc attcaaacgt agaaaattct gccgtttcac ggctgaaaaa
     9421 atccaagaag tcgattacaa acaagttgat ttgctgaaag actttatctc tgaaaacggt
     9481 aaaatcattc ctgcacgcat cacaggaacg aaggcattct accaacgcca attggctgtt
     9541 gccgtaaaac gcgcacgctt cctggctctg ctgccttaca ccgaccaaca caaataattt
     9601 tggagattga atcatgcaaa ttattctgtt agaaaaaatc ggcggtctgg gcaacttggg
     9661 cgacatcgta accgttaaaa acggctacgc ccgcaacttt ctaattcccg caggtaaggc
     9721 aaaacgtgcg accgaagcga atatgaaaga gtttgaagca cgccgtgccg aattggaagc
     9781 caaacaggct gaaattttgg cagatgcccg agtccgtcag gaaaaattgg acggtcaaac
     9841 cgttaccgtt gctcaaaaag ccggtgtgga cggccgcctg ttcggttccg ttaccaatgc
     9901 cgacattgct gctgcaatcg ttgctgccgg catcgaagcc gtgaaagcaa atgtacgtct
     9961 gccgaacggt cctctgaaag ccgttggcga gtacgaagtg gaagtggctt tgcacactga
    10021 tgccgttgct aaaattaccg ttgccgtcgt tgccgcaacc gagtaattga aattatcaga
    10081 tgccgtctga aaccctgccg attgtcggaa tgtggtttca gacggtattt gttttgtttt
    10141 ccgtatattc tgcaaaaatc gggatggttg tttttatagt ggattaaatt taaaccagta
    10201 cagcgttgcc tcgccttgcc ttgccgtgct atctgtattg tctgcggctt cgtcgccttg
    10261 tcctgatttt tgttaatcca ctatatttgc ctgtggtctt tgcaggaata gtttgttaac
    10321 gaaatttgat gcataaaatg cgccaaaaaa tattggctgt gttttaatat agtgttgata
    10381 agcgtacctg gcttcaatat actcaatagg atagatctct tcctaaccga aaaaataatt
    10441 gtcttttccc ttgcaaataa ttgaaatcaa cagattacta aaacacagcc aaaatattca
    10501 attgacaaaa acttttctga ttttgagaaa tctgtgaaaa tcaggggatt atttattttg
    10561 aaaatggtat cgggcataaa atttggctgt gtttcaatat ggtgctgata acatactttg
    10621 tttcaaaatg ttcaatagga taacttcctg attgaccgaa aaataatggc cttttccctt
    10681 gcaagcagtt gaaatcaaca gattgtcaaa atacatccta aaaatttaat aacttatata
    10741 tatgattgca aaggttgcgg cttgtatacc ggtcattatg gcggaaatac cgtttgggag
    10801 gcagccgcat cgcgtggaat cggcacggca ggcgatgcgc cgatgccttc gggcgtgaag
    10861 gcggggcaat cggcagtttg gaaaaaggca atggaatctg tgggcagaag aagttaaggg
    10921 taaaagtcgg gcgcacggtc aggttgtctg tccttttgtc ggaacaggag actgggcagc
    10981 tttgtcctga ttaattgctt gtgcaaacgg taaaaaggtt ttttcgtgtg ttttaaagca
    11041 ggttgtacag catttttccg gcaatcaaaa gcaacataat gccgaaggat tttttgagtt
    11101 tggcagaaga aagtttgtgg gcggttttga caccgagcgg ggcaaaggca atggttgccg
    11161 cgctgaggac ggcgacggcg ggcaggtaaa ggaagcccag tgacccttcg ggcaatcctg
    11221 caatattcag gccgttgagc agatacgata ttgcgccgga gagtgcaatc ggccaggcaa
    11281 ggccggatga tgtgccgatg gctttatggg cggggaagcc gcagtggatt aagaagggga
    11341 cggaaagtga accgccgcct atgccgaccc agctcgacat tgtgccgaac agtgtggaaa
    11401 ccgcagtcag tccgggcagt ccgggcagcg ggcgggatgc cgtctgaggg tcggtatgca
    11461 gtgttttgaa tgcgacggcg gttaaaaaca ggatgaagaa aatttgaagc ccgaacgcgg
    11521 ggatatattt tgcggagagt gcgcccgtga atacgccgaa tatcataccc ggcatcatcg
    11581 caaatacggt tttccagtcg accgcctgtt ttttgtgctg ccccaacata ctggaaaagg
    11641 cggtgaagac catgacggcg aaggatgtgc cgacggcgag gtgttgcgcg taaggatgtt
    11701 gtgccaaacc ctgcaaatca agtacccata aaacgacagg gacaatcagc gtgccgccgc
    11761 ctacgccgaa cagaccggca ataaaacctg ccgcactgcc tacggcaagc aggattaaga
    11821 taatgtccca atgccacatt taatccgcct ttctgtattt tttccagaat cggcgtgccg
    11881 ccataatgta gccggacagg ctgtatccga gaaagaacag gaagaggacg agcgacggtt
    11941 cccaagtgac cagaagcagc aataagacgg caagcagcat tccgacaaag gggacttgtc
    12001 tgcggatgtt gatttcttta aaactccaaa aagggatttg gacaatcatc gacaggccgg
    12061 caaacagtgt gatgcccaat gcccaccagt ggacggcggg gaatttttcg acgctgtggt
    12121 tgacccaaat cagcccgaca atcagcgcgg cggcagtcgg actgggcacg ccgataaacc
    12181 agcgtttgtc caccttgccg atgagtgtgt tgaacagggc gaggcgcagg gcggcgcagg
    12241 cgcagtagat gaaggcgacg gaataaccga ttttgccgaa ctgccaaagc tgccatttgt
    12301 aggcaatcag agcgggagca acgccgaagc tgaccatatc ggcaaggctg tcgagctgct
    12361 ccccgaacgc gctttggctg ttggtcagcc gcgccacgcg cccgtccata ccgtccagca
    12421 acatagagat gaataccgcg atggcggcgg tttcataacg tccgtgcatg gattgggtga
    12481 ttgcgtaaaa tgcggaaaac agcgcggcga tggtaaagga gttgggcagc aggtagatac
    12541 tgttttgacg cagggagcgt tgcggggtgt tggtcggggt ttccatagct gaagtgtctc
    12601 acggacgtgg agaatcgata ggggattata acccaaacca ttatcgaaca atacgccctt
    12661 taggatgaag ccctcggagg cggtttgttt ttctgatggc ggatgctttg cggggcggtt
    12721 tgactctgcc tgccaccggg tgtgttgttt cggatttttg ccggatggat attgttgctg
    12781 ggcatttctg ggcttgccgg aaggtatcgg ggggagggca tcggaatatg cggcgcaccg
    12841 ttcaaaccgg ggcggcgttt ccctattttc agggggttgc aggtttcaac aaattgaaag
    12901 atattttttg catttccgac cgtatgctga ttttgaagcg ggataaatta taggacaagg
    12961 tttttcgccc gccgcaaagg ttggccgcaa tgccgtctga aaacaacggc ttgccgctaa
    13021 ggcggcggta tgcggatttg cgctgccgaa atcggtttgc catacccggc ggtgcaatct
    13081 gcggggaggg ataagccgat aaggcgggac ttatcctgtt ttttgtttat aatgcaacag
    13141 aatttggacg aaccgtcggg aatttatcat gatgaaaaag atactggccg tatcggcact
    13201 atgcctgatg actgcggcgg cacgggctgc cgatacttac ggctatctcg ccgtttggca
    13261 gaatccgcag aatgcggacg atgttttgca ggttaaaact acaaaagaag attcgacgaa
    13321 aagcgaagcg tttgccgagt tggaagcctt ttgcaaaggt caggacacgc ttgcgggcat
    13381 tgccgaagac gagccgaccg gatgccggtc ggtcgtgtcg ctgaacaata cctgtgtcgc
    13441 gctggcatac ccgaaagcct tgggcgtgat gcgcgttgaa aacgccgtcg tgattacttc
    13501 tccgcgtttt acgagcgttc atcaggtcgc actcaaccag tgcatcaaaa aatacggcgc
    13561 acagggacaa tgcggcttgg aaacagtgta ttgcacgtct tcttcttatt acggcggaac
    13621 tgtgcgctct ttgattcaaa atctcaaata aaacggaaaa tgccgtctga aagatgttca
    13681 gacggcattt ctatatcgac ggtcaggatt ctttcggatc gggcagcagg ctgttcaaca
    13741 taatggcaag tacggcgcac aagcccacgc cggcaaagct gaagctgccc aatttgagcg
    13801 tcatgccgcc gatgcccgtg gtcagtaccg agctgacgat gaccaggttt ttcggcagca
    13861 tcaaatcgac tttggcatca atcagcgttt tcacgcccaa agaagcaatc gtgccgaaca
    13921 gcagcagcat aatgccgccc attactggca tcggaatgga agccaaaaac gcattgaatt
    13981 tgccgaaaaa cgccatgcag acggcaaaaa ccgccgccca agtcatgatg acggggttgc
    14041 tgtttttggt aatcattacc gcacccgtta cttcgccgta ggtcgtaacc ggcgggccgc
    14101 cgatcagacc cgcaacgcat acgcccaaac cgtcgcctgc aagggttttg tccaagcccg
    14161 ggtctttcgt atagtctttc cccgtcacat tgccgattgc catgatgccg ccgatgtgtt
    14221 cgatggcggg ggcgacggca acgggcagca taaacagtgc agcctgccag ttgacctgag
    14281 gcgtttcaaa atggggaacg gcgaaccagg gcgcgtgtgc aatgcttgcc gtgtccacca
    14341 gtcccatcag cagtgccaaa acataacccg aagcgacacc gatcaagata ggaatcagct
    14401 tcatcatcct gctgccgaaa accgatacga tggcggtaac ggcaaaggta aagccggaaa
    14461 gaatcagcga atcggtatag tcgatgacct gtttgccgtc cgcctgaccc attgccatgc
    14521 tgcttgccgc cacggcaaca gacagaccga tgaccatgat gacggggccg atgacgaccg
    14581 gaggcagcag tttgtgtacc gctgccagtc cgcgccaacg gatcagcgcg gcaaacacaa
    14641 aatacataaa gccggcggca aacagtccga acatggtgga aggcagcccc cattcgccga
    14701 cggagtagat aatcggtgcg ataaaggcaa acgaagaacc aagaaaaatc ggcactttgc
    14761 gtttggttgt gatttggaac agcagcgttc ccaagcctgc gcccaaaagc gcaagagccg
    14821 gattcagacc ggtcagcagg ggaaccagca ccattgcgcc gaatgccaca aataaaatct
    14881 gtgcaccgga aacggcaagt ttcagttggt tcataccatc ttctttcaat cggttcaaac
    14941 ggcacggatt atactggttt gccgagggtc tgcgtaaaaa tacatatggc gggcaacaga
    15001 tatgccgtct gaaaggcaat gtctgtctgt tgcaggatga aagcaggaga aatggagtat
    15061 aatccgacac tttccatatt caatccgcaa gccgtccggc ttccttattt cagacggcat
    15121 tttccggtaa tacggcatga cagaaaaatc ccataaaaaa acagcaaaag gcagggcggg
    15181 aagcccgtcc ccaacgtccg cacgcaacaa gaaagccgac aacggcgcac ggggcaataa
    15241 agtttccgag cggctcaagg cggtcaaaga gttgcagaaa accgaaacca aaaaggcgcg
    15301 tcccgaacat gtcgtcaacc ttatcggcga cgcactgtgg ctgatgggtt tggcggcaac
    15361 cctgtatttg gcgatttccc tgatcagttt cgatatgggc gatccgtctt ggtcgcacag
    15421 ttcgccggtt gtggaagatg tcgccaattg gggcggactg ttcggcgcgt atgttgccga
    15481 tgtcggctat tatcttttcg gctggtcgtt ctggtggtgg atagcggctg cctgcgtcat
    15541 gctgtataaa aattttcgtc tgcacgcaaa acagacggaa aacgaggcat acaaccacaa
    15601 aatcgctgcc gccgcgctgt ttgtcctgac ggtcttcagc cccgtcttgg agtattttgt
    15661 gctgggcgga aaatatgccg actccctgcc tgtcggagca ggcggtatgg tcggcatacg
    15721 cgtcggcgca gtgtttgcgt ggctgctggg gaaatcgggc agcctgctga ttatcttggt
    15781 tgttctgctg ttgtcgttgt ccctgctggt gcagatttca tggctggaat ttttgaacgg
    15841 tgcgggcagg gcggttcaaa accgcctgag tgccttatcc ggcaaggtca tggctttagg
    15901 aaaacgccgg ccgaatagca aaacagacgg tgtcgatacc caaaatacac ggcgcatggt
    15961 aaaagaagcc aagaatatta cggccaaacc cgttgccttg cccgaaggca gcagcagcaa
    16021 ccgcaaatcc gtcgcggttt ccgtcgcgcc gtcgcccaaa attcaggttt ctctgtttga
    16081 agatgacgaa cctcggcagg cgggcgaata ccacaagcct acattgaacc tattgcggat
    16141 tcctgacagc gaacccgtca gcatcaatcc cgccgaattg gagcgcactg ccgaactgat
    16201 cgaatccaaa ctggcagaat tcggcatcgg cgtacaagtc gtatccgcca catccggtcc
    16261 cgtcatcacg cgctacgaaa tcgaacccgc gcaaggtgtt aaaggcagcc aaattgttgc
    16321 cttgtcgaaa gatttggcac gctctatgtc gctgcaatcc gtgcgtatcg tcgaaaccat
    16381 cgcaggtaaa aacacgatgg gcatcgagtt gcccaacgac aaacgccaag acgtgatgtt
    16441 gagcgaaatc ttgtcctcgc ccgtgtttgc cgaagccaaa tccaagctga ccgtcgcgct
    16501 gggcaaagac attgccggta cccccgttgt cggcgacttg gcgaaaatgc cgcacctttt
    16561 ggtcgccggt atgactggtt cgggcaagtc cgtcggcgtg aacggcatga ttatgtctat
    16621 gcttttcaaa gctacgcccg acgaagtccg ctttatcatg atagacccga aaatgcttga
    16681 gttgagcatt tacgacggca ttccgcacct gctctgtccc gtcgtgaccg atatgcgcga
    16741 agcggggcag gcgttgaact ggtgcgtcgc cgaaatggaa aaacgctacc gcctgctttc
    16801 ccacgccggt gtacgcaatc tggaaggctt caaccaaaaa gtcgaagccg caaaagcggc
    16861 aggcaagccg ctgctcaatc cgttcagtct gaaccccgac agccccgagc ctttggaaaa
    16921 actgccgatg attgtggtcg ttatcgacga acttgccgac ctgatgatga ccgaacgcaa
    16981 agccgtcgag cagcaaatcg cccgtctcgc ccaaaaagcg cgcgccgccg gtatccatat
    17041 gattgtcgcc acccaacgtc ccagtgtcga tgtcgttact ggcctgatta aagccaacat
    17101 cccgacgcgt atggcgttta ccgtgcaaag caaaatcgac agccgtacca tcctcgacca
    17161 aatgggcgcg gacgaactgc tcaaatatgg cgattcgctg ttcctccagc ccggcagtgc
    17221 cgaaccgacc cgcctgcaag gcgcgtttgt ttcagacgac gaagtacatc aagtcgtcaa
    17281 ctacgtcaaa tcgcaagccc cagccgacta tattgaaggt ctgctcagcg gcgaggccgc
    17341 gctggaaact gccaatatcg ttaatccgaa tgcagacagc gacgaattgt tcgatcaggc
    17401 agtcgcctat gttttggaaa gcaaaaaaac ctccatttcg tctttgcagc ggcagttgcg
    17461 tatcggctat aaccgcgcgg caaacctgat ggaggcactg gaaaatgcgg gtgtcgtttc
    17521 tcccaccgac ctcaacggca gccgtaaaat tttggcgcac aaggaccatt tgtagcccgt
    17581 attgcaaaat gccgtctgaa cggcggaatt ggcgtttcag acggcatatt atgtttcagg
    17641 cggaacattg tgatatactt gccagctaaa atctcccctt tgcggcaatg cggttcaaat
    17701 atccgtaccg ttgcgctgtt tgcttcccca tgcagggaag aaggtttacc attttatcaa
    17761 cacaacaaat ttaagggctt atgatgagcg taactgttga aactttagaa aatctggaac
    17821 gcaaagtagt gttgtccctg ccttggtccg aaatcaacgc agaaaccgat aaaaaactga
    17881 aacaaaccca gcgccgtgca aaaatcgacg gtttccgccc gggtaaagca cctttaaaaa
    17941 tgattgccca aatgtacggt gcgagcgcgc aaaacgacgt gatcaacgag ctggtgcaac
    18001 gccgcttcca cgatgttgcc gttgcccaag agttgaaagt ggcaggcttc ccccgttttg
    18061 aaggcgttga agaacaagac gataaagaat ctttcaaagt tgccgccatt tttgaagtgt
    18121 tccccgaagt cgttatcggc gatttgtctg cacaagaagt tgaaaaagta accgcttccg
    18181 tcggtgatgc cgaagtggac caaaccgtag aaatcctgcg caaacaacgc acccgcttca
    18241 accatgtcga acgcgaagcc cgaaacggcg accgcgtcat cattgacttt gaaggcaaaa
    18301 tcgacggcga accttttgca ggcggcgcat ccaaaaacta cgccttcgta ttgggcgcag
    18361 gtcaaatgct gcctgaattt gaagccggcg tagtcggcat gaaggctggc gaaagtaaag
    18421 acgttaccgt caacttccct gaagaatacc acggcaaaga cgttgccggt aaaactgccg
    18481 tgttcaccat tacgctgaac aacgtttccg aagcgactct gcctgaagtc gatgcagatt
    18541 ttgcaaaagc cttgggtatt gcggatggcg acgttgccaa aatgcgcgaa gaagtgcaga
    18601 aaaacgtaag ccgcgaagtg gaacgccgcg taaacgaaca aaccaaagaa tccgtaatga
    18661 acgcgctgct caaagccgta gagctgaaag cacctgttgc tttggtcaat gaagaagccg
    18721 cacgcttggc aaacgaaatg aaacaaaatt ttgttaacca aggtatggct gatgctgcca
    18781 acttggatct gcctttggat atattcaaag aacaagccga acgccgcgtg tctttgggtc
    18841 tgattttggc caaactggtt gatgaaaaca aactggaacc gactgaggag caagtcaaag
    18901 ccgttgtcgc caacttcgca gaaagctacg aagatcctca agaagtaatc gactggtact
    18961 acgcagagcc ttcccgcctg caagccccga cttctttggc gatagaaagc aacgtcgttg
    19021 atttcgtttt gggtaaagcc aaagtaaacg aaaaagcttt gtcttttgac gaagtgatgg
    19081 gtgcgcaagc ctgattatcc tgaaaatgcc gtcttagggc agtttttgaa agcaccgaag
    19141 ccgcttttaa agcccgcttt ggtgctttta catcatgaaa ggagacgaaa tgtcttttga
    19201 taactatctt gtccctaccg ttatcgagca gagcggtcgc ggtgagcgtg cattcgatat
    19261 ctattcccgg cttttgaaag agcgcatcgt attcttggtc ggtccggtaa ccgacgagtc
    19321 cgccaatctg gtggttgccc aactgttgtt tttggaaagt gagaatccgg ataaggatat
    19381 tttcttctat attaactcgc cgggcggttc ggtaacggcc ggtatgtcga tttacgacac
    19441 catgaatttt atcaagcccg atgtatcgac tttgtgcttg gggcaggcag caagtatggg
    19501 cgcgttctta ttgtcggcag gcgaaaaagg caaacgtttc gccctgccca acagccggat
    19561 tatgattcac cagcctttaa tcagcggcgg cttgggcggt caggcatccg atattgaaat
    19621 tcacgcacgc gaactgttga aaatcaaaga aaaactcaac cgcctgatgg cgaaacattg
    19681 cggccgcgat ttggcagatt tggagcgcga caccgaccgt gataatttca tgtccgccga
    19741 agaggctaaa gaatatggtc tgattgatca aatcttggag aaccgtgctt ctttacagtt
    19801 ttaataagaa atctgagaga aacaaatgcc gtttgaaaac agttcaggac ggcatttgtc
    19861 ttatagggac tgtataggga tgattttgtt ctggttgcgt ttggagccgt tttcgccata
    19921 attggtttta acatattcct ctacaatagc taggaattca gttgcaatat tatcaccttt
    19981 cagtgtgact ttgcgctccc cgtccacata aaccggcgcg acgggtgttt ctcccgtacc
    20041 gggcaggctg atgccgatgt cggccaattt gctttctccg ggcccattga caacgcagcc
    20101 catcacggca acattcaggg attccacgcc tggataagca gtacgccata cgctcatttt
    20161 ttgtcgcagg taattttgaa catcttgtgc cagctcttga aatacggtac tggtggtacg
    20221 cccgcagccg gggcaggcgg taaccatcgg cgtaaacgaa cgcaatccca tagtctgtaa
    20281 aatctcttgc ccgacgacga cctcctgagt acgcgggctg ccaggttccg gagtcagtga
    20341 aatgcggatg gtgtcgccga ttccttcttg aagcaagacg gataatgccg ccgttgatgc
    20401 gacaatgcct ttgctgccca taccggcttc ggtcaaaccc aaatgcagcg gataggcgca
    20461 acggctgccc agttcgcggt aaacctgaat caaatcctga accgcgctga ctttgcacga
    20521 caggatgatt ttgtcttcgg gcagtcccaa tagaacggct ttttcggcag attccaaagc
    20581 ggagacaatc agtgcttcct tcgtcacttc ttcgggcggt ttcggcgcgg aagaagcgag
    20641 gttggcatcc atcatacgtt tggcgaggct ttgatccaaa gaaccccagt ttacgccgat
    20701 gcggacggct ttatcgtttt cagcagcagt ccgaatcata aaggcaaatt tttcatcgcc
    20761 ttttacgcct ttgccgacat tgccgggatt gatgcggtat ttggacaatg ctttgccgca
    20821 ttctggaaat tccgccaaca ggcgttcgcc gttgaagtgg aaatcgccga taagcggtgt
    20881 ggcatagccc atatcgtcca agcggcggcg gatttcggca actttggacg cggcttcggg
    20941 gctgttgacg gtaatacgca ccatttcgga tccggcatcg ctcaattcct taatctgcaa
    21001 tgcggtggct tttgcatcgg cagtgtcggt gttggtcata gattggataa cgacgggtgc
    21061 ttctgaaccg acggtaatat gatcgatgcg gacttgatgc gtcttgcggc gttggagtgt
    21121 gttcatatgg tttgaatctg tttattgacc ggtgaggacg gtttgcaatt cttccgagta
    21181 ggggaaattc gcctgcaatt gtgcttcata ttcgtatgcc gcctgtgcgt tgccgagggc
    21241 tttggcaatt ttccagccta gcagcaaatc atcggcctga aggacttcta ccctgctttg
    21301 gtatttttta aagtagtaat cggcatcgcc caactgcccg gccagcattt tggtgcgcgc
    21361 cagttcttta aatgcgggtg ggaactgcgg ctgggcggcg agggaacgtt tcaaataggc
    21421 ttccgccaat ccgaattgcc cctgttttgc gctgcatatg cctttattca ggttggcaat
    21481 ataaggggtc gggtaggtgg ggtcggccag ggctttgtcg aaatatgcca tagattcggc
    21541 agggcggttg agcctgccgc acaggaacca accgtagttg ttgttgattt cggcactgtc
    21601 gggtttgatg gagagggctt gccggaaact tttctgcgcc ttgtcgttaa ctttcaggta
    21661 ttggtagatt tcggcacgga ccagccaggc aagctcgttt ttagggtctg atttcagggc
    21721 gtcttcaata cttgccgtcg cctgacggta gtcctgaccg cgcatatatt ccattgccaa
    21781 ctgggttttg atattggaaa cctgattggc tttttctgcc cgcgaggggc ggtaggaagt
    21841 gctgcacgcg cccaaggcaa ggacaagtaa taaagagatt cgtttggatg gcttaaaagg
    21901 cataattacc cctgttgtcc gattaaaatc tgctgccatt tttgttggcg gcgcgtttta
    21961 tcctgaacct gccccgccaa ctgtccgcag gcggcatcga tgtcgtcgcc gcgcgttttt
    22021 cgtacggtaa cgacaaatcc tgcctgctgc aaaatatcgc ggaacacacg gatgttctca
    22081 ttgctggagc gttcgtatcc ggagtttggg aagggattga acggaatcag attgaacttg
    22141 cagggaacat ctgtgaccag ttcgatcagt tcgcgcgcat gttgcgcctt atcgtttatt
    22201 ccgtccaaca tgacgtattc gaaagtgatg aaatccctgg gtgctttgac cagatagcgt
    22261 tggcatgcgg ccatcaattc tttcaaggga tattttttgt tcaacggtac gatttggttg
    22321 cggacttcgt cattggaagc gtggagggaa accgccaaag ccaccggcat gacatcgcgc
    22381 aacctgtcca tttggggaac catacccgaa gtggaaacgg ttacgcggcg gcggctcaaa
    22441 ccgtagccgt ggtcgtccag catgatgctt aaggcggtaa cgacattgtc gaagttcgcc
    22501 atcggctcgc ccatgcccat catgacgacg ttggaaatca cgcgctcgtt tttcggtgta
    22561 acgcccatcg ctttgtttgc ccaccacaat tgcccgatga tttcggcagc agtcaaattg
    22621 cggttgaagc cctgccggcc ggtcgaacaa aatgtacatt ccaaagcgca gccgacttgt
    22681 gaggaaatgc agagcgtgcc gcgatccgat tcggggatga agacggtttc caccccgttg
    22741 cccgtaccga catccaaaag ccattttcga gtgccgtctg aagatttttg agacatcatc
    22801 agcttgggaa tttcgatgcc tgcctgttcg ttcagtttat ggcgcaacga ttttgccaaa
    22861 tcggtcattt cgtcaaaatt ttgcgcgccg gattggtgca tccaacgcat aacctgtttg
    22921 gcacggaaag gtttttcgcc catatcggca aaatgtcggg tcagcccttg gaggtcgtag
    22981 ttgagcagat tggttttcat gtatttgtgt ttttcttaaa ttaaggcttc aaataaaaaa
    23041 ttgcagggca ggcaagtttg atttgaagcc ttttgcaaca gagttttcag acggcatgga
    23101 tatattttat gccgtctgaa gaggagttgt gcggtgtatt gtatcaacgg gggcagattt
    23161 cggtttggct gaaaaagtag gcaatttcca aagcggcatt ttccggagtg tccgagccgt
    23221 gtacggcatt gatgctgacc gaagtggcaa agtccgcgcg tatcgtgcct tcggcggctt
    23281 cggcgggatt ggtcgccccc atcagttcgc ggtttttcag aacggcgttt tcgccttcca
    23341 gtacttgaat cataaccgga ccgccggtca taaattcaac caatccggcg tagaaggggc
    23401 ggtctttatg aaccgcataa aattcttgcg cctctttgag ggtaagctgc ttcattttgg
    23461 cggcaacgat tttcagaccg ttctcctcaa agcggctgta tattttgccg atgacgtttt
    23521 tgccgacggc atcgggtttg atgatggaga tggtacgttc aatcgccatg ctatatcctt
    23581 atttttactt aagaagaatc aaatcggtat tctatcaaaa aaataactta tccgcctgaa
    23641 tccttgggag atagggtcag gtgcggctgt tccaaataaa cttcggactg catttcgacc
    23701 atccggctgg cggtgcgggt aaattcttcg gcaaaatcgc cttccgtata gatatggttg
    23761 acatctaccg cgccggtggc acacagtttg acccggaaat cgtagagtac gtcaatcagc
    23821 caagtcagcc gccgcgcctc cgccttttct tgcggtgaga gttgttccaa acctgaaata
    23881 aaaaccattt cataatgttc ggccaaatac agatagtcgg actgtgagcg ggggccgaag
    23941 cacagtgcgc ggaaatcaaa ccatatggca cggccggact cggctttgtg gggaatctcc
    24001 cgaccgtgga tggtgctgat gccggggttc aaatcggtaa tgcctgtcat ttctttgaac
    24061 agttttgcca gttttgcctc attttcttca ttggcaggcg taaagaaaat ctcggcgggg
    24121 cggagggtac gcagtcggta gtcttcaccg ccgtcaacgt ttaagacggt caggctggac
    24181 tcgatgagcg cgattgtggg aagaaaactg ctccggtttt gaccttgcgg gtagagttcg
    24241 gaaggcgcgt agtttgaagt cgccaccaaa acaacgccct cgttaagcag gttttccagc
    24301 agacggccta aaatcattgc atccgcaata tcgctgacat gaaattcgtc aaaacataat
    24361 acgcgggttt ctttggcaat ctcggcggca acggatttca acgggttgct ttcgcttttc
    24421 agggttttca gcctctggtg gatttctgcc ataaaggcat gaaagtggac gcggcgtttg
    24481 cggcggtaag ggaggcagcc gaaaaaagcg tccatcagaa agcttttgcc gcgtccgacc
    24541 ccgccataga aataaagccc tttggggact tgcggggaac gcaaactcct gcctaaaaaa
    24601 cggtttcttt tgcgtttgaa catcatcaat tcggtccaaa gccgatcgag gtgttcgatg
    24661 gcggctgcct gcgcgtcgtc gcggatgaag ttgggcagtt gtgaggcagc ctgataccag
    24721 gtcagcgggc tgtggttttc aaacggcggg gctttaaaaa gttggtctct attactcata
    24781 tttaaccttg ttatacttct tgcggcggag aaagaaaatg cttaccgtcg gtattataaa
    24841 tgatttcctt caaacttggc attttccgtg gaaaagaata atgacgcgca caagggggta
    24901 taagcaaagg cggcgtttca atatggtgca gatatgagct caaatgcctt ttatagtaga
    24961 ttaaatttaa accagtacag cgttgcctcg ccttgccgta ctatctgtac tgtctgcggc
    25021 ttcgtcgcct tgtcctgatt tttgttaatc cactataatc cgtagagtcg gctccttccg
    25081 gctcagacgg catacgttta tgccggcact tcttaccgcc ctcgtgccgg ctatcccaaa
    25141 actgctttga acactgaacg ctctttggtt tacttcaaaa acgcggcgtg ataagcaata
    25201 tgttcgccaa taaaactggc gatgaagtaa taactatggt cgtagccttt gtggaagcgc
    25261 acatcgaccg gctggtttgc cgcacgacag gtttcgataa aatcttcggt acgcaattgt
    25321 gtcggcaaaa actcatcttc caagccctga tcaatgcgta taccttgcac tttatagcct
    25381 tgttgaatga gcgagctggc atcatattgc tgccattttt cacgatcttc ccctaaataa
    25441 gcagtaaagg ccttttctcc ccaaggcacg aggcttggcg ataaaatagg cgaaaaagca
    25501 gaaacgcttt gataacgttt ccgatttcgc agtgccaata ccaatgcacc gtgtccgccc
    25561 attgaatgcc ccataatgga acgtttgccg ttagtagaga aatttgcttc aaccaaatca
    25621 ggcaattcgt tcaagatata atcatacatt tgataattcg tcgcccaagg ctgctcagtt
    25681 gcattaagat aaaagcccgc accttgtcct aaatcgtaag ccgcatcgtt cggcacttgc
    25741 tctccgcgag ggctggtatc aggggcaact acaatcactt gatgttctgc tgcataaagc
    25801 tgaaagcctg atttagtaat gaaattttgt tccgtacacg tcaagccgga aagccaataa
    25861 atcacaccaa gcggttgatt ttccgaatta tttggcaaat agacggcaaa tttcatctcg
    25921 cattgaagcg tttgggcatc atgagcccaa acttgctgca aaccgccaaa aatttgatgt
    25981 tgttcaatca gtttcatcgc ataccttagt agtgaataac ggcgcggatc gatttacctt
    26041 cgtgcattaa gtcaaaggct ttattgattt gatcgagtgt cattgtgtgg gttacaaacg
    26101 gttctaactc aatgtcgcct ttcattgaat cttccaccat tttcggcagt tcagagcgac
    26161 ctttcacacc gccaaatgct gaacctttcc acacacggcc tgttaccaac tggaacggac
    26221 gcgttgaaat ttcttgtcct gcacctgcta cgccgatgat aatggattga ccccaaccac
    26281 gatgtgcact ttctaatgct tgacgcatta cgtttacatt gccgatacat tcaaaggtat
    26341 ggtcaatgcc ccatttgttg atgtctaaca acacatcttt aattggttta tcgtaatcgt
    26401 tcgggttcaa acaatccgtt gcaccgaact gttttgccaa ctcaaatttt gatggattgg
    26461 tatcaatggc gataatgcgg ccggctttgg cttgacgcgc accttgcacc accgccaaac
    26521 caatcgcgcc taagccaaac acggcaacag agtcgccttc ttgcactttt gccgtattat
    26581 gtaccgcacc aatacctgtg gtaacgccgc agccgagcaa acatacttgt tcatggttgg
    26641 cttcagggtt gattttcgcc agtgaaactt cggcaacaac ggagtattca ctgaaagtcg
    26701 aacagcccat atagtgataa atcggctgac cttgataaga aaaacgcgtc gtgccgtccg
    26761 gcattaagcc tttaccttgt gtatcacgca ctgagacgca caagttggtt ttacctgaac
    26821 aacaaaactc acattcgcca cattcggcgg tgtaaagcgg aatcacgtga tcacccggtt
    26881 ttacgcttga cacaccttcg cccacagcaa cgaccacacc cgcaccttcg tgtccaagca
    26941 ccacagggaa tacgccttca ggatcgcttc ctgataatgt aaacgcatca gtatggcaca
    27001 cgccagtgtg ggtattgcgg attaacacct cgcctttacg cggcatttct acgtcgattt
    27061 ccacaatttg taagggttgg ttaggggcga atgccaccgc cgcacgagat ttgatgcttg
    27121 aatcggtttg tttcatttcc attgtctttc ctcttaattc attgacaaag ttgccttacc
    27181 tgagcaacat agcaacagtt tacactttag agttaactct aaggcaagtg ttttttactg
//
LOCUS       DAWHXS010000013        26865 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWHXS010000013 DAWHXS010000000
VERSION     DAWHXS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.013"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            108..323
                     /gene="rpmE"
                     /locus_tag="WIE22_000442"
     CDS             108..323
                     /gene="rpmE"
                     /locus_tag="WIE22_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ4126584.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            639..1268
                     /locus_tag="WIE22_000443"
     CDS             639..1268
                     /locus_tag="WIE22_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ4126585.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            1414..3075
                     /locus_tag="WIE22_000444"
     CDS             1414..3075
                     /locus_tag="WIE22_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ4126586.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
     gene            3192..3797
                     /locus_tag="WIE22_000445"
     CDS             3192..3797
                     /locus_tag="WIE22_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ4126587.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3869..4261
                     /locus_tag="WIE22_000446"
     CDS             3869..4261
                     /locus_tag="WIE22_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ4126588.1"
                     /translation="MPTSTKPYVLRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(4290..4439)
                     /locus_tag="WIE22_000447"
     CDS             complement(4290..4439)
                     /locus_tag="WIE22_000447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126589.1"
                     /translation="MPSEARIAGSDSIFNVPISNRQGQHGFNTPKQVLPINGFRLRPI
                     GQNAV"
     gene            complement(4460..4906)
                     /locus_tag="WIE22_000448"
     CDS             complement(4460..4906)
                     /locus_tag="WIE22_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ4126590.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSVKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(5038..5250)
                     /locus_tag="WIE22_000449"
     CDS             complement(5038..5250)
                     /locus_tag="WIE22_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ4126591.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5539..7386)
                     /locus_tag="WIE22_000450"
     CDS             complement(5539..7386)
                     /locus_tag="WIE22_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ4126592.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAVATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSHVGAQGLMQVMPATAREIA
                     GKIGMDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            7917..8654
                     /locus_tag="WIE22_000451"
     CDS             7917..8654
                     /locus_tag="WIE22_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ4126593.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKTRVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            8656..9342
                     /locus_tag="WIE22_000452"
     CDS             8656..9342
                     /locus_tag="WIE22_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ4126594.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKAVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIIRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            9501..10364
                     /locus_tag="WIE22_000453"
     CDS             9501..10364
                     /locus_tag="WIE22_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126595.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(10427..10996)
                     /locus_tag="WIE22_000454"
     CDS             complement(10427..10996)
                     /locus_tag="WIE22_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ4126596.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSTEWQGMAD"
     gene            11154..12014
                     /locus_tag="WIE22_000455"
     CDS             11154..12014
                     /locus_tag="WIE22_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ4126597.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            12150..12488
                     /locus_tag="WIE22_000456"
     CDS             12150..12488
                     /locus_tag="WIE22_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126598.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            12856..13209
                     /locus_tag="WIE22_000457"
     CDS             12856..13209
                     /locus_tag="WIE22_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ4126599.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            13199..14065
                     /gene="atpB"
                     /locus_tag="WIE22_000458"
     CDS             13199..14065
                     /gene="atpB"
                     /locus_tag="WIE22_000458"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ4126600.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            14122..14358
                     /gene="atpE"
                     /locus_tag="WIE22_000459"
     CDS             14122..14358
                     /gene="atpE"
                     /locus_tag="WIE22_000459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ4126601.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            14429..14899
                     /locus_tag="WIE22_000460"
     CDS             14429..14899
                     /locus_tag="WIE22_000460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ4126602.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            14904..15437
                     /locus_tag="WIE22_000461"
     CDS             14904..15437
                     /locus_tag="WIE22_000461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ4126603.1"
                     /translation="MAEFSTIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAIIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            15448..16995
                     /locus_tag="WIE22_000462"
     CDS             15448..16995
                     /locus_tag="WIE22_000462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ4126604.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            17020..17895
                     /gene="atpG"
                     /locus_tag="WIE22_000463"
     CDS             17020..17895
                     /gene="atpG"
                     /locus_tag="WIE22_000463"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ4126605.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            17933..19330
                     /gene="atpD"
                     /locus_tag="WIE22_000464"
     CDS             17933..19330
                     /gene="atpD"
                     /locus_tag="WIE22_000464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ4126606.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            19341..19763
                     /locus_tag="WIE22_000465"
     CDS             19341..19763
                     /locus_tag="WIE22_000465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ4126607.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            20168..21073
                     /gene="glyQ"
                     /locus_tag="WIE22_000466"
     CDS             20168..21073
                     /gene="glyQ"
                     /locus_tag="WIE22_000466"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ4126608.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            21150..21560
                     /locus_tag="WIE22_000467"
     CDS             21150..21560
                     /locus_tag="WIE22_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ4126609.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLEVNDFGMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLHTTPLVDGFYGDDGWENYSETYKQAD"
     gene            21581..23644
                     /locus_tag="WIE22_000468"
     CDS             21581..23644
                     /locus_tag="WIE22_000468"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ4126610.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            23661..24707
                     /locus_tag="WIE22_000469"
     CDS             23661..24707
                     /locus_tag="WIE22_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ4126611.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            24749..25576
                     /locus_tag="WIE22_000470"
     CDS             24749..25576
                     /locus_tag="WIE22_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ4126612.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQV
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISK
                     KAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            25588..25683
                     /locus_tag="WIE22_000471"
     CDS             25588..25683
                     /locus_tag="WIE22_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ4126613.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            25725..26567
                     /locus_tag="WIE22_000472"
     CDS             25725..26567
                     /locus_tag="WIE22_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ4126614.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         6400 a         7013 c         7020 g         6432 t
ORIGIN      
        1 attttatcga atatcctctt gacgaaacaa cagaaaatag cgagaatgcc gagcttgtct
       61 gaagcatagg tacatcccct atgcccgctt ttttgaaagg aaatcaaatg aaacaaggta
      121 ttcacccgaa ctaccacgaa gtcaacgtta cctgctcttg cggcaacaaa ttcgcaacca
      181 aatccgcgat ggaaaaagaa aacttcaata tcgaggtttg ctccctgtgc cacccgttct
      241 ataccggcac ccaaaaaatc gtcgatacca ccggccgcgt ggacaaattc aacaacaaat
      301 tcggcaacct gttcaaacgc tgattgccgc tttgcaagaa ttaagccctg ccatcatttc
      361 gatggcaggg ctttttaata tttggcacac tgacttctta tctatatagt ggattaacaa
      421 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggaatc gattcacttg
      481 gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg
      541 tttttgttaa tccactatat tttgatgggg ttgaagttga catctacaaa gtttaatgtt
      601 tgaatacatt aaaaaaatat atttgaatga gatgttttat gattcaaaat gttgttactt
      661 caataatcct gtattctggg acagccgtag acttacttat tatcctaatg ttattttttg
      721 ccaaaagaaa aagcagaaaa gacatcatta acatctattt aggacaattt ctaggctctg
      781 ttagcctgat attgctaagt ttgctttttg catttgtctt agattatatt cctagtaaag
      841 agattttagg tttgctcggc ttgattccaa ttttcctagg catcaaagtt ttgctgttag
      901 gagattctga tggagaggct attgccaaag agggtttgcg caaagataat aaaaacctga
      961 tttttctagt cgctatgatt acttttgcaa gttgtggtgc tgacaatatt ggtgtctttg
     1021 ttccatattt tactacctta aatttagcga atttgatagt ggctttactt acctttctag
     1081 tcatgattta tctcttggtt ttttctgccc aaaaattagc acaagtccct tctgttggag
     1141 aaactttgga aaaatatagc agatggtttg ttgctgttgt ttatttggga ttgggggtat
     1201 atatcctgat tgaaaacaac agttttgata tgctatggac tgtgtcgggc caggaaaaaa
     1261 tattatgaaa aagatagtat ctgcaaagac tgccatgtgc agtctttttt gttgccggtc
     1321 tttttgtgtc tgatgccgtc tgaagcagtc tctgcacgac ctttgtgcga atatttgcta
     1381 cacttggcaa tccgtccatt tcttctcacg cttatgctga cctacactcc gcccgatgcc
     1441 cgcccgcccg ccaaaaccca cgaaaagccg tggctgctgc tgttgatggc gtttgcctgg
     1501 ttgtggcccg gcgtgttttc ccacgatttg tggaatcctg ccgaacctgc cgtctatacc
     1561 gccgtcgaag cactggcagg cagccccacc cctttggttg cccatctgtt cggtcaaacc
     1621 gatttcggca taccgcccgt gtatctttgg gttgccgccg cgttcaaaca tttgctgtcg
     1681 ccgtgggcag ccgacccgta tgatgccgca cgctttgccg gcgtgttttt cgccgttgtc
     1741 ggactgactt cctgcggctt tgccggtttc aactttttgg gcagacacca cgggcgcagc
     1801 gtcgtcctga ttctcatcgg ctgtatcggg ctgattccag ttgcccattt cctcaacccc
     1861 actgccgccg cctttgccgc cgccggactg gtgctgcacg gttattcttt ggctcgccgg
     1921 cgcgtgattg ccgcctcttt tctgctcggt acgggctgga cgctgatgtc gttggcagca
     1981 gcttatccgg cagcatttgc cctgatgctg cccttgcccg tactgatgtt tttccgtccg
     2041 tggcaaagca ggcgtttgat gttgacggca gtcgcctcac ttgcctttgc cctgccgctt
     2101 atgaccgttt acccgctgct cttggcaaaa acgcagcccg cgctgttcgc gcaatggctc
     2161 gacgatcacg ttttcggtac gttcggcggc gtgcggcacg ttcagacggc attcagtttg
     2221 ttttactatc tgaaaaacct gctttggttt gcattgcctg cgctgccgct ggcggtttgg
     2281 acggtttgcc gcacgcgcct gttttcgacc gactggggga ttttgggcat tgtgtggatg
     2341 cttgccgttt tggtgctgct tgccgtcaat ccgcagcgtt ttcaggataa cctcgtctgg
     2401 ctgcttccgc cgcttgccct gttcggcgcg gcgcaactgg acagcctgag gcgcggcgcg
     2461 gcggcgtttg tcaactggtt cggcattatg gcgttcggac tgtttgccgt gttcctgtgg
     2521 acgggctttt tcgccatgaa ttacggctgg cccgccaagc ttgccgaacg cgccgcctac
     2581 ttcagcccgt attacgttcc cgacatcgat cccattccga tggcggttgc cgtactgttc
     2641 acacccttgt ggctgtgggc gattacccgc aaaaacatac gcggcaggca ggcggttacc
     2701 aactgggcgg caggcgttac cctgacctgg gctttgctga tgacgctgtt cctgccgtgg
     2761 ctggacgcgg cgaaaagcca cgcgccggtc gtccggagta tggaggcatc gctttccccg
     2821 gaattaaaac gggagctttc agacggcatc gagtgtatcg gcataggcgg cggcgacctg
     2881 cacacgcgga ttgtttggac gcagtacggc acattgccgc accgcgtcgg cgatgtacaa
     2941 tgccgctacc gcatcgtcct cctgccccaa aatgcggatg cgccgcaagg ctggcagacg
     3001 gtttggcagg gtgcgcgtcc gcgcaacaaa gacagtaagt tcgcactgat acggaaaatc
     3061 ggggaaaata tataaaaaac aacagattga gccgaatttc tggattaagt gccggaaatc
     3121 gcgtataatt gcgcgattaa acctttataa atcagccgtt ttacaggcat cacacaggag
     3181 cgacaattat tatgatgacc ctctattccg gcattacctg ccccttcagc caccgctgcc
     3241 gcttcgtttt gtacgaaaaa ggcatggatt ttgaaatcaa agacgtcgat atttacaaca
     3301 agcccgaaga cctcgccgtc atgaacccgt ataaccaagt tcccgtgctg gtcgagcgcg
     3361 atttggtgct gcacgagtcc aacatcatca acgaatacat cgacgaacgc ttcccccatc
     3421 cgcagcttat gcccggtgat cccgttatgc gcggcagggg ccggctggtg ctgtaccgta
     3481 tggaaaaaga attgttcaac cacgtccaag ttttggaaaa ccccgccgcc accaacaagg
     3541 aacaggcaaa agcgcgcgaa gccatcggca acggtctgac catgcttgcc ccttcgttca
     3601 gcaaaagcaa atacatcctc ggcgaagatt tttctatgat tgatgtcgcc cttgctccgc
     3661 tgctgtggcg gctcgaccac tacgatgtca aactgggcaa aagtgccgcg ccgctgctca
     3721 aatacgccga gcgcatcttc cagcgcgaag cctttatcga agcactgaca cccgccgaaa
     3781 aagccatgcg caaataagtc cgaaatgctt gcaaaaccca ccgttttgca ggcattttcc
     3841 tattttggcg tacaacacgg aacccatcat gcccacctca accaaaccct acgtcctccg
     3901 cgctctctgc gaatggtgca gcgacaacag cctcacaccg cacatccttg tttgggtcaa
     3961 cgaacacacg cgcgtcccca tgcagtatgt ccgcgacaat gaaattatgc tcaacatcgg
     4021 tgcgaccgcc acgcaaaacc ttcaaatcga caacgattgg atcagctttt ccgcccgttt
     4081 cggcggacag gcgcacgaca tatggatacc tgtcggacac gtcctcagcc ttttcgcacg
     4141 ggagaccggc gaaggtatgg ggtttgaatt ggaggagtac cgccccgaca cgccgcctga
     4201 aaacacctct gccgaaaccg cgccccgacc cgccaaaaaa ggcttgaaat tggtcaaata
     4261 aatctatgcc gtctgaacgg aatcgtgttt cagacggcat tttgtccgat ggggcgcaaa
     4321 cggaatccgt ttatcggcaa aacctgtttc ggcgtattga aaccgtgttg cccctgccga
     4381 ttcgatattg ggacattgaa aatgctgtct gaacctgcga tacgggcttc agacggcatt
     4441 ttgtccgata ttcgggcaat caggcggtca gcacggcttt caggattttg ttgacttcgc
     4501 ccatatcggc tttgccggca agtcgggttt tcaatacgcc catcactttg cccatatccg
     4561 ccatacctgc cgcgccggtt tcggcaacgg cggcatcgac cgcagtacgg atttcgccgg
     4621 cggagagcat ttgcggcagg tagcggtgca gtacctcgat ttcggcgttt tctttgtctg
     4681 ccaaatcctg acggccggct tcagtgtaga ttttcacgct gtctttgcgc tgtttgacca
     4741 ttttggtcag gatggcggtg attttggcat cgtcggcttc ggtgcgttcg tccacttcaa
     4801 actgtttgac ggcggcgttg atgaggcgga tggtgccgag ggaaacttgg tctttggcgc
     4861 gcatcgcggt tttcatgtct tcggtaaggc ggattttcag gctcataatg ttctcgctgg
     4921 gatgtcggat ggaaacggcg ggtttcgatg ccgtctgaaa agcaaaacac accgcagagc
     4981 aggtcttgcg gtgtgtcctc atatcacagg gctgacctgt aatctgtact tgaacgttta
     5041 gtacattttg ggcggcagtt gttggctgcg caggcgtttt tgcaggcgtt ttacggctgc
     5101 cgcttttttg cgtttgcgtt cggtagtcgg tttttcgtag gcttcgcggg cgcgcagctc
     5161 ggtcagcagg ccggtttttt ctacggcgcg tttgaaacgg cgcatagcga cttcaaaggg
     5221 ttcgttttct tttacgcgga ttgcaggcat tttatttcct ttaataaatt ctgttgttgc
     5281 atctgcccat catatcggtg gaaagggtag ggcagacggt gttgaaggtt taccgtatcg
     5341 ctgtgccttg cggcggcggg atacgcgccg tgacggaaac atcacctcct aacgggtcgg
     5401 cttgtgccgt gtcaagctgc ttgaaaaagc aggaaaaaca attttcggat tgtcttatat
     5461 ttatggttcg ccgtcaatgc cgttcgggat aaaaatgccg tctgaaagac cgggcgggtt
     5521 tcagacggca tcggtacgtc agcgtgcagg aacaatgccc atacgctgtt tgagcgggat
     5581 gtgcggcgcg ccgaagaggg cggcgtagta ggcggcattg gtcatcactt ttttgacata
     5641 gtcgcgcgtt tcggaaaacg ggatggtttc ggcatatacc gcgccttcga ggggcgtgtc
     5701 cgcctgccat cggcgcgccc tgccgggacc ggcgttatag cctgcggtgg cgaggacttc
     5761 gttgttttgc aggcggcgtt tggtgtccgc catataccac gtccccatac ggatattgcc
     5821 gtcggcggtg taaaattgtg cggcatccat accgattttg ccggcgattt cgcgcgcggt
     5881 ggcaggcata acctgcatca gcccctgcgc gcctacgtgg gattgcgcgc ctataacgaa
     5941 gcggctttcc tgacgaatca gaccgtaaac ccaagccgga tcgacattaa cattttgcgc
     6001 gtggcggatt accgtgtctt taaacggcga aatatagcgc aaggtgtagt tgagtttgcg
     6061 gtcggtgcgt tccgcgctgt tgaccgccat atcgtaaaaa ccgtggtcga acgcggtttg
     6121 cgcggcggtc agcagcttgt cttcgtcaaa gccgcgtgtg gcaaaacgcc attccgcctg
     6181 agcctgacgg cgcattttcg catcgccggc ggttcggctg ttttggaaca gtaccagcgc
     6241 gcgcttaatc gcgccgtctt ccgccatacg gaggacgctg tttttgccgg catcgggaac
     6301 attgttgcgc gtatcgattt tgcgccccaa ctcttcccct gccagtacgg cataaaaatt
     6361 cctgcccgtc gctaccgcct gtttgtagag tttttccgcc tcttgcgtgt tgcccgttgc
     6421 ggcgcggctg cgtgccagcc agtagagcca ggtcgggctt ttttgcagtt tttcgggcat
     6481 atgcgagatt acggaagcca gctcgtccca acgtcgggcg cgcaaggcgg cgcgggcgta
     6541 ccactcgatt tggtcgtcgg tcagttggcg gcggtcggca accttgccgt aatagtccaa
     6601 ggcggcaggc acattgaggt tttgcgactg ataatgcccc aatacgcccc acgcgaaact
     6661 gcgttgttcg aggcttaaac cgctttccat ttcggacagc agggcggcgg cattcggcga
     6721 tttgcgtgct tctttgccga tgacgttcaa cagggcatat tcgcgcgaac cttgtgtacc
     6781 gccgtcaaac gggctgccca atgcggcggc aaggttgcgt gcgtctgtgg tttggcggcc
     6841 ggccagcagt ccgcgcacgc gcctccaggc gtcgttgccg tccaacaagc cggatgcggc
     6901 tgcctgttcc aacagtttgg tgcagcccga aggcagtttg cccgtatttt tgaccagttc
     6961 ggcggcacgc gtatagtcgt tgcggctcga atcggcgtag cattcgactt cttgggcgcg
     7021 cccttcaggt ttgagtttgg catattcctg tgcaaacagc gtccactgtc tgcgtgcgcc
     7081 caaagacttc agccactcgt tgcggacatt ttccgccatc gcgctgtcgc cggcgttttc
     7141 caaataggcg gcgacggcgg catcgttttt ctgtttcact gcatccagtg cggaagggta
     7201 gctgctgtaa tctgccagcg tttttccttc gggttcggca gggcgggtgg gaacgcttgc
     7261 cgaaagaccg gcagtttcta tattgtctgc cagggtcttg ctgctgggcg cgtgtgtcga
     7321 agaacacgcg gcaagcacca gggccgccag cagcggcagg gaatgcttca tagagggtag
     7381 gtacatcgga tttccttaag aatcggaacc ctgaacggtc agggttggaa aagacaaaat
     7441 gccgtctgaa caggcgtttg cccgaattat atgccgaaac tgcaccgcct ttggaatgtt
     7501 tccgacataa tttatagtgg attaaattta aaccagtacg gcgttgcctc gccttagctc
     7561 aaagagaacg attctctaag gtgctgaagc accaagtgga tcggttccgt actatttgta
     7621 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatatg tttttcaatt
     7681 atttgccgtt ttggtgcgaa ccgctgcctt tgcccgtttc agacggcatt gtccgaaatg
     7741 gttgcccgct tcctgcttta ttgacaaaaa aatgctttcc cgataatatc ctacgaaaat
     7801 taacctgccg atttgacaca gcttgcgggc ataacagcta aagcgttccg acaatttcag
     7861 ctttatcttc cgcgcccgtt gtgtccgaca tcgggctttg ttgtatggaa aagacaatga
     7921 ttattttgga caaggtttcc aagcattacc aaacgcgcga caagacccgt tttgccgccg
     7981 tcgagccgac cagcctcgaa atccgcgacg gcgaaatctt cgggctgatg ggttattcgg
     8041 gtgcaggcaa atccaccctg ttgcgcctga ttaacctgtt ggaacgcccc gacagcggca
     8101 aggtcaacgt ctgcggacaa gagctgaccg cgcttgatgc cgccgcattg cgtcaggcgc
     8161 ggcagaatat cggcatggta tttcaacagt tcaacctgtt gaacaaccgc accgtcgccg
     8221 gcaacgtcgc cttcccattg gaaatcgccg gatggccgtc tgaaaaaatc aaaacgcgcg
     8281 ttgccgaatg cctcgaaatc gtcggactga ccgaacgcgc cggccactat cccgcccagc
     8341 tttccggcgg gcagaaacag cgtgtcggca tcgcccgcgc actcgcgccc aaaccccaag
     8401 tcatcctcgc cgacgaaccc acttccgccc tcgaccccgc caccacgcgc agcgtcttgg
     8461 aatgtttgga agacatcaac aaacgattca acgtaaccat cgtcatcgta acccacgaaa
     8521 tgagcgtcat cagacgactg tgcgaccgcg ccgccctgtt ggacaaaggc aaagtcgtcg
     8581 aaatcgtcga agtacgcggc aaccaaatcc acgcccgatc cgacatcggg cgcgaactga
     8641 ttcgggagga ctgatatggc agacttaaca ttccaacaag ccgtttccaa catcatcggt
     8701 atgaaaggcg aaatcgtccg cgcattgggc gaaaccttcg tgatggtcgg cttgtccacc
     8761 acattcgccg tcgttttcgg cacgttgctg ggcgtgttgc tcttcgtaac ttccagccgc
     8821 caactgcatt acaacaaggc ggtgaacttc ctgctcgaca acctcgtcaa cctcatgcgc
     8881 gccttcccct tcgtcatttt gatgattgcg atgatacccg ccacacgcgc catcgtcggc
     8941 agcaccatcg gtccggttgc cgcctcgctg gtgttgagcg tgtcgggatt gttttatttt
     9001 gcccgtttgg tggaacaaaa cctgcgcgaa gtccccaaag gcgtgattga agccgccgcc
     9061 gctatgggtg cgccgccggt tgccatcatc cgcaaagtcc tcttaaacga agcgcgcgcg
     9121 ggcatggttt ccagcattac cgtgcttgcc atcgggcttt tgtcatacag cgcggcggca
     9181 gggatgatag gcggcggcgg cttgggcgac ctcgccatcc gctacggcta ctaccgctac
     9241 caaaccgaag tcatcgtctt catcgtcgcc ctcctcgtgc tgctggtcat cctgattcaa
     9301 agcaccggca acgcgttggc gcggaaactc gacaaacgtt gaacccaaat gccgtctgaa
     9361 cgccaaaacc cccaccgcta tccgaaaaat gctataaaat ccccctgttc gcggcaaatg
     9421 ccgtctgaac gccgaatccg gacggcagga ctccctgccc gtcatttttg tttgaaactg
     9481 ccacaacatc aggagaaaat atgaaaacct tcttcaaaac cctttccgcc gccgcactcg
     9541 cgctcatcct cgccgcctgc ggcggtcaaa aagatagcgc gcccgccgca tccgcttctg
     9601 ccgccgccga caacggcgcg gcgaaaaaag aaatcgtctt cggcacgacc gtcggcgact
     9661 tcggcgatat ggtcaaagaa caaatccaac ccgagctgga gaaaaaaggc tacaccgtca
     9721 aactggtcga gtttaccgac tatgtgcgcc cgaatctggc attggctgag ggcgagttgg
     9781 acatcaacgt cttccaacac aaaccctatc ttgacgactt caaaaaagaa cacaatctgg
     9841 acatcaccga agtcttccaa gtgccgaccg cgcctttggg actgtacccg ggcaagctga
     9901 aatcgctgga agaagtcaaa gacggcagca ccgtatccgc gcccaacgac ccgtccaact
     9961 tcgcccgcgt cttggtgatg ctcgacgaac tgggttggat caaactcaaa gacggcatca
    10021 atccgctgac cgcatccaaa gcggacattg ccgaaaacct gaaaaacatc aaaatcgtcg
    10081 agcttgaagc cgcgcaactg ccgcgtagcc gcgccgacgt ggattttgcc gtcgtcaacg
    10141 gcaactacgc cataagcagc ggcatgaagc tgaccgaagc cctgttccaa gaaccgagct
    10201 ttgcctatgt caactggtct gccgtcaaaa ccgccgacaa agacagccaa tggcttaaag
    10261 acgtaaccga ggcctataac tccgacgcgt tcaaagccta cgcgcacaaa cgcttcgagg
    10321 gctacaaatc ccctgccgca tggaatgaag gcgcagccaa ataaggcagt cgtataaaat
    10381 gatgccgtct gaaccgcatt ccgtgttcag acggcatttt tgtcctttaa tccgccattc
    10441 cctgccattc cgtcgaatcc ggtgtatcga ttccgaacag cgacaaagcg tgtgcaacac
    10501 tgtgcgccac tatgtcgtcc gccgtctgcg gtttgcggta catcgcagga acagggggaa
    10561 acaccacgcc gcccatttcc gttacccgct tcatattgtc caaatgggca aggttcagcg
    10621 gcgtttcgcg caccatcagc accagccgcc gcctttcctt caaaaccaca tccgccgcac
    10681 gcgtcaagag gttgtcgccg aagccgtgcg cgaccgaggc aagtgtccgc atcgaacagg
    10741 gggcgaccag catcccgtcc gttttaaacg taccgctggc aatgcacgcc ccgatattgc
    10801 cgatcggatg cacgaagtcc gccaaggcat atacctcatc tctcgcataa gccgtttccg
    10861 aagcgcgcgc catctccgca cctttcgata ccacaaggtg cgtttcgata tcttgcgcgc
    10921 gtaaaagttc caaagccttc acgccgtatt ggaaaccgct cgccccactg atgccgatta
    10981 tcaaacgccg taccatcatc cgcctttccc ataaaaccgc ctgcaacggc aaaccggcta
    11041 ttatagtgaa aaaacagaaa tccgataaat acggatacaa attgtcggca acacccaata
    11101 tccgataaaa tatccgattt aacatcctat ctgaataggc acgggagggc ggtatggcaa
    11161 aagtaaaagg cggattgggg cgcggtttgg attcgctgct cgccaacggc gcagacaaca
    11221 gcagcggcga ccgattgacc acggttgcgg ttaaagatat ccggcccggc cgctatcagg
    11281 cgcgtgttca aatcgatgac gaagccttgc aggagctggc agattcgatt aaggcgcaag
    11341 gcgtgataca gcccgtcatc gtgcgcgaac acggactgtc ccgatacgaa ctgattgcgg
    11401 gggagcggcg ttggcgtgcc gcacagattg ccggcctgac cgaaatcccc gccgttatca
    11461 aaaccatcag cgacgaaacc gcgttggcaa tgggtttgat cgaaaacctc cagcgcgaaa
    11521 acctcaaccc catcgaagaa gcacaaggct tgaaacgcct tgccgacgag ttcgggctga
    11581 cccacgaaac catcgcccaa gccgtcggta aaagccgaag cgcgatttcc aacagcctgc
    11641 gccttttaag cctgcccgaa cccgtgcagg aaatgcttta ccaacgccgc ctcgaaatgg
    11701 ggcacgcccg cgcactgctg accctgcccg tcgtcgaaca gctcgaattg gcgcaaaagg
    11761 ctgtcaaaaa cggctggtcg gtgcgcgaag tcgaacgccg cagccaagcc gcccttcaaa
    11821 acaaacgtcc cgagcccaaa aaaactgccg ccgccgacat cggccgcctg aatgatttgc
    11881 tgactgaaaa actgggtgtc aacgccgaaa tcaaaaccgc caaccataaa aaaggcagaa
    11941 tcatcctgca tttcgacacg cccgaaacct tcgaccatat cttgaagcaa ttgggcatag
    12001 attaccgacc ttaattttgc gggatatacc gtctgaaata tagagaatag ctttccagat
    12061 tttaagtggg aaatataatt ctattgacat tttttctgct tcacgtaaga atcgttttcc
    12121 tgttttcatt tttaattttc gaggaaatta tgaacacacg catcatcgtt tcggctgcgt
    12181 tcgttgcgtt ggcattagca ggttgcggct caatcaataa tgtaaccgtt tccgaccaga
    12241 aacttcagga acgtgccgcg tttgccttgg gcgtcagccc aaatgccgta aaaatcagca
    12301 accgcaacaa tgaaggcata cgcatcaact ttaccgcaac tgtgggtaag cgcgtgagcc
    12361 aatgctatgt taccagtgta atcagcacaa tcggcgttac cacttccgat gcaatttgtt
    12421 tgggaggcgg aacgcacaaa ggcaaaagtc aatgcaatgc tttgcttaaa gcggcaggcc
    12481 gttgctaatc ctttattcgg aaaaggtcgt ctgaaaatat tttcagacga tcttttattt
    12541 attgagcaaa ttccccaaac tgccgacaat ccgaccgata aactgctaca atttcgcgtt
    12601 gattttttat tatttccacg taaagcaaac aaatcacgta agaacattaa aaatgtaaag
    12661 aaattgcgga caaactcctt aatatcctgt aattatatgt aatgtgtgtt agaatgacac
    12721 ttgtcgccgt tattcaggcg gcgagcgcgg cggattggga tacgttgttt cacaaagatt
    12781 aactgtcgct tgacgtaata aggcttctaa attatattag ccgcgcccca aatccgaggc
    12841 ggaaacattc cctttatgaa gcagattatc atcctacaat ccgccgtatt gtccatatgc
    12901 gccgcagttg cctttgctgt atggggtttt gccggattcc tttcggcggt cggtggcggt
    12961 ttgtcctacc tgcttcccac gtttgttgca gttttacttt taaaactttt caggggaaac
    13021 cccttcctgc aaagcaggat gttcgtcttc ggagagattt taaaagtagt gctgtcgctg
    13081 ttgtccatgc ttgccgtatt tgcaatatgg catcaatcgc tggtgttcgc cccgtttctg
    13141 atggggctgc tcggtgtcag ccatttggtt tttttagtat tgttgagagt gaaagattat
    13201 ggcaggtgaa accattaccg ctgccgacta catcaagcac cacttgcaga gcttgaccag
    13261 tttgtcggat gttactcagg gtcagggact gaaaaacatt gctgattttt cgtttattaa
    13321 ccttgatgcc gtcttttttg ccgtcctgtt gggcgtaatc ggcagctttc tgttgtggcg
    13381 cggtgcgaaa aaagcaacgg caggcgttcc cggacgtttc caggctgccg tggaaatctt
    13441 gtttgagttt gtggacgata tgtgtaagag catcattcac aacgaaaagt ctagaaaagc
    13501 cgtcgcgccg ttgggtctga cgctgtttgt ctggattttt ctgatgaacg cgatggatat
    13561 gctgccggtc gatttgctgc cgatggtatg gcagggcatt accggcaacc atcacgccct
    13621 gctgcgcgtc gtaccgactg ccgatttgaa caccactttg gcactcgctg tcggcgtgtt
    13681 gctgatttgt atctattaca acatcaaaat caaaggattg ggcggctggt ttcacgagtt
    13741 gttcagcgcg ccgttcggtg caaaactcgc acctgcgaac tttctgttga atttggtcga
    13801 gtttctttct aaaaccgtat cccacggtat gcggttgttc ggcaatatgt atgcgggcga
    13861 attggtattc ttgctgatag ccttgcttgg cggtgcttgg gcggcttccg gcagcgtcga
    13921 agtcatggat ccgattctgt ttgtattcca cattattgcc ggtttggcat gggcgatttt
    13981 ccatattttg gtgattaccc tgcaggcgtt tattttcatg gcgttggcgt tcgtctatat
    14041 cggacaggca catgatgcgc actgattttc ctaagtagtt tgtttttgta gtttaacctt
    14101 tgtttcttta aggagtttaa aatgggtttg attgctatcg catgtggttt gatcgttgca
    14161 ttgggtgcat tgggtgcctc tatcggtatc gcaatggtcg gttctaaata tttggagtct
    14221 tctgcccgtc agcccgaact gatcggtccg ctgcaaacca aactgttcct gattgccggt
    14281 ttgattgatg ccgcattctt gattggtgtc gccattgcac tgctgttcgc cttcgttaac
    14341 ccgtttgcag gtgcataatc aggacggatt cgtccgttcc gtacaggcgc agcctgtttg
    14401 attaacccca gtacgaaggt taagtaacgt gaatatcaat gcaacattat tcgctcaaat
    14461 catcgtcttt ttcggtttgg tatggtttac catgaaattc gtgtggccgc cgattgcaaa
    14521 agctttggat gagcgtgccg caaaagtcgc cgaaggcttg gctgccgccg agcgtggtaa
    14581 aagcgatttc gagcaggctg aaaaaaaggt tgcagaactc ttggcagaag ggcgtaatca
    14641 ggtttccgaa atggttgcca acgccgaaaa acgtgccgcc aaaattgtcg aagaagccaa
    14701 agaacaggct tcttccgagg cggcgcgcat tgcagctcag gcaaaggccg atgtggagca
    14761 ggaattgttc cgcgcacgcg aatccctgcg cgagcaggtt gccgtgttgg ctgtcaaagg
    14821 tgccgaatct attttgcgca gcgaagtcga tgcttccaaa cacgcaaaac tgctcgatac
    14881 cctgaaacag gagctgtaat cttatggcag agttctcaac gattgccaga ccttatgcaa
    14941 aggcattgtt cggtctggct caggaaaaaa accaaattga gtcttggttg ggcggactgg
    15001 aaaaacttgc ggcggttgtt caggaaggga aggtggcttc attgattgac cgtcctgaaa
    15061 cgaatgcttc agaaaaagca gatatcctca tcgatttggt cggtttgaaa gacaaggagt
    15121 tgaaaaactt tgttatcgtc ttggccgggc agaaacgttt gtcgatattg ccggaagtat
    15181 atgctcaata tcaagacttg accttatcat tcaaccatat caaatctgcc atcatttaca
    15241 gtgcctatcc gttgaccgac aaacaggtcg gcgagttggc gcaaatgctg aataagcgtt
    15301 tcgacagcga gctgaaaatc tctgtcgaaa tcgaaccgga gctgattggc ggcataaaag
    15361 ttgaagtggg tgatcaggtt ttggatttgt ctgtacaagg caaattgagt gccttgtaca
    15421 cgactatgac gaattaggag agttttcatg cagcttaatc ctgctgaaat tagcgatttg
    15481 attaaagcca agatcgaaaa tctgtctgta aatgccgaag tgcgtactcg tggtacggtg
    15541 atttcggtaa ctgacggtat cgttcgcatc catggtttgt cagatgcaat gcaaggtgag
    15601 atgctcgaat tcccgggtaa cactttcggc ttggcgatga acctggagcg cgactccgtc
    15661 ggcgccgtag tgttgggcga gtacgaacat attaaagaag gcgacacggt tacctgtacc
    15721 ggtcgtatct tggaagtgcc ggtcgggcgc gaactggtcg gacgcgtcgt tgatgcattg
    15781 ggtcggccta tcgacggcaa aggcccgatt aatacaactt tgaccgcccc tatcgaaaaa
    15841 atcgcaccgg gcgtgattgc gcgcaaatcg gttgaccagc cgatgcaaac cggtctgaag
    15901 gcgattgact ctatggttcc tgtcggtcgc ggtcagcgtg agttgattat tggcgaccgt
    15961 cagacaggta aaacggccgt agcattggat gccatcgtca accaaaaagg tacgggtgtt
    16021 atctgtatct atgtcgctat cggtcaaaaa gcatcttcta ttgccaacgt ggtccgcaaa
    16081 ttggaagagc atggcgcgat ggagcatacc atcgtggttg ctgcaactgc atctgaagcg
    16141 gcggcattgc aatatatcgc accttactcc ggttgtacga tgggtgaatt tttccgcgac
    16201 cgcggcgaag atgccttgat tgtttatgac gatttgtcca aacaggctgt ggcttaccgt
    16261 caaatttccc tgcttttgcg ccgtccgccc ggccgcgaag cctatcccgg cgatgtgttc
    16321 tacctgcact cccgtctgtt ggaacgtgca gcacgtgtca atgaacacga agtggagaaa
    16381 ttgaccaacg gcgaagtaaa aggcaaaacc ggttctctga ccgcgttgcc gattatcgaa
    16441 acccaagcgg gcgacgtatc tgctttcgtt ccgactaacg tcatttcgat taccgacggt
    16501 cagattttct tggaaaccga cctcttcaac gccggtatcc gtcctgcaat caatgccggt
    16561 atttccgtat cccgcgtagg cggtgctgca caaaccaaag tgattaaaaa gctgggtggc
    16621 ggtatccgtt tggcgttggc gcaatatcgt gaattggcgg ctttctcgca atttgcatcc
    16681 gatttggatg aagctacgcg caaacaattg gagcatggcg aagtcgtaac cgaactgatg
    16741 aaacagaaac agttcagtac gttgaatacg gctgaaatgg ctttgaccct ttgggcaatc
    16801 aacaacggtt cgtattctga tgttccggtt gccaaagctc tggcttttga atctgaattt
    16861 ttgagctttg tccgtaccca acatccggaa gttttggaag ccgtcaatgc ttcaggtgca
    16921 atgtccgacg agagcgagaa aacgcttgaa gcagccatga aatccttcaa atcttcttac
    16981 gcctatcagg cataaggctt gaagtgaaag gagtctgaaa tggcagtagg aaaagagatt
    17041 ctcaccaaaa tccgcagtgt tcagaatacc caaaagatca ctaaagcgat gcagatggtg
    17101 tcaacctcta aaatgcggaa gactcaggaa cggatgcgtt tggcgcgtcc gtatgccgaa
    17161 aaagtgcgta tggtgatgag ccatcttgcg caaaccaata ccgatcatgg tattccgtta
    17221 ctggaatctc atcgggagat tagacgtgtc ggttttattt tgattacgtc tgataagggt
    17281 ttgtgtggcg gtttgaacgc caacgtgctg aaaaagtttt tggcacaagt tcaagagtat
    17341 cggaatcaag gtattgaaga ggaaatcgta tgccttggca gtaaaggtct gatggcgtgt
    17401 cagagcattg gtctgaatgt ggttgccagt gccgtaaatt tgggcgatac cccaaaaatg
    17461 gaaatgctgc tcggaccttt gacagaactc ttccaacggt atgagaaaca tgaaattgac
    17521 agaatccatc tggtgtattc gggttttgtc aataccatgc gtcaagaacc gagaatggaa
    17581 gtattgctgc ctatcggtga gaacgtgatt ggcgattcag ctcccaaatc accgttcagc
    17641 tgggaatacc gctacgaacc gactgcactt gcagtgttgg aatatctggt tcgccgctat
    17701 ttagagtctg tggtttatca ggcgttgagc gacaatatgg catccgaaca ggcagcccgc
    17761 atggttgcca tgaaagctgc aacagacaat gcaggcaatg ccatcaaaga gttgcgtctg
    17821 gtatataaca aatcgcgtca agctgcgatt accacggaat tgtcagaaat tgtagcaggt
    17881 gcggcggctg tctgatgccg tctgaatcct gtacagaaat taggatacga taatgagcca
    17941 aggcaaaatc gtacaaatta tcggtgcggt tgttgacgtg gaatttccac gcgacatgat
    18001 tccgcgcgtt tacgacgctt tgaaattaga cgaaaacggt ctgactttgg aagtccaaca
    18061 gcttttgggc gacggcgtag tccgtaccat tgcgatgggc agctcggacg gtttgaaacg
    18121 cggcatgact gtgagcaata ccggtgcgcc cattactgtg ccggtaggta aaggtacgtt
    18181 gggacgcatt gtcgatgtat tgggaacgcc tgttgacgag gcaggtccga tcgataccga
    18241 caagagtcgt gccatccacc aagccgctcc taagtttgac gaactgtctt ccacaaccga
    18301 attgctcgaa acgggcatta aagtgattga cttgctgtgt ccgtttgcca aaggcggtaa
    18361 agtaggtctg ttcggcggtg ccggtgtggg taaaaccgtg aacatgatgg aattgatcaa
    18421 caacatcgcc aaagcgcaca gcggcttgtc cgtgttcgca ggcgtgggtg agcgtacccg
    18481 cgaaggtaac gacttctacc acgagatgaa agattccaac gtattggata aagtggcaat
    18541 ggtttacggt cagatgaacg aacctccggg caaccgtttg cgcgtcgcat tgaccggttt
    18601 gaccatggcg gaatacttcc gtgacgaaaa agacgaaaac ggcaaaggcc gcgacgtatt
    18661 gttcttcgtg gacaacatct accgttacac tctagctggt accgaagtat ccgcattgtt
    18721 gggccgtatg ccgtctgcag tgggctacca accgacattg gcagaagaaa tgggtcgttt
    18781 gcaggagcgt attacctcta cccaaaccgg ttctattact tctatccaag ccgtatatgt
    18841 acctgcggac gacttgaccg acccgtctcc ggcaacaacg ttcgcccact tggacgcaac
    18901 cgtcgtattg agccgcgata ttgcctcttt gggtatttac ccggcagtcg atccgcttga
    18961 ttccacttcg cgccaactgg atccgatggt cttgggtcaa gagcactacg acgtggcgcg
    19021 cggcgtacag tccaccctgc agaaatacaa agaattgcgc gacatcatcg ccatcttggg
    19081 tatggacgaa ttgtctgacg aagacaaact gaccgtaatg cgtgcgcgta aaatccaacg
    19141 cttcctgtcc caaccgttcc acgttgccga agtgtttaca ggttctccgg gcaaatatgt
    19201 cgccctgcgc gataccattg ccggcttcaa agccatcttg aacggcgaat acgatcatct
    19261 gcccgagcag gcattctata tggtcggcag cattgaagaa gcggttgaga aagcgaaaac
    19321 cttaaactga ggaggtcggc atgagcatca tgcaagttga ggtggtaagt agcgagcaga
    19381 aaatctattc aggcgaggca acatttatcg ttgttccgac tgtacagggc gaactcggta
    19441 tttatccgcg acacgagccg attatgagtt tggtgcggcc gggggctttg cgtttgaccg
    19501 ttccgggcga ggataaagag gttttggttg ctgtttccgg cggtgttttg gaagtacagc
    19561 ctgataaagt aactgtcttg gcggatgttg ccgtccgcag tgcagagatg gatcaggcac
    19621 gtgcggaaga ggcgaaaaaa gccgcagaag cgggcatttc ccaagctaaa gacgataagg
    19681 ctttggcgga ggcgcataaa gcattggctg ctgcgattgc acagcttaaa actttggact
    19741 atatccgttc gcacaagaaa taaacttgtt tggtttggaa aagcacgacc gtttggtcgt
    19801 gctttttatt ttccggattt aaattaaaga ctgtgtttgg atatggcgtt ggcaggcata
    19861 gcctgcttta aggtattcaa tagggcagcc tgccgcctaa ccgaaacata tagcggatta
    19921 acaaaaatca agacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca
    19981 cttggtgctt gagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgctgta
    20041 ctggtttaaa tttaatccac tatatcatgt atgccgtgtt ttcaaatcag tctgtctttg
    20101 agataaacct atccaaacag caatggaaac agtaaaataa accctttatt ttatcgaatc
    20161 ataagccatg cttaccttcc aacaaatcat cttcaaactg caaaccttct gggcagacaa
    20221 aggctgcacc gtcatccaac ccttcgacat ggaagtcggt gccggcacat cccatcccgc
    20281 cacctgcctg cgcgcgctcg ggcccgagcc ttggtttgcc gcctacgtcc aacccagccg
    20341 ccgccccaaa gacggccgct acggcgacaa ccccaaccgc ctgcaacact attaccaatt
    20401 ccaagtcgcc ctcaaacccg cccccgccaa tatccaagac ctttatctcg actccctgcg
    20461 cgaattgggc atcgacccca aagtgcacga catccgcttc gtcgaagacg actgggaaaa
    20521 ccccaccctc ggcgcatggg gcttgggctg ggaagtttgg ctcaacggca tggaagtaac
    20581 ccagtttacc tatttccaac aagtcggcgg catcgactgc acgcccgtac tcggcgaaat
    20641 cacctacggc atcgaacgct tggcgatgta cctgcaaggc gtggaaaacg tctatgacct
    20701 cgtttgggca aaaacgccgg acggcaacac cgtaagctac ggcgacgtgt accaccaaaa
    20761 cgaagtcgaa caatccacct acaacttcga atacagcgat gccgattggc tgctgcgcca
    20821 gttcaacgac tacgaagcgc aagccaaacg cctgcttgcc gaagaaaacg ccgccctcgc
    20881 cttgcctgcc tacgagctgg ttctcaaagc ggggcatacg ttcaatcttt tagacgcacg
    20941 cggcgcgatt tccgtaaccg agcgggcaac ttatattgga cgtattcgtg cattgagccg
    21001 cgccgtggcg caaaaatatg ttgaaagccg cgaaaaactg ggcttccctt taatgaaggc
    21061 aaatgctgcc taagggggcg acgatttttt acgttcgggc ggcacgataa ggcagccgtc
    21121 cgtaaaccat tatatatttt aggaaaatca tgcctgtcta tttccgcacc ccaagcagga
    21181 aacgcctgca caaaatgaac cgccgccagc ataaaaagct gcatttgggc gagtttcaaa
    21241 cattgatttt cggcgttcaa ggcagtttgc accaagctga ggacgatgcc gaagcgttcg
    21301 accgctttat ggacgagctg attgcgtatc ttgaggtaaa cgactttgga atggcgggcg
    21361 ggggcgattg cggcgatttt tcgtgcctgt ttcaaaaatc cggtgcccga agcttgggcg
    21421 aaaccgagaa aaaagccatt atctcttggc tggcaggccg cgaagatgtc gccactttgc
    21481 acaccacccc gctggttgac ggtttttacg gggatgacgg ctgggaaaat tacagtgaaa
    21541 cctataaaca ggcagattaa ccaaatttaa agaaagccga atgatgaccc aaaccctttt
    21601 aatcgaactc cttaccgaag aactcccgcc caaagcactg aataatctgg gcaaccattt
    21661 tgccgccgcc gttgccgaag gcttggaaaa agcgcaactg gttgacggtg cagccgaatt
    21721 tacggcttat gcctcgccgc gccgtttggc ggttcaagtc aaaaacgtta aagccgttca
    21781 ggccgaccaa aaaatcgtga aaaaaggccc tgccgtggcg aatgccatga aagacggtgc
    21841 gccgaccaag gctttggaag gtttcgcacg cggtgcgggc gcgaaaatcg aagacttgac
    21901 catcgtccac gacggcaaac aggatgtgta cgcctacgaa tacgtccaaa ccggcaaacc
    21961 gctgggcgaa cttttggaag acattatcaa tcaagccgtt aagaaactgc cgattccgaa
    22021 agtgatgcgt tggggcagca gcacgtttac cttcgtgcgc cctgttcacg ggctgattgt
    22081 gctgcacggc ggcgacatcg taaacgtcag cgttttgggt ctgcaaagca gcaatcaaac
    22141 cttgggacac cgcttcttgt ccaacggcga aatcaccatt gaaaacgccg acagctacgc
    22201 cgaacaaatg cgcgggcaag gcaaagtcgt cgcttcgttt gccgagcgca aagccgcgat
    22261 tcagacggca ttggaagggc aggcgcgcag tctgaacgcc gccgtcgccg ccgatgaagc
    22321 attgttggac gaagtaaccg cgctggtcga atggcctgtt gttttggaag ccggttttga
    22381 ggaacatttc ctcgccgtgc cgcaggaatg cttgatttta acgatgcagc aaaaccaaaa
    22441 atactttccg ctgctcgacc aaaacggcaa gctgatgaac cgcttcctgc tggtttccaa
    22501 cctgcaaacc gaagacccgt cgcacatcat ccgaggcaac gaacgcgttt tgcgcgcgcg
    22561 cctgtctgat gccgagttct tctacaaaca agaccaaaaa gcgactttgg aaagccgcct
    22621 gccgaaattg tccagcgtgg tgtaccacaa caaaatcggc tcgcaagccg aacgcatcga
    22681 acgcctgcaa agcattgccg cccacatcgc caaagcattg ggcgcggatg ccgccgctgc
    22741 cgaacgcgcc gcgcgtcttg ccaaagccga cttggtaacc gaaatggttg gcgagttccc
    22801 cgaactgcaa ggcacgatgg gcaaatacta tgcacgtttg gacggcgaaa ccgaagaaat
    22861 cgccgaagcc atcgagcagc actatcagcc gcgttttgcc ggcgacaagc tgcccgaaag
    22921 caaaattgcc gccgccgtgg cactggcgga caagctggaa accctggtcg gcatttgggg
    22981 catcggtctg attccgaccg gcgacaaaga cccctacgcc ctgcgccgcg ccgccttggg
    23041 tattttgcgg atgctgatgc agtacggttt ggacgtgaac gaactgattc agacggcatt
    23101 cgacagcttc cccaaaggtt tgctcaacga aaaaacgccg tctgaaaccg ccgactttat
    23161 gcaggcgcgc cttgccgtgt tgctgcaaaa cgattatccg caagacatcg tcgccgccgt
    23221 actcgccaaa cagccgcgcc gtttggacga tttgaccgcc aaactgcaag ccgttgccgc
    23281 gttcaaacag ctgcccgaag ccgccgcgct cgccgccgcc aacaaacgcg tgcaaaacct
    23341 gctgaaaaaa gccgatgccg cgttgggcgc ggttaacgaa agcctgttgc aacaggacga
    23401 agaaaaagcc ctctttgccg ccgcgcaagg cttgcagccg aaaatcgccg ccgccgtcgc
    23461 cgaaggcaat ttccaaaccg ccttgtccga actggcttcc gtcaaaccgc aagtcgatgc
    23521 attctttgac ggcgtgatgg taatggcgga agatgccgcc gtaaaacaaa accgcctgaa
    23581 cctgctgaac cgcttggcag agcaaatgaa cgcggtagcc gacatcgcgc ttttgggcga
    23641 gtaaccgttg tacagtccaa atgccgtctg aagccttcag acggcatcgt gcctatcggg
    23701 agaataaatt gcagccttta gtcagcgtat tgatttgcgc ctacaacgta gaaaaatatt
    23761 tcgcccaatc attagccgcc gtcgtgaatc agacttggcg caacttggat attttgattg
    23821 ttgatgacgg ctcgacggac ggcacacttg ccattgcccg gcgtttccaa gaacaggacg
    23881 gccgcatccg catcctcgcg cagccgcgca attccggcct gattccctct ttaaacatcg
    23941 ggctggacga attggcaaag tcaggaatgg gggaatatat tgcacgcacc gatgccgacg
    24001 atattgccgc ccccgactgg attgagaaaa tcgtgggcga gatggaaaaa gaccgcagca
    24061 tcatcgcgat gggcgcgtgg ctggaagttt tgtcggaaga aaaggacggc aaccggctgg
    24121 cgcggcatca caggcacggc aaaatttgga aaaagccgac ccgacacgaa gatattgccg
    24181 actttttccc tttcggcaac cccatacaca acaacacgat gattatgagg cgcagcgtca
    24241 ttgacggcgg tttgcgttac aacaccgagc gggattgggc ggaagattac caattttggt
    24301 acgatgtcag caaattgggc aggctggctt attatcccga agccttggtc aaataccgcc
    24361 ttcacgccaa tcaggtttca tccaaataca gcgtccgcca acacgaaatc gcgcaaggca
    24421 tccaaaaaac cgccagaaac gattttttgc agtctatggg ttttaaaacc cggttcgaca
    24481 gccttgaata ccgccaaata aaagcagtag cgtatgaatt gctggagaaa catttgccgg
    24541 aagaagattt tgaacgcgcc cgccggtttt tgtaccaatg cttcaaacgg acggacacgc
    24601 tgcccgccgg cgcgtggctg gattttgcgg cagacggcag gatgcggcgg ctgtttacct
    24661 tgaggcaata cttcggcatt ttgcaccgat tgctgaaaaa ccgttgaaaa acgccgcttt
    24721 atccaacaga caaaaaacag gataaattat gcaaaaccac gttatcagct tagcttccgc
    24781 cgcagaacgc agggcgcaca ttaccgatac cttcggcgtg cgcggcatcc cgtttcagtt
    24841 tttcgacgca ctgatgccgt ctgaaaggct ggaacaggta atggcggaac tcgtacccgg
    24901 tttgtcggcg cacccctatt tgagcggagt ggaaaaagcc tgctttatga gccacgccgt
    24961 attgtggaag caggcattgg acgaaggtct gccgtatatc accgtatttg aggacgacgt
    25021 tttactcggc gaaggcgcgg agaaattcct tgccgaagac gcttggctgc aagaacgctt
    25081 tgaccccgat tccgccttta tcgtccgctt ggaaacgatg tttatgcacg tcctgacctc
    25141 gccctccggc gtggcggact acggcgggcg tgcctttccg ctgttggaaa gcgaacactg
    25201 ggggacggcg ggctatatca tttccaaaaa agcgatacgg tttttcctgg acaggtttgc
    25261 cgccctgccg cccgaagggc tgcaccccgt cgatctgatg atgttcagcg attttttcga
    25321 cagggaagga gtgccggttt gccagctcaa tcccgccttg tgcgcccaag agctgcatta
    25381 tgccaagttt cacgaccaaa acagcgcatt gggcagcctg atcgaacacg accgcctcct
    25441 gaaccgcaaa cagcaatggc gcgattcccc cgccaacaca ttcaaacgcc gcctgatccg
    25501 cgccttgacc aaaatcagca gggaaaggga aaaacgccgg caaaggcgcg aacagttcat
    25561 tgtgcctttc caataaaagg aggagagatg gacatcgtat ttgcagcaga cggcaggatg
    25621 cggcggctgt ttaccttgag gcaatacttc ggcattttgc gccgattgct gaaaaaccgt
    25681 tgaaaaacgc cgctttatcc aacagacaaa aaacaggata aattatgcaa aaccatgtta
    25741 tcagcttagc ttccgccgca gaacgcaggg cgcacattgc cgcaaccttc ggcgcgcgcg
    25801 gcatcccgtt tcagtttttc gacgcactga tgccgtctga agaactgaat cggatgatgg
    25861 cggaattggt gccgggattg gcaaaacagc acctgttgag cgaagtggaa aaagcctgct
    25921 ttatgagcca cgccgtattg tggaaacagg cattggatga aggtctgccc tatgttgccg
    25981 tatttgagga tgatgtcctg cttggcaaag acgcagaaaa gttccttgcc gaagatactt
    26041 ggttggaaga gcgttttgat aaggattctg cctttatcgt tcgtttggaa acgatgtttg
    26101 cgaaagttat tgtcagaccg gataaagtcc tgaattatga gaaccggtca tttcctttgc
    26161 tggagagcga acattggggg acggctggct atatcatttc gcgtgaggcg atgcggtttt
    26221 tcttggaaag gtttgccgtt ttgccggcag agtggattaa agcggtagat tggatgatgt
    26281 ttacttattt ctttgataag gaggggatgc ctgtttatca ggttaatccc gccttatgta
    26341 cccaagaatt gcattatgcc aagttcctca gtaaaaacag tatgttgggt agcgatttgg
    26401 aaaaagatag ggaacaagaa agaagacacc gccgttcgtt gaaggtgatg tttgacttga
    26461 agcgtgcttt gggtaaattc ggtagggaaa agaagaaaag aatggagcgt caaaggcagg
    26521 cggagcttga gaaagcttac ggcaggcggg tcatatcgtt caaatagttt gtgtaaaata
    26581 tagcggatta aaattagaaa tggatacact gtcattcccg cgcaggcggg aatctagtcc
    26641 gttgagtttc agtcatttcc gataaatcac cgcaacgtta aatttctaga ttcccgcctg
    26701 cgcgggaatg gcggtggtgg gatttctgtt tttccgacaa attcctaaaa cttaaagttc
    26761 cgttattccc gcgaaaacag aaaaccaaaa atagaaacct gaaaacagaa acctgaaaac
    26821 agaaacctga aaacagaaac ctaaaattcg tcattcccgc gcagg
//
LOCUS       DAWHXS010000014        26120 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWHXS010000014 DAWHXS010000000
VERSION     DAWHXS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.014"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(140..700)
                     /locus_tag="WIE22_000473"
     CDS             complement(140..700)
                     /locus_tag="WIE22_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ4126615.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINFPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
     gene            829..1020
                     /locus_tag="WIE22_000474"
     CDS             829..1020
                     /locus_tag="WIE22_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126616.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            complement(1216..1410)
                     /locus_tag="WIE22_000475"
     CDS             complement(1216..1410)
                     /locus_tag="WIE22_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ4126617.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(1400..3481)
                     /locus_tag="WIE22_000476"
     CDS             complement(1400..3481)
                     /locus_tag="WIE22_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ4126618.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3957..4337
                     /locus_tag="WIE22_000477"
     CDS             3957..4337
                     /locus_tag="WIE22_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ4126619.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDGGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            4992..6425
                     /locus_tag="WIE22_000478"
     CDS             4992..6425
                     /locus_tag="WIE22_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ4126620.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGTLASKF
                     RNSGQTCVCTNRVYAQSGIYEEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            6534..7322
                     /locus_tag="WIE22_000479"
     CDS             6534..7322
                     /locus_tag="WIE22_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ4126621.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            7690..9246
                     /locus_tag="WIE22_000480"
     CDS             7690..9246
                     /locus_tag="WIE22_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ4126622.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQQEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            9263..10009
                     /gene="surE"
                     /locus_tag="WIE22_000481"
     CDS             9263..10009
                     /gene="surE"
                     /locus_tag="WIE22_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ4126623.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
     gene            10265..11512
                     /locus_tag="WIE22_000482"
     CDS             10265..11512
                     /locus_tag="WIE22_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ4126624.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(11719..12201)
                     /locus_tag="WIE22_000483"
     CDS             complement(11719..12201)
                     /locus_tag="WIE22_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ4126625.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(12354..12515)
                     /locus_tag="WIE22_000484"
     CDS             complement(12354..12515)
                     /locus_tag="WIE22_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126626.1"
                     /translation="MSEQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(12589..13050)
                     /gene="recX"
                     /locus_tag="WIE22_000485"
     CDS             complement(12589..13050)
                     /gene="recX"
                     /locus_tag="WIE22_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ4126627.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(13107..13814)
                     /locus_tag="WIE22_000486"
     CDS             complement(13107..13814)
                     /locus_tag="WIE22_000486"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ4126628.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            14221..15486
                     /locus_tag="WIE22_000487"
     CDS             14221..15486
                     /locus_tag="WIE22_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ4126629.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKIVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(15544..16350)
                     /locus_tag="WIE22_000488"
     CDS             complement(15544..16350)
                     /locus_tag="WIE22_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ4126630.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            16695..16904
                     /locus_tag="WIE22_000489"
     CDS             16695..16904
                     /locus_tag="WIE22_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ4126631.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            16993..18207
                     /locus_tag="WIE22_000490"
     CDS             16993..18207
                     /locus_tag="WIE22_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ4126632.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(18294..20873)
                     /gene="clpB"
                     /locus_tag="WIE22_000491"
     CDS             complement(18294..20873)
                     /gene="clpB"
                     /locus_tag="WIE22_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ4126633.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDASNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            21134..22144
                     /gene="trpS"
                     /locus_tag="WIE22_000492"
     CDS             21134..22144
                     /gene="trpS"
                     /locus_tag="WIE22_000492"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ4126634.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            22150..22491
                     /locus_tag="WIE22_000493"
     CDS             22150..22491
                     /locus_tag="WIE22_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126635.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNNLEAVSGKCMETDDKGGPVGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            22811..23176
                     /locus_tag="WIE22_000494"
     CDS             22811..23176
                     /locus_tag="WIE22_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126636.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(23385..24893)
                     /gene="ppx"
                     /locus_tag="WIE22_000495"
     CDS             complement(23385..24893)
                     /gene="ppx"
                     /locus_tag="WIE22_000495"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ4126637.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(25009..25740)
                     /locus_tag="WIE22_000496"
     CDS             complement(25009..25740)
                     /locus_tag="WIE22_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4126638.1"
                     /translation="MSREINAGRHHFLYGGISGGGVHYWDNKDFSEQSLRLSFGYKNR
                     SVTRSFGIVPFVEQNLLGGSRYNFVGGFNADFSQRLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHNITKEAEQASIRKGLRVGAVKTFDG
                     GLGLLANLRYTRRMFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         5982 a         6512 c         7242 g         6384 t
ORIGIN      
        1 tctaaggtgc tgaagcaccg agtgaatcgg tttcgtacta tttgtactgt ctgcggcttc
       61 gtcgccttgt cctgattttt gttaatccac tataaaattc cggattccca cttttgtgga
      121 aatgatgaga taaacgtgat tatggtttaa acggggcgta tgcccattcc ccgggtttta
      181 aatccaaatc aaacagtttc agtctgccgc ttgccactct gatcagacgc aggcagggat
      241 agcccgcctt ggcggtcatt cgcctgactt ggcggttttt gccctcagaa atggtaattt
      301 caatccaaaa atcgggaacg gttttgcgga cgcgtatcgg cgggatgcgc tcccataacg
      361 aatctgcttc tccgtgtttc aagatgcgga tgcttgccgg acgggtaacg aaaccgccta
      421 agtctatccc ttttcttagg ctttccaatc ggctttcgtc gggtacgccc tccagttgcg
      481 cccagtaggt tttagggtgt ttgaatttgg ggtcggtaat ttgtgcctga agcctgccgt
      541 cgtcggtcag cagcagcagc ccctcgctgt cggtgtcgag ccgtccggcg gggtagaagc
      601 cgggaaaatt gataaagtct ttgaggcttt tgtgtttttc gtgcggtgaa aattggcaga
      661 taacgccata gggtttgttg aaggcgatga ggtttttcat agcttttggg tttgggttga
      721 aatgggcggc ggtatcggga aaatttgttt attgcgaagg gacatccgat aaatggtgct
      781 tccaaagtga aaaggtttga atgcagatta aattttaagg tgcatgaaat ggattttcaa
      841 ttctttgtcg aaacaatccg ccgacaggaa aagaaactga agcatggcgt gtaccggctg
      901 tttgagattt acagttggtt ggaagatgga aaagcaacaa aggcgcgtat ccggtatttt
      961 gtcaaaattg aggtcgataa acagatattg cgaaggattc atcgtggtat agcggattaa
     1021 caaaaaccgg tacgtcgttg tctcgccttg gctcaaagag aacgattctc taaggtgctg
     1081 aagcaccaag tgaatcggtt tcgtactatt tgtactgtct gcggcttcgt cgccttgtcc
     1141 tgatttttgt taatccacta tattataaaa attccgtctg aagcggcgtt cagacggaat
     1201 ttgtttcaag caggcttaac agcagcgtcc gccgtttgcg ccgcagcggc gtttgcggca
     1261 atagtcttga aactgcagct tggtcatcac gggggcgttg ggattatgtt tgcgctgctg
     1321 tgcaacgtag ttttcataat cgggcacgcc tgccatcaag tttgccgtca gcttgatggt
     1381 tttccaccaa gacgcgagct tatgcttcac tttgtgcctc cggctgtttg ccgtcgcggt
     1441 acaccgccgg gatttctttg gcggtcggcc agccgacttt gcgtgctttg agggcggtac
     1501 gcaaaccgta cgcggcgaca atcacgacaa ccgacaagaa gaggatggtc agaccggcat
     1561 taatcttgtc gttgaagatg atttgcgcca tttcgccgat gtctttggca ggcgcaagga
     1621 cttcgttttt agccaatgcg tcgctgtatt tgccggcgtg ggcaaggaag ctgatgcgcg
     1681 ggtcgctgtg gaacagtttt tgcaggccgg cgtagcaggt tacgaacagt acgccgacgg
     1741 cgggaacgag taccacccag acataacggt cgcgtttcat cttaatcagc accacggcgc
     1801 acataatcaa ggctacgcct gccagcattt ggttggcgat gccgaacaaa ggccagagcg
     1861 agttgatgcc gcccagcggg tcggttacgc cggtgtagag aaagtagccc cacaatgcca
     1921 cggcgaagaa ggtcgcaatc aggttggcgg ggatggagtc ggtgttgccg aaaggtttgt
     1981 agaagatgct gcccaagtct tgaatcataa aacgtgcgac gcgcgtaccg gcatcgacgg
     2041 cggtcaggat gaacaaggct tcaaacaata gggcgaagtg ataccagaac gccatcatcg
     2101 cctcgcccgg aatcaggcgg ctcataatgt gcgccatacc gactgcgagg gtgggcgcac
     2161 cgcctgcacg ggaaaggatg gtgttttcgc ccacttcctt agcggtgtgc aacagggttg
     2221 cagcatcgac agggaattgc agcttggtgg taatcacttc ggcggcggta ttggcatccg
     2281 taccgatcag ggcggcaggg ctgttcatgg cgaagtacac gccgggatca agcgatgcgg
     2341 cggcggcaag tgccataatg gctacgaaac tttccatcaa cataccgccg taaccaatca
     2401 tacggacgtg ggtttcgttt tccagcattt tcggcgtggt gccggaggaa atcagcgcgt
     2461 ggaagcccga aaccgcaccg caggcgatgg tgatgaagag gaacgggaac aatgcgcctg
     2521 agaataccgg gccggaaccg tcgataaagt gggttacggc aggcatttgc aaagtgggat
     2581 tgacgatgac gatgcccaaa gccaaggccg caatcgtacc gattttcagg aaggtggaga
     2641 gatagtcgcg cggagtgagc agcagccata cgggcaatac ggaggcgaca aagccgtaaa
     2701 tcataatcgc ccaagtgagc tggatgccgt caaggtcgaa ccaatgtccg atggaacttt
     2761 tcgccacatc ttcgccgtaa attaccgcca gcatcagcaa aataaagccg acgatggaaa
     2821 tctcgccgat tttgcccgga cggatatagc gcgtgtaaat acccataaac agggcaatcg
     2881 gcatagtggc ggcgatggta aacgtacccc aagggctgtg aaccagtgct tttacgacaa
     2941 tcaacgccaa caccgccata atgatgacca taatcatcaa aataccgatg gaggcaatca
     3001 cgccggggac agtgccgagt tcctgtttca caatatcgcc caaagactta ccgtcgcggc
     3061 gcatagagac gaacaagacc atcatatcct gtaccgcgcc ggcaaacacc acgccgaaga
     3121 taatccacaa agtaccgggc aggtaaccca tttgcgccgc caaaaccggg ccgaccaaag
     3181 ggcccgcgcc cgcaatcgcg gcaaagtggt gtccgaacaa tacgcctttg tgcgtcggaa
     3241 cgtagtccaa gccgtcgttg tggcgttctg ccggagtcag gcggttagga tcgagccgca
     3301 ttacgcggtt ggcgatgtag aggctgtaaa aacggtaggc gatgcagtaa acggatatgg
     3361 cggcggtgac catccatacc gcgctgacct gttcgcctcg gctgagggcc agagtggtaa
     3421 aggatgctaa gccgaccagt accactatgc cccaaatgag gaaggttttg agtgatttca
     3481 tcgaataaat ccttatctca cactgtcgga atatgcctga acggcgggaa acggatgacc
     3541 gcctgccggc acgggcacaa accggagtct gaatgattga cgaagtatga aacagtgcgc
     3601 ttgtcgggat ttgtgcctta tgcccgcatc aaacagtgca gggatgcggg cgcacaaaac
     3661 gttaaacgcc gaataggatt ttaacgcaaa ttagcacact gatagcggtt tttacttgga
     3721 atttggaaaa aatttacatt ccttcgggcg gacaggcggg tttggacggc atcgtcaggc
     3781 aaaaggcggt gccggaagac gggtaaagaa ggggcgcgca atccggatta ttgattcatc
     3841 gcgggtaaat tccggttatc gggcttgtgt gtttgcgcgt ccgtttatag tatggcgttg
     3901 ccgcagcttg gaatcagggc ggttgtttca tatcttattt ttattgggga gcttttatga
     3961 atatcaggtt tttcgcgctg accgtaccgg ttttgtcttt ggcggcctgt gccgtgccgg
     4021 aagcgtatga tggcggcgga cgcgggcata tgccgcctgt tcaaaaccaa gccggcacgg
     4081 acgattttcg ggcgttttcc tgcgagaacg gtttgtctgt gcacgtccgc cgtttggacg
     4141 gcggcagaat cgcgttgcgg ttggacggca ggcgtgccgt cctctcttcc gacgttgccg
     4201 catccggcga acgctatacc gccgaacacg gtttgttcgg aaacgcaacc gagtggcacc
     4261 agaaaggcgg cgaagccttt ttcggcttta ccgatgccta cggcaattcg gtcgaaactt
     4321 cctgccgcgc ccgctaaacg gggcagcctg tttgaaccgc cgtccggatt tttcgggcgg
     4381 ttttttgtgt cggatttgtt ttggctgtcg gctggtttcg gcggttttcg ggcgtggcgg
     4441 attatttttc gtttcgttcc gggtttatcc tatcggcagc ggctcaatgc caactttaaa
     4501 cctgctccga tttcttcagg gctgttatcc aatgataaaa tatagtggat taacaaaaat
     4561 caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt cacttggtgc
     4621 ttaagcacct tagagaatcg ttctctttga gctaaggcaa ggcaacgctg tactggtttt
     4681 tgttaatcca ctataatgtt tggatttttg ccatactcta ccacacgttg caactgcaat
     4741 ctttgttcct tatatattat taaagggctg cattactttt ctttctaaat taccattgta
     4801 taaaaagtat ttttcttaaa cgacagggat tgcgcccgct ccatattcag acaccgcccg
     4861 gatgttgcgc tgcccgatcg gatgcttcag acggcatcgg aagggtttgc agttttggaa
     4921 tatgaagatg ataatgtccg cgagattgac ggcatttgaa agcggttatt ccgcataccg
     4981 gaggatacga aatgaacgaa tattcccaat taatcaagca tcccgatatt tcccttcccc
     5041 cggtttcaga cggcatcaaa gtcgataatc cggcaacggg cgagactttg gcgtttgtcc
     5101 gcaagacgga ttcggacaag ctgaaaaacc tgattcaaaa ggcggcggca gcgcaaaaat
     5161 tatgggcggc aaaaaccgcg ttggaacgcg ccgatgtgtt gtggcgttgg tattttttga
     5221 taaaggaaaa caaagaagca ctggcgcgtc tcatgacgat ggagcagggc aaaggcctga
     5281 ccgaggcgcg cggcgaaatc gattatgcgg cttcgtttgt gcgctggttt gccgaagagg
     5341 cgcggcggat tgacggcgat gtgctgacga gtgtgaaagc gtcgcaaaaa ctggtcgtgt
     5401 tgaaacagcc tgtcggtgtt accgccgcga ttacgccgtg gaactttccg tccgcgatga
     5461 ttgcgcgcaa ggctgcgcca gctttggcag tgggttgcgc gatgatagtc aaacccgcgt
     5521 cgctcacgcc tttgagcgcg tatgcgttgg ccgtgttggc ttacgaagcg ggcgtgccgc
     5581 aggatttgct gcctgttgtc agcggcagtg cttcggaaat cggccgtgaa tttgccacaa
     5641 atccgattat ccgcaaaatc agtttcaccg gctctaccga agtcggcgcg aaaatttttg
     5701 ccgacagcgc ggcagacatt aaaaaactca gcttggaact gggcggcaac gcgccgttta
     5761 tcgtgtttga cgatgccgat ttggacaaag ctgtcgaagg tacacttgcc agcaagttcc
     5821 gtaatagcgg tcagacctgc gtctgcacca accgcgttta cgctcaatcc ggcatttacg
     5881 aggaattttg ccgcaaattg agtgaaaaag cagccgcgct caaattgggc aacggcttgg
     5941 aggatggcgt aaaccaaggg ccgctgattg aggaaaaagc ggtagaaaaa gtcgagcagc
     6001 acatcgccga cgcgctctca aaaggcgcgg tctgtctgac cggcggcaaa cgcagcgcgt
     6061 tgggcggaac gttttttgag ccgaccgtct taagcggtgt aacggcgcaa atggcggtag
     6121 ctcgcgagga aactttcggg cctttgtgtc cggtattccg ttttgaaacc gaagccgagg
     6181 tcatcgaggc tgcgaacaat acggaatacg gtttggcagc ttaccttttc accgccgaca
     6241 ccgcccgcca atggcgcgtc ggcgaagcct tggaatacgg catggtcggc atcaatacgg
     6301 gcttaatcag caatgaagcg gcaccgttcg gcggcgtgaa acgcagcggt ttgggacgtg
     6361 aaggcagcaa atacggtgcg gacgaatatc tagaattgaa atatttgtgt atggatgtcg
     6421 ggtgagggat gccatccgaa cggcagttcg ccggactgtg ccgcatttta agcagtctct
     6481 atctgttgta caatgcgccc tgtttttacg gttatttttg atttgaacaa gatatgatgg
     6541 aaaacggaaa aacagtcccg agatggcgtt ttgccttgaa aagtgcgggc tggcacctct
     6601 taatcagcct atcggttgca ggactggcgg cattgctggt ttttaaggtt tggtatcctt
     6661 atccttatgc cgagctgacg ggagggctgt cgctttatca gctggtggtg gctgtcgata
     6721 tcgtatgcgg cccgctgctg actttaattt tggcaagccc gaagaaaaag acaaaggcac
     6781 gcatggtcga tttttccatg gtcggcatca tccagctggc ggctttggcg tacggtctgc
     6841 acagcgtttc gctggcgcgt cctgtggtgg aagcgtttga acaggatcgt atgaccattg
     6901 ttacggcggc ggaagtcgtg gtcgaagatt tgcacaaagc ccccgaaggg ctgcaaagcc
     6961 tgtcgtggtt cggcatccgc cgcattgcat tgaaagaacc tgaggatgcg gatgagaaga
     7021 acaagacgct ggatttgtcc ctgaaaggta tcgagccgag tatgcgtccc gaccggtggc
     7081 tgccgtattc cgacaaggaa gcagaagaaa tccgcaaaca tctgaaaccg ctgaaagtct
     7141 tggcggatgc gagaaaaacg acggttgcgg acattctgaa acaggcaggt ctcgccgaag
     7201 gggaggagct gtattacctg ccgtttacca gcagcaggca gaaagagtgg atagtcatta
     7261 ccgataaaga gggcaacacc aaaggctacg cgccgataga cggcttcatc atcacccctt
     7321 aagcgttggg actccgtccg cactcgaaca tccgttcttc gcggcggtag aatcagactg
     7381 tatttgagag aggcgcaacc cctcaatcag ggcaacacat aggggaaggc tttatgtgcc
     7441 gtccgtgtgt tgaaacattt caaaataagc tcatgccgtc cgaacatcct ttcagacggt
     7501 atggcgtttt gccattgccc cgataagctg ttaaactatt taaattattt cgaccgaagg
     7561 tacacgcaca tgcaagaccc cgagcagagc agcaagcccg cccgccgttt tttgtgcgta
     7621 ccgtctgttc aggcagcttg agcgcttcag gctgcctttt cgcgtttgaa cttgaggaat
     7681 acgaaaatca tggatttcag ttggttggca gaaccgcata cctggatagg ttttgccacg
     7741 cttttggtgt tggaagtcgt attggggata gacaatcttg tctttgtggc gattttggca
     7801 aacaaggtcc agcccgcacg gcgcgaccgc gcacggatta tcgggctggg gctggcagtc
     7861 gtcatccgca tcattatgct tgcttttatg gcgcacatca tcacgctgac cgagccgctg
     7921 ttccaaatcg gcggcctcgc cgtttccggc aaggacatga ttatgctcgc gggcggtatt
     7981 ttcctgcttt acaaagccac caccgaactg catgaacgcc tcgaagggca caaccgtttt
     8041 accgttgccg acagccaaaa aaaacacgcg ccgttttggg gcgtggtcgc gcaaatcctg
     8101 atactggatg ccgtgttttc catcgattcg gtcattactg cggtggcgat ggtcgatcat
     8161 atcgtcgtgg cgatgggtgc ggtcgtcgtc gcaatggctg taatgatttc tgccagcaaa
     8221 ctcttgaccg aatttgtcga cagacaccct accgtcgtga tgctctgcct tggttttttg
     8281 ttgatgatcg gtttcagcct gattgccgaa gccttccatt tccacattcc caaaggctac
     8341 ctctacgccg ccatcggctt ctcgatttta atcgaactgt ttaaccagat ttcccagcgc
     8401 aacagccgca aaaacgacta catcggcagc tcgtggcgca aacggactgc tgaaaatgtc
     8461 ttgggtatga tgggcatacg cgaaagcgtg cttgccgatg cgggcggcga atctggggac
     8521 gacgcgcatt ttgaagaaaa cgaaaaatcg atgatacgca gtgtgctgac gcttgccgaa
     8581 cgcccgatta tgggggtgat gatcccacgc cgcgacatcg aacggctgga catttcccaa
     8641 agccgcgaag aacagtatgc ccaactgcaa aacacgcctt acagccgcct gctcgttgtc
     8701 ggaaaggcgg gcgtggacga acctttgggc tacatcaaca aaaaagacct gctgtcccaa
     8761 ctgctggaaa caggcggtct cgacattcag acggcattgc gccagccgct cgttttgccc
     8821 gacagcacta ccgcgctggg cgcaatcgaa ctcttccgcc aaagcagcgc ggattatgct
     8881 ttggtggtgg acgagttcgg cgcggtattg ggcatggtaa ccatgaaaga cctgctcgaa
     8941 accatcgcag gcgagttccc cgaagaattt gagcgcgaag aagaaccagc cgttcagggg
     9001 aatcccgatg aaagcctgac ggtggaaggc gcgttggaat atgtggaact cgcaccgcaa
     9061 ctcaacctgc cgcagcagca ggaagatgcc gatttccata cggttgccgg gctgattatg
     9121 gaagaattgc aaaccatccc cgatgtcggc gattttgccg atttccacgg ctggcggttt
     9181 gaagtggtcg aaaaagaagg gcagcgcatc gagcgggtca aaatcaccaa attgcccgaa
     9241 gaataagcat tcaggataga aaatgaacgt tttgatttcc aacgacgacg gctacctctc
     9301 cgaaggtatt gccgttttgg cgcgcgttac ggcggaattt gccaacgtca gggtggttgc
     9361 gcccgaacgc gacaggagcg gggtcagcaa ttcgctgacg ctggaacgcc ctttgcagtt
     9421 gaaacaggcg caaaacgggt tctactatgt caacggcacg ccgaccgact gtatccacat
     9481 cgggcagtct gtattttcgg attttcaggc cgattttgtc ttttcgggca tcaaccgggg
     9541 cgcgaatatg ggggacgaca cgctttattc ggggacggtt gcggcggcaa ccgaagccta
     9601 ccttatgggc atacccgctg tggcgttttc cttaaacgac gcttccggac gctattgggc
     9661 gaccgcagaa caggcactgt ggacattgtt ggcgcatttt ttcaaaaacc ccccgcagtc
     9721 ccctattttg tggaacatca atatccccgc cgtcgagccg gaagatgtgc ggggcattaa
     9781 aatcgcccgt ttgggcagga ggcatcacgg tcagaacgtc atccccgcgc gcaatccgcg
     9841 cggcgaacag atttattgga taggcccggt aggcagggtt tccgacaaag aagagggaac
     9901 ggatttcggt gaatgcggcg caggtttcat taccgtaacg ccgctgcaaa tcgacctgac
     9961 cgcctatccg gacatggcgg aaacagcggt gttctggcat gcggactgac cgtttcatca
    10021 aatatagtgg attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga
    10081 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctgcggc
    10141 ttcgtcgcct tgtcctgatt tttgttaatc cactataccg tcgggctttg cgcttgagaa
    10201 gaacgcgcat ttttattatg atataccgca ttcaggctat atatcggaac atgaaggtta
    10261 tcttatgttg aaacaaacga cacttttggc ggcttgcgcc tcctttgccg cactgttggg
    10321 cggttgtgct acccagcagc ctgcccccgt cattgcaggc aattcaggta tgcaggacgc
    10381 gccgtcttcg gcagtttaca acaaccccta tggagcaacg ccgtacagcc cggctcctgc
    10441 cggcgatgcg ccttatgtgc cgccggtgca aagcgcgccg gtttatacgc ctcctgctta
    10501 tgttccgccg tctgcacctg ccgtttcggg tacatacgtt ccttcttacg cacccgtcga
    10561 catcaacgcg gcgacgcata ctattgtgcg cggcgacacg gtgtacaaca tttccaaacg
    10621 ctaccatatc tctcaagacg attttcgtgc gtggaacggc atgaccgaca atacgttgag
    10681 catcggtcag attgttaaag tcaaaccggc aggatatgcc gcaccgaaaa ccgcagccgt
    10741 aaaaagcagg cccgccgtac cggctgccgc gcaaccgccc gtacagtccg cacccgtcga
    10801 cattaacgcg gcgacgcata ctattgtgcg cggcgacacg gtgtacaaca tttccaaacg
    10861 ctaccatatc tctcaagacg atttccgtgc gtggaacggc atgaccgaca atatgttgag
    10921 catcggtcag attgttaaag tcaaaccggc aggatatgcc gcaccgaaaa ccgcagccgt
    10981 agaaagcagg cccgccgtac cggctgccgt gcaaacccct gtgaaacccg ccgcgcaacc
    11041 gcctgtgcag tccgcgccgc aacctgccgc gcccgctgcg gaaaataaag cggttcccgc
    11101 gcccgccccg caatctcctg ccgcttcgcc ttccggaacg cgttcggtcg gcggcattgt
    11161 ttggcagcgt ccgacgcaag gtaaagtggt tgccgatttc ggcggcaaca acaagggtgt
    11221 cgatattgcc ggtaatgcgg gacagcccgt tttggcggcg gctgacggca aagtggttta
    11281 tgcaggttcc ggtttgaggg gatacggcaa tctggtcatc atccagcata attcttcctt
    11341 cctgaccgca tacgggcaca accaaaaatt gctggtcggc gaaggccagc aggtcaaacg
    11401 cgggcagcag gtcgctttga tgggcaatac cgacgcttcc agaacgcagc ttcatttcga
    11461 ggtgcggcaa aacggcaaac cggttaaccc gaacagctat atcgcgttct gactttctgc
    11521 ctgaaatcaa gttggaaatg ccgtctgaaa ggttttcaga cggcattttt gtttttggcg
    11581 gctgtgtttt caaatcattc ggaagggacg gggcatataa atttgtttga ctgtgtttgg
    11641 gtatttgctt gatttcatcc gtttgcagta gaaacggatg gcgcgatgtg cctgtctttc
    11701 agacggcata gtccgccgtc agcagccgca ggacatgtct tcagaggctt gcaggctgat
    11761 gtctctgcgt tttttggctt tttcgtagcg gcagcgttgg cggtcggtca ggatttccag
    11821 cggagggaca gggacgggtt tgccgtcttt gaccgcaacc atcgtgaagt agcagctgtt
    11881 ggtgtggcgg atttcgccgg tgcggatgtt ttgtgcttcg acacggatgc cgatttccat
    11941 cgaggtgcgt cccgtgtagt tgacagcggc gtaaaaggta accaaatcgc cgatgtggat
    12001 gggttcttta aacaggactt tgtcaaccga cagggtaacg caataattgc cgctgtaacg
    12061 gctggcgcag gaataggcaa cctggtcgag caggagcagg agttcgccgc cgtgtacgtt
    12121 gccgctgaaa ttggcggtgt ccggcatcat cagttcggac ataatgagtt cgtgggaagg
    12181 cagttggcgt tgttgtgtca tagtgtacct gttttaagaa agggtatttt gaaatgggtg
    12241 tggaaaaatt ttagggaatg ggaaagtgga attcaagcta aaaaaagcta tgatgggata
    12301 gcctgccgaa atgggctgcc gatgccgtct gaaggtttca gacggcattt tgtttaggaa
    12361 agccaaaaac ggaagaccag gaaaaacacg agtgccaata cgcccgccca aacgacgagc
    12421 atgaatccgg caacagcccg tcccatacgg taaaccgggt ttttgcgttc gtcttcgatg
    12481 ggggaggtgt ggtgtttttc gggttgttcg gacattttgt tctcaatcgg aaaagttgga
    12541 aacgggcgta aggttatgtt gccgtgcaaa aagattcaag aaggaaggtc agcagccgtc
    12601 ttcccaattt tcgtcccaag cgtgtttcaa tgccgtctga acggtatcgg catcgaatcc
    12661 gcgataggcg aggaaacgcg cctgtttttg tttttcttta aggtcggcgg cggggtgttt
    12721 gaatttttta cgcaacacgt ctatggcggc ctgtttttcg cttgagcggt cgggaagcag
    12781 gttgcggctg gtttcttcat ctatgccctg ttgcgccaaa gcctgtttca gcctcaatga
    12841 accgtgtttg cggcttttgc tgcggatata ggcttcggca tagcgcaaat ccgactgcca
    12901 gttgcgttcg gcaaattcgt ttaacacgtt ttccaactct tcttcgcttt cggcgtgcgg
    12961 tgcaagtttg cgtttcagac cgatgcggct gacttcttgg cgcgagagga tgtccatcgc
    13021 gcgggcgcgt agggattttt gcggtttcat ggcggcagtt tcggcgtgcg gcaggtttca
    13081 gacggcatag cgtgccgtcc gaatgcctac tcttctttat ttttctgcgg ttgcaggttt
    13141 tcgtaaattt cgggcagcga gccgataatc cagtcggggc gggtcgcatc gtcttgcgag
    13201 agcagcgtca tatcgccgta accgaaggta acgccgacgc tcaggcagcc ggcggctttg
    13261 gcggcgatga tgtcgttgcg cgagtcgccg accataacca tgtttgcaac atcgataccc
    13321 aaaacttcgg cggcgtgccg cagcggcagg gggctgggtt ttttttcggg caggctgtcg
    13381 ccgccgagta tcaggctgaa gtagtcggca agtccgagct gtttcaacag ttcggcggca
    13441 aggatttcgt ttttattggt aatgactgcc agcgggatgc ccaaagattt gagcaatgcc
    13501 agcccggctt cggtttcggg ataggggcgg gtgaagacgc tcaaatggtc gcggtagtat
    13561 ttcatatagg atacgaaacc tttttcccac agttcggaat cggcttcgcg gtcgcggtcg
    13621 ttggagagga cgcggtgaac cagtttgccg atgccgtcgc ccacatagct ttcgaccact
    13681 ttggcgggca gcggtttcat accgagttgt tccaacatcg cttctgcggc ggcggcaagg
    13741 tcggggacgg aatcgcacag tgtgccgtcc aaatcgaagg cgacggcttg gacgtgttcg
    13801 atggctgcat tcataatctg atttcctgtc gggttgaaag ggtttatttt aacatcgttt
    13861 gcacagggct tctaatgtat agattgttaa caatcgcaac aggttttgca ggcggtgttt
    13921 tcggatttcg gggcggaatc gggcaagggt ttgaaatccc ggcgatgttc caattaaatt
    13981 tttaaaaaat aaaaggatgc ctatatcttt atttaatttg acatttattt ttcggatgcg
    14041 cgcgctgctt tttcaggcgg agtccgggct gattccgtgc ggcttgaaaa ataaaatcat
    14101 atatagatta atcaaatcgg gcttatgccg actacacgct attacatttt gtcgggaaat
    14161 tggttatagt aatgagcgaa atgcacgccc ctgaaaatct ttttgtgaaa aggaagcaaa
    14221 atgtctgaag cccttgcaaa agaaaccctc aatccgtttg aaatcgcgcg caaacaggtc
    14281 aaaaccgcct gcgaccggct gaaaaccgat ccggccgttt atgaaatcct gaagagtccg
    14341 acccgcgtgt tggaagtcaa tttccccgtc aaactcgacg acgacacggt caaaaccttc
    14401 acgggctacc gttcgcaaca caacaacgcc gtcggcccct ataaaggcgg cgtgcgcttt
    14461 catcctggtg tgaatctgga cgaagtcaaa gccctgtcga tttggatgac catcaaatgc
    14521 tgcgttgcgg gtattcctta tggcggcggc aaaggcggca ttaccttgga tccgcgcgat
    14581 tattccgagg cggaactgga acgcatcgcc cgcgcttatt ccgaagccat ttctccgctg
    14641 attggcgaga aaatcgatat tcccgcacct gatgtgaaca ccaacggcaa aatcatgtct
    14701 tggatggttg atgcctatga aaacgtggtg aaacattctg caccgggcgt atttaccggc
    14761 aaaccggttg agttcggcgg ctctttggcg cgtaccgaag ccaccggtta cggcgtgaat
    14821 ctcgctgccg tccaagcttt ggaaaaactg ggcaaagacg tgaagggtgc cacttacgcc
    14881 attcaaggtt tcggtaatgt cggctaccac acaggttatt acgcgcatca atccggtgcg
    14941 aaaatcgttg ccgtttctac tgttgacgtt gccatctaca atgaaaatgg cttggatatg
    15001 gaagcaatca tcaaagaatt ccaaaccaaa ggtttcatca ccaatgaagc cggctacggt
    15061 aaagaaatca gcaatgccga acttttggct ctggaagtgg acgtacttgc cccttgcgca
    15121 ctggaaaacc aactgacttc tgaaaatgcc ggtaaagtcc gcgcgacaat cgtggtcgaa
    15181 ggcgcaaatg gcccgactac tccagaggca gacgttatcc tgcgtcaaaa cggcgtattg
    15241 gtcgtgcctg atattctggc gaactgcggc ggcgtggtcg tttcctattt cgagtgggtg
    15301 caaaacctgc aaggctatta ttgggagttt gacgaagttc aagagaaaga aaccgtcgtc
    15361 ctgcgccgag cgttccgcga tatttggaac ttggctcaag aatacgatgt cgatttgcga
    15421 accgcgtctt atatgatgag tatccgccgt gttgaaaaag cgatgaaact gcgcggttgg
    15481 tattgatgtt tgaacaaaaa atgccgtctg aagccttcag acggcatttt tgtgcgggcg
    15541 ggattaatgg tgcgaatggc cgatttggaa ggttaaagtc gagtagttcg cctgtttttg
    15601 gcacacgctt tgatcgggga agtcggcttt gtgttcgaca ttggctttcc agaagccttg
    15661 gcgcaagggg atgatgtcca cttcgccttt gtcgtctgtg ctgtcggaga aagcctgtgc
    15721 ttcggttttg tgcgttttgc tgcggtcgct ggtgtcgaag ccgtcaaagg tggcggtaac
    15781 ggtggcattg ggcagcggtt cgccacggaa cagaacgcgg actttgaagc gttcgcctac
    15841 gtgaatgttg gcgggattgt ccagcgggac gatttccaag ttttgtccga ccggtttggt
    15901 gatgatggcg gtgtccgcgc tttcgtgtcc gacgttgacg atgtttttgc cgaacatacg
    15961 ggtttgttcg caatagcttg cgtcaggcat ttctttgatg cccgcctgtt tccagcctgc
    16021 tttgtttttt gaccagaaag taggctgata ttcggcgatg acgaggtaac tgccgtcctt
    16081 aacgggacgg ttgcttcggt actggtagtt gtatgtgccg cgttgaatca tgttttcctt
    16141 gcctttttcg gtaaccagct gcatcggttt gctgaagatg tgcaggcggt ctttggcgat
    16201 gggttcgagt tcgggaaatt cgccgtagcc caagtcggct ttaaggtatt cgccgccgtg
    16261 cgtgtgggcg gtttcgaccc agacgcggtg ggcgtgggcg gatgtggcaa atagggcgga
    16321 aacggcgagc agtgtcaatg ttttcttcat ggtgcagcct ttgtctcgtg tgggttaaag
    16381 attttgttat gttataacaa aataggtatc gtgtgaagac ggatttgctg tctgaaaact
    16441 gtctttttgt atagtggatt aacaaaaacc agtacggcgt tggctcgcct tagctcaaag
    16501 agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtgcta tttgtactgt
    16561 cttcggcttc gccgccttgt cctgattttt gttaatccgc tatatcgatt tgaatttttc
    16621 agaaaatgaa gcggacgttt gggcggaggc aacttgtttg ataagatagc aatatttcaa
    16681 aacggagaaa gatcatgcct tatgtcaata ttaaagtaac cggcggcaag gaagcaccga
    16741 ctgccgcgca aaaagcggaa ctgatcggcg gcgtaaccga attgctggca cgcgtgctgg
    16801 gtaaaaatcc cgaaacaacg gttgtcgtga ttgacgaagt ggataccgat aactggggaa
    16861 taggcggcaa aagcgtcagc gaacggcgca aagagggcag gtaaaaagcc tgaaaatctc
    16921 ggtttgatgc tttaaattcc gcgtgaaaaa gagtacattc ccacccattg cccaaaattt
    16981 acggaacaca tcatggataa atttcccaag tctgcaaagc tcgatcacgt ctgttacgac
    17041 atacgcgggc cggttcacaa aaaagccctt cagttggaag aggagggcaa taaaatcctt
    17101 aaactcaata tcggcaaccc tgcgccgttc ggctttgaag cccctgatga aatcttggtc
    17161 gatgtcatcc gcaacctgcc gacttcgcaa ggctattgcg attccaaagg gctgtattcc
    17221 gcccgcaaag ccattgttca ctactatcag accaaaggtt tgcgcgatat tacggttgat
    17281 gatgtctata tcggcaacgg cgtgtccgag ctgattacga tgtctatgca ggcattgctc
    17341 aacgacggcg acgaaatcct gattcccgcg cccgactacc ctttgtggac ggcggcggca
    17401 acgcttgcgg gcggtacggt acgccattat ctgtgcgacg aagaaaacgg ctggttcccc
    17461 aaccttgccg atatggaagc caaaatcacg cccaaaacta aagccatcgt cgtcatcaat
    17521 cccaataatc cgacaggcgc ggtgtacagc agggaaatcc tgttggaaat cgccgaactg
    17581 gcgcgcaagc acggtttgat tattttcgcc gacgagattt acgataaaat cctttatgac
    17641 ggcgcggttc accaccacat cgccgcgctc gcccccgacc ttttgacggt aacgttcaac
    17701 ggtttgtcca aagcctaccg tgtagccgga ttccgccaag gctggatggt gctcaacggg
    17761 ccgaaacatc atgcaaaagg ttacatcgag ggtttggata tgctctcgtc catgcgcttg
    17821 tgtgcgaata cgccgatgca gcatgcgatt cagacggcat tgggcggtta tcagagcatt
    17881 aacgaattca ttttgcccgg cggacggctt ttggaacagc gtaacagggc gtgggaattg
    17941 gtcaatcaga tacccggcgt atcctgcgtc aaaccgatgg gcgcgatgta tatgttccca
    18001 aaaatcgata ccgaaatgta ccgtatccac gatgacatga aattcgttta cgatttgctg
    18061 gtgcgcgaaa aagtcttgct ggtgcaggga acggggttta attggatcaa gcccgaccat
    18121 ttccgcattg tcacgctgcc ttacgtccat cagattgaag aggcgatggg caggctggca
    18181 agattcctgc aaacctaccg ccaataaggg gacggtttgt ctgccgagga taaaaaatgc
    18241 cgtctgaaac ggagattccc gtttcagacg gcattttcaa cagcaggaac gaatcaggca
    18301 aatttcagtc tgtcgccgtc ggcttctacc ctgatttcgc tttcgggcgc atagtttccg
    18361 gcaagcaggg cttttgccag cgggttttcg atttccgact ggatggcgcg tttgagcgga
    18421 cgtgcgccgt aaatcgggtc gaaaccggct ttggcgatga tgtccagtgc ggcatcggaa
    18481 acagtcaggc gcaggttttg tttttccaaa cgtttttcca aacctttgag ctggattttc
    18541 gcaatgctgc ggatgtttgc ctgatccagt ccgtggaaca cgactacttc gtcgatgcgg
    18601 ttgatcattt ctggacggaa atgttctttc acatcctcca tcacaacttc ttttaccgct
    18661 tcgtaatcct gaatgcccat ttgttggatg tgctggctgc cgatgttgga agtcatcaca
    18721 atcacggtat ttttaaaatc taccgtcctg ccttgcccgt cggtcaaacg accgtcatcc
    18781 aatacttgca gcaggatgtt gaacacgtcg ggatgggctt tttccacttc gtccaaaaga
    18841 atcacgctgt acggtttgcg gcggacttgt tcggtcaggt agccgccttc ttcgtagccg
    18901 acatagcccg gaggcgcgcc gatgaggcgg gcaacggcgt gtttttccat atattcggac
    18961 atatcgatac gaatcagatg atcttcactg tcaaacagga agcctgccaa tgccttacac
    19021 aactcggttt tacccacgcc ggtcgggcct aggaacagga agctgccgta gggtttattc
    19081 gggtcggcaa gaccggaacg gctgcggcgg atagcgtcgg acacggcacg cacggcttcg
    19141 tcttgaccga ctacgcggcg gtgcaacact tcttccattt tcagcaactt gtcgcgttcg
    19201 ccttccatca tttttgacac aggaatgccg gtcatacggg aaacaacttc tgcgatttcc
    19261 tctgcgccga cattattacg caagagtttg tttgccggtt ttgtgctgtc cgtatctgcc
    19321 cgttcggcgg ctgcacgctg tttttccaaa tgctccaaat cttcatacat caattttgaa
    19381 gccagtgcca aatcgccttg ccgttttgcc tgttcgattt taattttgac ttcgtcaatt
    19441 tgtttcttaa tattagcagc accgtctgaa atcgcttttt cggctttcca gatttcgtcc
    19501 aaatcggcgt attctttttc caaaccgtcg atttcctcat ctatcagttc caaacgtttt
    19561 ttgctggcat cgtctttttc tttttcaacg tgcgcctttt ccatccgaag ctgaatcaga
    19621 cggcggtcga ttttgtccat tgcttccggc ttggtttctt tttccatctt gacacggctg
    19681 gcggcttcgt caatcaaatc aatcgcttta tcaggcagga agcggtcggt aatgtagcgg
    19741 tcgctcaact ccgctgcggc aacgatagca gggtcggtaa tatcgatacc gtggtggatt
    19801 tcataacgct cctgtaaacc gcgcaagata gcgatggtgt cttctacact tggctcgcca
    19861 accaatactt tttggaagcg gcgttcgagt gccgcgtctt tttcgatgta ttggcggtat
    19921 tcgtccaagg tggtcgcgcc gatacagtgc aattcgccac gtgccaaagc cggtttcagc
    19981 atattgctcg catccatcgc accgtcggtt ttgcctgcgc cgaccaaagt atggatttcg
    20041 tcgatgaaaa tcagagtgtt gccgtcgtct ttcgccaagt cgttcaacac gcctttcaag
    20101 cgttcttcaa attcgccgcg gtatttcgcg ccggcaatca atgccgccaa gtccaaaacc
    20161 agtaggcgtt tgttgcgcag ggattcgggt acttcgccgt tgacgatgcg ttgtgccaaa
    20221 ccttcgacga tggcggtttt acccacgccc ggttcgccaa tcagcacagg gttgtttttg
    20281 gtacggcgtt gcaatacctg aatcgcacgg cggatttcgt cgtcacggcc gataacgggg
    20341 tcgagcttac cgtctcgggc gcgttgggtc agatcgagcg tatatttttt caaagcatcg
    20401 cgttggtctt cggcattggc atcgttcacg ttttgtcctc ctcgtaccgc gtcaatcgcg
    20461 gcattgatgt tttgttcggt cgcgccggct tctttcaaaa ttttgccggc cgcatcgttc
    20521 tgctgcacca aggcaagcag gaaaagttca ctggcgatat aggcatcgcc gcgtttggtt
    20581 gcggctttgt ccatcaggtt caacaccgcc tgcaattcgc ggctgggcag aatgtcgccg
    20641 ccctggccgg acactttcgg caggctgttc aaatgctgct gcaaacgctg tttcacctgc
    20701 ggcacgttca cgcccgcatg agccaagagc gcggcagcac cgctgttttg gtcgtcaagc
    20761 agggctttca gcacaaagcc cgcttccaga tagctgctgt ccgcagccaa cgccaaactc
    20821 tgagcttctg caagggcctg ttggaatttg gcggttaatt tgtcgtatcg catttttgtt
    20881 tccttttcaa aatgtccgct gtcgaagcct atatgtgcat aattgtggat aactcaagtt
    20941 ctgttttctg tttttctata tttaattcga tatatcattg aatttaaagt atataaaaat
    21001 gtataataat gtgtataact atatcttctt aatatggaaa agtctgttgt cggctggatg
    21061 taggtggcaa atcgggtata atcggcgcat ctttttccct ttcagacggc attgatgccg
    21121 caaggacatt tttatgagca aaaaacgagt tctgaccggc gtaaccacca ccggcatccc
    21181 gcatctgggc aactacgtcg gtgctatccg ccccgccgtc cgcgcggcgc aaaaccctga
    21241 taccgaatcc ttcctcttcc tcgccgatta ccacggtatc atcaaatgcc acgagccgga
    21301 gatgattcac caatccaccc aagccgttgc cgccacttgg cttgcctgcg gactcgaccc
    21361 cgagcgcacc accttctacc gccaaagcga cattcccgaa gtgatggaat tgaactggat
    21421 tctgacctgc atcaccgcca agggtttgat gaaccgcgcc cacgcctaca aagccgccgt
    21481 gcaggcaaat gcggaaaacg ggcaggaaga ccccgatttc ggcgtggaaa tgggtttgtt
    21541 cagctatccg attctgatga ccgccgacat ccttatgttc aacgccaacg aagtgcccgt
    21601 cgggcgcgac caaatccaac acgtcgaaat ggcgcgcgac atcgccggcc gcttcaatca
    21661 ccgctttaaa gaaatcttta ccctgcccga agtgaaaatc gacgaaaacg tcgaactctt
    21721 ggtcggtttg gacggacgca aaatgtccaa atcctacggc aacaccattc cgctttggga
    21781 aaacgacaaa aaaacccaaa aatcggtcaa caaaatcatc accaatatga aagagccggg
    21841 cgagccgaaa cagcccgacg aaagcccatt gtttgaaatc tacaaagcct tctccacgcc
    21901 gtctgaaacc gcagcgttca cgcaaatgct tgccgaaggc ttggcttggg gcgaggcaaa
    21961 aaaacttttg gcggcgaaaa tcaacgccga actcgccgaa ccgcgcgaac gctacaacga
    22021 gctgaccgcc gacccttcgc aaatcgaaga gattttgcag gcaggcgcgc aaaaagcgcg
    22081 taaagaagca cgcgaattgt tggacaaagt acgcgatgcg gtcggcatcc gcccgttgaa
    22141 ataaacccga tgccgtccga acctcttaaa ggcttgccgg aacaaaacgt cgtccggctg
    22201 accggcaagc atcccaacaa cttggaagcc gtcagcggca aatgcatgga aaccgacgat
    22261 aagggcggtc cggtaggctg ggcggcaaac ggcgtgtgcc ataccttgtt tgccaaactg
    22321 gtgggcaata tcgccgaaga cggcggcaaa ctgacggatt acctgatttc gcattccgcc
    22381 ctgcaaccct atcaggcagg caaaagcggc tatgccgccg tgcagaacgg acgctatgtg
    22441 ctggaaatcg acagcgaggg ggcgttttat ttccgccgcc gccattattg aggtattcgg
    22501 acatcccgga atatatttgg gtttttcaaa ccttgcgaaa aaaagtccgc accgcaggga
    22561 aactcaaaag gagggggata tgtgttacaa ttttccgaac tgttttcata aaatagtttt
    22621 cggacgtgtt tcaatatggc attgatgccg ccttattgtt cgagaaaaaa acctttatat
    22681 ttaaatataa tgggttttaa ctaaacggga aaccgttttc tctccggccg atgggcaaaa
    22741 tcagccgatt gatggaacac ggtccgattt ttaagcaaaa ccttaatcaa caactcaaga
    22801 aggataagac atgaaaaaat tattgattgc cgcaatgatg gcggctgcct tggcagcttg
    22861 ttcgcaagaa gccaaacagg aggttaagga agcggttcaa gccgttgagt ccgatgttaa
    22921 agacactgcg gcttctgccg ccgagtctgc cgcttctgcc gtcgaagaag cgaaagacca
    22981 agtcaaagat gctgcggctg atgcaaaggc aagtgccgag gaagctgtaa ctgaagccaa
    23041 agaagctgta actgaagcca aagaagctgt aactgaagcc aaagaagctg taactgaagc
    23101 agctaaagat actttgaaca aagctgccga cgcgactcag gaagcggcag acaaaatgaa
    23161 agatgccgcc aaataatttg ttgccttggc aaaaatgatg ggatgccgcc tgccggcaac
    23221 ccaaacgaaa ccgcctgaag attttcaggc ggtttttgag ttgtatgccc cttgtttttt
    23281 acgcctgcat gaccgttccg agcagatgtg ccgcgcatat cgggatgttg cggacaggca
    23341 aatgccgtct ggacagggtt tggacggcat ttgccccgca aggttcagac ggcctcgact
    23401 ttaaacggca tattgatttt ttgccattgg acgctttcat attccaatgc gtcggcaatc
    23461 aggggatggc gttccagcca ttccctgtca atacgcagga tgaagccgca gctttccgta
    23521 tccgtgcgca actgcatgtt tttcgggaaa gacaggtctt ggcgcgaacg gcagaacagt
    23581 gcggcaaggc gcagggacaa aacggcatac cacaacattt cgttggtgcc gatgatgccg
    23641 ctcatttttt tcatatcgcc gcgatgaccg atgaccagtt gggcaagtat ggtctgttct
    23701 ttgcgtgaga aacccggcat atcggcgttt tcgaggatgt aggcggaatg cttgtgatag
    23761 ccggtgtggg cgatgtccaa accgatttcg tgcagcgcgg cggcgcgtcc gagatactgt
    23821 tgccacaagg caagctcttg aactgtaacg tttttagcgt ggcagaggct gtccataaag
    23881 gtttgcgcgg tctcggcggt gcgtttcgcc tgattgaggc tgacgtggta gcggtgttgg
    23941 aactcggcaa ccgtttgtcc gcgcatatct tcgtttaaac cgcgcccgat caaatcgtaa
    24001 aacacgccgt cgcgcagggc ggcttcggtt acggtcatcc tgtcgagttt catttcctca
    24061 aacgccgcca tcatcacggc aagtccgccg gcaaaaactt cgatgcgttc cggtttcagg
    24121 ttttcaaatt tggctttttt gaccgaaccg gcttcgatga tgcgttcgga gagggcgcgc
    24181 atacctttgt aggtaatgtc cgcctcttgg ggcatttcgg cggcaagcac gtcgcggatg
    24241 gattttgccg aacccgatgt gccgacggcg aaatcccagc cttcgcgcct catatttttg
    24301 ctgatgcgct ggatttcgtt gcgggcggcg gaaatggcgg cttggaagtc tttggcggtg
    24361 attttgtttt ggaagaagcg caggctgtag gtaacgcagc ccaagggcag gctttcggta
    24421 atgtcgggat tcagcgtcga accgatgaca aattctgtcg aaccgccccc gatgtcgata
    24481 accagcattt tgccgccgcc gggggggagg gtgtggatca cgccggtata aatcagccgt
    24541 gcctcttcgc gcccggcgat gatttcgatg gggaaaccca gtgccgcttc ggccttggga
    24601 aggaaatctg cgatgttttt ggcgacgcgg aatgtattgg ttgccacggc gcgcacctgt
    24661 tcagggcgga agccgcgcag gcgttcgccg aattttgcca ggcagtccaa agcctgttct
    24721 tgggaagcgg cactcaggtt tttctgttcg tccagaccgg cggcgaagcg caccatctgt
    24781 ttgaacgaat cgataacttt taattgtccg ttgttgtttt cgcaaatctg gaggcggaaa
    24841 ctgttggaac ccaaatcgac ggaggcgagg acgtttgcgg gatgggtggt catggtggat
    24901 accggtgggg gaaaaacgca atgttactct gacggcgcag gcgttgacaa taaatgatgc
    24961 ggcggttttt gattctgccc acggatgttg ccgacggcat ttttgcgctt atttgaaatc
    25021 cttttccacg ctcatgaaaa tctgcgtgtt tttgcgtgtg taaaaacttt tcatattgct
    25081 gtcgattttc agatagcgga aattgagttg cggcgtaaag cccttccaag agattttgtc
    25141 gtgccacaac gacaggtttg cctgatattc gtggtctttg cgcgggaagc ggtacacaat
    25201 ggtcccgggt gcgtcaaaca tcctgcgggt atagcgcagg tttgccagca gacccaagcc
    25261 gccgtcgaac gttttgaccg cgccgacacg caaacccttg cggatggaag cctgttccgc
    25321 ctctttcgtt atgttgtgcg accagtccgc accgccgtaa agcagccagt ctttcggcgc
    25381 ggaatacatc agcgtcgcgc ccgccagcgg catatggctg tcgtatcggg cggcggtgcg
    25441 gtcttcctga taatgcttcc acatattgcc cgcgtttagt gtcaaccgcc agcgttcgct
    25501 caagcgttgg gagaaatcgg cattgaagcc gccgacgaaa ttgtatcggc tgccgcctaa
    25561 gaggttttgc tcgacaaacg gcacgatgcc gaacgagcgc gttaccgaac ggtttttata
    25621 gccgaacgac aggcgcaggc tctgttcgct gaaatctttg ttatcccaat aatgcacgcc
    25681 gccgccgctg atgccgccgt agaggaaatg atgcctgccc gcattgattt cgcgcgacac
    25741 gcctaagccc tagcgcaaac cgtgtgcctt ttgcggcagg ctgtcggcgg ttttcgtcca
    25801 gcttctgccc gcaaattcaa tcgttttttt ggacgaagcg ttgtttatag cggattaaca
    25861 aaaatcagga caaggcgacg aagctgcaga cagtacagat agtacggaac cgattcactt
    25921 ggtgcttcag caccttagag aatcgttctc ttcgagctaa ggcgagacaa cgccgtaccg
    25981 gtttttgttc atccactata acagcaaccc tgtcgccgtc attcccgcaa aagcgggaat
    26041 ccagtccgtt cagtttcggt catttccgat aaatgcctgc tgcttttcat ttctagattc
    26101 ccactttcgt gggaatgacg
//
LOCUS       DAWHXS010000015        24874 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWHXS010000015 DAWHXS010000000
VERSION     DAWHXS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.015"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(197..1366)
                     /locus_tag="WIE22_000497"
     CDS             complement(197..1366)
                     /locus_tag="WIE22_000497"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ4126639.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1549..1659)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1712..2608
                     /locus_tag="WIE22_000498"
     CDS             1712..2608
                     /locus_tag="WIE22_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ4126640.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2652..3716)
                     /gene="tsaD"
                     /locus_tag="WIE22_000499"
     CDS             complement(2652..3716)
                     /gene="tsaD"
                     /locus_tag="WIE22_000499"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ4126641.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPIHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            complement(3833..5020)
                     /gene="ccsB"
                     /locus_tag="WIE22_000500"
     CDS             complement(3833..5020)
                     /gene="ccsB"
                     /locus_tag="WIE22_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ4126642.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRTEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(5013..6965)
                     /locus_tag="WIE22_000501"
     CDS             complement(5013..6965)
                     /locus_tag="WIE22_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ4126643.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7242..7865)
                     /locus_tag="WIE22_000502"
     CDS             complement(7242..7865)
                     /locus_tag="WIE22_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ4126644.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8071..8700
                     /locus_tag="WIE22_000503"
     CDS             8071..8700
                     /locus_tag="WIE22_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ4126645.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8821..11217)
                     /locus_tag="WIE22_000504"
     CDS             complement(8821..11217)
                     /locus_tag="WIE22_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ4126646.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11369..12484
                     /locus_tag="WIE22_000505"
     CDS             11369..12484
                     /locus_tag="WIE22_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ4126647.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKLLYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12487..13086
                     /locus_tag="WIE22_000506"
     CDS             12487..13086
                     /locus_tag="WIE22_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ4126648.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13087..13734
                     /locus_tag="WIE22_000507"
     CDS             13087..13734
                     /locus_tag="WIE22_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ4126649.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13752..14297
                     /locus_tag="WIE22_000508"
     CDS             13752..14297
                     /locus_tag="WIE22_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ4126650.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14316..16601
                     /locus_tag="WIE22_000509"
     CDS             14316..16601
                     /locus_tag="WIE22_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ4126651.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQATAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17662..18174
                     /locus_tag="WIE22_000510"
     CDS             17662..18174
                     /locus_tag="WIE22_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ4126652.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            18254..19333
                     /gene="aroB"
                     /locus_tag="WIE22_000511"
     CDS             18254..19333
                     /gene="aroB"
                     /locus_tag="WIE22_000511"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ4126653.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
     gene            20300..21142
                     /locus_tag="WIE22_000512"
     CDS             20300..21142
                     /locus_tag="WIE22_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ4126654.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     NDRVAVIATLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            21171..21635
                     /gene="nrdR"
                     /locus_tag="WIE22_000513"
     CDS             21171..21635
                     /gene="nrdR"
                     /locus_tag="WIE22_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ4126655.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSTELADMVLKELLEHNTEAAVRFAALHKRFDNPSDFASWLAQAVKTDRKA"
     gene            21668..22777
                     /gene="ribD"
                     /locus_tag="WIE22_000514"
     CDS             21668..22777
                     /gene="ribD"
                     /locus_tag="WIE22_000514"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ4126656.1"
                     /translation="MFSDTDISMMEIALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPQLNVRDFPTLRQP
                     ARIVLDSRLRLPPYSHLVTDGQSPTLIATLERDEDKLRPYREHAHIRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNDNAF"
     gene            22972..24390
                     /locus_tag="WIE22_000515"
     CDS             22972..24390
                     /locus_tag="WIE22_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ4126657.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSSQLVSKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEENAPPARLSATAEFAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWL
                     FFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            24387..>24874
                     /locus_tag="WIE22_000516"
     CDS             24387..>24874
                     /locus_tag="WIE22_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4126658.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NAL"
BASE COUNT         6345 a         7352 c         5797 g         5380 t
ORIGIN      
        1 aatttaaacc agtacagcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
       61 tgaagcacca agtaaatcgg ttccgtacta tctgtactgt ctgcggcttc gtcgccttgt
      121 cctgattttt gttaatccac tatataaaaa tgccgtctga actgttcaga cggcattttt
      181 gattttcaaa ccggaattac agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc
      241 gctcccaagt gaactcaggt tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt
      301 aaatcgggcg caagagatcg agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt
      361 cgcgaactaa ggtaatcagt ttttcctcgc tgattttgcc tgtgccgaaa gtgtcgatgg
      421 aaatcgaagt cggttcggca acgccgatgg cgtaggaaac ttggatttgg cattgggttg
      481 ccaaaccggc ggcgacgatg tttttcgcaa cgtagcggca ggcgtaagcg gcggaacgat
      541 ccactttgga cgggtctttg ccggagaacg caccgccgcc gtgcggagcc gcgccgccgt
      601 aggtatcaac gatgatttta cggccggtca aaccgcagtc gccttgcggg ccgccgatga
      661 cgaagcggcc ggtcgggttg atcaggtatt tggtttcgtc ggtcagcagt tcagacggca
      721 ggacgggctt gataatctgc tcaatgacgg cgtttttcaa ctcttcataa ccgatttccg
      781 ggtcgtgttg cgtagagagg acgacggtat caatgcgttt tactttgccg gattcgctat
      841 cgtaaaccac ggtcagttgg gcttttgcgt cgggacgcag ccaaggcagg aggccgtctt
      901 tgcgtaattc gctttgacgc tgcatcaggc ggtggctgta atagatggca aacggcatca
      961 gggtcggggt ttcgtcgcag gcatagccga acatcaaacc ttggtcgcca gcgccttggt
     1021 tcaagtcaat gccttcgcct tcgttcacgc cttgggcgat gtccggagat tgctggtcgt
     1081 agtacacgcc gaccgcgcag ccgttggcat caaagcccag ctcggaggag ttgtagccga
     1141 tgcgtttgat ggtttcgcgt gcgactttga tgtagtccac ttgggcggtg gtggtgattt
     1201 cgcctgccaa tacgcacaag cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg
     1261 ggtcttgcgc caagatggca tccaaaatcg cgtcggatac ttggtcggca actttgtccg
     1321 gatggccttc ggataccgat tcggaagtaa acagatattc gctcattgag attttccttg
     1381 aaaaacaaac catcttcttc agacggcatg ttgtatgaac ataatgtcga cagcgggaaa
     1441 tatagcaaaa tttccctatt cataccattc agttgagaaa tattcccatt tgaatggcac
     1501 tttggaatct ctgcccgtac gtttcttaca ggcaaaaaaa ttcccgcatc aagcgggttt
     1561 ggattgctct ggtgagccac atcggctttt caaccgtcca ccttactttt ccttttgaaa
     1621 agcaggttgg catggaattc ccaactctta atgcagcgcg gatggtagca gaaaagcctt
     1681 tccgccgcaa tacttccctt tttcagacgg catgtttcgt ttacaattcg ggctgtttcc
     1741 ccctttgcga accgccatgc acatcctgtt gaccgccctg ctcaaatgcc tctccctgct
     1801 gccactttcc tgtctgcaca cgctgggaaa ccggctcgga catctggcgt tttacctttt
     1861 aaaggaagac cgcgcgcgca tcgtcgccaa tatgcgtcag gcaggcatga atcccgaccc
     1921 caaaacagtc aaagccgttt ttgcggaaac ggcaaaaggc ggtttggaac ttgcccccgc
     1981 gtttttcaga aaaccggaag acatagaaac aatgttcaaa gcggtacacg gctgggaaca
     2041 tgtgcagcag gctttggaca aacacgaagg gctgctattc atcacgccgc acatcggcag
     2101 ctacgatttg ggcggacgct acatcagcca gcagcttccg ttcccgctga ccgccatgta
     2161 caaaccgccg aaaatcaaag cgatagacaa aatcatgcag gcgggcaggg ttcgcggcaa
     2221 aggaaaaacc gcgcctacca gcatacaagg ggtcaaacaa atcatcaaag ccctgcgttc
     2281 gggcgaagca accatcgtcc tgcccgacca cgtcccctcc cctcaagaag gcggggaagg
     2341 cgtatgggtg gatttcttcg gcaaacctgc ctataccatg acgctggcgg caaaattggc
     2401 acacgtcaaa ggcgtgaaaa ccctgttttt ctgctgcgaa cgcctgcctg gcggacaagg
     2461 tttcgatttg cacatccgcc ccgtccaagg ggaattgaac ggcgacaaag cccatgatgc
     2521 cgccgtgttc aaccgcaatg ccgaatattg gatacgccgt tttccgacgc agtatctgtt
     2581 tatgtacaac cgctacaaaa tgccgtaacg aaaataaaaa tgccgtctga acaatttcag
     2641 acggcatttt gtcatttgac gatttccgac agcggccagc gcggacggac attgaacgca
     2701 ccgacctccc tgcccttgcc caggtgcatc gcaccggcaa aggcaatcat ggcaccgttg
     2761 tccgtgcagt atgccgtcgg cgggaaaaac acgctgactt tttcggacgg atgtttcggc
     2821 ttgcctgttg gggtcgggat ttgcaccgtc atgctgccga aagtttcacg gagtttgcgg
     2881 tttgcaccga ccccgccggc gaccactacg gttctgaacc ctgtctgcaa cagggctttt
     2941 ttcactttcg ccgccaacac atcgactacc gcatcttgaa acgcacggca gatgtcgttg
     3001 cgtgtctgct caggaatgtc atccgccccg ttttccgcgc gcactttctc gacggcagtc
     3061 aaaacagcgg ttttcaaacc tgaaaaactc atctgcaaat cgtcggaatg aatcatcggg
     3121 cgcggaaaaa caaacgcttc gggcctgccc gattccgcaa gttccgacag tttcgcaccg
     3181 cccggatacg gcaagcccag cagtttcgcc gttttgtcga atgcctcgcc cgccgcatca
     3241 tcgacgctct cgcccaaaag cgcgtagtcg cctatgcccc tgaccgccat aatctgcgta
     3301 tgcccgcccg aaaccagcag cgcgacaaaa ggaaagtcgg gtttttcctc cgccaacagc
     3361 ggcgacagca gatgtccttc caaatgatgg atggggatga cgggcttgcc caatgccaaa
     3421 gccagtgcat tggcgtagct cgaacccgcc agcagcgcac cgcccagacc gggtccctgc
     3481 gtaaaggcaa ccgcgtcaat gtcgccatac gatgcacccg cctgcgccaa acagccttcc
     3541 gtcaacggaa caaggcggcg gatatggtcg cggcttgcca attccggcac aaccccaccg
     3601 tattcggcgt gcattgccat ttgagtgtgc aggcagtgcg cccgcaatcc tcgttccgta
     3661 tcgtaaagcg caacgccggt ttcgtcgcaa gaagactcga ttcctaatac caacatggtc
     3721 tgatgccgtt aaaaactgaa aaacgtattt tagcggattt cggcacgact gccgtatccc
     3781 aaaaacggaa catgccgtct gaagaccgtt cagacggcat cgtcgcaccg tatcaaagcg
     3841 ttccgtaaga atgcagcccg ctcaaaaaca tattcacgcc gataaaggca aatgcggtta
     3901 cgaacaaacc gataatcgcc caccacgcca gcactttgcc gcgccaaccg gcaaccagcc
     3961 gcaagtgcag ccaaacggcg taattgagcc agacgatgaa cgcccacgtc tctttcgggt
     4021 cccaactcca atagcgtccc caagcatccg ccgcccacag cgcacccaaa atggtggcaa
     4081 tggtaaagaa cagaaagccg acggcaatcg ccttatacat aacttcctca atcaacacgg
     4141 attgcggcag ccacgatttc ccgccttttc cttccgtacg cagggaaacc agttcggcaa
     4201 taccgagcat cgcggaaatg caaaacgcgc cgtaaccgat aaagtttgcc ggaacgtgga
     4261 ttttcatcca ccaagattgc aatgccggaa tcagcggctg gatggtatgc gcctcgcggg
     4321 acacgctgta ccacaagaca aatccaacca cgaccgccat aaagccgaac acgaagccgc
     4381 ccaatttctg tatggcgaac ttaccttcat aataaagata catcagcgcg gtaatcacca
     4441 aaaacaggat gaacacttca tacaggttgg aaaccggaat atgccccgca tcgggacgga
     4501 gcagatagct ttcgtgccaa cgtaccagca gaccggtaaa gcctgctacg gcagacaccc
     4561 atgcaaacac ggttcccata cccaacagcg tgttggtcgg cacatttttt acgcttgcca
     4621 aaaccgcgcc cgaaatatag gcgaacaggg cgaagaagac aaaggcgcac tgccacataa
     4681 tcgccgactg gctgctgagg aaataccgca acaggaaaat ctctgccgat ttgatgttgc
     4741 cgccatacaa accgacggcg gcataggcaa gcaatacgct taaaggaaca aaccagcgca
     4801 tcggcttgaa aaaccagccc aaaaatacgg caataccggc actcgcccac aacatgaccg
     4861 tttcgtaaat gtccatatgc ataccggaac gggtctgtac gaaaaccgta gccgcaaaaa
     4921 ccagcacggc aaatacccaa tcccaaagat tcagattgct gatcaaagac ttctgaatca
     4981 gcagctcgtg ttccggaagg gttttatagt gttcagtcat gattcaagtc cttgccgagc
     5041 cgttgcagac tctcgacgtg ttttggaaat tccttctgca aatcccgttc gctgcgggcc
     5101 gaagacatgg caaaacggat tttgccgtct gaaaacaata cccacgcccg tttttcgcgc
     5161 acataaaaca tcaataccgt acccaatacc aacagcaccg agccgagata gaccaaaagc
     5221 gcacccgggg aacgggtcat ctgcaaaccc gacgaacgca cctcggaaaa cccatcaagt
     5281 tgcagcagca taggcgcggg atattcggtc aaacccgtgt acgcatccat actgtgcagc
     5341 aggaaacggt tccgcgcttc atcctgctgc cattcgggca agccgtaccg gcgtatggtt
     5401 tcatccaaag cagcgttcat cacgccgtaa agcatttcgt agaaataacc ctgcatctta
     5461 tcctgctgct ctttcggaat attggacgta ataaattcgt ccaatcccaa atagcctttt
     5521 tgtgcaaaga tgttcagcgt gttttccgca gccagcatga attgttcgcg gatttcggca
     5581 ggtgcgtctt tggttgcgtc ggcaaccaga cgtttgcgcc cttccccatc tttcaaaaac
     5641 tcgcgcaatg ccataaaggt gtccgctttc aactgcttgt ccaaggggat acgcagccag
     5701 cggtattgct gctgcaagcc gctgcgcgtg ccggtaatcc aaaaataatc ctgttcctgc
     5761 aaaaccggca gcatatagtt tttatattcg accgcctgcc ctgccgcatc acggatacgg
     5821 taaacaatgg aagggccgat attggtgtat tttttacctt cctgagtaac ggcgcggaca
     5881 tcgttcagcg tggatttcag gcttttttcc cgttccgcgc cctcgctcat gtcctccaca
     5941 ttcatagaag tgaactgatc gaactcaaga cgatatttgt gtttgccaat ttccaacgga
     6001 aactggtgta tggatgttgc cttcaacacg acaggctcgc gcgaagcatc acccaaattc
     6061 cacgccttga atgtcaaatc cgaaccgccg tcggcaaaac tcgcctgata aatcgtgatg
     6121 ccgtgcaagg tcaaaggatg gttcacgcgg atggtgcgct cgagtttctc accggttgcc
     6181 ttatccgtta cttcaatatc actggcaaaa tcgcgcggca tacccgtatt gtaaaaatcg
     6241 atatggaatt ttttcagttt gacttcaaaa ggcaagtcct gaaccaatat cccgttgtcg
     6301 gcattcagga aaaccacatc cgcactctgc ccctcggaaa tattgacgtt gcccctaaat
     6361 gagagattgg acgcacccaa aatactttcg ggcttgaaat ccttggcata aaccgcctga
     6421 ttgtccggaa caatccgacc ggtcagcata cccagtttca acagcaggtt actgtctatc
     6481 aacccgccca ggcaaatgac aatcaaagca acatgggcaa agatatagcc ccatttgttc
     6541 attgtgcctt ttttggcggc aatcagaacc gacccgtctt cacggttaat ggtttttccc
     6601 tgaaaacctt gtacttccag ataacgtttg gcaacctcgg gcgcaatttt tacatccaac
     6661 agcgaagaat ggcgcatcgc cgccagagat ttttctttaa ccttttcccg aaaagacttc
     6721 atttcgcgcc agaacggcgg cacattgcga atcaggcaca aactggtaga aaccaccaaa
     6781 aacatcatga taacgacaaa ccatgccgaa gcatagacgt catacagtcc cagaaaacca
     6841 aaaatctgcg cccaaaacga tccgaatttg accaaataat ccgtctgcgg ctggttttgc
     6901 tgcaacaccg taccgataac cgatgcaata cccagcagac tgagcaaagc gactgcaaag
     6961 cgcatggagc tgaaaaaagc gaaccacgga cgggaaagaa gtgggggaga tctacgggat
     7021 ttactcattg tgtgtttatt ccgccatcag gaatatggga aagcagaatt gggcaaacag
     7081 aaaacaacgt cccgattcta ctgtcttgat gctttttatt tcaagacaat gaagacagcc
     7141 tgcatcgatt ccaacggttg cgattgaaaa aacttatcgc agaattgcct gaagccgtct
     7201 gaaaactttt cagacggcct ctaaaacaga ctattgcgga attaacgcaa accttggata
     7261 aagttggcga ccgctttcaa atcttcttca gacatacggt ttgcaatatc ttccatgatg
     7321 gtatttttac gctgaccgga cttgtaggca ttcatctgtt caacaatata tgcctgatgc
     7381 tgaccgccca aacgcggata agcctgaatt tcgcttccgc ctcccggcat acccgcaccg
     7441 ctcggaccgt ggcaggacat acacgccggc acttttttat cgctcaaacc gccgcgatag
     7501 attttcgcac ccaattcggg attttcctta ggattggctt caccggattt gggctgctgt
     7561 ttggcataga atgcggatac gttcaaaata tcctgatcgc tcaaattcat taccaccggt
     7621 ttcatcacag ctgccgaacc gtgggtgcgt ttaccgtcgc ggatgccgat agtttgatga
     7681 tagatgtaag cagtatgctg tgccgccaaa cgcggataca tcgcaatgcc gctgttaccg
     7741 tctgctgcat ggcaagccgc acaaaccgtt gcggcaacct gtttgccttt ttccacgtct
     7801 gctttgggag aggcggaaac cgcaccggca gccaaaacaa aggccaataa agtcaatcgt
     7861 ttcatggagt gctcctgatt acagcattgg ataacgcaac aatgctcttt ttatattcaa
     7921 atacgggatt tttgacccga ttaaaaccga tgattctgta aacgtgttat tctatactaa
     7981 atttacatta aattaccact gtgtttcaca taaaaccaac cgcatatttt tgctgtcgga
     8041 caaacggcgg cggaaaacaa ggatatgccc atgaaccttt ttcaaaacgc caaattcttc
     8101 acgacggtca accaccttaa agacctgccc gacacccctc tcgaaattgc ctttgtcggc
     8161 aggagcaatg ccggaaaatc cagtgccatc aataccctga ccaaccatgt ccgtcttgcc
     8221 tacgtttcaa aaacacccgg acggacgcag catatcaact tcttcgagct gcagaacggc
     8281 aattttatgg tcgatttgcc cggctacggt tatgcccaag tccctgaagc agtacgcgca
     8341 cattgggtca atctgctcgg cgactatctg caacagcgca aacagcttat cgggctggtt
     8401 ttgattatgg atgcccgcca tcctttaaaa gaactcgaca tccgtatgct ggattttttc
     8461 cacacgaccg gcagaccggt tcacatcctg ctgtcaaaag ccgacaaatt atccaaaaac
     8521 gaacagataa aaaccctgtc ccaagtcaaa aaactgctca aaccttattc cgacaggcaa
     8581 aacatcagcg tacagctgtt ttccagcctg aaaaaacaag gtattgacga ggccaaccga
     8641 actgtcggaa gctggtttga cgcagcagat gccgccgctt cctctccaga ggaaaactga
     8701 ccccaattat acggaaaccg tatttccccc acttgaccga ccgcaaacat ttaaaaaatt
     8761 gccactgcca aatctaaaat gccgtctgaa agacttttca gacggcattt tgcggagtct
     8821 ttaaaacaga gaatccaact gctgctgttt ggaaccagta ttactcggaa gcaccggcgt
     8881 ttcctgcata tcttggcgga cttcgtcatc cgccgcctgc cgtccgcctt ctgccgcgcc
     8941 cccgtcatct tcttttgccc gtcgggaagg ttgcggcgca ataccgctgt tgtccagcgt
     9001 caagcccgga tcggttacca tacgttcctt catatagtat tcgccattgc tgctgaccac
     9061 accttcaggc attttcatcc ccttgccctg ctttcctttc aacgcaaaac gcatatagtc
     9121 cacccaaacc ggcaccgcaa tcgtaccgcc gtagccgaca cgccccatac tcttaggttt
     9181 gtcgaagccg atatatacgg cagtaaccac atcagggtta aaaccgacaa accacgcatc
     9241 cttattgtca ttggtcgtac ccgttttacc ggcaatatcc gttcttccca acgcagctgc
     9301 cccccttgcc gtaccgacac ggaccacatc ctgcataatc ttatacataa tataggcatt
     9361 gcgcggatcg attgcctgag gcgcattttg cccggccacc aaaggttgca tttgggcgcg
     9421 caacctgccg tctctgtcat aaatcttatc gattacgtgc gaagaaaccc tatatccgcc
     9481 gttcgcaaat acgctatatg cctccgccac tttcaacggc gttgtctcgc ccgtacctaa
     9541 agccatagac aggcttgccg gcagctcgga cgacctgaag ccgaaacgcc ggatatactg
     9601 ttgcgcgtaa ccgacaccga tagacatcaa aatacggatg gaaaccatat tcttggaagc
     9661 cgtcagagcc tgtctcaaag taatgtagcc ggaatatctg ccgtctgaat ttttaggtgt
     9721 ccaaaccgaa ccgttcggcc ctttccccgg cagggaaatc ggcgcatcgt taaccaccgt
     9781 ggacgcggtc atccccttag ataatgccgc cgaatagaca aacggcttaa aggtcgaacc
     9841 cggctgccgc attgcctgaa cggcacgatt gaatgttttg ctgtgaaaat cataaccgcc
     9901 gaccagcgcg cgcacagctc cggtttttgc atccagcgaa accaaagccc cctgcagcaa
     9961 cggctcttga accaccgccc aacgcccgcc gttgtttttg acacggatga ccgcgcccct
    10021 gcggatacgg tcctccccca ttttttcatt attgaccgcg cgggccgcaa agcccaaggc
    10081 gcgcctgtca agcgtaaccc gcctgccgcc gggcagctgt atgacgacat ttttcttttt
    10141 agtcacatcc aacacaacgg cgggaaccat tttatcgacg gtatagagtc ccgacagata
    10201 ctggctgaca gtctcctcga catcttcact cttactcaaa tcgatatagt tttccgcacc
    10261 gcggtagctg ctgccgcgat cgaaattccg tagagccttg cgcaatgcct cggttgccac
    10321 cttctgatga tcggcgcgga ccgtggtata aaccttaaaa ccctgcgtat aggcatcttc
    10381 accgtatttc tcatacagtt cctgacgcac catttccgcc acatataaag cactctgatc
    10441 gattttccga acaaaccgct cgtaatgcag ttcctcattc aacgcctgat cgcgctgttg
    10501 cacggtaatc atcttctcct cgagcatatt gttcaaaata tacttctggc gcaacttggc
    10561 acgttctgga ttaacaatcg gattataggc agacggagcc ttgggcagtc ccgcaagcat
    10621 ggcggcttcc gccaaagtca aatctcggac attcttattg aaatagattt gcgcggcaga
    10681 tgcaaaacca taggcgcgct gaccgaggta aatctgattg aaatacaact cgaggatttt
    10741 gtctttgctt aaagactgct cgattttata ggcaagcaac acctcattga atttgcgtgt
    10801 gaacgttttt tcactgctca aataaaaatt tttcgccacc tgctgcgtaa tcgtactcgc
    10861 acccgactgc acgctgccgg acacgacatt gccgacggca gcgcgggcaa caccccaaac
    10921 atccaccccc caatgccggt aaaagcgttt atcctcggcg gcgataaccg cattccgcaa
    10981 cacctctggg aaatcgccga tttttgtaaa ttcgcgccgc tgctccccat acataccgat
    11041 gacttcccca tccgccgaat aaatagtcaa cggcatttta ggctggtaat gctgcaaaga
    11101 atccaaagac ggcagtttcg gatacgttac caaaatagca atggcaacca aacccactcc
    11161 aaatacacaa aacccaaaaa ccaaaccaaa acaagtcgtt aaaatctttt taatcatagc
    11221 tgaataataa tttaccatta ttggtattaa ataaagtaaa atagcaaccg atttctacaa
    11281 agcacggttt caatgtgcaa agaacaagga atccattacg gataccgaaa cggttactca
    11341 ctgtacaaat aaagcaggaa ctttcatcat gcgcttgttt aaaagcttga aaaaccctaa
    11401 aaaaacagat gccaagctcc ctaaaaaatc ttcgggactc aataaccgcg cggcaatcgg
    11461 catcgatatc gaccagcatt ccatcaaaat ggtccaattg tcaggacgta gtttaaacca
    11521 aattcaattg gaaaaatacg tcattgccaa attaccaaag aatatcattc aaggcaataa
    11581 agtccaaaat tacgatcaac ttgttacata tttgcaacaa gcctatgcca aactgggtac
    11641 ttcgtgcaaa aacatcatcg cgtccgtccc gcaaaatctg gcaaccatcg aacaattgac
    11701 ctacacagac aaagatgcag aattagacct gcaggggttc gtggagtcct ccatctccga
    11761 agtcagctcg atatcgctcg aagaagccaa ttacgactat caggtcttgt cccaatcggc
    11821 cgccggcgaa gctgtgttgg ccgtcgcatc gagaaaggat gaaatcgaac ccctgattga
    11881 cgcattcaac gccgccggta tgaaattatc cgcgcttgat gtggacattt tcggacaata
    11941 caacgcctac gcgctatgga taaaccattt cgcccccgag cttgcggccg aaaaagtcgc
    12001 cattttcggc gtatatgccg cacagaccta cgccttggtc atccaagacg gaaaactcct
    12061 atacaaacag gaaacctccg tcagcgaaga acagctcaac caactcatcc agcgcaccta
    12121 tcaggtaaca gaagaaaaag cggaagaaat catcaactcc ccgcaaaaac cttccgatta
    12181 ccaagaaagc gtggcaaact atttcaacca gcagattacc caagaaatac aaagggtctt
    12241 gcagttttat tacaccacgc agaccgcaga cgatatgacc gacatcaagc atatcctgct
    12301 gaccggggaa gcggcgcgcc aggaaggcat cgcccaaacc gtcgcctcac aaaccaatgc
    12361 agatgtacaa tgcgtccatc ccgcgcgtta ttttgcggac aacctcaaaa cagacaaaca
    12421 acaattcgaa cttgatgcgc cgacactgac cagggcgttc ggtttggcgg tacggggatt
    12481 ataattatga acaatttaat caaaatcaac ctcctcccct acagggaaga gatgaacaag
    12541 cgcaaacagc agcagtttaa aacgctgatg tacggtgccg tgctgacggg cgttgccgcc
    12601 gttgcggcaa cctacctgtt tatcgacaat atgatcaata accagtcgga aagaaacacg
    12661 ctgctggaaa cctccatcgc acacttggat accgagctgt cggaaataca aaagctcaaa
    12721 caggaaaaag atgccttcct gattaagaaa aacaaaatcg aggagctcca gctcaaacgc
    12781 ctccaagccg caaaaatcct cgacagcctg aatgaggccg tccccggaag cacctacctg
    12841 acctcgctgg atgccgttac cgccgactct tatcggctca gcggcaggac atccagcgac
    12901 aaccgcgttg ccgccatgat gagggcgatg cccaataccg gcatattcaa gcaacccgaa
    12961 ttgttaagca tcaagaaaaa caattcgcat caagaattta cccttcaggc aacattacaa
    13021 cccatcgtaa aggcggccga atccaaagag aatccggctt cgggaaacgc acaggaggca
    13081 aactgaatgg cttctaaatc atctaaaacc aacttggatc tcaacaacct tcacctgctc
    13141 aaccttcctg ccaggctttt tatcgccctg ctggccgttg ccgccgtgct ggggctcggt
    13201 tatgccggat tgttcaaaag ccagatggaa tcccttgagg aatacgaagc aaaagaaacc
    13261 gaactgaaaa acacctacaa acagaaaagt atcgacgcgg ccagcctgaa caacctgagg
    13321 gacgaacttg cctcaatccg ctctgccttc gatatcatgt tgaaacagct gccgacagat
    13381 gcagaaattc ccaacctggt tcaagagctt catcaggcag gttcgagcaa cggcctgcgc
    13441 ttggacagcg ttatgcccca acctcccgta gatgacggcc ccatcaaaag attaccctat
    13501 tccatttcca ttaccggaaa ttacgaacag atcagccaat ttacccgcga tgtcggcagc
    13561 ctctcccgaa tcattaccct tgagtcgctg aaaatcgccc aatctccgga aaacggcggc
    13621 aatcctgacg gcaagagcag catcctgaac ctcagtgcca ttgccaccac ctaccaagca
    13681 aaatccgtag aagagcttgc cgcagaagcg gcacaaaatg ccgagcaaaa ataacttacg
    13741 ttagggaaac catgaaacac tatgccttac tcatcagctt tctggctctc tccgcgtgtt
    13801 cccaaggttc tgaggaccta aacgaatgga tggcacaaac gcgacgcgaa gccaaagcag
    13861 aaatcatacc tttccaagca cctaccctgc cggttgcgcc ggtatacagc ccgccgcagc
    13921 ttacagggcc gaacgcattc gacttccgcc gcatggaaac cgacaaaaaa ggggaaaatg
    13981 cccccgacac caagcgtatt aaagaaacgc tggaaaaatt cagtttggaa aatatgcgtt
    14041 atgtcggcat tttgaagtcc ggacagaaag tctccggctt catcgaggct gaaggttatg
    14101 tctacactgt cggtgtcggc aactatttgg gacaaaacta cggtagaatc gaaagcatta
    14161 ccgacgacag catcgtcctg aacgagctaa tagaagacag cacgggcaac tgggtttccc
    14221 gtaaagcaga actgctgttg aattcttccg acaaaaacac cgaacaagcg gcagcacctg
    14281 ccgcagaaca aaattaagaa gaggattact ccattatgaa taccaaactg acaaaaatca
    14341 tttccggtct ctttgtcgca accgccgcct ttcagacagc atcggcagga aacattacag
    14401 acatcaaagt ttcctccctg cccaacaaac agaaaatcgt caaagtcagc tttgacaaag
    14461 agattgtcaa cccgaccggc ttcgtaacct cctcaccggc ccgcatcgcc ttggactttg
    14521 aacaaaccgg catttccatg gatcaacagg tactcgaata tgccgatccc ctgttgagca
    14581 aaatcagtgc cgcacaaaac agcagccgtg cgcgtctggt tctgaatctg aacaaaccgg
    14641 gccaatacaa taccgaagta cgcgggaaca aagtttggat attcattaac gaatcggacg
    14701 ataccgtgtc cgcccccgca cgccccgccg taaaagccgc gcctgccgca ccggcaaaac
    14761 aacaggctac cgcaccgtct accaagtccg cagtatccgt atccaaaccc tttaccccgg
    14821 caaaacaaca ggctgccgca ccgtttaccg agtccgtagt atccgtatcc gcaccgttca
    14881 gcccggcaaa acaacaggcg gcggcatcag caaaacaaca gacggcagca ccagcaaaac
    14941 aacaggcggc agcaccagca aaacaaacca atatcgattt ccgcaaagac ggcaaaaatg
    15001 ccggcattat cgaattggct gcattgggct ttgccgggca gcccgacatc agccaacagc
    15061 acgaccacat catcgttacg ctgaaaaacc ataccctgcc gaccacgctc caacgcagtt
    15121 tggatgtggc agactttaaa acaccggttc aaaaggttac gctgaaacgc ctcaataacg
    15181 acacccagct gattatcaca acagccggca actgggaact cgtcaacaaa tccgccgcgc
    15241 ccggatactt taccttccaa gtcctgccga aaaaacaaaa cctcgagtca ggcggcgtga
    15301 acaatgcgcc caaaaccttc acaggccgga aaatctccct tgacttccaa gatgtcgaaa
    15361 tccgcaccat cctgcagatt ttggcaaaag aatccgggat gaacattgtt gccagcgact
    15421 ccgtcaacgg caaaatgacc ctctccctca aagacgtacc ttgggatcag gctttggatt
    15481 tggttatgca ggcacgcaac ctcgatatgc gccagcaagg gaacatcgtc aacatcgcgc
    15541 cccgcgacga gctgcttgcc aaagacaaag ccttcttaca ggcggaaaaa gacattgccg
    15601 atctgggcgc gctgtattca caaaacttcc aattgaaata caaaaatgtg gaagaattcc
    15661 gcagcatcct gcgtttggac aatgccgaca cgaccggaaa ccgcaatacg cttgtcagcg
    15721 gcaggggcag cgtgctgatc gatcccgcca ccaataccct gattgttacc gatacccgca
    15781 gcgtcatcga aaaattccgc aaactgattg acgaattgga cgtacccgcg caacaagtga
    15841 tgattgaggc gcgtatcgtc gaagcggcag acggcttctc gcgcgatttg ggcgttaaat
    15901 tcggcgcgac aggcaagaaa aagctgaaaa atgatacaag cgcattcggc tggggggtaa
    15961 actccggctt cggcggcgac gataaatggg gggccgaaac caaaatcaac ctgccgatta
    16021 ccgctgccgc aaacagcatt tcgctggtgc gcgcgatttc ctccggtgcc ttgaatttgg
    16081 aattgtccgc atccgaatcg ctttcaaaaa ccaaaacgct tgccaatccg cgcgtgctga
    16141 cccaaaaccg caaagaggcc aaaatcgaat ctggttacga aattcctttc accgtaacct
    16201 caatcgcgaa cggcggcagc agcacgaaca cggaactcaa aaaagccgtc ttggggctga
    16261 ccgttacgcc gaacatcacg cccgacggcc aaatcattat gaccgtcaaa atcaacaagg
    16321 actcgcctgc gcaatgtgcc tccggtaatc agacgattct gtgtatttcg accaaaaacc
    16381 tgaatacgca ggctatggtt gaaaacggcg gcacattgat tgtcggcggt atttatgaag
    16441 aagacaacgg taatacgctg accaaagtcc ccctgttggg cgacatcccc gttatcggca
    16501 acctctttaa aacacgcggg aaaaaaaccg accgccgcga actgctgatt ttcattaccc
    16561 cgaggattat gggtacggcc ggcaacagcc tgcgctattg atgcgtcaaa ataagggcat
    16621 atgttttacg gcatatgccc tttctttatg ctttttgccg cgaccgaaat gccgtcattc
    16681 ccgcgcaggc gggaatccag tccgttcagt ttcggtcatt tctgataaat tcctgttgct
    16741 tttcatttct ggattcccac ttttgtggga atgacggcgg aaggggtaaa tcctcgcaat
    16801 ccaaagcctc gtcatttcca caaaaaacag caacccggaa cagcaactta aaaccccgtc
    16861 attcccgcgc aggcgggaat ccagtccgtt cagtttcggt catttccgat aaattcctgt
    16921 tgcttttcat ttctagattc ccacttttgt gggaatgacg gcggaaggga taaatcctcg
    16981 caatccaaag cctcgtcatt tccacaaaaa acagcaaccc gaaacacccc gtcattcccg
    17041 cgcaggcggg aatctaggtc tttaaacttc ggttttttcc gataaattct tgccgtattg
    17101 aaattctaga ttcccgcttt ctcgggaatg acgggatatg ggtttccgtg cggacgtgtt
    17161 cggatttccg cctgcgcggg aatgacggcg acagatgccc aacggtcttt atagtggatt
    17221 aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg gaaccgattc
    17281 acttggtgtt tcagcacctt agagaatcgt tctctttgag ccaaggcgag gcaacgctgt
    17341 actggttttt gttaatccac tatagtattg ataaacatat tatcttcaat atattcaatt
    17401 ggataattgt ttacctaagc aaagataatt gccttttcct gacaaataag tgaaatcaac
    17461 ggattgtcaa aacacagcct gaaataaaaa acctccctga tttcttttat ttttccttaa
    17521 aatcagaaag gttcgggatg gtcgggttat ttttccaaac gtaccgccgc cctgccgatt
    17581 tcgtataaaa ttccgccgta acccgacaag cccgaaccct gtcgccccga aaggcggggt
    17641 gtcaaacatt aaggaattgt gatgaaaaac tttaacggca aactcatcct catcggactg
    17701 atgggcgcgg gcaaaaccac gctgggccgg caaatggcgc agcggctgga ttaccgtttt
    17761 tacgacagcg atcacgaaat cgccgcagcc gccggcgttc ccatccccac catatttgaa
    17821 atggaaggcg aacagggatt ccgttcgcgc gaaaccgcca tactcaaaaa gctggttatc
    17881 ctgccccata tcgtcctgtc caccggcggc ggcgcggtgt taaaagaaga aaaccgcgcc
    17941 cttatccgca aaagcggcac ggtcgtctat ctgcacgccc cgcccgaaac cctgctcgaa
    18001 cgcacgcgct gcgacaacag ccgtcctttg ctgcaagttg ccgatccttt ggcgaaatta
    18061 cgtgaactct acgccgcacg cgaccccgtt taccgccaaa ccgccgactt taccgtagaa
    18121 tcggcaaact gccgggaaac cgtgcaaacc ctgctcaaac gcttatcccg ataaaccggc
    18181 atatgcgccg cgcccagaaa accaagccgc gccccgcccg gcgggccggc ggttcaaact
    18241 ttaaggaaca acaatgaaaa cactgaccgt acacaccccg tcccacagct accccatctt
    18301 tatcggcaac ggactgctgc cgcaggcagg aagcctgctc aaaccgcatt tgggcaaacg
    18361 cgccgccatc atcgccaacg aaaccgtcgc cccgctctac ctcggcacgc ttcagacggc
    18421 attggatgcg gcaggcgtat cccatttcag catcatcctg cccgacggcg aggcgcacaa
    18481 aaactggcag acgctcaacc tcatctttga cgggctgatg caaaaccgcg ccgaacgcaa
    18541 aaccacatta atcgcactgg gcggcggcgt gatcggcgac atggtcggct ttgccgctgc
    18601 cacctaccag cgcggcgcac cgttcgttca aataccgacc acactgttga gtcaagtcga
    18661 ctcatcggtg ggcggcaaaa ccgccatcaa ccacccgctc ggcaaaaata tgatcggcgc
    18721 attctaccag ccgcaggcgg tgcttgccga cttggacacg ctgcacaccc tgcccgtccg
    18781 cgaattgtcc gccggtatgg cggaagtcat caaatacggc gcgctcggcg acatcggttt
    18841 tttcgaatgg ctcgaacaaa acatatccgg actgatgacg ctcgatcggg aaaaactcgc
    18901 ccaagccgtg taccgctgct gccaaatgaa ggcagacatc gtcgcccaag acgaaaccga
    18961 acagggcata cgcgcatggc tcaacctcgg acacaccttc ggacacgcca ttgaaaccga
    19021 gatgggttac ggcacttggc tgcatggaga agccatcgcc gccggctgcg tgttggcggc
    19081 gcgtttgtcc gaacaactgg gcaaaacctc cgccgcagat accgcgcggc tcgccgccct
    19141 gctcgaagcc gccggactgc cgtccgcacc gcccgtgttt gcctttgaaa aatggctcga
    19201 acatatgagc cacgataaaa aagtcagcgg cggcatcatg cgctttatcg gtctgaaccg
    19261 gctgggcgaa gccaacatca ccgaaattac cgacacggac atcctccgcc gcaccctgca
    19321 accgtatctc tgatttcctc tgccgatgtg ctgccgcgcg ggtttgacgc acgatgatga
    19381 ttttccatca tctttctccg taaaagcggg aatccggtcc gttcggtttc ggtcgtttcc
    19441 gataaattcc tgctgctttt catttctaga ttcccacttt cgtgggaatg acggcggaga
    19501 ggtttttgtt gtttcggaga agtttctgca accctagaat ctcgttattt ccacaaaaaa
    19561 cagaaaccca aaacagcaac ttaaaacctc gtcattcccg caaaagcggg aatccggtcc
    19621 attcagtttc ggtcgtttcc gataaattcc tgctgctttt catttctaga ttcccacttt
    19681 cgtgagaatg acggcggaag ggttttggtt ttttccgata aattcttgag gcattgaaat
    19741 tctatagtgg attcacaaaa atcaggacaa ggcgacgaag ccgcagacaa tacagatggt
    19801 acggaaccga ttcactcgtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
    19861 agacaacgcc gtaccggttt ttgttaatcc actataacag caaccctgtc gccgtcattc
    19921 ccgcaaaagc gggaatccag tccgttcggt ttcggtcgtt tccgataagt tcccgttgct
    19981 tttcatttct agattcccac tttcgtggga atgacggcgg aagggttttg gttttttccg
    20041 ataaattctt gaggcattga aattccagat tcccgcctgc gcgggaatga cggctcaaaa
    20101 gttacggaac gaaaaacaac caaaaccgga caagtcggat tcccgcctgc gcgggaatga
    20161 cggaatctta agtttccgtc tttgttttct gttttctgtt ttcgagggaa taacggggaa
    20221 caagccgtat ttcagacggc attttcagtt cggggtataa tccgaatact tgcgaccatc
    20281 tgaatcattg ggacaaacca tgtgtcaact gctgggcatg aactgcaata cgccgaccga
    20341 tattatgttt tcctttgaag gcttccgccg caggggcggc attaccgacc accatgccga
    20401 cggtttcggc atcggctttt tcgaaggcaa aggcgtgcgc ctgttccatg acgacaaacc
    20461 cagtgccaat tcgcccgtcg ccgacctcgt gcgcgcctac cagattaaat cggaaaacgt
    20521 cgtcgcccat atccgcaaag catcacaagg acaaacctcg ctggcgaaca cccatccctt
    20581 tatgcgcgaa atgtggggcg gctactggct gtttgcccac aacggacatt tgattgattt
    20641 ttttcccgaa cagggcgaat ttttccaccc cgtcggcaca accgattccg aacgcgcgtt
    20701 ctgccacatc ctcaaccgcc tgcgcacccg ctttgccgcc cgtcccgacg acgacacgct
    20761 gtttgacgcg attgcggggc tgacgcacga aatccgcaag ttcgggctgt ttaactttat
    20821 gctttcagac ggcattgccc tgtttgccca cgccagcaca ctactgcact acatcgtccg
    20881 ccaagccccg ttcggcaagg cgcgcctgct cgacgacgac gtgatggtcg attttgccga
    20941 agtaaccacg cccaacgacc gcgtcgccgt tatcgccacc ctgccgctga cccgcgacga
    21001 atcatggtcc caacttgccg tgaacgaact ggtcatgttc cgcgaaggca acatcgtccg
    21061 acacgaccgt cccgaaaacc ccgtctatat gagtgccgaa gaaggtctgg aaatcgcccg
    21121 cgccgccggc gtatcggttt gacgttcaga cggcatcagg aagaaacgct atgaaatgcc
    21181 cgttttgcgc ccaccccgac acccgcgtcg ccgattcgcg tctgatggaa gaacgcaacg
    21241 ccgtgcgccg ccgccgccac tgccccaact gcggcaaacg cttcggcacg ctcgaaaccg
    21301 ccgaactcaa aatgcccgcc gtcatcggca cggacaaaaa acgctcgccc tttaatgcac
    21361 aacgcctccg caacgacctg accgccgccg cccgaaaatc cgccctgaca cccgaacaga
    21421 tcgacgaaac cgtccgcctg acggaacaca ggctctacac ttcgggtcag cgcgacatcc
    21481 cctccaccga acttgccgac atggtgctaa aagagctgct tgagcacaac acggaagccg
    21541 ccgtccgctt cgccgccctg cacaaacgct tcgacaatcc gtcagacttt gcctcgtggc
    21601 tggcgcaagc cgtcaaaaca gaccgcaaag cctgattctc cccaacccat actgatacgg
    21661 tatccctatg ttttcggaca cagatatatc catgatggaa atcgccctcc gacttgccgc
    21721 tttgggacgt ttttccactt cgcccaatcc gcgcgtcggc tgcgttatcg cacacggcag
    21781 ccaaattgtc gggcaaggct tccacgtcaa agcgggcgaa ccgcacgccg aagtccacgc
    21841 cctgcgtcag gcgggcgaaa tggcacaagg cgcgaccgcc tttgttaccc tcgaaccgtg
    21901 cagccattac gggcgtaccc cgccctgcgc cgaagctctg ctccgttccg gcgtaacacg
    21961 cgtcgttgcc gccatgcgcg accccaaccc gctggttgcc ggcaaagggc ttgccatgct
    22021 cgaagcagca ggcatcaaga cggaatgcgg tttactcgaa catcaggcaa gggaactcaa
    22081 ccgaggcttc ctgtcgcgca tcgaacgccg ccgccccttc gtccgcctca aatgcgccgt
    22141 ttcgctggac ggcaaaaccg ccctttcaga cggcagcagc ttttggatta ccggcgaaga
    22201 cgcgcgtgcc gacgtacagg ttttgcgtgc cgaaagctgc gcggtgctga ccggcatcgg
    22261 cacggtgttg gcggacaatc cccaactcaa cgtccgcgac ttcccaaccc tgcgccaacc
    22321 cgcgcgcatc gtgttagaca gccgcctgcg cctgcccccg tacagccatt tggttaccga
    22381 cggacaatct ccgaccctta tcgccacctt ggaacgcgat gaagacaaac tccgccccta
    22441 tcgggaacac gcacacatcc gtatcctgat gccgtctgaa acggcagaca gcaaaatcga
    22501 cctgcaccac ctgatgcgcc tccttgctga cgaaggtttc ggcgaaatca tggtcgaagc
    22561 aggctccgaa ctcacatccg catttttggc agaaaatctg gcagacgaaa tcgtcctgta
    22621 ccgttcgccc aaaatcctcg gcagcggcaa agacctgctt tccctgcccg aaaaccgcgc
    22681 cgccctttcc gcaccgccct tgtggacacc cgtttccacc gaaatcctcg gacacgacat
    22741 caaaaccgtg ttccggaaaa acgataacgc cttttaaagg gtttgcgccg tttcactata
    22801 taataacgcc gataaaaaac ggccccgttc agaccgccgc ccgcccggaa aagcgcaacc
    22861 cggactgccg caccccgccg gcagccgcaa cggactgaaa gccgtcaaat tccgatcaag
    22921 aaaggcttca gacggcacag gcagcatccc gccgccgccc ggacatcaaa aatggacaca
    22981 aaagaaatcc tcggctacgc ggcaggctcg atcggcagcg cggttttagc cgtcatcatc
    23041 ctgccgctgc tgtcgtggta tttccctgcc gacgacatcg ggcgcatcgt gctgatgcag
    23101 acggcggcgg gactgacggt gtcggtattg tgcctcgggc tggatcaggc atacgtccgc
    23161 gaatactatg ccgccgccga caaagacact ttgttcaaaa ccctgttcct gccgccgctg
    23221 ctgtctgccg ccgcgatagc cgccctgctg ctttcccgcc cgtccctgcc gtctgaaatc
    23281 ctgttttcgc tcgacgatgc cgccgccggc atcgggctgg tgctgtttga actgagcttc
    23341 ctgcccatcc gctttctctt actggttttg cgtatggaag gacgcgccct tgccttttcg
    23401 tcctcgcaac tcgtgtccaa gctcgccatc ctgctgctgc tgctgacggt cgggctgctg
    23461 cactttccgg cgaacaccgc cgtcctgacc gccgtttacg cgctggcaaa ccttgccgcc
    23521 gccgcctttt tgctgtttca aaaccgatgc cgtctgaagg ccgtccggcg cgcaccgttt
    23581 tcatccgccg tcctgcatcg cggcctgcgc tacggcatac cgatcgcact aagcagcatc
    23641 gcctattggg ggctggcatc cgccgaccgt ttgttcctga aaaaatatgc cggcctagaa
    23701 cagctcggcg tttattcgat gggtatttcg ttcggcgggg cggcattatt gttccaaagc
    23761 atcttttcaa cggtctggac accgtatatt ttccgcgcaa tcgaagaaaa cgccccgccc
    23821 gcccgtctct cagcaacggc agaattcgcc gccgccctgc ttgcctccgc cctctgcctg
    23881 accggcattt tctcgcccct cgcctccctc ctgctgccgg aaaactacgc cgccgtccgg
    23941 tttatcgtcg tatcgtgtat gctgcctccg ctgttttgca cgctggtaga aatcagcggc
    24001 atcggtttga acgtcgtccg aaaaacacgc ccgatcgcgc tcgccacctt gggcgcgctg
    24061 gcggcaaacc tgctgctgct ggggcttgcc gtaccgtccg gcggcgcgcg cggcgcggcg
    24121 gttgcctgtg ccacctcatt ctggttgttt tttgccttca agaccgaaag ctcctgccgc
    24181 ctgtggcagc cgctcaaacg cctgccgctt tatacgcaca cattgctctg cctgacctcc
    24241 tcggcggcct acacctgctt cggcacaccg gcaaactacc ccctgtttgc cggcgtatgg
    24301 gcggcatatc tggcaggctg catcctgcgc caccggaaag atttgcacaa actgtttcat
    24361 tatttgaaaa aacaaggttt cccattatga aaatcgtttt gaccacatct atggcaggct
    24421 tgggcggcac ggaaaccgcc accgtccgcc tcggcaggct actcaggcag cacggacacg
    24481 acatcatgct tgcctcttcg gacggaccgt tgctcaaaga ggcgcaagcc ttgggcatac
    24541 gttggcaacc cgtcgatttc tatcgcggcg gccttttcgg ctacctcaaa ggtatgattg
    24601 cctacgcccg aatgctgcgg cgcgaacaac ccgacatcat cgactgccaa atggcgcgcg
    24661 tcgtacccgc ctgcgccatt gccgccaaaa tcgtttcgcc gaaaaccaaa atcatctacc
    24721 actcgcacgg tttggacgcg gcaacctacc ccaaaatcgc caaactcttc gacaagctgg
    24781 gcgtgtacat catcggtaac tgcaaacacg aacgcgaaaa actcatccgc cacggtttcc
    24841 ccgccgaccg gattgtctac gcctacaacg cact
//
LOCUS       DAWHXS010000016        24240 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWHXS010000016 DAWHXS010000000
VERSION     DAWHXS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.016"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(13..819)
                     /locus_tag="WIE22_000517"
     CDS             complement(13..819)
                     /locus_tag="WIE22_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ4126659.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1040..2410
                     /gene="ffh"
                     /locus_tag="WIE22_000518"
     CDS             1040..2410
                     /gene="ffh"
                     /locus_tag="WIE22_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ4126660.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
     gene            complement(2741..3436)
                     /locus_tag="WIE22_000519"
     CDS             complement(2741..3436)
                     /locus_tag="WIE22_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ4126661.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPVE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(3607..4149)
                     /locus_tag="WIE22_000520"
     CDS             complement(3607..4149)
                     /locus_tag="WIE22_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ4126662.1"
                     /translation="MLHLKDKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFS
                     KTPEAIFRCNLHTELKKKRKLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVV
                     LDVKQKGVDMRIGLDISSITLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWN
                     HVPPDLMEHIDGLKTKCPKP"
     gene            complement(4351..4452)
                     /locus_tag="WIE22_000521"
                     /pseudo
     CDS             complement(4351..4452)
                     /locus_tag="WIE22_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            complement(4655..4825)
                     /locus_tag="WIE22_000522"
     CDS             complement(4655..4825)
                     /locus_tag="WIE22_000522"
                     /inference="COORDINATES: protein motif:HMM:NF012801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4126663.1"
                     /translation="MTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKYWRSDAMPGA
                     PRTYTARVNYSF"
     gene            complement(4830..6767)
                     /locus_tag="WIE22_000523"
     CDS             complement(4830..6767)
                     /locus_tag="WIE22_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4126664.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDAQNNPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLHGSLGVIQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRN"
     gene            complement(6953..7558)
                     /locus_tag="WIE22_000524"
     CDS             complement(6953..7558)
                     /locus_tag="WIE22_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ4126665.1"
                     /translation="MTRQSLQQAAESRRSVYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(7607..8005)
                     /locus_tag="WIE22_000525"
     CDS             complement(7607..8005)
                     /locus_tag="WIE22_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ4126666.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            8113..9024
                     /locus_tag="WIE22_000526"
     CDS             8113..9024
                     /locus_tag="WIE22_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ4126667.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAAGALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            9014..10315
                     /locus_tag="WIE22_000527"
     CDS             9014..10315
                     /locus_tag="WIE22_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ4126668.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPFYTAASPAGLVRLAVRLNTRAV
                     IADESHTFADKLTDNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAYADFDRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLSAPQPAVLSDGIFLPRNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKNTSLMGAYLSAGCISPRLLARKSLERRLNAWTDNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(10383..10607)
                     /locus_tag="WIE22_000528"
     CDS             complement(10383..10607)
                     /locus_tag="WIE22_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126669.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLMIESEKNGRY"
     gene            10710..12860
                     /gene="dinG"
                     /locus_tag="WIE22_000529"
     CDS             10710..12860
                     /gene="dinG"
                     /locus_tag="WIE22_000529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ4126670.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNMAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            12896..13510
                     /locus_tag="WIE22_000530"
     CDS             12896..13510
                     /locus_tag="WIE22_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ4126671.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGQATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
     gene            complement(13801..15669)
                     /locus_tag="WIE22_000531"
     CDS             complement(13801..15669)
                     /locus_tag="WIE22_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ4126672.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSHGGALQ"
     gene            complement(15750..17120)
                     /gene="purB"
                     /locus_tag="WIE22_000532"
     CDS             complement(15750..17120)
                     /gene="purB"
                     /locus_tag="WIE22_000532"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ4126673.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(17192..17824)
                     /locus_tag="WIE22_000533"
     CDS             complement(17192..17824)
                     /locus_tag="WIE22_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ4126674.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
     gene            18133..20013
                     /locus_tag="WIE22_000534"
     CDS             18133..20013
                     /locus_tag="WIE22_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ4126675.1"
                     /translation="MNIFYEESGQFKIAAIVQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
     gene            complement(21247..22416)
                     /locus_tag="WIE22_000535"
     CDS             complement(21247..22416)
                     /locus_tag="WIE22_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ4126676.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKAAKA
                     AKAAKAAKAAKAAKAAKVAKVAKVAKVAKVAKVAKVAKVAKVAKVAKVAATAQKEAAP
                     AQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESELSRQVLMQLV
                     NQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPAQRRELADNIIAEKVRQQAVMQ
                     NSRVGEAEVDRFIEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAVGAESTIRKIY
                     GEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPVRT
                     QFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHSGAYVDIR"
     gene            complement(22521..>24240)
                     /locus_tag="WIE22_000536"
     CDS             complement(22521..>24240)
                     /locus_tag="WIE22_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ4126677.1"
                     /translation="HAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLS
                     VPYYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNR
                     YQAKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAA
                     GGSLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHD
                     SRQDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNI
                     DSGATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENL
                     YYGNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRN
                     RSDWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNE
                     KIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSC
                     GCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSA
                     GRNKRP"
BASE COUNT         5738 a         6591 c         6370 g         5541 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcatcagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcaaaaa atgttccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagttcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccaaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccat ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccga gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctaatctat
      841 actgttgcgc tgtatgcggc cgcttatgaa atattggaac ttttaacgtt ggaattgtaa
      901 aatccccgtt tcgggcaagc cttgacggat ttgccgatat gctgtccggc acacaagccg
      961 catcaaatta ttttgatttt attttaacaa agaatgcccc tgatggggca agctattctt
     1021 attcagacca aggaccagta tgttagacaa tttaaccggc cgcttcagca atgttctcaa
     1081 aaacatccgc gggcaggcca aactgaccga agacaatatt aaagaagcct tgcgcgaagt
     1141 ccgcctcgcc ctgcttgagg cggatgtcgc cctgcctgtc gtcaaagagt tcgtcaacaa
     1201 cgtcaaagaa aaggccctcg gtcaggaagt ggtgggcagc ctgacgccgg atcaggcatt
     1261 tatcggcgtg gtcaacaaag ccctgaccga gctgatgggc agggaaaaca aaacgctgga
     1321 tttgtcggtt gcgccgcccg ccgtcgtgtt gatggcaggt ttgcagggcg cgggcaagac
     1381 gacgaccgcc ggcaaactcg cccgcctgct gaaaaacgat cagaagaaaa aggttttggt
     1441 cgtatctgcc gacgtttacc gccctgccgc gattgaacaa ctgcgtctgt tggccgaaca
     1501 ggtcggcgtg gactttttcc catccgatac caaccaaaaa ccggttgaga ttgcaactgc
     1561 cgccgtcgat tacgccaaaa aacattttta cgatgtattg atggtcgata ccgccggccg
     1621 tttggcaatc gatgaggaga tgatgaacga aatcaaagcc cttcacgcgg cggttaaccc
     1681 ggtggaaact ttgttcgtca tcgatgcgat gctgggtcag gatgcggtga acactgctca
     1741 ggcatttaat gaagccctgc cgctgaccgg tgtcgtattg accaagatgg acggcgattc
     1801 gcgcggcggc gcagctttgt ccgtgcgcca cgtaaccggc aagccgatta aatttatcgg
     1861 tgtcggcgaa aaaatcaacg gcctcgaacc tttccacccc gaccgtcttg ccagccgtat
     1921 cctcggcatg ggcgacgtat tgaccctgat tgaagacgtc caaaaaggta ttgatgaaga
     1981 agccgccgcc aaaatggcga aaaagctaca aaaaggcaaa ggcttcgacc tcaacgactt
     2041 taaagaacaa atccagcaaa tgcgcaatat gggcggtttg gaaaacctga tgtcgaaaat
     2101 gccgggcgaa ctgggtcaaa tatcgaaaca aatccccgaa ggaacggctg aaaaagcgat
     2161 gggcaaagtg gaagccatca tcaactcgat gactccaaaa gaacgcgcca accctgccct
     2221 gctcaaagcc agccgcaaac gccgtattgc aatgggtgcg ggcacgaccg tacaggaagt
     2281 gaacaaattg ctcaaacagt ttgaacaaat gcaacaaatg atgaagatgt tcagcggcaa
     2341 cggcttgggc aaactgatgc gtatggcgaa aggaatgaag ggaatgaaag gaatgttccc
     2401 gggtttgtaa gccgatttga ccgaaaacgc cgtctaaaat ttcagacggc gtttttgttt
     2461 tatattctga tttatagtgg attaacaaaa atcaggacaa ggcggcgagc cgcagacagt
     2521 acagatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
     2581 agctaaggcg agccaacgcc gtactggttt ttgttaatcc gctatatatt ctgatttaaa
     2641 accataaggc tttaagcaat catctcttct ataaagccta aatacaaaag gccgtctgaa
     2701 atcctatttt cagatggcct ttactcattc aatcctcaac ttattgcggc tttttctgct
     2761 cttcgcgtac tttatccacc aactggtcaa tcgtggtcat accggactgc cagtctttaa
     2821 attcaacttg gtatttgccg ccgacaatca cagtcggtgt gccgctgatt tggaatttat
     2881 tggtcaactc ttccatttga gccgcacgcg cttggctttc aggagcctca aatgcagcca
     2941 atactttttt gccgtcaaac gctgtttgct cggacagcca ttttttcagg gtatcggtat
     3001 cggccagatt gattttttga ttaaccatcg catcgaaaat atggctgttg gctttatctg
     3061 attcaccggc catttccact gcggccgcca aacgtgccaa aggtttcatt tcatcacccc
     3121 acacgacatg ctcccggcgc atataggtat cgtctttaaa cgttttgatg tgctcgctca
     3181 agaccggctc aagatgggcg caatgcgggc agaagtagcc gaaaaattcc aatacttcga
     3241 ttttaccggc ctgctgttgc ggaataggcg tagacaatac agtgtagttc acaccttcgt
     3301 tcaactcaac aggggctgcc ggagcagatg agctgctttg gacgctgtct gccggaacgc
     3361 tggtttcagc ctgtttgcta caagcggcca atgccaacag ggtcaatgaa gtcaaagcta
     3421 aggttttcag tttcataggt atctcttgtg tgtcagatta atgtgcggat tttatggcat
     3481 tttattgaag gcgtgttcga tttgagtgaa aaaatgtgtt tagttttatt tcagccttta
     3541 taaaggccgt ctgaaatatg gcttagataa ttagccttat tttcagacgg cctttgttct
     3601 agtaatttac ggtttcggac attttgtctt caatccgtcg atgtgttcca ttaagtcagg
     3661 tgggacatga ttccacatag gatcaagaat aaaatcgata ccttcccgtc tggctaattt
     3721 ggctgctggg acaaaatcgg aatcaccaga aaacaagatg attttatcgg cttgtttttt
     3781 taaggtaata gatgaaatat ccaagcctat acgcatatct acaccttttt gtttaacatc
     3841 gagcacgaca tcgttttccg taatttgagt tgacgggttt ttcagaagat ttttcatgac
     3901 ttggggtttt aatatccatg ctgtattgtc cgacagcttg cctaaacgta acgctaattt
     3961 acgcttcttc ttcaattcgg tatgcagatt gcaacgaaat atggcttccg gagtctttga
     4021 aaaatctatg gcacgatgag atacgggatt gtgcattttc ttttcaaacg gcacgcagtc
     4081 gtaaaagaaa attctatata aatcatccat ttgatctgtg ccgttgattt tatctttcag
     4141 atgcagcatc gaatatcgga aagccaaatc gagacctttg caataacata ggttactaaa
     4201 attttatgct caatctcatt ttcaaaatgc aaaacttttc tgatttttcc tactttttgc
     4261 tcaatattag gaaagtttta ggcaattgaa aattttttgg cgcattttta tgcgtcaaat
     4321 ttcgttaaca gactattttt gcaaaggtct caaatcagca attctttttg catcgtgtgg
     4381 attttgagac aggcctaatg accgtacccg cttaataaaa aatgcaccgt caatcaaaat
     4441 ggcggttttc atattgcttc ccctatattt gtcaaagata taaaaaagcc cttgggatcg
     4501 gcatacccgc tatgatttaa tgggtaatgc gtactgccaa gggcgctatt tctttttgga
     4561 ttatactgat gtagataaag cctgtcaaaa agttcgtgtg ctgaatttac ggattatcag
     4621 gattttcaga cggcattctg ttgcacgaac gggattagaa actgtaatta acccgcgccg
     4681 tataagtgcg cggcgcgccg ggcatggcat ccgaacgcca atatttttga ttgagcagat
     4741 tggctgcggc aaaggtaatg ttaacatttt tatggttcca gccaagcatg gcatcaactc
     4801 gggcaaagcc tggaagcgta gtcacttctt taatttcttg agttgtaacc gtagcgtttg
     4861 cctgtaccgg ttacgccgat ttcgccgtag aggttttcgg tcggggtata acggaaaaac
     4921 aggttgccgg taacgttgct ggtgttattc aaatggatgc ccactcggtc gggattttct
     4981 ttgtcttcaa cgactttcgc ctgaatcacg cccaacgaac cgtgcagata gagttttttg
     5041 gggatgattt gcccgatggc ggacaattcc acgccacgcg aacggtgttt gccgccgact
     5101 gcccaagtgt agggattatt ttgtgcgtcg gggcggtagc ggatattgaa gcgttcgatt
     5161 tggtaggcgg acaatgtggt gctgaggcgg tcgtccagcc aactgctttt aacgccggtt
     5221 tcgtattggc gggtgtactc ggggtcagcg ttgaacacgg cagaagatga cgtatcgatg
     5281 ctcaaatagc cgccgcgtcc gccataaggc gcgaaacctt tgttatacga ggcgtaaagt
     5341 gtgtgaacgg ggttgatgtt ccacactgcg ccgatgttgg ggctgaacga gtggccgctg
     5401 tactggcggc tgctgccggt gagtttgttt tcggaattaa aggtgtattt gtcgtaacgg
     5461 ccgccgagga cgaatttcaa atcgggcgtg gcggagaaga tgttttgcac aaagatgccg
     5521 taggagtcgg ctttgtggcg gttttgggtg aggataggct gcaatctgcc cgaagccggc
     5581 cagcttgctc ggtcgtatgg atcgatggaa gcagtaaagg cgcggctgta acctaaggtc
     5641 gggttgcggt gttcgcggct gtaatccatg cctacggtca agtggttttc aaaacggccg
     5701 atggtgtagt cgccgtttag cgtgaaattg gacgacaggg ttttgttgtc ggtctgctgc
     5761 caggcgtagt tacgtttgat taagttgcca ttttcgctgc ctgcatagaa atgatcaaaa
     5821 tcctgcgccg ccgtgcggtg ggcgagctgc cattgggcac gccatttgtc gttgaaggcg
     5881 tattcaaggt cggagcgcca aacttgcagc ttgtctttga caaaatcgtt ccggtgggcg
     5941 aaccccatgc ggtaaggcag tccgaagcgg tcgtacacgg acttggtcgg actgcggtcg
     6001 ggcgtgcgct ccacattgtc gtaggtgtat tgccccgtcc acttcaagcc gttgtcgagt
     6061 ttgacggtaa tgctgggcga aaccatgaca tttttgctgt ctatgccgct gcggaacgaa
     6121 ttggcgcgcc cgacttcgcc ggtgagacgg atggcgacgt ttttgttcaa cacttcgtta
     6181 atgtccatat tcaggctgcg gtttgcccac gaaccgtaaa ccgcaccgat gttgcggctt
     6241 tgtttgaagt tggcgtattt gctgaccatg ttgatgacgc cgccgccgtt ggtgcggccg
     6301 taaagcacgg aagacgggcc tttcaggatt tccacgcgct cgatgttggc agtactgcgg
     6361 cgcacttgtc cgctttcgcg cacgccgtcg cggtaaatat cggatgcgtc ggcttgaaaa
     6421 ccgcgcagga aaatgctttc gccgcgcata tcgtaggcag cgtcgatgcc ggcattgcct
     6481 tcgaggatgg aactcaaatc gttcgtaccg taatttttgt ttttctggat attgagcgta
     6541 tcgatggttt gcggcgtttc tttgatgagc tgtccgttgc gggtaacggc ggcttcgtcg
     6601 tagttgatgt agcctttgag tacgctggtg tcggactgtc cgaccacgga aacggtgggc
     6661 agagtggcgg tgtaatgttc accattgtcc tgcgtatcgg cggcagcaac agggaaggaa
     6721 gcaataatca gcgtgggtaa taaagctaaa tgaaatgata ttttcatttt tatactcaat
     6781 ttaacaaaac aaccgaatta tattgcctca cggaggaaat gagaataatt tcttttaact
     6841 atattgaaca tgatatttgt aaacaaaggt ctcagaatgc ggaaaactcg ccgcctgata
     6901 ctgaaaaatg ccgtctgaac agggttcaga cggcattttt ttgaccgcga aattatgcgc
     6961 cgaacacttt caaacgttct gcaacgggtt caaaggtctt ttcacctgcc gccccttcaa
     7021 taccgccgat aaccatttgt gcgcgcaaca accagttttc ggggatattc cacgctttgg
     7081 caatcgccgc atcgggcaag ggattgtaat gttgcaggtt tgcacctacg ccgaccgcgg
     7141 caagtgtcgt ccaaacggca tactgcacca tagcgttcgc ctggtccgcc caaacgggaa
     7201 agttggcggc ataagcaggg aactgctcct gcaaaccttt gacaacattt tgatcttcat
     7261 aaaacaaaat agttgccgca cccgccttaa acaggttcaa tttttgcgcg gtcggttcaa
     7321 aactgtcggc aggcacgacg gcacgcagcg cgtcttcgac aaattgccac accttatcat
     7381 gctcttcgcc aaacagcacg accacgcggg cagattggga attgaacgaa gaaggtgtgt
     7441 gcaaaacggc gtgttcgacg atttggacaa cttcatcttt gccgacgggc aggtttttat
     7501 ttaacgaata aacggaacgg cggctttcgg cagcctgttg cagagattga cgggtcattt
     7561 cttttcctca aaaacattca acaaaataca ctttaggcag tccggttcag gctttacgct
     7621 tgaaaaacag cctgtccagc gaccatgctc cgccgcccgc tgccgcgata tagaggaata
     7681 cgaagcagaa cagcactgcg gactcgccgc cgttggcaat cgggaacaaa gcatttccgg
     7741 aagcgtgcgc cataaaatag gcaaccgcca tctgaccgga caaaacaaac gcggcagggc
     7801 gcgcaaacag gcccaacacc agcaaaatgc cgccgacaat ttctaaaata ccggcaagca
     7861 gcaacagccc gccgggcgaa ccgctgccca tttcaatggg gaaggcgaag attttcgacg
     7921 taccgtgcaa caaaaacagg taggcggtta cgatacgcaa aacagaaagc aaaaccggtt
     7981 ggatacggtt gcagcaatcg gacataaaag ctcctttgat atttgtcttc atttcaggcg
     8041 gtagagtata gatgtttccc cttttcaaat atatactgct tttattgata caacttttcc
     8101 acacaagaaa atatggacac cctgttcagc ctcaaggttt tccgccaagt cgtccaaagc
     8161 ggcggcttca cccgcgccgc cggcgcgctc ggcatctcca ccgcaatggc aagcaaacac
     8221 gtcagccact tggaaaacac cgtccaagcc aaactcctgc accgcaacag ccgcaacctc
     8281 agcctgaccg aagccgggga agaatactac cggcaatgca gttacgcgct cgacacgctc
     8341 gacgatgccg cgcaaaaagc cgccgggggg acggaaaaac cgcaggggct gctgcgcgta
     8401 acgatgccac tgtggtttgc cggcagccag atatgcaact ggctggcgga ataccgcgaa
     8461 cgttatcccg aagtgacatt agaactgatt ttggacaacc gccacgtcga tttgattgcc
     8521 gaaggcgtgg atttggcgtt gcgcgtttcc caaaccctat ccccgtcact gattgcgcgc
     8581 ccactggcgg aaatcgaatt tgccctgctt gcctcgcccg acttcctgag gcgcaacggc
     8641 gtgccggaaa cgccggaaga agtggcgggg ctgcccgccg tcctgccgac ctacaccaac
     8701 cagcagaaac tcgacctcac ccgcaaatcg gacggcaaaa aataccggct tgaactgacc
     8761 cccgtcatcc gtaccgacaa cacgctgatg atgcgcgaaa tgattaaggc gggcgcgtgc
     8821 atcggttatc agccgctttg ggcggcggaa cacgatttgc gctgcggcac gctggtgagg
     8881 ctgctgcccg gatacgccgt cccgaccgac cggctgaatg ccgtttatgc agacagggca
     8941 ttcttaagcg cgaaagtccg cagcttcatc gattttctga acgaaaaaat cgccagcagg
     9001 aaaggctgcc gaaatgccgt ctgaaaccgc ccgccccctt tatgcggaca cgtgcgccgc
     9061 acacacgctc gtctggttcc gccaaaacct ccgcatccgc gacaacgccg ccttatgcgc
     9121 cgccgttgcc gaaggtttgc ccgttatcgg tatttggatt gacgatgccg aaacagacaa
     9181 ccctcgccgc gccgcgttct accgccaatc cgccgccgaa ctcgcccaag ggcttgcagg
     9241 gcgcggcatt ccgttctaca cggcggcatc gcctgccggg cttgtccggc tcgccgtccg
     9301 cctcaatacc cgcgccgtca tcgccgacga atcccatact tttgccgaca aactcaccga
     9361 caacgccctt tggcatgagc tggacaaaca cggcatcgcg ttaaccttcg tcaacgaccg
     9421 cgccgttttc ggcaaaaccg acctgatacc cgacggcggc acggcatatg ccgatttcga
     9481 ccgctaccgc gaagtatggc tcgaccgctt ttccaagcag ccccccgccg gttcggacct
     9541 attcgcggca taccgccaac ccttccccga aaacctttcc gccccgcagc ctgccgtgct
     9601 ttcagacggc atcttcctgc cgcgaaacgg cggtgaaacg gcggcttggc ggcagtggcg
     9661 gcggtttctc gaacaggcgg attcctactc cgtcttaaag gatttcccct cgcgcaaaaa
     9721 cacttcgctg atgggcgcgt atttgagtgc cggctgcatc tcgccgcgcc tgctcgcgcg
     9781 gaaaagcctc gaacgccgtc tgaacgcgtg gacggacaac atcatccgcc gcgatttttt
     9841 ccttcaactt gccttgcagc acacggatga cgacccttca gacggcaatc ctgaacacac
     9901 cctgcgcctg acgctttggc agcagggccg gaccggcatt ccgattatcg atgccgcgat
     9961 gcgctgtttg cacaaaaccg gcagcctcca ccccgccctg agacgcttga gcgcggattt
    10021 tttctgccac gttttaaacc tcccccgccg cgaaggcgag atatggtttg cccggcagct
    10081 gaccgatttc gatgcagcaa tcaaccaagg caactggcgg cttgccgcct cacggcacac
    10141 ctgccccgac attgccgccg ccgcacacaa aaccgacccc gacggcacct ttgtcagacg
    10201 gcacattccc gagcttgccc acctgtccgc cgacaccgtc cacacgcctt ggcggtttgc
    10261 ctgttcggtc gatacccacg gctatcccgc ccatcctgtc gccggtattc cctaagcggc
    10321 acggcaacct aaaacaaatg ccgtctgaac ttccgtttca gacggcattg cgtccgaatg
    10381 actcaatatc ttccgttttt ttcactttca atcatcaggt atgcccaaat caccgccacc
    10441 caagccgaca ccgttcccat cgccggcaaa aaatgctgcc aatccgtcat cggcggcaca
    10501 gggcaacggc cgcattcgat ttccgcgccg cccaacaacc ggtacagcaa catatccgca
    10561 atccaaagca acaacgggta tttcaaacgc tgcaaccaaa aaaccaacca cgtccgcacg
    10621 attcacctca ccccccattc cgacaacgat attgtaccgc ttccgtgcaa tgttaaaata
    10681 agcccacttc taccgccgta caaaacgcca tgctcaccga tttagaaaaa aacgccatcc
    10741 gcgaccatta ccaaaacatc ggcaaaaacc tgcccggttt ccgtccgcgc gcttcgcagc
    10801 gggaaatgat tgcggcggtt gccaacgctt tttcgcggac gttggcgcgc gaagaaggcg
    10861 gcgagccgcc caagcgcgag ggcgagagca tcgccgtaat cgaagggccg accggcgtgg
    10921 gcaaatcgct tgcctacctg ctggcgggcg gcatcatggc gcagacgcgc ggcaagcggc
    10981 tgattgtgag cagcgcaacg gttgccttgc aggagcagtt ggtagaccgc gacctgccgt
    11041 ttttggtcga aaaaagcggt ttggaactga gcttcgcgct tgccaaaggg cgcggccgct
    11101 atctctgccc ctacaaactc tatcaactga cgcaaagcaa tgcccagcaa aacctgctcg
    11161 gctttgaagc ccccgccgtc ttgtgggaca gcaaacccaa gcccgaagaa ttgaagctgc
    11221 tgcgcgacat tgccgacgaa ttttctgccc gacggttcaa tggcgaccgc gacgcctggc
    11281 cggaaaaaat cgacgacgcg atttggctca aagtgaccaa cgaccgccac ggctgcctga
    11341 aaaccgcctg tcccaaccgt ccggaatgtc cgttttacct ggcacgcgat gtcttggaaa
    11401 ccgtcgatgt cgtcgttgcc aaccacgatc ttctgcttgc cgacatcagt atgggcggcg
    11461 gcgtgattct gcctgcgccc gaaaacagtt tctattgcat agacgaagcg caccacctgc
    11521 ccaaaaaagc cctcagccgt tttgccgccg aacattcatg gaatatggcc gtttggacgc
    11581 tggaaaaact gccgcagctg accggcaaga ttgccgcgct gaccgataaa gccgaacttg
    11641 ccaaccttgc cgacgaagcc gcctcatcct tactcgacag cctgcacgaa tggcagttcc
    11701 atttggcgga agagccgtct ttgagcttgg ggccgtctga aaacgacagg cggaccaaca
    11761 gcgaaccgac ttggctgtgg gaagacggta aaatccccga aggcctcgaa accaccgttt
    11821 ccaatacggc cattgctgcg cgcagcctgc tcaaacacgt tgtcgggctg aacgatgcgc
    11881 tttccgccgc gcgccgcgaa aaagaacagg acggcgcgct cctcgaccgc ctgaccggcg
    11941 atttcggcat cttcatcgcc cgtatcgaac aaatcagcgc ggtttgggat ttgctctcta
    12001 ccgttcccat tgagggcgaa gagccgttgg cgaaatggat aacccgccgc gccgacgaca
    12061 aaaacgacta cattttcaac gccagcccca tcagcagcgc gtcccacctc gccaacagcc
    12121 tgtggcggcg tgcggcaggc gcggtgttga cctccgccac cctgcaatcc ttgggcaact
    12181 ttaacctgat gctgcgccaa accgggctgc aatggctgcc tgaaaccacc accctcgccc
    12241 tcaaaagccc ctttgacttt gaaaaacagg gcgaactcta catccccccc atatacgcca
    12301 gccccaaaga ccccgaagca cacaccgccg ccgtcatcga gtggctgccc aagcttattt
    12361 cgcccaccga agccatcggc acactcgtcc tgttttcctc gcgcaaacaa atgcaggatg
    12421 tcgccctgcg cctgcccgga gactacctgc cgctcctgct cgtacaaggc gaattgccca
    12481 aagccgtcct cctgcaaaaa caccaccggg ccatagaaga aggcaaagcc agcatcatct
    12541 tcggactcga cagctttgcc gaaggactcg acctgcccgg caccgcctgc gtgcaagtca
    12601 tcatcgccaa actccccttc gccatgcccg acaaccccat cgaaaaaacc caaaaccgct
    12661 ggatagaaca gcgcggcggc aaccccttca tcgaaatcac cgtccccgaa gccggcatca
    12721 aactcatcca ggctgtcggc cgcctcatcc gcaccgaaca agactacggc cgcgtaacca
    12781 tcctcgacaa ccgcatcaaa acacagcggt acggccaaca attattagcc ggcctgccac
    12841 cgtttaaaag gatagggtaa acctaccgcc cttcccgcaa cagaaaggaa aagtcatgaa
    12901 cttcacccga ctgctcaacc aagtcttaag cacggttcaa aaaaaaggca acacattctc
    12961 cgacagcccg ctcaattcat tcggcggagg cgcgctggtt gccggtgtcg cctccatgct
    13021 actgaacggt aaaaaccgca aaaccatcac caaaatcggt tcgaccgccg ctttgggcta
    13081 cctcgcctac cggggctatc agatgtggca gcaaaacaaa gggcaggcaa ccgtaacaca
    13141 aagcgatttc caacctgccg gagaaactga agaaacatac agccgcaccg tattgcgcac
    13201 catgatagcc gccgccgctt cagacggcat gatagacgaa gccgaacgcc ggactatcga
    13261 acaggaaagc ggcacagacc ccgaaactgc cgcatggctc gccgccgaat accgcctgcc
    13321 cgcaagcatc ggggacatcg ccaccgccgt cggcaacgat gaggcgttgg cggcggaggc
    13381 ctatctggcg gcaaggttgg tctgtgccga tttgtcgcgg aaagaaaccg tcttcctcgc
    13441 ccgcctgtcg caggctttga aactggatga caatctggtg gagagtttgg aaaggcaatt
    13501 ggggttttga tgacacagct ggggagggaa aatcatcaaa aaacggttat tccgaccccg
    13561 gcctcaaaac ggcaaaatat agtggattaa caaaaaccag tacggcgttg cctcgcctta
    13621 gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt
    13681 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta taaaagccgc
    13741 cgccccgaac ggcaaacagc ggcataggct gccaaaggca atatccgaca agaaggcagg
    13801 ctactgcaat gcgccgccgt gggaagattt aagcaggcgt tcgtgtttgt ggaattcccg
    13861 ccccatataa taagaaccga aataggtcag gtggtcttgg tcgccgtaaa gatagcgtcc
    13921 gtgtatttcg accgtgtttt tgggcaggta tttttgtgcg tccacccaat gcacattggg
    13981 gatatcttta accaaatcaa aaaccgcctg attgctcttg ccgatgtcgc ccatagcctg
    14041 aatggggcgg agatattggt ttgcggcaaa tcttttcaat ttttcctccc tcaggggcga
    14101 acggctgatt gatgtgttgt ttgcaaaaac atagacgggt ttgacggcgg ctatcctttt
    14161 gacggtttcc ctgaatcggg cttggaaccc gggtattagg aaggattgcg cttcaaatct
    14221 cggcacgggc tggccgccca tcctcaaatc atagaattgg gcaatgaaaa cggcttcggc
    14281 tttttcaact tcatcccggt attttcgaca taacgggttg tctgccagct tctcatctac
    14341 ccaaaccaaa cactccgaat cgagggacag gattttggct ttccaccctt cccggctgcc
    14401 gacataatcc agaaaccccc gcaggtgtcc ggcgtgcgag tcgccgaggg tcaggacggt
    14461 ttccggaaaa tgattttccg cagcaagggg cgcgccgggc aacgggcgga ggtgttcctg
    14521 tttcaatatc ccccttgcgt acaggttgta accgacaagt atcagggacg gggcgagata
    14581 gaggcagaaa aatgcctttt tgaaggtcat cttccgtttt ctaagcggct gttcaatcaa
    14641 ataataactc aacagggaaa atccggccgt caacgcggca accgccgata cggcaggcag
    14701 tccgagctgt ttgtcgcctg taatgtaatg ggcgaaagca ataaaaatcc aatggtacag
    14761 gtatagggaa taagagattt tgccgacaaa tacgatgggg cttgccgaca ggatgcgggt
    14821 cggaagtgtc ccgtattgca tactccggat aagcagtgcc gtcagcaggc agggaaggag
    14881 cagggtcatt cccgggataa acggattgtg tttgtcaatc acgaacaggc aggcaagcaa
    14941 tgcgccgaag cagagtgatg aaagcaactg ccgttttcca tttgctgttt gccgtctgcc
    15001 gttttgcgtt tgcccgtaaa ccgccagcag cgaacctgcc aacagctcgg gaaacctcag
    15061 tgtcgaaagg taataagtat tgggttggtt gagaatatcg gtataaaacc cgcttggcaa
    15121 aaacgatgtg gcagtcaaaa tcagaaatag gatgatgctg atgttacgca gcacccgtag
    15181 cgattttgtt tttttgcagc aaaatatcag caaaagagga tacaggaggt aatactgttc
    15241 ctctactgcc aaagaccaga tatgcagtac ggggttctcg tcggcactca aatcgaaata
    15301 cccctgctga aaccccagat aaatattgga caagaaaacc gcagaaagct ccacggtttt
    15361 ccgcatttgg ttgaaatctt cgtaaaggaa gatttgagag gcaatcaccg aagccagcga
    15421 cacggccgca ataaaagcag gataaatccg cttaatcctg cgggtataaa aatcccggaa
    15481 agaaaaagaa ccgttctgta tttcagaaag aatgatgccg gtaatgagga atcctgagat
    15541 gacaaagaaa atgtccaccc ccaggaatcc tccgggcagc cagcggttat tcaggtggaa
    15601 aatcatgacg gatagcacgg cgacggcccg caatccgtca atttccggtc tgtatcggac
    15661 agcttgcata aatatcgccc ccgtatgtcc cgttatctta aaaatgccgt ctgaacgcgc
    15721 gttcagacgg catcggtttc agtaaacgct caaatccgtt tcgccaacgc ttcagccttg
    15781 cccacataca gcgcgggggt cagctcaagc aatttggctt tggcttcggc ggggatttcc
    15841 agcaatccga taaagccttt cagcacttcg ggcgtgatgc cgcctttgcc gcgcgtcagg
    15901 tctttcagtt tttcgtaagg attggcgaca ccgtaacggc gcatcacggt ttgaatcggc
    15961 tcggcgagca gttcccaagt ggcatccaaa tcagcggcaa gcgcggcagg gttgggttcg
    16021 agcttgttca gaccgcgcag gtgggcggcg aaacccaaca cggcatagcc cacgcccacg
    16081 cccatattgc gcaatacggt gctgtcggtc aggtcgcgct gccagcggga aatcggcagt
    16141 ttttcggaca aaaagcccaa tacggcattg gccataccga ggttgccctc ggagttttca
    16201 aagtcgatgg ggttgacttt gtgcggcatg gtcgatgagc cgacttcgcc cgctttgact
    16261 ttttgtttga agtaacccaa tgaaatataa ccccaaacgt cgcggttaaa gtcgatcaga
    16321 atcgtgttga tgcggctgag ggtttggaag aattccgcca tatagtcgtg cggctcgatt
    16381 tggatggtgt aggggttgaa ggtcagaccg aggctgattt cgacgaagtt gcggcagtgg
    16441 gtttcccaat ctacatcagg ataggcgacc atatgggcgt tgtagttgcc gaccgcgccg
    16501 ttgattttgc cgaggaactc ttgcgcttgc aggtttttaa actggcgttg caggcggtac
    16561 acgacattgg cggtttcttt gcccaaagtg gtcggcgtgg cgggctggcc gtgggtgcgg
    16621 ctcatcatcg ggacggcggc aaggtcgtgc gccatagcgg tcagtttttc gatgatttcg
    16681 gccagcttcg gcagcaaaac agcctcacgc gcttcttgca gcattaaagc gtgggacagg
    16741 ttgttgatgt cttcgctggt gcaggcgaag tggatgaact cactcacggc ggcgacttcc
    16801 ggcacttcgg caaaacgttt tttcagccaa tattcaatgg ctttgacatc atgattggtc
    16861 gtggcttcga tggctttgac ggcggccgcg tcttccaatg aaaagttttc aatcaccgtg
    16921 tcgatttcgg caagcgtttc ggcactgaag ggcggcactt cggcaatctt cggctcggcg
    16981 gcgagggctt tgagccagtt taattcgact ttgacgcgcg ctttcatcag gccgtattcg
    17041 gaaaaaatcg ggcgcaatgc ttcaacggat tgggcataac ggccgtctaa aggggaaagc
    17101 gaggcgatgg ggttgatcat ttcggcatcc tgttcgggaa agacatcaaa aattgttaaa
    17161 ttgtttgcaa ttatacatca ctctcaggac gctatgccgt ctgaagcccg tgttccgaca
    17221 gatacgcaac gatttcgtct tcatcgtccg atatggcgaa caaagaggct gccccttcgg
    17281 aaatcagacc gcgcgccaaa agctgcgcgt ttatccactc cgccaagccc gaccaaaacg
    17341 cctttccgac caaaacaatc ggacgcggcg gcactttgcc cgtctgcacc aaggtcagga
    17401 tttcaaacaa ttcgtccagc gtcccgaagc cgcccggcat cacgacatat gcttgggaat
    17461 agcggaaaaa caccgccttg cgttcggcaa aacgggaaaa ccgcaaggcg atgtcctgat
    17521 acggattcgg tttctgctcg tgcggcaaaa cgatgttcag ccccaccgaa accgacttcc
    17581 ctgcaaacgc gcccttgttt gccgcctcca taatccccgg cccgccgccc gaaatgacgg
    17641 caatgcccga atccgacagc cgccgcgcca gacggcaggc gaacgcataa tccgcatgat
    17701 tctgcggcgt gcgcgcgctg ccgaagatac tgactgccgg aaacacgccc gccaatgctt
    17761 cgtctgcctg cctgcgttcg gcatcataac gtgcctgctc cggcacacgg tttgtattct
    17821 ccattccatc ctccgttcaa aaacagcgat tgtacaccgt caaaaacgta tagtggatta
    17881 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca
    17941 cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg taacgctgta
    18001 ctggtttttg ttaatccact ataacgcaag caccgcaaag acgcgccaac cctctcccaa
    18061 ccttttttca gacggcattt tcggtaatct gctaaaatcg cccgcttgag tttccacaga
    18121 aaaatccgaa aaatgaatat tttttacgaa gagtccggcc aattcaaaat cgccgccatc
    18181 gtccaaaaaa acgacgccac ctaccaagtc gataccccac acggcaaacg caccaaagtg
    18241 aaggcgaaca acgtctttgc cgagtttgac ggcgatatgg cggcgttttt ggaaaacgcg
    18301 caggcacagg cggcggacat cgacaccgat ttattgtggg aagtatgcgg cgaagaggaa
    18361 tttaccgccg aagccatcgc cgaagaatat tacggccatg cgccgaccaa aaccgagctg
    18421 gcggcaactt tgattgcgct ttacgccgcg ccgatgtatt tctacaaaaa agccaaaggc
    18481 gtgttcaaag ccgcgcccga agaaacttta aaacaagcac ttgccgccat cgaacgcaaa
    18541 aaacagcaag acgcgcaaat cgacgcttgg gcggaagcct tgaaacgcgg cgagatgccg
    18601 tctgaaatcg cggcggattt gaaaaccatc ctgcacgcgc ccgacaagca gtcgctgacc
    18661 tacaaagcct ttaccaaagc cgccgacgcg ctgaaaacct ctgcctacga attggcgaaa
    18721 aaaacgggcg gcattacgtc cattccccaa tacctgcaag acggatttga aatcaaatac
    18781 ttccccaaag gaacaggctt ccccgacctt gcccttcctg aaatgcccga cctgcccaag
    18841 gctgacgtta ccgccttttc cattgacgac gaatcgacca ccgaagtgga cgacgcttta
    18901 agcctgaccg acttggacaa cggcacgaag cgcgtcggca tccacattgc cgcgccgtcg
    18961 cttgccatta agcaaggcga caaaatggaa aaaaacatca tggaacgctt aagcacggtt
    19021 tattttcccg gcggcaaaat cacgatgttg cccgaaaact ggattgccgc gttcagcctt
    19081 gatgcaggta cataccgccc ttccatcagc atttatttcg atgtggacaa cgagttcaac
    19141 gtcggcgcgc cgacctgcaa aatcgaagcg gtcaacatcg ctgaaaacct gcgtatccaa
    19201 gccatcgaac cgcatttcaa cgccgaagcg ggcttggacg aagcgggcga aatgatgttc
    19261 gcccaccatc aagacctgat ttggttttat caattcgcca ccgccctgca aaaagcgcgc
    19321 ggcaaatacg aacccgaccg cgcgccgcaa tacgattaca gcatcgaatt ggataaagaa
    19381 gacaacgtat ccgtcgtccg ccgcgaacgc ggttcgccca tcgatacgct ggtcagcgag
    19441 atgatgattc ttgccaacag cacttgggcg caaatgctcc atgacaacga cctgcccggc
    19501 ctcttccgcg tccaacccac cggaaaagta cgcatgagca ccaaatccga gccgcatatc
    19561 ggcatgggcg tgcggcatta cggctggttt acctcgccgc tgcgccgcgc cgccgactac
    19621 atcaaccaaa agcagctgat cagcctgata gacgacactg ccgagccgct gtatcaaaac
    19681 agcgatgccg agcttttcgc cgcactgcgc gactttgatg ccgcctatac cgcctacgcc
    19741 gacttccaac gccaaatgga agcctactgg agcctggttt acctgcaaca gcaaggcacg
    19801 agcgagctga cggcgacgat tctgaaagaa gacctcgtcc gcatcgaagg tctgccgcta
    19861 gtaacacgcg cgaccggtat tccgtttgac gcgctgccca aatcgcaggc attgttcaaa
    19921 atcaccgaat tagatgccga gaagcagttt gtctcgctca actacatcaa ggcagtcgca
    19981 cccgcgggta caacggcagg caatgccgtc tgaagcccga tacggcaaac tccggcaaaa
    20041 acagaaacct gaatctcatc attcccacag tcgggaatcc ggttttttgg gtttccgttg
    20101 tttttccgtt tcaatgaact tccaagccgc cgttcccgat aaatacccgc aatctaaaat
    20161 cccgtcattc ccacgcagat gggaatctag gtttatccgc acagaaactg atatgccgtc
    20221 attcccgcga aagcggaaat ccagattctt cggtacagaa acttatcgga taaaacggtt
    20281 tctttagatt ctacgtccta gattcccgcc tgcgcgggaa tgacggcata ggggtttccg
    20341 ttttcccgat aaattaccac aacccaaaat cccgtcattc ccgtaaaagc gggaatccgg
    20401 ttcgttcggt ttcgtttttt ttgagtttcg ggcaacttct gaatcgtcat tcccacgcag
    20461 gtgggaatct aggtctgtcc gcacgggaac ttatgcgccg tcattcccgc gaaagcggga
    20521 atccagaccg ttcggcttca gttttttggt ttcgggcaac ttctgcatcg tcattcccac
    20581 acaggtggga atctaggtct gtccgcacgg gaacttatgc gccgtcattc ccgcgaaagc
    20641 gggaatccgg ttcgttcggt ttcgtttttt ttgagtttcg ggcaacttct gaatcgtcat
    20701 tcccacacag gtgggaatct aggtctgtcc gcacgggaac ttatgcgccg tcattcccgc
    20761 gaaagcggga atccagaccc ttcggcacag aaacttatcg gataaaacgg ttgctttaga
    20821 ttctacgtcc tagattcccg ctttcgcggg aatgacggca tagaggtttc tgttttcccg
    20881 ataaattcct gttacttttc gttgctagat tcccgcctac gcgggaatga cgatggaaag
    20941 attgttgttg cttcggataa atttctgcag ttttaaataa ccggattccc gcctgctcgg
    21001 gaatgacggc ataggttttt ttgtttttcc gataaattac cacaacccaa aatcccgtca
    21061 ttcccgcgac ggcgggaatc cagtccttta aactccagcc attcccgata aattcctgtt
    21121 acttttcgtt tctagattcc cgctttcgcg ggaatgatga atgacggtgt aaaagtaact
    21181 cgaaatccaa aaagccatac cgcccggatt tttgccgatc ggtatggctt tttgcttcaa
    21241 accgccttag cggatgtcga catacgcgcc ggaatgcagg tcacgcaaca ggttgacggt
    21301 tgcctgttcg gctttttgtt ggaagatgta ttgccgcacg gaattgcgga tacgttcctg
    21361 aggtgtgccg gcatcgcgca cttcgttcaa tttgatgata tgccagccga attgggtgcg
    21421 gacgggcgcg ccgacctgtc cgggtttgag cgcgtggacg gcttcttcaa aggcgggaac
    21481 catcacgccg tcggcaaacc agcccaaatc tccgccgttg cccgcgctcg cgtcttgcga
    21541 atattggcgc gctaggctgg aaaagtctgt gccgctgcgg gcctctccgt agattttgcg
    21601 gatggtgctt tccgcgccga cggcggcgtt ttcgctgtcg gccttaatca ggatgtgttg
    21661 ggcgcggtat tggcgcaacg gtgcgccttc gggcagggtg atgccttgtt tttgcgcctg
    21721 ctcgataaag cggtcgacct ctgcctcgcc gacccggctg ttttgcatca ctgcctgctg
    21781 gcggactttt tcggcaatga tgttgtcggc aagctcgcgg cgttgggcgg ggctgaggtt
    21841 tttgagggcg ggattttgtg cgacgacggc atcgatttcc gcttcgcttg cctgaatgtt
    21901 gcggcgtttg cccgcctgta cgatcaggga ttggttgaca agctgcatca gcacctgtcg
    21961 ggacagctcg gattcgctta tctgcgcgtc tttgggcagg ttggctttgg cttcggcaac
    22021 ggcttcggca agccggcggc gcgtgatgac ttcgttgtcg gcaacggcgg caatgccgtc
    22081 tgaaaagcgg ataccgccct gctgttgtgc gggtgcggct tctttttgcg ccgtggcggc
    22141 aactttggca actttggcaa ctttggcaac tttggcaact ttggcaactt tggcaacttt
    22201 ggcaactttg gcaactttgg caactttggc aactttggca gctttggcag ctttggcagc
    22261 tttggcagct ttggcagctt tggcagcttt ggcagctttg gcagctttgg cagctttggc
    22321 agctttggcg gatgcggttt ttgccttttg cggtgcggcg tggacatcgg ctgctgccag
    22381 caatgcggcg gcaatcatca gggctttgat tttcatcatt ctttccttat cgtttgaatc
    22441 ctgcctttga tggcgggttt ggggttgtcg gtggaatgcc gtcttgtgcg ggaacggtgc
    22501 tcctgccggg tttccgacgg tcagggccgt ttgttgcgtc cggcggaaag agagtgggcg
    22561 gggatgtagc cgggaacggc gacatccatc ctgtctgcgg ggtttctgcc gacactgctg
    22621 aggtctttca actgaagtga gaaaaagaca gcgtttttgt aggtgttttc gccggtaacg
    22681 tagcgttggg cgtacacgcc cgcgccccag cagccgcaac tgcttttgta ttccgcaccc
    22741 gccagcacct ctatcggttt tttggcttca aaaccgtagt tgtaacggac gacggccgac
    22801 aggttgcgcg tcagcggcca ttgtgcggac aggtcgagct ggctgagttt gtcgtaaaaa
    22861 taggaaccgt cggacttcag gtagattttt tcgttgcgcc cgtatttgta gcgggcgttc
    22921 agcactttgc cctgtgcggg acggtagctt gcaccgacgg cgtagttctc ggcgcgtttg
    22981 tcgttttggt tgtagtggat gctgctgtcg aggatgaagc ggctgccgat gctgccggag
    23041 gcaaatgcca cccagtcgga acggttgcgc ggttttttgc cgacgctgcc gtcaagcatc
    23101 accgcatcat ccttgaaata gaatttctga ccgatgccgg cgcggaaacg ctcttccccc
    23161 gtcgcgccgt ccaaaatacg gctttgcacg gcggcggaaa ggctgtttgc ggtgttaatc
    23221 ctgtcgttgc cgtaatagag gttttcgcga aagagctgcc cgtagccgaa gctgctttcc
    23281 gacgaatcga aattgggcag gtcgttttgg gatttggcag gaatatagtt gtagaacagg
    23341 cgcggctcga gggtttgcag gactccgccg ccgaacatcc gcgtattccg ctcaaaagtt
    23401 gcgccgctgt cgatgttgac aatgggcaga gtgcggctga cgcgtcgggc ttcttggctg
    23461 ccgaagcggt tgaggctgta ataggtggcg tgcagtccga gtttgggacg gacgtaaccc
    23521 cagctgttgc tgaaatccca tttgatgtcg ggatagacga cgaggcggct gccgtcttgg
    23581 cggctgtcgt ggctgaagcg ggtaaattgt gcggacacgc cgatttgcgc cctgccggtg
    23641 tttttgcgcc aatcggcgga aaggcgcggc atcagggcat acggtttgtc tttgtagccg
    23701 ctttggtttg ccagcgtctg gtatttcaga accgaaaggc cggcattcag gctgccgccc
    23761 gccgccctgc cgccataatc cagccataca cggcggttga ggttgacgtt gccggcgatt
    23821 tctttgttgc cgtaaaagtc gcggtagtag ccgctgtcgg agacttggtt gaaatcgaca
    23881 cccgcctgaa gcgtgtcgga aatgtcgtgc cggtgctgcc atttcgcctg atagcggtta
    23941 ttcctgccgc tttttttgtc gtgcggcagc caggtcaggt cggactggcc ggcataatcc
    24001 ggccgcaggt agcgtacctg cccgtcaaag accgcgccgc gttcgccgat cacgccgggc
    24061 gcgaacgtgg catcgagatt gggggcaagg ttgaaataat agggaacgga aagggaaacg
    24121 ccgtccgaac cggcggacag tgagggaaca agcaggccgc ttttgcggtt gccgtcaagc
    24181 gggaagtccg cccaaggcgt atagaaaagg ggaacgccgc cgaacacgaa ggcggcgtgt
//
LOCUS       DAWHXS010000017        24142 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWHXS010000017 DAWHXS010000000
VERSION     DAWHXS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.017"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            358..981
                     /locus_tag="WIE22_000537"
     CDS             358..981
                     /locus_tag="WIE22_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ4126678.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1092..2474
                     /locus_tag="WIE22_000538"
     CDS             1092..2474
                     /locus_tag="WIE22_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4126679.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQDGLQCALSQLEGVEDIGFSFDRQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2478..3002
                     /locus_tag="WIE22_000539"
     CDS             2478..3002
                     /locus_tag="WIE22_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ4126680.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            3527..4450
                     /locus_tag="WIE22_000540"
     CDS             3527..4450
                     /locus_tag="WIE22_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4126681.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(4515..5903)
                     /gene="fumC"
                     /locus_tag="WIE22_000541"
     CDS             complement(4515..5903)
                     /gene="fumC"
                     /locus_tag="WIE22_000541"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ4126682.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDVCNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(6044..8023)
                     /gene="tkt"
                     /locus_tag="WIE22_000542"
     CDS             complement(6044..8023)
                     /gene="tkt"
                     /locus_tag="WIE22_000542"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ4126683.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            8550..10046
                     /gene="ccoG"
                     /locus_tag="WIE22_000543"
     CDS             8550..10046
                     /gene="ccoG"
                     /locus_tag="WIE22_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ4126684.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            10049..10597
                     /locus_tag="WIE22_000544"
     CDS             10049..10597
                     /locus_tag="WIE22_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ4126685.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(10938..12113)
                     /locus_tag="WIE22_000545"
     CDS             complement(10938..12113)
                     /locus_tag="WIE22_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ4126686.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(12672..12746)
                     /locus_tag="WIE22_000546"
     tRNA            complement(12672..12746)
                     /locus_tag="WIE22_000546"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12711..12713),aa:Glu,seq:ttc)
     gene            complement(12764..12840)
                     /locus_tag="WIE22_000547"
     tRNA            complement(12764..12840)
                     /locus_tag="WIE22_000547"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12804..12806),aa:Arg,seq:acg)
     gene            12931..14343
                     /locus_tag="WIE22_000548"
     CDS             12931..14343
                     /locus_tag="WIE22_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ4126687.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGRLHAHKVKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGILNAKTADLKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            complement(14548..15327)
                     /locus_tag="WIE22_000549"
     CDS             complement(14548..15327)
                     /locus_tag="WIE22_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ4126688.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            15601..17460
                     /locus_tag="WIE22_000550"
     CDS             15601..17460
                     /locus_tag="WIE22_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ4126689.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGIKNPIGRRTMKKATVPFMPKNAGMPRK"
     gene            17496..18689
                     /locus_tag="WIE22_000551"
     CDS             17496..18689
                     /locus_tag="WIE22_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4126690.1"
                     /translation="MYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGSPVFTGFSG
                     TVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYET
                     SQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGS
                     YTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVD
                     FGYVPGAGGKQGSFQTVAKPIGKVVSRGAEAELSGELNEDWKVFAGYTYNKSRYKNAA
                     EVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI
                     RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMK
                     LDWQF"
     gene            18833..19891
                     /gene="dinB"
                     /locus_tag="WIE22_000552"
     CDS             18833..19891
                     /gene="dinB"
                     /locus_tag="WIE22_000552"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ4126691.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYDSEVAKEIRAAIFAETGLTASAGIASNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMQTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCASLLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(19959..21974)
                     /gene="rep"
                     /locus_tag="WIE22_000553"
     CDS             complement(19959..21974)
                     /gene="rep"
                     /locus_tag="WIE22_000553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ4126692.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLHYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(21994..22758)
                     /locus_tag="WIE22_000554"
     CDS             complement(21994..22758)
                     /locus_tag="WIE22_000554"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ4126693.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRCAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLESGAD"
     gene            23012..24058
                     /gene="recA"
                     /locus_tag="WIE22_000555"
     CDS             23012..24058
                     /gene="recA"
                     /locus_tag="WIE22_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ4126694.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
BASE COUNT         5442 a         5924 c         6880 g         5896 t
ORIGIN      
        1 ctcaagatat agtggattaa atttaaatca gtacggcaag gcgaggcaac gctgtactgg
       61 tttaaattta atctactata tttccgcctg ctgccgcccg aaaagcgtga tgcgctgatg
      121 gtgctgctgc acaatccgga cgacggatag ccgttgaaaa cgggaaatgc ccgggcggtg
      181 tcgcctgtgc ctgtttggac aagcgttctt gagggcggta gaattgaggt ttgcccgaaa
      241 agggcagggc gatatgccgc gcttggattt tgcgcgcggt ttttcgcgtt ggaagcaatc
      301 attgtctgtt tgaatattta atgccgtctg aaacgtccgg acgcgccccc gttccctatg
      361 aaaaaaccga ccgataccct acccgttaat ctgcaacgcc gccgcctgtt gtgtgccgcc
      421 ggtgcgttgt tgctcagtcc tctggcgcac gccggcgcgc aacgtgagga aacgcttgcc
      481 gacgatgtgg cttccgtgat gaggagttct gtcggcagcg tcaatccgcc gaggctggtg
      541 tttgacaatc cgaaagaggg cgagcgttgg ttgtctgcca tgtcggcacg tttggcaagg
      601 ttcgtccccg aggaggagga gcggcgcagg ctgctggtca atatccagta cgaaagcagc
      661 cgggccggtt tggatacgca gattgtgttg gggctgattg aggtggaaag cgcgttccgc
      721 cagtatgcaa tcagcggtgt cggcgcgcgc ggcctgatgc aggttatgcc gttttggaaa
      781 aactacatcg gcaaaccggc gcacaacctg ttcgacatcc gcaccaacct gcgttacggc
      841 tgtaccatcc tgcgccatta ccggaatctt gaaaaaggca acatcgtccg cgcgcttgcc
      901 cgctttaacg gcagcttggg cagcaataaa tatccgaacg ccgttttggg cgcgtggcgc
      961 aaccgctggc agtggcgttg attttgaacc cgcgccgcaa ccgaaatacg gcgaatcctg
     1021 tataatccga aaatctgttc actggaagtt cagacggcat tgcaactgtt gatgccgtct
     1081 gaaaaaatat gatggcaaga gataaccgca tccaaatgtt tccgcacgaa tggcgcgcca
     1141 gtacgacgct ttccggcgtg tacgcgctgc gtatgctggg tatgttcctc gtgctgcccg
     1201 tattggcggt gtatgccgcc tcgctgcccg gcgcggaagg caacaaaacg ctggtcgggc
     1261 tggcaatggg catttacggg ctgacacagg ctctgctgca actgcctttg ggcatcgctt
     1321 ccgacaagtt cgggcgcaag aaaaccattt atgtcgggct gattgtgttt gcagcgggca
     1381 gcttccttgc cgccgccgcc gatacgctgc ccatgctggt cgccgcacgc gccatacagg
     1441 gtgcgggagc ggtcagtgcg gcggttaccg cgctgctggc ggatttgacg cgcgacggcg
     1501 tacgtacccg cgcgatggcg atgatcggtt tgagtatcgg tctgacgttt tcggtcagcc
     1561 tcgtcgttgc ccctgtgatt gccgacgcgg tgggcgtacg cggcttgttt ctgctgaccg
     1621 gtattttgac cgttgccagt atcggcgtgg tggcgtggat gactcccgac ccggaggttt
     1681 ccaagctgca cgaagatacg caggcgcagc cttcgcgcat aggcgaagtt ttgaaaaacc
     1741 gtaggctgct gacgctcgat ttcggcattt tcgccctgca cgccgcgcaa atggcattgt
     1801 ttaccgcgct gcctttcgcg atgacccagc tcggtttgga aaaaatccag cactggaaag
     1861 tctatctgcc ctcgaccatt acgggattgg tggtaatggt tccgctgatt atcgtcggcg
     1921 agacgcgcaa caagcttaag caggtttttg ttttgggtat cgtctgtatt gcggcggcgc
     1981 agctcggttt gctgtccggt atgcgctcgg taggcttgat taccgcttat ttggttgttt
     2041 actttatcgg ttttaatgtg ttggaagcga gcctgccgtc tatggtttcc aaaatcgcgc
     2101 cgtccgacct gaagggtacg gcgatgggcg tgtacaacac gatgcagtcg ctcggactgt
     2161 ttgccggcgg cgcggcaggc ggtttgctgt ttcaaaaata cggcttttcc ggcgtgtttg
     2221 ccttttgcag tatattgatg ctgctgtggc tggtaattgc cgttttatcg cccgcgccca
     2281 agcccgtcaa aaacctcagt taccctgtcg gcggcgtgtg gcagggcaat caggacggct
     2341 tacaatgcgc cttgtcgcaa ctcgaaggcg tggaagacat cggtttcagt ttcgacaggc
     2401 agaccgtcta tctcaaagtg ttgcagaagg gtttcgatca ggctgccgct gaaaaaatca
     2461 tcacaggagt ttaaaaaatg tcattgaaca aagtcatcct tatcggccgt ctcggacgag
     2521 atcctgaagt acgctatatg cccaacggcg aagccgtctg caatttcagc gtcgccacca
     2581 gcgaaacttg gaacgaccgc aacggtcagc gcgtagagcg caccgagtgg cacaacatca
     2641 cgatgtaccg caaactggcg gaaattgccg ggcaatacct caaaaaaggc gggctggttt
     2701 atctggaagg cagaatccaa agccgcaaat accaaggcaa agacggcatc gaacgcactg
     2761 cttacgatat tgtcgccaac gaaatgaaaa tgttgggcgg gcgcaatgaa aacagcggcg
     2821 gtgcgcctta cgaggaaggt tacggtcaga gtcaggaggc ttaccaacgc cccgcgcagc
     2881 aaagccggca gcccgcctcc gacgcgccgt cccatcccca agaagcacca gccgcgccgc
     2941 gccgccaacc cgtgcctgcc gccgccccgg tcgaggacat tgacgacgac atcccgttct
     3001 gaattttacg gcagcacatc ctcgcggagg gaaatcataa aggacggaga aaccttaaac
     3061 cttacggggc agggtttctc ttttttgcat ttgtgccggc ttgttgctgc cccctgcgtt
     3121 gggcggaacg ttgcgattgc gccgttctgg cgttttgagg cagtgatgcc tgccgcaggc
     3181 ccacgctgtt ttcagacggt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     3241 cagacagtac aaatagtacg gaaccgattc acttggtgct tgagcacctt agagaatcgt
     3301 tctctttgag ctaaggcgaa gcaacgctgt actggttttt gttaatccac tatattttaa
     3361 aattcaggcg gtgttttcaa atagcgcgaa gcaatagccg agcttcagcc ggtttgtttg
     3421 aaatcaaagg gttggaatta aacgccaaac gcttgaacca ttattgcccg atgcggcaaa
     3481 ccggcatcgt ccggcaatac ggttgtttaa aaataggaaa atcaggatgg aaaaccaaag
     3541 gccgctccta ggcttcgcgt tggcactttt ggcggcgatg acgtggggaa cgctgccgat
     3601 tgccgtgcgg caggtattga agtttgtcga tgcgccgacg ctggtgtggg tgcgttttac
     3661 cgtggcggcg gcggtattgt ttgttttgct ggcactgggc gggcggctgc cgaagcggcg
     3721 ggatttttct tggtgctcat tcaggctgct gctgctcggc gtggcgggca tttcggcaaa
     3781 ctttgtgctg attgcccaag ggctgcatta tatttcgccg accacgacgc aggttttgtg
     3841 gcagatttcg ccgtttacga tgattgttgt cggtgtgttg gtgtttaaag accggatgac
     3901 tgccgctcag aaaatcggct tggttttgct gcttgccggt ttgcttatgt tttttaacga
     3961 taaattcggc gagttgtcgg gtttgggcgc gtatgcgaag ggcgtgttgc tgtgtgcggc
     4021 aggcagtatg gcatgggtgt gttatgccgt ggcgcaaaag ctgctgtcgg cgcaattcgg
     4081 gccgcaacag attctgctgt tgatttatgc ggcaagtgcc gccgtgttcc tgccgtttgc
     4141 cgaaccggca cacatcggaa gtttggacgg tacgttggcg tgggtttgtt ttgcgtattg
     4201 ctgcttgaat acgttaatcg gttacggctc gttcggcgag gcgttgaaac attgggaggc
     4261 ttccaaagtc agcgcggtaa caaccttgct ccccgtgttt accgtaatat tttctttgct
     4321 cgggcattat gtgatgcctg atacttttgc cgcgccggat atgaacggtt tgggttatgt
     4381 tggcgcactg gtcgtggtcg ggggtgcggt tacggcggcg gtgggggaca ggctgttcaa
     4441 acgccgctag ttcgcaggca acggaaaatg ccgtctgaac gaggtttcag acggcatttt
     4501 atttgaggga aagattagcg cggatgaacc atatcggcag gaacgaccag ttcgtcaaat
     4561 tcttcgcccg tcagcaagcc cagctcaacg gcggtttcgc gcaacgattt gttgtttttg
     4621 taggcggttt tggcgacttt ggcggcgttt tcgtaaccga ttttgcggtt caacgcggta
     4681 acgagcatca gggaatggtg caggaaatag tcgatttttt ccggtacggg ttcgatgccg
     4741 acggcgcagt gttcgttgaa gctgttgcac acgtcgccca agaggcggat ggattgcaag
     4801 aggttgtagg cgataacggg catatagacg ttcagctcga aattgcccga cgcgcccgcc
     4861 ataccgatgg taacgtcgtt gccgaacact tggcagcaca ccatcgtcat tgcttcgcat
     4921 tgggtcgggt tgactttgcc cggcatgatg gacgaacccg gctcgttttc ggggattttg
     4981 atttcgccca aaccgcaacg cgggccgctt gccagccagc ggatgtcgtt ggcgatttta
     5041 ttcaggcttg ccgccagcgt tttcaatgcg cccgaagcgg caacggcggc atcgcgtccg
     5101 cctaaggctt caaatttgtt cggcgcgctg acaaacggca agccagacag ttcggcgagt
     5161 ttagcagcag ctttttcggc gtattcggga tggctgttca aacccgtgcc gaccgccgta
     5221 ccgcccaaag caagttcata caagtcttta agcgcatcgt tcagacggcc taaaccgtga
     5281 tcaagctggg aaacgtagcc ggaaaattcc tgtcccaaag tcagcggcgt cgcgtcttgc
     5341 aagtgggtgc ggccgatttt gacgataggg gcgaaagctt gggctttttt gtccaacgtg
     5401 tcgcgcaggg cttttacggc ggggatgagg tggcggttga tttcaatcgc ggcggcaacg
     5461 tggatagcgg tcgggaatgc gtcgttggtc gattgcgcgt ggttcacatg gtcgttggga
     5521 tggacgggct gataagccgc caaacccgta ccggcgattt cgttggcgcg gtttgccagc
     5581 acttcgttca tgttcatatt ggactgcgtg ccggagccgg tctgccaaac cactaaagga
     5641 aactgtccgt caagcctgcc gttcaacaca tcatccgccg cctgcgtaat caaatccgcc
     5701 tgttccggct taatcctgcc gagggaaaca ttggtcgcgg cggcggcttt tttcaccagc
     5761 gccaaagcat aaatcagcgg ctgcggcagg gtttcgccgc cgattttgaa attgttgcgg
     5821 ctgcgctggg tctgcgcgcc ccaataggct tcggacggga cttcgacatt gcccatggtg
     5881 tcatgttcgg tacgggtgct catgcgtttc tccttttgaa atgtgaataa gagtgattcg
     5941 caaatattat aatggagatt ggcggaatga ggaagcatca aggcgggagg gcaaaaaaat
     6001 gccgtctgaa aagcgttcag acggcatttg tttaggtgtg gggttacagc acggatttca
     6061 ccgtatcaac cacattgtcc acggtaaagc cgaatgcttt gaagagtaaa tcggcagggg
     6121 cggattcgcc gaagcggttg atgccgacga ctgcgccgtt cagtccgaca tatttgtacc
     6181 agccgtcggc gtggccggct tctacggcga tgcgcggcag gctttcgggc aggacggcgg
     6241 cttgataggc ggcgtcttgg cggtcgaata cgttggtgga cggcatggaa acgacgcgca
     6301 cggcgatgtt ttgcgcggcg agggcttttt gcgcttccaa agccagctcg acttctgagc
     6361 cggtggcaat gatgacggct tgggcgttgc cttgggcttc gctgatgatg tagccgccgc
     6421 gtttgatgtc gtccagttgt tgttcgctgc gcgcttggaa tttcaggttt tgacggctga
     6481 aaatcaggca ggacgggtga tcggcggctt tgacggcttc tgcccaagcg accaaggatt
     6541 cggcggtgtc gcacggccgc catacgtcca tattcggaat caggcgcagg gtggcggttt
     6601 gctcgatggg ttggtgggtc ggaccgtctt cgcccaaacc gatggaatcg tgggtgaaca
     6661 caaatacagg gttgattttc atcagtgccg ccatacgcag ggcgttgcgc tcgtattcgc
     6721 tgaacatcag gaaggtcgca ccgaatggtt tgatgccgcc gtgcagggca agaccgttca
     6781 taatcgcacc cataccgaac tcgcgcacgc cgtagtggat gtagttgccg cctttgtcgc
     6841 gggtaacgga gacgctgttt gaccagtcgg tcaggttgga cggggtcagg tcggccgaac
     6901 cgcccaccaa ttcaggcaac tcttttgcca agatttcgat gctgttttgg ctggctttgc
     6961 gggtagcgat ggtttcggct ttggcgcaca cttctttcaa tgccgtctga acgtattcat
     7021 cgaaattgtc cggcagcttt ttatccatac ggcgcacaaa ttctgcggct tcggcaggat
     7081 atttggcttg atattgcgcg aacagttcgt tccagtcggc ttccagtttc gcgccttgtt
     7141 ctttggcatt ccacgcatcg taaatttctt gcgggatttc aaaggcgggg taagtccagc
     7201 ccaaatgttt gcgcgtggct tcgatttcgt ccgcgcccaa aggtgcgccg tgggttttgt
     7261 ggctgccttc tttgttggca ctgcctttgc cgattaaggt tttgcagcag atgatggacg
     7321 gtttgccggt ttcggcacgt gcggcttcga tggcggcttg aatggcggcg gtgtcatgac
     7381 cgtttacgtt aggaacgacg tgccagccgt agctttcaaa gcgttgcggg atgttttcgg
     7441 taaaccagcc gtccacttta ccatcaatgg aaatattgtt atcgtcatac aaaacaatca
     7501 gtttgcccaa gcctaaagtg ccggcgagcg aacaggcttc gtgcgatacg ccttccatca
     7561 gacagccgtc gcccatgaag acgtaggtgt aatgatcgac gatgttcaaa ccgtctttat
     7621 taaattcggc ggcaaggatt ttttctgcca atgccatacc caccgcgttg gcaatccctt
     7681 gccccaacgg gccggtcgtg gtttccacgc cgtcggtgta gccgtattcg gggtggccgg
     7741 gggttttgct gtgcagctgg cggaagtttt tcaagtcttc aatgctcaga ttgtagccgg
     7801 tcaggtgcag caggctgtac aacagcatag acgcgtggcc gttggagagg acgaagcggt
     7861 cgcggttgta gaatttgggg ttggcggggt tgtgattgag gaatttcgtc cacaatgttt
     7921 ccgccatttc cgccataccc ataggcgcgc cggggtggcc ggaattggct ttttgaacgg
     7981 catcggccga gaggaagcgg attgcgtttg ccagttgaga cattttgtat tttccttgct
     8041 ggtgtttcag ataagtggat aatcggaaag cgttgattat cgcccgattc gcttatgctt
     8101 tcaagaaaag ggcggacgcg tgggaaggcg gcggcagggg acaggcgggg aaggattttc
     8161 gatttgcggg cgaagcctgc cattattcct tttgaaataa aaagttatag attgtgtgcc
     8221 ggattgtcga tagcgtttgt ttatgagctt gcgccgtcgg ttctgccgat atgggggtgt
     8281 cggttttttt agtctttttt ttaaccgtat tcggattttg ttcgggcggt aaggtaaaat
     8341 ccaggcgttt tgatgcggat gggaaatgta tccgccccat acatccggac ggcgcataaa
     8401 gttgtacaat agcggaaata tattttggga aaaacggatt ttctcaaaac ttgaaacaca
     8461 acgccttaaa aaacaagaaa aatgacgtct gaaaagcaaa cggcggcggt aacgggcaaa
     8521 ccatcaatca aaaatctaag gaatgcagaa tgaccacgga aaaccaagcc ggcagtccgg
     8581 catccggaat cggcacgtcc gaacaaacaa aagccgcgcc gaaagcaaaa aaaacattcg
     8641 atccgcgagc cagcgtgatt caaatccatc ccgaaggcga acgcatccat cccaaaaagg
     8701 cggaaggacg gttcgccaaa ctgcgtatcg ccgccgtatt ggcgacgcag tttgtgtttt
     8761 acgtcattcc gtggttcaac tggagcggca ggcaggccgt cgttttcaat atccccgaac
     8821 ggcatttctt cattttcgga ttgtcgttgg gggtgggcga tttgatttac cttgccttgc
     8881 tgctgatgat ttgcgccttc gggctgtttt ggtggacgac gattgcaggg cgactgtggt
     8941 gcggctattc ctgcccgcaa acggtttaca ccgaaattat gctgtggatt gacaacctgg
     9001 tcgaaggcga tagaaacaaa cggctgaaac tggaaaaatc gccgtggaat ttcactaaaa
     9061 tccgcatcaa agccaccaaa tacctgctga ttttccttgt ctgcgcgtgg acgggcatca
     9121 cgtttgcagg ctggtttgtc cctatccgcc agttcgttcc cgatttattc actggagcag
     9181 caggtggcgg cgcgatgttt gccgcagcgt tttatggctt tatgaccttc ttcttcgccc
     9241 acattatgcg tgaaaaagta tgcctgcata tgtgtccgta tgcacgtttc caaagcgcga
     9301 tgttcgacaa ggacacgctg attgtttctt atgacgcgga acgcggcgaa ccgcgcggcg
     9361 cgcgcaagaa aacggtcaat aaggaagagg cgggtttggg cgactgcatc aactgtgcga
     9421 tgtgcgtcca agtctgcccc gtcggcatcg acatccgcaa cggtctgcaa taccaatgta
     9481 tcggctgcgc cgcctgtatc gacgcgtgcg atgagattat ggacaaaatg ggctatccgc
     9541 gcggattaat ccgttatacg accgaaagcg cgctggaaca cgaatattct gaaaaagaca
     9601 ttaaaaaacg gctgctcaga ccgcgcgtgg caggttacgg cgcggtgttg gcgttagtta
     9661 tcattgcctt cctggccggt ttgtccacgc gcaaaatggt cgaggtcgat attttgaaag
     9721 accgtggcgt actggtgcgc gaaaacgcca aaggctggct ggaaaacgca tacagcctgc
     9781 gtatcatcaa caaaagtgaa aaagaacagc tcattaccgc aagtgtcaaa ggctttgacg
     9841 aaatcgccct gaccgggctg cccgaaggcg gtatcaaggt tgccccgcgc gaaacggtaa
     9901 cccttcccgt ccaagtgtcc accattccgg aatacgcgga caaaggcagc caccctatcg
     9961 aatttacctt ccaataccgc gaaagcggcg cgcccgacgg caagccggtc gtcttggaag
    10021 aagatgcaac ctttatcgga gaataaccgt gtctcaaaac actccaatca aaccttggta
    10081 caaacacgtc tggccgtgga tcttgatggc ggggccgatt tttgtcgtca tcgccagcgt
    10141 cgctatgttt tttgtcgcgc agcagcacgc gacagatttg gttacggacg attattataa
    10201 agacggcaaa catatcgaca tccagcttca tcgggatgaa gaagccgtca gacggcatat
    10261 cggggtgcag gttctcattt cccccgatat gaatgcggca aaagtgtttg tcggcggcga
    10321 gtttgacggc aaacagcctt tgaacctgct gctgatgcac ccgacccgca aggcggacga
    10381 tcaaaccgtc gccctcaagc ccgtcggcag cgcgcagaac ggcagggcgg aatatgaggc
    10441 ggtgttcaaa accctttcgc cgaccaacca ctggtatgtg cgcgtggagg acgcggcagg
    10501 cgtgtggcgc gtcgagaaca aatggattac cagccaaggc aatgcggtgg atctgacccc
    10561 gatggataaa cttttcaata atgccggaag caaataaaaa agccgtgttt tcattgcatt
    10621 ggtttgccag atgcaatgtc gggcaggcat tgtttgatta ttcggcgtga tggtctgatt
    10681 tatcggacga aaataaatgt atcttatccc gtttattttt taacaccatt ccgaaatgca
    10741 gcctgaaaaa tgccgtctga agggcttcag acggcatttg tgttgtatgc ccgacacaat
    10801 gcggaaggcg ttttgtttac ataaaaacgt ttttatttct ctccgttttt tcgacactat
    10861 tccgaaacgc agcctgaaaa atgccgtctg aaaggcttca gacggcattt tgtttgcaaa
    10921 tcaaatccta cctgatgtca gcgtccgagc gttgcaacag gttcgctccc cgtcagcaga
    10981 tattgtatcg tctcgcggac ggtgcggatg gcattgccga agggcagcgg gtctttacct
    11041 ctgaagaaca ggcctttatc tacttctcca cggaatgcgg cggcaagctg gatatcaatg
    11101 cagaattgtc cggctttgga aagcccgtcg cgcagaccgc aactggtcag gcagtttaaa
    11161 ccttgggtac agcggcgcgg gtcagctttg gcgtttgcct gaagtttgct ttcacgcttg
    11221 atgtagctgt ctaggaattt ggtgcggaca ccgcgcgccg gcaaaccggc aacagacata
    11281 aattcaacta ctttttcagt ttccgcaccg gcgagcgttt ttttgaagtt aaggtgtgca
    11341 tctccttctt cggtaacggc aaaagccgta ccgatttgaa cggcggatgc tccccagttc
    11401 tttagggcgg ttttgacttt ttcaaaattt gccatgcctc ccgcaagaat aagcgggatt
    11461 ttttcgcttt ccagccctaa acttttgaaa acttcaaacg tttcctcaat cacgcgtttg
    11521 aagtcgaact tggcatcgtt tacgccttca acggttgatg cacccaaatg tccggccgcg
    11581 tgggcaggat gttcgactac aatcgcatcg ggcaatatgc cttttttcat ccaacgtttc
    11641 aagacgatat taataccgcg cgattcggac agaatcggca gcagcgcgac atctttatga
    11701 tagccctcgg tcatttccgg caggtctaaa ggcaggccgg cacccattac aaccgcatcc
    11761 gcccctgatt cgcaagcctg gcggacatac gcggcgtggt ctttgaccgc cttcatcacg
    11821 ttgaccgcaa tcagtccttt tccctctgaa gcgcttttgg ctttttggat ttccctgtct
    11881 aatgcggtac agttcaaaga tgtatatttc tcttcactcg gattgatttg tgattcggcg
    11941 agtaggtctt cgtgaaggtg gcgcaaatcc acactggcaa tcgttccgat accgttttca
    12001 cgtgccaccg cgctggataa acccgatgcg gaaacaccga cccccatacc gccttgcacg
    12061 atgggggtaa gggattttcc acgaataacc aaagggtcaa aaatattttg catcagtttc
    12121 tccgagtata cgaatcagtc aggctcatga aaaatggttc taactgtttt agaactattg
    12181 ctcaaatttg gtattatact ctaaatatcg cggcggtaaa caagaaagca gccggagctt
    12241 cgggcatatt ctgttacgcc tgtttcaatc gggagagaaa atattatagt gaattaacaa
    12301 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac
    12361 gccgtactgg tttaaagtta atccactata aatttgatga tggcataaaa aagccctagt
    12421 cagttgctgt caaaggggat tgttaagaaa agtaatgcca cgttgatggg gtgcaatata
    12481 tcaggcgttt caatcgggta aaatgttgga cggacaccgc atctggttat gggatatcgg
    12541 tttgcacggc aaaggtttga atatgattcg attgtgtctg agattctgat gaagggggta
    12601 ggtaaagata tggtaacgag ttgccgagac aataaaaaat ccccgaggga taatctcggg
    12661 gatttggaat ttggcacgcc cacggggaat cgaaccccgg ttaccgccgt gaaagggcga
    12721 tgtcctaacc gctagacgat gggcgcatat tgtattttct tactggcgca cccggagcga
    12781 ttcgaacgcc cgaccctctg gttcgtagcc agatactcta tccaactgag ctacgggtgc
    12841 gctgcaagaa agatttgaat tttagggtaa tcatcggatt ctgtcaattg ttttgttgga
    12901 tgaaatgcct taaaagtgta aaatatcggt ttgttgtttg aatttaaaga gaataaaatg
    12961 attgttgctt cccgctggcc cttgctggaa aaggtatttt tacggtttgg aatgccggtt
    13021 gccgtggtcg atttggaatc gacgggcggc aatctgtatg aagacagggt aaccgaagtg
    13081 gctttggtca agtttgagca gggaagggtg gtgaggcatg agtggttggt taatcctcaa
    13141 aaaccgattc cgcagtttgt ggcggggctg acggggattt cagacggcat ggttgccgat
    13201 gcgcctgttt ttgcagagat tgccggcgag ttgttttcgg tattgaaggg ttgtgtgctg
    13261 gttgcacata acagccgttt cgactatacg tttttaaagc atgagtttca tcgtgcgggt
    13321 atcggatttt catcgcctgc tttgtgcagt gtgcagctgt cccggtgtct gtatccgcaa
    13381 ttttacaagc acagcctgga cagtatcatc gaaaggttgg ggattgttgt ggaagacagg
    13441 catcgtgcga tggcggatgt gtcggcgttg tgtgattatt tggaatacag cctgtcggaa
    13501 cacggggtcg aggagtggat caggcagtgt ttccgtttga tgaatccgaa accgttgcct
    13561 gccgcgctgc ccgaacggtt gagggaacag ttgtacggtt tgcctgacgg tatgggggtg
    13621 ctggcctgtt tcgacggcgg agggaaagta aattacatcg gtacgtttga acgggtatat
    13681 agcgaggttt cggctttatt ggactccgga aaagccccgt ttgattggtg caatacggag
    13741 gaagtccgtt tttttcccgc attgggcagg ctgcatgccc ataaggttaa agcggaattg
    13801 gtcaggcatt atggggtgga ttgttacgta tctgccaaaa atctgcttaa aacatttacg
    13861 accgtcaggt ttgaaaaagg ttcagacggc atattgaatg cgaaaacagc agatttaaaa
    13921 aacggtgtga cggataatcc gcctaccgga ttatttgcca ataaaaaggc ggcgagacgg
    13981 gctttgtcgt cgtgggcgga aacatacgga ttgtgtcctg ccgcagccgg tatcctgccg
    14041 gacggttatg cagaggatga gccttgtccc gtatatgttt caggccgatg cgataaggcg
    14101 tgcggccgtt ctgacgaaca ggttttggcg tttgcacaca agttgcctgt tttggattgg
    14161 ggaaaaatgc atgaggtgga aattaccgaa accgatcctt taacggggga aaaagttgtt
    14221 ctgcatggaa tgggcggcgc attggaaatg gatgacgggc tttggtattt cgataaagat
    14281 ttaccggatg cgtttaaggc gaagtttaaa acggacagga aaaatattaa ggaaatcggt
    14341 taaatcgata tcggttggtg aaagccggta agtatgtaaa cagccgttcg tattgattgc
    14401 ctaatcggtt tgggttggaa tatgttgccg tttgtatgta tccgaaaatg ccgtctgaag
    14461 gcttcagacg gcatttttgc cgacgcggtt ttcgtgccgt gtcggtttga ccgtccatcc
    14521 gaaggcatcg gacaagcagg gtggttttta gaagccgttt tccatcatga tttgaccggg
    14581 aatgcggttg cggtgcatgg cgtaacccat gtcaagcagc gtttggaaag tatctttgac
    14641 catttccggg ttgccgcaaa tcataaaccg tgtggattcc ggggtgaact tggtatgcag
    14701 cgcctgttcg atgctgctgt tttttaagag ttccggaatg cgttttccgc ttaagcccga
    14761 ggggttggcg gcacgggtgg taacagggac gaaatggaac gagtgtctgt attcgcctac
    14821 caggggatgt tcggacaatg cggcgagccg gtcgttgaaa atcaattctt cgggaaaaga
    14881 tacggaatgt atcaggttga cggtatcgaa acgctgtcgg atttcaggtt gttcgagaat
    14941 ggaaaggaag ggggcgatgc cggagccggt gcagagcatc accaaatcct tgccgtcggg
    15001 gaagcgttcg ggcaggagga agccggtggc atttttatcg agcaggatgg tgtcgccctg
    15061 ttgcattttg gcgaaacggg ccgacatggg gccgtcttgg atgagtacgg caaaatattc
    15121 gagcgtgtct gcatattctg cggaaacgat ggagtaggca cgccaaataa agccttcccc
    15181 ttcgtagaaa ccgagtcggg agaactgtcc ggctttaaaa cggtaggatt cgggacggct
    15241 gatggcgaaa gtgatgagtt tcggcgtgtg gtgtttgacc cacaaaatct tttcttcagt
    15301 gaattttgct tcgggcgagg ctgccatgtt gttgtccttg agtgtgtttg tcgggattat
    15361 agccaatttg ggtgtttttt tccgcattga cgatctgtat ttaaattcgg atatttttca
    15421 atacaatatt atcgtttttt gaaataaacg gtaaggagca gtttgttttt tgtcaaaatg
    15481 ggaatacaat cccatctata aatttaattg agaattactt tttttcaatt aatataaaaa
    15541 atcaaaacag ttgtgagggc ctttggacga tttgcacact tgcatattac agaggtctct
    15601 atgaatcaaa atcatttttc acttaaaatt ctgaccgtta tgctgttatc ggcttacggt
    15661 ggttcttttg cagacggtgt tgtgcctgtt tcagacggca ataccgtcag tctggatacg
    15721 gtcaatgtac gcggctctca tgctctgtcg ggcaagaccg agaagacccg ttcttatacg
    15781 atagatcgga tgtccaccgc cacaggtatg aggattgcgg gcaaggatac gccgcagtcg
    15841 gtcagcgtca tcacgcgcag ccgccttgac gataaggcgg tgcatacgct tgaagaggca
    15901 atgaaaaaca cgacgggtgt caacgttgtg cgcgattcag gcttgcagac gcggtttttg
    15961 tcacgcggtt tctatattga tcagattggt gaagacggta ttaccgtcaa tgttgcaggc
    16021 cgttcgggat atacggcgaa aatcgacgtg tctccgagta ccgatttggc ggtttatgac
    16081 catattgaag ttgtacgggg tgcaacgggg ttgacccaat ccaattcaga gccgggtgga
    16141 accgtcaatt tgatccgtaa gcgacctacc gcttctttca aacataccgg agagctgacc
    16201 gccgaccatc gcggcagccg gcgtgcagtg ttggatgttt caggcagcct gaataaggcg
    16261 aataccttac gcggaaggtt ggtgggtgcg gaagagtata aaaaatcgtt taaagaccgc
    16321 gtttggggca ggaaacatat ggtttacggc attgccgaag ccgatgcggg cgacagcagt
    16381 gtgcttactt tgggcggcat gtatcagaag agtagggagg ttcctgattt ttcgggcatt
    16441 attttgtcct gtgaaaatca gaaaactgcc ccgttcagtt caacgcctgc ctgcaaccgg
    16501 cctttgcaac tgccgcgcaa cacttatttg ggggaggatt ggtcgcggtt gagtgctgac
    16561 aaatacaacc ttttctcagg tttcaaacat gtgtttgaca acggttggca gctcaatgcc
    16621 gaagtgtctt ataccaagaa tgaatccgat gcgaaggtgg ggcagttttt tctgaaaaac
    16681 gaacatgcgg cgggtttgtc agatgaggat gcggtaggct ttttgaccga aaaaaacgaa
    16741 gtcatcccgt tcgagccgaa agataaggca ttggagaaac tgaaagcata tcgtgacgaa
    16801 accgccaagg aataccgtga gcgcaaagac gattttgtta aaaaccgttt cgataatact
    16861 gctttcgagc agtaccgcag ccgccgtgcc gcagaacgca aagccggttt tgacgagtgt
    16921 atgagtgccc cttttgcgct ggactttatc tgtcaaggtt cttgggggga tccgggtgtt
    16981 gatgccgaca agtcggaatt tgtcgataaa gcccttgcga aggaaggcat ctttaataat
    17041 gcggcacaac gttttccaaa cagcctgtat gactcttcct ttaatcggaa ggctaccgcc
    17101 aaccgacgat acagttatat gccgttgcgg cataccaaag acgaccgcca atggggaatt
    17161 aaacttgacc tgaccggcac atatgggctg ttcgggcggg agcatgattt ctttgtcggc
    17221 tatgcctacg gcgatgaaaa gatacgttcc gaatatctgg aaatctacga acgccgccac
    17281 agagtacgtc cgaatacagg ggcaacgcac ggcgtgtatg cgggaagttg tcaggggagc
    17341 cggacggtga tttgtcttct cctttggtca gggggcataa agaacccgat tggcaggcgt
    17401 acgatgaaaa aggcaaccgt accgtttatg ccgaagaatg caggaatgcc aagaaaataa
    17461 aaaccgagcc caagctcgat gccgaaggca agcaggtgta ttactatgac gaatacagcg
    17521 gcagccggac accggtatat gtcgatgtat atgagctgga cgaaaaaggc aacaagattc
    17581 aggagaccaa tcccgacggc tcgcctgtct ttaccggttt ttccggtacg gtgccggttt
    17641 ggaaaactgt taaagtggcc gatgaccatg ttcctgcgct gtataactac gccaaatacc
    17701 tcaacaccaa caaaacccat tcgctgactg ccagcacgcg tttcaacgta accggccgac
    17761 tgcacctttt gggcgggctg cactacacgc gctatgaaac ctcgcaaacc aaagatatgc
    17821 ctgtccgcta tgggcagccg gcaagcgatt ttcagacagc atcgagtatt aaggcggatc
    17881 aggaccatta cacggccaag atgcaaggtc ataaattgac gccctatgca ggcatcacct
    17941 atgacttgac accgcaacag agtatttacg gaagttatac caaaatcttc aaacagcagg
    18001 ataatgtcga tgtcagtgcc aaaaccgttt tgccgccttt ggtcggcaca aactatgagg
    18061 taggctggaa aggcgcattc ttgcaaggac ggctgaatgc ttcgttcgca ttgttttacc
    18121 tcgaacagaa aaaccgcacg gtcgtcgatt ttggctatgt tcccggagca ggcggcaagc
    18181 aggggtcgtt ccaaaccgtt gccaaaccga taggcaaagt ggtcagccgt ggggcggagg
    18241 ccgagttgtc gggtgagttg aacgaagatt ggaaagtctt tgcgggttac acctacaaca
    18301 agagccgcta caaaaacgcc gccgaagtca acgccgaacg cctcgccaaa aacacaggcg
    18361 cagacccgta caacttcagc aatttcacac ccgtgcacat attccgtttc ggaacgagct
    18421 tccatatacc gaatacgggg ctgaccgtcg gcggcggcgt gtccgcacaa agcggcacaa
    18481 gcagcctgta caacatcagg cagggcggct acgggctgat agacggtttc gtccgttacg
    18541 aattgggcaa acacgctaaa ttgagcctca tcggtacgaa cttaaacgga cgcacttatt
    18601 ttgagaacaa ctacaaccgt acgcgcggcg caaacaactt ctatggagaa ccgcgcactg
    18661 tcagcatgaa actggattgg cagttttaat tgaaaaccgt agttttgcgg atgccgtctg
    18721 aaacagggta tgtttcagac ggcattttta tggaggcagg cctatcgggg cgtatatttg
    18781 gaatttgccc gatgccggca gtagaatatc ccttttatcc tcagaagagc cgatgtcttc
    18841 acgcaaaatt atccacatcg acatggacgc attctacgca tcggtagagc tgcgcgaaca
    18901 gccgcatttg aaagggcggc cggtggtcgt cgcgtgggag ggcgcgcgtt cggtgatttg
    18961 cgccgcatcg tatgaggcac ggcagttcgg gctgcattcc gcgatgtcgg tggcaacggc
    19021 gaaaaggctg tgtccgcaag cggtgtatgt gccgccgcat ttcgatttgt accgtcaggt
    19081 ttccgcgcag attcacgccg tattcaggcg ttataccgat ttaatcgaac ccttgtcgct
    19141 ggacgaagcc tatcttgacg ttacccgtaa tttcaaaaac atcccttacg acagtgaagt
    19201 tgccaaagaa atccgtgccg ccatttttgc ggaaacgggt ttgaccgcat ccgcgggcat
    19261 cgcgtcgaac aaatttctgg cgaaaatcgc gtcggactgg cgcaagccga acgggcagtt
    19321 tgtgttgccg ccgcacaaag tcatggcatt tttggaaacc ctgcctttgg gcaaaatccc
    19381 cggcgtgggc aaggtaacgc tgaaaaaaat gcagtcgctg ggtatgcaga cggcgggtga
    19441 tttgcgccgt ttcgagcgcg gcgagctctt aaaccatttc ggacgctacg gctaccgcct
    19501 ctatgatttg gcgcgcggta cggacgaacg ccccgtcaaa gccgaacgcg aacgcctcca
    19561 aatctccaca gaaatcaccc tgcccgaaga cctgccgctc gagcaggctg ccggacactt
    19621 gccccatctt gccgaagact tgtggcggca aatcacgcgc aaaaacgtcg aagcccaaag
    19681 tgtaacgctc aagctgaaga cctacgattt ccgcatcatc acgcgcacgc tgacttattc
    19741 ctccgtattg cccgactgcg cctccctgct tcaggcggcg caaatgttga tggcgcgcgt
    19801 cccgccgcag acggaagacg cgttccgcct tatcggtatc ggcgtggggc atcttgtgcc
    19861 gaaaaaccag cagcaggatt tgtgggcgta aaccgcttta cgcgcgccgt ccaaaaaata
    19921 tgccgtctga agccttcaga cggcattgcg gctccggttt aatccgccgc gccgcttttt
    19981 tttaggttgt cgagccttcc gattatatcg gcaaggttgc gcctgccttc ttccttgctg
    20041 acaatcggtt cgccgccttt gcgccccagg attttcaaat cttcctgcgg catttcgtct
    20101 atgaatcggc tgggttcggg gaactgccat gtgccttgtt ttttgcgttt gacgcagtgg
    20161 gtcagtgtga gttggcgttt ggcgcgggtg atgccgacgt acatcaggcg gcgttcttct
    20221 tcgacgttgc cttcttcgat gctgtcgttg tgcggcaaaa cgccttcttc gcaaccgaca
    20281 aggaaaacat acggatactc caaacctttg gcggcgtgta gcgtggatag cgagacggca
    20341 tcggtttctt cttcgtcttt tccttccaaa agcgtcatca aggcgacggt ttgggcgagt
    20401 tcgatgatgt ttttgccgtc ttccccgcct tttcgcgcaa accatgatac caaatcgccg
    20461 acgttgcgcc atttgatttc gccagctttg ccttcttcac tggcaagcag gtggttttca
    20521 tagtcgattt cttcgagcag gctgttgatg aactcgcccg cttcgccggt ttcggctttg
    20581 gcacggtaac tgccgaacat atccataaag gcttgcaggt gttggcggtt ggtattgttc
    20641 agcgtggcga gggcttcttc gttttgcgcg gcttcataca ggctgcattc gtgttcgtgc
    20701 gcgtaagtgt tgagcttgcc cagcgttaca tcgccgatgc cgcgtttggg cgtggtaacg
    20761 gcacgcagaa aggcgggatc gtcgttgggg ttggcaagca gccgcacata agacaacacg
    20821 tctttgattt cggctttgtc gaaaaagctt tgtccgccgg agagacggta ggggatgcgc
    20881 gcgccgcgca atgcttcctc gaaaatcctc gcctgatgct ttccgcggta taacacggcg
    20941 aaatcggcat attgggtttt gtcgccgccg atgagttttt gtttgacgat ttggctgacg
    21001 acccagtcgg cttcgtgctg ctcgttttgg caggcaacga ctttgacggg ctcgccttcg
    21061 cccaatttcg accaaagttt tttggtgaac agcttggggt tgttctcgat gactttgttg
    21121 gcgattttga gaatccgcgc ggtggagcgg tagttttgct ccagtttgat gaccttcatc
    21181 tgcggatagt tttcctgcat tttgcgcaga ttttccatgt ttgcgccgcg ccatgcgtag
    21241 atggactggt cgtcgtcgcc gacggcggta aacatacctt ccgcgccggt cagaagtttc
    21301 atcaacgtaa attggcaggt attcgtatct tggcattcgt caaccagcag ataatgcagc
    21361 cgccgctgcc atttgttgcg cacttcgctg ttttgctgca acagcacggc aggcaggcgg
    21421 atcaagtcgt cgaagtccac tgcctgatag ctttgtaagg tttcctgata gctcgcatac
    21481 acgcgtgcgg tttgttgttc ccacgcatta gaggccgtct gaacgacatc ttcaggcgtt
    21541 ttcagatcgt ttttccacag ggagatttga tgttgcgcct tgaatacagc ttctttgccc
    21601 gtaccgccta agagttcgcc gatgattttg gcgctgtcgg tggaatcgag gatggagaag
    21661 ttttttttgt aaccgatatg gttcgcctct tcgcgcagaa tcttcatgcc caaagaatgg
    21721 aacgtgcaaa ttgtcagccc gcgcgtttgc gatttgggca gcattttggc aacgcgctcc
    21781 tgcatttccg cagcggcttt gttggtaaag gtaattgcgg caacggtatg cggcaggtag
    21841 ccgacattga caatcaaatg cttaattttt tgagtaatca cgccggtttt gccgctgcct
    21901 gcaccggcaa ggacgagcag tgggccgccg aggtagcgga cggcttcgag ctgttgggga
    21961 ttgagtttca tcatgttttg atgccgtctg aaatcagtct gcgccgcttt cgaggcagtc
    22021 gagtgtcgca cggagggcgg atacgccgat ttgccccggc gcggagtttt gcgttcccga
    22081 accgaacgtg atgcttgagc cgaacacctg tccggcaagc cggctgaccg cccccgtctg
    22141 ccccatcgac atcgtaacaa tcggtttggc ggcaagctct ttcgctttga gcgtggcgga
    22201 aagcaaagtc agcacatctt ccgcgctttg cggcatcacc gcaattttgc agatgtccgc
    22261 gccgcagtcc tccatctgtt tcagacggca tacgatttct tcttgcggcg gcgtgcggtg
    22321 aaactcatga ttgcagagca gggcggcgat gccgtttttt tgagcatttg ccacggcgca
    22381 ccggacggcg gtttcgccgg aaaacagctc gatgtcgatg atgtcgggca ggcggctttc
    22441 gatcagcgcg tcgagcagtt caaaataata atcgtccgaa cacgggaacg agccgccttc
    22501 gccatgccgt ctgaacgtaa acagcagcgg cttgtcgggc agcgcgtcgc ggacggtctg
    22561 cgtgtggtgc aatatttcgc cgatactgcc cgcgcattcc aaaaagtcgg cgcggaactc
    22621 cgcaatatcg aagggcatat ttttgatttg ctcaagtacg gcggaaagtt cggcggcatc
    22681 gcgggcgaca agcggcacgg cgattttggt gcgtccgctt ccgataacgg tatttttgac
    22741 aacaaggcag gaacacataa aacaggcaca cacagaaaaa acagccgcca ttataacaaa
    22801 gccggccgcc ttttagacgg catccagatt ccattccggc acaagccgcc gcgtcctgcc
    22861 gcatatcggc aagcaaggaa atatgcgata atggcaacct cgtgaagcag cattaccgat
    22921 agcccgcaca tcgggaaaac gatacacatc ccgcgccgca gcccgtgttg cgccgcatcc
    22981 cacataccgc atttgttccg gagtaacccc aatgtcagac gacaaaagca aagcacttgc
    23041 cgccgcactg gcgcaaatcg aaaaaagttt cggcaaaggc gccatcatga aaatggacgg
    23101 cagccagcag gaagaaaacc tcgaagtcat ttccaccgga tcgctcggat tagacctcgc
    23161 cctcggagtc ggcggtctgc cgcgcgggcg catcgtcgaa atcttcggcc cagaatcctc
    23221 cggcaaaacc accctctgcc tcgaagccgt cgcccaatgc cagaaaaacg gcggcgtgtg
    23281 cgcctttgtc gatgccgaac acgccttcga tcccgtttac gcccgcaaac tcggcgtaaa
    23341 agtcgaagag ctttacctgt cccagcccga taccggcgaa caggctttgg aaatctgcga
    23401 cacactcgtc cgttcgggcg gcatagatat ggtagtcgtc gattccgtag ccgcactcgt
    23461 ccccaaagcc gaaatcgaag gcgatatggg ggacagccat gtcggactgc aggcgcgcct
    23521 gatgagccag gctttgcgca aactgaccgg acacatcaaa aaaaccaaca cgctggttgt
    23581 gttcatcaac caaatccgga tgaagatcgg cgtaatgttc ggcagccccg aaaccaccac
    23641 cggcggcaac gcgctgaaat tctattcttc cgtccgcctc gacatccgcc gcaccggatc
    23701 catcaaaaaa ggcgaagagg tattgggcaa cgaaacccgc gtcaaagtca tcaaaaacaa
    23761 agtcgccccc ccgttccgtc aggcagagtt tgacatcctc tacggagaag gcatcagttg
    23821 ggaaggcgaa ttgatcgaca tcggcgtgaa aaacgacatc atcaacaaat ccggcgcgtg
    23881 gtacagctac aacggcgcga aaatcggtca gggcaaagac aacgtccgcg tctggctgaa
    23941 ggaaaatccc gaagtcgcca atgaaatcga cgcaaaaatc cgcgccctca acggcgtaga
    24001 aatgcacatc accgaaggga cgcaggacga aaccgacggc gaacgcccag aagaataaaa
    24061 cttaaaatcc cgataaacgg tacttctgtt gcgaagtacc gttttttttg agcagcctcc
    24121 gaacggcttg atttgagttt tg
//
LOCUS       DAWHXS010000018        24120 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWHXS010000018 DAWHXS010000000
VERSION     DAWHXS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.018"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            24..431
                     /locus_tag="WIE22_000556"
     CDS             24..431
                     /locus_tag="WIE22_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ4126695.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(782..1096)
                     /locus_tag="WIE22_000557"
     CDS             complement(782..1096)
                     /locus_tag="WIE22_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ4126696.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(1107..2792)
                     /gene="rpsA"
                     /locus_tag="WIE22_000558"
     CDS             complement(1107..2792)
                     /gene="rpsA"
                     /locus_tag="WIE22_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ4126697.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(2947..3603)
                     /locus_tag="WIE22_000559"
     CDS             complement(2947..3603)
                     /locus_tag="WIE22_000559"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ4126698.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSKLAKKLPAVFSGSRILLDGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            complement(3847..4893)
                     /locus_tag="WIE22_000560"
     CDS             complement(3847..4893)
                     /locus_tag="WIE22_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ4126699.1"
                     /translation="MAYYMVLGGWVISYIVNIIGGNLDISSPVSSEVTKSFFTEHIEN
                     SPWEIAFYTFLFVVVNQWILVKGVIGGIEKAAKYLMPLLFLFLIAMVVRNVTLPGAME
                     GITFYLKPDFSKITAELFVFVLGQVFFTLSLGFGVMITLSSYLDKNENLVQTAVITAI
                     TNTIIAVLAGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLFLI
                     AALTTSLTIYEVLITTIQEKTKIRRTAAITIVLSAIFIFGNIPSILSYGPWKDVSIFG
                     KNIFDAFDYISGNILFMLTALGSALFVGFVMKDEAKDELLYKGNHITVNIWFAYVKYL
                     VPLVILLIFVSNLF"
     gene            complement(4863..5159)
                     /locus_tag="WIE22_000561"
                     /pseudo
     CDS             complement(4863..5159)
                     /locus_tag="WIE22_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            5485..5591
                     /locus_tag="WIE22_000562"
                     /pseudo
     CDS             5485..5591
                     /locus_tag="WIE22_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5793..6485)
                     /locus_tag="WIE22_000563"
     CDS             complement(5793..6485)
                     /locus_tag="WIE22_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ4126700.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(6553..8529)
                     /locus_tag="WIE22_000564"
     CDS             complement(6553..8529)
                     /locus_tag="WIE22_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ4126701.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASRSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            8708..9373
                     /locus_tag="WIE22_000565"
     CDS             8708..9373
                     /locus_tag="WIE22_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ4126702.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            9425..10252
                     /gene="mutM"
                     /locus_tag="WIE22_000566"
     CDS             9425..10252
                     /gene="mutM"
                     /locus_tag="WIE22_000566"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ4126703.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPYRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            10340..11107
                     /locus_tag="WIE22_000567"
     CDS             10340..11107
                     /locus_tag="WIE22_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ4126704.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(11419..11730)
                     /locus_tag="WIE22_000568"
     CDS             complement(11419..11730)
                     /locus_tag="WIE22_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ4126705.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFHSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            12259..14538
                     /gene="nrdA"
                     /locus_tag="WIE22_000569"
     CDS             12259..14538
                     /gene="nrdA"
                     /locus_tag="WIE22_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ4126706.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            14596..15606
                     /gene="dcm"
                     /locus_tag="WIE22_000570"
     CDS             14596..15606
                     /gene="dcm"
                     /locus_tag="WIE22_000570"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4126707.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KK"
     gene            15603..16790
                     /locus_tag="WIE22_000571"
     CDS             15603..16790
                     /locus_tag="WIE22_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ4126708.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            16851..17984
                     /gene="nrdB"
                     /locus_tag="WIE22_000572"
     CDS             16851..17984
                     /gene="nrdB"
                     /locus_tag="WIE22_000572"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ4126709.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            18182..18472
                     /locus_tag="WIE22_000573"
     CDS             18182..18472
                     /locus_tag="WIE22_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ4126710.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(18513..18791)
                     /gene="ftsB"
                     /locus_tag="WIE22_000574"
     CDS             complement(18513..18791)
                     /gene="ftsB"
                     /locus_tag="WIE22_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ4126711.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(18805..20091)
                     /gene="eno"
                     /locus_tag="WIE22_000575"
     CDS             complement(18805..20091)
                     /gene="eno"
                     /locus_tag="WIE22_000575"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ4126712.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            complement(20139..20579)
                     /locus_tag="WIE22_000576"
     CDS             complement(20139..20579)
                     /locus_tag="WIE22_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ4126713.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            complement(20594..21436)
                     /gene="kdsA"
                     /locus_tag="WIE22_000577"
     CDS             complement(20594..21436)
                     /gene="kdsA"
                     /locus_tag="WIE22_000577"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ4126714.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(21458..21841)
                     /locus_tag="WIE22_000578"
     CDS             complement(21458..21841)
                     /locus_tag="WIE22_000578"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ4126715.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(21990..>24120)
                     /gene="mfd"
                     /locus_tag="WIE22_000579"
     CDS             complement(21990..>24120)
                     /gene="mfd"
                     /locus_tag="WIE22_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ4126716.1"
                     /translation="AIAVITESDLYQYVARSRAHNRRKKHAAVSDGLLRDLAEINIGD
                     PVVHEEHGIGRYMGLITMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHEN
                     IALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINESDYQAFADGF
                     GYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAVL
                     APTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHKL
                     VQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLR
                     DFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERL
                     ETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINR
                     ADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAM
                     QDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIK
                     LHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIES
                     HHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRFT
                     AEMENIEVRINTVKNVLKTLQNRCLPK"
BASE COUNT         5798 a         5401 c         6570 g         6351 t
ORIGIN      
        1 gttgttgttt agaaggaggg aaaatgactt ataccactgc caaagccgcc gaaaaaatag
       61 gcatctccgc ccacacccta cgtttttacg acaaagaagg tttgttgcct aatatcggac
      121 gtgatgaata cggtaaccgc tgttttaccg ataacgattt gcaatggttg ggcttattgc
      181 aatgcttgaa aaatacggga atgagcttaa aagacatcaa acgctttgcg gaatgtaccg
      241 tcattggcga cgataccatt gaagaacgcc tttccttgtt tgaaaatcaa atagaaaatg
      301 tgaagtgtca aattgccgaa ttaaaacgct atttagattt gcttgaatac aaattggcgt
      361 tttaccaaaa agcgaaagca ttaggctcgg taaaagctgt aaatttgccg caaattcctg
      421 aaacggctta gttttagtgc aataagcagg atttttgtcc tgttcgtaat agaaatcaaa
      481 aatttgttgt ttaaaaagcg gattgtaagc aatgatatag tggattaaca aaaatcagga
      541 caaggcgacg aagccgcaga cagtacagat agtacggaac cgattcactt ggtgcttcag
      601 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta
      661 atccactata aaaaaatcca cgctctaatc cgttgcttgc ttggctcaag gtattgaagc
      721 agcggatgaa gttaaaccac agaaaaaacg ccgcgtaaca gcggcgtttt gatgctaagg
      781 tttaattggc attttctttt aaagccaagt cgacccgctc gcgcaattct ttaccgggct
      841 tgaagtgggg tacatgtttt tcaggtactt ccacacgctc gccggttttg ggattgcgac
      901 cgatgcgggc aggacgatgg ttcaaatcga agctgccgaa accgcggatt tcgatgcgtt
      961 gacctcgggc aagcgatcta gtcatggtgt caaccaagac ttttacgctg tactctacgt
     1021 cttttgccag aagatgcgtg ccgtttttgg cggcaaacac ttctgccaaa cgaaccatta
     1081 actcagactt tgtcatgtct gcaaccttat tcttgttcgc cggagagttt ggctttcagc
     1141 aggtcgccca agctggtggt gccggcattc gcattggcgg cggcattgac ggagttcagt
     1201 gcttcgcggc tttctttggc atctttggct ttaacggaaa gtttgatgct gcggtttttg
     1261 cggtcaacgg taacgatgac ggcttcaact tcgtcgcctt ctttcagttt ggtggtcaaa
     1321 tcttcaacgc ggtcggctgc aaattcggaa gcaggcaggt agccttctac ttcgtcagac
     1381 agggcgacaa cggcgccttt cgcatcgaca gatttcacgg aacctttaac caaagaacct
     1441 ttgtcgttca cgctgatgaa gttgccgaac ggatcgcctt ccagttgttt gatacccaag
     1501 gagatgcgtt ctttttccac gtcgattgcc aatacgacgg cttcgacttc ttcgcctttt
     1561 ttgtatttgc gtacggcttc ttcgccggat tcggtccagg acaggtcgga caggtgaacc
     1621 aaaccgtcga tgccgccggg caggccgacg aatacgccga aatcggtaat ggatttaacc
     1681 gcgccggaga ttttgtcgcc tttgttgtgg ttggcggcaa attcttccca aggattggct
     1741 tggcattgtt tcatacccaa agagatacgg cggcggcctt cgtcgatttc caaaatcatg
     1801 acttcgactt cgtcgcccag ttgtacgact ttgctcgggt gtacgttttt gttggtccag
     1861 tccatttcgg agacgtgtac caaaccttcg atgccttgtt cgatttcgac gaatgcgccg
     1921 tagtcggtca ggttggatac tttgccgaac aggcgggtgc cttgaggata acggcgggtc
     1981 agaccgctcc aaggatcttc gcccagttgt ttcataccca aggaaacgcg ttgtttttct
     2041 tggtcgaatt tcaatacttt ggcttcaact tcctgaccga cttccaagac ttcactcggg
     2101 tgtttcacgc gccgccatgc caaatcggtg atgtgcaaca gaccgtcgat gccgcccaag
     2161 tcaacgaatg caccgtaatc ggtaatgttt ttaacgatgc ctttgatgac ggagccttct
     2221 tgcaggtttt ccagcagggc tttgcgttct tcacccaaag tggcttccag aacggcgcgg
     2281 cgggaaacaa cgacgttgtt gcgttttttg tccagtttga tcactttgaa ttcgatctct
     2341 ttgccttcga agtgagaagt gtcttttaca ggacgtacgt cgaccaaaga acccggcagg
     2401 aatgcgcgga tgctgctaat cataacggtc aggccgcctt tgactttgcc gttgatgatg
     2461 ccggacagga tgttgccgtt ttccatggct tcttccaggg caatccaatc ggcagcgcgt
     2521 ttggcttttt cgcgggacag tttggtttcg ccgaagccgt tttcgacgga ttcgatggta
     2581 acggtaacga agtcgccgac tttaacttca atttcgcctt gagcgttttt gaattcagct
     2641 acatcaatca gggattctga tttcagacct gcgtttacgg taacgaagtt ttggtcgatt
     2701 gccactactt cagcggtaat cacctcaccc gggttcattt cttgcagggt aaagctttct
     2761 tccaacagct gagcaaaatt ttccatagac atatataact cttttcggta caccgccaag
     2821 gggtgcgggg tgggttggtg ggtgtctgcc gtccttggca gggcagacgg gtaaataagg
     2881 tttcagacgg catatgggga aattcatgct gtctgaaacg caatgcgggc gattatacct
     2941 gaaaatttaa actttacgat accaatcaag cacttttttt acagtttctt ctatagtcag
     3001 gcggctcgta tccaaaagca gggcatcggg ttgttgtttc aggggggcaa ccttgcggtt
     3061 tcggtctgcc tcgtctctgg cttcgatgtc ggacaggatg cgctcgaatg ccaaaccttc
     3121 gcaggggatg ccgatttgtt tggcgcggcg ttcggccctg attttggatt cggctgtcag
     3181 aaaaattttg agttcggcct gggggaagac gaccgatccg gtgtcccgtc cgtcggcgac
     3241 cagtcctttt tcggtcagaa aatcgcgttg gcgttgcagc agggaggcgc ggactttagg
     3301 caactgtgcg actgcggatg cgcccatgcc gatggcttct gtccggatgc cgtctgaaac
     3361 gtcttcgccg tcgagcagga tgcggctgcc tgaaaatacg gcgggcagtt tttttgccag
     3421 tttggaaacg ttttcttcat cgtgccattc cacgccttgt ttttgtgcat atagggcggt
     3481 caggcggtag agtgcgccgg tatcgagata atcgtatccc aatgcggcgg caacgcgggc
     3541 ggcaaccgtg cctttgcccg atgcgcccgg gccgtcgatg gcgatgactt tttgtctgtt
     3601 cataaggggg attcctgatg gtttggggta tgggttttgc cgtctgaagg atgtgtttcc
     3661 cgttggggca gattctacct gttttaaagg acgattgtct aagcagacgg aacgccgcct
     3721 gcccggaaat atccgacaga agccggcaag ccggttcgga ttctggcgtt gcgggtattg
     3781 tggcgggcat caacgcaatg ccgtctgaaa gaggtatgac tttcagacgg catcgattgc
     3841 tgcggattag aacaggttgc tgacgaaaat cagcaaaatc accagcggca caaggtattt
     3901 cacataggca aaccaaatat tgaccgttat atggttgcct ttataaagca attcgtcctt
     3961 cgcttcatcc ttcatcacaa aaccgacaaa cagcgcggag ccgagcgcgg tcagcataaa
     4021 caagatgttg ccgctgatgt agtcaaaagc gtcaaaaata tttttgccaa atatggaaac
     4081 atctttccac gggccatagc tcagaatgga cggaatattg ccgaagatga agatggcgga
     4141 caatacaatc gtgattgcgg cggtacggcg gattttggtt ttttcctgaa tggtcgtaat
     4201 caacacttca taaatcgtca gcgaagttgt cagcgcggca atcaggaaca gagagaagaa
     4261 aatgacagcg aacacaggtc ctgcccacat atgtgagaac acaatcggca aactttggaa
     4321 taccaaagtc gggccagaat tgggcgcgac gccgaagctg aagagcgacg ggaaaatcat
     4381 aaagccggcc agtacggcga tgatggtatt ggtaatggcc gtgatgaccg cggtttgcac
     4441 cagattttcg ttcttatcca gatagctgga caaggtaatc atcacgccga aacccaagct
     4501 caaggtaaag aacacttgtc ctaaaacgaa gacgaacagt tcggctgtaa ttttgctgaa
     4561 gtcaggtttc agatagaagg taatgccttc cattgcgccc ggcagggtaa cgttgcgcac
     4621 aaccatggca atcaggaata aaaacagcaa aggcatcagg tattttgccg ctttttcaat
     4681 accgccgatc acgcctttta ccaagatcca ttggttcaca accacaaata ggaaggtgta
     4741 gaaagcaatt tcccaaggac tgttttcgat gtgttcggta aagaaacttt tggtgacttc
     4801 gctggaaaca ggactggaaa tgtccaaatt accgccgatg atattaacaa tgtagctgat
     4861 aacccagccg ccgagcacca tgtaataagc catgatgccg aacgcgccga gcagcccatc
     4921 cagccgacca gtttccaaat tttggcaatg ggtttgccgt tcatcgggcc gccgaacgca
     4981 tccagcgcgt tcacgccttt gcgccgtccg atgacatttt ccaccaaaat catcgggatg
     5041 ccgataacca gcatcgcgat acagaataaa aacacatacg cgccgccgcc gttttcgccg
     5101 acaaggtagg gaaaacgcca cgtcgcgccg aagccgacag tcgcgccggc aacggtcagg
     5161 atataggtta atcggctgga tcaggtttga cgattggtat ttgaagggga agacatattg
     5221 aaaccgtgtc cgattgagat aaagcggaaa ttctacacgc gttttttaac aggaactgac
     5281 tgattgtttt tcaactgtct agtgattttc catgtaaaag gcatatattt cagtgagtat
     5341 ttctgatgag tataacccga tgaggaagaa tcggagttta taaatagatt aatttgttat
     5401 tcttctacat cggtgtatgg aaatgaaatt ttgttaatta tattaatgat agcatttaaa
     5461 taatgatgaa agagagggaa tttgaaatat agtggattaa caaaaatcag gacaaggcga
     5521 cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgctgtac tggtttaaat
     5581 ttaatccact ataaaaaaac catctataca aggggagaag tatagatggc aaaacacatt
     5641 acggtgaaaa cgtcttactc ataagcctgc ttgaacaggc gttactcaga caaatggatt
     5701 atatcgtaaa ttgatttttt gcgttaaatt gggttaaacc atatatttaa tgagtatgtg
     5761 gatgaatata aatggaattc ttggatgtta tttcagaatt ttggacattc gataaatttc
     5821 cattccccgg gtttgaggtt ttctgtttcc agatgtgcga atcgccggcg atgaaggtgt
     5881 tgcacgcggt tgccggcggc ggcgatcatg cgtttgactt ggtggtattt tccttcggta
     5941 atggtcagca gcagggtggt cgggtttttc aaaacggcat cggcggcaca aacggtttcg
     6001 ttttcgtcgt ggagcagcac gccgtttttc aaggtttcgc agagcgtttc tcctgtgggg
     6061 tgtttgagcg ttacttcgta tagcttggga atttttctgc tcggcgaagt caggttgtgg
     6121 ttcagtttgc cgtcgttggt aatcagcaat acgccggtcg tatccgcatc cagcctgccg
     6181 accgcctgca tatcgatgtt ccgcatattg tcggggaaca ggctgaatac gctgcggtag
     6241 tgcttgggtt tgtgcgaagt ttcgtaatct tcaggcttgt tgagcatgat gtagaaatag
     6301 ggttcgggaa cgacggttac tgcttccccg tcaatatcca acgtttcgac ggatgaggaa
     6361 tcgatgtctg catcggtgtc gtccatgcag gttccgttga tgaaaacata accgccggca
     6421 atcagccatt ggcactgctt gcggcttcct atgccttgat attgcaggta tttgataagt
     6481 ttcatgatgg tatgggaaat gtgggatgaa aaagacagga ctgagaaagt cctgtcatgt
     6541 gccgtatccg aatcagcttc caatcagttt gaccctctgt cccggattga tggtgttcag
     6601 gttgggattg agccggcgga tgtcgtcgat atggatattg aagcgcgcgg cgatactttt
     6661 gaaggtgtcg cctttgcgcg cggtgtagga tactttttca atacgggttt gggcaggggc
     6721 tgcctgcgcc aggcggagta cctgtccttt ttggatggtg ttgcctttga tgttgttggc
     6781 gacaatcagg tcggctacac tgacgttgta gcgtttggca atgttgaaca gggtgtcgcc
     6841 ttctacaacg cggtggatgc tggcatggag cggggagatt tcggcttcgc cgtcggcagc
     6901 tttggctacg cgcgtttcca aacgtacccg gcgtttgccg tcggcggcaa cggtatgttg
     6961 cggagatgag gaaatgttgt gttcggcaac tgtgtcaggc gtgccgatga cggcggagat
     7021 ggtttcttca gcctgtcggc gcaggttgtt tcgggcaaca agctgcatga gttcgtctgc
     7081 cgcctgcatg tcgttttggg ggatgatctg cgcgagtgtt gcggtttggg tttcagacgg
     7141 catggcggtc tgtttttcgg tttgaggttg cgctgcggct gtcgcaaggg caggttcggc
     7201 aatacggaca aggggatcgg gttccgtacg gacggttttc tgcggcaaag gtgcgacggt
     7261 aatgtccgct gtctgtgcgg cggcgggtcg gattcgggca atgccgacgt tcaccgtgcc
     7321 tgccggcata ttggaacggt aggtgtcggg tgtattgtcg atgtcgatgg aaacgacgga
     7381 ttccgatgcc gtctgaaggg ttttgccgtt cttggcgaca aggatgctgc gtcctgcgtt
     7441 gacaaggttg ccgttcaggt tgttgaggcg tttgatgtcg gcaatgctca tgccggttgc
     7501 cgtcgagatg tcggacaggc tggttttggc ggcaggcgta tagacttccc atgaaaacag
     7561 gctgtcgggt gcggcgttga ggtagttgct ttggaaggtt tgtacggacg cgacaggaag
     7621 cagcagtttg cgtttgcttt tggggataaa cgcggggacg ttgaatgcgg ggtttagggc
     7681 gagcagctcg ctttgcgtga tgccggcaag ccgggcgatg gcttcgttgt cgagcggacg
     7741 atccggttcg actgcctgaa aatagggttt gttgtctatg tcgctgatat tcatgccgaa
     7801 agattgggga gtggcaataa tgttgcgcac ggcgagcagc ttggggacat agttgcgcgt
     7861 ttcgttgggc atacgcaggt tttcgtaggt cggttcgagc ccttgggcgc gggcgcggtt
     7921 gatggcgcgt ccgacgttgc cttcacccca gttgtaggcg gcaaaggcaa gcggccagtc
     7981 gccgaacagt ccatagaggt attgcagata gttgagtgcg gcatcggtgg cggcgtaaac
     8041 gtcgtgcctg ccgtcgtaaa ccggtgtttt ttccaggccg taatgcctgc cggtagcggg
     8101 cataaactgc cataatcctg atgcgccgac gtgtgatttg gctttggtga cgaacgcgct
     8161 ttcgatgaag ggaagcaggg cggcttcggc gggcatattg cgttttttga cttcgttggc
     8221 gatatggtac atatagggtc tactccggtt gatgaccctg ttgaaatagc tgcggcttgc
     8281 gatgaatttg ctttcgtggc ggcgtaccag ttcgggattg acttcgccca tccggaagcc
     8341 ttggcgcagc tcgctccaca ggctgccgga ttggaaatat tgttttgtcg ggggcaggtc
     8401 gagtattgaa gagtttaagc gcataatcgc caaaccgatt tggtgtgatg aggtgttttg
     8461 ggcgtatagg aaacccggac aaacggacag acctgatgcg gtcagagcga tggttttgag
     8521 tttggacatg atttatattc ggcggaataa acatatttta tcgtccgatg gtactgtttg
     8581 actgaaaaag cgtcaagttt tacaggaact ttatgcggat tcggaaatgg gcggccgatg
     8641 tgttttttga cagaaatcct gtttggaata gaatatcggc aggcaatctg tttgaaacgg
     8701 acgcaatatg gatgcatggt ttgaagatac ggcgatgggg cggtatgttg caaaattgga
     8761 acaggatttc ttcgggcggt atctggattc ataccgtttt tcgggaatgt gtgcggttca
     8821 ggtgggcggc aggtggctga gcctgtctga agatgttgtc tgtgtgccgc gagatatgtc
     8881 gatgtcggcg gagaatatgg ctttggcgga tgtttctgcg gatatgctgc tttttccgca
     8941 tacgcttgaa ggcggtgttc cttcgcaaat cctgtcggaa gcgcaccgga tattgaaacc
     9001 gtgcggacgc ttgatgctga cgggtttcaa tccgtattcg ctctggggat tcagccgttg
     9061 gtttgacggc gaacgcctgc cggaaaaacg gttttgtctg ccgctgcccg agttgaaaag
     9121 gcggcttgcc gatgccggtt tcgatattga atttgggaaa tttatggtgt atctgccgcc
     9181 ggtttcgtcg ctcgggcaaa tacgcttttg gcggtttatg gaaaaggcgg gcgaccgttg
     9241 gtggccgcaa tgtgccgcag tgtacgcttt ggttttggtc aaaagggcgg cgggcgtaac
     9301 gcccctgccc gcgtgggacg ggtatttggg cggcaaagcc cttgcggcgg gtgcggcaag
     9361 ggttgcggat tagggacggc cgtctttcag acggcatctg tcagaaaaaa tgtcgggagg
     9421 ggttatgcct gaattgccgg aagtggaaac gacgttgcgc ggcatcgcgc cgcatattga
     9481 agggaaaacg gtggaagccg tggtattgcg ccaattgaag ctgcgctggc agattaatcc
     9541 cgatttgggg gagattttgt ccggccggca ggtgttgtcc tgcggcagga gggcgaaata
     9601 cctgcttatc cgctttcaaa cgggcgtgct gctgattcac ttggggatgt cgggcagctt
     9661 gcggattttt acgccgtcgg acggacgtat cggcaggccg gacagacacg atcacgtcga
     9721 tattgtgttt tcagacggca cggtcatgcg ttaccgcgat ccgagaaagt tcggcgcgat
     9781 actttggtat gagggaatcg aagaacatca tccgctgttg gaaaaactgg ggccggagcc
     9841 tttgtcggag gcattttgtg cggattatct gtatgcaagg ctgaaggcgc agaagcgcgc
     9901 ggtcaaactt gccctgatgg acaatgcggt cgtggtcggt gtgggcaaca tttatgccaa
     9961 cgagagcctg tttagggcgg gcatttcgcc ctaccgtcct gccaaccgcc tgaaaaagaa
    10021 agagtgcgcg cttttggttg aaaccgtcaa agcggtgttg cggcgcgcca ttgaaacggg
    10081 cggcagtacc ttgagggatt ttgtggacag cgacggcaaa agcggctact tccaacagga
    10141 atatacggtg tacgggcggc acaatcagcc gtgcccccag tgcggcggtt tggttgtgaa
    10201 agaaactttg gggcagcgcg gcacgtttta ttgcccgaac tgtcagaaat aggactgaaa
    10261 acggtttcag acggcatttt atcggtatgc cgtccgaacg tttcaacaac aaacaccgat
    10321 tatcgggaaa gaattgctca tgtcttcaaa taaagcttca ttttttacac gtctgcgccg
    10381 cttgtgccgt ttggcggtct ggctgttcaa aaccgggaaa aacctgcgcg gtattgacgg
    10441 cggttgcccc gagtcgcgca atcgggcggt aatcgagttg ggcagggggg ttttggcggc
    10501 tttggatatc ggattggagg tgggcagacc cgcacccgaa catccgaacg gtgtcttggt
    10561 tgccgccaac cacgtgtcct ggctggatat tttcgcgatg agcgcggttt atccgagcag
    10621 ctttatcgcc aagcaggaaa tcaaaagctg gccggtattg ggcaagatgg ggcagaacgc
    10681 gggaacggtg ttcatcaacc gcaattcgcg gcgcgacatc gaaccgatta accgcgccgt
    10741 ctgcgaaacc ttgcaacgcg gtcaaaacgt cagttttttc cccgaagcgc ggacttcctc
    10801 cggattgggg cttttgccgt tcaaagccgc gctgttccaa tccgccatcg atgcgggggc
    10861 aaaggttttg gcggtcgcgc tgcgttatta tgacgaaacg ggaaaaagga cggctcgtcc
    10921 ctcatatgcc gatgtcggtt tgccgacctg cctgtggcgc atcgtgtcta tgaaaaaatt
    10981 gacgataaaa gtcgatttcg tttgcgtggc ggatgcggcg gaaagcgaag accgttatgc
    11041 tttaaaagat aaaatcgaag aaagcatccg tgccgttgtc gccgacgatg cggatatcgc
    11101 tgtctgaaac cggttgtcgg aatgtggcag tatgattcgc ttttgtggat gtatagtgga
    11161 ttaaatttaa accagtacag cgttgcctcg ccttagctca aagagaacga ttctctaagg
    11221 tgctgaagca ccaagtgaat cggtttcgta ctatctgtac tgtctgcggc ttcgtcgcct
    11281 tgtcctgatt tttgttaatc cactatatta aggctgcggc aacgggagga cctcaaacga
    11341 aatggcgtaa ggatagtttg ttgtatctat ggaaaatgcc gtctgaacct gtgttcagac
    11401 ggcatatttt atatgggatt aacggcggaa actgccgccg tcgaaatcat cgaaagcctc
    11461 ataagccgcc cggcattgga ttttctgttc cgcataaggc agggagtgga atgctttgcg
    11521 cgcctttttg ttttcgcgca attcctgcac gttgccttgc gatcggtacc acgcggcacg
    11581 gcgttccaaa tatttcctgc attcggggtg cagcatctgc ttgtttgcct tgcggatatg
    11641 tttggtatcg cggtgggcgg gttctgacgt accggcagcg gcagttgtcg ataccagtcc
    11701 caatattgca gacaacagta tggcaagcat ctttttgaca gacatgacga tcctttcagt
    11761 ttgtcattcc ttccagtata ggatggaagg atggagcggg caaattgtcg gcagtttcag
    11821 actcattcag ccgacaaaac ttgccgtttg atatgcagat tcaaataaca cattaatctc
    11881 aataataaat tccaatcggt aagaaaatgg aatttgtcgg cggcggcgcg gcaaaatcat
    11941 actttgcaaa atttaacaat ttgcaggggc agaaaacagg aagctttcct ttttcgtcgg
    12001 aaaatcctta tttcaccgcc ttgtagccgg agccggtcaa aaggcaaaaa atttacccgt
    12061 tttttatcgg taaagaatta tcagataaaa caaatattat aggaaaaata cgacaggcgg
    12121 gttttatcgc gcattgcctg aaactgaaaa atacaaccgt tgtcaagact ggagaaaatg
    12181 ccaaaaatcc actatattgt ctgccttaat ttatttgaaa agaccgtgtc ttgaatatca
    12241 agagtggaag aggaagcgat gaatacaccg actgatttga aagtaaccaa acgagacgga
    12301 agattagaag ccattgattt ggataagatt caccgtgtcg tcacttgggc ggcggacgga
    12361 ttggaaaatg tttccgtatc tcaggtcgag ttgaaatcgc acatccagtt ctacaacggc
    12421 atccgcaccg acgacatcca cgaaaccatc atcaaagccg ctgccgattt aatttcggaa
    12481 gataccccgg actaccaata ccttgccgcg cgtttggcga ttttccatct gcgtaaaata
    12541 gcctacggcg agtacgagcc gccgcacctt tacgaccacg ttaaaaaact taccgatgcc
    12601 gcaaaatacg acaggcatat ccttgaggat tacagccgcg aagaatttga cgagctgaac
    12661 gcctatatcg accacgaacg cgatatgtcc ttttcctatg ccgctgtcaa acagctcgaa
    12721 ggcaaatatc tggtacagaa ccgcgttacc cgccagattt atgaaacacc gcagttctta
    12781 tatgttctgg tggcgatgtg tcttttcagc aaatatccca aagaaacacg cctcgattac
    12841 gtcaaacggt tttacgatgc cgtttccaca tttaaagtat cgctgccgac cccgattatg
    12901 agcggcgtgc gtacgcctac gcgccagttc tcaagctgtg tgctgattga atgcgacgat
    12961 agtttggatt ccatcaatgc cactaccagc gcgattgtga aatacgtttc ccagcgtgcg
    13021 ggcatcggca tcaatgccgg acgtatccgc ggtttggaca gcgaaatccg gggcggcgaa
    13081 gcgcggcata ccggctgcat cccgtttttc aaaatgtttc aggcggcggt caaatcctgt
    13141 tcgcaaggcg gcgtgcgcgg cggcgcggca accttgttct accccttgtg gcatatcgaa
    13201 gccgaaagcc tgctggtgtt gaaaaacaac cggggcgtgg aagacaaccg tatccgccag
    13261 cttgattacg gtgtgcaaat caaccgccta ctgtacaccc gcctgattaa gggcggcaac
    13321 attacgctgt tttcgcccaa cgaggttccg ggattgtacg aagcgttttt tgccgaccaa
    13381 gacgaatttg agcggctcta tacgaaatac gagcaagacc ctgatatccg caagcgcatc
    13441 attccggctg ccgacctgtt ttccacgctg atgcaggagc gtgccggaac cgggcgcatc
    13501 tacattcaaa acgtcgatca ctgcaatacg cacagcccgt tcgatccgcg cgtcgcgcct
    13561 gttcatcagt ccaacttgtg tatggaaatc gccctgccga ccaaaccgct ggacaatatc
    13621 aacgatccga acggcgaaat cgccctgtgt accctgtccg ccttcaactt gggcgcatta
    13681 aacagcctgg acgaattgga agggcttgcc gatttgactg tgcgcgcact cgatgcactt
    13741 ttagattatc agggatatcc ggtagaagcc gcgcgtacct ccactatggg ccgccgttcg
    13801 ctcggtatcg gcgtgattaa ctatgcttat tatctggcga aaaacggtgt ccgctacagc
    13861 gacggctccg cgctcggtct gacccaccgt acctttgaag ccatacagta ttacctgctc
    13921 aaagcatcgg caaaccttgc caaagaatac ggtgcgtgta cgctctttaa ccaaaccgtt
    13981 tattcgcaag gcaaactgcc catcgacacc tacaaaaaag atttggatgc cgtctgcggc
    14041 gagcctttgc attacgactg ggaaagcctg cgtgccgaaa tcgtcaaata cggcctgcgc
    14101 aactctactc tgaccgcgct catgccgtct gaaaccagct ctcaaatcgc caacgccacc
    14161 aacggcatcg agccgccgcg cggattggta acggtcaaag catcgaaaga cggcattttg
    14221 aaacaagtcg tgccggagtt tgaaaccctg aaaaatgcct atgaaaccct gtggcagctt
    14281 cccggcaacg aaggctacct gaaacttgtc ggcgtgatgc aaaaattcgt cgatcaatcg
    14341 atttccgcca ataccgccta cgacccgggc aaattcgaag gcggcaaagt ttctatgaaa
    14401 caaatgctca aagacctgct gaccgcctac aaatacggcg tcaaaaccct gtactaccat
    14461 aacacccgcg acggtgcgga cgatacgcag accgatattc aggatgacgg ctgcgcgggc
    14521 ggggcttgta agatttgatg aaagggggag ttttcagatg gcctttagat taataaatca
    14581 tctgaaatat aaaatatgaa aaataaaata caaaatcaat tagataatat agttacctta
    14641 aataatgaga aatttacttt tattgattta tttgcgggaa taggtggttt tcgcattgca
    14701 atggagaatg ttggtgggcg atgtgtattt tctagtgaat gggatgataa agcccgtcaa
    14761 acctaccaag taaattttaa tgatattcct tatggagata ttacattaaa ggaaaccaaa
    14821 gcagctattc caagtaagtt tgatgtatta acagcaggat tcccgtgtca gccattctct
    14881 atagctggtg tttcaaagaa aaaaagccta ggacgagaaa caggcttctt agataaggcg
    14941 cagggaactc tattttttga tgttgctgaa attattggaa aacatcgacc taaaattttt
    15001 cttttagaga atgtgaaaaa ccttgtttcg catgacaaag gaaatacatt taaagtaatt
    15061 aaagggactt tagaagagct tgactatcag atattttatc aagttatgaa tgcaaaatat
    15121 tatgttcctc aaaataggga gcgtattttt attgtaggtt ttgatagaca atattttaat
    15181 aaggaaataa atttcaattt tccttcccca ccagaatcac aaccaaaatt aaagcaaatt
    15241 ttggaagatg atgtagataa ttcttttact ctttctgata acttatggct ttaccttcaa
    15301 aattacgcta aaaaacataa ggcaaagggt aatggatttg gttttggatt agttgattta
    15361 gatggaatat cacgaactct atctgcacga tattacaaag atggttccga aatactcatc
    15421 cctcagaaag gaaaaaatcc taggaagctg acacctaggg agtgctcgcg tttaatggga
    15481 tttcctaaag attttgttat tgatgcagta tccaagacag ctgcatacaa gcagtttggc
    15541 aattcaattg ctgtaccgtt ggttcaagct attgctaaac aaattataaa tgagttaaaa
    15601 aaatgaatga cttggcagct cagacaatac aagtagtaaa taaatccgat gacttagtag
    15661 cttcagcaat acagacagca aataaatcaa ttgcagtata ctgtcgttat attcgtccca
    15721 atgatgttgg tactactggt agtcaccaat caggatttta tattcaaaaa catttttcag
    15781 acgacctctt tgatgtagtt tgccaaaagg gaacaaataa aactatttca atcaaaatta
    15841 attggcaaga tggaagcgtc actaatagca attttaaata ttacggccaa ggcacgagaa
    15901 atgaggcgag aattacgggt tttggcaaga attttgaatt tttaagtgat aaatatagtg
    15961 gttccttatt ggtattgtgc agagcttgtt ataaagattt attattccat gcttttgttt
    16021 tatcttcaga tgaagatatt gaaatattta tcgcagaaac taacatttta ccaggaagtt
    16081 tatatttacc taaaaaacaa gaagtagagg ataacctaac aaaattattt tcactatttc
    16141 ctaacttccc caaaactgaa gaaatggcag ttttggcaag ggaaaatatt gcattaaata
    16201 aaactaacgt tagtaatgtg ttaaaacaat gggtttcaaa ggaatatgaa ttattttcca
    16261 tttttgaaaa acgtgaattt gaaattatta aatcaaaaat tactgatttg gatagcttca
    16321 ttaattttgc acattctttt acaaatcgca gaaaagcaag agcaggaaaa tctttagagt
    16381 tacatttatc aagaatattt gacgaatttt ccttaaaatt tgagacgcaa gcgaagacag
    16441 aagggaaaaa gaaaccagat tttttatttc ctggaagtga agagtaccat gcgatggatg
    16501 aatcagggaa tttcatattt caaactgaaa agctgacaat gctaggttca aagactactt
    16561 gtaaggatcg ctggcgccaa gttttaaatg aagcagatcg aattcctcat aagcatttat
    16621 ttactctaca agaaggtata tctgatactc aaattcagga aatgagtgat gagaatttaa
    16681 ccttagttgt ccctaaagaa tccgtaaaaa catttggaac ttttgggaaa acccatgttt
    16741 taactttaga aaattttatt aaatatataa agtctcagca ggttagttaa aacttacaga
    16801 tcatttttaa ttaatataga gaatagatta tgtcctgcga acacttaact atgtcataca
    16861 gcacctttcc caaaaccaaa aacgacgcgc tgaatgagcc gatgtttttc ggtcagccgg
    16921 ttaatgtcgc ccgttatgac cagcagaaat acgaggtatt tgaaaaactg attgaaaaac
    16981 aattgtcgtt tttttggcgg ccggaagaaa tcgacgtgtc gcgcgaccgt atcgactacg
    17041 ccaacctgcc agaacacgaa aaacatattt tcatcagcaa tctgaaatac caaacactgc
    17101 tcgattccat ccaagggcgc agtccgaatg ttgctttgct gcctttggtt tcgattcccg
    17161 agttggaaac gtggattgaa acgtggagct tcagcgaaac catccactcg cgcagctata
    17221 cccacatcat ccgcaatatt gtgaatgatc cgtcggtcgt gtttgatgat attgtcgaaa
    17281 acgaatacat taccgcccgc gccgaagaca ttgcctgcta ttacgatgac ttaatcgaat
    17341 acacccagta ttacaacctg ttgggcgaag gggtgcacaa tgtcggcggc aaacccgtta
    17401 ccgtgtcttt gcgcgggttg aagaaaaaac tctatctctg cctgatgtgc gtcaatgtgt
    17461 tggaagccat ccgtttctac gtttcattcg cctgctcgtt tgcttttgcc gagcgcgagt
    17521 tgatggaagg caacgccaaa atcatcaaac tgattgcccg cgacgaagcc ctgcacctga
    17581 ccggcacgca gcatatgctt aatctgatgc gttctggtgt tgatgattct gaaatggcgg
    17641 aaattgccgc cgagttgcag gacgaatgtt tccaactctt caaaaaagcg gcggaacagg
    17701 aaaaagaatg ggcggcatat ttgtttaagg acggttcgat gatcggtttg aacaaagaaa
    17761 tcttatccca atacgtcgaa tatattacca atctgcgtat gcaggcggtg gggttgccgg
    17821 ccggatttga aggcgcaaat caaaacccga ttccgtggat taatgcgtgg ctgtcgtccg
    17881 acaacgtaca ggtcgcgccg caggaagtgg aaatatcctc ttatttgatc ggtcagatag
    17941 attctgaagt gaatacggat gatttgggcg attttgagtt gtaaatgttt tttgaaaaaa
    18001 tgccgtctga agccggatgt ttcagacggc atttgtatgt tgtatgatgg gaaaaacagc
    18061 gggtaggatc ggttattgtt ccttttatag tggattatag tggattaaca aaaatccgga
    18121 caaggcgagg caacgccgta ctggtttaaa tttaatccac tatatttttg attggtcgga
    18181 aatggcgcgc attggtacga ataagggttt ttttgagctc ttggagggcg agaccctgtt
    18241 ggaaggcttg gaacggacgg ggcatatggt ggaatatcag tgccgaagcg gatattgcgg
    18301 atcgtgccga gtgaagattt tggagggaag cgttacttat cgggagccgc ctttggcttt
    18361 tttggggcgg gacgagatac tgccgtgctg ctgttgcgtc gagggggatg tcaggctgga
    18421 ttgcggtttg tcgggggagg atgaggggct gtctgacagt ttgttgaaat aaaagccgtc
    18481 cgaacattgg ttcggacggc tttttgacgg tattaccggt tatgcctgat gagtcggtaa
    18541 aaggtttcac cgtcttggat ataacccagt tctacccggg cgatttccga aatggcttct
    18601 tgaccgtttt ccaaatcata gacttcggcg gcaagggaat gattgcgtag ggcgagtgtc
    18661 tggttttttt cttcttgaac ggcaatctgt tctctcagac tgctgttgcg tccgatgctg
    18721 cctttgccga accagaggct gtattggcaa cagacaagtg cgaaggataa aacgacagtt
    18781 acccacttca taccttaacc ttttttattt gcccagttgg tagaatgcgg ctttgccggg
    18841 gtagtcggcg gcttccgcca attcttcctc gatacgcagc agttggttgt atttcgccat
    18901 gcggtcggaa cggctcaaag aaccggtttt gatctgcata cagttggtgg cgactgccaa
    18961 gtcggcaatg gtgctgtctt cggtttcgcc ggagcggtgg ctcatcacgc tggcgtagcg
    19021 gttgcgtttt gccagatcga cggctttcag ggtttcactc aaagtaccga tttggttgac
    19081 tttgaccaat agtgcgtttg ccacgccttt ttcaatgcct tcggcaagga ttttcgggtt
    19141 ggtaacgaag aggtcgtcgc caacgagttg gactttgccg cccagttttt cggtcagcag
    19201 tttccagcct tcccagtcgt tttcatccat gccgtcttcg atggagatga tggggaactc
    19261 gttgaccaga ctttccagat attcggcaaa ttccgcgttg gtgtaggagc ggccttcggc
    19321 ttccaagtgg tatttgccgt ctttgtagaa ctcgctggag gcgcagtcca atgcgaataa
    19381 tacgtcttcg cccgctttgt agccggcggc ttcggtcgcc tcgaccatca gttgcagggc
    19441 ttctttgtgg ctgttcaggt tgggggcgaa accgccttcg tcgccgactg tggtcgggaa
    19501 gcctttgctg tcgcacagtt ttttcaaggc gtggaaaatt tccgcaccgc agcgcaacgc
    19561 ttcgcggaaa gattttgcgc cgacgggcat aatcataaac tcttggatgt tcaggctgtt
    19621 gttggcgtgt tcgccgccgt tgatgacgtt catcatcggt acgggcaggg acatcgggcc
    19681 tgcgccgccc aagtagcggt aaagcggcag gcctgagtct tcggcagcgg cgcgtgcaac
    19741 cgccatagaa accgccaaag tcgcattcgc acccaaattg cctttgtttt cagtaccgtc
    19801 caattcgatc atgatttggt cgatataaga ttgctcgttg gcatcgatac caatgagggc
    19861 ttgggcgatt tggttgttga cgtgttcgac cgccttcaat acgcccttgc ccgaataacg
    19921 ggatttgtcg ccgtcgcgaa gttccaaagc ctctttttga ccggtggacg cgccgctcgg
    19981 tacggctgcg cgtcccatta cgccggattc gagcaataca tcacactcga ctgtggggtt
    20041 gccgcgtgag tccaaaattt cgcgggcgaa aatatcaacg attgcgctca tgaatgttct
    20101 ccaagtgaag tgaagggagg taaaaaaatg ggagggcttc agacggcatc tttgccggaa
    20161 agccgccaac ttaaaactgc tgaaagtagg gcggttgcca tgaaaatcat gctggaaata
    20221 ccgtgccaca ttccgaagga tccgcctaca aacgacaaca gccaattttc atgcccgtat
    20281 ttcagtgcct cgataacggg ggtaaccaaa aattggttgg cggcaagggc ggacaataaa
    20341 aaaagcagca gggcggtttg cagtcgggtc aggatggcaa aggcggcagc caacaccgcg
    20401 ccccaaacgg caagcccgct ccaagagagg atgtcgaaca atacgccggc aatttcgccc
    20461 gcctgcattt tgggcagcat tttgaacagc accggtgcgg cgatataacc cgccataatc
    20521 tgcataccga gccacaaggt tgcgacatac cggctgattt ttcggaaagt ctgcatcata
    20581 aggcgaaacc gtgttactcg attgttaaaa tcggttgtga tttgattaaa tcgtccaatg
    20641 ctttgatgcg gattaaaaaa tcttctaaaa ggtgcagcgg cagcgcgctg gggccgtcgc
    20701 attttgccag tttcggatcg gggtgcgatt cgaggaacag accggcaagg cgggttgcca
    20761 tgcctgcaag tgccaaatcc aaagcctgtg cgcgacgacc gccggatgcg gcagaaccgg
    20821 catcgcgggt ttgcagggaa tgggtaacgt cgaaaataac cggcaggttg ccgcaagtct
    20881 gtttcatcac gccgaaaccg agtatatcga caacgaggtt gtcgtagccg aagctgctgc
    20941 cgcgttcgca taaaatcagt ttcccgttgt cggcttcgtg gaatttttcc acaatgtttt
    21001 tcatttgaga ggggctgagg aactgaggtt ttttgatgtt gacgacgttg ccggtttttg
    21061 ccatggcaac cactaaatcg gtctgccgcg caaggaatgc gggaagctgg atgacatcgc
    21121 acacttcggc gacgggttgg cactgatagg gttcgtgtac gtcggtaatg acggggatgc
    21181 cgaattccgc tttgactttt tcaaaaatct ttaagccttc ttccaagcct acgccgcgat
    21241 aagaatggat ggaggaacgg tttgccttgt cgaaagatgc tttaaagata tagggaatac
    21301 cgagttttcg ggtaacttcg acgtaatgcg tacaggtttg gagggtggaa tccaaatctt
    21361 ctaaaacgtt gatgccgccg aacagtacga aaggagcatc gttaccgacg gtaatgccgt
    21421 taattttaat atccatgatc aatgtccgtt ttcagggtta cagcacggtg tgcggcggct
    21481 cgtaggagat gatgtcgcgg attttgttgt ttccgtctac caagacgact ttgggttcat
    21541 gcgcggcgat ttcgggttcg gagagttgga cgtaagacat gatgatgacg atatcgcctt
    21601 tctgcaccag ccttgccgca gcaccgttca gacaaatcac gccgctgccg cgtttacctg
    21661 aaatggtata ggtttcaaaa cgttcgccgt tgttgttgtt gacgatggcg actttttcgt
    21721 tggggtagat gcctgccgcg tctaacagat cttgatcgac ggtaatgctg ccgacgtagt
    21781 tcaaatcggc ttcggttacg gtggcgcggt ggatttttcc gccaagcata gtacggaaca
    21841 tgtatttcct tttggtttgt gtgcggtctg acgggcgcga tgccgtctgg aaatctgaat
    21901 gccgtattat agtggagttt ccttcaaatt cataatggcg gctttcggac aaggaaaacg
    21961 gtgtttcaga cggcattgcg gtgtcggctt tatttgggca ggcatctgtt ttgcaaggtt
    22021 tttaaaacgt tttttacggt gttgattctg acctcgatat tttccatctc tgcggtaaac
    22081 cgcagcttat cggcgccggc aaggcggtat tttttgtcgt tctgaatcag caggatgatt
    22141 tcggttggat cgacattatt gtttttaccg aaggttaccg ttaccgcttc gccggccgca
    22201 tcaatggcat cgatacccaa ttcttttgcc ataagccgca ggtggtggct ttcgataagg
    22261 gttttgacgg gttgttcggg caggccgaag cggtcgacga gttcttcgtg tatggcgttg
    22321 atttgctgca cggtttcgca gacggcgagg cgtttgtaga ggacgagacg ctcgtggatg
    22381 tcggggcagt agctttcggg cagtagggcg gggctgtgca gtttgatttc ggtggtgatg
    22441 cccagcggcg cgtcgaggtc gggttggcgg ccttttttga gatcgcgcac ggcttgtttg
    22501 agcatttcgg tgtagagcgt gaagccgacc tgtatcattt cgccggactg cccttcgccg
    22561 aggatttcgc ctgcgccgcg gatttccaaa tcctgcatgg cgagggtgaa acctgcgccg
    22621 agttcgtctg ctgccgcgat ggcgtcgagg cgtttttctg cgtctttggt gatgtattcg
    22681 ggcgtgagca ggtaggcgta ggcttggtgg tggctgcgtc cgacgcgccc gcgaagctgg
    22741 tgcagttgcg ccagtccgaa tttgtcggcg cggttgatga tgatggtgtt ggcgttggga
    22801 atgtcgatac cggtttcgat gatggtggaa cagagcaaca cgttgaatcg ttgctgtaaa
    22861 aagtcgcgca tgacttgttc cagctcgcgc tcgcgcagtt gtccgtgcgc cacgccgatg
    22921 cgggcttcgg gcagcagggt ttccagccgc tcgcgcatat tttcaatcgt atctacttca
    22981 ttgtgcagga aaaatacctg tcctccgcgt ttgagttcgc gcaacacggc ttcgcgcacg
    23041 ctgccttcgc taaagggttt gacaaaggtt ttgacggcga ggcggcggct gggcgcggtg
    23101 gtaatcagcg agaagtcgcg cagtccttcc aacgccatac ttaaagtacg cggaatcggc
    23161 gtggcggtca tggtgaggat gtcgacattg gcacgcaggc gtttgagctg ctctttttgg
    23221 cgcacaccga agcggtgttc ttcgtcgata atcactaaac ctaagttttt gaatttgatg
    23281 tcgtcctgca ccagtttgtg cgtgccgata acgatgtcga ccgtgccgtc cgccatgcct
    23341 tccagcgcgg ctttagtggc tttgctgttg ttgaaacgcg aaaggctagc gactttcaca
    23401 gggaaatcgg cgaaacggtc ggcaaagttt tgcgcgtgct gctcgaccag aagcgtggtc
    23461 ggggcgagta cggcgacctg tttgccgccc atcaccgcca caaacgcggc gcgcagggca
    23521 acttcggttt tgccgaagcc gacatcgccg cacacaaggc gatccatcgg cttcgcttgc
    23581 gtcaaatctt taatcacggc ggcgatggcg gcggcctggt cttcggtttc ctcgtagccg
    23641 aagccatcag caaacgcctg atagtccgac tcgttgattt caaacttgtg tcccgattgg
    23701 gcagcgcgtc gggcgtaaag gttaagcaac tcggcggcgg tatcgcgcgc cttttcggcg
    23761 gctttgcgct tcgccttgtt ccacgcgcca ctgccgagct tgtgcagggc aatgttttca
    23821 tgcgcctgac cggagtagcg gctgattaaa tgcagttgcg aaacgggcac ataaagctgc
    23881 gcttcgcctg cgtattcgag caacatcatt tcgttggttt cgccgcccaa atccattgtg
    23941 atcaagccca tataccgccc gatgccgtgt tcttcgtgca cgacgggatc gccgatattg
    24001 atttcggcaa ggtcgcgcaa caggccgtct gaaacggcgg cgtgtttctt gcggcggttg
    24061 tgggcgcgcg aacgggcgac gtattggtag agatcggatt cggtgatgac ggcgatggca
//
LOCUS       DAWHXS010000019        23050 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWHXS010000019 DAWHXS010000000
VERSION     DAWHXS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.019"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            184..540
                     /locus_tag="WIE22_000580"
     CDS             184..540
                     /locus_tag="WIE22_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ4126717.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGVYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            531..1013
                     /locus_tag="WIE22_000581"
     CDS             531..1013
                     /locus_tag="WIE22_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ4126718.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1026..1619
                     /locus_tag="WIE22_000582"
     CDS             1026..1619
                     /locus_tag="WIE22_000582"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ4126719.1"
                     /translation="MASIQNLYETVIGVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEDLHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1609..2865
                     /gene="nuoD"
                     /locus_tag="WIE22_000583"
     CDS             1609..2865
                     /gene="nuoD"
                     /locus_tag="WIE22_000583"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ4126720.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2865..3338
                     /gene="nuoE"
                     /locus_tag="WIE22_000584"
     CDS             2865..3338
                     /gene="nuoE"
                     /locus_tag="WIE22_000584"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ4126721.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3728..5029
                     /gene="nuoF"
                     /locus_tag="WIE22_000585"
     CDS             3728..5029
                     /gene="nuoF"
                     /locus_tag="WIE22_000585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ4126722.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5029..5466
                     /locus_tag="WIE22_000586"
     CDS             5029..5466
                     /locus_tag="WIE22_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126723.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5756..8017
                     /locus_tag="WIE22_000587"
     CDS             5756..8017
                     /locus_tag="WIE22_000587"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ4126724.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFSYEGLYHESRLKK
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8020..9096
                     /gene="nuoH"
                     /locus_tag="WIE22_000588"
     CDS             8020..9096
                     /gene="nuoH"
                     /locus_tag="WIE22_000588"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ4126725.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            9177..9656
                     /gene="nuoI"
                     /locus_tag="WIE22_000589"
     CDS             9177..9656
                     /gene="nuoI"
                     /locus_tag="WIE22_000589"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ4126726.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9668..10339
                     /locus_tag="WIE22_000590"
     CDS             9668..10339
                     /locus_tag="WIE22_000590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ4126727.1"
                     /translation="MTFSAILFYILATIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            10336..10641
                     /gene="nuoK"
                     /locus_tag="WIE22_000591"
     CDS             10336..10641
                     /gene="nuoK"
                     /locus_tag="WIE22_000591"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ4126728.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLNKLKG"
     gene            10936..11808
                     /locus_tag="WIE22_000592"
     CDS             10936..11808
                     /locus_tag="WIE22_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEZ4126729.1"
                     /translation="MSFSIALPLVYLFIGILIGKTPFRKIKGTFSVILSRWIIPFVIV
                     YNITTYRDGMQYVMLWMIISMGLIILIGKRVDKDPVWNLCLCYLNIGWLGLPIVATLF
                     GDEAAQVLISAYVGSSLFGNSVGVGLLLNNSNFISGLKKTLKSPPVVALVIGMFGIPL
                     GHFVADYFGEVYFFSKIAMGILGMVILGIWLAEIKLNLNDLKKSIKPFLIKNILFIGI
                     VFLFIQIAAYFDLKLILENQKTLYLIPLLPPAANIIVLETAYLKTGRSASMIAYGTIF
                     SLIAISIYILLVQI"
     gene            11809..13833
                     /gene="nuoL"
                     /locus_tag="WIE22_000593"
     CDS             11809..13833
                     /gene="nuoL"
                     /locus_tag="WIE22_000593"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ4126730.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQTATIQ
                     LFPSVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYLAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFEKKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            14017..15180
                     /locus_tag="WIE22_000594"
     CDS             14017..15180
                     /locus_tag="WIE22_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ4126731.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVSQSLMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKSLIKQ
                     CAAGKIDNDEFYVEFNKLWSWKSENKGEGVGNRFQDQTWKRTGKSSDFRKLCIAIQSV
                     FDAPLAERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEKNK
                     FKSAKGASEGSRYIDLSMKLRALLAMQSEIKNLAELDVLVQAEYRDSTDIDWE"
     gene            15290..16786
                     /locus_tag="WIE22_000595"
     CDS             15290..16786
                     /locus_tag="WIE22_000595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ4126732.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGEIRNPHVADM
                     KDINCREFAVLAVLAAAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            16796..18241
                     /gene="nuoN"
                     /locus_tag="WIE22_000596"
     CDS             16796..18241
                     /gene="nuoN"
                     /locus_tag="WIE22_000596"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ4126733.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVG
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARLAAGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            18353..18652
                     /locus_tag="WIE22_000597"
     CDS             18353..18652
                     /locus_tag="WIE22_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ4126734.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(18761..19657)
                     /locus_tag="WIE22_000598"
     CDS             complement(18761..19657)
                     /locus_tag="WIE22_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ4126735.1"
                     /translation="MNPTNDLKAWQLRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(19641..19865)
                     /locus_tag="WIE22_000599"
     CDS             complement(19641..19865)
                     /locus_tag="WIE22_000599"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ4126736.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(20112..21035)
                     /gene="rsgA"
                     /locus_tag="WIE22_000600"
     CDS             complement(20112..21035)
                     /gene="rsgA"
                     /locus_tag="WIE22_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ4126737.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(21040..22896)
                     /locus_tag="WIE22_000601"
     CDS             complement(21040..22896)
                     /locus_tag="WIE22_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4126738.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
BASE COUNT         5225 a         5673 c         6218 g         5934 t
ORIGIN      
        1 cacgaattac ggtgtaaaaa tttatatgac cttataaaat caaataagaa tcgttatcat
       61 aacatgattg tatttattgg gtttttttgg gcgttttgcc gatatttacc ttttaatggt
      121 ttttgaaatt cgctaaaata cgaaattatt gtagaaattt tgttaacgga tttgggtgta
      181 accatgttgt ccgcttactt tcccgtcttt gtctttatcc ttgtcggtct tgcagccggc
      241 gtactgttta tcctgctcgg cacaattttg ggcccgaaac gtcactatgc cgaaaaagac
      301 gcgccttacg aatgcggttt tgaagccttt gaaaacgcaa ggatgaagtt cgacgtgcgc
      361 tattacctcg tcgccatcct cttcatcctg tttgatttgg aggtcgcgtt tatgctgccg
      421 tgggctgtcg tgttcaaaga tttgggcgtg tacggcttct ggtctatgct ggtgtttatc
      481 gtcgttctga cggtaggctt tgtttacgaa tggaaaaaag gtgcgctgga atgggaatag
      541 aaggcgtttt gaaaaaaggt ttcatcacca ccagcgcgga tacggtgctg aactatatgc
      601 gtaccggttc attgtggccg gttactttcg gcttggcctg ctgcgccgtg gaaatgatgc
      661 atgcaggtat ggcgcgttat gaccttgacc gtttcggcat cattttccgt ccgtccccac
      721 gtcaggccga cctgatgatt gtggcaggta cgctgaccaa caaaatggcg cccgccctgc
      781 gccgcgtata cgaccagctc gccgagccgc gttgggtatt gtctatgggc tcgtgtgcca
      841 acggcggcgg ttattatcac tattcttatt ccgttgtgcg cggtgccgac cgcgtcgtgc
      901 cggtagacgt ttatgtgccg ggttgtccgc cgactgcgga agccctgatt tacggcctga
      961 ttcagctcca acaaaaaatc aagcgcactt ccaccatcgc gcgtgacgag taaggagagg
     1021 acgatatggc aagcattcaa aatttatacg agaccgtcat cggtgtgctg ggcaatcagg
     1081 caggcaaagt catttcagct ttgggcgaga ttaccgtcga gtgtctgccc gaacactata
     1141 tctcagtcat gaccgcattg cgcgaccatg aagatttgca tttcgagctt ctggtcgatt
     1201 tgtgcggtgt cgattacagc acttacaaaa acgaagtatg gcagggcaaa cgctttgccg
     1261 tcgtcagcca gctgctttcc gttaaaaaca atcaacgcat ccgcgtacgc gtctgggttt
     1321 cagacgacga cttccccgta gtcgaatccg tagtcgatat ttacaacagc gcggattggt
     1381 acgaacgcga agccttcgat atgtacggca tcatgttcaa caaccacccg gacttgcgcc
     1441 gcatcctgac cgattacggc tttgtcggac atccgttccg caaagacttc ccgatttccg
     1501 gctatgtgga aatgcgttac gacgaagagc aaaaacgcgt gatttaccaa cctgttacca
     1561 ttgagccgcg cgagatcacg ccgcgtatcg tccgtgagga gaactacggt ggccaataaa
     1621 ttaagaaact acaccatcaa cttcggcccg caacaccctg cggcgcacgg cgtattgcgt
     1681 atgattttgg agttggaggg cgaaaccatc gtccgtgccg atccgcatat cggcctcctg
     1741 caccgcggta ctgaaaaact ggctgaaacc aaaacctatc tgcaagccct gccctatatg
     1801 gaccgcttgg actacgtttc catgatggtc aatgagcagg cgtattgttt ggcagtagaa
     1861 aaacttgccg gtatcgatgt acctatccgc gcccagtata tccgtgtgat gtttgccgaa
     1921 gtaacgcgca tcctcaatca cttgatgggc atcggttcgc atgccttcga catcggcgcg
     1981 atgaccgcca tcctctacgc cttccgcgac cgcgaagagc tgatggactt gtacgaagcc
     2041 gtttccggcg cgcgtatgca cgcagcctac ttccgtcccg gcggcgttta ccgcgacctg
     2101 cccgacttca tgcctaaata cgagagcagc aaattccgca atgccaaagt attgaagcag
     2161 ctcaacgaat cccgcgaagg caccatgctc gactttatcg atgccttctg cgaacgcttc
     2221 cccaaaaata tcgacacact cgaaaccctc ctgaccgaca accgcatctg gaaacagcgt
     2281 accgtcggca tcggcgtcgt ctcccccgaa cgcgccatgc aaaaaggctt taccggcgtg
     2341 atgttgcgcg gttcgggcgt ggaatgggac gtgcgtaaga cacagcctta cgaagtgtac
     2401 gacaaaatgg atttcgacat ccccgtcggc gtcaacggcg actgttacga ccgttacctc
     2461 tgccgtatgg aagaaatgcg tcaatccgta cgcatcatca aacaatgttc cgagtggttg
     2521 cgtgtcaatc cgggtccggt cattaccaca aaccacaaat ttgctccgcc caaacgtacc
     2581 gaaatgaaaa caggtatgga agacctgatt caccatttca aactctttac cgagggtatg
     2641 cacgttcccg agggtgagac ctacaccgct gtcgaacatc cgaaaggcga gttcggcgtt
     2701 tacatcattt cagacggcgc aaacaaaccc taccgcctga aaatccgcgc acccggcttc
     2761 gcccatctgc aaggcatgga cgaaatggca aaaggccaca tgctcgccga cgtcgttgcc
     2821 atcatcggta cgcaggacat cgtattcggg gaggttgacc gataatgtta tccgcagagt
     2881 ccttaaaaca aatcgacaca gaactggcga aatatcccgc cgaccaacgc cgttccgcca
     2941 tcatgggtgc gttgcgtatc gcccaaaccg aaaaaggctg gctcgccccc gaaaccatcg
     3001 cttttgttgc cgactatatc ggcatcacgc ctgcacaagc ctacgaagtc gccactttct
     3061 acaatatgta cgaccttgag cctgtcggca aatacaaact gaccgtttgt accaacctgc
     3121 cctgcgccct gcgcggcggt atggctaccg gcgaatacct caaacaaaaa ctcggtatcg
     3181 gctacggcga aactacccct gacggcaagt ttacccttgt cgaaggcgaa tgcatgggcg
     3241 catgcggcga cgctcccgtt atgctggtca acaaccacag catgtgcagc tttatgaccg
     3301 aagaagcgat tgagaagaaa ctggcggagt tggagtaggt cgtctgaaac gacgatttaa
     3361 acgtaggtcg gatacttgta gccgacagag tgggtaaaaa ggcaaaatgt cggatttaag
     3421 aatccgccct actgaaatac cgaaatgccg tcattcccgc gcaggcggga atccaccggt
     3481 aagattcggt ttctgaattt aataagacat tgcttaccat tgaggatgga ttcccgcctg
     3541 cgcgggaatg acgacagaca agcaagtggt cgagatccaa caaaaacgat taaaggtcgt
     3601 ctgaaaatat cgatttgata aactagattt tatttcagac gacgttacaa gccggtacac
     3661 aaagacatct taaggtcgtc tgaaacagcg gccgcaaccg atacgaaaac aaacaggcac
     3721 accaaaaatg gctatttacc aatcaggcgt gatttttgac caagtggata ccgccaatcc
     3781 cgattgctgg acattggacg aatacgtcaa acgtggcggt tataccgccc tgcgtaaaat
     3841 cctgtccgaa aacatctcgc aaaccgatgt gattgacgaa gtcaaaacct ccggcttgcg
     3901 cggtcgtggc ggtgcgggct tcccgaccgg tttgaaatgg agctttatgc cccgttcttt
     3961 ccccggcgaa aagtacgtcg tttgcaacac cgacgaaggc gagccgggta catttaaaga
     4021 ccgcgacatt attatgttca acccccatgc cctgattgaa ggcatgatta tcgccggtta
     4081 cgcgatgggc gcgaaagccg gttacaacta tatccacggc gaaatttttg aaggctacca
     4141 acgctttgag gccgctttgg agcaggcgcg tgccgcaggc tttttgggta aaaatatttt
     4201 gggttcggat tttgaattcg aactcttcgc ccaccacggc tacggtgcat acatctgcgg
     4261 cgaagaaacc gcattgcttg aatcgctgga aggcaaaaaa ggccagccgc gctttaaacc
     4321 gccattccct gcttcgttcg gcctgtacgg caaaccgact accatcaaca ataccgaaac
     4381 tttctcctcc gtttcattca ttatccgtga cggcggacag gcatttgccg ataaaggtat
     4441 tccgaatgca ggcggtacca aattattctg tatttccggc catgtcgagc gtccgggcaa
     4501 ctatgaagtg ccattgggca caccgtttgc cgaagtcttg aaaatggcag gcggtatgcg
     4561 cggcggcaaa aaactcaagg ccgttattcc tggcggttcg tccgcgcccg tattgcctgc
     4621 cgacatcatg atgcagacaa atatggacta cgactcgatt tccaaagcag gctcgatgct
     4681 gggttctggc gcgattatcg tcatggacga agacgtgtgc atggtgaaag cccttgagcg
     4741 cctgagctac ttctactacg acgagtcttg cggccaatgt acgccttgcc gagaaggcac
     4801 cggctggctt taccgcatcg tccaccgcat cgtagaaggc aaaggccgca tggaagattt
     4861 ggatttgctg gattctgtcg gcaaccaaat ggcaggccgc accatctgcg cccttgccga
     4921 tgccgccgtc ttccccgtcc gaagctttac caagcatttc cgtgatgagt ttgtgcatta
     4981 catcgaacac ggcggaccga tgaaagagca taagtgggga gggtggtaat ggtggaagct
     5041 aaaattttta ttctatacgg tgcagccaac aaaggtaaga gtacgacact caatacgctt
     5101 tttaatcaga tttgttggaa attttctaaa tttctagtct tttttgaaag acatggaaat
     5161 ggcttagatt ttgttgcagt atttgatcat gaaggtcaga gaattggttt ttattcatct
     5221 ggtgataatg aatacgaggt taggagaaat ttatacaaac tttattcgca taattgtgat
     5281 tttatttttg gcacgtcaag gacacggggt ggtagttgcg atgcagtagg atgttatgca
     5341 gagttattgc atggcgatgt aaatataatt aattggtgtg aaaagtttga gcctacagat
     5401 gaagacaatg agcgtgctgt taaagagtta tttaagtcat ttaaaaatat aataaatgag
     5461 ttatagtttt agttggtttt atattggtta aaagcaaaat gctaaaaatt taactttgcc
     5521 gtcattcccg cgtaggcggg aatccatagt ggaatttaca gaacccgata tttgaaaagc
     5581 agttgccgaa attcaaaaaa tggattcccg cctgtgcggg aatgacggca aggtggcagt
     5641 aaacgtttta aacagtattg attgtcaatg aaactcaaaa ggccgtctga aacccatttt
     5701 tcagacgacc tccataaaag attatttatc aaatacccgt aactaggaac gaaccatgtt
     5761 acaaatcgaa atcgacggca aacaagtatc tgtggagcag ggcgcgacgg tgattgaagc
     5821 cgcgcacaag ctcggtactt atattccgca tttctgttac cacaaaaaac tttccatcgc
     5881 cgccaactgc cgtatgtgtc tggtgaacgt agaaaaagcc ccaaaacccc tgcctgcctg
     5941 tgccacgccg gttacagacg gcatgattgt gcgtacgcat tcggcaaaag cccgagaggc
     6001 gcaggaaggc gtgatggagt tcctgctcat caaccatccg cttgattgtc cgacctgcga
     6061 ccaaggcggg gaatgccagt tgcaggattt ggcggtgggc tacggaaaaa ccaccagccg
     6121 ctacaccgaa gaaaaacgtt ccgtcgtcgg caaagatatg gggtccttgg tttccgccga
     6181 ggaaatgagc cgctgtatcc actgcacccg ctgcgttcgt ttcactgaag aaatcgccgg
     6241 tttgcaggaa attgcgatgg tgaatcgcgg cgaacactcc gaaatcatgc cctttatcgg
     6301 caaaacggtg gaaaccgaat tgtcgggcaa cgtcattgat ttgtgtcccg tcggcgcgct
     6361 gaccagcaaa ccgttccgct tcaatgcgcg tacttgggaa ttgaaccgcc gcaaatccgt
     6421 ttccgcccac gacgctttgg gcagcaacct gattgtacaa accaaagacc ataccgtccg
     6481 ccgcgtgttg ccgctggaaa atgaagcgat taacgaatgt tggctgtctg accgcgaccg
     6541 tttttcctac gaaggcctgt atcacgaaag ccgtctgaaa aaaccgaaaa tcaaacaggg
     6601 cggcgagtgg atggacgtgg attggaaaac cgcgttggaa tatgtccgca gcgcgattga
     6661 atgtatcgcc aaagacggca accaaaacca agtcggcatt tgggcgaacc cgatgaatac
     6721 ggttgaagag ctgtatctgg ccaaaaaact tgccgacggc ttgggtgtta aaaactttgc
     6781 cacccgtttg cgccaacaag acaaacgtct ttcagacggc cttaaaggtg cgcaatggtt
     6841 gggacaaagc attgaatcgc tggcggacaa cgatgccgta ttggtagtcg gtgcgaacct
     6901 gcgcaaagaa cagccgctcc tgaccgcccg cctgcgccgc gccgccaaag accgtatggc
     6961 gttgagtgta ttggccggca gtaaagaaga gttgttgatg ccgcttctct ctcaagaagc
     7021 cgcacatccc gacgagtggg caggccgtct gaaaaacctg tctgccaatg cggaacacgc
     7081 cgttaccgcc agcctgaaaa acgctgaaaa agcagcggtg attttgggcg cggaagtgca
     7141 aaaccatcct gattacgccg ccatttatgc cgccgcgcaa gagctggcgg atgcaaccgg
     7201 cgcggtactg ggtattttgc cgcaagccgc caacagcgtc ggtgcggacg tattgggcgt
     7261 gaactcaggt gagagcgtta ccgaaatggc aaacgcgccg aaacaggcag tcttgctgct
     7321 caacgtcgag cctgaaattg atacggttga cggtgcaaaa gccgtagccg cgttgaaaca
     7381 ggcaaaaagc gtgatggcgt ttacaccgtt tgtcagcgaa acgctgctgg acgtgtgcga
     7441 cgtgttgttg ccgattgcgc cgtttaccga aacctcaggc agcttcatca atatggaagg
     7501 ccgtctgcaa tctttccacg gcgtggtaca aggcttcggc gattcgcgtc cgatgtggaa
     7561 agtgttgcgc gtattgggca atctgtttga cctgaaaggt tttgaatacc acgataccgc
     7621 tgcgattttg aaagacgcgc tggatgcaga aagcctgtcg tccaaactgg acaaccgcag
     7681 tacatggaca ggggagggcg ttcagacggc atcagaccgc ctcgtccgtg tcggcggcgt
     7741 cggtatttat cacaccgatg ccatcgtacg ccgttccgca ccgttgcaag aaaccagcca
     7801 tgccgccgtg cctgccgcgc gtgtaaatcc gaacacattg gcacgcttgg gcctgcaaga
     7861 cggacaaacc gctgtcgcca aacaaaacgg cgcaagcgta tcggttgctg tcaaagccga
     7921 tgccggtttg cctgaaaacg tggtgcatct gccgctgcat accgaaaatg ccgcgctggg
     7981 tgcgttgatg gacactattg aactggcggg agcttgatca tgcaagaatg gttccaaaac
     8041 ctctttgccg caacgctcgg tctgggcgat ttgggcatca ccgtaggctt ggtggtatcc
     8101 gtcatcgtca aaatcgtgat tatcctgatt ccgctgattc tgaccgttgc ctacctgacg
     8161 tatttcgaac gtaaagtcat cggcttcatg cagcttcgcg tcggtccgaa cgtaaccggc
     8221 ccgtggggtc tgattcagcc gtttgccgac gtgttcaaac tcttgtttaa agaggtaacc
     8281 cgtccgaagc tgtcaaacaa agccctgttc tacatcggcc cgattatgtc gcttgctccg
     8341 tctttcgcag catgggcagt gattccgttc aacgaagaat gggtgctgac caacatcaat
     8401 atcggccttt tgtacatcct gatgattacc tcgctgtcgg tttacggcgt gatcatcgcg
     8461 ggctgggctt ccaactccaa atattcgttc ttgggtgcaa tgcgtgcttc cgcacaaagc
     8521 atttcctacg agattgccat gagtgccgcg ctggtgtgcg ttgtgatggt atcgggcagc
     8581 atgaacttct ccgacatcgt tgccgcacaa gccaaaggca tcgcgggcgg ttccgtgttc
     8641 tcgtggaact ggctgccgct cttccccatc ttcatcgttt atctgatttc cgccgttgcc
     8701 gaaaccaacc gtgcgccgtt tgacgtggca gagggtgagt ctgaaatcgt tgccggtcac
     8761 cacgtcgaat attccggctt cgcgttcgcg ctgttcttcc ttgccgaata cattttcatg
     8821 attctgattg ccgcgctgac atcgctgatg ttcctcggcg gctggttgtc cccgttcccg
     8881 caaagttggg gctttatcgg cacaccttcc gcattctgga tgttcgtgaa aatggcggcg
     8941 gtgttgtact ggtacctgtg gatccgtgca accttcccgc gctaccgtta cgaccaaatc
     9001 atgcgtttgg gctggaaagt gctgattccg atcggcttcg cctacatcgt ggttttgggc
     9061 gtgtggatga tttcaccgct gaatttgtgg aaataagttt cagacggcat cttgaggccg
     9121 tctgaacaaa gcgattttga atacctaacg aaatccctgt tttgagggaa cataatatgg
     9181 ctaacttagt aaaaaccttt ctgcttggcg aattggtaaa aggtatgggc gtaacgctca
     9241 aaaacttttt cgcccgcaaa gacacgattt attttcccga agagaaaacg ccgcaatccg
     9301 tgcgtttccg cggcctgcac gcgcagcggc ggtatccgaa cggtgaggaa cgctgcattg
     9361 cgtgtaagtt gtgcgaggcg gtgtgtccgg caatggcgat caatatcgaa tcggaagaac
     9421 gcgaagacgg cacccgccgc accaagcgtt acgacatcga cctgaccaag tgcatcttct
     9481 gcggcttctg cgaagaggcc tgcccaaccg atgcgattgt ggaaacgcat atttttgaat
     9541 accacggcga gaaaaaaggc gacttgcaca tgaccaagcc gatcctcttg gcaatcggcg
     9601 acaaatacga agccgaaatc gccaaacgca aagccgctga cgcgccgtac cgttaaggag
     9661 cagacgaatg actttttccg cgattctgtt ctatatcctt gccaccatcg tcttatacgg
     9721 cgcgctgcgt accgttaccg ctaaaaaccc tgtccacgcc gctttgcatc tggtgctgac
     9781 cttctgcgtg agcgcgatgc tttggatgct gatgcaggct gagtttttgg gcgtgacgct
     9841 ggtggtggtt tacgtcggcg ccgtgatggt gttgttcctg ttcgtcgtga tgatgttgaa
     9901 catcgacatt gaagaaatgc gtgccggttt ctggcggcac gcgcctgttg ccggtgtggt
     9961 cggcacattg ttggcggttg cgctgatcct gattctggtc aacccgaaaa ccgaccttgc
    10021 cgcatttggt ctgatgaaag acattcctgc cgattacaac aatatccgcg atttgggcag
    10081 ccgtatttat accgactatc tgttgccgtt tgaattggcg gcggtattgc tgttgttggg
    10141 tatggtggcg gcgattgcgc tggttcaccg taaaaccatc aatccgaaac gtatggatcc
    10201 tgccgaccag gttaaagtac gcgccgacca aggccgtatg cgtctggtga aaatggaagc
    10261 ggtcaaaccg caagtcgaat ctgccgaaga aagcgaagtt tcagacggtc tcaagccgga
    10321 aggggagggc aaagcatgat taccttgacg cattatctgg tattgggcgc gctcctgttc
    10381 ggtatcagcg cgatgggcat ctttatgaac cgcaaaaacg tgctggtctt gctgatgtcc
    10441 atcgagctga tgcttttggc ggtgaacttc aactttatcg ccttctcgca acatttgggc
    10501 gataccgccg gacagatttt cgtattcttc gtactgaccg ttgccgctgc cgaatctgcc
    10561 atcggtttgg cgattatggt attggtgtac cgcaaccgac aaacaatcaa cgttgccgat
    10621 ttgaacaaac ttaaaggttg atttggaatt tttaaagaaa aaaggagata gataatgttt
    10681 gatattgctg ctgttggatt aggtgcagca ggagttagct taatcaaaca gttacaggta
    10741 aaaactcttg tttgccatat caaggcactt tggtctccac acgcccatat atgcataaat
    10801 tgtggattag tatgagtgaa agagaatgtt tacaatttca agggctcatt ggcagccatt
    10861 gggctgtatg gcgggtaatt gggtaaaaca atgggttttt gaaaatttta gaatttcaga
    10921 aagtggagag tgattatgtc attttcaatt gctcttccat tggtatatct gtttattggc
    10981 attttgattg ggaaaacccc ttttagaaaa attaaaggca ctttttctgt aatattgagc
    11041 agatggatta tcccttttgt tattgtctat aacataacga catatcgtga tggtatgcag
    11101 tatgttatgt tatggatgat tatttcgatg gggttgatta tcctaatcgg gaaaagagtt
    11161 gataaagatc ctgtttggaa tttgtgtttg tgctatttga acatcggatg gttggggctt
    11221 cccatagttg caaccttgtt tggagatgag gcggcgcaag tgcttatttc ggcatacgtg
    11281 ggaagttctt tattcggaaa ttcggtcggg gtcgggttgt tattgaataa tagtaatttc
    11341 atatccggat tgaaaaagac tttaaaatcc cctcccgttg ttgcattggt aataggaatg
    11401 ttcggtattc cgctagggca ttttgtagca gattattttg gggaagttta ttttttctcg
    11461 aaaatagcaa tgggaatatt ggggatggtg atactcggta tttggctggc tgagataaaa
    11521 ctaaatttga atgatttgaa gaaaagtatt aaaccgtttc taataaaaaa cattttattt
    11581 atagggattg tttttctttt tatccaaata gctgcatatt ttgacttgaa gttgattctt
    11641 gaaaaccaaa aaacattgta tttaataccg ttgctgccgc ctgctgccaa tattattgtg
    11701 ttggaaacgg catatttgaa aacagggcgt tcggcttcta tgattgcgta tggtacgatt
    11761 tttagtttaa tcgcaatcag catttatatt ttactggtgc aaatttaaat gaacgacatg
    11821 actttatatc tcatcatcgc cctcgtgcct ttggcaggct cgctgattgc gggtttgttc
    11881 ggcaacaaaa tcggccgcgc cggcgcgcat acggttacga tactcggcgt ggcggtgtcc
    11941 gccgtgctgt cggcttatgt gttgtggggc tttattgacg gcagccgcgc caagtttgac
    12001 gagaacgtct atacctggct gacaatgggc ggtttggatt tctccgtcgg cttcttggtc
    12061 gatacgatga cggcgatgat gatggtcgtg gtaacgggcg tgtcgttgat ggtgcatatc
    12121 tacaccatcg gctatatgca cgatgaaaaa gtcggctacc aacgcttctt cagctatatt
    12181 tctttgttta ctttcagcat gttgatgctg attatgagca acaacttcat ccagctcttc
    12241 ttcggttggg aagcggtggg cttggtgtcg tatctcttga tcggtttcta tttcaaacgt
    12301 ccgagcgcga catttgccaa cctgaaagcc tttttgatca accgtgtcgg cgacttcggc
    12361 tttttgctcg gtatcggctt ggtgcttgcc tatttcggcg gcagcctgcg ctatcaagat
    12421 gtattcgctt atctgcctaa tgttcagacg gccaccattc aattgttccc cagtgtggaa
    12481 tggtctttga ttactgtaac ctgtttgctc ctgtttgtcg gtgcgatggg taaatcggca
    12541 caattcccgc tgcacgtctg gctgcctgat tcgatggaag gcccgacccc gatttctgca
    12601 ttgattcacg ccgcaaccat ggttaccgcc ggtctgttca tggtatcgcg tatgtcgccg
    12661 atttatgaga tgagcagcac tgcgctgtct gtcatcatgg taatcggtgc gattaccgcc
    12721 ctgtttatgg gtttcttggg cgtgattcaa aacgacatca agcgcgtggt tgcgtattcc
    12781 accctgtcgc aactgggcta tatgaccgtg gctttgggcg cgtccgccta ttccgtggcg
    12841 atgttccatg tgatgaccca cgccttcttt aaagccttgc tgttcttggc ggcaggtagc
    12901 gcgattatcg gtatgcacca cgaccaagat atgcgccata tgggcaacct gaaaaaatat
    12961 atgccggtta cttggctgac tatgctgatc ggcaatttgt cgctgatcgg tacgccgttc
    13021 ttctccggct tctattctaa agattcgatt atcgaagcag cgaaatacag caccctgccg
    13081 ggcagcggct ttgcctattt tgccgtcctc gccagcgttt ttgttactgc gttctacgca
    13141 ttccgccaat actttatggt gttccacggc gaagagaaat ggcgcagctt gcctgaacat
    13201 catgacgatc atcatggcga agaacatcac agcttgggca aaaacgacaa tccgcacgaa
    13261 agcccgttgg ttgttaccct gcctttgatt ttgcttgcca ttccgtccgt catcatcggc
    13321 taccttgcca tcgagcctat gctttacggc gatttcttca aagatgtgat tttcgtcaat
    13381 accgacgcgc atccgaccat gcacatcatg aaggaagagt tccacggcgc attggcaatg
    13441 gtgtcgcaca gcctgcattc gcccgtactc taccttgcta tcgcaggcgt gttgagcgca
    13501 tggcttttgt acgtcaaact gccgcacctg ccagcgaaaa ttgcacagac gttccgtccg
    13561 atttacgttt tgtttgaaaa aaaatactac ctcgacgccc tgtatttcaa cgttttcgcc
    13621 aaaggcacac gcgcattggg taccttcttc tggaaagtcg gcgataccgc cattatcgat
    13681 aacggcatcg tcaacggctc tgccaaactg gtcggcgcga ttgccgcgca agtgcgtaaa
    13741 gcccaaaccg gctttatcta cacctacgcc gccgctatgg tattcggcgt attggtactg
    13801 ctcggcatga ccttctgggg attgttcaga taaaggcgga gtttcagacg acctgttcat
    13861 agttttgtag aggtcgtctg aaaagtttga gaagtgggca ttaagtcaaa gggtggaaca
    13921 aaatggtacc caacaggcgt gaaatagcca acatgcaaat tatctgtttc ctaaattttt
    13981 taggcaatct caacaaatat agaggagtca aaagggatga cacagttatt tttacattca
    14041 gatacaccaa atgagaagga actgatttca atatatgaga aagctgtttc agaggcagta
    14101 gagctgtata ttttctctgc gtatctcact cattgggatg taaaaaataa gctcaatagc
    14161 aaattgcaag agtttaaatt tatttttgga caagatttcg gattgtctaa aaaagaggct
    14221 attcggaaag tgttgaaatg gcttccgtcc catttgaaat tcacactgat ggtagctcaa
    14281 ggcattcagg gttttcatcc taaagctgta ttttggaaga atgataagaa tgaatattat
    14341 gctttaatcg gttcttcaaa tttgactcat gctgcgttta atagcaatta tgaagcaaat
    14401 attttgacta aaatttccga gcaggatttt ataaaagtca aatcttgggc tgatgaaatt
    14461 gcgatgaaat ctatcccagt atcggaagat tggcttgagg agtatcagga agctgaaatt
    14521 aattataaga aatcaactgt tagccaatca ttgatggata agttatttat ggaaatgcca
    14581 aattataatc aagaactgat tgcagcacgg agaaaacaga tgagaaacca tcaaactgtg
    14641 tgtaatcaat taaaaagtct tattaagcaa tgtgctgcag gaaagataga caatgatgag
    14701 ttttatgtgg aatttaataa attatggtct tggaaaagcg aaaataaagg agaaggggta
    14761 ggtaatcgat ttcaagatca aacgtggaaa aggaccggaa agtcttcaga ttttagaaaa
    14821 ttatgcattg ctatccaatc agtttttgat gcaccacttg ctgagagaga taatgttgtt
    14881 gccaagcaaa ttgattggct aaaagaatgt ggagtttcca ctcgtggttc agttttttct
    14941 gaaatgcttt gtcaagaata tccagataga tatccagtgt taaacgctcc tattaaaaaa
    15001 tttttagaaa aaaataaatt taagtctgca aaaggagcaa gtgaaggctc tagatatatt
    15061 gatttaagta tgaagttaag agctttacta gcaatgcagt cagaaataaa aaatttggct
    15121 gaattagatg ttttagtgca ggcagaatat cgcgatagta cagatattga ttgggagtga
    15181 gtaaagaggg gagagagtat tcatcccttt ttcagacgac ctcctgaaac gtcgtctgac
    15241 aaactaaccc gcactacgat atttttattt taaccacagg ttaaccacta tgttttccaa
    15301 ctacctactc agcttggcaa tatggatacc catcgccgca ggtgtgctgg ttttgtcgac
    15361 cggtaaggac agccgtgcgc cgctggcgcg tgtgcttgcc tttatgggtg cgcttgccgg
    15421 tttcttggta acgctgcccc tgtttaccgg tttcgaccgt ttgagcggcg gctatcaatt
    15481 taccgagttc cacgagtgga ttccgctgct gaaaatcaac tatgcattgg gcgtggacgg
    15541 tatttcagtg ctctttatca tcttgaatgc gtttattacg ctgctggtgg tattggccgg
    15601 ttgggaagtc attcagaaac gtccggcgca gtatatggcc gctttcctga tgatgtcggg
    15661 tttgattaac ggcgcgtttg ccgctcagga tgcgattctg ttttatgtgt tctttgaggg
    15721 tatgctgatt ccgctgtacc tgattatcgg tgtatggggc ggcccgcgcc gcgtctatgc
    15781 gtcggtcaag ctgttcctct atacgctgat gggttcactc ttgatgctgg ttgccatggt
    15841 ttacctgtac tatcaaacag gcagcttctc tattgtcgat ttccaaaaca tcaaacagat
    15901 tccgttgggc gtacagcagc ttttgtttgt ggcattcttc ctgtcatttg ccgtaaaagt
    15961 gccgatgttc cccgtacaca cttggctgcc ggatgcccac gttgaagcac cgaccggcgg
    16021 ttcgatggtg ttggctgcga ttacgctgaa actgggtgcg tatggtttct tacgctttat
    16081 cctgccgatt atgccggatg cggcacgcta ttttgccccc gtgatcatcg tattgagtct
    16141 gattgccgtg atttatatcg gtatggtggc tttggtgcaa accgatatga aaaaactggt
    16201 ggcgtattcg tccatcagcc atatgggttt tgtaacgctt gggatgtttt tgtttgttga
    16261 cgggcagttg gacgactggg cattgaaagg cgcggtcatt cagatgattt cgcacggttt
    16321 cgtgtcggcg gcgatgttta tgtgcatcgg tgtgatgtac gaccgcctgc acacgcgcaa
    16381 tatcgccgat tacggcggcg tggtcaacgt gatgcccaag tttgcggcgt ttatgatgtt
    16441 gttcggtatg gcgaacgccg gtttgccggc gacttccggc ttcgtgggcg agtttatggt
    16501 gattatgggc gcggttaaag tgaatttctg ggtgggtgcg ttggcggcta tgacgctgat
    16561 ttacggcgcg tcttataccc tgtggatgta caaacgcgtg attttcggcg agattcgcaa
    16621 tccgcacgtt gccgatatga aagacatcaa ttgccgcgaa tttgcggttt tggcggtttt
    16681 ggcggcggct gttttgggta tgggcctgta tccgaacgcg tttatcgaag tggtacatca
    16741 ggcggcaaac gatttgattg cccatgtggc acaaagcaag atttgaggtg tgtaaatgaa
    16801 ctggtctgat ttgaatttaa tgcccgccat gcccgaaatc gtgctgctgt cgctgctggt
    16861 gttattgttg ctggcagact tgtgggtcag tgatgacaaa cgccgttgga cgcattacgg
    16921 cgcgttggca acggtggcgg ttacggctgt ggtgcagctt gccgtttggg aacagggcag
    16981 cacgtcttcg ttcaacggca tgtatattgc agacggtatg tcgcgtttgg caaaaatggt
    17041 tttatatgcc ttgacctttg ccctgtttgt ctatgccagg ccctacaacc aagtgcgcgg
    17101 tattttcaaa ggcgagtttt acaccctgtc gctgtttgcc ctcttgggta tgggcgtgat
    17161 ggtgagcgcg gggcattttt taactgccta tatcggtttg gaactcttgt cgcttgccct
    17221 ttacgccctg attgccctgc gccgcgattc cggctttgcc gccgaagccg ccttgaaata
    17281 ttttgttcta ggcgcgctgg catccggcct gctgctctac ggtatttcta tggtttacgg
    17341 cgcaaccggt tcgctggaat ttgccggcgt gcttgcctct tccttcaatg aagaagccaa
    17401 cgaatggctg ttgaaactgg gtctggtgtt tatcgtcgtc gccgttgggt tcaaactcgg
    17461 cgcggtaccg ttccatatgt gggtgcccga cgtgtatcac ggcgcgccca cttctgttac
    17521 cgccctagtc ggcactgccc cgaaagtcgc cgccgtcgtt ttcgctttcc gcatcctcgt
    17581 taccgggctg ggaaccgtgc atcatgactg gtctctgatg tttgccctgc ttgccgccgc
    17641 ctcgctgctg gtcggcaacc ttgccgccat catgcagacc aatatcaaac gtatgctcgc
    17701 ctattccacc gtatcgcata tgggtttcat cctgttggcg tttatggcgg gcgcggtcgg
    17761 ctttgcggcg ggtctctatt acgccattac ctacgcgctg atggcggcgg cagggttcgg
    17821 cgtgttgatg gtgttgtcgg atggggacaa cgagtgcgaa aacatcagcg atttggcagg
    17881 gctgaaccaa caccgcgtat ggcttgcctt tttgatgtta ctggttatgt tctctatggc
    17941 gggcattccg ccgctgatgg gtttttacgc caaatttggc gtgattatgg cactcttaaa
    18001 acaaggctat gtttggttgt cggtgtttgc cgtcgtgatg tcgctgattg gcgcgttcta
    18061 ctacctgcgc gtggtcaaag tcatgtattt tgacgaatcc ggccgcgccc gtctcgccgc
    18121 cggcggcaac aatgccgcaa aattcctctt gagcgtcaac gcgctgctgt tggtgctgtg
    18181 gggcattatg ccgcaaaccg ttatcgactg gtgcgccaag gcgttggaaa acacgctgta
    18241 agccgccgca acggcagccg tgtcagaggc tgccgttttt gttaagatat gccgttccgc
    18301 aatgcagttc agacggcatc gccgccgaca acgcccaaac agaaagccca ccatgaccgc
    18361 atccatgtac atccttttgg tcttggcact catctttgcc aacgccccct tcctcacgac
    18421 cagactgttc ggcgtggccg cgctcaagcg caaacatttc ggacaccact tgatcgagct
    18481 ggcggcaggt ttcgcgctga ccgccgttct tgcctacatc ctcgaatccc gtgcgggagc
    18541 ggtacacaat cagggttggg agttctacgc caccgtcgtc tgcctgtacc tgatttttgc
    18601 gtttccctgt ttcgtgtggc ggtatttttg gcacacgcgc aacagggaat aggcaggcat
    18661 aggaatgccg tctgaaaccc tttcagacgg catttgtttc attcaagtgc aggccggcat
    18721 cgctgtgccg gcacgtttca gccggcgata tacgccggtt ttaatatttg cgggcgactg
    18781 caaattctgc caactgccgc aggcgcaggg ctttgtcgcc gaagggttcg agcagcgcga
    18841 ccgcttcggc aaccagtttg tgtgcgtatg agcgcgccgc ttccaagccc atcagtttca
    18901 cataagtcgg cttgtcgttg tctgcgtctt tgcccgccgt tttgcccaaa gtcgccgtgt
    18961 ccgcttcaca atccaacaca tcgtcaatga cttggaacgc cagccccagt tttgccgcat
    19021 aatcgtccaa taccgccaga ctgtcttcag acagattcgg gcaggacatc gcccccaata
    19081 aaaccgccgc acggattagc gcacccgttt tcaggctgtg catctgttcc aaatcggctt
    19141 gaaccatttg tttgccgaca ttcgccaaat cgattgcctg accgcccgcc atgcccgcgc
    19201 tgccgcccgc tttcgccaac accgacaaca ttgccaactg gcgtgctgcg ggcagttctg
    19261 tcggacggct taacacgtcg aaggcttggg tttgcaaagc gtcgccgacc agtagcgcgg
    19321 tggcttcgtc gtattgtata tggcaagtcg gtttgccgcg ccgcaggctg tcgttgtcca
    19381 tcgccggcat atcgtcgtga accaaagaat agacgtggat catttcgatt gccgccattg
    19441 cctgtccgac cgcatccgcc acggctccgc ccaattccga agccgccagc accagcatcg
    19501 gacgcagacg tttgcccccg tccaaagccg cataacgcat ggcttcgtgc agtgtgtgcg
    19561 gtatttcccc ctcagacggt aaaaaccgtt caagcagcag ctctgtttgc gcctgcgccc
    19621 taagctgcca tgctttcaaa tcattcgtcg gattcaaggt tcaactcctt cagcccgtcc
    19681 gcatccaaaa cctgtaattt ctgttcgact tgtgccagtt tggtttggca gtacctgacc
    19741 agctcgttgc cttcctgata ggcggcaagc gcgtcttcca agggcatttc gccctgcata
    19801 gactgcgtca gcgattcgag gcgcgacaag gcttcttcaa acgatttcgg ggcgtttttc
    19861 ttcatcgtat ttccttttcg gttgaaaccc cgccctttag ggcggcagga tcagacttta
    19921 tttgggaggg gtgtaaccct ttccaaatca gggcaataca tagggcggtg ctttatgtgc
    19981 cgtcctgtgt gttggaacat agtttcggat gttccggtaa aaagcggatt gtagcatttt
    20041 tgaaaaacgg atgccgtctg aaacccgaat ccggcttcag acggcatttt ttccgcccag
    20101 gcggcaaggc gttacccggg cagttcgtcg gtgatgccct gcaaaaaggc gaggcgttcg
    20161 gggcttgccg ccccggtttg cgcggcggct ttgaaggcgc agccgggttc ggcgcggtgg
    20221 gtgcagttgt ggaagcggca ttgcccgaca aggtggcgga aatcggggaa atagcgcggc
    20281 aaatcggcgg cttggaggtg gtgtaaacca aattcttgca aacccgggga gtcgatgagt
    20341 tgggtttcgc cgttcaaatc ataaagccgg gcgtgggtgg tggtgtgttt tcccgagtcg
    20401 agtgcggcgg aaatgtcgcc ggtgcgggcg gtttggctgc ccaaaagggc gttggtcagg
    20461 gtggatttgc ccataccgct ctgcccgagc aggatgttgc tgtgcccttg cagggcgggg
    20521 cgcaggctgc cggcgttttc cagtgcgcgg gtttcgatga cgggataacc cagcgtttcg
    20581 tagaatttga gtttttcgcg ccaaagggcg gtttcgggca ggtcggcttt gttcaggacg
    20641 atgacggctt caataccggc ggcttcggcg gcaagcaggg cgcgttgcag cagccgcacg
    20701 ctcggactcg ggacggcggc ggttacgatg aggagttggg taacgttggc ggcgatgagt
    20761 ttggttttcc acgcgtcttg gcggtagagc aggctttggc gcggtaaaaa atcttcaatc
    20821 acaacttgtt cggcgttgac ggggctgatg cggacacggt cgccgcaggc gaaatcgacg
    20881 cgttttttgc gggtgctggc ttcgtaggtt gtgccgtcgg gcgtgcgcac gatgtagcgg
    20941 cggccgtagc tggcggtaat ttgggcggtg tcgttcatgg tttctttggg gttgggtgtg
    21001 gaaatgccgt ctgaaaacgg gtgttcggac ggcatcggtt cagtcgtgct gccactcgac
    21061 gtgttcgttg aggaagccgc cgctctggtg cgcccagagt ttggcgtaaa gcccgcgttt
    21121 ttcgaggagt tcggcgtgtg tgccttcttc gatgatgcgg cctttgtcga ggacgacgag
    21181 cctgtccatt gcggcgatgg tggagaggcg gtgggcgatg gcgatgacgg ttttgccgtc
    21241 catcattttg tcgaggcttt cctggatggc ggcttcgact tcggaatcga gcgcgctggt
    21301 ggcttcgtcg agcagcagga tgggcgcgtc tttgagcatc acgcgggcaa tggcgatgcg
    21361 ctggcgttgc ccgccggaga gtttcacgcc gcgttcgccg acgtgcgcgt catagccgcg
    21421 ccgccctttg gcatcggaaa gttcggggat gaagccggcg gcttcggcgc gttcggcggc
    21481 ggaaaccatt tcggcatcgg tcgcgtcggg gcggccgtaa ataatgttgt cgcgcacgga
    21541 acggtgcagc agcgaggtat cttgcgtgac caaaccgatt tgggcgcgta aagattcttg
    21601 ggtaacgccg cttatgtcct gcccgtcgat cgaaaccgtg ccgctttgcg gttcgtagaa
    21661 gcgcaaaagc aggttgacga tggtggattt gcccgcgccg ctgcgtccga tcaagccgac
    21721 tttttcgccc gggcggatgg tgaggttgaa gccgttgagc agcggtttgc cggcttcgta
    21781 gcagaaatcg atgtgttcaa atttgatttc gccttgcgac actttcagcg gcagggcgtt
    21841 cggcttgtcg aggatggtgt gcggtttgga cagggttgcc atgccgtcgc cgacggtgcc
    21901 gatgttttca aacagccgcg cggattccca cataatgtat tgcgacagcc cgttgacgcg
    21961 caacgccatc gcggtggcgg tggcgaccgc gcccacgccg acctgcccgt tgtgccagag
    22021 ccagatgccc agtgcggcgg tggagagggt cagggaggtg ttgacgatga agctgcacga
    22081 atgcagcagc gtcgccagcc gcatttgggc gcgcaccgta accataaatt cttccatcga
    22141 ctgcttggca taggcggctt cgcgcgcgcc gtgggagaag agtttgacgg tggcgatatt
    22201 ggaataggca tcggtaatgc ggccggtcat cagcgagcgg gcatccgcct gccatgcggc
    22261 ggtttgcccc aatttgggaa tcagcaggcg catcaccgaa gcgaaaccga caatccagcc
    22321 gataaagggc agcagcagcc atgagtcgag cgaggcgaga atcacgccgg aggtaatgaa
    22381 atacaccgac acataaacga ccatatcggc aaccgtcatc accgcgtcgc gcaacgccag
    22441 cgcggtctgc atgactttgg cggacacgcg tccggcaaat tcgtcctgat aaaaaccgag
    22501 gctttggttc agcatcaggc ggtggaagtt ccagcgcagg cgcataggga acacgccctg
    22561 aagggtttgc aggcgcacgt tggacgcggc atacgcccac acgaccgaaa acaccatcat
    22621 cgccgccatt gccgccagtt cccaaccttt ttcggcaaac agttcggcgg gcgcgtattt
    22681 gccgagccac tccacgattt tgcccataaa ttgaaaaacc agggcttcca taatgccgat
    22741 gccggcggtc agcgcagcca gggcggctat ccatttccgc acgccggcca tgctgctcca
    22801 gacaaaccgc cacaagcctt tttctggcgt tttcggggcg gcttcgggat aagggtcgat
    22861 tcgggactcg aaccaggaaa atattttgtt caacattgtt ttcgatttcg gtaaaacagt
    22921 ttcagacggc atcaaacaca atgccgtctg aaaggaagga caataacgcc attttacggg
    22981 aaaagccgtc gggaagacag cgcgaggcgg aaacgcaggg tttcgtcagg gcaaacgccg
    23041 cgccgccttc
//
LOCUS       DAWHXS010000020        22943 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWHXS010000020 DAWHXS010000000
VERSION     DAWHXS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.020"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(52..999)
                     /locus_tag="WIE22_000602"
     CDS             complement(52..999)
                     /locus_tag="WIE22_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4126739.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            1328..2716
                     /locus_tag="WIE22_000603"
     CDS             1328..2716
                     /locus_tag="WIE22_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ4126740.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYIKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
     gene            complement(2991..3191)
                     /locus_tag="WIE22_000604"
     CDS             complement(2991..3191)
                     /locus_tag="WIE22_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126741.1"
                     /translation="MPSEGLSDGIFAFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(3205..3759)
                     /locus_tag="WIE22_000605"
     CDS             complement(3205..3759)
                     /locus_tag="WIE22_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126742.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(3844..4344)
                     /locus_tag="WIE22_000606"
     CDS             complement(3844..4344)
                     /locus_tag="WIE22_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126743.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDAQDFSPMFLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            4748..5941
                     /locus_tag="WIE22_000607"
     CDS             4748..5941
                     /locus_tag="WIE22_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ4126744.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNTQMHKLWHISNIYTTRPAQELAQKLVKNSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHALTEFA
                     AANRP"
     gene            complement(6006..7250)
                     /gene="clpX"
                     /locus_tag="WIE22_000608"
     CDS             complement(6006..7250)
                     /gene="clpX"
                     /locus_tag="WIE22_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ4126745.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            7441..7812
                     /gene="rbfA"
                     /locus_tag="WIE22_000609"
     CDS             7441..7812
                     /gene="rbfA"
                     /locus_tag="WIE22_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ4126746.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPIED"
     gene            7823..8337
                     /locus_tag="WIE22_000610"
                     /pseudo
     CDS             7823..8337
                     /locus_tag="WIE22_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8395..9315
                     /gene="truB"
                     /locus_tag="WIE22_000611"
     CDS             8395..9315
                     /gene="truB"
                     /locus_tag="WIE22_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ4126747.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDIA
                     EFDSPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            9532..10032
                     /locus_tag="WIE22_000612"
     CDS             9532..10032
                     /locus_tag="WIE22_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126748.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPAI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRSNSQPASQPASQPASQPASQPASQP
                     ANYTLN"
     gene            10266..11738
                     /locus_tag="WIE22_000613"
     CDS             10266..11738
                     /locus_tag="WIE22_000613"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ4126749.1"
                     /translation="MIYIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFMKNRLSIAKEL
                     LTDDGIIVISIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFAGTHEYT
                     LVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDRPEMFYP
                     FLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIADKQWGRW
                     RWGYSIKNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEYSSGNGT
                     EQMKNLFGEKAFKNPKPEELIQDFITITTNESDIVLDYHLGSGTTAAVAHKMNRQYIG
                     IEQMDYIETLAVERLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACE
                     DSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQEPEFQSLALDEQKQMVLEMLDLNQMY
                     VSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQAEKKDGE"
     gene            11728..14508
                     /locus_tag="WIE22_000614"
     CDS             11728..14508
                     /locus_tag="WIE22_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ4126750.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHSKFLFTEKILQGDQVIPIHKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATPPEKADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     VDESKADYLAFLNWVENVQADDFSFLKTFSMSLNDSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWIKQNYQKHNIIITNSETNKSKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     GRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFAFED
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFAANKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLD
                     IQNRNELQNNLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDKPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDIKTAIAMQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFANGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(14652..15824)
                     /locus_tag="WIE22_000615"
     CDS             complement(14652..15824)
                     /locus_tag="WIE22_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ4126751.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            16115..16561
                     /gene="iscR"
                     /locus_tag="WIE22_000616"
     CDS             16115..16561
                     /gene="iscR"
                     /locus_tag="WIE22_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ4126752.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            16590..17804
                     /locus_tag="WIE22_000617"
     CDS             16590..17804
                     /locus_tag="WIE22_000617"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ4126753.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPAHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            18065..18451
                     /gene="iscU"
                     /locus_tag="WIE22_000618"
     CDS             18065..18451
                     /gene="iscU"
                     /locus_tag="WIE22_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ4126754.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            18538..18858
                     /gene="iscA"
                     /locus_tag="WIE22_000619"
     CDS             18538..18858
                     /gene="iscA"
                     /locus_tag="WIE22_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ4126755.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYINPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(18818..19039)
                     /locus_tag="WIE22_000620"
     CDS             complement(18818..19039)
                     /locus_tag="WIE22_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ4126756.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            19121..19621
                     /gene="hscB"
                     /locus_tag="WIE22_000621"
     CDS             19121..19621
                     /gene="hscB"
                     /locus_tag="WIE22_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ4126757.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            19719..22469
                     /gene="gyrA"
                     /locus_tag="WIE22_000622"
     CDS             19719..22469
                     /gene="gyrA"
                     /locus_tag="WIE22_000622"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ4126758.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            22626..22943
                     /locus_tag="WIE22_000623"
                     /pseudo
     CDS             22626..22943
                     /locus_tag="WIE22_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6369 a         5821 c         5420 g         5333 t
ORIGIN      
        1 aaagcgcaat cgaaaaagcc gccggaagat tccggcggct ttttcgcacc cttaagattt
       61 gtggcggatt tcccagcacc tatggatttt tttgttgcgg aaatcttcgg gaacggattg
      121 tttggaaatg tctttgacgg cgtattgttc cgataccaag tcgtctaaga caaagctgcg
      181 caggttgttg gaaaagtaca aaatgccgtc tgaagcgagc agcttcaccg cgccgtcaat
      241 cagctttttg tggtcgcgct ggatgtcgag gatgtcggac attttcttgc tgttggaaaa
      301 actgggcggg tccatcacaa tgaggtcgaa ccgcctgcct tccccatatg ccgtctgaag
      361 atattggaac acgtcggcgc ggacgatttt atgccgttcc gtatcgatgc cgttcaattc
      421 aaaattgcgt ctcgcccaat cgagataagt gttagacaaa tcgacagttt cgctggatgc
      481 cgcgccgccg gtggcggcat agacagtgaa gctgccggtg taggaaaaca ggtttaaaaa
      541 acgtttgccc gccgccgttt cgccgacttt tttgcgcgtg tttcgatgat ccaaaaaaag
      601 ccccgtatcc aaatacttat caaggttgac ccaaaacttg cggccgtttt cggtgatgac
      661 gaaatcgtcg cccgccttgc cggttttttc gtattgctgc aaaccttttt ggcgttcgcg
      721 gcgtttgagg cggatttgtt cgggcgcaaa accggtaacg aaggcgacgg cttccaagac
      781 ttcggcaagc cacgcttcgt attcttcggg ccgcatcagc cagccggtat cgtattcctg
      841 aaggtggatt cgatcgccgt aaacatcggc ggcaaagggg aattggggga tgtcgcggtc
      901 gtaaatgcgc caggcttcga tgccgttgcg tttcgcccat ttcataaggt gtttgatgtt
      961 tttgcccaag cggttggcaa acggtgtgat gtcggtcatt ggtttcaggc ggaataaagt
     1021 ggaaaacggc aattttactg taattaacgc ccgattgctt gaccgtttcg ggcaaaccct
     1081 ataccatccg ccgcttatct tgtcatacga agccatcgcc ttccaaccta aaccgccctt
     1141 acgggcgcgt ttcttttgtt gcttgcatat ttgccaagcc tttctgtgca ggttgtcgtc
     1201 gatgtaaacc acaagcaagc cgcttgcgac aaccctgtaa cttcacattc cccgtatcgt
     1261 tacccttccc tgcttcaggc cgtctgaacc tttcggacgc gggcgttgtt gtcttccaag
     1321 gatagccatg tctattaaat ttgccgattt gaacctcgat aaaaacatct tgtccgccgt
     1381 cagcagcgag ggttacgaaa gcccgacgcc gattcaggcg caggcgattc cgtttgcttt
     1441 ggaaggccgc gacatcatgg cttcggcgca aaccggctcc ggcaaaaccg ccgcctttct
     1501 gttaccgact ttgcaaaaac tgaccaaacg cagcgaaaaa ccgggcaaag gcccgcgtgc
     1561 tttggtgttg accccgaccc gcgaattggc ggctcaagtc gagaaaaacg cgctggcgta
     1621 tgccaaaaat atgcgttggt tccgtaccgt cagcatcgtc ggcggcgcat cgttcggcta
     1681 ccaaacccgc gccctgagca aaccggtcga tctgattgtc gccacgccgg gccgtctgat
     1741 ggacctgatg caaagcggca aagttgattt tgaacgtttg gaagtgctga ttttggacga
     1801 agccgaccgc atgttagaca tgggctttat cgacgacatc gaaaccatcg tggaagcaac
     1861 gccgagcgac cgtcagactt tgttgttctc cgccacttgg gacggcgcgg tcggcaaact
     1921 ggcgcgcaaa ctgaccaaag accctgaaat catcgaagtc gaacgcgtgg acgaccaagg
     1981 caaaatcgaa gaacaactgc tgtactgcga cgatatgcgt cacaaaaacc gcctgctcga
     2041 ccacatcctg cgtgatgcca acatcgatca atgcgtgatt ttcacgtcca ccaaagcgat
     2101 gaccgaagtc atcgcggatg aattgtacga aaaaggtttc gccgccaact gcctgcacgg
     2161 cgacatgccg cagggctggc gcaaccgcac gctgatggat ttacgcaaag gccgctgcaa
     2221 aattttggtt gccaccgacg ttgccgcacg cggtatcgac gtaccgacca ttacccacgt
     2281 catcaactac gacctgccga aacaggcgga agactacgtc caccgcatcg ggcgcaccgg
     2341 ccgcgcaggc cgcacgggta ttgcgattac gtttgccgaa gtgaacgaat acatcaaagt
     2401 ccacaaaatc gaaaaataca ttaaccgcaa actgcccgaa ctgaccatcg aaggcatgga
     2461 accgacccgc aaacgcaaat ccgcaggcgg taagccaaaa ggcaaaggcg gctggggcga
     2521 taggaaatcc ggcggccggc gcggcgatca taaaccgggc aaagaaggct tcggcggcaa
     2581 aacgcgcggc gaaggtttca agaaagaagg ctttaagaaa gacggtttca aaaaaaccgg
     2641 cgaaggcttc aaaggcaaac gtaaagccgg cgattctttt gcaggcagag gcgaacgccg
     2701 ttacaaagac cgctaagccc caacctgccg cataaaccaa tgccgtctga aaccgatttc
     2761 gggtttcaga cggcattttt gcaatgtttc aacaccgccc ggctttgata cccaaaggat
     2821 taggctgtaa taaaaaccct tttccgcttt ggcaacgatt gaaaatttcc gtaaattcaa
     2881 atatctagat tccttcctgc acgggaatga cacggaaggg tttcagatgc agggtgggca
     2941 ttcctgccca cccaatcccg cccttgcaac ggtgggcaag aatgctcgcc ctacggcttg
     3001 actgttcgat atgatgccgt ctgaaaaccc aacggcggca tgacaatgcc accctgccaa
     3061 cgcacgtaaa tcagaattgc catcccgaca tcaaacgctt ggaaacaaaa tgccgtctga
     3121 aaatcaaacg gcaacataac aatgccccta acaaatgcaa aaatgccgtc tgaaagccct
     3181 tcagacggca ttggcacgcc gggtttaccg cctcctgccg aaaccgcgca tagcggggcg
     3241 gcggtaattg gcgggcgggc gcgttgtcgg gcggtaacgc tgcgcctgcg ccgcctgttg
     3301 ttttgcacgg aggctgcgcg tgttcaaatc cctgctggtg cgcgcattgg gatgtgcgga
     3361 ctgatggtag gcggcacgcg cgcgtcgggc gacgggactg tcttggttgc ccgcccgtgt
     3421 gaatttgttt gccagcgcgt tgccgataaa cgcgcctgcc gccgcgccga ccaggctttg
     3481 cagcagccag cttcctgtcg attggtcgta aatatactgc tgcccgtctt taccggtaac
     3541 gggttgcccg ttgttgccgt ttgcctgtgc ttcggcagga atggtgtctt tgactgcttc
     3601 gggagtcagt tggtaaaccg tatcgtctgc ctgctgtgcg agctgctgtt gcagggcttc
     3661 aatctgtttc tgctgctgtt cgagccgcgc ctgcgtgtcg tcttggcagg cggcgagtgc
     3721 gaatgttgcg ataagcgcgg aggcgatgat ttttttcatg tgtgtcctgt ttgggtggaa
     3781 aatcggtttt attgtatcgc cgtcgggaat tttggcaagc attctgccga caaatcgtga
     3841 tgtttacagg ggcagggtgt gcagtttgcg gacaaatgcg aggctgttgg cgactgggtt
     3901 gcctttgttt tcgacttcgt gttcggtttc tacggtcagc aggcggcggt ttttgtgttt
     3961 gttcaggctc aattcggctt gtgcgggtgt gaaaaacagg cggtgttttt cggcaaagcg
     4021 gcgggcgcgg ctgttggcgg ttttcaaaat ctgaagcagc gatacgtcca gatggaagcg
     4081 tcgcacgccc aatacgagaa tccgtgcggc aaaaaaatcg gcggcatcgg taaactcggc
     4141 ggggctgctg cggctgaagt cgtgccgttc ggcggctatc agggcggcaa cggtgcggat
     4201 ttgcggaatc atcgattcgc tgatgagtgt gtcgtcccgc cctgcatcga gaaacatggg
     4261 ggaaaaatcc tgcgcatcat cgacaataat gctgcaaatg tgcagggttt cgttttgtgc
     4321 ggcggtttgg ggcattgcat tcatggtcat tttcctgatt ctgtcgtgtg ttgccgaatc
     4381 gggcgacctg tgtgaaggta acaaaaaagc cgccccattt tcgagcggcc tgttttgcgt
     4441 atgggatgga tttcaagcaa gcgcaaaaaa gtaccgcacg tctgtgtggt accaatagca
     4501 ataagcggtt gtaaattttt tgccttgcat gatgaaatgc cgtctgaaga taaaaatatt
     4561 ggggagattc taaatcaaaa cgctgccgcg cctcaagcat tttatcgaaa tttttttgat
     4621 ttttcatcta tccgattgaa aatatttcgg tttattttta ccgctgcccg atattgtcgg
     4681 caatttccct ttatctgctt tgaaaaacgg tgcataatcc cgagcaaaac cgcaatcagg
     4741 agcaattatg caaaactatc tgacccccaa tttcgccttt gcaccgatga ttcccgaacg
     4801 cgcttcaggc agccgcgttt gggatacgga agggcgtgaa tatattgact tttcaggcgg
     4861 catcgccgtc aatgcgctgg gacactgcca ccctgccctt gccgatgctt taaacacgca
     4921 gatgcacaag ctgtggcaca tttccaatat ctatacgacg cggccggcgc aggagttggc
     4981 gcaaaaattg gtgaagaaca gctttgccga caaggttttt ttctgcaact cgggcgcgga
     5041 agcgaatgag gcggcgttga agctggcgag gaaatacgcg cgcgaccgtt tcggcggagg
     5101 aaaaagcgaa atcgtcgcct gtatcaacag tttccacgga cgcacgctgt ttaccgtgtc
     5161 cgtcggcggc cagccgaaat acagcaagga ttatgcaccc ctgccgcaag gcattacgca
     5221 cgttccgttc aacgatattg ccgcgctgga agctgccgtc ggcgaacaga cctgcgcggt
     5281 catcatcgag ccgatacagg gcgaaagcgg catcctgccc gccactgcgg aatatttgca
     5341 aaccgcgcgc cgtctgtgcg accggcacaa tgcgttgttg attttggacg aagttcaaac
     5401 cgggatgggg catacgggca ggctgtttgc ctatgaacat tacggcgttg ttcccgatat
     5461 tttgagttcg gcaaaagcct tgggctgcgg ctttccgatc ggcgcgatgc tggcgacaga
     5521 aacgattgcc gccgccttcc aaccgggcac gcatggctcg actttcggcg gcaacccgat
     5581 ggcgtgtgcg gtcggcagcc gcgccttcga catcatcaat gcgccggaaa ccttgcacaa
     5641 cgtccgcagt caggggcaga aacttcagac ggcattgctg gatttgggca ggaaaacagg
     5701 cttgttctca caggttcgcg gaatgggact gctactcggc tgcgtgttgg acgaagccta
     5761 tcgcggacgc gcatccgaaa tcaccgccgc cgccttgaaa cacggcgtga tgattttggt
     5821 tgcaggtgcg gacgtattgc gtttcgcgcc ttcgctgctg ttggacgatg aagatacggc
     5881 ggaaggtttg cggcgtctgg aacacgcgct gacggaattt gccgccgcaa accgccccta
     5941 ggcatcagaa acaaaaaacc gcttcggaaa cgtggtttaa cgttccgaag cggttttgtt
     6001 tgccatcagg actcgaacac caattccggt tccctgccct cttcaatgac tgccttaccg
     6061 accaccactt ttttcaagcc tttcaaatca ggcaggcggt acatggtatc gagcaggcag
     6121 cgttcgacga tggaacgcag gccgcgcgcg ccggttttgc gctccattgc ctgacgcgcg
     6181 atggaacgca atgcgccttc ttcaaattcc aactcgacat tttccatacc gaacaaggct
     6241 tgatactgct tgaccaaagc atttttcggc tcggtcaaaa tattaatcag cgcgtcctca
     6301 tccagttctt ctaacgttgc aatcacaggc aaacgtccga ttaattccgg aatcagaccg
     6361 aatttgatta aatcttccgg ttcgacgatg ccgaacagct tggtaatgtc ggcattttcg
     6421 tccttgctgt gaacggacgc accgaaaccg ataccgcctt tctcggtgcg ctggcggatg
     6481 actttttcca agcctgcaaa cgcaccgccg cagataaaca ggatgttggt ggtatcgacg
     6541 ttgataaatt cctgattcgg atgcttgcgg ccgccttggg gcggaacact tgccaccgta
     6601 ccttcaatca gtttcagcaa ggcttgttgc acaccttcgc cggacacgtc gcgcgtgatg
     6661 gacggattgt cgcctttgcg tgaaatttta tcgatttcgt cgatatagac gatgccgcgc
     6721 tgggcttttt caacatcgaa atcacatttg cccaacagtt tggtgatgat ttgctcgaca
     6781 tcttcgccga cataacctgc ttcagtcagc gtggttgcat ccgccatcac gaacggtacg
     6841 tccagtttac gcgccaaaga ttgcgccagc agggttttac ccgatccggt cgggccgata
     6901 agcaggatgt tggatttcga caattcgaca ttagctccgg ctttcgggtg gcgcaggcgt
     6961 ttgtaatggt tgtacaccga caccgccaag gctttcttgg cttgttcctg accgataacg
     7021 tggtcgttga ggttggcgac gatttcggcg ggcgtgggca gcttgccgga ttcttccggc
     7081 tcccctccgg cactttccga aggtgtgccg tcatttccgt cttcatgcaa tatttcaata
     7141 cagtttgaga cgcattcgtc acagataaag gcgttttcgc cctcaattaa atgtttgacg
     7201 tgtgatttgg attttccgca aaaggaacag gtacggtttt cgttggacat ggctttcttt
     7261 acaatgtatg cgttacagaa aacggcacgt gccgttcggg ttgccaagta taataactat
     7321 atccgttctt atcaatgtat taccttaaaa tcccgccgat taggctataa tacgcccttt
     7381 cgcaaccgcc ccggcggcaa aaatgccgtc tgaaaccaaa tctgaaatct gaggatattc
     7441 atgagaaaac cccaacgcgg ctatgcccgc caagaccgtg tcaaagaaca aattatgcgc
     7501 gagcttgccg aactcgtccg taccggactg aaagacccgc gcgccggctt cattaccgtc
     7561 aacgaagtcg aagttacccg cgattacagc cacgccaccg tgttctacac cattttaaac
     7621 caagacgcgc gcgaaattac ggaagaagtg ctggaacacg cgcgcggaca cctccgcagc
     7681 gaattggcca aacgcatcaa gctgttcaaa atccccgagc tgcatttcaa atacgacgaa
     7741 tcgttggaac gcggtttgaa cctgtccgcc cttatcgatc aagtggcggc ggaaaaaccc
     7801 atcgaagact gatactgcta aaatgaacaa tctgattccc gaacatttag ccgcctatgc
     7861 acatagtgac agcctgcaaa ttgaaggcgt gcatcgttgc ttttcattat cctgccaagg
     7921 tagagatact ttccacatcc gttactatgg agagcctttt gatggattga tgaccgatac
     7981 tgataaggct ccggtaaaaa ttgtggcagt agaagctgta agcggcaatg aaatcgtatt
     8041 gtttgatggc gcggaacatg gctataacgc tatgttttgc gacaaatata gtccaaatca
     8101 aaagcaaaac agaacgttaa ctgatttgga tgagtatact taccgggttc tgatccatct
     8161 ttattacaat ataaactacg aagatgaata tgaagatttc gtcaattctg aaggacaagt
     8221 ccccttaatt gatggccgaa tcattagttt tgactcatta aaacgaaatc aatcagcgtt
     8281 gatctaattg atgaaaaaca atctgttcgt gaattattga atgaagaatt aagctgacac
     8341 gcaggccgtc cgaacccttt cagacggtct gaacattaat attccctaat taatatgacc
     8401 aataaacccg ccaaacgacc ggtcaacggc gttcttcttc tcgacaaacc cgaaggcctt
     8461 tccagcaaca ccgcgctgca aaaagcgcgg cgtttgtttc atgccgaaaa agccggacat
     8521 accggcgtgc tcgacccttt ggcaaccgga cttttgcccg tctgcttcgg tgaagcgacc
     8581 aagttcgccc aatacctgct ggatgccgac aaagcctaca ccgccacgct gaaactcggc
     8641 gaagccagca gcacgggcga tgccgaaggc gaaatcgttg ccgccgcccg cgccgatatt
     8701 tccttagccg aatttcagac ggcctgccaa gcactgacag gcaacatccg ccaagtgccg
     8761 ccgatgtttt ccgccctcaa gcacgaaggc aaaccgctgt acgaatacgc ccgcaaaggc
     8821 atcgtcatcg aacgcaagcc gcgcgatatt accgtttacg ccatcgatat tgccgagttt
     8881 gactcaccca aagccgtcat cgacgtgcgt tgcagcaaag gcacctatat ccgcaccctc
     8941 agcgaagaca tcgccaaaca catcggcacg ttcgcccacc tgaccgccct gcgccgcacc
     9001 gaaaccgccg gctttaccat cgcccaaagc cacacgcttg aggccttggc aaatttgaac
     9061 gaaacagaac gcgacagctt gctgctaccc tgcgacgtat tggtttcaca ctttccccaa
     9121 accgttttaa acgattatgc cgtccatatg ctccactgcg gacaacgtcc gcgtttcgaa
     9181 gaagacctgc cttccgacac gccggtacgc gtttacacgg aaaacggccg ctttgtcggt
     9241 ctggcggaat atcaaaaaga aatatgccgt ctgaaagcct tgcgcctgat gaacacggcg
     9301 gcatccgccg cctgaacggc ggttaaaaat acaggctgtg cttgaataat gtgttgatat
     9361 ttccgcaaaa tcccgacaca ctcggacacc cgccccgctt atcgcaactt tgcgaacgcc
     9421 cccagaaaca gcaaagacat caaaggattg attttattag aatttatttg caaagccatt
     9481 tgccgttaca caagaatggc acattaaaat gactgatgag gatttataac gatgaagaca
     9541 gacattcaaa ccgaattaac ccaagccctg ctaccacacg aaaaagtatg ggcgaacgaa
     9601 gaaaaaacca ttttagccaa aaacattctg ttggatttgg tggaaaaaac cgacccggcc
     9661 attatcggtt tgttattgaa taatgatgag ttaaaacgcc atttctttgt ggaagtgaat
     9721 ggtgttttgg tgtttaaatt gcaggatttt cgttttttct tggacaaaca cagcgtcaat
     9781 aattcctaca caaaatacgc caaccgcatt ggtttgacgg acggcaaccg ctttttgaaa
     9841 gacaattcgg atattgtgtt ggattttccg tttaaagatt gtgtattgaa tggcggacaa
     9901 agcaccgagg aaggcgaaga gatttatttt aaacgcagta atagccagcc agccagccag
     9961 ccagccagcc agccagccag ccagccagcc agccagccag ccagccagcc agccaattat
    10021 acactaaatt aacccgaaaa agacaagaaa tcttttttaa tcaaacgctt gcttttgatg
    10081 aaatcgatcg gctttttgac gcaaaagcat tctcaaaatt ctctcgctat accgcagacg
    10141 gcaaacaagc ggttggcgaa atcaaacgtc attcagacgg cacacccgcc gaaaatctca
    10201 ttatcaaagg caataatctg atagccctac attcgcttgc caaacagttt aaaggcaaag
    10261 tgaagttgat ttatattgat ccgccttatt attttgttaa aaagaaacaa caagatagtt
    10321 ttggttataa cactaacttt aaattatcta cttggcttac atttatgaaa aatcgtttat
    10381 caattgccaa agaattatta actgatgatg ggattattgt tatttccatt gatgatgacg
    10441 gaaatgctta tttaaagatt ttgttagatg aaatttttgg atttgaaaat tttatcggaa
    10501 atttacccac tattatgaat ttaaaaggta ataatgatga atatgctttt gcaggcactc
    10561 acgaatacac actagtcttt gctaaaaata aagataaatc tactttttat gaatttccaa
    10621 tagatgaaga taatttttta gagaaatggg aagaagatga aattgggttt tataaaaaag
    10681 gagctccaat gcgagcaaca ggaaccgaag ataaaagaga agatagacct gaaatgttct
    10741 atcctttttt ggtaaaaaat aacacagtta gtacaattac agatgaagaa ttttctcaaa
    10801 tctataataa agatttagaa gtattcaatg atgattttat tcaaaaacta aaagagaaat
    10861 acgaaaatct tggatacaat tttattttac ctattgctga taaacaatgg gggcgttggc
    10921 gttgggggta ttcaataaag aataaggcaa ggctgcagac ggatattatt gttagccaat
    10981 ctaaaaatgg aatttccttg tataaaaaac aaagacctga attagatgat ttacctacaa
    11041 aaaaacctaa aactattttt tataaacctg aatacagtag tggtaatgga actgaacaga
    11101 tgaaaaatct ttttggtgaa aaggcattta aaaatcctaa accagaagaa cttatccaag
    11161 attttattac catcacaacc aacgaatccg acatcgtctt agactaccat cttggtagtg
    11221 gcacaaccgc cgccgttgcc cacaaaatga accgacaata tatcggtatt gaacaaatgg
    11281 attatattga aacgcttgcc gttgaacgct tgaaaaaagt gattgatggc gagcaaggcg
    11341 gtatttccaa agccgtgaat tggcaaggtg gcggcgagtt tgtctatgcc gaacttgctc
    11401 catttaacga aaccgcaaaa caacaaattt tggcttgcga agattcagac ggcatcaaaa
    11461 cgctgtttga aggtttatgc gaacgctatt tcttgaaata caacgtcagc gtaaatgaat
    11521 ttagtcaaat cattcaagag cctgaatttc aatccttggc attagacgaa caaaaacaaa
    11581 tggtgcttga aatgttggat ttaaatcaaa tgtatgtttc attatccgaa atggatgatg
    11641 agcaatttgc aggttgctta aatgatgacg ataaagcgtt aagccgtgaa ttctatcaat
    11701 cagcaaaaaa tcaagcggag aaaaaagatg gcgaataata aaacgttatt tgattgggtg
    11761 gaagaccgca aatcaacgct ggaagaaata gaacagacag attttttcgc gctgcctgaa
    11821 tttgtagaac gcaatttaaa atatccgttt tttgaatggc aaaaatcggc tttagaaaac
    11881 tttgtgattt ttgaccgcgc ttcaaaatta aaggatttcc ctgacatcaa aaaccgccct
    11941 acccatttgc tgttcaatat ggcaacaggt gctggcaaaa cgatgatgat ggcggcgttg
    12001 attttgtatt attttgaaaa aggttatcgg cattttttgt ttttcgtcaa tcaaaataat
    12061 atcgtggata aaacggaaaa taattttatt gacccggcgc actcaaaatt tttatttacc
    12121 gagaagattt tgcaaggcga ccaagttatt cccattcaca aagtagaaac gtttagccca
    12181 cattcagacg gcattgaaat taaatttacc agtattcaaa aactgtataa cgatatccac
    12241 accgaacggg aaaaccaaac cacattggca gatttgcacg aattgaacct tgtgatgctg
    12301 ggcgatgaag cgcaccattt aaacgcacaa accaaaggca aaaaacaagg cgagttggat
    12361 ttggaagtgg aattaaaagc caatgcaggc gatgccgaat ttgaacgcaa aggctgggag
    12421 catatggttt tggaattgtt gctcaataaa aatggcaatc ccggccaaaa tgtgctgttg
    12481 gaatttaccg ccacgccacc tgaaaaagct gacgtgcaac aaaaatacgc tgataaaatc
    12541 atcacaaaat ttggtttaaa agaatttttg caaaaaggtt ataccaaaga aatcaatttg
    12601 gtatccagta cactgagcaa gaaagagcgg gtgttgcacg ctttattgtt tgcttggtat
    12661 cgccatcaaa tcgctttgaa atatggcatt gccaatttca agcctgtgat gttgtttaga
    12721 agtaagacgg ttgatgaatc aaaagcggat tatttggcat ttttaaattg ggtagaaaat
    12781 gtgcaagcag atgatttttc gtttttaaag acattttcaa tgagcttaaa cgatagcgat
    12841 aacgccaacg aacaaggcaa aacccgcacc gaacaagccc taaaatttat gcaggaaaat
    12901 ggctttgagt ttgcacattt agcaaattgg ataaaacaaa attatcaaaa acacaatatc
    12961 attattacca actccgaaac caacaaaagc aaaaccgaaa aaaccgacag cgaaacggaa
    13021 aaattgctaa acaatctgga agcggctgat aatccaatcc gtgccatttt tacggtgggt
    13081 agattaaccg aaggttggga cgttttaaat ttgtttgata ttgtgcgttt gtatgaaggg
    13141 caaaacggcg gcggttcaaa taaaaaatca tgcaaaacgg caaatacaac ggtatcagaa
    13201 aaacagttga ttggtcgagg cgtgcgttat tttccatttg cgtttgaaga taaacagccg
    13261 aataagcgta aatttgacaa cgacatgcaa cacgaattgc gtattttgga agaattgttt
    13321 tattacacgc atgatgagca atcccgctat atttcagaac tgaaaaacga gttgcgaaaa
    13381 gacggttatt tgcctgaaaa agacgatgat aaggtattgg caacatttaa actcaaatct
    13441 gaatttgccg ctaataagga ttttagagag ttgttaattt gggcgaataa aaaaatcccc
    13501 aacccaaacg ccaaaagcaa taatgcagac agcctgcaag ccaatccgca aacgctttca
    13561 ttccaagttc acggtaatca actgttacag gaaacgcaat ttacagccga tgaaaatgat
    13621 gaaaccgccc gacaaatcgg cacacaaaat aattttactc aaaccataaa aatgagtgaa
    13681 atggagcggc acattttcaa taaagcatta catatcaaag ggaaaaatag tcaatcttta
    13741 ttccatttca accgtttgca aagcaagctc gacattcaaa atcgcaatga attgcaaaat
    13801 aacttgttga aagactggca aattgaattt ttaggattag agcaagataa acaggttcgc
    13861 ccggatgata aacttgcagg ctgcctgaaa atcttggaaa tggttgaaaa gcatttgaat
    13921 gaaagtgata agccatttat cggtacaaaa gaatttacgc ctaaaacatt atgggaaatt
    13981 ttcggcacgc caaaacaaaa atgggtcaaa aaagatgata taaaaactgc tattgccatg
    14041 caaaatgatt ggtatgtgat ggataatttt gccggaacgg gtttggaaga agcgttaatt
    14101 caatttattt cagcgcgttt gggcgatttg aaatctcaat atgacattca tttaatccgt
    14161 aacgaagaag tgtttaaatt gaataacttt gccaatggcg aaggatttat gcccgacttt
    14221 attttattgc tgaaagataa acaaaaatct tcttccaatg gcgtgaatga ctttttgcat
    14281 taccaaattt tcattgagcc caaaggtgag catttagtgg aaactgatgg atggaaagaa
    14341 gaatttttag aagccatcac agtggaatat gggatggata agatcctgca aaaagataca
    14401 ccgcattatc gtttgatcgg tttaccgttt tttactgatc atcagaaaaa tggacaattt
    14461 acggagtcat tccctttagg tgcggcatcg cttgaaaaaa tgaagtagtg cattgcaggc
    14521 agccccgttt gacaaaactt cctttacaaa agggcgtttt gtcagatatt taatcaacac
    14581 attattaaaa tacagccaaa ttttaatgcc gtctgaaccc tgtgttcaga cggcatcgta
    14641 tttttcagta tctaaaccgt ttccctaccc caatccttac ccctcaaaat cgaggcatcg
    14701 acatcttgaa tatcgcggtg tcccgtaaac gccatagata tatccatttc tttatacagg
    14761 atttccagcg cacgggttac gccttcttcg ccatacgcac ccaagccata cagaaacgcc
    14821 cgacctatca ttgtaccttt cgcacccaaa gcccacgcct tcaaaatatc ctgaccgctg
    14881 cggatgccgc tgtccatcca aacttcgatg tcgctgccga ctgcactaac gatgtcgggc
    14941 aaggctttga tggcagacac ggtatcgtcg agctgtcggc cgccgtggtt ggaaacaatc
    15001 aatgcgtccg cgccgctttt cgctgctttt tccgcgtctt cgggttccat aatgcctttg
    15061 ataatcagct tgccgcccca caaatcttta atgcgcgcca catcgtccca gctcaggcgc
    15121 gggtcgaatt gttcggaagt ccatgaagac agcgaagaca aatcgccgac gttcttcgcg
    15181 tgtccgacga tattgcggaa cgtgcggcgt tccgtgttca gcattttcat gcaccattcc
    15241 ggtttggtcg ccagattgat taaattggcg atggtcggtt tcggcggcgc ggacaggccg
    15301 tttttgatgt ctttgtggcg ttgacccaaa acctgcaaat cggcggtcaa taccaatgcc
    15361 gaacatttgg catccttcgc gcgcttaatc aggttttcca taaactcgcg gtcgcgcatc
    15421 acataaagct gaaaccaaaa tggtgcggaa gtgttctcgg caacgtcttc aatcgagcag
    15481 atagacatcg tggacagcgt aaacggaatg ccgaacttct ccgccgcccg cgccgccaaa
    15541 atttcaccgt cggcgtgtgc catacccgtg aaacccgtcg gcgcaatcgc caccggcatt
    15601 ttcacatcct gcccgatcat tttggtttcc aggcttcggc cttccatatt gaccaatacc
    15661 ttttgtcgga agcggatgtc tttgaaatcc gaagtgtttt cacggtaggt agtttctgtc
    15721 cacgaacccg aatcgatgta atcgtaaaac atacgcggca ttttgcgctt ggcaacgcgg
    15781 cgcaagtctt cgatgcaggt cattttgctc aaatcacgtt tcatttgtcg ccccctgaat
    15841 acctgaataa ctttatatga aatcgataat gtatatcaat attgattata aggcaaatca
    15901 tttcaacatt tgccgcatcc gccgcagctc cctactttaa gcgacataag gtttaaaatt
    15961 caaaaataac aaattaaaat caaaatatta aaaatcaatc aatctatcga tttaaacagc
    16021 caatcacaca atccgccccc atacttgact gaaacactca gatattggac aattccaccc
    16081 actaataaaa aaaccgacat gggcaaccac caccatgaga ctgaccacca aagggcgttt
    16141 cgccgttacc gctatgctgg atttggcgat gaacgcgcaa accggcgccg tcaaactcag
    16201 tgccatcagc gaacgccaaa acatatccct ctcctatctc gagcaattgt tcggcaaact
    16261 ccgccgcgcc ggacttgttg aaagcctgcg cgggcccggc ggcggctaca tcctcgccgc
    16321 accggcggca cgcatcaaca tcgcccaaat catcgccgcc gccgaagacc ggctggacgc
    16381 aacccaatgc ggcagcaaag ccaactgcca ccacggcgcg ccctgcctga cgcacgatct
    16441 ttgggagaat ttaaacaaaa ccatcaacga ctacctcggc agcgttaccc tgcaaagcat
    16501 catcgaacag aaaaacaacg gcgacggcag ccgcgtcgtc caatttacac acatccatta
    16561 aataacaccc gaaaaagaaa gagcaaacca tgaccgtcaa aacccccgtt tacctcgact
    16621 acgccgccac cacccccgtt gacaaacgcg ttgccgaaaa aatgattccc tatctgaccg
    16681 aaaccttcgg caacccagcc tccaacagcc acagcttcgg ctgggaagca gaagaagctg
    16741 tagaaaaagc acgtgcagac attgccgccc tgattaacgc cgactctaaa gaaatcgttt
    16801 tcaccagcgg cgcaaccgag tccaacaacc tcgctatcaa aggcgcggcg cacttctaca
    16861 aatctaaagg taatcacctc atcactgtaa aaaccgaaca caaagccgta ctcgacacca
    16921 tgcgcgaact cgaacgccaa ggttacgaag taacttatct ggacgtacaa gaaaacggtt
    16981 tggttgattt agacgtactg aaagccgcca tccgcgaaga caccatcctc gtttccgtaa
    17041 tgtgggtaaa caacgaaatc ggcgtggttc aagatattcc tgccatcggc gaaatctgcc
    17101 gcgaacgcaa aatcattttc cacgttgacg cagcacaagc atgcggcaaa gtgcctgttg
    17161 atgttgaagc cgcaaaagtt gatttgctgt ctatgtccgg ccacaaagta tacggcccta
    17221 aaggcatcgg cgcgctgtac gtccgccgca aaccccgcgt ccgcctcgaa gcccaaatgc
    17281 acggcggcgg ccacgaacgc ggcttccgca gcggcacatt gccggcccac caaatcgtcg
    17341 gtatgggcga agccttccgc atcgccaaag aagaactcga acaagatatg gcgcactacc
    17401 gcaaactgcg cgacatcttc ctcaaaggca tcgaaggcat cgaagaagtc tatgtcaacg
    17461 gcgacctcga acaccgcgcc ccgaacaacc tgaacgtcag cttcaacttc gtcgaaggcg
    17521 aaagcctgat tatggcggtg aaagaactcg ccgtatccag cggctccgcc tgtacttctg
    17581 ccagtctcga acccagctac gtcctgcgcg cgctcggccg caacgatgaa ctggcgcact
    17641 catccctgcg catcaccttc ggtcgcatga ccactgaaga agaagtgcaa tttgccgccg
    17701 aactgattaa atccaaaatc ggcaaactgc gcgaactgtc gccgttgtgg gaaatgttca
    17761 aagacgggat tgatttgaac tcgattgaat gggcggcgca ttaaagcgta ccaacatgcc
    17821 atccgaacct tcagacggca ttccaaaaac aaagcaatca agagaaaaat atgaacgaac
    17881 aagatttaga tttggacaac ctcgacaacc tgcttgacga tttcgacggc gttaccgtgg
    17941 aaggcggcgt cgattcggaa aacgacgacg gctgtgaagg cggagcgtgc aaaatctaac
    18001 tcaggccgtc tgaaaaccgg caaacaaacc acttaaatca tcaaaacatt aaggaaacca
    18061 catcatggca tacagcgata aagtaatcga ccactacgaa aatccccgca acgtcggtac
    18121 tttcgacaaa aacgacgaat ctgtcggcac cggcatggtc ggcgcaccgg cctgcggcga
    18181 cgtgatgcgc ctgcaaatca aagtgaacga tgaaggcatc atcgaagatg cgaaattcaa
    18241 aacttacggc tgcggttccg ccatcgcttc gtccagcctg attaccgagt gggttaaagg
    18301 caaaagcctg gatgacgcgc tggcaatcaa aaacagcgaa atcgccgagg agttggaatt
    18361 gccgccggta aaaatccact gctccatctt ggctgaagat gcggtaaaag cggccgttgc
    18421 cgactaccgc aaacgtcagg aaaacagata aagcccttca gacggcatcg tcccgcaatg
    18481 ccgtccgaac cacccgccgc ttcaggtccg tccggggcgg tacaacaagg aagaaatatg
    18541 attaccctta ccgagaatgc cgcaaaacac atcaatgact atctcgccaa acgcggcaaa
    18601 ggcttgggcg tacgcttggg tgtgaaaacc agcggctgct cggggatggc gtacaacctt
    18661 gaatttgtcg atgaagccga cggcgacgac ctgattttcg aaggacacgg cgcgcgcatt
    18721 tatatcaatc cgaaaagcct ggtttatctg gacggcacgc aagtcgatta caccaaagaa
    18781 ggtttgcagg aaggtttcaa atttgaaaac cccaatgtca aagactcctg cggctgcggc
    18841 gaaagcttcc acgtttaagg cataaaaacg gcgggacagt atcaaaaccg tcccgccatt
    18901 ttttcgcttc ctgcctgttg tagctgcctt tgcctttcct tttccgttcc accttgtgcc
    18961 ggaacaaatc ggatttcact aaggctttta aagcattgtc gcgtattttg cctttattgt
    19021 gctgcacttt gccgcccata ttcagtcctt tcgtttaaga agcggcagat tataaggcaa
    19081 aaacagtttt ctgccaaaat cttacattta tcattctact atgtcccaat atttcaccct
    19141 cttccggatt gaacccgctt tcgatatcga caccgaaaac ttggaacaaa cctaccgcgc
    19201 cttggccgcc cgtttccatc ccgataaatt cgcttcagct tccgcctttg agcaaaagca
    19261 ggcagtgatg atgtcttcca ccatcaacga tgcctaccgc accttgaaaa accccatcga
    19321 ccgcgccgcc tacctgctga aaacatcggg catcgatgcc gacgcgccgg agcataccgc
    19381 tttcgccccc gaattcctta tgcagcaaat ggaatggcgc gaaacgctga tggaggcacg
    19441 ggcaggcaac gaccttgaat ccttgaaaaa tctcgacaac gaaatccgcg acgaacaaga
    19501 aaaactgttc tgcggtctga aacagtcgtt tgcccgacaa gattacgaca cagccgcaca
    19561 acaagtccgc caaggcaggt ttctcgacaa actccgcaac gaaatttcct cggcattata
    19621 atccgcaccg tgtttcagac ggcgtaaccg ccgcaccgtt ccgcgtcaaa atatgctaaa
    19681 ataagcaaca attttttgcc atacgaaaca ttgaaaccat gaccgacgca accatccgcc
    19741 acgaccacaa attcgccctc gaaaccctgc cggtaagcct tgaagacgaa atgcgcaaaa
    19801 gctatctcga ctacgccatg agcgtcattg tcgggcgcgc gctgccggac gttcgcgacg
    19861 gtctcaagcc ggtacaccgc cgcgtactgt acgcgatgca cgaattgaaa aacaactgga
    19921 atgccgccta caaaaaatcg gcgcgcatcg tcggcgacgt catcggtaaa taccaccccc
    19981 acggcgatac cgccgtatac gacaccatcg tccgtatggc gcaaaatttc gctatgcgtt
    20041 atgtgctgat agacggacag ggcaacttcg gatcggtgga cgggcttgcc gccgcagcca
    20101 tgcgctacac cgaaatccgc atggcgaaaa tttcccacga aatgctggca gacattgagg
    20161 aagaaaccgt caatttcggc ccgaactacg acggtagcga acacgagccg cttgtactgc
    20221 cgacccgttt ccccacactg ctcgtcaacg gctcgtccgg catcgccgtc ggcatggcga
    20281 ccaatatccc gccgcacaac ctttccgata ccgtcaatgc ctgcctgcgc ctgctcgatg
    20341 cacccgacac cgaaatcgac gaactgatcg acattatcca agcccccgac ttcccgaccg
    20401 gggcaaccat ctacggcttg agcggcgtgc gcgaaggcta taaaacaggc cgcggccgcg
    20461 tcgttatgcg cggtaagacc catatcgaac ccataggcaa aaacggcgaa cgcgaagcca
    20521 tcgttatcga cgaaatcccc tatcaggtca acaaagccaa gctggtcgag aaaatcggcg
    20581 atttggttcg ggaaaaaaca ctggaaggca tttccgagct ccgcgacgaa tccgacaaat
    20641 ccggtatgcg cgtcgttatc gagctgaaac gcaacgaaaa tgccgaagtc gtcttaaacc
    20701 aactctacaa actgactccg ctgcaagaca gtttcggcat caatatggtg gttttggtcg
    20761 acggacaacc gcgcctgttg aacctgaaac agattctctc cgaattcctg cgccaccgcc
    20821 gcgaagtcgt tacccgacgt acgcttttcc ggctgaagaa ggcacgccat gaagggcata
    20881 tcgccgaagg caaagcggtc gcactgtcca atatcgatga aatcatcaag ctcatcaaag
    20941 aatcgcccaa cgcagccgag gccaaagaca aactgcttgc gcgtccttgg cgcagcagcc
    21001 tcgttgaaga aatgctgacg cgttccggtc tggatttgga aatgatgcgt ccggaaggat
    21061 tggctgcaaa catcggcttg aaagagcaag gttattacct gagcgagatt caggcagatg
    21121 ctattttacg catgagcctg cgaaacctga ccggcctcga tcaagaagaa attgtcgaaa
    21181 gctacaaaaa cctgatgggt aaaatcatcg actttgtgga tatcctctcc aaacccgaac
    21241 gcattaccca aatcatccgc gacgaactgg aagaaatcaa aaccaactat ggcgacgaac
    21301 gccgcagcga aatcaacccg ttcggtggcg acattgccga tgaagacctg attccgcaac
    21361 gcgaaatggt cgttaccctg acacatggcg gctatatcaa aacccagccg accaccgact
    21421 atcaggcgca gcgtcgcggc gggcgcggca aacaggcggc tgccaccaaa gacgaagact
    21481 ttatcgaaac cctgtttgtt gccaacacgc atgattattt gatgtgcttt accaatttgg
    21541 gcaagtgtca ttggattaag gtttacaaac tgcccgaagg cggacgcaac agccgcggcc
    21601 gtccgattaa caacgtcatc cagttggaag aaggcgaaaa agtcagcgcg attctggcag
    21661 tacgcgagtt ccccgaagac caatacgtct tcttcgccac cgcgcaggga atggtgaaaa
    21721 aagtccaact ttccgccttt aaaaacgtcc gcgcccaagg cattaaagcc atcgcgctca
    21781 aagaaggcga ctacctcgtc ggcgctgcgc aaacaggcgg tgcggacgac atcatgctgt
    21841 tctccaactt aggtaaagcc atccgcttca acgaatactg ggaaaaatcc ggcaacgacg
    21901 aagcggaaga tgccgacatc gaaaccgaaa tttcagacgg catcgaagat gaaaccgccg
    21961 acagcgaaaa cgcactgccg agcggcaaac acggtgttcg cccgtccggt cgcggcagcg
    22021 gcggtttgcg cggtatgcgc ctgcctgccg acggcaaaat cgtcagcctg attaccttcg
    22081 cccctgaaac cgaagaaagc ggtttgcaag ttttaaccgc caccgccaac ggatacggaa
    22141 aacgcacccc gattgccgat tacagccgca aaaacaaagg cgggcaaggc aatattgcca
    22201 ttaacaccgg cgagcgaaac ggcgatttgg tcgccgcaac cttggtcggc gaaaccgacg
    22261 atttgatgct gattaccagc ggcggcgtac ttatccgcac caaagtcgaa caaatccgcg
    22321 aaaccggccg cgccgcagca ggcgtgaaac tgattaactt ggacgaaggc gaaaccttgg
    22381 tatcgctgga acgtgttgcc gaagacgaat ccgaactctc cgacgcttct gtaatttcca
    22441 atgtaaccga accggaagtc gagaactgaa aatcatctcc cgatgccgtc tgaagattca
    22501 gacggcattt attttatccc tcatccgtca tccagcttct cacaatatag cggattatag
    22561 tcaattaaaa acaaggggct gtcctagata actagggaaa ttcaaattaa gttagagttg
    22621 cccctatgag aaaaagtcgt ctaagccggt ataaacaaat aaactcattg aactgtttgt
    22681 cgcaggtgta actgcaagaa cgacagcaga gttagtaggc gttaataaaa gtaccgcagc
    22741 ctattatttt catcgtttac gattacttat ttatcaaaac agtccgcatt tggaaatgtt
    22801 tgatggcgaa gtagaagcag atgaaagtta ttttgctgaa cgacaaaacc atatcaatgg
    22861 aattgagaac ttttggaacc gggcaaaacg tcatttacgc aagtttgacg gcattcccaa
    22921 agcgcatttt gagctgtatt taa
//
LOCUS       DAWHXS010000021        22628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWHXS010000021 DAWHXS010000000
VERSION     DAWHXS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.021"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(111..539)
                     /locus_tag="WIE22_000624"
     CDS             complement(111..539)
                     /locus_tag="WIE22_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ4126759.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(604..2352)
                     /locus_tag="WIE22_000625"
     CDS             complement(604..2352)
                     /locus_tag="WIE22_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ4126760.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2679..4112
                     /gene="ccoN"
                     /locus_tag="WIE22_000626"
     CDS             2679..4112
                     /gene="ccoN"
                     /locus_tag="WIE22_000626"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ4126761.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4139..4750
                     /gene="ccoO"
                     /locus_tag="WIE22_000627"
     CDS             4139..4750
                     /gene="ccoO"
                     /locus_tag="WIE22_000627"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ4126762.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4755..4925
                     /locus_tag="WIE22_000628"
     CDS             4755..4925
                     /locus_tag="WIE22_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ4126763.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4949..6046
                     /gene="ccoP"
                     /locus_tag="WIE22_000629"
     CDS             4949..6046
                     /gene="ccoP"
                     /locus_tag="WIE22_000629"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ4126764.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6086..6280
                     /locus_tag="WIE22_000630"
     CDS             6086..6280
                     /locus_tag="WIE22_000630"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ4126765.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6482..7891
                     /locus_tag="WIE22_000631"
     CDS             6482..7891
                     /locus_tag="WIE22_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ4126766.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7993..11202
                     /gene="recC"
                     /locus_tag="WIE22_000632"
     CDS             7993..11202
                     /gene="recC"
                     /locus_tag="WIE22_000632"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ4126767.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEYCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFESQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKKEEAGEA"
     gene            complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WIE22_000633"
     CDS             complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WIE22_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ4126768.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13172..13384
                     /locus_tag="WIE22_000634"
     CDS             13172..13384
                     /locus_tag="WIE22_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126769.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(13663..14295)
                     /gene="mtrR"
                     /locus_tag="WIE22_000635"
     CDS             complement(13663..14295)
                     /gene="mtrR"
                     /locus_tag="WIE22_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ4126770.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
     gene            14699..15937
                     /gene="mtrC"
                     /locus_tag="WIE22_000636"
     CDS             14699..15937
                     /gene="mtrC"
                     /locus_tag="WIE22_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ4126771.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWASSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            15949..19152
                     /gene="mtrD"
                     /locus_tag="WIE22_000637"
     CDS             15949..19152
                     /gene="mtrD"
                     /locus_tag="WIE22_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ4126772.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            19207..20610
                     /gene="mtrE"
                     /locus_tag="WIE22_000638"
     CDS             19207..20610
                     /gene="mtrE"
                     /locus_tag="WIE22_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ4126773.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWL
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(20802..21038)
                     /locus_tag="WIE22_000639"
     CDS             complement(20802..21038)
                     /locus_tag="WIE22_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4126774.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
     gene            complement(20978..21277)
                     /locus_tag="WIE22_000640"
     CDS             complement(20978..21277)
                     /locus_tag="WIE22_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ4126775.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFLFAVLTVLKLKGYQAGLYTLLIALAVAAFGFGMPAGMAVSSLPPQPH"
     gene            21812..22591
                     /locus_tag="WIE22_000641"
     CDS             21812..22591
                     /locus_tag="WIE22_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ4126776.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT         5499 a         5514 c         6126 g         5489 t
ORIGIN      
        1 cactgtaaaa acggagaagg acagaggacg gcagtaaggt ttgccgtcct ctttttcaag
       61 ttgcggtgga atgccgcttg aaggtgtggt ttaaacgttt cggcttgtaa tcagacgcgg
      121 gcgttgtcca gccgctctct gaagttttcc aaatttacgc ctgccgcttg ggcgcggcgg
      181 atgatttcgt caaccggcat accttctgcc gcccggcgga agccccacag gagggagcag
      241 cgcgtaccgg tccggcaata ggcaaggacg ggatattcgg cttgtccgat gagttggcgg
      301 aaggtttcga catcgtgttt ttggatgtcg cgtgcggtaa cgggttggtg atggaatcca
      361 gtaacgcctg cttgttccag ccactgtttg atttgggcga agtcgggttg cgattcttct
      421 tcgcggtcgg ggcggttgca gatgacggtt ttgatgccca gttgcgcgat ttgttccgca
      481 tcggctttgg tcagttgcgg agaaatatag aggtgttcgt caagtttcag aatggccatt
      541 ttgtttcctt tactttgcgg ttgtttttca gacggcattt tgtgtgcaat gccgtctgaa
      601 agtttaaccg aacaaccagt tgtccaacag gttccggtct gtgatatgta ggatggcagt
      661 atccgctgcc gctgcctgaa ccgtcaagac ggtttgacgc aatatgccgg cacggaagaa
      721 gtggatattg atttttgcat tgacgtggta tcgggcccat tgtgcggtaa agtcggtgca
      781 ggcataaccg tctaaagcaa tgattttgtc ttgcgggcac agtgccgcag attccgcgct
      841 gccgccgttg aagacatggg tcaggacgat gtggtcggtg ttttgtttga aacgtgcgcc
      901 aaaatcgcct gccgacggga cggggcagat gtgttctgcg tatccgccgc cgtgttgtcg
      961 gggaagcggc aggaaggtca gtcccacgcc tgcggttgcc aggcattcgg caagcggcaa
     1021 atcttcggta ctgtataacg ctttttggaa aaaatcttcc aaatccaagc cggtaatttc
     1081 ctgacagcgg atttgccagt gtttttccgg aatacccgag tgtgtgtccc tccactcccg
     1141 atagagtttg tccattaacg tatcgagaga atgtctgccg ttgcttcggt tgcgtattat
     1201 cagatcaagg cacaatgcgg caagcgcgcc tttctggtag tagctgacga tggcgttggg
     1261 gctgttttca tccggtttgt aaaatttgtt ccacgcggta aaactcgatt ccgccaaggt
     1321 ctgcctcaaa cggccgcggg tttgttgtac gcgcgtaatg ccttgtgcca gcaggtttaa
     1381 ataagattcg ggcgagatgg tgcggctgcg tgccaaaaac aaatcgtcgt aataggatgt
     1441 aataccttcg aatgcccata gttgttcggt atagttttct ttgtcgaggt cataagggac
     1501 gaacgcggca ggtttgatgg atttgacgtt ccacgcgtga aaatattcgt gggagaaaag
     1561 tccgagcaat gtggtgtagg tatcgtcggc atcggtcata ccgtacggcg gaaggctgtg
     1621 gcggtcggcg agcagggcgg tgctgtcggt gtgttccaaa ccgccgtaaa tatggtcgcc
     1681 gacgtggagc aggaacaaat atttttgaaa cggggcaggg gaggaaaaca ccgccagttc
     1741 tgtttcgcag atttttttga tatccgaaac cagcctgttg cggtcgaaat cgggatatat
     1801 gccgcttaag gcaattgtgt gcggaatgcc tgccgcctca aaatctaaaa attcaatcaa
     1861 gcccatctcg acaggtcggt caatcaattc ggcataagat gccgcctgaa agacaaacct
     1921 ccctgtttcc ggcagcgttg tggcaatacg ccattcggac ggaatacccg tcaattccaa
     1981 gcggtgcggc agcgtttccg ttccttcgac tttcaaaaac aggcacgatc cgtcaaaaaa
     2041 accgcgttct gtcgtcagga aagaacctcg aaccgacaaa tcgaatgcat atacggtgta
     2101 gcggatttgc cactcgccgc gtacggcggc ggcatgccag cggtttttgg caatttgttc
     2161 gagcggcatg gacgtgccgt tacaggatgc atggatagaa gtgatgtggc gggaaaaatc
     2221 ccgaatcaga tagctgcccg gaacccagtt tggcaggctt atttctatag gaagatcatc
     2281 atcttgtgtg aatgtcagca ggatgtgcca ctcgtggtcg agaggggacg gggttatttt
     2341 ataatgaatc ataactatca aatatatcgt taaacgggat ggattaaata cctttaaaat
     2401 gcagcttaac ctacatcaat aaagtgccac aaaaagggaa aattggaaac aaaagcaatg
     2461 gcggtcgggt tgtaatttat tataacaata tgatttatat gtaataaaaa acagattaag
     2521 caaagggctc gtcggcggcg cgaatgtaat tccgcttggc gtaataaggc agatatgtta
     2581 aatttagaaa aaattacgct tgcccggatt gcaccttaaa caggctgcgg aagcggcagg
     2641 ataaaaatct ttattcccat aaattcatgg agactctcat ggacacacaa acttacaact
     2701 acaaagtggt gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggtatgttgg
     2761 tcggcgttat cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac
     2821 cttggttcca cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg
     2881 gcggttgcgg cctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc
     2941 ttttcggcgg ttggctgccg gcatttacct tttggggctg gcaggcggta atcgttgccg
     3001 ccgtcgtcag cttccctatg ggttggaccc aaggtaagga atatgccgaa ctggaatggc
     3061 cgatcgatat tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga
     3121 ttgccaaacg taagattaaa catatttacg ttgccaactg gttctacggc ggctttattt
     3181 tggcggtcgc acttttacac atcgtcaaca atatcagcat cccggccggt ttgatgaagt
     3241 cataccccgt ctattccggt gcgattgatg ctatggttca atggtggtac gggcataatg
     3301 ccgtgggctt cttcctgact gccggcttct tgggtatgat gtactatttc gtacccaaac
     3361 aagcagcccg ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt
     3421 tcacctatat gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc
     3481 aatctttggg tatggttctg tctttgattc tgttcgcacc ctcttggggc ggtatgatta
     3541 acggcatcat gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat
     3601 tcctgattgt atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga
     3661 ttaaaacggt caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg
     3721 gtgcgttggg ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc
     3781 tgttcggcaa agaacagatg cacagcacca agctggtaga agcacatttt tggattgcga
     3841 ccatcggcgt ggttctgtat atcgctgcca tgtggattgc cggtgtgatg cagggtttga
     3901 tgtggagttc tttgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac
     3961 gcaccatgcc ttactacgtg atccgttttg caggtggttt attgtatctg agtggtatgt
     4021 gcattatggc gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa
     4081 tccctgcggt ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat
     4141 gaaattacaa caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt
     4201 agtcagtgtc ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca
     4261 gccggcaccg ggcgtgaagc cttacaatgc cttgcaggtt gccggacgcg atatttacat
     4321 ccgtgagggc tgttacaact gccactctca aatgattcgt ccgttccgtg cggaaaccga
     4381 gcgttacggt cattactctg ttgccggaga gtcggtttac gaccatcctt tccaatgggg
     4441 ttccaaacgt accggtcctg atttggcacg tgtaggcggc cgctattccg acgaatggca
     4501 ccgtatccac ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc
     4561 atggcttgca cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg
     4621 taaagtaggt actccttaca gtgatgagga aattgcgaaa gcgcctgagg ctttggcaaa
     4681 caaatccgag ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa
     4741 cgtaaggtaa catcatggat attaacggta ttcgcgctct tttcacggta tggatcttta
     4801 tctgtttcct gttggtactc tatatcgtct tcaacaggcg gaataagaaa aactacgata
     4861 atgccgcaaa cagcattttt gatgaaaacc aagatgcgca agataagaaa agcgaaaacc
     4921 gttaatattg tgataacgga gcaaaacaat gaacacaaca tcccaattta ccagtaattt
     4981 ctggaatata tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct
     5041 gctgtctcaa aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca
     5101 tgagtgggac ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg
     5161 tgttttgacg tggctgttcg gtatcggtta tttggttatg tatccgggtg tcggcgacta
     5221 caaaggtctg ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga
     5281 tgagcaatac ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa
     5341 agaccctcaa gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca
     5401 cggctctgat gctaaaggct ctaaaggttt tccgaatctg accgatagcg attggttgtg
     5461 gggcggtgat cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc
     5521 tgcctggggt cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc
     5581 cctttcaaaa cccgaaggtc agtatgatga ggaacgtgcc gcgcgcggac aagccttgtt
     5641 cagcggtccg cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg
     5701 tttgggtccg aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat
     5761 cgaaaccatt accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa
     5821 agacaaactg catattatga ctgcttatgt atggggtctt tccgataaag acggtaaagc
     5881 tccggtgaaa aaagccgagc ctgcaccgac tcccgcaccg gcggcagaac ccgcagcctc
     5941 tgctcctgca gaagcagcac aagccgcgtc cgaagccaaa cctgccgcag cagaacctaa
     6001 agccgaggaa aaagctgcac ctgctgccaa agcggacggc aaataggttt atgaaaccgt
     6061 ttgtgccgcc tgccatggca atgcgattcc gggtattccc catgtaggca tcaaagccga
     6121 ttgggccgac cgcatcaaaa aaggcaagga cacgttgcac aaacacgcga tcgaaggttt
     6181 caatacgatg cccgcaaaag gcggtcgcgg cgatttgagc gatgatgagg ttaaagctgc
     6241 ggttgactat atggtcaacc agtcaggcgg caaattctaa cttgactgag tttccgatat
     6301 tatcggttat gaaaatccag attcagtatt gaatctggat tttttgtttt aacgggcatc
     6361 cggttttaaa cgcagactgc ctatttgttg taatgaattt tgactttagt cataaagaat
     6421 tagtctttat tgtttataat cgcgcatcat cacgatagcc ctattttcaa aaggacgatt
     6481 aatggataca caaatcaaaa ctgaagccga caatcagagc aaccggcgtt atctgaccgt
     6541 ttggcggtgg catttttatg ccggtctgct ggttatgcct tttcttaccc tgcttgccgt
     6601 tacgggtttg ggtatgctgc tgtttgccaa tattaccggt aaggagggcg agcggattca
     6661 tgttgtgccg caggcaacgg tacaacctct gtctgttcag gcggaagcgg cacgcagtgc
     6721 cgttaatccg gaaacttcgt ccgtcgttca gtatattgcg ccgcgtgccg atgatatggt
     6781 tgccgtgttc cgtgtcaaca atgagggcaa ggcaacgatg gtcgcggtcg atccttatac
     6841 ggcaaaagtg gtcagtacca tgccgcgcaa tcagggttgg tattacacga tggatgaaat
     6901 ccacagcgat atgatgctcg gtgcggcagg cgattatctt ttggaaacgg cagcatcgct
     6961 gacgattatc atgataatca gcggcttgta cctttggtgg gtgaaacggc gcggcatcaa
     7021 ggcgatgctg ctgccgtcaa aaggcagggc gcgttcttgg tggcggaatc tgcacggcac
     7081 gtttggaact tgggtgtcgt tgattttgct gttgttctgc ctgtcgggta ttgcttgggc
     7141 gggtatttgg ggcggcaagt tcgtacaggc ttggagtcag ttccctgccg gtaaatgggg
     7201 tgtcgaaccg aaccccgttt cagtcgtgcc gacccacggc gaggtattga atgacggcaa
     7261 ggttaaggaa gtgccgtggg ttttggagct tacgcctatg cctgtttcag ggacgactgt
     7321 gggcaaagac ggcattaacc ctgacgagcc gatgacattg gaaaccgtcg accgctttgc
     7381 gcgggaaatc ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg aggacggcgt
     7441 atggactttg tcgcaggatt ctatgagtta cgacatgatc agcccgtttg ccgaccgcac
     7501 ggtacatatc gaccagtaca gcggcaaaat ccttgccgac atccgttttg acgattacaa
     7561 cccgttcggc aaatttatgg cggcaagcat tgcgctgcat atggggactc tgggctggtg
     7621 gagcgtgttg gcgaacgtct tgttctgcct tgccgtcatt tttatcggta tcagcggctg
     7681 cgtgatgtgg tggaaacgcc gtccgaccgg agcggtgggc atcgttccgc cggcgcagaa
     7741 agtcaagctg ccggtttggt ggatgatggc attgccgcta ttggcaatcg cactgctctt
     7801 cccgaccgca ctgcttgcca ttgccgtgat ttggctgttg gataccttgc tgctgtcgcg
     7861 gattcctgtt ttgaggagat ggtttaaatg acccgatgcc gtctgaagcg ttcagacggc
     7921 attttgtttg aaggcgggcg ggggaaaggc tatataatcc cgaatacttg accacagctt
     7981 ctgtttgaaa tcatgtttta tctgtatcaa tccaaccgtc ttgaaacgct ggcggcactg
     8041 tttgcccgca ttcaaaaagt caaaccgctg aaatcggttt tacagcccga acagattatt
     8101 gtgcagagtc aggggatgcg ccgctacctc aatacctgcc tcgcccgcga tttgggcgtg
     8161 gcggcgaatt tgtcgttcag cctgcccgcc ggtctgacgt ggaagctgat gaaaaaactg
     8221 attcccggta ttccggaact cagcccgttt gcgcctgaag tgatgcgctg gcggctgttg
     8281 gatttgttcc gcagcgaggc atttcggaat acggcagaat ttgaagatgt gaggaatgtg
     8341 ctgcaagact atctgggcag cggcgaatcg gcagattatc agcttgcggg acagcttgcg
     8401 gacatattcg accaatacct cgtgtaccgt cctcagtgga tagacgcttg gcagcagggc
     8461 aggcggctcg gtttgggcga cgacgaaatc tggcagtcca aactgtggcg ttacctcgac
     8521 gatggcaggc agagcgcgcc gcaccgtgtc gcgttgtggg aaaagctgtt ggaatcttta
     8581 agcagtgata agctgcccga gcgttatttc gtgttcggca tttccacgat ggcaccgatg
     8641 tatttgcagc ttttgcacaa gctgtccgaa tattgcgatg tgtttgtgtt cgcactcaat
     8701 ccgagcggga tgtattgggg caatgtcatc gaagcggcgc aaatcctcaa aggcggcggc
     8761 gatcccgatt taactcaggc agggcatccg ctgctcgcct cattgggcaa gcaggggcgg
     8821 gactttttcg actttttgaa cgaaatggaa atagaaggag aaacgccggt atttgaagaa
     8881 ggcgggcgcg atacgctttt gcacgccctg caaaccgata ttcaaaacct gaaaatgccg
     8941 tctgaaaatg tgggaagcgt caacacgggc gacggctcga tacgcatcgt ctccgcacac
     9001 agccccttgc gcgaattgca gatactcaaa gacaagctgt tgaaaattct gcatgaacat
     9061 cccgattggc agccgcatga tatcgccgta ttaaccccga acatcgaatc ctatacgcct
     9121 tttatcgaag ccgtgttcgg acaggcgcag cctggtgcgc aggcattgcc gtattccgtt
     9181 tcagacgtga aaatcagccg ccgacaaccg ctgtttcatg ctttgtcatg cctgttcgac
     9241 ttgttggaaa gccgatttga agtcgataaa gtgcttgtgc ttttagaaac cgcacccgtg
     9301 ttgcgccgtt tcggactgac tgaggacgat ttgccgcttt tgcacgatat ggttgccgat
     9361 ttgaacgttc actggggttt ggacggagaa atgcgcggcg gcacggatca gctgtttacc
     9421 tggaagcagg cggtagaacg catgatattg ggctggatgc tgcccgaagg cggcaacccg
     9481 atgtggcagg atgtcagcgc gtggtatgcc gacgtgaatc aaaccgccct gttcggacgt
     9541 tttgccgccc tcctcgaaac cctgtcggat attgtacgga tatggcggca gcccgcaacg
     9601 atcggcgaat gggtggcgcg ttgccgggat ttgcttgaaa cattgttcca agctgaagcc
     9661 gatgaccaaa agtcagtcca aaaccttgaa aacgaatggg tcaaatggca ggcggaaaca
     9721 gaattggcgc aattttccgg acagttgccg caacaaaccg tcatccgcca tatccgacgt
     9781 ttcctcgaca gcgaaagcga ggcaggcttt ttacgcggcg gcatcacctt ttgcagtatg
     9841 gtgccgatgc ggagcctgcc gttcaaagtc atctgcctgt tgggtttgaa cgacggagat
     9901 tttccccgca ataccaaagc cgccgtattc gacctgattg ccaaacatcc cgccaaaggc
     9961 gaccgcgccc gccgcgatga cgaccgctac ctcttcctcg aagccctcat cagcgcgcgc
    10021 gaaatcctct acctgtccta catcgggcgc gacatccgca aagacgaaga gcttgctccg
    10081 tcttcgctgt tgggcgaact catcgacaca gttgccgcta tggcgggcat cggtagccgc
    10141 caacttgcac aaaactggat agaacagcat ccgctgcaag ccttctcgcg ccgatatttt
    10201 caagaaggcg gacgttcaga cggcatattc ggcacgcgta ccgactacgc cactgcgctc
    10261 ggacaaacgc ccgaaccgcc gcaacccttt ttcgaccaac ccgtagaaaa cgccgaacct
    10321 gttgccgaaa tcggacagga cgaatttatc cgtttctggc gcaaccccgt caaagtatgg
    10381 cttcagcagc agcttgcgtg gagcgaaccc catatcggcg aagcctggga gcctgccgaa
    10441 cccttcgagt cgcaacacgc cgatcaaatc gccgaaacct acatcgaagc acggcgcgaa
    10501 ggacgggatt ttgcccaaac cgccgcccgc atcggggcgg aaagcctcct gccgtcggga
    10561 gagttgggca gactttggca gcagaacttc caaactgctg ccaaacaaat cgacacggcg
    10621 gttttaaaca gccccaaact gccaccgctt tcatatgcca taccgtcgga cgggcaaatc
    10681 ctgaaaggca gcttgggcaa tctgtaccgc tgcggacaag tgttttacgc ctacggcaaa
    10741 cccaacgcgc cgcaacgtat cgcttttctg ctggaacacc tgatattttg cgccgttatg
    10801 ccgtctgaag ccgaaacgcg gcaaaccttt atcgtccaat ccggagaaac cgaaatattg
    10861 gcggaaatcg cgcaagacag ggcattgcag ctattgtcgg aatggatggc gtttttcaat
    10921 atcgggcaaa accgcccgct gccgtttttt gccaagacct cgcttgccgc tgccgaagcg
    10981 tttgcccaaa aacaagattg ggaagccgcc ctgaaaaaag cccaaaccgc ctattacggc
    11041 aacaaagtca gcaaagggca gaaagactat accgaagtcg ccctcgtgtt cggcaacgca
    11101 agccaaaacc cactcgaaca acccctgttt gaaaacctcg cccgcctgct tgccgacacg
    11161 cttgccgcag cggaaaaaaa ggaagaggcc ggagaagcct gacgggatag aaaggcaaat
    11221 gccgtctgaa agcgtttaag catttcagac ggcattttgt ttatcgtgtt taaggtgcgg
    11281 gatagaatgt gggcgcgccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga
    11341 ataaggcaat ccatgcaatt aagaagaaag cggaatacgg caacatcata gaaatcagcg
    11401 tacccacgcc cgcatctttt ttgtatttga tcaccgtcgc cataatcagc ccgaaataac
    11461 tcatcatcgg cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga
    11521 cttcgggcgc gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc
    11581 attgcgcgga ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga
    11641 taaacaacac gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa
    11701 tatattgccc aatattcgtc caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga
    11761 tgaccaaata aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt
    11821 cgccgcgcaa acttcgggtt acccggccat aaacaatgcc cggcagcgca aacaacaaga
    11881 aaataaaaac aacaatcgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat
    11941 gacgcaaaat accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa
    12001 acaccacgcc agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgcc
    12061 gaatgtcttt ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga
    12121 ttttttcagt aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa
    12181 accagttggc ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg
    12241 tgatgcctgc caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg
    12301 aaacgccggc gaaagccgca gccagaccgg caagcggatg gcggccgagg gaatgaaaga
    12361 tgatggcgga caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa
    12421 tccctgtaaa aacaaccata aaagtagtga gtttgcgtgg agattttgtg agcaataagc
    12481 gcattaatgc ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca
    12541 acaccgttcc caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga
    12601 tcaaaccgtc agcatcgagc aggctgacaa cgtgaatcaa accgtcatcg gcacgtcctt
    12661 tcgcaccaac agggcgcgga tcggggacgg atagtccgaa atacgcaccg gcggcagagg
    12721 caatcagcaa taacacaatg aaaataataa aaagcgtaac cgggtgcggc aacatattgc
    12781 ccagccattc gactgtgcgt aaaaatcgtc cgtcccgttg cgtatcggtt tgactcatct
    12841 gcttctcctt aaaaaaaata tattgagtgc attaaatttt tgaatgtaac atgaagtgtt
    12901 ttgtaagcaa actaaaataa tcgtatatgt atataaccgt aatagattaa aacagaaaat
    12961 gcccacatcg ttattttcat aaaggcggta atccgaaaac ttgacgttaa agccttatca
    13021 ggaatgactg gaactcaaaa aaacggattc ccgcctgcgc gggaatgacg ggattttcgg
    13081 tttttgtttg caagaatgcc ggagaagaat gcagtttaca ttttgaatga tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaatcatgga
    13201 caaatataat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt
    13261 atcgctgatg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatatcca tgcgatgata ggggaaatta aaaatgcaat
    13381 ttagtgaata tgagaccttt gcaaaaatag tctgttaacg aaatttgacg cataaaaatg
    13441 cgccaaaaaa ttttcgattg cctaaaacct tcctaatatt gagcaaaaag taggaaaaat
    13501 cagaaaagtt ttgcattttg aaaatgagat tgagcataaa attttagtaa cctatgttat
    13561 tgcaaaggtc tcaatatgta tttttagaag aaggctttgt gtcattttga tgccgtctga
    13621 aagggtttgt tcgtttcaga cggcattgct accaaggctt gattatttcc ggcgcaggtc
    13681 gggatggttt tccaagttgt ccatcattat cccgatgatg cgcggggcgg ttttgcccaa
    13741 atcgaaacgt tcgcaggaag agaaccaccg ccaaatcagc ccgtccaagg ttgatttgat
    13801 gaagataact gccgtttcct tgtccaaatc gtcagccaaa tcctgatttt ccaccgcttc
    13861 ggtcaaaacg gcggtaattt tctcgcgcca gattgcctga tgcttgcggg caatggcgat
    13921 aacggcggcg ttttgctccg tgtgttcgca ttttaaaaac aggatgttgt ggaatttgta
    13981 gtagatgtcg ttgctttgca gccgctcgaa aaagtgcagc agcgtgtggc ggaataccgc
    14041 ccaagaccct ccttcggcat cttcggcatc ttgcgcgatg cagttttcga tgtcgtcgca
    14101 gatacgttgg aacagcgcgt cgaacaagtc ttccttattt ttgaaatgcc aatagagcgc
    14161 gccgcgcgtt acgccggcgg cttgggcgat ttcgttgagc gaggtgcgcg caatcccttt
    14221 gcggtaaaag gtttccaagg cggcaagcat caggtgttct ttggttttca aggcttcggt
    14281 tttggttttt ctcataatgg cgttttcgtt tcgggtcggt ttgatgaggg cggattataa
    14341 aaaagacttt gtaaccatgc aatcgtgtat gtataatgaa acccatgaaa ttgagactac
    14401 atctcaactt tgaaaacagg cataacctgc tttgcaaccc gttcgaacat tcgggtatca
    14461 aagcaggcgg tttttatatc ctgaaatata gtggattaac aaaaatcagg acaaggcgac
    14521 gaagccgcag acagtacaaa tagtacggaa ccgattcact tagtgcttca gcaccttaga
    14581 gaatcgttct ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat
    14641 aagaatgcct gttgattggc aaaacccttt acatcaaacc agacagatag gataaataat
    14701 ggctttttat gcttttaagg cgatgcgtgc ggccgcgttg gctgccgccg ttgcattggt
    14761 actgtcgtct tgcggtaaag gcggagacgc ggcgcagggc gggcagcctg ctggtcggga
    14821 agcccctgcg cccgtcgtcg gtgtcgtaac cgtccatccg caaaccgtcg cattgaccgt
    14881 cgagttgccg gggcgtttgg aatcgctgcg taccgccgat gtccgcgccc aagtcggcgg
    14941 catcatccaa aaacgcctgt tccaagaagg cagttatgtc cgtgccggac agccgctgta
    15001 tcagatcgac agttccactt atgaagcagg tctggaaagt gcgcgcgcgc aactggcaac
    15061 ggctcaggca acgcttgcca aagcggatgc ggatttggcg cgatacaagc ctttggttgc
    15121 cgccgaagcc gtcagccggc aggaatacga tgctgcggta acggcgaaac gttctgccga
    15181 ggcaggtgtc aaagcagcac aggcggcaat caaatctgcc ggcattaatc tgaaccgttc
    15241 gcgcattacc gcgccgattt ccggctttat cggtcagtcc aaagtttccg aaggtacgct
    15301 gttgaatgcg ggcgatacga ccgtgctggc aaccatccgc caaaccaatc cgatgtatgt
    15361 gaacgttacc cagtctgcat ccgaagtgat gaaattgcgc cgtcagatag ccgaaggcaa
    15421 actgctggcg gcggatggtg tgattgcggt cggcatcaaa tttgacgacg gtacagttta
    15481 ccctgaaaaa ggccgcctgc tgtttgccga tccgaccgtc aacgaatcga ccggtcagat
    15541 taccctgcgc gccgccgtac cgaacgatca gaatatcctg atgcccggcc tgtatgtgcg
    15601 cgtgctgatg gaccaagtgg cggtggataa cgcatttgtt gtgccgcagc aggcggtaac
    15661 gcgcggtgcg aaagataccg tgatgattgt gaatgcccaa ggcggtatgg aaccccgcga
    15721 ggtaacggtt gcgcaacagc agggtacgaa ttggattgtt acgtcgggtc tgaaggacgg
    15781 ggacaaggtg gttgtggaag gcatcagtat cgccggtata acgggtgcga aaaaggtaac
    15841 gcccaaagaa tgggcgtcgt ctgaaaacca agccgccgcg cctcaatccg gcgttcagac
    15901 ggcatctgaa gccaaaactg cttctgaagc ggaataagga aggcatcgat ggctaaattt
    15961 tttatcgacc gccctatttt tgcgtgggtt atttcgattt tcattattgc ggcgggtatt
    16021 ttcggcatca aaagcctgcc tgtttcgcaa tatccgtctg tcgccgcccc gaccattact
    16081 ttgcacgcca cttatccggg tgcgtccgcg caggtaatgg aagacagcgt gctttccgtg
    16141 atcgagcgga atatgaacgg cgtggaaggt ttggattata tgtccacttc cgccgattcg
    16201 agcggcagcg gcagcgtgag cctgaccttt acgcccgata ccgacgagaa tctggcgcag
    16261 gtggaagtgc agaacaagct ttccgaagta ttgagcacgc tgccggcaac cgtccagcaa
    16321 tacggcgtaa ccgtatccaa ggcgcgttcc aatttcctga tgattgtgat gctttcgtcg
    16381 gatgtgcagt caaccgaaga gatgaacgac tacgcgcagc gtaatatcgt tcccgaactg
    16441 cagcgtatcg aaggcgtggg gcaggtacgc ctgttcggcg cgcaacgcgc gatgcggatt
    16501 tgggttgatc ctaagaaact gcaaaactac aatttgtcgt ttgccgatat cggcagtgcg
    16561 ctgtccgccc agaacgtcca gatttcagcg ggttctatcg gttcgcttcc cgccgttcgc
    16621 ggacagacgg ttacggctac cgtaacggcg caagggcagt tgggtacggc agaagaattc
    16681 ggcaacgtca tcctccgcgc caataccgac ggttctaatg tttacctgaa ggatgtggca
    16741 agggtcggac tgggtatgga agactattct tcctcaaccc gtctgaacgg tgtaaatacc
    16801 accggtatgg cggtgatgct gtccaacagc ggcaatgcga tggcgacggc aaaggcggtt
    16861 aaagaacgca tggcgacgtt ggaaaaatac tttcctcagg gtatgagctg gaaaacccct
    16921 tacgatactt ccaaattcgt cgaaatttcg attgaaaaag tgattcatac tttaatcgaa
    16981 gcgatggtgc tggtgtttgt cgtaatgtat ctcttcctgc aaaacatccg ttatacgctg
    17041 attccgacca tcgtcgtacc gatttcgctg ttgggcggtt tcgctttcat ctcttatatg
    17101 ggcatgtcga ttaacgtact gaccatgttt gcgatggttt tggtcatcgg catcgtggtc
    17161 gatgacgcga ttgtggtggt tgaaaacgtc gagcgcatta tggcgggtga aggcttgccg
    17221 cccaaagaag cgaccaaaaa agcgatgggt cagatttcgg gcgcggtcat cggtattacc
    17281 gccgttctga tttccgtgtt cgtaccgttg gcgatgttca gcggggcgac gggcaatatt
    17341 tacaaacagt ttgccctgac gatggcgtca tcaatcgcat tctccgcctt ccttgcgctg
    17401 acccttaccc ctgctttgtg tgccacaatg ttgaagacaa tcccgaaagg gcatcacgaa
    17461 gagaaaaaag gtttcttcgg ctggtttaac aagaaattca acagttggac gcacggttac
    17521 gaaggccggg ttgccaaagt gctgcgtaag actttccgca tgatggttgt ctatatcggc
    17581 ttggcggttg tgggcgtgtt cctgtttatg cgcctgccga cttcgttcct gccgaccgaa
    17641 gaccaaggct tcgtcatggt cagcgtgcaa ctgcctgcag gagcgaccca agagcgcacc
    17701 aatgcgactt tggcgcaagt tacccaactg gcgaaaagca ttcctgaaat agaaaacatc
    17761 attaccgttt ccggcttcag cttttcgggc agcggtcaga atatggcgat gggttttgcc
    17821 atattgaaag attggaacga gcgtaccgcg cccggcagcg atgccgttgc gattgccggc
    17881 aagctgacgg gtatgatgat ggggacgctt aaagacggtt ttggcatcgc cgtcgtcccg
    17941 cctccgattc tggagttggg caacggttcg ggtctgagca tcaacctgca agaccgcaac
    18001 aataccggcc ataccgcatt gctggcgaag cgcaacgagt tgattcagaa aatgcgtgcc
    18061 agcggtttgt ttgaccccag caccgtccgt gccggcggtt tggaagactc gccgcagttg
    18121 aaaatcgaca tcaaccgtgc cgccgcggcg gcgcaaggca tttcgtttgc cgacatccgc
    18181 accgcattgg caagtgcgct gagttcgtct tatgtcagcg acttcccgaa ccaaggccgt
    18241 ctgcaacgcg tgatggtgca ggccgacgag gatgcccgta tgcagcctgc cgatattttg
    18301 aacctgaccg tgccgaacaa atccggcgtc gccgtaccgc tttccaccat tgctactgtt
    18361 tcttgggaaa acggtacgga acagagcgta cgcttcaacg gttatccttc gatgaagctg
    18421 tccgcttcgc ctgcaaccgg cgtttccacc gggcaggcta tggcggcggt tcaaaaaatg
    18481 gttgacgaat tgggcggcgg ttacagcctg gagtggggcg gacagtcgcg cgaagaggca
    18541 aaaggcggct cgcaaaccct gattttgtac ggtttggcgg ttgcagccgt atttttggta
    18601 cttgccgcgc tttatgaaag ctggtcgatt ccgctggcgg tcatccttgt gattccgttg
    18661 ggtttgatcg gtgcggctgc gggcgtaacc gggcgcaacc tgtttgaagg actgttgggc
    18721 agcgttccct cgttcgccaa cgacatctac tttcaagtcg gtttcgttac cgtgatgggt
    18781 ttgagtgcga aaaacgcgat tttgattatc gagtttgcca aagaccttca agcgcaaggg
    18841 aaaagcgcgg ttgaagccgc tttggaagca gcccgcctgc gtttccgtcc gattatcatg
    18901 acctcgtttg cctttatttt gggcgtggtt cccctgtata ttgccggcgg tgcaagttct
    18961 gccagccagc gcgccattgg tacaaccgta ttctggggga tgctcatcgg cacgctcttg
    19021 tcggtgttcc ttgttccgct tttctatgtg gtggtgcgca aattcttcaa agaaaccgcg
    19081 cacgaacacg aaatggcagt aaaacacgcc gccgaagcgg gcatcaccgg ttcggacgac
    19141 agccaacatt aagcaaccat gccgtctgaa cgcccacggg ttttcagacg gcatcaggac
    19201 ttttttatgg atactacatt gaaaaccacc ttgacttctg ttgcagcagc cttcgcatta
    19261 tccgcctgca ccatgattcc ccaatacgag cagcccaaag tcgaagttgc cgaaacgttc
    19321 aaaaacgata ccgccgacag cggcatccgc gccgtcgatt tgggttggca cgactatttt
    19381 gccgacccgc gcctgcaaaa gctgatcgac atcgcactcg agcgcaatac cagtttgcgt
    19441 accgccgtat tgaacagcga aatctaccgc aaacaataca tgattgagcg caacaacctc
    19501 ctgcccacgc ttgccgccaa tgcgaacgac tcgcgccaag gcagcttgag cggcggcaat
    19561 gtaagcagca gctacaaagt cggactgggt gcggcatctt acgaactcga tctgttcggg
    19621 cgtgtacgca gcagcagcga ggcggcactg caaggctatt tcgccagcac cgccaaccgc
    19681 gatgcggcac atttgagcct gattgccacc gttgccaaag cctatttcaa cgaacgttat
    19741 gccgaagaag cgatgtcttt ggcgcaacgt gttttgaaaa cgcgcgagga aacctacaag
    19801 ctgtccgaat tacgttacaa ggcaggcgtg atttccgccg tcgccctacg tcagcaggaa
    19861 gccctaatcg aatctgccaa agccgattat gcccatgccg cgcgcagccg cgaacaggcg
    19921 cgcaatgcct tggcaaccct gattaaccaa ccgatacccg acgacctgcc cgccggtttg
    19981 ccgttggaca agcagttttt tgttgagaag ctgccggccg gtttgagttc cgaagtattg
    20041 ctcgaccgtc ccgatatccg tgctgccgaa cacgcgctca aacaggcaaa cgccaatatc
    20101 ggtgcggcac gcgccgcctt tttcccatcc atccgcctga ccggaagcgt cgatacgcat
    20161 tctgccgaat tgggcgggct gttcaaaagc ggcaccggcg tttggttgtt cgcaccttcc
    20221 attaccctgc cgatttttac ctggggtacg aacaaggcga acctcgatgt ggcaaaactg
    20281 cgccaacagg cacaaatcgt tgcctatgaa tccgccgtcc aatccgcatt tcaagacgtg
    20341 gcaaacgcat tgaccgcgcg cgagcagttg gataaagcct atgatgcttt aagcaaacaa
    20401 agccgcgcct ctaaagaagc gttgcgtttg gtcggtctgc gttacaaaca cggcgtatcc
    20461 ggcgcgcttg atttgctcga tgcggaacgc agcagctatt cggcggaagg tgcggctttg
    20521 tcggcacaac tgacccgcgc cgaaaacctt gccgatttgt acaaggcact cggcggcgga
    20581 ttgaaacggg atacccaaac cgacaaataa ccggtcgggc aataaaatgc cgtctgaaac
    20641 gataaggttt cagacggcat ttctttcttt atagtggatt aacaaaaacc agtacagcgt
    20701 tggctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca agttgtacta
    20761 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatgtttg
    20821 acggttgcgg tcaggctgcg cggcgtgccg taaacgtgaa tgtcgggcat agggcggtag
    20881 cgtttgttga acacgttttc aaagtccaaa ccgatgcggg tggatttgcc gattttgtaa
    20941 tgcgcggtca aatccagcgt ggcgtagggg cgttgggtca gtgcggctgc ggcggcaggg
    21001 aagaaaccgc catacccgcc ggcatcccga agccgaatgc ggcaacggca agcgcaatca
    21061 gcagcgtata aagccccgcc tgatagcctt tcagcttcag gacggtcagc acggcaaata
    21121 aaaagacgat gggtaagagt gcggcggcgg cagtcagata caggctgccg ccgattgccg
    21181 tgtagttttg aacccaagtt tccataattg aacatctccg aaaatatttt tctaatcgtc
    21241 ggcaatagtg gtcaaaccaa ttaaagcaac gttgcattac tttgcgaaac tttaatattc
    21301 aggtcaatag agtggattaa atttaaacca gtacggcgtt gcctcgcctt accgtactat
    21361 ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atatattttt
    21421 gggcggttcg gcagatttga atcggagctt tcgtttaaat cctgtcaaaa caaatatttg
    21481 cataaacaaa aattgtagtt tggtgtagtt ttttcctgtg tttcgggggc ggtgcgaggt
    21541 aaatgccgtt gacggcggga acggcgtgtc attagaatcc gccctgattg gtcagtccaa
    21601 tttgatgttt gatgtatagt ggatgaacaa aaaccagtac ggtgttgcct cgccttagct
    21661 caaagagaac gattctctaa ggtgctgaag caccaagtga atcggttccg tactatttgt
    21721 actgtctgcg gcttcgtcac cttgtcctga tttttgttaa tccactatat ttcggaaggg
    21781 ttctgcaggg aatctttata aaggaggagt aatgaaactg gtaaggcctc agaaaatcag
    21841 cgatcaggta ttgtcggtat tggaggagcg cattgcggaa ggggtttacg cggaaggggg
    21901 caagattccg cccgaacgcg ttttggcgga agagttcggc gtgtcccgcc cgtcggtcag
    21961 gtcggcattg aatgttttgg ttgcccgtca ggttttggaa gcgaggcagg gagacggtta
    22021 ttatgtttcg gtcaagccac agcaggattt tttgcaaagt tggcaggagc ttttgggcaa
    22081 gcattccaat tgggaacagg atgtttttga tttcagctgc cacatcgagg gctgtatggc
    22141 ggcattggcg gcagaacgcc ggacggatgc cgatttgaag cggattgggt tttggcttga
    22201 aaagtttgag gaggcgtgcg aaagcggcaa tctggaacat cagagcgaag ccgatgtcag
    22261 cttccatcag acgattgcgg atgcggcgca caatttgttg ttcagccatt tgtcgggcgg
    22321 tttgttgaaa atgctgtacc ggcagacgcg cagcagcctt atttatctga atcaggaaga
    22381 agatccgcgt ccgaaactga tggcgcagca ccgcgtgctg tatgaggcga tatcgaaccg
    22441 tcggccgggc gaggcttcgg aggcggcaaa ggcacatttg aattatgtgg caagcagcat
    22501 catgaaagac agggaatatc agagccgtaa ccgccacgcg gatactttgg cgcaaaacga
    22561 tttgaagcgc gtgcaggatt gggaggtatg aataccgttt agacggtctg gcatattcgg
    22621 atgtatcg
//
LOCUS       DAWHXS010000022        22509 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWHXS010000022 DAWHXS010000000
VERSION     DAWHXS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.022"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(225..548)
                     /locus_tag="WIE22_000642"
     CDS             complement(225..548)
                     /locus_tag="WIE22_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ4126777.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(627..1283)
                     /locus_tag="WIE22_000643"
     CDS             complement(627..1283)
                     /locus_tag="WIE22_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ4126778.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRERFVDASLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYAAALLAKLAGRV
                     VSDDLDAEQQNRAKAVLDGLGLDNIDYVQNNGLTEPSAGAPFDAVYVGGAVNMVPDVL
                     KQQLKDGGRMAVIVGHKPVQRALLITRRGDTFEEKVLFDTLAAGLTGKDAHPFDSFNF
                     "
     gene            complement(1386..1889)
                     /locus_tag="WIE22_000644"
     CDS             complement(1386..1889)
                     /locus_tag="WIE22_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ4126779.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTIVVEDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHPAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2073..2846
                     /locus_tag="WIE22_000645"
     CDS             2073..2846
                     /locus_tag="WIE22_000645"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ4126780.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPNNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2853..3203
                     /gene="secG"
                     /locus_tag="WIE22_000646"
     CDS             2853..3203
                     /gene="secG"
                     /locus_tag="WIE22_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ4126781.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3217..3302
                     /locus_tag="WIE22_000647"
     tRNA            3217..3302
                     /locus_tag="WIE22_000647"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3251..3253,aa:Leu,seq:gag)
     gene            complement(3362..3604)
                     /locus_tag="WIE22_000648"
     CDS             complement(3362..3604)
                     /locus_tag="WIE22_000648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126782.1"
                     /translation="MDGFPLIPYGASVFPASYFPLGIPDLVVVQLYFHVFFAVFSDAV
                     KNQVSSIRADWTGLYGQEGGRQDNNQPEILVLIGIG"
     gene            4103..4470
                     /locus_tag="WIE22_000649"
                     /pseudo
     CDS             4103..4470
                     /locus_tag="WIE22_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4467..4778
                     /locus_tag="WIE22_000650"
     CDS             4467..4778
                     /locus_tag="WIE22_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ4126783.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5653..6207)
                     /locus_tag="WIE22_000651"
     CDS             complement(5653..6207)
                     /locus_tag="WIE22_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126784.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRKLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYSINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(6204..7013)
                     /locus_tag="WIE22_000652"
     CDS             complement(6204..7013)
                     /locus_tag="WIE22_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ4126785.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            complement(7006..7818)
                     /locus_tag="WIE22_000653"
     CDS             complement(7006..7818)
                     /locus_tag="WIE22_000653"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ4126786.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKEGFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            complement(8053..10683)
                     /locus_tag="WIE22_000654"
     CDS             complement(8053..10683)
                     /locus_tag="WIE22_000654"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ4126787.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFKRLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEASLVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10950..11465
                     /locus_tag="WIE22_000655"
     CDS             10950..11465
                     /locus_tag="WIE22_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ4126788.1"
                     /translation="MKIKQIVKPGLAVLAAGILSACATKSNVKADGATDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVHEWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            11472..11915
                     /locus_tag="WIE22_000656"
     CDS             11472..11915
                     /locus_tag="WIE22_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ4126789.1"
                     /translation="MPFRLFLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            12084..14141
                     /gene="ppk1"
                     /locus_tag="WIE22_000657"
     CDS             12084..14141
                     /gene="ppk1"
                     /locus_tag="WIE22_000657"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ4126790.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAREGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            14166..14435
                     /locus_tag="WIE22_000658"
                     /pseudo
     CDS             14166..14435
                     /locus_tag="WIE22_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14404..15507)
                     /locus_tag="WIE22_000659"
     CDS             complement(14404..15507)
                     /locus_tag="WIE22_000659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ4126791.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(15586..17142)
                     /gene="dnaA"
                     /locus_tag="WIE22_000660"
     CDS             complement(15586..17142)
                     /gene="dnaA"
                     /locus_tag="WIE22_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ4126792.1"
                     /translation="MTLAEFWPLCLRRLHDILPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVESAVEPAEPSLHA
                     VSEEMPVQEVLLDELPSEGSAKSVQAKTAADILAERMKNLPHEPRHTAGSASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            17434..17568
                     /gene="rpmH"
                     /locus_tag="WIE22_000661"
     CDS             17434..17568
                     /gene="rpmH"
                     /locus_tag="WIE22_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ4126793.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            17571..17936
                     /locus_tag="WIE22_000662"
     CDS             17571..17936
                     /locus_tag="WIE22_000662"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ4126794.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRNWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            18001..18222
                     /gene="yidD"
                     /locus_tag="WIE22_000663"
     CDS             18001..18222
                     /gene="yidD"
                     /locus_tag="WIE22_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ4126795.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            18395..20032
                     /gene="yidC"
                     /locus_tag="WIE22_000664"
     CDS             18395..20032
                     /gene="yidC"
                     /locus_tag="WIE22_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ4126796.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(20113..20823)
                     /locus_tag="WIE22_000665"
     CDS             complement(20113..20823)
                     /locus_tag="WIE22_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ4126797.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTARA
                     HLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLKALEQR
                     SRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(20885..21475)
                     /gene="maf"
                     /locus_tag="WIE22_000666"
     CDS             complement(20885..21475)
                     /gene="maf"
                     /locus_tag="WIE22_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ4126798.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPKALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            21623..22126
                     /locus_tag="WIE22_000667"
     CDS             21623..22126
                     /locus_tag="WIE22_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ4126799.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            22160..22339
                     /gene="rpmF"
                     /locus_tag="WIE22_000668"
     CDS             22160..22339
                     /gene="rpmF"
                     /locus_tag="WIE22_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ4126800.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
BASE COUNT         5642 a         6366 c         5423 g         5078 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa actttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg gagaggggat tggggtgcag gctgccttta aggttcaggc aaattttaac
      121 ttcgttgata ccgcgcttta gcttcgcaga agctgcactt tcagacgacc ttttggaaca
      181 ccacaggtac acgcatttaa ggaatgccgt ctgaaatgcc tgcctcaata acgcatcatg
      241 ttgccgtcaa tctcggccgc ccatgcatcg atgccgccct gaaggttgta caggttttca
      301 aaacccgcgt ccgccagata catcgccgta tgcaggctgc ggataccgtg gtggcaatac
      361 accacaagcg gcacatcatc cggcagctcg ttctgccgca gcggaatcag attcatcggg
      421 atatgcagcg catttggcag cgaacaaacc gccgtttctt catccgtacg cacgtccaac
      481 aaacaaaaca tccgcccttc gtccatccac gccttcaatt ccgccgaccc aagttgcaca
      541 atatccatcg caccccccaa aaaaaaccaa gcaaaatgcc gtctgaagcc ccaaacccgc
      601 tttcagacgg catcgcaatc cgaacattaa aaattaaagc tgtcgaaagg atgggcatcc
      661 ttgccggtca aaccggccgc caaagtatcg aacagcacct tctcttcaaa cgtatcgccc
      721 ctgcgcgtaa tcaaaagcgc gcgctgcacc ggcttgtgtc cgacgataac cgccatacgc
      781 cccccgtctt tcaactgttg tttcaacacg tcaggcacca tattgaccgc gccgccgaca
      841 taaaccgcat caaaaggcgc gcccgcagaa ggttcggtca acccgttgtt ttgcacataa
      901 tcgatattgt ccaaacccaa accgtccaac accgctttgg cgcggttttg ctgttcggca
      961 tcgaggtcgt ccgacaccac cctgcccgcc aattttgcca acagcgcggc cgcatagccc
     1021 gagcccgtgc cgatttccaa aaccgtatcg tttttcgtca gcttcaaacc ttgcgccagc
     1081 cgcgccacga ctttcggctc cagcatctta tgaccgttgg caagcggcag cgccatatcc
     1141 gcatacgcca acccctgcaa agacgcatcc acgaaacgct cgcgcggaat ctcctccaaa
     1201 gcgtccaaca catcaaaatc caatacatcc cacggacgga tttgctgttc gaccatattg
     1261 aaccgcgctt tttcaaaatc catttaatgc tccgtcaaat aattgtttga acaatggcag
     1321 gcattataaa accacacttc cgccgcgcca acttcggcac gcgccgcccg cctgtttttt
     1381 cagtctcaag ccgcctgacg cgaagcctca aaccgcttct ccaaaatctt cgccagttcg
     1441 cccaaataca aagcatccgt ctcatcaaac tgcgccggat gttcgctgtc cgcgtccaac
     1501 acgccgatac agcggccgtc tgaaaacagc ggcacgacga tttccgaacg cgacaaagac
     1561 gaacaggcaa tatggtcggg atgcgcgtcc acatcctcga ccacaatcgt cccgcccttc
     1621 gcccaagcct gaccgcacac cccgcgaccg aacggaatcc gcgtacacgc caaaggcccc
     1681 tgaaacggtg ccaaaaccaa ttcgtccgaa cgcgtatcga ccaaataaaa acccacccaa
     1741 aaccagccga acgcctcctt caaaaccgcc gccgtgttcg ccaaattcgc cacccaatcc
     1801 gtctcatccg ccaccacaga ctcaatctgc ggcaacacct cccgataaag cgcggccttg
     1861 tccgaagccg aaaaatgaag cgcgtgcatc acatctccta tagttgcata catatcaggc
     1921 ggccattata aaacaacctg cccgaaacaa cattccaaac cgcccccgca gccgcccgcc
     1981 ccaagttgcg aacccgccgc atatccacta aacttcacat tgcaacgcgc cacacgcggc
     2041 agacagaaaa aacacgacac ggagcaaaaa agatgtatca ccaaatcgga atgtgggatc
     2101 aaaaatgggt catcggcaac tggaaaatga acggccggct ccagaacaac aattcgctca
     2161 tgcaccgctt ccgcatcctc cccaccgccg aacgcgtcct catcggactc gccgccccga
     2221 ccgtttacct gctgcaactg cacaacgcca tgcaaatcgt cttaaacaac cgcatcctca
     2281 cctgcgccca agacgtgagc cgcttcccca acaacggcgc gtacaccggc gaagtgtccg
     2341 ccgaaatgct cgccgacacc ggcacagaca tcgtcctcat cggacactcc gaacgcagcc
     2401 tttatttcgg cgaaaaaaac gaaatccaac gccgcaaaat ggaaaacgtc ctcaacgtcg
     2461 gactcatccc gttattgtgc gtcggcgaaa gcctcgaaga gcgcgaagcc ggcaaagaac
     2521 acgaagtcat cgcccatcag ctttccatcc tgcaagggct ggataccaaa aacatcgccg
     2581 tcgcctacga acccgtctgg gcgatcggca ccggcaaagt cgccaccgtc gaacagattg
     2641 ccgatatgca cgcattcatc tacaaagaaa tcttgtcttt gtgcggaagc gatgttaaaa
     2701 tccgcgtcct ttacggcgga agtgtgaaag cggacaacgc ggccgacatc ttcgcagtac
     2761 cttatgtgga cggcgcactc gtcggcggcg cgtcattgtc gtacgactcc tttaccgcca
     2821 tcatcagtgc cgcacaaaat gcgtagaaaa atatggaagc cttcaaaacc ctaatttgga
     2881 ttgttaatat aatttccgct ttggccgtca tcgtgttagt attgctccaa cacggcaaag
     2941 gcgcggatgc cggcgcgacc ttcggatcgg gaagcggcag cgcgcaaggc gtattcggct
     3001 ctgccggcaa cgctaacttc ctcagccgtt cgaccgccgt tgcagcaaca tttttctttg
     3061 caacctgcat ggctatggtg tatattcaca cccacacgac aaaacacggt ttggacttca
     3121 gcaacgtaca acaaactcag caagcaccca aacccgtaag caataccgaa ccttctgccc
     3181 ctgttcctca gcagcagaaa taacagtttt tcaaatgccg acatggtgaa attggtagac
     3241 acgctatctt gagggggtag tggccgtagg ctgtgcgagt tcaaatctcg ctgtcggcac
     3301 caacacacaa aacgcttaga aattctaagc gttttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggattt caggctgatt gttatcctgc cgtccccctt
     3421 cctgaccgta caatcccgtc caatccgccc taattgaact aacctgattt tttacggcat
     3481 ccgaaaatac cgcgaaaaat acgtgaaaat aaagctgaac gaccaccaaa tcaggaatgc
     3541 caagcgggaa atagcttgcg gggaatactg acgcaccgta gggaatgagg gggaaaccgt
     3601 ccaagatgca gagcggtttt ttttgggttt ttggaaaaaa cctatactat agtggattaa
     3661 atttaaactg gtacggcgtt gcctcgcctt gccgtaccgg tttaaattta attcactata
     3721 aaataagaaa aagataaaaa attggtaaca aatgcggtaa caaatggtaa cgaatcggta
     3781 acaacttttg gggttttccg gtttttcacc gtcttggcag tgggagcgta gcggaatgaa
     3841 aagccaaaac gcacggaacc gcgcctattt tgagcaggaa tggcggttaa accacttggt
     3901 tatatatggg ggcataggaa gacagcgaaa cgcgctaaaa acgcaatttg agccattaaa
     3961 agcgattgat taaaaaataa tcaggttagc cgccagtgtt tcaggcggca taaacggaga
     4021 aattgcgggg cataaaaaag gcagcttgcc gtgttgtctg tctctggtat aattccaagt
     4081 atcactaatc aacggctaca caatgcggat attcaaaaac caatggatag tgaaatttgc
     4141 caagaagcac aaaatcaacg attccgagct gctggaagcg gtagagcggg cggataacgg
     4201 gctgatagac gcagatttag gcggcggtgt gattaagcag cgcatagcaa ggcaaggaca
     4261 aggcagaagc ggcggctatc gcagcctgat attgttcaaa caggcagata gggcattttc
     4321 gtttacgcct ttgccaagaa cgacagggaa aacatttcgg ataaagaact ggacgtttac
     4381 cgaaaagccg ccgcatatta tctgaaatac acgcgggcag agctggcggc tttgaaagaa
     4441 gacggcatta tcacggagat agaatcatga aatacaaaag cgaggcatta gccgccattc
     4501 atgaaatgat ggaaggggct tacaacatcg gcgcaatcga caaaaagact atgcgcggct
     4561 ttgacaagtc ctgcctgacc gaaatcaaac cgttgagcgg cggagacatc aaggcaatca
     4621 gggagaagga ggcactatcg caagccgctt tcgccatcta tctcaacgtg ggaaaaaatc
     4681 acgtttcggc ttgggagcgg ggcgttaaaa agccgagcgg cgcggcgttg aagctgctga
     4741 ccatcgtcaa aaacaagggt atcgaagcca ttgcgtagcc gacttggcaa acggcaaaat
     4801 cagcaagttc acaatagacg cgctgctgaa tatgcctgcc aagacaggca agaccgccga
     4861 accgaatatc agggcatagc cgcataaatg cccgaccgca tcaaaccaag ccgaaacggc
     4921 ggcggtgcag acgacatagc ccgacagcaa ggcacggcgc agacgggcgc gaaacccgaa
     4981 acatccccgg tcgcaaggca cggggatttt tttgcgcccg ttgcaggggg gattggattt
     5041 aagcggcgcg ggcttgaagg caaaacgggc gggcgcagaa ctgtttaaat gccgtctgaa
     5101 tctcaaacga tttcagacgg cattttgaaa caacggctca aattctcgat ccccttccct
     5161 taacgccgac gttttttata gtggattaac aaaaaccagt acagcgttgc ctcgccttgc
     5221 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttaaatt taatccacta
     5281 tattaacgcg ccccttattt ctgacacttt gctcataaac cggcataacg gtcggcaaca
     5341 accgttttag attttctata cgggcattgt ttacagcatc agcataagac accgtactct
     5401 ggaataccgc ctaaaccctg cggatagttt tggatgtggt atcgacattg tcggcagact
     5461 tgtctaactc gaccgttttc aaataatctt tgacagatgc tgcattcagt gtcgcggaat
     5521 ccgcgtattt tgaacaaaag gtcgggctac tttatcacaa tagcagaaag atggaaaacg
     5581 gcggggattg gtgaaaggtc gtctgaaatg atattcagac gaccttgaac ggctgtgttt
     5641 taaatatggt atttattcat ttgtttcacc atcaggcagt ccaaaccacc ttaagggaat
     5701 cgtgttaaat tggtaatggc agttaacact gataatataa tctaaaacat ctttatattc
     5761 cggctttgta aaccaatcat tcaaaacata gacatattca acccgccaac ctaaagagtg
     5821 aaccaatttt gtatattgtt ttctcttaaa atcgcaagtt tgtaatttct catctactga
     5881 accgggagtt tgttggaatt tgatctcaat aataaataag gtatcacgga tgatgataaa
     5941 taagccattg tcgggtagta atcttttaga cagatggtct ttccaattaa tactgtatcc
     6001 cggtcttttc aaaaatcggt aaaactcata ttttttaaaa caataagcct tcaattctcc
     6061 attgaaccaa acttcataac ccgcattatc atatgttttt cttaagtcgt acccgggaat
     6121 ttcttcaaac aactttcgta agtctgtacg ttcttcgaat ctgagtcctg tttgtgtcct
     6181 tgcaccaccg attccgccaa taatcatttt tctaaccttt tttttgctaa ttcaaaaaaa
     6241 tcttcttcca gttcgcaacc acaaaatttc cgaccatgtt ttaaggcggc aacgcctgtt
     6301 gtgccgctgc ccatgaaagg gtcaaaaatt aaatctccga tgtttgaagc tgagagtatg
     6361 cagcgttcaa gcaaggggag tggtttttgt gtcggatgtt tgccgaatgt tttttcggct
     6421 ttgtttggag gaggaaatgt ccaaacacat ttcatctgtt tgccatcatt ttgtgctttc
     6481 atcatttcat aatgaaacgt gtgtttggct tttttatttt tctttgccca taagattgtt
     6541 tctgtcgaat gggtaaaaaa acggcaggac aaattagggg gaggattggg tttttcccaa
     6601 gtaatattgt tcaaaatatg gtatccgata gtttgcatca ggtagccgat taaataaata
     6661 ttatggaatg tgccgcaaac ccaaattgtg ccgtttggtt ttaataaggc gtaacacagt
     6721 cggagccatt cttcgtagaa ttccaaatct gccgccattc ctttggattt atcccagttg
     6781 cccttattga cggaaaccat ttgtccgttt tgacaactga agccgtcatt ggaaagaaag
     6841 taaggcgggt ctgcgaaaat catatcaaaa cagccgtttg gatatttttc caatatcttc
     6901 cgcatgacgt taagggaatt ttcgttgtag aggacaaggt cgtctgaaac gatttttttc
     6961 aaacgacttt gataaatggc gtcttcttca gccggtttgt caaaatcaat cataaattgc
     7021 caataattct ccgacttttt tacggctgct gccttttgcg gcaatattgc gttgtacttc
     7081 aattgttttc agaatagcaa aagaataaat atcccttgtg aattcagtat catgatttga
     7141 aatcaaaacg ccttgggaat cgggcgcaat ttgttgggct gactgggcaa gacggatttg
     7201 attgtctaaa ttgaaacctt ctttggcata agtggtaaat gaagcggttg ggcttaaagg
     7261 cgcgtaaggg ggatcgcaat aaaccgtatc ggtattgtct gtctgagata gaatcatttg
     7321 aaaatctccg cacattaatt caactctgtc agatttctga atgaaacctt ccatttcttg
     7381 ttggggaaag tacggcgatt tgtagcgtcc gaacggcaca ttgaataccc ctttgctgtt
     7441 gtaacggcat aaaccgttaa aagcatgacg gttcaaataa ataaatagtg ccgagcgttc
     7501 gtgcagattt ttgctgaagt tgaattgttc ccgcaattca tagaatcttg aatcgctatt
     7561 gttttcggaa ataaagaaag agcgggcgta gtcgatgaat ccggattttt cttcttttag
     7621 gatacggaat aagccgatta agtcagcgtt aatatcattg agcagatatt gttcgaaatc
     7681 caatgcaaga gacaaagcgg cagaaccgca aaagggttca atcaggcgtt ttcgtgtggg
     7741 agtaggtaga ttgtgttcga taaaggggac aagtttatgt ttgccgcctg cccattttag
     7801 aaaaggtttt gaagccatta ggatgccgag tacaagcgtg gttgattggc ttggaattgt
     7861 gccaaaacat cgtgaggaat acaatggatt tatatttggg ggcgtgtgta gagtgagtct
     7921 aaatccaata gaacctttcc attccaatat gttattggac ttatcaactc catctgcctt
     7981 aaaaaggccg tctgaaaccc tgtttccaag tttcagacgg cctttatccg tgtggctaaa
     8041 ccttaaaagc ggttagacga cgatgttcac cagtctgccc ggcacgacga tgattttctt
     8101 ggcgggcttg ccttccatga atttcactgc gccctcggtg gcgagtgcgg cggcttcgag
     8161 gtcggctttg gatgcgtcgg cggcgacggt gatttttccg cgcagtttgc cgttgacttg
     8221 aaccatgact tcgatttcgg atttgaccaa ggaggcttcg tcgactgtcg gccagcctgc
     8281 ttcccacaat ttcgcgccgt tcaattcgct ccacagggtt tcgcagatgt gcggcacgat
     8341 gggccacagc agacgtacgg cggcttccaa tacttcttgg gcgacggcgc ggccttgttc
     8401 gctgccggtg tcggttttgt cgtattggtt gagcaattcc atcacggcgg cgatggcggt
     8461 gttgaactgc tggcggcggc cgtagtcgtc gctgactttg gcgatagtgg aatgcagttt
     8521 gtggcgcagg tctttgagtt ctttagacaa accatcttgg ctgcctgcga acgctttgac
     8581 cgcgccgcct tgtttcaggt attcgtaaac ggtgcgccac aggcggcgca ggaagcggtg
     8641 tgcgccttcg acgccgctgt cgctccattc gagggactgt tcgggcggtg cggcaaacat
     8701 catgaacagg cgggcggtgt ccgcgccgta ggcgttaatc agttcttgcg gatcgacgcc
     8761 gttgtttttg gacttggaca ttttttccgt gccgctgatg acgacgggca ggccgtcggc
     8821 tttgaggacg gcggaaacgg ggcggccttt gtcgtcgaac gtcagctcga catcggcggg
     8881 gttgatccag tctttgccgc ctttgtcgtt ttcgcggtag taggtttcgc agacgaccat
     8941 gccttgcgtg agcaggcgtt tgaacggttc gtcaacattg accaaacctt cgtcgcgcat
     9001 cagtttggtg aagaaacgcg cgtacaagag atgcaaaatc gcgtgttcga taccgccgat
     9061 gtattggtcg accgcgcccc agtatttcgc ggcttctgcc gataccatgc cgtctgaaaa
     9121 cttgggcgac atatagcgga agaaatacca gcttgattcc atgaaggtgt ccatggtgtc
     9181 ggtttcgcgt ttcgccgcgc cgccgcagca cgggcaggca gtttcgtaaa actcgggcat
     9241 ttttgccagc ggcgaaccca tgccgtcggg tacgacgttt tcgggcagaa cgacgggcag
     9301 ttggtcggct gggacgggta cgtcgccgca tttttcacaa tggacgatgg gaattggaca
     9361 gccccagtag cgttggcgcg aaatgcccca gtcgcgcagg cggtattggg ttttcggttc
     9421 gcccgcgcct tggctttgca gcttggcggc aacggcatcg aatgccgtct gaaaatccaa
     9481 gccgtccaag tcgccgctgt tgaccaatac gccgttttct ttgtcggcat accattcttg
     9541 ccatcggttt gcgtcgaatg cgttgtcgcc gacggcaatg acttgttttt taggcagatt
     9601 gtatttggcg gcgaactcaa aatcgcgttc gtcgtgcgcc ggaaccgcca tcactgcgcc
     9661 gtcgccgtag ccccacaata catagttggc aatccacact tccagcttgt cgccgttgag
     9721 cgggttgacg acgtagcgac cggtcggcac gccttttttc tccatcgtcg ccatatcggc
     9781 ttcggcaacc gaaccggctt tgcattcggc aataaatgcc tgcaattcgg gtttgtcggc
     9841 ggctgcggcg gctgccagcg gatgctcggc ggcaacggca acataagtcg cacccatcag
     9901 cgtgtcgggg cgggtggtat aaacttgcag gaatttcgcg taatcgcctt ccaagccttg
     9961 tttgctgtcg tctgaaacag caaagcgcac ggtcatgccg cgagatttgc cgatccagtt
    10021 gcgctgcatg gttttgactt gttcaggcca gtgttccagc ttgtccaagt cgttgagcag
    10081 ctcttcggcg taatcggtga ttttgaagta atacatcggg atttcgcgtt tttcgatcaa
    10141 cgcgcccgaa cgccaaccgc gtccgtcgat aacttgctcg ttggcaagga cggtttggtc
    10201 gaccgggtcc cagtttaccg tgccgttttt gcgataaacg atgccttttt caaacagctt
    10261 ggtaaacagc cattgttccc agcggtagta ttcgggtttg caggttgcgg tttcgcgcgc
    10321 ccagtcaatc gcaaaaccca ggcttttgag ctgggttttc atgtattcga tgttatcgta
    10381 cgtccaagcg gcaggggcga cgttgttttt catcgccgcg ttttccgccg gcatgccgaa
    10441 cgcgtcccaa cccataggct gcatgacgtt gaagccgttt aaaagtttga agcggctcaa
    10501 tacgtcgccg atggtgtagt tgcgcacatg ccccatatgc agcttgccgc tggggtaggg
    10561 gaacatggag aggcagtagt atttgggttt ggaagcgtct tcggagacgt tgaaaatacg
    10621 ggcgtcgtcc cattttttct gcgccgcagg ctcgatggcg gcgggttgat attgttcttg
    10681 catagtcatt ctgtttttcg cttgaaaacg ttaggaattt aaaggaggaa ttataacagg
    10741 ttgccgatgt cggcgaatcg gcaaattgcc ggcaggatgc gtaaattcgc acgcgcatca
    10801 ttccgtatgc cgtacaaata caccgcgttt actgatacgc acgtttttta tgctaatatt
    10861 acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc tcttccgaac aaaaggcaga
    10921 cgggcatcgg gtaaaaaagg attctgcata tgaaaatcaa acaaatcgtc aaaccgggct
    10981 tggcagtatt ggcggcgggc attctgtctg cctgcgcaac caaaagcaac gtcaaagccg
    11041 acggagcgac cgacaatccg gttttcccga aaccctattc cgtaacgctc gacaacaagc
    11101 gcggcacatt cccgacctat gacgaactgg atcagatgcg ccccggcctg accaaagacg
    11161 acatctacaa aatcctgggt cgcccgcatt acgacgaagg catgtacggc gtacacgaat
    11221 gggattacct gttccacttc cataccccgg gcgtaggtat cgaccctgaa aacacgtccg
    11281 gcgtagaaga tgttactacc tgccaataca aagtgatttt cgataaagac aaatttgccc
    11341 gcagcttcta ctggaacccc gtcttcccga aagatgccgc ctgtccgccg cccgcaccca
    11401 aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc aaaaccgaaa cgtatccgcc
    11461 aataatccga catgccgttc cgcctgtttt tagggatatt gtgcgaccta tcgatggttg
    11521 cccccgtata tgcacagggg caaccggatg cggtcaacgc ttatattcaa aagaaaaaag
    11581 tcatcgtaaa cacatccaaa gcggaactct gttttgccga tgacaggcag tgccaccccg
    11641 tcctcatcgg tgttgccacg cccaagggga cgttcgggct gacgctgaac agtaccgaca
    11701 agcctggata cggcggcgaa gtcatcggtt tcaagcagga gggtgatttt cttttcgccc
    11761 tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa cgaacgcatc gcctccccgt
    11821 ccgtgtccga caggattatg accaacggct gcatcaacgt cagcgatgcg gtgtacgaaa
    11881 aactgcgtca ttattttgtg ttggaagtga tttgaaacag acggataccg cacgcgccgg
    11941 tatctgtttt cacattgccc cgatgcctga aacagactgt ccgccacgtc atgccgtctg
    12001 aaaccggcgc agatgccgcc aagccttcag acggcattgc ctgcccgctc cgaccgaaca
    12061 acaaccatct ttgggagaac cttatgcccg aacaaaaccg catcctctgc cgcgaactaa
    12121 gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga agacaaaaac gtcccccttt
    12181 tggaacgcct gcgcttcctg tgcatcgtgt cgtccaatct tgacgagttt ttcgaagtcc
    12241 gcatggcgtg gctcaaacgt gaaaacaagc tgcacccccg gcgcaggctg gacaacggca
    12301 aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc gcgctccctg atacggcacc
    12361 agtacgacct gttcaacaac gtccttcagc ccgagttggc gcgggaaggc atccattttt
    12421 accgccgccg caactggacg ggcgcgcaga aaaagtggat cgaaagctat ttcgaccgcg
    12481 aattgctgcc gattctgacc cccatcggac tcgacccttc ccaccccttc ccgcgcccgc
    12541 tgaacaaatc gctcaacttc gccgtcgaac tcgacggtac agatgcattc ggcagacctt
    12601 cgggtatggc gattgtccaa gcaccgcgca tcctgccgcg cgttgttccc ctgccgtccg
    12661 aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc catcctccac gcccacgtcg
    12721 gcaaactctt cccgggcatg aacgtcaaag gctgccacca gttccgcctg acgcgcgaca
    12781 gcgacttgac cgttgacgaa gaagacctgc aaaacctccg cgccgccatc caaaacgaac
    12841 tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt cgccgacacc tgtcccgcct
    12901 acatccgcga ctttctgctc gcgcaattca aactgaccgc cgccgaactc tatcaggtca
    12961 aaggcccggt caacctcgtg cgcctcaacg ccgtccccga cctagtcaac cgccccgatt
    13021 tgaaatttcc cacacacacg ccgggcagac tgaaagcctt gggcaaaacc gcgtccatat
    13081 tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc ctaccaatcg ttcgaccccg
    13141 ttgtcgaaat gatgcgcgaa gctgccgccg accccgacgt gcttgccgtc aaaatgacga
    13201 tttaccgcac cggcacgcgt tccgaactcg tccgcgccct gatgaaggcg gcactcgccg
    13261 gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt tgacgaagcc aacaacgtca
    13321 actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt gtacggcgtg ttcggctaca
    13381 aagtccacgc caagatggca ctggtcatcc gccgcgaaga cggcgtactc aagcgttacg
    13441 cccacctcgg cacgggtaac taccaccaag gcacatcgcg catctacacc gacttcggca
    13501 tcattaccgc cgacgaacaa atcaccgccg atgtgaacac attgtttatg gaaatcacag
    13561 gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag cccgtttacc ctgcacaaaa
    13621 tggttatcga ccgcatcgca cgcgaaaccg aacacgcaaa agccggcaaa ccggcgcgga
    13681 ttaccgccaa gatgaattcg ctcatcgaac cgaccgtcat cgaagccctg tatcgggcaa
    13741 gcgcggcagg cgtacaaatc gatttgattg tgcgcggtat gtgcaccttg cgcccgggtg
    13801 taaaaggctt gtccgaaaac atccgcgtcc gctccatcat cggcaggcag ctcgaacacg
    13861 cgcgcgtgta ttgcttccac aacaacggcg cggacgatac ctttatctcc agcgcggatt
    13921 ggatggggcg caacttcttc cgccgcatcg aaaccgccgc gccgattacc gcgcccgaac
    13981 tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcatt ggcagacaat acgcacgcgt
    14041 ggctgatgca gcccgacggc ggctatatcc gcgccgcgcc cgccgagggc gaatccgaag
    14101 ccgacctgca aaacgatttg tggacactgc tcggaggctg acccgcaccg ccccaatcaa
    14161 aaaccatgcc gtctgaaacc tttccgtttc agacggcatg gttttacagc aatctaaaca
    14221 gggcggaccg gagtcaaaaa cacaccttcg ccatttccgc ataagcactt cccctatacg
    14281 ctcccaaccc caatccgccg cattccagac ggcattgcct ttccgacagc cgaacaaaac
    14341 cccgaatccg tctgcacggt tcggggcata tctccaatac gggcatcgtg ctccggaaaa
    14401 ccgtcaaatc cgcatcggca tcacaatata tttgaaattc ggattgttcg gcacggtaaa
    14461 cagcgtcgag cggttggcat cgccgaaggc aagctgcata tcgtcggaat ggatgttgcg
    14521 caacacgtcc atcagatagc cgatattgaa accgacttcg agttcgccgc cctgataggc
    14581 aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg ctgcacacga cgctcaacag
    14641 gccggattgc aggaacaggc gcgcgccacg gaatttttcg ttggcaagaa tggacacacg
    14701 ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa atcttgtcgt tgtccaaagg
    14761 aatcacgcgg ttgaaatcgg ggaatttgcc gtcgataacc ttgctgacga tggtcgtgcc
    14821 gttgcattgg aaacgcacct gtttgtccag cagctcgatt tgaatcggat cgtcggggtt
    14881 gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc aaaatcactt cggcgcgcgg
    14941 caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg tgtccgtcgg tcgccacaag
    15001 gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg agataatagc ggatgtcctg
    15061 caccgccatg ctgtactgca cttgcgacag catggttttg aaacgctcct gctccagcga
    15121 gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga aaatcggcgg caggcagggt
    15181 ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga cggctgtcgt cccaatccag
    15241 cgacaccagc gcaccggcag gcagcgcgcg caaaatgtcc tgaaatttct tggcattggt
    15301 ggtgatgcgg aagtcgcccg cgccgccctc gggacccgca gtgtcgattt ggatttccaa
    15361 atcggttgcc aagagtttgg tctgaccgcc ttttccctca atcaggacgt tggacaggat
    15421 gggcagggtg tggcggcgtt cgacgatgcc ggtaacggct tgcagcggct tgagcaggct
    15481 gtcgcgttcg gcttgtaaaa tcaacatttt cgctccttaa atcggttgga ttttttaccc
    15541 ttcggacggc atgggcaata tcatgccgtc tgaaaacgtt ttccatcagt tttgaatcag
    15601 aatcagcagc ttttcataat cctgagccaa ttccggatct tcttcgcgca gtttcgccac
    15661 tgccctgatg ccgtgcataa cggtcgtatg gtcgcgtccg ccgaacgaat cgccgataga
    15721 cggcaggctc aaagtggtca gttctttggt caggctcatc gccacctggc gcggacgggc
    15781 aatgtttcgt gtccgtttct taccgagcac atcgctgatt ttgatgcggt aatatttcgc
    15841 aacggcatca ataataatat cggcggtgat gactttgtgc ttctcggcaa taatgtcctg
    15901 caaagcggta cgcgccaaat cgatgtcgat gacgggacgg ttcataaagc ggctgctcgc
    15961 tccgacgcgg ttgaacgcgc cttcaagctc acgcacgttg gaacggatca gattggcaat
    16021 gaacagcgcg gcttcgtctt caatactgat gcccgccgct tccgcctttt tctgcaaaat
    16081 ggcgatacgc atttccaatt cgggcggctc gagttccaaa gtcagtcccc atgaaaaacg
    16141 ggatttgagg cggtcgtcca taccttcgat tttggcgggt aaaacatcgc aagtgaggat
    16201 gagctgtttt ttctcgttat ggaaatgatt gtacagatag aagaattcct ccatcgtacg
    16261 gtctttgcct ttgatgaact ggatgtcgtc gataatcagc aggtcgtatt gcttgtattg
    16321 ctgcttgaac acgtcgtagg tattgttgcg aaccgccttc ataaagctgc ggatatagtc
    16381 gtccgaatgc atatagcgca ctttggcatc gggacggttt ttcaacagtt cgttgccgac
    16441 cgcctgcaca aggtgggttt tacccaaacc cgtactgcca tagaggaaga acgggttata
    16501 actctgtccc ggactttccg caatcgcctg cgcggcagct gccgcaaggc ggttgccctt
    16561 gccttctacc aacgtatcaa atgtgtaatc cggagacagg ttggtttgtt cgtaacgcgc
    16621 ctcttccgca tcacgctgcg catccgtccg cgctttcgca agtacctccg attccggacg
    16681 ggaagcagac ccggcagtat ggcgcggctc atgcgggagg tttttcatac gttccgccaa
    16741 aatatccgcc gccgttttcg cctgtacgga tttggcagac ccttcagacg gcagctcgtc
    16801 caacagaacc tcctgcacgg gcatttcctc cgacaccgca tgcaaggacg gctcagcagg
    16861 ttcgacagca ctttcaaccg ccgccatctc ataacgcacg ccttctcccg gtttgaatac
    16921 gaaggcggaa cggccagcag ccaactcttc cctcaccgct tctattttcc cggcaaactg
    16981 gctcttaagc atattgcagg caaactggtt cttgccgtac accacccata cgccaccctc
    17041 ctcaccgacg gtaaggggcg caatccattg cgcaaactgc ccttgaggca agatatcgtg
    17101 aagacggcgg aggcacagcg gccaaaactc tgctaatgtc atggataggc tcgaatcggt
    17161 aaaaatgaaa tcgaaaacaa agaaaatata atattttcaa aaagaaaaca aatctgttga
    17221 acgcgcatcg gttcaaaacg cgactgcccg attataccga ctcacgaata ttttatccac
    17281 aacccgtgca aaaatttatc cacagaaagg cggcagaaat ccgcaggcaa tcgggcaatc
    17341 ctcctgcaaa gtttctatat tgattgacaa aagcggcaaa ttggagtgta attcacggtt
    17401 taattatcta cccattctat tttaggaaac atcatgaaac gcacttatca accttccgtt
    17461 accaaacgca aacgcaccca cggcttcctg gtgcgctcca aaacacgcgg cggccgcgca
    17521 gtattggccg cacgccgcgc caaaggccgc aaacgcctag cggtataatt ttggactacc
    17581 gcttcggaag gcagtaccgc ttgttaaaaa cggatgattt ttcatccgtt tttgcattca
    17641 gaaaccgccg cagccgcgac ctgctgcaag tttcgcgttc aaacggcaac gggctggacc
    17701 atccccgcat cggtctggtg gtcggcaaaa aaaccgccaa acgcgccaac gaacgaaatt
    17761 atatgaagcg cgttatccgc aactggttta gattgaacaa aaaccggctg ccgccgcaag
    17821 atttcgtcgt gcgcgtccac cgtaaattcg acaggtctac cgcaaaacag gcaagggcgg
    17881 aactggcaca actcatgttc ggcaaccccg caaccggatg cgggaaacag gtatgattag
    17941 aacggtactc tgcaggcaag gttcagacgg caacgggttt cccatacaag gaacatcccg
    18001 atgaactttc tattgtccaa actcctgctg ggactgatac ggttctacca atattgcatc
    18061 agcccgctga ttccgccgcg ctgccgttat acgccgacct gttcgcaata cgcggtcgaa
    18121 gcggtcaaaa aatacggcgc attcaaaggc ggccggctcg ccatcaagcg cattgcacgc
    18181 tgccaccctt tcggcggaca cggacacgac cccgttccct gacccgacgc aatattcaaa
    18241 ttgcacgctt tccttttatt tcccatcggt ttctatataa tgccgtctga aacttcgggc
    18301 tggcggcacg accgtcgggt atgaagcccg cccttattcc ccgtctatcg gaacacgcaa
    18361 cctgcggcat ttccgaccat tcaggaaact ctatatggat tttaaaagac tcacggcgtt
    18421 tttcgccatc gcgctggtga ttatgatcgg ctgggaaaag atgttcccca ctccgaagcc
    18481 cgtccccgcg ccccaacagg cagcacaaca acaggccgta accgcttccg ccgaagccgc
    18541 gctcgcgccc gcaacgccga ttaccgtaac gaccgacacg gttcaagccg tcattgatga
    18601 aaaaagcggc gacctgcgcc ggctgaccct gctcaaatac aaagcaaccg gcgacgaaaa
    18661 taaaccgttc atcctgtttg gcgacggcaa agaatacacc tacgtcgccc aatccgaact
    18721 tttggacgcg cagggcaaca acattctaaa aggcatcggc tttagcgcac cgaaaaaaca
    18781 gtacagcttg gaaggcgaca aagttgaagt ccgcctgagc gcgcctgaaa cacgcggtct
    18841 gaaaatcgac aaagtttata ctttcaccaa aggcagctat ctggtcaacg tccgcttcga
    18901 catcgccaac ggcagcggtc aaaccgccaa cctgagcgcg gactaccgca tcgtccgcga
    18961 ccacagcgaa cccgagggtc aaggctactt tacccactct tacgtcggcc ctgttgttta
    19021 tacccctgaa ggcaacttcc aaaaagtcag cttctccgac ttggacgacg atgccaaatc
    19081 cggcaaatcc gaggccgaat acatccgcaa aaccccgacc ggctggctcg gcatgattga
    19141 acaccacttc atgtccacct ggatcctcca acccaaaggc ggacaaagcg tttgcgccgc
    19201 tggcgactgc cgtatcgaca tcaaacgccg caacgacaag ctgtacagca ccagcgtcag
    19261 cgtgccttta gccgccatcc aaaacggcgc gaaagccgaa gcctccatca acctctacgc
    19321 cggcccacag accacatccg ttatcgcaaa catcgccgac aacctgcaac tggccaaaga
    19381 ctacggcaaa gtacactggt tcgcttcccc gctcttctgg ctcctgaacc aactgcacaa
    19441 catcatcggc aactggggct gggcgattat cgttttaacc atcatcgtca aagccgtact
    19501 gtatccattg accaacgcgt cctaccgttc gatggcgaaa atgcgtgccg ccgcgcccaa
    19561 actgcaggca atcaaagaga aatacggcga tgaccgcatg gcgcaacaac aggcgatgat
    19621 gcagctttac acagacgaga aaatcaaccc gctgggcggc tgcctgccta tgctgttgca
    19681 aatccccgtc ttcatcggat tgtattgggc attgttcgcc tccgtagaat tgcgccaggc
    19741 accttggctg ggttggatta ccgacctcag ccgcgccgac ccctactaca tcctgcccat
    19801 cattatggcg gcaacgatgg tcgcccaaac ttatctgaac ccgccgccga ccgacccgat
    19861 gcaggcgaaa atgatgaaaa tcatgccgtt ggttttctcc gtcatgttct tcttcttccc
    19921 tgccggtctg gtattgtact gggtagtcaa caacctcctg accatcgccc agcaatggca
    19981 catcaaccgc agcatcgaaa aacaacgcac ccaaggcgaa gtcgtttcct aaatgccgca
    20041 gcatgaaaaa tgccgtctga aacctgttca gacggcattt ttattgttca ccccctatcg
    20101 gggcggaaat cttcaacctg catacatcac aaaaatcgtc gggcgttttt tcagattggg
    20161 catttccttc atcctgcgcc aatccgcaac cgttttactg accaccgact gagtcggcaa
    20221 cgtcaaatcc gtagccgtgc acaagcgcgt ttcaggatgc aggttttcca ccgcatccgc
    20281 caacaacaca tcgttgcgat acggcgtttc gataaacagc tgcgtttcat tctgctggcg
    20341 cgaacgttgt tccaaagcct tcaaactctg aatccgctca tttttttcag acggcaaata
    20401 acccttaaac gcaaagttct gcccattcgc acccgaagcc atcaaagcca gcagcaggct
    20461 ggaaggcccg accagcggac gcacttcaaa accgtgttta tgcgccaaag ccaccagatt
    20521 cgcacccgga tcggcaacag ccgggcaacc cgcctcgctg acaatgccca tactgcgccc
    20581 ttcttgcaaa ggtttcagca attccagcaa agtcttcaaa tccgtgtgtt cgttcagcgt
    20641 ttgcaaattc agttcgcgga taggcattgt cacgcccaaa tgtttcaaat gcgcgcgcgc
    20701 cgttttttcc gcttccacga caaaatccgt cagcccgaca atcgcctgtt gttcatgcgg
    20761 caacaggcac ggcgtatcgg gcgtacccaa aggcgtagga atcaaataca aaacaggaga
    20821 catcattccc tcactcatcg gttaaaaatg ccgtctgaac ccttcagacg gcataaacgg
    20881 gcagttacaa aacatccacg ccctcatttt tcaaaaaatc gaccagacgg aaaaccggca
    20941 aaccgattaa agcattcgga tcggtactct caatcctttc aatcaacaat gcacccaaat
    21001 cctcactctt cagcgcacac gaacaataaa ccgcatcagg ctcgcgctcc aaatagcgga
    21061 ggatgtgcaa ctcgtccaac tgcctcatca cgaccaccgt cttatcgata tgccgctgca
    21121 tcctgcccgt aaccgtattt agcagaacga ccgcgctgta aaactcaatc tccctgccgc
    21181 tcaaatgcat caacatcttt tgcgcattag caaggttcat cggcttgccc cactgcctgc
    21241 cgtcgcacca cgccacctgg tccgcaccga caatcaacgc ctttgggaaa cgcccggtca
    21301 acgaccgcgc cttaccctcg gcaaggcgca atgccgtctg aggggcggat tcccccaaca
    21361 tcggcgtttc gtcaaaatcg ggggacgccg cctgaaaggc aatgccgagc ctttccatct
    21421 gttcgcggcg gaaaaccgaa ctcgtaccca aaatcaaagg cagttccaaa cccatcccat
    21481 cctccttacc gttgaaaaca cgcccgaagg ggcggtaaaa tccagccatg cgccgaaaca
    21541 cggatacccg ccttcggcgt accgcaacat ttttcttaaa aatattgacg ttagaacatc
    21601 taaattatat cataccccgt ttatgtcaga ccctaatttg attgacccgg aaattttcgc
    21661 cgccgaaggg cagaatctgc aaggcagttt tctgctggaa gaattggatg aacgcgtcag
    21721 ttcgcacgat tatcccgccg acaggcagac caaaatatcg tttacactga ccggcggtcg
    21781 cgaccggctg caacgcctgt tcctcgacct gaacgtcaaa gccgatatgc ccctgatttg
    21841 ccagagatgt atcaaaccca tgccgttcat gcttgatgaa agcagccgta tcgtcctgtt
    21901 ttccaacgaa gagtccttgg acgaatccat gcttgccgac gaagaactcg aaggcatact
    21961 gattgaaaaa gaactcgacg tgcgcacatt ggtagaagac caaatcctga tgtccctgcc
    22021 cttttcgccg cgacacgaag actgcggcga caatgggaca ctggaagaag tcaatcggga
    22081 caaacccaac ccctttgctg ttttggcagg tttgaaaagc aattgattag gacacagttt
    22141 atttatctag gagcttgaaa tggccgttca acaaaacaaa aaatcccctt ccaaacgcgg
    22201 tatgcaccgt tcgcacgacg cgctgaccgc gcccgcactg tctgtcgaca gcacaaccgg
    22261 cgaagtacac cgcccgcacc acatctcccc caacggtatg taccgcggcc gcaaagtggt
    22321 caaagccaaa ggcgaataat ccctattcga ctgactgaaa aagccagaac attgccatgc
    22381 aattactggc tttttttgca ttggacgcac catccgtcca aactttcgcc atacgtcaac
    22441 acacaggggc aaagcgttcc gtataataac ccgtgaaaat atagtggatt aacaaaaatc
    22501 aggacaagg
//
LOCUS       DAWHXS010000023        22437 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWHXS010000023 DAWHXS010000000
VERSION     DAWHXS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22437
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.023"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(381..2354)
                     /locus_tag="WIE22_000669"
     CDS             complement(381..2354)
                     /locus_tag="WIE22_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ4126801.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRHRDSLLEALQD"
     gene            complement(2365..2925)
                     /locus_tag="WIE22_000670"
     CDS             complement(2365..2925)
                     /locus_tag="WIE22_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ4126802.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2922..3533)
                     /locus_tag="WIE22_000671"
     CDS             complement(2922..3533)
                     /locus_tag="WIE22_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ4126803.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVPSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            complement(3533..3976)
                     /locus_tag="WIE22_000672"
     CDS             complement(3533..3976)
                     /locus_tag="WIE22_000672"
                     /inference="COORDINATES: protein motif:HMM:NF016736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ4126804.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLRRRNPTTASGTLTVSGIAQSMLSDG
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARRN"
     gene            complement(3990..4337)
                     /locus_tag="WIE22_000673"
     CDS             complement(3990..4337)
                     /locus_tag="WIE22_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ4126805.1"
                     /translation="MDKELNPDTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(4437..5105)
                     /locus_tag="WIE22_000674"
     CDS             complement(4437..5105)
                     /locus_tag="WIE22_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ4126806.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(5102..6247)
                     /locus_tag="WIE22_000675"
     CDS             complement(5102..6247)
                     /locus_tag="WIE22_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ4126807.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(6237..7568)
                     /locus_tag="WIE22_000676"
     CDS             complement(6237..7568)
                     /locus_tag="WIE22_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ4126808.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAVAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAAINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDMARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(7568..9739)
                     /locus_tag="WIE22_000677"
     CDS             complement(7568..9739)
                     /locus_tag="WIE22_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ4126809.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            9894..10476
                     /locus_tag="WIE22_000678"
                     /pseudo
     CDS             9894..10476
                     /locus_tag="WIE22_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10520..10789)
                     /locus_tag="WIE22_000679"
     CDS             complement(10520..10789)
                     /locus_tag="WIE22_000679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126810.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(10729..11094)
                     /locus_tag="WIE22_000680"
     CDS             complement(10729..11094)
                     /locus_tag="WIE22_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126811.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(11098..11475)
                     /locus_tag="WIE22_000681"
     CDS             complement(11098..11475)
                     /locus_tag="WIE22_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ4126812.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(11540..12949)
                     /locus_tag="WIE22_000682"
     CDS             complement(11540..12949)
                     /locus_tag="WIE22_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ4126813.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGVAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(12952..13149)
                     /locus_tag="WIE22_000683"
     CDS             complement(12952..13149)
                     /locus_tag="WIE22_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ4126814.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(13146..13796)
                     /locus_tag="WIE22_000684"
     CDS             complement(13146..13796)
                     /locus_tag="WIE22_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ4126815.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(13786..14208)
                     /locus_tag="WIE22_000685"
     CDS             complement(13786..14208)
                     /locus_tag="WIE22_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ4126816.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(14208..14606)
                     /locus_tag="WIE22_000686"
     CDS             complement(14208..14606)
                     /locus_tag="WIE22_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126817.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(14671..15579)
                     /locus_tag="WIE22_000687"
     CDS             complement(14671..15579)
                     /locus_tag="WIE22_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ4126818.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(15579..16640)
                     /locus_tag="WIE22_000688"
     CDS             complement(15579..16640)
                     /locus_tag="WIE22_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126819.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKSETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            complement(16871..17287)
                     /locus_tag="WIE22_000689"
     CDS             complement(16871..17287)
                     /locus_tag="WIE22_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ4126820.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNRIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(17415..18740)
                     /locus_tag="WIE22_000690"
     CDS             complement(17415..18740)
                     /locus_tag="WIE22_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ4126821.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(18727..20295)
                     /locus_tag="WIE22_000691"
     CDS             complement(18727..20295)
                     /locus_tag="WIE22_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ4126822.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(20295..21917)
                     /gene="terL"
                     /locus_tag="WIE22_000692"
     CDS             complement(20295..21917)
                     /gene="terL"
                     /locus_tag="WIE22_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ4126823.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDALPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIATANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            21929..>22437
                     /locus_tag="WIE22_000693"
     CDS             21929..>22437
                     /locus_tag="WIE22_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126824.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDG"
BASE COUNT         4527 a         6333 c         5941 g         5636 t
ORIGIN      
        1 tgatagattc ctgccgcgtt tgggggctgg attcccgctt tcgcgggaat gacgaaagag
       61 gcgggaatga cgaaagcggc gggaatgacg aagagtggca ggattggcgg ggcataggtt
      121 tcgggggatt ggcggcgcgg ccggtttcgg tcggattgct tggatcggcg ggctgaagcc
      181 catctctaca gcccatctct acagcccatc tctacagcca tctctacagc catctctaca
      241 gccatctcta cagccatctc tacagccatc tctacagccc atctctacag ccatctctac
      301 agccatctct acagccatct ctacaaccca tctctacaac ccgtctctac agccatctct
      361 acagccatct ctacagcccg ctagtcttgc agtgcctcga gcaagctgtc tcggtggcgg
      421 cggtttcggg ggccggttga tgttcttcca ctctctcttt gcggttcttc gttggtttca
      481 ggtatttcgg ggcctatcgg ggagctggcg gcgtttcccg aggcggcttt gccgatggcc
      541 atccagtcgc aactgccttc gtaccagttg gtaataatgg cggcggcgtg gttttttgcg
      601 cggataaaca gccagttgtg ttgccttcga tcgccggtaa cgcgtcccga atggcgttcg
      661 gatacgaagc atttgacgtt gccgtcggca aaggcgacgg ggaagacgac ttcgttttct
      721 atggggccgc cgcttcgtgt gaacctgtag ctgccggtct gtatcattgt gccgtcgggg
      781 aatctgacaa tatccacgcc gccgatggtt tggcgtgtga attgcgcggc aacggcggtg
      841 ctgatttttt cgtccagccc ttggatttgg gcggcggtgt gggtgtgggc gcggtctgct
      901 ttgtcttgga ggttttgcgc cagcgtgccc gcgtccagcg cgccggcgtt gacggtttcg
      961 ccgtgggact ttatccagaa cacggcttcg cccaaggtgt cggcggcttt gatgcacagt
     1021 tttaaaacca gggctttggg gcggttttcg tcgccgcctg tcgccatttt gctgtccggg
     1081 cgcggggtta aaaagccgtt gtcgtttaaa ttttcgtccg tccaagtcga tacgagcgcg
     1141 cttctctgcc tttcgttgtg gtcttcgtaa ccgagggctg ccgcgtgcgg gtggtttacc
     1201 cagtgtgaaa atactttgtg gacgtgccgt ttgatttcgt cttcctgctt cgttccgact
     1261 gccaagccgt tggcggcgtt gcggataaag cggtcttccg cctgcgggac gttttggatg
     1321 ctgccgtatt ttccggtcag cagacggtac agctcgggat aagcggtttc ggttacgcgc
     1381 gtgcggatgt cgtcaaacgc cagccagccg gtcgggattt ggtcggacgg aaaccatgcg
     1441 gtgatgccga tgtcggtacg gcttaaatcg ggcaggcggt tgctgttgcc cagggcgcgg
     1501 tagaggtcgg ggaaggtgtt ttgtgcaaag gtcgtgccgt tggctttgag atagcctgcg
     1561 gggtttcgga cggctttggg aaaggatacg atcgcgccga cggggatgcc gtccgaattg
     1621 agttttttcc atgcggacca gttgtcgttt gcgctgctcg tttggtggcg ttcgtacacg
     1681 tcggacgtgt aggcgggata acccaactgc ctgcaccagc cgtgtttcgt gccggcgatg
     1741 acttggatat ggcaggcggt gttttcaacg ggcaggttgg agctgccgcc cgccgtcggc
     1801 agggaataga tgccgtctgt tttgagggtg ttcaaatcgc cccggaaggt ttcgactttg
     1861 aaattgccga tgccgtatcc ggacagtgtg ttcggcttac cttcgatgtc ttcggcaaaa
     1921 cgcggtttgt tggctttgtg gaagatgtcg tggttgttgt aggaaatgcg gttgtttgcg
     1981 ccgtgcaggc ggagggtgtc gtcgccgaag acgatgtggg cgttgtcggc atccgcgccg
     2041 atgtaggctt tgtggccgcc gttccagttc gtctggttgg cggataaatg gatgccgctt
     2101 tggaattggg tttgggcggt aaaggttttt tggccggtga tggtttggtc gccggtggta
     2161 actacagcca gcctatttag tgctgccagt aattgattag gctgcgactt atccggctgg
     2221 ataccggccg cagtcagcac ggccagcagc tcggattgca cttggttgag ccaccacgcg
     2281 ggcaggatgg tgcccaattc ggacacgccg tcgccgtcgt ggaaggtctt gtcgggggtt
     2341 tcgatggggt gcatggttta gtccttatcg gtaggtaaat cggatagcgg tgtgggcggg
     2401 cttgaggcgg ttgaacaggc tctcgatcac ggcatcgctg taatcggtca gcctgtcgcc
     2461 cgccgccgag ataccggcgc ggaatcgggt aatgcggttg ttgccgccgc gcacgttaac
     2521 gtgccacacc cacatgattt cctgcggcgc aagacggtcg ccggcgcggt ttacaccggc
     2581 gcggaacggc tgcggttcgt cgatttggat ttgatagccc gccgcctcgg ccaaacgcac
     2641 aaaataagga atactcaagc cgcctgtttc gtttagcttg gccatgacgg ccaacacacg
     2701 gcgctggcgg tttttgcccg taccgtccaa acctaatacg cgctcccaat cggccagcat
     2761 ttggccggcg ctgcgcgggt cgacggcatc ggcaacgctt tgagccgatt ccgccaccgc
     2821 atccagcgct gcgccgtcta tttctgcctg cgcccgcaca cgcggggcat tgcgggcata
     2881 cgacacgggg ggcaacaggc cccgcaagat gtcttgatag ctcatgacgg catctccgtt
     2941 acattaatcg cgcccgcttt aaaccactcg atgcggttaa ccgtatcggc ggcacggttg
     3001 gccgtcggcg cagtcagacg gcggtcgatc acaccatcca cattgctgat agccgcctcg
     3061 atttgcgaca cagtcaggcc gtcaccgggg atcagggtgt cgaaatattc ttttagggcc
     3121 gtccgtatgc ggcgcttggc ctcgtccaag tcgatgccgt cgagcttgac ttgcacggta
     3181 acaggcaccg ccgttacggt tggcttgagt accagcgcat tttttgccgt taccgggcgc
     3241 atctcgtcga tataagcctg tacgcggcgc acagtttctt ccgatggcac accgtcggcg
     3301 gaggtaatgg caatatccac cgtacccaag ccgcggcgca gcggataaac atatgcgctg
     3361 gttacgccgt caacactcaa cgcccagttt ttatagtcgt aacggttgcc gcctgcgggc
     3421 ggtcggcgga tgatttccaa cagacgcgcc agcagtgagg catcgctttc tcggtcggtg
     3481 ccgccttgta cggtaaggcg gcattcggtg gccacaccgg cgggggcggc catcagttgc
     3541 gcctcgccgt cgccacattg gcggccgcgc ccggctcgtc ggcgattgcc ggtatttccg
     3601 ccgtgccgcc gctgccgata acggcgcggg cggtagtgcg gtaaaaacgc tggccgatac
     3661 gcacttgcag gccgtctgaa agcatggatt gcgcaatacc gcttacggtc agcgtgccgc
     3721 tggccgtggt aggattgcgg cggcgcaagc cgcgcatgga ggcatgccgc tccaaatact
     3781 cgcggtcggc ggtatcaggg aaaatctgcc gcacaatcca gctttgatgc gcatattgcc
     3841 cttcggcgca gctggccaaa cggctggcgt gcacataatg gtcgctgtcg gggctgatat
     3901 cggcatccgg ccacaggctt ttggtatcgc gcaggatacg ctcgcggatt tgctcaaatg
     3961 tcggcgtttc aaacacgttt aaaacctctt taaatcacgg gcacttcgtg gcggtaatca
     4021 aacccgcccg ccgccgtatc cacccggata tgcaggatca ggcggccgtc gtgcggctgc
     4081 tctgcgcgca cggtaatctt gtcggcacgc ccgctcttaa caatcggttg cagtgcctca
     4141 tcggcatatt gctcggccaa caggctgacc cgttgcaggt ctttttcgcg ctgcaacaaa
     4201 tgcagcagcg agccgagcgt tttatccgcc caccagctgc ccaacggtgt catcaaacgg
     4261 atatacacgg cattttgcag cgtatcgacg gtgcggccgg tatagtcgcc ggtgtcgggg
     4321 tttagctctt tgtccatgtt ggcattttgc cgttttcaga cggcctcacg gagtacacgg
     4381 gcttcaatcc cgcacgcaca aaaaagcagc ccgaaggctg ccaaaggtct gcttgtctat
     4441 gccggttccg ccggtaaggt tttgccgccg atgctgtcgg tatgcgggtg ctggcgcaac
     4501 gatatattgc cggccaccac gtcgccgtcg gtgttaaagc tgccgcccgt ttggttaaca
     4561 tcgccgctaa aggtggctcc gtcgccgccc tcgacggcca tgccgccgtt gccgttgatt
     4621 tggccttgcg ccgtcaacac tgcactggtc tccaccaacg gagcgttaaa tttggcatcc
     4681 gtggccgcat taacctcgta ttgtttgcag ttaacccggt acacgtcgca atcggcctca
     4741 atgattttgc cttgcttaat cacgattttt gcaccctcat gattaaaaat cgccgtctcg
     4801 ccgggcttaa ggtttttgat gcggtagctg ccgtgctggc tgcacacaat cacaccgtgc
     4861 gaagtattgc cgcccagcgg tatcactacc gcttcgctgc cgtcgggcgg atggctggca
     4921 aagccgtatt cctgcaaatg ttcaaggtct tgcagggttt cgtcggccaa gccgctcaac
     4981 tgcacgcgct gtatcggctc ggacgacacc accagcgtga tttttccccg aaaggccgcg
     5041 cgcagggttt cgccgatatt tttagcagtt tgtgccgttt ttttcgccaa tttactcaaa
     5101 ctcattcaaa caccgccgtt tctgcttgtt ttttgccgcc ttttttgccc ttatggctca
     5161 cgcctttgcg tttgcctttg cgcttgcgcg ccgcctcggc ctttttgggg taagcgtcgg
     5221 gtgtccaaat accgtcctct ttgagccgca gctcggtttg cgtgccatcc atgcgggata
     5281 gcataaaccg ccgccccatc agaaaaaaca ccgcatcgat accgtgctcg tcgtcgatca
     5341 catgcacacg ctggccaggt tgccacaata cgccgtcgcg ggttttatgg ccgcccacgg
     5401 ttatcgtgag tgtaaatccc tccagccgcc agtcggccag ctgcttttta gcctgctttt
     5461 gcaatgcggc caaattgtcg gcatcggaca ccaccaccgt tttagggcgg tgcagcgtca
     5521 tcgtcgggtc tttgtacacc cactttaaat cgtgtttggc gctgtcgccg ctgcggccgt
     5581 gcgattgcgc caaaaaagta acctcggaaa agcggttgtc ggtatcccac tcaatgtcca
     5641 tgcgctcgat attgcagcgg ctgtcggtgc ggctccaaca caatgtcgcc accggcgggc
     5701 tgctgtaatc cgcaccgccc accaccaacg tgccgtccgg ctccagccac ggatgcagcc
     5761 cgaccgagtt ggcaatatgg gttaatgcct gccatacggt ttcgcccggc tcgatgtcga
     5821 ttttgtccaa agcggggttg ttttcggcct taagcaccac cgctttaatc tgcggccacg
     5881 gcgcggccag ctttttggct gcatccaata ccgtcatgcc ctttacattg agctgcggcg
     5941 cggagcaatc caccaaaaaa ccggccaaat cacgcccgct caagctcaac tcgcggctgc
     6001 ccttgctttt gccgtggcgc tggctgccga tgatgcccgt catcacgatt tgcccgtcta
     6061 tcactacctc gcagctctct ccgcttaaat cgggtatggc cgcctccggc cccaacctgc
     6121 cgatgacaaa atcgaagctg tcggcaggga ttaaaaagtc gctgtcgatg tcgtagcgct
     6181 cccagtggcg gtgctcttta ccgcccacgc gcaccgacac ggcatagccg tatgaattat
     6241 tttgcatagc tgttgaccaa agtgccgcgc ttgataaacg cggggtggtg gatatgggga
     6301 ttgagccgca ccagctctgc tgcgcgggcc atatcgccgt aaaactcgtg ggcgatttgg
     6361 tgtatcgtac cgtcgattgg ggcttggcgc acaatcagcg gcggcttttg gttgatggcc
     6421 gccgcaacca acgcattcag acggcctgcc gccgcgcgca gggattctgc cgtttggtaa
     6481 gcctcggtat acacggcgtt ggccgtcagc ccaccagact cggcagcagc cgtctgcacc
     6541 gcccgcaagg cggcaatctc ggcctgcata cggcggcgca tggcgcggtt aacctcaatc
     6601 aaatcgggcg cggtcatctc ttcgccatgc gcctcgatta atgccgttgc caccgacaac
     6661 agcgatgacg tggataacag gcgcaccgcc tgcgctaccg gctgcacctg tttggcggtt
     6721 aaccggttca ggcggttttg caggccgtct gaaaagcggc cggtcagtaa attatcaggg
     6781 atagcggcgg cacggtcggc aacagccaca gccccgtcaa accgctgtcg cggcgaccaa
     6841 ccggcatggt gcatggcatt atcggccaaa cccgcctcac ggcgtatgcc agtatctacc
     6901 atgaccgata tatccgcaaa cagcttggcc gagccgtttt taaacgccgc tgcgctgtat
     6961 ccgccccgat cgggaaaggc aattttgtcc aaatcaaaca aacggcgcac cgcaccaaac
     7021 gtacccgatg ccgcactcca aatgcccagc gcgctgcccc ataaagccga tacgcccgca
     7081 tccaccgcca acaccgcatc aacaaagccg atagccgcct cgcggtaggt gtcgatatca
     7141 gcaatcaacg cctcaagctc gaccaaaaag gcgttttcaa aaacaaagat ttcctgcgct
     7201 tcggccgcct cgcggaaagt aatatcgatg cccgcataat ccacataatc ggcctcatgt
     7261 cggtaactcc atgatgccgc aatcatgttg tgcatccgcc cccaaacagg gtgcaccagc
     7321 acgccgccgc ccggctgctc cagcgcatcc agcagctttt tcagacggcc tgcatagccc
     7381 ttgccccaaa acaccgcatt aatctgcacc tgccgcccgg tcatgcccat gtcttcaagg
     7441 tcgataccct gcacaaacgg ccgcgcatgc tcggccaatg ccttgccgtt gctctcgtcc
     7501 accacctcaa tatcaaagcc gacgcccttg taagatgcgt cctgcaataa ggtatgccat
     7561 ccgctcatta ttgacccgct ccacggccga acatggccac ttgataccgc gatacctcat
     7621 tcgcgataac acgtccgtcg atattcaggc tcacattggt attgatggtc tgacttgccg
     7681 acgccatcgc actttgcact tgtgccaaac ctgttgtaac tgcagccgta tcctgcgcca
     7741 atgctgcctg atacgccgcc gtctgctgct gaatggctgc ctgatacgcc gccgtctgag
     7801 ttgaaaacaa cggagacaac atcttatcgg taggctgcgc tgacggagca gccaaatact
     7861 gagggacacc accccgcgcc tgttcccgct tcggcgcagg ttcaaatcgg ataccgttgt
     7921 ttttcagacg attaatcatt tcaggagttg tcttggtacc acgcaaaccc tttggcaatg
     7981 tgccgtcacc caaagactcg gaatacagca acatacctaa caaacccgcc cgtttaacta
     8041 acgctggatt attcatgagc ccgctaccgg cagattttcc ccatgacagc aacttaccgc
     8101 ctgccgctgt ggcacctgct gccacgccgc ctgcggaagc cttaccccat accaacgctt
     8161 tactacctac atctttcaga aagcctgcac ctttaccgcc accggcggtt aataacattg
     8221 cggcagacgc cgctgttgcc gccgtcgtca atgcttgcag ggctagagtg gcattagggt
     8281 attccgctgc cagcttttta aacgaggttt ccatatccac caatgttccc gtcagacttt
     8341 cccgcaacat tgccagcgat tcctgttgct cttgttgcgc cgctgccgac aacattcgcg
     8401 cctcgttgtt cttagcaatt tttccgttat ccaacgttac gccagccaac gaagccatat
     8461 attcacggat ttgcgtcata tccgttgcag ccagcaaacc ttgttttgcc tgcaaatcag
     8521 gcagcagttg cgccaaaagc gcgcccttaa gcatatttgc ctgctccgcc gccgtcttat
     8581 cgcctgcagc cgcgcgtttc ttataatctt ggtattgctt atcctttact agcatggcat
     8641 cggcaagacg ggacaacacc tgcactgcgt tttcgccgtt ttgcttgcct tgcacaaccg
     8701 agcctatcca atcgacacct ttcttcgggt catttggatt tgccatcttc ttcagacgac
     8761 ctatcgtgta aggcgacaga gttttactca aaagattttg cacattagtc gccgcttcgg
     8821 caggactgcc cgatttattc gccgcagatt gtaagagtga gagcaggtag tccaaaccgc
     8881 cgacaccatt catccctgcc tgttgcgcgg cagggagcag gctcggcagc tcccgaacca
     8941 tatcccgcac ctcgaaagtg ccgtctaaac ccgattgcaa gacgtgctca agcccgagtt
     9001 gcaggtcttt accgctcatg ccgccatctt tcagggtttt aatcagtttt gccgtatcct
     9061 cgccactgcc ttctgaggca agtgcaaaag catatgccgc ctgcgcttca ttcttggttt
     9121 gggcaaaatt cagactggtg gtcatcatgc cgctgattaa atccaaagcc ttatcgtggg
     9181 tcccgccatt tttctcgaca agttcaagtg ccaaatcctt gatctgttgc gcaccttcag
     9241 ttgcaatcca cgctgccgat ttactgttat cctcaataaa tgcctgtctg gacacgcggt
     9301 tgatgttctc atcaagctgc tttctgttgt ccatagcagg cttaagcacc gtatacgctg
     9361 ccgcgccacc agccaccgca gctgcaccga atcttccgat ttttcccatc ttgtccgcaa
     9421 atcccgtgcc ctgtttcagt tccgcgttca gctcgcgaat tcggttacgc gtagcgaccg
     9481 ccgcccgtgc caaatcattt tgtgatgcct tgccgctgcg cgccaaacgg ttaaacgcag
     9541 cctgtgtcag catgatttcg cgttgaatct gtttttctga gcggatgccg gtcaacgcat
     9601 aagtatggct ttgccgttca agttgcgccc gcgatttcgc ggtacgatct aattgtcgtt
     9661 gagtatcagc cagtagccgt ctcgctccgt catctcgggc ggttaacacc aacgacagtt
     9721 tcatgttccc gtttgccata aaaagcagcc tttaaacgta ttaactcaag gtcaattatc
     9781 gtttaaaggc tgtttaaggg aggcggtatg ggcttcaacc ccgttttcgt gctttatttg
     9841 tttgttgcgt tataatacca gaatgataac cattttattc aaagggatat tttatgaata
     9901 tgaaaaaatt gatttccgca atttgtgttt caattgtttt atcagcctgc aaccaacaat
     9961 caaaaacggc acaagccgaa gaacctgtcc aaagtatcca ggctgctgat tgtaccgccc
    10021 caatggacat cacagttgaa caatatctca tcaatttgga gcaagcattt aaaactcaga
    10081 acgtctcaac aaaaatccat aataaaaata ttgtcaagac cgattgtggt tatgacctta
    10141 ctttggtaat ggattttggg gcgattgcgc tcaaactgga cgagcagcaa aaaattagag
    10201 ctatctcagt aggctacatt ttaaaaaccg acggagagaa aggacaaaat ctagtcaata
    10261 atgccataaa tggattacac agtattcagg cagttctgtc tttaactacc acagacaaat
    10321 tgggcgaatc ggaagcagga aaacaacttc gtcaaagaat ccaatcagac aggagcaaca
    10381 gcgcaaaaag acgttccggc agatggtatt ttatatagcg ttgtttttga aaaagaaaca
    10441 aacaccattg caataatcgg cagaaaacaa ccctaaccgt caaataaaaa aaaggtcgtc
    10501 tgaaactcag acgacctttt tattttccct ccggctttct cagccgtttc gacacaatca
    10561 ctccctcttc cttcggcgtt tttatgccca ggctcgacag gatgtcttca aaccatgccg
    10621 acagctcggc gtggcacata tcgcgcactg cgtccgcccc tatccccgcc tttgccatca
    10681 ggatgaccgc ctgacggtag cttttgtgtg ccgtcaggta gctgcgtttt actgctccgc
    10741 cgccgacggc gcgtcggggc tcgccgtagc ggcggacggt tttaggcaca aatcctcctg
    10801 agccgccatc acggtatggt agtcttcggt agagaggttt tccatcagcc actgcgccgt
    10861 caggatgtcg gatgggatac cttgggcttc aatctgctgc gaccagtagg tcagcgtctc
    10921 taggacgtcg cgttgcagca gttcggcttt tgtggcgttt tcgggcagct cgggcaagtc
    10981 atcaatcagg gcgaacgccg ccagttcgcc gccgacggtc agtggcttga ggcgtaattc
    11041 agatacgact ttgccattgt aggtcagccc gtgggtcagt ttgacggtaa tcacgcatta
    11101 ctccttgatt ttgtgcaaag caatcatctc aatgtcgatg cgtgcctcgt tatcgacttc
    11161 atattgctcg ctggtatcga cggtaaagca gtcgaggtag gaagtgcggc gaccttcgtc
    11221 gttgatgggg taaatcgtga ttttcgcctt ggtgatgttg tcccagtcga tttccgtacc
    11281 gtcgatcgga atggcggcgg taacgcttaa tttgtgttcg gttacgccgt cacaataacc
    11341 gttgactcgg ccgttgcggt tcatcgtttt gaccggcttg cggcctgtag tggtttgcgg
    11401 tttgatgctc acaatctcga tatcgcggcc gttcacctcc ataatcaccg cgccggtata
    11461 ggtagcgtcg ctcatgcttt atcctttttt aaggtttcag acggccttta aacctgtttt
    11521 aaacgccgtc tgaaaagggt tacaaaatca aatcaatgcg cccggcaaag acgtgcaggc
    11581 cgttgaccac atcggcgggg ataatggcat taacacggtt ggggtcgttt tgcgcacgcg
    11641 ccaccaccag cttgcctttg ttggcctcgg cgttttcgat gatttcggct tggtcgagct
    11701 taatcagcac gtccaaaatc tcgctcttaa ccttgggcag caggcggtcg ctcaatttgt
    11761 cgcgcggaaa acgcagggca atgcgctctt taacgctgcg gcgcacataa tccagcgtgc
    11821 gaatggtggt aatgtcgagt agtgccgggt cgtcggtgtt gttggccgac ttggtatagg
    11881 ttgatacggc acgcataatc tgcacgcggt tgttgaccgc tgtgagcggg gtcaagccgt
    11941 tgtacagcgc attgttgcat tctgcaaacc acggccattg cgcatcaggt gtaacggcca
    12001 gccctttgat ttccagcgtg ttcagcggct tggcagggtc ttcttcaaag gccaacaccg
    12061 ccgcataacc tgcggcgatg atgccgtttg gctctaccgc acctttgtac caagcacagg
    12121 taatgcggcc gtcgttgatt ttgccggtag cggtggtggc cgtgctcaag gccgcactca
    12181 tacccaatac gccgatacag ccgcgctgct cgatggcgtt gaatacgttg gtaatatggt
    12241 tgctcaaggc tttggcgttg gcgtcatcgc taaacgggct gcaaataatg tgataatgct
    12301 tgcccgccac tttggacaag gccgtggcaa tgtccgcatt tttggcaccg ccggtaaaga
    12361 cattggcttg ataagtcagg ccggtattgc cggtgctcac ggttaagccg ctctcgttgc
    12421 cgatctcgcc tttgtgtttg gccgtcagcg taacggctac gccgctgccg gatgcggtaa
    12481 cggttacatc ggcggcagtg atggcggttt tcagacggcc tgccactgtg gcggcggtct
    12541 cgccggtgtt aacggccgtg cttacctgct tgccgccaat cgtgatttcc accacgcccg
    12601 gcgcggtggc cgtgccggaa agggtaacgg ttgcggttgc ctgcacgcct gcgctgtggt
    12661 cggcaatacc gataacggtc aaatccaaat aagggttgtt ggcaaatgct tggcgcacca
    12721 tcaaatgcgc cagcgagcct tgtccgaaca aatcggccgc ctcggcatcg ctaaatagtt
    12781 gcaccggctc taaggcgggc tgtatgcccg cggtcagcat gggtgcaacc atcaatacct
    12841 tttgcggatt ttgcggcaaa ccttgcacgg cattgcgggt gttaaattca atatactgcc
    12901 cgggcacgcg gatgctgccc ggaatcgtgt caaaatcaat atggggcatc attactctcc
    12961 cttagtattg cgggttttgg tatttggggc ggcatcctca accaccacca aatcgccgtc
    13021 atcaatcatg cggcggtaat acaggctgtt gccgtccacc tccaccggct cttggccgat
    13081 atattcgtgc gggttgtgtt cggtcggcac acgcaggcca tctgccgccg ttactttgat
    13141 tttgctcatt gcttatcctt aagggttagg tttacaggta tttcatcggc ggattgcggg
    13201 tcgtaaattt tgccgtccaa cccctcgaaa tcaggccacg gctcgctcaa tgtaccctga
    13261 tacttggtaa agatatggtt ggggtcgtcg ggattgtcgg tgcgttcggg gtagcggtca
    13321 ttttccaacc cgcaggtgtt aaagcggatg gcatactcaa ccgcatatat gcttactgcg
    13381 gcgttttgca ccagcacatg attggcaatc gcgcgcaccg ctttgggcac caagccgcgg
    13441 ctatcggcaa aaccgagccg ctggccgtca agcaggcggc gaacagcgcg gattaaatcg
    13501 ttgctgccga tttcgcggct gtcgatgccg ccttgccgct gcgcctgctc gttgcgcaga
    13561 ttgcgggccg ccaccatcac cacaaattcg gcggtatcct gataacgtcc gcatacgccg
    13621 ccggtgctgg caggctcaac tttgctgccg ccatacgtta cccaaaccgc aggcagcgta
    13681 tggatttgcc ccgccaaatc gtcggcctcg ccgttgtagc ttttaaccgt gcgcaccatg
    13741 cgacccaagc cccgtttcag acggtctgtt atcgcttgct caatatcagt gatcacgccc
    13801 gaacacctta tttttaccgt ttacaaacac aataccgtct tcgcccgcgg ccacttcttc
    13861 gccgctatca tccacgccca gctgcacttc gcctttggcc atagcacgca gcaggtcgag
    13921 cacgtcgatt ttgtagcggt tgcggatttc gtcggtaatc aacacgccct gagccgccgt
    13981 cagacggtag cgggcaatgt cgcagcaaag gcgcaccaag atgggcggca gatcctcaaa
    14041 aggtcgtctg aaccgcccca gatacgcgtc gatttcggca gtggcgtcca ccagcgcggt
    14101 ttgtgcgacc tcgcggtcaa tcagcccctc gttgttgcgg tcggtgagct gcaagacttc
    14161 cagctcaccg aaacgcgcaa ccatatcctc aaccgtcgcg tatgccatta ctcgaccgcc
    14221 ttgcgttgca gcataggctc ggcgcagatt gccttccaca ccgcttcgcc gacttcggcg
    14281 cgcttcactt cacgccagcc gccgtcaaac agcaggccgc cgcgccaaaa ttctttgccg
    14341 tctgcgccgg tactgacgag catcacatcg cggctgtccg ccaaagcgtc ggcggcgcgt
    14401 tgcgtatgct gcactttgag ttcggcaagt tcggcggaca gtgccttttt gtcgtcttcg
    14461 gctttttcca aggctgtggt cagcatttcg acatcgtttc gggcggcggc aagctctgcc
    14521 tgcacggcgt ccaattcggc tttgatgtct tcaaacgacg gggcggcggt ttcggcggtt
    14581 tctggtttgt tgttggtttt tgccatgatg actccttgtt tcagacggcc tttaaatagg
    14641 tgttaaaggt cgtctgaaaa cggttcgggg ttacaggtgg acgcaaacgt gcagtttcag
    14701 acggcctttg aaggtattgg tcgtgccgtt gattttgtcg gcttccaata attcacgggc
    14761 tttgtcttcc agctcgggcg gaacgaccaa gaggctgggc ttcacattca acacatagcc
    14821 accgtcggct ttgattttct gcattttgac gataatggcg gcgaggtttt cgcgggtcag
    14881 cgcggttttt tccgccatgt gcatcagttg ccacaaaccg aaaccggcat tgcagcggcg
    14941 gcgcgatccg tacaggtatt cgtcttccat aaacacgcgg tcggatttgg acgggtcgaa
    15001 tttggtttca aactcggagt cggtgcggtt ttggaaaatc agcggcttca gggtcttggt
    15061 gtcatcgacg acgtagaagg tcggcgcgtc gttgtccgta ccggtcgtca gattggagtg
    15121 cggcgtattc tgacccgtgc cgtccgattt ctcgtaaacg gggtggtcgg tatcgaagaa
    15181 atattggccg tcataaccga cagtggtttt accttttttg agcagacccc acaccagcgt
    15241 atcgggcagg gcggcggcgg actcgcccat tgccgccatc atcgggcggt acatgccgac
    15301 ttggtcgtct tcgatgtccg tacgcgctac gccgacggtt gcttcaaact ttttgttgtc
    15361 gaggctcatc gcctgcttgt tcattttttc gatttggcgc gagccgaccc attcgcgcat
    15421 ttgggggaat ttgccgagcc acgcataagt attggttgcc gtattggacg gaatggtcat
    15481 cgcaacggcg gtatagtcgg gcttgacgga ttccaaaccc gtttgaaatt ctttgcggaa
    15541 tgctgccgtg attgcggtca aaatcgctga tttgtccatt acttaccttc gctttctttg
    15601 atttttacaa attcttcgcc ggacatgccc agcatttttg ctgcggctgc ctcttccgca
    15661 gtcagtgcgg cgacgcgttc gtcgggtgct ttaccgcccg tttgcgagcc tgccagtgca
    15721 gcgaccggct gggcgttttc aataaagccg gtcaaaaatg ccaagccgcc cggctgtttc
    15781 aatacgcctt ctgcccactc cttctgagca ggcagcaatt tgcctgaagt cagcgcggcg
    15841 gtaatcaatt cgttgccttt gtctgcttcc tgcttggcag tcagcgcggc gactttgctt
    15901 tgcagctctt gaaccactga gataggcgcg tatttagtca aatcaggctt ggcggtttgc
    15961 gccgtcaatg ccgcgatgcg gctgtctttt tccgccagct gcgcgaacac gtcggcagac
    16021 aatgccacgt ctttcggctt ggcttccacg agcgcggaca atgccgcctt cagttcttct
    16081 tcgcccgcat caggcagacc gaacagttgc tgtaacaact ctttcatagg gttttgctcc
    16141 gtttccgatt ttaaaatttg cgccgacgct gccgccagca cctcgtccat accgtccaac
    16201 gcggggaaat ttgtcagcgc ggcgtgaaaa attttgctta catatccctt tgtgtcatag
    16261 gaaaacacag cagagatgta gcgatactct tttgcggcaa ttgccgcagc cgccttgtcc
    16321 gtccactcca cttcggcaaa catgccttta ggcgtgaact ccagccaacg catccaaccg
    16381 gcggcaggtg caggttgtcc gtttttctct ttgtagagcg tctggtgttc ataatcgaca
    16441 accaactgat tgcgcgagct gttggccaac aacgcgacat catgaccgtt ttcttcggtc
    16501 agataccacg cagggacatc agtcggacga ccgtctactg cgcgaaattc gccatatggc
    16561 agcagttgga ttcgcccgtc ttttggctgc acctcgaaac tgcacacggc aagtagggtt
    16621 ttttgtgcat ttttggacat gtgtacctct ctcaatacgg tcattttgcc tatttgaaaa
    16681 aacaacaaag agtaggcgtg cttcaaaccc tcccgccatt tttttaaaac ctgtccgcgc
    16741 gatacacaat taatcgtttt caggggcttt ttagaagcgt ctaggattga tttttatatc
    16801 ttgggcaggg gtttgtatta cccgcataaa aaaacgcgct aaaaagcgcg tttttgaggg
    16861 ttttataaat tcacggtatc agacccgaaa aataatcctg cacatcgtcc atcaaagcct
    16921 gtttgtcgtc gtccgtcagc gtcaaaaatt cccgttgcgg aatccgaact ttgcggttgc
    16981 gccccgccat accgccgaag ttgtggatgg cggcatagac gatattcgta ccgacaaggg
    17041 cggtatcgtt gtctgacagt gtggaaaaac tgtctttcag acgacccgaa tccgaaagcg
    17101 gcttgccgtc gcggtatttt agccccaacc atttcggacg gcctgcgtag cggaaattga
    17161 gcttgaccgc cgtgtgcatg gtttcggaca gtcggcgcat cagcaggtag cggttttcga
    17221 tcctgttgcc aagccgctcg atttggttta ggacgacaaa gatattgtct attttgacat
    17281 caatcatggt attatcctgc ctaggctact aaaaaagcgg tgaatttgtc tgccctccgc
    17341 agcccttatg cagtcctcgg actgggattg gtatccgtta cataagcgag agtgtgtcgg
    17401 gattgcagtc cgacttagta gccttttttc atttcatagc gtttcttact tttcacaatt
    17461 tctttttcat ccgccctgcg aaacgaatcc aaaaaaatcg aatcgtactg tggcagatat
    17521 ttcaaaactg ccatcaatct gtatttttcg ttcccctcca acggttttcc gagcaaaaca
    17581 aaactttctt ttcctctgct tttgtcataa aaaatatatt cgggctcatg aatcagatcg
    17641 gggagcatgg catataaatc tgttccaaac gattgcccct cccgactgtc cacctgtttg
    17701 accaatgtat catcagacag ccacaccgtc gctcgtgtca tacctgccaa ttcttgcgtt
    17761 tccttgctca atacacccgc agcaaattta agctgtcttg atagcgcatt tcggattttg
    17821 attttctgct ctttatcggg cttgccatca atatccaaac gttgcttgac ttcataaaac
    17881 tctttttcaa gctgtttaaa gctggtttta aaatccgcac cacccatttc cgctttggca
    17941 aattgatgcg ccaacgcacg gtcgtacttg tctaaatcgg ggcggtagtt cattcgtccg
    18001 gcgttgtaat caaatcctcg atcggtcgtg tacagactgc catccggtgc tttataggca
    18061 agggtcagat aagtatcgcc ttttttgttg tagattttat gggtctcgac tagattgtcc
    18121 gccgtgcttt gcccgacaat ccgcccctgg cgttccacat cccgctccga cagcgcgatg
    18181 accgagcaac ggcagttgta gccgttgggc gggtaaaacg ttgcccaaaa cgggtcgtcg
    18241 tagcggtaca ccagcccgtc tattgccgaa tgcgccggac gggtgcggct gtcgcctacc
    18301 gcgtcataca tccaataggg ccgtgcatca atatttgcca tatatccttg atattgaccg
    18361 gcgttgtagg cagtttgcat gttggtacgg taaatcgtct ccaacctccg cggcgaaccg
    18421 aatacctcgc cggtggctgg gtcgatgata tccttaccgt tatgcccgtt cggatgcagc
    18481 cagcccttgt tgccgagcag attcaagata ccttttcgcc aatcgtcaaa cgactgtcca
    18541 ctttttgccg attcaaccat cgaagttttg atgtcgttga gcatatccaa gtcggtcatg
    18601 cgggcaatcg tatagacttt ggcaatttcg gaggctgtca gattgcggta gctctccgcc
    18661 gtaacctttt tactttccag ccactcgatt gccttttttg gcggcagact taaggcaaat
    18721 cccaaatcag gcgcgggcat ggtcttgtcc caaaatatcg ctgataaaca aggcctgctg
    18781 catataggta cgcagtttcg cgttgtccaa attcggataa agcgcattca gtgcggcatc
    18841 tgcctcctcg tagctgttgc aagcattaag tgcggcaacc gcctgacgca ccatcgggtt
    18901 gagctgagaa ttgaaatcgg gctcaaccag cgcgtcatcc aacgcgccgt ccaatatttc
    18961 ctgatgcctg cccgtagcct tagatggtac ggtgtgggcg gataaagccg ccaatgcagt
    19021 tctgtttatc ggattgtccg gtacctgccg caccaacaca gcctcaccct cctgcacatc
    19081 tggaatgacc agtttgtcgc gcacccagct ttcggggatt tgtacgccga catccaccag
    19141 tttcgggata gcgtcggcaa agaccgcgat gtctttcggc tcgcgcgtgt caaattcaaa
    19201 tttcggcacg cggtttgggt cggcattggg atagttgatt tgcaggaacg gtccgatgat
    19261 ttggcttgtg atggtttgcg ccacctgttt tgcgtccgac accagcaaat cgcggcgtac
    19321 ctcgttgtgg atattgccca gcgcgttggt gctggatttt ccgtccgcac cgctggttag
    19381 cgtttgcccc aaaatcagcc gcgccgccga tttttcgcac cagtcggcca tctgcaaaaa
    19441 cggattgctg gttgccatcg taccgcttgc agcgttgtgc agctcgattt ccataccttc
    19501 tggcatgatg cctgccgcgt tgtgaccgat ttccgccacc gctcgaagca gggtgttttt
    19561 ttcctctttg gttgcgcccg cgccgtattt gccgatacgg atgggcatgc cgtacagctc
    19621 caaaaactcg gcaaaatcgt ggacggcgta gtgtttgaac atatacagcc aggaaagcgt
    19681 gcggaaaagc ccgttgcgcg cctgctggac gctgcgcgat ttttgggtat gaacgaccca
    19741 gcccagcggc cacaacgctt cgccttccgg attttcgcgg gtacgcagca gcagcccgtt
    19801 gtctttgtcc catttgaacc agctttgcgg gcggtggata aagtttcggg gtaggtaaag
    19861 gccgtctgaa aatacccact cgacctccaa cgcagaaaat ccgtgcccta ccgcgtccat
    19921 caaatccata atcaggtctt cgagggtagg caggctgtcc atcatttcgt aggcttggtc
    19981 ggacagcttt tcttcttcgg gcgtcgcatt tcgcggcggg gcgacgcgcc agttgagcgt
    20041 cagcagcgcg cgtttgcgcg tccccatatt tgccgcgatg tcgctgtcgc gctcctcaat
    20101 gtccgcgaaa agctcgtgtt gggcgcggat gtcgccgctt tccgcatcct cgaagagggc
    20161 ccgcatcttt tgcggtgtaa taaaattgga cggatgctcg gcgataaccc gaccggtcgc
    20221 cgtaatttga gccacgtccg tctgcaatgc agcttcgggc ttttggattt tagttttatt
    20281 gttctttttt gccattatcg gctccattta ctttttatct cgtcatcaaa atcgttatcg
    20341 gtagggcttt gccattctat cggcacacaa ttggccaccg cccccgacca gagcatatgc
    20401 actgcatcag gaccatcatc atgatcagcc tttggaaaat ggcggaactg ctgtatcagc
    20461 gtctgttggc tctcattcaa cagaatcaaa ccgttcgcca tgtgaggctg taaagtctca
    20521 atccgcaaga gcttgtccga taccggcttg accgcccgcg ccgggacagg tattccacgc
    20581 gccgcgctgc gcttgaccag ctcatctttc aaaaattcct gaaactgaac cgtctcaaca
    20641 aaccacagtt tgcaacgata ttgacggtgc aatctgataa cgtcctcaat aatcaaatca
    20701 gggagacgtt ttttaatctg agcttccacg acatacagtt tgccggtttc acgttgataa
    20761 ccgccaatga taatcgccga cgggtcacgg cttgcccccg ctttacccag cgacgggtcg
    20821 agcgcaccga aatacaccaa atcggacggc aggtcgttcc aaaacttcat cgacttggca
    20881 aacggcgcat cttcgccgct gaccgggtcg ttttgatact cgctgtcaaa tgtcgcatga
    20941 ccgtcacggg cgcggatttt catcagcgcg agtacgccgc gagccgccca gcttgtttgc
    21001 gcgccgcgct ccatctcgtc tttattggcg agataaaacg cctcggccac cgtctcgccg
    21061 tcgtttcgga aaagttcctc ccatctgtcc cacaagtcca tgcggtcggg ccagcgtttc
    21121 atcgccttaa acttaacacc ccgccaaaac ggattattca gggtgcggtt tagcacgctg
    21181 tcgtaatgca aaatcgtgcc gatataaatc acatcgtatt tctgaccgac accgcccaaa
    21241 ggcaatacag tcttagtcag ccacgcattg agcttgtcgc gctgttcggg gttgcggact
    21301 tgctcgtcat tctcaatatc gtccaatatg gtcaggtcag gacggtaagg gccgtgacgc
    21361 aaaccgcgca gctttttacc gctaccggcc acttggactt taacgtcatt ggccgtcgca
    21421 atcgtaccgg cctgccatac gcggccctgc ccgcatactt ccggaaagtc ggttttcaag
    21481 cgcggattaa attccaattc cgccttgatg gcttcgagca tcgggtatgc ctgatctatg
    21541 ctgtccatca caataacggc ataatgtttt tggcctgtca caatacacca cagcgtaaac
    21601 aactgagtaa cctgcgtcga cttaccctcg ccacgcggcg cacccaccgc ctcattttcc
    21661 cctttggggg agcggataat ctccggcaga cggctgaata aaaacgcatg cagttcggat
    21721 ttttcagggg agcggatata atgcggaaag taggtgttga cgaaatactc gtaaccgcct
    21781 accgggtcaa acaccttggc acggcgtgca gcaatagcct tgggcaacgc atcaaagccg
    21841 tccacttccg cttcgatgac ttggcgcaaa ctggcggcca gttcggcaag ggatttgagg
    21901 aattcttttg ttttcatgag tatttataat gactggattc atttctaacg catttatagg
    21961 cctaaaagaa gcaggatcac ttttaaaagg gtttaaccag ctcaaaacag aggcagaggt
    22021 agcgcaaaaa accatcgaac ttaatcaaat cattgctggt attcaaactg atttatttga
    22081 agcccaatct tcttatacgg cggcaattag ccgtatagat gagttggaaa aagaactggt
    22141 ggcagtaaaa aattggtcta cagagaagca acgctaccaa ttgtatgaac ttgctcctgg
    22201 ttctttcgtc tatcgattga aaacagagat ggcgaacagc gagccaattc atgatatttg
    22261 tccccaatgt tacgagcagg gtgtcaaatc gattcttcaa ttcaatgggt atgaaggtgc
    22321 ttttcataaa tattcctgtt cccgttgcca aaccataata ttgggagagc gtttaaagtc
    22381 agatgttgaa gtattttcag taggaggatc acgttatggg agattgatag acggcta
//
LOCUS       DAWHXS010000024        22333 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWHXS010000024 DAWHXS010000000
VERSION     DAWHXS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.024"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1166)
                     /locus_tag="WIE22_000694"
     CDS             complement(<1..1166)
                     /locus_tag="WIE22_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ4126825.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
     gene            complement(1223..3748)
                     /gene="ligA"
                     /locus_tag="WIE22_000695"
     CDS             complement(1223..3748)
                     /gene="ligA"
                     /locus_tag="WIE22_000695"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ4126826.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(3896..5182)
                     /locus_tag="WIE22_000696"
     CDS             complement(3896..5182)
                     /locus_tag="WIE22_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ4126827.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5376..5948)
                     /gene="ampD"
                     /locus_tag="WIE22_000697"
     CDS             complement(5376..5948)
                     /gene="ampD"
                     /locus_tag="WIE22_000697"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ4126828.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            6032..7027
                     /gene="mltG"
                     /locus_tag="WIE22_000698"
     CDS             6032..7027
                     /gene="mltG"
                     /locus_tag="WIE22_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ4126829.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7087..7707
                     /locus_tag="WIE22_000699"
     CDS             7087..7707
                     /locus_tag="WIE22_000699"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ4126830.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            7929..9209
                     /locus_tag="WIE22_000700"
     CDS             7929..9209
                     /locus_tag="WIE22_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ4126831.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            9445..9716
                     /locus_tag="WIE22_000701"
                     /pseudo
     CDS             9445..9716
                     /locus_tag="WIE22_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9756..10790
                     /locus_tag="WIE22_000702"
     CDS             9756..10790
                     /locus_tag="WIE22_000702"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ4126832.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10996..11577
                     /locus_tag="WIE22_000703"
     CDS             10996..11577
                     /locus_tag="WIE22_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ4126833.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11646..11828
                     /locus_tag="WIE22_000704"
     CDS             11646..11828
                     /locus_tag="WIE22_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ4126834.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            11825..12586
                     /gene="kdsB"
                     /locus_tag="WIE22_000705"
     CDS             11825..12586
                     /gene="kdsB"
                     /locus_tag="WIE22_000705"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ4126835.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            12609..12926
                     /locus_tag="WIE22_000706"
                     /pseudo
     CDS             12609..12926
                     /locus_tag="WIE22_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            13031..13228
                     /locus_tag="WIE22_000707"
     CDS             13031..13228
                     /locus_tag="WIE22_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126836.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            13385..13477
                     /locus_tag="WIE22_000708"
     tRNA            13385..13477
                     /locus_tag="WIE22_000708"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13419..13421,aa:Ser,seq:gct)
     gene            13780..14565
                     /locus_tag="WIE22_000709"
     CDS             13780..14565
                     /locus_tag="WIE22_000709"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ4126837.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            14602..15474
                     /locus_tag="WIE22_000710"
     CDS             14602..15474
                     /locus_tag="WIE22_000710"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ4126838.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(15534..16145)
                     /locus_tag="WIE22_000711"
     CDS             complement(15534..16145)
                     /locus_tag="WIE22_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ4126839.1"
                     /translation="MRRTALLLLALTAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            16253..16477
                     /locus_tag="WIE22_000712"
     CDS             16253..16477
                     /locus_tag="WIE22_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ4126840.1"
                     /translation="MNSETLDVIGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            16478..17083
                     /locus_tag="WIE22_000713"
     CDS             16478..17083
                     /locus_tag="WIE22_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126841.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            17144..18178
                     /gene="pyrC"
                     /locus_tag="WIE22_000714"
     CDS             17144..18178
                     /gene="pyrC"
                     /locus_tag="WIE22_000714"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ4126842.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKACIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENVCGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(18544..18969)
                     /gene="nusB"
                     /locus_tag="WIE22_000715"
     CDS             complement(18544..18969)
                     /gene="nusB"
                     /locus_tag="WIE22_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ4126843.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(19047..19523)
                     /locus_tag="WIE22_000716"
     CDS             complement(19047..19523)
                     /locus_tag="WIE22_000716"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ4126844.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(19571..19894)
                     /locus_tag="WIE22_000717"
     CDS             complement(19571..19894)
                     /locus_tag="WIE22_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ4126845.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            20061..20780
                     /gene="rnc"
                     /locus_tag="WIE22_000718"
     CDS             20061..20780
                     /gene="rnc"
                     /locus_tag="WIE22_000718"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ4126846.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            20828..21751
                     /gene="era"
                     /locus_tag="WIE22_000719"
     CDS             20828..21751
                     /gene="era"
                     /locus_tag="WIE22_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ4126847.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         4951 a         5482 c         6587 g         5313 t
ORIGIN      
        1 cactgcagca aatcgtttaa aaacaagcgt cctttttcgg tcgggcggaa tacggcaggg
       61 tcggtttcca gcaggccttt ttgccttgcc gtttcgattt gcgccatgat tttggcactc
      121 ggtacgcccg tgcgctcctg caacatcgcg gcgggtacgc cgtcggtcag gcgcagggcg
      181 ttcatcatga attcgaacgg caaatcttcg gcagcgacgg ttttgcgttc gacggcttca
      241 ctcggttggc tttgcattaa ggcgaggtag tcgttggggt ggcggcggcg gacggtgcgc
      301 tcgatgcggt cgggatagga aattttgccg tgcgcgcccg cgcctatgcc taaataatcg
      361 ccgaactgcc agtagttcaa attgtggcgg cactgcatgg ctggtttcgc aaaagccgat
      421 gtttcgtagt ggacaaaacc cgcgccttcc agcgcgccgt gtaccgcgtc ttcgatgtcg
      481 agggcggctt cgtcttgcgg caaacctttc ggcggcgtat gaccgaacgg cgtgttcggt
      541 tccatcgtca gatgatacgc gctgatgtgg gtcgcgcccg tagcgatagc ggtttgtacg
      601 tcgtccaatg ccgtctgaac ggtttggttc ggcagggcat acatcaagtc gatattgact
      661 ttatcaaata atttcaaggc ggtatcgata gcggttaagg cttccttacc gttgtggacg
      721 cgccccagcc ttgagagcat atcgtcgttg aaactctgta cgccgataga aagccgcgta
      781 atacccgcgt ctttaaatcc ttgaaacttc tcgatttcaa atgtccccgg attggcttcc
      841 aaggtaattt ccgcttccgg ctgcaagcgc aacagcgaac gcacgccgct taacaaacgg
      901 tcaatcgatt ccgcctgaaa caggctgggc gtaccgccgc cgaaaaagat cgtttccacc
      961 ggcctgcccc aaatattggg caattcaagc tgcaaatcgg tcagcagcgc gtcgatatag
     1021 gcggcttcgg gcaatccgtt tttcaggctg tgggaattga agtcgcaata cgggcatttt
     1081 ttgatgcacc acgggatgtg gatgtaaagc gacaggggcg gcagggcggt gagtcgggtg
     1141 cggtttggaa aggaaatggt gtgcatggtg tggttcggaa aagtgggcaa tgccgtctga
     1201 aggcggttca gacggcatgg gttcagccga gcagggtaag cagttcggct tcgctgagga
     1261 cggaaacgcc caaggcattg gctttttcca gcttgctgcc cgcggcttct ccggcgacga
     1321 cgtaatcggt ttttttggac acgctgccgg aaactttgcc gcctgcggct tcgattaggg
     1381 attgggcttg gtcgcgtttg agggtgggca gtgtgccagt taacacgaag gttttgcccg
     1441 ctacggcttt attgatgccg tctgaacctt gcgccgcctc gtcttcagac ggcatttgcg
     1501 cgaagaaggt tttcaggttt tcgagtaggg cggcgttttg tggttcgctg cgccatgctt
     1561 gccagtcggc ggggagggat ttgtcggttt gcagaccctc tatacttttg ccggcgagtt
     1621 cccataaggc ttgggctttg ttttcgctaa ttttgaaacc gggcaggcgg gcaatccagc
     1681 gttgcggttc ggcatattgt gcggcaggca ggctgacggc ttgggcttgc ggggcaacgc
     1741 ctgcggcgag cagctcgtct atcatcgcct gctgttcggc ttgggcgaag aagtgggcaa
     1801 tggaacgcgc cactaccgta ccgatgtcgg gcaggcaggc gaggacgggt tcgggggcgc
     1861 ggcggacgcg ttccaatgtg ccgaatgcct gtgccagcgt tttggcggtg cgttcgccga
     1921 cgtggcggat gccgagcgcg aacaggaagc gcgcgagttc gggcgttttg ccggattcta
     1981 tgcctgcgag gatgttttcc gcccacttgg tcggttgttt tttatgtttg cccgacgcac
     2041 cgaccgaact gccttcagac ggcatttgat ccgattcggc aacggttttg tccgccgttt
     2101 ccttcatttt ttgcagggtc ggaatatcga ggcggtagag gtcggcgaag tggcggacga
     2161 ggtcttgggc gacaagctgt tcgatttgtt tttcacccaa gccgtcgatg tccatggctt
     2221 tgcgcgaggc gaagtggatt aagccttgcg cgcgttgtgc ctgacaaagc ataccgccgc
     2281 tgcatcgggc gacggcttcg ccttcttcgc gttcgatttc gctgcggcag atggggcaat
     2341 gggtcggcag gcggtagggc ttgtagagcg gaacggattg ggtttggttt gcagccggtg
     2401 cttcggcaaa caaatcgtcc tgccgatgcc cgatgccgtc tgaaacagca acggcggttt
     2461 cctgcatcgg gcggcgttca aaaatcacac gcacgacttc gggaatcacg tctccggcgc
     2521 ggcgcacgac gacggtatcg ccgacgcgca cgtcttttcg ggaaacttcg tcctgattgt
     2581 gcagggtggc gttggtaacg gttacgccgc cgacgaatac gggttgtaat cgggcaaccg
     2641 gcgttaccgc gcccgtcctg ccgatttgca cgtcaatcgc ttcgacgacg gtcagggctt
     2701 cttcggcagg gaacttgtgg gcaatcgccc agcgcggcgc gcgggagatg aagccgagtt
     2761 cgcgctgttg cgccaggctg ttgactttaa ccaccatgcc gtcgatttcg tagggcagtt
     2821 cggggcgttt ttgctgcatg tgttcgtaaa acgccaatac ttcgtcgata tttttgaaac
     2881 agccgaaatt gccattgggc agactgaagc cgagtgcttg gaaataggcg agttcttgga
     2941 tgtgttcttc cgcgacgaaa ccgccttgcc ggcgggcgac ggagtagggg aaaaagtgca
     3001 gtttgcgttg cgcagtgatg cgcgaatcga gttggcgtag gctgccggcg gcggcgttgc
     3061 gcggattggc aaagggtttt tgcccgtttt cggcttgtct tttattgagg gcggcaaaat
     3121 cggctttttg catcagtact tcgccgcgta cttcgatgag ttcgggcgta ttttcgccgt
     3181 gcagccgcaa ggggatgttg gatacggttt tgatgttttg ggtaacgtct tcgcccgtcg
     3241 tgccgtcgcc gcgcgttgcc gcctgcacca atacgccgtc gcggtagagc aggctgatgg
     3301 cgaggccgtc gaatttgggt tcgataacgt attcgggatt gccgccgtcc aagccgtcgc
     3361 gcacgcgttg gtcgaaggcg tacatttcgg catggtcgaa cacgccgttt tcatcttgcg
     3421 gggaaaaagc gttggtcagc gacagcatcg gcacttcgtg gcgtacttcg gcaaatcccg
     3481 ccaaaggctc gccgccgacg cgctgggtcg ggctgtcggg cagtttgagc tcgggatggt
     3541 ttaactccaa cgcttcgagt tcgcggaaca atttgtcgta ttcggcatcg ggtacgctgg
     3601 gcgcgtcgag ggtgtagtat tcgtaggcgt agcggttgag gaggtcggtg aggcggcaga
     3661 tgtgttgtgc ggcaaattgt tttatatcac tatcagacgg tttaagaaga ttggtaaagt
     3721 tagtgttatg ttttgagttt ggattcatga gagaaggttt tcagacgacc tttgtctgat
     3781 acgggatgaa acgggcaaag gtcgtctgaa aaatgatagg ttgaaaacag ctgaatttta
     3841 cccgaaaaaa agcggatatg ccgtaacgac atatccgctt tgattgcatt cgattttagg
     3901 agaacaggcg caatgcggtt ttgccgcccg gttcgatacc gactttgagc atctcggact
     3961 gacgcgccaa tacataagtg cgcacgtctt tgagccattg ggtcgaaact tcttccattt
     4021 tgtcgttgac cagattcagg ttcaactggc cggacaggcg taccgccaaa tccataaaca
     4081 aatcgtcgaa ggttttttcg cctgccggag agtgcgggat gtcgagcagc atactgaagc
     4141 ctttgtagga ctggttgtcc aaaagggcgt tggtaaacgg ctcgttgttg agcgagcaga
     4201 tggagaacat ggtcgagccc gacgtgtcgg tatagtggaa cgcgccgtcg tcttccaaaa
     4261 cgaaacccac gcccgttacg gcggaacgca gttctacgcc gctgatgctg gtcggggaaa
     4321 ccaaatggat ggcgatggtc tggtcgacgc gcgcgcagaa tgcgtccagt gcggaagcca
     4381 cttcgataaa ggcggcaagg tcggtgtgca gcgtctgacc gcccatgctt tgtgcgaatg
     4441 cgtccacctg gcggttgaat gcggagagtt cttcctgcga ggcaagtccg ttgcggctga
     4501 ctgcctgaat acccacgata aatgcctgat agcggatgcc cgggatgggt tcggcaatct
     4561 ggaaatggtc gtccatggtg cagccgacaa tctggtagcg gcagcggttg gaaaggcgcg
     4621 gcagtgcgtg cagttctttg gcttcggtca gcgcgatata ggagatgaag tcgaagcgca
     4681 cgtcaaacca gggtaattcg acttttgaca gttctttgag cgtaatcagc ggttttgcag
     4741 gtgtttgcgg aacgggtgca ggttttgccg gcgcgtcggc aggtttcggt gcggaatgtc
     4801 cggtttgggg ttcggaaacg gtgtgggcgg agttgccgat aatgccgctt tcttccaagg
     4861 cggtttcgat ttcggttttg aacggggagg cttttgcctg tttctgcttg gcgatgtaga
     4921 cggcatcctg ttcttgcagg ttgcgcatag cagggtcttg gagttttgcc gtttttttga
     4981 ccgccggttg gggtttcggc atcatgactg acccgccgga cggtttgccg tcgcggacat
     5041 ggctggtttt gctgttgagc agggcatctt tgtcggagtg tccgaactgg tcgcgcactt
     5101 ttttgcggta ttggttttcc tgatacatat tgtaggcgac aacggcgagg acggcggcga
     5161 ggaacagtac gatgtaaatc atggcaatca ctttaaattt cgggatgcag gatgcgcgaa
     5221 gtgcgggtac tgcggttaaa tcgggcttgc gtttccggca gtctgatgga acggccggtt
     5281 ataacgtttg aattataacg aaaattgcag ggtctgacag cagtgtgtcg aaataagcgg
     5341 aaattttccg aaatgccgtc taaaatctgt ggttttcaga cggcatttct gtccacggga
     5401 aaccctttcc cccgtatccg ccgccagtcg aaaaaatgac cggggtcggt tttgcggccg
     5461 ggcgcgatgt cttggtgccc cgttaccgcc gtgatggggt agcggcggca gattgcgtcc
     5521 aacaaggctt cgagcgaacg gtattgcgct tcggtaaagg gttcgaaatc gcagccttcc
     5581 agttcgatgc cgatggaaaa tgcgttgcat ttttcccgtc cgcgaaacga ggatacgccg
     5641 gcatggtatg ccatattgtc gcaggaaacg aactgtaccg tttcgccgtc gcgtttgatt
     5701 aaaaaatggc tggatacgcg caaagtgtgt atcaggctga agaacggatg tccgtcgggg
     5761 tcgagccggt tggcaaacag cttttccacc gcatccgtac catattcgaa cggcggcagc
     5821 gaaatgttgt gcaacacgat cagggaaacc gtttcccccg tttccctcgg gctgaaattg
     5881 ggcgacgggg tatggcgtat gctttgaagc cagccgtttt gccagtgtgc ttcggcgtga
     5941 ttgtccatga tgttcttcct gtccggcggg caatttgggt tatactgtcg cccgaatttt
     6001 aagacgtatt ccgaatgctg ggaatcctac catgttgaga aaattgttga aatggtctgc
     6061 cgtttttttg accgtatcgg cagccgtttt cgccgcgctg cttttcgtcc ctaaagacaa
     6121 cggcagggca tacaggatta aaattgccaa aaaccagggt atttcgtcgg tcggcaggaa
     6181 acttgccgaa gaccgcatcg tgttcagcag gcatgttttg acggcggcgg cctacgtttt
     6241 gggtgtgcac aacaggctgc atacggggac gtacagactg ccttcggaag tgtctgcttg
     6301 ggatatcttg cagaaaatgc gcggcggcag gccggattcc gttaccgtgc agattatcga
     6361 aggttcgcgt ttttcgcata tgaggaaagt catcgacgca acgcccgaca tcgaacacga
     6421 caccaaaggc tggagcaatg aaaaactgat ggcggaagtt gcccctgatg ccttcagcgg
     6481 caatcctgaa gggcagtttt tccccgacag ctacgaaatc gatgcgggcg gcagcgattt
     6541 acggatttac caaatcgcct acaaggcgat gcaacgccga ctgaatgagg catgggaaag
     6601 caggcaggac gggctgcctt ataaaaaccc ttatgaaatg ctgattatgg cgagcctgat
     6661 cgaaaaggaa acagggcatg aagccgaccg cgaccatgtc gcttccgtct tcgtcaaccg
     6721 cctgaaaatc ggtatgcgcc tgcaaaccga cccgtccgtg atttacggca tgggtgcggc
     6781 atacaagggc aaaatccgta aagccgacct gcgccgcgac acgccgtaca acacctacac
     6841 gcgcggcggt ctgccgccaa ccccgatcgc gctgcccggc aaggcggcac tcgatgccgc
     6901 cgcccatccg tccggtgaaa aatacctgta tttcgtgtcc aaaatggacg gtacgggctt
     6961 gagccagttc agccatgatt tgaccgaaca caacgccgcc gttcgcaaat atattttgaa
     7021 aaaataaacc atgccgtctg aaaagtttgt gttttcggac ggcataccct taccggaact
     7081 gcaagcatga aaccgcaatt catcactttg gacggcatag acggtgccgg caaatccacc
     7141 aaccttgccg tcatcaaggc atggtttgaa cggagggggc tgcccgtgct gttcacgcgc
     7201 gaaccgggcg gaacgccggt cggtgaggcc ttgcgcgaaa tcctgctcaa ccctgaaacc
     7261 aaagccggtt tgcgtgcgga aacactgatg atgttcgccg ccagaatgca gcatattgaa
     7321 gacgtgatat tgcctgcact ttcagacggc atccatgtcg tgtccgaccg ttttaccgac
     7381 gcaaccttcg cctatcaggg cggcgggcgg gggatgccgt ctgaagacat tgaaattttg
     7441 gaacattggg tgcagggcgg tttgcgcccc gatttgaccc tgctgttgga tgtgccgctg
     7501 gaagtgtcga tggcgcgtat cggacagtcg cgcgagaaag accggttcga gcaggaacag
     7561 gcagatttct tcatgcgtgt gcgcagcgtt tatctgaacc gtgccgccgc ctgtcccgaa
     7621 cggtacgcca ttatcgacag taaccgcagc ttggatgaag tcagaaacaa catagaaaaa
     7681 gtgttggacg gacatttcgg ctgttaatgc ggcaaatatt gaaacaagcg catccacccg
     7741 cgccgaaaat caaacggcag tgccgcaggt gaaaatggcg gtatgcgcca aactttcggc
     7801 atgatagaat tacgctcggt tacaaggcag gatgcgtcgg caatattaac gaaccgcccg
     7861 taacatgatg acccgaaagc gtttcggaca gtccgattca aatctttttc tcgcaacagg
     7921 attgacacat ggaaaactca ttgaaagaag ccgccctcaa gttccacgaa ttacccgttc
     7981 cgggcaaaat ttccgttacc ccgaccaaat ctttggcgac cgacaaagat ttggcgttgg
     8041 cgtactctcc gggcgtagcc gctccttgta tggaaatcca tgccgatccg caaaatgcct
     8101 acaaatacac cgccaaaggc aacttggtcg ccgtcatttc caacggtacg gccgttttgg
     8161 gcttgggcga catcggcgcg ctggcgggca aacccgtgat ggaaggtaaa ggcgtattgt
     8221 tcaaaaaatt cgccggtgtg gacgtgttcg acatcgaaat cgatgaaaaa gacccgcaaa
     8281 aactcgtgga catcatcgcc gctttagagc cgaccttcgg cggcatcaac ctcgaagaca
     8341 tcaaagcacc cgagtgtttc tacatcgaac gcgaattacg caaacgctgc aaaatccccg
     8401 tattccacga cgaccagcac ggcacggcca tcattaccgc cgccgccgta ttgaacgccc
     8461 tgcgttttac cggccgtaaa atcgaagaag cgactttggt gtgttccggc gcaggtgcgg
     8521 ccgcgattgc ctgcttgaac caattgctgg atttgggctt gaaacgcgaa aacgtgaccg
     8581 tttgcgactc caaaggcgtg atttaccaaa cccgcgaaga caaagaccgc atggacgaat
     8641 ccaaaaaatt ctacgccgtt gaagacaatg gccagcgcgt acttgccgat gccgtcaaag
     8701 gtaaagacat cttcttgggc ctctccggcg cgaacctgct gacccccgaa atgttgaaca
     8761 cgatgaacga aaaacccatc gtgttcgcca tggccaaccc gaatccggaa atcctgccgc
     8821 cgctggcgaa agaaacccgt ccggacgtgg ttatcggtac cggccgctcc gacttcccga
     8881 accaagtgaa caatgtattg tgcttcccgt tcatcttccg cggtgcgttg gatgtcggcg
     8941 cgacgaccat caacgaagaa atgaaacgcg cctgcgtgta tgctttggcg gatttggcga
     9001 tggaagaagt aaccgaagaa gtggttgccg cttacggtaa gaaatttgaa ttcggcgcgg
     9061 aatacctgat tccgactccg ttcgattccc gcctgctgcc gcgcgttgct acggctgccg
     9121 ccaaagcagc gatggaaagc ggcgtggcaa cccgtccgat tgcagatttg gaagcttacg
     9181 ctgccaagct gagcgaatgg aagctgtaag ccgtttgcgg tttaaaatgc cgtctgaact
     9241 gttttcaggc ggcattttgc tgtcagattg atatagtgga ttaacaaaaa tcaggacaaa
     9301 gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc
     9361 ttagagaatc gttctctttg agctaaggcg aggcaacgct gtactggttt ttgttaatcc
     9421 actataaatg aaagatactg gaaaatgaaa gagatgaaac ctgtccgtta tcatattggc
     9481 gatatgcccg agacttcaaa acaaaccgcc tcccgtcatg acgacagggc agtgggtgtt
     9541 gacgatgatt gttttcatga ttcctttggt caatttttgt ttgggtgttc ggcagaggca
     9601 acccgaaccg cgccaatttc tgtaaagcgc agttgcttat ttacctgatt ggttcgctta
     9661 tcggtttggt cttcgcgttg tttataggtg ggtctgtatc gggtacgcat gattaatgcc
     9721 ccgggctgat tttgcttcga ggatttgtat cgaatatgcc gaattgtttc aaatttcata
     9781 ctgttatcga acggcattgg caaaaacctt atccggtttt gtcttttctg cttaagccgc
     9841 tctccgggct gtttgccaaa attgcggcaa aacggcggac ggatttttta tcgggaaaac
     9901 ggcaaagcga aaagctgccc gtgcctgtgg tcgtggtcgg caatattcac gcgggtggga
     9961 cggggaaaac gccgattgtt gccgcgctgg tgtcgggttt gcaggaaaag ggcgtcaagg
    10021 tcggcatcat cagccggggc tacgggcgca agagcaaggc ggttcatgta ttgaatgctg
    10081 agagccgagc ggaagatgcg ggcgatgagc ctttgctgct gttccgcaaa accggtgcgc
    10141 cgacggcggt gggcagcagc cgtgcagagg caggcagggc gttgctggcg gcgcatcccg
    10201 acatcggact gattgtggcg gacgacggtt tgcagcatta cgccctgcgg cgagatgtgg
    10261 aaatcgcggt gtttccggcg gcggatacgg ggcgcacgga tttggattta ctgcccaacg
    10321 gcagtttgcg cgaacctttg ttgcggctgg attcggtgga tgcggtcgtc gtcagcggcg
    10381 gcaaggcgga tgcgctgttt aggccgtctg aaaatatgtt tcacagccgt atcgaagcgg
    10441 gacggattta ccgtttgaac aatccgtccg aaatactgga cacaggccgt ctgaaaaatc
    10501 aaaccgtcgt cgccgtggca ggtattgcca agccggcgcg ttttttcgat tcgttgcgga
    10561 atatgggcat taccgtgaag cgaaccgtcg cgctgcccga ccacgccgac atttcggcgg
    10621 cagatttgcc cgatgcggac gcggtcatta ttacggagaa agatgcggtc aaattttcag
    10681 acggcatttg caccgataat gtttgggtgt tgcccgtttg tgcgataatc gaacctgatt
    10741 tggcggcgtt tgtgttggag cggttggaag atgtaccgaa ggccgtctga aagcacggtt
    10801 tgggcggagt gattacggat ttgaataaga acgcctcgcg ccgtcattcc cgcgcaggcg
    10861 ggaatctaag tctcgaattt tcaggaatgc ctaggaggct tcagaaatcc caaatctccg
    10921 gatttccact tggacaggaa tgagaaaatc ggtcgtattt tttatctgca ttaatcattc
    10981 gttaaagtat tgaatattaa actgaaaacc ttgttattgc ccttcgccac gctggcattg
    11041 tgcaccaacg cttttgccgc cccgcctagc gacgcgtcgt tggcgcgttg gctggatacg
    11101 cagaattttg accgggatat agaaaaaaat atgattgagg gctttaatgc cggatttaaa
    11161 ccgtatgcgg acaaagccct tgccgaaatg ccggaagcga aaaaagatca ggcggcagaa
    11221 gcctttaacc gttatcgtga gaatgttttg aaagatttga ttacgcccga agtgaaacag
    11281 gctgtccgta atactttatt gaagaatgcc cgtgagatat acacgcaaga agaaattgac
    11341 ggcatgattg ccttttacgg ttcgcctgtc ggtcagtccg tcgttgccaa aaatccgcgc
    11401 ttaatcaaga aatcgatgag tgaaatagca gtatcttgga ctgcattgtc agggaaaatc
    11461 gcgcgacatc atctgcccgc gtttacggaa gagttgcggc gcatcatctg cggcggtaaa
    11521 aatcccgatg cgggctgtaa acaagccgga caggttggga aaaggcatca gaaataaatg
    11581 atagccgtct gaaatattga agagggcatc cgattgattg aaccatcaaa cccgaaagca
    11641 accctatgga aaaaaaattc ttagacatcc tcgtctgccc cgttaccaaa ggcaggctgg
    11701 aatatcatca ggacaaacag gaattgtgga gccgtcaggc gaagcttgcc tatccgatta
    11761 aagacggcat tccctatatg ctggaaaacg aagcgcgtcc gttgggcgaa gaggaactcg
    11821 aagcatgacc gaattcgtcg tattgattcc ggcgcggctg gattcgtcgc gcctgcccgg
    11881 aaaagccttg gcggacatcc acggcaaacc gatggtcgtg cgcgttgccg aacaggcggc
    11941 aaaaagtaaa gctgtgcgcg tcgtcgttgc caccgaccat cccgatattc agacggcctg
    12001 tcaggcgcac ggcgtcgaag tcgtcatgac ttcaaaccgg cacgaaagcg gcacgacgcg
    12061 ccttgccgaa gcctctgccg cgctgaagct gccgccgcat ctggtggttg tcaacgtaca
    12121 gggcgacgag ccgctgattg cacctgaact catcgaccgc accgccgaag tactcgtcga
    12181 aaacaacgtc caaatggcga ccgccgccca cgaattgcac gatttcgacg aattgatgaa
    12241 tcccaatgcc gtcaaagttg tcctcgacaa aaaccgcaac gccatctact tcagccgcgc
    12301 cccgattccc tatccgcgcg atgcgatgcg tgcaggaaaa cgcgaaatgc cgtttgaaac
    12361 cgccgtcctg cgccatatcg gcatctacgc ctaccgcgtc ggcttcctgc aacgctacac
    12421 cgaaatgagc gtttcgccgc tggaaaccat tgaatcgctg gagcaactgc gtgtcctgtg
    12481 gcacggctac ccgattgccg tcgaaaccgc caaagaagct cctgccgccg gcgtagatac
    12541 gcaggaggat ttggacagga ttcgcgctgt atttcagact atataaaata gattcaaagg
    12601 gaaaagatat gcagcaacat attgaaaaat ggcaacactt gagccgggaa gaacagaaaa
    12661 tccttgctga agtatggggt ctcgtgcaaa acgacgatca ggaggttcac tatgaaatgc
    12721 tcaaattgaa cgcacccgac gaagccagtg gcgaattttg gttcagaatg gcagaaacac
    12781 tcagcaccct gccgcccaac cgttccctcg accttagaat gaacggcggc aggctggcga
    12841 ccgccgtatc catcctttcc gtcatgattg aagacaatcc cgacataccg cagctttggg
    12901 cgcaaaaaat taccgcgctc aattatagtg gattaaattt aaaccagtac ggcgttgcct
    12961 cgccttgtcg tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
    13021 tccactatat ttggcacacg gacacaaagc ccgtgccgac ggtttggcac aacagcccga
    13081 caaagcggca gaagccaacg aggaggaata cctgaccaaa gccctgtcgc aaaacctact
    13141 gtcaacattg gatgccgcgc ttgcacgttt tcctgaagac gcgtggtttc aggaaatcaa
    13201 acaggatgca caaaagcatt ttgcttgagg atgtggcagt caggaatatt tccattcagg
    13261 aagaaaagaa gtgcctgatt gggtataatc agggtaaatc ttattttatt tcaaaagatt
    13321 aatatttgct ttctgttttt ccttgacggt atcggaaaag ttgattatag ttacagcttc
    13381 cttaggagta atggctgaga ggctgaaggc acttccctgc taaggaagca tgtggggtca
    13441 acctgcatcg agggttcgaa tccctcttac tccgccaaga tgagaagacg ctgtgtttta
    13501 cagcgtcttt ttttacgcgc tttgttgcgg aagtgccgag ggctgtctgc cgtgcgccgc
    13561 ctggatttgc aatgtgcccg cccgtgcaaa atccgatttg accaacctga agggcaggtt
    13621 tcggaagagc ggggaatttt gataaacaaa aaactgcacg gcataatctt tcccggtttt
    13681 cattttccgt attcgcctat ccgtaccgca aatgctatac tgggaaaaat ttaccgattg
    13741 tgttttacgg catatttgcc gataggatgg aagagacaaa tgagcagaat ccggcaggct
    13801 tttgccgctt taaacggtga aaaggcattg attccttata ttacggttgg agacccggac
    13861 attggggcga ctttggcaat aatgcacagt atggtggaaa acggtgcgga tattttggag
    13921 ttgggcgtgc cgttttccga tccgatggcg gatggaccgg ttattcagcg tgcggcggag
    13981 cgggcgttgg caaacgggat ttcgctgcgc gatgtcttgg atgtcgtcag aaaattccgt
    14041 gaaaccgaca cgcaaacgcc ggttgttttg atgggatatt tgaaccctgt acataagatg
    14101 ggttatcggg agtttgctca ggaagccgca atggcgggtg tggacggcgt gttgacggtg
    14161 gattcccctg tcgaaaccat cgatccgctc tatcgcgagc tgaaggataa cggggtcgac
    14221 tgtattttcc tgattgcgcc gacgacgacg gaagaccgta ttaaaaccat tgccgagctg
    14281 gcaggcggat ttgtctatta tgtttcgctc aagggcgtaa cgggcgcggc aagtttggat
    14341 acggatgagg tttcgcgtaa aatagagtat ttgcatcagt atatcgatat tcccatcggt
    14401 gtcggtttcg gcatcagcaa tgcggaaagc gcgcgcaaaa tcggccgggt tgccgacgcg
    14461 gtcattgtcg gcagccggat tgtgaaagag attgaaaaca atgcgggcaa cgaggctgcg
    14521 gtcgtcggtg ctttggtcaa agagttgaag gatgccgtgc gctgattctg tcacgcattc
    14581 taaatatttt aggagttgtc catgagctgg ttagataaaa tcctgccacc caaaatcaaa
    14641 aaccgcagca aagacggttc ttccaatgtt cccgagggtt tgtggcacaa atgtccgtct
    14701 tgttcggcaa ccgtttattc gaccgagttg cagcagaaca atcaggtttg tccgaaatgc
    14761 aaccaccaca atccgttgtc ggcacgacaa cgtctgaacc tgcttttgga tgaggatggc
    14821 agggaggaag ttgccggaaa tgtcaaaccg acagatcctt tgaagtttaa agacggcaaa
    14881 aaatatccgg atcgtttgag tgcggcacgc aagctgaccg gggaagatga tgctttggtg
    14941 gtgatgaaag gcaagatgaa cggcctgccc gtcgtcgttg ctgcgtttga gttccgcttt
    15001 atcggcggtt cgatgggttc ggttgtgggc gaacgattcg tacaaggtat ccgtcgggcg
    15061 gttgccgaca attgtccgtt cgtctgtgtg gcggcttccg gcggcgcgcg tatgcaggag
    15121 ggtgtaaact cgctgatgca gatgacgaaa accagtgccg cgctgcattt gctgacggaa
    15181 aaacgcctgc catttatatc ggtgttgacc gatccgacta tgggcggcgt atccgccagc
    15241 ttcgcatttt tgggcgatgt cgtgcttgcc gaaccgaacg cgctgatcgg ttttgccggt
    15301 ccgcgcgtga ttgagcagac ggtgcgcgaa acgctgccgg aaggcttcca acgcgccgag
    15361 ttcctgctgg aaaaaggcgc aatcgaccag attgtcgacc gccgcgatat gaagcggcgc
    15421 atcagtgatt tgattacgct gttgtgccgt caggacaaag tttccgccgc ctgatggctg
    15481 atgaatcgag taccgtctga aaccgatgtt tcagacggta tttttgtgtc tggttatttg
    15541 ttgtgcggct ttatcgatgg ggcatagcgt ccggcacgtt ctttcaggcg ttgtaccaaa
    15601 cctttcgtgt cggcgggtac accgccctcg cagaatgcct gatacaggac ggtgcgcagt
    15661 gcgtcgttgc ggcttaatgt accgcctatc ggtttccatt cggcgtttcg gggctgtatc
    15721 cagcggcggt tgaccgtgtc gccgtagccg aacactttat aggaggaagg tttgccgttg
    15781 ccgaacctga tggagaagcg ggcgcagaat atgccttcgg cagtcaggtt gtcgtagcct
    15841 ttgtcggagc ggatattgag aatgtagcgg atgctgccgt cgggcgcggg cataatttgc
    15901 aggctgtcga gcaggatttt cggctgtttg ccgtaatttt catccacata aatgtcgaac
    15961 cagccgtccg agtgcgtatc gggcagcggc ggcagtttgg cggtatgttc tttaaattcg
    16021 cgggcggcgg cttcttcggg cgtttcgcgg tagcgggtgt tgatcggcgt gtctttttgg
    16081 ctgaagccgg cagcaaggga cgtgccggcg gtgagtgcga gcagcaggag ggcggtgcgg
    16141 cgcataagtt tctccaaatt gaaaacggcg ttattttatg ggttggcaaa gggggctgca
    16201 agcaagtggg gtataattca gctttattta aacttttaac cggtatcgga acatgaacag
    16261 cgaaactttg gacgttatcg ggctgaaatg ccctttgcct attttgcgtg ccaaaaaggc
    16321 tttggcgcaa atgcagcagg gcgacgtgtt gaccgttctg gcaaccgacg gcggcgcgcc
    16381 gggggacttt gaggcgttct gccgccaaac cggtcatgtg ctgttggatt cttccgaaca
    16441 ggacggcgtg ttcacgctgg tcgtcaagca caaataaatg ccgtccgaaa tgcggatgtc
    16501 ccgattgatg ttattgctgt cggcatttgt gcttgctgcg tgtaatgccc aaaaccggca
    16561 atccggtgta cggcatcagg atttcgcgcg tgtagccgtc gatttgtttc ggagtgcctg
    16621 cgtcctgact caaggcagtt ttgaatcggt ttccaagttt gccgctgtcg gacattttgt
    16681 ccctgtcgga aaggaagacc tgtcccatct gccgcccact gttgcagaac ccgatgtaca
    16741 ggcgttgtgg acgcttgaac gcgatggcgg catttattat ttgagcctga cgcgcgacag
    16801 ttgcagtgtg aaagccgagc gggcagacag tgccgcgctg ttggaacatt ttgacggact
    16861 tgtccgacag ccgccgaaaa atgccaatat agaattgagg gcggaacagt cggtgcaggc
    16921 atcatttgaa atccgccagg tcagctatgc ctggcgggaa gcgggcagct ccgaagaaac
    16981 cgtattgacc gcacaaaccg ccgattcccc ggatttaccc gtacaggcag ttttgaacct
    17041 gacgcaccgt tcccacaacg ggaaacccct aattctcccc taacttcaga cggcataaag
    17101 tcggcatgct gtctggaaac caaaaatcta aaaaggaaca accatgcaaa ccctgaccat
    17161 catccgcccc gacgatatgc acctgcacct gcgcgacggc gacgcgctca aagccgttgc
    17221 accctatacc gcccgccaga tggggcgcgc cgtcatcatg cccaacctca aaccgcccgt
    17281 cgtcagcgta gccgacgcgc ttgcctacaa agcgtgcatc atggcggcgt tgcccgaagg
    17341 cagcgcgttt gagccgttga tgacgcttta tttgactgat aacgccacgc ccgaacttgt
    17401 acgcgaagcc aaagccgccg gcatcgtcgc cttcaaactc taccccgcag gcgcgacgac
    17461 caattccgat tccggcgtaa ccgacctgtt caaactcatc cccgttttgg aagaaatggc
    17521 gaaacaggac atcctgttcc tcgttcacgg cgaagtaacc gaccccgaaa tcgacatctt
    17581 cgaccgcgaa gccgccttta tcgggcgcgt gatgaaaccc gttttggcgc aagtgccgaa
    17641 tcttaaagtc gtgttcgaac acatcaccac cgccgaagcc gcccgcctgg ttttggaagc
    17701 aggcgacaac gttgccgcct ccgttacccc gcaacacctc ctgctcaacc gcaacgacct
    17761 cttggtcggc ggcgtgcgcc cccatcattt ctgcctgccc gtcctcaagc gcgaaaccca
    17821 ccgtcaggca ttggttgccg ccgttaccgg cgagaaggcg cacaaattct tcctcggcac
    17881 agattccgcg ccgcacgcca agtccgccaa agaaaatgtc tgcggctgcg ccggtatgtt
    17941 cagcgcgatg accgctgtcg agctttacgc cgaagtgttt gaaaaagcag gcgcgttgga
    18001 caaactcgaa gcctttgcct cgaaaaacgg cgcaaggttc tacggcattc ccgaaaatac
    18061 tgacacgatc accctcgtca aacaaagcca aaccgtcccc gcaagcgtcc cctacggcga
    18121 cggcgaactt gtcccgatgc gcgcgggcgg cgaaatcggc tggacggtac agtattgatg
    18181 gggctggaaa caaaatgccg tctgaaaggt ttttcagacg gcatttgtgt tgttgactga
    18241 ttgatacgtc aacggcgttt gagtttgtta cgtttcggtt atttccgata aattcccaca
    18301 attttcaaat ttcgccattc ccacgaaggc aggaatccgg aaattcgatg cgaccagagt
    18361 ttatcaaaaa cggcagcaac tcaaaaaacc agattcccgc ctgcgcggga atgacgagat
    18421 tgaagtttca gaatttattt gaaataccca aaattcaaaa aaccaaattc ccacctgcgt
    18481 gggaatgacg aaacaaagaa agcagaaata aggacataga actttcttta aatttgtgat
    18541 gcatcaacgg cgtttgggct cgtcgggacg gatttgggcg gcgagtttgt cgaggatgcc
    18601 gttgacgaat ttgtgcccgt ccgtgccgcc gaaggttttg gttacttcga tggcttcgtt
    18661 gatgatgacg gggtagggcg tttcgggcat ggcggacagc tcgtggcagg cggtcagcag
    18721 gacggcgcgt tcgatggggt tgaggtcttt ttcgtcgcgg tcgagcaggg ggcggatttg
    18781 tcggatgtac tctgccgcat tggtttgcgt gccgaagaaa agtttgttga acaattcttc
    18841 gtctgccttg gcaaagtcgg gcatttcgcg gatgttttta gcaatctcgg gcgcggcggt
    18901 gcggttgata agggattggt aaacggcttg tacggcaagc tcgcgggaac ggcggcgggc
    18961 tgttttcatg atttttcctt gaaacggttg ggcggcacgg tatgccgtct gaaacggaaa
    19021 gggcgtatcg gtgtacgccc tgtttgttat tcttcgtctt caaactgttc ttcgagcagg
    19081 aggttgacga ggttggcgca ttctacggcg actttggcgg catccgaggc tttttcttca
    19141 atccgttcga ttgcctgtgc gtcgttttcc gtggtcagga cggcattggc aatcgggatg
    19201 ttgtagtcga gtgcgacgcg gctgacccct gctccggatt cgttggaaac cagctcgaaa
    19261 tggtaggttt cgccacggat aacgacgccg atggcaatca gtgcgtcaaa tttttcagaa
    19321 gaggcaaagt tcatcagcgc gatggggatt tcaagcgcgc cgggtacggt ggcgacggta
    19381 atgttttcgt ctgccacgcc caattcttgg agggtgcggc agcagacttt gagcatttcg
    19441 ctgccgattt cgttggtgaa gcgtgcctgt acgatgccga tgcggaggtg tttgccgtcg
    19501 aggttggggg cgatggtgtt cattgggtgt cctttggtat tcggaggttt cggaatgccg
    19561 tctgaaggtt ttagtcttgc ggctgccagt cggcgacggt ttggaatgtg ccgtcttctg
    19621 ccaattccca cgcgctgcct tcaggttggg agagcagtgc ggcggtttca gggttggttt
    19681 tggtgatatc ggcgaggctg acgatgctga agttgtccgg gtcgtcggtg tattcgtcgg
    19741 tctcgtcgcc gctgaagaaa cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt
    19801 agaggaagcc gacgggctgg ttttgtttgg cgacggtgtt ggtggcgatg cagcggtcga
    19861 gtgccgagga aagtgcttgt gcaaatgcgt tcattgcggg aatacgttgg gggaaaactt
    19921 gcggatttta ccacgattct tgcgttgtcg gcagacggcg gcggtttggt ggtacaatgt
    19981 gcgccgtttg cagccttaag gtgtttctgt atttttggag tatggaaacg cattcgggct
    20041 gttttttgcg gaagacggta atgaaagacg atgttttgaa acagcaggca cacgcggcga
    20101 tacagaagag actgggctac gcgttccgcg atatttcgct tttgcggcag gctttgacgc
    20161 acaggagcca tcatgcgaag cacaacgagc ggttcgagtt tgtcggtgat tcgattttga
    20221 attatacggt ggcgcggatg ctgtttgaca cgtttccgaa gttgaccgag ggcgagttgt
    20281 cgcggttgcg ggcaagtctg gtcaatgagg gcgtgctggc ggaaatggcg gcggaaatga
    20341 atgtcggcga cggtctgtac ttgggggcgg gcgagttgaa gagcggcggc ttcagacggc
    20401 cttcgatact ggcagacgcg atggaggcga tgtttgcggc ggtcagcttc gatgccgatt
    20461 tcaacacggc ggaaaaggtg gtgcgccatt tgtttgccga ccgcgtccgg tgtgccgatt
    20521 tccaaaatca ggcaaaagac ggcaaaactg ctttgcagga ggcgttgcag gcacgccgct
    20581 ttgctttgcc gaaataccgt atcgaagagc aaatcggcca tgccaacgac agtatgtttg
    20641 tggtttcctg cgatttgggc gaactgggtt tcgtgtgccg tgccaaaggg acgagccgca
    20701 aggcggcgga acaggaggcg gcgaaagagg ctttgaaatg gctggaagag aagctgccgc
    20761 tgaagaagaa aaagaaatga ggcggcgcgt gaatatgccg tctgaacata tggatacgaa
    20821 agcaaatatg gatattgaaa ccttccttgc aggggaacgc gccgccggcg gataccgttg
    20881 cggcttcgtg gcgattgtcg gccgtccgaa cgtgggcaaa tcaacgctga tgaaccatct
    20941 catcggtcag aagatcagca tcaccagcaa aaaggcgcag acgacgcgca accgcgtaac
    21001 ggggatttat accgacgata ccgcgcagtt cgtgtttgtc gatacgcccg gctttcaaac
    21061 cgaccaccgc aacgcgctca acgacaggct gaatcaaaat gttaccgagg cgctcggcgg
    21121 cgtggatgtg gtggttttcg tcgtggaggc gatgcgcttt accgatgccg accgcgtcgt
    21181 gttgaaacaa ctgcccaagc acacgccggt cattttagtg gtcaacaaaa tcgacaagga
    21241 caaggcgaaa gaccgttacg cgctggaggc gtttgttgcc caagtgcgcg ccgaatttga
    21301 atttgcggcg gcggaggcgg tcagcgcgaa acacggattg cggattgcca acctgttgga
    21361 gctgattaag ccgtatctgc ccgaaagcgt gccgatgtat cccgaagata tggttacgga
    21421 caaatcggcg cgttttttag cgatggaaat cgtgcgtgaa aaactcttcc gctatttggg
    21481 cgaggaattg ccttatgcga tgaatgtcga agtggagcag tttgaagagg aagacggttt
    21541 gaaccgcatc tacatcgccg ttttggtcga taaggaaagc caaaaggcga ttttaatcgg
    21601 caaaggcggg gagcgtttga agaagatttc caccgaagcg cggttggata tggaaaaact
    21661 gtttgatacc aaagtatttt tgaaggtctg ggtcaaagtc aaatccggtt gggcggacga
    21721 catccgcttc ctgcgcgagc tgggtttgta gtttttcttg ctgaacttta cgcaaatgcc
    21781 gtccgaacag gtttcagacg gcattttgtt tcaatcggga atatctttgt taaaaacggg
    21841 ttgatattat ctgtgcatat tatagtggat taacaaaaac cagtacggcg ttggctcgcc
    21901 ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg attccgtact
    21961 atctgtactg tctgcggctt tgccgccttg tcctgatttt tgttaatcca ctataaagac
    22021 cgtcgggcat ctgcagccgt cattcccgcg caggcgggaa tctagtccgt tcggtttcgg
    22081 tttttttggc tagtgccgca acattaaatt tctagattcc cactttcgtg ggaatgacgc
    22141 gattagagtt tcaaaattta ttctaaatag ctgaaactca acgcattgga ttcccgcctg
    22201 cgcgggaatg acgaatttta ggtttctgtt tttggttttc tgttttttcc gataaatgcc
    22261 gcaacctcaa atcccgtcat tcccgcgcag gcgggaatct agacattcaa tgctaaggca
    22321 atttattgaa aat
//
LOCUS       DAWHXS010000025        21812 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWHXS010000025 DAWHXS010000000
VERSION     DAWHXS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.025"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            127..678
                     /locus_tag="WIE22_000720"
     CDS             127..678
                     /locus_tag="WIE22_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126848.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(955..1647)
                     /locus_tag="WIE22_000721"
     CDS             complement(955..1647)
                     /locus_tag="WIE22_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ4126849.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1953..2510)
                     /locus_tag="WIE22_000722"
     CDS             complement(1953..2510)
                     /locus_tag="WIE22_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126850.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(2755..2913)
                     /locus_tag="WIE22_000723"
     CDS             complement(2755..2913)
                     /locus_tag="WIE22_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126851.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2910..3752)
                     /locus_tag="WIE22_000724"
     CDS             complement(2910..3752)
                     /locus_tag="WIE22_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4126852.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3990..4910
                     /gene="pyrB"
                     /locus_tag="WIE22_000725"
     CDS             3990..4910
                     /gene="pyrB"
                     /locus_tag="WIE22_000725"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ4126853.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4920..5378
                     /locus_tag="WIE22_000726"
     CDS             4920..5378
                     /locus_tag="WIE22_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ4126854.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5443..6108
                     /locus_tag="WIE22_000727"
     CDS             5443..6108
                     /locus_tag="WIE22_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126855.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6171..7388)
                     /locus_tag="WIE22_000728"
     CDS             complement(6171..7388)
                     /locus_tag="WIE22_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ4126856.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7530..8075
                     /gene="def"
                     /locus_tag="WIE22_000729"
     CDS             7530..8075
                     /gene="def"
                     /locus_tag="WIE22_000729"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ4126857.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8162..9088
                     /locus_tag="WIE22_000730"
     CDS             8162..9088
                     /locus_tag="WIE22_000730"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ4126858.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDAGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9167..10426
                     /gene="rsmB"
                     /locus_tag="WIE22_000731"
     CDS             9167..10426
                     /gene="rsmB"
                     /locus_tag="WIE22_000731"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ4126859.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLASSCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10392..10991
                     /locus_tag="WIE22_000732"
     CDS             10392..10991
                     /locus_tag="WIE22_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ4126860.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10991..13111
                     /locus_tag="WIE22_000733"
     CDS             10991..13111
                     /locus_tag="WIE22_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ4126861.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQQAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            13104..14381
                     /locus_tag="WIE22_000734"
     CDS             13104..14381
                     /locus_tag="WIE22_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ4126862.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14472..15659
                     /gene="dprA"
                     /locus_tag="WIE22_000735"
     CDS             14472..15659
                     /gene="dprA"
                     /locus_tag="WIE22_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ4126863.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGEKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15685..16146
                     /locus_tag="WIE22_000736"
     CDS             15685..16146
                     /locus_tag="WIE22_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ4126864.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16225..18531
                     /gene="topA"
                     /locus_tag="WIE22_000737"
     CDS             16225..18531
                     /gene="topA"
                     /locus_tag="WIE22_000737"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ4126865.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18604..19299)
                     /locus_tag="WIE22_000738"
     CDS             complement(18604..19299)
                     /locus_tag="WIE22_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126866.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            complement(19319..19870)
                     /locus_tag="WIE22_000739"
     CDS             complement(19319..19870)
                     /locus_tag="WIE22_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126867.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHFHIQNGAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19989..20366
                     /locus_tag="WIE22_000740"
     CDS             19989..20366
                     /locus_tag="WIE22_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4126868.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            20455..21024
                     /gene="rsmD"
                     /locus_tag="WIE22_000741"
     CDS             20455..21024
                     /gene="rsmD"
                     /locus_tag="WIE22_000741"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ4126869.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21147..21398
                     /locus_tag="WIE22_000742"
     CDS             21147..21398
                     /locus_tag="WIE22_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ4126870.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            21495..21578
                     /locus_tag="WIE22_000743"
     tRNA            21495..21578
                     /locus_tag="WIE22_000743"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21529..21531,aa:Tyr,seq:gta)
     gene            21609..21682
                     /locus_tag="WIE22_000744"
     tRNA            21609..21682
                     /locus_tag="WIE22_000744"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21641..21643,aa:Gly,seq:tcc)
     gene            21692..21766
                     /locus_tag="WIE22_000745"
     tRNA            21692..21766
                     /locus_tag="WIE22_000745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21724..21726,aa:Thr,seq:ggt)
BASE COUNT         5370 a         5484 c         6028 g         4930 t
ORIGIN      
        1 gacgttttaa caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc
       61 aagtttgtcc tagttatcta ggacagcccc ttattttttg ttcggcggct tgcgtggtcg
      121 ggtaaaatga aggttttgaa cggttggtcg gacaggaaga tgtggcgggt tttgagtgct
      181 ttgccgatag gcgtggtgtt ttttgatttg atctacggtt ttgtgttgaa tgtgttgcag
      241 ggtttggatt tgcagcgtgc cgtgccggat ttggaaggcg tgttggcggt tacgcccgat
      301 attgcattca acagtttgca gattgtcgcc aacggcggta tggcggcggt ggtctgtttc
      361 gggttggcgg ttgtgttttt gctcaaccgt tcggtgcggc ggcggcaggt gttggaaatc
      421 ggggtgttcc ggatgttggg gctggtggcg gtattggcgt tcagcgcgcc gtcggtgtgg
      481 gagtgggcga acgcgctacc gctgctgctg aagggcgcgg acgtggtcaa tacggggaat
      541 gcgcgttatg tgctgacggc tttgtgtatg ccctttccgg cggtgtcgtg cgtcatcggg
      601 ctggtggggc ggttcaggct tcagacggca tcgggcaggg cggcaaagtc agggggtgcg
      661 ggcaaggcgg acggatagga cgcatttttc agcgggtgcg tcgagaagca gccgatgtgt
      721 ttggcagccg cagcttgggg gggtgtagtg ctaatggcgg tttctttgct tttatagtgg
      781 attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg
      841 tgctgaagca ccaagtgaat cggttccgta ctattggtac tgtctgcggc ttcgtcgcct
      901 tgtcctgatt tttgttaatc cactatataa aataaatggg caaaaatcgg tttattatcg
      961 tttttgccgc atttggattt gttctaccgt aaaacgtgtt tgacgaacgg gattcttatt
     1021 aaaaaacatc tgatttctaa caaaatcagt attttttggc acgatggcta aaatttttcc
     1081 ttccatttcg ccatcacgtg ttttccatgc gctcaagaat tgtgatttgc tcattgagac
     1141 gtgccccagc gatggatcag ccagcaaaac agtttctccg ttaataccgt tcaataccga
     1201 aaaatggttg tttttacggt attttaaata cacaattaca ggaattttta gttgtaccaa
     1261 ctgttcaaat ggcaaagcat aaccttgtgc ttcaaaaccc agttcgggca ttatgcgttg
     1321 catatcatca aaagaagcac gcatttgggt tttatccatt ttgtccaaga tttccgcttc
     1381 agaataatgt ctgccataaa aattattcag taacgtggca atcgaagccg cgccgcaaga
     1441 aaaatccaaa tcttgtttta ctatgccgga atctcgccgt gctttccaac tccgtacatg
     1501 gatgttttgg taagaagcgg ggttttcagc aaatataggg taagcaaaaa caatatttgg
     1561 gataaagcca attaaaaccg catggtttat taatttagaa atatttttta tcatagtata
     1621 tatgcgtaga ataaacgcat gaaacataaa aaaataaaat catatgcaat tttattgcat
     1681 gaataaatat gaataaaata gataatgatg ggagtaaata cgccatgtat tttggaagtt
     1741 taaatttatt aataataaaa taatttatcg tagcgcaaat aaatcccaaa attggaacga
     1801 ttagaaaaaa aattaataca cccatcatca tgcaaactct atattaataa atagctaact
     1861 aatgctattg tggaaattag ttagctaact aaaagttatt aatgattatt tccgagaatt
     1921 gactgcattg ttggcagcat tggccccaaa acctagtgca tgaatacccg gtctccatgc
     1981 caaatttcca gccaatccgc ctccggcagc agcagccagc cctgttttgc cgctactcct
     2041 gttgccgcac cgattcctgc cgcagtagcc gcgccttgcg cagttcctaa tttaccatga
     2101 ttatacaaat tagcaccatg atactcccat gcacctaatg caccgccaaa agcagcggct
     2161 gcaataatgg gaacaaattc accttgtgtt tctttcattt cagcctgtga taattgaatt
     2221 gctttcacat tttggctgtc aaaaacttgg ctgtctaaat tttgcgccat tacaggtgta
     2281 atcatcatag ccattacagt tgcaattttc gttgcgctgg tttgcacata aataggatta
     2341 gcaaattcgc tttgattgcg ttcagtgttg atgtagctaa tactgctttc tagtttgaat
     2401 ttacccttgt cagtcaataa ttcttccaaa cttaaaggta agtcagcata agcaatttgg
     2461 ctggcaacag tcaaaataaa atctattaga catttgtgtt tttgcatcat ttcgtttgat
     2521 tttctagttt ttgagaatga tacaaagttt ttttacaaag taaagagtca ctctgaaaaa
     2581 cattttttca ttataaatca aaatattgat agaataaata gcgagcatcg attcacggtg
     2641 tactttagtg caaagcgtac aacgcgagcc tgaaccacca gccgcaacag gaaaagaaag
     2701 ccgatagagt gcaatgcttg ccaacgtgca agcgagcttg caagaacgct tggctcaacg
     2761 agagcaggca agacagaaag cagaaagcag gataggagcg gtaacgcaaa ggtctcgggc
     2821 tttgatttcg ccgtaaaccc tgctgccgcc ttgtccggaa agggtgcagg cggcgagtgc
     2881 cgacagggtg cagatgggga gggggggttt catttggggt cgcaacggaa gtggtatgcg
     2941 cagatttcaa aaccgttttt gaaatacagg cggtgcgcgt cggcacggtc gtggttgacg
     3001 tggacgttga ggtggatttt ggttacccct gtttccgcgc cgattttgcg gacttcttcc
     3061 aaaaggcgcg aggcgtagcc tttgcggcgg ctttgcggca gggtaacgat gtcatcgatg
     3121 tggatgtggc ggccgctggc gagggtgcag gcttcgcgga agccgcagac ggcgacggca
     3181 ttgtgtttgc cttcttcaaa aatacccagc aggcggtagc cttgggggcg ttggactttg
     3241 ttgatctgtt cggtaaagcg gttgatgtcg gtcagggcgg aacgcaaaac gctcaaggct
     3301 gcaaaggcgg tggcggtgtc gtccgcgccg atttcgcgca aaacgtagga tgcgcccgag
     3361 gcggtctgtt cctgtgcttt ctcggcggcg tgtttttctt cgattgcctg tgccagcatg
     3421 acgtgttcgt cggcagggtt gttttgtccg ccctgttcgc gttcttcgag cagggctttg
     3481 cagtcgatga cgcgcaggtc gttgtcggcg gcaaagtcca tcaggaagcg gaacatttgg
     3541 gggttgtcgg tttccagttt tttatcgacg gcgacgcagc ggatgttgtc caccagcatg
     3601 gggcgcaccc atttcgaata ggaaagcctg tggtctttgg tgaacgagga cagaatgccg
     3661 cacaggcgtt ccgcccagtc gctgggacgg aaaatcttgc cggaactcgt tgtgccgtgg
     3721 atgacgactt cgtaggggtt gcagactaac atggcggctt cctgaaaaga aatgtctggc
     3781 gcgattatac cttatgctta tgcgggcgtg tttggatatg ccgtctgaaa agtacgggat
     3841 tcgtgcggta aaactttgcg gcggcaaatg tgcgataata cgcgccgtat tgccgctttt
     3901 gcgaagctgt tccgcaaaca tacgggcggc gtggacgacg tataaccgga tacccgcctg
     3961 acgcgggttt tttacggaag ggggcaaaaa tgcctaatcc gctttacaga cagcacatca
     4021 tctcgatttc ggatttgtcg cgcgaacagt tggaatgcct gcttcagacg gcattgaagc
     4081 tgaaggcgca tccgcgcggc gatttgttgg aaggcaaact tatcggttcg tgcttctttg
     4141 aaccgtccac gcgcacgagg ctgtcgtttg aaacggcggt gcagcggctg ggcggcaagg
     4201 tcatcggctt ttcggacggc gcgaatacca gtgccaaaaa aggcgagacg cttgccgata
     4261 ccgcccgcat catttccggc tacaccgacg caatcatcca acgccacccc aaagacggcg
     4321 cggcgcgcgt ggcagcggag ttttcgcgcg tccccgtcat caacgccggc gacggcacca
     4381 accagcaccc cagccagacc ttgttggatc tggtaacgat ttatgaaacg caggggcgtt
     4441 tggacaagct caaaatcgcc atggcgggcg acttgaaata cgggcgcacc gtgcattcgc
     4501 tttgtcaggc gttgaaacgc tggggctgcg aatttgcctt tgtttcgccg cccagcctcg
     4561 cgatgcccga ctacattacc gaagagttgg acgaagccgg ctgccgatac cgtatcctcg
     4621 gcagtttgga agaggcggcg gaatgggcgg acatcctgta tatgacccgc gtccagcgcg
     4681 aacgtttcga tgaacaggaa tttgccaaaa tccaaggcaa attcaatctc gacgcatcca
     4741 tgcttgcccg cgccaaaccg aacctgcgcg tgctgcaccc tttgccgcgc gtggacgaaa
     4801 tccatcccga tgtcgatgct acgccgcatg cctattattt cgagcaggcg accaacggcg
     4861 tttatgcgcg tatggcgata ttgtcgctgg tgttgaacga agaagtgtga ggaaccgata
     4921 tggaaacccc gaaactcagt gtcgaagcca ttgaaaaagg tacggttatc gaccatattc
     4981 ccgccggcag ggggctgacc atcctgcgcc agttcaaact tttgcactac ggcaacgcgg
     5041 taaccgtggg cttcaacctg cccagcaaaa cccaaggcag caaagacatc atcaaaatca
     5101 aaggcgtgtg cttggacgac aaagccgccg accgcctcgc cctgttcgcc cccgaagcgg
     5161 tggtcaatac catcgacaat ttcaaggtcg tgcagaagcg gcatttgaac ctgcccgatg
     5221 aaattgccga agtgttccgt tgcccgaaca cgaattgcgc cggccacggc gagccggtca
     5281 aaagccggtt ttatgttaaa aaacacaacg ggcagacgcg gctgaaatgc cattactgcg
     5341 aaaaaaccta cagccgggat tcggtggcgg aagcctgacg gattccctta aaccgagcgg
     5401 gcggcatttc gtctgccgct tgttttgcca atctgaaatg gaatgatgat gcacgcttct
     5461 gtccaaagcc gtttcgcacc gatactttat gttttgattt tctttgccgg ttttttgacc
     5521 gcgcaaatct ggttcaatca gaaagcctat actgaagagc tgcctccgct tctgtccgca
     5581 ttgtccgccg tcgcgctggt gtggctggcg tgggcgttcg tgtcggcgcg ttcaaaggct
     5641 aaggcggaaa agttctaccg cgaaaaaatg atacagaacg aaagcataca tcccgtcctg
     5701 cacgcttctt tgcaacactt ggaacacaag ccgcaaatgc tcgccctgct ggtcaaaaac
     5761 cacggcaaag ggatggcgga acaggtcagg ttcaaggcgg aagtgctgcc cgacgacgaa
     5821 gacgcgcgca cgattgccgc cgagttggca aaaatggata tgtttgcatt ggggacggac
     5881 gcggtcgcct cgggcgaaac ctatggacgc gtgttcgccg atattttcga gttgtcggcg
     5941 gctttggaag ggcgcgcgtt caaaggaatg ttgaaactga cggcggaata taaaaacatc
     6001 ttcggcgatg cctgccgttc ggaaacggcg ttggagttgg gcgcgctcaa tcaggcgttg
     6061 caggagattt caaaaacatc ggaaaagtcc aaacggatat tttattgaag atggaaaaat
     6121 gccgtctgaa acggaaggtg tttcagacgg catttttgtc ggatgattaa ttattcggag
     6181 cggttgaagc caaacttcac gcggctgcgg ccctgatccg gtatattgtc caaatcgcgt
     6241 cccggattgg cggcggtgtc gcctacggaa atatcggaga tgttttccaa aatgatggcg
     6301 gacgacaggt gttcggaggt gcggtaaacc attgccaagc ccacttcttc ggcaggagtg
     6361 gaaatcagct cgacggtatc cctgcttttg aaattgttgg agaggtcgac ctgcatcgtt
     6421 ttcttgcgtt tgtagaggct caaaaccgtg cctttgtcca aaccgtccgc ctcgcctttg
     6481 tcgatggtga tggtttgaaa ctggccggca atccttgtgc cttcaaacac ggaaacgatt
     6541 ttagcctgaa ccgggcggga cggttcgtgc ggcatcatgt tgaagcggtc ggtgtcttcc
     6601 ggcattttca tcaggtagtc gccctgctgt atttcggaaa tggcggtttc gaccaccagc
     6661 ggctgtatcg aaggggtgcg cagcggggta atcaaaggat gggtgcgggt atggtattcg
     6721 ttgtctttcg gccgttctcc agcctgtttc gagcgttgtt cgaggacaga gtcggtatag
     6781 tcgagggagc gcacgatgcc gctgaatgcg acttcctgcc cgaggaattt acccgtatcc
     6841 ggatcggtga tgtttttatt gattcggtag gtcaggtagc ggcccggctc tttcaggcct
     6901 ttggtgtaaa ccctggtgcc tttggtgtac agcagcctgc cttccgggcc cgagagcagg
     6961 cgcggcgcgg cagcggtttc tttgcgggaa acgatttgcg ggtgccgcat aaagatgcgg
     7021 tagaagttga catcgatggc gggaataccg tatccggaca cttccttatc cggactcatt
     7081 ttgacgacgg ggatgccgtc tgtctgttcc aagccgaggc gcggttcgcc gtcaacgtgg
     7141 cgcaacacca atacctggtc cggataaatc aggtcgggat tgtggatttg atcccggttc
     7201 gcgtcccaca ggcggcccca ttgccacggg ctgtacaggt atttgcccga aatgccccac
     7261 agggtgtcgc cctgtttgac cgtgtagcgt tccggcgcgt tcgggcgcac ctccaaattt
     7321 gccgccaaag tttgtgttga gaatgccata cctgccgcgc agagcagggt tataatacga
     7381 cgttgcataa ccgttcccct tatctgataa atttcggttt gtcttgcttg attgggttgg
     7441 aaaaagcggc ggcagcccct cgggatgtgc cgcgtgataa aaaatgttcc gcattttaac
     7501 atcgaattat ccgcaccatc acggtaatta tgaaaaacag gcggcgtatc cgccgaagga
     7561 aagagaaaat tatggcttta ttgaatatct tgcaatatcc cgacgagcgt ctgcacacgg
     7621 tggcaaagcc tgtcgaacaa gtcgacgagc gcatccggaa gctgattgcc gatatgtttg
     7681 aaacgatgta cgaatcgcgc ggcatcgggc tggcggcgac gcaggtcgat gtgcacgagc
     7741 gcgtggtcgt gatggatttg accgaagacc gcagcgaacc gcgcgtgttc atcaaccccg
     7801 tcatcgttga aaaagacggc gaaaccactt acgaagaggg ctgcctgtcc gtgccgggca
     7861 tttacgacac cgtaacccgc gccgaacgcg tcaaggtcga ggctttgaac gaaaaaggcg
     7921 aaaagttcac gctggaggcg gacggcttgc tggcgatttg cgtgcagcac gagttggacc
     7981 acctgatggg tatcgtgttt gtcgaacgcc tttcccaact caagcagggg cggattaaga
     8041 ccaagctgaa aaaacgtcag aaacatacga tttgaccctt ttgccgtgcc gtctgaacgc
     8101 tgcaaagttt tcagacggca cggtcttgtc cgacaatttt acgcacacgc aggaacacgc
     8161 tatgaaagtc atcttcgccg gcacgcccga ttttgccgcc gccgccttaa gagccgttgc
     8221 cgccgccggt tttgaaatcc cgctggtgct gacccagccc gaccgtccga aagggcgcgg
     8281 tatgcaactg actgccccgc ccgtcaaaca agccgcgctg gaactcggtt tgcgcgtcga
     8341 acagcccgaa aaactgcgta acaacgccga agccctgcaa atgctcaaag aggtcgaggc
     8401 agacgtaatg gtggttgccg cctacggttt gattctgccg caagacgtgt tggatgtgcc
     8461 gaaacacggc tgcctcaata tccacgcttc gctgctgccc cgttggcgcg gcgcggcgcc
     8521 gattcagcgc gcgattgagg ccggcgatgc cgaaaccggc gtgtgcatta tgcagatgga
     8581 tgccggtttg gacaccggtg atgtggtcag cgaacaccgc tacgccatcc aaccgaccga
     8641 taccgccaac gaagtccacg acgcgctgat ggaaatcggt gcggcggcgg ttgttgccga
     8701 tttgcaacag cttcaaagca aaggccgtct gaacgcggtc aaacagcccg aagaaggtgt
     8761 tacttacgcg caaaaattgc gcaaagaaga ggcgcgtatc gattggagcg aaagcgcggc
     8821 ggttatcgaa cgcaaaatcc gcgctttcaa ccccgtgcct gccgcgtggg ttgagtatca
     8881 gggcaagccg atgaaaatcc ggcgggcgga agtggtggcg caacaaggcg cggcaggcga
     8941 agtgttgtcc tgttcggcgg acggtttggt cgttgcctgc ggcgaaaacg cgctgaagat
     9001 taccgaattg cagcctgccg gcggcaggcg tatgaatatc gcggcgtttg cagcaggacg
     9061 gcatatcgaa gcagggacga agctgtaaat cccttcagac ggcattccga tccgcaaacg
     9121 ggaatgccgc ctgaaaccat cagttgaaga aagcgaatca cataatatga gtatggcact
     9181 tgcccaaaaa cttgccgccg acagcattgc ggcggttgcc gaaggacgca accttcagga
     9241 cgtgttggcg caaatccgca ccgcgcatcc cgaccttacg gcgcaggaaa acggcgcgtt
     9301 gcaggacatc gcctacggtt gccagcgtta tttgggcagt ttgaaacata tgctcgcgca
     9361 gatgctgaaa aaaccgattg acaatccgca gctcgaaagc ctgcttttgg cggcgttgta
     9421 ccaactgcat tacacgcgca acgcgccgca cgccgtggtc aatgaggcgg tggaaagcat
     9481 agccaaaatc gggcgcgggc agtaccgttc gtttgccaac gcggttttgc gccgtttttt
     9541 gcgtgaacgt gaaaaattgg cgtcttcctg caaaaaagac gatgtggcaa aacacaacct
     9601 gccgctgtgg tgggtggctt acttgaaaaa ccattatccg aaacactggc acaacatcac
     9661 cgccgcgctg caatcgcatc cgccgatgac gttgcgcgtc aaccgccggc acggcaatgc
     9721 cgaaagctat ttggaaaaac tggctgcgga aggtatcgcc gcaaaggcgt tggacgaata
     9781 tgcggttacg ttggaagaag ccgtgccggt gaaccgcctg cccggttttt cagacggcat
     9841 tgtttcggta caggacttcg gcgcgcagca ggcggcgtat cttcttaacc cgaaagacgg
     9901 cgaacggatt ttggacgcgt gcgccgcgcc gggcggcaag acggggcata ttttggaatt
     9961 ggcggattgc cgcgttaccg ccttggacat cgatgcaggc cgtctgaagc gtgtggagga
    10021 caatatcgcc cgtttgggct ttcagacggc atcggcggcg tgtgccgatg cgcgggattt
    10081 ggcggcgtgg tatgatggaa agcctttcga cgcaatcctt gccgacgtgc catgcaccgc
    10141 ttccggcgtg gcgcggcgca atcccgacgt gaaatggctg cgccgtccga ccgacgcgct
    10201 caaaaccgcc cgccagcagg aagccctgct ggacgcactt tggcagacgc tgaaacaggg
    10261 cggcagaatg ctgcttgcca cctgttccgt gttcgtcgaa gaaaacgatg ggcaattgca
    10321 aaaattcctc aaccgccatg ccgatgccga accgatcgaa tcgcgggtgc ttttaccgaa
    10381 caaacaccaa gatggctttt attacgcgct tattcaaaag cattaaacaa tggcttgtgc
    10441 tgctgccgat gctttccgtt ttgccggacg cggcggcgga ggggatagat gtgagccgcg
    10501 ccgaagcgag gataaccgac ggcgggcagc tttccatcag cagccgcttc caaaccgagc
    10561 tgcccgacca gctccaacag gcgttgcgcc ggggcgtgcc gctcaacttt accttaagct
    10621 ggcagctttc cgccccgata atcgcttctt atcggtttaa attggggcaa ctgattggcg
    10681 atgacgacaa tattgactac aaactgagtt tccatccgct gaccaaccgc taccgcgtta
    10741 ccgtcggcgc gttttcgaca gactacgaca ccttggatgc ggcattgcgc gcgaccggcg
    10801 cggttgccaa ctggaaagtc ctgaacaaag gcgcgctgtc cggtgcggaa gcaggggaaa
    10861 ccaaggcgga aatccgcctg acgctgtcca cttcaaaact gcccaagcct tttcaaatca
    10921 atgcattgac ttctcaaaac tggcatttgg attcgggttg gaaacctcta aacatcatcg
    10981 ggaacaaata atgcgccgtt ttctaccgat cgcagccata tgcgccgtcg tcctgttgta
    11041 cggactgacg gcggcaaccg gcagcaccag ttcgctggcg gattatttct ggtggattgt
    11101 tgcgttcagc gcaatgctgc tgctggtgtt gtccgccgtt ttggcacgtt atgtcatatt
    11161 gctgttgaaa gacaggcgcg acggcgtatt cggttcgcag attgccaaac gcctttctgg
    11221 gatgtttacg ctggttgccg tactgcccgg cgtgtttctg ttcggcgttt ccgcacagtt
    11281 tatcaacggc acgattaatt cgtggttcgg caacgatacc cacgaggcgc ttgaacgcag
    11341 cctcaatttg agcaagtccg cattgaattt ggcggcagac aacgccctcg gcaacgccgt
    11401 ccccgtgcag atagacctca tcggcgcggc ttccctgccc ggggatatgg gcagggtgct
    11461 ggaacattac gccggcagcg gttttgccca gcttgccctg tacaatgccg caagcggcaa
    11521 aatcgaaaaa agcatcaacc cgcacaagct cgatcagccg tttccaggta aggcgcgttg
    11581 ggaaaaaatc caacaggcgg gttcggtcag ggatttggaa agcataggcg gcgtattgta
    11641 cgcgcagggc tggctgtcgg cgggtacgca caacgggcgc gattacgcct tgtttttccg
    11701 tcagccggtt cccaaaggtg tggcagagga tgccgtctta atcgaaaagg caagggcgaa
    11761 atatgctgag ttgagttaca gcaaaaaagg tttgcagacc tttttcctgg caaccctgct
    11821 gattgcctcg ctgctgtcga tttttcttgc actggtcatg gcactgtatt tcgcccgccg
    11881 tttcgtcgaa cccgtcctat cgcttgccga gggggcgaag gcggtggcgc aaggcgattt
    11941 cagccagacg cgccccgtgt tgcgcaacga cgagttcgga cgcttgacca agttgttcaa
    12001 ccacatgacc gagcagcttt ccatcgccaa agaagcagac gagcgcaacc gccggcgcga
    12061 ggaagccgcc agacattatc ttgaatgcgt gttggagggg ctgaccacgg gcgtggtggt
    12121 gtttgacgaa caaggctgtc tgaaaacctt caacaaagcg gcggaacaga ttttggggat
    12181 gccgcttacc cccctgtggg gcagcagccg gcacggttgg cacggcgttt cggcgcagca
    12241 gtccctgctt gccgaagtgt ttgccgccat cggcgcggcg gcaggtacgg acaaaccggt
    12301 ccatgtgaaa tatgccgcgc cggacgatgc caaaatcctg ctgggcaagg cgacggtatt
    12361 gcccgaagac aacggcaacg gcgtggtaat ggtgattgac gacatcaccg ttttgataca
    12421 cgcgcaaaaa gaagccgcgt ggggcgaagt ggccaaacgg ctggcacacg aaatccgcaa
    12481 tccgctcacg cccatccagc tttccgccga acgtctggcg tggaaattgg gcgggaagct
    12541 ggatgagcag gatgcgcaaa tcctgacgcg ttcgaccgac accatcgtca aacaggtggc
    12601 ggcattgaag gaaatggtcg aagcattccg caattatgcg cgttcccctt cgctcaaatt
    12661 ggaaaatcag gatttgaacg ccttaatcgg cgatgtgttg gcattgtatg aagccggtcc
    12721 gtgccggttt gcggcggagc ttgccggcga accgctgatg gtggcggcgg atacgaccgc
    12781 catgcggcag gtgctgcaca atattttcaa aaatgccgcc gaagcggcgg aagaagccga
    12841 tgtacccgaa gtcagggtaa aatcggaagc ggggcaggac ggtcggattg tcctgacggt
    12901 ttgcgacaac ggcaaagggt tcggcaggga aatgctgcac aacgccttcg agccgtatgt
    12961 aacggacaaa ccggcgggaa cgggattggg tctgcccgtg gtgaaaaaaa tcattgaaga
    13021 acacggcggc cgcatcagcc tgagcaatca ggatgcgggt ggcgcgtgtg tcagaatcat
    13081 cttgccaaaa acggtagaaa cttatgcgta gcagcgatat tttaattgta gacgacgaaa
    13141 tcggcatccg cgacctgctg tcggaaatcc tgcaggacga aggttattcg gtcgcattgg
    13201 cggaaaacgc cgaagaggcg cgcaagctgc gccatcaggc gcgccccgcg atggtgctgc
    13261 tggatatttg gatgcctgat tgcgacggca tcaccctttt gaaggagtgg gcgaaaaacg
    13321 ggcagctcaa tatgccggtg gtgatgatga gcgggcatgc cagcatcgat accgccgtag
    13381 aagcgaccaa aatcggcgcg ctcgactttt tggaaaaacc gatttccctg caaaagctgc
    13441 tgtctgccgt cgaaaacgcg ttgaagtacg gtgcggcgca aaccgaaacg gggcctgtat
    13501 tcgacaagct gggcaacagt gcggcgattc aggaaatgaa ccgtgaggta ggggctgcgg
    13561 tgaaatgtgc ctctcccgta cttttgacgg gcgaggcggg ttcgccgttt gaaacggtgg
    13621 cacgctattt ccataaaaac ggtacgccgt gggtcagccc ggcaagggtc gaatatctga
    13681 tcgatatgcc gatggaactg ttgcagaagg cggagggcgg cgttttgtat gtcggcgaca
    13741 tcgcccagta cagccgcaac atccaaaccg gcattgcctt tattgtcggc aaggcggaac
    13801 accgccgcgt cagggtggtc gcatcgggca gcagggcggc aggttcagac ggcattgcct
    13861 gcgaggaaaa gctggcggaa ctgctgtcgg aatcggtcgt ccgtattccg ccgctgcgta
    13921 tgcagcatga agatattccc ttcctgatac aggggattgc ctgcaatgtg gcggaaagcc
    13981 aaaagattgc gcccgccgca ttcagtgaag aggcacttgc cgcattgacc cgttacgact
    14041 ggccgggaaa tttcgaccaa ctgcaaagcg tcgttgcaac gctgttgttg gaggcggacg
    14101 gacaggaaat cggcgcaggg gcggtttctt cccttttggg gcagaatgtg cctgccgagg
    14161 gagcggaaga tatggtgggc gggtttaatt tcaacctgcc cctgcgcgaa ttgagggagg
    14221 aggtggagcg gcgttatttc gagtaccaca tcgcccaaga aggtcagaat atgagcaaag
    14281 tggcgcagaa agtcggtttg gaacgcacgc acctttaccg caaactcaaa cagctcggca
    14341 tcggcgtttc gcgccgggcg ggggaaaaaa ccgaagaata ggcccggacg gccggttttc
    14401 cggctgcggg cttttgtttt cagacggcat ttggtgcaaa tgccgtctga aatcgtaagg
    14461 ggacggattt tatgacagag gacgaacgtt tcgcgtggct gcaattggcg tttacgccct
    14521 atatcggcgc ggaaagcttc ctgctgctgc tgcgccgttt cggcagcgcg tcaaacgccc
    14581 tgtccgcacc ggcggaacag gtcgccccgg cggtgcggca caagcaggcg gccgaggctt
    14641 ggcgcaatgc ggaaaaacgc gctttggcgc ggcaggcggc agaagcggcg ttggaatggg
    14701 aaatgcggga cggatgccgc ctgatgctgc ttcaggatga agatttcccc gaaatgctga
    14761 cgcaggggct gaccgcgccg ccggttttgt ttttgcgcgg caacgtgcgg ctgctgcaca
    14821 agccttccgc cgccatcgtc ggcagccgcc atgccacgcc gcaggcgatg cggattgcca
    14881 aagatttcgg cagggcgttg ggagagaaag gcattcccgt tgtgtcgggt atggcttcgg
    14941 gcatcgatac cgccgcccat cagggtgcgt tgcaggcgga aggcggcacc atcgccgtgt
    15001 gggggacggg catagaccgc atttatccgc cgtccaataa aaaccttgcc tatgaaatcg
    15061 ccgaaaaagg actgattgtc agcgagttcc ccatcggcac gcgcccgtat gccggcaatt
    15121 ttccgcgccg caaccgcctg attgccgcgc tgtcgcaggt tacgctggtg gttgaagccg
    15181 ccttggaatc cggttcgctg attaccgccg gcttggcggc agaaatgggg cgcgaagtga
    15241 tggcggtacc cggctcgata gacaatccgc acagcaaagg ctgccacaaa ctgattaaag
    15301 acggcgcgaa actgacggaa tgtttggacg acatcctgaa cgaatgcccg gggctattgc
    15361 aaaatacggg tgcttcatca tattctataa ataaggatac gcccgacacg ggccgccgcg
    15421 ccgttcagac ggcatacgcc ccgccgcctg ccgcaaaaat gccgtctgaa ggggcggcag
    15481 gcggcacagc cgtcggcggc atactggata aaatgggttt cgaccccatc catcccgacg
    15541 tgcttgccgg acagttggct atgcctgccg cagatttgta tgccgcactg ttggaattgg
    15601 aattggacgg cagcgttgcc gcaatgcccg gcggcagata ccagcgtatc cgaacttgaa
    15661 cgcactttat attaaggaac acgaatgacc gaagtcatcg cctacctcat cgaacatttc
    15721 caagatttcg atacctgtcc gccgcccgaa gacttgggta tgctgcttga agaagcgggt
    15781 ttcgatacga tggaaatcgg caacaccctg atgatgatgg aagtattgct caacagctcc
    15841 gaattttccg ccgaacctgc cgacagcggc gcattgcgcg tgtacagcaa agaagaaacc
    15901 gacaacctgc cgcaggaagt gatggggctg atgcagtatc tgattgaaga aaaagccgtc
    15961 agctgcgaac agcgggaaat catcatccac gcgctgatgc acattccggg cgacgaaatt
    16021 accgtagata ccgccaaagt gctgaccctg ctgcttttat gggcaaacaa gagcgagctg
    16081 cccgtgttgg tcggcgacga gctgatgagc gcgcttttac tcgacaacaa acccacgatg
    16141 aactgaagcg gcttcagacg gcattatcct gctaacctgc cgtctgaaac gccggcatca
    16201 aaaccaccat ccagagaacg acaaatggcg aaaaacctat taatcgtcga atccccgtcc
    16261 aaagccaaaa ccctgaaaaa atatttgggc ggcgattttg aaatccttgc atcctacgga
    16321 cacgtccgcg acctcgtccc caaaagcggc gcggtcgatc ccgacaacgg ctttgcgatg
    16381 aaataccaac tcatcagccg caacggcaaa cacgtcgatg ccatcgtcgc cggtgccaaa
    16441 gaagctgaaa acatctacct cgccaccgac ccggataggg aaggcgaagc catttcctgg
    16501 catcttttgg aaatcctcaa atccaaacgc ggcttgaaaa acatcaagcc gcagcgtgtc
    16561 gtgttccacg aaatcaccaa aaacgccgtg ctcgatgccg ttgcccatcc gcgcgaaatc
    16621 gaaatggact tggtggatgc acaacaagcc cgtcgcgctt tggactattt ggtcggcttt
    16681 aacctttcgc cattgttatg gaaaaaaatc cgccgcggtt tgagcgcagg ccgcgtgcaa
    16741 agccccgctt tgcgcctgat ttgtgagcgc gaaaacgaaa tccgcgcgtt tgaagcgcag
    16801 gaatattgga cggtgcatct cgacagccac aaaggccgca gcaagttcac cgccaaactc
    16861 gcccaataca acggcgcgaa actcgaacaa ttcgacctgc cgaacgaagc cgcgcaagcc
    16921 gacgtgttga aagaactcga aggcaaagag gccgtcgtta ccgccattga aaagaaaaag
    16981 cgcagccgca atcctgccgc gccgttcacc acttctacca tgcagcagga tgccgtgcgc
    17041 aaactcggct tcaccaccga ccgcaccatg cgtaccgccc aacagcttta tgaaggtata
    17101 gacgtagggc agggcgcgat cggtctgatt acctatatgc gtactgacag cgtgaatttg
    17161 gcggatgaag cgttaaccga aatccgccat tacatcgaaa acaaaatcgg caaagaatat
    17221 ctgccgagtg ccgccaaaca atacaaaacc aaatccaaaa acgcccaaga agcgcacgaa
    17281 gccatccgtc cgacttccgt gtaccgcacg cccgaaagcg tcaaaccctt cctgagcgca
    17341 gaccagttca aactctatca aatgatttgg cagcgtaccg tcgcctgtca gatgacgcct
    17401 gccaaattcg accaaaccac cgtcgatatt accgtcggca aaggcgtatt ccgcgtaacc
    17461 ggacaagtgc aaaccttcgc aggcttcttg agcgtttatg aagaaagcag cgacgatgaa
    17521 gaaggcgaag acagcaaaaa actgcccgaa atgagcgaag gcgataaatt gcccgtggac
    17581 aaactctacg gcgaacaaca ctttaccact ccgccgccgc gctacaacga agccacgctg
    17641 gttaaagccc tcgaagaata cggcatcggc cgcccctcga cctacgccag catcatctcc
    17701 acgctcaaag accgcgaata cgttaccctt gagcaaaaac gcttcatgcc caccgacaca
    17761 ggcgacatcg tcaacaaatt cctgaccgaa cacttcgccc aatacgtcga ttaccacttc
    17821 accgccaaac tcgaagacca gcttgacgaa attgccgacg gcaaacgcca atggattccc
    17881 ttgatggaca aattctggaa accgttcatc aaacaagtgg aagaaaaaga aggcatcgaa
    17941 cgcgccaaat ttaccacgca ggaacttgat gaaacctgcc cgaaatgcgg cgaacacaaa
    18001 ctgcaaatca aattcggcaa aatgggtcgt tttgttgcgt gtgccggtta tcccgagtgc
    18061 agctacacgc gcaatgtcaa cgaaaccgcc gaagaggccg ccgagcgcat cgccaaagcc
    18121 gaagccgaac aggccgaact cgacggacgc gagtgcccga aatgtggcgg tcgcctagtg
    18181 tacaaataca gccgcaccgg cagcaaattc atcggctgcg ccaactatcc gaaatgcaaa
    18241 cacgtcgagc cgctggaaaa accgaaagat accggcgtcc agtgtccgca atgcaaaaaa
    18301 ggcaacctcg tcgagcgcaa atcccgctac ggcaaactgt tttacagttg cagcacctat
    18361 cccgactgca actacgccac ttggaacccg cccgttgccg aagaatgccc gaactgccat
    18421 tggccggtct tgaccatcaa aaccaccaaa cgctggggcg tcgaaaaagt ctgcccgcaa
    18481 aaagaatgcg gctggaaaga acagattgaa ccgcccgcgc cgaaggagta agattaggtt
    18541 ggtttgaaag agaaaaggtc gtctgaaaaa ttttcagacg acctttgctt ttctgtgatt
    18601 ggtttatttg aatccgcgtg ttgttttaaa gtccgataaa atccggttca tttcaggcac
    18661 aaacaaggcg atgtaatcgt aagatagacc gcgactggca ctgggatggg gaaagcagac
    18721 gacttcgcaa tcttcaaacg attggaattt gacattgaaa cgtgtaccgt caaattcttt
    18781 ttgcaccgtc tccagcggtt tggtctgctt accgaccaac tgctcgaagc gtggcagtac
    18841 attttggttg ttcagaaaat ccgccaacct gctgcccatg aagaggatga ctttcggacg
    18901 cagtttttcg atgtggtaga gaaaattatc gatgtgctcg ggttgtgtga acttgtcggg
    18961 attgtcgata gtgttgccct gtgtagcagc ccagttggtt tgaaccaggg atttttcaaa
    19021 tgcaccgccc aatccatttt cgtctaaggg gtgtccccac atttcaaacc aattttttat
    19081 cgtattgtcg taacgccact tttttgcctg ctctccgaaa tagagggatt tgtttgcaaa
    19141 tgtatggtcg attttgtttt cagggagttt gtattcacct gctacataag cagcctcatc
    19201 ggctttactc caaccccatt catagccaca aatcattaag ccatgtttgt cgttgtagcc
    19261 tttgaacagg ctgttgctca aattcaaatc tttcatcatg aactcttcct tttaaaattt
    19321 aagagcgatt gacttcaatg tttttagatg gggtggaaaa atccttgtgt aggcaacata
    19381 aattcaataa atttcttgat aattcgaaac ctactaatag cgcacctata aaagcttttt
    19441 cattacgttc agcatgacgg tcacgtcgtt catatttttt acgcttgctg ttccctgtta
    19501 ttacagctaa gccaagtgat atggcgagaa ttgcccaaac aatagtactt aataacaatt
    19561 ttccccatac tatcaatagg gaaagaaaaa accttttagt attaggtcga tagattataa
    19621 tccatgccca tgaaaatgca ataagagtta tacataagat acaagctgat aggatttctt
    19681 tatttttaat taaataactt agggcgatga ggatgacagg tgtcaagaaa acaatagtta
    19741 catcccgata gctataaaag aaaactgccc cattttgaat gtggaaatgt gcacagaatc
    19801 caatatagct aaggataata gttaatataa taaaaaaaga ccaccaaggg tgaagagata
    19861 ggaattccat gttttccctt tttttgtaaa aaggaaaaaa tcttatctaa tagttaattg
    19921 cctaaccagc cagaagaagt ttaaaatcta tcccaataat tcaaccatct atacagaaag
    19981 ttcagcttat ggaaacccac gaaaaaatcc gcctgatgcg cgaattgaat aaatggtccc
    20041 aggaggatat ggcggaaaag ctggcgatgt cggcaggcgg gtatgccaaa atcgaacgag
    20101 gcgaaacgca gttgaatatc ccgcgtttgg agcagttggc tcagattttc aaaatcgata
    20161 tgtgggactt gctcaaatcg ggcggtggtg ggatggtgtt tcagattaat gaaggtgata
    20221 gtggtggcga tattgcgttg tatgcgtcgg gtgatgtttc gatgaaaata gaatttttaa
    20281 aaatggagtt gaaacactgc aaagaaatgt tggaacaaaa agacaaagaa atcgagctgc
    20341 tccgcaagct gaccgaaacc gtttaaacag atatgccgtc tgaaaaaagt tttcagacgg
    20401 catattcttt gacaggtctt gtataatacc gtttgaactt acaggttttt gattatggcg
    20461 gcaggcaaac ataccaaaca cagcaaccgg gtacgcatta tcggcgggca atgccggggc
    20521 aggaaattga gtttcacatc cgccgacgga ctgcgtccga cacccgacag cgtgcgtgaa
    20581 aagctgttta actggctggg acaggatttg acgggtaaaa cggttttgga tctcttcgga
    20641 ggcagcggcg cactcggtat agaagccgct tcgcgcaacg ccaaacgcgt gctgatttcg
    20701 gataacaacc gccaaaccgt gcagaccttg cagaaaaaca gtcgcgaact gggtttgggg
    20761 caggtgcaaa tcgtcttttc agacggcatc gcatatttga agaccgtatc cgaacagttt
    20821 gatgttgtct ttctcgaccc gccgtttgca tggcaggact ggcaaatcct gttcgatgcc
    20881 ttgaagccgt gcctgaaccc ccgggcattc gtctatctcg aggcgggtac gctgccgaat
    20941 attcccgatt ggctgacgga atatagagaa gggaaatcgg ggcagagtac attcgaatta
    21001 agggttttcc aagtggctga ataatatgcg ctttgataat catttccgag ttgtaaacat
    21061 tcgtttgcaa ccgtccggtt caaaaaaacc ttgtgctata atccgcgccc gcccggtttt
    21121 gataatttag tggaaaagga aaagaaatgt cgctttttat taccgacgag tgcatcaact
    21181 gcgacgtatg cgaacccgaa tgccccaatg atgccatttc ccaaggcgag gaaatttacg
    21241 aaatcaaccc caacctctgc acgcagtgcg tcggacacta cgatgagccg cagtgccagc
    21301 aggtttgccc ggtggactgc atcctgattg acgaagaaca tcccgaaacc catgacgagt
    21361 tgatggcgaa atacgaaaag attatccagt ttaaataaat tctttttaaa acatcaaatt
    21421 atgtctgttt tgaaataaaa tcaaaaaaaa acttgacgga aaagcaagcc gctaataaac
    21481 taacgttctc ttttggaggg attcccgagc ggtcaaaggg ggcagactgt aaatctgttg
    21541 cgaaagcttc gaaggttcga atccttctcc ctccaccaaa attcttactt ggggcagtag
    21601 cgagtaatgc gggtgtagct caatggtaga gcagaagcct tccaagctta cggtgagggt
    21661 tcgattccct tcacccgctc caaacaatta ggcccatgta gctcaggggt agagcactcc
    21721 cttggtaagg gagaggtcgg cagttcaaat ctgcccatgg gcaccatccc tcgattattc
    21781 atttctttaa ggcttagata tataggatat tg
//
LOCUS       DAWHXS010000026        21547 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWHXS010000026 DAWHXS010000000
VERSION     DAWHXS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21547
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.026"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            112..270
                     /locus_tag="WIE22_000746"
     CDS             112..270
                     /locus_tag="WIE22_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126871.1"
                     /translation="MPSEHAFGRRFYNKNTSWRLVLCLSKFLCRAYLRISDGIGLCCL
                     KSVFRFSL"
     gene            352..1473
                     /gene="dnaJ"
                     /locus_tag="WIE22_000747"
     CDS             352..1473
                     /gene="dnaJ"
                     /locus_tag="WIE22_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ4126872.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1669..3687
                     /locus_tag="WIE22_000748"
     CDS             1669..3687
                     /locus_tag="WIE22_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ4126873.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
     gene            complement(3730..4287)
                     /gene="rfbC"
                     /locus_tag="WIE22_000749"
     CDS             complement(3730..4287)
                     /gene="rfbC"
                     /locus_tag="WIE22_000749"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ4126874.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLPLSR"
     gene            complement(4341..5207)
                     /gene="rfbA"
                     /locus_tag="WIE22_000750"
     CDS             complement(4341..5207)
                     /gene="rfbA"
                     /locus_tag="WIE22_000750"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ4126875.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            complement(5313..6353)
                     /gene="rfbB"
                     /locus_tag="WIE22_000751"
     CDS             complement(5313..6353)
                     /gene="rfbB"
                     /locus_tag="WIE22_000751"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ4126876.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEF
                     IQTNIVGTFNLLEAARAYRQQMPSEKHEAFRFHHISTDEVYGDLSGTDDLFTETAPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            complement(6354..7397)
                     /gene="galE"
                     /locus_tag="WIE22_000752"
                     /pseudo
     CDS             complement(6354..7397)
                     /gene="galE"
                     /locus_tag="WIE22_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(7390..8392)
                     /gene="dcm"
                     /locus_tag="WIE22_000753"
                     /pseudo
     CDS             complement(7390..8392)
                     /gene="dcm"
                     /locus_tag="WIE22_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(8423..10696)
                     /locus_tag="WIE22_000754"
     CDS             complement(8423..10696)
                     /locus_tag="WIE22_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ4126877.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPVRTMPGHRA
                     LAVLRGRNEGVLNIALKYRPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYKNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(10761..11411)
                     /locus_tag="WIE22_000755"
     CDS             complement(10761..11411)
                     /locus_tag="WIE22_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4126878.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(11408..12205)
                     /locus_tag="WIE22_000756"
     CDS             complement(11408..12205)
                     /locus_tag="WIE22_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ4126879.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(12205..13368)
                     /gene="ctrB"
                     /locus_tag="WIE22_000757"
     CDS             complement(12205..13368)
                     /gene="ctrB"
                     /locus_tag="WIE22_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ4126880.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(13383..14558)
                     /gene="ctrA"
                     /locus_tag="WIE22_000758"
     CDS             complement(13383..14558)
                     /gene="ctrA"
                     /locus_tag="WIE22_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ4126881.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            14693..15826
                     /gene="neuC"
                     /locus_tag="WIE22_000759"
     CDS             14693..15826
                     /gene="neuC"
                     /locus_tag="WIE22_000759"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ4126882.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            15830..16516
                     /gene="neuA"
                     /locus_tag="WIE22_000760"
     CDS             15830..16516
                     /gene="neuA"
                     /locus_tag="WIE22_000760"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ4126883.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            16517..17566
                     /gene="siaC"
                     /locus_tag="WIE22_000761"
     CDS             16517..17566
                     /gene="siaC"
                     /locus_tag="WIE22_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ4126884.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            17718..20831
                     /locus_tag="WIE22_000762"
     CDS             17718..20831
                     /locus_tag="WIE22_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4126885.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLSPPFHLHPNVKTSSIIDLFNDIPENVSCRNIPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSEEDSIIFTHPLQLEMYRLANNNIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSQSEGFGYIFLEGMVYDIPILAYNFKYGAND
                     FSNYNENASVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKNSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
     gene            20912..21547
                     /locus_tag="WIE22_000763"
     CDS             20912..21547
                     /locus_tag="WIE22_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ4126886.1"
                     /translation="MYSEQGINNTINISTTSLTNATQLTVIGNNNSVYIGNNCKIVSS
                     NIRLKGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIAEKGKISIGKD
                     CMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILKGVCIPNNVVV
                     GSHTVLYKSFKEPNCVIAGSPAKIVKENIVWGRKMYHSTMYDDPTLNEFYK"
BASE COUNT         5872 a         4856 c         4905 g         5914 t
ORIGIN      
        1 gggaacctgt aaaggaaggg gcatcggctg ccgccggtgc ttttttgttt ggaagggaaa
       61 tggctggaaa aagacctgcg ctatatcggg gaatgaatcg acgcaaagga aatgccgtct
      121 gaacatgcgt tcgggcggcg tttttataac aaaaacactt catggcggtt ggttttatgc
      181 ctgtctaagt ttttgtgtcg tgcatacctg aggatttcag acggcatcgg tttatgctgt
      241 ctgaaaagtg tattccgttt cagtttgtaa gctatggcag tctgtttgtc ttgtgttttg
      301 cgtaattgcc cttattttga gccgtgattt tattttgaat tagatgaaaa aatgagtaat
      361 caagattttt atgcgacgct gggtgtggca agaacagcta ccgatgatga gattaaaaaa
      421 gcctaccgga aattggcgat gaaataccat cccgaccgca atcctgacaa taaagaggcg
      481 gaagagaagt ttaaagaagt acaaaaggcg tatgaaactt tgtccgacaa ggaaaagcgc
      541 gctatgtacg accagtatgg tcatgcggcg tttgaaggcg gcggacaggg gggcttcgga
      601 gggtttggcg gatttggcgg tgcgcagggt tttgactttg gggatatttt cagccaaatg
      661 tttggaggcg gttcggggcg cgcccagcct gattatcagg gtgaggacgt tcaagtcggt
      721 atcgaaatca cgcttgaaga agccgcaaaa ggtgtgaaga aacgcatcaa tattccgact
      781 tatgaagcgt gtgatgtctg taacggcagt ggcgcgaaac cggggacatc cccggaaacc
      841 tgcccgactt gcaaaggttc gggtacggtg cacatccagc aggcgatttt ccgtatgcag
      901 cagacttgtc cgacctgcca cggtgcgggc aaacacatta aagaaccttg cgtcaaatgc
      961 cgtggcgcgg ggcggaataa ggcggtcaag acggtggaag tcaatattcc cgccggtatc
     1021 gatgacgggc agcgtatccg tttgagcggc gaaggcgggc cgggtatgca cggtgcgcct
     1081 gccggcgact tgtatgtaac cgtccgcatt cgggcgcata agattttcca acgcgacggt
     1141 ctggacttgc attgcgaact gccgatcagt tttgccacgg ctgctttggg cggggagttg
     1201 gaagtgccga ccttggacgg aaaggtcaag ctcaccgtcc ccaaagaaac ccaaaccggc
     1261 aggaggatgc gtgtgaaagg taagggtgtc aaatcgttgc gcagcagcgc gaccggcgat
     1321 ttgtactgcc atattgttgt cgaaacgcct gtcaatttga ctgaccgtca aaaagagctt
     1381 ttggaagagt ttgagcggat ttctaccggc ttggaaaacc aaacaccgcg caagaaatcg
     1441 tttttagaca agctgcgcga tttgtttgat tgattttaag gttcggaaac aagcagccgt
     1501 atcggggaat ctccttgata cggctgtttt tatttgttta aaaatagttt ttattttcaa
     1561 tggggtatga ggcagggtgg gataactgtt tttaactgtt ctttttaaaa cttgacatca
     1621 tggcgtgatg ccaacaatat gtgaacgtct gttgtcaaag gaagaataat gaataaatct
     1681 ttatccagtt cggtagaaga ataccgcgag ctgacgctcc gaggcatgat actcggtgca
     1741 ttgatcactg taatttttac tgcgtccaat gtttacctcg gtttgaaagt cgggctgacc
     1801 tttgcctcgt cgattccggc ggcggtgatt tcgatggcgg ttttaaagtt tttcaaaggc
     1861 agcaatattt tggaaaacaa catggtgcag acccaagctt cggcggcggg tacgctttcg
     1921 accatcatct tcgtcctgcc cggtttgctg atggcgggct actggagcgg tttcccgttc
     1981 tggcagacga cgcttttatg tattgccggc gggattttgg gggtgatttt caccattccg
     2041 ctgcgttacg cgatggtggt gaaaagcgat ttgccttatc cggaaggtgt ggcggctgct
     2101 gaaattttga aagtgggcgg tcatgaagaa ggggataacc gtcagggcgg cagcggcatc
     2161 aaagagctgg cggccggcgg tgcgttggcg ggattgatga gcttttgcgc cggaggtctg
     2221 cgcgtgattg ccgacagcgc gagttattgg tttaaaagcg gtacggcgat tttccagctg
     2281 ccgatgggct tttcactggc attgttgggc gcgggctatt tggtcggact gacgggcggt
     2341 atcgccatcc tgttgggcat ttcgattgct tggggcattg ccgtgccgta tttctcctca
     2401 cacattccgc aaccttccga tatggaaatg gcggcgtttg cgatgaagct gtggaaggag
     2461 aaagtgcgtt ttatcggtgc ggggactatt ggcattgcgg cggtttggac gctgttgatg
     2521 ctgctcaagc cgatggtgga aggcatgaag atgtcgttca agagttttgg cggcggtgcg
     2581 cccgctgcgg aacgcgccga acaagatttg tcgcctaaag ccatgatttt ttgggtgctg
     2641 gcgatgatgt ttgttttagg cgtgtcgttt taccacttta tcggcgattc gcacattacg
     2701 ggcggcatgg cttggctttt ggtggtcgtt tgcacgcttt tggcttccgt catcggcttt
     2761 ttggtcgccg ccgcctgcgg ttatatggca ggtttggtcg gctcgtcttc cagcccgatt
     2821 tccggcgtgg gcatcgtgtc cgtcgtcgtt atttcactgg ttttgctgct ggtaggcgaa
     2881 tccggaggtt tgttggcgga tgaggctaac cgcaaatttt tgctggcact gactttgttt
     2941 tgcggctcgg cagtaatctg cgtggcttcg atttccaatg acaacctgca agacttgaaa
     3001 accggctacc tgctcaaagc cacgccttgg cggcagcaag tcgccctgat tatcggctgt
     3061 atcgttggtg cgctggttat ttcgcccgtg ttggaactgc tttacgaagc ctacggcttt
     3121 accggcgcaa tgccgcgcga aggcatggat gcggcgcagg ctttggcagc tcctcaagcg
     3181 actttgatga cgaccatcgc gtcgggcatt ttcgcccaca acctcgaatg ggtctacatc
     3241 tttaccggca tcgcgatcgg cgcggtatta atcgtcgtcg atttggtgtt gaaaaaatca
     3301 tcaggcggca aacttgccct gcccgtcctt gcggtcggta tgggtattta tctgccgccg
     3361 tccgtcaata tgcccatcgt ggcaggcgcg gtgttggcgg cggtgttgaa acacatcatc
     3421 ggtaaaaaag cggaaaaccg cgaaggccgt ctgaaaaacg ccgagcgtat cggaaccttg
     3481 ttctccgccg gcctgattgt cggtgaaagc ctgatcggtg tgattatggc gtttattatt
     3541 gccttctccg tgaccaacgg cggctcggat gcgccgcttg cgttgaatct gcaaaactgg
     3601 gatgccgccg cttcttggtt gggtttggcg ttcttcgtta ccgggatgtt tttctttgca
     3661 cagcgcgtac tgaaggcggg caagtaggct gtcggaaaaa atgccgtctg aagcgttcag
     3721 acggcatttt tatctggaaa gcggaaggcg gagcttttcg gcttgcgccc acgttttgcc
     3781 ggcaaggtct ttgggcgaca gcagcggcgc ggtttgaagc ggccagccta tgccgactgt
     3841 cgggtcgttc catattaaaa cctgttcggc ttcaggcttg taatagtcgg tgcatttgta
     3901 cacgacttcc gccgcgtcgc ccaaaacgca aaacccgtgc gcgaagcctt cgggtatcca
     3961 aagctggcat tggttttgcg cggacagggt tgcgcccgcc catttgccga aagtcggcga
     4021 accttcgcgc atatcgacgg ccacgtcgaa cacttcgccg acaactacgc gtacgagttt
     4081 gccttgtgtg ttttcggttt ggtagtgcag gccgcgcaat acgcctttgc cggatttgga
     4141 gtggttttcc tgcacgaagg tgcgttcgca gacttgggtt ttaaaccact cgtcgcggaa
     4201 ggtttccata aaaaagccgc gcgcgtcgcc gaagacttgg ggctcaagca gttttacgtc
     4261 aggaatggcg gtatcaatga tgttcatctt tttatctttc atctaaaggc cgtctgaaaa
     4321 gtttcagacg gcctcaaaca ttattttttc aacaggcgca gcaaatattg gccgtattgg
     4381 tttttcgcca tcgggcgcgc caattcttcc agtttttcat cggaaagcca accgttgcgc
     4441 caagcgattt cttccaggca ggcgatgtgc aggttttgga tgttttgcac ggtttggacg
     4501 aacgaagccg cctcgtgcag gctttcgtgc gtaccggtgt ccagccacgc gaaaccgcgt
     4561 cccaatattt gaacggagag cgagccgtct tccaaataca tccggttgag gtcggaaatt
     4621 tccaattcgc cgcgtgcgga cggtttgagc tgtttggcga actcgacggc gcggttgtcg
     4681 tagaaataca agccggttac cgcccaatcg gatttgggcc gttgcggttt ttcttcgatg
     4741 gaaacggcgc ggaagttttc gttaaattca accacgccga aacgttcggg gtttttgacc
     4801 tgataagcaa acacggttgc gccgtgcgtt tgcgctgccg cctgtttcaa tgtttgcgta
     4861 aacgactgac cgtaaaaaat attgtcgccc aaaaccaagc aaacattgtc gttgccgata
     4921 aattcttcgc cgatgataaa tgcctgtgcc aagccgtccg gactgggttg cacggcataa
     4981 ctgatggaaa tgccgaaatc gctgccgtcg ccaagcaggc gtttgaaaga ggcgttgtct
     5041 tcaggcgcgg taatcaccaa aatatcgcgg attcccgcca gcatcaaaac cgacaagggg
     5101 taataaatca tcggtttgtc gtacacgggc aggagctgtt tggatacgcc gcgcgtgatg
     5161 gggtagaggc gcgtgccgct gccgcctgcc agtatgatgc ctttcatctt ttctttcttc
     5221 ctttgcgatg ggttttcaga tggcattgcg tcgggatgcc gtctgaaaac ttattttccg
     5281 gtgcctaaac gttccaaacg atagctgccg ttttatgcgt tttgccgccg agttttgttg
     5341 tccaagtacc attgcactgt tttgcggagg ccggattcaa aggtttccaa aggctgccag
     5401 cccaaatccc gtctgatttt cgccgtatcg acggcgtagc gggcatcgtg gccagggcgg
     5461 tcttgtacga aagtaatcaa atcttcataa cgcgccacgc cggccggttt ttcgggagcg
     5521 agttcttcca gcagggcgca gatggttttg acgacttcaa tattggcttt ttcattgtgg
     5581 ccgccgatat tgtaggtttc gccgacaaca ccttcggtaa caacctgata cagtgcgcgc
     5641 gcgtggtctt cgacaaacag ccagtcgcgg atttgcatac cgtcgccgta cacaggcagc
     5701 ggtttgccgt caagcgcgtt cagaatcatc aaaggaatga gtttttccgg aaaatggtaa
     5761 ggaccgtagt tgttggagca gttggttaca atggtcggca agccgtaagt acgcaaccac
     5821 gcgcggacga ggtggtcgct ggacgcttta gaggcagagt aagggctgga cggcgcgtag
     5881 ggcgcggttt cggtaaacaa atcgtctgtg ccgcttaaat cgccataaac ttcatcggta
     5941 gaaatatggt ggaaacggaa ggcttcgtgc ttttcagacg gcatttgctg ccggtaggca
     6001 cgcgcagctt caagtaggtt gaatgtgccg acgatattgg tttggataaa ctcgcctgcc
     6061 gaaccgatag agcggtcgac atggctttcc gccgccaagt gcatcacggc atcgggccgg
     6121 tgttgtgcga atacgcggtc gagttcggcg cggtcgcaaa tgtccacttg ctcgaaagca
     6181 tagcggggat tatcggcaat gtcggtcagc gattcgagat tgcccgcgta ggtcagtttg
     6241 tcgaggttga cgacggaatc ttgggtgttt cggataatat gacggacaac ggcagagccg
     6301 ataaagcccg caccgccggt aacgaggatg gtttttttgt ttgcggtttg catttagtcc
     6361 ccgtatccgt cggggttgcg gctgacccaa cgccacgaat cctccatcat ttgtttcaaa
     6421 ccgcgtttgg tttcccagcc cgtctcttgt ttggtatggg acgggtcggc aaaggaacac
     6481 gccaagtctc cggcgcgtcg gggttggatt tgatagggaa tatttaagcc ggatgcggcc
     6541 tcaaaggcac ggatgatttc taagacggaa taggcgcggc ccgaacccaa gttgaacaaa
     6601 tgtacgccgg aaaggccgcc tttcgccttc attgccgcga tatgcccttc tgccaaatcc
     6661 atcacatgaa tatagtcacg catccccgtg ccgtcggggg tagggtagtc atcgccaaat
     6721 accgccaatt gcggcagttt gcctgccgcc acttggcaga tataaggcaa caaattattc
     6781 gggatgccgt ttggctgctc gccaatcaaa ccactttcgt gcgcgccaat cggattgaaa
     6841 taacgcaaca aaatcacgct ccagcgcgga tcggcctttt ggatgtcggt taacatccgt
     6901 tccaccatcg ctttggatgc gccataagga ttagcggtat cgcccgggcg catatcttcc
     6961 gtatagggca ttttacgtat catggcgcga cgcattaaag taaacgccaa agcgcgttta
     7021 tactggttat ccagtttccc tatattttta cgttacaaat caagttgttg gtattcatcg
     7081 tgaaaataga atttttcggc ataacgctga ctcataaaac cggcatgcgg gctacctgaa
     7141 aaaatcattg cgccatcatc gtcatttaat gtttcatggt tgttttctat taatgctaaa
     7201 gcaatttggt aattaattgc caaaatatcg ttagtaatca cacgatagcc gcgttttttg
     7261 gcttcgtagg caaaggaaca gcctccacta aatacatctg caactgtatc tacatcagac
     7321 ggaagctggt cacaaatcca ggaagctatt ttctctttat taccgatata gttaattttc
     7381 ggatattgct tattcatttt cactcatttt taaaatactt tcagcaatag cttttgccaa
     7441 taaaggcggt acggcgttac caacctgctg ctgctgggca attttgctgc cgcaaaaaat
     7501 aaaattatca gggaaagatt gtaaggcagc taattcacga acggttaacg cccgattctg
     7561 ttcatagtga aaaactttgc gcatatctcc tgtaatacaa acggctggtt ttgttgctgt
     7621 tgtaacggat gtatttacgg atatcacctg ttttcggacg taatggttca ggaatatcgt
     7681 tacggttacc tccattttta acaaatgcca ttttttctaa catttgtgcc gaatgattca
     7741 tagcttcatg atttgcaacg tgtggattgc tttcgccagc agccagtttt ggaaaatgtc
     7801 ctattgctga tccaacagtc tgatgggaaa tctgcaaagg ttcgggaaag gaaattttgc
     7861 ctttatccct cctcccgata aatatcactc ggctacgtat ctgaggaaca ccgaaatcgg
     7921 ctgcactcag tatcttacat tccaccgaat aaccgatatt ctgaaatgct tgaataatct
     7981 caatacgtgt tttacctgaa ttgtgtgtat agagtcgcgc tacattttcc ataacaaaaa
     8041 aatatggttg gacaatttta actattcgga caaactcttt aaataaatgg ttgcgtgggt
     8101 catctgtaaa tgtccgtcca atctttcctg ccatactaaa accttgacaa ggtggtcctc
     8161 caataatcaa atcaactgct tgtccgttaa gacaattgat taaatcttgt tcggttagtg
     8221 tggttaaatc tttttgcagt aattgatgat gggggaagtt ggtacggtaa gtctgacaat
     8281 aatcagactc catttcaaca gaaagcaatt gttggaatcc ggcttgttca aaacccaagg
     8341 ataggcctcc tgctcctgag aaaaggtcaa tataggtaag tggtgtgtgc ataagtcaaa
     8401 atccataaac tcttcgatta ttttaccgct tcagcttcgc aaacgcatcc gccatcgccg
     8461 aattggctgg ggcgcggtcg ttgcgttggg gtttgcggcc ggctgtccgt tcgcggctgc
     8521 ggttttcaga cggcatttta tgttttgcgc cgcccggttc gtcatccaag cgcatggtca
     8581 gcgcgatgcg tttgcgtgca gcatcgactt ccagcacttt cactttcacc acgtcgccag
     8641 ctttcaccac ttcgcgcggg tcttggacga acttgttgga cagggcggag atgtgcacca
     8701 agccgtcctg atggacgccg atgtccacga acgcgccgaa gttggcgacg ttggaaacca
     8761 cgccttcgag tatcataccg acttgcaagt cgctgatttc gtggatacct tcggcaaacg
     8821 atgccgtctg aaactcgccg cgcggatcac ggccgggttt ttccagttcg gacaggatgt
     8881 ccaaaatggt cggcaggccg aagcgttcgt cggtgaagtc ggacgctttg atttgcttca
     8941 cgcgctcgcg gttgccgatg agttcggcgg cgctaatgcc ttgttgcgcc agcattttgg
     9001 cgacgacggg ataggcttcg gggtggacgg cgctcgcgtc caacggctct ttgccgccgt
     9061 taatccgcaa aaagcccgcc gcctgctcga aggttttttc gcccaaacgc ggtactttca
     9121 gcaatttttt gcggctgtcg aacgcgccgt tttcatcgcg gtaggcgaca atgttttggg
     9181 caagggtttg attcaaaccg gaaattcgcg ccaagagcgg ggcggaggcg gtgttcacgt
     9241 ccacgccgac ggcgttcacg cagtcttcga ccaccgcgtc cagcgatttg gcgagctggc
     9301 tttggttcac gtcgtgctga tactgcccca cgccgatgga tttggggtcg attttgacca
     9361 actcggcaag cgggtcttgt agcctgcggg cgatggacac cgcgccgcgc agggaaacgt
     9421 ccaagtcggg gaactcgcgc gccgccagtt cggacgcgga ataaatcgac gcgccggctt
     9481 cggacacgac gattttatgc agccccattt tcgacatttc acgcaccagt tcgcccgcga
     9541 ttttgtcggt ttcacggctg gcggtgccgt taccgatggc gatgagcttc acgccgtgct
     9601 gtttaatcag gcgcgacagc gttgccaaca tattgttttc ttgatgcaaa tagacgatga
     9661 cggtatccag cagcttgccc gtgtcgtcca ccacggcaca tttcacgccg ttcttgtatc
     9721 cagggtcgag acccagcgtg gtcagccgtc cggcgggtgc ggcgagcagc aagtctttga
     9781 gatttcgggc aaacacggta atcgcgtcgg tgtcggcggc ttctttcaaa cgattgagag
     9841 cttcgagttc caacgacaaa aagatttttg cgcgccaagt cagacgcacg gtgtcgcgca
     9901 gccatttgtg gccgtctgaa accttgaaac ggcgggcgat gatttgctcg tattcgcttt
     9961 gctgcgtaat cggcgtgtcg tcgggccggt atttgagcgc gatgttcaac acgccttcgt
    10021 tgcggccgcg caaaaccgcc aacgcgcggt ggccgggcat agtgcggacg ggttcgcggt
    10081 ggtcgaaata atcgctgaat ttttcgcctt cggtttcttt gcctccaaca acttgcgcgt
    10141 ggatttcggc ttcgttccac agcttgtcgc gcagcgtgcc gatgagttcc gcgtcttcgg
    10201 caaactgctc catcagaatc gcacgcgcgc cgtccaacgc ggctttggca tcggggacgt
    10261 tttcgttcag gtagccttgt gcggcggctt ccacgtcctg cgactgctcg gcaagcaaca
    10321 cgtccgccag cggctgcaaa ccgtgttcgc gcgcgatttg cgctttggtg cggcgtttgg
    10381 gtttgtaggg cagatacagg tcttccagcg cggttttgtt atcggcggct tcgatttgcg
    10441 ccctgaggtc gtctgaaagc ttgccttgct cttcaatgct ttttaaaaca acggctttgc
    10501 gctcttccaa ctcgcgcagg tattgcagcc gctcggcaag ccggcgcagc tgcgtatcgt
    10561 ccaacccgcc cgtggcttcc ttgcggtagc gggcgataaa gggcacggtt gcgccgtcgt
    10621 ccaaaagctc gatagcggca ttgatttgcg cggtagtcgc ggaaagttct tgggaaagaa
    10681 tttgagtaat gttcatcaat aaaattccaa cggacaggcc gtctgaaatt tcagacggcc
    10741 tgatttaaaa aacaatcgct ttaaggtagc gaattataat attcgtaggc tttgtccata
    10801 tcttcaaact ggtgcatatg ccctttttcc agcaccatcg cattatcgca atattgcttc
    10861 atggcgctgt ggctgtgcga caccaagatg atggaacggt ctttgcgctt ttcaaacaac
    10921 tcgtacttac atttatcggc aaagcgcgag tcgccaactg caatcacttc gtcaatcaga
    10981 tagcaatcaa actctaccgc caacgacaaa gcaaatgcca aacgcgcctt catacctgaa
    11041 gaataacgct tgaccggctc gtaaagatat tgccccaact cggaaaactc ttcggtaaag
    11101 ttcttcacat aatcgatatc gacattgtaa atccggcaga tgaaacgcaa attgtccata
    11161 ccggtcagac tgccttgaaa cgcaccggag aatgccaaag gccaagaaat actcattgtc
    11221 cgcttgattt cacccgtggt cggcggctca acgccactga tcaaacggat gagcgtcgat
    11281 ttacctgcac cgttgcggcc gagaataccg attttctcgc ccttctccat tttgaagcta
    11341 atatcgtgca agactgtccg ccaaccttgg cgggtcagat agcgtttgga aacgtgttca
    11401 actgaaatca ttgcggctcg actcctttac tgaatttact gaccatcgcc aagccaaaca
    11461 acaacaacac cagattgcac aatacgatat accaaggatt ttcataggta attacatcgc
    11521 tgccaaaata tccggcacgg aacatttctg tgccatgcac catcggaatc attaatgcat
    11581 attcttgtac cttgggcggc aaattatgca caaaaaagaa cgcaccggat aacggcatca
    11641 tcacaaaagt caatgtgccc caaatcttgc caaacggctc gaaattaaag gcaatcgaac
    11701 aaatcaccaa acccaaacca atcgcaaaaa aagccatcaa aagccaagcc atcagcatat
    11761 aaaacatatc tgccggcatt tcaatccagc caatcgcaat caataccgcc ataatcacaa
    11821 tctgcgcaat ggttgcacca gcaatttcca aaatcatgcg cgccaagatg gtatccaaaa
    11881 ctcttacatt gcggtgataa agcaagctgg catttgaaga aatcgaccca actgcccgtt
    11941 ttgaggcatt acgccacatc atcaacatcg gatagccagt aatcgcaaat gcgacaatat
    12001 tcaaagttga atatcggtct gcccttaaaa atttccacat caagacgata acgaatgtca
    12061 tcagcaacgg ctcaacaaac agccataaaa agccaatatt attgcgaccg taacgggtga
    12121 taatttcccg catcaacagc gcaccgatta cgcgcctttg aatggctaaa gattcccaaa
    12181 atgatgtttt atgcaaggct ttcatcagtt tttatgctca cgaatgctgg cagtcaacag
    12241 gctcaaaata ccataaacca tcaagccgat aatcagagtg gcaacaatgt tgtataaccg
    12301 tttaggctca tgtgccaaat ccggcaggct cggttgcgag atcacttcca aataaagctg
    12361 ctgacggtct gcttcaacct tggcactttc caaagaagtc atggcggctg ccaactgctg
    12421 ctctgccaac tggttttcca aatacacacg ctgatattcg gcagcctgat tagacaacga
    12481 agaatgccca ccgcccgaaa tggcacgtaa ctgttggtca atttctttac gcaagctctg
    12541 ctcacgcgcc tgcaaacccg gaatctgcgg attctccgga gtgactgctt tcacctgatc
    12601 cagctgggtt tgaatcacaa tcaattcatc ttgcagcttg gaaaccaacc ccatttgcac
    12661 ttccgattgc gctttcaaat caaaaacgcc attggcaatc cggtaatccg tcagattctg
    12721 agaggcttcc tttacccgct ctgccgccgt tttcactact tcttccgcat agcgcaccgt
    12781 atcagcacgt gcacgatcgt tcaactggtt aatcaatgct tcaccttgtt ttaacaaagc
    12841 ctcattgatt ttcttagatt ccagcgcatc aaaggaagtt acattcaacg tggaaatacc
    12901 cgaaaccgta tcaaaattga tcatcacctg atttttatag tattgataaa aagcctcttc
    12961 ctcgccacgg aacccaaacc cattaaagcg gctgaacgca tcacctttgg tttcataaaa
    13021 ctcacgcacc ggcaagattt tacgcagttc atccaaagac gagcgcgaac gcatatactc
    13081 cccaaccgtg taaatatcat cttgcgcacg ggcaaaacct gtgccctgca aaatggcacc
    13141 caggccattg agagaagatt ggcttttagg cgagcgcacc acaaagctcg attgcgacgt
    13201 aaaacgatcg gaagcgaaga agccgaaata caccaacgaa attaccgtag ggataatcac
    13261 cgttacccaa aataaagggc ttagcttttt aatccaactt ttctttttcg gctttttacg
    13321 ctcggctttg gtttcagtgg caactgccac aggaagttgc tcagacatct taattactca
    13381 cattaattag ttaaattatt aatactgttc gcgccactgg taaccggcga gaacacaaac
    13441 gacaaaaatt tctgcacttc agccaacggc gcattcgaca catacaatac atctttattc
    13501 ttcacaggaa agcgctgcat agaaaatagc gaatgcgcat cagccatatt cacacgatat
    13561 accgttggaa tctctgcctc actgccataa ccttgagcaa tccatttatc ctgacgttct
    13621 gccggcaatt ccaccaatgg cgtatagcgg aacacaaaca caccacgcgc atcagaacgg
    13681 cgatcttgca aaccgcccat acggccaatg gcttcagaaa gcgataagcc tctggctgaa
    13741 aaaccgattt cttgtgttct ccccaccgca cccatagacg taaaggtata gggattggta
    13801 atcatggtaa ccacatcacc gcgacgcagc aaaatatttt gtcgcggatt tgcaactaaa
    13861 tcttccaagg caacagttcg tactacattg ccacgtgtca gctgcacatt cgtatcctgc
    13921 acatttgccg ttgaaccacc taccgcagcc accgcatcca acacacgctc accggctgcc
    13981 gtcagcggca tacgcacact attcccagca cgaatcaccg acacattcgc cgcattattc
    14041 tgcaccaaac gcaccatcac ttgtggctga ttggccattt ttttcaggcg gcctttaata
    14101 atttcctgaa cctgaccagg cgttttaccg accaccgaaa tatcgccaac aaacggcaca
    14161 gaaaccgtac cacgtgccgt gaccaactgc tctggcaact tagtttgatg cgcactaccc
    14221 gagcccatcg aagaaaggcc accaccaaac aatactgccg gcggcgcttc ccaaatcata
    14281 atatccaata catcaccaat atttagcgta ccagccgaag cataaccatc gccaaactga
    14341 gtgaatgact gatttatctg agccttatat aataactgag caaccgtatg attcacatca
    14401 atcagctcca cttcaggaat ttgaacttca gattgttgcc ctaaagagac aatttttttt
    14461 gcgctggggc ctgatgaagg aatcgcagag catcctacaa ttaaacttcc acacaataat
    14521 aatactgcgt gacgaatata aaatttcact ttaaacacaa gccaaatcct aatataatta
    14581 taaatggcct aattatagca cttaatcgaa ataaatttat gagtacgtag agtataatta
    14641 gtattcttct ttccaacttc cttatactta tatacttata gattctaaaa tcatgaaaag
    14701 aattctttgc attacaggta ccagagccga cttcggcaag ctaaaacctt tattagccta
    14761 tattgaaaat cacccagacc ttgaattgca tttgattgta actggtatgc atatgatgaa
    14821 aacatatggc agaacctaca aggaagtaac tcgagaaaac tatcaacata catatctgtt
    14881 ttcaaatcaa atccaaggtg aaccaatggg tgccgtttta ggcaatacca ttacgtttat
    14941 ctctcgtcta tctgatgaaa ttgaacctga tatggtcatg attcacggcg accgtttaga
    15001 agcactagca ggcgcagctg taggtgcatt aagcagccgt ttagtttgcc atatcgaagg
    15061 tggtgaacta tctggtacag tagatgactc cattcgtcat tctattagta aactttctca
    15121 tatccacttg gtagcaaatg aacaagctgt cactcgccta gtgcaaatgg gagaaaaaag
    15181 aaagcatatt cacatcatcg gctcccccga tttagatgtt atggcctctt ccaccctccc
    15241 atccttagaa gaagtcaaag aatattacgg tttaccatac gaaaattatg gtatttctat
    15301 gtttcacccc gtgactacag aagcacattt aatgccacaa tatgcggccc aatatttcaa
    15361 agcattagaa ttaagtggcc aaaatatcat tagcatctac cctaataatg acactggcac
    15421 tgaaagtatt ctgcaagaat tattaaaata tcaatctgat aaatttattg catttccttc
    15481 cattcgattt gaatattttt tagtcttatt gaaacatgct aaatttatgg tcggcaactc
    15541 aagtgcaggt attagagaag ctcctctcta cggtgtccct tcaattgatg ttggtacacg
    15601 ccaaagtaac cgccatatgg gaaaatctat tattcataca gattatgaaa ctaaaaatat
    15661 ctttgatgcg attcaacaag catgcagttt aggcaaattt gaagcagatg atacctttaa
    15721 tggcggagat actcgcacca gcacagaaag atttgctgaa gtaatcaaca atcctgaaac
    15781 gtggaatgtt tctgctcaaa aacgttttat cgatttgaat ctttaaatta tggaaaaaca
    15841 aaatattgcg gttatacttg cgcgccaaaa ctccaaagga ttgccattaa aaaatctccg
    15901 gaaaatgaat ggcatatcat tacttggtca tacaattaat gctgctatat catcaaagtg
    15961 ttttgaccgc ataattgttt cgactgatgg cgggttaatt gcagaagaag ctaaaaattt
    16021 cggtgtcgaa gtcgtcctac gccctgcaga gctggcctcc gatacagcca gctctatttc
    16081 aggtgtaata catgctttag aaacaattgg cagtaattcc ggcacagtaa ccctattaca
    16141 accaaccagt ccattacgca caggggctca tattcgtgaa gctttttctc tatttgatga
    16201 gaaaataaaa ggatccgttg tctctgcatg cccaatggag catcatccac taaaaaccct
    16261 gcttcaaatc aataatggcg aatatgcccc catgcgccat ctaagcgatt tggagcagcc
    16321 tcgccaacaa ttacctcagg catttaggcc taatggtgca atttacatta atgatactgc
    16381 ttcactaatt gcaaataatt gtttttttat cgccccaacc aaactttata ttatgtctca
    16441 tcaagactct atcgatattg atactgagct tgatttacaa caggcagaaa acattcttaa
    16501 tcacaaggaa agctaaatgc aaaacaacaa cgaatttaaa attggtaatc gttcagtagg
    16561 ttacaaccac gaaccattga ttatctgtga aatcggcatc aatcatgaag gctctttaaa
    16621 aacagctttt gaaatggttg atgctgccta taatgcaggc gctgaagttg ttaaacatca
    16681 aacacacatc gttgaagacg aaatgtctga tgaggccaaa caagtcattc caggcaatgc
    16741 agatgtctct atttatgaaa ttatggaacg ttgcgccctg aatgaagaag atgagattaa
    16801 attaaaagaa tacgtagaga gtaagggtat gatttttatc agtactcctt tctctcgtgc
    16861 agctgcttta cgattacaac gtatggatat tccagcatat aaaatcggct ctggcgaatg
    16921 taataactac ccattaatta aactggtggc ctcttttggt aagcctatta ttctctctac
    16981 cggcatgaat tctattgaaa gcatcaaaaa gtcggtagaa attattcgag aagcaggggt
    17041 accttatgct ttgcttcact gtaccaacat ctacccaacc ccttacgaag atgttcgatt
    17101 gggtggtatg aacgatttat ctgaagcctt tccagacgca atcattggcc tgtctgacca
    17161 taccttagat aactatgctt gcttaggagc agtagcttta ggcggttcga ttttagagcg
    17221 tcactttact gaccgcatgg atcgcccagg tccggatatt gtatgctcta tgaatccgga
    17281 tacttttaaa gagctcaagc aaggcgctca tgctttaaaa ttggcacgcg gcggcaaaaa
    17341 agacacgatt atcgcgggag aaaagccaac taaagatttc gcctttgcat ctgtcgtagc
    17401 agataaagac attaaaaaag gagaactgtt gtccggagat aacctatggg ttaaacgccc
    17461 aggcaatgga gacttcagcg tcaacgaata tgaaacatta tttggtaagg tcgctgcttg
    17521 caatattcgc aaaggtgctc aaatcaaaaa aactgatatt agttaataat aaaatagatt
    17581 aagctattct taaattcaga atattgctta tctatattaa aaatttctaa tttttaaggt
    17641 tctgattgaa atcagaacct tatttcaact attacttttt actcataatc gaattatata
    17701 ctttaggact ttataatatg gctgttatta tatttgttaa cggaattcgg gctgtaaatg
    17761 gccttgttaa atcatctatc aatactgcaa acgcttttgc tgaagaagga ctggatgttc
    17821 atttaattaa ttttgttggc aatattactg gagcagagca tttatccccc ccattccact
    17881 tacatcccaa tgtcaaaacc tccagcatca tagatttatt taatgacatt ccagaaaatg
    17941 ttagctgccg aaatattcct ttttattcta tccatcaaca attcttcaaa gccgaataca
    18001 gtgcccacta taagcatgtt ttgatgaaaa ttgaatcttt attatctgaa gaagatagca
    18061 ttatcttcac tcatcctctt caactggaaa tgtatcgttt agcgaataat aatattaagt
    18121 caaaagccaa gctaattgta caaattcatg gtaactatat ggaagaaatc cataactatg
    18181 aaattttggc acgaaatatc gattatgttg attatcttca aacggtatct gatgaaatgc
    18241 tggaagaaat gcattcccat ttcaaaatca aaaaagacaa attagttttt attccaaaca
    18301 tcacttatcc catttcatta gaaaaaaaag aagctgattt ctttattaag gataatgaag
    18361 acattgataa tgctcagaaa tttaaacgta tctctattgt tggcagtatt cagccaagaa
    18421 aaaaccaatt ggatgccatt aaaatcatca ataaaattaa aaatgaaaat tacattttac
    18481 agatatatgg caaatctatt aataaagatt actttgaatt aattaaaaaa tatattaaag
    18541 acaataagtt acaaaaccgt atcttattca aaggtgaatc ttccgagcag gaaatttatg
    18601 agaatacaga tatcctaatc atgacatctc aaagcgaagg ctttggttat atatttctag
    18661 agggtatggt gtacgatatc cctatccttg cctataattt taaatatgga gcgaatgatt
    18721 ttagcaatta taatgaaaac gcttcagttt ttaaaactgg tgatatttct ggaatggcaa
    18781 aaaaaataat tgagctatta aataacccag aaaaatataa agaattagtt caatataatc
    18841 acaatcgctt cttaaaagaa tatgcaaaag atgtggttat ggctaaatat ttcactattc
    18901 ttccgcgcag ctttaataac gtatcattat cgtctgcttt cagccgaaaa gaattggacg
    18961 aattccaaaa tattactttt tctattgaag attctaatga tttagctcat atttggaatt
    19021 tcgagctaac caatcctgca caaaatatga atttttttgc tttagttggc aagcgaaaat
    19081 ttccaatgga tgctcatatc caaggaacac agtgtacgat taagatagct cataaaaaga
    19141 cagggaattt attgtcgctt ttactaaaaa aacgaaatca gttgaattta tcaaggggat
    19201 ataccttaat tgcagaagat aatagctatg aaaaatatat tggagcaata tctaataaag
    19261 gtaactttga aattattgca aataaaaaga actcattagt tactataaac aaaagtacct
    19321 tagagttgca tgagattccc catgaactac atcagaataa attactgatt gctttaccca
    19381 acatgcaaac gcctctaaaa attactgatg ataatttaat acctatccaa gcctccataa
    19441 aattagaaaa gattggaaat acttattacc catgtttctt gccatctggc atatttaata
    19501 atatctgctt agattacggt gaagaatcca aaattattaa ttttagtaaa tattcttata
    19561 aatatatcta tgactcaatt cgtcatattg agcaacatac agatatatcg gatattatcg
    19621 tttgcaatgt ttattcttgg gaacttattc gtgcctcagt tattgagagc cttatggaat
    19681 ttaccggaaa atgggaaaaa cactttcaga cttctcctaa aattgattat cgatttgatc
    19741 atgaaggtaa gcgttcgatg gatgatgtct tttcagaaga aacatttatt atggaatttc
    19801 cgcgtaaaaa tggtatagat aagaaaacag cagccttcca aaatatacca aacagtattg
    19861 taatggagta tccgcagacc aatggttaca gtatgcgcag tcattcactg aaaagtaatg
    19921 tagttgcggc aaaacatttt cttgaaaaat taaataaaat taaggtagat attaaattta
    19981 aaaagcatga ccttgcaaac atcaaaaaaa tgaatcgaat tatttatgag catttaggca
    20041 ttaacataaa tatcgaagca tttctaaaac cacgattaga aaaatttaag cgtgaagaaa
    20101 aatattttca tgatttcttc aaaagaaata attttaaaga ggtaattttt ccaagcactt
    20161 attggaatcc aggtattatt tgtgctgcac ataaacaagg tattaaggta tctgatattc
    20221 aatatgctgc cattactcct tatcatcctg cgtattttaa atcaccaaaa tcacattacg
    20281 ttgctgataa attgttctta tggtctgaat attggaatca tgagctttta ccaaatccaa
    20341 cacgagagat tggttctggt gccgcatatt ggtatgcatt agatgatgtg agattttcag
    20401 aaaaactgaa ttatgactat atctttctat ctcaaagtag gatttcttcg cgcttgctta
    20461 gttttgcaat tgagtttgca ttaaaaaatc ctcaactaca gcttttattt tctaagcatc
    20521 cagatgaaaa tatagattta aagaacagaa ttattcctga taatcttata atctccacgg
    20581 aatcttctat acaaggcatc aatgaatctc gcgttgctgt aggtgtttat tcaactagct
    20641 tatttgaggc attagcatgc ggcaaacaaa cttttgttgt taaatatccg ggatatgaaa
    20701 ttatgtcaaa tgaaatagat tcagggttat tctttgcagt agaaacacct gaagaaatgc
    20761 ttgagaaaac aagcccgaat tgggtggctg tggcagatat tgaaaaccag ttttttggcc
    20821 aagaaaaata atttttaagt taatctttaa aagtgcaata tttttcttat tttaaatatt
    20881 gcactttatt cttttaatca taggaactca catgtattct gaacagggaa ttaataatac
    20941 tattaacata tctactacat ctttaaccaa tgccacgcaa ctaactgtga tcgggaataa
    21001 taactctgta tatataggta ataattgtaa aatcgtcagc tccaatatta gattaaaagg
    21061 aaataatata accttattta tagctgatga tgtagaaatc atgggactag tatgttccct
    21121 tcattcggac tgctctttac aaattcaagc caagaccact atgggcaatg gagaaattac
    21181 cattgctgaa aaaggtaaga ttagtattgg caaagattgt atgctggcac atggatatga
    21241 aattagaaat actgatatgc atccaattta ttctttagaa aatggcgaac gaataaacca
    21301 tggtaaagac gttattatcg gtaatcacgt gtggcttggc cgcaatgtta ctatattaaa
    21361 aggtgtatgt attccaaata atgtagttgt aggttctcat actgtgctat ataaaagttt
    21421 taaagaacct aattgtgtaa tagcaggttc acctgcaaaa atagtaaaag aaaatattgt
    21481 atggggaaga aaaatgtatc attcaactat gtatgatgac cctactttaa atgaatttta
    21541 taaataa
//
LOCUS       DAWHXS010000027        20898 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWHXS010000027 DAWHXS010000000
VERSION     DAWHXS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.027"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            236..1189
                     /locus_tag="WIE22_000764"
     CDS             236..1189
                     /locus_tag="WIE22_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ4126887.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
     gene            complement(1431..2678)
                     /gene="fabF"
                     /locus_tag="WIE22_000765"
     CDS             complement(1431..2678)
                     /gene="fabF"
                     /locus_tag="WIE22_000765"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ4126888.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(2834..3070)
                     /gene="acpP"
                     /locus_tag="WIE22_000766"
     CDS             complement(2834..3070)
                     /gene="acpP"
                     /locus_tag="WIE22_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ4126889.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            3302..4327
                     /locus_tag="WIE22_000767"
     CDS             3302..4327
                     /locus_tag="WIE22_000767"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ4126890.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVYEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     RSAQNAV"
     gene            4634..4957
                     /locus_tag="WIE22_000768"
     CDS             4634..4957
                     /locus_tag="WIE22_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126891.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIALYKEDSYLVNMV
                     KRIFK"
     gene            5044..5325
                     /locus_tag="WIE22_000769"
                     /pseudo
     CDS             5044..5325
                     /locus_tag="WIE22_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5378..5851
                     /locus_tag="WIE22_000770"
     CDS             5378..5851
                     /locus_tag="WIE22_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ4126892.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            5865..6200
                     /locus_tag="WIE22_000771"
     CDS             5865..6200
                     /locus_tag="WIE22_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126893.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKSAAY
                     AILKERGEV"
     gene            6398..9088
                     /gene="glnE"
                     /locus_tag="WIE22_000772"
     CDS             6398..9088
                     /gene="glnE"
                     /locus_tag="WIE22_000772"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ4126894.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRELRCYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYDYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLEKLAESGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQRLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPGEQTQSNGEWQWVWQEKPDEEGRQCRLKEHGFDAETIAAR
                     LDQIRNGHKYRRLSAHAQPRFDALVPLLVQAAAAQTNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACGGDTEAQMDTLRHFQHAQVFRLTVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWEDMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLANYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(9388..10080)
                     /locus_tag="WIE22_000773"
     CDS             complement(9388..10080)
                     /locus_tag="WIE22_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ4126895.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(10188..11381)
                     /locus_tag="WIE22_000774"
     CDS             complement(10188..11381)
                     /locus_tag="WIE22_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ4126896.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKRAQDIVNQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVT
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(11510..12247)
                     /gene="pxpA"
                     /locus_tag="WIE22_000775"
     CDS             complement(11510..12247)
                     /gene="pxpA"
                     /locus_tag="WIE22_000775"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ4126897.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIANIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPNAVVFAEKIRQELLAAGIKVSA"
     gene            complement(12234..13163)
                     /locus_tag="WIE22_000776"
     CDS             complement(12234..13163)
                     /locus_tag="WIE22_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ4126898.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGKLAQVRFGSK
                     VKFKIIGLKKATALRRKNQAYLNQIRRITHEAG"
     gene            complement(13160..13807)
                     /gene="pxpB"
                     /locus_tag="WIE22_000777"
     CDS             complement(13160..13807)
                     /gene="pxpB"
                     /locus_tag="WIE22_000777"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ4126899.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            13991..16198
                     /gene="uvrD"
                     /locus_tag="WIE22_000778"
     CDS             13991..16198
                     /gene="uvrD"
                     /locus_tag="WIE22_000778"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ4126900.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASQEQGVSLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMPLPEIIAGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPDNISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            16555..17094
                     /locus_tag="WIE22_000779"
     CDS             16555..17094
                     /locus_tag="WIE22_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ4126901.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYQEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(17284..17499)
                     /locus_tag="WIE22_000780"
     CDS             complement(17284..17499)
                     /locus_tag="WIE22_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126902.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(17507..17710)
                     /locus_tag="WIE22_000781"
     CDS             complement(17507..17710)
                     /locus_tag="WIE22_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126903.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(17623..17982)
                     /locus_tag="WIE22_000782"
     CDS             complement(17623..17982)
                     /locus_tag="WIE22_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ4126904.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            18035..18382
                     /locus_tag="WIE22_000783"
     CDS             18035..18382
                     /locus_tag="WIE22_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4126905.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVLVPF"
     gene            18610..19230
                     /locus_tag="WIE22_000784"
     CDS             18610..19230
                     /locus_tag="WIE22_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ4126906.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGILYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            19253..20704
                     /locus_tag="WIE22_000785"
     CDS             19253..20704
                     /locus_tag="WIE22_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ4126907.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         5022 a         5387 c         5412 g         5077 t
ORIGIN      
        1 aaaccggatg attcgggcta aataattcct gattctttgg atatagccaa cggtatgcct
       61 gccgatgccg tcagaaaggg cttgatgggc gtttgcggtt cgggttaata tttgtgctgt
      121 atctgttaat tccggttagt aaaatggggc ggggacgatg agtggccgtc ggtcggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccggcttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggcgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgccggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggca tttgcctgca ttgtcggcgg cgtggcttcg gttcacggac accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacaggattg agcgcgaaat ttttcccagg
     1081 caaccccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gccccgcctt
     1261 agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat
     1321 ttgtactgtc tgcggctcgc cgccttgtcc tgatttttgt taatcactat aaaaatgccg
     1381 tctgaaacgg tttcagacgg catttcgatg tcggcggcgg ctttgcggaa tcagcctttg
     1441 aagcgtttga agaccagcgt gccgttggtg ccgccgaagc cgaaggagtt ggaaatggca
     1501 acgtcgattt ccgcgtcgcg cgcttcgttg gcgcagtagt ccaaatcgca gccggcttca
     1561 acgtcttgtt caaaaatgtt gatggtcggc gggattttgc cgtcgtgtat cgccaaaatg
     1621 ctgtacacgg cctccacgcc gcccgccgcg ccgagcaggt ggccggtcat ggatttggtc
     1681 gagctgacga cggttttgta ggcgtgttcg ccgaacgcgc gtttgagggc tttggtttcg
     1741 ttggcatcgc ccaagggggt ggacgtgccg tgcgcgttga cgtaatccac gtcttcggga
     1801 ttgatgccgg catctttcag cgcgcgggta acggcaaggg cggggccttc ttcgttcggc
     1861 gcggtgatat ggtaagcatc ggaactcatg ccgaagccga cgatttcggc gtagattttc
     1921 gcgccgcgtt ttttggcgtg ttccaattct tccaacacca atatgcccgc gccttcgccg
     1981 ataacgaagc cgtcgcggcc tttgtcccac ggacgggaag cggtggcggg gtcgtcgttg
     2041 cgggtggaga gggctttcat cgcggcaaaa ccgcccacgc ccaaagtgct gattgcgcct
     2101 tccgcgccgc cggcaaccat tatgtccgcg tcgccgtatt tgatcagtcg ggcggaattg
     2161 ccgatggcgt gcgcgccggt ggtacaggcg gaaaccatcc cgtagctcgg gccgcggtag
     2221 cctttgagga tggtaacgtg tccggaaatc aggttgatca gcgaaccggg gataaagaaa
     2281 gggttgattt tgcgcgcgcc gccttcgatt acggctttgc cggtgacctc gatgctgggc
     2341 agtccgccga tgccggaacc gatgttcacg ccgatgcggt ctttgtcgag gttttccaca
     2401 tcgtccaagc ccgaatcggc gattgcctgc aatgcggcgg caatgccgta gtggatgaat
     2461 acgtccatcc ggcgcgcttc tttcgcgctg atgtattgtc cgatgtcgaa accgcgcact
     2521 tcgccggcga cacggctgtt gatgtcggat gcgtcaaagc gggtaatcgc gccgatgccg
     2581 cttttgccgg cgagcagggt gtcccaagcc tctgcgacag tgttgccgac aggggaaacc
     2641 tgacctaagc ctgtaatgac tactcttctc tgactcatga taacctcgct gttggttgtc
     2701 ggaatggggg catatgcggc tgtcgtgcag atgccgtctg taatttgcgg caggggttca
     2761 aacagtttgc catataaggg aaaagcctct attgcgcggt gcagcagagg ctgttgtgtc
     2821 gggcgacgac cggttagccg ttgtgggcat tgatgtagtc gatggccagt tggacggtgg
     2881 tgattttttc ggcatcttcg tcggggattt cgcagccgaa tgcttcttcc aaagccataa
     2941 ccagctccac ggtgtccaaa gaatccgcgc ccaagtcgtc ttggaaagaa gattcgtttt
     3001 tcacgtcggc ttcgtttacg cccagttgtt cagcaacaat ttttttaact tgttgttcga
     3061 tgtttgacat atcagtcgtt cctttatgcc ttgcggcagg ttgtttaagg gaaataaatc
     3121 ggtggtattg taccgacttt taatagagtt ttctatctaa tgactattat atcaatattt
     3181 gccgatttgt acatttttgg gtgcggcggg ttttgtcgtt caagtttgac ctgtgtgccg
     3241 tatgtttggc gggatttcgg ttaaaatggc ggcatttcca tctgaagcag aaagccctgt
     3301 catgtatcca cttgcccgcc gcatcctgtt tgcactcgat gccgaaaaag cccaccactt
     3361 cacgctcgac gcgctctaca cggtttataa attgggtctg attcccgtaa ccggcaaccg
     3421 taccaaacct gtaaaactga tgggtttgga tttgcccaac cctgtcgggc ttgccgccgg
     3481 actcgataaa aacggcgaat atatagacgc attgggcgcg ctcggctttg gtttcatcga
     3541 aatcggcacg gtaacgccca aaccgcagcc cggcaatccg cagccgcgtc tcttccgcgt
     3601 tcccgaacac caaggcatca tcaaccgcat gggcttcaac aaccacggca tcgatgccat
     3661 gattcaaaac atcgaaaaaa gtaaattcag cggcgtgttg ggcatcaaca tcggcaaaaa
     3721 cgcggttacg cccatcgaaa atgctgccga tgattattta atctgccttg aaaaagccta
     3781 cgcacacgca agttacatta ccgtcaatat ttcctcgccc aacactaaaa acctccgcgc
     3841 gctgcaaggt ggcgacgagt tgagcgcatt gcttgaggct ttgaaaaaca aacaggcaca
     3901 gcttgcctct gtacacggga aatacgtccc gctcgccgtc aaaatcgccc ccgatttgga
     3961 tgaagcacaa attgaagaca tcgcccacgt tgtcaaatcc gtcgaaatgg acggcatcat
     4021 cgctaccaac accaccatcg acaaatcaag cctcggcagc catccgcttg caggcgagca
     4081 gggcggtcta agcggtctgc ccgtttatga aaaaagtaat cgggtgttga agctgttggc
     4141 agaccacata gacggcaagc tgccgattat cggcgtaggc ggcattatgg aaggcaggga
     4201 cgcggcagag aaaatccgct tgggcgcgac cgccgtccaa gtgtacagcg gattgatata
     4261 caaaggtccg gcattggtca aagaatgttt gaaggctttg gcgcgatccg cccaaaatgc
     4321 cgtctgaacg cacgttttgc cgttcagacg gcattttcat ttcctttttc cgcctgacgc
     4381 cccttgaaaa tcccttacgc gccgccctgt ttgaaacaaa gcaaacgccc gccgcgaacc
     4441 ggacaatctt gatacgcagc caaagcaagt ccggcggtaa gttgttaaat atataagcaa
     4501 ttgatacaaa aaataatttt atactgtggc ggacggcttg ggaaaacgca aaatatgaac
     4561 ggcgttgaaa ttcatgatgt ggaaaataag gatacggcgg ataatcaaaa gattttatat
     4621 ttaaggattt gatatgttta aggacgaact aaatgaattt atccgattga tatccgatcc
     4681 ggaaagtgaa ttggatgaat ggtatttgag tgattttaaa gatgaacata tatgggaaat
     4741 gcaaagttac gaagcatttt catgtttgcg ggaagcagtc ccctatttgt ttgcttatcc
     4801 ccgatacggg tatgaactgt tggagataat ttccgcgtta aaagaaacat ctgatacgac
     4861 agaattattt tatgagccgg gtatcgtgcc tttgctgatt gctttatata aagaagatag
     4921 ttatctggta aatatggtca aacggatatt caaatgatga aaggggcgcg ggcgtttgct
     4981 tggcggaagg caggtaactc aatttgaagg acgcggaagc cgatacgtta aaaaggatga
     5041 aaaatggaaa acgggcaacg gaataaccgg tttcctttgg aaaaacgaat tttttatctg
     5101 gagcattcgg ggcggtatct gatgatttgc gccttatcgg attattccca aaacaaacat
     5161 actgtcgtta tggcaaattt catctatccg gatgaaaaaa cggattagcg gaatttggat
     5221 gatttattca atgagttggt tttagaggaa ttgcaggctt catttatgga ttggcatccg
     5281 actgttgaag aggcaatcag ccgtcatttg gaagactttt cgtaacaggc gtcaatcgga
     5341 aggggcggaa tgacggggca ggcaatcgga gtaaaaaatg aaccttgatt taaccgcgca
     5401 aaaagtccgt ctttcttgga aggatattct gtgggggtat gggaataaat acttgggttg
     5461 ggctgatgtg gcagcttatg cccgaaaaat gacgctttca gatcatgatg aacgtgtgtt
     5521 caaactatct ttaatcaaca aatccaatat tcttgaatta aagcctgttc tggaagattt
     5581 ggcttcggaa atgagggatt attcccctaa aaattggctg tacgtcctct taagcgatgt
     5641 attccataga aaagaagaat ttgaggatcc tttgggggaa gttgaaaaaa tttatgcaga
     5701 ttttgattat ccggaagaaa tagaatcatt tgtcaggtat atgccgccca aagacggtta
     5761 tattccttct gcccacacct atgaagaaaa tattgcccgg ttatattctc actgggaaca
     5821 ctatttgaac aacggcggag ggcagggtta aaaccggcaa tccgatgccg tctgaagcat
     5881 tatccggcct tcagacggca ttttgttttc cgacagttta taaactgtcg ttgtttcttg
     5941 acagaaacaa cgaccttatt tatttgaaac gattggagga catgattatg ggtttttgga
     6001 atggtgtggc aaaagcagca aaagcagtgg gagagggaat gattgaagcc ggcaatgagc
     6061 ataaggcgtt gaaaatggaa tatgcggaga aatcaagtga ggagctgcat gaaatcgtca
     6121 agagtgatgg tttttttaaa aattccacac gggagaaaag tgcggcttat gctattttaa
     6181 aagagcgtgg cgaggtgtga acaggaaacg gcggcatttg ccgctgtttt ttatcggcag
     6241 gcatccgtct agatgtcggg gcaaggtttc agacggcatc gaaggttgct atgatatagt
     6301 ggcttgactt taaaccggta cggcatcccc tcgccctgtc ctgatttgaa gtgaatccgc
     6361 catcatcttc ctgccatttt ttcaaacgga atccgaaatg tccgacaacc gcctcgacac
     6421 cgcccgccgc cattccctct tcctcgcccg ccagctcgac aacggcaaac tcaagcccga
     6481 aattttcctg cctatgctgg acaaggtttt gaccgaagcg gatttccaag cctttgccga
     6541 ctgggacaaa atccgtgcgg aagaaaacga ggaagaattg gcgcggcagt tgcgcgagtt
     6601 gcgctgttat gtggtgtcgc agattatcgt gcgcgatata aaccgcatca gcgatttgaa
     6661 cgaagtgacc cgcacgatta cgctgtttgc cgattttgcc gtcaataccg cgctggactt
     6721 tgcctacgac tattatcggg acatgtacgg cacgccgatc gggcgttata ccaaatcgcc
     6781 gcagcatttg agcgtggtgg cgatgggtaa ggcgggcggt tatgagttga atgtgtcttc
     6841 cgacatcgat ttgattttcg tctatcccga atcgggcgac accgacggca ggcgcgaacg
     6901 gggcaatcag gaatttttca ccaaagtcgg gcagaaactg attgcgttgt tgaacgacat
     6961 taccgccgac gggcaggtgt tccgcgtcga tatgcggttg cggccggatg gcgattcggg
     7021 cgcgttggta ttgagcgaaa ccgcgctgga gcaatatttg attacgcagg ggcgcgaatg
     7081 ggaacgctac gcgtggtgca aaggccgcgt ggttacgccg tatccgaacg gaatcaaatc
     7141 gctggtgcgc cccttcgtgt tccgcaaata tctggattac ggcgcgtatg aggcgatgcg
     7201 taagctgcac cgccaaatcc gcagcgaagt cggcaaaaaa gggatggcgg acaatatcaa
     7261 actcggcgcg ggcggcatcc gcgaagtcga atttatcgcc cagatttttc agatgatacg
     7321 cggcgggcaa atgcgcgcgc tgcaactgaa aggcacgcag gaaacgctgg agaaacttgc
     7381 cgaatcgggc attatgccgt ctgaaaacgt cgaaaccctg ctcgccgcct accgcttttt
     7441 gcgcgatgtc gaacatcgtt tgcaatattg ggacgaccgg caaacccaaa ccctgccggc
     7501 ttcgcccgaa cagcggcaac ggctcgccga aagtatggga tttgatgatt acgccgattt
     7561 tttgaatgct ttaaatcaac atcggaacca agtcaatcgg ttgtttaacg aaattttgag
     7621 cgaacccgga gaacaaacgc aaagcaacgg cgaatggcag tgggtttggc aggaaaaacc
     7681 cgacgaagaa gggcggcaat gccgtctgaa agaacacggg ttcgatgccg aaaccatcgc
     7741 cgcgcggctc gaccaaatcc gcaacgggca taaataccgc cgcctttccg cccacgccca
     7801 gccgcgtttc gacgcgcttg tgccgttgct ggtacaggcg gcggcagcgc aaaccaaccc
     7861 gaccgataca ttgatgcggc tgttggattt cctcgaaaat atcagccgcc gttccgccta
     7921 tctcgccttc ctcaacgagc atccgcaaac cctggcgcaa ctcgctcaga ttatgagcca
     7981 aagctcatgg gtggcggcgt atctgaacaa atatccgatt ttgttggacg aactcatcag
     8041 tgcgcaatta ttagatacgg cgtttgattg ggacaaactc gccgccgaac tttcagacgg
     8101 catcaaagcc tgcggcggcg acaccgaagc gcaaatggac accctgcgcc atttccaaca
     8161 cgcccaagtc ttccgcctca ccgtccaaga cctcgccgga ttgtggacgg tagaatccct
     8221 ctccgaccaa ctctccgccc ttgccgacac cgtcattgcc gccgcccttt cgtgcgcgtg
     8281 ggaggatatg cccaaaaaac accgcgacac accgcaattc gccatcgtcg gctacggcaa
     8341 actcggcggc aaagaactcg gctacgcctc cgacctcgac ttggtctatc tttatgacga
     8401 cccgcatccc gatgcaggcg acgtgtacag ccgccttgcc cgccgcctga ccaactggct
     8461 ttccgccgcc accggcgcag gcagcctcta cgaaaccgac ctgcgcctgc gccctaatgg
     8521 cgacgccggt ttcctcgccc acagcatcgc cgcctttgaa aaataccagc gcgaaaacgc
     8581 ctggacgtgg gaacaccaat cccttacccg cgcccgcttc atctgcggca cacccgaaat
     8641 tcagacggcc ttcgaccgca tccgcaccga aatcctcacc gccgaacgcg accaaaccgc
     8701 cttgtcaggc gaaatcatcg aaatgcgcga aaaaatgttc cccacccatc cgcctgccga
     8761 cagcaatgtc aaatacgcac gcggcggcgt ggtcgacgtc gaatttatcg tccaatacct
     8821 catccttgcc catgcccgaa aatatccgca acttctcgcc aactacggca acatcgccct
     8881 cttgaatatc gccgccgact gcagccttat cgacaaaaac ctcgccgaac aaagccgcac
     8941 cgcctaccgc ttctaccgcc ggcagcaaca caacaccaaa ctgcgcgacg cagaaaaaac
     9001 cgaagtaagc ggcgaattgc tggcacatta cggcaatgtc aggaaattgt ggcgggaagt
     9061 gttcggcgaa gaagcggcaa ccgcctgaac aaaaaatgcc gtctgaagcc tgacaatctg
     9121 ggtttcagac ggtattttcg taccgtgccg ttttaaggtt gcggcagagc taaagcgatt
     9181 tatcgggaat ggctgaaacc caaaaaccgg attcctcttt cagttacagc agcttgatcc
     9241 ctttccctta tccaacgggg gaaggctagg atagggtggc ttgcaaatat acagaacaag
     9301 ggacaagagc caccctctct ccaaccctct ccctccgtac gggagggggt ggattctcgc
     9361 gggcgaagcc cacgctacgg ttagccttta ccccagcaca aacaattccc gcccgtgcgc
     9421 cttcagccaa cttttagcat tgtcggtatg cggcgtcagc gtgttcacca aatgccaaaa
     9481 gcgcggactg tggtcggggt ggcggaggtg gcagagttcg tggatgcaga catagtcggc
     9541 gacgtattcg ggcgtgccga tcagccgcca gttgaggcgg atgccggtgt gcgggcggca
     9601 tacgccccaa aaggttttgg cgttgctcag gtctgtggcg gtgggcgtca gtcctgtttc
     9661 ggctgcgtgt ttttcaaggc ggggcagcag gtattcgcgg gcgcgttcgt tcaacaggcg
     9721 gcgcaggtgg tcgatttgtg cggcggtttc ttttcgggga agcaggattt cagacgacgt
     9781 gatacggata tggctttggc tgtgggtatc cagcttggtc tttattcccc gataccaaat
     9841 ccactcgggt aagtttgggt gggaaacagg atgcacgggc gttttggcaa gcgtgttccg
     9901 caaaatcgtt tcgtttgccg ccagccagtt tgctaacgcg tggtcttgaa aaaagggtgg
     9961 gacgttgatg ctgaccgtct gcatattgac ggggcgcaga atcagatttt tcttggcact
    10021 gcgtttgagt tcgatttcga tgcacaaacc gtcggaaaga gtataggtga agcgtttcat
    10081 agttgtgaat aggtttcaga ccggatacat cgtctgaaac aggaattttc catatcaggc
    10141 ggcaaacttc ggataatata caaaatcaaa catctgcgct acaaggttca gccgaacaag
    10201 ccgccgatat atttgctgat ggtgatggcg ctgagtactg ccatcaaacc gaccacaatc
    10261 acgccggaaa cggtgagcca cagcgggtgt ttgtagtcgc cgacaatttt ggttttgtag
    10321 gcggcaatca gaatcagacc gagggaaatc ggtaaaatca ggccgtttaa tgcgcctacg
    10381 aacaccagca cctgcgccgg tttgccgatg gtggaaaata cggcggtgga cacggcgata
    10441 aaggcaataa tccatttgtt tttattgcgt tcgatagacg ggctgagacc ggagaagaac
    10501 gacaccgaag tataagccgc accaatcacc gaagtaatcg aagccgccca aatcaccacg
    10561 ccgaaaatca gcaggccgat gtatcccgcc gcatattcaa acggtgtgga agcagggttg
    10621 tcgggattga gctgtacgcc ttggctgacc acgcccaaaa ccgccaaaaa caatacaatc
    10681 cgcataatcg aggcaatcag gatcgcccgc accgagcttt ggctcacttc cggcaacgcc
    10741 gatttgcctt tgatacctgc gtccagcaga cggtgcgcac cggcgaaggt gatgtagccg
    10801 ccgaccgtgc cgcccaccag tgtaacaatc gccattgcat cgagtttttc cggcataaag
    10861 gtatgcacgg cggcatctgc cagcggcgga ttcgcctgcc atgtcacata aaccgtcagc
    10921 gcaatcatta cgaaacccat cacttgggcg aatttgtcca tcactttgcc tgcttcttta
    10981 aacagaaaca caccgatggc aatcacgccg ctgatcacgg caccggtttc cggcgacagt
    11041 ccggtcagca ggttcagacc caagcctgcg ccgccgacgt tgccgatatt gaacgccaaa
    11101 ccgcccatca caatcagcac agccaagaaa tagcctgcgc cgggcaagac ctgattgaca
    11161 atatcctgcg cccgtttttc ggaaacggcg acaatccgcc aaatattgag ctgcgccccg
    11221 atgtcgagca gaatcgagag cagaatcaca aagccgaaac ttgccgccag tgcttgggtg
    11281 aaggtggcgg tttgggtcag aaagcccggg ccgatagcgg aagtcgccat caggaatgca
    11341 gcgccgatta aggcatttct gcggtttttt tgatcagaca taatcgctta tcctctataa
    11401 aattggttgt tgctgtgttt gggcgaaacc tgcggtttta gctacgcaga aactcgcttt
    11461 gctcgttttg gcgaaacctg cggttttcag acggcctatg aactgttttt caagcagaaa
    11521 ctttgatgcc tgccgccagt agttcctgcc ggattttttc ggcaaacacc acggcgttcg
    11581 gcccgtctcc gtgcagacag atgctgtcgg cttgcacggc aaccaggctg ccgtccactg
    11641 ctttgacctg cccgtcccgc accatctgca atacttgggc gatggcttct tcgtcgctgt
    11701 ccacctgcgc atcggggcgg ctgcggggaa ccagcgtacc gtcgggcata tagcggcggt
    11761 cggcgaatac ttcggaaatc acacccaagc ctgcggcttt tccggcttcc aagagcaggc
    11821 tgccggaaag tgccatcaat ttcaatttcg ggtcgaaatc cgccacaatt cgggcaacgg
    11881 tatccgccag cgcacggttt ttcgccgctt gattgtacat tgcgccgtgc ggtttgacat
    11941 aagccatttc caaaccctga tcacggcaca aggcctgcaa tgcgcccaac tggtaattca
    12001 gacacgcccg caaatcggct tcggacagat tcatttcggt acggccgaag ttttcccgat
    12061 cgggatagcc ggggtgtgct ccgatgcgca cgccgttttg ttgggcatac gccaatgccg
    12121 cccgaatatt ggcaatgctg ccggcgtgtt gggcgcaggc gatgttggcc gaagtaatca
    12181 gctgcaacaa ggcttcgtcg ctgccgcagc cttcggcgag atcggcgttt aaatcaacct
    12241 gcttcatggg tgattctccg tatttggttc agataggctt ggtttttgcg ccgcagggcg
    12301 gtggcttttt tcaagccgat tattttgaat ttgactttgc tgccgaagcg cacctgtgcc
    12361 agcttgccca aatcggcggc ggcaacggta gcgattttcg gataaccgcc ggtggtttgc
    12421 gcatcggcca gcaggataat cggtttgccg ccgggcggca cctgcacggt tcctgcctga
    12481 acagcgtggg acagcatttc caaaggttgc gacagggtca gcggctgtcc gtcgaagcgg
    12541 tagcccatgc ggttgctatc gctttgcagc gtccacgttt cccgttccag attcagacgc
    12601 cctttttcac tgaaagcggc atattccgac gaaggaacaa ggtggacggt atcggtaaac
    12661 ggtatcgggg caatgccgac tttggacaat tcctgcgcac ctttgccgat ggggagataa
    12721 tcgccttttt gcagcattct gccctgatgg ccgccgaaac cggctttcag gtcggtactt
    12781 ctcgaaccca tcacttccgg cacatcaaat ccgcccgcca cgcacacata gccgtacatg
    12841 ccctgcacgg cacgcaccag tttcaaggtc tgccctttgc gggcggtata acgccaatac
    12901 gaatagaccg gttcgccgtc caattccgcc tgatacacgg caccggtgag acaaaacggc
    12961 gtatcccgtt caaacaccag cattatcccg cccaaagcga tttcgattgc ggccgtgcct
    13021 tcgtcgttgc ccaataaaat attacccgcc gccaaagcaa ccgtgtccat cgcaccggca
    13081 tgaccgatgc cgtaacgccg gtgtccgtag cgtccggtat cctgaatatg cgccggtgcc
    13141 tgcactgccg aaacgtgaat catggctcaa tcctttctgc aacaaagcgg acttggtcac
    13201 ccgccgccag cagggtcggc ggattcaaat cggctcggaa caagggtaat tcggttctgc
    13261 cgataatctg ccagccgccg ggcgaagcga acggatacac accggtctga ctgccgccga
    13321 taccgaccga accggcagga acggacgttc tcggcacggc acggcggggc gtgtgcaatg
    13381 cttcgggcaa gccgcccaga taagggaaac caggctggaa gcccatcata aatacggtat
    13441 aagtttgcgc cgtatggcgg cggacgattt cggaaataac cgtctgatgg aaagcagcga
    13501 cttccgccaa atccgggccg tattcgccgc cgtagcagac gggaatttcc accagtttgc
    13561 cctgatggtc tgtaacggcg gtgtgttccc acacatattg caattcatcg gcaagcgtcg
    13621 ccaaatcggt atcgaaacgg gtgaacacgg tcagattgtt catgccgacc accacttcct
    13681 caatcctgtc gtgctgcccg agcgcagcgg caaacgccca caacttttgc tgtttgccca
    13741 gttcggaagg tgcattcagt cggcagacca aagcggattc gctgattggt gtgatctcta
    13801 ttctcatttg ttgttcattt tggttatgtt ttaatgaatc tatatgcagg ggcggcggtt
    13861 tgtcaatatc ttctgtgctg catcatcaaa ccgtcgattg gaaaagtgct gccctgccgc
    13921 tgcacttttt cagacgacct taaaccgttt ctattaaaat agcgcattcc acttttcaga
    13981 cggcatctct atgtttcccg accaatccgc ccccaacctg ctgcaaggct tgaaccctga
    14041 acaactttcc gccgtaacct ggccgccgca atccgccctt gtgctggcag gcgcgggcag
    14101 cggcaaaacg cgcgtgctga ccacgcgcat cgcatggctg ttgcaaaccg gtcaggcaag
    14161 cgtgcacagc attatggcgg taacgtttac caacaaagcc gccaaagaaa tgcaaacccg
    14221 tttgggcgcg atgattccca tcaatgtccg cgccatgtgg ctcggcacgt tccacggcct
    14281 ctgccaccgc tttttgcgcc tgcaccaccg cgacgccggt ctgccgtctt cctttcaaat
    14341 cctcgacggc ggcgaccagc tttccctcat caaacgcctg ctcaaaagcc tcaacatcgc
    14401 cgaagaaatc atcgcgccgc gttcgctgca aggctttatc aacgcgcaaa aagaatccgg
    14461 tttgcgcgct tccgtcttga gtgcgcccga tccgcacaca agccgcatga ttgaatgcta
    14521 cgccgaatac gacaaaatct gccaacgcga aggcgtggtc gattttgccg aactcatgct
    14581 ccgcagctac gaaatgctgc aaagcaacga aatcctgcgc cagcattacc aaaaccgctt
    14641 caaccacatt ctcgtcgacg agttccaaga caccaacaaa ctgcaatacg cctggctcaa
    14701 actcatggcg ggcggaaacg cagcggtatt tgccgtcggc gacgacgacc aaagcattta
    14761 ccgtttccgt ggcgcaaacg tcggcaacat gaccgcgctg atggaagaat tccacatcga
    14821 cgcgcccgtc aaactcgaac aaaactaccg ctccgtcggc aacatccttg ccgccgccaa
    14881 tgccgtgatt gaaaacaacg acgaacgact cggcaaaaac ctgcgcaccg acgccgaagc
    14941 aggcgacaaa atccgctact actccgcctt taccgacctc gaagaagccc ggttcatctt
    15001 ggacgaaacc aaagccctcg aacgcgaagg ctgggatttg gacgaaatcg ccgtcctcta
    15061 ccgtagcaac gcccaatccc gcgttatcga acaaagcctg ttccgcagcg gcatccccta
    15121 caaaatctac ggcggcttgc gcttttacga acgccaagaa atcaaacacg cgcttgccta
    15181 tctgcgcctc gccgtcaatc ccgacgacga caacgccctc ttgcgcgtga ttaacttccc
    15241 accgcgcggc atcggcgcac gcaccgtcga aaaccttcag acggcatcgc aagagcaagg
    15301 cgtttcactt tggcaggcag cctgcaatgc cggcgcaaaa gccgccaaag tcgccgcctt
    15361 cgtccgcctc attgaagccc tgcgcaacca agtcggacaa atgcccctgc ccgaaatcat
    15421 cgccggcatc ctcaaagaca gtggcttgac cgaacactac cgcacccaaa aaggcgacaa
    15481 ccaagaccgc ctcgacaacc ttgacgaact cgtcaacgcc gccatcgagt tcaaacccga
    15541 agacagcaac tttgaaaccc tgcccgacaa catttcagac gaccccgcct tccccatcct
    15601 cgctttcttg agcaacgccg ccctcgaatc cggcgaaaac caagcaggcg caggcgaaaa
    15661 agccgtccaa ctgatgaccg tccacgccgc caaaggcttg gaattcaacg ccgtcttcct
    15721 caccggcatg gaagaaggcc gcttccccag cgaaatgagc cttgccgaac gcggcggcct
    15781 cgaagaagaa cgccgcctca tgtacgtcgc catcacccgc gcccgcaaac gcctctacat
    15841 caccatggcg caacaacgca tgctgcacgg acaaacccaa ttcggcatcg tctcccgctt
    15901 cgtcgaagag atcccacccg aagtattgca ctacctgtcc gtcaaaaaga ccgcctacga
    15961 cagctacggc agcccgcgcc aaaccgccgc atccaaagac aaaatcatcg acgactacaa
    16021 acagccccaa acctacgcag gtttccgtat cggacaaaac gtccgccacg ccaaatttgg
    16081 caccggcgtg attatcgatg ccgcagataa aggcgaatcc gcccgactga ccatcaattt
    16141 cggcaaacag ggcgtgaaag agttggacac caagtttgcg aaattggaag agatgtaaat
    16201 ttgaaatgca ggctggatac tcgtatccga caaaaatatt tgacgcgtct atcgtttccg
    16261 aaaaaccgct gttggaaaat gtcggattcg agaatccgac ctatggcaaa aaactgtagc
    16321 aaggacaagg caaaaggccg tctgaatccg gtaacaacaa attttgccga aacctccgcc
    16381 gtcattcccg cgcaggcggg aatccagacc tttcggcacg gaaacttatc ggataaaagg
    16441 tttctttaga ttccacgttc tagattcccg cctgcgcggg aatgacggac ggtaaaagcc
    16501 gggtatagat acctaaccct tgtttatagc tgaaaatcaa taaggaagaa cattatgtcc
    16561 caagttttta aagattttga tttgtcctcc gtatgggaat ccgacagttg ggcagatgaa
    16621 aactaccaag aagccccgtt tacccctgaa attttggctg ccgtagaaag tgaactgggc
    16681 tataaattgc cgcaaagttt tattgaattg atggcggtac aaaacggcgg aatatttgtc
    16741 aaaaactgtt ttccgaccac gcagagaaat tcgtgggcgg aaaatcatgt gcaaatttgc
    16801 gaggtatcgg gcatcggctt tgaaaaagaa gggagtttgt gcggcgaaac agggcaaaaa
    16861 ctttggctgg aagaatggga atatccgccc atcggcgtgt attttgccaa cgacccgtca
    16921 ggcggtcatg ccatgtttgc cttggactat cgggcgtgcg gcaaagacgg cgagccgaaa
    16981 gtggtgtttg tcgagcaaga atcggatttt gaaatcgtcg aacttgcccc cgattttgaa
    17041 acctttatcc gcagcttgcg gcatgaagat gagtttattg acgaggaaat ataaaacggc
    17101 ggttgaaaaa ccggaatcat caagggaaaa cgggcgaaat aacggataat cgcttgaatc
    17161 cgtaaggaaa acggtttggt ggaacgcgcc atccaagacc tttgcaaaga aaaactgtct
    17221 ccgacagcat tgacattatt aacagaactt atcaagtttg gagctatctc aaatataatt
    17281 cggttatcct tttgtatcca ttaaatcata tgcttcaatt aattgttgtt ctagctcttg
    17341 taccaatttt gaattgcgaa ttcctgacac aatctcaaat tcttctgcat ctatgcaaac
    17401 acctgcataa atttcaataa caagggaacg caataattga agctcttctc ttgttaaaga
    17461 aataataatg tcatcacctg tgtaattgat tatattcata ataattttat ttttgtttgt
    17521 caaagtaagt tttgcctaag gttggtctaa atgcagttcc accatctttt gaatttgggt
    17581 ctctgattac aattgctcca gacttatcat cccaaattgc tcttatgtgt ttggattgta
    17641 atcttcgaat tcccaagaaa aaaatcgtaa taagtttgaa agtgtcaaat cccaagtttc
    17701 ttttgagcaa tattctaata ttttatcaat ttcactttta ataatcttat gatcaaactg
    17761 ttctaatatt aatgcattag accaaaaaaa accttcttta ttacaatgat gggaaatcca
    17821 tttaggagaa caaatgcaaa gtgaaaaaat agatgagcct tgttctcctt cgattccgat
    17881 atccaaatct atccatctat ggaaattatc tggaatttcg ggggtaaatt tttcaaaatc
    17941 aatatcatat aaatttatgc tttttaaatc caatttaatc attagggctg tcctagataa
    18001 atagggaaat tcaaattaag ttagaattat ccctatgaga aaaagtcgtc taagccggta
    18061 taaacaaaat aaactcattg agctatttgt cgcaggtgta actgcaagaa cagcaacaga
    18121 gcctgacagc attgtttata cggattgtta tcgtagctat gatgtattag atgtgagcga
    18181 atttagccat ttccgtatca atcacagcac acattttgct gaacgacaaa accatatcaa
    18241 tggaattgag aatttttgga accgggcaaa acgtcattta cgcaagttta acggcattcc
    18301 caaagcgcat tttgagctgt atttaaagga gtgcgaatgg cgttttaaca acagtgagat
    18361 aaaagttctt gttccatttt aaaacaatta gtaaaatcga gtttatctta gttgtccagg
    18421 acagccccat tatttttata acaccgtgaa gccgcacagc agtttgaaca gtgatacgcc
    18481 gtttgcgggc ttacgagttt attttcccgg cctgcagttt gagcaatacg gtgatttcct
    18541 acggttaata caaatgttta cacattgata catttcattt atagttccgc ctatttgaaa
    18601 atagaaaata tgaattcgac cgcaagtaaa accctgaaag gattgtcgct ggtgtttttc
    18661 gcctctggat tctgcgccct gatttaccag gtcagctggc agaggcttct attcagtcac
    18721 ataggtatcg atttaagttc gattactgtc attatttctg tatttatggt cggcttgggt
    18781 gtgggtgcgt atttcggtgg acgcattgct gaccgttttc cttcaagtat catccccctg
    18841 ttttgcatcg ctgaagtatc catcggtctg ttcggtttgg taagcagggg tctgatttcc
    18901 ggcttggggc atcttttagt tgaggctgat ttgcccatca tcgctgctgc caatttcctc
    18961 ttattgctgc ttcctacctt tatgatgggc gcgaccttgc ccttgctgac ctgttttttt
    19021 aaccggaaaa tacataatgt tggcgagtct atcggtatct tatatttttt caacactttg
    19081 ggtgcggcac tcggatcgct tgccgccgcc gaatttttct acgtcttttt taccctctcc
    19141 caaaccattg cgctgacagc ctgctttaac cttctgattg ctgcttcagt atggctgcgt
    19201 tacagaaagg atggatatag tgaacactaa accgaatact agtttgattt atatgctttc
    19261 tttccttagc ggcttattga gcttgggtat agaagtcttg tgggtaagga tgttttcgtt
    19321 cgcagcacag tccgtgcctc aggcattttc atttactctt gcctgttttc tgaccggtat
    19381 cgccgtcggc gcgtattttg gcaaacggat ttgccgcagc cgctttgttg atattccctt
    19441 tatcgggcag tgcttcttgt gggcgggtat tgccgacttt ttgattttgg gtgctgcgtg
    19501 gttgttgacg ggtttttccg gcttcgtcca ccacgccggt atcttcatta ccctgtctgc
    19561 cgtcgtcaga gggttgattt tcccgctcgt acaccatgtg ggtacggatg gcaacaaatc
    19621 cggacgacag gtttccaatg tttatttcgc caacgttgcc ggcagtgcat tgggtccggt
    19681 ccttatcggc tttgtgatac ttgatttctt gtccacccaa cagatttacc tgctcatctg
    19741 tttgatttct gctgctgtcc ctttgttttg tacactgttc caaaaaagtc tccgactgaa
    19801 tgcagtgtcg gtagcagttt ccctaatgtt cggcatcctc atgttcctac tgccggattc
    19861 tgtctttcaa aatattgctg accgtccgga taggctgatt gaaaacaaac acggcattgt
    19921 tgcggtttac catagagatg gtgataaggt tgtttatggg gcgaatgtat acgacggcgc
    19981 atacaatacc gatgtattca atagtgtcaa cggcatcgaa cgtgcctatc tgctaccctc
    20041 cctgaagtct ggcatacgcc gcattttcgt cgttggattg agtacaggtt cgtgggcgcg
    20101 cgtcttgtct gccattccgg aaatgcagtc gatgatcgtt gcggaaatca atccggcata
    20161 ccgtagcctt atcgcagacg agccgcaaat cgccccgctt ttgcaggaca aacgtgttga
    20221 aattgtattg gatgacggta ggaaatggct gcgtcgccat cctgatgaaa aattcgacct
    20281 gattttgatg aatacgactt ggtactggcg tgcctattcc accaacctgt tgagtgcgga
    20341 atttttaaaa caggtgcaaa gccaccttac cccggatggt attgtaatgt ttaataccac
    20401 gcacagcccg catgcttttg ctaccgccgt acacagtatt ccctatgcat accgctatgg
    20461 gcatatggta gtcggctcgg caaccccggt agttttccct aataaagaac tgctcaagca
    20521 acgtctctcc cggttgattt ggccggaaag cggcaggcac gtatttgaca gcagcaccgt
    20581 ggatgctgca gcacaaaagg ttgtctctcg tatgctgatt cagatgacgg aaccttcggc
    20641 tggggcggaa gtcattaccg acgataatat gattgtagaa tacaaatacg gcagagggat
    20701 ttaaccgtct taaagggttt caggcaacgc aggttttagg taacgtcctg ctagttcaaa
    20761 aaaaccgcat cacagcagtc gggacaaaat ggtttaaaca ttttgtcccg aattcttatt
    20821 cctatatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga
    20881 tagtacggaa ccgattca
//
LOCUS       DAWHXS010000028        20696 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWHXS010000028 DAWHXS010000000
VERSION     DAWHXS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.028"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(61..237)
                     /locus_tag="WIE22_000786"
     CDS             complement(61..237)
                     /locus_tag="WIE22_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126908.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(240..1598)
                     /locus_tag="WIE22_000787"
     CDS             complement(240..1598)
                     /locus_tag="WIE22_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ4126909.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMMAKAWRENVDPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1723..3369)
                     /locus_tag="WIE22_000788"
     CDS             complement(1723..3369)
                     /locus_tag="WIE22_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ4126910.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYVAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3392..4870)
                     /locus_tag="WIE22_000789"
     CDS             complement(3392..4870)
                     /locus_tag="WIE22_000789"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ4126911.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4895..6640)
                     /locus_tag="WIE22_000790"
     CDS             complement(4895..6640)
                     /locus_tag="WIE22_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ4126912.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6701..6964)
                     /gene="ftsL"
                     /locus_tag="WIE22_000791"
     CDS             complement(6701..6964)
                     /gene="ftsL"
                     /locus_tag="WIE22_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ4126913.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6961..7947)
                     /gene="rsmH"
                     /locus_tag="WIE22_000792"
     CDS             complement(6961..7947)
                     /gene="rsmH"
                     /locus_tag="WIE22_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ4126914.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7944..8399)
                     /gene="mraZ"
                     /locus_tag="WIE22_000793"
     CDS             complement(7944..8399)
                     /gene="mraZ"
                     /locus_tag="WIE22_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ4126915.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8678..9895
                     /locus_tag="WIE22_000794"
     CDS             8678..9895
                     /locus_tag="WIE22_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4126916.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            10027..10103
                     /locus_tag="WIE22_000795"
     tRNA            10027..10103
                     /locus_tag="WIE22_000795"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10061..10063,aa:Met,seq:cat)
     gene            10111..10186
                     /locus_tag="WIE22_000796"
     tRNA            10111..10186
                     /locus_tag="WIE22_000796"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10144..10146,aa:Ala,seq:ggc)
     gene            complement(10468..11289)
                     /locus_tag="WIE22_000797"
     CDS             complement(10468..11289)
                     /locus_tag="WIE22_000797"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ4126917.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11322..11966)
                     /locus_tag="WIE22_000798"
     CDS             complement(11322..11966)
                     /locus_tag="WIE22_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ4126918.1"
                     /translation="MKLKHLLPLLLSAALSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11969..12832)
                     /gene="ftsN"
                     /locus_tag="WIE22_000799"
     CDS             complement(11969..12832)
                     /gene="ftsN"
                     /locus_tag="WIE22_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ4126919.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12938..14434)
                     /locus_tag="WIE22_000800"
     CDS             complement(12938..14434)
                     /locus_tag="WIE22_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ4126920.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14448..14768)
                     /locus_tag="WIE22_000801"
     CDS             complement(14448..14768)
                     /locus_tag="WIE22_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ4126921.1"
                     /translation="MFGKQLFEEVGSKISETIVNSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15252..16778
                     /gene="putP"
                     /locus_tag="WIE22_000802"
     CDS             15252..16778
                     /gene="putP"
                     /locus_tag="WIE22_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ4126922.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIVYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17029..20634
                     /gene="putA"
                     /locus_tag="WIE22_000803"
     CDS             17029..20634
                     /gene="putA"
                     /locus_tag="WIE22_000803"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ4126923.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4841 a         5986 c         5057 g         4812 t
ORIGIN      
        1 ggcctgcgcc cgtttgaggt tggcttgccg cccggcaagg tttcggacgg cttttgcaga
       61 ttaacgtgca gggactttct caatcacgca aattgggtaa ctccccggat ttttaccgcc
      121 gcccaagtct aaacataaat cttcataaag atacgcctgc gtccgcattc cggcataaaa
      181 cgcgccgacc agcgcggcaa tcagcaataa ggctttaaaa aaacgtcggc ttttcatttt
      241 cacttatcct ccaatgcctc gaccacttct tccatctgca taaagcgcga acctttcacc
      301 aacacggtgg cgcgttcggg caaatcgtgg cgcaacactt gaatcaacgg gtctttggcg
      361 gcgaaccaca aaccgtccgc gccaaatttt tccgccgctt cgacgctgtt gtcgccgaca
      421 aaataagccg cttcgatgcc ttggtcgcgg gcatacgcgc cgacttcggc gtgcatagcg
      481 gcggcttcgt cctcgcccag ttcgcccata tcgcccatta cgaaaatacg cggcgcaggc
      541 atacgcgcca acacgtcaat cgcggctttc atgctgtccg ggttcgcatt ataagtatcg
      601 tcaatcaggg ttgcgccctt gattccggat ttgacgttca gacggccttt gatattgctg
      661 aagcctttca aaccttccgc cacatcgttc aaactcaaac ctgcagccaa agacagcgcg
      721 gcggcagcgg cggcgttgtg gacattgtgg cggccgggaa caggcagcac cacggcggcg
      781 cgttcgtcgc cgcacaccaa atcaaattcg cacgacaacg gtttcagcac gatattttcc
      841 gcgtggacat cgccgctatc gatgccgaaa gtgcgcgtat tcaaattaag cgttgccgtt
      901 ttgaagacag ccatattggc atcttcttga ggaatcagtg ccatgccgtc tgaacataag
      961 ccttgataaa tctcgctttt cgctttggca atatcgccca ctccgtcgaa accgcagccg
     1021 acatgggcgc gcatggcgtt gttgaccaat gcggcatcgg gtttggcgat ttgtgtcaaa
     1081 accgccagtt cgccaaaatg gttcataccc atttcaatca cggcatagcg gtgtttttcg
     1141 tttaatttca acaaagtcaa cggcaatccg atgtggttgt tgaagttgcc tgccgtcgcc
     1201 aaaacggcat catcaccgaa acggcggcgc aataccgcag ccagcatttc cttcaccgtc
     1261 gtcttgccgc ccgaaccggt aatgccgaac acaaacggat ccacgttttc acgccacgcc
     1321 ttcgccatca tttgcaacgc ggtaagcgtg tcatcgactt tcaacgcgcc atccattgca
     1381 acgcaatctt cgcgcgaaac cacaaccgcc gccgcaccag cagccaatac gtcttcaaca
     1441 aaatcatgcg catcaaaccg cccgcccgcc aatgcgaaaa acacatcgcc cgcgcggatg
     1501 tcgcggctgt cggttacgat gcgcgacacg ggtttgcttt cagacggcat cggaagcttg
     1561 agggcttggc agatgaaatt taggtccagt ggtttcatat ttactttcgt taatattcgg
     1621 gcggcggaca catcggtagc ggctgatttt tttatcgcct gttttgctgt ggtaaaacac
     1681 agattatttt cccattctca ttcggcattt tttctgtacg tatcattttt tagacgtatt
     1741 tttagtcgat ttgccttttc ccgcatacca cggcgcgggg tcgtcgggca gtccgtcgat
     1801 aaaggcaagg ttattgcctt cgccctgcac atcgggaaca ttcccccaaa aatcatagcc
     1861 gtcatcgggc aactcgtcgg tttcgatacc cgtccaactg ccgaatccgc gtaaaaaatt
     1921 aaacgcgctg cgcctcactt taatcatttc gcgccgcgtg tcatcggacg aaattttaac
     1981 cagcggcacg ccgtagcttt gacgcttcca cgcaccatgt gtcaacgttc gctcgccgcc
     2041 tttaccgaca tgcatcaaac cgtggtcgga aaagtaaacc aaagaccagc tttctttatt
     2101 ttcattcaat atcttaactg tatcttctaa aaatttatcg gtttgcgcga tggtggaaac
     2161 atagcaggat attttttcag tttgatactg aaaccgccgc gcatccttat ccaaacgtgt
     2221 gcaaaaatca ctgtgcgaac ccatcaggtg catcacaatc agccgaggct tcgtgcctgc
     2281 atttcctatc aaaacccgtt tgaacgccgg caacaaaagg cggtcgctca accccgcgct
     2341 tttgccataa tcaccccttt gggtaaacca cggataatcg ctgcgtaggg cataggtgga
     2401 aatttcgttg gcaaaatgcc ccaacattcc ttgattagac agccacgccg tccgaaaacc
     2461 cgcctgcttc gccaagctga cgatgttatt gttcggttct cccggcagcc ccaaagtctg
     2521 cggcagcgaa agattcgtcg cgtgggcggt cgattggtaa ccgtttatca acagcccttt
     2581 ggtctgactc aaaaaaggcg tatcgggcaa tgggaaaccg taaacattca tataatccga
     2641 acgcgcgctc tcaccgataa ccacaacata atttttatac ttgggcgcaa catgacggat
     2701 atgccatgtt gacgcttttt ttgctgcttc caaaatgtgg gcgcgcttgg cgacatattg
     2761 tgccggcgcg gaagccaaat cgtaatacag gtcgaaaata ttcaacaaca ggccggcatc
     2821 aggttctcgc aaatctttat cgctggcgat tttatccatc accgcgcagg acaaaatcaa
     2881 agtcaataat atagtcagcc atattttgct gcggcgttta tagtttttta cgtcagcaaa
     2941 tacccccccc gatacacaat atttccaaac tgtcaagcca aaaatgaaaa ataatgcctg
     3001 cacaaaataa agcgacccgg gaagattgcc gacaaattca cgcgcctcgg caggattgct
     3061 ttccaatatc gaaccgacta tctgataaga cggcgcacca tacaaccagc cgaccggcaa
     3121 atatagggcg gttgtgccga cgtaaatcag taaaacaact gaagaaacgc gcgggaaacc
     3181 gcgtgccaat aataataaaa atacaaccga agccaaagca cccacggcag aataacggta
     3241 gccgtattca tattccaagt gataacctga tgcgatggcc gcgccaaaac aaaaggctaa
     3301 aagcgcgcaa atgcccaatg tattttttaa tgtctgatcc atatttttta ttcgggcgga
     3361 ttgtttcata cgatgccgtc cgaatatccc attatttacg agttaacaaa gcctgtccga
     3421 cgatttcaag atcggaaaaa cggtgcttca cgccttgtac atcctgatag ttttcatgcc
     3481 ctttgccggc aatcaggatg atgtcgtttg cggcggcttg ttcaaccgca taacggatgg
     3541 cggcggcacg gtcggcttcg acgcattcgg gcgcgggaac ggcaggcagg atgtcgttga
     3601 tgatgtcgtg cggattttcc aaacgcggat tgtcgctggt gacgacgact ttatccgcgc
     3661 cctgtacggc tgccgcgccc atcagcgggc gtttgccgcg atcgcggttg ccgccgcaac
     3721 cgaatacgca ccacaaagcc gcaccctgcg gtttgatttc ctgcaaggtg gcgagtgctt
     3781 tttccaatgc gtcgggcgta tgggcataat cgacaacgac caaaggtttg ccgctgttca
     3841 tgatgcagtc catgcgccct gaagcgggac ggatttttgc cagcacatcc aatacattat
     3901 caagcggata gccgttggcg cacagcaagg cgatgcaggc ggcgaggttt tgcgcgttga
     3961 accgtccgag caggcgcgtg cggcatttcc cttcgcccca cggggtttgg aatacagctg
     4021 ctatgccgtc tgaagaggcg gtgaaatcga taatgcggat gtcggcgtgt tcgctgaagc
     4081 cgtagctata aacggccaaa tcgggacagt cttttttcag acgacctacg agttccgcgc
     4141 cgtattcgtc atccacgttg atgactgcgt gtttcaagcc gtgccagtaa aacaggcgcg
     4201 acttgatggc accgtaggct tccatcgtgc cgtggtagtc gaggtggtcg cgggtgaggt
     4261 tggtaaagat tgcgctgcgg aatgacacgc cgttgacgcg cgactggtca agcccgtggc
     4321 tggagacttc catcgcggcg actgttgcgc cttgttgacg gaaacggtag agcagggttt
     4381 ggacatcgac gggggcgggt gtggtatgcg tggtttcttc caatgcaccc caaaagccgt
     4441 tgccgaccgt gccgacaatg gcggtttttt cgcccaacaa atcggcagct tgcgccagcc
     4501 attgcgtgat ggaggttttg ccgttggttc cggttacgcc ccagaatttg agaccgtctg
     4561 aaacgttgcc gtaaacttgc gccgccaata tgccggcacg gtgtttcaaa tctttgatgc
     4621 cttgattggg gactttccat tcgggattcc acgcaaattt gccgtcgtcg tcccaaaaaa
     4681 caaaagccgc gccgttggca acggcggcgg ggatataact gcgtccgtcg gcatattcgc
     4741 ccggacaggc aacgaaaata tcgccttgtt tgatttggcg gctgtctgaa tgcaacaaac
     4801 gccctgccgc gtttgcacac gacagggtcg ggatgccggt ttcagccaaa ggggttaact
     4861 tgctgaacat aaaacaatct cgttgatact cggattaaga cggtgttttg acggctgcgg
     4921 cggtcagggg cttggtcggg gaaatgccca agatgttcag gctgccgccc ataatttttt
     4981 tgaagggcgg ccctgccact acgccgccgt aataaccgtg ggcagtcggt tcgtcaatgg
     5041 ttaccgccac aatcacacgg gggtttttgg cgggggcaaa accgataaag gtagcgatgt
     5101 gtttgttgtc ggcataacgc ccgttgacga acttgcgcgc cgtgccggtt ttcgcgccga
     5161 catcgaaacc gtccaccgca cccgccgtac cggtgccgcc cggctcggtc acggaaacca
     5221 tcagattgcg tacctcgcgc gcggtcgatt ctttgaatat gcgtttgcct tgcggcgcaa
     5281 ccgcctgttt ttcaaagctg accggcagca aaacgccgtc gtgcgtcagt gcggtatagg
     5341 cgcgcgccaa ttgcagcagg ctcaattgca ggccgtaacc gaaagacatc gtcgcctgtt
     5401 caataggccg ccacctgcgc caatttctca acaaacctgc agtttcgccc ggaaagcccg
     5461 agtgcatacg cacaccgatg cccaactcat gatagaagtc atacatttct tcggcaccga
     5521 aacgcgcaga cagtttgctt gtgccgacgt tggacgattt ctgcatgatg ccgcgcacat
     5581 ccaaagaggg gtaaacatgg gtatcgcgca cgggagacgg tccgatttta taaggctgcg
     5641 tattcagccg ttcgttcaaa tcggttttgc ccgcatccaa tgctttcgca atcacaaacg
     5701 gtttgattgc cgaaccgggt tcgatcatat cggttacggc acggttgcgc cgctgttcgc
     5761 tgtctgcccg gccgggcctg ttgggatcgt aggcgggcgt attggccaag gcgaggattt
     5821 cccccgtgcg ggcatccaaa accaccaccg ttccggcttt tgcctgatgg tattcgaccg
     5881 ccttgttcaa ctcttcatag gccaaggtct gaatcctctg atcgagggaa aggatgatgt
     5941 ctttgccgtt tttcggggct ttattgcgcg gggagtccaa gctgtccaca atattgccct
     6001 gccggtcccg caaaacgact tccgcgccgt cttcgccatg caggctgtct tcaagcgaaa
     6061 gttccaaacc ttcctgacct ttgccgtcaa tatcggtaaa tccgatgacg tgtgcaaaca
     6121 ggttgcccat cgggtaatgg cgttttaatt ctttttcaaa tacaaagttt tccaaaccca
     6181 aggctttgac ctcttcggca accttgggat cgagctgccg cttaatccag ataaacgact
     6241 tgcctttctg ttcgagcttg ttcctcaaaa catcaaccgg cacatcgaca agctcggaca
     6301 ggcgttccaa ttgtgcggca gacggcattt ccttcatctc tttaggcacg gcaaacaggg
     6361 actccgtcgg cgcactcaac gccaaaaccg caccgttccg gtccgaaacc gtaccgcgtg
     6421 tagccggcaa tgtttgagtc cgcacaatcc ggttgtcgcc ctgttctttc aaaaagttat
     6481 acgttaccgt ctgcagatac agtccgcgag caatcagacc ggcaaacaag accgctattg
     6541 ccatcaggac gaagctgatc cgtccgttac tggtcatcgg ctttttgacc tgctcttctt
     6601 tgggcagcat ccgaggctta tattcgctct taatcaacat ttttacttct cgttattatt
     6661 atcctgacgc aggaatccga ttccggcaca caggctgctt ctatctttga tgcgccacca
     6721 taaaggtatt gcccgaaacc ggcggatgga ggttttgttt ttctgccgcc gccctgatcg
     6781 cttcgtggtt cgccaaacgc gcctgttgca gcctcatttg cgcataatcc tgctccaagg
     6841 cgatttcctg ttttttcgcc ttgtccaaag ctgtgaagtt gagcctgtac tggttttgct
     6901 gcatcacaac ggaaaaagcg gaaacgcaca ccgcaagcag cagaaggaaa ttcaatttgt
     6961 tcattgccat tcagacgttt ttctctgtga ttgttccggt atcgaaccgg cagtccgctc
     7021 cgccacacgc aaaaccgcac ttctcgccct cggattggcg gcaatttccg cctcacccgg
     7081 ctttaatgcc ctgcccacga ttttcagggg cggctcgggc aaatccgctt ccctgaccgc
     7141 cgcccagcgc ggcaggggcg cgtgttgcga atattttttg acaaactgct tcacaatgcg
     7201 gtcttccaac gaatggaaag caatgaccgc caaacgtccg ccctctttca gacgacacat
     7261 gacctgcggc aatactgccc ctacttcttc aagctcgcgg ttaataaaga tgcggaccgc
     7321 ctggaaggtg cgcgtcgcag gatcctgccc ccgctcgcga gtacggacgt tttgtgccac
     7381 gatctgcgcc agcttgcggg ttgtatcgat tggactttcc gcccgttgcg caacaatggc
     7441 gcgcgcaatc tggcggctaa accgctcttc accataattc ttgattacct cgtgcaaatc
     7501 ctgttccgac gcaaccgcta tccactctgc ggcagacata ccgcgcgtcg tatccatacg
     7561 catatccaaa ggggcatcga aacggaagct gaaaccgcgg ctgccgtcat cgatttgcgg
     7621 ggacgaaatc cccaaatcaa acagcgcacc gtccaccttg ccgataccca aaccgtccaa
     7681 tgccgtctga aacgaagcaa aaccgccatg cacgacaccg acccgtttgt ccgaacgcgc
     7741 cagctcttct gccacagcaa tcgcctgagg gtctttgtcg aaaacaatca accgccccgc
     7801 atcgcccaaa cgcgacaaaa tcagccggga atgccctccc ctgccgaacg taccgtccac
     7861 atagacaccg tcttcgcgca cggcaagcgc atccaccgcc tcattcagca agaccgtgat
     7921 atgccgataa ctttctgctc cactcacaat tgcaaatccg tctgacccaa ctggaaggca
     7981 agttcgtcag gatcgtcatc caaagcctga accatctcag cctcccactg ctcgcgaccc
     8041 caaagctcca aacggttggc acgaccgacc aaaacgactt cacggtcgaa atccaccctt
     8101 ttcctcagtc cggcaggaag cagcacccgg ccggcgctgt cccattccaa aatttccgcg
     8161 ttatgcagca aaagattttg aaaccgccgc aaaacagggt tatccgccac ttttaagttt
     8221 aaaagttgcg ccgcaacctt ttcccactcc gcaacagggt acatcaacag cttgtgtttc
     8281 gactcgagcg ttaccactac ggcaggcgta tagaggcgcg acagaatgtc acggaatttg
     8341 gcaggaacag ccaaccgccc cttactgtcg atgcttaatt cgtgtgcgcc gccgaacatg
     8401 acatgtccca agccgaaatc aaaatcacaa gggtaaaaga gacactttgc cccacaattc
     8461 cccaccaatc gacactataa gaaattttaa acactcggtc aaatcagggc atgaaaactc
     8521 attaacatat ctgaaatttt tattcctttt aaaaacaata agataaaaaa tgacgacaac
     8581 ggcacggcgg gtgcggtaca gaataatcga accaataaac aactatatat gattaattta
     8641 ataatataaa cacaatatat agtattaaga taaagccatg acagcacccg taccaacgtg
     8701 taatatgtcg ggaaatccaa taaatttaca caagctaaca cttatcatgc ccctccccct
     8761 cccctctccc gaagcacggc aattctcgct caaactgcaa accctcattg ccgaaaaaat
     8821 cggcaaacac ggcaactgga ttccattttc acgttttatg gaattggttt tatacgctcc
     8881 gcaatacggc tactacaccg gcggcagcca taaaatcggc aataccgggg attttattac
     8941 tgcaccgacc ctcacctctc tgtttgcaca gacactggca cgccaacttc aagaacttct
     9001 atcccaaacg gcgggcaata tctatgaatt cggcgcggga accggacagc ttgccgccga
     9061 tttgttgggc agcatttcgg acggcatcag ccgttactat attattgaaa tatcgccgga
     9121 gctggcagca cgtcagaaaa acctgattca agcccgcgca ccggaagcat ctcaaaaagt
     9181 tgtccacttg accgcacttc ccgaagcgtt tgacggcatc atcatcggca acgaagtact
     9241 cgatgccatg cctgtcgaaa tcgtccgtaa aaatgaaggc ggctcattcg agcatgtcgg
     9301 cgtttgccta gataatgacc gttttaccta ttcggcacga ccgctgcacg acttacagct
     9361 atctgccttg gcttccctct attttcctca aacagattat ccctacacca gcgaactaca
     9421 tccgcaacaa tatgccttta tccgcaccct tgcctcaaga cttgaacgcg ggggtatgat
     9481 attcatcgac tacggttttg atgcagcgca gtattaccac cctcaacgca atcaaggtac
     9541 tctgatcgga cactaccgac atcacgttat ccacaatcct tttgacttca tcgggttggc
     9601 cgacctgacc gcgcatgtca actttaccga cattgcccaa gccggtacgg atgccggatt
     9661 agatttgata ggttaccttc cccaatccca tttcttattg aacctgggca ttaccgagct
     9721 attggcacag acggggaaaa cggattcggc agcctatatc cgtgaagctg ctgccgttca
     9781 gaaactgatt gaccagcatg aaatgggcga actgtttaaa gtcatcgcat tcggaaaaaa
     9841 tatcggcgtc gactgggcag gattccgctt cggcgacatc tgccacaaac tctaaccctc
     9901 atgccgcctg aatccgcttc agacgacata aactttttaa catttaaaaa cagtcaacta
     9961 attcaaaatt aaaaaatacg gcttgtcaaa aaaacagaaa aacatataat agcgtcttca
    10021 cgaaacggcg aattagctca gtcggttaga gcagaggaat cataatcctt gtgtccgggg
    10081 ttcgagtccc tgattcgcca ccaaattttc gggggtatag ctcagttggt agagcgcttg
    10141 catggcatgc aagaggtcag cggttcgatt ccgcttacct ccaccagata aaaaagcaca
    10201 gaccgtaaaa aggtatgtgc ttttttattg cctgattgcc agcaaataaa gaataaacca
    10261 ctgccttcaa aacaggcaat cgactttaaa cctatcgccc cgcctgtcct gattttatag
    10321 tgaattaaat ttaaaccggt acagcgttgg ctcgccttgc cgtactatct gcggcttcgt
    10381 cgccttgtcc tgatttttgt taattcacta tatcagcccg ccagacaaac ccgacccgaa
    10441 taatgtcttc aggtcgggtt tatggtttca ttcccaactt atccagcctg acagccacaa
    10501 tataatgatg gcaataccaa aaacaatgcg gtaataggca aaaggaatat aatttttctt
    10561 ggaaacaaac ctcagcaacg cttttaccgc taccaagcct gaaacaaagg cagcaataaa
    10621 gcctatcaga atcaaaccga catcatgcag ggtgaaaaat cggtaatgtt tcaggacatc
    10681 ataagccgtt gcggcaacca tcatcggcac agccaagaaa aacgagaatt ctgtcgcagt
    10741 tttccgttcg atgccccaaa gcatcccgcc cataatcgta ctgcccgaac gggacgtacc
    10801 cggaaccagt gcaaacactt gggcaacgcc gatcatcaag gcatcaatcg gacgcaatgc
    10861 atcaacatcg gcaattttag gctctgctcg gctttggcgt ttctccaccc acaaaataaa
    10921 aaaaccgccc aaaaccagca tgactgcaac actcaagggg ttaaacagat actctttgat
    10981 ttgtttgccg aacaacagcc ccatcacggc ggcaggtata aaagcaatgg caagattaag
    11041 gacgaagcgg ttggctttcc ggtcttttcc caagccgtgc aacacattgc tgaaacgttg
    11101 ccggtattca aacactaccg ccaaaaccgc accgagctgg atggcaattt caaaaacctt
    11161 gtgattgctg tgaaaaccaa tcagattgcc gaacacaatc aaatgtccgg tgctggaaat
    11221 cggtaaaaat tcggtaaaac cttctaccaa gcccatcatc agggctttca ggacaatcag
    11281 aaaatccatt gcttgcgctt ctttcggata cgggagttcg gctatttctg tacagcaggg
    11341 gtctgacgct tgcgttcttc cctgactttg gcaaccaatt ctttaatcgt atgaacgccg
    11401 ccgtcaaagc cgttattgaa gataacgcgg tattttccgc cgacaataac ggtcggcgtg
    11461 ctgtcgatgc ggtattgttc cgtcagtttc tgcattttta atgcggcggc ggcagcttcg
    11521 ggggaatcat aggcgcgcat cagttttttg ccgtcaaagc ctttttgaga caaagcccat
    11581 tttccggcaa cagccctgtt ttccaagcgg attttttgtt cgtaaactgc tttaaacacg
    11641 gcagggtttg cctgatattt caaacccgac aaattgacgg cagccgccat cctggccaga
    11701 ccgagcattt caggctgcca gaccacgtgc tccgtcctca aataggcatc agacggcaat
    11761 gccttgccca gtttcaataa caaaggatcg aaatgatggc aatgtacgca gaaatagccg
    11821 aaaaattcca aaacctcaat tttacccgac tgttcttgag gaatgggttt atccaacaca
    11881 agatagtctt ccccttccgt cagggcatat gcctgcgcgg acaacgctgc cgacagcagc
    11941 agcggcaaca gatgtttgag cttcataatt atttactttc gatagaacgg atcaggctgg
    12001 cgacttcatg ttttttcaac tcgtcctgca tttttttcac cgcatcggca gacatattgc
    12061 cgctttgcac ccggtaaagc gttttatgtc ccgcctgata accgaccacc ttggaagata
    12121 tgcccaagat tgccagtttg gcacgctgtc cttccgcgct ccggcggtcg gcatacgcgc
    12181 ccatttgcag ataatgcgtt gcttccgcct tgtcgggcgt tttcattttc tgcacttctt
    12241 tggcagcggc actgcgcgct ttttcgatgc tgccgctgtt gaggatttgt tccggggtcg
    12301 gtttgggtgc aactttttcc ttctccgcct ttttctcttc ttttgaagct tttttctctg
    12361 tttctttaga tggttttacc gcttgttttt taaccgtttc ggcatctttc ttctgcgctt
    12421 tttccccgac ggtttgttca cgctcttccg tcagtgcttt cttgcgcact gcctgtccgt
    12481 ccgacttttc ccgctccggc tcgtccgcct tttcctcaac ctcgtcggct ttgtcggcaa
    12541 cgggctgctt gtcggcagct ttttccgcat ccgactgctc tgcctctttc gcagcatccg
    12601 gttcggacaa ggcgttttga tcggccggtt caggttggat gtcttcctta ggctggtttt
    12661 tcggtttcag gatttccgtt tctgcaggct gcttcgacgg aaccgggatt ttgaacgcat
    12721 tttgaccgct ctggttcaga taaaacaaaa taccggcaat aatgaccgtc gccagtatca
    12781 aaccgaagaa aaaaccggac agaccttttc cggattggga aaatttgttc ataaacatac
    12841 cttaatgtgt ttcagacggc attaacgccg tttgcttacg ggcggatatt ctaacaatat
    12901 cgccatattt gggcaaaacc tgcttccatt cccattccta taaagcacga cggaaaccta
    12961 tggctttcat gacgtggctt ctgccgactt cttcgtcgcc cgccaaatcc gccaatgtac
    13021 gcgccacgcg cataatgcgg tggaagctgc gggcggaaag ggagagtttt tccagcaggc
    13081 cgcccaatgc ttcctgcgct tctttttgaa tgcgggcttg ggaatcgagt tcgctgacac
    13141 tcaaagcggc gttgactttg ccctgccgcg cgtattgttt gtcgcgggcg gcgataacgc
    13201 gttccaaaac ggacgcgctg ctttcacccg cttcctgctg catcagttcg gcggcggaca
    13261 ggctggggac ttcgatggtc aaatcgatgc ggtcgagcag cgggccggaa attttgctgc
    13321 ggtaacgcgc gacgctttcg ggcgtgcagc ggcagggttt gacgggatgc ccgagataac
    13381 cgcacgggca ggggttcatg gcggcaacaa gttggaattt ggcaggatag acggcttggc
    13441 gcgccgcgcg ggaaatgtgg atttcgccgt tttccaacgg ttcgcgcaaa acttccaaaa
    13501 ctttgcggtc gaactcgggc agctcgtcca aaaacaatac gccgtggtgc gccaatgaaa
    13561 tctcgcccgg acgcgggtcg gaaccgccgc cgaccatagc cgccgcgctg gcactgtgat
    13621 gcggactgcg gaaaggacgg tggctgtcga gttgttgctg gtggttgggc aggagcgaac
    13681 gcaatgccca aacttcaacc aattcgtctt cggtcagcgg cggcaggatg cctggcagcc
    13741 gttgggagag catagacttg cccgttcccg gcggacccat catcaagagg ctgtgtccgc
    13801 ctgcggcagc gatttccaaa gcaaggcggg cagtgtgctg acctttcaca tcacacaaat
    13861 caggttgtcc gccatgttca gacggcattt gcggtacgga acattcggtt tgcgccaaag
    13921 gttcgatacc gttcaaatgg gcggcgactt cgcccaaaga gcgcgcgccg taaacggtaa
    13981 tgccgcgcat cacggcggct tgtcctgcgt tttcttcagg caaaacaaat gcacgttttg
    14041 cctgcatacc ctgccacgcc atcgccaacg cgccgcgcac ggggcgcaac agccccgaca
    14101 gtgccaattc ccccgcaaac tcgtattccg ccagtttttc gggcgcaacc tgccccgatg
    14161 cggcaaggat gccgattgca atcggcaaat cgaaacgccc cgactctttg ggcaggtcgg
    14221 cgggggcgag gttgacggtg atttttttgg cggggaattc aaaaccgctt tgaataatgg
    14281 cggcacggac gcggtcgcga ctttctttga cttccgtatc gggcagtccg acgatgttga
    14341 aatgtggcag gccgttggca aggtgggctt ccacttcgac caacggcgca ttcataccgc
    14401 tcaaggcgcg gctgtaaacc aaggcaagcg acatatttca gacggcctta ttcgccggct
    14461 tcggtttgct gcctgatttc ggcgacggct tcttcggcag cggcttcagc cgcttccaat
    14521 gctgcccgtt cgggattttg cgcggcttcg agttttgcca aacgcgcttc caaagccgcc
    14581 agtttggtac gggttttgat taaaacctgc tgctggatgt cgaattcttc gcgcgtaacc
    14641 aaatccatac ggttgaacgc gccgcccagc atcgccttaa tatttttttc cacatctttg
    14701 gcagggctgt tgacgatggt ttcgctgatt ttcgaaccga cttcttcaaa aagctgcttg
    14761 ccgaacataa tctgtatcct tcctgaacat atcaaattca atcggctatt gtataaggaa
    14821 aaatgccgtc tgaaaacggg cggcggataa tcggcaaaac ataccgcgcc tcctttgcgg
    14881 ttgctaaatt acacctcaaa acacctgccg cctgaaaacg gatttcatat tccgcgcaac
    14941 gccagattac aagacactac aaaacaatat actgttttaa atgatttttc cgacgcgcat
    15001 cgtttcaaac tcggcttttt aagccattaa gtgctttgca aacaacagaa attgggttat
    15061 gctgaaacgg attatttaca atttcatata gttttattac atatcttatt gtgattgaag
    15121 ataatttatc cgaatccccc tttcgggtat ccggattttc cgttgtactt ttatttagaa
    15181 aaacatttca ggcgcaagtt gcttgcaatt tcaaagcctg taattctgaa gttttagacg
    15241 aggaatagca catgaatccc atgtacatta cttttgcaat ctatctggtt gcagtccttc
    15301 tcatcgggct tgccgcctat ttttctacgc gcaatttcga cgattatatt ttgggcgggc
    15361 gcagcctagg cccgtttgtt accgcgatgt cggcaggcgc gtccgatatg tccggctggc
    15421 tgctgatggg tctgccgggc gcgatttacc tgagcggttt gaatgaggct tggattgcca
    15481 tcggcctctt ggtcggcgcg tatttcaact ggctcttggt ggcgggccgc ctgcgcgtgc
    15541 ataccgaata cgccaacaat gcgctgacgc tgccggatta tttcttccac cgcttcggcg
    15601 cgggcggaca cttgatgaaa gtggtttccg cactaattat cttgtttttc ttcacaattt
    15661 actgtgcttc cggcattgtg gccggcgcaa ccctgttcca aagtctgttt gaaggtatga
    15721 cttacaacca ggcaatgtgg ctgggtgcgg gcgcgaccat cgtctatacc ttcttgggcg
    15781 gtttcttggc agtcagctgg acggatacgc tgcaagcatc tttgatgatt ttcgccctga
    15841 ttttaacgcc ggtgatggtc tatctgggct tgggcggcgc ggaacagatg tctgccgcga
    15901 ttcaaagcgt cgccgcaggc acgggcaaag aatacggcag cctgtttgcc ggtacgaccg
    15961 tcatcggcat catttccacc gccgcatggg gcttgggcta tttcggacag ccgcacattt
    16021 tggcgcgctt tatggcggcc gaaagcgcga aatcgctggt atccgcacgc cgcatcggca
    16081 tgacttggat ggcgttgtgc cttgcgggcg cggtagcggt cggttatttc ggcattgcgt
    16141 atttcggtgc aaatcccgac aaagtttctt ctatgagcgg caaccacgaa cgcatcttca
    16201 tcgcgctttc caccctgctg ttcaaccctt ggatcgccgg cattattttg agcgcgattt
    16261 tggcggcggt gatgtccacc ctgtcctgcc agcttttggt ttgctccagc gcgattaccg
    16321 aagacttcta caaaggcttt ttgcgtaaaa acgcgcaaca gtcggaactg gtatgggtcg
    16381 gccgcctgat ggtcttggcg attgccgtga tttccatcct gattgcttcc gatccgaaca
    16441 gcaaagtatt gggactggtg tcttacgcgt gggcaggttt cggtgcggca ttcggcccga
    16501 ttgtgattct gtccgtattg tggaaacgca tcactgccta cggcgcgttg tcgggtatgg
    16561 ttgcaggtgc cttaaccgtg gtcgtatggg cggaatgggt gaaaaaaccg gctcaagcgg
    16621 caggcgaaag cggcttgttg acgatgtacg aaatcgtccc gggctttatt gtttgcttga
    16681 tcgttgccgt gttggtttcc ctgttcaaca aagaaccttc gcgcgaaacc caagaacgct
    16741 ttgaaaaagc cgacgcagat taccgcgccg cccgataaac ccctttcaga cggcacggca
    16801 tcccggttcg tgccgtctga acccgtttcc gggtcaaaca caaataaacc ttgcctgcat
    16861 acaggcctta tttgtgtttt acccgtttca ttttcaaaga aacggaagca cctaccctgc
    16921 ccgcatttga agccttgtat ttcatctgcg gatttattac gatattaccg tattcagacc
    16981 gcaccgatgc cgtctgaacc ccccgaaaaa ctttggagaa tccaaaaaat gtttcatttt
    17041 gcatttccgg cacaaactgc cctgcgccaa gcgataaccg atgcctaccg ccgtaatgaa
    17101 atcgaagccg tacaggatat gttgcaacgt gcacagatga gcgacgaaga gcgcaacgcc
    17161 gcctccgagc ttgcccgccg tttggttacc caagtccgcg ccggccgcac caaagccggc
    17221 ggcgtggatg cgctgatgca cgagttttca ctctccagcg aagaaggcat cgcgctgatg
    17281 tgtctggcag aagccctgct gcgtatcccc gacaacgcca cgcgcgaccg cctgattgcc
    17341 gacaagattt cagacggcaa ctggaaaagc catttgaaca acagcccttc cctcttcgtc
    17401 aacgccgccg cctggggcct gctgattacc ggcaaactga ccgccacaaa cgacagacaa
    17461 atgagttccg cactcagccg cctgatcagc aaaggcggcg caccgctcat ccgccaaggc
    17521 gtaaattacg ccatgcgcct gttgggcaaa cagttcgtaa ccggacagac cattgaagaa
    17581 gccctgcaaa acggcaaaga acgcgaaaaa atgggctacc gcttctcctt cgatatgttg
    17641 ggcgaagccg cctacaccca agccgatgcc gaccgctact accgcgacta tgtcgaagcc
    17701 atccacgcca tcggcaaaga tgcggcagga caaggcgttt acgaaggcaa cggcatttcc
    17761 gtcaaactct ccgccataca tccgcgctac tcgcgcgccc aacacggccg tgtgatgggc
    17821 gaactgttgc cgcgcctgaa agagctgttc cttttgggta aaaaatacga tatcggtatc
    17881 aacatcgatg ccgaagaagc caaccgtctg gagctgtctt tggatttgat ggaggcactc
    17941 gtttccgatc ccgatttggc aggctacaaa ggcatcggtt tcgttgttca agcctaccaa
    18001 aaacgttgtc cgttcgttat cgactacctg atcgaccttg cccgccgcaa caaccaaaaa
    18061 ctaatgatcc gcctcgtcaa aggcgcgtat tgggacagcg aaatcaaatg ggcgcaagtg
    18121 gacggcttga acggctatcc gacctacacc cgcaaagtcc acaccgacat ctcctacctc
    18181 gcctgcgcgc gcaaactgct ttccgcgcaa gacgcggtat ttccgcaatt cgccacccac
    18241 aacgcctaca ccttgggcgc aatctaccaa atgggcaaag gcaaagactt tgaacaccaa
    18301 tgtctgcacg gcatgggcga aaccctgtac gaccaagtcg tcggcccgca aaacttaggc
    18361 cgccgcgtgc gcgtgtacgc cccggtcggc acacacgaaa ccctgctcgc ctacttggtg
    18421 cgccgcctgt tggaaaacgg cgcgaactcg tctttcgtca accaaatcgt cgatgaaaac
    18481 atcagcatcg acacgctcat ccgcagcccg ttcgacacca tcgccgaaca aggcatccac
    18541 ctgcacaacg ccctgccgct gccgcgcgat ttgtacggca aatgccgtct gaactcgcaa
    18601 ggcgtggact tgagcaacga aaacgtattg cagcagcttc aagaacagat gaacaaagcc
    18661 gccgcgcaag acttccacgc cgcatccatc gtcaacggca aagcccgcga tgtcggcgaa
    18721 gcgcaaccga ttaaaaaccc tgccgaccac gacgacatcg tcggcacagt cagctttgcc
    18781 gatgccgcgc ttgcccaaga agcggttggc gcagccgttg ccgcgttccc cgaatggagt
    18841 gcgacacctg ccgccgaacg cgccgcctgc ctgcgccgtt tcgccgacct gctcgaacag
    18901 cacacccccg cgctgatgat gcttgccgtg cgcgaagcag gcaaaacgct gaacaacgcc
    18961 attgccgaag tgcgcgaagc cgtcgatttc tgccgctact acgcaaacga agccgaacat
    19021 accctgcctc aagacgcaaa agccgtcggc gcgattgtcg ccatcagccc gtggaacttc
    19081 ccgctcgcca tctttaccgg cgaagtcgtt tccgcattgg cggcaggcaa caccgtcatc
    19141 gccaaacccg ccgaacaaac cagcctgatt gccggttatg ccgtttccct catgcacgaa
    19201 gccggcatcc cgacttccgc cctgcaactc gtcctcggcg caggcgacgt gggtgcggca
    19261 ttgaccaacg atgcccgcat cggcggcgtg attttcaccg gctcgaccga agtggcgcgc
    19321 ctgatcaaca aagcccttgc caaacgcggc gacaatcccg tcctgattgc cgaaaccggc
    19381 ggacaaaacg ccatgattgt cgattccacc gcacttgccg aacaagtctg cgccgacgta
    19441 ttgaactccg ccttcgacag cgcgggacaa cgctgctccg ccctgcgtat cttgtgcgtc
    19501 caagaagacg ttgccgaccg tatgctcgac atgatcaaag gcgcgatgga cgaactcgtc
    19561 gtcggcaaac cgattcagct cactaccgat gtcggccccg tcatcgatgc cgaagcacag
    19621 caaaacctgt tgaaccacat caacaaaatg aaaggcgttg ccaagtctta ccacgaagtc
    19681 aaaaccgctg ccgatgtcga ttccgaaaaa tccacgttcg tccgccccat cctgtttgaa
    19741 ttgaacaacc tcaacgaact gcaacgcgaa gtcttcggtc ccgtcctgca cgtcgtccgc
    19801 taccgcgccg acaaactcga cagcgtcatc gaccaaatca acagcaaagg ctacgccctg
    19861 acccacggcg tacacagccg catcgaaggc acagtacgcc acatccgcag ccgcatcgaa
    19921 gccggcaacg tttacgtcaa ccgcaacatc gtcggcgcag tcgtcggcgt acagcccttc
    19981 ggcggacacg gtctgtccgg cacaggcccc aaagcaggcg gttcgttcta cctgcaaaaa
    20041 ctgacccgca tccccgaatg ggttgccccg accctgagcc aaatcggaca ggcagacgaa
    20101 gccgcgctca aacgcctcga agcactggtt cacaaactgc cgttcaacgc cgaagagaaa
    20161 aaagccgcag cggccgcttt gggacacgcc cgcatccgca ccctgcgccg tgccgaaacc
    20221 gtccttaccg gcccgaccgg cgagcgcaac agcatctcat ggcacgcgcc aaaacgcgtc
    20281 tgggtacacg gcggcagcct cctgcaagcc ttctgcgccc taaccgaact tgccgcctcc
    20341 ggcgtacagg cagtggtcga acccgacagc ccgctggctt cctacaccgc cgacttggaa
    20401 ggtctgctgc tggtcaacgg caaacccgaa accgccgaca tcagccacgt tgccgccctg
    20461 tcgcctttgg acagcgcgcg caaacaggaa cttgccgccc acgacggcgc actcatccgc
    20521 atcctccctt cggaaaacgg actcgacatc ctgcaagtgt ttgaagaaat ctcttgcagc
    20581 gtcaacacca cagccgccgg cggcaacgcc agcctgatgg cggttgccga ctgattttac
    20641 cgaaatcccc gagcgcggcc cgtgaaccaa tgccgtctga aaacctttca gacggc
//
LOCUS       DAWHXS010000029        20427 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWHXS010000029 DAWHXS010000000
VERSION     DAWHXS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.029"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(483..854)
                     /locus_tag="WIE22_000804"
     CDS             complement(483..854)
                     /locus_tag="WIE22_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126924.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIKIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(988..1431)
                     /locus_tag="WIE22_000805"
     CDS             complement(988..1431)
                     /locus_tag="WIE22_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126925.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1474..2343)
                     /locus_tag="WIE22_000806"
     CDS             complement(1474..2343)
                     /locus_tag="WIE22_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ4126926.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNSKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLINPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2507..3430
                     /locus_tag="WIE22_000807"
     CDS             2507..3430
                     /locus_tag="WIE22_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ4126927.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3508..4149)
                     /locus_tag="WIE22_000808"
     CDS             complement(3508..4149)
                     /locus_tag="WIE22_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ4126928.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4204..4995)
                     /locus_tag="WIE22_000809"
     CDS             complement(4204..4995)
                     /locus_tag="WIE22_000809"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ4126929.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARSLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            5161..5236
                     /locus_tag="WIE22_000810"
     tRNA            5161..5236
                     /locus_tag="WIE22_000810"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5194..5196,aa:Thr,seq:tgt)
     gene            5271..5346
                     /locus_tag="WIE22_000811"
     tRNA            5271..5346
                     /locus_tag="WIE22_000811"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5304..5306,aa:Thr,seq:tgt)
     gene            5488..6279
                     /gene="panB"
                     /locus_tag="WIE22_000812"
     CDS             5488..6279
                     /gene="panB"
                     /locus_tag="WIE22_000812"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ4126930.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVACPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMHGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6269..6445
                     /locus_tag="WIE22_000813"
     CDS             6269..6445
                     /locus_tag="WIE22_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126931.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            6402..7238
                     /locus_tag="WIE22_000814"
     CDS             6402..7238
                     /locus_tag="WIE22_000814"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ4126932.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYCELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7403..9247
                     /locus_tag="WIE22_000815"
     CDS             7403..9247
                     /locus_tag="WIE22_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126933.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVGKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGRKKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9387..9968
                     /locus_tag="WIE22_000816"
     CDS             9387..9968
                     /locus_tag="WIE22_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ4126934.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9978..10823
                     /gene="ispE"
                     /locus_tag="WIE22_000817"
     CDS             9978..10823
                     /gene="ispE"
                     /locus_tag="WIE22_000817"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ4126935.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            10831..10906
                     /locus_tag="WIE22_000818"
     tRNA            10831..10906
                     /locus_tag="WIE22_000818"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10864..10866,aa:Gln,seq:ttg)
     gene            10919..10994
                     /locus_tag="WIE22_000819"
     tRNA            10919..10994
                     /locus_tag="WIE22_000819"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10952..10954,aa:Gln,seq:ttg)
     gene            11003..11078
                     /locus_tag="WIE22_000820"
     tRNA            11003..11078
                     /locus_tag="WIE22_000820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11036..11038,aa:Gln,seq:ttg)
     gene            11245..12228
                     /locus_tag="WIE22_000821"
     CDS             11245..12228
                     /locus_tag="WIE22_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ4126936.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            12295..12867
                     /locus_tag="WIE22_000822"
     CDS             12295..12867
                     /locus_tag="WIE22_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ4126937.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(12992..14161)
                     /locus_tag="WIE22_000823"
     CDS             complement(12992..14161)
                     /locus_tag="WIE22_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ4126938.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            14309..15835
                     /gene="ilvA"
                     /locus_tag="WIE22_000824"
     CDS             14309..15835
                     /gene="ilvA"
                     /locus_tag="WIE22_000824"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ4126939.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(15892..16965)
                     /locus_tag="WIE22_000825"
     CDS             complement(15892..16965)
                     /locus_tag="WIE22_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ4126940.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(16962..17822)
                     /gene="cysW"
                     /locus_tag="WIE22_000826"
     CDS             complement(16962..17822)
                     /gene="cysW"
                     /locus_tag="WIE22_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ4126941.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(18074..18910)
                     /gene="cysT"
                     /locus_tag="WIE22_000827"
     CDS             complement(18074..18910)
                     /gene="cysT"
                     /locus_tag="WIE22_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ4126942.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            19200..19532
                     /locus_tag="WIE22_000828"
     CDS             19200..19532
                     /locus_tag="WIE22_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ4126943.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGTESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GEACACGH"
     gene            19864..20373
                     /locus_tag="WIE22_000829"
     CDS             19864..20373
                     /locus_tag="WIE22_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ4126944.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         4927 a         4683 c         5632 g         5185 t
ORIGIN      
        1 tcctagttat ctaggacagc ccctaaactt tttggaagca tctctaattt attaatatcg
       61 ttcatatccc ctacacacag aaaaaatctt ggggaatttc ctttaatctt tttaataaat
      121 cttcacaaag cctaaaatga tttaatttta gtttcttttt atttttttga taaattctag
      181 ataacctcgc ttattaggaa aaatttcagg atatattcct ttttcttcta aagaagtata
      241 gtggattaaa tttaaatcag gacaaggcga ggaagccgca gacagtacaa atagtacggc
      301 aaggcgaggc aacgccgtac taatccacta tatatatcca ttcatataga gaaaaattct
      361 tatacccaat ataaaacaag gtctttcatt atattttgaa aaacctagaa gctttgtatc
      421 tattggttta tcaaaattaa taattattgc agaagacatg taggctaata atcttgattt
      481 atctatattt tattaattat atatttaacc ccgaagtcaa tatagaaatt tagtgatttt
      541 aaagttctct tatcaatttt aatctcaggg agtacataaa aatcctcaga ataggcaaac
      601 tctatatatg catcatcatt cgataataat tcaataatat ttttcatttc atccgaaaga
      661 aatgtaacca tctcatattg acatttattt ttagatacca tttttgaata ttgattaaat
      721 aattttggat tactattttt agtaattaat ccttccggag taataaaacc actttgtata
      781 aagtcatcac ttatattaac agccttccga aaaaaaatat aatcacactc tttgttatca
      841 atataaaatc tcatactctt taataatgaa atataagaat ttccagaatt acaagcaatt
      901 tgaaaaaaca tcattactcc taaaactcat aaatagtaag gaatatatat atatgcatat
      961 tccttactat tttttcacaa attatgctta tctatgctta tttccataaa aagcatgccc
     1021 tcgaattttt tgacttgagt tctggaaatg agaataattt gaatggattt ccttctgtcc
     1081 atgtctaata acattttgtc ttccccaagc ttggtttgct accgctcttg cttgttgaga
     1141 tgttcgtgca tagacattag ctcctcttga taaagcttgt gctgcaacac gctgcgtaac
     1201 ccaacgctgc gcaaggaatc tgcccgcatg caaaattcct attgccacca acggtgccgc
     1261 agccccttca gtttctttca tctccttttg agaaagctcc gccaactgca tcggcgcatc
     1321 tgcctgcgtg tggaacactt ggttttcaaa tgtctgattg tccaagccgt ttgccattgc
     1381 gggggcaatc atagacagca tcattacggc tgcggtgatt tgttttttca taataactcc
     1441 tttggattac aaggttggaa aatcaaaacc tgcttaaaat gtatgctgta cgccaaattt
     1501 cagttcggaa ctgctttgcc ctgaaacgtt gaaacgtgcg gatgcgttta aagccgtggt
     1561 tttggtgaaa ccgaaacctg tgccgaaatg agcgtaggtg gatgtgtttc ttgcggtttc
     1621 ttttttgccg tctatgcggt cgggctgtgt gcccagccat tggatgcctc cggtcaggct
     1681 gattctgtcg ttggcggcaa atgagatgtt ggggttgatc agcaggtagt tgcccgattt
     1741 gtagcggatg ccgtctgaaa gggttttgct gccgttgata cggtaggcgg cggtgaggga
     1801 aaggacaatc ggatctatgg ctttgtaggt ggtggcgccg atgagccagg attttcccga
     1861 cgaggctttg ttgcgcgatt tttcgtaaac cgtgctttca agaaagctga ttagggcggg
     1921 gtttttgtcg tctttaagga aagtgtggct gatgccgagg gatacgtcgg acatccgttt
     1981 gttgcgggtt ttgctgttgc cgtcgagttt gcgttcttcg tgccacagat agctgccgct
     2041 gccgtaaatg tcggtattcc cggtcagtcc gtagcgcaaa ccgagcgtgc caacgagcat
     2101 atcggtattg ctgccgtttt cttggatttc ggtcggaatg gggataaacg aggttgcgcc
     2161 ggtttggatg taaaccggtg cggcaagttc ggcgcggttg ttttcgctat tcaggtaggt
     2221 aagggaagtt tccagtttcc atttgccctt gtcagtcatt atgtcttcaa tcgtcaaggg
     2281 caggtcggca taagcggata aaggcaggat ggcgggcaag gcgggcaaaa agatgcgctt
     2341 catatttctc ctattgatat taattcttat ttactgataa taatgaatat ccgataatct
     2401 tgtctaaata aatttttaaa tcataaaaaa caatatgttt gtttttgtca attatgtttc
     2461 gatatgccgt ctgaaaagtt tgtgaaaaac ggttacaatc cgccgcatga aaaaacgcaa
     2521 taatcctctt ccgctgttga acggtgtcaa acccagttat ttggtgctgc cgcatgaaaa
     2581 gcagttttac gggctgccgc tgctgcattt tctgtgcatc cgctttcctt ttgtgggcgc
     2641 ggacgattgg cgcaggcggt tgaacagcgg ttttgtggtc ggttcggatg gtgcggcgtt
     2701 ggacgaacat tctttgttcg agccgggtaa ggtgatgttt tattaccgtg aaaccagccg
     2761 tgagagcgag ccgcgtattc cgtttgaaga aaagattttg catattgatg agcatttgat
     2821 tgtggtggac aaaccgcatt ttctgcccgt catccccagc ggcaggtttt tgcgggaaac
     2881 cctgctcacg cgcctgcgtt tgcggcctga attgcagcat ttgaatgttg aggacattac
     2941 gccgctgcac cgcttggaca aggatacggc aggcgtgatg ctgctgtcgc ataatcctgc
     3001 cacgcgcgga gcctatcaga cgatgtttca aaacaaaacg gtatggaaaa cgtatgaggc
     3061 gcttgcgccg acaaggacgg atttgccgta tccgctcgat gtggtttcgc gtttggtgag
     3121 gggtgagaaa ttttttacga cgcaagaggc ggaaggcgaa ccaaacgcac atacgacggt
     3181 cgaactgatt gaaaacaggg gggaattcag cctttaccgc cttacgccgc atacgggcaa
     3241 gaaacaccag ttgcgcgtgc atatgatggg tttgggtatg ccgctgttga atgacgcgct
     3301 ctatcccgtg ccgtctgaag cgggcagcga ggattatcgg aaacctttga agctattggc
     3361 aaaaaagatt gcgtttgcag atcctttgtc gggtcgggaa agggtgtttt gcagcggatt
     3421 ttgcctataa tgagagggaa cgcaaacccg tatcggcgga tgccgccgat acgggtttta
     3481 cattatcgat gccgtctgaa agaggtttta tccgttcagg catacgggcg cgaagcggag
     3541 gatggtgtcg cgcaggacga tgagtttgcc gtgaaagaaa tgcgtggaac cggcgatggt
     3601 aatgacgggc aaatcttgcg gttccgccca tttcagtgct ttcccgattt cgacgacttc
     3661 gtcttccgcg ccgtgtatca tcagcgtttt ggcaacattg ggaacggcgg acggctcggg
     3721 gcggccggta tagtggcaga ctgccgcgcc gatgagcagc aataaatcgg gtatgcgcgc
     3781 ttgtgcggca aatgtggcga cataaccgcc gaaggagaag ccggataagg caaattcggg
     3841 ggcttcggga tgttgggcac gggcatagtc gatgacggcg aggcagtctt gcgtttcgcc
     3901 gcgtccgtaa tcgtgtgtgc cttcgctgcc gcctacgccg cgaaggttgg gcaggtagca
     3961 gtggaagccg agtttgctta aggctttggc ggcagtttgg ataactttgt tggtgtttgt
     4021 tccgccctgg agggggttgg gatgattgat gacggcaaca ccgcgtgccg gtacttgttc
     4081 ggacgggata tggatggttt ctaaaatgcc ggcaggaccg gatatgtgta tggtttcggg
     4141 tttcagcata atgatttcct cttgattttc cgcatgttcc gatgcagccg tgaagatagt
     4201 tgcttaaagg tggaaatgct tgccgttgtt ggggttggac gctttcaggc cggcaagcat
     4261 acggtggaag tccaatccgt atgcgttgct cagtttgtcg gcacgttttt tgagtttgtc
     4321 tatgttttgc tctttggggc tgctttggaa ggccattacc gcgacattgc cgtggctttc
     4381 ggcaggaagt tccaagacgc gcccttcaaa aacgctcaac aaccgttcga tgaagcgttg
     4441 gtagcgtttg tcgccgctcc accagttggt tacgaatatg ccgtctgaag agagtgcgtt
     4501 tcggcagtct cggaaaaacg gttcttcaac cagcgcatcg atgatttgtt cgccgtcgaa
     4561 cccgtccacc aaaatcacat cggtgttgtg gcggaagact ttgatatatt ctgcaccgtc
     4621 tgcttcgata atttcaaatt tctcgccctc gaaaggcaac tcgaacaggc tgcgggcaat
     4681 ggcgatgacc tgcgggttga tgtccacggc ggtttggcgc gtgtcgggca ggtaggtgtc
     4741 tatccagcgt gcaaacgaac cgccgcccaa gccgatttgg gtgatatgct gcggaagagc
     4801 gtcggtaaac agcagccatc ccatcatggc acggctgtaa gagagtacca gctcggacgg
     4861 gtggtcgagg ttcattgagc tttggatggt gtcgctgccc aagtgaagcg aacggatatt
     4921 gccttcttcg gaaatgccga cttcgggaaa gccggatttt gcaggacgca ggcggcggta
     4981 gggatgtctt gccatcggtg tgaacggtcg gtttgaaagg cagatatttt atcagaatgc
     5041 cgatggttgt cctgcttcaa attaatttct tttcaaataa attacttatt cggatttgcc
     5101 ggggctttcg gataaattcc ttgccaaggt gcggcattgc ctgcataatt cgctttcttt
     5161 gccggtatag ctcagttggt agagcacctg acttgtaatc agggggtccc gagttcgact
     5221 cttggtgccg gcaccatttc tgctgatggt gttgcaaaac atttcagtaa gccggtatag
     5281 ctcagttggt agagcacctg acttgtaatc agggggtccc gagttcgact cttggtgccg
     5341 gcaccagatt tgacagtccg attgtgtaaa acagtcgggc tgttttgcat ttgcggcacg
     5401 gtcggtggca gggctgcggg catttcacta taatagcccg tttcaaactg aaaaggccgt
     5461 ctgaaaaggg cggggtaaca atatctgatg attactgtga acacactgca aaaaatgaag
     5521 gcggcgggcg aaaaaatcgc tatgctgacc gcttacgaat ccagttttgc cgcactgatg
     5581 gacgatgccg gcgtggaaat gctgctggtc ggcgactctt tgggtatggc ggttcaaggg
     5641 cggaaatcga cgctgcccgt cagcctgcgc gatatgtgct atcacaccga atgtgtagca
     5701 cgcggtgcaa aaaatgcgat gattgtcagc gatttgccgt ttggtgcata tcagcagagt
     5761 aaggagcagg cgtttgccgc cgccgccgaa ctgatggcgg caggagcgca tatggtcaaa
     5821 ctcgaaggcg gcgtgtggat ggcggaaacg actgaatttc tgcaaatgcg cggtattccg
     5881 gtttgcgcgc acatcggtct gacgccgcaa tccgtgtttg cctttggcgg atataaggtt
     5941 caggggcgcg gcggcaaggc gcaggctttg cttaacgatg ccaaggcgca cgatgatgcg
     6001 ggggcggcgg ttgtattgat ggagtgtgtt cctgcggaac tggcaaaaaa ggtaactgaa
     6061 actgttgcct gtccgaccat cggcatcggc gcgggggtcg attgcgacgg gcaggttttg
     6121 gtgatgcacg atatgctcgg tatttttccg ggcaagacgg cgaaattcgt caaaaacttt
     6181 atgcacgggc aaagcagcat tcaggcggcg gttcgggcgt atgttgccga agtcaaagcc
     6241 aaaaccttcc ctgctgcgga acatattttt gcagactgaa caaggtttgc tgctgatgcc
     6301 gtctgaaagc cgtttcagac ggcattttgt tttgccgtgc gcggcgcgta taatcggcgc
     6361 gttttgtcgg gcaggaagcc cgaaagataa ggattaccgt tatgcaaatc atacatacca
     6421 ttcgagaact gcgcgcgtgg cgtaaaaatg cgggaaaggt ggcatttgtg ccgaccatgg
     6481 gcaatctgca tgaagggcat cttgcgcttg tgcgtgaggc gaaaaaacgc gcggacagtg
     6541 tcgtggtcag cattttcgtc aaccgcctgc aattcggtca gggcgaggat ttcgacaaat
     6601 atccgcgtac tttgcaacag gatgcggaca agctggaagc cgagggtgtt gccgttgttt
     6661 tcgcgcccga tgagaaagaa ctctatccga acgtggaaca gcgttacaac gtcgaaccgc
     6721 ccaatctgca aaatgagttg tgcggcaagt ttcgtccggg acatttccgc ggggtggcga
     6781 cggtggtgtc caagctgttc aatattgtcg cgccggatgt tgcctgtttc ggcaaaaaag
     6841 actatcagca gcttgccgtg attaaaggtt tgaccgagga tttgaatttt gatgttgaaa
     6901 tagtgcctgt cgatacgggg cgcgcggaag acggtttggc actctccagc cgcaaccagt
     6961 atttgagtgc ggcggaacgc gacgaagcac cgcgcctgta ctgcgaatta aaggctgttg
     7021 ccgaatcctt ggcgcagggc agtttggatt atgcaggttt ggagcggcag gctgccgacc
     7081 atttgaccgc tgcgggctgg gtggtcgatt atgtcgaaat ccgccgcgcc gatacgctcg
     7141 aagtggcgcg ggcgggagat aagaaactgg tggtcttggc cgccgcccgt ctagggacga
     7201 cgcgcctgat tgacaatttg gaaataaaac tcccttaaac tgcaagcgtc gggaatgccg
     7261 tctgaagcgg atttgcgttt cagacagcat ttattttttt gaacggggtt tcgcaaatct
     7321 acaaacgatt ctgcttgtga taaagttacg cctgattata tgccgtctga aggttcggac
     7381 ggctgtcgga taaaggaaga ttatgttacc taaccgtttc aaaatgttaa ctgtgttgac
     7441 ggcaaccttg attgccggac aggtatctgc cgccggaggc ggtgcggggg atatgaaaca
     7501 gccgaaggaa gtcggaaagg ttttcagaaa gcagcagcgt tacagcgagg aagaaatcaa
     7561 aaacgaacgc gcacggcttg cggcagtggg cgagcgggtt aatcagatat ttacgttgct
     7621 gggaggggaa accgccttgc aaaaggggca ggcgggaacg gctctggcaa cctatatgct
     7681 gatgttggaa cgcacaaaat cccccgaagt cgccgaacgc gccttggaaa tggccgtgtc
     7741 gctgaacgcg tttgaacagg cggaaatgat ttatcagaaa tggcggcaga ttgagcctat
     7801 accgggtaag gcgcaaaaac gggcggggtg gctgcggaac gtgctgaggg aaagaggaaa
     7861 tcagcatctg gacggactgg aagaagtgct ggctcaggcg gacgaaggac agaaccgcag
     7921 ggtgttttta ttgttggcac aagccgccgt gcaacaggac gggttggcgc aaaaagcatc
     7981 gaaagcggtt cgccgcgcgg cgttgaaata tgaacatctg cccgaagcgg cggttgccga
     8041 tgtggtgttc agcgtacagg gacgcaaaaa ggaaaaggca atcggagctt tgcagcgttt
     8101 ggcgaagctc gatacggaaa tattgccccc cactttaatg acgttgcgtc tgactgcacg
     8161 caaatatccc gaaatactcg acggcttttt cgagcagaca gacacccaaa acctttcggc
     8221 cgtctggcag gaaatggaaa ttatgaatct ggtttccctg cacaggctgg atgatgccta
     8281 tgcgcgtttg aacgtgctgt tggaacgcaa tccgaatgca gacctgtata ttcaggcagc
     8341 gatattggcg gcaaaccgaa aagaaggtgc ttccgttatc gacggctacg ccgaaaaggc
     8401 atacggcagg gggacggggg aacagcgggg cagggcggca atgacggcgg cgatgatata
     8461 tgccgaccga agggattaca ccaaagtcag gcagtggttg aaaaaagtgt ccgcgccgga
     8521 atacctgttc gacaaaggtg tgctggcggc tgcggcggct gtcgagttgg acggcggcag
     8581 ggcggctttg cggcagatcg gcagggtgcg gaaacttccc gaacagcagg ggcggtattt
     8641 tacggcagac aatttgtcca aaatacagat gctcgccctg tcgaagctgc ccgataaacg
     8701 ggaggctttg agggggttgg acaagattat cgaaaaaccg cctgccggca gtaatacaga
     8761 gttacaggca gaggcattgg tacagcggtc agttgtttac gatcggcttg gcaagcggaa
     8821 aaaaatgatt tcagatcttg aaagggcgtt caggcttgca cccgataacg ctcagattat
     8881 gaataatctg ggctacagcc tgctgaccga ttccaaacgt ttggacgaag gcttcgccct
     8941 gcttcagacg gcataccaaa tcaacccgga cgataccgct gtcaacgaca gcataggctg
     9001 ggcgtattac ctgaaaggcg acgcggaaag cgcgctgccg tatctgcggt attcgtttga
     9061 aaacgacccc gagcccgaag ttgccgccca tttgggcgaa gtgttgtggg cattgggcga
     9121 acgcgatcag gcggttgacg tatggacgca ggcggcacac cttacgggag acaagaaaat
     9181 atggcgggaa acgctcaaac gtcacggcat cgcattgccc caaccttccc gcaaaccccg
     9241 gaaataatgc aggtccatcc tttcagacgg cataaggttt gccgggaagc tgcggcattc
     9301 gggcaaacgg cacgcagttc gcacgcgttt tgcacggcac accgaatcca ccggccggca
     9361 ggatggcatc cgttaaggaa attctgatga aacacaccgt atccgcatcg gtcatcctgc
     9421 ttttgaccgc ttgcgcgcaa ttacctcaaa ataacgaaaa cctgtggcag ccgtccgaac
     9481 acatcagcag ttttgcagca gaagggcggt tggcagtgaa agcggaaggg aaaggttcgt
     9541 atgcaaattt cgattggaca taccaaccgc ccgtggaaac catcaatatc aatacccctt
     9601 tgggcagtac gctcgggcag ttgtgtcaag acagggacgg cgcattggca gtggacggca
     9661 aaggaaatgt ctatcaggcg gaaagtgcgg aagaattgag caggcagctg gtcggtttca
     9721 aactgccaat ccaatatctg catatctggg cagatggcag gcgtgtggcg ggcgcgcctt
     9781 accgcatcct gccggacggc atattggaac aatacggttg gactgtcggc agaaccgccg
     9841 acagtggggg gcaagtccga acgttgcaac tgaataacgg aaatttgaac atcaggctgg
     9901 ttttcaccga aatcggtatg ccgtctgaaa ccgaaacccc ggaacgctgt gcggcgcgca
     9961 cgagataagg cggacagatg aatattgcgg acggacggca ggcgttttcc gcacctgcaa
    10021 aactgaatct cgatttgagg attaccggca ggcgggaaga cggttatcac aatatcgaaa
    10081 gcatattctg cctgatagat ttgcaggata ccgtatattt gaaaccgagg gacgacggca
    10141 aaatcatcct gcacaatccg gttgatggaa tgccgcagga agtagatttg agctaccgtg
    10201 ccgcatcgtt gctgcaaaaa tatgcgcgca accccgccgg cgtggaaata tggctggaca
    10261 aaaaaatccc gacaggggcg ggtttgggcg gcggaagctc ggatgcggca accgttttgc
    10321 tggtgttgaa ccgttggtgg cagtgcggtc tgacgcagcg gcagctcatt gattcgggcg
    10381 cggctctggg ggcggacgta ccgtttttta ttttcggcaa aaatgcgttt gcgcggggta
    10441 taggcgacag gctggacgaa atggatattc cgaaacagtg gtatgtcatc gtcaaaccgc
    10501 ccgtccacgt ttccactgca aaaattttca cacacgaaag cttgacacga aattccgcct
    10561 caagcataat gccgactttc caaaatctgc aaccgtttag aaatgatatg caggcagttg
    10621 tatttaaaga ataccctgaa gtttggaaag cctattccga gttgtcccga tatggatttg
    10681 ccttaatgac aggttccggt gcgtgtgtat tcacggcgtg tcaagatagg aatagcgcat
    10741 acaatatata ccgacaagtt tcagatttgt acgaggcata tttggcagag ggtctttcaa
    10801 aacatccttt gttgtccgta taaacattgt tggggagtcg tcaagcggtt aagacactgg
    10861 attttgattc cagcatgcga aggttcgaat ccttcctccc cagccaagtc aaacgagttg
    10921 gggagtcgtc aagcggttaa gacactggat tttgattcca gcatgcgaag gttcgaatcc
    10981 ttcctcccca gccagccctt tttggggagt cgtcaagcgg ttaagacact ggattttgat
    11041 tccagcatgc gaaggttcga atccttcctc cccagccaaa taaaagcgtg taagcctgct
    11101 tacacgcttt tatttcatag aaataaaaat attgaaatgc ctttgtttgt gtcggatgtt
    11161 gcaggtataa tgtcgggctt ggtacaagca gagggaagca ttgtgttttc tgagcggaag
    11221 ttaaacataa aatcaggtga gaatatggct gcgtacgaca gtttgatggt atttacaggc
    11281 aatgccaatc ccgaattggc acaacgtgtt gtcaggcatt tggacatttc tttgggcaat
    11341 gcttccgtat ccaagttttc agacggcgaa gttgccgtcg aactgttgga aaacgtacgc
    11401 gggcgcgatg ttttcatcct tcagccgacc tgtgcgccga ccaatgacaa cctgatggaa
    11461 atcctgacga tggcggatgc actgaagcgt gcttcggcag gtcgtattac cacagccatt
    11521 ccgtatttcg gctatgcgcg ccaagaccgc cgtccgcgtt ccgtccgcgt tccgatttct
    11581 gccaaactgg tggcaaatat gctgtattcg gcagggatcg accgtgtttt gactgtcgat
    11641 ttgcatgccg accagattca aggtttcttc gatattccgg tggacaatat ttatgccacc
    11701 ccgattctgt tgaacgacat caaacaacag cggattgaaa atctgaccgt cgtcagcccg
    11761 gacatcggcg gtgtcgtccg cgcccgcgcc gtggcaaaat ccctgaatgc cgacttggca
    11821 atcatcgaca aacgccgccc gaaagccaat gtggcggaag tcatgaacat catcggcgat
    11881 attcaaggca gaacctgtct gattgtggac gatatgattg acactgcaaa tacgctgtgc
    11941 aaagccgccg tcgctctgaa agagcggggg gcggaacgtg tcttggcata tgccagccac
    12001 gccgtattct ccggagaggc agtcagccgt attgcctcat ccgaaatcga ccaagtggtc
    12061 gtaaccgata ccattccttt gtctgaagcg gctaaaaact gcgaccgtat ccgtcaggta
    12121 acgattgccg gtctgttggc cgaaaccgtc cgccgcatta gcaatgaaga atccgtctca
    12181 tatcttttca atgaagaagt gatgacaggc agcatgttgc tgccataagc ccgaagccgt
    12241 cttaagctgg tcgcggccga tgacggcgat tttacctaac ttggagtatt taacatgact
    12301 tatgaaattc aagcctctgt tcgtgaagca caaggcactg gtgcgagccg ccgcctgcgt
    12361 cgcgaaggcc aaatccccgg cattctgtac ggtgaaggtc aagagcctgt tgcaatcgct
    12421 gtggatcaca aaaccgtatt ctacgcattg gaaaaagaat ctttccatac tgcgttgatt
    12481 aagttgtctc tgaacggtga aaccaaagac gttatcgtcc gcgatttcca aatgcacccg
    12541 ttccgccgcg aagttcaaca catcgacttc caagctgtga aagcagacca acccgtacgc
    12601 atccgcgttc ccctgcacat cgttaacgct gaaaattccc aagcggtcaa actgcaaggc
    12661 ggccgcgtat ctctgttaaa cacttctgtt gaagtagttg ctttgcctgc caacatccct
    12721 gctttcttgg atttggattg tgccgaagtg gttgccggcg acattctgca cttgtcagac
    12781 atcaaactgc ctgaaggtgt agaaagcgtt tccctgaaac gtaacgaaaa tctggctgtt
    12841 gctaccgtta ccggtaagaa acgctaattg atttcagcag cagggcggcg cgtatgcaat
    12901 acgtaccgcc ctgttgtttt atgccgtctg aaccgtgttt cagacggcat ttctttattt
    12961 gttggaaaaa cgggatattt gaaacggcag attactgccc tgtcagacac gcccaaagcc
    13021 tttgccaccg gcttcttttt tttacatttt ccagtgcgac gatttctttt tcggcaatgg
    13081 tgtatccgtt ttgcctgatt ttgatttttc ctaaaatctg cccttttttt accggggcgg
    13141 gaatcggctg tatggtttct agaatttgtt ctgccatttt cgcttcctta tgtggcagag
    13201 tgatgtaggc ttctttgagg aagcctgcgc ggacggtttt tttgctgcct ccggaaattt
    13261 ggatttgggc aacggttttg cctttcggat atattttggg cgtatcgaag gcctgcaatg
    13321 cccggttcag cagcttgctg ttgtctgatg cgcgtgtttc cgccgattcc gaacccaatg
    13381 tgatgacaag gatgtgcctg ccgttgccgg agtatgacac ggcaaggttg tagccgccgc
    13441 tttctgtgtg tccggctttc agaccgttta cattgttgtc cctatataaa aggatattgc
    13501 ggttgttttg ctctatattt ttgaatttga aagatttgat ggaaaacagc gggtaatatt
    13561 ccggaaagtc gcgcatcaat gcttcagaca gctgggcgag gtctttggcg gtggaaacct
    13621 gtccttctct actcaagcct gtcgggtttt tgaatacagt gttcttcatg cccaagcgtc
    13681 gggcttcttt gttcatttgt tgcacaaaat tttcaatcga gccgttgccc agccggccgg
    13741 caagggttag ggcggcatcg tttgcggata gtgcaatcat gccttttaag agtttgtcgg
    13801 tgctgaccgt atcgccggga cgtacaaaca ttctgcttcc ttctgaagcc catgcggatt
    13861 cgggtatttt taagttttct tcagattgga tatttcccga tttcatgttt ttgaaaacca
    13921 aatatgcggt catcagttgg gttagtgccg ccggttcgac aggggtattg atgtttttgg
    13981 cggataaagt ctgtctgctt tgaaggtcga taacgatgtg tgccgctgtg agggtttcgg
    14041 gtgtttggaa cgtgggggcg gcgtgtaccg ccggtttgtt gggcgcgggc gatgcggccg
    14101 ttgcgtgaga aacgcctaag atgatggaaa gcaggacggg caggatttta tgtgctgtca
    14161 tgaaatattc taattgtgtg cgtgtttcag tctgccgatt atacgcttag ggtgtctgat
    14221 cgggcggatt tttcttgctt tcgcgccgtc ttgggcgtat ggttttgggt tttgcgattt
    14281 taataaaccg attatcccat attgaattat gaacacgccc cttccttatt ccaattacct
    14341 catccgcatc ctgacggcat ctgtctatga tgtcgcggtc gaaacgcctt tggaaccggc
    14401 acgcagcctt tctgtacgtt tgaaaaacaa catccttttg aaacgcgaag atttgcagcc
    14461 ggttttttcg ttcaaaatac gcggcgcgta caacaaaatg tccaagttgc cgaaagatgc
    14521 gctcgcttgc ggcgtgattg cggcaagcgc gggcaatcat gctcaaggcg tggcattgtc
    14581 cgcacagcgt ttgggctgcc gtgccgttat cgttatgccg gagactacgc cgaaaatcaa
    14641 agtggatgcg gttaagagcc gtggcggcga ggtggttttg cggggcgttt catacaacga
    14701 tgcctacgat tatgcgatgg agttggcgga aaaagaaggg ttaacctata tcgcgccgtt
    14761 tgatgatcct gatgtgattg cgggacaggg gacggtgggg atggaaattg tcagccagca
    14821 tcccgatcca atccgcgccg tattcgtacc gataggcggt ggcggtttgg cggcgggcgt
    14881 ggcggcattt atcaagcagg tccgtcccga aatcaaagtt atcggcgttc agaccaacga
    14941 ttcctgctgt atgaagcagt cggtcgaagc gggtgaaatc gtccatttga aagatgtcgg
    15001 gctgttttca gacggcactg cggtcaaagt cgtcggaaac gaaaccttcc gcctctgcaa
    15061 agaacttttg gatgaaatca ttacagtcga taccgatgcg gtttgcggcg cggtcaagga
    15121 tattttcgat gacacgcgca gcattaccga gccggcgggc gcgttggcgt tggcgggtct
    15181 gaaagcctat atcgcccgag aaggcgcgga aaaccaaacc ctgattgccg ttaccagcgg
    15241 tgcgaatatg aattttcacc gtttgcgcca tgtgtcggag cgtagtgaat tgggcgaggg
    15301 caacgaaggt atttttgccg ttaccatccc cgaagaacgc ggcagcttcc ttaagtttgt
    15361 caatatattg ggaaatagga atattaccga gttcaactac cgctacggag acgatgaaaa
    15421 agcgcatatc tttgtcggac ttcaagcggc aggtccgcag gatttggcgg ttatcggtag
    15481 ccggttggat gaggcgggat tgcccaatgt cgacctgacc gatgatgaga ttgccaaaat
    15541 ccatatccgc tatatggtcg gagggcggac ggacaaagta gaaaacgagc gtctggtcag
    15601 ttttgagttt ccggagcgtc cgggggcgtt ggcgcgtttt ttgaaccata tgcagggcgg
    15661 ttggaacatt acgctcttcc attaccgcaa ccacggtgcg gattacgggc ggattttggt
    15721 cggtatcgat gtgccgccgc acgatgccgc cgcatttgac ggtttcttgg aaagtctggg
    15781 atacagctat cacgaggaaa cgcaaaatgc cgcgtacaag ctgtttcttg cctgacgctt
    15841 gaaagcacaa tgccgtctga aagcctttca gacggcattg cgctttcatg gttaaatcga
    15901 atattcaatc agttcgtttt gagagaagac ataaacctgt ttcggaatca gcttcaattc
    15961 tttgccttcg gcgattgggt aacgcgcggc atcgctgcca gccagcgtga tatgcacgtc
    16021 ttgtttgccg tgtttcacca aaatatgcgt caatgccccg acggcgtgga ctttttcgat
    16081 ttcggcacaa atcatcggtg tttcgtgttc ggcggcgatc tgccattcgt gcgggcggat
    16141 atagccggtg gcggtttgtt cctgccattt gtattgtgcg tccaatttcc acgcgaagcc
    16201 gttataatgc cagaagcctt tttcgatacg tccttcaaaa gcgtcggttt cgccgaggaa
    16261 ctcggtaacg aaggcatttt cgggtttgcg gtaaatagct tcggcgctgc cggtttgttc
    16321 gattttgccg tggttcatca cgacgatttc gtcggaaact tcgagggctt cttcttggtc
    16381 gtgcgtaacc agaatgctgg ttacacccag gttgtgatgg atgtcgcgca gccaggtgcg
    16441 taattctttg cgtactttgg cgtccaacgc gccgaagggt tcgtccaaaa gcaggagttt
    16501 cggttcgacc gcaagcgcac gggcgagggc gatgcgctgg cgttgcccgc cggagagttg
    16561 gtgcggatag gattttgcca aatgcgagag ttgtacgagt ttcagcaatt cttcgacttt
    16621 agtgcagatt tgttctttgg acgggcgttc ggacttgggc aatacggtca aaccgaaagc
    16681 gacgttgtca aacacgttca tgtggcggaa gagggcgtag tgttggaaca cgaagccgac
    16741 tttgcgctcg cggacgtgtt tggcggttac gtcttgccca tcaaacagaa tcttgccgcc
    16801 gtcggcgttt tccagtccgg cgataatgcg taaaagcgtg gttttgccgc agccggacgg
    16861 gccgagcagg gaaacgagtt tgccggtggg gacgttgagg ttgatgtttt tcagcgcgtg
    16921 aaaattgccg aagtgtttgt ttaagttttg gatggtgata ctcatactgc gttcctttcg
    16981 gcggcggcga gttttttgtc ttgtaatttg gtgatgatgt tctgcaccgc cagcgtcgcc
    17041 agtgccaaaa gtgccaatac gccggagagg gcgaatgcgc cggtgaagtt gtattcgttg
    17101 tagaagattt cgaccaaaag cgggacggtg ttggtttcgc cgcgtatgtg tcccgatacc
    17161 acgctgaccg cgccgaactc gcccatcgcg cgggcgttgg tgaggatgat gccgtagagt
    17221 aacgcccatt tgatgttggg cagggtaacg cgccaaaaca tctgccagcc gcttgcgccg
    17281 agtatcaatg ccgcctgttc ttcgctgtcg ccctgagcct gcatcagcgg gatgatttcg
    17341 cgcgcgacaa aggggaaggt aacgaacagc gtcgccagaa taatgccggg gatggcgaag
    17401 ataatctgta tgccttgcgc ttcgagccag ccacccaatg ccgtgtgcgc gccgaacaat
    17461 aagacgaaca ttaaaccggc caccacgggc gatacggaaa acggcaaatc gagcagggta
    17521 gtcagcaact gcttgccgcg aaaatcaaaa cgggtcagca gccacgccat cgccacgccc
    17581 aagacggcat tgacggggac gacaatcagc gcggtaatca gcgtcaattt gatggcagac
    17641 cacgcttcgg gatcgtttaa ggatttcagg tacaaatccc aaccgccttt taaggcttcg
    17701 taaaatacgg cgacgagcgg cacgaccagc atcagcagca gaaagcccag cgcggcggca
    17761 atcagcaaca cgcgcagccg acgcggttcg gtcaggttgg gattggcgga atagggtttc
    17821 atggcggtgg tgttctctgg ttggttttaa aatgtttgga ggtcgtctga aaaactgtgt
    17881 ttttgggagc ggattcgttt tcagacaacc ttttattgat taagggaata aaaacgtcta
    17941 gctaccgtca ttcccgcgca ggcgggaatc caccacaggg caacaggaaa acagaaaata
    18001 aataaggcag ccgaaattca ccaatggatt cccgcctgcg cgggaatgac ggtaacaggt
    18061 atttcagacg acctcaaccc ttcgcgcccg aacgcctgcc caacgcccac tgcatcacgt
    18121 tcagcgcaaa cagaatcaca aacgaaacca gcagcataaa caacgccacc gccgacgcgc
    18181 cctgtacgtc gaactgttcc agcttgcccg taataatcag cggcaggatt tcagaaacca
    18241 tcggaatatt gcccgcgata aaaatcaccg agccgtattc ccccgttgcc cgcgcgaaca
    18301 tcattcccgc gccagtcaag agtgccggcg tgatttccgg cagcagcacg cggcggaacg
    18361 tcgtaaagcg gtttgcgccc aaagtcgccg ccgcttcctc atattcgccc gataattcct
    18421 ccaacaccgg ctgcacggcg cggacgataa agggcaggct gacgacgacc agcgcaatcc
    18481 aaatgccgac gggcgtaaac gcgattttga tgcccaaagg ctcgaaaaaa cggcctatcc
    18541 aaccgttggg cgcatacagg gtcgccaacg cgatacccgt aaccgccgtc ggcagcgcaa
    18601 acggcaaatc gaccagcgcg ttcgccagac ccttgcccgg gaattcataa cgcaccaata
    18661 cccacgccac cagcgtgccg aacacgacat tggtcagcat cgcataaaac gacatccgca
    18721 aactcagcca gacggccgcc aagacattgg gttcggcaat cgtgttccaa aagccgcccc
    18781 agccgatttc cgccgccttc gccgccatca tcgcaaacgg caagaccaca agcagcgaca
    18841 ggcacaatac ggtcagacca aggctgagtt tgaagccggg cagtacgccg ggcgttttga
    18901 gcggtaacat aaaacaatgc tgaaaataag gaaaaggaag gactacttta acgatgccgt
    18961 ccgaaaaacg gaaagaatgg aaagtttggt gcaaagacga atttgttata aagcggttgg
    19021 cagtttctca agcgggcgcg atgttttaaa atatagtgga ttaacaaaaa tcaggacaag
    19081 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt
    19141 aaatttaatc cactataaca ccttgttttg acggaaaacc atcatataaa ggaacactta
    19201 tgcagatttt atcttttcaa ccggacattg cggaacgtat gctggaaggt acggaaggcg
    19261 agtcggtcaa cgaaaacgca caattcgtcc gtacggacaa cggctattgg attgcgtggc
    19321 atgaaggcgt agcggcactg cttgcgcccg atacgccgcc gggcattccc tgtttttggg
    19381 tggaaggcac ggaaagcctt gaagagttgt gcgtcatggt ggaacgcggc gagtttgacg
    19441 aagtggaaga gtttgacggc gatgacgacg aatggctcga aacggcacag ggttgcgggc
    19501 accacggcga agcttgcgcc tgcggacatt aaaggcattg caggcttgcc gcaaggggtg
    19561 caaggctttg ccgtttttta aataatcggt ggattgcatc tgtttgcaat gggcggaacg
    19621 cttccctttg tattttattg aataaaaaaa cttatcttgg tattataatt aaggcatcat
    19681 caattatttt gggatggcaa taaacgcaaa gcattgattt gcgccgattg cagacttatt
    19741 atagcaggtt gcggcgcgga cttaatggga attgtttata gagcatgatg gtctgaaaca
    19801 tcattaatta aaaataccat taatccgatt tatatttatt tcaatttcaa tggaaaaaca
    19861 tcaatgacaa tgattttaag cattttaagc ctgtttttta tcatcagact gttattttta
    19921 gccgtctcta ttaaacatga aaaagccttg attgccaaag gggcgaaaca atacggaaaa
    19981 accaattcca cgctgcttgc ggcagttcat acgctttatt atttggcgtg ttttgtttgg
    20041 gtatggcttt ctgacactgc ttttaatggc atatccttga ttggtacgct gacggtgatg
    20101 gcttcgtttg tgatattgtc attgattatt aagcagttgg gggagatttg gacggttaaa
    20161 atctatattt taccaaatca tcaaattaat cgttcgtggt tgtttaaaac attccgccac
    20221 cccaattatt ttttaaacat catacccgaa ctgattggca tcgccttatt atgtcaagcg
    20281 tggtatgttt tattgattgg cctgccgatt tatttgctgg tcttatttaa gcgtatccga
    20341 caagaagaac aggcgatggc aacacttttt taacccgttt catcaattat agcggattaa
    20401 caaaaaccag tacggcgttg cctcgcc
//
LOCUS       DAWHXS010000030        20159 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWHXS010000030 DAWHXS010000000
VERSION     DAWHXS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.030"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(183..2972)
                     /gene="ileS"
                     /locus_tag="WIE22_000830"
     CDS             complement(183..2972)
                     /gene="ileS"
                     /locus_tag="WIE22_000830"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ4126945.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDRPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETLRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTHDVGAVAGHETICKR
                     CAGNVGGEGETRHYA"
     gene            complement(3113..4033)
                     /gene="ribF"
                     /locus_tag="WIE22_000831"
     CDS             complement(3113..4033)
                     /gene="ribF"
                     /locus_tag="WIE22_000831"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ4126946.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            complement(4332..5627)
                     /gene="tyrS"
                     /locus_tag="WIE22_000832"
     CDS             complement(4332..5627)
                     /gene="tyrS"
                     /locus_tag="WIE22_000832"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ4126947.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEVLVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5690..7558)
                     /locus_tag="WIE22_000833"
     CDS             complement(5690..7558)
                     /locus_tag="WIE22_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ4126948.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTTILHREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSLRVQFGFLAALCALSLAASFIPSALDKYYLPHLRACELLIGSLTAVWMRCRQPAV
                     GRRCAAVGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKF
                     FQSKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAVVLILLLSLFSYHCI
                     EKPFKKWQGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNT
                     GKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASR
                     VFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHK
                     LSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAK
                     YPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(7569..7673)
                     /locus_tag="WIE22_000834"
                     /pseudo
     CDS             complement(7569..7673)
                     /locus_tag="WIE22_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7925..9016)
                     /gene="ychF"
                     /locus_tag="WIE22_000835"
     CDS             complement(7925..9016)
                     /gene="ychF"
                     /locus_tag="WIE22_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ4126949.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9129..10805)
                     /locus_tag="WIE22_000836"
     CDS             complement(9129..10805)
                     /locus_tag="WIE22_000836"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ4126950.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            10980..11411
                     /locus_tag="WIE22_000837"
     CDS             10980..11411
                     /locus_tag="WIE22_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ4126951.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11642..12337)
                     /locus_tag="WIE22_000838"
     CDS             complement(11642..12337)
                     /locus_tag="WIE22_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ4126952.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDAPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12355..12855)
                     /gene="hpaC"
                     /locus_tag="WIE22_000839"
     CDS             complement(12355..12855)
                     /gene="hpaC"
                     /locus_tag="WIE22_000839"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ4126953.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            complement(12970..13410)
                     /gene="nadR"
                     /locus_tag="WIE22_000840"
     CDS             complement(12970..13410)
                     /gene="nadR"
                     /locus_tag="WIE22_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ4126954.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(13753..14262)
                     /locus_tag="WIE22_000841"
     CDS             complement(13753..14262)
                     /locus_tag="WIE22_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ4126955.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14342..14417)
                     /locus_tag="WIE22_000842"
     tRNA            complement(14342..14417)
                     /locus_tag="WIE22_000842"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14382..14384),aa:Lys,seq:ttt)
     gene            14628..15707
                     /gene="apbC"
                     /locus_tag="WIE22_000843"
     CDS             14628..15707
                     /gene="apbC"
                     /locus_tag="WIE22_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ4126956.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDFAARLNVPLLGQLPLSLPVREAMDGGAAAQLFDEHPAIARIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
     gene            16964..20083
                     /locus_tag="WIE22_000844"
     CDS             16964..20083
                     /locus_tag="WIE22_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ4126957.1"
                     /translation="MNKTLKRRVFRHTALYAAILMFSHTGGGGAMAQTYKYAIIMNEG
                     NQLEVKGNGQYSTIKDKDREREYTYHQGRGGGGSVSFNNSDELVSRQSGTAVFGTATY
                     LPPYGKVSGFDERKLKERTNALNWIHTTHPGLIGYSYAGVVCRDSTGCPKLVYKTQFT
                     FGNSGLAKKANGGGLDIYVDKSRDNSPIYKLKDHPWLGVSFNLGSENTVKNSKSSNKL
                     ISSFSEDNNNQTIVSTTEGSPISLGDQQREHTAVVYYLNAKLHLLDKKQIQNITDKTV
                     QLGVLKPSIDVRQQKGANWLSFWASWDIKDTGQIPVKLGLQQVKAGRCINKPNPNPNK
                     KDLSPALTAPALWFGPVKDGKAEMYSASVSTYPDSSSSRIFLQNLERKTDPGRPGRHS
                     LKPLSDTQIKSREPNFTGRQTVIRLDKGVHQIKLKGNEVEGFKGNNGNDTFGIVREGS
                     FTPDASEWKKVLLPWTVRDSNDDGRFNAVNKEENNGKPKYSQRYRIRDNNGNRNLGDI
                     VNSPIVAVGEYLATSANDGMVHIFKKNGGGDDRNYSLKLSYIPGTMPRKDIENKESTL
                     AKELRAFAEKSYVGDRYGVDGGFVLRRITDDQDKQKHFFMFGAMGLGGRGAYALDLSK
                     IDSNNPTAVSLFDVKHDNSGNNSNNSNNSNNSVQLGYTVGTPQIGKTHNGKYAAFLAS
                     GYATKTIDSTDNKTALYVYDLESNNGTPIAKIEVTGGKGGLSSPTLVDKDLDGTIDIA
                     YAGDRGGSMYRFDLSSQDPKQWSARAIFKGDKPITSAPAISQLKDKRVVIFGTGSDLS
                     EDDVLSTDEQHIYGIFDNDTNTGTAQDGQGNGLLEQVLKKDGNTLFLSDYKRSNGSGD
                     KGWVVKLEAGQRVTVKPTVVLRTAFVTIHKYTGNDKCGAETAILGINTADGGKLTKKS
                     ARPIVPAANSKVAQYSGDKKTSSGKSIPIGCMEKDNGIVCPNGYVYDKPVNVRYLDEK
                     KTDDFPVTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLLMNDLDSLDITGPMCGMK
                     RISWREVFF"
BASE COUNT         4793 a         5412 c         5417 g         4537 t
ORIGIN      
        1 atcggaaatg actgaaacct cgagattcta gattcccact ttcgtgggaa tgacggttca
       61 gttgcattcc gacaacaccg caatcttgaa acccctccgc cgttataaag acaaatcgcg
      121 gtacaaaaaa tgccgtctga aatgctgttt ggcggtttca gacggcattt gctcaaactt
      181 tatcaggcgt aatggcgcgt ttcgccttct ccgccgacat tcccggcgca gcgtttgcag
      241 atggtttcat ggcctgcaac tgcgcccaca tcgtgggtgt agtgccaaca gcgttcgcat
      301 ttttcaccat cgctggcttt ggcggcaacg gcaagttcat cacctacttt cacttcggct
      361 ttagacacca gcagggcaaa gcgcaattct tcgcccaaag cgttcaggta gctggccatt
      421 tcttccggcg cagtgatttc ggcttccgcc tgcaaggacg aaccaacggt tttgtcggcg
      481 cgcaaaggct caatggcggc ggttaccgct tcgcgggctt cgcggattgc cgtccatttt
      541 ttcaccagtt cggcttcggc tttttcgttg atggtcggga actcgtgcca agtatggaaa
      601 aggacgctgt cttcttcgcc gccgccgatg atgtcccacg cttcctcgcc ggtgaagcac
      661 aaaatcggtg caatcaagag aaccaaactg cgtgtgatgt gatacagggc agtttgtgcg
      721 ctgcggcgtg catggctgtc tgctttggtg gtatagaggc ggtctttcag gatgtcgagg
      781 tagaacgcgc ccaagtcttc cgagcagaaa gaaacaatgt cttttacggc gaagtggaag
      841 gcgtagcgcg gatagtaatc gcctgccaga cgctcttgca gccgacgcgc caacaccagg
      901 gcatagcggt cgatttccac catatccgcc tgttgcacgg catcttcaat cggattaaag
      961 tcgctcaagt tggcaaacaa aaagctcaag gtattgcgga tacggcggta gctttcggtt
     1021 acgcgcttaa ggatttcttt agaaatcgcc aattcgccgc tgtaatcggt agatgccgcc
     1081 cacagacgca ggatgtccgc gccgaattcg ttatagactt cttgcggcgc gacgacgttg
     1141 ccgatggatt tcgacatttt tttgccttcg ccgtctacaa caaaaccgtg ggtcagcagc
     1201 tgtttatacg gtgcgcgtcc cattgatgag gcgcagccgg tcagcatgga cgattgaaac
     1261 cagccccggt gctggtcgct gccttcgaga tacaaatccg ccggccattc caattcttcg
     1321 cgttgtttca caacggaata atgggtcgaa ccggaatcga accatacgtc catcgtatcg
     1381 gggagtttgt cgtaatgttc gcaatcttcc gcgcttaata attcgctttt atcgagggag
     1441 aaccaagcct cgatgccttt ttcttcgatt ttcagggcaa ctttttccaa aagttcggca
     1501 gagttcggat gcagctcgcc cgtttctttg tgaacaaaga aagtcatcgg cgtgccccaa
     1561 tagcgttggc gtgaaaccac ccagtcagga cgaccttcaa tcatggcttc caaacgcgcg
     1621 cgaccccaag acgggaagaa ttcggtgtcg tccacggcct tgatggcttt gtcgcgcagg
     1681 gttttgccgt cggcaccggc tttgtccata ccgacaaacc attgaccggt cgcgcggtaa
     1741 atcagcggcg ttttgtgccg ccagcagtgg gcgtagctgt gttcgatttt actgcttgcc
     1801 aaaaggttgc cggtttcttc caaccattgc aggatgacgg ggttcgcctc ccaaacgcgc
     1861 ataccggcaa cgcgcagcgt ttcgccgatg tatttgcctt cggcgttgac aggattgtaa
     1921 agctcgatgc cgtatttgtt gcagacggcg tagtcttcca aaccgtgcgc gggcgcagta
     1981 tgcaccaagc cggtaccggc atcggtggta acgtgttcgc cgttaagcat gggaatatcg
     2041 cgttcgagga acggatggtt catgtgcagg ttttccagct tgtcgccggt ggtttcggca
     2101 agaatagaaa tgccgtctga aaagccgtag cgtttcaaag cgtcttccgc caaatctttg
     2161 gccaatacca atttgccttt gggcgtatcg attaattgat acaccacgtc tgcacccgct
     2221 gaaacagctt ggctcgcagg caaagtccaa ggcgtggttg tccaaataac ggcaaacgct
     2281 ttgccttcga tacctgccaa accgaatgcg gcggcaagcg cggcggtgtc tttaaacgga
     2341 taggcaacgt caatcgcggg cgagattttg tctttatatt ccacttccgc ttcggccagc
     2401 gaagaaccgc agtccaagca gaattgaacc ggtttcgcac cccggtagag atagccggat
     2461 ttgtagattt cgccgagcat acgcacggta tcggcttcgg ttttgaaatc catggtcagg
     2521 taaggacggt cccaatcgcc caacacgccc aagcggataa agtctttttt ctggcgggca
     2581 acttgttcgg cggcatattc gcggcacaat tcgcggaaac gcgctttggg catgtctttg
     2641 ccgtgcagtt tttctaccat cacttcgatg ggcaggccgt ggcagtccca acccggcaca
     2701 taaggcgcgt caaaaccggc ttgggttttg ctgcggataa tgatgtcttt gagaatttta
     2761 ttgacggcat gaccgatgtg gatgtcgccg ttggcatacg gcgggccgtc gtgcaggatg
     2821 aatttcggac ggcctttggc gatttcgcgc agtttttggt agcgtttttg ctcgtaccag
     2881 cttttcagcc atgcaggctc gcgcttggca agattgccgc gcatcgggaa cgggctttcg
     2941 agcaggttga cggttttgct gtaatcggtc attttttaat ctctattgtt acaatatttc
     3001 ggttcagacg gcattgcgcc tcaaacagta tttcacaacg ggaaacccct atgccgtctg
     3061 aacagttaaa agattgattg tagcccaatc ggatggtttg tataaggttt ttctaccaac
     3121 actttgcggc ttccatatcg gctgctatct gccttttcag ttctgccata ccgtcaaact
     3181 tttcttcatc gcgcagtttg tgcaggaagc ggacgttcag ccgttgtccg tacaggtcgc
     3241 cttgaaagtc gaacaggtgg acttcaagct tttgagaaca gccgctatca acggtgggat
     3301 tgaaaccgaa gctcgccacg ccgcgccgcg tgccgaatgc gccgtccgct tcgacgacga
     3361 acacgccgcc gagtgcataa cggtggcggg gcaggcggat gttggcagtc ggggcgttta
     3421 aggtgcgtcc gagttttctg ccgtgcacca ccctgccgct caagacgtag tcgtgtccca
     3481 aaagtttttt cgcataggca aggttgccgt ctgaaagggc ttgtcgcacg gcggtactgc
     3541 tggtgcggat gtcttcgacg atgacggaag gcgtacgctc ggtctgcata tcgggctgtt
     3601 gtgccaaaag ttcaaaacag ccttcccgcc ccgcaccgaa acggaaatca tcgccgacga
     3661 gcaaataacg cgtattcaag gtttgacgca gcaggcggtc gataaaccct tgcgcggata
     3721 tttcggaaaa attttgatcg aaacgcaaaa cccagacggc atcgacacag cctgtgcctt
     3781 ccaataattc gagcttggtg cgcagggggc tgatccgaca cggtggcatc ctgccggtgc
     3841 ggagtgcgaa aaattctttg ggttggggtt cgaaaacgac ggtcacgacg ggtaatccgc
     3901 gcgtatcggc ttcgaggcgg agtttttgga ggatgtgttt gtgtccaagg tgtacgccgt
     3961 cgaaattgcc tatggttacg gcggccccgt gcggaaagtc gggcgcgttg tgccgcccca
     4021 gcctgattct cattgttgcg ttcgggtatg tggtgaaaca ggcggtcatt gtaaacggta
     4081 ttgcggttta tagacagtgt gccgcccgtt acgcccgccc gacgctggaa aagcaggcaa
     4141 atttcccgcc gccgaacgcg ccaaacgcat aaagcgtgca tcaggcgcga tcctatgtgt
     4201 tgaaacatcg ccccaaacct gatgcaacat cagtgaaaat cgttcttttt taaccaggta
     4261 aaccgagaca aacaaccctc cgccgtcatt cccgcgcagg cgggaatgac ggcatttctg
     4321 cggcaatcgg attatttcca aaccaaaagc gcgtggttgc gtttgccgcg ccgaaggatg
     4381 gtgtatttgc cgaaacgttt gtgttcgtcg gtcagcaggc aggcatcgtc ggggcgttcg
     4441 gcggcgtggt tggggttgtt ggcttcggca ggtttgccgt tgagcaaaac cgctttgctg
     4501 ttcacaaagc cgcgcgcttc tttattggag gatgccaaac cggtttttac caagacttcg
     4561 acgacattga tgccgtctga aacttcaaat gcaggcagac cgtcgagggc gagctgctcg
     4621 aagtcgcttt cggtcaggct gctttggtct tcggcaaaca ggctttcgga aatgcgttgc
     4681 gcggcggcaa gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt
     4741 tgcgcttcgg gcttgctgcc gcttgccttg tctttggctt cgatggcatc gatttcttcg
     4801 atggacagga aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc
     4861 cagaattggt agaactgata gggcgaggtt tttttcgcgt tcagccatac cgcgccgcct
     4921 tcggttttgc cgaatttggt accgtctgat ttggttacca aaggcagggt cagaccgaat
     4981 acttgttttt ggtgcaggcg gcgggtcagg tcgataccgg cggtgatatt gccccattgg
     5041 tcggagccgc cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtaa
     5101 ccttgcagca gggaataggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcgatg
     5161 cgctgtttga cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg
     5221 cgcaggaagt caaggcagtt catgctgccg aaccagtcgg cattgttcgc cataatggcg
     5281 gcatttccgc cttcaaagct caagaaaggg gttaattggt tgcggatact ttccacccag
     5341 ccggcaacag tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg
     5401 atcataccgg tcgcgccgcc caccaaagca atcggcgtat gccccgcctg ttggaagcgg
     5461 cgcaatgcca atacgggcag caggtgtccg atgtgcaggc tgtcggcggt cgggtcgaag
     5521 ccgcaataaa gggcaatttt ttgttcgttc aacaaagcgt ctaaggcttc gatgtcggtg
     5581 gtttgcgcga taaggccgcg cgattgcagg tcttggatga cgctcatcgg tctctttcaa
     5641 aaaaaattag cgtttttgca aaccgccgat tgtaacaaat ttaagcgaat caatggttat
     5701 ggcgcgtatc gagaaaccgt tgtttttcgg aaaaacgctt tgccaattcc gtgccgccgt
     5761 aagggttgat gtggtctttg tccgagtaaa ccggcaatcc gccgatttga aaatctgcgg
     5821 ggatataggc ggcggcatca ataatataga cgttggggta tttggctgcc aattccctga
     5881 tgcgtgcatt ggctttcagg gtgctttcgt cgtccgggcg cagggcttgg cggtaacccg
     5941 gtatgcgtga agacaagata taggcgcgct ggacgttgta agacgaggca aggttgtccg
     6001 ccatcaggta aacggcttgt ttttcggacg agagtttatg cagcatacgg tcgaattttt
     6061 ggaaaaaacc ggcatcatag gcaagggagc ggctgttttc gggcatttgg ctgccccagc
     6121 gcatcgccaa aaccactttt gaataccggg gcaggtgttc ttcggcatag cgataaacgg
     6181 cgcggcaggc tgcccagttt tggaacacac gggacgcgta gccttccaca taggcgcaag
     6241 cgtcggcgga aaccatagtg gcggaccatt tttctttttt gcccacggca tcgaagaatg
     6301 ttttgtaatg gtcggcgtgg gagtcgccca aaaccagcag ttccggctgt ttttccgtat
     6361 ccccccatag gcattgtttg ccggtattgt tgtggcagga ggtgttggaa cgcgtcagcc
     6421 ccaagcggtc gtattgcgcc ataaacggca gtctcatcgc aaaaaacgag cccgccccca
     6481 aaatgagcat aggcaaggca taaatccata aaacggattg tgcgaacgaa ccttgccatt
     6541 ttttaaacgg tttttcgatg cagtggtaag aaaacaggga aagcagcaat atcaggacga
     6601 ccgccgccgc cggcgaataa ggcggcaggt tgtccgggcc gatatagcgc ataaaggcca
     6661 atatcggcca atgccacaga taaagcgaat aggaaatcaa accggcggca acagtgattt
     6721 tcgattggaa aaatttttta agcgggtgtt cgtaatgatt gaaataaatc agcgcggcaa
     6781 cagccagaca gggaatcaaa gcggcggggc ccgggaaata ggcggtttgt tccgaatagg
     6841 aaaacaggca ggttgacaat atgcacacgg caaacaatgc gccgacggcg gcacagcgtc
     6901 tgccgacggc aggttgccgg cagcgcatcc acacggcggt cagcgatcct atcagtaatt
     6961 cgcaggcgcg caggtggggc aggtaatatt tatcgagcgc ggaaggtata aaggaggcgg
     7021 caaggcttaa ggcacacagt gcggcaagga agccgaactg tacgcgcagg cttttgcggg
     7081 cgacaagcag cagcagtatc ggaaagacaa agtaaaattg ttcttcgacc gacaaagacc
     7141 agatgtgcag caggggcttt tcttcctgcg cgggatcgaa ataatccttc ccccttgcaa
     7201 aatacaggtt agaggcgaaa cccaaggcgg tcagcgcgga tttccacaaa agaaagaaat
     7261 catctttggt gaataaaaag aagccgcctg ccagcgttgc cgccaatacg gcgaaaaatg
     7321 cgggcagaat ccgcttgatg cggcggatat aaaatgcctt cagggaaaac ctcccccccc
     7381 ccgacatttc gcggtgaaga atcgtcgtca tcaaaaagcc tgaaatcaca aagaatatat
     7441 cgacaccgag aaacccgccc ggcagccaat ccttttcgat atggaacacg atgacggaca
     7501 agacggcggc ggcgcgcaat gtgtcgatgt ccgggcggta gggtaaggct tggctcataa
     7561 tgtttttata gtggattaaa tttaaaccag tacggcgttg cctcgccttg ccgtactatc
     7621 tgtactgtct gcggtttcgt cgccttgtcc tgatttaaag ttaatccact atactcgaaa
     7681 cgcggcggcg caaatgccgt ctgaaaggtc attttgtacg ggatattcgg aatgccggac
     7741 ggatttttgc aacagcgggg caggcggttt gttttgcagg aatcggggag ggcaaatcgg
     7801 aaatgcggac gggattttat tttgatgcag ccgcattccg gcggtatgga aacgccgaaa
     7861 accaccaaaa ccggcttcag acggcatttc tggcaaagcc gtctgaaaac ctgccgcatt
     7921 tgggttacac gttaaacaaa aagtgcatca catccccatc ctgcacgacg tattccttac
     7981 cttccacacg cattttgccg gcttctttgg ctttggcttc gccgccgagc gagacaaagt
     8041 cgtcgtaaga aatgacttgg gcgcggatga agccgcgttc gaagtcggtg tgaatcacgc
     8101 cggcggcttg tggggctgtg tcgcctttgt gtatcgtcca cgcgcggact tctttgacgc
     8161 cggcagtgaa gtaggtttgc agccccaaaa ggtcgtaacc ggcgcggatc aggcggttca
     8221 ggcccggttc ttccaagccc atttcggcga ggaactcggc tttttcaccg tcttccaact
     8281 cggcgatttc gctctccatc gcggcgcaca cggcgacgac gggggcgttt tcttttgccg
     8341 ccaattcttt caggcggtcg aggtgcgggt tgttttcaaa accgtcttcg gaaacgttgc
     8401 cgacgtacat ggcaggtttg gcggtcagca ggaacagcgg tttgagcatc gcgcgttctt
     8461 ccgcgtccaa accgaaggaa cgcacgggtt tgccttcgtc cagatgcggc agcagttttt
     8521 tgcacaaatc gaccagcttt tgcgcgtctt tgtcaccgga acgggcgcgt ttttcttcgc
     8581 ggacgatggc tttttccaca cttgccaagt cggcaagcgc caactctgta ccgatggttt
     8641 cgatgtcggc aatcggatcg actttgccgg aaacgtggac gatgttgtcg tcgtcaaagc
     8701 agcgcacgac attcacaatc gcatcggttt cgcggatgtt ggcaaggaac tggttgccca
     8761 agccctcgcc tttgctcgcg cctgcaacca aaccggcaat atcgacaaat tcgacgatgg
     8821 caggctgcat tttttgcgga ttgacgattt ttgccaattc ggccatacgc ggatcgggga
     8881 cttcgacgat gccgacgttg ggttcgatgg tacagaaagg atagtttgcc gcttcgatac
     8941 ccgattgggt cagcgcgtta aaaagggtgg atttgccgac gttgggcaaa ccgacgatgc
     9001 cgcatttcaa actcatgttt tttcctgaaa atagagaaat ttaacggcgg attatagcat
     9061 accgccgccc gcgttccgaa aaatgccgtc tgaaacggct tcagacagca tccggtttca
     9121 aaaaccgttc agaacaagcc gtgaatcacg ccgtgttcgt ccacatcgat tttctcggcg
     9181 gccggaactt tcggcagacc cggcatcttc atcatattgc cgcacagcgc gacgatgaag
     9241 cccgcgccgg cggaaacggt gatgccgcgc acggcgatgc ggaagccttc ggggcagccc
     9301 aacagtttgg cgttgtcact caaagagtat tgggttttcg ccatgcagat cggcattttg
     9361 tccaagccca gtttttccag tgaagcgatt tcggcagacg cttccgcgct gaaatcaaca
     9421 tcttccgcgc cgtacacttt ttgggcaatc gcacggattt tgtctttgat gcccaactcg
     9481 acatcgtagg cgaaaccgaa gttattggtt tgactttcaa tggcgttgac gactttgcgc
     9541 gccaaatccg cgccgcccgc accacctttg ccccacactt cggtcaggga aacttcaacg
     9601 ccgtgttcgg cacaggcttt ttcaatcatc gccaactcgg catcggagtc ggacacgaag
     9661 cggttgagcg caacgacgac gggcagtccg aatacgtttt tcaggttgga aatgtgtttc
     9721 agcaggttgg gcaaaccttt ttccaaagcg tctaaatttt cttcgccgag gttggcgcgt
     9781 tccacgccgc cgttatattt caacgcgcgg acagtcgcca cgacaacagc cgcatcaggt
     9841 ttcaaaccgg caaggcggca tttgatgtcg cagaattttt ccgcgcccaa gtccgcgccg
     9901 aagcctgctt cggttacggc gtaatcggca aggtgtttcg ccagacgggt tgcggttacg
     9961 gagttgcagc cgtgggcgat gttggcgaac gggccgccgt gtacgaaggc gggcgtgcct
    10021 tcgatggttt gcaccaagtt gggcttaatc gcatctttaa gcaatgccgc catcgcgcca
    10081 ttcgctttca aatctttggc gtaaacgggg ctgccgtctt tggcgtaggc gacaaggatg
    10141 ttgcccaaac gctctttcaa atcgctgatg tctttggcaa gacagaatac cgccatcact
    10201 tcggaagcaa cggtaatatc gaaaccgtca ggacgcatca cgccgtcaac aggcttgccc
    10261 atgccgtcga tgatgttgcg caactggcgg tcgttcatat cgaccacgcg ccgccacagc
    10321 acgcgtttgg ggtcgatgtt caactcgttg ccttggtaga tatggttgtc gagcatcgcg
    10381 gcaagcagat tatttgccgc accgatggcg tgaaaatctc cggtgaagtg caggttgatg
    10441 tcttccatcg gcaaaacttg ggcatagccg ccgcctgccg cgccgccttt cacgccgaac
    10501 accggcccta aagaaggttc gcgcagggca atcacggcat ctttgccgat gtggcgcaac
    10561 gcgtccgcca aaccgatggt tacggtggtt ttgccttcgc cggcaggagt cgggttgatg
    10621 gcggttacca aaatcagcct gccttgtttt tgcggcagtt taaacgcttc ggcaggattg
    10681 attttggctt tgtaatgacc gtaaggctca atgttgtcgg cgttcaggcc cagcttggcg
    10741 gcaatttcgc caatcgggcg catggtggaa gactgggcga tttcggcatc ggttttgaag
    10801 ctcatgattt tcctttagaa atgaggaggg acataccgtc tgaaagcatc aggcgacaaa
    10861 caggtggatt gaaaataata tcaggcatat tataacgtta tccgcaccaa acccgcagtg
    10921 aaatttttga cgctgcaaca aaaataccgt tcatattgtt cacaatccaa ggagaaaaca
    10981 tgggcagcaa cgcatggctg ttttgggcat tggcatcggc aggcttcgcc tcattgaccg
    11041 ccgttttcgc caaaatgggc ttgcagggta tagattcaga tttcgctacc ttcatccgca
    11101 ccttggtcat cctcgccgct ttggtactgt ttttgaccta caccggcaaa tggcagggcg
    11161 tgaacggctt tacggggcac aactggacat tccttatcct atccggcctt gctaccggcg
    11221 catcttggct tgcctacttc aaagccctgc aactgggtaa cgcctcgcaa gtcgcccccg
    11281 tcgataaatt cagcctggtt ctggtcgcgc tgatggcggt ggttttcttg gacgaacgcc
    11341 cgaacacgca ggaatggata ggcttggggc tggtaacggc gggcgtgttg gtgctggcgt
    11401 tgaaacgtta aaccgaatcc gccataccgc ctgaaaccgg gttttgacaa tctgacgggc
    11461 ggcggtctga acaaagttac ccaataaaat ccgacaccgc gccgacggca aaccgccgcg
    11521 cttcagatgg catttgttgg acggaaagcc ccgaacgccc tattttcacg cctccacgaa
    11581 aatatgccgg acatccggtc ggaaccgttc ggcagccggc tcggcaagcc accgttttat
    11641 ttcacttcca agcccctgca agggcttgag cctctttcag acggcatacc gtgccgacat
    11701 ccagccacaa gcccgtatgc ttctgaccgc tcacgcggtt ttgccgcatt tcgttccgca
    11761 atacgggcgc gagtttcgcc acactgcccg cttcgattcc gtcaaacatt tcaggacggt
    11821 aaatacccac gccgctaaat gtcaatccgt tgccgccatt tacttccggc cgcacgctgc
    11881 cgtcgggcag cagggaaaaa tcgccgtcag gatggtgcgg gggattttcc accagccaca
    11941 gatgggcgga aatgtgctcg ggcagggacg atgccgcctg aaacgcggcg gtaaaatcga
    12001 tgtcggtcaa cacgtcgccg ttgaccacca aaaacggggc atcgcccaac agcggcaatg
    12061 cccgtgcgat accgcctgcg gtttccaagc cgccttcagg ttcgggcgaa taggcgatgt
    12121 tcacgccata agccgagccg tcgcccaaag cctcttctat ctgccgaccc agccaagcgt
    12181 ggttgatgac gatttcggta aaccctgcct gcttcagacg gcataggtgc caaccgatta
    12241 gaggcttacc cgccacatcg agcagcggct tcggagtggt atcggtcaaa gggcgcatac
    12301 gctcgccgcg tcctgccgcc agtatcatcg ctttcatata tatctgtccg aatatcagtc
    12361 taaagatcta aactgccgtc tgaaataaag cagcgcggga cgtttgccca cgcaattttt
    12421 aatttcgtcg agcctgacgt aaaacacaaa atgtgtgccg atttcatgtt ggccgacaat
    12481 atgcccgtgc agatgcgcca acgcgccttc tatttcaggt tgtccggttt taccgtaatg
    12541 ccaaatatga taggcaaaac gttcttcggg tgaaagattg gtcaatccgg caaaatgttc
    12601 ggccacatcc tgatgttcgt ctgccaacgt attgatgcac aggctgccgt tttccgacag
    12661 gatcggaatg attcgcgcac tccggttgat gcacagcatc acggtcggcg gctcgtcggt
    12721 aaccggcgcg accgccgtca ttgtaatgcc gtaacgccct gccgcaccgt ctgtcgtgat
    12781 gacatgaacg cctgccgcgc aagatgccat cgcatcacgg aacgaagttt gaaaagtttt
    12841 ctgcaaatcc gccatttttc ccctttaaac tgtcccctat ataagaatgc tgcacacaag
    12901 gcacccccca tgtgcagcag ttctgattca aaaagccgtc ggtcggacat ttccgcgcgt
    12961 tacggcgtat tacgagttca acgcatcctc gattttggca agttctgcca acaggtcttt
    13021 aagcagcagc attttctcgc ggcccagcac ttcctcgata gcgtcgtaac gctcgtccac
    13081 ttcttcgccg atttcctcat acagcttctc gccctcggca gtcagcttca gaaaaacacg
    13141 tcgttggtcg ttggaaggtt tcaggcggac aaccaaaccc gctttttcaa ggcgggtcag
    13201 gataccggtc aggctggggc gcaaaatgca cgcctgattc gccaaatctt gaaagtccag
    13261 cgtgccgttt tccgccaaaa gacggataat ccgccattgc tgatcggtaa tattcgcctg
    13321 attcagaata ggcctgaatt gggtcatcag ggcttccctt gcctgtatca gactgatatt
    13381 gatagacgca tgttttgatt gggtaggcat tgttcaagtc tccaagttat cgaaaatcaa
    13441 actttcaaac cgtcgggaaa gcctgtgggc gtaatttttg atgcaaccgt tatataacaa
    13501 aacgaacata tagcaacaat acgctataaa ccgcatcgga cgactgggta taaaagactt
    13561 taattccgat aatcctatct aaaaatattt taatagttat atcttagtct attttcccta
    13621 caatctcaac aaagtattac atttactgtc gaaagaactt tcttccagaa aaaacaaaag
    13681 ccgccgcatc agacgagcaa ggtatatgcc gtttgattct ctacatagcg taaagtttaa
    13741 caaaaacaaa agttaaccga aaacatccgc ctgaaaaatt cgtgcgcgca agccccaata
    13801 actgctgatt cccgtcgtat agtgaaccat tttcccattt ttgaccaaaa cgacggcagg
    13861 cgttgcgaca atccgccaag accttgccaa acccccgtcc tcatcgttga ccgtcggaaa
    13921 gcccaagccc cgctttgcca tatacgccgc cacttcggac gcgctgccgg aacgtaccgc
    13981 cacgccgacg accggaacgc cgtccgccgc caaatcatcg attatcggcg actgataacg
    14041 gcacacgccg caccagcttc cccaaaaata caccaaaacc gccttatccc ggctaaactg
    14101 tcccaaagtc agccgctgcc ccgacagcag ggtcaaaggc cgccctgccg cgccggccgg
    14161 ctcttcgggc ttgcgtaccc aatccaaaaa cagcgacacc aataaaaaca ccaatgccgt
    14221 ctgaacggca aatttgatgc ccgaaagcag tttctttttc atacgccccc tcaaacggta
    14281 cgcccgcgca accggcaggc aaacaaaaaa gccaagtctt aaaacttggc ttccggttat
    14341 ctggtgggtc gtgagcgatt cgaacgctcg accaacggat taaaagtccg ctgctctacc
    14401 ggctgagcta acgacccgat aagccgtgca ttatacagca ccatcctgcc tcgtcaagca
    14461 aattttacag gcttaattgc agaccactgt ttgcacggga tattttgaca acggattttc
    14521 acaatccgcc gcataccgtg taaaagttcg cacaaggaaa ggcaaaccgc ccgaaatcaa
    14581 tgtacacttt ccgcccgttt cccaaactgc acacagaaac acacattatg aacatacaaa
    14641 acatccgcac cctcctcgac accgtcgccg ttccgaatac ggcacgcacg ctcggcggcg
    14701 aaaaggccgt ccgttcggtc gaacagcgtt cagacggcat ccatatcgcc ctgcatttcg
    14761 gcttccccgt cgcgcacatt gcctcagaaa cagccgaccg catacaggaa atcctgatgc
    14821 cgctgacagg cgacacacac atccatctgt ccatggacac ggaaatcggc acacacaaag
    14881 tccagcccgg cgttaccact atcaaaggtg tgaaaaacat catcgccgtc gcctccggca
    14941 aaggcggcgt gggcaaatcg acgacgaccg ccaaccttgc cgccgcaatg gcgcggatgg
    15001 gcgcgcgcgt cggcgtactc gatgccgacc tttacggccc gagccagccc acgatgctgg
    15061 gcgtacacga ccgcaaaccc gatcagaaaa accaaaaact cattcccgtc gaatcttcag
    15121 acggcataca ggtcatgtct atcggctttc tcgtcgatac cgaccaagcc gtcgtctggc
    15181 gcgggccgat ggtcagccaa gccttgcagc aactgatgtt tcaaagcgag tgggacgaag
    15241 tggattatct tttcatcgac ctgccccccg gcacgggcga catccaactc acactgtccc
    15301 aacgcatccc cgttaccggg tccgtcatcg taaccacgcc gcaggacatc gcgctgatag
    15361 acgcgcgcaa ggctgtggat atgttccgca aggtcaacat tcccattttg ggcgtattgg
    15421 aaaatatgtc cgtccacatc tgctccaact gcggacacag cgaagcactg ttcggcacag
    15481 acggcggcaa agatttcgct gcacgcctca acgtccccct gctcggacag cttcccttaa
    15541 gcctgcccgt gcgcgaagca atggacggag gggcggcggc acaactgttc gacgaacacc
    15601 ccgccatcgc ccgaatctac accgatgccg cattccaaat cgccctgagc attgccgaca
    15661 aaggcaaaga cttcagcagc cgcttcccca aaatcgtcgt cgaataaagc cgcgtccgaa
    15721 accgcaacag caatgccgtc ccaagccccg cgcctgccgg cgggcttcat agtcctctct
    15781 ccccgtggga gagggctgga gagagggcaa caagccgcaa ggcttgtatt ggggcggtta
    15841 agctgttggg aaagattgcc gcaacttgga gaatgccctc tccccggccc tcccccacag
    15901 gggagggagc ggattgcggc aggttttgcg gttgcaggcg gtttgaaaaa cagattggat
    15961 ttacaaccgt tgatgcaggt cgtctgaaaa gcaaaaagca gcctgcacaa cctgattagt
    16021 ttacaaaacc cttaacccca acagcctccc cgtcctctct ccccgtggga gagggctgga
    16081 gagagggcaa caagccgaaa ggcttgtatt ggggcggttg agttgttggg aaaggttgcc
    16141 gcaacttgga aaatgccgcc tccccggccc tcccccacgg gggagggagt gggtttcggc
    16201 agatttttcg gttgcaggcg atttgaaagg caacttagat ttgcagctgt tgtttcaggt
    16261 cgtctgaaaa ataaaaagca gcctgcacaa cctgttttcc ttgcagcaaa cctcaacccc
    16321 aacagccgac ccgtcctctc tccctgtggg agagagctag agagagggca acaagccgaa
    16381 aggcttgtat tggggcggtt gagttgttgg gaaagattgc cgcaacttgg aaaatgccgc
    16441 ctccccggcc cgccattgcc gcgcaggcgg caatggcggg cctgccggcg ggcaaacttg
    16501 ccggataaaa cggttttttt gagattttac gttccggatt cccgctttcg cggcaatggc
    16561 gggccaagga ggcggttgcg gattgccgaa aaccgcttta ccatgatcag cgcgcaaggg
    16621 tagaataatg cggtaaccct atacattgca tccggccaaa ggggcgtgtt acttttccga
    16681 acatccccct ttggcagccg ggccaaaggg gttttccaac cagcaattcg gcgggcgcgg
    16741 aatcgggcgg tttaccgaac cccggcgttc gcggcgcgcc tgccgccgac ggtatcccgc
    16801 gaagcaagat ttaagggata aaatatgttc caacacgcag ggcggcacat aaggcgccgc
    16861 cctgattcgg aagggcttgc acccctcccg gacaaagcct gatcctgccg ccccgaagga
    16921 cggatgcccg aagggcgggg ggtttgaccg aaaaggaaat acgatgaata aaactttaaa
    16981 aaggcgggtt ttccgccata ccgcgcttta tgccgccatc ttgatgtttt cccataccgg
    17041 cggggggggg gcgatggcgc aaacctataa atacgctatt atcatgaacg agggaaacca
    17101 gctcgaggta aaggggaatg ggcaatattc aacaataaag gacaaagaca gggaacgcga
    17161 atatacttat catcagggca gaggaggagg aggctctgtc tcattcaaca atagcgatga
    17221 gcttgtttct cgacaaagcg gtactgccgt ttttggcaca gccacctacc tgccgcccta
    17281 cggcaaggtt tccggttttg atgaaagaaa gctgaaagag cgcaccaatg cccttaattg
    17341 gattcatacg acccacccag ggttgatagg ctacagctac gccggtgtcg tatgcagaga
    17401 cagcacaggc tgtcccaaac ttgtctataa aacccaattt accttcggta attccggttt
    17461 ggcaaaaaag gcaaacggcg gcgggctgga tatatacgta gacaaaagcc gcgacaattc
    17521 gcccatttac aaattgaaag atcatccatg gttgggcgta tctttcaatt tgggcagcga
    17581 gaataccgtc aaaaatagca aatcatccaa caaattgata tcttctttta gtgaagacaa
    17641 taataatcaa accatcgtct ctacgacaga aggctcccct atttcccttg gcgaccagca
    17701 gcgcgaacat accgccgtgg tctattatct gaacgccaag ctgcacctgc tggataaaaa
    17761 acagattcaa aatatcaccg acaaaacagt gcagttgggt gtcttgaagc cgagcatcga
    17821 tgtgcggcag cagaaaggtg ccaactggct aagtttttgg gctagttggg acattaaaga
    17881 taccgggcag attccagtca agctcggcct gcagcaagtc aaagcaggcc gctgcatcaa
    17941 taaaccgaac cccaatccca acaaaaaaga cctttcgccg gccctgactg cccccgcgct
    18001 gtggttcgga cctgtgaaag atggtaaggc ggagatgtat tccgcttcgg tttctaccta
    18061 ccccgacagt tcgagcagcc gcatcttcct tcaaaatctg gaaagaaaaa ccgaccccgg
    18121 cagacccggc cgccattccc tcaaaccctt gagtgatacg cagattaaaa gtagagagcc
    18181 gaatttcacg gggcggcaaa cagtcatccg attggataaa ggcgtacatc agatcaaact
    18241 taaaggcaat gaggtcgaag gttttaaggg aaacaacggc aacgacactt tcggcattgt
    18301 tagagaaggg agcttcacgc ctgatgccag cgaatggaaa aaagtgctgc tgccttggac
    18361 ggttcgagat tccaatgatg acggtcgatt taacgcagtc aacaaagaag aaaacaacgg
    18421 caagccaaaa tacagccaaa gataccgcat ccgcgacaac aacggcaatc gcaatttggg
    18481 cgacatcgtc aacagcccca tcgtggcggt cggcgagtat ttggctactt ccgccaacga
    18541 cgggatggtg catatcttca aaaaaaacgg cggcggggat gaccgcaact atagtctgaa
    18601 gctcagctac atccccggca cgatgccgcg caaggatatt gaaaacaaag aatccaccct
    18661 tgccaaagag ctgcgcgcct ttgccgaaaa aagctatgtg ggcgaccgct acggcgtgga
    18721 cggcggcttt gtcttgcgcc gcattacaga tgaccaagac aagcaaaaac atttctttat
    18781 gtttggtgcg atgggcctgg gcggcagagg cgcatacgcc ttggatttaa gcaaaatcga
    18841 cagcaataac ccgaccgccg tttccctgtt tgatgtcaaa catgacaata gtggcaataa
    18901 tagcaataat agcaataata gcaataatag cgtgcaatta ggctacaccg tcggcacgcc
    18961 gcaaatcggc aaaacccaca acggcaaata cgccgccttc ctcgcctccg gttatgcgac
    19021 taaaaccatt gacagcacag acaataaaac cgcgctgtat gtgtatgatt tggaaagcaa
    19081 caacggtacg ccgattgcaa aaatcgaagt aaccggcggc aaaggcgggc tttcgtcccc
    19141 cacgctggtg gataaagatt tggacggcac aatcgatatc gcctatgccg gcgatcgcgg
    19201 cggcagtatg taccgctttg atttgagcag tcaagatcct aaacaatggt ctgcacgcgc
    19261 catttttaaa ggcgacaaac cgattacttc cgcgcctgct atttcccaac tgaaagacaa
    19321 acgcgtggtt atcttcggca cgggcagtga tttgagtgag gatgatgtac tcagtacgga
    19381 tgaacaacat atttacggta tttttgacaa tgacacaaac acgggtacgg cgcaagacgg
    19441 gcagggcaac gggctgctcg agcaagtgct gaaaaaagac ggcaatacgc tgttcttaag
    19501 cgactacaaa aggtctaacg gctcgggcga taagggctgg gtagtgaaat tggaagccgg
    19561 acagcgcgtt accgtcaaac cgaccgtggt attgcgtacc gcctttgtaa ccatccataa
    19621 atatacgggt aatgacaaat gcggcgcgga aaccgccatt ttgggcatca ataccgccga
    19681 cggcggcaag ctgaccaaga aaagcgcgcg cccgattgtg ccggcagcca attcgaaggt
    19741 cgcgcaatat tccggcgata agaaaacttc cagcggcaaa tccatcccta taggttgtat
    19801 ggaaaaagac aatggaatcg tctgcccgaa cggatatgtt tacgacaaac cggttaatgt
    19861 gcgttatctg gatgaaaaga aaacagacga tttccccgtc acggcagacg gtgatgcagg
    19921 cggcagcgga acattcaaag agggtaaaaa acccgcccgc aataaccggt gcttctccgg
    19981 aaaaggtgtg cgcaccctgc tgatgaacga tttggacagc ttggatatta ccggcccgat
    20041 gtgcggtatg aaacgaatca gctggcgtga agtcttcttc tgatttgcac gcgaaaatgc
    20101 cgtccgaaag gttttcggac ggcatttttt gcgtttttcg ggagggggca aatgaaacg
//
LOCUS       DAWHXS010000031        19779 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWHXS010000031 DAWHXS010000000
VERSION     DAWHXS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.031"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..538
                     /gene="pglH"
                     /locus_tag="WIE22_000845"
     CDS             <1..538
                     /gene="pglH"
                     /locus_tag="WIE22_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ4126958.1"
                     /translation="FRSKTTRLFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGG
                     GPDLEKLKQYENRAIKFYGYLPYSEMMSIAKGCDIAVNAIHSHAMQSVTNKLSDYMAL
                     QKPILNSQTNAEVLSLLNLLPHENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFR
                     RDISYRKIVNLIERLANE"
     gene            531..2108
                     /locus_tag="WIE22_000846"
     CDS             531..2108
                     /locus_tag="WIE22_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ4126959.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHYSFWLDMKILFLTVRKVFIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMAVLQGARGCI
                     TAQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            2095..2781
                     /locus_tag="WIE22_000847"
     CDS             2095..2781
                     /locus_tag="WIE22_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4126960.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            2829..3023
                     /locus_tag="WIE22_000848"
     CDS             2829..3023
                     /locus_tag="WIE22_000848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126961.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAAAAQKPRDRDGISD"
     gene            3147..4322
                     /locus_tag="WIE22_000849"
     CDS             3147..4322
                     /locus_tag="WIE22_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ4126962.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHNLWVVEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKELWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTVCRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            4370..6280
                     /gene="pglD"
                     /locus_tag="WIE22_000850"
     CDS             4370..6280
                     /gene="pglD"
                     /locus_tag="WIE22_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ4126963.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPAFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGKGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            6336..7628
                     /locus_tag="WIE22_000851"
     CDS             6336..7628
                     /locus_tag="WIE22_000851"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ4126964.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(7680..8516)
                     /locus_tag="WIE22_000852"
     CDS             complement(7680..8516)
                     /locus_tag="WIE22_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ4126965.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYAFLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            complement(8912..9115)
                     /locus_tag="WIE22_000853"
     CDS             complement(8912..9115)
                     /locus_tag="WIE22_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126966.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(9093..9326)
                     /locus_tag="WIE22_000854"
     CDS             complement(9093..9326)
                     /locus_tag="WIE22_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ4126967.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            9864..13298
                     /gene="dnaE"
                     /locus_tag="WIE22_000855"
     CDS             9864..13298
                     /gene="dnaE"
                     /locus_tag="WIE22_000855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ4126968.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPNAPDKPFRAMLIIRNDAGYQRLSELLT
                     EAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGMNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPDQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDLPRWSESEKLAEEKTVIGFYLSGHPFGPYAQEVCQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGSQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            13576..16824
                     /gene="cas9"
                     /locus_tag="WIE22_000856"
                     /pseudo
     CDS             13576..16824
                     /gene="cas9"
                     /locus_tag="WIE22_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes.;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
     gene            16890..17804
                     /gene="cas1"
                     /locus_tag="WIE22_000857"
     CDS             16890..17804
                     /gene="cas1"
                     /locus_tag="WIE22_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ4126969.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFIADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARTLTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKAELTPGIKQHLIKTLHYQISIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKEYRYE"
     gene            17797..18123
                     /gene="cas2"
                     /locus_tag="WIE22_000858"
     CDS             17797..18123
                     /gene="cas2"
                     /locus_tag="WIE22_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ4126970.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   18187..19278
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=18187..18222
                     /rpt_unit_seq="attgtagcactgcgaaatgagaatgggagctacaac"
     gene            complement(19565..>19779)
                     /locus_tag="WIE22_000859"
     CDS             complement(19565..>19779)
                     /locus_tag="WIE22_000859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4126971.1"
                     /translation="ETVDVFSNHGLGGLLKEGAHEKSPKCLGGNLGDFGEFCKGLILQ
                     DFSLIIKQGTAKIPAATGIFVRLSPV"
BASE COUNT         5225 a         5460 c         4820 g         4274 t
ORIGIN      
        1 attccgctcc aaaaccaccc gccttttcta cttgggaacg ctcagttaca gctacgacgt
       61 ggaaaccgtg tgcaaaggtg ttcggaaact tttagacgac ggcgaaaacg tcgaattgca
      121 cattatgggc ggaggacccg atttggagaa actcaaacaa tatgaaaatc gcgccatcaa
      181 gttttacggt tatttaccct attctgaaat gatgtcgatt gccaaaggct gcgacattgc
      241 cgtcaacgcc atccatagcc acgccatgca gtcggttacc aacaaactct ccgactatat
      301 ggctttgcaa aaaccgattc tgaacagcca gaccaatgcc gaagtcctta gcctcttgaa
      361 cctgctgccg cacgaaaact accgttccgg cgacgtggac agcttcgttc aagccgccaa
      421 agatattttg aaacgcaaaa acgaccccgt tcagtccgat gaaatcgtcc gccgcttcag
      481 gcgcgacatt tcctatcgga aaatcgtcaa cctgattgaa agattggcaa atgagtaaag
      541 ccgtcaaacg cctgttcgac atcatcgcat ccgcatcggg gctgattgtc ctctcacccg
      601 ttttcctgat tttggcgtac ctcatccgca agaatctggg ttcgcccgtc ttcttcattc
      661 aagaacgccc cggaaaggac ggaaaacctt ttaaaatggt caaattccgt tccatgcgcg
      721 acgcgcttga ttcagacggc attccgctgc ccgacagcga gcggctcaca cccttcggca
      781 aaaaactgcg tgccgctagt ttggacgaac tgcctgaatt atggaatgtc ttaaaaggcg
      841 acatgagcct ggtcggcccg cgcccgctgc tgatgcaata tctgccgctg tacgacaact
      901 tccaaaaccg ccgccacgaa atgaaacccg gcattaccgg ctgggcgcag gtcaacgggc
      961 gcaacgcgct ttcgtgggac gaaaaattcg cctgcgatgt ttggtatatc gaccattaca
     1021 gtttttggct ggatatgaaa atcctgtttt tgacagtcag aaaagtcttt atcaaagaag
     1081 ggatttcggc ggaaggggaa gccactatgc cttatttcac tggaaatcaa actaatgaaa
     1141 aaaataatat actgattctg tcggcaggtc gccgtgtcga attggtgcag gacttccaaa
     1201 ccgaagccgc acgtttttca gacggcatca gcgtttttgc cactgacctc aacccccgca
     1261 tgtcttctgc ctgccatgtt tcggacggtt catttgaagt gccgccgatt agtaccgatt
     1321 catatatcga cagcattttc aatctggcgg tacgcgaaaa tatcggttta atcatcccaa
     1381 ccatagacac cgaactgcaa aagctggcag acgagcgcga acgctttgaa gcggcaagtg
     1441 tccacatcgt cgtttccgat tcggacttca tcgcacaatg ccgcgacaaa cggaaaaccg
     1501 ccaccttgtt tgcccgctac ggcatacgct cacccgaaat ttacgaccgc aagaagttgg
     1561 ttttcccttg cttcgccaag ccttatgacg gcagccgcgc catcggtgca aaacggattg
     1621 acacacctgc cgacctgaca ccggaagtct tggcagacac caaattaatg ttctgtcagt
     1681 ttatcgacat cgaaaataaa ttttccgagt ttaccgtcga tatgtattac gaccgtcaat
     1741 gccgtctgaa atgcgccatc ccgcgaaaac gcttggaagt gcgctccggc gaaatcagca
     1801 aaggcgttac ctgcaaaaac agcctctacc aaacgctgct ggaaaaaatg gcggtattgc
     1861 agggcgcgcg cggctgtatc acggcgcagt tcttctacaa cgaagaaacc ggcgaattct
     1921 acggcgtgga aatcaacccc cgcttcggcg gtggcttccc gctgacgtat gccgcaggcg
     1981 gcaattatcc gagccggttg atgcgcgaat acctcggcga cgaagaaatc ccgttttcag
     2041 acgactggga aaacaacctc attatgttgc gctacgatgc caaggtcttg gttcatgaaa
     2101 acgattaatc ctgaaaccac cgtcatcgtc ttcgacttgg acgataccct gtattcggaa
     2161 tacgaataca agctctccgg tatccgttcg gtcatcgata ccgttgccgc actgtatccc
     2221 gattggaatt cagacaacct atggcgcaac atcgaccccg acggcaaaga ttggctggac
     2281 aaattgtgcc gccactgtgg ttttaatgaa tcggaaaaac aggttctttt gtggcaatac
     2341 cggctgcacc gcccgacgct gactccgtat gctccgccgg atttcctgtc cgaactgacc
     2401 gcgcccttcg ccgcacgcgc gctgattacc gacggaagaa gcctgaccca acgcctgaag
     2461 ctggaagcct tgggtctgtc ttccctgttt gacgacatcc tcatctctga agcctgctct
     2521 tcggaaaagc ccgacggcaa acgcttccgc catcttcaag acaaatatgc cgacaaggca
     2581 ggctgcttca tctatatcgg cgacaacatt tccaaagact ttatcgcgcc gaacactttg
     2641 ggctggataa ccataggatt gcttccctcc ggacagaaca tccaccgcca ccagcctgaa
     2701 agttttaaca cagaatatca tcccgacttt tgggttaact cccttcaaga tttagttcct
     2761 ataattgatt caaatactta accattacat aatcaaacat gcactcacaa tattaaaagg
     2821 actatttcat gacaaaatat tattcatggg gcgtaaacgg ctttccgccc tacgttttcg
     2881 acaccatttg gaaacgatgg aagttgaatg ggcgcggggg cggttatcgt gtgcgacatc
     2941 ccggacggca tgaccgtcgc gggcaatccg gcaaagccgc tgccgcgcaa aaaccccgag
     3001 accgcgacgg cataagcgat taaaatacac ccccgtacag accgattttg gcaacacctg
     3061 cggcgcgcgc ccgattcttc ggaacacgcc cccttcagac ggcatagagt cagaaatgcc
     3121 gtctgaaaac cgacggacca aacaccatgc tgaacacctc cctttccccg tggccctgct
     3181 tcacccaaga agaagccgat gccgtttcca aagtcctgct gtccaacaaa gtcaactact
     3241 ggacgggaaa cgaatgccgc aaatttgaaa aagaattcgc cgcctttgcc ggcacgcggt
     3301 acgccgtcgc cctttccaac ggcacgctgg cactcgatgt cgcgctcaaa gcaatgggca
     3361 taggcgcggg cgacgatgtg attgttacct cgcgcacttt cctggcttcc gcatcctgca
     3421 ttgtgaacgc gggcgcaaat ccggtgtttg ccgatgtgga tttgaacagc caaaacatca
     3481 gcgcggaaac cgtcaaagcc gtgctgaccc cgaataccaa ggcgattatc gtggttcacc
     3541 ttgccggtat gcctgccgaa atggatggca ttatggcttt ggcaaaagaa cataatcttt
     3601 gggttgtcga agactgcgcc caagcgcacg gcgcgaaata caaaggcaaa tccgtcggca
     3661 gcatcggaca cgtcggcgcg tggtcgttct gccaagacaa aatcatgacc acgggcggcg
     3721 aaggcggcat ggttaccacc aacgacaaag aactgtggag caaaatgtgg tcgtacaaag
     3781 accacggcaa aagctatgac gccgtgtacc accgcgaaca cgcccctggc ttccgctggc
     3841 tgcacgaaag cttcggcaca aactggcgta tgatggaaat gcaggcggca atcgggcgca
     3901 ttcagctcaa acgcctgccc gaatggactg tctgccgtca agcgcacgcc gccaagctgg
     3961 cggaaagcct aggcaaattc aaaagtatcc gcttggttga agtcgccgac tacatcggac
     4021 acgcgcaata taagttctac gccttcgtca aacccgaaca cctcaaagac ggctggacgc
     4081 gcgaccgcat cgtcaacgaa ctgaacgcgc gcaaagtccc ctgctatcaa ggtagctgct
     4141 ccgaagtgta tttggagaaa tccttcgaca acacgccttg gcgtccgaaa gagcggctga
     4201 aaaatgctgt cgagttgggc gacaccagcc tgatgttctt agtacatccg acgctgaccg
     4261 acggcgaaat cgcgttctgc aaagaacaca tcgaagccgt attgaccgaa gccacacgat
     4321 aacccttcag acggcatatg ccgcctgaaa gcacataccg ccccacgata tgactctgga
     4381 aaccctgctt gccctaccgc gcaacatcaa gaaaatctgt ttcctcatac acgattttct
     4441 gatgattttc attgcctttt ggtttaccca aagcctgaag gccgactact cgaacgaatg
     4501 gttcgaccct gccaactggc agtctttcct attgaccgcg ccgttgacca tcggcctgtt
     4561 cgtcagaatg gggctctacc gtgccgttac acgcttcatc agcttccgca tcctctccac
     4621 cgcgctggca ggcagcctcg cctccgccgt attgttcttc ctcaacacat tgatttttga
     4681 agaaaggctg cgcctcgccc tgccgattgt ctatttctta ctgctgtttg tttccgtgac
     4741 cggctcgcgt atggttttgc gcggactgtt gtccgaacac cccaaaaaac agatgatccc
     4801 cgtcatcatt tacggcgcgg ggcaggcggg cagacagctt ctcgaggccg tcaaacaaat
     4861 gcgcgaatat tccgccgccg cctttgtgga cgacgacccc aaactgtggc acaccgtcat
     4921 ctacgacctt gccgtttacc agcccgatgc catcgccttc ctcatcgaac gctacggcgt
     4981 ggaaaaaatc ctactcgcca tccccggcgc gacccaggaa caacgccgcc gaatcatcaa
     5041 caaactggaa gcctatccgt gcgaagtgtt gaccattccc ggaatgaaag acctgatgga
     5101 cgggaaaatc agcatcggca cgctcaaaaa aatctctgtg tccgacctgc tcgggcgtga
     5161 ttccgtcgcg cccgacaacc gcctgatgag tgccgacatc gaaggcaaaa ccgtcatggt
     5221 aaccggcgcg ggcggctcca tcggttcgga actctgccgc cagattatcc gccgccgccc
     5281 cgaaaagctg ctgctgttcg agttatccga attcgccctg tacgccgtcg aaaaagaatt
     5341 gcgcgaaacc tgcatccaaa aacgcctcga caccgaaatc ctgccctttc tcggttcggt
     5401 gcaaaaccgc acgctgctcg aacacgtcat gaccgccttt tccgttgcga ccgtctatca
     5461 cgccgccgcc tacaaacacg tccccatggt cgagttcaac accgtcgaag gcatacgcaa
     5521 caacatcttc ggcacactcg agtgcgcgct tgccgccacg acatcgggcg tggaaacttt
     5581 cgtcctcatc tccaccgaca aagccgtccg ccccaccaac accatgggtg ccagcaaacg
     5641 catggcggaa ctctgccttc aggcgctcgc cgccgaaccc ggacaaaaaa cccgcttcag
     5701 catggtgcgt ttcggcaatg ttttaggttc gtccggctcc gtcgtccccg catttgaaaa
     5761 acaaattgcc gaaggcggcc cgattaccct gacccaccaa gacattaccc gctacttcat
     5821 gaccattccc gaagcggcgc aactggttat ccaagccggc gcgatgggca aaggcggcga
     5881 cgtattcgtc ctcgatatgg gcgaatccgt caaaatcatc gaccttgccc gccaaatgat
     5941 taccttaagc ggcctcaaac ccaaaacacc cgaacaaccc gacggcgaca tcgaaatcct
     6001 cattaccgga ctgcgtcccg gcgaaaaact ctacgaagag ctgctcatcg gcgacaacgt
     6061 ccgcaaaacc ggccatccgc gcattatgac ggctgacgag gcgatgctgc cgtggcacga
     6121 gctctctgtc ctgctcgacc gcatccgtgc ggcctgcgac cgttacgacc agcaggcaat
     6181 ccgcaccctg ctcatcaacg ccccgaccgg ctttacccca agcgacggta tctgcgacct
     6241 gctttgggta cgagaaacac acagaaaaaa tgccgtctga accttcagac ggcataacgt
     6301 acaaaccaac ctaccttaca cacacggagt ttgacatgca gttctcagca ttcggcgaaa
     6361 aattcacgca acacagcggc atcctccaac tgatggacga cctcggcgac gcgctcaaaa
     6421 gcgacaagcc cgtcaatatg ctcggcggcg gcaatccggc gcgaatcgcc gaagtcaatg
     6481 ccgtgttcgc cgacgtattt tccaaactgg cagcagaaca tgccgtcgaa aacatcggca
     6541 actactccaa tcctcaagga gatgccgcgc tgattgacgc gctgaccgcc ttcctcaacc
     6601 gcgaatacgg ctggaacctg accgccgaca atatcgcgct gaccaacggt tcgcaaaacg
     6661 cgtttttcta tttattcaac ctcttcggcg gcaaattcaa cctttcagac ggcacatccg
     6721 cagaaaaagc cattttgttg ccgctcgcgc ccgaatacat cggctatgcc gacgtgcatg
     6781 tcgaagggca gcacttcgtt tccgtcaagc ccaaaatcga aaacgtcgaa cacgaaggcg
     6841 aagcaggctt cttcaaatac cgcgtggact ttgacgcact ggaaaacctg cccgaactca
     6901 aagcgggcaa aatcggcgcg atttgctgtt cgcgcccgac caacccgacc ggcaacgtgc
     6961 tgaccgacag cgaaatggcg cggctggacg ctttggcgca agaacacggc attccgctga
     7021 ttatcgacaa cgcctacgga atgccgttcc ccaacatcat ttacagcgac gtaacgctga
     7081 attggcatga aaacatcatc ctctgcttca gcctttccaa agtcggtctg ccgggcgtgc
     7141 gcaccggcat catcgtcgcc gcgcccgaag tcgtcaaagc cgtcagcagc ctgaacgcga
     7201 ttgtgaacct tgcccccacg cgcttcggcg cggccatcgc cgccccgcta ctggaaagcg
     7261 gcgagatgaa acggcttgcc gaccaagtca tccggccgtt ttaccgcaat caggcgcaaa
     7321 ccgccgtctc gctgctcaag cgcgagctgg gcgcgtaccc gatgaaaatc cacaaacccg
     7381 aaggcgcgat tttcctgtgg ctctggtttg aaaacctgcc cgtttcttcg caaaccctgt
     7441 acgaaatgct caaagccgaa ggtacgctga ttattccggg cgaacatttc ttcgtcggca
     7501 tcgacacgcg ggattatccg cacgcgggcg agtgcatccg tatgagcatc gcgcaggacg
     7561 ctcaaacgct ggaaaaaggc attgccgcca tcggtaaaac cgtccgaaaa ctgtacgaca
     7621 acgtttaaac cgcaaaaaat gccgtctgaa ggcctttcag acggcatttt tatccgcatt
     7681 caatgtcggg aaaaatgcgc ccacactggc ctgcagtttt ccggcagttc gggacacgcg
     7741 ccgaggatgc cgccgctgaa gtcgtttaaa cggtggaagt cgctgcccgc actggcgagc
     7801 atgccgaagc gttctgccaa aaatgcgtag ttgaggcggt cgtttttgca gcagttgccg
     7861 ctgtggactt cgatgcccgc gccgccgagg tttttaaatt cttcaaacag attgcgcttg
     7921 gcggtagcgg acaaatcata gcgcatcgga tgggcgatga ctgccatgcc gcccgcgccg
     7981 ttgacggcgg agacgcagtc ttccagcgtc gcccattcgt gtcggacggc gcaggatttg
     8041 ccgtcgccca agtatttggt gaacgcctgc tgcttgtttt tgacgtgtcc cacccgaatg
     8101 aggaactcgg cgacgtgtgt gcggctgacc atttctttgt ttgccgccag cgcgagcgcg
     8161 ccgtcgtatg cgcccgaaat gccgcgtttt tccagcttgg cggcgatggc ttcgaggcgt
     8221 ttcagacggc cttgccgcac ttgcgccaac aggttttgca ggttttcgtc ttgttcgtca
     8281 aagtctaagc cgacaacgtg tatggtacgc ccgcgccacg ttacggagat ttccacgccg
     8341 ttaatcaggc gcaaaccgag cctgtcggct tcggcacgcg cttcggcgat gccgccggtg
     8401 tggtcgtggt cggtcaacgc cagcagcgtg cagccgtttt gatgcgcgag gcgcacaact
     8461 tcggcggggg agagcatacc gtcggaaacg gtggaatggc agtgcaggtc tatcataagg
     8521 cgttatgtgc gtggtaaatg gtgggcaggg tgtttcaata ttgtcgggcg gtgtggcggc
     8581 ggtgatttca aaaaacaggt gtatggcaaa tgcaaaggaa aagccgctat gccgtcattc
     8641 cagcgcaggc gggaatccag actttgattt attggaaatg tttaaggtta accgctattt
     8701 caaacttccg gattcctgct ttcgagggaa tggcgatatg accgttttca attttaatct
     8761 actataaaag actgtctgaa aacgtggttt tatagtgaat taaatttaaa ccggtacagc
     8821 gttggctcgc cttgccgtac tatttgtact gtctgcggct cgccgccttg tcctgatttt
     8881 tgttaattca ctatatcaag ccgaaccgtt ttcagacggc atcgtccgac caacccgctt
     8941 ctttcaattt ccgccgttgc gcgtcgtagc gggccttatc cgcccaataa atcgtctgaa
     9001 tacacgcctc gccgcagtcg ctgccgcagc attcccacga ctcgggtcgg acgggttcgt
     9061 ctaaaagcgg ctcgcccaaa agggcttcgg ctttatgctt cagggtcata tccatcggcg
     9121 atttccaagc gcgcaccgtc aaactcgatg acttcgccgc cgcgtatttt ggcggtttta
     9181 cgggtttcgg tttcgccgtt gcgcgacacc aacccttcgg caatcaacgc cttcgcctgt
     9241 ccgccgcttt cggcaagtcc ggccaatttc aagaggtcgc acaaggcgat gtattcgttg
     9301 tcttcgagat agacgatggc ttccatgatg tccccttgca gaaagagacc gttattgtag
     9361 cacctgccgc cgccgtaccc aaaattaccg caaaaccagc gatgtatccg caccgcctgt
     9421 tccgtaaaag taaaaatgcc gtctgaaacc ccgtatgccg caatccgttc aaagaaatcc
     9481 tgcccaacgg cagactgcaa atcctgttcc ccgacgaatc cgcattgacg ctgatgcaca
     9541 tcctcaaaca cgaactgccc gatacaccgg caatcggcat caaaacgaaa tcaaaaacct
     9601 gtttgaacgt aaaattaatt taactgcttg ccatccgtcc gaataaggta tatagttcct
     9661 tataactagt ttgatagtcc tttatatcta tcaatactcc ttgggaagcc tccaccacac
     9721 ggtaggaggc atttttttgc cgtcgtaaaa gctcaaaaac atttgcaggt catgccgtct
     9781 gaatctgaaa cggcatgacc tacaccgcca tctaaagaca accctgctac aatacgcctt
     9841 ttattgttca cgccgatttt gccatgaccg agccgaccta catccccctg cgcctgcata
     9901 ccgagttctc aattaccgac ggtatggtgc ggattaaaaa actgattgcc aaagcgcagg
     9961 aatacggttt gcctgctttg ggcatcagcg atttgatgaa cgaattcggt ttggtgaaat
    10021 tctataaagc ctgccgcagt gcggggatta agcctgtcgg ggcggcggac gtgtggatag
    10081 gcaatccgaa tgcgcccgac aagccgttcc gcgccatgtt gattatccgc aacgatgcgg
    10141 gctatcagcg tttgagtgag cttttgacag aggcctatgt cggtaaagac cgcaatgtcc
    10201 atcatgcgga actcaacccc gaatggctgg aaaacggcga caacagcggc ttgatttgtt
    10261 tgagcggcgc gcattacggc gaagtgggca tgaatctgtt gaacggcaat gaagacgcgg
    10321 cgcgcgcggc ggcgttgaaa tatgcggcat ggtttcccga tgcgttttat ttagagctgc
    10381 aacgcctgcc cgaacgcccc gaatgggagg cttgcgtttc cggcagcgtg aagctggcgg
    10441 aggaactggg tttgccggtg gtggcgacgc atccgacgca gtttatgaac cgcgacgatt
    10501 tcaacgcgca cgaggcgcgg gtgtgtatcg cgggcggctg ggtattgacg gacaaaaaac
    10561 gtccgcgcga tttcacgccg gaccagtttt tcattccgcc ggaaacgatg ctggagcgtt
    10621 tcgccgactt gcccgaagcc ttggaaaaca cggtggaaat cgccaaacgc tgcaacctgc
    10681 acatcacatt gggcaaaaac ttcctgcccc ttttccccac gcccgacggt ttatcgcgcg
    10741 atgactatct catcaaactc tcaaacaagg gcttgcagga acgcatggtt cagctttatc
    10801 ccgacgaggc ggagcgtgcg gcaaaaatgc cggaatatca ggaacgtttg gattttgagc
    10861 tgaacatcat catccagatg aaattccccg gctatttcct tatcgtacaa gactttatca
    10921 actgggcgaa aacgcacggc tgtccggtcg ggccgggacg cggttcgggc gcgggttcgc
    10981 tggtggcgta ttcgctgaaa attaccgacc tcgacccgct caaatacgcg ctgctgttcg
    11041 aacgtttctt gaaccctgaa cgcgtttcca tgcccgactt cgacgtggac ttctgtcagg
    11101 caaaccgcgg ccgcgtgatt gaatatgtac gcgaaaaata cggtgcggaa gcagtcagcc
    11161 agattgttac cttcggcacg atgtcgtcca aagcagtgat tcgcgacgtg gggcgcgtac
    11221 tggaactgcc gtttatgctg tgcgacaaac tgtccaagct gattccgttg gaagccaaca
    11281 aacctttggg tttggacgac gcgatgaagg cggagccgca gattcaggaa ttaatcgaag
    11341 cggaagaagc ggacgaactg attacgctgg cgaaaaagct ggaagatttg acgcgcggtt
    11401 tgggtatgca cgcgggcggc gtgttgattg cgccgggcaa gatttccgat tacagccccg
    11461 tgtatcaggc ggacgaatcc gcctcgcccg tatccatgta cgacaagggc gacgtggaag
    11521 acgtgggttt ggtgaaattc gactttttag gtctgcgcaa cctgaccatc atcgaaatgg
    11581 cgcagaacaa catcaaaaac accaccggcg acatcatcga tgtcggcaaa attccgcttg
    11641 acgaccaggt tgcctaccaa atcttccgcg atgcgaacac caccgccgtg ttccagttcg
    11701 agtcgacagg catgaagaaa atgctgaaaa cagcgcatac gaccaagttt gaagaactta
    11761 tcgccttcgt atcgctctac cgacccggcc cgatggacaa tattcccgac ttcgtcgcgc
    11821 gtatgaaagg tcaggaattc caatacatcc acccgctact cgaaggcatc ctcgcaccaa
    11881 cctacgggat tatggtgtat caggaacaag tgatgcaggc ggcgcagatt atcggcggct
    11941 actcgctcgg cggcgcggac ctgctgcgcc gcgcgatggg taagaaaaag cccgaagaaa
    12001 tggtgaaaca ccgcgaaatc ttcgccgaag gcgcggcaaa acaaggcatt tcgcgcgaaa
    12061 aatccgacga aatcttcaac tatatggaaa aattcgccgg ctacggtttc aacaaatccc
    12121 acgccgccgc ctacgccctg atttcctacc agaccgcatg gctcaaggcg cactaccccg
    12181 ccgaatttat ggcggcgacc atgtcgtccg aattggacaa caccgaccag ctcaagcatt
    12241 tctacgacga ctgccgcgcc aacggcatcg agttcctgcc gcccgacatc aacgaatccg
    12301 actaccgctt tacgccgtat ccggacatga aaatccgcta cgcgctcggc gcgattaaag
    12361 gcacgggcga agccgccgtc gaatccatca ccgccgcgcg gcaaagcggc ggcaagttta
    12421 ccggcctgtt ggacttctgc gagcgcgtcg gcaaagaaca catgaaccgc cgcaccctcg
    12481 aagccctgat acgcggcggc gcgttcgaca gtatcgaacc caaccgcgcc atgctcttgg
    12541 cgaacatcga cctcgctatg gacaacgccg accaaaaagc cgccaacgcc aatcagggcg
    12601 ggctgttcga catgatggaa gacgccatcg aaccggtgcg gctcatcgac ctgccgaggt
    12661 ggagcgaatc ggaaaaactc gccgaagaaa aaaccgtcat cggcttttac ctgtccggcc
    12721 acccgttcgg cccgtatgcc caagaagtct gccaaatcgc accgaccaaa ttagaccgtc
    12781 tgaagccgca agacagcgtg cgcctcgccg gattcgttac cgccgtgcgt acgatgatgg
    12841 gcaagcgcgg caaaatcgcc ttcgtcagcc ttgaagattt gagcgggcag gttgaaatca
    12901 tggtcggcag tcagacgctg gaaaactgcg ccgactgcct caaagccgac caagtgctga
    12961 ttatcgaatc caaagtcagc cgcgacgact acggcggcgg cgacggactg cgcatcatgg
    13021 caaaccaagt catgaccctg caaacggcac gcgaacgcta cgcccgcagc ctcagcctcg
    13081 ccctcgcccc gcatcacgac atcggcggac tggtacggct gctctcctcc caccaactgc
    13141 ccgacacgcc gcgcatcccg ctgcaactgt cgtatgccaa cgaaaaagcg tcgggcaggc
    13201 ttcaagtgcc gccgaaatgg acggttacac cgagctccgc attgttcggc gaactggaaa
    13261 cattgctcgg cagccggtcg gtgcgcgtca actggtaacc caaaatataa atgccgtctg
    13321 aagcccaaaa tcggtttcag acggcatttt ttaaccgaaa taaacgatgc cccttaaagc
    13381 agaagcttta aggggcagag cgttgcggca catcttttca gacggcctta ttgtagcaac
    13441 ggttctcatt tcgcagtgcg acaatatgtt tgggattcta gccgttgtga gatgataagt
    13501 caataatcta tttgtatata aataagacaa taagatatat tatcattatt tgcaacgttg
    13561 tttacggagt aataaatggc tgccttcaaa cctaatccaa tcaactacat cctcggcctc
    13621 gatatcggca tcgcatccgt cggctgggcg atggtagaaa ttgacgaaga agaaaacccc
    13681 atccgcctga ttgatttggg cgtgcgcgta tttgagcgtg ccgaagtacc gaaaacaggc
    13741 gactcccttg ccatggcaag gcgtttggcg cgcagtgtcc gccgcctgac ccgccgtcgc
    13801 gcccatcgcc tgcttcgggc ccgccgccta ttgaaacgcg aaggcgtatt acaagccgct
    13861 gattttgacg aaaacggctt gatcaaatcc ttaccgaata caccatggca acttcgcgca
    13921 gccgcattag accgcaaact gacgccttta gagtggtcgg cagtcttgtt gcatttaatc
    13981 aaacaccgcg gttatttgtc gcaaagaaaa aacgagggcg aaactgccga taaagagctt
    14041 ggcgctttgc ttaaaggcgt ggccaacaat gcccatgcct tacagacagg cgatttccgc
    14101 acaccggccg aattggcttt aaataaattt gagaaagaaa gcggccatat ccgcaatcag
    14161 cgcggcgatt attcgcatac gttcagccgc aaagatttac aggcggagct gattttgctg
    14221 tttgaaaaac aaaaagaatt tggcaatccg catgtttcag gcggccttaa agaaggtatt
    14281 gaaaccctac tgatgacgca acgccctgcc ctgtccggcg atgccgttca aaaaatgttg
    14341 gggcattgca ccttcgaacc ggcagagccg aaagccgcta aaaacaccta cacagccgaa
    14401 cgtttcatct ggctgaccaa gctgaacaac ctgcgtattt tagagcaagg cagcgagcgg
    14461 ccattgaccg ataccgaacg cgccacgctt atggacgagc catacagaaa atccaaactg
    14521 acttacgcac aagcccgtaa gctgctgggt ttagaagata ccgccttttt caaaggcttg
    14581 cgctatggta aagacaatgc cgaagcctca acattgatgg aaatgaaggc ctaccatgcc
    14641 atcagccgtg cactggaaaa agaaggattg aaagataaaa aatccccatt aaacctttct
    14701 tccgaattac aagatgaaat cggcacggca ttctccctgt tcaaaaccga tgaagacatt
    14761 acaggccgtc tgaaagaccg tgttcagccc gaaatcttag aagcgctgtt gaaacacatc
    14821 agcttcgata agttcgtcca aatttccttg aaagcattgc gccgaattgt gcctctaatg
    14881 gagcaaggca aacgttacga tgaagcctgc gccgaaatct acggagacca ttacggcaag
    14941 aaaaatacgg aagaaaaaat ttatctgccg cccatccctg ccgacgagat ccgcaacccc
    15001 gtcgtcttgc gcgccttatc tcaagcacgt aaggtcatta acggcgtggt acgccgttac
    15061 ggctccccag ctcgtatcca tattgaaacg gcaagggaag taggtaaatc gtttaaagac
    15121 cgcaaagaaa tcgaaaaacg ccaagaagaa aaccgcaaag accgggaaaa agccgccgcc
    15181 aaattccgag agtatttccc caattttgtc ggcgaaccca aatcaaaaga tattctgaaa
    15241 ctgcgcctgt acgagcaaca acacggcaaa tgcctgtatt cgggcaaaga aatcaactta
    15301 gtccgtctga acgaaaaagg ctatgtcgaa atcgaccatg ccctgccgtt ttcgcgcaca
    15361 tgggacgaca gtttcaacaa taaagtgctg gtattgggca gcgaaaacca aaacaaaggc
    15421 aatcaaaccc cttacgaata cttcaacggc aaagacaaca gccgcgaatg gcaggaattt
    15481 aaagcgcgtg tcgaaaccag ccgtttcccg cgcagtaaaa aacaacggat tctgctgcaa
    15541 aaattcgatg aagacggctt taaagaatga aatctgaacg acacgcgcta cgtcaaccgt
    15601 ttcctgtgcc aatttgttgc cgaccatata ttgctgacag gtaaagggaa aagacgtgtc
    15661 tttgcctcaa acggacaaat taccaatctg ttgcgcggct tttggggatt gcgcaaagtg
    15721 cgtgcggaaa acgaccgcca tcacgccttg gacgctgtag tcgttgcctg ctcgaccgtt
    15781 gccatgcagc agaaaattac ccgttttgta cgctataaag agatgaacgc gtttgacggt
    15841 aaaaccatag acaaagaaac aggaaaagtg ctgcatcaaa aaacacactt cccacaacct
    15901 tgggaatttt tcgcacaaga agtcatgatt cgcgtcttcg gcaaaccgga cggcaaaccc
    15961 gaattcgaag aagccgatac cccagaaaaa ctgcgcacgt tgcttgccga aaaattatca
    16021 tctcgccccg aagccgtaca cgaatacgtt acgccactgt ttgttttacg cgcgcccaat
    16081 cggaagatga gcggtgcaca taaagatact ttgagatctg ctaaacgatt tgttaaacat
    16141 aatgaaaaaa ttagtgttaa acgagtatgg ttaaccgaaa tcaagttggc cgaccttgaa
    16201 aatatggtta attataaaaa tggtagagag attgaattat atgaggctct taaggcgcgt
    16261 ttagaggcat atggaggtaa tgctaaacaa gcatttgacc ctaaggacaa tccgttttat
    16321 aaaaagggag gacaactggt taaagctgtg agggtcgaaa aaacccaaga gagcggagtc
    16381 ttattaaata aaaaaaatgc ttataccatt gcagataatg gagacatggt acgtgttgat
    16441 gtgttctgta aagtagataa gaaaggaaaa aatcagtatt ttattgttcc catctatgct
    16501 tggcaggttg ctgaaaacat tcttcccgat attgattgta agggataccg gattgatgat
    16561 agctatacat tctgttttag cttgcataag tatgatctga ttgcttttca aaaagatgaa
    16621 aaatctaaag tagaattcgc ctactatatc aactgtgata gctctaatgg acgattctat
    16681 ttagcttggc atgataaagg ctctaaagaa cagcaattcc gtattagcac ccaaaatctt
    16741 gtattgatac aaaaatacca agttaacgaa ctgggcaaag aaatcagacc atgccgtctg
    16801 aaaaaacgcc cacctgtccg ttaaattaat ctgtccctgc ttcagacggc ctgaagcagg
    16861 gattttttat atcaaaataa ggggaaaaca tgacttggcg cagcctactt attcaaaacg
    16921 gcggcaaact atccttgcaa cgccggcaac tgctgattca acaaaacggc gagtcccata
    16981 ccgtaccatt agaagacatc gccgttatca tcatcgaaaa tcgcgaaacc ctgattaccg
    17041 ccccgctcct atcagcctta gctgaacatg gcgcaaccct gctgacctgc gacgagcagt
    17101 tcctgccctg cggacaatgg ctgccttatg cccaatatca ccgccagctt aaaatcctca
    17161 agctgcaact gaacataagc gaacccttaa aaaagcaatt ctggcaacat atcgtccgcc
    17221 aaaaaatcct gaaccaagca tttatcgcag acgaaacagg caatgatctt gcagccaaac
    17281 ggctgcgcac tttggcatct gaagtccgtt cgggcgatac aggaaaccgt gaagcccaag
    17341 ctgccgccct ctacttccaa gccctctttg gcgaaaaatt tacccgtaat gacaacaatg
    17401 ccgtcaatgc cgcattgaac tacacctacg ccgtgctacg ggccgcagtt gcacgcaccc
    17461 tgaccttata cggctggctg cctgcattag gcttgtttca tagaagcgaa ctcaatccat
    17521 tcaatctcgc cgatgacttt atcgaaccct tacgcccatt agccgacctg accgtgatac
    17581 atttgtacga acaaggccgt ctgaaagccg aactgacccc cggcatcaaa caacacctta
    17641 tcaaaaccct tcactatcaa atcagcatcg aacggcaaca tttcagcacc ttggctgcca
    17701 tcgacaaaat ggtttcgtca tttcaagccg gtgtaaccga taaaaacgcc aaacaactga
    17761 aactgcctga aatcctacca ttgaaagaat accgatatga gtgaggccaa atttatgagg
    17821 attatcgtct tcttcgacct accggttatc acggcggcaa agcgcaaagc cgccaatcaa
    17881 ttccgccagt ttttattaaa agacggatac caaatgctcc agctttccgt atacagccgt
    17941 atcgtcaaag gccgcgattc gttgcaaaaa caccacaata gactatgtgc aaaccttccg
    18001 caagaaggct caatccgctg tttggagata acagaaaagc aatatgccgc catgaaactg
    18061 ctgttgggcg agctgaaaac ccaagaaaaa aaggtcaatt cagaccaatt attgttattt
    18121 taagcccatt ttttcagact aaataaaacg ggaaatcctt atagaataag gatttcccgt
    18181 tggggtattg tagcactgcg aaatgagaat gggagctaca acaagaagaa ggcggcgatg
    18241 ttttcgcctt taattgtagc actgcgaaat gagaatggga gctacaaccc ctgattgcag
    18301 gtgaagatga actgtatgat tgtagcactg cgaaatgaga atgggagcta caacaaaatc
    18361 gtcggcctgc tccagcacga cctgattgta gcactgcgaa atgagaatgg gagctacaac
    18421 ctacccgctc ggcgtatatg aggcaatcta attgtagcac tgcgaaatga gaatgggagc
    18481 tacaacgcgc acgattgacg gcagcagacg agccaaattg tagcactgcg aaatgagaat
    18541 gggagctaca acagcggtgg tagaatcgga tttttagagt gaattgtagc actgcgaaat
    18601 gagaatggga gctacaaccg tttttcaggt cgaattatcg aaccttcaat tgtagcactg
    18661 cgaaatgaga atgggagcta caaccaggtg cttgggcgta attttatccg ttggattgta
    18721 gcactgcgaa atgagaatgg gagctacaac ccttttgcaa caatcgtgct ttatttgtca
    18781 attgtagcac tgcgaaatga gaatgggagc tacaacttaa aatttacaac accgcccgac
    18841 ggcatgattg tagcactgcg aaatgagaat gggagctaca acgtcggcat tgttcagggt
    18901 gttcgcttcg caattgtagc actgcgaaat gagaatggga gctacaactc gcgcaagcat
    18961 tgacgatatt tgtgaggcat tgtagcactg cgaaatgaga atgggagcta caacaaattt
    19021 tatgaaaacc gtttccaccg ccgtattgta gcactgcgaa atgagaatgg gagctacaac
    19081 tatgcttggg tttgagtggg cgattgatga attgtagcac tgcgaaatga gaatgggagc
    19141 tacaacaaga agattgggaa atgtatctca cggaagattg tagcactgcg aaatgagaat
    19201 gggagctaca accggtttga acagcagtat atgggaagaa acattgtagc actgcgaaat
    19261 gagaatgggc gctacaacca gccgttgcga taagcgaacg tgcgttaaag tcattgagac
    19321 acagtgcaat tatggcaaga aaggcgacag atattgtgtc acagatttga aataagaaaa
    19381 aaccgcccac tcacagagta ggcggaaaac ttgcgttttt ttgattgtgg tcgggttagc
    19441 tactgcattg acttaacagc aaagcaaccg ctaaaatgaa cttgtggttc acttcaacgg
    19501 acagcagcaa ctttttccca cgcataagga atttaagcat tttagcttct cctagtgaag
    19561 ttaatcacac aggcgagagc cgaacaaaaa tccccgttgc agcggggatt tttgctgttc
    19621 cctgtttgat gattaaacta aaatcttgca aaatgagacc tttgcaaaat tccccaaaat
    19681 cccctaaatt cccaccaaga catttagggg atttctcatg agcaccttct ttcagcaaac
    19741 cgcccaagcc atgattgcta aacacatcga ccgtttccc
//
LOCUS       DAWHXS010000032        19778 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWHXS010000032 DAWHXS010000000
VERSION     DAWHXS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.032"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(35..250)
                     /gene="nqrM"
                     /locus_tag="WIE22_000860"
     CDS             complement(35..250)
                     /gene="nqrM"
                     /locus_tag="WIE22_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ4126972.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDH"
     gene            complement(276..1331)
                     /locus_tag="WIE22_000861"
     CDS             complement(276..1331)
                     /locus_tag="WIE22_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ4126973.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(1484..2701)
                     /gene="nqrF"
                     /locus_tag="WIE22_000862"
     CDS             complement(1484..2701)
                     /gene="nqrF"
                     /locus_tag="WIE22_000862"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ4126974.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVSVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(2715..3308)
                     /gene="nqrE"
                     /locus_tag="WIE22_000863"
     CDS             complement(2715..3308)
                     /gene="nqrE"
                     /locus_tag="WIE22_000863"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ4126975.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(3312..3938)
                     /locus_tag="WIE22_000864"
     CDS             complement(3312..3938)
                     /locus_tag="WIE22_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ4126976.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(3938..4714)
                     /locus_tag="WIE22_000865"
     CDS             complement(3938..4714)
                     /locus_tag="WIE22_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ4126977.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(4707..5939)
                     /locus_tag="WIE22_000866"
     CDS             complement(4707..5939)
                     /locus_tag="WIE22_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ4126978.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGVQAFGALTPDLLQQNIANDWHYTLAD
                     ALGINMSSEASVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(5942..7285)
                     /locus_tag="WIE22_000867"
     CDS             complement(5942..7285)
                     /locus_tag="WIE22_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ4126979.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAAPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(7634..9055)
                     /locus_tag="WIE22_000868"
     CDS             complement(7634..9055)
                     /locus_tag="WIE22_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ4126980.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(9415..9774)
                     /locus_tag="WIE22_000869"
     CDS             complement(9415..9774)
                     /locus_tag="WIE22_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ4126981.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFYRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(9771..10376)
                     /locus_tag="WIE22_000870"
     CDS             complement(9771..10376)
                     /locus_tag="WIE22_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ4126982.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(10423..10905)
                     /locus_tag="WIE22_000871"
     CDS             complement(10423..10905)
                     /locus_tag="WIE22_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ4126983.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      11109..11206
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11417..12517
                     /gene="gcvT"
                     /locus_tag="WIE22_000872"
     CDS             11417..12517
                     /gene="gcvT"
                     /locus_tag="WIE22_000872"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ4126984.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            12680..13066
                     /gene="gcvH"
                     /locus_tag="WIE22_000873"
     CDS             12680..13066
                     /gene="gcvH"
                     /locus_tag="WIE22_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ4126985.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            13218..14465
                     /locus_tag="WIE22_000874"
     CDS             13218..14465
                     /locus_tag="WIE22_000874"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ4126986.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEIRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            14741..15167
                     /locus_tag="WIE22_000875"
                     /pseudo
     CDS             14741..15167
                     /locus_tag="WIE22_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15173..16402
                     /locus_tag="WIE22_000876"
                     /pseudo
     CDS             15173..16402
                     /locus_tag="WIE22_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            16460..17080
                     /locus_tag="WIE22_000877"
     CDS             16460..17080
                     /locus_tag="WIE22_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4126987.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            complement(17112..17174)
                     /locus_tag="WIE22_000878"
                     /pseudo
     CDS             complement(17112..17174)
                     /locus_tag="WIE22_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            17170..17301
                     /locus_tag="WIE22_000879"
                     /pseudo
     CDS             17170..17301
                     /locus_tag="WIE22_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            17298..17792
                     /locus_tag="WIE22_000880"
     CDS             17298..17792
                     /locus_tag="WIE22_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ4126988.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTKWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKDGKVVSFDDMPDT
                     YIFK"
     gene            complement(17880..19541)
                     /locus_tag="WIE22_000881"
     CDS             complement(17880..19541)
                     /locus_tag="WIE22_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ4126989.1"
                     /translation="MTESITRDNMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTKKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAKQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVMHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGFQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYRELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHYGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         4769 a         4985 c         5366 g         4658 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatttc ggcgttaatg gtcaacccgt atgccgcctg
       61 cctgtttttc ttcatccagt ttcttttgca gggtgtcgca aggtgtgtcg cagtcgcaca
      121 tttttttcat acccaaggca gtaatgccgc cgcaactgcc tttgatgctg cgtttggaga
      181 aaatatagcc gaccgccata ccgatgatga cggtcaggaa gatgccgaag gtaaggagca
      241 gggttttcat ggtgtttcct aatcggtttg tatgtttagc ggagcagttt ttcaaattcg
      301 gaagacatgg cggtgcggta gccgccttta tccctgacaa tcaggaaaac agcgagtttt
      361 tcgcgctctg ccagctttaa ggcttcggtt tcgcccaata cgaataatcc tgtggacaag
      421 ccgtccgccg tcatcgcact gtctgcgacc acgctgatgg aggcgaggtt gtggctgatg
      481 ggtcgtttgt tgttcgggtt gatgatatgg gagaggcgtt tgccgttttt atcgacgtgg
      541 aaaatacggt aatcgccgga agtggcaagc gaacggttgt tcagcgggac gataatctgc
      601 gtattgccgc cttggacgat attgggctgc tcgataccga tgcgccacgg ttcgccgcgc
      661 gcgtttttgc ctttgccgtg caactcgccg ccgatttcga ccagataatt ttgaatgccg
      721 tatttttcca gttcgcccgc aactttatca acgccgaagc ctttggcaat cgaagataaa
      781 tccaaatagg ccttggggtg ggttttgctc aaggaagcgt aatctttgcc ttgtttcaaa
      841 atgattttgt ctatgcccgt ataagatgcc gcctgtttga tttgttccgg cgacggttca
      901 cgggtaacgg atttgtcggg gccgaatccc caaaggttga ccaaggggcc gacggttacg
      961 tccagcgcgc cgtgtgtcag gcggttcagg cggacggctt cggcagtaac gtgtgcgaag
     1021 tcgcttgaaa tgcggagggg cttgccggct gtgtgttggt tgaaccggct gatttcggag
     1081 tcgggctgat aggtggacat ctgccggttg acttctttaa gcgcgtcatc gatgcgtttt
     1141 tgtatttcgg caggtgaggg gagtttgtcc cgattatttg aaaggtattt gacggtatag
     1201 gtcgtgccca tcgtttcgcc ttgcagggta acggtttgcg cggtttgttc cgaacaggcg
     1261 ttcaggaaga tgaaacccag ggcaaatatc aagacgcgga taaagttcgg caggcgtgtt
     1321 tcagacggca tagtgtttga cggttttggc aaatggtttg aattatatcg caaaacggcc
     1381 ggtatgtttc tatgccgatg ccgtctgaag ggtgttcgga tggcatcggc atagaaaaag
     1441 gaagaaaccg aggtttcttc cttttgtatt tgaagccgaa tatttaaccg ccgaaatcgt
     1501 ccaagaggat gttttcgtct tccacgccca agtctttgag cattttgatg acggactggt
     1561 tcataatcgg agggccgcac atataaaatt cgcagtcttc cggtgcttcg tggtttttca
     1621 ggtggttttc gtaaaccacg ttgtgaatga agcccgtgta gccgtcccag ttgtcttccg
     1681 gcagcgggtc ggacagggcg acgtgccacg tgaagttcgg gaactctgcc gcgagttggt
     1741 caaagtcttc gacatagaac atctcgcgtt tggaacgtgc gccgtaccag aaggtaatct
     1801 tacgtttgga gttcaaacgt ttcaactggt cgaaaatgtg ggaacgcatc ggagccatac
     1861 ccgcaccgcc gccgataaat accatttcgg catcggtgtc tttggcgaaa aattcgccga
     1921 acgggccgga aatcgtaact ttgtcgccgg gtttgagcga ccagatgtag gacgacattt
     1981 gtcccggagg cgcatcaggt acgcgcgggg gcggcgtggc gatacgcacg ttcagcataa
     2041 tgatgccttt ttcttcagga tacgaagcca tagagtaggc acgcaaaatc ggctcgtcca
     2101 ctttggaaac gtattgccac aaattgtatt tgtcccagtc ttcgtgatat tccttaggaa
     2161 tgtcgaagtc tttgtaggca acagtgtgag gaggagcttc aatttgaatg tagccgccgg
     2221 cgcggaaggg gacttcttcg ccttcgggaa tggcaagctt gagttcttta atgaacgtgg
     2281 ctttgttatc gttggagatg acggtgcatt cccatttttt cacgccgaac acttcttcgg
     2341 gcacttcgat gtccatgtcg gttttgacgc tgacttggca cgacagacgg cagccttcgc
     2401 gtgcttcgcg tttgctgatg tgggacagct cggtcggcag gatgtcgccg ccgccgcttt
     2461 ttacgacgac gcggcattgt ccgcacgaac cgcccccgcc gcaggcggag gggataaaga
     2521 tgccttcgtt ggcaagcgcg cccaagagtt tgccgccggc gggcatcgtc agctcttttt
     2581 cgccgttgac tttgatggtg atgtcgcctt cgctgaccag tttggatttg gcaaatagaa
     2641 tcatcagtgc caaaaccaaa acgatgacgg taaacatcac gatacctaaa ataatctcca
     2701 taccggtccc tttcttataa ctggatgccg gagaacgaca taaacgccat cgccatcagg
     2761 ccggcggcga taaaggtaat gcccaagcct ttgaggcctt tgggagcgtc cgaatatttc
     2821 attttttcgg taatgcccgc caaagcgaca atcgccaaca tccagcccaa gcccgcgccg
     2881 aagccgtata caacggactc gccgaagttg tattcgcgtt gcgccataaa cgatacggcg
     2941 ccgaaaatcg cgcagttcac ggtaatcagc ggcaggtaga tgcccaacgc gttatagagg
     3001 gcggggacga atttatccaa gaacatttcc aaaatctgca ccaaagcggc aatcacgccg
     3061 atgaaggtga tgaatttcaa aaaggtcaaa tccacgcctt cgacaatcgc gccgtctttg
     3121 agcagcgagt aaacgagttg gttgacaggg acggacagcc cgagtacgaa aattaccgcc
     3181 acacccaaac cgaatgcggt ggatactttt ttggataccg ccaaaaacgt gcacataccc
     3241 aaaaagaagg atagtgccat attttcaatg aagacggatt tgatgaagag gctcaaatag
     3301 tgttccatag cttattcctc cgcctgttcg ggtttccagg tacgcagtcc ccaaatcaaa
     3361 aagccgatga tgaagaacgc gctgggggcg agcaggaaca agccgttggt ctgataccag
     3421 ccgccgtcct gcacggtttg gaaaacggtg tagcccaaga gtttgcccga gccgatcagt
     3481 tcgcggacgg tggcgacgac aagcagcatc atcccgtagc ccgcgccgtt gccgatgccg
     3541 tcgatcaggc tttccagcgg cggctctttc atcgcaaatg cttcggcgcg gcccatcacg
     3601 atacagttgg taataatcag accgacgaat acggaaagct gtttggacaa ttcgtaggca
     3661 aatgcctgca agagttggtc gaccagcgta accagcgacg cgataatcgc catttgcacg
     3721 ataatacgga tgctgttggg gatgtagttg cgtaccagcg aaatgaagaa gctggaaaaa
     3781 ccggttacca aagctacgga aatacccatc acgatggccg tctgaagttt ggtggtaacc
     3841 gccaaagccg aacaaatacc caaaacctgc aaggcaatcg ggttgttgtc gataaagggt
     3901 gaaaacatca aatgtttcaa gcgtttcata tcagccatta ttgcgctcct gctgatttca
     3961 atttgttcag gtaggggata tagccgtttt cgccgaacca gtaggcgaac gaaccttgca
     4021 cgcctttgga tgtcagcgat gcgccggaga gggcatctac gccgtgttct ttgtccgaac
     4081 ccgcgccttt gccgacgtgc agggcgagtt tgccttgtcc gtcaaacagt tttttgccga
     4141 cgaatttttg ctgccacaac ggattgccga tttcgccgcc caagcccggg gtttcgcctt
     4201 gttcgtagta ggtaatgccg ttgatggtgt tgccgtcggg ctggatggcg acaaagccgt
     4261 acatgaccga ccacaaaccg ttaccgtgca taggcaggat gatttgcccg attttgccgt
     4321 cttcgccttt taccaaataa acctcggtgt atttggcacg gcttttgatg cctgccaaat
     4381 cgtcttcggg tttgatgcgg atgctttggg cagggtcttt gcctgcgatg cgcgcgctga
     4441 agtctttagg cgcatcggcg acgtattcgc cggtcgccaa atcgacaaca cgttgctcga
     4501 tacgctcggc aaaggtttta ccgatgtcgg tgtccttatc catcaaaccg gctacgctca
     4561 agatatagcc ttgtttgtct tggagttttt gtttctcttg gatgggtttc aagccgacga
     4621 ccgcaccggc aacgatgacc gagcaaatca ggctgaccgc caacacgaca atcagcgtac
     4681 cgctgaagct gtctttatcg aatttcttag ccattgctgc gcgcctttct gcgtttgatg
     4741 ttcgcttgtg cgacgaaata gtcgaaaatc ggggcaaaca ggttggcaaa cagaatcgcc
     4801 aacatcatgc cttcggggta agccggattg accacgcgga ttaatacgca catcacaccg
     4861 atcagtgcgc cgtaccacca tttgccgaca ttggtaaagg aagcggaaac ggggtcggtc
     4921 gccataaaca gcataccgat ggcgaagccg ccgacgacca aatgccagta ccaaggcata
     4981 gcaaacatag cgttggtgtc cgaaccgatg aagttgaaca gcgaagacat cgcaatcata
     5041 ccgatcatca cgccggcaat aatgcgccaa gaagcgatgc gggcaaacac gataaacgcg
     5101 ccgccgatta agagtgccaa agtggagact tcgccgatgg agccgggcag tttgccgata
     5161 aacgcgtcca tccaagtgat ggtttgaccg gttacggcgt ttttcaggcc gtctgcaccg
     5221 tgtgccgccc attgcgccag cgcggttgcg ccggaatagc cgtcaaccgc cgtccaaacc
     5281 gcatcgccgc tcaagttggc ggggtaggcg aagaacagga aggcgcggcc tgccagcgca
     5341 gggttcataa agtttttacc cgtaccgccg aatacttctt tcgcaaccac aacgccgaaa
     5401 gaaataccca aagccgcctg ccacagcggc agcgtgggcg gaacgattaa ggcaaacaga
     5461 atcgaggtaa caaagaaacc ttcgttgatt tcgtgtttgc gcacggtggc gaacaagact
     5521 tcccagaaac cgccgacggc aaatacggtc gcgtaaatcg gcaggaagta aatcgcgccg
     5581 aacagcattt tgcccaacac gctcgcttca gacgacatat tgatgcccaa agcgtcggca
     5641 agggtgtaat gccagtcgtt ggcgatgttt tgttgcagca aatcaggcgt taacgcaccg
     5701 aatgcctgta cgccgacgtt gtacataccg tagaacatgg cagggaacaa agccagccac
     5761 accaaaatca tcatgcgttt ggagtcgagc gcatcgcgga cgtgtgccgc tttgcgcgtt
     5821 accgcaccgg atgtatagaa aatcgtcgcc gcagcttcgt agagggcata ccatttttca
     5881 tgtttgccgc ctggcaggaa gtgcggttcg attttttcca gaaaatgttt caagcccata
     5941 atcagccttc cttctcaatg gtttccagca ccttacgcaa cagcgggccg tattcgtatt
     6001 tgcccgggca gacgaagctg cacaaagcga ggtcttcttc gtccaattcc aagcaaccca
     6061 atgcctgcgc gctgtcggta tcgccgacga ttaaatcgcg caaaagcagg gtaggcagga
     6121 tgtctagcgg cattacgcgc tcgtaagtac caatcggcac catggcgcgg tcgccaccgt
     6181 tgacggctgt cgtgaacttg aagagtttgt ttttcaggaa atgaccgagg gtcgtacgcg
     6241 tgatcgagta tttgtccggt tgtggcgcaa cccagccgaa cagttcttta ctgcggcctt
     6301 cttcgataac ggaaatctga ttgtggtagc gtcccaaata atcgtgcgcg ccttgtgcaa
     6361 tcgcgccgtt caataccgaa ccggaaatca cgcggttgtc tgcgtcaacc aattcacccg
     6421 cagtaatttg cgatactttc gcacccaaaa cggtacgcaa gaggcgtggt ttgttgactt
     6481 gagaaccacc caaggcaacc acgcgctcgg tgttcagacg gcctgttgca aacaaacgtc
     6541 cgatggcaat tacatcttga taattgatgg tccaaacggt tttgttcgca ccgacaggct
     6601 caatgaaatg aatgtgcgtg ccactcaaac cggcaggatg cgggccgcca aattcatgag
     6661 tttcgatgtt ggcggcgttt tcagacggca cgtctgcgcc tgctgcttta cacacatgga
     6721 ttttacgctc ggtcaaacgg ctcaatacca gcaaacctcg tctgaaatcc tcggcggctt
     6781 ctttgatcac aaccacaggg tctgccgcca gcggattggt gtccatcgca ttgacgaaga
     6841 tggcgaacgg cgcggcatcg acggcaggga ttttgctgaa cggacgggta cgcagcgcag
     6901 tccacaaacc ggattggatc agattgcggc gcacttcttc gccgcttaag tttgccaacg
     6961 cttcgggtgc gtagcgttca aactcgattt cgtcgttgcc ttcaacggca atcacgactg
     7021 actgaagtac gcgcttttcg ccgcgatgga tggcggcgat tttgccagaa gccggcgcgg
     7081 taaacaccac gcccggattc tttttgtctt caaacagcac ttggcctttt ttgacggcat
     7141 cgccttcctt gactttcatc gaggggcgca taccggcata ttcttcgcca agcaacgcga
     7201 cttcggtaat ggccgggccg tcataaatga cttgctccgg tctgcccgcg atgggcaggt
     7261 ttagaccttt tttgatttta atcatatatt tgcattactt gtgatggtta aggtaaaaac
     7321 ggcgtgtttt gataccgtgt cgcgtggcat caaaagcatt gaataaatta atgtagcaaa
     7381 gtgttagatt ctatcaggaa ttgtacctgt ttgtcagatt tgctgctttt ttccttgcgg
     7441 aagccgtttt tatagtggat taaatttaaa ccagtacggc gttgcctcgc cttgccgtac
     7501 tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataattg
     7561 tcggaaagga aaaatattga tttgattatg ccggaattta aaatgccgtc tgaatgttca
     7621 gacggcatag cgtttacagc agtttgaaaa cgaaaaagat aagggtatgt acgatgaaga
     7681 cgggtgtcag gaaggcgacc gaccacatca tatagccgaa gaaagtcggc atcggtacgc
     7741 cgcgctgttc ggcaatggcc ttgaccatga agttcggtgc gttgccgatg taggtcagtg
     7801 cgcccatgaa taccgaaccc atagaaaccg ccagcagcga atgaaacagg gtacccgtca
     7861 tcaaggcttg ggcatcgccg cctgccatat tgaaaaaaac gagataagtg ggcgcgttat
     7921 ccaagaatgc cgacaatatg ccgctcatcc aaaaatacat cacattaatc ggatgacctg
     7981 ccgtatcgtg aaccagcgat accaccccgc ccagcgcgcc tgcctcgcct gctttcagaa
     8041 tgctcaggac gggaaagatg gtgatgaaga tgccgaggaa gagtttgccc acttcggcga
     8101 tgggttcaaa gttgaattcg ttgcctgcgc ggacttgttt gggcgtgatt gccatagata
     8161 cggcggtcaa tgcaatcagg atgacatcgc ggacgaggtt ttgcagggcg taacggctgc
     8221 cgaggatttc aaatcccggg tgttcgggtt tccaaaggcc ggacattaga accgcgccga
     8281 ccacgcccga aagcaggagg aagttccatt tgccgaagat ggcgattttt tcgggttttt
     8341 cctgttgtgc cggcgtatct tgtgcaatgc tttcctgttt gaagaaacgg ttgtcgatga
     8401 aatagaaggc ggtcaacagg acagcggtgc tgatcaggac gggggcgaac atatgtttga
     8461 ccgtccacat gaaatctacg cctttgagga agccgaggaa gagtgggggg tcgcccaaag
     8521 gggtcagacc gccgccgatg tttgcaacca ggaaaatgaa gaagatgacg atgtgcacgc
     8581 ggcgggtacg gttttggttg gctttcagca gcggacgaat catcagcatt gctgcgccgg
     8641 tcgttcccat gatagaggca agtgccgtac cgacggcaag cagggcggtg ttgagcttgg
     8701 gtgtgccgtt caagtcgccc caaaccaaaa tgccgcctga aatggtgtac agggcaagca
     8761 gcagcaggat gaaagggatg tattcttcaa cgagtgcgtg tgcgacggta tggataccgg
     8821 cggacgcgcc aaaaaccaaa ctgaacggga tgaggaagag caatgtccaa aaggcggtaa
     8881 ttttgccgta atggtgatgc caggtatgcg aaaaaaacaa gggacccaat gcgatagaca
     8941 gcaaaatcag ggcaaagggc aggccccaca gcaggtttag gtttgcgccg tccaaatctg
     9001 cggcgtaaac cgatgctggg aaaagcatta gtgaaaacag gggtaggtgg cgcatcgtgt
     9061 ttcctcgatt caagcactgc cttgcgcggc gcgtgggagt gatacaggca ccgtgccgcc
     9121 cggacatagg cggactgtgc tgcctgtttg tttttgaaaa aagttttccg attgattgta
     9181 aagtaaataa tcaggataaa ccagtttccc aaaccggaag gcggcgggaa ggcggattgc
     9241 tgtgcttggg aatatatgtt ctttttgata aataatttta tttaaaacaa ataaatatat
     9301 gtctttaata aataatctat cgaaaacgaa aaatgaattt attttaacat atatttgcaa
     9361 tgaaacaggt ttgccccccc cgtttgtttg cccttatccc tttcagtacg gcattcaaga
     9421 ttcgggcctg cgccacatcc atatggcgac aagggaacaa aaaaccgatg aaaccgcccc
     9481 gacccaccag cgttggggaa actgccaaaa cattatcagg caggatgcgg tcatcatact
     9541 gatggcgaat attttggctt tgcgcggcac tgcgccgttt tgttcccagt tatgaaccat
     9601 cgggccgaaa tagcggtgcc ggtgcagcca gcggtaaaag cgcggggatg cctttgccca
     9661 gcaggcggcg gagagcagta cgaacggcgt ggtcggcaac agcggcaaaa aaatgccgat
     9721 gatacccaac agtagggaaa tgcagccgca ggcaattaaa agataacgta tcattttgaa
     9781 atatttttct tattgtgcgg ataagggcag gatgtgatac cgagttttgc ccagccttca
     9841 tgtcccattt tttccagcag ggcgatattg cgttcgaata ttgccgaagc gtcgggaaag
     9901 gcttgtgcgg ctttggcaat gctgtcttcg cggatgaggt gcagcgtcgg atagggagaa
     9961 cggttggtgt agttgccaat gtcgtctgaa tccgtgcctt caaattggaa atcgggatga
    10021 aacggggcga tttggacgat gccttctaag ccgttttcga caacggcggc atcggcaatg
    10081 tcgagcatat cgttgaatac gtcgaaatcg gggaataggg tcgggtgaac cagcagggtg
    10141 gtttccagtt cggtggcggg tgtattgccc agtcgctgca gttcttcgtc caagtcttcc
    10201 aaaaaaccgt caaggtgttt ggcttcgctg atcgcgatgc ggacaaggtt tttaacgtgg
    10261 ggggctttgg caaagggaca caggttcaga ccgatgacgg ctttttccaa ccattgtccg
    10321 gtgtgttcgg caacagcatc tttattttcg gaagtattga tattcattat tgtcatgtaa
    10381 atgtgtttgc agattgcacg tgtgggaaaa tcgggaaggg cactattcct tcagcaggtg
    10441 gttgagcggt agggaggtgg tgtgtttgat ttcttttaaa acaaagctcg attgcgcatc
    10501 ttgtacgccg tggtgggaca ggagcgtatc caaaacaaaa tgggaaaacg cgttcatatc
    10561 ggtaaaaaac gcctgaagca ggtagtcggt ttcccctgtc agggcgaagc agctcaagac
    10621 ttccggccat tttcggaccg atgcggcaaa gtcttcccgc gcgtcttttg ctttgcggat
    10681 ggaaacgcgg ataaatgcct gaagccccag gttgacggat tccggagaca gcagcgcggc
    10741 atattggcgg acgatgccgg catcttccaa ctgcttcaga cggcgcaggc acggagaagg
    10801 cgaaagtgcg acacgttcgg acagttcgac attggtcagc ctgccgtttt cctggagaac
    10861 ctgtaagatt ttaatatcgg ttttgtctaa agtgagttgg ggcatatttg cgttccgttt
    10921 taaggaattc ggattgtctg tccgtatgtt tgcggcaatc cgcacagatg gagaccatat
    10981 taacatataa aaagttatac cgtcatccgg gacaaatttt gttttcggaa aatcatgtga
    11041 aaacagaggc ggtcggtttg catctctttt aagacggctt gcccaaaccg ccgattcaag
    11101 acataatcgg gaaatgtgca ggagagtgtt acacccaact acaatgtaac caccgaaggc
    11161 gcagacaccc ttaaatcgct caggtatcag ggactgcaca ttgaaacaaa caatctggag
    11221 agcggcgttg gaataacgtc caccgaaggg gagaaggccg tctgaaccac cattcagaca
    11281 accgcgcaaa gcagtgagca gactggtttg ccatcatgcg gatacggccg aaaatctcag
    11341 gttcaaggac agatagggtc atccgcgcac aggtgcgcgg gtggcatctg aacaaaaaat
    11401 ccggagaaac ttgagaatga ttactctgaa aaccacccca tttcatcaag cccatcaaga
    11461 tgcaggcgcg aagccggtag attttgccgg ctgggagctg cccatccatt atggttcgca
    11521 aatcgccgaa cacgaagccg tgcgcaccga cgccggtatg tttgacgtat cccatatgct
    11581 tgttaccgac gtagcaggcg caaatgccaa agcctttttc cgcaaattga ttgccaacga
    11641 tgtcgccaag ctcgcttttg tcggcaaagc cctttattcc gctttgctca acgacaacgg
    11701 cggcgtgatt gacgacttga tcgtttaccg caccaacgaa gccgaaaccc aataccgcat
    11761 cgtgtccaac ggcgcgactc gcgaaaaaga tacggcacaa ttccacaaag tcggacaaga
    11821 gttcggcgtt gcctttaatc cgcgctacga cctcggcatg ctcgccgtac aaggcccgaa
    11881 agccatcgaa aaactcctga ccgtcaaacc tgaatgggcg gatgtgattc acggcctcaa
    11941 accgttccaa ggtgcagact tgggcaacga ttggtttgtc gcccgcaccg gctataccgg
    12001 cgaagacggc gtcgaagtga ttctgccggg cactgaagcc gtcgcattct tcaaagccct
    12061 gcaagcagca ggcgtacaac cttgcggtct cggcgcgcgc gacaccctgc gcatggaagc
    12121 cggcatgaac ctctacggca acgatatgga cgacgacacc agcccgctcg aagcaggcat
    12181 gggttggacc gttgatttga aagacgaaag ccgcgatttt gtcggcaaag ctgccttgtt
    12241 ggcattgaaa gaaaaaggcg ttgccgtcaa acaggtcggt ttgctgctcg gcaaaggcgg
    12301 catcctgcgc gcgcatatgg aagtgttgac cgacaaaggc aaaggcgaaa ccaccagcgg
    12361 cgtattctcg cccagcctga aacaatccat cgccatcgcc cgcgtaccga aagactttga
    12421 cggcgatacc gctaaagtgc tgatgcgtgg caaggaagtg gacgtgcgcg tattgaagtt
    12481 gccgttcgtg cgtaacggtc aaaaacagtt tgattaatat cggttcagac ggcattttca
    12541 tttcatatgc cgtctgaaag caggttttaa ttgtcgtccg atacggatat ttgtagaaag
    12601 cattgaacaa ggcatctgtg gatattgatt catgcagatg ccgtctgaaa ataaccccct
    12661 accaatggag tatcaaacca tgagcaacaa catccccgcc gaactgaaat acgttgccag
    12721 ccacgaatgg ctgcgccttg aagaagacgg tacgattacc gtcggcatta cccaccacgc
    12781 gcaagagctg ttgggcgaca tcgtgttcgt cgagctgccc gaagtcggcg caaatttggc
    12841 tgccgaagaa caggccggtg tggttgagtc tgtaaaagcc gcgtccgacg tgtacgcacc
    12901 gattgcaggt gaagtcgttg ccgtcaacga agatttgcca agcgctccgg aaactgccaa
    12961 cagcgatcct tacggtgcag gctggttctt caaactcaaa ccggcaaacg ttgccgatta
    13021 cgacgttctg ctgactgccg aacaatacgc gggcgaagtg gattaaaccg cccggctgcc
    13081 cgatggcaac cgccggacaa acggaaactg caccttcaga cggcattttt gcggtcggag
    13141 gtgcagtttt ttgtccgtgt tttaaggaag cagttaggct ataataacgg tctatattca
    13201 tctttaccga ttttttcatg caacttaccg ctgtcggact caatcatcaa accgcacctt
    13261 taagcatacg ggaaaagctg gcgtttgccg cggcctgcct gcccgaagcc gtccgcaatc
    13321 ttgcccgaag caatgcggca acggaggcgg taatcctttc tacctgcaac cgtaccgagc
    13381 tttactgtgt aggtgattcg gaagaaatca tccgttggct cgcagactat cacagccttc
    13441 ccatagaaga aatcagcccc tacctttata ctttggggat gcaggagact gtgcgccatg
    13501 ctttccgcgt cgcctgcggc ttggattcga tggtgttggg cgagccgcag attttaggac
    13561 agattaagga tgcggtcagg gttgctcaag agcaggaaag tatgggtaag aaactcaatg
    13621 ccctgttcca aaaaaccttt tccgttgcta aagagatccg taccgatacc gctgtcggcg
    13681 agaactcggt ttccatggct tccgcttccg tcaagttggc agagcagatt ttccccgaca
    13741 tcggcgattt gaatgtcttg tttatcggtg cgggtgagat gattgagctg gttgccactt
    13801 attttgccgc caaaagtccc cggctgatga cggttgccaa ccggacgctg gcgcgtgcac
    13861 aggagttgtg cgacaagctc ggtgtcaacg ccgaaccgtg cctgctgtcc gatctgcctg
    13921 ccattctgca cgattacgat gtggtggttt cttcaacggc gagccagctt ccgatagtcg
    13981 gcaaaggcat ggtggagcgt gcattgaaac aaaggcagag tatgccgttg ttcatgcttg
    14041 atttggccgt gccgcgtgac attgaggcgg aagtcggcga tttgaacgat gcctatcttt
    14101 atacggtgga cgatatggtc aatatcgtcc aaagcggcaa ggaggcaagg cagaaggccg
    14161 ccgccgccgc cgaaacgctg gtgtccgaga aggttgccga atttgtcagg cagcagcagg
    14221 gcaggcagag cgtccccttg attaaagcct tgcgggacga gggcgagaaa gcgcgtaagc
    14281 aggtgttgga aaatgcgatg aaacagcttg ccaaaggcgc aacggcagaa gaggttttgg
    14341 aacggctgtc cgtccaactg accaacaagc tgctgcattc gccgacccaa actttgaata
    14401 aggcggggga agaagataaa gatttggttc atgccgtcgc gcagatttat catttggaca
    14461 aataacggtg cgccggaaaa ggatgccgtc tgaaggggtt tcagacggca ttttttgtgc
    14521 aggccggcaa catcgtgaaa tcccacatta tatcgatgta atcacaaagt atagtggatt
    14581 aacaaaaatc aggacaaggc gacaaagccg cagacagtac agatagtacg gcaaggcgag
    14641 gcaacgctgt actggtttaa atttaatcca ctatattatc ccgtatgcgg attggtttta
    14701 agatttgtaa atttgatttg catcaaaaaa tcgccgatag atgattcata taatatcaat
    14761 attaaagagt atcggtatat cggggatagt catgtcctgt ttttcaatca aacgtatgtc
    14821 cgcgtttcgg gcgcggataa cggcgttttt ttaccgcctt tgtctttttg acggcggcac
    14881 tgcccgctta tgcggagcgt ctgcctgatt ttctggcgaa aatacagcct tcggaaattt
    14941 ttccgggtgc ggaccgttac ggcaagccgg aaggcaagcc tatggttgcc cgcgtttaca
    15001 aaggcgatga gcagttgggc ttggtctata tcacgaccga tgcggtcaat acgcgcggtt
    15061 attcgagcaa accgatcgat acgctgatgg ctttggcaaa tgacggcacg atagccggag
    15121 cgaaactggt cgatcatcac gaaccgatta tgctgatcgg tatcccgcat ttgcccgcgc
    15181 ccgggcgggc gatacgctta aactggcttc cggcgtatat caaaccaaac tttatatcga
    15241 caaaccgatt acgattgaag ggcctgccga ccgttccgca accatcgaag gggacaggag
    15301 cggccgcacc atagccgtac acgcgccgga cgtaacgctc cgcaacctga ccgttacccg
    15361 ttccggtatg agcctgccag caatggatgc cggtatttat ctcgaagaaa ccgccccgcg
    15421 cgccctgatt gaacacaata atattttgga taattcggtc ggcgtctatc tgcatggttc
    15481 tgccgatgcg atggtgcggg agaataaaat cgtcggcgac gcgactttgc gcgtgaacga
    15541 gcgcggcaac ggcgttaccg tttggaacgc acccggtgcg caggtcgtcg gcaacgacat
    15601 ttccaaaggg cgggacggca ttttttccaa caccagcacg cacaacacct ataaaaacaa
    15661 ccgcttcagc gatttgcgtt tcgccgtcca ctatatgtac accaacgaca gcgaaatcag
    15721 cggcaatatt tccgtgggca acaatatggg ctatgtgctg atgttttccg agcggctcaa
    15781 agtgtttgac aatatcgccg tcggcagccg cgaccaaggc atcatgctca actatgtcaa
    15841 ctattccgat attcacgaca acattatcaa caaagcgggc aagtgcgttt ttgcctacaa
    15901 tgccaactac gataaactgt ccgccaatca ttttgaaaac tgccaaatcg gcatacactt
    15961 taccgccgcc atcgaaggca cgtccctgca cgacaattcc tttatcaaca acgaaagcca
    16021 ggtcaaatac gtcagcacgc gctttctcga ctggagcgag ggcggacacg gcaactattg
    16081 gagcgacaac agtgcgttcg atttgaacgg cgacggcttc ggcgacagcg cgtaccggcc
    16141 caacggcatc atcgaccaaa tcatctggcg cgcgcccgta tcgcgcctct tgatgaacag
    16201 tcccgccatc agcatcgtca aatgggcgca ggcgcaattt cccgccgttt tgcccggagg
    16261 cgtggtggac agcaaaccgc tgatgaagcc ttatgccccc aaaattcaaa ccagttatca
    16321 ggcgatgaag gacgagttgc tcaaagaagc cgaaacgcgg cagtcggaat ggggcagggc
    16381 ggaaaacggt tctttgaact agtctgcttc agacggcatc cggattcaaa tgccgtctga
    16441 aaacacaaaa ggaacaacca tgaccacaca tcatgtcgaa ttgaggaagg taaccaaacg
    16501 cttcggggcg caaaaagccg ttaaccaagt cgatttggtt ttgaaggcag gagaaagcgt
    16561 cgggcttgcc ggacacaacg gcgcgggcaa gtccaccatt atgaagctga tactcgggct
    16621 gattaccccg accgaaggcg aagtgatgct tttgggcgaa cgtaccggca gcaaagcggg
    16681 ggcgcggctt cgcagccaaa tcggctacct gcccgaaact gttgcgttgc acccttcgct
    16741 gaccggcatc gaaacgctgg atttttatgc caaacttaaa aaacagccgc tcacgcagaa
    16801 tcgggggctg ctcgagcgcg tcggcatttc acaggcggca caccgccgcg tcggcactta
    16861 ttctaaaggg atgcgccaac gccttgcctt ggcacaagcc ctgctgggcg agcccaaagt
    16921 cctgctgttt gacgaaccga caaccggcct cgaccctgca tcaaggcaaa tgttttacga
    16981 agtcgtgcgc gaactcaacg ggcgcggcgc gaccgtattg ctcagtaccc acgcccttgc
    17041 cgagttggac ggacacgccg accgcattat cgtggattaa atttaaacca gtacggcgtt
    17101 acctcgcctt accgtactat ttgtactgtc tgcggcttcg ttgccttgtc ctgatttaaa
    17161 tttaatccac tatatgcggg tatggcgggt ttgagcggac aaatcagcct gaccgtcccc
    17221 gttttgctga ccgctcaggt tttatgggtt atcattccgc ttgttttggc agccggaatt
    17281 tttagaaagc gacaaatatg aaaaaaaccc tgttggcaat tgttgccgtt tccgccttaa
    17341 gtgcctgccg gcaggcggaa gaggggccgc cgcctttacc ccggcagatt agcgaccgtt
    17401 cggtcggaca ctattgcagt atgaacctga ccgaacacaa cggccccaaa gcccagattt
    17461 tcttgaacgg caaacccgat cagcccgttt ggttctccac catcaagcag atgttcggct
    17521 ataccaagct gcccgaagag cctaaaggca tccgcgtgat ttacgttacc gatatgggta
    17581 atgttaccga ttggacaaat cccaatgccg acacgaagtg gatagatgcg aaaaaagcct
    17641 tttatgtcat cgacagcggc tttatcggcg gtatgggtgc ggaagacgca ctgccgttcg
    17701 gcaacaaaga gcaggctgag aaatttgcaa aggataaaga cggtaaggtt gtcagtttcg
    17761 acgatatgcc tgatacctat attttcaaat aatattgaaa aaaccggccg aaactttaaa
    17821 aacgagtttc ggtcggtttt tcttttcttg ttcggtatag tgtcggcagg aaagaacctt
    17881 cacatcccgc cgtaattcgg cccgctcgcg ccttcggggc aaatccaagt gatgttttgc
    17941 gtcgggtctt tgatgtcgca ggttttgcag tgcacgcagt ttgccgcgtt gatttgcagg
    18001 cgcggattgc cgttttcttc aacaatttcg tacacgccgg ccggacaata gcgcgtttcg
    18061 ggcgaggcgt attctttgta gttcacgtct atcatcgttt gcggattgtt cagcaccaaa
    18121 tggtcgggct ggttttcttc gtgcgcgaga ttggcaagga agacgctgct caagcggtcg
    18181 aaggtcaaca cgccgtcggg tttcggataa tcaatcggct tgcacgcggc ggcttttttg
    18241 agctgctcgt tgtctttgcc gtaatgtttc aaggtccacg gggctttgcc tctgaaaatc
    18301 atctggtcga tgccggtata gattgagccg aggtaaacgc cccatttgaa tgacggacgg
    18361 acattgcgcg cggcgtaaag ctctcgatac agccagcttt gttcaaaacg ttgctgataa
    18421 tccgccgctt ctttgccgct gtcgaaaccc tccacttctt caaggttttc caacaagggg
    18481 aacacggctt cggcggcgag catggcggat ttcatcgcgg tatgaatgcc tttgatgcgc
    18541 ggcatattga ggaaacccgc cgcatcgccg accaaaacgc cgcctttgaa cgagagcttc
    18601 ggcaaacttt gaaaaccgcc ttcaatcagc gaacgcgcac cgtaagcaat gcggcggccg
    18661 ccttcaaagg ttttgcggat ttcgggatgg gttttgaaac gttggaactc ttcaaacggc
    18721 gacagataag gattttgata gtccaaaccg accacgaagc cgacggcaac tttgttgtcg
    18781 tcgaaatggt aaacaaacgc gccgccgtag gttttgctgt ccagcggcca gcctgcgctg
    18841 tgcatcacca aaccgggctg atgctgttcg gacggcactt cccaaacttc tttaatgccc
    18901 aagccgtaag tttgcggctg gctgttttgg tcgagtttga agcgttcgat gatttgcttg
    18961 gaaagcgaac ctcggcaacc ttcggcaaac agggtttgct gtgcccaaag ctccatgccg
    19021 ggctggaatg aatcggtcgg ttcgccgtct ttgcccacgc ccatattgcc ggttgcaatg
    19081 cctttgaccg aaccgtcttt gtgatacagc acttcggcag cggcaaagcc cggataaatt
    19141 tccacgccca aattttccgc ctgcttcgcc aaccagcgta cgacttcgcc caagctgacg
    19201 atgtagttgc cgtgattgtc gaaattcggg gtaataggca gattgaacgc ctttttctta
    19261 gtcaggaaca atactttgtc ctgcgttacc gtacgcgtca gcggcgcgcc tttttctttc
    19321 cagtcgggaa tcaactcatt cagcgcaatc ggatcgataa ccgcgcccgc cagcgaatgt
    19381 gcacccgcct ccgaaccttt ctccaccacg caaacgctga tttcgcgccc attttgttcg
    19441 gcaagctgct tgagtttgat ggcggcggac aaacccgacg ggcctgcgcc gacaatcacg
    19501 acatcgtatt gcatattgtc gcgggtgatg gattctgtca tggcggttcc tgtgtattta
    19561 ttattgaatt gcaaatccgt aattatacaa cgggaacata tagttaccaa atacaacaaa
    19621 ggtcgtctga aaaccatatt ttcggttttc aggcgacctt tgtcgaaatt tcaataagca
    19681 cgccaccatt ttacctgtcc gaccgcaaac tccgtctgac gtttcggact gcgtgtgaaa
    19741 aacgccttat ccccgccggc atccctccct ttcggcac
//
LOCUS       DAWHXS010000033        19769 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWHXS010000033 DAWHXS010000000
VERSION     DAWHXS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.033"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            30..917
                     /gene="prmA"
                     /locus_tag="WIE22_000882"
     CDS             30..917
                     /gene="prmA"
                     /locus_tag="WIE22_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ4126990.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            935..1498
                     /gene="orn"
                     /locus_tag="WIE22_000883"
     CDS             935..1498
                     /gene="orn"
                     /locus_tag="WIE22_000883"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ4126991.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            1509..1781
                     /locus_tag="WIE22_000884"
     CDS             1509..1781
                     /locus_tag="WIE22_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ4126992.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(1997..3280)
                     /gene="hemL"
                     /locus_tag="WIE22_000885"
     CDS             complement(1997..3280)
                     /gene="hemL"
                     /locus_tag="WIE22_000885"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ4126993.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIVIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFTADSVGGMFGLYFASHTPQNYGDMARSNIEGFKQFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(3344..3517)
                     /locus_tag="WIE22_000886"
     CDS             complement(3344..3517)
                     /locus_tag="WIE22_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ4126994.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            complement(3830..3910)
                     /locus_tag="WIE22_000887"
                     /pseudo
     CDS             complement(3830..3910)
                     /locus_tag="WIE22_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            4113..5441
                     /gene="miaB"
                     /locus_tag="WIE22_000888"
     CDS             4113..5441
                     /gene="miaB"
                     /locus_tag="WIE22_000888"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ4126995.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(5526..7439)
                     /gene="dxs"
                     /locus_tag="WIE22_000889"
     CDS             complement(5526..7439)
                     /gene="dxs"
                     /locus_tag="WIE22_000889"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ4126996.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLEDLR
                     GRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(7524..8441)
                     /gene="xerC"
                     /locus_tag="WIE22_000890"
     CDS             complement(7524..8441)
                     /gene="xerC"
                     /locus_tag="WIE22_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ4126997.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            8635..9699
                     /locus_tag="WIE22_000891"
     CDS             8635..9699
                     /locus_tag="WIE22_000891"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ4126998.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVGAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            9719..10678
                     /locus_tag="WIE22_000892"
     CDS             9719..10678
                     /locus_tag="WIE22_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ4126999.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTTPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQIYKQNHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPSGKAEYHG
                     KAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKADEKSHAVILGDT
                     RYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            10817..11494
                     /gene="tsaB"
                     /locus_tag="WIE22_000893"
     CDS             10817..11494
                     /gene="tsaB"
                     /locus_tag="WIE22_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ4127000.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETLLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            11491..11931
                     /gene="rimI"
                     /locus_tag="WIE22_000894"
     CDS             11491..11931
                     /gene="rimI"
                     /locus_tag="WIE22_000894"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ4127001.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            11925..12662
                     /locus_tag="WIE22_000895"
     CDS             11925..12662
                     /locus_tag="WIE22_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ4127002.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            12724..13365
                     /gene="pyrE"
                     /locus_tag="WIE22_000896"
     CDS             12724..13365
                     /gene="pyrE"
                     /locus_tag="WIE22_000896"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ4127003.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            13439..13873
                     /locus_tag="WIE22_000897"
     CDS             13439..13873
                     /locus_tag="WIE22_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127004.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            13870..15180
                     /gene="argA"
                     /locus_tag="WIE22_000898"
     CDS             13870..15180
                     /gene="argA"
                     /locus_tag="WIE22_000898"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ4127005.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCATLKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            15379..17394
                     /locus_tag="WIE22_000899"
     CDS             15379..17394
                     /locus_tag="WIE22_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ4127006.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCAAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPDRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKCRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(17508..18464)
                     /locus_tag="WIE22_000900"
     CDS             complement(17508..18464)
                     /locus_tag="WIE22_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4127007.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            18700..19665
                     /locus_tag="WIE22_000901"
     CDS             18700..19665
                     /locus_tag="WIE22_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4127008.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         4477 a         5960 c         5347 g         3985 t
ORIGIN      
        1 aatccactat atttccaaga aagcccgtta tgccctacca acaaatcacc gtcaacgtca
       61 acgatgccgt cgccgaacgc ctcgccgacg cgctgatgga acacggcgca ctctccgccg
      121 ccatcgaaga tgcctacgcc ggcacgcaaa acgaacaggc gattttcggc gaacccggta
      181 tgcccgccga acaaatctgg cagcagagca aagtcatcgc cctgttcggc gaacacgacg
      241 aagccgccgc catcatccaa atcgccgcac aagaatgcgg gttaaaagac ttggcataca
      301 ccggcgaaac catcgaagac caagactggg tgcgtctcac gcaatcgcaa ttcgacccca
      361 tccgaatttc cgaccgcctg tggattaccc cctcttggca cgaagccccc gaaggcactg
      421 ccgtcaacct acgcctcgat cccggcctcg ccttcggcac tggcagccac ccaaccacgc
      481 gcctctgcct caaatggctg gatacgcaac tcaaaaacgg cgaaagcgtc ctcgactacg
      541 gctgcggttc gggcatcctg accatcgctg ccctcaaact cggcgcaggt tctgccgtcg
      601 gcgtcgatat tgacgaacaa gccgtccgcg ccggcaagga caacgccgaa cagaacaacg
      661 tcgatgcaca attcttcctg cccgacggcc tgcctcaagg gcaattcgac gtagttgttg
      721 ccaacatcct cgccaaccct ttacgtatgc tcggcgaaat gcttgccgcc cgcaccaaac
      781 aaggcggccg catcgtgtta tccggtttgt tggacgaaca aatcgaagaa ctcagcggca
      841 tttacagcca atggttcgat ctcgatcctg cggaaaccga agaaggatgg gcgcgattaa
      901 gcgggacaaa acgctgacaa gcaaaggaaa catcatgcag gataaaaaca acctatgctg
      961 gctcgatatg gaaatgacgg ggcttaatcc cgaaaccgac cgcattatcg aagtcgcgat
     1021 gattattacc gactcggatt tgaatgtatt ggcgcaatcc gaagtttacg ccatccacca
     1081 aagcgacgag ctgctcgaca atatggacga gtggaacacc gccacacacg gtaggacggg
     1141 gctgacgcag cgcgtacgcg aatcgtcgca taccgaagcc gaagtcgaac agaaactgct
     1201 ggactttatg tcggaatggg tacccgaacg cgccacgccg atgtgcggca actccatcca
     1261 ccaagaccgg cgttttatgg tcaaatatat gccgaaactg gaaaactact tccactaccg
     1321 caacctcgac gtttccacgc tcaaagagct tgccaaacgc tggaatccgc ccgttgccaa
     1381 aagcgtggtc aaacgcggtt cgcacaaggc attggacgac attttggaga gcatcgaaga
     1441 aatgcgccac taccgcgaac actttctgat ttccgccccg agagccgaag cgcaataaga
     1501 aacaaacaat gccgtctgaa gcgcaaatcc tgcatttcag acggcatttt tacagcagat
     1561 tgaaatcaaa aatatacacg cccgtcattc ccgcgcaggc gggaatccgg aaggtcgggc
     1621 ttgttggtca tgaagtggtc ttgaaaatga aacgttatcg aaaatctgac gaactgaaag
     1681 aaaaatatag cgatatagat gagacttttg aatttataaa ggggtttatc tatgtaattg
     1741 cccttgcttt taccgcatat gtcgctatcc gttttttctg atgcgcttcc gataaaatgc
     1801 cgtctgaagg cttcagacgg cattgtttca ggtggcggcg gcttcctgcc gggtgcggct
     1861 gatttcggca cgttattttc aatcattaca gaaactgaaa ggtctggatt cccgcctgcg
     1921 cgggaatgac ggcggtgtgc attcttactt caatctgcca aacaaattga acagggaaac
     1981 cctgtccgtc aaaacatcat gcagccatcg ccttgaacac ttcaaccgca accgcaaccg
     2041 tttcatcaat cagctcgggc gtatgcgcgg cagaaacgaa acctgcttca taagcggacg
     2101 ggccgaaggc aatgccgcgg tcgagcatgc cgtggaaaaa ctgtttgaag ccttcaatat
     2161 tggaacgcgc catgtcgccg tagttttgcg gcgtatggct ggcaaaatac aaaccaaaca
     2221 taccgcccac gctgtcggcg gtgaactcga tgcccgccgc atccgctgcc gtctgaaaac
     2281 cttgaaccaa ctgttcggta cgcgccgtca ggttttcata aaacccttcg cggcggatga
     2341 tttccagcgt tttcaaaccg gcggcgacgg caatcgggtt gcctgataat gtacctgcct
     2401 gatacacgcc gcccagtggg gaaatacatt ccataatgtc tttgcgtccg ccgaacgcgg
     2461 caagcggcat accgccgccg atgactttgc ccatcgtggt caggtcgggc gtgatgccgt
     2521 gcagcgattg tgcgccaccg agtgcgacgc ggaaacctgt catcacttcg tcgtaaatca
     2581 acaccgcgcc gtgtttttca gtcaattcgc gcagggcttt gacgaaggct tcggtcgggc
     2641 ggacaaggtt catattgccg acaaaaggct ccaaaatcac acaggcaatg tcattgccgc
     2701 tttgagcaaa ggcttcttcc aattgggcga tgttgttgta ttcgaggact aaggtgtgtt
     2761 tggtaaagtc ggcaggcaca ccggcggaag aaggattgcc gaacgtcagc aggccgctgc
     2821 cggctttcac cagcaggctg tcggaatgcc cgtggtagca gccttcaaac ttgatgattt
     2881 tgtcgcggcc ggtaaaaccg cgtgccagac ggatggcggt catcgtcgct tccgtgccgg
     2941 agctgacgag gcgcagccgt tcgacagacg gcataatttc ggcgatttgt tcggcgatga
     3001 cgatttcgcc ctcggtgggc gcaccgaatg acaaaccgcc caacgcggct tcgcgcacgg
     3061 cttcgacgac ttcggggtgc gcgtgtccga caatcgcagg gccccaagag ccgacatagt
     3121 cggtgtagcg cgtgccgttt tcatcccaaa catacgcgcc ttcggctttt ttgatgaagc
     3181 gcggtacgcc gccgacgctg ccgaatgcgc gcacggggga attcacgccg ccggggatga
     3241 ccgccttggc gcggtcgaat aaaatttcgt tacggttcat atttatcctc aaatgccgtc
     3301 tgaacggcag gtttcgggct tagaagcaga aagtcccatt ttatcatttt tcaggttgcg
     3361 acaaggattt gcctgcttct ttgcggatga cgccaaccgc atcccggatg acggaacgct
     3421 cgtctttttc cactttatgt gtaaagcggt agtcccggac aactccttcc cagtcgtaat
     3481 ccacacacca ctcccaatgt cggcgttctg atttcatata aatgaaattg gtcggcaaaa
     3541 aattataaat cggcaggctg acctcgtgat aggcataaca accgaaaggg ttgcgcttcc
     3601 cgaaacgtgc ctctacctcc gcccgggtcg ttttgccttt aacaaccgtt tgtgcgattc
     3661 cctcttccgt ctgatatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
     3721 agtacaaata gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct
     3781 ttgagctaag gcgaggcaac gctgtactgg tttttgttaa tccactataa cgcaggaact
     3841 gatgttccct gtcgccgaaa ttgctggtac acgcacacag cagcaatgcc gcccacacag
     3901 ccggtttcat acacacctcc cattaaagcc aaacattata tagccgtccc gaccgattaa
     3961 attcatattt taaaacaata ttctgtttac aaaacccaca tcgtgctata atccgcaccg
     4021 attttcagac ggcatcgtca tgccgtctga aattttttca ttccaacaac aatcagcccc
     4081 gcgattacgg ctgcctgaga aagacacaaa ccatgaaaaa agtatttatc cgcaccttcg
     4141 gctgccagat gaacgaatac gacagcgaca aaatgctcgc cgtcctcgcc gaagaacacg
     4201 gcggcatcga acaggttacc caagccgacg aagccgacat catcttgttc aacacctgct
     4261 ccgtgcgcga aaaagcgcaa gaaaaagtct tctccgattt ggggcgcgtg cgtccgctca
     4321 aagaaaaaaa ccccggcctc atcatcggcg ttgccggctg cgtcgcctcg caagaaggcg
     4381 aaaacatcat caaacgcgcg ccttatgtgg acgtggtttt cggcccgcaa acgctgcacc
     4441 gcctgccaaa aatgattgtg gacaaagaaa ccagcgggct gtcgcaagtc gatatttcct
     4501 tccccgaaat cgaaaaattc gaccacctgc cgcccgcccg cgtcgaaggc ggcgcggcat
     4561 ttgtatcgat tatggaaggc tgttccaaat actgctcctt ctgcgtcgtc ccctacacgc
     4621 gcggcgaaga attctcccgc ccgctcaacg acgtattgac agaaatcgcc aaccttgccc
     4681 agcaaggcgt gaaagaaatc aacctcttgg gacaaaacgt caacgcctat cgcggcgaaa
     4741 tggacgacgg cgaaatctgc gacttcgcca ccctgctgcg catcgtccac gaaatccccg
     4801 gcattgaacg tatgcgcttc accaccagcc acccgcgcga gtttaccgac tcgattatcg
     4861 agtgctaccg cgacctgccc aaattggttt cccacctgca cctgccgatt caaagcggtt
     4921 ccgaccgcgt attgagcgcg atgaaacgcg gctacaccgc tttggaatac aaatccatca
     4981 tccgcaaact gcgtgccatc cgtcctgatt tgtgcctgag cagcgatttc atcgtcggct
     5041 tccccggcga gaccgaacgc gagttcgagc aaaccttgaa actggtgaaa gacatcgcct
     5101 tcgacttgag cttcgtgttt atttacagtc cgcgccccgg cacgcctgcc gccaacctgc
     5161 cggacgacac gccgcacgaa gaaaaagtgc gccgcctcga agccttgaac gaagtcatcg
     5221 aagccgaaac cgcgcgcatc aaccaaacga tgatcggcac ggttcaacgc tgcctggtcg
     5281 aaggcatctc caaaaaagac cccgaccagc tccaagcccg caccgccaac aaccgcgtcg
     5341 tcaactttac cggcacgccc gacatgatta accaaatgat cgatttggaa atcaccgagg
     5401 cctacacctt ctccctgcgc ggcaaagttg tcgaagccta aacccttacg ccgaaaaaat
     5461 gccgtctgaa gcgtttcaga cggcattttg ccttgtatcg gcagacgatg gcgcggccag
     5521 gcggcttaat ttgccgcatc ccgatccgac aaccacgcgc gcacacgccg ttccaccgct
     5581 tcggcactca agcccaaatc gtctaaaagt tttttcggat cgccgtgtcc ggttacggta
     5641 tcggcaacgc ccaaaagcaa aacgggtttg cagatgccgt gtttcgccaa tacttccagc
     5701 accgcgccgc ctgcgccgcc ctgttcggcg ttttcttcaa gggtaacgat gcggtcgtgg
     5761 cttcgggcaa ggcggacaat caactcttcg tctatcggtt tgacaaagcg catatcggcg
     5821 acggtggcgt tcagttttcc ggcgaccgcc aatgcggggg cgaccatact gccgaaggca
     5881 atgaatgcgg ttttcccacc ttcgcggcgg ataatgccct tgccgatttc cacggtttcc
     5941 atgccgtctg aaaccggcgc gcccgtaccc gtgccgcgcg gatagcggac ggcggcgggc
     6001 gcatccgcct gatagcaggt cgaaagcagc aggcggcatt cgttttcatc gctcggcgcg
     6061 gcgacaatca tattcggaat gcaacgcaag aagctcaaat cgtacaaacc ggcgtgggtc
     6121 gggccgtccg cgccgacgat gcccgcgcgg tcgacggcaa acaaaacggg taggttttgc
     6181 agggcgatat cgtgcaccag ttggtcgtag gcgcgttgta aaaaggtgga ataaatcgcc
     6241 acgacgggct tcatgccttc gcacgccaaa ccgccggcaa aggtaacggc gtgctgctcg
     6301 gcaataccga catcgaaata acggtcgggg aatcgttgtt caaactccac cagtccgctg
     6361 ccctcgcgca tcgcgggcgt gatggcgatc aaacgctcgt ctgccgccgc ccggtcgcac
     6421 agccatttgc cgaacacttg ggtataggtc ggtttggcgg cgggcttggg ttctttttca
     6481 gacggcattt gcgccgcgct ttctttaggc aggttggcga cggcgtggta tttgacggga
     6541 tcgttttcgg cgagtttgta gccgttgccc tttttggtga tgacgtgcag aagctgcggg
     6601 cctttgcgtc cgcgcaggtc ttccaataca tcgaccagat tttcgacgtt gtgtccgtcc
     6661 acggggccgg tatagcggaa gccgaagttt tcaaacaaag acagtgactg tttggcgtgt
     6721 tcggcttctt cggcaagggt tttgatttta tgttcgactt tttgggcaaa ctccatcgcg
     6781 ccgggtattt tgtctaatac cttgcccgtt tgcgctttga cggtactcaa cagtccgtgc
     6841 atatcgcgca cgacgttgct ggcaaggtat ttgggcaacg caccgacgtt gggggaaatc
     6901 gacatttcgt tgtcgttgag gacgaccagc aaatccacat ccatatcgcc cgcgcagttc
     6961 aaggcttcaa acgcctgacc cgccgtcatc gcgccgtcgc cgatgatggc gacgctgcgg
     7021 cggtcgctgc ccaagagttt gtctgccgcc gccatgccca acgccgcgcc gatggaggtg
     7081 gaggaatgcc ccacgccgaa cgcgtcgtac tcggactcgc aacgtttcgg aaaacccgcc
     7141 aaaccgccat attggcgcat ggtgtgcatc tggtttttcc tgcctgtcag gattttgtgc
     7201 ggatagcttt ggtgtccgac atcccacacc agcttgtctt cgggcgtgtc gtacacatag
     7261 tgcagggcga tggtcagttc gaccgcgccc aaattgctgg cgaaatgccc gccggtctgc
     7321 ccgacagatt ccagcagaaa ggtgcgcaac tcgccggcaa ggcgcggcag ctgttttttg
     7381 tccagacggc gcaaatcttg cgggctgtca atcaggtcga gtagggggct tgggttcatg
     7441 gtgtgtcttt tttatgtgtc gtccaggtgc aacgaccaat tatatatcaa gagcgtgcgg
     7501 ctgacggctg attttgccgt atgtcattcg tcctgccgct tggcgcgcgg gtgggcttcg
     7561 tcatacaggc gggcgatgtg gtcgaaatcg agtttggtgt aaatctgcgt ggtcgaaagg
     7621 ctgctgtgcc cgagcagctc ctgcaccgcc cggatgtcgc gcgaagcctg caacaggtgt
     7681 ccggcgtagc tgtggcgcat catatgcggc gaaacgtgcc tgccgtcgcc gttttgcgcc
     7741 gcccattgcg ccaaacgttt ttggatttgg cgttggctca ggcgcgtgcc gttcctgccg
     7801 gtaaacaggg ctttgccgtc cgatgccgtc tgacgcagcg gcagatagtt tttcagggct
     7861 tccacgcttt tgccggtcag cggcacctgc cgctgcttgc gccctttgcc ggtaacgtgt
     7921 acccacgcct cgtccaaata gacatcatct gcattcaagc cgtgtatctc gctcacgcgc
     7981 aaaccgctgc cgtacatcag ctcgaacagg gcgtgatcgc gcaccgccag cgggtcgccg
     8041 ccgtccacgg gcaaatccag catccggttc agccattcct gcggcagtgc tttgggtacg
     8101 cgctcgggct gcttcggcgg tttgatgtcg gcggtcgggt cggtatgcag cagaccgcgt
     8161 ttgaccagcc aggcgcaata ctgccgccaa gacgacagct tgcgcgccag cgtccgcgca
     8221 ttcaaaccgc gctgcgacag ccgccgcaac gccgccgtaa aatcgcgccg cgacaagccc
     8281 tgcggcacgc cgccttcagc ttcagacggc atttgtgcca acagcgcaaa cagttcttcc
     8341 aaatcgcgcc ggtatgccgc cactgtgtgc tccgacttgc cctcgcgcac gatgttttcc
     8401 aaataagcgt caaaatgcgc cgcaaacccg tccaaaacca taaccgcccc acacaaatat
     8461 caaaaaaaca gtgaaaacat tatcataaac gacacaccaa aaccccgaac tgcccaaaat
     8521 ccgacccgtc cgcccgccgt tatcgttaac ataaaatcaa tgagccgttt atggtttttt
     8581 gccgcaaaaa acattataat ccgccttatt tacctattgc ccaaggagac acaaatggca
     8641 ctcgtatcca tgcgccaact gcttgatcat gctgccgaaa acagctacgg cctgcccgcg
     8701 ttcaacgtca acaacctcga acaaatgcgc gccattatgg aagccgccga ccaagtcggc
     8761 gcgcccgtca tcgtacaggc gagtgcaggt gcgcgcaaat atgcgggcgc gccgtttttg
     8821 cgccacctga ttctggcggc agtcgaagaa tttccgcaca tccccgtcgt gatgcaccaa
     8881 gaccacggcg catcgcccga cgtgtgccaa cgctccatcc aactgggctt ttcctccgtg
     8941 atgatggacg gctcgctgat ggaagacggc aaaacccctt cttcttatga atacaacgtc
     9001 aacgccaccc gtaccgtggt taatttctcc cacgcttgcg gcgtatccgt cgaaggcgaa
     9061 atcggcgtgt tgggcaacct cgaaaccggc gaagccggcg aagaagacgg tgtaggcgca
     9121 gtgggcaaac tttcccacga ccaaatgctc accagcgtcg aagatgccgt gcgtttcgtt
     9181 aaagataccg gcgttgacgc attggcaatt gccgtcggca ccagccacgg cgcgtacaaa
     9241 ttcacccgtc cgcccacagg cgacgtgttg cgtatcgacc gcatcaaaga aatccaccaa
     9301 gccctgccca atacacacat cgtgatgcac ggctccagct ccgttccgca agaatggctg
     9361 aaagtcatca acgaatacgg cggcaatatc ggcgaaacct acggcgtgcc ggttgaagaa
     9421 atcgtcgaag gcatcaaaca cggcgtgcgc aaagtcaaca tcgacaccga cttgcgcctt
     9481 gcttccaccg gcgcggtacg ccgctacctt gccgaaaacc cgtccgactt cgatccgcgc
     9541 aaatacctga gcaaaaccat tgaagcgatg aagcaaatct gcctcgaccg ctatcttgcg
     9601 ttcggttgcg aaggtcaggc aggcaaaatc aaaccggttt ccttggaaaa aatggcaagc
     9661 cgttatgcca agggcgaatt gaaccaaatc gtcaaataac aggttgcttg taaacaaaat
     9721 gccgtctgaa ccgttgttcg gacggcattt gatttttgct tctttgacct gcctcattga
     9781 tgcagtatgc aaaaaaagat accataacca aaatgtttat atattatcta ttctgcgtat
     9841 gactaggagc aaacctgtga accgaactgc cttctgctgc ctttctttga ccgccgccct
     9901 gattctgacc gcctgcagca gcggaggcgg cggtgtcgcc gccgacatcg gcgcggggct
     9961 tgccgatgca ctaaccacac cgctcgacca taaagacaaa agtttgcagt ctttgacgct
    10021 ggatcagtcc gtcaggaaaa acgagaaact gaagctggcg gcacaaggtg cggaaaaaac
    10081 ttatggaaac ggcgacagcc tcaatacggg caaattgaag aacgacaagg tcagccgctt
    10141 cgactttatc cgtcaaatcg aagtggacgg gcagctcatt accttggaga gcggagagtt
    10201 ccaaatatac aaacagaacc actccgccgt cgttgcccta cagattgaaa aaatcaacaa
    10261 ccccgacaaa atcgacagcc tgataaacca acgctccttc cttgtcagcg gtttgggtgg
    10321 agaacatacc gccttcaacc aactgcccag cggcaaagcc gagtatcacg gcaaagcatt
    10381 cagctccgac gacccgaacg gcaggctgca ctactccatt gattttacca aaaaacaggg
    10441 ttacggcaga atcgaacacc tgaaaacgcc cgagcagaat gtcgagcttg cctccgccga
    10501 actcaaagca gatgaaaaat cacacgccgt cattttgggc gacacgcgct acggcggcga
    10561 agaaaaaggc acttaccacc tcgccctttt cggcgaccgc gcccaagaaa tcgccggctc
    10621 ggcaaccgtg aagataaggg aaaaggttca cgaaatcggc atcgccggca aacagtagca
    10681 tttgaaaaat gccgtccgaa cacgatagtt taccgttcgg acggcatttt tgctccatac
    10741 tgccctagga cacgacggca cggcaggcaa gccggtttca ctatataatg ccgtctgaac
    10801 caacgagaac cacaccatgc aagccgattt caaccgcccc gtcctcgccg tcgataccgg
    10861 aacttcctat ttgtcgctcg cgctgcgcgc cgacggcgaa accctcctgt tccatcagga
    10921 ggccggcagc cgccagtccg aactgatcct gccggaaatc cgcaccctat tccgaaatgc
    10981 gggcattacc gccgccgatt tgggggcgat tgtttatgcg aaaggtcccg gcgcgtttac
    11041 cggactgcgt atcggcatcg gcgtggcgca aggtttggca acgccattca atacgccttt
    11101 aatcggcatc cccacgctcg atgccgccgc ctcgctgccg ccgccgcaaa gctgcatact
    11161 cgccgctgcg gacgcgcgta tgggcgaagt gttttatgcg tggttcgaca cgctgaaccg
    11221 ccgccgtttg agcgattatc aggtcgggcg ggcggcagac atccggctgc cggagggatg
    11281 tgccttttca gacggcatag gcagcgcgtt cgcgttggaa gaaacgccgc cgttttcagg
    11341 caaacccgat atgccgaccg ccgccgactt cctcaatctg gcggcggaag gcggttttcc
    11401 cgccgtcgct gcggcacact ccgaactgct ctacgtccgc aacaaaatcg ccctaaccgc
    11461 caaagaacag gcggaacgga aagcccgaac atgaacatcc gccgcgcctc accgtccgac
    11521 tgcgccgcac tcgccgcgct cgatgccgtc tgcaacccgt ccgcgtggac gcaacgccaa
    11581 tttgagtccg cattggtttc gccgtccgaa caggttttcc tcgcggaaaa agacggcggg
    11641 attgccgcct ttatcgtttg gcagaacctg cccgacgaat ccgaactgca cctgattgcc
    11701 accgcgcccg aatgccgccg ccgtggcgtt gcgtccgccc tgctcgaata ttggttcgca
    11761 catctgcccg aagacacgca acgcctgctg ctcgaagtcc gtgcaggcaa caccgccgca
    11821 caggcactgt acgcggcgca cggctttacc gatgcgggca ggcggaaaaa ctattaccgt
    11881 acagccgacg gtaaaaccga agatgctgtc ttaatggaga aaatatgtta agcgcgcgct
    11941 acctccacct gcacgaagct ttgggtttag gcccgatgtg gctgaaacag gccgccgccg
    12001 tcctgccgcc caaaaacaca cccgcaaccc cggcacaggc acgtcctcaa accgtccgcg
    12061 ccgccccgat ccgcccttcc caaccccata acggtcaggc gcggctcgaa acgatgaaag
    12121 cgttggaaac cgccgccgta catacgcgca aacccgcgcc tgaaaccgaa acgcccccgc
    12181 ccggcctttc agacggcatc gcccccgttc ccgccgcttc gggcataacc aaacttgccg
    12241 tcgtcagcct ttgcccaccg attgaggatg cggtttacgg gcaactgttc tacggcaaag
    12301 cgggtgttct gcttgacaac atactcaaag ccgtaggact ggatgccgcc tatgtccaca
    12361 aaacctgttg ggtgaaaacc gccgccgtcg gcaacccgat gccgtctgaa gcggcaatcg
    12421 aaaccgcgac ggtttccgtc atgcaggaac tcgacggctg ccgcgccccc gccgtcctat
    12481 tcctcggaca ggcttttgtc cagcctgaac ggcaaacgat gattgaaact ttgtgcggca
    12541 gccgtccctt cttcatcatc gaccatcccg cccggctgtt gcgccaaccc gaacttaaag
    12601 cccgcgcctg gcaggtgttg aaacagttga aacgcgcctt gcggcaaggc ggcggcagtt
    12661 gaagcgcacc gcacggggcg gtagaatcgc aactgcgtcc caatatctga cagaaagcac
    12721 aaaatgaccg atttccgcca agatttcctc aaattctccc tcgcccaaaa tgttttgaaa
    12781 ttcggcgaat ttaccaccaa agccggacgg cggtcgccct atttcttcaa tgccggcctc
    12841 tttaacgacg gcttgtccac gctgcaactg gcaaaatttt acgcacaatc catcattgaa
    12901 agcggcatcc gattcgatat gctgttcggc cccgcctaca aaggcattat tttggcggcg
    12961 gcaaccgcga tgatgctggc ggaaaaaggc gtgaacgtcc cgtttgccta caaccgcaaa
    13021 gaagccaaag accacggcga aggcggcgtg ttggtcggtg cgccgcttaa aggccgcgtg
    13081 ctgattatcg acgacgtgat ttccgccggc acatccgtac gcgaatcgat caagctgatt
    13141 gaagcggagg gcgcaacccc cgccggtgtc gccatcgcgc tcgatcgcat ggaaaaaggc
    13201 acgggtgagt tgagcgcggt tcaggaagtg gaaaaacaat acggtctgcc cgtcgccccc
    13261 atcgccagcc tgaacgattt gtttattctg ttgcaaaaca accccgaatt cggacagttc
    13321 ctcgaacccg tccgagccta ccgtcggcag tacggcgtag aataaaaaca aagcatatgc
    13381 cgtccgaacc gccttacgcc tcagacggca tcaaacctga cacacacgag gaaataccat
    13441 gcccgcctgt ttctgccccc actgcaaaac ccgtctctgg gtcaaagaaa cccaactcaa
    13501 tgtcgcccaa ggcttcgtcg tctgccaaaa atgcgaagga ctgtttaaag ccaaagacca
    13561 tctggcaagc acgaaagaac ccatattcaa cgatttgccc gaggctgttt cggatgtcaa
    13621 actcgttcac cgtatcggca cgcgcgccat cggcaagaaa cagatttccc gtgacgaaat
    13681 cgccggcatc ctcaacggcg gcacaaccca gcccgatatt ccgcccgcaa ccgccgccac
    13741 ccctgctgcc gcaccgcagg ttaccgtacc gcccgccgcg cccgcccgtc aggatgggtt
    13801 caactggacg attgcaaccc tgtttgccct tatcgtcctc attatgcagc tttcctacct
    13861 cgtcatccta tgatcgtgcc cgacctcttt gtcgcccact tccgcgaagc cgccccctac
    13921 atccgccaaa tgcgcggcaa aacgctggtc gccggcatag acgaccgcct gctcgaaggt
    13981 gataccttaa acaagctcgc cgccgacatc gggcttttgt cgcaactggg catcaggctc
    14041 gtcctcatcc acggcgcgcg ccacttcctc gaccgccacg ccgccgcgca aggccgcacg
    14101 ccgcattatt gccggggctt gcgcgttacc gacgaaacct cgctcgaaca ggcgcagcag
    14161 tttgccggca ccgtccgcag ccgttttgaa gccgcattgt gcggcagcgt ttccgggttc
    14221 gcgcgcgcgc cttccgtccc gctcgtatcg ggcaacttcc tgaccgcccg tccgataggt
    14281 gtgattgacg gaaccgatat ggaatacgcg ggcgttatcc gcaaaaccga caccgccgcc
    14341 ctccgtttcc aactcgacgc gggcaatatc gtctggctgc cgccgctcgg acattcctac
    14401 agcggcaaga ccttccatct cgatatgctt caaaccgccg cctccgtcgc cgtctcgctt
    14461 caggccgaaa aactcgttta cctgaccctt tcagacggca tttcccgccc cgacggcacg
    14521 ctcgccgtaa ccctctcggc acaggaagcg caatcgctgg cggaacacgc cggcggcgaa
    14581 acgcgacggc tgatttcgtc cgccgttgcc gcgctcgaag gcggcgtgca tcgcgtccaa
    14641 atcctcaacg gtgccgccga cggcagcctg ctgcaagaac tcttcactcg caacggcatc
    14701 ggcacgtcca ttgccaaaga agccttcgtc tccatccggc aggcgcacag cggcgacatc
    14761 ccgcacattg ccgccctcat ccgcccgctg gaagaacaag gcatcctgct gcaccgcagc
    14821 cgcgaatacc tcgaaaacca catttccgaa ttttccatcc tcgaacacga cggcaacctg
    14881 tacggttgcg ccaccctaaa aacctttacc gaagccgatt gcggcgaaat cgcctgcctt
    14941 gccgtctcgc cgcaggcaca ggacggcggc tacggcgaac gcctgcttgc ccacattatc
    15001 gataaggcgc gcggcatagg cataagcagg ctgttcgcac tgtccacaaa taccggcgaa
    15061 tggtttgccg aacgcggctt tcagacggca tcggaagacg agttgcccga aacgcggcgc
    15121 aaagactacc gcagcaacgg acggaactcg catattctgg tgcgtcgcct gcaccgctga
    15181 ccgcaacgga aagccgccgc agaaatgccg tctgaacccc gtttcagacg gcatttcccc
    15241 gattatatag tggattaaca aaaaccagta cggcgttgcc tcgccttgcc gtactatttg
    15301 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata aagacctgcc
    15361 caaccctcaa ggaccccgat gaaatcctac cccgacccct accgccattt tgaaaacctc
    15421 gattccgccg aaacgcaaaa cttcgctgct gaagcgaatg ccgaaacgcg cgcgcgtttt
    15481 ttaaacaacg acaaggcgcg cgcgctttca gacggcattt tggcgcagat gcaggacacg
    15541 cggcagattc cgttttgtca ggaacaccgc gcgcggatgt accatttcca tcaggacgcg
    15601 gagtatccga agggcgtgta ccgcgtgtgt gcggcggcga cgtatcgttc cggctatccc
    15661 gagtggaaaa tcctgttttc agtggcggat ttcgacgagc tgctcggcga cgatgtgtat
    15721 ttgggcggcg tgtcgcactt ggtggaaaag cccgaccgtg cgttgttaac actgagcaaa
    15781 tcgggcggcg atacggcgta cacgctggaa gtggatttgg aagcagggga gttggtagaa
    15841 ggcggttttc actttccggc aggcaaaaac catgtgtcgt ggcgcgatga aaacagcgtg
    15901 tgggtgtgtc cggcctggga cgagcggcag ttgaccgaat cgggctatcc gcgcgaggtg
    15961 tggctggtgg aacgcggcaa aagtttcgag gaaagcctgc cggtgtacca aatcggcgaa
    16021 gacgggatga tggtgaacgc gtggcgttac ctcgatccgc agggttcgcc gattgatttg
    16081 attgaagcgt ctgacggttt ttacaccaaa acctatttgc aggtgtcggc cgaaggcgag
    16141 gcgaaaccgt taaacctgcc tgctgattgc gatgtggtcg gctatctggc ggggcatctt
    16201 ttgctgacgc tgcgcaagga ctggcaccgc gcgaaccaaa gctatccgag cggcgcgctg
    16261 gtagcggtga aactgaatcg cggcgaaatc ggggcggcgc agcttttgtt tgcgcctgat
    16321 gaaacgcagg cattggaaag cgtggaaacg accaagcgtt ttgtcgtggc gagtctgctg
    16381 gaaaacgtac aaggccgtct gaaagcgtgg cgttttaccg acggcaaatg gcaggaagcc
    16441 gaactgccgc gcctgccctc gggcgcgttg gaaatgaccg accaaccgtg gggtggcgac
    16501 gtggtttacc ttgccgccag cgatttcacc acgccgctga cgctgtttgc gctggatttg
    16561 aacgtgatgg aactgaccgt catgcgccgt cagccgcagc agttcgattc agacggcatc
    16621 aacgtgcagc agttttggac gacttcggcc gacggcgagc gtattcctta ttttcacgtc
    16681 ggcaaaaacg ccgcccccga cacgccgact ttggtttatg catacggcgg ttttggcatt
    16741 cccgagttgc cgcattattt gggcagcgtc ggcaaatatt ggctggaaga gggcaatgcc
    16801 tttgtgttgg caaacatccg cggcggcggc gagttcggcc cgcgctggca tcaggcggcg
    16861 cagggaatca gcaaacacaa aagcgtcgac gatttgcttg ccgtcgtgcg cgatttgtcc
    16921 gaacgcggta tcagttcgcc ccaacacatc ggcttgcagg gcggcagcaa cggcggcctg
    16981 attaccgccg ccgccttcgt gcgcgaaccg caaagcatcg gcgcgctggt gtgcgaagtg
    17041 ccgctgaccg atatgatccg ctatccgctg ctctccgccg gcgcaagctg gacggacgaa
    17101 tacggcaacc cgcagaaata cgaagtctgc aaatgccggt tgggcgaatt gtcgccgtat
    17161 cacaatcttt cagacggcat cgattatccg ccagcgctca ttaccaccag cctcagcgac
    17221 gaccgcgtcc atcctgccca cgcgctcaag ttctacgcca aactgcgcga aacctccgcg
    17281 caatcttggc tctactcacc tgacggcggc ggccataccg gcaacggcac ccaacgcgaa
    17341 tccgccgacg aactcgcctg cgtgttgctg tttttgaaag agtttttggg ctaagggcgg
    17401 ggagcggcac tgccgccgcg aatgaaaaaa ggtcgtctga aactgctttt tcagacgacc
    17461 ttttttaatg gttgtttcaa atcaaaatat ctatgccgcc ggccccatca gcacttcttc
    17521 acatccgaag gcaaaaatcc gtaatgccgt ctgaacgctt cgttgaaccg tcccgcgtgg
    17581 cggtagccgc aaaagtgcat ggcggcttgg acggtgctgc cggattcgat gagggcgagc
    17641 gcgtgttcca gccgcaggcg gcgcaggcat ccggcgacgg tttcgccggt ttgcgctttg
    17701 aaatagcgtt tcaggtagca ttcgttcagt ccgacgcggc gggcgatttc ggcgatggtc
    17761 agcggacggg cgaattcgtg ttgcaggatg tcggcggctt cgtctatgcg ccgacggcgg
    17821 taaccgttgt cgtggcggcg gaaggtgaag cgcaataatc gggcggagag ttccagcgcg
    17881 gcggcttcgt cggcaagcag gccgaagccg tccgattcga acgggcgttg cagcagtggg
    17941 caggccgccg ccgtcagtgc cgccgcgttt tgcgccagcc gttgcagggc gaatcggcct
    18001 atcgtttgcg gcgaaaacag gcgttcgtcc agcaagcctt cgtcgtgcca gcggcgcagt
    18061 ttttccagcg aaaaatccaa atgcagcgcg cacatgccgc tgttgtcggg cagcagggtt
    18121 tcggatacgt ccgccaactc gccgcgtacc agccagattt cgccggcaga cgggcggtat
    18181 tccctgccgc ccatttgtaa ccggttctgc cccgacacca tgacgaacaa ggcgcagttg
    18241 tggctgaaat tgtggatttc ggtgggaaac gcgcccgttc cgccgccgcg catccgcgac
    18301 aaggtgatgc ccgaatcgaa gcggttgatg cacatttcca gatgcaaacc gggctgtttt
    18361 gcctgcgcaa tgagcgcgct gtcggaacag ccgtccaacg cccagccgga tttatcggag
    18421 cggacatagg tttggtactg gcggtagatg gcggcggtgt tcatgattgg ataggaacga
    18481 gttgtctaac aaatgaatta aataggaatt attaccaata atcaagcgca gggattggtt
    18541 gaaacggaaa aggtcgtctg aaagggtgtt tcagacgacc ttttccgtat cgggaatttg
    18601 ttttgccgta tcgggaattt tgcgttttgc ggcgtggttt ctgcaggttg tttgcttaat
    18661 aataaacatt cttattcgta tgcaaaggaa ccgcacaccg tgaaaccgcg tttttattgg
    18721 gcagcctgcg ccgtcctgct gaccgcctgt tcgcccgaac ctgccgccga aaaaactgta
    18781 tccgccgcat ccgcatctgc cgccacactg accgtgccga ccgcgcgggg cgatgccgtt
    18841 gtgccgaaga atcccgaacg cgtcgccgtg tacgactggg cggcgttgga tacgctgacc
    18901 gaattgggcg tgaatgtggg cgcaaccacc gcgccggtgc gcgtggatta tttgcagcct
    18961 gcatttgaca aggcggcaac ggtggggacg ctgttcgagc ccgattacga agccctgcac
    19021 cgctacaatc ctcagcttgt cattaccggc gggccgggcg cggaagcata tgaacagttg
    19081 gcgaaaaacg cgaccaccat agatctgacg gtggacaacg gcaatatccg caccagcggc
    19141 gaaaagcaga tggagacctt ggcgcggatt ttcggcaagg aagcgcgcgc ggcggaattg
    19201 aaggcgcaga ttgacgcgct gttcgcccaa acgcgcgaag ccgccaaagg caaaggacgc
    19261 gggctggtgc tgtcggttac gggtaacaag gtgtccgcct tcggcacgca gtcgcggttg
    19321 gcaagttgga tacacggcga catcggccta ccgcctgtag acgaatcttt acgcaacgag
    19381 gggcacgggc agcctgtttc cttcgaatac atcaaagaga aaaaccccga ttggattttc
    19441 atcatcgacc gtaccgccgc catcgggcag gaagggccgg cggctgtcga agtattggat
    19501 aacgcgctgg tacgcggcac gaacgcttgg aagcgcaagc aaatcatcgt catgcctgcc
    19561 gcgaactaca ttgtcgcggg cggctcgcgg cagttgattc aggcggcgga gcagttgaag
    19621 gaggcgtttg aaaaggcaga acccgttgcg gcggggaaag agtagggagt cgtctgaaaa
    19681 cggagtttcc gaaggaagcg gggggtttct gcgaagctaa agtgcggttt caacgaattg
    19741 aaaagcagcc tgtatgttga aaataccgc
//
LOCUS       DAWHXS010000034        19460 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWHXS010000034 DAWHXS010000000
VERSION     DAWHXS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.034"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..203)
                     /locus_tag="WIE22_000902"
     CDS             complement(<1..203)
                     /locus_tag="WIE22_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4127009.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMLKAVLLG"
     gene            complement(266..1084)
                     /gene="hslO"
                     /locus_tag="WIE22_000903"
                     /pseudo
     CDS             complement(266..1084)
                     /gene="hslO"
                     /locus_tag="WIE22_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            1329..2084
                     /locus_tag="WIE22_000904"
     CDS             1329..2084
                     /locus_tag="WIE22_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ4127010.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            2103..2321
                     /locus_tag="WIE22_000905"
     CDS             2103..2321
                     /locus_tag="WIE22_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127011.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            2466..2810
                     /locus_tag="WIE22_000906"
     CDS             2466..2810
                     /locus_tag="WIE22_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ4127012.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            2810..3502
                     /locus_tag="WIE22_000907"
     CDS             2810..3502
                     /locus_tag="WIE22_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ4127013.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            3569..4789
                     /gene="argJ"
                     /locus_tag="WIE22_000908"
     CDS             3569..4789
                     /gene="argJ"
                     /locus_tag="WIE22_000908"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ4127014.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            4849..6285
                     /locus_tag="WIE22_000909"
     CDS             4849..6285
                     /locus_tag="WIE22_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ4127015.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLTAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTAKRECRLKIKTPPIKRRQPP"
     gene            6440..7831
                     /gene="hrpA"
                     /locus_tag="WIE22_000910"
     CDS             6440..7831
                     /gene="hrpA"
                     /locus_tag="WIE22_000910"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ4127016.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     RRLAALPKPEFDNTLPVHEKLEEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            7932..9059
                     /locus_tag="WIE22_000911"
     CDS             7932..9059
                     /locus_tag="WIE22_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127017.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            9237..10813
                     /gene="lpt3"
                     /locus_tag="WIE22_000912"
                     /pseudo
     CDS             9237..10813
                     /gene="lpt3"
                     /locus_tag="WIE22_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
     gene            10915..13995
                     /locus_tag="WIE22_000913"
     CDS             10915..13995
                     /locus_tag="WIE22_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ4127018.1"
                     /translation="MDARSNPVNVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEIRQLTSSESQGDQDLAAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     NFYTADAVSDDLHPTNPQQTAPSPVGEGWGEGKTVAAQTNFSATAANPLPNPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGWGEGKTVAAQTNFSATAANPLPQEGEQSAA
                     ASALSDDPQPQKQPASQKGRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDLMQHA
                     AAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPG
                     MLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAAIIPQLAHFIAKSAGDMR
                     IFEQIDQDAWAVQELPEHCYLNLRIIDDGGQELAGGRKLHELQQQLGQAAATTFRDNT
                     QEFERDNVTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTIEAAEQAH
                     RQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGED
                     ELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRL
                     QTLLAPSFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEK
                     TDSLVKQGLPISDGLAGFKWMIEELRVSLFAQELKTPYPVSVKRLMKEWERLNK"
     gene            14054..15661
                     /locus_tag="WIE22_000914"
     CDS             14054..15661
                     /locus_tag="WIE22_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ4127019.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNAIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPLKFKPNILF
                     HKGDKKSDVNERLIHSLTQSISDIRKQLIDTQKQVEKKYEKYQQYLQSPFVAQADIDV
                     AISGILDQIDKLNLRIKDCERLESLCR"
     gene            15652..17112
                     /locus_tag="WIE22_000915"
     CDS             15652..17112
                     /locus_tag="WIE22_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEZ4127020.1"
                     /translation="MSLNRKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQAKLALLQKNPFILHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPNKQNLPLKLLTGKWQQQLDYAKSQWYLKQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYTKSPQIDVNSREEIIGLRLGKDLEYVLPSELALMADEETSTLFDLK
                     FLESKLMCFELQGMTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQEIYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQELVQFKKDIFNKQVVNS"
     gene            complement(17466..17939)
                     /locus_tag="WIE22_000916"
                     /pseudo
     CDS             complement(17466..17939)
                     /locus_tag="WIE22_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            18171..18383
                     /locus_tag="WIE22_000917"
     CDS             18171..18383
                     /locus_tag="WIE22_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127021.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            18680..18835
                     /locus_tag="WIE22_000918"
     CDS             18680..18835
                     /locus_tag="WIE22_000918"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ4127022.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(18925..19374)
                     /locus_tag="WIE22_000919"
     CDS             complement(18925..19374)
                     /locus_tag="WIE22_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ4127023.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         5161 a         5370 c         4533 g         4396 t
ORIGIN      
        1 tgtccgagca ggacggcttt gagcatggac agcaggggat aggcaggacg gccgcggtgg
       61 tctcggaggt aacgggttct ttgacggttc aggtattgtt cgatcggttg ccaatcaatc
      121 acttgatcta gcttcaatag tgggaagcgg tcgatgtgtt tggcaatcat ggcttgggcg
      181 gtttgctgga agaaggtgct catgagaaat cccctaaatg tcttggtggg aatttagggg
      241 attttgggga attttgcaaa ggtctcttgc tgtggcagaa atcgcaatcg acttcgatgc
      301 tgccttgttc caccaccacg ccgccgactt cttccccgcc cagcatcagc agcatatcgc
      361 tgactttgcc gcgcgaacag gtgcatgaaa attcaaacgt ttccggctcg aacacgcgcg
      421 gcggcgtttc gtggaacagg cggtacaaaa catgttgcgc atccaattct gccagctcgt
      481 ccgccgtcag tgtacgcgcc agcgtactga cgtgttccca tgcctcttca tccaacaccg
      541 cttcaggcag acgctgcacc agcagaccgc ccgccgcttc gtcgcttgca gacaggacga
      601 tgtgcgtatc gatttgttcg gaacgcttca tatagttcgc caacatttgc gcgataccgc
      661 cgccttccaa aggcactacg ccctgccagg gttcgccgtc tttgggctgc agcgtcagca
      721 cgaatacgcc gccctcgccc aaaaggtcgc cgaggctttc gtcatcggct atttctgcgg
      781 tttcgtccca acgcgcggtt gcacggacgg tacggtcgga agccgcttcc gcaaccagca
      841 ttttcagccg cccccgcccc tgaacctgca caatcagcgt gccttcgttt ttgaggttgc
      901 ccgacagcaa cacacccgcc gccaacaact cacccaaagc gcggcggatg gcggcgggat
      961 agtttttctg ttttacaatg tgctgccaca cgttttccag acggacgtgc agcccgcgca
     1021 cgggcatatc gtcgaagata aagcgggtac gcacatcggc gcggttgatg gcggtttgat
     1081 tcatgatttt ctctgactga ttgttcggat ggcggctata tggttgcggt cggcgcgaaa
     1141 acaagacgga cggcggatgc gcttcccaaa ttatcaataa attatataaa aatcaacata
     1201 ttaactcaat ctaacaagcc gttttttgcc aaacagccgt ttttttatat acaatcaaca
     1261 agatattttc gactgataca gcataacatc gcacggcggc acgatgcctc ctgcgcggaa
     1321 acaccgatat ggattctttt ttcaaaccgg cagtttgggc ggttttgtgg ctgatgtttg
     1381 ccgtccgccc cgcccttgcc gacgagttga ccaacctgct cagcagccgc gagcagattc
     1441 tcagacagtt tgccgaagac gaacagcccg ttttacccat caaccgagcc cccgcccggc
     1501 gggcgggcaa tgccgacgaa ctcatcggca gcgcgatggg gcttaacgaa cagcccgttt
     1561 tacccgtcaa ccgagtcccc gcccggcggg cgggcaatgc cgacgaactc atcggcaacg
     1621 cgatggggct taacgaacag cccgttttac ccgtcaaccg agcccccgcc cggcgggcgg
     1681 gcaatgccga cgaactcatc ggcaacgcga tgggactttt gggtattgcc taccgctacg
     1741 gcggcacatc ggtttctacc ggttttgact gcagcggctt catgcagcac atcttcaaac
     1801 gcgccatggg catcaacctg ccgcgcacgt cggcagaaca ggcacggatg ggtacgccgg
     1861 ttgcccgaag cgaattgcag cccggagata tggtgttttt ccgcacgctc ggcggcagcc
     1921 gcatttccca tgtcggactt tatatcggca acaaccgctt catccacgcg ccgcgcacgg
     1981 ggaaaaatat cgaaatcacc agcctgagcc acaaatattg gagcggcaaa tacgcgttcg
     2041 cccgccgggt caagaaaaac gacccgtccc gctttctgaa ctgatttccc aaggaatacg
     2101 caatgagtat gcccgaaatg cccaaatggt acgacgatga cggacagatc gtgtcctgta
     2161 ccgaaaaggt caaagtgatg tccgaaaata tggccgagct gtatcagacg gcacaagacg
     2221 cgtttgaaga cgcgctgctg atgggttgcg gcgaacgtca attgcgcgat tacctgctcg
     2281 cgctgattga aggtttggaa aatccctacc gcaaagtctg aacacgcccc ggttgctgcg
     2341 gcacggttta tccgtgccgt ttttgcgttt gtgcgcggct tcggcttttc agacggcata
     2401 tttgacgtta tgattaaaca gttaacaaga tttatcacaa cgccgtcaaa agacagacac
     2461 acaacatgaa catcatccgc gcgctcctca tcatcctcgg ctgcctcgcc gccggcgaaa
     2521 ccgccgtttt cctagcaggc atcaaactgc ccggcagcat cgtcggcatg ggcgtgctgt
     2581 ttgcgctttt gcaggcgggt tgggtcaaaa cgtcttggct acaacagctt accgacgcgc
     2641 tgatggcgaa tctgacgctg tttctcgtgc cgccctgcgt ggcggtcatc agctatttgg
     2701 atttgattgc cgacgattgg ttttcgatac tggtttccgc ctccgccagc actttgtgcg
     2761 tactgctggt tacaggcaag gttcaccgct ggatacggag cattatctga tgaacgaaat
     2821 cctcaggcag cccagcgttc tgcttttcct cacgcttgcc gtgtacgcgc ttgcgattat
     2881 cgtgcgcacg cgcacgggca atatcttctg caaccccgta ctcgtcagca ctatcgtgct
     2941 gattgcctac ctgaaaatcc tcgacatcga ttatgcggtg taccacaacg ccgcgcaatt
     3001 cattgatttt tggctgaaac ccgccgtcgt cgtgcttgcc gtgccgctct accaaaaccg
     3061 ccgtaaaatc ttcaaccagt ggctgcccgt catcgtttca cagcttgcgg gcagcgttac
     3121 gggcattgtt acggggatgt attttgccaa atggctgggc gcggaacgcg aagtcgtcct
     3181 ctcgctcgcg tccaaatctg ttaccaaccc catcgccatt gaaatcaccc gctccatcgg
     3241 cggcattccc gccattaccg ccgccaccgt catcattgcc ggtctggtcg gacagattgc
     3301 cggttacaaa atgctgaaga acacagtcgt catgccctcg tccgtgggta tgtcgctcgg
     3361 cacggcttcg cacgcgatgg ggattgccgc ctcgctcgaa cgcagccgcc gtatggcggc
     3421 atacgcggga ctggggctga cgttcaacgg cgtactgacc gcgctgattg cgccgctgct
     3481 catccccgtt ttgggatttt gaacccgttt cagacggcat ttcagcccat gctgtctgaa
     3541 cgccgacaca ctcgcaagga gaaccgttat ggctgtcaac ctgaccgaaa aaaccgccga
     3601 acaactgccc gacatcgacg gcattgccct ctacaccgcc caagcaggcg tgaagaagcc
     3661 cgggcatacc gacctgacac tgattgccgt agccgccggc agcaccgtcg gtgcagtctt
     3721 cacgaccaac cgtttctgtg ccgcgcccgt ccacatcgcc aaatcgcacc ttttcgacga
     3781 agacggcgtg cgcgccctca tcatcaacac gggcaacgcc aacgcgggta cgggcgcaca
     3841 gggcagaatc gatgctttgg cagtgtgtgc cgccgccgcc cggcaaatcg gctgcaaacc
     3901 gaaccaggtg ctgcccttct ccaccggcgt gattctcgaa ccgctgcccg cagacaaaat
     3961 catcgccgcg ctgcccaaaa tgcagcctgc cttctggaac gaagcggcac gcgccatcat
     4021 gaccaccgac accgtgccca aagccgcctc gcgcgaaggc aaggtcggcg acaaacacac
     4081 cgtccgcgcc acgggcatcg ccaaaggctc gggcatgatt catcccaata tggcgaccat
     4141 gctcagtttc atcgccaccg atgccaaagt ttcccaaccc gtcctccaac tgatgacgca
     4201 ggaaatcgcc gacgaaacct tcaacaccat caccgttgac ggcgacacca gcaccaacga
     4261 cagcttcgtc atcatcgcca ccggcaaaaa cagccaaagc gaaatcgaca acatcgccga
     4321 cccgcgttac gcccaactca aagaattgtt gtgcagcctc gcgctcgaac tcgcccaagc
     4381 catcgtccgc gacggcgaag gtgcgaccaa gttcatcacc gtccgcgtcg aaaacgccaa
     4441 aacccgcgac gaagcccgcc aagccgccta cgccgtggca cgttcgccgc tggtcaaaac
     4501 cgcctttttc gcctccgacc ccaacctcgg cagactgctc gccgccatcg gttatgccgg
     4561 cgttgccgac ctcgataccg acctcgtgga aatgtatctc gacgatattt tggttgccga
     4621 acacggcgga cgcgccgcaa gctacaccga agcacaaggg caggcggtga tgtcgaaggc
     4681 cgaaatcacc gtccgcatca agctgcatcg cggacaagcc gccgccaccg tctatacctg
     4741 cgacctgtcg cacggatacg tttccatcaa cgccgactac cgttcctgac ccgacacggc
     4801 ttcagacggc atacataaaa tgccgtctga accgccggac aacataccat gacctccaca
     4861 ttcccccgcc gcctcgcccg caaaatccgc caaacccgcc gcctgtcgcg caaaagcatc
     4921 gcctttctgt tccttttggc aggttcggca ctcgtcgccc tgaccgcgct gttttttgcc
     4981 catcttgccg attttgcgct ggaactgaac gccaaactgg ttcaacaata cccgtggttc
     5041 gcgtgggtcg cgcttccttt gggtttaccg cttattgcgt ggctcacacg caaattcgcc
     5101 cccttcaccg ccggcagcgg catcccgcag gtcatcgcct cactgtcgct gccctacggc
     5161 gcacagaaaa cgcggctgat ccgcctcggg cagacgctgc tgaagattcc gctgaccttt
     5221 ttgggtatgc tgttcggcgc gtccatcgga cgcgaaggtc cgtccgtgca ggtcggcgcg
     5281 gcagtgatgg gcgcgtgggg cgcgtggtgc aagaaacacg gcttggcatt caaagggatg
     5341 caggaaaacg atttgatggc ggcgggcgcg gcgggcggtt tggcagccgc gttcaacgcg
     5401 ccgctggcgg gcgtgatttt cgccattgag gaactcgggc gcggcatcat gttgcgctgg
     5461 gaacggcaga ttctgctcgg cgtactcgcc gccggtttca tacaggtcgc cattcagggc
     5521 aacaacccgt atttttccgg cttcaacggc ggcgtattgg aacatatctt tctgtgggtc
     5581 gcactgtccg gcctggtttg cggcgcggcg ggcgggctgt tcggacgttt gctctatcgc
     5641 ggtgcggcgg cgtttgcacc gcgcaagata cgcggcttca tccgcaaccg tccgctgctg
     5701 ctggcggcac tgatggggct gctgctcgcc ctgctcggca cgttctacca aggcaaaacc
     5761 tacggcaccg gctaccacga agccgcccaa gccctgcacg gcatctacga agcccccttc
     5821 ggactcaccg ccgccaaatg gctcgccacc gtattcagct attgggcagg cattccgggc
     5881 ggcattttca ctccctcgct gaccataggc gcggttttgg gcgagcatat cgccgccatc
     5941 gccgacatat cgcagggtgc aaacatcatc gtcctcatct gcatggcggc atttctggcg
     6001 ggcgcgacac aatccccgat tacttccgcc gtcgtcgtca tggaaatgac gggcggacaa
     6061 agcctgctgt tttggatgct gattgcctgc attttcgcct cgcaggtttc gcgccagttt
     6121 tcgccgcgtc cgttctacca cgcatcggga atgcgcttcc gccagcgcgt gcttcaagaa
     6181 accgccgccc aaaccggcaa tgcgcccgca agaccgcaaa cagcaaaacg ggaatgccgt
     6241 ctgaaaatta aaacgccccc gatcaaacgc cggcagccgc cttgattcga ataccgttcc
     6301 gccgccgctt gaaatttcag caacaatgcc gtctgaacga cagaatgcgg ttttcagacg
     6361 gcatttcccc atcccgatat tgcctaaaca aaaccgaagc gtttgctata attgcacttt
     6421 ttaccgcaca cgcacgccca tgtttcccga tttctcccaa accctctcca aagaccgcca
     6481 cttcctgcaa tccgccttca aaaatcccaa caaatacggc ggtttgtcca aagtcgaaga
     6541 aaaataccga aaatcgcacg aaatcttttt gaggcgtttg gccgcattgc ccaaacccga
     6601 attcgacaac accctgcccg ttcacgaaaa actcgaagaa atcaaaaaaa ccgttgccga
     6661 acatcaggta acgattattt gcggcgaaac cggttcgggc aagaccactc agttgcccaa
     6721 gatttgtttg gaactcgggc gtggggcggc ggggctgatc gggcataccc agccgcgccg
     6781 tttggccgcg cgctccgtgg cagagcggat tgccgaagag ctgaaatccg aaatcggcag
     6841 cgcggtcggc tataaagtac gcttcaccga ccacacctcg cgcgatgcct gcgtcaagct
     6901 gatgaccgac ggcatcctgc tggcggaaac gcagaccgac cgttatctcg ccgcctacga
     6961 cacgattatc atcgacgaag cgcacgagcg cagcctgaac atcgacttcc ttttgggcta
     7021 tttgaaacaa ctcctgccgc gccgccccga tttgaaagtc atcatcacct cggcaacgat
     7081 agacgcagaa cgcttctccc gacacttcaa cggcgcgccc gttttagaag tgagcggacg
     7141 gacgtatccc gtcgaaatcc tctaccgacc gctgaccggc aaagacgaag acgacgcaga
     7201 agtggagttg accgacgcga ttgtcgatgc ggcggacgaa ttagcgcgac acggcgaagg
     7261 cgatattttg gtattcctgc cgggcgagcg cgaaatccgc gaagccgccg aagccctgcg
     7321 caaatccacg ctgcgccgca acgacgaaat cctgcccctg ttcgcacgcc tgtcgcacgc
     7381 cgagcagcac aaaatcttcc acccctcagg cgcgaaacgc cgcatcgtat tggcaaccaa
     7441 cgtcgccgaa acctcgctta ccgtgccggg catcaaatac gtcatcgaca ccggcctcgc
     7501 gcgtgttaaa cgctattccg cacgggcgaa agtggagcag cttcatatcg aaaaaatctc
     7561 ccaagccgcc gcccgccaac gatccggccg ctgcggacgc gtctccgcag gcgtgtgtat
     7621 ccgactgttt tcagaagaag atttcaacag tcgccccgaa tttaccgacc ccgaaatcgt
     7681 ccgcagcaac ctcgccgccg tcatcctgcg catggcatcg ctgaaactcg gcgacgtagc
     7741 ggcattccca tttttagaaa tgcccgattc gcggtatatt aatgatgggt ttcaggtgtt
     7801 gctggaatta ggggcggtgg aggccgcctg aaaacaagta ggatatacac ttctacctga
     7861 caaagaccaa caaacgataa aactacggca catctagatg cccaacctac aacattgcac
     7921 acggtaaaaa catgaaacac ttactcatcg attttgaaaa cgtccagccg caaaacttag
     7981 acaaattact aaccgaaaat acccatattt ggctatttat aggtgtatta cacaaaatgt
     8041 tacctattag tctggtgcaa tccctactac gtttcggcga acgtgtccat cttgtccagt
     8101 tacaaaaaac agggaaaaac gcattggatt tttacctgtc ctattacctc ggacaaatta
     8161 ccgccacaga ccccaatgcc caaatcggca tactctcgcg tgatggagga tacgatgttc
     8221 tggtcgaaca tattttgaaa aaccaccagg cgaagggtat cgtgcgccta gccaatatag
     8281 atgaagtaca acatcagaaa attactaccg aaccgccgtc agcattgctg gaaaacactc
     8341 ctcagcctga aaccaccctc aaaccacagc aaccattaac ttcctatttc caagcagccc
     8401 taactgcact gcgccgcccc gacgctttcc gcccctgccg cctgcataac ctgcgacaaa
     8461 atctgcgtaa gcatattttg agtgatttgt ttaaagaaaa aaccgatgaa gaatgcgaaa
     8521 taaccactgc taacgttatc aataaactca aagcacaaaa cttcatcagc attgatgaac
     8581 aggaaaccgt ttcctaccat ctcagtgata atgatttgtt acaaagaatc caacgccata
     8641 ttttaagcca acgtcccaaa acctacgctg attttcaagc cgtcgtgcaa aaccgagcag
     8701 atgcacttca cttaacagtc ggtaccaacg acattcaatc ctttgcgcga catttgcgcg
     8761 accaaaacct gatccgccaa aacaatggga aaattgaata tgcaccgttt actgaaccta
     8821 aaccacagcc aacgcccaag cagcctaaaa aaaccgcatg ggaacctgat gaaattattt
     8881 ggaaaaaagt gattgccgcg ttatcgttaa agaaccgtcc taataaaacc aaaactttac
     8941 gcaatacaat ccaggcactc acaaaatcca atgcacaaga aactgacaaa ctgctacaac
     9001 atttacaaga tacccaagtc ctacgtattg acggtactaa aattgtgtat atcaagtagg
     9061 atgggcattt atgcctgacc tacaaaacca aaaacaacct accaccctta atcaactcca
     9121 caaacccttt tcagacaacc tcgttttttg aaaaacaatc tgtaaacaga tagctgctga
     9181 agaataccgt tgccgagccc caaaacccgt cctgcaactt ttattgtgaa cttcccatta
     9241 tgaaaaaatc ccttttcgtt ctctttctgt attcgtccct acttactgcc agcgaaattg
     9301 cttatcgctt tgtattcgga attgaaacct taccggctgc aaaaatggcg gaaacgtttg
     9361 cgctgacatt tgtgattgct gcgctgtatc tgtttgcgcg ttataaggcg acgcgtttgt
     9421 tgattgcggt gttttttgcg ttcagcatta ttgccaacaa tgtgcattac gcggtttatc
     9481 aaagctggat aacgggcatt aattattggc tgatgctgaa gagattaccg aagtcggcgg
     9541 cgcgggcgcg tcgatgttgg atcagttgtg gctgcctgcg ttgtggggcg tgttggaagt
     9601 catgttgttt tgcagccttg ccaagttcca ccgtaagacg catttttctg ccgatatact
     9661 gtttgccttc ctaatgctga tgattttcgt gcgttcgttc gacacgaaac aagagcacgg
     9721 tatttcgccc aaaccgacat acagccgcat caaagccaat tatttcagct tcggttattt
     9781 tgtcggacgc gtgttgccgt atcagttgtt tgatttaagc aggattcccg cctttaagca
     9841 gcctgctcca agcaaaatca ggcagggcag tgttcaaaat atcgtcctga ttatgggcga
     9901 aagcgaaagc gcggcgcatt tgaagctgtt tggctacgga cgcgaaactt cgccgttttt
     9961 aacccggctg tcgcaagccg attttaagcc gattgtgaaa caaagttatt ccgcaggctt
    10021 tatgactgca gtgtccctgc ccagtttttt caatgcgata ccgcacgcca acggcttgga
    10081 acaaatcagc ggcggcgata ccaatatgtt ccgcctcgcc aaagagcagg gctatgagac
    10141 gtatttttac agtgctcagg ctgaaaacca aatggcaatt ttgaacttaa tcggtaagaa
    10201 atggatagac catctgattc aaccgacgca gcttggctac ggcaacggcg acaatatgcc
    10261 cgatgagaag ctgctgccgt tgttcgacaa aatcaatttg cagcagggca agcattttat
    10321 cgtgttgcac caacgtggtt cgcacgcccc atacggcgca ttgttgcagc ctcaagataa
    10381 agtattcggc gaagccgata ttgtggataa gtacgacaac accatccaca aaaccgacca
    10441 aatgattcaa accgtattcg agcagctgca aaagcagcct gacggcaact ggctgtttgc
    10501 ctatacctcc gatcatggcc agtatgttcg ccaagatatc tacaatcaag gcacggtgca
    10561 gcccgacagc tatctcgtgc cgctagtgtt gtacagcccg gataaggccg tgcaacaggc
    10621 tgccaaccag gcttttgcgc cttgcgagat tgccttccat cagcagcttt caacgttcct
    10681 gattcacacg ttgggctacg atatgccggt ttcaggttgt cgcgaaggct cggtaacggg
    10741 caacctgatt acgggtgatg caggcagctt gaacattcgc gacggcaagg cggaatatgt
    10801 ttatccgcaa tgagtggcgt aaaaaccaat aaagacaaat ttagatgatg tcggggaaga
    10861 tgcccgaccg acaagactat gcaaaatatg aaaaaccaag tacgcggatc aggcatggat
    10921 gcccgatcca atccggtcaa tgtttcagac ggcctgcaaa acagttcggg tcatatcggt
    10981 accaacacgc gttaccgcct gaccaaactc ggcgaacaaa tggcgcgcct gcccatcgac
    11041 ccgaaaatcg cgcgcatttt gctggcggcg aagaaacacg actgcatggc ggaaatattg
    11101 gtgatagcgt ccgcgctgtc gattcaagac ccgcgcgagc ggccgttgga agcgcgcgat
    11161 gccgccgcca aggcacacga gcgttttacc gacaagcaat ccgacttcct tgcctatctg
    11221 aacatttggg acagcttcca gcgcgagcgc gacaaaggtt tgtccaacaa gcagttggtg
    11281 caatggtgcc gtcaatattt cctgtcgcac ctgaggatgc gcgagtggcg cgagttgcac
    11341 caccagcttg cccaaaccgc gattgaaatg ggtttgacca ccaaggaagc ggctttcaga
    11401 cggcctcccg aaatcaggca gctcacgtcg tctgaaagcc aaggcgacca agatttggcg
    11461 gccaaactca aacaaaaaca actggataag aagcaacacc gcgcccaaat ccgcgccgcc
    11521 aaagaagcgg gctacgaaca aatccaccgc gccctgctga ccggccttat cgccaacgtc
    11581 ggcatgaaat cgcccgacgg caacgactac accggcgcac gcggcagccg ttttcatctt
    11641 ttcccggcat cagccctgtt caaagccaag cccaaatggg tgatggcggc agaattggtt
    11701 gaaaccacgc gcctttacgc gcgcgacgtc gccgttatcc agcccgaatg gatagagcag
    11761 gaagcgccgc acctcgtccg ctatcattat ttcgagccgc attgggaaca aaaacgcggc
    11821 gaagtcgtcg ccagcgaacg cgtgacgctt tacggtctga ccgtattgcc gcgccgcccc
    11881 gtgccttacg gcaaagttgc gcccgaagaa gcacgcgaaa tctttatccg cagcgcattg
    11941 gtggcgcagg aatgcgattt gaaagcggat ttttttgtcc acaacaaaaa gctgattaaa
    12001 gaaattaccg aactcgaaca caaatcgcgc aagcaagacg tattggtcga cgacgaagcc
    12061 ctgtttgcgt tttatcacga acggctgccc aatttttata cggcggacgc ggtttcagac
    12121 gacctgcatc ctacaaatcc gcagcaaact gccccctccc ccgtggggga gggctgggga
    12181 gagggcaaaa cagttgccgc acaaaccaac ttttccgcaa ccgcagcaaa ccctctccct
    12241 aaccctctcc cacaggagag ggagcagagt gccgcagctt cagcggtttc aaacgacctg
    12301 catcctgcaa atccccaaca aaccgccccc tcccccgtgg gggagggctg gggagagggc
    12361 aaaacagttg ccgcacaaac caacttctcc gcaaccgcag caaaccctct cccacaggag
    12421 ggggaacaga gtgccgcagc ttcagcactt tcagacgacc ctcaacccca aaagcagcca
    12481 gcatctcaaa aaggccgtct gaaacccctc cccctcgccg acatccgcac cttccaagcc
    12541 tggctcaaaa ccgccgaacg cgagaacccg cgcctgctgt tcctcagccg cgacgatttg
    12601 atgcaacacg ccgccgcaca cattaccgaa gaacaattcc ccaaattctg gcaaaccgca
    12661 gacggcaaat tcaaactcag ctaccgcttc gagccgcacc acccgctcga cggcgttacc
    12721 ctcaccctgc cgctcaccgt gctcaaccgc atcagccccg ccgccctcga atggctcgtg
    12781 cccggtatgt tgcgcgagaa aatccagctg ctgattaaag cactgccgaa gcaaatccgc
    12841 cgcatttgcg tgcccgtacc cgatttcatt accaaatttc tcgaaagcaa tcccgaccgc
    12901 caagcggcca tcattcccca gttggcgcac tttatcgcca aaagcgcagg cgatatgcgg
    12961 attttcgagc aaatcgacca agacgcatgg gcggtgcaag aattgcccga acactgctat
    13021 ctgaatctgc gcattatcga cgacggcgga caagagcttg ccggcggccg caaactgcac
    13081 gaattgcaac aacagctcgg ccaagccgcc gccaccacct tccgcgacaa cacccaagaa
    13141 ttcgagcgcg acaacgttac cgcatgggac atcggcaccc tacccgaatc catcaaattc
    13201 gcacgcggca aacaacagct caccggctat ctcggtctgc aaaaagaaaa agacggccgc
    13261 atcgccctgc gcctgtgtga caccatcgaa gccgccgagc aggcacaccg tcaaggcgtc
    13321 atcgaattga tgaagctgca attaaaagag caggtaaaag acctgaacaa aggcatccaa
    13381 ggcttcaccc aagccgccat gctgctcaaa cacatcaacg ccgacaccct gcgcgacgac
    13441 ctcacccaag ccgtctgcga ccgcgccttt atcggcgaag acgagctgcc gcgcaacgaa
    13501 aaagccttca aagaacaaat caaacgcgcc cgcagccgcc tgcccgccgt caaagaagct
    13561 ctcagtcgtt acctgcagga aaccgccgcc gcctatgccg aactgaacgg caaactcggc
    13621 aaacacccgc tcacccatct cctaagacta cgcctgcaaa ccctgcttgc ccccagcttc
    13681 gccacccgca ccccgtgggc acaatggccg cgcctcccca tctatctcaa agccatgacc
    13741 ctgcgcctcg aaaaatacag cagcaacccc gcccgcgacg cagcccgcga agccgatatc
    13801 caagagctgg aacaaatgtg gcaggaaaaa accgatagct tggtgaaaca aggattgccc
    13861 atttcagacg gccttgcagg gtttaaatgg atgattgaag aattgagggt gtcgctgttt
    13921 gcgcaggaat tgaagacacc gtatccggtg tcggtgaagc ggctgatgaa ggagtgggag
    13981 aggttgaata agtagtgata ttatccaata catttacagg aaaatttcta acttacataa
    14041 aaggtacaac tcaatgaaaa gcgaaaataa ttatgaacgt attgttcaaa attctaaaaa
    14101 attccaacac tctcagaaga atgagatgca gaaacgcata acagatttgc tgcaacaact
    14161 cacaaatggc attcatgaac gcgaccaaat tatggcggtt tccctacttg gtgcgattgc
    14221 cggacataat actttcctat ttggaccgcc gggaaccgcg aaaagtctga tttcccgccg
    14281 tttggcatgt gcatttgaaa atccgcaata ctttgaatac ttgatgaacc gtttcagcac
    14341 acctgaagaa gtattcggac ctgtatctat taaggcattg aaagaagacc gatatctccg
    14401 aaaaatcgaa ggctatttgc caactgccga ttttgcattt ttggatgaaa tttggaaatc
    14461 cagtcctgca attttgaata acctgctgac cattatcaac gagcatattt ttaaaaacgg
    14521 cgatgagagg tttaatgtgc cacttaaagc cctgattgcg gcttccaacg aaattccacc
    14581 tgaaaatcaa ggtttagagg cactgtatga ccgctttatt atccgtctgc ttgtgccgcc
    14641 gattgaacaa gaagagaact tcaaccgttt gctggatagc aaaccaagca gcgacaaacc
    14701 tgaaatcagt gaagatttac ggattagtta tcatgagctt gccaaatggc gtgaacaact
    14761 ccacaaagtc aggctaagta cagatacatt actgattatt aagtatatca gagaagaatt
    14821 agcagcccag tttgataagt taggcgttta tgtatccgac cgacgttggc aacgtgcagc
    14881 cgtattatta aaggcttctg ctttttgcaa tggacgcaaa gaaaccaatc atagcgatgt
    14941 gatcttgctg aaatattgtc tgtggacaac acctgaaaat cgagagtcag tggaacaaat
    15001 tgtgatgaat gccattaaat cctgtgggtt tgatacaggt attgatctgg ctgaattaga
    15061 tagagaaaaa gagagtttgg ataaggaaat aaataaggaa ctttatcatt cacaagatgt
    15121 ttatgagacg gttgaaatga gtggaaaaaa atattttcca gtacaggctg ttttcaaaaa
    15181 cgtacataat agtcatgcta ataaagaact taagatttat gttccatata gcaaatttcg
    15241 tagtaaagaa aaattttatc caatagatag tacaggaaat gaaattcaag aaattgaatg
    15301 tgaatttgat gggcaaggtt gctgtaaatt ggaattgaaa gtgcggggtg gtcactggaa
    15361 tgagcaatat aagccattaa aatttaaacc caatatctta tttcacaaag gagataaaaa
    15421 atcagatgtt aacgaacgtc taattcattc actaacccaa tctatatctg atattcgcaa
    15481 acaattgatt gatacacaga aacaagttga gaaaaaatac gaaaaatacc agcagtacct
    15541 gcaaagcccc tttgttgccc aagcagatat tgatgttgct atatcaggta ttctagacca
    15601 aatcgataaa ctcaatttgc ggattaaaga ctgcgaacgt ttggagtctt tatgtcgtta
    15661 aacagaaaat tatccgaaca aataagatgg attaaagctt ctctacaaat agattatgca
    15721 gatttattta aacagcatgg ttcgttaaat ggacagattc agcaacgttt atcagattgg
    15781 caatatcagg ccaaactggc tttgttgcag aaaaacccat ttatattaca tgaagaaaag
    15841 ctaaaaaaac accaacaaac agaaattttt aatcaacagt ctgttgatga ttatcggtat
    15901 ttttgcaaat tagcaggcgt gccatttgaa aaagaattct ggcagcagga attaacacaa
    15961 agcaaaaatt caaaacccaa taaacagaat ttgcctttga aattgctaac aggaaaatgg
    16021 cagcagcaat tagactatgc caaatctcaa tggtatttaa agcaacttaa tcaattacga
    16081 caggaattta tagataagtt aaaacaggaa ctcgacacta tccgacagct tgcagaacag
    16141 ttagagcaat ttggttttga agcagggctg tggtttgata acagtatcgg caatttaagc
    16201 cagcaaaata ttgaagaaat gaaacgctgg ctgaattact tgacccaaga taagaatgcc
    16261 caacagattg ctgaattact aggcaaaatg cgccaaatcg aacaatctga aaaaatcgaa
    16321 caggtaaaac agactgttta cacaaaaagc ccacaaattg atgtgaattc gcgtgaagaa
    16381 attatcggat tacgtttagg taaagattta gagtatgtat tgccgtctga attggcttta
    16441 atggcagatg aagaaacatc gactttattt gatttgaagt ttttggaatc caaattaatg
    16501 tgttttgaat tgcaaggaat gacctattgc gatgccccaa ctgaaattat agttgaacaa
    16561 aaaagccaag aggatgaaaa gctcggacca atgattttat gtgtagatac cagtggttca
    16621 atggttggtc tacctgaaaa tattgccaaa gcaatggctt tatttttagg agcaaaagct
    16681 aaaagcgaaa accgttcttg ttttgttatc aatttttcca ctggaataga aacttttgaa
    16741 ttaacaagta aaacagggat ttccaatttg attgcttttt tacgtcaatc ctttcatggt
    16801 ggtacagatg ctgccccagc tttacgtcat gcgctaaaaa tgatggagca agaaatctat
    16861 caaaaggcag acttattaat gatttccgat tttgtgatga acagcttacc tgatgatttg
    16921 ttggcatcaa tcgaaataca acgagaaacc ggtaatcagt ttaattcttt ggttattggt
    16981 gatgctttta tgagtaaacg cctaaaaaca cattttgatc gtgagtggat atacaatcct
    17041 aatgttcaga caattcaaga attagtacag ttcaagaaag atattttcaa taaacaagtt
    17101 gtcaactcat aacactatcc tactcatatg tatttttgga agataccacc ctatacaact
    17161 tggaatccat acgcagaata tgtgtggact ttctaaacta ctcccctttc caccctaaaa
    17221 aaggtcgtct gaaacccatt ttcagacgac ctttttccac ttcgcaaaac aagaattccc
    17281 acccctctcc ccaaacgtcg cccgaaccag taaactcccc atatttcaac aacttcttgg
    17341 aagccaacca tgccccgtat ccaccctcct cacctgttcc cgccccatat cgccgaacgc
    17401 ggccttttgt attttcagca gggcaaggtt cgcgatgtcc gaaaaacttc cgccgggcgt
    17461 tatcgggcct ggcgccgttt gctgccggcg gtctgaataa tggtggcgtc aacgacggca
    17521 gcggatgctt tctctacttt taggtttttt tcggtcagtt ggcggttaat cagtttgagc
    17581 aattcggaca gggtgtcgtc ttgcgccagc cagttgcggt aacggcataa ggtgctgtaa
    17641 tcggggatgc tcagttcgtc aaagcggcaa aacaggttga agtcgatgcg ggtgatgagg
    17701 ctgtgctcga gttcgggatc ggagaggctg tgccattgtc cgagcagaac ggctttgaac
    17761 atggacaaca gcgggtaggc gggacgaccg cggtggtctc ggaggtaacg ggttttttga
    17821 cggttcaggt attgttcgat cggttgccaa tcaatcacct ggtccaactt caatagtggg
    17881 aaacggtcga tgtgtttggc aatcatggct tgggcggttt gctgaaagaa ggtgctcatg
    17941 agaaatctcc taaatgtctt ggtgggaatt taggggattt tggggagttt tgcaaaggtc
    18001 tcagtctttt taaaccacag cccttcccaa ctaaaccaaa aggtcgtctg aaccctattt
    18061 tcagacgacc ttttgccact ttgtaaaaca aatcttccca ccatcctctc cccaaacatc
    18121 gcccgaacca gtaaacttct catatttcaa caacttcttg gaagcaaacc atgccccata
    18181 tccacccgct ccacctgttc ccgccccata tcgccgaacg cggactgctc tattttcagc
    18241 agggcaaagt tggtgctgtc caaaaaactt ccaccgggcg ttatcgggcg gaggtatgcg
    18301 gtttggaaaa ctattgggta tggctgaagc tggatagtga tttgtatatt aaagacgaaa
    18361 gctgcgattg cccttatgtc taaaggtgca aacatacctt aaattactat attacatagg
    18421 caaaatacaa gcctataacg aattggaaac aaaatgccgt ctgaaagcat tataaaatct
    18481 gttcaccttt tcagatgagt aatgtacacc cctatacaat ttttgctact ataccccata
    18541 aatccacggc taaagatatc cttattatgt cctatgattt atcgaaacga cttgtaatcg
    18601 gcttagcatc aagtgcccta ttcgacttat ccgaatcgga taatatattt agaatggaag
    18661 gggcagaaac ctataggcaa tatcagagag aaaaacaaaa ccatccccta aaaaaggcgt
    18721 tgtctttcca tttattaaaa aacttctgtc aatcaatgaa ataaacccaa acgacccaac
    18781 gattgggttt attcttttat ccagaaacaa tccagataca gattacgagt cataactata
    18841 ggcttaatat tacacgattc tcattccatc aaggcggaaa accgcacaaa tactgaaaca
    18901 ctatcgatcg atttgtaaac aagcctactt aagtaacttg cagtccttat catttccttt
    18961 aaaataatcc agcccgtcac tacacgaact ggcggacttc ttgcaaataa aggttactag
    19021 attttcattc atcttaataa taaaaggatt tttatcttta tctatggcta ccgccttcaa
    19081 catgaattta ctgtctaagg ccccgcgcgc gattccattc aaacggatac aaaagccttc
    19141 tgcctcttta atcggcaaac ttggccattt ggtagatgtt tgtttaaatc tcccattctg
    19201 cagataaaac ttttccataa aatgtgcatt ttctaacaag gctgcccgca ctgcatttat
    19261 ctttgctttc tcaacataat tgcgatagct cggataaaca attaaagcaa gtacagacaa
    19321 tatcaagacc actgatatta attcaaccag cgtaaacccc cgattatcag tcattacttt
    19381 acttccaata agaacagatt attcaacata tttctttgaa cagacttact atcccattca
    19441 gcagtatgca tatttcccac
//
LOCUS       DAWHXS010000035        18983 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWHXS010000035 DAWHXS010000000
VERSION     DAWHXS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.035"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(105..1892)
                     /gene="lpdA"
                     /locus_tag="WIE22_000920"
     CDS             complement(105..1892)
                     /gene="lpdA"
                     /locus_tag="WIE22_000920"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ4127024.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1969..3552)
                     /gene="aceF"
                     /locus_tag="WIE22_000921"
     CDS             complement(1969..3552)
                     /gene="aceF"
                     /locus_tag="WIE22_000921"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ4127025.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAASAAAAPVAAGGATVQV
                     AVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAVFLKVG
                     DKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPSAPAAAKID
                     EAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAA
                     AGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEAD
                     MTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFN
                     IGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSL
                     GGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAG
                     MRFTVFLAKLLKDFRRITL"
     gene            complement(3702..6365)
                     /gene="aceE"
                     /locus_tag="WIE22_000922"
     CDS             complement(3702..6365)
                     /gene="aceE"
                     /locus_tag="WIE22_000922"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ4127026.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVVT
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7059..8771
                     /locus_tag="WIE22_000923"
     CDS             7059..8771
                     /locus_tag="WIE22_000923"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ4127027.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     LLNG"
     gene            8834..9490
                     /locus_tag="WIE22_000924"
     CDS             8834..9490
                     /locus_tag="WIE22_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ4127028.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9500..9955)
                     /gene="ruvX"
                     /locus_tag="WIE22_000925"
     CDS             complement(9500..9955)
                     /gene="ruvX"
                     /locus_tag="WIE22_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ4127029.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9948..10496)
                     /locus_tag="WIE22_000926"
     CDS             complement(9948..10496)
                     /locus_tag="WIE22_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ4127030.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10498..11058)
                     /locus_tag="WIE22_000927"
     CDS             complement(10498..11058)
                     /locus_tag="WIE22_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ4127031.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11386..13176
                     /locus_tag="WIE22_000928"
     CDS             11386..13176
                     /locus_tag="WIE22_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ4127032.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTA
                     PATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13303..14787
                     /locus_tag="WIE22_000929"
     CDS             13303..14787
                     /locus_tag="WIE22_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ4127033.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15116..17143
                     /gene="uvrB"
                     /locus_tag="WIE22_000930"
     CDS             15116..17143
                     /gene="uvrB"
                     /locus_tag="WIE22_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ4127034.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIAQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVMRDRIRNIKESLLFGAE"
     gene            17189..18457
                     /locus_tag="WIE22_000931"
     CDS             17189..18457
                     /locus_tag="WIE22_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HEZ4127035.1"
                     /translation="MKLSKTRIDKSGLALAKQQYKDEIDFIELEECFDLYRQSHLSPL
                     SKTTIDIQNILREYIGEYYIAQRLKRKPQIIRKLNRLSVRLTQLQDIGGCRIIVPTNR
                     DVDKIYKFLSSKKDNLDFLIKKVTDYREKGRDDSGYRALHIIIERENLNFELQIRSRI
                     QHYWAESIEKTSVVYGYYLKEQEGDRDVINYFKELSDVFYEIESGRKPTTIQKLNLED
                     LRKRCEVIIQKSDTHKILNSYVNEGIISALIEKEKRNTNSLNNWILIFDWNAGVFVSW
                     DVVAQDPKQAVEVYVNYENQYPSLEGFEVVLIGSSDIASVQKTHSHYFGIASYENILE
                     TLDESIFCFAKKDDIDIGARRILAAMVRHKFWGTKTVTSETLKNHFCQNLFGFDYSLR
                     DLIRKELIKYGRDKDLSLNIAKKMEIESYL"
     gene            18862..>18983
                     /locus_tag="WIE22_000932"
     CDS             18862..>18983
                     /locus_tag="WIE22_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4127036.1"
                     /translation="MKYRRFYRNGGTYFFTVATNKRQRILTDDAVRLALRQAVMA"
BASE COUNT         4812 a         4658 c         5068 g         4445 t
ORIGIN      
        1 gtcggagaag atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcatttgctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgccg ccataccgat ggattcgccc
      181 aaggtcgggt gcgggtggat ggttttgccg atgtctgccg cgtcgcagcc catttcgatg
      241 gcaaggcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcggcatc aaaaatcagc ttggtaaagc cgttgtcgca accgttggca
      361 atcgcacggc cggaagccgc ccacgggaag ttggctttgg tgattttgcg gccggatgct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgccc
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tggccggcgc agttttcagc agcaacgtgg
      541 ccttcgtgaa cggctttgtg tgccaacatt ggttgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cattggtacg catttgtttg tccacttcga tgaagccgcg atcggtaacg
      661 gcaacgcctg ctttttccgc gctgatgagt ttgccgttgg gcgcgcggcc ggcggcaacc
      721 aatacggcat cgtaacgttg cggctcttta ggcgcatttg caccttcaaa ggtaacgtaa
      781 acgccgtctt ctttcggctc gactgcaacg gttttggtgt tgaccataat gttgtcaaaa
      841 cggtattcgt tttgtttttg ccatactttc accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtagtg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cgggtctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaactac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca aggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgtt cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccgg gaccgccacc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcagca gctttagggg cttcttgcgc cggggcggca
     1621 gcaggtgctt cagctttggg agcggctgcc gtgccttcag cttcaacgac gacaatcaag
     1681 ccgccttcgg agattttgtc gccgactttg actttcactt ctttgaccac gcccgcaact
     1741 tcggcaggca cgtccatcgt cgctttgtcg gtttccaggg taatcagggt atcgtcaacg
     1801 gcgatggtgt ctcctacgtt cacttcaact gcgataatat ctacattttc gtgtccgcca
     1861 atgtcgggca ctttcaattc aactaagctc atgtctcatc tttctgaatg atgttcaaac
     1921 ttccttttca gacggcacag gccgctatgc cgtctgaagc cgatcgggtt acagggtaat
     1981 gcggcggaag tctttcaaca gcttcgccaa gaatacggta aagcgcatac cggccgcacc
     2041 gtcgatgacg cggtggtcga aagacaggct caacgggcac atcaggcgtg gggcaaattc
     2101 tttgccgttc caaaccggtt tgatttggga tttgcacacg cccaagatgg cgacttcggg
     2161 agcgttcaca atcggcgtga aacctgtgcc gccgataccg cccaagctgg aaatggtaaa
     2221 gcacgcgcct tgcatttctt gcggtttgag cttgccttcg cgggcttttt tagacaattc
     2281 ggtcagctct tggctgattt gtttcaagcc tttttgatcc acgtctttga tgacgggaac
     2341 aaccaagccg ttcggcgtat cggctgcgaa accgatgttg aagtagtttt tcagcaccag
     2401 gttgtcgccg tccagagaag cgttgaattc ggggaaggct ttcagcgcgg aaacggaggc
     2461 tttgatgatg aacgctagag gagacagttt cacgccttcg cgttcccatt ctttgttcag
     2521 ttgtttgcgg aattcttcca gctcggtcat atctgcttct tcgtgtacgg taacgtgggg
     2581 aatcaccacc cagttgcgag acaggttttg accggagatt ttcttaatgc gggacaattc
     2641 tttaacttcg acattgccga atttggagaa gtccacttta ggccacggca gcaagtccag
     2701 accgctgccc aaagatgcgc cggctgcggc aggttttgcc gcgccaccct gcattacgga
     2761 tttcacaaag gctttgatgt cgtcgcccac gatgcggcct ttcaagccgg tacctttgac
     2821 ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt gcggaaggac cggcgtgtgc
     2881 tttggcgaaa gcggcctcgt cgattttggc agcggcaggt gcagaaggtg caggcgcggc
     2941 ggcgggtgcg gcggcagcag tcggaggcgg agcggcagcc ggtgcggcag cttgagcggc
     3001 ttgagcaggg gctgctgcgg cagagccgac ggtttctact tcgataatgg cagagccttc
     3061 ggacactttg tcgccgactt ttaagaatac ggctttaacg acaccggcag cggtacaagg
     3121 tacgtccatt gtcgctttat cggtttccaa agtaatcagc gtgtcgtctt cggcaacggt
     3181 gtcgcccact ttgatttcaa cggcgattac atccacatcg gtatggccgc cgatatcggg
     3241 aacggctact tgaacggttg caccgcctgc ggcaacgggt gcggcagcag ccgaggcggc
     3301 ttcggcagcg gcggcaccgg tttcaacggt caaaattacg ccgccttcgg agattttgtc
     3361 gccgactttg acttttactt ctttcacgac accggcagca tcggcaggca catccatcgt
     3421 ggctttgtcg gtttccagtg taatcagggt gtcgtcaacg gcgatggtgt cgcccgcttt
     3481 aacttctacg gcgatgatgt cgacgttttc gtgaccgccg atatcgggga ctttgatttc
     3541 tacgatactc atttgagttc ctttaatctt gttcagttgg atgccgtctg aaagctgctt
     3601 caggcggcat cggtacggtt tgtctgctca aaccattcgg gcattcggtt ttcagacggc
     3661 ctgatgtcgg aatggggcaa acagaccgtc tgaaacatca atcagcgttt ccagctagga
     3721 gctgaatcgg ctttgatgcc gtatttctca atggcttgtt gaacggtttc tttgctgact
     3781 ttgccttgtt ccgccaatgc gctcaatgct gccacggcaa cgttgtagcg atccacttca
     3841 aagaagcggc gcaggttggc gcggctgtcg gagcggccga agccgtcagt acccaagacg
     3901 tggtagtcgt tcgggatgta ggcgcggata cggtcagcat agctgcggat atagtcggta
     3961 gcggcaataa ccggaccgtc atgaccttgc aatttggaag taacgaaagg tactttttcg
     4021 gcttccagcg gatgcaggcg gttgaagcgt tctacctcga cagcgtcgcg gtgcagcagg
     4081 ttgaaggatg ggcaagacca aatgtctgct tccacgccga agtcggcttt cagcagctcg
     4141 gcaccggtaa ccacttcttg caaaatcgta ccggaaccca tcagttggac tttcttgtcg
     4201 cctttgccgc cggctttcag caggtacata cctttcaaga tgtcttgttc cgcaccttcg
     4261 ggcatatccg gatgggtgta gttctcgttc atcagggtga tgtagtagaa cacgtcttca
     4321 ttattggcat acatacggcg cagaccgtct tgtacgatga cggcgacttc gtattggaaa
     4381 gtcgggtcat aagatacgca gttcggaatc aggtcggcct ggatgtggct gtggccgtct
     4441 tcgtgttgca ggccttcgcc gttcagcgtc gtacggccgg cagtaccgcc cagcaggaag
     4501 ccgcgcgcgt gcatatcgcc cgccgcccaa gccaagtcgc cgatacgttg gaaaccgaac
     4561 atagaatagt aaatgtagaa cggaatcatc gcgaagtcgc tgttggcgta gctggttgca
     4621 gccgcaatcc agtcggccat cgcgcccggt tcgttaatac cttcttgcaa gatttgaccg
     4681 tcaacggatt ctttatagaa catcagttgg tctttgtctt gaggggtata ttgctgacct
     4741 ttcggattcc aaataccgta ttggcggaac ataccttcca tgccgaaagt acggctttcg
     4801 tcgggaacga taggtacgac gcgtttgccg atttttttgt ctttcagcaa agtggacagg
     4861 atgcggacga atgccatcgt ggtcgagaac tcgcgttcac cgctggattt gagttgtgcg
     4921 tcgaatgctg acagctcggg cacttccaat acttcctgcg tcggtttgcg ttgcggcagg
     4981 tagccgccca aagcatcgcg gcgtgcgtgc aggtatttgt attcttccgt atcgggggca
     5041 aaagtcaggt aaggcagatc gccgctttcg atttgttcgt cggtaaccgg aatgtcaaag
     5101 cggtcgcgga attgtttcag ggacgctttg tccatttttt tggcttggtg ggcaacgttc
     5161 tgaccttcgc cggatgcgcc cataccgtaa cctttaatgg ttttcgccaa gatgacggta
     5221 ggtttgccgt cggcatggtt cgctgcgcgg tcgtaggcgt tgtacacttt ttgcgggtcg
     5281 tggccgccgc ggttcaatgc ccagagttgc tcatcggtca tatcggcaac caatgctttc
     5341 agttcgggcg tattgaagaa gtgttcgcgc acatacgcgc cgtctttgga tttgtaagtt
     5401 tggtagtcgc cgtccaaaca ttcttccata cgttggcgca ggataccgtc tttgtctttc
     5461 gccaagaggc ggtcccagcg gcggccccaa atgactttga cgacattcca gccggcgccg
     5521 gcaaagttgc cttccaattc ttggatgatt ttgccgttgc cgcgtaccgg accgtccaag
     5581 cgttgcagat tgcagttgat gacgaaaatc aggttgtcca agccttcgcg tgcagccagt
     5641 gcgattgcac cttgagattc gggttcgtcc atttcgccgt cgccgcagaa acaccatact
     5701 ttacggcctt tggtttttgc caaaccacgc gattccaagt atttcaggaa acgcgcctga
     5761 taaatcgcca tgatgggccc caagcccatg gatacggtcg ggaactgcca aaagtcgggc
     5821 aagaggtggg ggtgcggata ggaaggcaga ccgtgtccgt ccacttcttg gcggaagttg
     5881 ttcagctggt cttcggtcag acggccctcg acgaatgcgc gtgcatagat gcccggggcg
     5941 acgtgacctt ggaagaagac caaatcgcct tcttcgcctt cgcctttggc tttccaaaag
     6001 tggttgaaac cgacttcgta catggtggcg gcagattgga aagatgcgat gtgcccaccc
     6061 agttccaaat ctttcttgcc ggcgcgcaat acgatggcgg cggcgttcca gcgcacgaat
     6121 gcgcggatgc ggtgttcgat gttttggtcg cccggaatgc ctttttcgtt ttcaaccgaa
     6181 acggtattca aatacggtgt ggtcgtgccg tgtggcatac gtacgccctt gtcgcggcag
     6241 tatttgacca ggttttccaa gaggtattgc gcgcgttcgc cgccttcata ttcgaggacg
     6301 gagcttaacg cgtccagcca ctcttgggtt tcgatagggt caacatcgtg taattgggtg
     6361 gacatagtat ctatccttta tgttgagtgt ttaatcggca gggagggtgt agccaacccc
     6421 tttattttcg tttgtgaaaa ataataagca aaaacgaaaa tttctaaatc aaaccgaaaa
     6481 aaataattta agtttaatgt tttcaaaaaa ttataaaagt tttggaaaca agggaaaact
     6541 tgaaaaaaat gttcgatctg ctccacacga aatcctatca gcaatcgggt ctgctggcaa
     6601 ataaccatgc ctgtaaaagc aggttttata ctgaagttgg cgtttggtaa ggtcaagtct
     6661 ttttgatttc gaaatattct gtttgatttt ggcgatattt tttcggaatt gtggtatatc
     6721 gaatcattat gtctctgtcg gcaattttcc tattgtagaa aatctataca caagctttgt
     6781 ggataaccgt gttgataagc cacggcttgt gttaaaaaag tcttacgcaa caaatgtacg
     6841 cttattttga ttaaaaaata ggcacattta aatctataaa aatcatgtgc ttatgttcaa
     6901 tatgatgttt ctggtcatgt ttttgccgtc tgttatggga tttctcccgc gcttggggca
     6961 gggggtgaat tttggaggtt ggtgcaaaac ctgctttttt cgcgttacaa tctgcacccg
     7021 atttactttt gaatttgcct gtttggaaaa atacttttat gaaagccagc caattcttta
     7081 tctctacttt aaaagaagcc cctgccgaag ccgcgcttgc cagccacaag ctgatgattc
     7141 gcgccggtct gattaaagcc aacgcgtccg gcctttatac ttggatgccg atggggctgc
     7201 gcgtgttacg caaagtcgaa aacgtcgtgc gcgaggaaat ggcgcgcgcg ggcagcgtgg
     7261 agctgctgat gccggtggtg cagcctgccg agctgtggca ggaatccggc cgctgggaat
     7321 tttacggtaa agaactgctg cgcctgaaag accgccacga ccgcgatttc tgcatgggcc
     7381 cgacctgcga ggaagtcatc gccgacatcg tgcgcaaaga aatcaacagc tacaaacagc
     7441 tgccgaaaaa cttttaccac atccaaacca aattccgcga cgaagtgcgc ccgcgtttcg
     7501 gcgtgatgcg cgcgcgcgag ttcgtcatga aagacgctta ttccttccat gccgactacg
     7561 cctcgcttca gacgacctat gatgccatgt atgactctta ctgccgcatc ttcacccgtc
     7621 tgggcttggc attccgcccc gtcgccgcag acaccggcag catcggcggt acgggttcgc
     7681 acgagtttca agtgttggcg gaaagcggcg aagatgttat tgcgtacagc gacacttccg
     7741 attacgccgc caatatcgag ttggcaccga ccttgccgct taaaggcgaa cgcgccgctg
     7801 ctcaggctgt gttgaccaaa gtacatacac caaacgtcaa aaccattgag tctttggttg
     7861 aattcctgaa tattccggtt gaacaaaccc tcaaatccat cgtggtcgaa ggcgaaaacg
     7921 aaggcgaaat cgtcttatta ctgttgcgcg gcgaccatga gttcaacgac atcaaggcag
     7981 aaaaactcgc aggcgtgaga tcgccattga cgatggcaag tcctgccgcg attgtcgaac
     8041 agttcggcgc aaacggcggc tcgctgggtc cggtcggctt tgcaggtaaa gtttatgccg
     8101 atttcgccac tgaaaaaggc gcggactggg tcatcggtgc gaacgaagac ggctaccact
     8161 ataccggctt caacttcggg cgcgatgccg ccgagcctga attcgtcgat ttgcgtaatg
     8221 tggtcgaagg cgacgaaagc cccgacgggc aaggccgtct gaaactggcg cgcggcattg
     8281 aagtcggaca cgtcttccag ttgcgcgaca aatacaccca agccatgaac gtaagcttcc
     8341 ttgacaacaa cggcaaatcg caaattatgg aaatgggctg ctacggcatc ggcatcaccc
     8401 gcgtcgttgc cgccgccatc gagcagaata acgacgaaaa aggcatcatc tggaccaaag
     8461 caatggcgcc gtttgaagtc gttatcgtgc cgatgaacta caaaaaatca gacaccgtac
     8521 gcgaagccgc cgacaaaatc tatgccgaat tgctggcggc aggcgcggat gtgctgctgg
     8581 atgaccgcga cgaacgcgca ggcgtattgc tgaacgattc cgagctgttg ggtattccgc
     8641 accgcatcgt catcggcgac cgcgctttga aagaaggcaa tgtcgaatac gccgaacgcc
     8701 gcaacaacga agcgcaggca gttgcaatcg gagaaattgt tgcgcgtgta acagctttat
     8761 taaatgggta agcttggtca aatgccgtct gaaaataatt tcagacggca tttttatttg
     8821 gggagaaagt gaagtggctt cagtattttt agaaggagaa gcagtcgagg tcggcaatat
     8881 tttctgtatc ggcaggaact atgccgcaca tattgaagag ttaaaaaacg aaataccttc
     8941 cgaacctgtg gtgtttatga agccgtcagg cagcattttg aacagcggcg gtacaatctt
     9001 gctgcccgag tttagtaggg atgtgcagtt tgagtgcgaa ctcgtgctgc tggtcggtaa
     9061 ggattcagac ggcacgggcg agggtaaaga tattttggga tgtgtcgcag gatatggtgt
     9121 agggctggac cttacggcac gggatatcca atgccgtctg aaggaaaaag ggctgccctg
     9181 gctgaaggca aaagggttca ggcattccgc gtgcgtgtcg gattttgcgg cggcaggcag
     9241 aatcggcaat cccgagaatg tgttgttttc attaaaacaa aacggcgtat tgaaacagcg
     9301 cggggatacc ggtttgatga tttacccgat tcgggaaatt ttacacaaac tggcggcaga
     9361 ttacggtttg gggaaagggg atttggtgtt taccggaacg ccctccggcg tgggcgcaat
     9421 cggtgcgggg gacaatttgg cattggagct ggacggcttg gtgcgcgcct ctttcacggt
     9481 agggtgttaa ccgcgccgct taaccctcac gcccgttgaa acattccgcc ggaccgccct
     9541 cgaaaaaacc gtgcaagatg gcttgcgccg ccacttggtc gagcaccgat ttgcgttttt
     9601 tgccgaagac ctgtgcttcc gaaagcaggc tttcggcata gacggacgac agccgttcgt
     9661 caacccaata gacggggaga ttgaacctgc cgttcagcct gcgtccgaac ttgcgcgaca
     9721 ggtgcgtcat ttcatgtttc gtgccgtcgg tatgcacggg cagtccgacg acaaaataac
     9781 gcggctgcca ttcttgaacc agcttggcga ttgccgcgaa cttttcatcg ttgctgccgc
     9841 cggtaacggt gctcaaagga tgggatagcc ctaattccgc gtctccttgt gccacgccga
     9901 tacgcgcttc gccgaagtcg aatgccagtg ccgttccttt tggaatttta tgcatggccg
     9961 gctcccgaaa acagggcgag cgggtcgatg ccgagtttgg cgaatgcggc ggcgtaacgg
    10021 tgttcgtagg ggatgtcgaa caggatgtgt tcgtcggcgg gaacagtcag ccacgcattg
    10081 tcggcaagtt cgcgttcgag ctggcctttt ccccagcttg aatagcctat gctgatcaag
    10141 gctttgtcaa ccgcaccttc gcgtgaaata ttttcaatca cgtctcggga agaagttagc
    10201 gcgatattgt ctgaaacgcc gatactgctt tgccagttgc cgatcggggt atgcacgaca
    10261 taacctcgct cgacttgcac cggcccgccc atcatcacgc tgtcgtgctg catccgcatg
    10321 gggatgtttt tgccggtggc ggaaaaaatc atgtccatcg taatcggaga gggtttgttg
    10381 atggcgatgc cgagtgcgcc gtcttcatcg tgtttgcaga tatagacgac cgattgtgaa
    10441 aaaaacgcgt cttccatatc gggcatggcg acgagaaagt ggttggagag gttcatatca
    10501 gcgtttgtcg ggattttcta tcgggttgga aatcatgcag gcgccggcaa gcaggttgcc
    10561 ggaagtatcg gcttgcaccc catcggtttg cggtatgccg ccgccgaaac aggaaaccgc
    10621 gcttaaggaa tttttcggcg agccttggat gattttatcg ctgtacacca aataggcgaa
    10681 ggttttgcgt ttggggtcgt aataacggac aatctgccgg ctcttgaacg cgaagctcgc
    10741 accgtgtttg aaaacttctt tcggtttgcg cacggcggtt tcgtcaaaag aaatcgaaga
    10801 tgccgtctga acgcacgaaa ccgatgcgtc ggacgcgtcc tcttccaaat tgaccatttc
    10861 cttcaagccg cctttttttg catacgaaat ataacaggca accccttgaa cgtcgggatc
    10921 gtcgaatcct tccacttcga tacggtcgtt tttgcccagt atgttgaaaa cggtacttgc
    10981 ccgtccgatt ttatcggttt cgccgctgcc gcaggcagtc agcaggacgg cggcagacag
    11041 cagtagcagt ctgttcatca aaatttcctt atcggttgaa tccccgtttt ccgtgaaggc
    11101 ggcagggctt gctttgtttg gaggggcgca cgttcccaaa atcgggcaac atacggtatg
    11161 cggcttaatg tgctaccctg tgtgtcggaa cacaatatcc gttgcggcat tgaaaatatg
    11221 acgcacaata taaggctgtt tgagccgatt gcaagtatcc gcgcaaagaa ggcgcaagat
    11281 tgggcaaacg gcattttcag acggcataac tgacagtata atccaaacgt ccggctgctt
    11341 tcgtgcagcc ttcagtatta tccaactttt tccgaaggtc gaaccatgcg ctacaaaccc
    11401 cttctgcttg ccctgatgct cgttttttcc acgcccgccg ttgccgccca cgacgcggca
    11461 cacaaccgtt ccgccgaagt gaaaaaacag acgaagaaca aaaaagaaca gcccgaagcg
    11521 gcggaaggca aaaaagaaaa aggcaaaaat ggcgcagtga aagataaaaa aacaggcggc
    11581 aaagaggcgg caaaagaggg caaagagtcc aaaaaaaccg ccaaaaaccg caaagaagca
    11641 gagaaggagg cgacatccag gcagtctgcg cgcaaaggac gcgaagggga taagaaatcg
    11701 aaggcggaac acaaaaaggc acatggcaag tccgtgtccg gatccaaaga aaaaaacgca
    11761 aaaacacagc ctgaaaacaa acaaggcaaa aaagaggcaa aaggacaggg caatccgcgc
    11821 aagggcggca aggcggaaaa agacactgtt tctgcaaata aaaaagtccg ttccgacaag
    11881 aacggcaaag cagtgaaaca ggacaaaaaa tacagggaag agaaaaatgc caaaaccgat
    11941 tccgacgaat tgaaagccgc cgttgccgct gccaccaatg atgtcgaaaa caaaaaagcc
    12001 ctgctcaaac aaagcgaagg aatgctgctt catgtcagca attccctcaa acagcttcag
    12061 gaagagcgta tccgccaaga gcgtatccgt caggcgcgcg gcaaccttgc ttccgtcaac
    12121 cgcaaacagc gcgaggcttg ggacaagttc caaaaactca ataccgagct gaaccgtttg
    12181 aaaacggaag tcgccgctac gaaagcgcag atttcccgtt tcgtatcggg gaactataaa
    12241 aacagccagc cgaatgcggt tgccctgttc ctgaaaaacg ccgaaccggg tcagaaaaac
    12301 cgctttttgc gttatacgcg ttatgtaaac gcctccaatc gggaagttgt caaggatttg
    12361 gaaaaacagc agaaggcttt ggcggtacaa gagcagaaaa tcaacaatga gcttgcccgt
    12421 ttgaagaaaa ttcaggcaaa cgtgcaatct ctgctgaaaa aacagggtgt aaccgatgcg
    12481 gcggaacaga cggaaagccg cagacagaat gccaaaatcg ccaaagatgc ccgaaaactg
    12541 ctggaacaga aagggaacga gcagcagctg aacaagctct tgagcaattt ggagaagaaa
    12601 aaggccgaac accgcattca ggatgcggaa gcaaaaagaa aattggctga agccagactg
    12661 gcggcagccg aaaaagccag aaaagaagcg gcgcagcaga aggctgaagc acgacgtgcg
    12721 gaaatgtcca acctgaccgc cgaagacagg aacatccaag cgccttcggt tatgggtatc
    12781 ggcagtgccg acggtttcag ccgcatgcaa ggacgtttga aaaaaccggt tgacggtgtg
    12841 ccgaccggac ttttcgggca gaaccggagc ggcggcgatg tttggaaagg cgtgttctat
    12901 tccactgcac cggcaacggt tgaaagcatt gcgccgggaa cggtaagcta tgcggacgag
    12961 ttggacggct acggcaaagt ggtcgtggtc gatcacggcg agaactacat cagcatctat
    13021 gccggtttga gcgaaatttc cgtcggcaag ggttatatgg tcgcggcagg aagcaaaatc
    13081 ggctcgagcg ggtcgctgcc ggacggggaa gaggggcttt acctgcaaat acgttatcaa
    13141 ggtcaggtat tgaacccttc gagctggata cgttgatcgg attgccgaaa cggtaggctt
    13201 tatgcaaatc gtgccgatat ggtgcgctgt tccgtgaagt ttgccgtttg ccgtacctgt
    13261 attttccggt ttttggaaat caagagagaa agttaaagaa gaatgtcgaa acctgttttt
    13321 aagaaaatcg cactttatac tttgggtgca atcagcggcg tggccgtcag tctagcggtg
    13381 cagggttttg ccgccgagaa ggacaggcgg gataacgaag tcctgccggt gcaatccatc
    13441 cgcacaatgg cggaggttta cggtcaaatc aaggcaaact actatcagga caaacccgat
    13501 gccgatttgt ttgaaggtgc gatgaagggt atggtggccg gtttggatcc gcattccgaa
    13561 tatatggata aaaaaggtta tgccgagata aaggagtcca ccagcggcga atttggcggc
    13621 ttggggatgg aaatcgggca ggaagacgga tttgtcaaag tggtttcgcc gattgaggac
    13681 acgcctgcgg aacgggcggg ggtgaaaagc ggcgatttca ttgtgaaaat cgataatgtt
    13741 tcgacacgcg gcatgacggt cagcgaagcg gtgaagaaaa tgcggggcaa gccgggtacg
    13801 aagattactt tgacgctgtc gcgcaaaaat gccgacaagc cgatagtcgt caacctgacc
    13861 cgtgccatta ttaaagtgaa aagcgtccgc catcacctga tcgaacccga ttacggctat
    13921 atccgcgtgt cgcagttcca agagcggacg gtcgaaagcg tcaataccgc cgcaaaagag
    13981 ctggtaaagg aaaataaagg aaaaccgctc aaggggctgg tgttggattt gcgcgacgac
    14041 cccggcgggc ttttgactgg cgcggtcggc gtgtcggcgg catttctgcc gtctgaagca
    14101 gtcgtcgtca gcaccaaggg acgcgacggc aaagaccgca tggtactgaa agccattcct
    14161 gaagattatg tgtacgggat gggcggcgat tcgttggcgg gcattcctgc cgagttgaaa
    14221 accataccga tgacggttct gattaattcg ggttcggctt ccgcgtcgga gattgtcgca
    14281 ggtgcattgc aggatcataa acgcgcggtc atcgtcggta cgcagagctt cggcaaaggt
    14341 tcggttcaga ctttgattcc tttgtccaac ggcagcgcgg tcaagctgac aacggcactg
    14401 tattatacgc cgaacgaccg ttctattcag gcgcagggga ttgttcccga tgtcgaagta
    14461 aaagataagg aacgcatttt tgaaagccgc gaggcggatt tggtcggaca catcggcaat
    14521 cctttgggcg gcgaggatgt gaacagtgaa acccttgccg tgccgcttga aaaagatgcg
    14581 gataagcccg ctgtaaaaga aaaaggtaaa aagaaaaagg acgaggattt gtcttcaagg
    14641 cggattccca accctgccaa agacgaccag ttgcggaaag ctttggattt agtcaagtcg
    14701 cccgagcagt ggcagaagtc tttggggctg gcggcgaaaa agccggtttc aaataaagat
    14761 aagaaagata aaaaagataa gaagtaggtt ttgacgatgc cgtctgaaag gatttcagac
    14821 ggcatattat gtttgaaagg acaggttatg agcgattttt taccggaatt tgaaccgtgc
    14881 ggcatttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa
    14941 tagtacggca aggcgaggca acgccgtact ggtttttgtt aatccactat acttgggtag
    15001 cgattgggtt tcagacggca tacattcagg atttcaccgc cttatgccgt ctgaaagacg
    15061 tttccgaccc atccctacta cacactttgt ccgcaaacgc atcacaggca agattatgga
    15121 agttatccaa tacccaaatt cccccttcaa actccaccaa cccttcccgc ccgcaggcga
    15181 ccagcccacc gccattgcag gcctgctcga agggctttca gacggccttg cctatcaaac
    15241 cctgctcggc gtaaccggtt cgggcaaaac ctacaccatg gcgaacgtca tcgcccaaag
    15301 cggccgcccc gccatcatca tggcgcacaa caaaaccctt gccgcccagc tttacgccga
    15361 aatgcgcgaa tttttccccg aaaacgcagt ggaatatttc gtctcctact acgactatta
    15421 ccagcccgaa gcctatgtgc ccagccgcga cttgttcatc gaaaaagaca gcgcgatcaa
    15481 tgaacacatc gaacaaatgc gcctttccgc caccaaaaac ctgatgacgc gcgacgacgt
    15541 gattatcgtc gccaccgtgt ccgccattta cggtatcggc gacccgaccg agtatcaaca
    15601 aatggtgttg tccgtcaaag aaggcgatac catagcccag cgcgacatca tcgccacgct
    15661 cgtttccatg cagtacgaac gcggcgattt ggacttcaaa cgcggcagct tccgcgtgcg
    15721 cggcgacgtg attgacgtgt accccgccga aagctccgaa aacgccttgc gtatcagcct
    15781 gttcgacgac gaaatcgacc gcctcgatat gttcgacccg ctttcaggca gccttattca
    15841 acgcgtcggc cgctacaccg tcttcccgtc cagccactac gttaccccgc gcgacaccgt
    15901 attgcgcgcc tgcgagtcca tcaaagaaga attgcgcgaa cgcatcgaat ttttcgcccg
    15961 cgaacaacgc cccgtcgagc aacaacgcat cgaacaacgc acccgcttcg acctcgaaat
    16021 gctctacgaa atgggcttct gcaaaggcat cgaaaattat tcccgccact tctccggcaa
    16081 aaaagaaggc gaaccgccgc ccacgctgat ggactatctg cccgacaacg ccatcatgtt
    16141 catcgacgaa agccacgtta ccgttaccca aatcggcggc atgtacaaag gcgacgcatc
    16201 gcgcaaacaa aacctcgtcg actacggctt ccgcctgcct tccgcccgcg acaaccgccc
    16261 gctcaaattc cacgaatttg aaaaagtcat gccgcaaacc gtcttcgttt ccgctactcc
    16321 cgccaaatac gaagaagaac acgccggaca agtggtcgaa caagtcgtcc gccctacagg
    16381 gctggtcgac ccccaaatca tcatccgccc cgtcgccacc caagtcgatg acttaatgag
    16441 cgaaatcaac gaccgcatcc aaaaaggcga acgcgttctc gttaccaccc tcaccaaacg
    16501 catggcggag caactcaccg actattacag cgaactcggt atcaaagtgc gctacctgca
    16561 cagcgacatc gacaccgtag agcgcgttga aattattaga gatttgcggc ttggactgtt
    16621 tgacgtactt gtcggcatca acctcttgcg cgaaggcttg gacatccccg aagtctccct
    16681 tgtcgccatc ctcgacgccg acaaagaagg cttcctgcgc tcgcaccgca gcctgattca
    16741 aaccataggc cgcgccgcgc gcaacgtgaa tggcgttgca attttgtatg ccgacaaaat
    16801 caccgactcc atgaaagctg ccatcgacga aaccgaacgc cgccgcgaaa aacaaattaa
    16861 attcaacgaa gaacacggca tcgtgccgca gcagattaaa aaacaggtca aagacatcat
    16921 cgacggcgtg taccacgaag aagacagcgg caaaggccgt agacaaggca aaaacaaagt
    16981 taaagtcggg gagattcata acgaagaaga cgcgattaaa gaaatcgcca aactggaaaa
    17041 agccatgcag caggcggctc gggatttgca gtttgaggaa gcggcggtta tgcgtgatag
    17101 aatacggaat attaaggaaa gcctgttgtt tggggcagag taatgaaata gtttaatatt
    17161 ctcagcttaa tgtataaagg ataattttat gaaattgtca aaaacaagaa tagataaatc
    17221 tggcttagca ttggctaaac aacaatataa agatgaaatt gattttattg aattggaaga
    17281 atgttttgat ttgtataggc aatcacatct ttcaccatta tctaaaacaa ctattgatat
    17341 tcaaaatatt cttagggaat atattggtga gtattatata gctcagcgac taaaaagaaa
    17401 accacagata atcagaaagt taaataggct tagcgttaga ctaactcagt tgcaagacat
    17461 tggtggttgt agaattattg ttccaacaaa tagagatgtt gataagatat ataagttttt
    17521 atcaagtaag aaggataatc ttgatttttt aataaaaaaa gtaaccgatt accgtgaaaa
    17581 aggtagagat gattcagggt atagagcttt acatataatt atagaaagag aaaatcttaa
    17641 ctttgaatta caaattagaa gtaggataca acattattgg gcagaaagta tagagaaaac
    17701 ctctgttgtt tatggatatt acttaaaaga gcaagaaggg gatagagatg taattaacta
    17761 ttttaaagaa ttatcagatg ttttttatga aattgaatca gggagaaaac caactactat
    17821 acaaaagtta aatcttgaag atttaagaaa aagatgtgaa gttattattc agaaatcaga
    17881 tactcataaa atattaaata gctatgttaa tgaaggtata attagtgcgt tgatagaaaa
    17941 ggaaaagaga aatactaatt cattgaataa ctggatacta atttttgatt ggaatgcagg
    18001 ggttttcgtt agttgggatg ttgtagctca agatccgaag caagccgttg aggtatatgt
    18061 taattatgag aatcagtatc catctttaga ggggttcgag gttgttttga taggatcatc
    18121 cgatatcgct tctgtacaaa aaacacatag tcattatttt ggcatagcct catatgaaaa
    18181 tattctagaa acacttgatg aatcaatttt ttgctttgct aaaaaagatg atattgatat
    18241 tggggcaaga agaatattgg cagcaatggt aaggcataaa ttttggggaa caaaaacagt
    18301 aacatcagaa actttaaaaa atcatttctg ccaaaatctt tttggatttg attattcttt
    18361 aagggattta ataagaaaag aattaataaa atatggaaga gataaagatt tatcattgaa
    18421 tatagcaaag aaaatggaaa tagaatcgta tttataagca agcgcagctt gtatccccat
    18481 caaccgtgtg cgtggctacg ccacacacct tacctgcgta tggcacaaaa actttaaaac
    18541 cgaggtgtag ggtgtgtgcg gtacgcacgc acgcgttctt tgtagtgcaa accactgctt
    18601 caaactttca gacgacctca aacgccgtct gagaatctat ggcataaagg ccgtctgaaa
    18661 aaatggtttc agtacaactg ccactggttg taacttgatt aaaaccaccc tttcagacag
    18721 cctgccgttt tcagcaagcg taggtcggat actcgtatcc gacaaaacct ttaacattcc
    18781 cgtcgttgca atccgttgca gcaatgcccg aaatgtcgga ttcgagaatt cgacctatac
    18841 aatcattccg agtataatac tatgaaatac cgtcgttttt accgcaatgg cggcacttac
    18901 ttttttacgg ttgcaaccaa taaacggcag aggattttga ccgatgatgc ggtgcgtttg
    18961 gctttgcgac aggcggtaat ggc
//
LOCUS       DAWHXS010000036        18935 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWHXS010000036 DAWHXS010000000
VERSION     DAWHXS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.036"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..182
                     /locus_tag="WIE22_000933"
     CDS             <1..182
                     /locus_tag="WIE22_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127037.1"
                     /translation="NYHRYGENDYSTSVGSCINGLRYYPFIREKQQLTQQELVGYHQQ
                     VEQLVQSFVNNPSKK"
     gene            199..474
                     /locus_tag="WIE22_000934"
     CDS             199..474
                     /locus_tag="WIE22_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127038.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKARLMCISVFWIVVFNL"
     gene            complement(561..1694)
                     /locus_tag="WIE22_000935"
     CDS             complement(561..1694)
                     /locus_tag="WIE22_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127039.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLEDLDKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(1814..2308)
                     /locus_tag="WIE22_000936"
     CDS             complement(1814..2308)
                     /locus_tag="WIE22_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127040.1"
                     /translation="MENRFAKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEMFKMSVLEDWEHSGKPLDWKKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            complement(2475..2876)
                     /locus_tag="WIE22_000937"
     CDS             complement(2475..2876)
                     /locus_tag="WIE22_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127041.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(3296..3526)
                     /locus_tag="WIE22_000938"
     CDS             complement(3296..3526)
                     /locus_tag="WIE22_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ4127042.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            complement(3972..4646)
                     /locus_tag="WIE22_000939"
     CDS             complement(3972..4646)
                     /locus_tag="WIE22_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ4127043.1"
                     /translation="MAHIQAGDRVLSKDEASGETGYKPVTTRYGNPYRETVYIKVSDG
                     IGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITFKQQPLKAYNL
                     TVADWHTYFVKGDKAETEGVWVHNDCPYGNLSDNKSVGEGKKFTPAQKKAIIQENMNR
                     NGGVVKSDQSGEVLVRPKKSQKGITPPSNEWQIDHVQAKSKGGSNSYKNAQVLSRREN
                     IKKSNK"
     gene            complement(4811..5356)
                     /locus_tag="WIE22_000940"
     CDS             complement(4811..5356)
                     /locus_tag="WIE22_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ4127044.1"
                     /translation="MITVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGGHYNTVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDSEVGNLSDMNRKPFVW"
     gene            complement(5353..6123)
                     /locus_tag="WIE22_000941"
     CDS             complement(5353..6123)
                     /locus_tag="WIE22_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ4127045.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDEASGKTGYKPVTTRYGNPYQ
                     ETVYIEISDGISNNQTLISNSIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDRAETEGVWVHNDCPYGSSSAKGKSGSERNTAKRGT
                     FRQETLKNAWDLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNR
                     QHSSDRKEQLNDYNKGVSLECRHCNRSGGNKDERFKKK"
     gene            complement(6338..6697)
                     /locus_tag="WIE22_000942"
     CDS             complement(6338..6697)
                     /locus_tag="WIE22_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127046.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVHWKINNGNLIQTTN"
     gene            complement(6918..7373)
                     /locus_tag="WIE22_000943"
     CDS             complement(6918..7373)
                     /locus_tag="WIE22_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127047.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILGTFMYEPPDEF
                     RTEYYESPDSNLLIAVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            complement(7354..7983)
                     /locus_tag="WIE22_000944"
     CDS             complement(7354..7983)
                     /locus_tag="WIE22_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ4127048.1"
                     /translation="MVKTADGYKAIARIRTGDRVFSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPTPLKAYNLTVADWHTYFVKGSQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESRIAELKNKIIPGMPKNERLKIEKTIKNITKNANRKAKGEEHGRRGR"
     gene            complement(8117..8434)
                     /locus_tag="WIE22_000945"
     CDS             complement(8117..8434)
                     /locus_tag="WIE22_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127049.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(8437..9705)
                     /gene="mafB"
                     /locus_tag="WIE22_000946"
     CDS             complement(8437..9705)
                     /gene="mafB"
                     /locus_tag="WIE22_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ4127050.1"
                     /translation="MKPLRRLTNLLAACAVAVAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKNLT
                     KAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGK
                     HISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKS
                     GLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            complement(9709..10650)
                     /gene="mafA"
                     /locus_tag="WIE22_000947"
     CDS             complement(9709..10650)
                     /gene="mafA"
                     /locus_tag="WIE22_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ4127051.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            complement(10769..11869)
                     /locus_tag="WIE22_000948"
     CDS             complement(10769..11869)
                     /locus_tag="WIE22_000948"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ4127052.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTATELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRAVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLGGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAANARQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            12194..13768
                     /locus_tag="WIE22_000949"
     CDS             12194..13768
                     /locus_tag="WIE22_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ4127053.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            complement(14121..15542)
                     /gene="hemN"
                     /locus_tag="WIE22_000950"
     CDS             complement(14121..15542)
                     /gene="hemN"
                     /locus_tag="WIE22_000950"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ4127054.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            15747..16481
                     /gene="fnr"
                     /locus_tag="WIE22_000951"
     CDS             15747..16481
                     /gene="fnr"
                     /locus_tag="WIE22_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ4127055.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(16594..17544)
                     /locus_tag="WIE22_000952"
     CDS             complement(16594..17544)
                     /locus_tag="WIE22_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ4127056.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            17908..18636
                     /gene="rmpM"
                     /locus_tag="WIE22_000953"
     CDS             17908..18636
                     /gene="rmpM"
                     /locus_tag="WIE22_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ4127057.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
BASE COUNT         4486 a         4323 c         4622 g         5504 t
ORIGIN      
        1 ggaactatca taggtatgga gagaacgatt acagcactag cgtaggttcc tgtattaacg
       61 gtttacggta ttacccgttt attcgggaaa agcagcagct cacacagcag gagttggtag
      121 gttatcacca acaagtagag caattggtac agagttttgt aaacaatcca agtaaaaaat
      181 aatttaaagg atcttattat gaatgagggt gaagttgttt taacaccaga acaaatccaa
      241 accttgcgtg gttatgcttt ccgtggcgat acctatggcg gttggcgtta tttggctaat
      301 ttgggtgacc gttatgcgga tgatgctgct gcaattgtcg gtaaggatgc aaacttaaat
      361 ggtttgaatt tatggatgaa aaaaggtgtg gaaaacctat gggatgatac ggtcggtaaa
      421 aaggcccgtt taatgtgtat ttccgttttt tggattgtgg ttttcaattt gtagcgaatc
      481 ggattcggca tatacggtat tgcaaaaagc gtttgactct ccaatgccgt ctgaaaaccg
      541 gtttcagacg gcatttgcgt tcagtgagaa aggtcgcgcc tgccgcccga acgtctcgcc
      601 gcagcctccg cataacggcg cacttctttg tccaaatctt ccaagttcaa aggaaaatca
      661 ggcagtctgt ctccctgttt ctcttcgcgg acaatccgcc cgccatccaa ataccacgtc
      721 tgttgcgcat gataggtctg catatccgct gttacgccat ccgctttcaa tgctaccgtc
      781 gaagattgtg caataaaaag atttccgttt ttcaaataat attcgaaact ctggcgtttt
      841 tttccattgt cgaaactcca atagactttt tgcggcagac cgtccgcatc atagccgacc
      901 acaagactgt tcgccttcat cccccggggc atcaattccc gcatattctg ataaaacaca
      961 gaatcgcgcg agtccgatgc aatccggttg ctctcttcgc ggaagtccca aaccttctgc
     1021 tcatcattcg cgacatcccg gtatttcgcc aaatatacct gggccatctg ataacacccg
     1081 aggcaatgct cataaacatc ttccccgatt ttcccgcgcc ccgacgcatc aaataccgaa
     1141 ccgtccggtt gccaaacaac ccgatattct cctgtcgttt cataattttc tccgtgagcc
     1201 gttccgccgt acacatttac agaaaacgga cgatcgttcc gatacagata ttcggcatta
     1261 acaaacgctt ccggcgagcg ttgcgaaagc gaaaccgcaa ccaaaccgcc ctcgccgata
     1321 tggtaatcca gccaaacctc tttcccatgt tcctgctccg ttacgtgaaa ccatttcgcc
     1381 ttttctttca aacgactgag ccggatagcg agcgcgagat aatccttctc cgactgcaac
     1441 ggaccgtcat ccacagctcc ggcaagactt tcctccgtcc tcatcgattc cttcacgatg
     1501 acaaccgccc tgtcggcatt tcggaacagg cgggcaagtt tcgccacaaa agcattcgga
     1561 ttttcaggta tttcagttgc cgtatcgctc aaaaaccaac gcggattaat ctcataggca
     1621 atacccgttc ccagccaaaa ggcaaataca agtgcaaaaa atgacaacag taccggtttg
     1681 aattttttaa acatatttat ttttcgttta acagaatata tcgattatat cagacgagct
     1741 ttgattgttt gatttttaat ttaatctacg gttgctgccg tactttaaaa acccttatgc
     1801 aggctgcggc ttgttactct tgtttgcttc ggttcaattt tttaactaat tcaaatgctt
     1861 cagatcgata ttgcctttgc cattttttcc aatctaacgg ttttcccgag tgttcccaat
     1921 cttctaaaac cgacattttg aacatttcca tttcccacca atattcggca tataaacgtg
     1981 attgattggg aatgtcggct tctgcccaat cggcaaaatg caaaaaatga gaaatatcgc
     2041 aaactatatt attttcagga acttcttcgg caaatagatg cttgactgac tgtaacgctt
     2101 cggtctgata tttttctgtc catttctccc atttttcagg ctttcccgat atttcccaat
     2161 cttccaatgc ggaagcattc aagatttcca attcccacca gcaatcgtaa taacgagagg
     2221 attggttagg aattttattt tccgcttcgg aaacaaattg ggtaaaacta aaaatatcct
     2281 taacgatttt tgcaaatcta ttttccataa attgctgcct cttccaaatt gcgtgccaac
     2341 aacttcggca aaatcaagcc ggcaagcccc tgcattctgt gccaatcgga gaaaatgccg
     2401 tctgaaagcc gaataccgct tcagacggca ttttgattgt tgggttttta atcagagctt
     2461 tgtttgataa tttgttattt tgaataaaat acaggttctg tcgagctgat aacttctatt
     2521 acagaatcgc taaaaaagaa aatataagcc ttataatatt tcttttcaaa aaaatcaaaa
     2581 ctttcttctt tcaaccagtc catataactg tcatctatta tctcgaaaaa ataatttata
     2641 gcaggatttc tatcccaatc atttttttct ttattttcaa ataaatattc tgaaaatttt
     2701 actttataag attcatctgt tatttgaaaa tgaattatat tgtcaataaa tatttcaata
     2761 tttaattcat aatttcggtc ggatgccaaa atgttcacat cttgttcggt aacacaaatt
     2821 ttgtctacgt aaaatctgtg attattgtta gaaaattgta atggtttaat cttcactatc
     2881 gttttccata tctgattttt gttttcttat tgccctgccg gataaagcag gtctgcctcc
     2941 gtactgtcta tcctcacatt tactttcctg ccatcggctt ttatctcccc ggttttaccg
     3001 tttttgccaa cttcgcggct ttggcggcgg caatgttgaa ggcggcttcg acggtttcgg
     3061 cggcattggg gttgacccgt actcatccgc aaacggcaac acgacggacg gtgtttgctt
     3121 ttcagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaagtg taggattttt
     3181 gaagaactta gttctcaata tcctatttca aatgaaggaa ataggtcgtc tgaaaattaa
     3241 aagcagtttc agacgacttt ttgattgttg ggtttttaaa ccgacctaat atttattaat
     3301 ctgatgtact ttcaattcca aattccttat taataaaaga gatttcttgg gattcttttt
     3361 ccgtaagctg gatattgaaa tcttcaatat aatcacatag cgtttcaaaa gctagcaaac
     3421 gttctgaaaa agtaatgtac tctagtgtgg catcaattag attagcgtca attctatctt
     3481 caagagattt tcctaaattt tttattcgtt cgtctaatgt ttccatcttt taaatactca
     3541 ttatttcata aggttatatt tgagattagt aagcgtgtag gttgggtcga gacccaacat
     3601 tctatgttaa ttgaaggaaa ggtcgtttga aaaactaaac acggtttcag acgacctttg
     3661 attgttggtt ttttaaccta acctacgctt gctaatcaac attccatttt tccgatatag
     3721 tatttctcca agcgtaacca ccaagaggta aattacttaa gagattgact gaggaatcaa
     3781 tagtgacaat ttcttttaaa ctaactatca cagcatctcc ttcatgaatt tcattgccat
     3841 ctaagaattg ccacattcca tcatcagcat catgccatac ttctaagatt ccgttttttt
     3901 agacaaaatt cttctggtgg taataacggc agtattttga ggatcttcaa atttccattg
     3961 catattgata cctatttatt tgatttctta atattttctc ttctggataa aacctgtgca
     4021 tttttataac tatttgaacc tcctttactt tttgcttgca catggtcaat ctgccattca
     4081 tttgatgggg gggtgatacc tttttggctc tttttaggac gaaccaaaac ttctccagat
     4141 tggtcagact tcactacccc tccgtttcta ttcatgtttt cttggatgat tgctttcttt
     4201 tgagctggtg taaatttttt tccttcgcca acagatttat tatccgataa atttccatac
     4261 ggacaatcat tatgaaccca aaccccttcc gtttccgcct tatcgccttt gacaaagtaa
     4321 gtatgccagt cggcaacggt cagattgtag gctttgagcg gctgctgttt gaaggtaatg
     4381 ttttgaaccg tctgttttgc gccgctttcg gaaagcaggg tgtcgccttt tttcagacga
     4441 cctgcctgta tccattttcc ttgactgtaa aacggatgga tgcggttgga aatcagggtt
     4501 tggctgttgc cgatgccgtc tgaaacttta atgtaaacgg tttcccgata cggattaccg
     4561 tatcgggtgg taacgggttt gtatcccgtt tctccgcttg cctcgtcctt ggaaaggacg
     4621 cggtctccgg cttgaatatg ggcaatggct ttgtagccgt ctgccgtttt gactaaagca
     4681 ccgacgtata agggacggag acaggatttt tgaagaactt agttcccaat atcctgtttc
     4741 aaatgaagga aaggttgtct gaaagttaaa cacggtttca gacgaccttt aatcaaattt
     4801 aagaaagccc ttaccaaaca aacggtttcc gattcatatc cgaaaggttg ccaacttcac
     4861 tatccagcaa gaactgctca aaagcattcc aacccttttt ttccaccaat tctgcttcct
     4921 gtttatacaa cgggacaagc aaaggaaaaa cagtattgta gtgtccgcca taacagacct
     4981 gaaaatcatc atcgaaataa aatggggcgg aaacatacag tgcatccatt ttgctacctt
     5041 cgataattac cctgggtaat tgaataattt cacctcttaa aataggttgt ctattgtcaa
     5101 tcaaatattc agccactcct gatagaaaag ctgcaatatt ttctttattc cattcttcag
     5161 aacaggtaaa aattaattca aacctacttt tatagttcaa gtcatatcga ttcaagccta
     5221 aagtagaaaa agtagttacc cctttatatg gttgtgatgg gaagacggaa acgctgagat
     5281 gataacgtcg atccaagcat tttataccgc tttcgatttc acccaaataa tgctctaaat
     5341 gttcgactgt aatcattttt tcttaaacct ttcatccttg ttgccaccag atcgattgca
     5401 atgtctgcat tctaaactta ctcctttatt ataatcattt agttgctcct tacgatcaga
     5461 actatgttga cggttactcc atgatggatt atgacttcca tcccaaccat cttttgtatt
     5521 acgtttctcc ttgttatggg gattaccata tacatcttta ccacatgtag gacatttttt
     5581 tgaattaggc tggctaccat ctgctgccaa atcccatgca ttttttagcg tttcttgtct
     5641 gaaggttcca cgtttagcag tatttctctc actgccactt ttaccttttg cgctagaact
     5701 accatacgga caatcattat gaacccaaac cccttccgtt tccgccctat cacccttgac
     5761 gaagtaagta tgccaatcgg caacggtcag attgtaggct ttgagcggct gctgtttgag
     5821 ggtaatgttt tgaaccgtct gttttgcacc gctttcggaa agcagggtgt cgcctttttt
     5881 cagacgacct gcctgtatcc attttccttg actgtaaaac ggatggatgc tgttggaaat
     5941 cagggtttgg ttgttgctga tgccgtctga aatttcaatg taaacggttt cctggtacgg
     6001 attgccgtat cgggtggtaa cgggtttgta tcccgttttt ccgcttgcct cgtccttggc
     6061 aaagacgcgg tcgccgactc ggatacgggc aatggctttg tagccgtctg ccgttttgac
     6121 caaggtgctg ccgtggaagg agcaggtgta ggatttttaa agaacttagt tctcaatatc
     6181 ctgtttcatt catcaaaatg ccgtctgaaa gctgaatacc gcttcagacg gcattttggt
     6241 ggttgggttt ttaagccaac ctacgcttac tgaaaaccaa attgagtttc agacagtttt
     6301 taggtttggg tgtccaatct aacttatatt tgtccattta attagtcgtt tgtatcaaat
     6361 ttccattatt tattttccaa tgtactttat aactatcttc ataataatct aattcaaaaa
     6421 aacctgatat ttcaatatcc aattccatta ttgttttaat gcatttttca aaataaataa
     6481 tgaaataaga ttttacgcat gcaccaaaaa aaatatagct gctccaatta aaactatttg
     6541 tcgggaaaac ccacccgctt ttatatattt ttgcagattc tttctcttcg atattgaagg
     6601 gacaattatt ccaaaaatta ttaatgtttt cttggatgat ttttatatca tcgtcagagc
     6661 attcaatcca tccttttatg gaaacatatg atgccatgtt taatctccta aacctgtttt
     6721 aacaatgccg ccttttgatt caatatatga cttaacttgt gaatgaacac cgtgcctgtt
     6781 ccgtacagca agcacaggtt ggattaagat tcgacaatct gtttggattg acagaaaggt
     6841 cgtctgaaaa atcgattacg gtttcagacg accttttgtt gttgagttct caacccaacc
     6901 tatgcctgcc attaatgtta ttcatcgtag taaggttctg tcgaataatt gtctttgccc
     6961 ccggcaatga tagtaacaat tttccctttt gcttcccaag cttgtactcc tatttcatca
     7021 aactcataga catatgtcgg ataagattca tttgataaat aatatttatc aacaccgtat
     7081 gatttagggt gatggaaaag ctgtttaaaa tcttcaaaat tcagacctat tatattaacg
     7141 cccataaaat atagctcctg ataacaaaat atcgaaataa ttttgttttt cttgacggca
     7201 attagtaaat ttgagtcggg agattcataa tattctgttc taaactcatc aggaggttca
     7261 tacataaaag ttcccagtat gtttttgtac tgtgttatat ccgcaccaaa acggaatatt
     7321 cctacagaag taaaaggtaa aaattcggga gttttaacga ccgcgtcgac catgctcttc
     7381 tccttttgct tttcgattgg cattttttgt aatatttttg attgttttct caatctttaa
     7441 gcgttcattt ttgggcattc cgggaataat tttatttttt aattcagcaa ttcttgattc
     7501 cgctgatatt tgacttcgac cgccgtctcc atgtttttca ttcttggagc ttcctgttct
     7561 tttaggcgga caagaattat gaacccaaac cccttccgtt tccgcctgac tgcccttgac
     7621 gaagtaagta tgccagtcgg caacggtcag attgtaggct ttgagcggtg ttggtttgac
     7681 aacgatgttg cggacggttt gggttttgcc actttcggat aacagcctgc ttcccgcttt
     7741 caaatcttcc gctttaatcc atttgccgcc cgaataaaac ggatggatgc ggttggaaat
     7801 cagggtttgg ctgttgccga tgccgtctga aactttaatg taaacggttt cttgatacgg
     7861 attgccgtat cgggcggtaa cgggtttgta tcccgttttt ccgcttgcct cgtccttgga
     7921 gaagacgcgg tcgccggttc ggatacgggc aatggctttg tagccgtctg ccgttttgac
     7981 caaggtgctg ccgtggaagg agcaggtgta ggatttttga agaactcagt tctcaatatc
     8041 ctatttcaaa tgaaggaaat gccgtctgaa aattaaacac ggtttcagaa gacattgtga
     8101 ttgttggttt tttaacctac gcttgcgaaa ttaattcctt tctcctgaat actgcaccat
     8161 ttatttggtt taactccata gaagaatgat tagaataaat acattcaaag tcagcttcat
     8221 caaaatattc tccacacata gaccataact ctgtaatcaa atattccata tcttcatggt
     8281 cttctgtgat ggcttctctt gataaataat ttttgatttt taaaaacctt cttccttcct
     8341 cgtaatctac ggtaattaac ctgatatctg agtgtatcat gcccagcatt gctatattca
     8401 taccgactcg cagccagctt ggtaatatat tcataactat tgcacctttt taatggtttt
     8461 agggttagcc ggaacaatgt gaactccatt tttacctgaa tgaatcgtac caaaattggt
     8521 acttaatcct gatttcccat cgctgcctat aggataccca aaatcaacaa caggatttcc
     8581 tcttgctcct ttagaaataa ctggataagc acctgaatgt attccgttca acaattcttg
     8641 aggattaatg ttttgattta aagtactctt accttcaatg tagtttctat gtcctgaaat
     8701 atgtttccct tgagctccat catgaatttt agtcccaata gattttgctt taaaatcaac
     8761 attgcgggtt tgaaacattt ctccatctac agactgagat aatcttgaag cagtgttatg
     8821 cttgtaggag tctgagaaat ccccactaac cgcagccttc cccggttttg ccgcctttgt
     8881 caggtttttg actttggcaa acggcaggac gttgaccagg gcttcgacgg tttcggcggc
     8941 attggggttt tcctgcatcc actggtcaac ggcttcgccc gcgctgataa aggggttaag
     9001 cgcgccggcg gcgacgccgt tgataaactc catgctgttg ccccagcggt cgagcttggc
     9061 attgtgctcg aacatttttc tgttggcttc atcggcgcgg tcggagaaat tgctgccgag
     9121 gttgttgtag ttgtcggata tgcgttgccg gatgctgcgg gtgtcggtcg gattgagttt
     9181 gatgctgcgt gctgtgccgt tgacgtgata ggtgtattcg tcgcgtgcgc ccgtaggttt
     9241 ggggtaattg ccgcccttcg ggccgtcgta ggcatcggcg ggatggtgct cgtgtccttc
     9301 ccagttgagc cggtacacgg taaagccttc gtcaacgttg ccttgttctt tgcttgcgct
     9361 gtcggcggcg tggttgtcga agggggcgtg ttcttcgtgt ccgtgtccgg aaaagcgggt
     9421 gtggtagccg attgtgccgt tgatgtttgc ctgttggatg agcaggttgc ccatctggtg
     9481 ggtatagtct tggatgacgt tgattttgcc ggtgcggtcg gaaacgctgc cgcgcgggtc
     9541 gccgaagagg tggtatttgc cgccgggttc gtagtgctgc cgttgggcgt tatcggtaat
     9601 gaacgggtct tgcgccaagt ccgccgcgag ggcgggctgt ccgaaggcgg cgaccgctac
     9661 ggcgcaggcg gcaaggaggt ttgtcagtct gcgcagcggt ttcacggttt atcctccttt
     9721 gcggcggcgg atgacttcgt tgccgacatc ggggttttta ccgttgtttt gtttgaagtc
     9781 gggacggttt tgggcggttg tgtcgccgta gggggtgatg tcggagaaat cgaccatcag
     9841 gcggtctgag gctttgacgg ttttgctgac tttgtaaggg ccggtccaaa gggcgtattg
     9901 ttcttggtat tgggattcgt aggcggcggt tttaggggca atcagcagtt tccggctgtc
     9961 gcggtcgacg gcgaaatatt cgagcttggt ttgggcttta agggtttcgg cgttgtagag
    10021 gtgcagttcg gtacggctgc ggacggtgcc gaatacgtcg acggttacga atacgtcggt
    10081 gtcggcgtat tcgggcggta cgacttcgat gccgcgcagg tagaagacgg tttggatgag
    10141 gttggtcagg aaggaaacgt cgcgggggtt ggcgagcagg gtttcgttgc ggtagtcgcc
    10201 catgccgttg acggacagtc cggcggagcg ttcgcctttg cgtccgctgt ttctagtcag
    10261 ggcggcggcg ggggcgttca aaagcgatgt ggaagtggtt acgctggaga gcgcgtcgga
    10321 tttggtggtg gcggtagtgt cgtaggcggg gtagctgtat tgggtggcac tttcggggtt
    10381 gttgtggtag ccaccgcgta tcagtgcgtc gatagagtag cgtccgccgc ttatgttgcc
    10441 cgaaccttgg tcgcccataa cggagacgta aagggcggct ttgcgtcctt tcagggcgga
    10501 caaatccatt tctttgacgg cggcgcggga cgatgcggcg acgagttctt gttcgacggc
    10561 aaagcgtttg ccgccgccgt gggcgggtat gccggtcagt gtgccgcagg cggttaggac
    10621 gagggggatg aggaggagca gggttttcat ggcagggttt gttttgtttt gaacgagttt
    10681 gagtgtaaat ggattttaag ggtttgtaaa cgaaagagga aaaaatgccg tctgagcggc
    10741 ggaaatggct ttcagacggt atttgcgctc aataataata tcccgcgccc agaatacacg
    10801 gtttggatgc gccggttgct ttgtgcggac taccgggaat gcgattaatc caacacgccg
    10861 ccaaccacgc aaatgcggcg gcttccaccc attgcggatc gaggttcagg tcggcggtgc
    10921 tgtgcaggga aacgcgtgtg ccgaaacatt ccgccaaatc cgccattaaa acaggattgc
    10981 ggataccgcc gccgcaaatg tacatttgac gggcatttgc cgctgcgtgt gagacggcgt
    11041 cgaaaacggt ttgcgcggta aaacgggaaa gcgtccgcaa tacgtcgtat cggttttcgc
    11101 cgccgccaag gtaggtttcg agccaattta gggcaaacag ttcgcgcccc gtgcttttgg
    11161 ggtggggtcg tgcgaaatac gggtgggcga gcagcctgtc gagcagttgc ggcaatatgt
    11221 tgccttgtgc cgcctttgca ccgtttttgt cgtaaggaag ctgccagtgt gcctgcgtcc
    11281 acgcgtccat cagcatattg cccggccctg tgtcgaagcc gaaggcgggt gcgtcggagg
    11341 ggagtacgct gatgttggca atcccgccga tgttcagtac cgcgcgtgtt tctttgtcat
    11401 cgcggaacag ggctttgtga aaggcgggga cgagcggcgc accttgtccg ccggccgcaa
    11461 ggtcgcggct gcggaagtcg ccgacggtaa aaatctgagt cagttccgcc agcagcggca
    11521 aatcggcaag ctgtatgctg taaccgtgtt ccggtgcatg tcggacggtt tgcccgtggc
    11581 agccgagggc ggtaatgtcg gacggcgcga ggttttgact gcacagcagt tcggtggcgg
    11641 tttgcgcata taggcggctg agttcttgcg acaaaatcct gctgcggtgc agttcgtctg
    11701 tgcctgtgtc ctgcaaatcc agcaattggc ggcgcagcct gtcgggatag ggggtaaagg
    11761 cgtgcccttc cgcgcccagc catttgccgc cgtccatccg taccagcacg gcatccgccc
    11821 catccatact ggttcccgac atgatgccga tgtaaagctg tgtttccatc atcactccca
    11881 aactggtgca aaacgccatt ttaacgtgta ttgacgctcg tataccgatt tgccgccgca
    11941 gtgtaaataa agtgtaaata aatgtttcaa gacggatgga aaaatattat aatgcgcccg
    12001 caacatccag tagtagaagt gtcatacaaa ccgtttccgg cagcagtttt gcattcggtc
    12061 aggtttgggg gtattcggat gcggttcaga aggaaggatg cgtctgccat atcccgaaac
    12121 ggcagttcga ccggaggcag cagtacagtg tcggcaacac tcatgatttc caccacatta
    12181 aaggaagatt gccatggctc aaatccaaat gagcgcaaat gttaaaacca tcaacgccgt
    12241 ctttgccgcc atgctggtag gtacagtcgg ctattttatt tattggggct tgggttatac
    12301 ccattacaat tacgccgcct tattcattat tgccacgatg ttcggcgtgt ttatggcgtt
    12361 caacatcggc ggcaacgatg ttgccaattc tttcggcacc agcgtcggtg cgggtacgct
    12421 gaccatcccg caggctttgc tgattgcggc ggtatttgag gtcagcggcg cggtcatcgc
    12481 gggcggcgag gtaaccaata ccatacgcaa aggcatcgtc gatttgaagg gtgttgattt
    12541 cgaacccata cagtttgtgt ttattatgat gtccgcgctt ttggcggcgg cgttgtggct
    12601 gttgtttgcc tcgaaaaaag ggcttccggt atctaccacc cattccatta tcggcggcat
    12661 tgtcggcagc gcggtatgta tggcggtaat gaacgatgcc gcatcgggcg atttgatacg
    12721 ttggggcaag ctgggcggta ttggtgtttc ttgggtattg tcgcccgtgt tgggcggcgc
    12781 ggtgtcctat ttcctgtttt cgcgcgtcaa gaaaaacgtc ttagattaca acgcttgggc
    12841 ggaaggcacg ctcaagggca tcaagcagga aaaaaaggcc tataaagaac ggcaccgcct
    12901 gtttttcgag ggtttgtccg aagccgaaaa agtcgagtac gccaccaaaa tggcgcacga
    12961 cgcgcaaatt tacgacgaac ccgaattcga tccgcaagag ctgcaatcgg agtattaccg
    13021 cggtctttat gcgttcgaca accgtaaaaa caatgtcgat tcctacaaag cgctgcattc
    13081 ttggattccc tttatcgctt cgttcggcgc gatgatgatt tccgctatgc tgattttcaa
    13141 gggcttgaaa aacctgcatt tggggatgag caacgtcaac agcttcctga ccatctttat
    13201 gataggcgcg gcggtgtgga tggggacgtt tgtgtttgcc aaaagcctca agcgtaaaga
    13261 cttgggcaaa tcgacctttc agatgttttc atggatgcag gtctttaccg cctgcggctt
    13321 cgcattcagc cacggtgcga acgatatcgc caacgccatc ggtccgtttg ccgcgattat
    13381 ggatgttttg cgtaccaaca gcgttgccgc gcaaaatgtc gtccccccga ttgcgatgct
    13441 gactttcggc atcgcgctga ttgtcggttt gtggtttgtc ggtaaagagg tgattaaaac
    13501 cgtcggtacg agtttggccg aaatgcaccc cgcctcgggt tttaccgccg aattgtccgc
    13561 cgcatccgtc gtgatgggcg cgtcgctgat ggggctgccc gtgtccagta cgcatatctt
    13621 ggtcggcgcg gtactcggca tcggtctggt caaccgcaat gccaactgga aactgatgaa
    13681 gcccatcggt ttggcgtggg tcattaccct gcctgccgcc gccgtattgt cggttgtctg
    13741 ctacttggtt ttacaggcag tattctgatt gtaaaatact gatgccgtct gaacccgtgt
    13801 tcagacggca ttttgttgat ggaatgtgcg ggcttgtgcc ttatgcacaa tctgttctgt
    13861 cgggatatgc cgtttggtat agtgattaac aaaaatcagg acaaggcgac gaagccgcag
    13921 acagtacagc tagtacggca aggcgaggca acgctgtact ggtttttgtt aatccactat
    13981 atcttggttt cggaacggtc ggacacaaag gtgcggaacg ttatgatatg ccgccgcctg
    14041 ttcttgaaaa cacttatcct gccggcagca aaatgccgtc tgaaaaagcc tttcagacgg
    14101 catttgtacg ttagacgcaa tcacaccgtt tgcgaatatt tcgccttggt ttctttatgg
    14161 cgcaggtggt aatcgaacac catggcgatg ttgcggatga ggaagcgtcc tttcggggta
    14221 acggtcagcc cgtggctgtt caggcgcacc aatcccaaac cggcgagttt ttccaaatcc
    14281 gccagttcgt ctttgaagta gcggtcgaac gggatgccga acatactttc gtaaatccgg
    14341 tagtcgagcg cgaaacggca catcaaatcc tgaatgatgt tgcggcgcag gatgtcgtcc
    14401 tgattgagct gataaccgcg catgatgggc agtctgcctt cgtcgatggc ggcatagtag
    14461 gcatcgatgt cgcgttcgtt ttgggaatag gtgctgccga ttttgccgat ggacgacacg
    14521 ccgatggcga ccaaatcgca atccgcgtag gtcgaatagc cttggaagtt gcgctggagg
    14581 aagccttctt tgagggcgat ggagagttcg tcgtcaggtt tggcgaaatg atccatgccg
    14641 atgaagacgt agccgcgttc ggttagggtt tggacgcagt attgcagcat atcgagcttc
    14701 tcttcgctgt cgggaacggc ggcggtatcg atgcggcgtt gcggtttgaa cacgtgcggc
    14761 aggtgggcgt agtgataaag ggcgaggcgg tcgggatcga gcgacaaaac ggtatcgatg
    14821 gtggttttga tgctttccga agtctggtgc ggcaggccgt aaatcaaatc gacgctgacg
    14881 gatttgaacc ccgcttcgcg cgccgcatcg atgacttctt tggtttcttc gtaactttgg
    14941 atgcggttga ccgctgcctg cactttgggg tcgaaatcct gaatgccgat gctcatgcgg
    15001 ttgaagccga gtctgccgag catgaggacg gtgtcgcggc tgactttgcg cgggtcgatt
    15061 tcgatggagt attcgccggt ggggattaac tcgaaatgtt tgcgtatcat gcggaagaca
    15121 cgttcgatct gttcgtcgct caaaaaggtc ggcgtgccgc cgccgaagtg cagttgggca
    15181 agctggtgcc gtccgttcag atgtggagcg agcagttcca tttctttttc aagatattcg
    15241 atgtaggcat cggcgcggct tttgtctttg gtgatgattt tgttgcagcc gcagtagtag
    15301 cagatggtgt tgcagaacgg aatgtgaatg taaagggaaa gcggtttgtt taacgcgccc
    15361 ataccgcgca aatgtaaagc tttgatatat tcgccttcgc ggaaaccgtc atggaaacgg
    15421 tcggcggtag ggtaggaagt gtagcgcggg ccgctggcgg gcaggctggc aatcagcgcg
    15481 cggtcaaact cggggcggtc atcgtttaca ttgtgattgt tctgtatctg aatgattttc
    15541 atggtgtgtg tgtgcggttt tatgatgtta gtcaaatttt ggatagtttg gtagaatgcc
    15601 acagtatgat aaacctgtct tgatatgtgt caataagcac atatagtgga ttaaatttaa
    15661 ataaggacaa ggcgaggcaa cgccgtactg gtttaaattt aatccactat aatcatgatg
    15721 gggcaaagcg cacaaaaagg tacggtatgg cttcgcataa tactacacat cagatgaaaa
    15781 cgctgtgttc ttcctgttct ttgcgggaac tctgcctgcc tgtcgggctg ctgcccaacg
    15841 agctcagcca actcgatgcc gtcatccgtc aaagccgccg cctgaaaaag ggcgaatacc
    15901 tgttctgtgt cggcgaagcc tttacctcgc tctttgccat ccgttcgggc ttcttcaaaa
    15961 caaccgtcgc cagtcaggac ggccgcgatc aggtaacggg tttctttatg tcgggcgaac
    16021 tcatcggcat ggacggcatc tgttcccatg tgcacagttg cgacgcggtc gccttggaag
    16081 acagcgaagt gtgcgaactg ccgtttaccc acatcgaaga actggggcag aatatcccca
    16141 gcctgcgtac ccactttttc cgcatgatga gtcgtgaaat cgtgcgcgac caaggtgtta
    16201 tgctgctgtt gggcaatatg cgcgccgaag agcggattgc cgcctttctg ttgaaccttt
    16261 cccaacgcct ttattcccga ggttttgctg ccaacgactt catcttaaga atgtcccgcg
    16321 aagaaatcgg cagttatctc gggctgaaac ttgaaaccgt cagccgcaca ttatctaaat
    16381 ttcatcagga aggattgatt tccgtcgaac acaaacacat caagatcctc aatctgcagg
    16441 tgttgaagaa gatggtatcc ggttgttcgc acgccatttg attaacccgt acgaacattt
    16501 cagacggcat tctcaataaa cacagggcag acgaaaacat ctgtcctgtt tgttgtatct
    16561 gccgcaaagt gccgtctgaa aaccggcagc cgcctaaatc gaaaaatcct cgctgatggg
    16621 cgtgtacaga atcctatcca ccttctcgcg tgtcaggtgc ggcgcaaacg cttggataaa
    16681 gtcgtaggca tatccgcgca aataagtatc gctgcgcaaa gcaatccacg tcggcgacgg
    16741 ctcgaacagg tgtgccgcat ccacaagctg caaatcgccg tccgtatccg ggttgtacgc
    16801 cattttcgcc atcagtccca cgcccaaacc caagcgcaca taagtcttca atacgtccgt
    16861 atccgccgcc gccaatgcga catccggctg ttccaaacgg gctttggaaa atgcccgcgc
    16921 gatgctgctg cccgcattga atgcaaattc ataagtaatc agcggaaacc tcgccaaatc
    16981 ttcaatgcgg agggggtttc tgcattcgag caaggggtgg tcgttcggta cgataaccgc
    17041 atgagtccag tcatagcagg gaagttttcc cagttcggga tggtcgtcta tccgttccgt
    17101 aacaatcgcc aagtccgcct cgcctgaggt aaccatacgt gcgatggcgg cagggctccc
    17161 ctgtttgatg gtcaggttga ctttcggata gcgtttcaca aaatcggcaa caatcaaggg
    17221 tagggcatag cgtgcctgag tatgcgtcgt ggcaaccgtc agcgaaccgc tgtcctgtcc
    17281 ggtaaactcg ctgccgatat ttttaatgtt ctgtacatcg cgtaaaatac gttccgcaat
    17341 atccaaaacc accttgcccg gctgcgagac cgaaaccacg cgcttgccgc tgcggataaa
    17401 aatctgaatg ccgatttctt cttccagcaa tttgatttgt ttggagatgc cgggttgcga
    17461 agtaaacaag gcttcggccg cttcggaaac gttcaggttg tgctggtaaa cttctaaggc
    17521 gtatttcaat tgttgtaatt tcatggcggg tcggtgtggg tctgtgtcgg gtggctgaac
    17581 attgtttata atttatcata ttttcttgcc ggtacggtat ggggctttgc cgttgtgttt
    17641 gttgtttttg tgcaacggca atcgtgcgat atggaaaaaa tccccctaaa gtaatgacac
    17701 ggaattgatt tttcggcatg atagactatc aggaaacagg ctgttttacg gttgttttca
    17761 ggcgttgagt attgacagtc ctccccctgc ttctttatag tggagactga aatatccgat
    17821 ttgccgccat gtttctacag cggcctgtat gttggcaatt cagcagttgc ttctgtatct
    17881 gctgtacaaa tttaatgagg gaataaaatg accaaacagc tgaaattaag cgcattattc
    17941 gttgcattgc tcgcttccgg cactgctgtt gcgggcgagg cgtccgttca gggttacacc
    18001 gtaagcggcc agtcgaacga aatcgtacgc aacaactatg gcgaatgctg gaaaaacgcc
    18061 tactttgata aagcaagcca aggtcgcgta gaatgcggcg atgcggttgc tgcccccgaa
    18121 cccgagccag aacccgaacc cgcacccgcg cctgtcgtcg ttgtggagca ggctccgcaa
    18181 tatgttgatg aaaccatttc cctgtctgcc aaaaccctgt tcggtttcga taaggattca
    18241 ttgcgcgccg aagctcaaga caacctgaaa gtattggcgc aacgcctgag tcgaaccaat
    18301 gtccaatctg tccgcgtcga aggccatacc gactttatgg gttctgacaa atacaatcag
    18361 gccctgtccg aacgccgcgc atacgtagtg gcaaacaacc tggtcagcaa cggcgtacct
    18421 gtttctagaa tttctgctgt tggcttgggc gaatctcaag cgcaaatgac tcaagtttgt
    18481 gaagccgaag ttgccaaact gggtgcgaaa gtctctaaag ccaaaaaacg tgaggctctg
    18541 attgcatgta tcgaacctga ccgccgtgtg gatgtgaaaa tccgcagcat cgtaacccgt
    18601 caggttgtgc cggcacacaa tcatcaccaa cactaaggct aggcaatatc ttgccgatgc
    18661 atgaggttag tggattttgt accaggtact gttgcaatat tcgtgaaacg tcggtcggca
    18721 tcgatgatgt gaaacaaacc cccgcttttg cggggtttgt ttttttgggt ggttttctga
    18781 aacggctatc gtcagaatcg gggtgcaggt tcggattcgg attcggattc ggattcggat
    18841 tcagattcag attcagattc agattcagat tcagattcag attcagattc agattcagat
    18901 tcagattcag attcaggttt gtgtcccatt gccgc
//
LOCUS       DAWHXS010000037        18398 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWHXS010000037 DAWHXS010000000
VERSION     DAWHXS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18398
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.037"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            719..2167
                     /gene="gnd"
                     /locus_tag="WIE22_000954"
     CDS             719..2167
                     /gene="gnd"
                     /locus_tag="WIE22_000954"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ4127058.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYSDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2230..3501
                     /gene="waaA"
                     /locus_tag="WIE22_000955"
     CDS             2230..3501
                     /gene="waaA"
                     /locus_tag="WIE22_000955"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ4127059.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3542..3991
                     /locus_tag="WIE22_000956"
     CDS             3542..3991
                     /locus_tag="WIE22_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ4127060.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4024..4998
                     /locus_tag="WIE22_000957"
     CDS             4024..4998
                     /locus_tag="WIE22_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ4127061.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5067..5142
                     /locus_tag="WIE22_000958"
     tRNA            5067..5142
                     /locus_tag="WIE22_000958"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5100..5102,aa:Lys,seq:ttt)
     gene            5362..6615
                     /gene="murA"
                     /locus_tag="WIE22_000959"
     CDS             5362..6615
                     /gene="murA"
                     /locus_tag="WIE22_000959"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ4127062.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6696..7874
                     /locus_tag="WIE22_000960"
     CDS             6696..7874
                     /locus_tag="WIE22_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ4127063.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMEKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7941..8189)
                     /locus_tag="WIE22_000961"
     CDS             complement(7941..8189)
                     /locus_tag="WIE22_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ4127064.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(8280..9197)
                     /gene="ftsX"
                     /locus_tag="WIE22_000962"
     CDS             complement(8280..9197)
                     /gene="ftsX"
                     /locus_tag="WIE22_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ4127065.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLTRDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPDQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9194..9844)
                     /gene="ftsE"
                     /locus_tag="WIE22_000963"
     CDS             complement(9194..9844)
                     /gene="ftsE"
                     /locus_tag="WIE22_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ4127066.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10039..10521)
                     /locus_tag="WIE22_000964"
     CDS             complement(10039..10521)
                     /locus_tag="WIE22_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ4127067.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGKVNEKSLTDAVKLAHSKCR
                     "
     gene            10645..10998
                     /gene="arsC"
                     /locus_tag="WIE22_000965"
     CDS             10645..10998
                     /gene="arsC"
                     /locus_tag="WIE22_000965"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ4127068.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10995..11651
                     /locus_tag="WIE22_000966"
     CDS             10995..11651
                     /locus_tag="WIE22_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ4127069.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIIFTGGRSKKGYM
                     TEAEVGRRYALKQGIPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11767..13161
                     /locus_tag="WIE22_000967"
     CDS             11767..13161
                     /locus_tag="WIE22_000967"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ4127070.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13182..13577
                     /locus_tag="WIE22_000968"
     CDS             13182..13577
                     /locus_tag="WIE22_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127071.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDYAWNAVYGAGGTKASGN"
     gene            13581..14072
                     /locus_tag="WIE22_000969"
     CDS             13581..14072
                     /locus_tag="WIE22_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4127072.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14195..16789)
                     /gene="mutS"
                     /locus_tag="WIE22_000970"
     CDS             complement(14195..16789)
                     /gene="mutS"
                     /locus_tag="WIE22_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ4127073.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCTTHMGSRLLALWLHHPLRNRAHIRARQEAVTALDSQYEPL
                     QCRLKNIADIERIAARIAVGNARPRDLAALRDSLFALSEIDLSTNGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17233..18237
                     /gene="gap"
                     /locus_tag="WIE22_000971"
     CDS             17233..18237
                     /gene="gap"
                     /locus_tag="WIE22_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ4127074.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTSDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
BASE COUNT         4207 a         4488 c         5306 g         4397 t
ORIGIN      
        1 cagagcggtt tctgtttttt ccgataaatt cctaaaactc aaaatttcat cattcccact
       61 ttcgtgggaa tgacgggatg taggttcgtg ggaatgacgc ggtacaggtt tccgttacgt
      121 atccccatcc gaaactttac ttatacacaa gcaacccaaa gttatctgac tgaaatattg
      181 tgagatttac ccgcaaatct cattttttcc accacttata cacaaattcg gatgcgcact
      241 ctgccgatat gcagaaataa gacattcgtg ccgacacctg accatttcca caagttccaa
      301 acacgcttcc ggcttaaatc tccacagtat tcccgttctt aaaggcgcca gcagccattc
      361 tgtaaagaaa agtaggatgc catctgcaca acaagcaaac tatccacaaa acttattccc
      421 cataaaaaac aatccacagg tatgcacaaa agggaatccg ccttaccgac accctaaaaa
      481 aaagaaatcc tgatgatttt taaaataaaa aaaagttacc cacagaaaat ttctcctatc
      541 aatataatca tcgttttatt atttaaattt tatattttat ttataaccct tcagcaaaac
      601 ggtacagcaa agcgtaaagg catcttttca aaaaattaaa aagttaagga ttgttaaaca
      661 agtgtaaatc gggtagagtt gcggtcgata aattaaatct atgacggagc ttcctacaat
      721 gaaaggcgat attggcgtaa tcggtttggc ggttatgggg caaaacctga ttttgaatat
      781 gaacgattgc ggatttaagg ttgtcgccta caaccgaaca atcggtaagg tggacgaatt
      841 tttaaacggc gcggcaaaag ggaccggtat tatcggcgcg tactccctgc aagacttggt
      901 cgataagctg gcaaaaccaa gaaaaatcat gatgatggtt cgcgcaggtt cggtggttga
      961 cgactttatc gaacaactgc ttccgctgtt ggaagaaggc gacatcatta ttgacggcgg
     1021 caatgccaat tactccgaca cgacacggcg gacacattac cttgccgaaa aagggatttt
     1081 gtttgtcggg gcgggcgtat ccggcggcga agaaggcgcg cggcacggcc cgtccattat
     1141 gccgggcggg gataagcgcg cttgggaagc cgtcaaaccg atttttcagg cgattgccgc
     1201 caaaacgccg cagggcgaac cgtgttgcga ctgggtcggc aaggacggcg cggggcattt
     1261 tgtcaaaatg gtgcataacg gcatcgaata cggcgatatg cagttgattt gcgaagcgta
     1321 ccagtttatg aaagacggtt tgggactgtc ttacgacgaa atgcaccgcg tgtttgccga
     1381 gtggaacaaa accgagctgg attcttatct gattgaaatc acagcggcaa ttttgggcta
     1441 taaggacgaa ggcggtgaac cgctggtcga gaaaatcctc gatacggcag ggcaaaaagg
     1501 cacgggcaaa tggacgggca tcaatgcctt ggatttgggt attccgttga cgctgatttc
     1561 cgaagcggta ttcgcccgct gcgtttcgtc gttcaaagaa cagcgcgtgc agaccggcaa
     1621 gctgttcgcc cgaaccgtta cgcccgtcga aggcggcaaa caagaatggg ttgaggcgtt
     1681 gagacaggcc ctgcttgcat ccaaaatcat ttcctacgca caaggcttta tgctgatccg
     1741 cgaagcgggc gaaagctacg gctgggattt ggactacggc aacactgcgc tgctgtggcg
     1801 cgaggggtgc atcattcgca gcgcattttt aagcaatatc cgcgatgcct atgaaaacaa
     1861 tcccgatttg gtgttcttgg gtgcggatcc gtatttcaaa aatattttgg aaaactgcct
     1921 gccggcgtgg cgcaaggtgg ttgccaaggc ggtcgaatgc ggcatcccta tgccctgcat
     1981 ggcttcggcg attacgttct tggacggcta cacgaccgaa cgtctgcctg ccaacctctt
     2041 gcaggcacag cgcgactact tcggcgcgca cacttacgaa cgcaccgaca aaccgcgcgg
     2101 cgagtttttc cataccaact ggacgggcaa gggcggagat accgcttcga caacctacga
     2161 tatttgattc cgttgccgaa atgccgtccg aaccgttcgg acggcatttg ttttgcagga
     2221 aatagaaaga tgttccaatg gctttatgat gtattgtggc tgcttgcgcc gatatggata
     2281 cggcgttatt tggacaaacg ctccggaagt gccccggcat atcgggcgca tcggggcgag
     2341 cgtttcggca agccgtatcc gaatcccgtt accggcgcgg tttggataca cgccgtttcg
     2401 gtcggggaaa cgcgtgccgc ccaacctttg atacgcgagt tgcggcagcg ttttcccgat
     2461 gcgccgctgc tgatgacgca gatgaccccg acgggacggg aaaccgcgca agttctgttt
     2521 cccgatgcgc aatgccgcta tcttccgtat gacaaaaaaa cgtgggtacg gcagtttttg
     2581 cgcgaacacc gcccgatgtt cggcgtattg atggaaacgg aaatctggcc caacctaatg
     2641 aaggaatgcc ggcgcgcggg tgtgccgctg tttttggcga atgcgcggct gtcggaaaaa
     2701 tcgttgaacg gttatctgaa agtccgccgc ctgatccgtc ctgccgccgc ttcgctgacg
     2761 ggttgtctgg cgcagacgga ggcggatgcg gcgcggttgg cgaaattggg cgcggcatcc
     2821 gtgcaggtgt gcggcaatac caaatacgac ctgatgccgt cggaacagat gaaaacgctg
     2881 gcggggcagt ttgaaaaacg catcggaggc agaccggttg ccgtgtgcgg cagcacgcgc
     2941 gtttatcggg gcgaagacga ggcggaaaaa ctgctggcgg cgtggcaaca atatcgcggc
     3001 gatgcgctgc tggtcgtcgt gccgcgccat cccgagcatt ttcagacggt atttgaaacg
     3061 gcaaaacgct tcgggtttaa ggttcagcgg cgcagcgacg gtttgccggt cgaacctgat
     3121 acgcaggtgt ggataggcga cagtatgggc gagctgtatg cgtattacct gtgcgccgat
     3181 gtcgcttttg tcggcggcag tctggtcgat tcgggctgtc agaacattat cgaaccgctt
     3241 tcctgcggcg ttccgacgat attcggcttt tcaacctaca atttttccga agcctgccgg
     3301 cacgccttgg catcgggggc ggcggttcgg gttgagtcgg cggacgcgtg gcgggaagcc
     3361 gttgaaaaaa ccttatcgtc cgaggggggc ggaatgcaga tgcaggcgca cgtggacggc
     3421 tttatcgcac aacatcgcgg agcgggcgcg agaatcgccg aggcggtacg ggaagcggta
     3481 tgcggacatc ggggacgata gtgatacaat ccgtatccga gttttccggt tggagcatcg
     3541 tatgagtatt tatgccgtcg cgcacatcgt ccacctgtat tgcgccattg cctttgtcgg
     3601 cggcgtgttt tttgaagtgc tggttttgtc cgtcctgcat acgggacggg tgtcgtgcga
     3661 ggcgcggcgc gaagtggaaa aggcaatgtc ttaccgcgcc gtcagggtga tgccgtttgt
     3721 ggtcggactg ctgttcgcca gcggcgtcgt gatggcggca aaccgctatc tttctatatt
     3781 gggcgaaccg tttgccactt ccttcggtac gatgctgacg ctgaaaatcc tgttggcgtt
     3841 cagcgtgttg gcgcacttcg ccatcgccgt cgtcaaaatg gcgcgttcca cactgaccgt
     3901 cggctggtcg aaatacatac acgccgtcgt ctttacccat atgctgctga ttgtcttttt
     3961 ggcaaaagcg atgttttata tcagctggta acacaattac atttcaaatc aggagattga
     4021 accatgacag aatatcccgg catcgggtcg ccgttgtttt acggcgtttt ttttgcggca
     4081 gtgctggtca tgattgcctt ggatatgttt tcgctgaaga aaaacggcag ccataaagtc
     4141 ggcgtcaaag aagccttggc atggagcggc ttgtgggttg ccgtatcctg cctgttcgcg
     4201 ggctggctgt atttcgaact cgccggcaat ctcggctacg gcgcggcagt cgcaaaagaa
     4261 aaagtattgg aattctttac cggctacatt ttggaaaaat cgttggcagt cgacaatatt
     4321 ttcgtgttcc tgatgatatt cggctacttc aaagtcgcac cacagtttca gcaccgcgtg
     4381 ctgctgtacg gcgtattggg cgcattggta ttgcgcactg tcatgatttt cgtcggcgcg
     4441 gcactggttc agcagtttga gtggattctg tatctgtttg gcgcgttcct gctctatact
     4501 ggtatacaca tgatgaagcc cgaaggcgat gagaaagagg atttggcaaa cagcaggctg
     4561 ctgaatgccg tcaagaaagt cgttccggtc ggcacggaat ttcacggcga gaaatttttt
     4621 accgtcgaaa acggcaaaaa aatcgccacg ccgctgtttt tggtgctggt catgattgaa
     4681 ttgagcgatg tcgtgtttgc cgtggacagt attcccgccg tctttgctgt taccaccgat
     4741 ccgtttatcg tgctgacctc caatattttc gctattttgg gtttgcgggc gatgtatttc
     4801 ctgctggcgg atgtggcgga acgctttatc ttcctgaaat acggcttggc attcgtgttg
     4861 agttttatcg gtgtgaaaat gctggtaatg cattgggtgc atatcccgat ttccgtttcg
     4921 ctgtcggtcg tgttcggcgc gttgggtgca tcgatactga cctcgttaat ttatacgaaa
     4981 aaacagcctg ataaataaaa tgccgtctca acgccgacaa aatggttgac agcatcaggc
     5041 ttgatgtata gaatttcgag cttgtcgggt cgttagctca gccggtagag cagcggactt
     5101 ttaatccgtt ggtcgagcgt tcgaatcgct cacgacccac cagataaaac aaaaaaagct
     5161 gagttttcaa aactcggctt tttttgtttt ccgacaatcc ttgttcggaa agcgcggcat
     5221 ttcgtcaaat tctttggtgc ggcttccgta tttttcccga aaatccctta taattccgcc
     5281 tctgcaacct tcatcgggtg atgctttatg cgttgtcccg agaattgaaa tattacaagt
     5341 acccaccagg aaagaacaaa cgtggacaaa ctgaaaatct ccgcaaacgg cccgctcaac
     5401 ggggaaataa cggtctcggg cgcgaaaaac gcggcattgc cgctgatgtg cgcgggtttg
     5461 ctgacatcgg gtacgttgcg cctgaaaaac gtccctatgc tggcagatgt caaaaccacg
     5521 caaaaactgc ttcaggggat gggcgcgcgc gtcctgaccg acaatatcag cgaatttgaa
     5581 atcaacggcg gtacggtcaa caacacgtgc gccccttacg agttggttcg gacgatgcgc
     5641 gcttcgattt tggtgctggg cccgacgctg gcgcgtttcg gcgaggcgca agtcagcctg
     5701 ccgggcggct gcgccatcgg ttcgcgcccc gtcgatcagc atttgaaagg cttggaagcg
     5761 atgggggcgg aaatcgtcat cgaacacggt tacgtcaagg ctaagggtaa actcaaaggt
     5821 acgcgcgtgg cgatggatgt cgttaccgtc ggcggcacgg aaaacctgct gatggcggcg
     5881 acgctggcgg aaggtacgac ggttttggaa aactgcgcca tcgagcctga agtggtcgat
     5941 ttggcggaat gcctggttaa aatgggcgcg aaaatcagcg gcatcggaac atcgaccatg
     6001 atcgtggaag gtgtggacga gctgcacggc tgcgaacaca gcgtcgtccc cgaccggatc
     6061 gaggcgggga cgttcctgtg cgcggtggcg ataaccggcg gcagggtggt tttgcggaat
     6121 gccgccccga aaacgatgga agtggtgttg gacaaactgg ttgaggcagg tgcggtgatt
     6181 gaggcgggcg acgattggat tgccatcgat atgcggcagc gtccgaaggc ggtggacatc
     6241 cgcacggtcg tccaccccgg cttccccacg gatatgcagg cgcagtttat ggcattgaat
     6301 gccgtggcag agggaagctg ccgcgtggtg gaaacgattt ttgaaaaccg ctttatgcac
     6361 gtccccgagt tgaaccggat gggggcgaac atcacaaccg agggcaatac ggcatttgtg
     6421 cagggtgtgg aacagctttc cggcgcggtc gttaaggcga cggatttgcg tgcgtccgca
     6481 agcctcgtta tcgccggttt ggcggcgcgc ggcgaaaccg tggtcgaaca gatttaccac
     6541 ttggatcgcg gttatgaaaa tattgaaaaa aaactcggca gcgtcggcgc gaaaatcgag
     6601 cgcgtatccg gctgatcggg ttgcgatgcc gtctgaagcc ttcagacggc atcaattgtt
     6661 tctgtatcgc aaacactgat tgaaaaggac tgattatggc atttttaaaa ctgaccgaac
     6721 aaaacgtgca gggcaaaacc gtcctcatcc gcgccgatat gaacgtgccg ttcaaagacg
     6781 gcaaaatcag cgacgacacc cgtatccgcg cctcgctcgc gtccatccaa tactgcatgg
     6841 acaacggcgc atccgttatc gtgatgaccc acttgggccg cccgaccgaa ggcgagtttc
     6901 accccgaaga cgacgttgcg cccgttgccg cgcatttggg cggcctgttg ggcaaaaacg
     6961 tgaaagtatt gaacgactgg cgcgaaaaca aaccggcttt gaacgcgggc gatgtcgtga
     7021 tgctgcaaaa cgtgcgtatc aacaaaggcg agaagaaaaa cgatttggaa ctgggcaaag
     7081 cctacgcttc tttgtgcgac gtgttcgtca atgacgcgtt cggcaccgcg caccgcgccc
     7141 aagcctcgac cgaagccgtc gcccaagccg ctcccgttgc ctgcgccggc gtattgatgg
     7201 cgggcgaact cgacgctttg ggcaaagcct tgaaacagcc cgcgcgcccg atggttgcca
     7261 tcgttgccgg cagcaaagtg tccaccaagc tgaccatcct cgaatcgctg gcggacaaag
     7321 tcgaccaact catcgtcggc ggcggtatcg ccaacacctt cctgttggcg gaaggtaaag
     7381 ccatcggcaa atctttggcg gaacacgatt tggtggaaga atccaaaaaa atcatggaga
     7441 aaatggcggc caaaggcggt tctgtgccgc tgccgaccga tgtcgtcgtt gccaaagcct
     7501 ttgctgccga tgccgaagcg gtcgtgaaag acattgccga cgttgccgaa gatgagatga
     7561 ttttggacat cggcccgaaa tccgccgccg cgcttgcgga gttgctcaaa gccgccggca
     7621 cggtcgtatg gaacgggccg gtcggcgtgt ttgagtttga ccagttcgca ggcggtacga
     7681 aagcccttgc cgaagccatt gcccaaagca aagcattctc gattgcaggc ggtggcgaca
     7741 cgctggcggc gattgctaaa ttcggcgtta ccgaccaaat cggctatatt tctaccggcg
     7801 gaggcgcgtt ccttgagttc ttggaaggca aagagctgcc tgccgtagct gctttggaaa
     7861 aacgcggcgc gtaagcggtt tgatataaaa aatgccgtct gaaacgttgt gctgtttctg
     7921 acggcatttt tgtgtggcgg ttattgtgct ttggcattcc attctgccgg agtggcggca
     7981 accgaaatgg aaagtgcgtg cagttcgtta ctttccagtt gggctttgag tccgtcttta
     8041 atcaggcggt ggcgcgcgag gcgtgccttg ccttcaaatt ctgatgaaac gatgacggcg
     8101 aaaaaatggt gtccgtcgcc ttctacttcg atatgttcgc attttgccac gccttcaatc
     8161 atggctttta cttgttccga ggtcagcatg gtaggttcct tgttggaaaa atgcgccatt
     8221 atactggcaa attgaaatgc cgtctgaaaa cgggttcgga cggcattttt gacggtgttt
     8281 tattttttgg ctttgaagcc gagcaggtgc tgggtggtgg caagccacgc gccgaatacg
     8341 cccaacgcga tgacgaagcc gaacaccagt gcgatttctc ccgggtagaa gaaacgccag
     8401 ccgatattaa gtccgtaggg tttgaagatg gcatcgacca aggggcgcac ggcagagagc
     8461 agccaaccgc aaagccccaa gctgacggcg gcggaaagga tgctctgcca catggcttga
     8521 taaaggaatg ggcggcggat aaacgacgcg ggcgcgccca agagtttggt gatttcgatt
     8581 tcttctttgc ggctgaggat ttgcagtcgg atggtgttgt gcgcgacaag gacgaacgcc
     8641 atccccagcg tcagggaaag aaaccataag attttacgga tgaattcgtt gatttggtac
     8701 agcgtttgca cccattcggt atccatagac gcggattcga ccataggcag tttggtaatg
     8761 tctcggtaga ttgcctgcat ttggtcggga gtggttgccg ggtcgggggt aacgataaag
     8821 acatccggca gggggttacc gtcaagcatg gaaatcagat tttggtcgag attggactgt
     8881 aattcttcca aaccgtcttc tttgctgata aagcggatgt tgtcgagtcg tttgtcgcgc
     8941 gtcagcaggc tgcgcacggt atcgctgtcg ctttgtgcgg cggcggtttc catatagatg
     9001 gtgatttgcg gcgactcgtt gagtttgcct aaaacgcttt gtccgctttg gatgcccaaa
     9061 tacataaaca gcggcagggt catggcgacg gcgagcatta tgagggtaag cagtgtgccg
     9121 aaaggttggc gcagaagctg tttgagcgcg gtgcgcgcgg attcgacgtg cagcgagagg
     9181 tagtggatga tgctcatgcg agtcgtcctt tcgagaggcg caggatgcgg tgtccgtagt
     9241 ccgccatcag ggtttcgtca tgtgcggcga cgatgacggt tgttcccgct tcgtggaagg
     9301 ttttgaacaa ttccataata tcgagcgcgt aggcgcggtc gaggttggcg gagggttcgt
     9361 cggcaatcag caggccgggc tggtgaacga cggcgcgggc gatgcacagg cgttgttgtt
     9421 caccgccgga gagggttacc gggtcgtcga gttcgcgtcc tttcaggccg actttttcga
     9481 tggcgatgcg ggcacgctct tcggctttgc gcggcggata gccgataatc cgaagcggca
     9541 ggatgacgtt ttgcaggacg ttgcggtcgt agaggatttt gtggtcttgg aacacgatgc
     9601 cgatgtgttg gcgcataaag ccgatttggt tgtcggacaa tgtgccgaga tcctgcccgt
     9661 taaacaggat tttgcccctg ctcggcttgg tgatgcccga aatcagcttg aggacggtgg
     9721 atttgcccga accggagtgt cccgcgataa atatcatttc gcctttgttg atttggaagc
     9781 tgacgttttt cagggcttca aaaccgccgg gataggtttt ggaaacttgt tcgaaacgga
     9841 tcataggagg tcctgtaagg aatgggcggc atatcgtttc gggcggttgc gtccgcaggg
     9901 atatgccgtc tgaacaatag gtaagacagt gccgattata aagattccgc ctgtttttag
     9961 atagtggata agtttggcgg atatgaaggt cgggcgggaa aaatgccgtc tgaagcggat
    10021 tcagacggca tccggcgttt aacggcattt tgaatgggcg agtttgacgg cgtcggtcag
    10081 gcttttttcg ttcactttcc cggtaatggt ctgcctgtat ccgcatttcg gtgcttcgac
    10141 gacggtaaag ggcagtacgc cgacagtgtt tccgtaggtt ttcataaagt ttcggctgtt
    10201 cgccccggtg taacgccaaa tcgggtagga aacaggagtt tgtttgagga agttgccgat
    10261 attgtcggat gtgtcgagcg cgatgccgac catatcgacg ctgccttttt tctgcgcttt
    10321 gtaccatttg gacatggcag gcatctcttt tcggcacgga ccgcaccagg ttgcccaaag
    10381 gttgacgatg cgtacggggg ctttgagtga ttgcaggctt tgcggggtgt tgtctttcca
    10441 tccggccagt tcgtctgccg aggcggtatg tgcggcaaat gtggcggcaa gggtgattgc
    10501 ggcaagattc aagtatttca tcgttttgtc ctttgtatgt cgattcgggt attgtaaacc
    10561 gtttggtacg gttgtaggcg ggatattgag taaaaccctg tgtaagccga taataagggg
    10621 attgtcgaga agtaaggaaa tgtcatgtct gaaatcaaaa ttttccacaa tccgcgttgc
    10681 agcaaatcgc gtgccgcctt gtccctattg gaagaacgcg gcattgctgc cgaagtggtc
    10741 aaatatttgg atacgccgcc cgacttgtcc gaattgaagg atattttcaa caaattgggt
    10801 ttggcatcgg cgcgcgggat gatgcgcgtg aaagatgatt tgtacaagga attgggtttg
    10861 gacaaccccg atttggacaa tgacgcgctg ctgcgtgcca tcgccgatca tcccgccctg
    10921 ttggagcgtc cgattgtttt ggcgaacggt aaggcggctg tcggcaggcc gttggaaaat
    10981 atcgaagcgg tattgtgaac aaacggctgt tttgcagccg caacggcttg cgctattacc
    11041 tgttgggcgg tttctgtttg agtgtttttc cgcttttgct ggtgtttgcc tcatccgttt
    11101 gggcggttta tcggacgggc gggcaggttt tgccgccgta tgtccgggcg gatgccgcgc
    11161 tggtgttggg cgccgccgcg tgggacaaac gcccttcccc ggttttccgc gaacgcatca
    11221 accacgccat cgcgctttac caaagccgca gggtaggcaa aatcattttt accggcggca
    11281 ggagtaaaaa gggttatatg acggaggcgg aagtcgggcg gcggtacgcg ctcaagcaag
    11341 gaattccggc gcgcaacatc ctgtttgaaa acacttcgcg caacacttat gaaaacctga
    11401 acaacatccg tccggttttg cgtgccaacg gtattgccag cgtggtgatt gtcagcgatc
    11461 cctaccattt ggcgcgggcg gcggaaatgg cggaggattt gggtgtccgc gtttatatgt
    11521 ctgccacgcc gaccacgcgc tttgatgcgg gaaataagaa aaaaatcttt atgctgcaag
    11581 agggatatgc gttatccctc taccgtttgg aaaaatgggg cagccgcttt ttgggatggc
    11641 tgtctgattg aagcggtaga aaatgccgtc tgaaacttat ccgcaggcag ggttcggacg
    11701 gcatttttaa tgcccaagca cggtaaaaac agtaaaataa gccgttttaa agattttgga
    11761 gccatcatga ccgtcaaaac ccgtttcgcc cccagcccaa ccggctacct gcacatcggc
    11821 ggcgtacgca ccgccctgtt ttcttgggcg tttgcccgcc atcataaagg cgaattctta
    11881 ttgcgcatcg aagacaccga cttggcgcgc tctactgccg aatccgtcaa catcatcctc
    11941 gacggcatga aatgggtcgg actcgattac gacaacgccg gcaacgtcgt gtaccaaacc
    12001 cgccgtttcg accgctataa agaagtcatc gccgaacttt tagaaaaagg cgatgcctac
    12061 tactgctatt gcagcaaaga agagttggaa gccatgcgcg agaaagccga aaaagaaggc
    12121 acggcgactt acgaccgccg ctggcggccg gaagcaggca aaaccctgcc cgaaatccct
    12181 gccggcgtgc aacccgtcgt ccgcttcaaa acgcctttgg atggcgtaac caagtggacg
    12241 gacttggtca aaggcgaaat ctccatcccc aacgaagcac tcgacgacct gattatcgcc
    12301 cgcgccgacg gcacgccgac ctacaacttc tgcgttgtcg tggacgacta cgacatgggc
    12361 gttacccacg ttatccgggg cgacgaccat gtgaacaaca ccccgaaaca aatcaacatc
    12421 ttaaaagcca tcggcgctac cctgcctgaa tacggccacc tgccgatgat tcttaacgaa
    12481 caaggcaaaa aaatctccaa acgcagcggc gataccgttg ccattaccga tttcggcgcg
    12541 atgggcatcc tgcccgaagc catgctcaac tatctggcac gtttgggctg ggcgcacggc
    12601 gacgatgagt tcttcacgat ggaacaattt atcgaatggt ttgatttgaa agacgtttct
    12661 ccatctccaa gccgcatgga tttgaagaaa ctctactgga tcaacggcga acacatcaaa
    12721 atcacgccca acggcaaact cgccgaactc gtcaaaccgc gtctcgctct gcgcgatatc
    12781 catgaaactg aaaaacctgc attggaagac gtgttggcac tggtcaaaga ccgcgcccaa
    12841 gacctgaaca cacttgccga cgagtgcctc tacttctacg tcaaacaaac gcctactgaa
    12901 gcggacgtgc agaaacactg ggacgatgaa gccgccgccc gtatgctgcg ctttgccgaa
    12961 cgcctcgaag ggctggaaga ctggaatgcc gaagccatcc acgacctctt caaacccttc
    13021 tgcgacgaag aaggcattaa aatgggcaaa ctcggcatgc ccctgcgcct tgccgtctgc
    13081 ggtacggcga aaacccccag cgtcgatgct gtgttggcat taatcggcaa agaagaagtg
    13141 ttgaaacgca tccgcgctta agttatccac aggcgtgggc gatgccgtct gatgtcggaa
    13201 tgcggcttca gacagcattc aaacggaagt tgaacatgaa aaaaatattg tattttctga
    13261 tgtttgtttt ttctacaagc gtatgggccg gggatgcgga agacaatctg ctcagcatcc
    13321 aatccggcta tcgtgcctta ctgcaaaaac aaaacaatct ggacggaaaa atcatcggga
    13381 tgcagtcgga tttggaagat gcgcgccggc gtttgcagac ggctcaagca gacatcgccc
    13441 gtttggaagc ggaaattcct gcagcaatgg cgcaaaaagc ccggcaggct gaagagctga
    13501 ggcaaatcgg agtgcgtttg gactatgctt ggaatgcggt ttacggcgca gggggaacga
    13561 aagcgtcggg gaattgaccg atgaattccc tctttgtgga caatactgtt ttcattacac
    13621 ggctgaaagc cgggcatatc ggcaggttgg ttcaggcgtt gtttgaggag tggcacggat
    13681 ttgaaccgtg gtcttctgtg gataagattc atgcctatta cggcaggtgt ttgaaggatg
    13741 acgaactgcc gctggcattt gcggctgtgg atgatttcgg aaccctgttg ggttcggctg
    13801 cggtcaagcg gcatgatatg gaaagttttc cacagtatga atattggttg ggtgatgtct
    13861 ttgttttacc tgaatatcgc ggaaaaggca ttggcaggag gctggtcgcc cactgcatag
    13921 gcgcagcgcg ttcgctgggg ataaagttct tgtatcttta tacgcctgat gtgcaaatat
    13981 tttatgaatc attcggctgg gcggttgtcg ggcgacattt ccataacggt gaatgggtta
    14041 cggttatgcg tttggatatg gataaggttt aagccgaatg gacaaatgcc gtctgagctg
    14101 ttcggacggc atttttatgt ttggaaactt cttgtcagat aatgtttatc cacagatttt
    14161 tccatatggt ttgatgctgt tccgacaacg gaaattaaga tacggatttg cacaaatctt
    14221 tcagacggta cagttctgac aatgcttcgc gcggggtcag gctgtcggga tcgagttttt
    14281 ccaaggctgc tgccaatata ccttcaaaat gtttttcctc tgctttatcc acaaagttgc
    14341 ccacattcgg ttcatctcct ttttcagacg gcatggtact gaaaatatcc agttggggac
    14401 ggttcgcggc ggcttggttt tccagtccgt tcaaatgctt ttgggcggat ttcaatgcgc
    14461 gtacaggcag gccggcgagt ttggcaacgg cgatgccgta gctttttccg gcgggaccgg
    14521 gttggatttg gtgcagaaac acgatgtcct gtccctgttc gagcgcggaa aggtgcatat
    14581 tgagtgcggc ggcgtgggct tcgggcaggt aggtcagctc gaaatagtgg gtggcaaaca
    14641 ggctgaagga tttgtttttt tgcagcaggt gttcggcaac ggcgtgcgcg agggcgaggc
    14701 cgtcgaaagt ggaagtgcca cgtccgactt cgtccattaa aacaaggctt tgttcggtgg
    14761 cgtgatgcag gatgtaggca gtttcgctca tttcgaccat gaaggtggag cggttggagg
    14821 cgaggtcgtc cgatgcgccg atgcgggtga agatttgatc gatgggcccg attgtggcgg
    14881 catcggcagg cacgaaacag ccggtgtgtg ccaataaaac aatcagcgcg acttggcgca
    14941 tgtaggtgga tttgccgccc atattggggc cggtgagcag catgaggcgg tgtttgtggt
    15001 caaggtcggt gtggttggcg gtgaagtggc gtacctgctg ttcgacaacg ggatggcggc
    15061 cgttttcgat gtggataacc ggatagtcgg caaactcggg gcggacgaag ttccgctctt
    15121 ttgccaaggc tgaaaatgtg gacaatacgt ccagcgcggc ggcggctttg gcggcttttt
    15181 gaagctgcgg caatgccgtc tgaaggtttt tcaatacgcc gtcaaagagt tgtttttcta
    15241 aggcgagggc ttgctcttga gcagtcagta ctttgtcttc aaaggctttc agttccggcg
    15301 tgatgaagcg ttcggcgttt ttgagggttt gccggcgttg gtagtcggca ggtgcttgtt
    15361 cggcttgggt tttggacaat tcaatgtaaa agccgtgaac gcggttgaac tcgactttga
    15421 gtgtggacaa accggtacgt tcgcgttcct tggcttccaa atccagcaaa aattcgtcgc
    15481 catggttttg aatgcggcgc aattcgtcca gttcgggatg aaaaccgtgg ttgatgacat
    15541 tgccgtcttt tagccagacg gaaggttcgg gcaaaatggc ttggcggagc tgttcggctg
    15601 tggataggtt ttccgggaaa acggctttga gggtttccaa caggctgctg ccgtttgtgg
    15661 acaagtcgat ttctgacagg gcaaacaggc tgtcgcgcag ggcggcgagg tcgcgcgggc
    15721 gggcgttgcc gacggcgatg cgggcagcaa tgcgttcaat gtccgcaatg tttttcagac
    15781 ggcattggag gggttcgtat tggctgtcca gcgcggttac agcttcttgg cgggcgcgga
    15841 tgtgggcgcg gttgcgcaaa gggtggtgca gccagagagc caagaggcgg ctgcccatgt
    15901 gggtggtgca aaggtcgagc gtggacatca gggtcggcga ttttttgccg gagagggttt
    15961 gcgtgatttc gagattgcgg cgcgtggcgg catccatacc gatatattgg ctgtcggttt
    16021 cgagcgacag gccgtccaag tgttgcggca tcaggttttg cgtcaggcgg atatagttca
    16081 acagtgcacc tgccgcgcca atcgcaacgg cgtgttcttt gccatccaaa ccgaagccgc
    16141 gcaaatcctg gcagccgaaa tattccgtca gcagtttttc ccccgcgtcg gcggcaaact
    16201 gccacgcgtt caggcgcgta acacccgatg ccgtctgaag ttgcggtgcg tttttactgt
    16261 caggcaacag aatttccgcc gcctgcaggc gcgccagttc gtcgtccaat ttatccacag
    16321 tcgtcagctt ggttttgaat tcgccgcttt gcagcgatgc ccacgccaaa ccgatgtatt
    16381 ttttgtcggg ggacacggca acgatgcggt tggtttcctt gtcttccagc aatgcggaat
    16441 cggttagcgt gccaggcgtt acgatgcgca cgactttgcg ttccaccggc cctttgcctg
    16501 cgccgacttc gccgacctgt tcgcaaatcg ccacgctttt gcccaacttg accaggcgcg
    16561 ccagatattg ttcggcggcg tgaaacggca cgcctgccat tttaatcggt acgccgtcca
    16621 tctgcccgcg cgtggtcagg gtaatatcca aaagttttgc cgcttctacc gcatcgtcga
    16681 aaaacatctc gtaaaaatcg cccatacggt aaaacaccag tttgtcggta tgttgcgctt
    16741 tgatgccgag gtattgctgc atcattgggg aaacggcgga tttggacatg gggctgcctt
    16801 atcattagga aaacggtatt gtagcaaaag gttttgtctt cgacggaatg agactgaccg
    16861 taagcctgtc aataaagttg tggagacaag taggaaaaca gcaaagttat ccacaggaat
    16921 attattaagt caaaaaacat ccacgtttat ctgtcctata cggcaagcct taaactcaaa
    16981 cttatttgac gaaaaggtaa tgaaaatatt ggatatttgc aacttataca caagcagatc
    17041 cgcaatcatc aaaatcatca ttaaaatttt aaattcaaat attgatggta aaagttttgg
    17101 aaatctgtgc aaacagtaaa attcatctat atcaaataag catcaagtag ttagatgtaa
    17161 tagtggaatg taactttatt tcagaataca atacagatat tgaaatttcg tttcacagct
    17221 tggagtatat acatgagcat taaagtagcg attaacggtt tcggccgcat cggccgcctc
    17281 gcattgcgtc aaattgaaaa agcccacggc atcgaagtcg tcgctgtcaa cgacctgacc
    17341 cctgccgaaa tgctgctgca ccttttcaaa tacgacagca cgcaaggccg tttccaaggt
    17401 actgccgaat taaaagacga tgccatcgtg gtaaacggca aagaaatcaa agttttcgcc
    17461 aatccgaatc ccgaagaatt gccttggggc gagttgggtg tggatgtcgt cctcgaatgt
    17521 accggcttct ttaccaataa aaccaaagcc gaagcacaca tcagggccgg cgcgcgcaaa
    17581 gtcgtgattt ccgcacccag cggcaatgat gtgaaaaccg tcgtatatgg cgtgaaccaa
    17641 gacattttgg acggcagcga aaccgttatc tccgctgctt cctgcaccac caactgcctt
    17701 gccccgatgg cggcagtcct gcaaaaggaa ttcggcgtag tcgaaggcct gatgaccacc
    17761 atccacgcct acaccagcga ccaaaacacc cttgacgcgc cgcaccgcaa aggcgatttg
    17821 cgccgcgccc gcgccgccgc gctcaacatc gtgcccaaca gcaccggcgc cgccaaggcc
    17881 atcggtctgg ttatccctga attgaacggc aaactcgacg gctccgccca acgcgtccct
    17941 gttgccaccg gctcgctgac cgaattggtt tccgtcctcg aacgccctgt aaccaaagaa
    18001 gaaatcaacg ccgcgatgaa agcagccgcc agcgaatctt acggctacaa cgaagatcaa
    18061 atcgtttctt ccgacgttgt cggcatcgaa tacggctcgc tcttcgatgc gacccaaacc
    18121 cgcgtgatga ccgtgggcgg caaacaattg gtgaaaaccg ttgcctggta cgacaacgaa
    18181 atgtcctaca cctgccaact cgtccgcact ttggaatact ttgcaggcaa aatctaaggc
    18241 ttgaacaaac ctgtggataa gtaaatgccg tctgaacaca ttgcgttcag acggcattgt
    18301 ttatctgtat agtggattaa atttaaacca gtacggcgtt acctcgcctt gccgtactat
    18361 ttgtactgtc tgcggcttcg ttgccttgtc ctgattta
//
LOCUS       DAWHXS010000038        17834 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWHXS010000038 DAWHXS010000000
VERSION     DAWHXS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.038"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(233..1426)
                     /locus_tag="WIE22_000972"
     CDS             complement(233..1426)
                     /locus_tag="WIE22_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ4127075.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKIRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQIQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1423..2406)
                     /locus_tag="WIE22_000973"
     CDS             complement(1423..2406)
                     /locus_tag="WIE22_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ4127076.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2417..3853)
                     /locus_tag="WIE22_000974"
     CDS             complement(2417..3853)
                     /locus_tag="WIE22_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ4127077.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANASGWNAPQQADMLLLASFASVMLLYPYLQRAFDGVVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(3981..4136)
                     /locus_tag="WIE22_000975"
                     /pseudo
     CDS             complement(3981..4136)
                     /locus_tag="WIE22_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            4488..4700
                     /locus_tag="WIE22_000976"
     CDS             4488..4700
                     /locus_tag="WIE22_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ4127078.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            4848..6431
                     /locus_tag="WIE22_000977"
     CDS             4848..6431
                     /locus_tag="WIE22_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ4127079.1"
                     /translation="MSSKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIERMGLENVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDTVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLRDIMQFSSRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLDRRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            6513..7610
                     /locus_tag="WIE22_000978"
     CDS             6513..7610
                     /locus_tag="WIE22_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ4127080.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRVLTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVRVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASESVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKVGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIAVVQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            7724..8839
                     /locus_tag="WIE22_000979"
     CDS             7724..8839
                     /locus_tag="WIE22_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ4127081.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNLASVSTENRYKIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     VVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            8964..9419
                     /locus_tag="WIE22_000980"
     CDS             8964..9419
                     /locus_tag="WIE22_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ4127082.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            9490..9966
                     /locus_tag="WIE22_000981"
     CDS             9490..9966
                     /locus_tag="WIE22_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ4127083.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            9954..10880
                     /locus_tag="WIE22_000982"
     CDS             9954..10880
                     /locus_tag="WIE22_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ4127084.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMEQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAASKTD
                     LCTPEQLVEFHDKAAKLFPPKAKVVEVRNAQLDIQWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            10945..11499
                     /locus_tag="WIE22_000983"
     CDS             10945..11499
                     /locus_tag="WIE22_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4127085.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVE"
     gene            complement(11578..11682)
                     /locus_tag="WIE22_000984"
                     /pseudo
     CDS             complement(11578..11682)
                     /locus_tag="WIE22_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11738..12697)
                     /gene="ttcA"
                     /locus_tag="WIE22_000985"
     CDS             complement(11738..12697)
                     /gene="ttcA"
                     /locus_tag="WIE22_000985"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ4127086.1"
                     /translation="MSKKIKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(12790..13653)
                     /locus_tag="WIE22_000986"
     CDS             complement(12790..13653)
                     /locus_tag="WIE22_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ4127087.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKVVFEILEDYAVGAEL
                     EASAEPLAAQEPSLVFTAECVSDGICSVVLPHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTENAE"
     gene            complement(13688..14050)
                     /locus_tag="WIE22_000987"
     CDS             complement(13688..14050)
                     /locus_tag="WIE22_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ4127088.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            14125..14604
                     /gene="ybaK"
                     /locus_tag="WIE22_000988"
     CDS             14125..14604
                     /gene="ybaK"
                     /locus_tag="WIE22_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ4127089.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGNGLIVLMHGDKQISTRNLARHLGAKRIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            14626..15348
                     /locus_tag="WIE22_000989"
     CDS             14626..15348
                     /locus_tag="WIE22_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ4127090.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            15350..16225
                     /locus_tag="WIE22_000990"
     CDS             15350..16225
                     /locus_tag="WIE22_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4127091.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(16297..17694)
                     /gene="aspA"
                     /locus_tag="WIE22_000991"
     CDS             complement(16297..17694)
                     /gene="aspA"
                     /locus_tag="WIE22_000991"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ4127092.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         4129 a         4390 c         5107 g         4208 t
ORIGIN      
        1 cgtctgaaaa cggaaggcgg cagtttatca tctttgcctt cagacggcat atcaaacgcc
       61 gcctttgcgc cgtatgccgg aggttcgccc ccgtccttcc gatgcttgag gcgaatgccg
      121 gttgcgtccg gcaaaccgtg cgcggggtgg ctatgccgtc tgaagcaata tgaaggtatg
      181 cgtttcagac ggcatccccc tgttgcggaa ataccgtccg cctcctgtcc gattattgcc
      241 tgattgcagc ggcgcggacg agttcttcga ggctgtcggc gagcatttcc gccgcgcgcc
      301 ggtagccttt ggcggagaag tgtacgccgt ctttggcggc ccatccttgg ttgagccagt
      361 ttttcatgct gcatatgccg cccatggcgt tttgccaaga ccagaacatg gtttgtccct
      421 gccgggcgac gcgccgctgt atctgttgga cttcggtcag gcggacgggg cgtgtgccgc
      481 atacgccgag cgtgtttttc agggattcgg gtgcgccgat gatgaggatg ccggcggcag
      541 gcaggctgtc gcggatttgg cggacggtat ccagccattt ttgttcggtg tcggcaatgt
      601 cgatgttgtc gccgaaggct tcgttggtgc cgtaggaaag gataaccaaa tcggcgccgg
      661 tttgggcaag gtcgttcata cggtcggcac gccatttcga ccaatgggtc aattgtgcgc
      721 cgttgatgcc catggcggaa acggtaatgc cgccggcggg attttcgatg ttgatgaagc
      781 cgatgtccca cggcatttcg gtctgtatgg tcaagggcag tgccgcgcgg gtatccagta
      841 cctgccagcc gccgccgttg gcggagacgg tgttgccgtt gacggtcagg gtttgttcgg
      901 cgagcagggg tttggcaaac agggaaatgc gctgtttgcc ggtcttgccg tccgatgcgg
      961 tcagggtcat gctgccgccg ctgccggtgt gggcgaggat gccgccgagc gggaagtctc
     1021 cgctattgtt cctgctggtg aggctttgcc agttgccgtt gtgccggact gccgccatgc
     1081 gctgcccttt gacgttggcg gggtaaaccc agcctatgcc gccgtcgccc caagtttttt
     1141 gcaggcgttt gcgcaggctg tcggtaaaga agtcgccggc ggtatgcgag tcgccgattt
     1201 gcaggatgcg gaaggtctcg ccgctgcctt gtgcgacgga tcggattttt ttcatccaag
     1261 gcgaggcggc ggcgttgccg tagtcggtca agagcccgtt tgtatcggtg tagggggcgg
     1321 acggggaaac ggtaacggtg tcgaggctga cggaggcgac gggcagggct tctgcctgcg
     1381 tggcggcgaa cagggcggaa aatgcgatga ggtgtttggg gttcatggct gtgtacttga
     1441 tggttgcgta cttggttcaa aaacgatttt ttccattatt tttgccgcca gcagtttttg
     1501 tccttcggcg gtaaagtgta tgccgtcctt gctgcggtag cggacgggtt tgccgttgac
     1561 gttgacggag tcggtgtagc ggtcttcccc gccgctcagg gtgtgcgcgg tgggaatcag
     1621 gatgattttg ccttccaggt gttccgaaag cagtttgtcg aggtagcgca tctgcccgtc
     1681 gagcttggct tttttcatat aggggatgcc gagccagacg acttggacgc ggtgcgtgtg
     1741 tgcggcttca aggatgcggt cgacgcgttt caggtattct tgcgcccatt cgtcggaagc
     1801 gaatttgagg tatcgtttgc cgacggggaa atcccacggg tcgttcgggc cgaggaagac
     1861 ggcgagcacg ctgatttcgg gatgtttttt cagggtttct tcaatcgttt tcggccagtc
     1921 gaagaatgag gggtaggaca gccccgtgct ttgtttgctg aggttgacgg attcgatgcc
     1981 gtattgctgt ttcaggcttt tttgcacgaa gggtgcaacg ccctgcatca gcgagtcgcc
     2041 ggcaaaaaag actttgtcgc cgctgcggac ggcggcggct tcggtgtctt gtttccattc
     2101 tgtttcaccg gtttgaggga cggcttctga tgcggcggct tcagacggca tatctgccga
     2161 accgccgtct tgagccgtcg gcggcgtttc gccggacagg aaggctttga tgccgtctga
     2221 aagggcgtag gcgttttctt gcaacgccac accgctccgc caccatccgt aggcggcgag
     2281 cggttcgagc gggctgttgc ggtggtaggt ctgctgccag taggcgttga tggggttttg
     2341 gctgaaccac acggcaatca gggcagacat caatatggag gcgaaaaggg aaagaaagtt
     2401 tttcatgtcc aaacccttaa aaattggcat aaataaagcc gggtatcccc gagggggcga
     2461 ggacgataat cagcagcagg acggcggaaa cggggataaa ccacagccac atcgggattt
     2521 tttccaaacc tttgaccacg ccgtcgaaag cgcgttgcag gtaagggtag agcagcatca
     2581 cggatgcaaa cgaggcaagc agcagcatgt ctgcctgttg cggcgcattc cagccgctgg
     2641 cattggcaaa gagcgcgctg aaaaccgcgc cggcatcgtc ggggtttgcg gtattgaaga
     2701 cgacaaagct aaggcagaca aaatggaagg taacaaacca cgagagcggc gcaaggtatt
     2761 tcagacggca tagcgcgtcg cgtccgaaat aacggtcgcc cgtgttgagc agcaccagcg
     2821 ccgtgccgtg cagcgcgccc caaatgagga agttccagcc gtagccgtgc catatgccgg
     2881 agagtaccat tgccgccatc aggttgagct gtgtccgtaa aaagcctttt ttgctgccgc
     2941 ccaaggggat gtagatgtag tcgcgtatcc aggtggaaag gctgatgtgc catttgtccc
     3001 aaaatgcgcg gatgtttaaa gcacgaagcg gtgcggagaa gtttttgggc agccggaaac
     3061 ccagcagcat cgccatgccg ataaccaaat cggaatatcc ggaaaagtct aaaaagagtt
     3121 ggaaggtata gccgtacacg ccgcccaata cgccccagcc gtcgaattgg gcgggatttt
     3181 caaatacggg cgacacccag ttttccgcca gcatccccgc cagccaccat tttttggcaa
     3241 tacccagcag aatcagggaa acggcgagtg cggggcggac gggtgagcgc ggttggcggg
     3301 tacggatttg cgccaatgcg cccgcctgct cgccgtccgc gcttttgaat gcggcggcgc
     3361 ggataatcgg gccagaggta acggtgggaa agaaactcag gtgtagtagc agctcgtgcc
     3421 agctgaaacg cgcagcgtgc ggggcgcgga agcagtaaac cagataggcg agcgactgga
     3481 aggtgtaata cgaaagcccc agcggcatca ggatgtcgat tgcgccgcct tttccggcat
     3541 attgggcaat cagcgggcgg aaaaagtcga aatatttgaa aaagcccaag acggtcagcg
     3601 agacggcaat gccgcacccc agccaaaagc ggcgcgtatt ttcgcgatcg gaacggagca
     3661 gttcgcccaa aaggtacacg caggaggaat aaaggacgac gattgccgca aatacggggc
     3721 cgatatggta gagccagccc ataccggcag ccaaaagcag caggttttgg acggacgggt
     3781 atttcgccaa gccccagtaa atcggcagga aggcgagaaa gaacagtgcg aactcgacag
     3841 acagcagcgg cataaaccat ccttggaaca aaaaataggc gttaaaacac ggcaaagccc
     3901 gttattttaa gcaggggtgg atatttcttc aatcaaaatg ccgtctgaag ggcgggagag
     3961 gcttcagacg gcatcgcggg tcaggttcgg atatggcggt tcaagttcag gcacagccac
     4021 agccacagcc cggccgccgc cgcaagcata ccggcgatgc cgatacggga aataccgaca
     4081 tattgcgtta cccaatgtcc caacagtgcg ccgctgccga taccgacgtt gtatagtgga
     4141 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggaaccgat
     4201 tcacttggta cttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaacgac
     4261 gtactggttt ttgttaatcc actataaatg cgccgattgc caaggcgatg atgctcaacc
     4321 agggtttttg cgaagcgttc attgttgtcg tcctgatggg aattgccgcc attatgcgca
     4381 agcgtccgtc tttttccaaa ccgccgcacc gtttccgcgt ttgcccaagt tcacgccccg
     4441 acttacaata acgcccgtca gattgcagtt ccaacgaaag gaaaatgatg gaaaccctta
     4501 tcctcgacat cggcggcatg agctgcggcg gctgcgtcaa aagcgttacc cggatattgg
     4561 aaggcgtaaa aggcgtggca agcgtcgaag tcagccttga aaacaaaagt gcgaccgtcg
     4621 gatacgaccc cgcgcaaacc gatgcagggg cgttgattga agccgttgaa gacggcggct
     4681 atgatgccgc gttgaaataa agcggcaaaa atgccgtctg aagccttcgc gcctcctaga
     4741 tgccgtctga agtacgctcc gcgccttcag atggcatttg atttagttga acgggtagga
     4801 tgaattggtt aaacttgcgg gcatattgtc tttagagtga acaaaatatg tcgagcaaga
     4861 agaaaaaagc cgcttttgcc cgtgctgcaa cggagaacgg tgtgccggac gtgtccaaca
     4921 tcaaaaattc atatgatgat ttgatgtacg aatccggcgc attctctcaa accgccatca
     4981 ataatttgga ggcgcgcgcc cgcctgatgg gcttgcagcc cgcgcccgcc gccaatgcca
     5041 aggtcttgga gctgggctgt tcgatgggcg gcaacatcat cacgcaggcg ctttattacc
     5101 cggatgcgga atttgtcggt atcgacttgt ccggcaggca ggttgcgcag ggcaacgcca
     5161 tcattgaaag aatgggcttg gaaaatgtgc gtctggaaga aaaagatatt ttgaccatcg
     5221 atgagtcatt cgggaagttc gactatatca tcgtccacgg tatttggtcg tgggttcccg
     5281 atacggttaa agacaaaatc ttttcgattt gccggaacaa cctgacggaa cacggcattg
     5341 cctatatttc gtacaatgtc taccccggct ggaaacggca ggagcagttg cgcgatatta
     5401 tgcagttttc cagcagggat gtgcttgaag aacccttgga agcgcgaacg cggaaaggct
     5461 tggacgcgct caaggcactg gcggaaattt tggaaaacga caagggcttg ggcggcggcg
     5521 gcaaacttcc ggcgattcaa aaaatattga atcataattt ttattacatc gcacacgaat
     5581 atatggaggc gtttaacgac ccgatttacg tcaacggctt catcgaatgg gctaaccgcc
     5641 acaggctcgc atatatcggg gataccaatt tgcacgtgtc ttttgtttct tggatggcgg
     5701 agcatacgcg ggagcggatt ctggcattgg caggggacga ttatattgcc aaagaatttt
     5761 acagtgatat tttatccgac cgccaattcc gccgttcgct tttatgccgt gaggaagtcg
     5821 gggatactgt caggcgtgat gagtcggttg ccgtcgaagt gatagaaagt ttgaatttcc
     5881 gcccggcaag aggggaaaca atcaactttg atgaaaacga tatcctgctt tcgggcatac
     5941 gcgatgtgat gaaaaccgga gaggcgttta aaacggaaga tgttgcggaa aatctcgacc
     6001 gccgatttcc cggtctggaa tttgaccgca tgaaaatcaa ttcccagctg ttattgcaaa
     6061 ccattctcgg gcgtttttct gtttcatcag acaatgcggg caaaccattt tttgaagacc
     6121 ataaaaccta tgtgccggcg cgttttacag attatgccgc cgcctttgtg gaacatggcg
     6181 cggaagcgtt tgtccggcct gccaaccgtt acaacgaaag caccccctca ttcggatacg
     6241 ggcatttgta cattatgcgg caattgtcgc ggccgacgag caaacaggca ttgattgaaa
     6301 cggttgccga aaacttgaac atcgtcagca ccacgcccga cggtttgaca ttccatccgc
     6361 ctgccgaagt gtatgtggaa gaaatattgg cagacttggc agacaggcat tttctcgttt
     6421 cggcggattg acgggcaagt cccggcggca atccgtccga tatattttca aacaggaaaa
     6481 acacacactg cgaaaagcgg tacacgacat atatgaacca gacatttacc ttgcccgata
     6541 cgcgcccgta tccccaaaat ccgattaaga accacctgct gctcaatgcc taccagttgg
     6601 cgcataattc ttcccaggct tcgcgcaaac tctcgtccgg ccaacttcaa accgaaatca
     6661 gggggatgct tgagcaaaac cactatatca acctttccct cgcgttgacg atgtcgcccg
     6721 atgccggaac ttatgccgcg ctgctttcca gtgtgaacgc ggtactcgat tgcgagaaag
     6781 aaggcgaagt gcagtggttc gccctgccgg tcgtgctggt gtccggctgc aagaaagaac
     6841 gggcaatcga gatgaagctg ccgacggagg cattgtttgc ctgcctgcaa aactatccgc
     6901 acctgcgcgt tttgacgcaa gagacgcaat ggctgcctta tcttgtgcat tcttccgatt
     6961 tgagcgcggt cgcgccggat gagtggtggc gtgccaaaca aaataccgaa gcggcggcgc
     7021 aacacttgcg ccgtttcgcc ccgcgccctt tgctgttgcc cgaagggcag tccgtccgcg
     7081 tcgtttacgc gctgggtttc ggcagcggca aggttcagac ggcattgggt cagaaccttc
     7141 ttcaggcagg cctgccctta atgcaggtat ggcaggaaaa tcttgcatcg gaaagcgtta
     7201 cgctgtttgc caatccgctt tccccggatt ctccggtacg cgcgctttca gacggcagcc
     7261 acacgcgcca gcgtatggcg atggatgtgt ttgcggcaaa cgcgatacgc gccgtccgta
     7321 tgcagagtcc gcgcgtcggc gtggtcgctg cggcaaaggt gggcgggcag gttttattcg
     7381 ggtttaatgc gaccgacggc gcgtttgaag tcgtgccgca ggtgttttcg tggcagcttt
     7441 cctttaccga caatatcgcc gttgttcagc aaaatttttt ggatttaatg gcagaatgcc
     7501 gtgtagaaca cgtttacctg ctgcacaatc ccttgggcga acaggaaagc atcccgagct
     7561 atgcggaagc attgaaacgg gaagggcaca atccgttttt cagcgcatag tgatttgtag
     7621 tagtgatagg ctttctcatt tatataataa atcaaaataa agagcacaac actttttcat
     7681 tctgtgttgt gccttgagtg aaacgaaagg atgaattata aatatgaaga ttgtaattgc
     7741 gccggattcg tttaaggaaa gcttgacagc tcaacaggta gctgaagcaa taaaaagagg
     7801 cttccaacaa tcgatagcag atgtggaatg tctcctctgt cctgtcggtg atgggggaga
     7861 aggcactgta gatgctatcc gacattctct tgacctagaa gaaaaatgtc tccaagtgac
     7921 aggacctttt ggacaaaaag aagtcatgcg ctattttcaa aaagaacaac tagccctatt
     7981 cgaagttgct gatttggttg gtcttggaaa aatcccgcta gagaaacgaa atccattaca
     8041 aatccaaact cgtggtattg gagaattgat tcgccacctc attagtcaag aaattaaaga
     8101 aatctatatt ggcgttggcg gtacggccag taatgatgga ggtattggga ttgctgctgg
     8161 tttaggttat caattttatg atgaggatgg aaatgcctta cccgcttgcg gtcaatcctt
     8221 actaaaccta gcttctgttt caacagaaaa tcgctataaa attcctgaag atgttcacat
     8281 tcgtatttta gcagatgttg tgagtccctt atgtggacac caaggtgcga cctatacgtt
     8341 tggcaagcaa aaagggttgg attctactat gtttgaggtc gtagatcagg caatacaaga
     8401 tttttatgaa aaagtctccc ctgcaacatt aaaacttaaa ggagcaggag ctggtggagg
     8461 catcgctggt ggtttgtgcg cctttgctca ggcaagtatc gtatctggaa ttgacacctg
     8521 cctggacttg attgactttg ataagaaagt gtcagatgtt gacttggtta tcgttggtga
     8581 aggaagacta gatcgtcaaa gtttagcagg gaaagcgcct attggtgtag caaaaagaac
     8641 ccctgtcgga gttcctgttg tcgctatttg tggcagtctc gttgaagatt tgccttccct
     8701 gccatttgaa aatatccaag ctgccttttc tattttggag aaaagtgaac ctttagaaga
     8761 tagtttgaaa aatgccagtc tctatttgga gcatacggct tctaatattg gacacttatt
     8821 aaatatgcct aagatttagc caaaccattt cttccagatg gatgttttgg ctggttctgc
     8881 cttatgcggt tacgattaac cgaccgatga cgtctgaaag ggtttccgct tcagacggca
     8941 tcgcgccgga acagggaggg gatatgaaaa aaccgaaaat cctttttgtc tgcctcggca
     9001 acatctgccg ttcgccgatg gcggaataca ttttgcgccg ccgcgccgcc gaagcgggca
     9061 ttccccttga agcggacagc gcggggacat cgggctggca cgacggcgaa gatatgcacc
     9121 gcgagacggc aaagatattg aaaaaacacg gtatcgatgc ttcaggcttt accagccgca
     9181 aaatccgcca aagcgatgcg gcggcgtttg actgcatcat cgcgatggac ggcaagaatt
     9241 tgtccgaatt ggaaagaacc ttcggcaggc ggtcggaaaa aatattcaag ctgaccgacc
     9301 tgatacccga aagcggttac gaccatgtcc ccgatccgtg gtacacgggc gattttgaag
     9361 aaaccttcag gcttgcggat gcgggctgtc gggcattgtt ggaaaagatt tccaaataaa
     9421 gcaattgaat acaaatataa aaccgcctcc tgccgtatcg gttgttcaga cggcataaac
     9481 agagaattta tgaaaacaaa tttcaaacag aaaattattg aacaggcacg cagcgaaggc
     9541 ttgcaggtaa ccgctttgcg cgagcaggta ttagatattg tcttgcagca aagcggcgtg
     9601 attaaagcct acaacgtctt gtcgcagatg cagcagcaaa gcgagggcgt gcttgcaccg
     9661 cctaccgcct accgcgccct tgatttttgg gcggagcagg gcgttttgca caaagtggcg
     9721 gcggtcaacg gctatatttt gtgcagccac gcgcagcacg agtgcgacga ccattgccac
     9781 gaccacgaag aagccgaagc gcaccacagc gcgtttattt tggtctgcac cgaatgtggc
     9841 acggcggacg agcaaaccct cagccacgag tgggcggcac tgcgcgcagg cgttgccgaa
     9901 agcggctttg cgctgaaaga agaacacgtt gttttaaccg ggatttgtaa aaaatgtcag
     9961 aaatgaaaaa aaccaaagtc cacctgattt caggttttct gggaacaggc aaaaccaccg
    10021 cgctcaaaag tctgatggaa caaaaagacc ccaacgaaaa atgggtcatc atcgtcaacg
    10081 agtttggcga aatcggcatt gacggcgcgg tattgagcga caacggcatc cctgtggcag
    10141 aaatcgccgg cggctgtttg tgttgcaccg ccggcccgca aatgggcgta accgtgcaga
    10201 aaatgctgcg cgatgccaag cccgaccgcc tgatgattga ggcaagcgga ctggcgcacg
    10261 ccgccagcgt catcgacgaa ctcaaagcca aaccgctgga cagccttttg gaaatcggcg
    10321 cggtctttac cgtcgtcgat ccgcgccaat tcatcaaccc cgattacgcg caacaggcat
    10381 tgtataaaga ccaaatcggc atttgcgacg tattggccgc cagcaaaacc gatttatgca
    10441 cccccgaaca gctggtcgaa tttcacgaca aagcggcaaa actgttcccg cccaaagcca
    10501 aagtggtcga agtccgaaac gcgcaactcg atatccaatg gctcgatatt cccgtcatcg
    10561 aaaaatcacg ctaccgcctc aaagccctgc cggacaacac gatgggcttc cagtcgcaag
    10621 gtttcacatt ccccgccgga cgcgatttcg acggcgaaaa attgaccaac ttcttcaatg
    10681 atttgcccaa gatgaccgaa ggactcgtcc gcgccaaagg cgtgtttcaa gtactcggca
    10741 cttgggtgtg gctcaactgg gtggacgggc aatggggtgc gaaccaagtc tcgtggcggc
    10801 gcgattcgcg ttttgaattg attgccaaat cgtttgatgc ggatttaatc gaacaaaagc
    10861 tcaaagacgc attggaatag cgttcacgcg ccttcagacg gcatatgaaa tgaaaatgcc
    10921 gtctgaaggt ctgaggagga cgggatgaaa cccaaattca aaaccgtttt aaccgcgctg
    10981 cttttggcgg tttccctgcc gtctatggcg gcaacccatg ttttgatgga aaccgatatg
    11041 ggcaatatcc gtttggtttt ggacgaatcc aaagcaccca aaaccgttgc caatttcgtg
    11101 cgctatgccc gaaaaggctt ttacgacaat acgatttttc accgcgtcat cggcggcttc
    11161 gttatccaag gcggcggatt gaccgaggac ttggcacaaa aggcaagcga taaggccgtt
    11221 gccaacgaat ccggcaacgg cttgaaaaac actgtcggca ccatcgccat ggcgcggacg
    11281 acagaccccg attccgccac cagccaattc tttatcaatc tggcggacaa tgattcactc
    11341 aactacaaaa acggacaata cggctatacc gttttcggca gggtcgaaag cggaatggac
    11401 accgtttcca aaatcgcccg cgtcaaaacc gccacgcgcg gcttttatca aaacgtaccc
    11461 gtacagcccg tcaaaatccg tcgcgttgtt gtcgagtaat ataacgggaa accttcagaa
    11521 agctgaaacg gaaaaagcag gtgtttgggg ttgaaaagcg tctgcttcgg gattttataa
    11581 tggattaaat ttaaaccagt acggtgttgt ctcgccttgc cgtactattt gtactgtctg
    11641 cggcttcgtc gccttgtcct aatttaaatt taatccacta taaaatgccg tctgaaacgc
    11701 caaacgggct tcagacggca tttttgacgg cggggggtta cgcgccgcag gttttcggct
    11761 tgttcgccag aatgttgatg actttgcgtt cggctttttg cggctcgatt ttgatttcgc
    11821 tttcgtcttc ttcgctgccg tctgaaaaac gttcgggcat tttttcgctg tcaaacgcca
    11881 aatcgccgcc gtgtttcagg ctttgaccgc gttccaatcc gacaaagtcg aagagttcgg
    11941 tatcggcaag gtgggaaggg acgacgtttt gcagggcgga gaacatcgat tcgatgcggc
    12001 cggggaagcg tttgtcccaa tcgcgcagca tatcgccgat gacttggcgt tgcaggttgg
    12061 gttgcgagcc gcagaggttg cacgggatga tggggaattg ttttaattcg gcgtatttga
    12121 ttaagtcttt ttcttttaca tacgccagcg gacggatgac gatgtgttcg ccgttgtcgc
    12181 tcaccagctt gggcggcatg gctttgagtt tgccgccgta aaacatattt aaaaacaagg
    12241 tggcgaggat gtcgtcgcgg tggtgtccca aggcgatttt ggtgcagccc aattcttttg
    12301 cagtgcggta gaggatgccg cggcgcaggc ggctgcacag cgaacaagtc gttttgcctt
    12361 cgtctaatac gcgtttgacg gtggagtagg tgtcttcttc aacgattttg tagggaacgc
    12421 cgatgctttc gagataggtc ggcaatactt cttcggggaa gcccggctgc ttttggtcga
    12481 gattgacggc aactagttgg aaatcaatcg gcgcgctggc ttggagccgg cgcaggatgt
    12541 ccaagagggc atagctgtct tttccgccgg agaggcagac catgattttg tcgcccggct
    12601 cgatcatatt gaaatcgtta atcgcgtcgc cgacggcgtg gcgcaggcgt ttgctgagtt
    12661 tgttgttttc gagttcttgc ttgatttttt tggacataac ggttcgggtt tgaaaattgg
    12721 aaaggcggca ttgtaaccga ttggcggggc ggcaatgccg tctgaagggc ttcagacggc
    12781 atcggcggct tattctgcat tttcggtttt aaagaagaga tgaaccgctt tgaagatacc
    12841 gccgtttggg acggtcagtt ctttttgtgc ggcggagaat ttaatcacgg taagggcggt
    12901 aaagttttcc gaatcttgaa cgctgtcgag tacgatgccg gcctcttcgc cgtccgccgt
    12961 cagcagggtt cctgcttcga cggccgaatt tcccgacaat accgccaagc cgcgtttgac
    13021 ctgcccccga tactgggcac gggcaatgat ttcctgtccc ggatagcagc cttttttgaa
    13081 gtgtacgccg ccgatgatgt gctggttgag catttgggcg acggcggttt ctttggtagc
    13141 cgcgcatatc cacgggtaac cgctgcggat ttcgtgcagc cgccacgcgt tttcggcggc
    13201 ggcatcataa ggcggcaggg cggtttcggg ggcgatgtgc aaaatgcccc gatggggcag
    13261 gacgacggaa cagatgccgt ctgaaacgca ttcggcggta aagacgagac tgggttcttg
    13321 cgcggcaagc ggttcggcgg atgcttctaa ttccgcgccg acggcgtaat cttcaaggat
    13381 ttcaaaaacg actttggcac gcaacacaaa catccgcagg cgtttgacgg ttgcttcaag
    13441 caggtcttgc gccataatca gcagcaaatc gccgcctcgg ttgacgacaa tcatattggc
    13501 gatgacgcgg cctttgggcg tgttgtaagt cgcataacac gcctgcccgg tctgaagatt
    13561 attgatgtcg ttggaaagct gtccgtgcag gaaggtttgg cggtcttcgc cactgacgcg
    13621 caccacgccg aaaaagggca gtaaggtttt catcatttgc ctactctgaa aatataagga
    13681 aatctgttta tgcagttgcc gcgtctctct tcacggcggt tattttgatt tcgacgcgcc
    13741 actccggacg ggcgagccgc gcttccacgc aggcgcgggc gggcgttctg ccggcggcaa
    13801 cccaagcgtc ccacacgccg ttcatttccg catagtcgcc catatcgcgc agatagatga
    13861 ccgcatccaa aacgtgcgct ttgtccgagc cgcattccgc cagccagcgg tcgatttggg
    13921 caagcacgtc ggcagtctgt tcggcagccg tttcaccgtt ttcgggaacc atgccagaga
    13981 ggaaaatcaa gccgtttgcg ccgacggctt cggaatagcg gggcgttgtg ccgaaatatc
    14041 ggatatccat atcggtttcc ttcgataaag gggatatatg gtaacattgc gcttgaccga
    14101 tttccatgtt ttgcatgacg aaaaatgagt aaacacactt atccgataac acctgccgtg
    14161 cgcgttttgc gtgaaaacgg catcgaattt gaacctttta cctatgccta tgaggaacac
    14221 ggcggcacgg cgcagtttgc ccgactattc ggcaaagacg aacacttggt cattaaaacc
    14281 attgttttgc aagatgaaaa cgggaatggg ctgattgtct tgatgcacgg cgacaaacag
    14341 atttccacgc gcaatctggc tcggcatttg ggtgcgaaac gcatcgaacc cgctacgccc
    14401 gcacaggcaa acaagtggac gggctatctg gtcggcggca caacgccgtt cggcatccgg
    14461 acaaaattgg atatttacgt cgaacagtcg gtgatggatt tggaaaccat ctatatcaac
    14521 ggcgggaaac gcgggttcat tatcggtatc cgtcccggag atttaaatat tttgaacccg
    14581 aaaacaatac aggcggcggt ttgaccggga agtataagga acaatatgga caaagatttg
    14641 tatgccgtat tgggcgtgtc gccgcaggcg ggggcggacg aaatcaaacg cgcctaccgc
    14701 aagctggcaa tgaaatacca tcccgaccgc aatccgggca atccgcaggc ggaagaaaag
    14761 ttcaaagaaa tccaacgggc ttacgatacg ctttccgacc tgtcgaaacg gatgcaatac
    14821 gacgcgtcct tcagacggca tgaggaacgc gggcggcagg aagaggcatt ccgccgcgaa
    14881 caggcgcgca gggagcagtt ttaccgcgaa cagatgcgcc gcgaacaggc gttcagacag
    14941 gcgtttgaac ggcaggcatc acgttcgtgc catacttacg aaccgtccgg cggcggaagc
    15001 gggcgcaact atgtcctcgc cgcctacatc ctgttcggtt tgggtgcaat catgctgttc
    15061 atgcccatag tcggcgtgat tttcgcctat atgcccatag tcggcgtgat tctcgcctat
    15121 atgaaacgga acagtttgga cagcattgtc tatgccgcac ataccgaata cctgattaaa
    15181 accttttggc gcacattttg gctttatatt ttgggtgcgc tgactgccct tttgggtatc
    15241 ggcgtgctga ttattattgc aacgaacgtc tggtatttct accgcatcat cgccggcttt
    15301 atccgcttca acggcggcag ggcggttgca cccgagaaat ggatatagta tggcttacct
    15361 gttaatcagc atcgtgttca gcgtgtcggt ttccattttg ctgaaaatgg caaggaagaa
    15421 aaaaatcgac atcgcgcagg cggtcgccgt caattatgtg gtcgcggtca tactgaccct
    15481 gctggtattg aagccggatg tcggcaatat cggcgcattt ttgccgacat ggtggctgtt
    15541 tgccgcattg ggcgtgctgc tgccgtccgt atttgtcatt atgggcaaat ccgtagaatc
    15601 cgccggtatc gtcaaatccg acgcggcgca gcgtttgtcg ctgtttttgc cgattgttgc
    15661 cgccttgacg ctgtttggcg aaaaactcag cgaaggcaga ctaatcgggc tgtgcctcgc
    15721 atttgccgca ctgttctgcc tgctttggaa acacagcggt ggcaaaaaat caggaagcgc
    15781 gtggcggcag gcggcattgc tgctgggcgt gtgggcaggt tacggcatta tcgatatcct
    15841 gttcaaacag cttgccaaaa gcggaacggc atttgcgggc aacctgctgg ttgcatttgt
    15901 gctggcgggt gtgctgatgt ttgcctgcct gtttgccaaa tcggtcagat ggcgtgttga
    15961 gagtgtggtc ggcggcatat tcttgggcgg tttgaatttt atgaatatcg taacctacat
    16021 caccgcgcac caaatgatga aggataatcc gaccttggtt tttgccggta tgaatatcgg
    16081 cgtgattgtt ttgggtacgc tttcgggcgc attgttcttt aaggaaaaaa tcaacacaat
    16141 caatacggcg ggaatcgtgt tggcactgtg ttctatcgcc tgcctgtttt attgggggga
    16201 agtcaaggta ctgttcggca tataatccgg ctgattgata taacaaaatg ccgtctgaag
    16261 cagcatccct gcttcagacg gcatttgtct gcaacgttac agatgggggt tcatcaggtt
    16321 ctcgggagag aggatgcggt tgagttcttc ttcgctcaac agcccgcgtt ccaagacaac
    16381 ctcgcgcacg cctttgccgg tttgggcgca gattttgccg accaaatcgc cgttgtggtg
    16441 tccgatatac ggattcagat aagtcaccaa accgatggag ttgaaaacgt aacgttcgca
    16501 gatttcgcgg ttgaccgtaa tgcctttgac gcatttgtcg gacaggttga ctgcggcatt
    16561 gcccaagagg gaaatggttt caaacataca ttgggcgatg accggctcca tcacgtttaa
    16621 ttgcagttgc ccggcttcgg cggcgaaggt aatcgtcgtg tcgttgccga tgactttgaa
    16681 gcagacttgg ttgaccactt cgggaatcac gggattgact ttggcgggca ttatggaaga
    16741 accggcctgc aattcgggca ggttgatttc tttcaagccg gcacgcgggc cggaagagag
    16801 caggcgcaag tcgttgcaga ttttggagag tttgaccgcc gtgcgcttca atgcgccgtg
    16861 taccatcaca tatgcgccgc agtcggaggt cgcctcaatc aggttttcgg tcagtttgca
    16921 aggcaagccg ctgacttcgg agagtttttt gaccaccagt tcggcgtagc ctttgggcgt
    16981 gttcacgccc gtgccgattg ccgttgcgcc caaattgact tccaacagca gttggcgggt
    17041 gcggtcgagg ttgaggattt cttcttccaa caacacttgg aaagattgga attcttggcc
    17101 ggcagtcatc ggcacggcat cttgaagctg ggtgcggccc attttcaaaa cgtctttaaa
    17161 ctcttcggct ttggcggcaa aggcgttttt caggacggcc agtttgtcga gcaattcgcc
    17221 gatgctgtaa tacacggcaa ggcggaagcc ggtagggtag gcatcgttgg tggattggct
    17281 ggcgttgaca tgatccatcg gattgacgat atcgtagcgg cctttttcgt atcccaagac
    17341 ttccaatgcg aggttggcga tgacttcgtt ggtgttcata ttgaccgaag tacccgcacc
    17401 gccctgatac acatcggacg ggaattggtc gaggcagcgg ttgttcagca gaacttcgtc
    17461 gcaagccttt tcaatggcgg cggcgatttc gggcttgacc gcgcccaact taccgtttgc
    17521 ctgtgccgtc gcctttttca ccatcaccat actgcggaca aactgcggca cgtcagaaat
    17581 tttttgtgtg gagattttaa agttttcaat ggcgcgcagg gtgtggatgc cccaatacac
    17641 ttcggcggga atctcgcggt cgcccaataa atcgtgttcg atacggacag tcatgttttt
    17701 acctttgtaa gtcggataat taatattgaa aaaatgcgcc atcggaaaga tgccgccgca
    17761 ggacgaacac tataccggcc ggatgaaatt gtccatatcg tatgccgtct gaaaacggga
    17821 aacgttgttt tcgg
//
LOCUS       DAWHXS010000039        17142 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWHXS010000039 DAWHXS010000000
VERSION     DAWHXS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.039"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            279..1343
                     /locus_tag="WIE22_000992"
     CDS             279..1343
                     /locus_tag="WIE22_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ4127093.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1484..1807
                     /locus_tag="WIE22_000993"
     CDS             1484..1807
                     /locus_tag="WIE22_000993"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ4127094.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1879..2202
                     /locus_tag="WIE22_000994"
     CDS             1879..2202
                     /locus_tag="WIE22_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ4127095.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2249..2452
                     /gene="tatA"
                     /locus_tag="WIE22_000995"
     CDS             2249..2452
                     /gene="tatA"
                     /locus_tag="WIE22_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ4127096.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDGVIEHKKDEDKA"
     gene            2456..3142
                     /gene="tatB"
                     /locus_tag="WIE22_000996"
     CDS             2456..3142
                     /gene="tatB"
                     /locus_tag="WIE22_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ4127097.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3156..3926
                     /gene="tatC"
                     /locus_tag="WIE22_000997"
     CDS             3156..3926
                     /gene="tatC"
                     /locus_tag="WIE22_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ4127098.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3979..4584
                     /gene="maf"
                     /locus_tag="WIE22_000998"
     CDS             3979..4584
                     /gene="maf"
                     /locus_tag="WIE22_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ4127099.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAAEKNQTALALFLETNGAMPDFPLITADTCVVSDGIILGKPRSQAEAATFLNLL
                     SGTGHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4600..4953
                     /locus_tag="WIE22_000999"
     CDS             4600..4953
                     /locus_tag="WIE22_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ4127100.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4943..6757
                     /locus_tag="WIE22_001000"
     CDS             4943..6757
                     /locus_tag="WIE22_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ4127101.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVLLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPAPDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YTDEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6947..7624
                     /locus_tag="WIE22_001001"
     CDS             6947..7624
                     /locus_tag="WIE22_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ4127102.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7637..9043
                     /locus_tag="WIE22_001002"
     CDS             7637..9043
                     /locus_tag="WIE22_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ4127103.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAHPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SVDGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9161..11587
                     /locus_tag="WIE22_001003"
     CDS             9161..11587
                     /locus_tag="WIE22_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ4127104.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHYNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            11632..11877
                     /gene="rpsP"
                     /locus_tag="WIE22_001004"
     CDS             11632..11877
                     /gene="rpsP"
                     /locus_tag="WIE22_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ4127105.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11893..12402
                     /gene="rimM"
                     /locus_tag="WIE22_001005"
     CDS             11893..12402
                     /gene="rimM"
                     /locus_tag="WIE22_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ4127106.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12402..13151
                     /gene="trmD"
                     /locus_tag="WIE22_001006"
     CDS             12402..13151
                     /gene="trmD"
                     /locus_tag="WIE22_001006"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ4127107.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13167..13532
                     /gene="rplS"
                     /locus_tag="WIE22_001007"
     CDS             13167..13532
                     /gene="rplS"
                     /locus_tag="WIE22_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ4127108.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVISRRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13850..14575
                     /locus_tag="WIE22_001008"
     CDS             13850..14575
                     /locus_tag="WIE22_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ4127109.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14611..15486
                     /locus_tag="WIE22_001009"
     CDS             14611..15486
                     /locus_tag="WIE22_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ4127110.1"
                     /translation="MNPYDLLITPFAEFDFMRYALVSVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15563..16495
                     /locus_tag="WIE22_001010"
     CDS             15563..16495
                     /locus_tag="WIE22_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ4127111.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHDHEGHHHNHGEFDPHVWNDPVLMSAYAQNVAEA
                     LIKADPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRY
                     HIEFIAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNV
                     SGKLYSDALGNAPADTYIGMYRHNIKALTNAMKQ"
     gene            complement(16775..>17142)
                     /locus_tag="WIE22_001011"
     CDS             complement(16775..>17142)
                     /locus_tag="WIE22_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127112.1"
                     /translation="GFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSASE
                     QDNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGG
                     NVDANIQSVQQPLLVTPSA"
BASE COUNT         4480 a         5413 c         3756 g         3493 t
ORIGIN      
        1 ttagagaatc gttctctttg agctaaggcg aggcaacacc gtactggttt ttgttaatcc
       61 accataaatg ccatctgaag ccgattcggt tttcagacgg catcattatt tgacctgacg
      121 caaacagaaa gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat
      181 tcaactggaa acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat
      241 tcagacggta ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg
      301 ataaaggcga catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg
      361 gcatcaatgt cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc
      421 cgattttcat tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa
      481 cgatgggaca cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca
      541 aagtcggcca acacgtcgtg gtcgaaccct acatcatccg cgatgacgta ccgaccggag
      601 aaggcagcaa ctaccacctc tctaaagaca tgaacttcat cggcttgggc ggctgcggag
      661 gtggcttgtc tgaaaaaatc gctgtcaaac gccgttgggt gcatccgatt tccgacaaaa
      721 tcccgttgga tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac
      781 gcagcggcgc gaaagcaggc gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc
      841 tgttggctgc cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag
      901 cacgcaaaga caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag
      961 tcgatgttgt cgaagaggtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg
     1021 aatgtaccag cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca
     1081 atttggttat cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga
     1141 tgaaagagct ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca
     1201 aactggtcga agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg
     1261 acgaactggt ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa
     1321 ttattgtgag tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa
     1381 acgctcagac ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt
     1441 agcctatcat tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact
     1501 atccgccatc caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt
     1561 cgcacagctc ttgcacaagg gcgaggacaa aatcctaaaa aaagtgattg aagaggcggg
     1621 cgaagtgttg atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga
     1681 cttatggttt cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt
     1741 ggacgaactt gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga
     1801 atcttgaatt tatattaaaa tccgcacttt cccacattca atccgtctga ccgcttttca
     1861 gacggcaacg gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt
     1921 ccggcgcaaa ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgcc
     1981 gctccggttc atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg
     2041 cccgaacatc agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg
     2101 gcaggactgg cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag
     2161 gtcttccacc tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca
     2221 acccctaata cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat
     2281 cgtactgatt atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga
     2341 cctcggcggt gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga
     2401 agcccaaaaa gacggcgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt
     2461 tgatttcggt ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc
     2521 cgaacgcctg cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt
     2581 tgtcggcagc gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac taaggaaggc
     2641 caagcaggaa tttgaagctg ccgctgctca ggttcgagac agcctcaaag aaaccggtac
     2701 ggatatgcag aacagtctgc acgacatttc cgacggtctg aagccttggg aaaaactgcc
     2761 cgaacagcgc acgcctgctg atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc
     2821 ggcaaacacc ctatcagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc
     2881 cgccgaaacc cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga
     2941 ccaagaccgc gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac
     3001 cgtcgaagtc agctatatcg atactgctgt tgaaacgcct gttccgcata ccacttcgct
     3061 gcgtaaacag gcaataagcc gcaaacgcga ttttcgtccg aaacaccgcg ccaaacctaa
     3121 attgcgcgtc cgtaaatcat aaagagagca atccggtgtc cgaaacacaa aacgaacaac
     3181 ccgtccaacc gcttgtcgag catctcatcg agctgcgccg ccgcctgatg tggacggttg
     3241 tcggcatctt agtctgcttt ttcggcctaa tgccgtttgc ccaacaactc tatactttta
     3301 tcgccgaccc gctgatggca aacctgccca aagacaccag catgattgcc accgatgtca
     3361 tcgcaccgtt tttcgttccg gtcaaagtta ccctgatggc ggcattttta atttcgctgc
     3421 cgcatacgct ctaccaaatc tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac
     3481 gcctgattac gccgctcgtc ctctccagcg tcagcctgtt tttcatcggc atggcatttg
     3541 cctactttct ggttttcccc gtcattttca aattccttgc cagcgttacc cctgtcggtg
     3601 tcaatatggc gacagacatc gacaaatacc tgtcttttat cttaggcatg tttgttgcgt
     3661 tcggcacaac gtttgaagtc cccattgtcg ttatcctgtt aaccaaaatc agcgtggtaa
     3721 caaccggaca gctcaaacgc gcccgcccct atgtgattgt cggcgcgttt gtcattgccg
     3781 ccatcatcac gccgcccgat gtgatttcac aaaccctgct tgccattccg ctgattctct
     3841 tatacgaagc aggtatttgg ttcggacgct ttttcacgcc acgttcagaa caggatggcg
     3901 acatacagcc gcctgcaaca acctgacact atgccgtccg aacctccgcc tcataccgca
     3961 acagattaag gaataccttt gaataccctc tatttaggtt caaacagccc gcgccgaatg
     4021 gaaatcctga cacagttggg ctatcaggtc gtcaagctgc ctgccaacat cgacgaaacg
     4081 gtcagacaga acgaagaccc tgcccgttac gttcaaagga tggcggcaga aaaaaatcag
     4141 acggctttgg ccctgtttct cgaaaccaac ggcgcaatgc ctgactttcc cttgattaca
     4201 gcggatacct gcgtcgtttc agacggcatc atattgggca aaccccgctc ccaagctgaa
     4261 gcggcaacct ttctaaacct gctgtcggga accgggcata ccgtcctgac tgctgtctgc
     4321 attcattatc gcggcaaaac gtcaagccgc gtccaaacca accgcgtcgt tttcaagccc
     4381 ctgagttcgg aagaaatttc cgcctatgtc caaagcggcg aaccgatgga caaagccggc
     4441 gcctacgccg tacaaggcat aggcggcatc tttatccaat ctatcgaagg cagcttcagc
     4501 ggcattatgg ggctgcccgt ttatgaaacc gtttcgatgt tgcaggattt gggataccgt
     4561 accccacctt tcatccgagc ctaacccgta aagaccgccg tgaacagaca aaccgcttac
     4621 ctccttacct ctttcagcct gatcgcactg ataatcctgt ccctttcctg ggaactgtgg
     4681 attgcaccgt tgcgcccggg cggctcgtgg ctcgcgctca aagccctccc cctctgtctg
     4741 ccgctttcag gcatcttgaa aaagaaaatc tatacctacc aatacagctc catgctggtt
     4801 ctgatttact ttgccgaagc cgtcatgcgc ctgttcgacg cctatcccgc agaacagatt
     4861 tgcgccgcgc tttccgcagt attcagcatc atcttcttca tatcctgcct gtcgttcgtc
     4921 aaacaataca aggaaacaaa caatgcccgc tgaaacgacc gtatccggcg cgcaccccgc
     4981 cgccaaactg ccgatttaca tcctgccctg cttcctttgg ataggcatcg tcccctttac
     5041 cttcgcgctc aaactgaaac cgtcgcccga cttttaccac gatgccgccg ccgcagccgg
     5101 actgattgtc ctgttgttcc tcacggcagg aaaaaaactg tttgatgtca aaatccccgc
     5161 catcagcttc cttctgtttg caatggcggc gttttggtat cttcaggcac gcctgatgaa
     5221 cctgatttac cccggtatga acgacatcgt ctcttggatt ttcatcttac tcgccgtcag
     5281 cgcgtgggcc tgccggagct tggtcgcaca ctacggacaa gaacgcatcg tgaccctgtt
     5341 tgcctggtcc ctgcttatcg gctccctgct tcaatcctgt atcgtcgtca tccagtttgc
     5401 cggctgggaa gacacccctc tgtttcaaaa catcatcgtt tacagcgggc aaggcgtaat
     5461 cggacacatc gggcagcgca acaacctcgg acactacctc atgtggggca tactcgcctc
     5521 cgcctacctc aacggacaac gaaaaatccc cgccgccctc ggcgtaatct gcctgattat
     5581 gcagaccgcc gttttaggtt tggtcaactc gcgcaccatc ttgacctaca tagccgccat
     5641 cgccctcatc cttcccttct ggtatttccg ttcggacaaa tccaacaggc ggacgatgct
     5701 cggcatagcc gcagccgtac tcctaaccgc gctgttccaa ttttccatga acaccattct
     5761 ggaaaccttt accggcatcc gctacgaaac tgccgtcgaa cgcgtcgcca acggcggttt
     5821 cacagacttg ccgcgccaaa tcgaatggaa taaagccctt gccgccttcc agtccgcccc
     5881 gatattcggg cacggctgga acagttttgc ccaacaaacc ttcctcatca atgccgaaca
     5941 gcacaacata tacgacaacc tcctcagcaa cttgttcacc cattcccaca acatcgtcct
     6001 ccaactcctt gcagagatgg gaatcagcgg cacgcttcta gttgccgcaa ccctgctgac
     6061 gggcattgcc gggctgctga aacgccccct gacccccgca tcgcttttcc tgatctgcac
     6121 gcttgccgtc agtatgtgcc acagtatgct cgaatatcct ttgtggtatg tctatttcct
     6181 catccctttc ggactgatgc tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt
     6241 caaaaaagcc gccaatctcg gcatactgac cgcctccgcc gccatattcg caggattgtt
     6301 gcacttggac tggacataca cccggctggt taacgccttt tcccccgccc ctgacgacag
     6361 tgccaaaacc ctcaaccgga aaatcaacga gttgcgctat atttccgcaa acagtccgat
     6421 gctgtccttt tatgccgact tctccctcgt aaacttcgcc ctgccggaat accccgaaac
     6481 ccagacttgg gcggaagaag caaccctcaa atcactaaaa taccgccccc actccgccac
     6541 ctaccgcatc gccctctacc tgatgcggca aggcaaagtt gcagaagcaa aacaatggat
     6601 gcgggcgaca cagtcctatt acccctacct gatgccccga tacaccgacg aaatccgcaa
     6661 actgcccgta tgggcaccgc tgctacccga actgctcaaa gactgcaaag ccttcgccgc
     6721 cgcgcccggt catccggaag caaaaccctg caaatgaccc gcgccggcgg atacggatac
     6781 cgcccgaaat gtaaagctcc atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat
     6841 acgctatcaa aaaacattgc agccgtgtta agataaaccg tcaaacaatc ttttcacacc
     6901 ccgcccgaaa cagggtcggg gcataccctt acgaaaagga aacaccatga gccgcgtatt
     6961 actcgtagat gacgatgccc tgctgaccga actgctgacc gaatacctga gcgccgaagg
     7021 tctgaacgtc cgcagcgttc ccgacgggga agcaggcgta caggaaatcc tgagcgggca
     7081 gtacgatgta gtcgtattgg attccatgat gcccaaaatg aacggcttgg atgtcttgaa
     7141 aaacgtacgc gcccgaagca ccgtccccat catcatgctg accgccaaag gcgacgacat
     7201 cgaccgaatc atcggcttgg aaatgggcgc ggacgactat gtccccaaac cctgcacacc
     7261 acgcgaactc ttggcacgca tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca
     7321 gaacaacgca cccaacagca tctccgtcag cgatgtcgtc ctgtaccccg ccaaacgcca
     7381 ggcatccgtc aaagacatgc cgctcgaact gaccagcacc gaattcaacc tgctcgaagt
     7441 cctgatgcgc catgccggac aggtagtcag caaagaaacc ctgtccgtcg aagcactcga
     7501 ccgcaagctg gcaaaattcg accgcagtat cgacgtacac atctccagca tccgccacaa
     7561 gttgggcgat gcctctctga ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa
     7621 ctgaagcaac gggcaaatga aactgttcca acgcatcttc gccacatttt gcgcagttat
     7681 cgtctgtgca atctttgtgg cgagtttttc tttttggctg gtgcagaaca cccttgccga
     7741 aaaccagttc aaccaacgcc gcaccatcga aaccactttg atgggcagca tcatttccgc
     7801 attccgggca cgcggggacg cgggcgcgcg cgaaatcctg acggaatgga aagacagccc
     7861 cgtctcatcg ggcgtgtacg ttatacaggg cgacgagaaa aaagatatcc tgaaccggta
     7921 tatcgacagc tacaccatcg aacgcgcccg gcttttcgcc gccggacacc cgcattccaa
     7981 cctcgtccat atcgaatacg accgcttcgg cgaagaatac ctgttcttca ccaaagactg
     8041 ggacaaactc caagcccgcc gcctgcccag ccccctgttg atccccggtc tgccgctcgc
     8101 cccgatttgg cacgaactca tcatattgtc cttcatcatc gtcgtcggac tgctgatggc
     8161 gtacatcctc gccggcaata tcgccaaacc catcagaatc ttaggcaacg gcatggacag
     8221 ggtggcaaac ggagaacttg aaacccgtat ctcccaacag gtcgacgacc gcgacgacga
     8281 attgtcccat cttgccatcc aattcgacaa aatggtggaa aaacttgaaa aactcgttgc
     8341 caaagaacgt cacctgctcc atcacgtctc ccatgaaatg cgttctcccc ttgcgcgcat
     8401 gcaggcaatt gtcggactga ttcaggcgca tccccaaaaa caggagcaat atctcaaacg
     8461 gctggaaggc gaactgaccc gcatggatac gctggccggg gaactgttaa ccctgtcccg
     8521 tctcgaaact tccaatatgg ctttggaaaa agaaagcctg aaactcctgc ccttcctggg
     8581 caacctggta gaagacaatc aaagcattgc ccagaaaaac ggacaaacgg ttaccctgtc
     8641 cgtcgacgga aaaatccccg aaaacacaac catccttgcc aacgaaagct acctgtaccg
     8701 cgccttcgac aacgtcatcc gcaacgccgt caactacagt cccgaaggca gcaccatcct
     8761 gatcaacatc ggacaagacc acaaacactg gataatcgac gttaccgaca acggccccgg
     8821 cgtggacgaa atgcagctcc cgcacatctt caccgctttc taccgtgcag actccagtgc
     8881 gaacaaaccc ggaacaggac tggggcttgc attgacccaa catattattg aacagcactg
     8941 cggcaaaatc atcgccgaaa acatcaaacc gaacggtctg cggatgcgct ttatcctgcc
     9001 caagaaaaaa accggttcca aaacagaaaa aagtgcgaac tgaccataat accgtctgaa
     9061 ccggcttcag acggcattgc acaaacagtt actccaaaac aaacacacag cgggtgcatc
     9121 tccaacaatc acgcacatac cgtccgcaaa ggaatatatc atgtcggcac aaaccgatcc
     9181 gggctacttt ttcatgccca accacattat cctgataggc gcgagcgaac gtccgtacag
     9241 cctgggcgaa cgcgtactca gcaacctgct gagtacgccc tttcaaggaa aaatcacccc
     9301 cgtaaacccg cgccaccaca ccatagccgg gcttcccgcc tacaccagcc tcaacaaaat
     9361 ccccggcaat gcagacctga ttattgccgt taccctaccc gacagttacg acaccctctt
     9421 caaaacctgc cgcaaaaagc agctccgaca catcatcctc atacaggact gggacagcct
     9481 gtctgccgca gaactgcaca ccgccgaaac tgccatccgc aaacaccacg gcaacggact
     9541 caacatcacc gcctgcacca ccgcaggcat ccaactgccc tcactcggac tcaacatcag
     9601 tacccaagac ggatacgccg caggccatac cgccatactg accggcaatg ccgccgtcag
     9661 ccgccaaatc gacaacatcc tgaacaaact ccgtcaaggc acatcccgcc acatcagcct
     9721 gcatcccggc atcagcccca tcacttcctc agactggctc aaccgcttcg gccacagcct
     9781 gcacaccaaa accgccgtcc tacactacaa ccctgaagag gatcagcgca aactgttcag
     9841 cgcaatccgc caatttaccc gccatacgcc gctgattctc cacatcacct gccgcacgac
     9901 agaaaccgac cgtgccgtac tgcactgcct cgcccgacac tgcaacttcc tcatcagttt
     9961 caacgccgac gacctcgaag ccgcactgcg cgcccaactg tccgaccttc ctcccctgtc
    10021 ccgactcgac atcctgtccg acacgcctgc cgaatggctg aacgcgcacg cgccaaaaac
    10081 cctcgccctc cactttccca accttcccca tcacatccgc aacggacacc tgaccggcac
    10141 acccacaccc tcaatctgcc acgacatcgc ctcacgtcag cttgcccgcc ccgacaccca
    10201 agccgtccta accatcctca gtccctccgg acacgaggat tacaaaaaaa cagcacgcgc
    10261 ccttatccgc ctgtccgaac agaccgccaa acccctgctc gtcagcagcc ccttttcaga
    10321 cggcataaca catttcgaca cccccactca ggcaatccgc accctttcct accgcaacac
    10381 cgccgccgcc ctgaaacagg cacagctcga cattgcaccg ccgcagccat gccgtctgaa
    10441 aacaccgcaa ccccaaaaca tcaaaaaagc ccttgcagcg gcaaatccct ccctgctcgc
    10501 cgaagccctg cacctccccc cctaccggca cactacccat aacgccgtcc aattccaatt
    10561 cggcagccac cccctctatg gcgacatcct gaccgcatgc tgcaacggac aaaccactgc
    10621 cgtactcccg ccgtttacca cgctcgacag ccgccacctt gcccgctttg ccgaactcga
    10681 cggcacacaa accctcgacc agttcctgca cacactgacc gtcattcccg aataccgcca
    10741 acacattctc ggcatcaccc tcaacctcaa cggcggacaa tacagcagcg atttcatctt
    10801 aaagacaccc gaaacacacg acacgcccaa acgcaagaac acaggcaaag ccgcccaaac
    10861 cctcgaacat gccgccgcaa aaatgcagag tgccgccgca tacctgaaac acaaaaaccc
    10921 gacagccgcc gaatttctcc gccacacaag cgaagccgcc gcagaactgc tcggcagcaa
    10981 aaccgaaacc ggagcagccg tacccaacgt acttgccccc tatcccgcag cacacctcaa
    11041 aacactgtcc ctaaaaaaca acacgaccgt taccattacc ccccttttgc ccgaagacgc
    11101 agaagccaaa cagcagttcg tccgcagcct cagccccgaa gcacggtaca cacgcttcat
    11161 gacccacacc aacgaactgc ccgcagccac gttggcacgc ctgtgcaacc ccgattacca
    11221 ctgtgaagcc gcatggacgg caagggatgc cgacagcaac atcgtcgccg tcgtccgcca
    11281 cagccgcctg aaccgcaacg aatgcgaatt cggcatcaca ctggcggaac atatgcgcgg
    11341 cagcggactg gcacagaaaa tgatggaact catcatccaa accgccgcac agcaaggcta
    11401 ccggactatg agtgccgaca ttctcaaaac caatactccc atgatcaaac ttgctgaaaa
    11461 atcaggattt accctcaagg aatcggacac cgaaaaaaac ctgtaccgcg catatctgaa
    11521 ccttgcggca gacaaaacaa cagaaaaaac aaataaaaac ttgcacaccg accacaaaat
    11581 aacctaaaat cggcagtttc catatatccg attttctcaa aaggactcaa aatggtagtt
    11641 atccgtttgg cacgcggcgg ctcgaaacac cgccccttct acaacgtcat cgttactgac
    11701 tcacgcagcc gccgcgacgg ccgcttcatc gaacgcgtag gcttctacaa ccccgtagcc
    11761 aacgaaaaac aagagcgcgt ccgcctcaat gcagaccgcc tgaaccactg gattgcacaa
    11821 ggcgcgcaag tcagcgactc cgttgcaaaa ctgattaaag aacaaaaagc cgcctaatcc
    11881 gcgtttgccg ccatgacaga cactcaaaac cgggtagcca tgggctacat caaaggcgta
    11941 ttcggcataa aaggctggtt gaaaattgcc gccaacaccg aatattccga cagccttttg
    12001 gactaccccg agtggcattt ggtcaaggac ggcaaaacca tcagcgttac ccttgaagcc
    12061 ggaaaagtcg tcaacagcga actccaagtc aaattcgaag gcataaacga ccgcgacttg
    12121 gcattctcat tgcgcggtta caccatcgaa ataccccgtg aagcattcgc cccgacagaa
    12181 gaagacgaat actactggac agacttggtc ggcatgaccg tcgtcaacaa agaccatacc
    12241 gttttaggca aggtaagcaa cctgatggaa accggcgcaa acgacgtatt gatgattgac
    12301 ggagaacacg ggcagattct gattccgttc gtttcccaat atatcgaaac cgtcgatacc
    12361 ggcagcaaga ccattactgc cgactggggt ttggactact gatgcttatc caggcagtta
    12421 ccattttccc cgaaatgttc gacagcatta cccgctacgg cgtaacggga cgcgcgaaca
    12481 gacagggaat ctggcagttt gaagcagtca atccccgaaa gtttgccgac aacagattgg
    12541 gctacatcga cgaccgcccg ttcggcggcg gcccgggaat gattatgatg gctccgccgc
    12601 ttcatgcggc aatagaacac gccaaaacac aatcctccca agctgcaaaa gtcatctacc
    12661 tcagccccca agggaaaccg ctgacacacc aaaaagcggt agaactggca gaacttccgc
    12721 atctgattct gctgtgcgga cggtatgagg gcatagacga aaggcttctg caaagcagcg
    12781 tcgatgaaga aatcagcatc ggagacttcg ttgtttccgg cggagagctt cccgccatga
    12841 tgctgatgga tgcggtattg aggctcgtac ccggcgtatt gggcgatatg cagtctgccg
    12901 aacaggattc gttctcaagc ggcattttgg actgccccca ctacaccaaa cccttagaat
    12961 ttcaaggtat ggctgttccg gaagtattgc gttccggcaa tcatggcttg atagcggaat
    13021 ggcggttgga acaatcgctg cgccgcacct tggagcgcag acccgatctt ttggaaaagc
    13081 gcgttttaat cccaaaggaa tcccgcctct tagaaaccat ccggcaagag caacgggaaa
    13141 tccaatcata atttaggaaa aaaacaatga acctgattca acagctcgag caagaagaaa
    13201 ttgcccgcct gaacaaagaa atccccgaat tcgcaccggg cgacaccgta gtcgtatccg
    13261 tacgcgtcgt ggaaggtacc cgcagccgtc tgcaagccta cgaaggcgtt gtcatttcac
    13321 gccgcaaccg tggtctgaac agcaacttca tcgtccgtaa aatttccagc ggcgaaggtg
    13381 tggaacgtac tttccaactg tactccccta ccgttgagaa aatcgaagtc aaacgccgtg
    13441 gcgatgtccg ccgtgccaaa ctttactatc tgcgcggtct gaccggcaaa gctgcacgca
    13501 ttaaagaaaa attgcctgca cgcaaaggtt aataagcgat ttcccccaat gccgtctgaa
    13561 ccttcagacg gcatttctta ttgctgcgat gccggcaagt caacgatatg cctgcccctc
    13621 atccaccttg attaacaatc actcccgcct attcaagcat aatttacaga ccaaacgtta
    13681 tacagtatca tatcagccat ataacgataa acgctttacc gccttccctt tcaaaaccgc
    13741 aaatcatcat gacaccttcc cttttactat caggattgac tttccgcctc atccttgccc
    13801 tgattgccgt atccctttta tggggcgttt acttttgggc agtatccgca tgagcatcat
    13861 tgtcgaaaac ctgaccgtca gctaccgccg ccgacctgcc gtgcaccatg tggacattac
    13921 ttttgaagaa catagtatgt gggcggtttt cggtcccaac ggcgcaggga aatccacctt
    13981 tctcaaatcc ttaatgggat tgcagcctat cgatacgggc agcatccggc tggacggatt
    14041 gacccgtcag aacattgcct accttcccca gcagtccgac atcgaccgct cgcagcctat
    14101 gaccgttttc gacttggcgg caatggggct atggtatgaa atcggttttt tcagaggaat
    14161 aaatgccgtt caaaaacaac gcgttcacga atctttggaa cgcgtcggga tgcggcagtt
    14221 tgccggccgc caaatcgccc acctctcaaa cggacaattc cagcgtgtcc tttttgcccg
    14281 aatgctggtt caaaacgcca aattcctact tctagacgaa cctttcaacg ctgttgatgc
    14341 acggacgacc tacgaacttc tcgacgtatt gcagaaatgc cattgcggcg gacacgccat
    14401 catcgccgta ctgcacgatt acgaacaagt ccgtgcctac tttcccaata ccctgctgct
    14461 cgcccgcgaa aaaattgcgg caggcgcaac cgagaccatt ctgacagaac cctacctcgc
    14521 ccaagccaac gccaaaatgc agcaacagga aagccccgag tggtgcgcct cataaatgcc
    14581 gtctgaaacc atgtccccgt accaaacact atgaacccct acgacctgct tatcacaccc
    14641 tttgcagaat tcgactttat gcgctacgcc ctcgtatccg tcttctgcct gtccctcagt
    14701 gccgcacccg tcggcgtatt cctcgtcatg cgccgtatga gcctgatagg cgacgcattg
    14761 agccacgctg tcctgcccgg tgccgccgtc ggctacatgt ttgccggctt gagcctgccc
    14821 gccatgggtt tgggcggcgt agccgcaggc atgctgatgg cactgcttgc cggacttgtt
    14881 agccgcttca ccaccctgaa agaagatgcc aactttgccg ccttttatct cagcagcctc
    14941 gccataggcg tagtcctcgt cagcaaaaac ggcagcagcg tcgatctgct gcatctgctt
    15001 ttcggctctg tacttgccgt cgatattcct gccctgcagc tcatcgccgc cgtatccacc
    15061 ctcacactgc ttacccttgc cgtcatctac cgcccgctcg tactcgaaag catcgacccc
    15121 ctgtttctca aatctgtcgg cggcaaaggc gggctttggc acgtcctctt tctcgtcctg
    15181 gtcgtcatga acctcgtatc cggctttcaa gccctcggca cactcatgtc cgtcggactc
    15241 atgatgctgc cagccattac cgcccgcctg tgggcgaagc acatgggcgc actcatcctc
    15301 ctatccgttc tgacagccct gctgtgcggc ttgagcgggc tgctcatttc ctaccacatc
    15361 gaaatccctt ccggccccgc catcatcctc tgttgcagcg tcctttatct cttttccgtc
    15421 atactcggca aagaaggcgg cattctgacc aaatggctca aaaaccaccg ccaccacacc
    15481 acctgacccc gcaagccgct tgaacaaaca ggcggcttgt tttaccataa cagatgttac
    15541 gttatatcac aggagaacag ttatgaaaca cctcaaactc acccttattg ccgcattgct
    15601 aaccaccgcc gcaactgctg cccccctgcc cgtcgtaacc agcttcagca ttttaggcga
    15661 cgttgccaag caaatcggcg gagagcgcgt atccatacaa agtttggtcg gagccaacca
    15721 agatactcat gcctatcaca tgaccagtgg cgacattaaa aaaatccgca gtgcaaaact
    15781 cgtcctgctc aacggcttag gacttgaagc cgccgatgta caacgtgccg tcaaacagag
    15841 caaagtatcc tatgccgaag cgaccaaagg catccaaccc ctcaaagccg aagaagaagg
    15901 cggacaccat cacgaccacg atcatgacca cgatcatgac cacgaccacg aaggacacca
    15961 ccacaaccat ggcgaattcg acccgcacgt ctggaacgac cccgtcctca tgtctgccta
    16021 tgcccaaaac gtcgccgaag ccctgataaa ggccgacccc gaaggcaaag tttattatca
    16081 acaacgcttg ggcaactacc aaatgcagct taaaaaactg cacagcgacg cacaagccgc
    16141 atttaacgcc gtccctgccg ccaaacgcaa agtcctgacc gggcacgatg ccttttccta
    16201 tatgggcaaa cgttaccata tcgaattcat cgccccgcaa ggcgtgagca gcgaagccga
    16261 gccttcagcc aaacaagtcg ccgccatcat ccgccaaatc aaacgcgaag gcatcaaagc
    16321 cgtatttacc gaaaacatca aagacacccg catggttgac cgtatcgcca aagaaaccgg
    16381 tgtcaacgtc agcggcaaac tgtattccga cgcactcggc aacgcgcccg cagacaccta
    16441 catcggaatg taccgccaca acatcaaagc cttgaccaac gcgatgaagc aataaaaccg
    16501 tttgattggt ttgaatttga aaaggcaaaa ggccgtctga aaattatagt ggattaacaa
    16561 aaatcaggac aaggcgacga agccgcagac agtacagata gtacggtaag gggaggcaac
    16621 gctgtactgg tttttgttaa tccactatat tgctagaagt gcaccgatta aggttaatta
    16681 aaatacgacc cttatcccaa tattaagaaa aacagcaatc aaaataaact tgattgctgt
    16741 ttttcagttt aagccatgaa tgtgattagg ctccttatgc agatggcgtt accaataacg
    16801 gctgctgtac ggattgtata ttggcatcca catttcctcc tgtcgcagca gtattagaac
    16861 cgaacacaga catagactgt accaaattat tgccagcatt aatatacttg gcaaaatctg
    16921 gattactgat agctgcatca tcaaagacaa atgtatctac acgatggttt tcaccataga
    16981 agaatccgga aatacgtacg ttgtcttgtt cagaagcgct aagcaccaaa tcacttccgg
    17041 aacggataaa atgaacatct gctgctttaa atcctttaaa gtgcatagtg tcagagtttt
    17101 tatccacatg gtaattatag accgtatcct gaccgaagcc tt
//
LOCUS       DAWHXS010000040        17047 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWHXS010000040 DAWHXS010000000
VERSION     DAWHXS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17047
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.040"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..801
                     /locus_tag="WIE22_001012"
     CDS             <1..801
                     /locus_tag="WIE22_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4127113.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQV
                     LLDFLSKDDIDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIKEKLFFEKGIYSEDVEWSMRLFNAATTFSYLDGCYYYYRQGRE
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            842..1372
                     /locus_tag="WIE22_001013"
     CDS             842..1372
                     /locus_tag="WIE22_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ4127114.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1392..1522)
                     /locus_tag="WIE22_001014"
                     /pseudo
     CDS             complement(1392..1522)
                     /locus_tag="WIE22_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234705.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
     gene            complement(1541..1729)
                     /locus_tag="WIE22_001015"
     CDS             complement(1541..1729)
                     /locus_tag="WIE22_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
                     /protein_id="HEZ4127115.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTAIGHPVRMNLGLTQ
                     ERWPDIVFHETREHSVKL"
     gene            complement(1748..3343)
                     /locus_tag="WIE22_001016"
     CDS             complement(1748..3343)
                     /locus_tag="WIE22_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ4127116.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWTPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3450..3845)
                     /gene="hisI"
                     /locus_tag="WIE22_001017"
     CDS             complement(3450..3845)
                     /gene="hisI"
                     /locus_tag="WIE22_001017"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ4127117.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3876..4643)
                     /gene="hisF"
                     /locus_tag="WIE22_001018"
     CDS             complement(3876..4643)
                     /gene="hisF"
                     /locus_tag="WIE22_001018"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ4127118.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4656..5393)
                     /gene="hisA"
                     /locus_tag="WIE22_001019"
     CDS             complement(4656..5393)
                     /gene="hisA"
                     /locus_tag="WIE22_001019"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ4127119.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5426..6064)
                     /gene="hisH"
                     /locus_tag="WIE22_001020"
     CDS             complement(5426..6064)
                     /gene="hisH"
                     /locus_tag="WIE22_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ4127120.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGYRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6195..7697)
                     /gene="pta"
                     /locus_tag="WIE22_001021"
     CDS             complement(6195..7697)
                     /gene="pta"
                     /locus_tag="WIE22_001021"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ4127121.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7882..8940)
                     /gene="fbpC"
                     /locus_tag="WIE22_001022"
     CDS             complement(7882..8940)
                     /gene="fbpC"
                     /locus_tag="WIE22_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ4127122.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8961..10478)
                     /gene="fbpB"
                     /locus_tag="WIE22_001023"
     CDS             complement(8961..10478)
                     /gene="fbpB"
                     /locus_tag="WIE22_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ4127123.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10546..11541)
                     /gene="fbpA"
                     /locus_tag="WIE22_001024"
     CDS             complement(10546..11541)
                     /gene="fbpA"
                     /locus_tag="WIE22_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ4127124.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11792..12262)
                     /locus_tag="WIE22_001025"
     CDS             complement(11792..12262)
                     /locus_tag="WIE22_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ4127125.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGWEQELVLIW
                     RAEADIGNGGILQFVCNWSFPAAEKTCSVLKKIGAIHSAMLIHRAADALDKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12319..13695)
                     /gene="argH"
                     /locus_tag="WIE22_001026"
     CDS             complement(12319..13695)
                     /gene="argH"
                     /locus_tag="WIE22_001026"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ4127126.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMSA"
     gene            complement(13714..14583)
                     /gene="galU"
                     /locus_tag="WIE22_001027"
     CDS             complement(13714..14583)
                     /gene="galU"
                     /locus_tag="WIE22_001027"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ4127127.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14611..15210)
                     /gene="rdgB"
                     /locus_tag="WIE22_001028"
     CDS             complement(14611..15210)
                     /gene="rdgB"
                     /locus_tag="WIE22_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ4127128.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELAGKTDKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHREQALADLIRKLAL"
     gene            complement(15203..15436)
                     /locus_tag="WIE22_001029"
     CDS             complement(15203..15436)
                     /locus_tag="WIE22_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127129.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15544..16077)
                     /locus_tag="WIE22_001030"
     CDS             complement(15544..16077)
                     /locus_tag="WIE22_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ4127130.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(16179..16637)
                     /gene="nudB"
                     /locus_tag="WIE22_001031"
     CDS             complement(16179..16637)
                     /gene="nudB"
                     /locus_tag="WIE22_001031"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ4127131.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16848..16924
                     /locus_tag="WIE22_001032"
     tRNA            16848..16924
                     /locus_tag="WIE22_001032"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16882..16884,aa:Pro,seq:ggg)
BASE COUNT         3477 a         4101 c         5217 g         4252 t
ORIGIN      
        1 cggacaccaa atatcaaata tatatatcag gataaccaag gattgtcaga ggcgagaaat
       61 accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcgga tgattttatt
      121 aattgtcagg tcttgctgga ttttcttagt aaagatgata ttgatatgcc ggatgtggtt
      181 tttttaaatg cagttaaata tgataaggga agtgtttcat attttggcga agattatcag
      241 cctgaaaaaa tactcaatca atccaaagtc gaagttttga aaggattatg ccgatttaga
      301 aaatttccgg gttcggcgtg gaataagatt ataaaaagag aattgattat taaagaaaaa
      361 ctgttttttg aaaagggaat ttattctgaa gatgtcgaat ggtcaatgag attatttaat
      421 gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca gggaagagaa
      481 gattctatta cgggaactgt ttcggaaaaa agtataaagt cattattata tattttggag
      541 aaaaatgcgg aaatggaatt taatagggat atatcaagtt atctttattc ttttctttcc
      601 tacgaatatc tcgttttgct ttttatcatg acgagtaaaa atatagcgtg tgatgctgat
      661 ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa attgatatat
      721 aagctgatat tcccgataat cacattattc ggggttgata ttacaggcag gattttaaaa
      781 gcaatcaggg ggaatattta ataaatcctt taacactata catcttaccg aaggaggaaa
      841 aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa gctgtatgat
      901 agacgaagcg tgcgtcgtca tcggcgaagt gtcgcttgcc gaagacgttt ccgtgtggcc
      961 gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca
     1021 ggacggcagc gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag ggtcgccgct
     1081 ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgtatcgg
     1141 caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga ttgaggacga
     1201 agtgatgatt ggcgcgggca gcctcgttcc gccgcgtaaa cgtttggagg gtggctatct
     1261 ttatgtcggt tcgccggtca gacaagtgcg cgtgctgacc gatgaggaaa aagccttttt
     1321 gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat
     1381 cggcgttctt gcgtcagccc caaattcatg cggacgggac gcatccgata acggtatccg
     1441 atgcgccttg attttgaccg actgcgtttg ggttgcaggc aaaaatgccg tctgaaagcc
     1501 ttttttcggg ttcagacggc attttattgc cgattgcttt ttaaagtttg accgaatgct
     1561 cgcgcgtttc gtggaacacg atgtccggcc aacgttcttg cgtcagccct aaattcatgc
     1621 ggacgggatg cccgatagcg gtatccgatg cgccttgatt ttgaccgtct gcgtttgagt
     1681 tgcaggcaaa aatgccgtct gaagcccttt tttcgggttt agacggcatt ttattgccga
     1741 ttgcttttta aagtttgacc gaatgctcgc gcgtttcgtg gaacacgatg tccggccagc
     1801 gttcttgcgt gagtcccaga ttcacgcggt tgggggcgag gtaggcgagg ttgccgcccg
     1861 cgtcgatggc gaggttgccc gcgttggctt tttcaaattc cgccagtttt ttcttgtcgt
     1921 cgcacgatac ccagcgcgcc gaccagatgg atgcgctgtc gaacacggct tccacgccgt
     1981 attcgttggc aaggcgcgag gtaacgactt caaactgcaa cacgccgacc gcgcccaaaa
     2041 tcaaatccgc gccgctcatt ggtttgaaca cctgtaccgc gccttcttcg ccgagttgtt
     2101 gcaagccttt ttgcagttgc ttgattttca gcgggttttt gatgcggacg ctgcggaaca
     2161 gttcgggcgc gaagaatggg atgccggtga acgccagttg ttcgccttcg gagaagctgt
     2221 cgccgatttg gatgttgccg tggttcggga tgccgatgat gtcgccggcg taggcttctt
     2281 ccaccagctc gcggtcgtgc gacatgaagg ttaccacgct ggaggcggcg atttcgcggt
     2341 tgatgcgaag gtgtttcatc ttcataccgc gctcgaatct gccggagcag acgcgcagga
     2401 aggcgatgcg gtcgcggtgt ttcggatcca tattggcttg gattttaaag ataaatccgg
     2461 agaatttcgg ctcgtccggc tcgaccatac gtacggtcgc gtcgcgcggt ttcggcgcgg
     2521 gcgtccagtc aatcaatgaa ttgaggattt cctgaatacc gaagttgtta atcgcagagc
     2581 cgaagaatac gggcgtgagt tcgccggcga ggaattcgtc gagattaaac tcgttggaag
     2641 ccgcctgcac caattcgatt tcgtcgcgca actgctggat ttccaatgga aagcgttgtt
     2701 ccaattcggg attgtcgatg cctttgatga tgtcgaactc gtgcggcaga cgttcgccac
     2761 cggcttcaaa gagatagatt tcgtcgttca ggatgtggta cacgcctttg aagtttttgc
     2821 ccatgccgat cggccaggtt acgggcgcgc agcggatttg caggatgttt tccacttcgt
     2881 ccagcaactc caaagaatcg cgcacttcgc ggtcgtattt gttcatgaac gtgacaatcg
     2941 gcgtattgcg caggcggcag acgttcaaga gtttgatggt ttgcgcttcc acaccttttg
     3001 ccgcgtcgat gaccatcaac gcgctgtcca cggcggtcag aacgcggtag gtgtcttcgg
     3061 agaagtcttg gtgtcccggc gtgtccaaga ggttgacggt gtggtctttg tagtcgaact
     3121 gcatcacgct tgatgccacg gaaatgccgc gctgcttctc gatgtccatc cagtcggagg
     3181 tggcgaattt gccggttttt ttgcctttta ccgtacccgc gctctgaatc gcgcccgaaa
     3241 acagcaacag cttttcggtc agcgtggttt tacccgcgtc ggggtgggag atgatggcaa
     3301 acgtgcggcg gcggcgcact tggtcgagga tttcttggga catggttttc tttgcaaaaa
     3361 ggttcaggcc gcttttcaga cggcccggac agtgtttgag acggcgaaat tgtacaaaaa
     3421 aatgcctgat aattcaatgt tggaggcggt cagtgcgtgc tgccgtaaat ctctttttcg
     3481 tctttcagga cggcatcggc ggtttcccac gcgccgccgc gccagacttt gtaaaagcag
     3541 ctttctcgcc cggtgtggca ggcgatgccg ccgttttggg cgatgagcat cacaatggcg
     3601 tcgccgtcgc agtcgaggcg cagttcgcgg actttttgcg tgtgtcccga ctcttcgccc
     3661 ttcatccatt gtttttggcg cgaacggctg taatagtggg caaagccggt ttcgacggtt
     3721 ttttgcaggg cttcggcgtt catccacgct accattaaaa cacgtttggt ttcggcatct
     3781 tgggcgatgg cgcaaaccaa acctttttcg tcaaatttga cggcttcaag caggttttta
     3841 tccatatttc ctttcagacg gcatggtcgg ggcggtcaga ggcgcacttc gatgccggct
     3901 tcgcgcatag cgcgtttggc ttcgcggatg gcgatttccc cgaaatggaa aacgccggcg
     3961 gcaagtacgg catcggcttt gccttcggtt atgccttcaa tcaggtgccg gacattgccg
     4021 acccctccgg aggcgatgac ggggatgtcg acggcttcgg caacggcgcg ggtcagcggc
     4081 aggttgaaac cctgtttcgt accgtccctg tccatcccgg tgagcaggat ttcgcccgcg
     4141 ccgcgttttt gcatttcgat cgcccattcc accgcatcca aaccggtcgg attgcgcccg
     4201 ccgtgggtaa agatttccca gcgtgtgttt tcggagttga cggctttggc atcgacggcg
     4261 gcgacgatgg cttgcgaacc gaaaaatccg gcggcttcgt caatcaggtc gggacgggta
     4321 acggcggcgg tgttgatgct gactttgtcc gcgccggcat tgagcaggcg gcggatgtcg
     4381 gcaacggtgc gtacgccgcc gccgacggtc agggggatga agacttgtcc ggcaacctct
     4441 tcgatgatgt gcaggatggt gtcgcggttg tcggatgagg cggtgatgtc gaggaaggtc
     4501 aattcgtccg cgccttcgcc gttgtagcgt ttggcggctt cgacggggtc gcccgcgtcg
     4561 cgcaaaccga tgaagttcac gcctttgacg acgcgcccgt cttttacgtc gagacagggg
     4621 atgatgcgtt ttgccagtgc cataatcgga tgcctttagt cgagggaatc tgccagttgc
     4681 tgcgcttggg caaaatcgat gctgccctcg taaatcgcgc ggccggtaat cgcgcctgct
     4741 acgccatgtt tttcggcggc acacagggcg cggatgtcgt ccaagccggt cagtccgccg
     4801 gaggcgatga cgggaatgcg gacggcttgg gcgagtttga ccgtcgcgtc gatgttcacg
     4861 ccgctcatca taccgtcgcg cccgatgtcg gtgtagatga tgctgttgac gccgtcgtct
     4921 tcaaagcgtt ttgccaaatc aattacatga tgcccggtta cggttgccca gccgtcgatg
     4981 gcggccatac cgtctttggc atccagcccg acaataatcc tgccggggaa ggctttgcac
     5041 gcctcgcgca cgaagtcggg gtttttgacc gccgccgtgc cgataatcac gtcgtttaag
     5101 cccaaatcca aatattgtcc gatggttttc aaatcgcgta tgccgccgcc gagctgtacg
     5161 gggatgtctt tggcgacagc ggcaaggatg tctttgatgg cgggcaggtt ttgcggaacg
     5221 ccggcaaacg cgccgttcaa atctaccaga tgcaggcggc gcgcgccttg tttgaaccag
     5281 tgcagcgcgg tttcggcggg cgaatcggaa aagacggtcg cctcttccat cagcccttgt
     5341 ttcaggcgga cgcagcgtcc ttctttcaaa tcgatggcgg gtatcagcag cataattttt
     5401 ctccttgtgc ggggccgtgt ccggcttacc agtttaaaaa gtttttcaac atcgtcagcc
     5461 cggcatcgtg gcttttttcg gtgtgaaatt gcgtggcgaa tacgttgtct ttgccgacga
     5521 tgcaggcaaa cggggacggg tagtcgcttt cgcccaatat ggtttcggga ttttcggggg
     5581 cgaaatagta gctgtggacg aagtaaaaac gcgtgtcttg gggaatatct ttaaacagcg
     5641 ggtggttttg ggtttggcgc acggtgttcc agcccatatg cgggactttc agacggtatc
     5701 cctgcgggtc gcggaggtcg cgctcaaagc gtctgacttt gccgccgaac cagcccaagc
     5761 cgtcggtgtt tccttcttca ctgtggtcga ataaaagttg cgcgccgacg cagattccga
     5821 aaaagggttt gttttttaag gcatctttga ctgcctcgtc caggccgccg cgcgtcagtg
     5881 ccgccataca gtcgggcatc gcgccctgcc cgggaaaaat gactttgtcg gcgcgggaca
     5941 cgcgttcggg gtcgccgctt aaaaagattt cggtattttt tccggcaagc tgccccgccg
     6001 tccggacgga tttcaatacg gaatgcaggt tgcccatacc gtaatcgata atggcgattt
     6061 gcatggcttc ctcctctttt tttgcaatat ggctgcgatt ttaacaaaca aatgtgccgt
     6121 gctgataaaa atgccgtctg aaaacgggag tctgtcttca gacggcatag ggttcaaacc
     6181 cggaaagccg tttgtcagcc ttccatttgt tttgcctgaa cggcagtcag ggcgatggtg
     6241 aacacgatgt cttccaccag cgcgccgcgc gacaggtcgt tgaccggttt gcgcaggcct
     6301 tgcagcagcg ggccgacgct taagacgttg gcactgcgtt ggacggcttt ataggtgcag
     6361 ttgccggtgt tcaggtcggg gaagaccaaa acggttgcct gtcctgccac cgggctgccc
     6421 ggggctttgg atttgcccac gcccggcacg gttgccgcat catattgcag cgggccgtcg
     6481 atggcgaggt cggggcgttt ttcccgggca agtttggttg cttcgatgac ggtatcgaca
     6541 tcggggccgc tgccggagtt gacggtggag taggaaatca tcgccacttt cgggtcgatg
     6601 ccgaaggctt ttgcggaatc ggcagactgg atggcgatgt cggcaagctg ttgcgcggtc
     6661 gggttcggat taaccgcgca gtcgccgaag acgaggactt ggttgggcag cagcataaag
     6721 aatacgctgg acacgaggct tgcgcccggt gcggttttaa tcagttgcaa agcggggcgg
     6781 atggtgttgg cggtggtgtg aaccgcaccg gataccaaac cgtccacatc attttgcgcc
     6841 atcatcatcg taccgagtac cacggtgtct tgcagttgct tgcgcgcgtc ttcgggtgtc
     6901 aggcctttgg atttgcgcag ttcgcacatc ggctcgacgt attgttcgac caatgaggcg
     6961 ggatcgatga tttccaaaga gtcgggcagg ctgatgccgc gttctttggc aacggcttcg
     7021 acttcttcgc gtttggcaag caggacgcag cgggcaatgc ctttttcgtg gcagatggcg
     7081 gcggcttgga cggtgcgggg ttctgcgccc tcaggcagga cgatgcgttt gtcggcttgg
     7141 cgggcgaagt cgatcaggtt gtagcggaat tgcgccggcg acaggcgttt tgcttcgcgg
     7201 cctgccaata cggatacgtc tttcagcgcg tcgctcgaac cgaagaaggt caggccggtt
     7261 ttttcggctg ccgcttcggc aacggaggct gccgcgccgt ccacgacaaa accttccaat
     7321 acgcccggcg cggcggcgaa gaactgtttg gcaaggttca gccgatttgc cagttcgtcg
     7381 gcatcggcgt tgtcggaacg gacggcgaag acggctgccg cgtcaaggga caatgccagt
     7441 tcgacgtttt tgcctgcgag gtagattttg tcggcatcgg gcgcgatgcc ttcgatgacg
     7501 aggttggcgg catcgagtgc ggcaactttg ccgaccagtg cgtcgaacca gtcgtcgctt
     7561 ttgccttgcg cgagcagggt ttcggcggtt gcgtcaacgg cttggaaaat ttgtgcgtcc
     7621 agtgcttttg caaaggcttg tgcggcggcg gaggcgtcca gtccggcaga tacgggtacg
     7681 atgagtactt ttgccatgat atatcctttc gtatgctgcg gtgtgcggca tatgtggttg
     7741 gaaggggcgg catataggca gaaacggctg cctgcgtgcc gtgcgtgccg tgtttggctt
     7801 gaggcgcgca ggttgaatat agcaaacaaa ttctgtttcc aacaagataa atatccgcag
     7861 gcttgtggat gctgccgcct ttcagagggt atttccgggg aagaacaggg cgggaccgtc
     7921 caaatggagg acggcggaaa tgccgtctga cagggtgggg gcggaaggga ggttgagcgt
     7981 gaggacggtt tgtccgaccc ggagactgat ttcggtatgc cgcgctttgg gcgtggtttt
     8041 gagaaccacg gcgtgaatgg aggcggtggg tgcggaatgg gggtgaaggc tgaactgttc
     8101 cggacggatg agcagtgtgc cgcgtgtgcc tgcgggtgcg ccgctttgaa cgggcaggcg
     8161 gcccaatccg caatcggcgg tgccgtcggc gttgagcgcg gcggggaaca cgatgccttc
     8221 gccgataaac agggcggcat caaggtcggc aggttgtcgg tacaattcgt gagggcttgc
     8281 agtttggagg atgcgcccct gtttcatcac ggcaatccgg tcggcgtatt gcagggcttc
     8341 ttcgcggtcg tggctgacga aaacggcaga tttgccgttg gcgcgcaggg cggcaatcat
     8401 gtcttcgcga atctggcggc gcaactgttc gtccagcgcg ctgaagggtt cgtccaacaa
     8461 aatcagttcg ggatcgggtg caagggcgcg ggcgagggcg acgcgctgtt gctgtccgcc
     8521 cgaaagttcg tgcggatagc gtccggcaag ttcggaaatg ccggtcaatt ccaacatagc
     8581 ttcgatgcgc tgccgttctt gcgccgtctt gcctttcccg ttgcccagcc cgtaggcggt
     8641 gttgcggtaa acggtcaggt gggggaacag cacaccttcc tgtacgacat aacccaaacg
     8701 gcgttcgcgg acggggaggt tggtattttt cgagaagatg gttctgccgg aaagcgaaat
     8761 ttcgccaaaa tcgggttgtt caaaaccggc aaggcagcgt aaaagggtgg ttttgccgca
     8821 gccggacgcg ccgacgataa agaggatttc gcccgggtcg aggctgagcg aaatgtcgtt
     8881 taaaactggg gtgttttgaa aacttttgga caggtgtccg atgtgcaggg cggcggtcat
     8941 ggcggtactt cctcaagctg ttatttgaag gcgtatttct tcagcaggaa tacggggatg
     9001 ccggaaaata ataccagcat cagcgcgtaa ggggtggcgg cggcgtattg tgcgtccgat
     9061 gtgtattccc aaacggcggt ggagagtgtg tggacatcgt cggtggtcag cagcagggtg
     9121 gcggtcagct ctttcatcag tttgaggaag acgagtgcga atgcggcggt aatgccgggc
     9181 aggatggacg gcagtaccaa cgtcctgaaa ataaagaagt gtccgcgccc caatgttgcg
     9241 ccgacctgtt ccatcccttt ggggagttgt tccaaggaag tcctcagggt ggtttgcgcc
     9301 atcggcaggt aaagcatgaa ataggcaagg atgacgacga taaaggtttg gtaaacggca
     9361 ggggtgtagt tgatgctgaa ataaaccaag gatagggcga taaccaaacc ggggacggcg
     9421 tgcagtaaaa acggcagcct gtctatccaa acggttaaaa aattgcgata gcgaaccgat
     9481 gcccaaacaa ggggcaaggc acataatata gtcaaaatcg cacctaaagc cgatacgctt
     9541 aaggaacgga taaaggcatc aaatacggat acgagcgcga atgtgccgga agtgccgacc
     9601 atcatccaat gtatcaatac gccaaagggg ataataatgc ccaaagtcaa caagctgctt
     9661 aaaaaaacaa tcgcgccgat ctgaccgggc agtttgaggg ttttgacggg ataaggacgg
     9721 gcaacgcctt tgccgctgtg gtaaatcttg gctttgccgc gaaatatgct ttctccaaat
     9781 acgacgatgc cgcacaccgc cattaaaaca gcggaaagca gggcggcggt attgttgttg
     9841 taggacattt cgtattcttg gaaaatggcg gtggtaaaag tggggtagtt caaaatagat
     9901 accgcgccaa attcgaccag catatgcagg gcaatcagta acacgctgct gccgatggcg
     9961 ggtttgagct gggggaggat ggcggaaaaa aaggtttgca ggcggctttt gcccaaggac
    10021 aggctgactt cttcgtaaga caggctgatg cgtttgagtg ccgcctcgac gggcaggtag
    10081 gcgagcggga acgaggacag gctcataatc atcactgtcc cccaaaagcc ttcgacacgg
    10141 aaggtcaggc tgatccaggt gaaacagctg acaaatgcgg ggatgcacaa aggcagggtg
    10201 attgccgtct gaaaaaaggt tttgccgaag aagcggtaac gttggaacaa aagggcgcag
    10261 gcaatgccca aaacaatgga aatcagggta acgcccgcca tcatcgtcaa ggtgttggag
    10321 agcaaatccc acatacgcgg gcggaacaac agttcgacgg cgcggttgat gccgacctgc
    10381 cacgaacgca tagcgacata taaaaaaggc agggtaagcg gtagggcaat cagtaggatg
    10441 aggccggtaa gccaaatggg tattttttta ggagacatag tgttttttat cggcaaaacg
    10501 ggcggacagt ataaatgtcc acccgtttga caatccgaaa acggcttatt tcataccggc
    10561 ttgctcaagc agccgggtgg cgtgttcttt ttcggaaaca gtggtggcgg acacttgggg
    10621 tgcttccaac ttggcgatgg gttccaaatt gaaagtggat accacgtgcg gattcaaagg
    10681 atattcggca cggacggcgg tcagggcgcg ctgtccttcc ttgctggcga ggaaggcgac
    10741 gaattttttc gcctcatcct tgttttggga ggattttaac acggctgcgc cggaataggt
    10801 aacgagtgcg ccgggatctc tgtggcggac gaaattcagg cgggtgtgga cattttgtac
    10861 gcctttttca cgcgcaaaag cgtgccagta gtagttgttg atgagggcgg catcgatttc
    10921 gccgttttca accgcttgaa gggcgacgga gtttttagcg taaggcttgc cgtattcttt
    10981 cagacctttg agccatttca atgcggccgc ttcgcctttc agtttgacga tggcgacaac
    11041 ctgttccaag aacgcgccgg aagtgggggc gtaaccgatg cggtttttcc atttcggcgt
    11101 ggcgtaattc aggacggatt tttccaaatc tttttcagac agtttgcggg tgtcgtaaac
    11161 gacgacgcgc gaacgtccgc tcagtgccac ccagtctttt ttggcggcaa ccggcacgcc
    11221 cttgccgcgt gtttcgttga tggtggaggc gggcaggggc tctaggaggt tggctgcgga
    11281 aagggtggcg agtgccggga tttgttcgga atagaatacg tcggcggggc ttcggctgcc
    11341 ttcttctttg atttgaccgg caagctggtc gcctttggcg ctgttgagtt tgactttgat
    11401 gccggtagcc cgggtaaagg catctgcaac ggcttgtgcc gcttctttgt gttggccgtt
    11461 gtacacggta atgtctgcca gcgcgggggt tgcggcggtc agggctgcgg caagcagtgc
    11521 gtatcggata gatgttttca tatcgatttt ctcctaatga atgagagtgt acaccttgtt
    11581 aagacataac ggcgtgtagt gtattccttc ttctttataa atgcaaataa ttatttttta
    11641 aatttgttat tatccgatcc ggttatggtt tgttctgact tgtatttttt ccgtgcatcg
    11701 cgcccgtaag gcggaagcgg cgggcaatgc gtggcggaat gtgggtaaag gcggcatttt
    11761 gatttgtcgg aatgcttgag aacccctctc tttaaaacag cgtctgaaca gggttgccgg
    11821 aatagtattg ccatcccggc agatacagtt tgtcggggtc ttgccaatat tgttcatcca
    11881 gactgttcgg cagtgaggcg gttttgttgt cgagatgttt tgttatatcc cacatttctt
    11941 tcaggttttt accttccgat tggaggtggc ggatttcttt gtccaatgcg tctgccgccc
    12001 gatggatcag cattgcgctg tgtatcgcgc cgattttttt cagaacggaa caagtctttt
    12061 cggcggcggg gaaactccag ttgcagacaa attgcagtat gccgccgttg ccgatatcgg
    12121 cttctgcccg ccaaattaaa accagctcct gttcccaccc gtccatgctt tcgagtttgc
    12181 cgtcatgctg ttcaaaaagt ttgtccaccg cctgccacat catctgttcg aagcggtcgg
    12241 cttgggtatc cgtatcggtc atagtgttct tgtcttttaa aaatgccgtc tgaacatttc
    12301 ttcagacggc atcgggggtt aagctgacat ttcccgccag cgtttgactt ggaaacggac
    12361 ttgttcaggc gcggtaccgc ccaagtggtt gcgcgcgttt aagctgcctt cgggtgtcag
    12421 cacgccgtaa acgtcgtcgg caatcaaatc gctgaaacct tgtaagactt cgagcggcag
    12481 tttgctcaaa tcgacacccg cttggtcggc gtggcgcacg gcttgggcga cgacttcgtg
    12541 ggcatcgcgg aaaggcatgc ctttttttac caagtaatct gccaaatcgg tggcggtagc
    12601 gaagccctgc atcacggcgg cgcgcatatt gtcgggtttg acggttacgc cgcgcatcat
    12661 atcggcgtaa atccgcaacg tgtcgataag cgtgtcggcg gtgtcgaaca agggttcttt
    12721 gtcttcctga ttgtctttgt tgtacgccaa gggctgcgat ttcatcaggg taatcaagcc
    12781 gataaggtgt ccgatgactc ggccggattt gccgcgcacg agttcgggca cgtcgggatt
    12841 tttcttctgc ggcatgatgg acgaacctgt gcagaaacgg tcggcgatgt cgataaagcc
    12901 gaaacgcgga ctcatccaca aaatcaattc ttcagacagg cggctcaggt gaaccataac
    12961 cagcgaggcg gcagccgtga actcgacggc aaaatcgcgg tcggatacgg catcgagcga
    13021 gttttggcag atttgctcga aacccagtaa ttcggcggta atttcgcgcc gaatcgggta
    13081 ggtcgtaccg gcaagggcgg ctgcgccgag cggcatacgg ttgacgcggc ggcggcagtc
    13141 cgccatccgt tcgttatcgc ggccgagcat ttcgacgtag gcgagcatat ggtgtccgaa
    13201 gctgacgggc tgggcgactt gcaggtgggt aaagcctggc atgacggttt cggcgttttg
    13261 ttccgccaaa tccagcaatg ccgtctgaag gctttgaatc aggcttcgga tgacggtaat
    13321 ctggtcgcgc agccaaaggc ggatgtcggt ggcgacttgg tcgttgcggc tgcggccggt
    13381 gtgcaggcgt ttgcccgcgt cgccgatttt gtcggtcagg cggcgttcga tgttcatgtg
    13441 gacatcttcc aaatcggacg accattcgat tttgccgctg cggatttctt cgaggatttc
    13501 cgccatgccc cgacggatgt ccgccaaatc gccttcgtcc aacacgccgg tttctttcag
    13561 catttgcgcg tgggcaagcg agccttggat gtcccattcg gcaaggcgtc ggtcgaaacc
    13621 gatggaggcg gtgtattgtt tgacgagttc ggaaacgggt tcgttgaaac gtccggacca
    13681 ggttttgtcg tgcataagga ttccttgatg tggttattcg gtacggtatt tttccaaaag
    13741 gcggcggaag ggttcgccgg tttcggggtg tttcaggccg taggcgacgg tggcttccag
    13801 atagcccagt ttgctgccgc agtcgtagcg cgtgccttca aaggggtgcg ctaggacaaa
    13861 ttcgtgatcg agcagcttgg cgatgccgtc tgtaagctgg atttcgttgc ccgcgccgcg
    13921 cggcagtccg gtcagtaagt cgaaaatgcg cggggtgagg atgtagcgtc caacaacggc
    13981 aaggttggag ggcgcgtctt cgggcttggg tttttcgaca atgccggtaa tgcgttggaa
    14041 ctgtttgagc tgttcggttt cgacgatgcc gtatgagccg gtttgcgatg cttcaacggt
    14101 ttctacgccc aaaatgctgt tgccgctgcg cccgtacact tcgaccattt gtttgagcgc
    14161 gcctttgggg gcatcaatca ggtcgtcggc aaggataacg gcaaagggtt cgtctccgat
    14221 ggcggcgcgg gcgcacaaga cggcgtgtcc caagcccagt gcttccgcct gacggatgta
    14281 gaggcaggta atgttcggcg gcaggatgtt gcggacgtgt tccaacaatt tgtctttatg
    14341 gcgcatttcc aactcggttt cgagttcgta tgccttgtcg aaatggtctt cgatgctgcg
    14401 tttgttgcgt ccggtaacaa acaccatttc cgtgcagccg gcttccacgg cttcttctac
    14461 ggcgtattgg atcagcggct tgtcgacgat gggcagcatt tctttcgggc ttgccttggt
    14521 ggcgggcagg aagcgtgttc ccatccctgc gacggggaag acggcttttt ttatcggttt
    14581 cattcttttt cctttgtatt gttttgatgt ttaaagggcg agtttgcgga ttaaatcggc
    14641 gagtgcctgc tcgcggtggc tttcgcggtt tttgacctcc gaatccaatt cggcggcggt
    14701 tttgccgtgt ccgggcagat aaaaatacgg gtcgtaaccg aaaccgtttt caccggcggg
    14761 aacgccgttc cattgtccgt gccatacgcc ctcggcgata attggacgcg ggtcgtcttt
    14821 atggcggaca aaaaccaata cgcagacgta gcagcagctt ttgtcggtct tgccggcaag
    14881 ttcggcggca agtttcaggt tgttggcggt atcggatttg ggattgtcgc ccgcgtaacg
    14941 tgcggaatgg atgcccggcg cgccgtttag ggcggcggca cagatgccgc tgtcgtcggc
    15001 aagcgcgggc agcccgctgt attcggcggc atggcgtgct tttgccagcg cgttttcgac
    15061 aaaggtggga tagggttcgg ggcattcggg tatgccgaat gcggattgcg gcaatacggt
    15121 gatgctgtaa ggtttgaata agttgccgaa ttcttcgagc ttgccggcat tgccgcttgc
    15181 caaaacgatt ttttccggtt tttcagacat agcggttttc ctttgcggcg gattgggcgg
    15241 cgcgtaggga tttgtgccgc aggtagaggg cgaggctgcc gatttgtccg aacagtgcgc
    15301 cgaatgcgaa aagaaaggag gcgacggcga aggctttgct gccgacggtc agcagataag
    15361 cacccaagag gacgagcagc ataaatgcca gtgtgaagag ggcaacagac aacagataga
    15421 tttttcggcg gttcatggcg ttcggtcgga aacggtatgt tcggattata gccgattggg
    15481 acggtattcc ctagagcttg gaaaaatgcc gtctgaaacg gcgttcagac ggcatggcgg
    15541 gcgttatgcc tgtttgttcc aacgttcgat ggattctttg atgacttttt tcgcttcttc
    15601 cgcatcgccc cacgattcga ctttggtcgt acctgctttt ttcaggtctt tgtaatggtt
    15661 gaagtggaac tcgatttgtt tgatcagctg ttgcggcaaa tcggacaaag ttttgtaggc
    15721 gttgccgttg ttgcggtcgt cggcaggaac gcagacgatt ttgtcgtcca cttcgccgtc
    15781 gtcaacgaat ttcatcacgc cgataacgcg cgcttccaag aatacgccgg ttgccaaagg
    15841 ttgttcggta acgagcagca cgtccaattc gtcgccgtct tcgtccaaag tttggggaat
    15901 gaagccgtag ttggtcggtt tggcgaagat ggcgggttcg acgcggtcga gttggaatgc
    15961 ggccagtttg cggttccact cgattttgtg gttgctgccg gcggggattt cgttgacgac
    16021 gttgatgatg ccgccgtcca catcgccggg ggtcaggatt tggttgaagt ctgccatttg
    16081 gtttcctttg tttgggaaag tttgaagttt gaaagtatag cacaaacgtc cggctgaaaa
    16141 tgcgcccgat gccgtctgaa agggtgtacg ggcgcgtgtt accgtttgcc caaaaacctg
    16201 cccagttcca gaatcgcgcg cctgttggac ggggaaaaca ctttttccgc cgcttcttcc
    16261 aagccgaacc agccgtagga gacgtgttct tcgggttgca gggcgatggg cgtatcacgc
    16321 gggatttcgg cagagaagag gtgttcgcgg ttttcaaaca cgccttttgg atagcggtgc
    16381 cgccagtggt ggtagatttc gtaaaccgtg ctgtcgtgcc agtcttgaag ctgcccgtcc
    16441 gccagcagga tgccggtttc ttcccaaact tcgcgccttg ccgtttgggc gacggtttcg
    16501 cccggttcga ggctgccggt taccgactgc caaaatcctt ccggatgcgt gcgttcgatg
    16561 agcaggatgc cgccgtcccc gctataaagg acgaccagtg cggagacggg gtatttgagc
    16621 ggttttgcca tcggcatctt tcggcgggct gcggtaatga aggggcggat tatagcaaac
    16681 gccgcacgtt atggcgttta tccttttccg tatccttttt tctgcacgga tgggacgcgc
    16741 cggtgtttgc cggtaaattt tccgttgtgt caaaaagata agggcggttg tgattttaat
    16801 gcttgccaaa gcgtcgggcg gaaactataa tccgaaactt atcgagtcgg agtgtggcgc
    16861 agtctggtag cgcacttgca tggggtgcaa ggggtcgaag gttcgaatcc tttcactccg
    16921 accaaaaatt ccgaaagccg ctttcaaaag cggctttttt gccgtccgta tgattatgat
    16981 gtagagtacg cggcgacaga cattcaaatg ccgtccgaaa accgttcaga tggcatttct
    17041 ttatctt
//
LOCUS       DAWHXS010000041        16236 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWHXS010000041 DAWHXS010000000
VERSION     DAWHXS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.041"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..261
                     /locus_tag="WIE22_001033"
     CDS             <1..261
                     /locus_tag="WIE22_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ4127132.1"
                     /translation="SKYFGQSMALIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNI
                     WKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
     gene            203..643
                     /locus_tag="WIE22_001034"
     CDS             203..643
                     /locus_tag="WIE22_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127133.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(1143..1218)
                     /locus_tag="WIE22_001035"
     tRNA            complement(1143..1218)
                     /locus_tag="WIE22_001035"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1183..1185),aa:Asn,seq:gtt)
     gene            complement(1311..1670)
                     /locus_tag="WIE22_001036"
     CDS             complement(1311..1670)
                     /locus_tag="WIE22_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ4127134.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(1693..2337)
                     /locus_tag="WIE22_001037"
     CDS             complement(1693..2337)
                     /locus_tag="WIE22_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ4127135.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(2339..2875)
                     /locus_tag="WIE22_001038"
     CDS             complement(2339..2875)
                     /locus_tag="WIE22_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ4127136.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(2887..3984)
                     /locus_tag="WIE22_001039"
     CDS             complement(2887..3984)
                     /locus_tag="WIE22_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ4127137.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTSSQSQSRGLLQRLMKKLLGS"
     gene            complement(3987..5423)
                     /locus_tag="WIE22_001040"
     CDS             complement(3987..5423)
                     /locus_tag="WIE22_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ4127138.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(5643..6425)
                     /gene="bioC"
                     /locus_tag="WIE22_001041"
     CDS             complement(5643..6425)
                     /gene="bioC"
                     /locus_tag="WIE22_001041"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ4127139.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(6413..7060)
                     /locus_tag="WIE22_001042"
     CDS             complement(6413..7060)
                     /locus_tag="WIE22_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ4127140.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(7070..8212)
                     /gene="bioF"
                     /locus_tag="WIE22_001043"
     CDS             complement(7070..8212)
                     /gene="bioF"
                     /locus_tag="WIE22_001043"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ4127141.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            8435..9511
                     /locus_tag="WIE22_001044"
     CDS             8435..9511
                     /locus_tag="WIE22_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ4127142.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(9718..11184)
                     /locus_tag="WIE22_001045"
     CDS             complement(9718..11184)
                     /locus_tag="WIE22_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ4127143.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11382..12305)
                     /gene="speB"
                     /locus_tag="WIE22_001046"
     CDS             complement(11382..12305)
                     /gene="speB"
                     /locus_tag="WIE22_001046"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ4127144.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGIIDPAHSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(12404..14296)
                     /gene="speA"
                     /locus_tag="WIE22_001047"
     CDS             complement(12404..14296)
                     /gene="speA"
                     /locus_tag="WIE22_001047"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ4127145.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(14463..14747)
                     /locus_tag="WIE22_001048"
     CDS             complement(14463..14747)
                     /locus_tag="WIE22_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ4127146.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(14898..>16236)
                     /gene="aspS"
                     /locus_tag="WIE22_001049"
     CDS             complement(14898..>16236)
                     /gene="aspS"
                     /locus_tag="WIE22_001049"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ4127147.1"
                     /translation="FIDIETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQL
                     LMVAGFDRYYQITKCFRDEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKD
                     ALGVDLGDFPRMPYSEAMFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGG
                     RVVALRVPNGAKFSRKEIDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVK
                     FLSENALKEIIARTGAQNGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEW
                     KPLWVVDFPMFEYDEEADRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWE
                     IGGGSIRIHRADVQEKVFAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVT
                     LMTGAESIRDVIAFPKTQRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
BASE COUNT         4305 a         4067 c         3686 g         4178 t
ORIGIN      
        1 agcaagtatt ttgggcaatc gatggcatta attgctcaga aagaatttga agaatttgtt
       61 aataacgtaa aagagtacga tattttagag agtttctctt atttcttaaa taagagagtc
      121 gcacataata tttggaagat ttatttttct gatgaatctg ttatttttat aagaaaatca
      181 gaagagaacg gaaaaactgt tcatgaattc gtttatcaag agtacaccga tagttctgat
      241 tttaattcta tgttcgagta attcttattc tgaacctgta agattagaaa gatcacaaat
      301 taaatttcaa tcaagtaata atttaaaatc aagtggtttt aaattagatt caagttctaa
      361 gagttttgcc aaattcatag aagcggcaaa ttttgagcac atccctacgg gcgcaaaagc
      421 ccgaatcaac gccaaaataa ccgccagcgt ttcccgcgcc ggcgtattgg cgggggtcgg
      481 caaacttgcc cgcttaggcg cgaaattcag cacaagggcg gttccctatg tcggaacagc
      541 ccttttagcc catgacgtat acgaaacttt caaagaagac atacaggcac gaggctacca
      601 atacgacccc gaaaccgaca aatttgcaaa ggtctcaggc taagtgcgcc tgttgccgcc
      661 taaaaggtaa cccggatgcc tgattatcgg gtatccgggg aggattaagg gggtatttgg
      721 gtagaattag ggagtgattg gtagcggaaa tagacgaaaa cctgtgtttg ggtttcggct
      781 gtcgggaggg aaaggaattt tgcaaaggtc tcaattagta tagtggatta acaaaaatca
      841 ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca ctcggtgctt
      901 cagcacctta gagaatcgtt ctctttgagc taaggcgagc caatgccgta ctggtttttg
      961 ttaatccact ataaaatcga gtttatccta gctgtccagg acagccccta ttttttcata
     1021 accatcacga aaggaatttt gcaaagattt tattccatct caaaacaatc atctcaaaaa
     1081 tgcgtttctg accgccggta aaacaaaacc ctctaagaaa atacttagag ggttttgaaa
     1141 tttggctccc cgacctgggc tcgaaccagg gacctgcgga ttaacagtcc gtcgctctac
     1201 cgactgagct atcggggaat aagcgcgaat aataaaacag aaataccgaa cgtgtcaata
     1261 taaattgaca atcgattgca ctttatctgc aagcccgcaa tctttatatg ttatatggct
     1321 tccgcaatat gccttgccgc acgtttggca tccaatagga ttaaaccaag tctgccggtt
     1381 tcttttgcca ccagcaccaa cacggcatct ttacccgcct gacttaaaag gatatagcct
     1441 gattttcctt taatcatcac ttgttccaat tccccgcagg cgagttcctg caccgagcga
     1501 ctccccaaag caagcaaagt ggcagaaatc gcccctaccc tgtccgaatt caaatgtgaa
     1561 ggaagcattg tcgccatcgg caatccgtcg gttgagataa cggcagacgc gataatatcc
     1621 gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata attgctgcat aacccctcct
     1681 ctctcccaag ttttacacgc ggttgctgta acggcggtat aaaatcctta ccaaagtaac
     1741 aaatgcctct ttgcccaaat cgggaatgcc gccgataacc aaaataaatt tggttgaacc
     1801 gatatacaat gggaaaaatg tcaattcgct ctgaccggaa ggatcgcaaa cgccccaagc
     1861 gttattgttg atatacaggt tgttcttaat cagcagccgg tatttctttt ccatctgtgc
     1921 gacttctgcc gccaacaacc ccaactcttc cgccgcctca tgatggaaat tggcgttggc
     1981 aagatacaga ccgttccgat cgaccaataa cgccttaccg ctgccggaca attgttccat
     2041 cagcaacggc aattgctcgt ccgacaaatt gatgccgtct gaatgaccgt tttcatcgcc
     2101 atagaggaat tcgagttttt gcaaacggta caacaggttc aaagcggtat cgatgtcggc
     2161 ggtgtccgcc caggtaagca gcttctcact gctgaccatt tcgtccgcat ccgctttcaa
     2221 caggctgtgc aacaaagttt taccggcact gggggcatcg ctggatacgg cataaaatgc
     2281 accggcagga gtcaggcggg gatataaatt tgcttgtagt gaaagtgttg attccatatt
     2341 aaacctccag tcccggatca atagaaaata acattgcgct aaccaattgt tttacgtcat
     2401 cttccttacg ggcatcaatt tcaaaaaccg gaacattaag attatgtttt gcaagatatt
     2461 tgtgatacac gtcgataccg ggctgagagc gtatatccat cttggtaata ccgacaacga
     2521 cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa gaattccaaa tctttcaacg
     2581 gattggttcg ggcattatct aaaagcaaga ccaaacccat actgccttgg cttaagattt
     2641 cccacataaa gttgaaccgt tcctgaccgg gcgtaccata taaatggact ttggtatcct
     2701 catccaagct gatggccccg tagtccatcg ccactgtcgt attccttttc ctatccaaag
     2761 tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc gtccgaaata gccgcaatgg
     2821 cagtggtttt ccctacgccg acaggtcctg tgaaaataat tttattttct ctcatctctc
     2881 cgcctcttag ctgcccaaca gttttttcat cagcctttgc agaaggccgc gcgactggga
     2941 ttgggatgat gttgtgattt tttccgcttt tttcatcatt tcactatcag aggcagaatc
     3001 ggctccgata tttattttat ccgccatatc ggagtatgcc tgttgtgaaa ccgtttttaa
     3061 atctaccgac aaaaacccgg ttgtataggt tgccgcaaga taattcagaa tatcattgag
     3121 gtttaaaggc atcactttat acaacacgtt aaggttgacg gatgccttgg tcagaaatgc
     3181 cgacaagcgt atcgaccccg gcacatttgc caaccgggtc aggtttggcc aagatttcaa
     3241 cgtaaacgga gtatcgggag aaatcggata aatcaacctg ccctgcgctg tccaaatgga
     3301 aaactgccac atacaggaca taatgcctac tttagccttt tcgcgccatt gcgggttatc
     3361 gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg cacaattctt cgagtttttg
     3421 cacactttct gtcagcaaaa cccgttgtat cgaggggaaa acaataagga ccggcttatt
     3481 tccatgcaag atagcgatgt cctgcctgtt cttttccgca aaccgcaacg cccccaataa
     3541 tcctttattc gggttaaact ggcgtatcgt aaccgtacgc tgcacattcc cgttattttt
     3601 cgccgaccgg tctgcgggtg caataaacga tttcccataa acattctcgc cctgcaacaa
     3661 tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt ttgggcaggt agggaacttc
     3721 agaatcggga actttctccg aacagacggc gacgggtata tccttataac gctcggcaag
     3781 ctctttccaa agttcaaaac cgccctcggc atcggtatcc gccaaaacca aatcgggcac
     3841 ggcactgccg tctgaagggg atactgtttc ataacgggtg gtattgtgca ttttgaatgc
     3901 cattttgaaa acggattcct gctgcgccgt catccccgcc aacattacgc gtactgtttt
     3961 aattttcggc agttgaactt ccatttttat tttccgtacc gttttatttt tttaatattt
     4021 tgattcatac gctgcagcag ccggctcatc agcataacga cctcttcagg aagcctgtcc
     4081 gcacgttccc tcaataccct taaaaactgc cccaacctat cccaatcttc agtacgttca
     4141 taaatatcga tcaacgtaat ataaagctgg gactcgtcgg gatatttcaa taccgcctgc
     4201 tccaacacat ccattgccgc ttcaatctga ccatacatca gcaacgactc tacttcctta
     4261 accgcatcgt ctgccggaga cgaaccggtg ttgatcaacg aagaatcttg aagcaccaaa
     4321 tcccgatgtt gcgatttgaa tttctgtata tttttcggca gatacccgtg ccccataccg
     4381 atgtctttga tttgccggtc gttcggccct ttttccaaat catcgaaaac ctcatggtaa
     4441 cccaggctgt acccccaccc cagcatccgc tctttaacct gcctgccgta gttgcccaac
     4501 gtttggtaaa gtttccacaa atgtccggca aaacggtcta tgtccgcgta ttggtaatcg
     4561 agtttcaacg catcgataat caggtttgca ggtttttcgg aagtttggat ggcacggtcg
     4621 tattgtttcg atgccgtttc atagctgact ttgtctttaa ggattttcgc accttgatcg
     4681 gcacggacca aaccggcaat cgcaccgatt tcctcttgac tgattccgga cacgtctttt
     4741 ttgccccgca caatcgggat gcgcttgatt tcttcggctt cataagcctt accgccggca
     4801 tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc cttccgcacg tttttcaatc
     4861 tcctgagtac cccatcccaa accttcttcc gccaagacgc ggatacgcaa atggtttgaa
     4921 tcgcgctgta atgcctgttc gatatatttt gccaaaagtt cggaaggaat cagtttgccg
     4981 tatttttgca gattgtctgc caaaacatcg acatccccca cttcgagatt gatatcgagc
     5041 agctcgcgga taaggttttc aggtttcgct tcaccatccg gaatgccgtc cagataggca
     5101 gccaaagatt cggcagcctt gccctgataa ccgaattgct tataaacctg atactccgta
     5161 agcggatcga cttcttgcgc ggatacggcg gcggatgcgg tctcggcact ttcgttccaa
     5221 gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa tctgatcgac ccagtccgaa
     5281 tcattgcttc cgggtgtctg cccctgcgta ttgccgacac cttgggattt gcgcttcggt
     5341 gttttcttac cctgcttcgc acgcataacc aaaagcatca gcaataccgc cagtgccaaa
     5401 ccgataatta atgagttttc caaaggatac tcccgatacc gtaacgagcg gatagctgac
     5461 cgcctgcaac ggccattgtc cgcttttatt attaacgatt tactgaaata gacttgtaag
     5521 ttttaaatca taccataatt taacgtttaa caatatgcct tctgcacaag cctcgccata
     5581 ttacctttta cccacatcag tatcaatacc cgatataaaa ataactttgc ccataagctg
     5641 ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa ataacggatg ataagtcagg
     5701 tgcactttgc ccgacggcat actgaacgcc gacaagtaat cgccaataaa tccattgaga
     5761 ttttttcttg tccaattttt ttggttcgtg gctgttacgc ctgtatattt aaggtgtttg
     5821 agtacatcta acggcgtatc aaagtctagt attaccttaa aatcctcaca ccataaaagc
     5881 tcaaaatctt tggctaacca agtctgccat tgggataaag tcgggtaatt taagcctata
     5941 tttgtaattt ggcggacttc ttttaaattg tctttgccaa aggttgcaac cgccaataat
     6001 ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag cgtcgggttg atgaaaccat
     6061 tgcacggcag atgcgcttgc gattaagtca aattgtcgtt gaaaaggaaa gttttccgca
     6121 tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag ccagttgggg ctgcacatcg
     6181 cacaaatcat taaataacca ataattcgct gaaatctgtt tttgcagcaa ggcactcaac
     6241 attcctgagc cgcagcccaa ttccaacacg ttttccaatg gcatatccgg caaataatct
     6301 tgcaaatgcg ccattagatt aatcgtcatt ttttgttgga ttaaggcgtg ccggtcataa
     6361 tcgtttaatg ctttttggaa agcctgccga atgcgcgatt tatttatggc agtcagtgat
     6421 tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta atgtccgccg tcaatttccc
     6481 gaacggtgca acgcggtgtc caatatcggt gctgattggc aggcataaaa attttatcgc
     6541 ccgatccgac caaggcattt gtccagcgaa tcaaatctgc gcgcctatct tgcccgatca
     6601 tcgcaaaaag tgcgataagt tcttgatgaa tttcgtcaaa cggacgggcg ggaaattgtt
     6661 ggtaatcttc aaaagatgct ttatcgccac acattctgcg ttcaaatttt aaacgggtgt
     6721 tttccgtgag gttctccaat gtgcctttaa aaacggcgca agggataccg aaattatcat
     6781 cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt taatcttatt ccttgcaatg
     6841 ccctctctgc cgcccaaacg cccattgacc acgccaccaa acggatgtgc cgataggcgg
     6901 aaaaatcaaa atccaaattt aaatcttgat aatcatagca aatcaataaa tcgtgatttt
     6961 ccggcaaaat caaatgattt acagcatcgg gcggcgttcc ccaacctgca aaatacagga
     7021 ttaaatgttc gccttgatga ttgtaaaatt ttgttttcat atcatatcct tacaggcacg
     7081 ccgcaaactg ccgcacttca tccattgtca tatctgccgt caaagacaat cgtattctgg
     7141 atgtaccttt tggtactgtc ggtggtctga tgggcaggca ataataaccc tgcctttgca
     7201 ggtattccgc tttggcaagg gtggcttcat tcccgcctaa aatatagggg acgatacagg
     7261 tttggctcgg cattatttgc gtccgatgcg ccacttcccg ccgtaaaaat gcgcttaact
     7321 gctcaagatg gcttctttct tttgagaatt gcggcaatcg ttcaaaaata aaataagtcc
     7381 aagccacatt aaacggcggc aatgcggttg aaaaaatcaa tgggcgcatt tgattaatca
     7441 aacattcttt caatacttgg ttgcagacgg catacgcccc caccgaggct aaggctttac
     7501 cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt atcccgttcg gcaatcccca
     7561 atccgttttg cccataaaca ccgattgcgt gggcttcatc cacataaaga taagtattgg
     7621 gaaactgttt ttttaattgg acaagctgtt tcaaatccgc cacatcgccg tccatactga
     7681 aaacagattc ggtaacgata aaagtgcggt caaattttcc gacgtttttt tcaagcagat
     7741 ttttcaaatg ttcataatca ttatgacgat aacgggaaaa cgcacaccgg ctcaaacgga
     7801 tgccgtcaat catactggcg tgaacaaatt tatctgccaa aatcaaactt ttcgtcgtcg
     7861 tcaaagcagg caaaataccg agattggcgt gatagccgct gttgaacaat aacgcgcttt
     7921 cccgttggaa acgttgtgcg acaagctctt ccaaatcggt ataaatagga aagttgcccg
     7981 ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt accgccgtat tgctgcaaaa
     8041 aagaccggcg caagttttca tctgatgcca aacccaaata atcattagac gacatattca
     8101 gcattttgca gttttcccgc gtaatatacc gcccttgatg aatcaaatcc ggaatcgaac
     8161 gatattggtt ttgcgcgccg agttgttcaa gctgttgttt aaaaaccttc attatgatgt
     8221 aaatattcct gagttaaagc ctgaaccaac ccagcatcaa acaatcaagt aatgcccttt
     8281 taataaaaac cgttttgccg ccgacctttg ccacacccct gccttcataa tccaaggcgg
     8341 aaatttccgc gacatttgtt tccgttgcca ttacccaatc cgtccgcact gtaccgaagc
     8401 gcatattttc gcacaaattg gtacggtttc aggtatgatt tgccgaaaac ctatcggaca
     8461 cacaccatat tttgatatga ataaaacaat ctgccgcact gccgcactat tgataagcgg
     8521 tttttcatac gcaaacaccg tcattcccga tgtctccccc gtcgcacaag gtcaacatgt
     8581 cttcattaac atccctcagc aacgcctgtt cctctacacc gacggcaaac tgaccaaggt
     8641 ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat ctaggcgaac ataaaatcgg
     8701 tgcgaaagcc tataaccctg tttggtacat ccccaaatcc atacagaaag aacgcggcga
     8761 cggcgtaaaa accatagccg ccggcccgga caaccctttg gggccggttt ttgtccgctt
     8821 aggcgacccc aaactcggtt tgggcataca cgggaccaat gcgccggcca gcgtccccgg
     8881 ggttcggagt cacggctgcg tccgcatgaa atcgcccgac gcgctcgagt ttgccaaaac
     8941 catcgccagc ggctcgcccg cctccgtcat ctatcaaatg gcggggctca atgaagatgc
     9001 ggatcgcaac ctgtggcttg ccgccttccg cgacccttac ggtaagaaca accttgacat
     9061 cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca cagggtaaaa ccatcgcgcc
     9121 cgagaaagtc gatgctgtac tcaaagaccg caccggatcg gccgtctgcc tgacctgcgg
     9181 caaaaacggc aagatgaaga tgccgctcaa atcgctggcg tggatacagg gttcttcctc
     9241 atacagccaa gctgaagtca ttgaacaaac tgaggaaacc aactctgccg aaatatccga
     9301 gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag gcacaaccgc atttaaatac
     9361 ccaatccgac ggcacgccga ctgcctatac cgaaccggct gccgattcat cgccgcaagt
     9421 agaaacacct gatcaggctg cttccgagcc ggttgatgta ttattttcaa tagatgtgat
     9481 acggcaggga aatttgcgtt taggtaattg aataaccttc tgattattta atgcttatgt
     9541 ttatcagaag ttgataggcg gtttggtttt tgtttggctt ttcctacacc gccgcctgcc
     9601 gctttctgca acattcaagc gcacaaatat gccatctgaa ggctttagac ggcatatttc
     9661 acgataatag tcgcccaatc aaataaacac agattggaaa tttattttat ttttccctta
     9721 ccaatacccc agaactttcc accaaatgct gccgataacg aaaaaaatca gaaaattgac
     9781 cacgctcatg ataaaacccg ccttccacca ttctcccatt gtggtgtagc ccgaaccgaa
     9841 aatcacaggc gaagtaccgg tcgcataatg agtgagggtc atcataatgt tggatgcggc
     9901 cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt gaaacggcag cagcgaaaaa
     9961 tgcgccgaac atagcggtaa tatgtgcagt agtactggca aacatataat gcgcatacat
    10021 ataagcaagc acgaggatta cgcccgcagc cgtgccgcta acgcccaaac cgccgacact
    10081 ttccgccaac actccggaga accatttaat cagtccgagt ttatttaaaa atgcggccat
    10141 cataatcaat gcgccaaacc aaataatcgt atcccacgcg cttttttctt tcaaaacatc
    10201 gtcccaagtc aatacaccgg aaagcaaaag caggcttaat ccgataaatg cggtggcggt
    10261 ggcgttgata ctaaaagcgt gattgccggt aataagggcg ggaacatctg cccacaacag
    10321 caacaagata ccgaaaatga ccgccataat gatttcgtct gccgacattt tacccatctc
    10381 cctcagacgg tctttggcaa attgaacggc attgggcgtt tctttaattt caggcggata
    10441 caaaaaatat aaaatcaaag gcataacgaa aaaggcgata acgccgggaa cagccattgc
    10501 ccacgcccac gccccccaag aaagacggaa actactgcct aaattttcgg caatcaagtt
    10561 gacgattaaa gggttggggg cagttgcagt aataaacata gccgacgaaa tgggattgga
    10621 atgatagttg accaaagcca aatatttacc catcttgcct tctgtgcctt ttgcgggatt
    10681 ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt ataatgccgc cgccgcgcgc
    10741 ggtattggaa ggggtaacgg gagccagcag cagttcggaa agagcgagac tgtaaccgat
    10801 gcccagcgtt tttcttccaa aaacggcgat aaacaaatat ccgatacgca tccccagccc
    10861 tgttttgagc aaaccgcgcg aaatcataac tgcgatggca atcagccaaa tcaacggatt
    10921 ggcgaacgca ctcaacgcat cgctcatcgc cgcacccggt ttgtcggcgg ttacgccggt
    10981 tactgcgacc aacccgacgg caataatcga cagcgcaccc aacggcatgg ccttgccgat
    11041 aatggcggca atcacaccga taaacatggc cagcagcgtc caagcctgag gcttgacccc
    11101 gtcgggtacg ggcagtgcca aaaccaaggc gcacaatact gcggcaatgg cgaggggtat
    11161 cggtttgaaa cccaatttca tcatattgac ctccgtaaaa aagaccgtcc cgaaaaatcg
    11221 gaaaaataat atttaactaa ttgttttata agatatattc cgatatttca ccgtctttcc
    11281 gatatgcggc tccgggcaac ttttgtttca gtatttgaat tttcattaga ctgaatacgc
    11341 cgtttgaacg gcacggcgaa aacccggggg atgccggacg ttcagtcctt tttcgcacct
    11401 tgaaggtaaa gcatttccaa ggcaattgtg gcaccggcca aagcggtaat gtcggattgg
    11461 tcgtaagagg gggcaacttc tacaacatcc ataccgacga tgtcgagatc cgtcagccca
    11521 cgtaggattt ttaatgccct gtcgctgctc aagccgccgc atacgggcgt accggtccca
    11581 ggggcgaacg acgggtccag gcagtcgatg tcgaaagtca ggtaaacggg catattgccg
    11641 acggtttctt tgattttacg gacggtctct tcaacactgt cttcattgac ttggggggcg
    11701 gtcaacacag taaaaggcaa ttttttactg tgttcggtac gtatgccgat ttgcacggaa
    11761 tgtgccggat cgatgatgcc ttccttgggg gcggtataaa acatcgtacc gtggtcgtat
    11821 tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat gaatcagtgc gagtttgccg
    11881 aaatagcggg cgtgggcgcg caacaacggt agggtaatga aatggtcgcc gcccaaactc
    11941 aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg cttccatttt ttcgacaaaa
    12001 tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat caataatgtt caggcgttcg
    12061 cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag cgaggttgac ggaggcgcgc
    12121 cggatggctt caggaccgaa acgcgcgccg gaacgccctg aaaccgccat atcataaggc
    12181 acgccggtaa taacccaatc ggcatgactt tcatacggca taaaattaag cggcaggcgc
    12241 aaaaacccga aattattgga aacgagggag ttgtcggttt gtcctgccag tgtgctgtat
    12301 tgcatcgtaa tgattccttg taattggttt caatcggtcg tgatgattgg tgtttgagta
    12361 agaaaatcgg gcttcagacg acatatccga tgccttgatg cgtctattcg tcttccaaat
    12421 aggtataacc attaagcccc gcttcgagtt cttttaagaa agacatagcc tgcgaggcag
    12481 gaaggtctga atgttcgatt tgttcgcgat agcgtttcat cagctctttc ggatcttgat
    12541 aaacgtattc gagcatatcg gcaacggtgt ttccttcatc gtaatcgatg acggtaaatt
    12601 gtccgtcttc ccctacaaca acatcggcag tggcagtgtc gccgaaaaga ttgtgcatat
    12661 tgccgagtat ttcctgatat gctcccacca taaaaaagcc taaaagcggc ggctcttctt
    12721 cgggataatc aggcataggc atcgtaccgg cgatgccgtc gccgtcgatg tagtggtcaa
    12781 tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc gcggcgcgca atcggttcat
    12841 tcaaaccggt aatgggacaa acagggaaaa gttgatctat gccccaagca tcgggcaaag
    12901 attggaagag tgagaaattg acatacagct tatcggcaaa acgttcttgc aattcgtcaa
    12961 taatggttcg gtgagaccgg tgtttttcat taaacaattc gccgacttca tgacagatat
    13021 ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact caacagcccg acattatact
    13081 gattatgcac atcggcaaga tcaaactgcc cttcgtgtat ccagctgcgt aaggaacgtt
    13141 tttcccgcga ggcggaaata tccgtccaag tttcccacat actgtgcaac acacgcggtg
    13201 cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg ttcaacgcct ataacattag
    13261 caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct ctcggtaatg attgtcggat
    13321 gcggcagccc gtgttcgaga caagcctggc tgatgcccca tacgactgtg gcggcatatt
    13381 cgttgaggct gtaattaacg gaacaatccg attgtgtgcg gtttccttcg taatccacgc
    13441 caagcccgcc gcctacatca aaacagcgga tatttacccc cagtttgtgc aactcaacat
    13501 aaaaccgagc cgattcgtgt acacctgtgg caacatcacg gatgttccca agctgcgagc
    13561 ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt tttttgtttc aaaatatcga
    13621 ccagttgcaa aacttgggaa gccgacaagc cgaattttga tttttcccca cccgaagact
    13681 gccattttcc cgaaccttgg gaagccagtc tggcgcgcac acccaaacgg ggcttgatgc
    13741 cgagtttttc cgcctcttcc aataccattt gtatttcgga cagcttctca atcaccaaat
    13801 aaacctgatg ccccagtttt tcgcccatca aggcgaaacg gatatattca cggtctttat
    13861 agccgttgca gacgattaat gtttgccggg tgccggcgtg tgccaaaacc gccatcagtt
    13921 cggctttaga accagcttcc aaaccatgcg gttgtccgct tgacataagc gattcgatga
    13981 cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca ataaccgccc ttatagccgc
    14041 actcttcccg tgccgtctga aaggcgcggt tgatgtcgcg gaggcggtgt tcgaggattt
    14101 gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt ttgttgcacg gcttcagtca
    14161 gtttttgcag tgaaacagtt tgattgtgtt gcgagggatt ggggcggacg atgatttcgc
    14221 cggaatcgtc aacatcataa taacctatac cccaatgatt aatgttgcac acttcacgga
    14281 tggtaaggat aggcataata aacctgctcc gtctgtcgtg ttgaaaagga atgattataa
    14341 caaatcagcg tgaaatgtca tttttttaat aagaaaagcc tgcctcatac ctgatgagga
    14401 acaggcaaaa tgccgtctga acgcttcaga cggcattttg gttcagcttg ccatcaaagg
    14461 agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg ggtaagacaa cacgatgtcg
    14521 tattcgcggc cgtctttata ggcttccact tccagcacag gcttacccca atggtcgtcg
    14581 gcatcgacat cgtgaacctg ataaccacgc tgttccaaca ttttcacggc ttttgtgcgg
    14641 ttttgttcaa aatggggatc gccgtaaatc tgacgctcgg cagaatcgcc ggcaaatgcg
    14701 gttgcggcac tcagggaaac aacggcggcc aataacagtt ttttcatttt cagtcctttt
    14761 tttatcggtt gattgaacaa gatgtgtttt tcaataccgc cattaaaaca cagcaaaatt
    14821 aggtttgaat tagagggaag tcaagaagcg taaatgccgt ctgaaaaaaa cagagccgtc
    14881 aaacggctct gttttcctta tgcttctttg gtttcagccg ccttttgacg caggcgcaag
    14941 ctcaattcgc gcaactgctt gtcgtccacg ctgttgggcg cgttggtcag caggcattga
    15001 gcacgttgtg ttttcgggaa ggcaatcacg tcgcggatgg attcggctcc ggtcatcagc
    15061 gttaccagac ggtcgaggcc gaatgcaagg ccgccgtgag gaggcgcacc gaatttcagg
    15121 ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag ggctgatttt cagcgcggca
    15181 aacactttct cttgcacgtc tgcgcggtgg atacggatag agccgccgcc gatttcccag
    15241 ccgttcaata ccatatcgta ggcgcgtgcc aaacaatttg ccgggtcgga aaccatcagg
    15301 tcttcatgac cttcttttgg cgcagtaaac ggatggtgta cggcaacgta gcggtcggct
    15361 tcttcgtcgt attcgaacat tgggaagtcg acaacccaca aaggtttcca ttcgtctgtg
    15421 aaatagccgt tttccgcgcc gtgttccaag ccgactttga tacgcaatgc gccgatggct
    15481 tcgtttacga ctttggtttt gtctgcaccg aagaagataa tatcgccgtt ttgtgcgccg
    15541 gtacgcgcga tgatttcctt cagggcattc tcagacagga attttacgat tggagattgc
    15601 aggccgctgt cttcgccgtt ggagaggttg cccacatcat ttactttgat gtatgccaga
    15661 cctttcgcac cgtagatgcc gacaaatttg gtgtattcgt cgatttcttt gcggctgaat
    15721 ttcgcgccgt tcggtacacg cagagcgact acgcggccgc ctttcatatc tgccgcgcca
    15781 cggaagactt tgaattcttc cgttttcatc aggtcggtca actcggtgaa tttcaagttg
    15841 atacgcatgt ccggtttgtc agagccgtag tagaacatgg cttcagagta aggcatgcgt
    15901 gggaagtcgc ccaagtccac gcccagcgcg tctttgaaga cttgtttcgc cataccttcg
    15961 gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt ccaagtcgat ttgggtaaat
    16021 tcaggctgac ggtcggcacg caggtcttcg tcgcggaagc acttggtgat ttggtagtaa
    16081 cggtcgaaac ccgccaccat caacagttgt ttgaataatt gcggcgattg cggtagcgcg
    16141 aaaaactcgc ccggatgaac gcggctcggc acgaggtagt cgcgcgcgcc ttcaggcgtg
    16201 gagcgggtca gcatcggggt ttcaatgtcg atgaaa
//
LOCUS       DAWHXS010000042        16200 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWHXS010000042 DAWHXS010000000
VERSION     DAWHXS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.042"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WIE22_001050"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WIE22_001050"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ4127148.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WIE22_001051"
     CDS             1160..2290
                     /locus_tag="WIE22_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ4127149.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /locus_tag="WIE22_001052"
     CDS             2361..3212
                     /locus_tag="WIE22_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ4127150.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WIE22_001053"
     CDS             complement(3295..3957)
                     /locus_tag="WIE22_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ4127151.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4052..5845)
                     /locus_tag="WIE22_001054"
     CDS             complement(4052..5845)
                     /locus_tag="WIE22_001054"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ4127152.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRSLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6091..7200
                     /gene="proB"
                     /locus_tag="WIE22_001055"
     CDS             6091..7200
                     /gene="proB"
                     /locus_tag="WIE22_001055"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ4127153.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGSALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7213..8475
                     /locus_tag="WIE22_001056"
     CDS             7213..8475
                     /locus_tag="WIE22_001056"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ4127154.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(8700..11732)
                     /locus_tag="WIE22_001057"
     CDS             complement(8700..11732)
                     /locus_tag="WIE22_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ4127155.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAEFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGSGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            11897..12577
                     /locus_tag="WIE22_001058"
     CDS             11897..12577
                     /locus_tag="WIE22_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ4127156.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            12606..12965
                     /locus_tag="WIE22_001059"
     CDS             12606..12965
                     /locus_tag="WIE22_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ4127157.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(13621..14157)
                     /locus_tag="WIE22_001060"
     CDS             complement(13621..14157)
                     /locus_tag="WIE22_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ4127158.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTTD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(14191..14547)
                     /gene="folB"
                     /locus_tag="WIE22_001061"
     CDS             complement(14191..14547)
                     /gene="folB"
                     /locus_tag="WIE22_001061"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ4127159.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            14603..15205
                     /gene="plsY"
                     /locus_tag="WIE22_001062"
     CDS             14603..15205
                     /gene="plsY"
                     /locus_tag="WIE22_001062"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ4127160.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAVLTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGGSR"
     gene            15282..16127
                     /locus_tag="WIE22_001063"
     CDS             15282..16127
                     /locus_tag="WIE22_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ4127161.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLSEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
BASE COUNT         3613 a         4691 c         4060 g         3836 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcgg aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 aagtttttcg gcgttcaatt cttccgccaa tttgcctgcc accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcaatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctt acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaac cataccccgc tgtccaatag cgtgtactac gcctatttcg aaccctattc
     1501 cgaagaacag cacctcaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatag gcaatcaggt
     1621 cgaaagcgat ttgaaaatct ggattaccgc ccgccagcat ccaggcgaaa ccatggcgga
     1681 atggtttgtc gaaggactgc tcggcaggct gctcgaccat caggatgcaa ccgcgcgcac
     1741 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1801 gggcaacctt cgcaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tttggaaaaa agccccgaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccctttaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcctgtt atgattatcc atccccaatt cgatcccgtc ctcatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgcttg
     2521 acgacttcct cacttggggc attttgggcg taatttttgg tgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagac ctcgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa tttgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagtgc cgttgtcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccctat tatcgccgca atgccgtctg aagccttttt
     4021 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgcct tgcgctttcg
     4081 gtttggcggc actaaattcc agtttgctca aagcggaaag gtaggctttg gcggtggcga
     4141 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgc gccagacgga
     4201 cgctggtttc gccctggctt tccgtacctt gcgtgacggc gttgacggaa taaatctgca
     4261 aagccgcgcc gctttgcgcc acgctttcaa tcgctttgaa aatcgcatcc acggggccgg
     4321 agcctgtggc ggaagcgcgt ttttcttcgc ctttgatgct gaacacgatg tcggcgcgcg
     4381 gctcttcgcc ggtttcggtg ctgatttttt gggagatgaa tttgtagctc tcggcattca
     4441 tgctgcccat ttcgtcggat accagtgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4501 tgtcggcgag ttctttgaag cgggcaaacg cggcgttcag tgcctcttcg ctttccaact
     4561 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4621 tcaaacggtt tgttgcccag ccgaccgatt cggcggacat aatctcgtaa gtttcgcggt
     4681 gtttcagcac cccatcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     4741 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     4801 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     4861 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     4921 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     4981 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5041 gttcgcggaa aaattcttcg gttttgtacg ggattgaata gccgacggta tcgggaatat
     5101 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg aaatcgattt
     5161 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca cgagcgattt
     5221 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5281 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5341 cctcacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag accgttgatt
     5401 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5461 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5521 cctctttggt catagcggcg ccgggcgact gctcgccgtc gcgcagggtg gtatcaaaaa
     5581 taattacgcg gttggtctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5641 aagcattcaa atccagcgac ctgccttgcg cttcagccaa cgcggtcagc ttttgcagtt
     5701 gcgccagggg cagcgacccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     5761 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     5821 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     5881 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaatct
     5941 caataagccg ccatagattt tattaatttt atacattttg tctattttat cgcagacgcg
     6001 gcgtgattgt tgacaattgc ggcagaacag ggcaaattcg gcggcacaag gcggcaaacg
     6061 agacaggaca aaccaacaag gacggaaaca atgaaataca aaagaatcgt atttaaagtc
     6121 ggcacatctt cgattaccca ttcggacggc agtctctcgc gcggcaaaat ccaaaccatc
     6181 acccgccagc ttgccgcatt gcatcatgcg ggacacgagc tggtcttggt gtcttccggc
     6241 gcggttgcgg cagggttcgg tgcgctgggt ttcaaaaaac gtccggtcaa aatcgccgac
     6301 aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg aagaatatac ggcaaacctg
     6361 tcttcagacg gcatcgtgtc cgcacaaatc ctgctcagcc gcgccgactt tgccgacaaa
     6421 cgccgctacc aaaatgccgg cagcgcactt tccgtgctgc tgcaacgccg cgccgtcccc
     6481 atcatcaatg aaaacgatac ggtttcggtt gaggaattga aaatcggcga caacgacaca
     6541 ttgagtgcgc aagtggcggc gatgatacag gcagacctct tggtgctgct gaccgacata
     6601 gacggtcttt acacgggcaa cccgaacagc aatcccgatg ccgtacggct ggacaaaatc
     6661 gaacacatca accatgaaat catcgaaatg gcgggcggct cgggttcggc aaacggcaca
     6721 ggcggtatgc tgactaaaat caaagcggcg acgattgcga ccgagtccgg cgtaccggtc
     6781 tatatctgtt cctcgctcaa acccgatgca cttgccgaag cggcagataa tcaggcggac
     6841 ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacgc agaagcaatg gctggcgttc
     6901 tattccgaaa gccggggcgg cgtttatgtg gacgaaggtg cggaacacgc tttgtccgaa
     6961 cagggaaaaa gcctgctgat gtcgggcatt gccggaatcg aagggcattt ttcccgtatg
     7021 gacaccgtaa ccgtgtacag caaggcaacc aaacagcccc tgggcaaagg gcgcgtcctg
     7081 ttcggttctg ccgccgccga agacctgctc aaatcgcgta aggcgaaagg cgtgttcatc
     7141 catcgggacg actggatttc catcacgccc gaaatacgcc tgcttctgac cgaattttag
     7201 aaaaggaaac ccatgtcaaa cacacaaaaa cagcttgccc ttgccaaagc ggcaaaaaaa
     7261 tccgtcaaca cggcggatac ggaagaaaaa aaccgcgcgc tgtttgctat ggcggacagc
     7321 ttggaagcgg cggcggcaga tattttggcg gcaaaccgtc aagacttgga agccgcggca
     7381 ggcaacattc ccgaaagcat gaccgaccgc cttttgttgg acggcaaacg catttgcgcg
     7441 atggcggacg gcatcagggc ggttgccgcg ctgcccgacc ccgtgggcga aatactggaa
     7501 acctcgactt tgcccaacgg cttggaaatc gtcaaaaagc gtgtggcgat gggcgttatc
     7561 ggcattattt acgaaagccg cccgaacgtt acgtccgatg cggcggcttt ggcactgaaa
     7621 agcggcagcg cggtcgtact ccgcagcggc aaagatgcat tccaatccgc acgcgccatc
     7681 gttgccgccc tgaaaacggg gttggcacaa acccgcatcg accccgacgc gctccagttg
     7741 attgaagaca ccgggcgcga aagcagttac gaaatgatga gggcgaaaga ttatctagac
     7801 ctgctgattc cgcgcggcgg ggcggggctg atacgggcgg tggttgaaaa tgccgtcgtg
     7861 ccggtcattg aaacgggaac gggcatcgtc cacatttata tcgacaagga cgcggattgg
     7921 gacaaggcgc tccgtatcgt gtacaacgcc aaaaccagcc gcccgtccgt gtgcaactcg
     7981 atggaagtgc tgctggtgca tgaagacatt gctgccgact tcctgcccaa gctcgaacgg
     8041 ctgttggttc gcgaccgcat agaagccgga ctgccgcccg tccgcttccg tttggatccg
     8101 caggcggcgc ggcatatcgg cggcgaagcg gcgggtgcag acgatttcga taccgagttt
     8161 ttagactaca tcctcgctgt gaaaaccgtc gcatcggtcg aagaggcggt cgggcacatc
     8221 gaagcccacg gcacacacca ttcagacggc atcgttaccg aaaaccgcca cgctgcggac
     8281 tatttcacga cccatatcga ttctgccgcc gtgtatgtca acgcgtccac gcgctttacc
     8341 gacggcggcg aattcggctt gggttgcgaa atgggcatct ccacgcaaaa actccacgcc
     8401 cggggcccga tgggtttgaa agagctgacg agttacaaat acatcgtaca aggcacggga
     8461 caggttaggg aataatccga attcaaacgt ctgatatagt ggattaactt taaaccgata
     8521 cggcattgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg
     8581 aatcggtttc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
     8641 atccactata aaaatgccgt ctgaaacagt cttcagacgg catttccatt tgcaaaaaat
     8701 caagcattgt ccaaggggac gagaatcgta cggttgccgt tgtgttccgg tgcggacaca
     8761 atgccttccg cctccatctg gtcaatcaga cgcgcggcgc ggttgtagcc gatacgcaag
     8821 gcgcgctgta cgcccgaaat gctggctttg cgcgttttca ggacaacgga tacggcttca
     8881 tcatacatcg gatcggtttc gtcgtcgccg ctgcgcccga tgccgggcag ctcttcgctg
     8941 ccgctgccgc tcaaaatatc gtcaacatag tccggctcgc caaactgttt caaatattcg
     9001 accacgcggt gcacctcttc atccgaggca aacgcgccgt gaacgcgctg cggataggcc
     9061 gtacccggcg gcaggaacag catatcgccc tgaccgagca ggttttccgc gcccatttgg
     9121 tcgagaatcg tgcggctgtc gattttgctg gatacttgaa acgcgatacg cgtcgggatg
     9181 tttgccttaa tcagacccgt gatgacatcg acgctggggc gttgcgtggc aagaatcaaa
     9241 tggatgcctg ccgcgcgggc tttttgggcg aggcgggcaa tcagttcttc gattttcttg
     9301 cctgccgtca tcatcaggtc ggcaaactca tcgaccacga ccacgataaa cggcagtttt
     9361 tccaaaggtt cgggatcgtc gggcgtgagg ctgaacggat tgccgatttt ttctcccctt
     9421 gctgcggctt cggcgatttt ttgattgaag cccgcaagat tacgcacgcc cataaagctc
     9481 atcaggcggt agcgtttttc catttcgtta acacaccagt tcagcgcgtt tgccgccagc
     9541 ttcatatcgg taacgacagg ggcgagcagg tgcgggatgc cttcgtaaat gctcaattcc
     9601 agcattttcg gatcgatcat aatcatacgc acgtcttccg gcgcggcttt gaaaagcata
     9661 gacagaatca tcgcgttgac acccaccgat ttgcccgaac cggtcgtgcc ggcaaccagc
     9721 aaatgcggtg ctttgcccaa gtcggttacg acgggctgtc cggtgatgtc ctgaccgagc
     9781 gcgagcgtca gcttggattt ggattcggca aactcgggcg aattgaagat ttcgctcagg
     9841 cgtatcattt ggcgtttcgg gttcggaagt tccaaaccca tacaggtttt gccggggatg
     9901 gtttcgacaa cgcggatgga agccacgccg agcgaacgcg ccaagtcttt ttccagattc
     9961 agaacggaat tgccgcgcac gccgacatcg ggttcgattt cataacgcgt aatcacgggg
    10021 ccggaataag aatcgacaac cttgaccttg actttgaact ccgccaattt ttcttcgatg
    10081 gtgatgctgt tttccaacag ttcttcttcg gtttgcgtcg cctcgggatt gaacagcggc
    10141 ggcagaagca ggtcggttgt cggcaggtgt tcggataccg caccggtctc ttccgattgg
    10201 aacgcccctt catccgcttc cgtatccgat acccggcagg acgggcgttc agacggcaca
    10261 ttttcaaacg gacaaaccgc ctgactgtca tgcccgaacg cttcggtttc cgacagatat
    10321 tgcccgcttg accgctctgc cgcaccttca ctgccgtcat ctgcaatagc ggcggtttcc
    10381 tcctgccaac ctccattcaa aacatcatcg gcaagatggt cggtttcggc aatgcggctg
    10441 cgttgcgcct gctcgaattc tgccggcggt tcataggtac ggttgtagat ttccgatacg
    10501 ggaggcggcg gcggaatatc gattgcgggc atgggaactt tcggcacttc gggtggttcg
    10561 accacccaag catcgggcgc agcaggttct tcaatataga tatcggcagc ttcatcataa
    10621 accggaccgc cgtatcccac agacgaaacg gtttccgtga aaacattttc ggtttcagac
    10681 ggcatttcga catcccgttt cccgacaacc gtccgacttt ccggaatcag gcgcgaaatc
    10741 cgcgcctccg cagaaaccgt tgccttgttc ggttctttca aattgacgga aacgcggcgg
    10801 caggcatcgc ggacggctgt gccgtctgaa acggactgtc cttgggaaac atccggggat
    10861 tttgcctccg cctgccctgc atgacggcgg aacggcgcat tccgtgcatt ttcggctttg
    10921 actgcagaaa aggcagacgg atgctgcttc tcaaaatacg gctcaaatcc gtaatccgca
    10981 gactcggaaa acgcttcttt gtccgcatcg aacatacggg aatacgtgcg ttgaagcaca
    11041 gggtcgtcca aaccgataat ctgcagccct tccatcgggg tatcggacac gcggactttg
    11101 gggaccgctg tttcgttttt ctcaaatgca tcgatatagc gttttttcgt ttcccttaaa
    11161 gccgcgccgt ttaaggcacg cgttgcttct tccaaagtga tttccttaaa aaccggacgg
    11221 acgggcgaaa tttcgctttc agacggcatc aacccttcag caatactccg gtcgaatggg
    11281 atgcggcggt tgtcgattac ggcagttgca atgtcttccg tatcggcagc ctcttcctcc
    11341 gcagcttctg ctgcttccgt ttccgcttct tccgtcccgt ttccactgtc tgaatatccg
    11401 tcggatgcgt tcggaacatc cgcacggttt gcagaagcag gcggaacagg cctatccggt
    11461 tcggattcgt gcgccagata gtttcggcag aaacggacga caccgacgaa cagccaatac
    11521 actgccgttt tgacggcatg gaaaagcatc agggcaagtt cgggaaaatc gggcataccg
    11581 tctttgattt caggcagctc cgcctgtttt tcccctttct tttcctgcca cgcagaaacc
    11641 tcgcgcatcc actcgcgttc ggattgtgcg cggacaaaaa acaagccggc aagcgcaagc
    11701 aacaaaataa cgatcataac tatccaaaac atatgccgtt tcccgcaaga caaacgatta
    11761 aacgggttta ttttaacgtg tttccatccc aaaaaaaaca accggtcggc aattctgccg
    11821 aaaccgattc ggctttcggt ttgcccccac cgacagtata atagccgtcc cttcccttaa
    11881 tctttgcggc attgccatga ttttccaaac agtctggttt tcagatgtgg tactgtcggt
    11941 ttcgtggatt gtccttatcc tgatactggc agcttccgct ccgtcggcat tccgctcgct
    12001 tgcccgctac cgttctgccc ttcccctgtg taccgtaatt ttctccgcgg catggtgtct
    12061 gaacgcctct gccggcggcg gacagcttgc acagatgaac taccacctgc tcgccgtcaa
    12121 tctagttgct ctgatggtgg gcacttccgc cgccttttgg cttgccgcgc tgctgatgct
    12181 gccctactgc ctgctgtttg ccggttctgt cggcgcatat ccgccgaacg ctttggtgct
    12241 gattctgccc gcgctggccg tcaaccgcct gtcgcgtatg ctggtcaacc gcctgccacc
    12301 caatattttc atctttattt tcgtcaacgg ctttctcgct tccgccgccg gcattctgct
    12361 gaccgggctg gtgctgaccg gtattttgga tgccgcaaac gcttttccgt ccgaaatatt
    12421 gtggacgacc gccctgcccg tctttattct gctggcgtgg gcggaagctt ttctcagcgg
    12481 catttcaact gccatatttg ttgcactgaa gccccattgg atcaacactt tcgacgacaa
    12541 ccgctatctg aagtccgacc gcggcatatg gcggtagcct tcagacggca tagacaggca
    12601 aaaccatgct ttccaacatc atcccccttt ccatcggtgc ggcactcggt gcaacggcgc
    12661 gttggctgct caacctcgcc gttcctgccg ccatgtctcc cgccacgggc aacctgtttg
    12721 ccaactggac gggcgcgctg ctcatcggca tctttgccga aaccatcaat cacccgcaat
    12781 ggaagctgct gctgattacc ggttttctcg gcagcctgac cacgctttcc ggattttcac
    12841 tggaaaccgt aaccctgctc caatcgaatc gtcctgcttc agcacttgcc aatattttcc
    12901 tacacacggc aggttcgctg ctgctgactt ggctcgggct gaagataggg gcggcagtca
    12961 aataaccgca acaaattgcc tccctttgtt ttgacataat ctataaatta gcttcgcttg
    13021 tttaaatact ggctgtgcca attatagtgg attaacaaaa atcaggacaa ggcgacgaag
    13081 ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac cttagagaat
    13141 cgttctcttg gagctaaggc gaggcaacgc catactggtt tttgttaatc cactataccg
    13201 ttctcaaatt caaattgggc ttttaggtct ataacaaaga aaaagccttg gcaggataaa
    13261 cctaccaagg ctttttatta aaaaaaacgc tctattactg tgtctaaaaa tgcaaaaaaa
    13321 gtaaccatca taaccctttt gccaaaatca atatcatctt ccgatgaatg aattgacggt
    13381 atataagaaa gaattaataa gctcataaac cggtaagaaa aatcagtaac aaagattttc
    13441 attattaacc attcttaaaa atgatcacgt tcgtttgggt aattattata cccttttccc
    13501 tcttattact aaagcacgaa tgactttaaa ggacgcgccc agagacaagc cgaaaaggca
    13561 tatctcatcc gccaaacaaa atgcccgcca atctactccg gagggcagtt caacaccctg
    13621 tcaatccttc atcagccagt attgcaaacc gattaatgtc ttgccgtctt taatttcatc
    13681 gtttgccaat gcctgacgga cttcttcttt cgacatcaat acggtttccg taatctcgtc
    13741 ttcgtcattg gcaagcgtac tgcccaaacg cacgccttcc gcctcgaaca gatacatttt
    13801 ttcgttgcaa aaaccgaccg ccgtataaaa actgtaaagc aggcgtacgc tgtcggtggt
    13861 ataaggcgtt tcttcagcca gttctcgtaa agcgcatgct gccatatcct cgcccgccac
    13921 atccagcttg cccgcaggaa gttccaatgt cgcctgattt gccgcatacc gccactgccg
    13981 taccaaaacg accttttcct catccgttac cgccaacacg cacgccgcgc ccggatgccg
    14041 gatgacgata cgctgccctt cattgccgtt gggcaacctg accttatccc tgctgatgct
    14101 gacgaaaccg ccctcgtaaa tggtttcgcc gcctaatttt acttccctca aatccacttc
    14161 atatctcctt tccgtttcct attctgccgt tcaatcttca cgcttgccgc gctcgatttc
    14221 cacgccaacc tcgcgcacgc cttccaaaat acccggcttg acgattttca cgcgcaccca
    14281 caccgcgccg aaatatccca aaaccaaatc ggcaatatat tccgccaacg cttccaaaag
    14341 caggaaatcc tgttctttca gatgtcggcg cagcgtttcg catacctcgg cataatgcac
    14401 cgtattggca atatcgtcgt ccgaacccgc tttctcggga acgccgatgt ccaaatcgac
    14461 aatcagggtc tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg tatccgcctt
    14521 gatgccgtgc aaaaagattt tatccataat ccgccccgtt ttttgctaaa ataaccgcac
    14581 cattctaagt aaagtaccgc aaatgttcaa cataccggct gttgctgttt cctatctgat
    14641 cggctcactt tctttcgccg tcatcgtgtc caagtattac ggcatggacg acccgcgcac
    14701 ctacggatcg ggcaatccgg gggcaaccaa tgttttacgc agcggcaaaa aaaaggcggc
    14761 cgtgctgacg ctcttgggcg atgccgccaa aggtttggtt gccgttttgc ttgcacgcgt
    14821 gcttcaagaa ccgctcggtt tatccgacag cgcaatcgct gccgtcgcac tcgccgcgct
    14881 ggtcggacat atgtggccgg tgtttttcgg atttaaaggc ggcaaaggcg tggcaacggc
    14941 attgggcgtg cttctggcac tctctcccgc aactgccttg gtctgcgcgt tgatttggct
    15001 tgtgatggca ttcggcttca aggtgtcctc ccttgccgca ttaaccgcca caatcgccgc
    15061 cccccttgcc gcactgtttt ttatgccgca tacttcttgg attttcgcaa ccctcgcaat
    15121 cgccatattg gtgttgctcc gccataagag caacatcctc aacctgatta aaggcaaaga
    15181 aagcaaaatc ggcggcagcc gctgatttcc catccatccc tgtttatgcc gtctgaaccc
    15241 cgcttcagac ggcatccgaa tatcccgccg ttttaccgac tatgctcagt tacagacacg
    15301 cattccacgc cggcaaccac gccgatatgc tcaaacactt caccttgttt ttagtgctgc
    15361 aatatttcaa ccgcaaagac aagccctact ggtacatcga cacgcacggc ggcgcgggtg
    15421 tgtacaatct cgaaggcagc gaggcacaaa aagtcggcga ataccggcaa ggcatcgccc
    15481 tacttcgaca agcaaaaaac ctgccaaccg agttaaacgg ctttatcgcg cacatacaaa
    15541 aaatcctgcc ctcgcccgaa ctttactgcg gttctccgtg gctggcgcaa tcgctgaccc
    15601 gcgtcggcga caaattacgc ctgtttgagc tgcaccccac cgactttgtc catctgcaaa
    15661 acaatatggg cgaagcagga ttgggcaaac gcggacaagt gttgcgcgaa gacggttaca
    15721 aagggctgat ttccctgctg ccgcccccgc cgcgccgcgc caccgtcctc atcgacccgc
    15781 cctatgagga aaaacaggac taccggcgtg taacggaaac gctgaaagcc gctttaaaac
    15841 ggtttgagtc cggctgttat ctcatttggt atccctgcct cagccgcgaa gaaagccgca
    15901 agctgcccga agaattgaaa aaactcctgc ccgacaacta ccttcacgcc gaacttcacg
    15961 tccacgcgcc gaaagccgac ggtttcggta tgcacggcag cggaatgttc gtcatcaatc
    16021 cgccctatct gttgtcggaa caattggcgg caaacctccc cgccctgacg cggctgctgg
    16081 cgcaagacga aggcgcgcgc taccttttgg acagcaaaat ccgctgaccc cgatgccgtc
    16141 tgaaaccctt cagacggcat ctcccatcaa acaaaaccgg acgatatacc ggcagccggc
//
LOCUS       DAWHXS010000043        16187 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWHXS010000043 DAWHXS010000000
VERSION     DAWHXS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16187
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.043"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            49..124
                     /locus_tag="WIE22_001064"
     tRNA            49..124
                     /locus_tag="WIE22_001064"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Trp,seq:cca)
     gene            239..517
                     /gene="secE"
                     /locus_tag="WIE22_001065"
     CDS             239..517
                     /gene="secE"
                     /locus_tag="WIE22_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ4127162.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            519..1055
                     /gene="nusG"
                     /locus_tag="WIE22_001066"
     CDS             519..1055
                     /gene="nusG"
                     /locus_tag="WIE22_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ4127163.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1156..1590
                     /gene="rplK"
                     /locus_tag="WIE22_001067"
     CDS             1156..1590
                     /gene="rplK"
                     /locus_tag="WIE22_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ4127164.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1590..2285
                     /gene="rplA"
                     /locus_tag="WIE22_001068"
     CDS             1590..2285
                     /gene="rplA"
                     /locus_tag="WIE22_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ4127165.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2515..3015
                     /gene="rplJ"
                     /locus_tag="WIE22_001069"
     CDS             2515..3015
                     /gene="rplJ"
                     /locus_tag="WIE22_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ4127166.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3073..3444
                     /gene="rplL"
                     /locus_tag="WIE22_001070"
     CDS             3073..3444
                     /gene="rplL"
                     /locus_tag="WIE22_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ4127167.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3635..7813
                     /gene="rpoB"
                     /locus_tag="WIE22_001071"
     CDS             3635..7813
                     /gene="rpoB"
                     /locus_tag="WIE22_001071"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ4127168.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDKDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7970..12145
                     /gene="rpoC"
                     /locus_tag="WIE22_001072"
     CDS             7970..12145
                     /gene="rpoC"
                     /locus_tag="WIE22_001072"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ4127169.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVNQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGAAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12437..12694
                     /locus_tag="WIE22_001073"
     CDS             12437..12694
                     /locus_tag="WIE22_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127170.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGNRMGMPINIG"
     gene            12691..12840
                     /locus_tag="WIE22_001074"
     CDS             12691..12840
                     /locus_tag="WIE22_001074"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4127171.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13019..13390
                     /gene="rpsL"
                     /locus_tag="WIE22_001075"
     CDS             13019..13390
                     /gene="rpsL"
                     /locus_tag="WIE22_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ4127172.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13508..13978
                     /gene="rpsG"
                     /locus_tag="WIE22_001076"
     CDS             13508..13978
                     /gene="rpsG"
                     /locus_tag="WIE22_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ4127173.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13997..16102
                     /gene="fusA"
                     /locus_tag="WIE22_001077"
     CDS             13997..16102
                     /gene="fusA"
                     /locus_tag="WIE22_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ4127174.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4301 a         3591 c         4190 g         4105 t
ORIGIN      
        1 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aagtttagag gccaatagct
       61 caattggtag agtatcggtc tccaaaaccg agggttgggg gttcgagacc ctcttggcct
      121 gccaaataaa aaattaaccg gccttgtgtc ggttaatttt tttgtatttg ttatttagta
      181 aactctcttg ccatttacat ggattgagaa tagacagatg ctatgatgga taaataatat
      241 gacagaacat acgcctgaaa aaaagaacgt taaagtggat caactggttg ttcaagataa
      301 agaatctgca tctaattccg gtaaggaagg gttttttgca tatttctcaa attcttggtc
      361 cgaattcaaa aaggtggttt ggcctaagcg tgaagatgct gtcagaatga ctgtatttgt
      421 tatagtgttt gttgctgtgc tttctatatt tatctatgcg gcagatacag caatttcgtg
      481 gttatttttt gatgtattgc tgagaaggga aggttgagat gtcgaaaaaa tggtatgttg
      541 tacaggcgta ttcggggttt gagaagaatg tccaacgaat attggaagag cgcattgccc
      601 gtgaggagat gggagattat ttcggacaaa ttctggtgcc tgtagagaaa gttgttgata
      661 tccgcaatgg tcgtaagact attagtgaaa gaaagtcata tcctggttat gtgctagttg
      721 agatggaaat gacagatgac tcttggcatc ttgtaaaaag caccccccgt gtttccggtt
      781 ttattggagg gagggctaat agacctacgc cgattagtca gagagaggct gaaattattt
      841 tacagcaggt tcagaccggc atagagaagc cgaaaccaaa agttgaattt gaggtcggtc
      901 aacaggttcg tgtaaatgaa gggccgtttg cggattttaa cggggtggtt gaggaggtca
      961 attatgaacg gaataagtta cgcgtgtctg ttcagatatt tggtagagaa acacccgttg
     1021 agctggagtt cagccaggtt gaaaagatta actgattttt atacttgaaa aaaaagcaat
     1081 aagaggatag aatcaaaaat taacttgggg agcggaaatg gttccgcgtc ttacccgttt
     1141 ttaggagttc gttaagtggc aaagaaaatt atcggctata ttaaactgca aattcctgca
     1201 ggtaaagcca atccatctcc tccggttggt cctgctttgg gtcagcgcgg tttgaatatt
     1261 atggaatttt gtaaggcatt taatgctgca acccaaggta tggagcctgg cttaccgatt
     1321 ccggttgtga ttactgcatt tgcagataaa tcattcacat ttgtgatgaa aaccccgcca
     1381 gcttctatct tgttgaaaaa ggctgccggt ttgcaaaaag gtagttctaa tcctctgacc
     1441 aacaaagtgg gtaaattgac ccgtgcccag ttggaagaaa ttgctaaaac taaagatcct
     1501 gatttgactg ctgctgactt ggatgcggct gtccgtacta tagcaggttc tgctcgctca
     1561 atgggcttgg atgtggaggg tgttgtataa tggctaaagt atctaaacgc ttgaaagctc
     1621 ttcgctcttc tgtggaagcc aataaattat atgcaattga tgaagcaatt gctttggtaa
     1681 aaaaagcagc gactgctaaa tttgacgagt ctgttgacgt atctttcaac ttgggcgttg
     1741 atccgcgtaa atctgaccaa gttatccgtg gttcggtcgt tctgcctaaa ggcaccggta
     1801 agacaacccg tgtggctgta tttactcaag gtgcaaatgc agaagctgct aaagaagctg
     1861 gtgcagatat cgtcggtttc gaagatttgg ctgctgaaat caaagcaggc aatctgaact
     1921 ttgacgttgt tattgcttct cccgatgcaa tgcgtattgt tggtcaattg ggtaccatct
     1981 tgggccctcg tggtctgatg ccaaacccta aagtaggtac ggttacccct aatgttgccg
     2041 aagcagtaaa aaatgcaaaa gcaggtcaag tgcagtaccg taccgacaag gcaggtattg
     2101 ttcatgcaac gattggtcgt gcttcattcg ctgaagctga tttgaaagag aactttgatg
     2161 cgttgctgga tgctatcgtt aaagccaagc ctgctgccgc taaaggtcag tatctgaaaa
     2221 aagttgctgt gtctagcacc atgggtttgg gtattcgcgt tgatacatca agcgtaaata
     2281 actaatctta aggaattttc aagcagtttg gttttctggg ctgcttgaat ttgggctact
     2341 taaaattaag tagatgtcca agaccgtagg gatcgtaaga tttaatcgta actgccctac
     2401 gcagacggta gtcctgaaac acattgcaag attgcttgta agatgtcttt ttaggttacc
     2461 gcgctggtgg gatatcgttt tggtatcctg tttataaaca gtgggaggta gaccttgagt
     2521 ctcaatattg aaaccaagaa agtggcggtc gaggaaatta gcgcggcaat tgctaatgct
     2581 caaaccctcg tagtcgctga atatcgcggt atcagtgttt ccagtatgac tgagcttcgt
     2641 gcgaatgcac gtaaagaagg cgtttatttg cgcgttctga aaaatacttt ggctcgtcgt
     2701 gcagtgcaag gtacttcatt tgcagaattg gccgatcaaa tggttggtcc gttggtttac
     2761 gctgcttctg aagatgctgt tgctgctgct aaagtgttgc accaattcgc gaaaaaagat
     2821 gacaaaattg tcgttaaagc cggttcttac aatggcgaag taatgaatgc tgctcaggtt
     2881 gctgagttgg cttctattcc gagccgcgaa gagctgttgt ccaaactgtt gttcgttatg
     2941 caagctcctg tatcgggctt tgcgcgcggt ttggctgctt tggcagagaa aaaagccggc
     3001 gaagaagccg cttaatcgat tttgtttctg ttaatcaatt attttttaat acaatatttg
     3061 gagtaaaata gcatggctat tactaaagaa gacattttgg aagcagttgg ttctttgacc
     3121 gtaatggaat tgaacgactt ggttaaagct tttgaagaaa aattcggtgt ttctgctgct
     3181 gctgttgcag ttgcaggtcc tgctggtgcc ggtgctgccg atgctgaaga aaaaaccgaa
     3241 tttgatgtcg ttttggcttc tgccggcgat caaaaagtcg gcgtgattaa agttgtccgt
     3301 gcaattaccg gtttgggtct gaaagaagct aaagacatcg ttgacggtgc acctaaaacc
     3361 attaaagagg gtgtttctaa agctgaagcc gaagacatcc aaaaacaact ggaagaagcc
     3421 ggcgctaaag tcgaaatcaa ataatttgat gcttcttatg aaggctggca gttttctgcc
     3481 agccttattt tgcttcttaa aataagcatt aagtattgtt tacatttatt tgcatagttt
     3541 ttatcaaatc attgcaaata aatgtaaaca tcagattgat gcgtgccgtt gctttcagac
     3601 ggcctattat tgaaaattac ttttcggagt gtatatgagc tattcgttta ccgagaaaaa
     3661 acgtatccgt aagagttttg caaagcggga aaatgttttg gaagttcctt tcctgctggc
     3721 aacccaaatt gattcttatg cgaagttttt gcagctggaa aatgcttttg acaaacgtac
     3781 cgatgacggt ctgcaggcgg catttaattc tattttcccg attgtgagcc ataacggtta
     3841 tgcgcgattg gagtttgtgc attacacatt gggcgagcct ttgttcgata ttcccgaatg
     3901 tcagttgcgc ggaatcactt atgcagcccc cttgcgcgcg cgtatccgtt tggtgatttt
     3961 ggataaggaa gcatctaaac cgacggtaaa agaagttcgt gaaaacgaag tgtatatggg
     4021 cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt aacggcacag agcgtgtgat
     4081 tgtctcccag ttgcaccgtt cgcccggtgt attcttcgag catgacaaag gtaagacgca
     4141 ctcttccggc aaattgttat tctccgcccg catcattccc taccgtggtt catggttgga
     4201 ttttgaattt gatccgaaag atttgctgta tttccgtatc gaccgccgcc gtaaaatgcc
     4261 ggtaacgatt ttgttgaagg ctttgggcta caacaatgag caaatcttgg atattttcta
     4321 cgacaaagaa acgttctatt tgtcttcaaa cggtgttcaa accgatttgg tcgcagaccg
     4381 tctgaaaggc gaaactgcca aggtcgatat cttggataaa gaaggcaatg tattggttgc
     4441 caaaggtaag cgcattactg cgaaaaatat ccgtgatatt accaatgcag gcctgacccg
     4501 tttggatgta gaaccggaaa gcctgctggg caaagcattg gctgccgatc tgattgattc
     4561 ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt acagaagagt tgttggccaa
     4621 atttgatatc aacggcgtaa aagaaattac gaccctttat atcaatgagc tggatcaggg
     4681 tgcttatatc tccaatacct tgcgtacgga tgagactgcc ggccggcagg cggctcgtgt
     4741 tgcgatttac cgtatgatgc gtccgggcga accgcccacc gaagaggcgg tcgagcaatt
     4801 gtttaaccgc ttgttcttca gtgaagacag ctacgatctg tcccgcgtag gccgtatgaa
     4861 atttaatacg cgcacatacg aacaaaaact gtccgaagcc caacaaaact cttggtacgg
     4921 ccgcctgctg aacgaaacgt ttgccggtgc tgccgacaaa ggcggttatg tcctgagcgt
     4981 cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg cgtaacggcc atggcgaagt
     5041 ggacgatatc gatcacttgg gcaaccgccg agtacgttcg gtaggcgagc tgactgaaaa
     5101 ccaattccgt agcggtttgg cccgtgtgga acgtgccgta aaagaacgtt tgaatcaggc
     5161 ggaatcagaa aacttgatgc cgcacgattt gattaatgca aaacctgttt ctgccgctat
     5221 taaagaattc ttcggctcca gccaattgag tcagtttatg gatcagacca accccttgtc
     5281 tgaagtaacc cataaacgcc gtgtatctgc attgggtccg ggcggtttga cccgcgaacg
     5341 tgcaggattt gaggtgcggg acgtgcatcc gacccactac ggtcgcgtat gtccgattga
     5401 aacgcctgaa ggtccgaaca tcggtttgat caactcattg tccgtttatg cgcgcaccaa
     5461 tgattacggt ttcttggaaa cgccttaccg ccgcgttatc gacggcaaag taactgaaga
     5521 aatcgactac ttgtctgcca tcgaagaagg ccgctatgtg attgcacagg cgaatgccga
     5581 tttggacaaa gacggcaatc tgattggcga cttggttact tgtcgtgaaa aaggcgaaac
     5641 cattatggca acgcctgacc gcgttcaata tatggacgtg gcaaccggtc aagtggtatc
     5701 cgttgcagca tccctgattc cgttcttgga acacgatgac gcgaaccgcg cattgatggg
     5761 tgcgaatatg caacgtcagg cagtgccttg cttgcgtcct gaaaaaccga tggtcggtac
     5821 cggtatcgag cgttccgttg ccgttgactc tgctactgca atcgttgccc gccgaggcgg
     5881 cgtggtcgag tatgtcgatg ccaaccgtgt tgtgattcgt gtccatgacg acgaagcgac
     5941 tgccggcgaa gtgggtgtcg atatttacaa cttggttaaa tttacccgtt ccaaccagtc
     6001 taccaatatc aatcagcgtc ctgccgtcaa agccggcgat gttttgcaac gcggcgattt
     6061 ggtggccgat ggcgcgtcca ccgatttggg cgaattggct ttgggtcaaa atatgaccat
     6121 cgccttcatg ccgtggaacg gttacaacta cgaagactcg attctgattt ccgaaaaagt
     6181 ggctgcggac gaccgctata cttcgattca cattgaggaa ttgaatgtcg ttgcccgcga
     6241 taccaagctg ggtgcggaag acattacccg cgatattccg aacttgtccg agcgtatgca
     6301 aaaccgtttg gacgaatccg gtatcgttta catcggtgcg gaagtagaag ccggcgatgt
     6361 gttggtaggc aaggtaacgc ctaaaggcga aacccagctg acgccggaag aaaaactgct
     6421 gcgcgccatc ttcggtgaaa aagcatctga cgtaaaagat acttcattgc gtatgcctac
     6481 cggcatgagc ggtaccgtta tcgacgttca agtcttcact cgtgaaggta ttcaacgcga
     6541 caaacgtgct caatccatta tcgattccga gttgaaacgc taccgtttgg atttgaacga
     6601 ccaattgcgt attttcgaca acgacgcatt cgaccgtatc gagcgtatga ttgtcggtca
     6661 gaaagccaac ggcggcccga tgaagctggc caaaggcagt gaaatcacga ccgaatatct
     6721 ggcgggtctg ccgagcaggc acgattggtt cgatatccgt ctgaccgatg aagatttggc
     6781 caagcagttg gaactgatta aagtgagcct gcaacaaaaa cgcgaagaag cggacgagtt
     6841 atacgaaatc aagaagaaaa aactgaccca aggcgacgaa ttgcaacccg gcgtacaaaa
     6901 aatggtgaaa gtttttatcg ccatcaaacg ccgtctgcaa gccggcgaca aaatggcggg
     6961 ccgccacggt aacaaaggcg tggtatcgcg cattctgcca gtggaagaca tgccttacat
     7021 ggcggacggc cgtccggtag acatcgtact gaacccattg ggcgtacctt cccgtatgaa
     7081 catcggtcag attttggaag ttcacttggg ttgggcagca aaaggtatcg gcgagcgtat
     7141 cgaccgtatg ctgaaagagc aacgcaaagc aggcgagttg cgcgagttct tgaacaaact
     7201 ctacaacggc agcggcaaga aagaagattt ggatgccctg actgatgaag aaatcatcga
     7261 actggcctcc aacctgcgca aaggtgcatc tttcgcctct cctgtattcg acggtgcgaa
     7321 agagtctgaa atccgcgaaa tgttgaactt ggcttatccg agcgacgatc ctgaggttga
     7381 aaaactgggc ttcaacgaca gtaaaaccca aatcacgctg tatgacggcc gttcaggcga
     7441 agcatttgac cgcaaggtta cagtaggtgt gatgcactat ctgaaactgc accacttggt
     7501 tgacgaaaaa atgcacgcgc gttctaccgg tccgtacagt ctggttaccc agcagccttt
     7561 gggcggtaaa gcccagttcg gcggccaacg tttcggtgag atggaggttt gggcactgga
     7621 agcatacggc gcagcctaca cgctgcaaga gatgctgact gtgaagtctg acgacgtgaa
     7681 cggccgtacc aaaatgtacg aaaacatcgt caaaggcgaa cacaaaatcg atgccggtat
     7741 gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca ctgggcttgg atatcgattt
     7801 ggaacgttac taaacaaaag ttttcagacg gcctttcagg gtcgtctgaa aaagtggttt
     7861 cagaataaga atgaagcaat cggcatttag gccgtctgaa atcaaaagta ccgtttcccc
     7921 atatcgaaaa tccgccatgc ggtaaaaata cttccttcaa ggagcaaaaa tgaatttgtt
     7981 gaacttattt aatccgttgc aaactgccgg catggaagaa gagtttgatg ccattaaaat
     8041 cggtattgcc tctcccgaaa ccatccgctc atggtcttat ggcgaagtta aaaaacctga
     8101 aaccatcaac taccgtacgt tcaaacctga gcgtgacggt ttgttctgtg ccaaaatctt
     8161 tggcccggtc aaagactacg aatgcttgtg cggaaaatac aaacgcttga aatttaaagg
     8221 tgtaacttgt gaaaaatgcg gcgtggaagt aaccctgtcc aaagtgcgcc gcgaacgcat
     8281 gggccatatc gaattggctg cgcccgtcgc acatatttgg ttcttaaaat ccctgccttc
     8341 ccgcttgggt atggtgttag acatgacttt gcgcgatatc gaacgcgtat tgtactttga
     8401 agcatttgtg gtaaccgatc ccggtatgac tccgctgcaa cgccgccaat tgctgactga
     8461 agacgattac tacaacaagc tggacgaata cggcgacgac ttcgatgcca aaatgggtgc
     8521 agaaggtatc cgcgaattgc tgcgtacctt gaatgtagca ggcgaaatcg aaatcctgcg
     8581 ccaagagttg gaatcgaccg gttccgacac caaaatcaaa aaaatcgcca aacgcttgaa
     8641 agtattggaa gctttccatc gttccggtat gaagctggaa tggatgatta tggatgtgct
     8701 gccggtattg ccgcctgatt tgcgtccgtt ggttccattg gatggcggtc gttttgccac
     8761 ttccgatttg aacgatttgt accgtcgcgt catcaaccgt aacaaccgtc tgaaacgtct
     8821 gttggaactg catgcgcccg acattatcgt ccgcaacgaa aaacgtatgt tgcaagaagc
     8881 agttgactcg ctgttggata acggccgtcg cggtaaagcc atgaccggcg ccaacaaacg
     8941 cccgctgaaa tcattggcag acatgattaa aggtaaaggc ggtcgcttcc gtcaaaacct
     9001 gttgggcaaa cgtgtggact actccggccg ttccgtgatt accgtaggcc catacctgcg
     9061 tctgcaccaa tgcggtctgc cgaaaaaaat ggctttggaa ttgttcaaac cgttcatttt
     9121 ccacaaattg gaaaaacaag gtttggcttc tactgttaaa gctgcgaaaa aattggtaga
     9181 acaagaagta cctgaagtat gggatatctt ggaagaagtc atccgcgaac atccgattat
     9241 gttgaaccgt gcgccaaccc tgcaccgttt gggtattcaa gcgttcgaac ccatcctgat
     9301 tgaaggtaaa gccattcagt tgcatccgtt ggtatgtgcc gcatttaacg ccgactttga
     9361 cggcgaccaa atggcggtac acgttccatt gagcttggaa gcacaaatgg aagcacgcac
     9421 gctgatgctg gcttcaaaca acgtattgtc tcctgccaac ggcgaaccga ttatcgtacc
     9481 ttcccaagac atcgtattgg gtctgtacta catgacccgc gaccgtatca atgccaaagg
     9541 cgagggcagc ctgtttgccg atgtgaaaga agtgcatcgc gcataccata ccaaacaggt
     9601 cgagctgggt acgaaaatta ccgtacgtct gcgcgaatgg gtgaaaaacg aagcaggtga
     9661 gtttgagcct gtcgttaacc gttacgaaac aaccgtcggc cgtgcattgt tgagcgaaat
     9721 cctgccgaaa ggcctgccgt ttgaatatgt caacaaagcg ttgaagaaaa aagaaatttc
     9781 taaactgatt aacgcatcgt tccgcctgtg cggcttgcgc gatacggtta tctttgctga
     9841 ccacctgatg tacaccggtt tcggatttgc ggcaaaaggc ggtatttcca ttgccgttga
     9901 cgatatggaa attccaaaag aaaaagcggc cttgctggct gaagccaatg ccgaggttaa
     9961 agaaatcgaa gaccaatacc gtcaaggttt ggttaccaac ggcgaacgct acaacaaggt
    10021 ggtcgatatt tggggtcgtg ccggcgataa aatcgctaaa gcgatgatgg acaacctgtc
    10081 caaacagaaa gttatcgacc gtgacggcaa cgaagtcaat caagagtcat tcaactccat
    10141 ctatatgatg gcggactccg gtgcccgtgg ttctgcggct cagattaaac agttgtccgg
    10201 tatgcgcggg ttgatggcta aacctgacgg ctcgattatt gaaacgccga ttacctcaaa
    10261 cttccgcgaa ggtttgaccg tattgcaata ctttattgcg actcacggtg cgcgtaaagg
    10321 tttggcggat accgcgttaa aaaccgcgaa ctccggttac ctgacccgtc gtctggtaga
    10381 cgtaactcaa gatttggtcg ttgttgaaga cgattgcggt acttcagacg gctttgtcat
    10441 gaaggcagtg gtacaaggcg gtgatgtgat tgaagcattg cgcgatcgta ttttgggccg
    10501 tgttactgcg tctgacgttg tcgatccgtc aagcggcgag accttggttg aagccggtac
    10561 gttgttgact gaaaaactgg tggatatgat tgaccaatcc ggtgtcgatg aagtcaaagt
    10621 ccgtactccg attacttgta aaacccgcca cggtttgtgc gcgcactgtt atggtcgcga
    10681 cttggcgcgc ggtaagctgg ttaacgccgg tgaagcagtt ggtgtgattg ctgcacaatc
    10741 cattggtgaa ccgggtaccc agttgaccat gcgtacgttc cacattggtg gtgcggcatc
    10801 ccgtgcggca gcagccagcc aagtggaagc caaatccaac ggtacggcac gtttcagcag
    10861 ccagatgcgt tacgttgcca acaacaaagg tgagttggtt gtcatcggtc gctcttgcga
    10921 agtcgtgatt catgatgaca tcggccgtga acgcgaacgc cacaaagtac cttacggtgc
    10981 catcctgctg gtacaagacg gagcggccat taaagccggt caaaccttgg caacctggga
    11041 tccgcatacc cgtccgatga tcaccgaaca tgcaggtatg gtgaaatttg agaacgtgga
    11101 agagggtgtt accgttgcga aacaaactga cgatgtaacc ggtttgtcca ctctggtagt
    11161 gattgacggt aaacgtcgtt cctctagtgc ttctaaactg ctgcgtccga ctgtaaaatt
    11221 gttggatgaa aacggcgtgg aaatctgtat tcccggtacg tccactccgg tttcgatggc
    11281 attccccgtt ggtgcggtga ttaccgtacg cgaaggtcag gaaatcggta aaggcgacgt
    11341 attggcgcgt atcccgcaag cctcttccaa aacccgcgac attaccggtg gtctgccgcg
    11401 cgttgccgaa ttgtttgaag cacgcgtgcc gaaagatgcc ggtatgttgg cggaaattac
    11461 cggtaccgtt tccttcggca aagagaccaa aggcaagcaa cgtctgatta ttaccgacgt
    11521 ggacggcgta gcatacgaga ctttgatttc caaagagaaa cagattttgg tacacgacgg
    11581 tcaagtggta aaccgcggcg aaaccattgt ggacggcgcg gtcgatccgc acgatattct
    11641 gcgtctgcaa ggtatcgaag cactggcacg ctacattgtc caagaggtgc aagaggttta
    11701 ccgtctgcaa ggtgtgaaga tttctgataa acacatcgaa gtcatcatcc gtcaaatgtt
    11761 gcgccgtgtg aacattgcgg atgccggcga aaccggcttc attaccggag agcaggtcga
    11821 acgcggcgat gtgatggcgg ccaatgaaaa agctttggaa gaaggcaaag aaccggcgcg
    11881 ttacgaaaac gtattgctgg gtattaccaa agcttccctg tctaccgaca gcttcatttc
    11941 tgctgcatcg ttccaagaaa cgacccgtgt tctgaccgaa gccgcgatta tgggcaaaca
    12001 agacgaactg cgcggtttaa aagagaacgt catcgtcggc cgcctgattc ctgccggtac
    12061 cggtttgact tatcaccgca gccgtcatca gcaatggcaa ggggtagagc aggagactgc
    12121 tgaaacccaa gtaacggatg aataatcttc ggcgcgttta ttcaatacaa aaccgcaagc
    12181 tttaagcttg cggtttttct ttgtccgatt aaggcaaaaa caagcgtttt cgtcattttg
    12241 aggcgtgtgg attattcctt aggtattttc gggccggaga ccaacgaggt ggcgggtgtc
    12301 gtcggtacgt ccggagacca aaataacttt gccagggatg ttggtttcgg cggtcaaaaa
    12361 aagtagcgtc ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt
    12421 cctttaaaaa ataaatatga aacccagaaa tctctttttt gcaggctgcc tgctgacttc
    12481 ggcgacgttt gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat
    12541 tgcgatgccg tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc
    12601 ggttcgcgat gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg
    12661 taaccggatg ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg
    12721 tcgtggaaag gatttacacc ccaaataaat ttccgttaca acaagatcaa cagcaatatg
    12781 cccgcctttt attcgcgcag cggcaaggaa cggtttgtca gtatagaaaa aacgtattga
    12841 cagtattttc ttcagtcgtc cgactgattg tgagggatgt cggtaaatat ttatcggcaa
    12901 acaagaaaat catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat
    12961 aattccactc ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat
    13021 gccaactatc aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt
    13081 gcccgcactg gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac
    13141 ccctaaaaaa cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt
    13201 tgaagtcatt tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt
    13261 gattcgcggc ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc
    13321 tttggatact gcaggtgtta aagaccgtaa acaagcccgt tccaaatatg gtgctaagcg
    13381 tcctaaataa ttactgggac ttaaataggc acgtcggccg cctaagcaga acaacggccg
    13441 agtaagtgaa tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga
    13501 aattaaaatg ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt
    13561 cggcagcgtc gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt
    13621 tgccgagcgt atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc
    13681 aatcgaagta tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg
    13741 ccgtgtaggt ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc
    13801 tttggcaatg cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct
    13861 gcgtttggca ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg
    13921 tgaagaagta caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt
    13981 ttgaaaggct aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat
    14041 ttccgcccat atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctataccgg
    14101 tttgacccac aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca
    14161 agagcaagag cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat
    14221 ggcgaaacaa ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt
    14281 taccgtagag gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc
    14341 ggtgggcggt gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt
    14401 gccgcgcttg gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt
    14461 cgagcaaatg aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc
    14521 ggaagacaac ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga
    14581 agccgataaa ggtacaacct ttacctatgg cgatattcct gccgaattgg tcgaaactgc
    14641 cgaagaatgg cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga
    14701 caaatactta ggcggcgacg agctgaccga agaagaaatc gtaggcgcgt tgcgtcaacg
    14761 tactttggca ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt
    14821 tcaacgtatg ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt
    14881 tcaaggtgtc aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa
    14941 attctctgca ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat
    15001 ccgcgtttac tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac
    15061 tcgcgaacgt atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga
    15121 agtacgcgcc ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac
    15181 cttgtgtgcg gaaagcgcgc cgattatctt ggaacgtatg gaattccccg agccggtaat
    15241 ccatattgcc gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa
    15301 ccgcttggct aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac
    15361 cattatttcc ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga
    15421 attcggtgtg gaagcaaata tcggtgcgcc tcaagtggct taccgtgaaa ctatccgcaa
    15481 agccgttaaa gccgaataca aacatgcaaa acaatccggt ggtaaaggtc aatacggtca
    15541 cgttgtgatt gaaatggaac ctatggaacc gggtggtgaa ggttacgagt ttatcgatga
    15601 aattaaaggt ggtgtgattc ctcgcgaatt tattccgtct gtcgataaag gtatccgcga
    15661 tacgttgcct aacggtatcg ttgccggcta tcctgtagtt gacgtacgta tccgtctggt
    15721 attcggttct taccatgatg tcgactcttc ccaattggca tttgaattgg ctgcttctca
    15781 agcgtttaaa gaaggtatgc gtcaagcatc tcccgccctg cttgagccga ttatggcagt
    15841 tgaagtggaa accccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg
    15901 cggtgttgta ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt
    15961 acctctggca gaaatgttcg gttactcgac cgacctgcgt tctgcaaccc aaggccgcgc
    16021 tacttactct atggagttca agaaatattc tgaagctcct gctcacatag ctgctgctgt
    16081 aactgaagcc cgtaaaggct aatcaggcaa ataggccgtc cgaaaggctg aaatgatttt
    16141 tcagacggca ttgttcttta atcgatcttt aatgtaaagg aattagc
//
LOCUS       DAWHXS010000044        15806 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWHXS010000044 DAWHXS010000000
VERSION     DAWHXS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15806
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.044"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            369..5033
                     /gene="iga"
                     /locus_tag="WIE22_001078"
     CDS             369..5033
                     /gene="iga"
                     /locus_tag="WIE22_001078"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ4127175.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLANNERDANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHASTITLTGKSLITAPQNLSVYEIRNDYDDDDYYGYYSYRKP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRVSGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKALNSFDATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQ
                     QAEAEKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQKAEAEEAKRRAA
                     EIAEQKAAAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKAEAEREA
                     AELAKQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDASDYEEI
                     PLDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAVAQ
                     ADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDY
                     ASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYG
                     KYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAG
                     IRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYG
                     KGGVNVGGNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQI
                     RF"
     gene            complement(5212..6414)
                     /gene="trpB"
                     /locus_tag="WIE22_001079"
     CDS             complement(5212..6414)
                     /gene="trpB"
                     /locus_tag="WIE22_001079"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ4127176.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASAAVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(6497..7186)
                     /locus_tag="WIE22_001080"
     CDS             complement(6497..7186)
                     /locus_tag="WIE22_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ4127177.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAVYRAF
                     FEMAGLCEKQL"
     gene            7383..8162
                     /gene="rsmA"
                     /locus_tag="WIE22_001081"
     CDS             7383..8162
                     /gene="rsmA"
                     /locus_tag="WIE22_001081"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ4127178.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8335..9063
                     /locus_tag="WIE22_001082"
     CDS             8335..9063
                     /locus_tag="WIE22_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ4127179.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9136..9495
                     /locus_tag="WIE22_001083"
     CDS             9136..9495
                     /locus_tag="WIE22_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127180.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            9500..9946
                     /locus_tag="WIE22_001084"
     CDS             9500..9946
                     /locus_tag="WIE22_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ4127181.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAATLAGSSMVTPYALF
                     LQWDTGGKTVRHCIMPDMTDKESYRKLKVWVLWRQPKKTAETDTSD"
     gene            9977..11251
                     /gene="folC"
                     /locus_tag="WIE22_001085"
     CDS             9977..11251
                     /gene="folC"
                     /locus_tag="WIE22_001085"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ4127182.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGMFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLETVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            11264..12262
                     /gene="ftsN"
                     /locus_tag="WIE22_001086"
     CDS             11264..12262
                     /gene="ftsN"
                     /locus_tag="WIE22_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ4127183.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESRKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAVGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            12255..12752
                     /locus_tag="WIE22_001087"
     CDS             12255..12752
                     /locus_tag="WIE22_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ4127184.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            12960..14504
                     /gene="purF"
                     /locus_tag="WIE22_001088"
     CDS             12960..14504
                     /gene="purF"
                     /locus_tag="WIE22_001088"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ4127185.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            14604..15095
                     /gene="greB"
                     /locus_tag="WIE22_001089"
     CDS             14604..15095
                     /gene="greB"
                     /locus_tag="WIE22_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ4127186.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            15154..15780
                     /locus_tag="WIE22_001090"
     CDS             15154..15780
                     /locus_tag="WIE22_001090"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ4127187.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         4410 a         4295 c         3857 g         3244 t
ORIGIN      
        1 gaacccccat ctcgtcatta ccacgaaagc gggaatctag gacgcagggt taagaaaacc
       61 tacatcccgt cattccctca aaaacagaaa accaaaatca gaaacctaaa atcccgtcat
      121 tcccgcacaa gcgggaatcc aaaaacaaaa agccgcagaa atttaacaaa agaaccgaac
      181 cccgaaaacc ggcttcccgc cttcgcttga ataacagaac aaaggaagcg gaaataaaaa
      241 catcgggatt ctttttttaa attgacaata aaacaccaaa attaatgatt tacacaacaa
      301 aaaatagtca acaccgccaa accggcgtaa aatacaagac attatcaccc ttaaaacggt
      361 aaaaccttat gaaaaccaaa cgttttaaaa ttaacgccat atccttatcc atctttcttg
      421 cctatgccct tacgccatac tcagaagcgg cattggtcag agacgatgtc gattatcaaa
      481 tattccgtga ctttgcagaa aacaaaggca aattttttgt cggcgcaacc gatttatcag
      541 tgaaaaacaa acaaggtcaa aacatcggca acgcattatc caacgtaccg atgattgatt
      601 ttagcgttgc agatgtcaac agacgtacat taaccgtcat cgatccccag tatgccgtca
      661 gcgtcaaaca tgtaaaagga gacgaaatct cttattacgg gcatcacaat gggcacttag
      721 atgttagcaa tgatgaaaac gaatatcgtt cagttgcaca aaatgactac gaaccaaata
      781 aaaattggca tcacggtaat caaggtcgtc ttgaagacta caacatggca cgccttaata
      841 aattcgtaac agaagtcgca cctattgcac caaccagtgc aggcggcggc gttgaaacct
      901 ataaagataa aaaccgtttt tctgagtttg tgcgagttgg agcaggtacg caatttgaat
      961 ataacagccg ctacaatatg acagagcttt cacgagctta tcgttatgcc attgcaggta
     1021 cgccttatca agacgtgaat gtcacatcga atctaaatca agaaggcttg attggcttcg
     1081 gtgataattc aaaacatcat tccccagaaa aactcaaaga agttttatct caaaacgcat
     1141 tgactaacta tgctgtgtta ggcgatagcg gttcaccatt atttgcttat gataaacaag
     1201 aaaaacgctg ggtcttttta ggtgcttacg attattgggc aggctatcaa aaaaactctt
     1261 ggcaagaatg gaatatctat aaaaaagaat ttgcagatga aatcaaacaa cgcgataacg
     1321 ccggcaccat caaaggttat ggcgaacatc attggaaaac cacgggcaca aacagccata
     1381 tcggttcgac agcggtaagg cttgccaaca atgaaaggga tgcaaacaac ggacagaatg
     1441 ttacctttga aaacaacggc actttggtat tggatcaaaa catcaaccaa ggcgcgggcg
     1501 gtctgttttt caaaggcgat tacacagtca aaggtataaa taatgacatc acttggctag
     1561 gtgcggggat tgatgttacc gacggcaaaa aagtcgtttg gcaagtcaaa aatccgaatg
     1621 gcgacagatt ggcaaaaatc ggcaaaggca cattagaaat aaacggcaca ggcgttaacc
     1681 aagggcaatt aaaagtcggc gacggtacgg ttattctgaa tcaacaagcc gatgccgaca
     1741 aaaaagtcca ggctttctcc caagtcggca ttgtgagcgg acgcggtaca ttggtattaa
     1801 atagttcaaa tcagattaat cccgataact tatatttcgg tttccgtggc ggtcgtttgg
     1861 atgccaatgg caatgacttg acttttgaac acatccgcaa cgtggatgaa ggcgcgcgca
     1921 ttgtcaacca caacacaggt cacgcctcca caatcacatt aaccggcaaa tcattaatta
     1981 ccgctcctca aaatctttct gtttatgaaa tccgaaatga ttacgatgat gacgattatt
     2041 atggctatta cagctataga aaacccattc cgcaaggcaa agatctttat tacaaaaact
     2101 atcgttatta cgccctaaaa tccggcggca gcgtgaacgc accgatgccc gaaaacggac
     2161 aaacggaaaa taacgactgg attttaatgg gcagcacaca agaagaagcc aagaaaaacg
     2221 cgatgaacca caaaaacaat cagcgtgtta gcggttttag cggtttcttt ggcgaagaaa
     2281 acggaaaagg acataacggt gcattaaacc ttaatttcaa cggcaaaagc gcgcaaaacc
     2341 gtttcttgtt aacaggcggc actaatttaa acggaaaaat aagcgtaact caaggcaatg
     2401 tcttgttatc aggtcgtcca acaccacacg caagagattt tgtgaacaaa tcttcagccc
     2461 gaaaagacgc acatttctcc aaaaacaatg aagtcgtatt tgaagacgac tggataaacc
     2521 gcacattcaa agccacagaa attgcggtta atcaatccgc atcattctct tccggtagaa
     2581 atgtatccaa catcaccgcc aacatcaccg caacagacaa cgccaaagta aatttgggtt
     2641 acaaaaacgg cgatgaagtt tgcgtgcgct cggactatac cggttacgtt acctgcaaca
     2701 cagacaactt atccgataag gctttaaaca gctttgatgc gacacagatt aacggcaatg
     2761 tgaatttgaa tcagaatgcg gcattggtct taggcaaggc tgcattatgg gggcaaattc
     2821 aagggcaagg aaacagccgt gtcagcctaa accaacatag caaatggcat ttgaccggcg
     2881 acagccaagt acacaatctg tcattggcgg atagccatat tcatttgaac aacgcttccg
     2941 acgcgcaaag tgcaaataaa taccacacgc tcaaaatcaa tcatttatcc ggtaacgggc
     3001 attttcatta tctgacgcac ctggcgaaaa atcttgggga taaggtgctt gtgaaggaat
     3061 ccgcatccgg tcattatcag ctccatgttc aagataaaac aggcgaaccc aatcaggaag
     3121 ggctgaatct ctttgatgca tcatccgtac gagaccgctc ccgcctttct gtttccttgg
     3181 cgaataacca tgtagattta ggcgcattgc gttatacaat caaaacagaa aacggtatta
     3241 cccgattgta caatccttat gccgagaacc gccgccgagt caaaccggcc ccgtctcctg
     3301 ccacaaacac ggcttctcaa gcacaaaagg caacacaaac ggacggtgca caaattgcca
     3361 agcctcaaaa tatcgtcgtc gcaccgccta gcccgcaggc aaatcaagcc gaagaagcaa
     3421 aacgccagca agcaaaagcg gagcaagtga agcgtcagca agcagaggca gaaaaagtcg
     3481 cacatcaaaa ggctgaagaa gcgaaacgtc agcaagatgc ccttgcccgc caacaagcag
     3541 agcaagaacg ccaacgattg gaagccgaac gccaagctgc cgaaattgcc aaacaaaaag
     3601 cagaagccga agaagcgaaa cgccgagccg ctgaaattgc cgagcaaaaa gcagctgccg
     3661 aagaagcaaa acgccaagct gccgaacttg cacgccaaca agaagaagca cgaaaagctg
     3721 cagagttggc tgctaagcaa aaagcggaaa ctgaacgcaa agcagcagaa attgccgagc
     3781 aaaaagcgga agccgaacgt gaagccgccg aacttgccaa gcaaaaggcg gaagaagaag
     3841 gtcgccaagc agcacaatct caacccaaac gtcgtaaccg cagggctata ccaccggaac
     3901 tgtcatctga tgcaacaacc cgagctttgc ctaggatagc gagaaattca aatcctgatg
     3961 ccagcgatta tgaagaaatc cccttggatg cgttggaaga tgaagatgta tccgaatcgg
     4021 ttgacacatc agacaaacag cctcaagaca atacggaact tcatgaaaaa gttgagacag
     4081 tgagtttgca accaagagcc gctcagccgc gagcccaagc cgccgcgcag ccgcaagccc
     4141 aagccgtcgc gcaagccgat gcagtcagca ccaataccaa ctcggcttta tctgacgcaa
     4201 tggcaagcac gcaatctatc ttgttggata caggtgcttc attaacacgg cacattgcac
     4261 aaaaatcacg cgctgatgcc gaaaaaaaca gcgtttggat gtcgaacatc ggttatggcc
     4321 gtgattatgc ttccgcacaa tatcgccggt ttagttcgaa acgcacgcaa acacaaatcg
     4381 gcattgaccg cagcttgtcc gaaaatatgc agataggcgg cgtattgact tactctgaca
     4441 gtcagcatac ttttgatcag gcgagcggca aaaatacttt tgtgcaagcc aacctttatg
     4501 gtaagtatta tttaaatgat gcttggtatg tggccggcga tattggtgca ggcagcttga
     4561 gaagccggtt acaaacacag caaaaagcaa actttaaccg aacaagcatc caaaccggcc
     4621 ttactttggg caatacgctg aaaatcaatc aattcgagat tgtccccagt gcgggaatcc
     4681 gttacagccg cctgtcatct gcagattaca agttgggtaa cgacagtgtt aaagtaagtt
     4741 ctatgtcagt gaaaacactg acggccggac tggattttgc ttatcggttt aaagtcggca
     4801 accttaccgt aaaacccttg ttatctgcag cttactttgc caattatggc aaaggcggcg
     4861 tgaatgtggg cggtaattcc ttcgcctata aagcagataa tcaacagcaa tattcagcag
     4921 gtgccgcgtt actgtaccgt aatgttacat taaacgtaaa tggcagtatt acaaaaggaa
     4981 aacaattgga aaaacaaaaa tccggacaaa ttaaaataca gattcgtttc taaaatacta
     5041 aattcatagc aaaataaaat gccgtctgaa ctcaggcttc agacggcatt tttatagtgg
     5101 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggcaaggc
     5161 gaggcaacgc cgtactggtt tttgttaatc cactatatca gacggacgag gttacagttt
     5221 aatccctttg agcttcgcga cggtattgat gtctttgtcg ccacgaccgg ataggttgac
     5281 cagaatcact tggtctttgc ccattttcgg cgcgtttttc accgcccaag caacggcgtg
     5341 gctggattcg agcgcgggga tgatgccctc gaagcggcag agcaaatcaa aggcttcgag
     5401 tgcttcgtcg tctttagcga cggtgtattc gacgcgcttg atgtcgtgca gatggctgtg
     5461 ttccgggccg atgccggggt aatccaagcc tgcggaaaca gagtgtgtac ccaagacttg
     5521 accgttttcg tcctgcatca gatagctgcg gaaaccgtgc aatacgccaa tcggtgagcc
     5581 cgaagtaatc ggcgcggcgt gatcgggggt gttcacgccc aaaccgccag cctccacgcc
     5641 gacgaggcgc acgttttctt cgccgatata cgggtggaac aaaccaatgg cgttcgatcc
     5701 gccgcccacg caggcaacgg caacgtcggg ctgtctgccg atggcttcca acatttgttc
     5761 tttggcttca ttgccgatca cgcattgaaa gtcgcgcacc atttcgggat acggcgcggg
     5821 accggcggcg gtaccgatga tgtagaatgt gtcatccacg cgggcgaccc attcgcgcat
     5881 ggcttcgttc atcgcgtctt tcaaagtacg gctgccgctg tcaacgccga cgacattcgc
     5941 gcccaataat ttcatacgga acacgttggg catttggcgt tggatgtcgt ccgcacccat
     6001 gtacacgtcg caagtcatgc cgaagcgggc ggcaacagcg gcactcgcca cgccgtgctg
     6061 acccgcgccg gtttcggcga tgacgcgttt tttacccata cgttttgcca acagtgcctg
     6121 accgatggtg ttgttgactt tgtgcgcgcc ggtgtggttc aagtcttcgc gcttcaacca
     6181 gatttgcgca ccgcccaaat gctcggacaa ccgcgcggcg tggtaaacgg ggctgggcct
     6241 gccgacataa tgtttcaaat cgcggtggaa ttcttcccaa aacgacgggt cgtttttcgc
     6301 tgccttatag gcatccgcca gctcttgcaa agcgggaatc agggtctcgg acacgaacag
     6361 cccgccgtgt tcgccgaaaa agcccttctc gtcgggcgcg tggtagttct tcatcggatt
     6421 cttccttgtg cttcaggttt tcaaacggca tttccgtttt ttcaaacatg ccgtccgaac
     6481 gggaaattgt cggcaattat aactgctttt cacacaagcc cgccatttcg aaaaacgccc
     6541 gatacaccgc cgcctttttc gccaaactca aaggataggc gcgcgaagtc gaaggcaggc
     6601 gcgtcagcgt caaatcccgt ccggcaaacg gaaacggcac ggtttcgccc gttttcggca
     6661 ttttgatgcc gccaccctga atatcgagca ggatttccgt cgccttgccg cctgtcgtac
     6721 aaatatggcg gcactcgggt atttttgcca acaccgccgc caaatcgacg gtttcaacta
     6781 cctttaaaaa cttgtcggac gcattgccgt gttcccttgc cgccttcaac acggtcggac
     6841 aggacgcaat cccccgttcg tgcaaaaacg ccttgatttt ctcggcatca aacgcttttt
     6901 cagacaacct ttggaaatgc gccgcatcat taaaaaacac cagcccgtaa acgcgccaca
     6961 tatcgttttg gaaattcgga taatgaaact gcatcgcgcg tttgtcttcc ttgggcggaa
     7021 acgtccccat catcatgacc gcagccttcg gcggcaacac ggaatcgaag ggatgggttt
     7081 cgatttcaag cagattttca gacacgttca atcctccaac aggcaaaacg gcagacagcc
     7141 ggtttttgca ccaaaccgcc aaacacggca agccttcaaa cagcattatc acgccctgcc
     7201 gttttcggtt acaatgccga catcaaacgg atactgtaaa cacatttatg ttccaacaca
     7261 caggacgaca cttaaagcac cgccctatgt gccatcctga tttggaagga gttacgcccc
     7321 tcccaaatag agtctgattc taccgcccta aagggcgggg tttcaaccga aaaggaaaca
     7381 cgatgaaaga acacaaagcc cgcaagcgtt tcgggcagaa ttttttgcag gacacgcgga
     7441 tcatcagcga tattgtcaac gccgtgcgcc cgcaggcgga tgatgtcgtg attgaaatcg
     7501 gcccgggttt ggcggcgatt accgaacctt tggcgaaaaa gctgaaccgc ctgcacgttg
     7561 tcgaaatcga ccgcgacatc gtatgccgtc tgaaaacgct gccgtttgcg gataaactgg
     7621 tgattcacga aggcgatgta ttgcagtttg atttcaacgg catcgcaggc aaaaagaaaa
     7681 tcgtcggcaa cctgccctac aacatttcca cgccgctttt gttcaagctg gcggaggtgg
     7741 cggacgatgt cgtcgatatg cactttatgc tgcaaaaaga agtggtcgat cgtatggttg
     7801 ccgcgccgaa aagcaacgac tacggccgct tgggcgtgat gctgcaatat ttcttcgata
     7861 tggaaatgct gattgacgtg ccgcccgaat cgttcgaccc cgcgccgaaa gtggattcgg
     7921 ccgtcgtccg catgattccg gtgaaacacc gtatcggcaa ggcggacgat ttcgagcatt
     7981 tcgccaaact cgtgaaactc gccttccacc aacgccgcaa aaccatacgc aacaatctga
     8041 aagagcttgc aggcgacgat gatttgcagg cagtcggcat caatccgcaa gaccgcgccg
     8101 aacatatcgc gccggagaaa tatgtggcgt tgagcaatta tctggcgggc aaggccgtct
     8161 gaaaagggag taagccgatg acgaaatacc tgcttttacc cctgatactg ccgcttgcag
     8221 cctgcctgac caatccgaac gaccgttttt ttgacggcag acgctacacg cttcatcata
     8281 tggagctccc gtaacgacaa cctgcaacgg caaatgccgt ctgaaagaaa agacatgatc
     8341 aaattcaaaa acgtacacaa acatttcaaa gacctgcacg tcatcaacgg cgtgaacttg
     8401 gaaatcaaaa aaggcgaagt ggtcgtcgtc tgcggacctt cgggcagcgg caaatccaca
     8461 ctcatccgca ccgtcaacca gcttgaaagc atcgaaagcg gcgagatttg ggtggacggt
     8521 gtcaacgttg ccgacccaaa aaccgatttg aacaaaatcc gcgaagaagt cggctttgtg
     8581 tttcaaagtt tcaacctcta cccccacctg accgtattgg aaaacatcac cctcgcaccg
     8641 atgaaggtta aaggtcaaaa tgcggaacag gcggagaaaa aagcgatgga acttttggaa
     8701 cgcgtcggac tggcgcacaa aaaagacgct tttccgtccc aactttccgg cggccagcag
     8761 cagcgcgtgg cgattgcacg cggtttggcg atggaaccgc gcgtaatgtt gtttgacgaa
     8821 ccgacctccg cactcgaccc cgaaatggtc ggcgaagtgt tgaaagtgat gaaagacttg
     8881 gcagaaagcg gcatgaccat gatgtgcgta acccacgaaa tggggtttgc ccgcgaagtt
     8941 gccgaccgcg tgattttcgt cgataaaggg caaatcctcg aagacgaaac gcccgaagcg
     9001 tttttcacca acccgaaaca cgaacgcgcc aagcagttcc tgcaacaggt catgacccac
     9061 tgatgctata ccgtctgaaa accgaacgtt cagacggcat agcccgacac cgcaagatga
     9121 ttgagaaaga aaaccatgta ttttgttgac cgcaccgccg tcgtcctcaa gccgaccgtc
     9181 cgctttttgg aatggctcaa aagcaccgac gaaaatatgc ccgacctgac catagagcag
     9241 cttcgtgcca actgctccgt cttcctcgtt ccgcagttcg acgaaccgga agccgtcgtt
     9301 tcctatttcg acgaacgcta ccgccagatt tttgaagccg aactcgccgg ctgggacatc
     9361 gacaaggata aatggccgca agatatgggg ctgaaggcgt tttgggaatt tttcgacatc
     9421 gaaatccacg atatggtgtt ggatatggaa gaagcggatt tgaacatcac gcccgtgttc
     9481 gacaatatga tgtaacgccg tgcgcgcctt tcagacagca ttgaggccgt cccgcatcct
     9541 gaacatcctg accgtatcgc tccactcggc ttcccttgcc gtctgcctga cctggtttta
     9601 cggccggatg atgtggttcg gtttggcggc tttggcggca agttacgcat actcgctcag
     9661 gataacgaat ctgaaacacc gccatgccat aaccgccatt accatagacc gcgacggaca
     9721 ggcggaaatc gtatccggca aagaaaaaac agcaaaggcg gcaacacttg cgggcagcag
     9781 tatggttacg ccttatgcct tattcttaca atgggacacc ggcggcaaaa ccgtccgcca
     9841 ctgcatcatg cccgacatga cggacaaaga atcctaccgc aagctgaaag tctgggtact
     9901 ttggcggcag ccgaaaaaaa ccgccgaaac cgatacatca gactgatttc agacggcatt
     9961 tgaaaacgaa acactcatga aaacactaca agactggctc tcgcatttgg aaaccgccca
    10021 cagcggcggt ttgatcgaca tgggtttgga gcgcacgagc gaagtgaaaa aacgcatgaa
    10081 gctcgagccg caatgccccg tcgtcgttgt cgcgggaacc aacggcaaag gttcggtctg
    10141 tgcctacctg acacagattt acaaacaggc gggctacaaa accggtacgc tgaccagccc
    10201 gcatttattg cgttacaacg aacgcatcgc catcaacgcc gaacccgttt cggacgatac
    10261 gattattgcc tctttcgagc gcatcgaagc ggcgcgcagc gaaatatcgc tgacttattt
    10321 tgaattcaat acgttggcgg ccgtcgacat cttcatgcgc gaacaggttg acgtgatgat
    10381 attggaagtc ggcttgggcg gacgcttgga cgcggtcaac gcatttgacg gcgattgcgc
    10441 ggtggttact agtgtagatt tggaccatca ggcgtttttg ggcgatacgg tcgagcaggt
    10501 cggctttgaa aaagcaggca tgttccgcag cggcaaaccc gcaatctgcg gtcaaaaccc
    10561 cgcgcccgaa tcgttgcttt cacacgccga agccataggc gcgaaactgc tgatggtgca
    10621 gcgcgatttc gagtttcacg cgatggaaaa catccaatgg aactaccgct tccggccgca
    10681 acattcagac gaccccgcgc gcaaccgcaa tgccctgccc ttccccgcat tgcgcggcac
    10741 ataccaactt tccaacgccg cctgcacgtt gaccgtattg gaatgcttga acgccaaatt
    10801 gccggtggac atcggcgcca tcaaacgcgg attgctgctg gttgaaaatc ccggacgctt
    10861 ccaagtcctg cccggccgtc cgctgaccgt tttggatgtc ggacacaatc cccacgctgc
    10921 ccgcgccctg cgccgcagcc tgattaattt ggcgtttgcg caaaaacgca ccgccgtgtt
    10981 cagcatgttg tccgacaaag acatagacgg cgtgttggaa accgttaaag accagtttga
    11041 cgagtggtat atcgcaccgt tggatgtacc gcgcggcatg acgctcgaga gcctgcaaaa
    11101 caaactgcac gaacacagca tcgaaaacat acaaacattc gccgccgtcc gcgaagcata
    11161 ccgggcggca gcctcaaaag cgggcgagaa cgacagaatc gtcgttttcg gctcatttca
    11221 taccgttgcc gatgtcatgt cggtacttta aaaggaaact tctatgtccg aaaacaaaca
    11281 aaacgaagtc ctgagcggtt acgaacaact caaacggcgc aaccgccgcc gcctcgtaac
    11341 ggcaagttgc ctggttgccg cctcctgcat cctgctggca gccgccctca gttccggccc
    11401 tgccgaacag actgccggcg aaacaagcgg cgtagaaaac aaagcggcag gtgcggcaca
    11461 aacccctgcc ttgaaatccg ccgccgacaa accgcaggac ttggcaggcg aagacaagcc
    11521 ttctgccgcc gacagcgaaa tcagcgagcc tgaaaacgta ggcgcgccgc tggtgctgat
    11581 taacgaccgc ctcgaagaca gcaacatcaa aggtttggaa gcatccgaga aactgcaaca
    11641 ggcagaaacc gccaaaaccg caccgaagca ggcaaaacaa cgcgctgccg aaaaagtgcc
    11701 ggcaactgcc gacagtacgg atacggtagc ggttgaaaaa ccgaaacgca ctgccgaaac
    11761 aaaaccgcaa aaagcggaac gcactgccaa agccaagccc aaagccaaag aaaccaaaac
    11821 cgccgaaaaa gttgccgaca aaccgaaaac tgccgccgaa aaaaccaaac cggatacggc
    11881 aaaatccgac agcgcggtaa aagaagcgaa aaaagccgac aaggctgaaa gcagaaaaac
    11941 agccgaaaaa gaccgttcgg acggcaaaaa acacgaaacg gcacaaaaaa ccgacaaagc
    12001 ggacaagacc aaaaccgccg agaaggaaaa atccggtaaa aaagccgcca ttcaggcagg
    12061 ttatgccgaa aaagaacgcg ccttaagcct ccagcgcaaa atgaaggcgg tgggtatcga
    12121 ttcgaccatc accgaaatta tgaccgacaa cggcaaagtt taccgcgtca aatcaagcaa
    12181 ctataaaaac gcaagggatg ccgaacgcga tttgaacaaa ttgcgtgtac acggtatcgc
    12241 cggtcaggta acgaatgaat aacctccccg ttgccgacct cctcgtcttc gccatcatcg
    12301 ccgcctgcat cgtgctatcc gcgatgcgcg gcgtgattgc ggaggcaggt tcgatggcgg
    12361 catgggtggt ttccttcttt tttgccaaac tctttgccgc acccttcgcc gacctcgcct
    12421 ttgcctcgtt ccaaccccgc ctgtttgcat tggctctgtc gttcatttcc ctgttcgtca
    12481 ttgcctgtct gatccagaaa atgctccgtt cgctgctgac cggcgcagtt tcggcggtcg
    12541 gtttgggctt tgccaaccgc attttgggcg gcgtattcgg tgcattgaaa ggcgttttga
    12601 ttgttaccct gctggtcatg cttgcttcaa aaaccgacct gcccgatacc gaagagtggc
    12661 agcagtccta taccataccg tttttcgtat cgctttccga agccgtgttg aaccatagcg
    12721 gcggcacggc ggaaactccg gaagacgatt gattgccata cggatatttc agacggcatt
    12781 ctcttttatg ccgtctgaaa tgttttttcc ttttctcagc catatcacag attaaatctt
    12841 atcctccata aacccaccta tctgtccttc gctcaaatat tgtcaacaat tcaggcaaaa
    12901 aaccaccgtt tgctttataa ttccgtttta cccttaaacc tcgaatcgga gaagacgata
    12961 tgtgcggcgt attaggtttg gtcagtcatg aacccgtgaa ccagcttctg tacgacggtt
    13021 tgcagatgtt gcagcacagg gggcaggatg cggcgggcat tgcaacggcg gaaggcggca
    13081 ccttccatat gcacaaaggc aaagggatgg tgcgcgaagt gttccgcacg cgcaatatgc
    13141 gcgatttggt cggcaacgcc ggcatcgccc acgtccgtta tcctacggcg ggcaacgccg
    13201 gcagcagcgc ggaggcgcag cctttctacg tcagctcgcc tttcggcatc gttttggcgc
    13261 ataacggcaa tctcaccaac acggccgaac tgtatgaaaa cgtgtgcaac aaacacctgc
    13321 gccacgtcaa caccagctcc gattccgaag tcttgctcaa cgtattcgcg cacgaattac
    13381 gccgcgaagt ctccaaaaac gccgatccgc accggctcaa tgccgacaat attttcaacg
    13441 ccgttgccga agtccaccgc ctagtgcgcg gcgcatacgg cgtggttgcc atgattgcgg
    13501 gctacggtat gcttgccttc cgcgaccctt acggcatccg cccgctggta ttgggttcgc
    13561 aaaccgacaa cgaaggcagg aaatcctatg ccgtcgcctc cgaatccgtc gccttcaacg
    13621 cgcttgccta cgatttggaa cgcgacatcc ggccgggcga agcggtattt gtcggctttg
    13681 acggcacaat gattgcccgc caatgcagcg accgtgccaa actcagcccc tgcctttttg
    13741 aatatgttta ttttgcccgc cccgactccg taatcgacgg cgtatcggtt taccaatcgc
    13801 gcttggatat gggcgtgtcc ttggcggaaa aaatcaaacg cgagctgccc gtggacgaca
    13861 tcgatgtcgt gatgcccatt cccgacacca gccgccccag cgcgatggag cttgccgtcc
    13921 acctcgacaa accctaccgc gagggcttga ttaaaaaccg ctatatcggc cgcaccttta
    13981 ttatgcccgg ccaggcgacg cgcaaaaaat ccgtgcgcca aaaactcagc ccgatggaaa
    14041 ccgagtttgc aggcaaaagc gtgttgctgg tggacgactc catcgtgcgc gggacgacca
    14101 gccgcgaaat cgtcgaaatg gtacgcgcgg cgggcgcgcg caaagtctat atcgcctccg
    14161 ccgcccccga agtgcgctat cccaacgtat acggcatcga tatgcccacg cgcgaagagt
    14221 tgattgccaa cgggcgcagc gcggcggaaa tcgccgccga aatcggcgcg gacggcatcg
    14281 tgttccaaaa tttgagcgat ttggaagccg tcgtcaaagc actcaatccg aaaatcgaat
    14341 ccttcgattc gtcctgtttc aacggcattt atcagaccgg cgacatcgat gatgcctacc
    14401 tcgaccgcct gtctgccgag aaatccggct gcggcggcct gaaaatccac ccgagtagga
    14461 tggaacacag catcagcatc agcgatgcgg gcgacgaaga ataaggcttc ccaaaccgcg
    14521 atgccgtctg aaatccgaac gccgggtttt cagacggcat ttcccatatc ggcatcacat
    14581 ttcccatttc atccgcacgc aatatgagta ccgaaaccaa aaactacatt acgcccgccg
    14641 gatggcaggc attgaaagac gagctttatc aactggttaa taaagaacgc cccgaaatcg
    14701 tccaaatcgt caactgggcg gcaggcaacg gcgaccgcag cgaaaacggc gactatcttt
    14761 acggcaaacg ccgtatgcgc gaaatcgacc gccgcatccg tttcctgacc aagcgtttgg
    14821 agtccgccgt cgtcgtcgat cccgaattgc gcgaagcgac cgaccaagta tttttcggcg
    14881 cgacggtcgg gctgttgcgc ggcgacgggc gcgagcaaac cgtcaaaatt gtcggcatcg
    14941 atgaaatcga taccgcgcaa aacaaaattt cttggatttc gccgttggcg cgttgcctga
    15001 ttaaagcgcg cgaaggcgac gaagtggttt tgaacacgcc ggaagggcgt gaggaaattg
    15061 aaatcctttc tgtcgaatac atcaaaatcg actgatcggg aatgccgtct gaaacggcac
    15121 aaacgcatcg tttcccaaat aaaggaatac cgaatgagga aaatccgcac caaaatctgc
    15181 ggcatcacca cactggaaga cgcagcggcc gccgcagcgg caggtgcgga tgccgtcggg
    15241 ctggtctttt tccaaggcag cagccgggcc gtcgatattg cccgcgccaa aaaaatcacc
    15301 gccgcactgc cgccgtttgt cagcgttgtc gcccttttcg tcaacgaaag cgcgcaaaac
    15361 atccgccgca tccttgccga agtgccgata cacatcatcc aattccacgg cgacgaagac
    15421 gacgcattct gccgccagtt cgaccgcccc tatatcaagg ccattcgtgt tcagacggca
    15481 tcagacatcc gaaacgccgc cgaccgcttc cccgacgctc aggcactgct gttcgatgcc
    15541 taccaccctt cggaatacgg cggcaccgga caccgcttcg actggacgct gctggcggaa
    15601 tattcgggca aaccgtgggt gcttgccggc gggctgaccc ctgaaaacgt cggcgaagcc
    15661 gtccgcatca ccggagcgga agcggtcgat gtatccggcg gcgtggaagc gtctaaaggc
    15721 aaaaaagacc cagccaaagt tgccgccttt atcgcaaccg ccaaccgcct atcccgttaa
    15781 agcaacaaaa attgccgccg gaatga
//
LOCUS       DAWHXS010000045        15350 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWHXS010000045 DAWHXS010000000
VERSION     DAWHXS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.045"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..591)
                     /locus_tag="WIE22_001091"
     CDS             complement(<1..591)
                     /locus_tag="WIE22_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ4127188.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQ"
     gene            complement(736..1167)
                     /locus_tag="WIE22_001092"
     CDS             complement(736..1167)
                     /locus_tag="WIE22_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127189.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(1183..7128)
                     /locus_tag="WIE22_001093"
     CDS             complement(1183..7128)
                     /locus_tag="WIE22_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ4127190.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKNINWNQVQLAYDRWDYKQEGLTEAGAAIIALAVTVVTSGAG
                     TGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAA
                     TAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAA
                     LVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEA
                     LTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVNAAANAAEVAVKNNQLSDKEG
                     REFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAASIAICTDISRSTECRTIRKQH
                     LIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQSYHLNTAAKSWLQSGNTKPL
                     SEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKYPEGISFDTNLKRHLANADGF
                     SQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGITRIKYEIPTLDRTGKPDGGFK
                     EISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIAQNERTKSISERKNVIQFSET
                     FDGIKFRSYFDVNTGRITNIHPE"
     gene            complement(7221..8963)
                     /gene="hrpB"
                     /locus_tag="WIE22_001094"
     CDS             complement(7221..8963)
                     /gene="hrpB"
                     /locus_tag="WIE22_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ4127191.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     SYETAIHAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRH
                     QFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            complement(9024..9272)
                     /locus_tag="WIE22_001095"
     CDS             complement(9024..9272)
                     /locus_tag="WIE22_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ4127192.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGDFGEFCKGLIL"
     gene            9868..9975
                     /locus_tag="WIE22_001096"
                     /pseudo
     CDS             9868..9975
                     /locus_tag="WIE22_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10172..10666)
                     /locus_tag="WIE22_001097"
     CDS             complement(10172..10666)
                     /locus_tag="WIE22_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ4127193.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAEGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYEWVLEPIYSI
                     SGKL"
     gene            complement(10731..11447)
                     /locus_tag="WIE22_001098"
     CDS             complement(10731..11447)
                     /locus_tag="WIE22_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ4127194.1"
                     /translation="MNRPKQPFFRPEVTVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            complement(11508..11867)
                     /locus_tag="WIE22_001099"
     CDS             complement(11508..11867)
                     /locus_tag="WIE22_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ4127195.1"
                     /translation="MKRKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAATGMGVAAYEYYQETQPKGTVTVGPISA
                     FAVDKSGNGYTDGTDYC"
     gene            complement(12287..13435)
                     /locus_tag="WIE22_001100"
                     /pseudo
     CDS             complement(12287..13435)
                     /locus_tag="WIE22_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            13688..13981
                     /locus_tag="WIE22_001101"
     CDS             13688..13981
                     /locus_tag="WIE22_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127196.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            13978..15021
                     /locus_tag="WIE22_001102"
     CDS             13978..15021
                     /locus_tag="WIE22_001102"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ4127197.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGIA
                     VADYFPSLRGVYGLSFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLLEQGVRVIDLSA
                     DFRIRDIPVWEHWYGMPHASPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETVLRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(14995..15243)
                     /locus_tag="WIE22_001103"
     CDS             complement(14995..15243)
                     /locus_tag="WIE22_001103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127198.1"
                     /translation="MIETGEMTESKRVEMCRKLLKPDKLDSRFCGNDGILDFCFRGIM
                     SQKPSEPKKPPLSDGIKKPQARCLRFAVCVSGEQGDCV"
BASE COUNT         3669 a         3618 c         3545 g         4518 t
ORIGIN      
        1 ctgtgtgcct tgcagtttgg tatcccagcc cgattggagg ttggctgatt ctgcaactac
       61 ccttgagggc agcgcggttt tcatgacttg ggtggtggtg tcgtgtgctt tgctgtagct
      121 gatgccgaga aatctgcgcc ttttttggct gtctagtttg tcgtagttga gctcttctac
      181 ggcatagagt gtcaatttcc gcccggcttc gatgttaatg ctgcctttgg gggcatcgaa
      241 tgcggtggcg taggcgtcga tgctgccacc agatttgatg tcgatgcctt gggatgcgct
      301 gaggtttact gcgtcgggct tggcgttttt gtgttctttg acttcggtaa tgtgtttgcg
      361 gttgtaccat ttgccggttt tgtagctgcg gcgttcaaag gtatagagtt cgctctgtct
      421 cgcatagtaa tattggtcac cgtaagattg aattttgatt ttgccgtttt ccgaactgat
      481 atccgtggtg ctgagcagga tgcgactgtt ctcattggca tacggtgcgc taatgctcac
      541 accggtttta cccgaaagtt ctgcagcaag cgtagggggt gtcttcagca cgcacgcgtc
      601 tgggcagtat atagggaatc tgaatattta cttgcataac aaatgccgtc tgaaaaattg
      661 tgagcttttc agacggcatt gagccgtaaa tcatggaacg cgtgcgtgct gaagcacaca
      721 ccttacgcat ggattttagg tttcatgcag gctacagttt gtttgagaat atgttttttg
      781 tatttcttta cacttttttt gatattcagg gtgagctatt aagaaatcag atattgcaat
      841 attaaagtaa tgatatgcta aattaatagg tataattaat tcttcaggat tgaaatcatc
      901 tccaactgaa aaataaatag aaggagcttt atcaatatac tcaaatccat ttacctcttc
      961 ataatctcca atccatctgg tagcatctat tttttcatat tgagcaccta ttactccaaa
     1021 atgtccggta gttgtaatat ttttaaaaca ctctaaaagc gtatctttag tttcaaattg
     1081 gtaaattgtt tcaaaaaaaa tcgaaataac aaaatggttg ttatttatag attttttatt
     1141 taagtttaga aaaatattat ttttcataat ttttccttta aattattctg ggtgaatgtt
     1201 tgtaattctt cctgtattta catcaaaata tgatctaaat ttgattccgt caaaggtttc
     1261 tgagaattga atgacatttt ttctttccga tattgattta gttctttcat tttgagcaat
     1321 tttagaggct tttgaatatc cttgtgaagc agcattttga gccatttgaa gtattttatc
     1381 atcagaaaat tttttaggat tataaacagt ttttatactt gaaatttcct taaatccacc
     1441 atcaggttta cctgtcctgt ctagtgtagg aatctcatat ttaattcggg taatgccttc
     1501 aatatcagtt tgggtttcag attttacgcg tcctcctcgt gaatttagtt ctgccataaa
     1561 attggtgcgg ttatgggctc ctttaatgcc ctgtttttga ctaaaaccat cagcatttgc
     1621 cagatgtctt tttaggtttg tatcgaaact gatgccttcc gggtatttga cattagtaat
     1681 aggtgtattt tgttttacaa accctcttgg tattggaatg aatctaggat taactcctga
     1741 aataagtgta taaccttggt cggacatcca ttcggataaa ggctttgtat tgcccgattg
     1801 aagccaagat ttagcagcag tattcaaatg ataagactgt aaagccaaat ctgcttgggt
     1861 gtaagatttg ctgaataatt tataccattc atcatcttta ccaattagac ctgcttccca
     1921 agatgaatga aggcttctac tatcgatcaa atgttgtttt ctgattgttc tacattcagt
     1981 actacgggat atatccgtac atattgcaat cgaagcagca agtcttttat cagcaacatt
     2041 ttgatacttt tttacagtat tttttctgca cagttgagga ttattctgtt tggcgcatgc
     2101 agtcatttcg ttatcaaatt ctctaccctc tttgtcgcta agctgattat ttttcaccgc
     2161 tacctcagcc gcattcgccg ccgcatttac atcgccgccg accacaccgc ttaccgtacc
     2221 ggcaaccagt ttgctgtatg ccaaaatctg ttcgcgttct ttagctgtca aagtgtcagg
     2281 atttttgccg tttgtcaaag cctccccgac tatctcgccc acagccgcac ctatcgcacc
     2341 atcctgacac ttgcccttat tcgccgccgc tgccgcacag cccgctatgg catgggcaat
     2401 cttgtggact atgtagtgct gatccaactg tttgatttta ctggctgctt ctccatgcgc
     2461 ggtattgacc aaagccgcaa ggatattcgc ttccagattg tctttcaggc tgccgccgtt
     2521 gacagcggta ttaatcagtg cggcactgcc cgcattggct aggttgacgg tcaggttgtt
     2581 gatccactgc ttatcgctga cattgttcag tgccgaagcg ccgattttgt cggctacgcc
     2641 tgcggtagcg gcggcaacca ccagattttt caccgtgctg cttctgccca gctctttcag
     2701 ggttttgccg acatcgcctt tgttgttgat gaacgatacg gaagcctggc tggccaaaga
     2761 ggcgaatgct gcatcggttg cggcggcggc cgcaccgttt aatcccaata cggctccggt
     2821 tcctgcgcct gaggtgacca cggtaacggc cagtgcgata atcgccgcac ctgcttcggt
     2881 taagccctcc tgtttgtagt cccatctgtc gtaagcaagc tgcacctgat tccagttgat
     2941 gtttttcgct acttggagct gtttcagata ggcatactcg ggctgtttgg acagcttttc
     3001 gatttcggtt ttcagattgc ctttcggaat gtcgacgata tagccgccgg gtgcggacaa
     3061 tttgggcgga gtagggcttt cgaagctggg cagttttagc gtttcgatag tgctgccgcg
     3121 tccggcctgt ttctgccata cggttgagtt ggtttctaat ttttcttccg actggatacg
     3181 gttcacaatg cctttgagga taattttcgc atcggcacgg gctttttcgc ctacacctgc
     3241 ctgaatgtcc gcaccggcca gcgtggtttt gaattcggta ccttcgagca cggtatccca
     3301 gcctgaacgg gtggctgcag tttgggcgac gacgcggaca ggcaatttgg tttcgttcag
     3361 ttcgttttta ctgtaattgc tcttgcctac cttgatgccg ataaagcggc ggcttttttg
     3421 gacatccaac tcgtgcttgt ggatgccttc ttctgccagc agttgcaact cttcacccgc
     3481 aaccagggta actttacctg caggggcatt gaagcgggtg gtattagctt cgatgttgcc
     3541 gcctgcctga agcgtgatac cgttggcggt cagctcgacg ggggctggca taatcaggtg
     3601 gtcgcgggtg ctggtaaact tggtttttct gatgattttg ccgcttttac ctttggtttt
     3661 taagaaggta taggcatcgt tttgtccagc ctccagtaca atatcactat gggctttgat
     3721 gtctatgctg cctgagggag ctttgatttc ggatgcaccg ataataatac gtgcatcatc
     3781 gagtgccgca gctgcatgaa tacttacccc cgtacgtccg gtcaaacgtg aaggcttgtt
     3841 cagagcagct ttgtcgtagt gactcttgta ggtgggcttg ccaatttcat attggtcggt
     3901 tatgccgtca atcagaatag cagccgcctc tgaatctgct gcctttggca atacgcctgc
     3961 ggcgtgaagg ttcagttttt tggaagcggt aatatcggaa ccgctgattt cgatgccttg
     4021 tgcggaaatc aagtcaatat tttgtgcaga aagcttggct tgcaggtatt ctttgccttt
     4081 gggtttttta cctttaactt ccttgttgat ggcttgaata tagaaagcga gacggtcgcg
     4141 ttcttcttgc agggttggaa tcagcttgct tttaggcgag ctttttttca actgcgcaat
     4201 ctgctgttcc aattctttgg atttttggtt gagttcagcc gctttttgtg taggaaaata
     4261 attgctgaat gagttgttta cggcttcgat attcaacttg cctttggtgg tggcgacaac
     4321 caagttttta ccggctgtaa ttttagaacc tcttaaatct gtttctcctg taaccagacg
     4381 gatattgcct tttgcttcca atgaggaaac ttgagcacta ggcgcacctg catttcctcc
     4441 ttttgcagac aacagcaatt ttccgcctgt tttgatgctc aggtcggtat gcgcactgat
     4501 gcggttggca ggttcgatgg ttaatgtgcc gctacctgct tcaatattca gccgtccggc
     4561 caatggtttt aattcggcat tatcttccaa agtcttggtc gaaacggtac tccaattgat
     4621 gttgccgcgc ttgactaggg cggtaccggc agtaaggttg attgcacccg ctctcagcgt
     4681 ggtgttgtcg gcaatttggg aatagcgcgc attgagtgcc aatacaccgt tagccaccag
     4741 cttgttggca gaaggcagtt tgtcgttttg ccaaatctgg ctgccggtaa tgcttagatg
     4801 acggtgtgcg taggcatcta cttggttgag cgttacccgc tcgtgttgtg cattaagatg
     4861 cgtattatgg gtagactcca gcttggtatt ttctatgctc aatgcccggt cggaatgaat
     4921 gttcaatgcc ccgcttttgg catggacgtt aaggtttttt aagtcggcat taccaccgtt
     4981 gtttttgatg ctgatgtgtt ttccgttgat tgaattgcgt tgttttccgt caccaagctg
     5041 aataccgttg ccggcaacca acgtaatatc tcctgaagat gaagtgatat tggtattgtc
     5101 tgctttcaga cggcctttac caaccgatcc tgcattgaca tcggccttgg ctgtcagggt
     5161 attgtgaccg gtaaagtcgg cattaccgtt ggccaataag gatacatgac cgtctgcaga
     5221 aacagcatga aggccgtctg aaacgatatt gccctgcaat gcggtggttt cgagagcctt
     5281 ggctgcgttc agcttggtat tgcgaagctg aatattgcct ttggctgcct gaatgtgcag
     5341 attacccgag ttggtacgca gattggtatt ggtaacattc agcgagcctg cctccacacc
     5401 catgtctttt gaggcagtga gggttttact ggtgccggta atatgggcag cgttatccga
     5461 tttcaaatgg atgctggcgg cagacaaatc tttatcaaca ttcaaattca gatctttacc
     5521 tgtatgaaca tacagattgc cgggagtatt cagattggta gttttggcag taatgttatc
     5581 gtctgccagt aaagcaagct gcttgccgcc cttgatactg cctccgttta agcggatatc
     5641 ggaggtaact gttgaagctt ccaaagaaag cggtttgcct gcttcgatgt gtgcggtgtc
     5701 tttggcatct attacggcgg aactgctgat ggtgccgttg gataatacgg taacatctgc
     5761 cccggtaatg cgtgtgttat tgcctaattc ggcgttgcct ttgctggaac tgtatacggt
     5821 agtgccagtc tgaatactgg cctccttgat gacggtacgg ccgccggtcg acagattggt
     5881 cgaaccttta gcattgttca catttgcttt gctttcgata accaaattac ggccggcatt
     5941 taataccgta gcagcagggc ggttgccgtt gttttgaacg accgctccgt tacgcaaacc
     6001 gatgtcctcc ccggtatcaa taaccaataa gcctttgctc tctatccggc cgccattgga
     6061 gataaatgcg cctgctgcgc ctgtttcggt ggtttcgatg gagagataag tcggtgatgc
     6121 ttcagtgctg tcggcagtgg tggcgatgcg gccgctgttt tcaatacggc cggaggaagt
     6181 aacgatcaat tgcttggccg cttcgagtgt gcccgcattt ttgacaccca cgcctttttc
     6241 attggcaatc agggtaatgc tgtcggcgta cataccaccc aatgcggcag tatcgagggc
     6301 aacggtcggt ttcgtacccg ctgccgtacc tgcgctgatt tcaccgctgg cgtaatctac
     6361 tttctgtgcg ccggtcgaaa ccgccaggtt tttaccctgc aatttcccct gcaaagcaac
     6421 cgcacgggca agcacttcgg tgtaatcggc tccgcctttg tcgttccaac ctgacgtacc
     6481 gacggtcaat gtgccttggc gcacgtcaaa tcccgtcaag gcaccgtctt tgccgatttg
     6541 aggcgtaccg gtggttaaga taccgcggcc gacgttttta aagccgccgc cgttcacggt
     6601 aatgccgttg gggttggcga taatcacatc ggccttttga ccacctacgg taacgatgcc
     6661 attgagtttg ctggccgcac cgcgcacctc gttcaaaatc aactgcgcac tgcctttggc
     6721 cagaaacggg ttgttgttgc gatcgttgtt cagtacagca cctttagcgt cgacatcaaa
     6781 ctgggtatag cggttgtggc tcaatccacg ggcattagga gtttggatgt tgaccaacgg
     6841 cgcaccggta ttggttttaa gaataacggc ctgctggttt ttgggcgcgg ttttatcggt
     6901 aatgatttgt gcgtgggcag gcaataccat actgacggaa accaaagagc agaccaaggt
     6961 tttaagcgtg gttttgagtt tactgtaaag gccgtctgaa atttgtggcg aagccggagc
     7021 ggaagttcct gcctgtttgc cttttccctg actgttggcg gtttcggcaa ctgcaaccat
     7081 agtgttgtgc tttttgctga aaacggtacg atgcagacct ttgttcatat ttatcccttt
     7141 aaatgatgtt tgtaatcgct tcagacggcc tgtaaatgat gaaagactgt ctgaatgtcg
     7201 gtaaaaaatg caggagaggg ttagaaactg taattcaaat tgaagccgta aacggtgttg
     7261 gccgtctgaa aaccttcggg cttgtgcagc ggcttgccgg caaataagtc ataagcaaat
     7321 ataccgccta ctttatggct gcctctgaag ccggccactg cacccgccag ccgcttgccc
     7381 gaggcatatt gggcactttc gcccgatacc cgaccatagt ccgcgccgag atagaattga
     7441 tggcgcggat ggaaatgcca gcccaaagtg ttctgccagt agaaaccacg ctctccggaa
     7501 aggctctgtt caccgtcaaa tcctcgaacg gtgtagcggc tgccgataga caacttgtct
     7561 tgggcaacca aaggcgtttt gttccattgt gcatggatag cggtttcgta ggaaaactgc
     7621 tgtttgccca acataaacgg ggccgctgca tccaatccgg cggttatgat tttcatacgg
     7681 gatgtgcctg gaatagtacc gccgccgttt tcttcaggtg cgggcatact ttggcgcata
     7741 ccggtacccc gtttgtaaga taacttgccg tccagttgcc agcggttgag gtaagcacgg
     7801 tggcgcaatt cggcttccca gcctgcagag cggcggcgtt gcacttcgat ttcggcatcg
     7861 tcgatgtatt tataggtttg gcgtgtccat aatttcattc cgactgaagt tttatgaaac
     7921 ctgttacgcc aaagcatgcg ctcggcggcc aggctgctct gatattgttt gccgttgtaa
     7981 tcgtaattga cggaatagcc ttcggttgct tcgtggtaac gatgtccatt gtgattaaaa
     8041 gaaaacagcc atttttttac gggcaccgaa taatgcacgc tgtaacttct ggatccgctt
     8101 gcagtttccg taccggtggc atcagtcaag tccgttttgt gcgccaaacc gcgtccatat
     8161 gaaacataaa acaaatcgct taagcccaaa gggttatcga acgataaagc gacatttcct
     8221 tgatatttgc cggtcgtttt gccgcccgca tcatctatac cgatactgaa ccgtatgggt
     8281 ttattctgct gccatttgat ctgtaaatcg cttttgcctt cttcttcgga cggtataatc
     8341 tgaatatctg ttttaacact cgacaaacga cgcaggtttt ccaagccctg ctctacatcg
     8401 cgaagattga gaattttgtt cctatataag ggaaatttgt tattgaatgc actaatactg
     8461 ccctcggcag acttcccatc ccgtttttct tcatagcgga tatcccctat ttcgcctgct
     8521 gatacccgta atttcagaat tcccgaatcc atattctgtg gttggataat agcttgggaa
     8581 gtgaggtagc cacgcacaat cagtatctgt tgcgcggctt tttgtagcct gctcaaatta
     8641 ttggaaccta aacacatccc agttttaaaa gctgtttctt tcatgagcac agaaggaaga
     8701 aaagaaaatt tgcgcgccgt cttatcatct aaactaatgt aatttacccg agtacacggt
     8761 gtttcatctt cactcaggac ataattgttc ttctccaatg gttgctcgaa acggacattt
     8821 gcatcagtta acaattcagc atctatgtgc tgctgacgct gcatggaacg gataagttct
     8881 gcatcgtttt catcggcagc taaggtttta aggggtatga cagccaggat aaccaacaga
     8941 catggagcag gaaaaaattt catgacatta atattatttt agcaatattt actattctgt
     9001 cataaattta aaagtattta cagttataga atgagacctt tgcaaaattc cccaaaatcc
     9061 ccaaaattcc caccaagaca tttaggggat tttggggaat tttgcaaagg tctcggacag
     9121 tattttgaac gcagtgcgcg taaatccgta tggaaaccat gaaatcccgc cacagccgcc
     9181 agacatgcca agccgcattc tgatatttct gtttgcagga taacaggcag ctttttcttt
     9241 aagcccaaag acaggttttg cagatggggc atagatttcc tttttgaaaa atagggatta
     9301 ggaagttgga tgtattttag aaaggccgcc tgaaaaggtt tcagacgacc ttttgcgact
     9361 agctgctatt ttatttaaag cttttctcta acaaacgagc taatattttt cctgtaataa
     9421 aacagataaa aaacagcatc caatacgtca gattggaaaa atcggtcgta tagagaatca
     9481 acatataaag aagcagcatg atgccgagtg cgatgaattg ataatgtttg gcaaacatca
     9541 tgacctcctc aactattaag gcaaaccgcc tgaatattct cgttcaatcg tttcggcaat
     9601 ttccctataa cgtcgatacc atgaccagtc gaaattttca atggcatggc tcgcaaacgt
     9661 accaaattca ggcatcccta tgcggctacc tgctaaagct ccgattgtag ctccccaacc
     9721 aggcggatta ctgaacgtat tgtttggcaa tcctaaagat ttatcaggat ttcccgtatc
     9781 gttaagccct gcagattcgc aaatttcgca ataatatgag ctttgttacg cattacctga
     9841 gcctccgacc caagtcattt catgaacaca tatagtggat taaatttaaa tcaggacaag
     9901 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgct gtactggttt
     9961 aaatttaatc cactataaaa ctctcatttt gaaactcctt gtatcgttaa tcaaacaatc
    10021 aaaagggcag atgccctatc cttgctttta cagacggagt gcctgtaaaa ggggatggtt
    10081 tcaggcagtt ttgaagtttg tgtttttata tattgtcttc tggtcgtctg aaaaggtttc
    10141 agacaacttc tttatctttc agcctcaagt cttacagttt gcccgatata ctataaatcg
    10201 gctccaatac ccattcatac aatcgccgtt tctcgtgcag gatgtctgct tccaacgtca
    10261 tgccgatttg cagcggtttt tcctcaccgt atgcagtgat ggttggtttg tcgggtttta
    10321 ttttcacgag ataaacaggt tcgttgctct tcgccaaatc ggaggatacc atgcccaatc
    10381 ccgacaattc ctgtctgccc agtgccgttt ttgctactga tacgacactg ccggaagcaa
    10441 gcccgaattt ttgataggga tatgcctgat aacgtaggac aaccttgtct ttcggcttga
    10501 taaagccttc tgcactgctg gggatatata gatgggcata tagctcggta cgttcgggaa
    10561 caatgctcaa gagcagtttg gaaggatcaa cctgctgtcc gacttcgacg ttcggtattg
    10621 ctatataacc cgaccgtcct gcacggatga tttgttcaga gcgcatttca aaatccaaaa
    10681 cttcttgaga aatatcggca atggtgcgtt caagccagct ttgttctgtc tcatgccgct
    10741 tgggggaggc tggccaatgt cagattctgc gtgcggattt cctgaagcag cccgacttct
    10801 tctcggcggt aggcatcaag tttggctttc tgctctaaaa gctctgcctt gacattcatc
    10861 atttcttgtt ttggcactgc atcattggcg gataggaaac gatatttctg caacatttct
    10921 tccgcaagtc taatgcgcct tttctgaccg tctatctgtt gcgaaatatg gagttcctgg
    10981 ttttccaaac gttcgacagt tgctttaagg ctgcgcgttt cattcccgtg tatcagcttc
    11041 agacgaccca gttcctgttc tgccaacgtt ttcttcaaaa ctgcctccgt tttcaactgc
    11101 tgctgcacgc tacctcctgc gccgaaacgt gaggtcgaaa gcgcaaatag cttgtcgcca
    11161 gccttaacct tttctccatc ttccacgaat ttcgctgtaa ttgtccccgt atccggtgca
    11221 tacaccctga ttacgcccga tgcaggtaaa atttgtccct ccactgttgt ctttcgcgta
    11281 tagttaccaa atatcaaaaa caggataatc aataacgcag atatcgatgc aaatgtcgtc
    11341 catagggaaa atgacaacgg tcgtgtcaga atcactttac ccgtcaggct ggtttgccgg
    11401 gcaacagtga cttcagggcg gaagaagggt tgcttgggtc tattcataaa attgaagtta
    11461 agaaagtttc agacgacccc aagagatcgt ctgaacaatg agaaatatca gcagtaatct
    11521 gtaccgtcag tgtagccgtt tcctgatttg tccacagcaa aagcagaaat cggaccgacc
    11581 gttactgtcc ctttgggctg agtttcttgg taatactcat atgctgcaac acccatacca
    11641 gttgcagcac caacagcagc accaactgtg ccaccgagaa taatagccgg tccggcagga
    11701 ataccaccag aaactatacc gacacccaga ccaatacctg caccaattga tcctcctatt
    11761 tgtgcaccat ttgctcctcc ttcaaggatt ctctccccta agcctccgcc ataaattagc
    11821 tccagattct caatggatag ttcaatgatg ttatttttcc ttttcaaaaa aattagacat
    11881 agaatattga gtcaatacac ccaatatgga aaaaataata aaagcacgaa tataaatcat
    11941 aaaattaaca tcatctcctt ggtagctttc ataaataaaa acgattggta ttgatataca
    12001 aattaaaata tacaggtact ttgcaagcac cagtaactta tacacaaatt ttgatttcat
    12061 agcctagtct ttcattggtt ggtattccta aaaattcggc taacaaaaac atatggcaga
    12121 tatattgaaa aaaaattcaa agtaccctga ataaaattca aattccaact atatttgtta
    12181 atgtagtcga gaagaaacat atctgataaa aaatatagca attgataaca agctattact
    12241 aatattacga aaaatgtaaa ttgcttccag tttttcatag aatccctcac aaaatttcca
    12301 gaaaatctaa ctctatcaac tgataaatca acttcctaac ttcttcatat tttccctgat
    12361 tgaagttaac cagtagattt ttcaacaata acggttcatt cttaccgatg tgttctaaca
    12421 cttttttccc caactcatct acgcttatct tcatcccatt cccaatcaaa tatccctttt
    12481 ccaacgtatc caaattattg gcatttaatc tcaacctgac gtcgtctgaa agcggagtag
    12541 cgttgggatt cgcgaactgt tcgagatgaa aagcggtatc ggtacgttct ttgccgagaa
    12601 agtcttcact gaaggcttca taattgacgg ggtcggcaat catggcagca atttgtgtgg
    12661 cagtatcgtt gatacgcgtc ctatcttact cccagtctga gaaactgtgg cgcagacttt
    12721 ctatggtggg gaatttcttc attagccact cgaggtaatt atagccgttg ggcggaaagg
    12781 taccgacagc gaggtggaag gtttcacagc cgagcgggat aggtctgtgc caccaaccgc
    12841 gcgggatgta gaggacatca cctgcttcaa ggataatatc catatcgata tgttcaggaa
    12901 tggaaatatc agtatcttta gtctgttgca tatacaatgg catagggaaa tcaggggcag
    12961 taagttgcca acgtttcttg ccgaaaagct ggatggcata cacatcgcgg gtgtcccaat
    13021 ggtttttata agattcgtcg ctgccaaaag caagataccc actaacaatg gtatgtgcgc
    13081 cggcaaggcg aggcaacgct gtactggttt aaatttaatc cactataata tggttataga
    13141 gcaacgacgc accattcttc atatgttcgt agataacgga tttaataaaa cggtagcgag
    13201 ttttgcccaa atcgtcgaaa ctttcgacgt attcttcttt aggaacaatt gcaccttttt
    13261 tacgcagatg aaacagcggt gcggttgggt ctgctcgttg gtatatctcg ttgatatctt
    13321 tccaagatgc ggcttcgaga ttccgaaccg ctcctttaaa gagcttgggc ttttgataca
    13381 gataagtctg tcggaacgtt ttaggactaa tgccgaagtc gagatggatg ctcattactt
    13441 ccccttactc agaaaatatt taaaatttat aattttacat atatttacaa atattaaagt
    13501 ttttttgtgt gtgcgtcaag gaattgttga caattttagt taaaaatttg tctccaaacg
    13561 gaaaaaagcg gtttgttttt tgttgttaac atttttattg taattaaata aaaggtcgtc
    13621 tgaaaacggt tttcagacga ccttttgcta taatcgggct tcatcgcccc gttcggtttg
    13681 gaaccttatg aaaaccctcg tcctcctcct gctttccttc tccacgacca ccgctttcgc
    13741 cgcatacggt ttgggtttgg ggcaggcacc gaaatatcct gccgactttc gcgcctacgg
    13801 ttatgtttat tccggacggc agggctgggt tttaaaaaca gaggcggatg ccattaaatt
    13861 agacacgctt ttcaaacgct ttgtgtaccg tccttccgcc gccaatcaaa accccgtcgg
    13921 acaagccgtc ggacagcgtt cggacggcat acccgccaac cacacaaagg aaaaaccatg
    13981 agcaaaaaaa tcaaggccgg cattgtcggc gcgacgggct acacaggcgt ggaactgctg
    14041 cgcctgcttg ccgcccatcc cgatgtcgaa gtcgctgccg taaccagccg cagcgaagcg
    14101 ggaatcgcag ttgccgatta ctttccgagt ctgcgcggcg tgtacggcct ctccttccaa
    14161 acgcccgacg aggcaggttt ggaacaatgc gacatcgtct tcttcgccac gcccaacggc
    14221 atcgccatga aagacgcgcc gcgccttttg gaacagggcg tgcgcgtcat cgacctttcc
    14281 gccgacttcc gcatacgcga catcccggta tgggaacact ggtacggtat gccccacgcc
    14341 tcccccggcc tcgtttccca agccgtgtac ggattgagcg aactcaaccg cgaagccgtc
    14401 gcacaggcgc gcctcgtcgc caaccccggc tgctacccga cctgcgtttc cctgccgctc
    14461 gtgccgctgt tgcggcaatg ccgtctgaag cccggtatgc cgctgattgc cgactgcaaa
    14521 tccggcgtgt ccggcgcggg caggaaaggc aatgtcggtt cgctgttgtg cgaagccggc
    14581 gacaacttca aagcctacgg catagccgga caccgccacc tgcccgaaat caggcagacc
    14641 atcgccgggc ttcaggacgg catcgccgaa ggattcgtgt tcacgccgca cctcgcgccg
    14701 atgatacgcg gtatgcacgc caccgtttac ctccaccttt cagacggcat ctgccccgaa
    14761 accgtcctgc gcgactatta ccgcgacagc ctgttcgtgg acatcctgcc cgccggttcg
    14821 acaccagaaa cccgcagcgt gcgcggcgca aacctctgcc gcatcagcat ccaacaggcg
    14881 gcgcaatccg atgtgtgggt cgtcctttcc gtcatcgaca acctcgtcaa aggcgcggcg
    14941 ggtcaggcag tccaaaatat gaacatcatg ttcggactga aggaaacaca cggcttagac
    15001 gcaatccccc tgctcccctg aaacgcaaac ggcaaaccgc aggcatcgtg cctgcggttt
    15061 tttgatgcca tctgaaagcg gcggtttttt aggttcagac ggcttttggc tcattattcc
    15121 gcgaaaacaa aaatctaaaa tcccgtcatt cccgcaaaag cgggaatcta gtttatccgg
    15181 cttcagcaat ttccgacaca tttccacacg cttcgattcc gtcatttctc cggtttcaat
    15241 catttccgat aacaccgtgg tttttcattt ctagattccc gcctgcgcgg gaatgacggc
    15301 ggagggcttg ccgtttttcc tggtaaatac ctgcaattta aaatcccatc
//
LOCUS       DAWHXS010000046        15182 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWHXS010000046 DAWHXS010000000
VERSION     DAWHXS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.046"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(3..323)
                     /locus_tag="WIE22_001104"
     CDS             complement(3..323)
                     /locus_tag="WIE22_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HEZ4127199.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLPARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARSI"
     gene            519..977
                     /locus_tag="WIE22_001105"
                     /pseudo
     CDS             519..977
                     /locus_tag="WIE22_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            877..1245
                     /locus_tag="WIE22_001106"
     CDS             877..1245
                     /locus_tag="WIE22_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ4127200.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDNLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLVGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1314..2852
                     /gene="lnt"
                     /locus_tag="WIE22_001107"
     CDS             1314..2852
                     /gene="lnt"
                     /locus_tag="WIE22_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ4127201.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFSLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3116..3979
                     /gene="rpoH"
                     /locus_tag="WIE22_001108"
     CDS             3116..3979
                     /gene="rpoH"
                     /locus_tag="WIE22_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ4127202.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4038..4184)
                     /locus_tag="WIE22_001109"
     CDS             complement(4038..4184)
                     /locus_tag="WIE22_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127203.1"
                     /translation="MLIHYINSANLSEKQLMRQCLVHVCFLISAIAMVWTDKIVYGTT
                     HKPH"
     gene            4463..5347
                     /locus_tag="WIE22_001110"
     CDS             4463..5347
                     /locus_tag="WIE22_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ4127204.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIDEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5431..5946)
                     /locus_tag="WIE22_001111"
     CDS             complement(5431..5946)
                     /locus_tag="WIE22_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ4127205.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6022..6441)
                     /locus_tag="WIE22_001112"
     CDS             complement(6022..6441)
                     /locus_tag="WIE22_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127206.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6562..7560)
                     /locus_tag="WIE22_001113"
     CDS             complement(6562..7560)
                     /locus_tag="WIE22_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ4127207.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAVLKQGQGVQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            complement(7562..8041)
                     /locus_tag="WIE22_001114"
     CDS             complement(7562..8041)
                     /locus_tag="WIE22_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127208.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALR
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8093..8872)
                     /gene="pgeF"
                     /locus_tag="WIE22_001115"
     CDS             complement(8093..8872)
                     /gene="pgeF"
                     /locus_tag="WIE22_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ4127209.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALGGTPDADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9039..9152
                     /locus_tag="WIE22_001116"
                     /pseudo
     CDS             9039..9152
                     /locus_tag="WIE22_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9431..10378
                     /locus_tag="WIE22_001117"
     CDS             9431..10378
                     /locus_tag="WIE22_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ4127210.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(10531..11655)
                     /gene="rluD"
                     /locus_tag="WIE22_001118"
     CDS             complement(10531..11655)
                     /gene="rluD"
                     /locus_tag="WIE22_001118"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ4127211.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11654..12457
                     /locus_tag="WIE22_001119"
     CDS             11654..12457
                     /locus_tag="WIE22_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ4127212.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12515..14734)
                     /locus_tag="WIE22_001120"
     CDS             complement(12515..14734)
                     /locus_tag="WIE22_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ4127213.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3685 a         4567 c         3712 g         3218 t
ORIGIN      
        1 cactatatcg accttgccgt cgggcaggat gtaccacgcc aaatccccgc ctgaagcacg
       61 gtgtcggact gcatttcgat tcggctatac gccttttcac gcaggctgta attgaatttc
      121 cacaaaccgc cgaacactgc cggcggacgc gcgaccagca tcaggaacgc cgcaatcgcc
      181 aaggcttccg ccgccccgtc caacacgccg gcgggagaaa ccagcagcaa aggcattccc
      241 aaacgtgcgg gtaaagtggt cgaaaccacg ctcagaaaca acagtgcgcc acccggcagg
      301 aacagactgc ccatccaatc cacgctaacg attctccctt aaaattcatg aaattaaaat
      361 cgaaagactg taacacggca ttttcttttc acgaaacttt acttcacgtc cgccacagcg
      421 ttcagacggc attttcctgt ttcatgccgc ctgaaaccgc ccaaatccgc gttctcctat
      481 ataatgtcgg actttgcgcc gcatttcagg attctttaat ggcatttgcc tcactattta
      541 ccctgccgga cgacatcacc gccgttttgg acgatgttgc cctgatgacc aaagccgccg
      601 caaagaaaac cgccggcgtg gtcggcgacg atttggcacc caatgccaac cgggttacgg
      661 gcgtatcggc agaacgcgag ttgccgattg tttggtcgat ggcgaaaggt tctttttcca
      721 acaagccggt tttggtgcct gcggccctgc tgctttccgc cttcctgccg caactcatta
      781 cgccgctttt gacggcaggc ggcatttatc tgtgctttga cggcgtagaa aagctgctgc
      841 acaagtttct gcaccgacat gacgcgcatg ctcgtaatgg cggcaatcgg catcggcatg
      901 actgttttgg tgtacggaat cgttgccgtc atcgtcaaac tcgacaactt aggcatgctg
      961 ctgatgcgcc gcccacaaac cttttcgcgc agtttggggc aaatgctgac tgcctttatg
     1021 ccttgcttta tgcgcggttt gagcgtggtc ggcacgcttg ccatgtttct tgtcggcggc
     1081 gtactggtcg cccacaacct cggccttctg cacgatttcc tgcacgcgca acactgggac
     1141 gctggctggg cggagtactt cgccaacctc gttgtcgggc tgctttccgg ttcgattgcc
     1201 tgcgcccccg ccttgccgct gatgaatcgt ttcggcaggc attgatttct ttttcaaata
     1261 ccgatgccgt ttgaaagatg ttcagacggt atcttccgaa cagacagatg aatatgtttt
     1321 ccaaactgga caaatactgg cagcaccccg ccctctactg gcctttgctc atcctttttg
     1381 ccgccgccac cccctttacc ttcgcaccct actaccactt ttggctgatg cccttgattt
     1441 tcggtgcctt cgtccgcctc atcgaactgc gtccgcgttt tgctgtctct tccgcctacc
     1501 tgttcagcct gaccgcatac acgacacagt tctactggat acacaccgcc ctgcacgacg
     1561 tttccggcct gcccgacctc tatgccgtac cgctgacttt cctactcccc gcctaccttg
     1621 ccctttatcc ggcactgtgt ttctggctgt ggaaaaaatt taccctgcct cggggcataa
     1681 aaatcggttt ggtactgccc atcctgtgga cactgaccga gtttgcccgc gaacgtttcc
     1741 tgaccggatt cggctggggc gcaatcggct actcccaaat caccccggac agcccgctcg
     1801 ccggctttgc cccattgggc ggcatccaca tggttacact ggcaaccgcc tttctcggtg
     1861 tctggctggt tttggcgagt gataacaccg cacgttcggg caaacgcctg cttccaatta
     1921 tcctgattgc cgccctgctt gccgcaggct acaccgcccg acaaaccgac ttcacccgcc
     1981 ccgacggcag ccgcagcacc gtcgccctgc ttcaaggcaa catcgaccaa accctcaaat
     2041 ggcgtgaaga ccaagtcatc ccgaccatac agaaatatta cgaacaggtc ggcaaaacca
     2101 ctgccgacat cgtcatcctg cccgaaaccg ccatccccgt tatgcgccaa aacctgccgg
     2161 aaaacatact ggcaaaattt gccgaacagg cgcaaaacaa cggcagcgcg ctcgccgtcg
     2221 gcatcagcca atacacttcg gacggcaacg gttacgaaaa cgccgtcatc aacctgaccg
     2281 gttatcagga aaacaatcag gacggcatcc cctactacgc caaaaaccac ctcgtcccct
     2341 tcggcgaata caaaccgctg cccttcctga ccacgccgct ttacaaaatg atgaatatgc
     2401 ccctttccga cttccgcaaa ggcggcggta agcaatccgc cctgctgatg aaaaaccaaa
     2461 aaatcgcctt caacatctgt tacgaagacg gattcggcga cgaactgatt gccgccgcca
     2521 aagatgccac cctgcttgcc aatgccagca atatggcgtg gtacggaaaa tccaacgcca
     2581 tgtaccagca cctccaacaa tcgcaggcgc gggctatgga actcggacgc tatatggtcc
     2641 gtgccaccaa caccggcgca accgccatca tctcccccaa aggcaacatc atcgcccaag
     2701 cccaacccga tacggaaacc gtattggaag gacacatcaa aggctatgtc ggcgaaacgc
     2761 cctatatgaa aaccggcagt tcatggtggt tgatgggcat attgacccta gccgcactga
     2821 ttcttttcat cttccgaaac aaagaacact gatacctgca cagcccgtcc ggacgggctg
     2881 tcttttttca aaacggctat ttacatacaa ttacgtatgt aatataattc tgaaatcgaa
     2941 ttgagccatc aggtttacaa aatcaatcaa acctgcctta caaaattttc ctgtctttca
     3001 aaccttagcg catatctcct gccaaacata caaaatacaa gccaagcagt gatatactgc
     3061 atcccaacac agcaaaccgc cgatacgcag tttcagccga aaggaaatgc cccaaatgaa
     3121 taacgctttc gcattacccg ccatccaaag cggcaacggc agcctcgaac aatacatcca
     3181 caccgtcaac agcatcccga tgctgtccca ggaagaagaa acccgcctcg ccgaacgccg
     3241 gataaagggc gacctcaacg ccgccaaaca actcatcctg tcccacctgc gcgtcgtcgt
     3301 ttccatcgcg cgcggctatg acggctacgg gctgaatcag gcagacctga ttcaggaagg
     3361 caatatcgga ctgatgaagg cggtcaaacg ctacgagccc ggtagaggcg cgcgtctgtt
     3421 ttcatttgcc gtacactgga ttaaagccga aatccacgag tttattttga gaaactggcg
     3481 tttggttcgc gttgccacca ccaaaccgca acgcaagctg tttttcaatt tacgcagtat
     3541 gcgtaaaaac ctcaacgccc tgtctcccaa agaggcacaa gacatcgccg acgatttggg
     3601 tgtcaaactt tccgaagttc tggaaatgga acagcgcatg acgggacacg acatcgccat
     3661 catggcagac aacagcgacg acgaggacag tttcgccccc atcgactggc ttgccgacca
     3721 cgattccgaa ccgagccggc aactatcgaa acaggcgcat tacgccctgc aaacagaagg
     3781 tttacaaaac gctttggcgc aactggatga caggagccgc cgtattgtgg aaagccgctg
     3841 gcttcaagac gacggcgggc tgacgctgca ccagcttgcc gccgaatacg gcgtatctgc
     3901 cgaacgcatc cgccaaatcg aagcaaaagc catgcagaaa ctgcgtggtt tcctgaccga
     3961 agaagccgaa gcggtttgaa atatacgcaa taaaataccc gacaaattgt tttgtcgggt
     4021 attttatttg gtaaacatca atgcggtttg tgcgtcgtgc cgtacacgat tttatccgtc
     4081 caaaccatcg caatcgccga aatcaaaaag cagacatgca ccagacactg ccgcatcagc
     4141 tgcttttccg acagattggc ggaattgata tagtggatta acaaaaatca ggacaaggcg
     4201 gcgaagccgc agacagtaca aatcgtacgg aaccgattca cttggtgctt cagcacctta
     4261 gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggttttgg ttaatccact
     4321 atacatttcc cttccttgca gcaacaaaag agcgtgattc caatctgccg ctgcaaaacc
     4381 ttcaattttt cacacccgaa aacgcttgcc gccatacaga tgtgatatat tgattccatt
     4441 ttaacgggct tcggagaacg acatgagcag cggaaatatc cacatccaca gcatgaccgg
     4501 ctttgccaac gcggcggcag agtgcggcag caaacgcatc aacctcgaca tccgcgccgt
     4561 caaccaccgc tttttggaca ttcaaatcag gatgccggat gatttgcgct atttggaaag
     4621 cggtatccgc gagaaaattt catcccatat cgcgcgcggc aaagtcgaat gcaaaatcca
     4681 aattcaagat acggaaaaca gcagacagtc cttggaactc aactcggatt tggtgaacca
     4741 gcttgccgaa atcaacaaaa acctccgcaa gcatcacgat ttggcaaaac tgggcgttgc
     4801 cgacatcctg cgtttccccg gcgtattggc aagccaaagg gaaaatacgg aggaattggc
     4861 aaaaagcatt accgaactga ccgaaaaagc gttaaaagac tttactgccg cacgccggcg
     4921 cgaagggaaa aaattgggcg agcacctgtt acaacgcctc gaaagcatgg aagaaatcat
     4981 agatgcattg agcgaactct ttcctaccct gctggaaaca cataaagaaa aaatccgcgc
     5041 ccgcctcgtc gaagcagtcg gcgacatcaa taatgacagg ctgcaacagg aatttgccct
     5101 ctttattcaa aaatccgaca tcgacgaaga attcagccgc ctgcgcacgc atatcgacga
     5161 agtacgccgc atcgttaccg aacacaaagg cagcagcgga aaacgcttgg atttcctgat
     5221 gcaggaatta aaccgcgaag ccaacacttt gggcagcaaa tccattgccg ccgaatgcac
     5281 ccaagcctcg gtcgaactga aagtcttaat cgagcagatg cgggaacaag tgcagaatat
     5341 cgaataatcg gtttccccga taaccaaatg ccgtctgaaa gccctttcag acggcatttt
     5401 ttcaggttga aacctttgcg gtataaaggc ttaaggcttt cctttgaccg ccactaatct
     5461 tgttcctgca agaaaatcat acagaaactg ccgatcggga ttcagcaggg cgaaccccca
     5521 cggtaaaatc agccaaatca atgccgcgcc ggccgcgccc ttgggcggaa tgccgaggaa
     5581 gtggcgcaat ccggcatagg ctaacatagg gataaatacg ataaatatgc acgcccaaat
     5641 aaagcgcagg cgcagcaggt acaaagacgg ctgtatgccg ttaaggtcgc acaagccgat
     5701 tttccatgtc ctcatcgcca aggtctgccc ctgaccatgc cagttggcgc ggaaataaag
     5761 ccaccatgct cccattatca ggatacttgt taccaatgca gaaaccgcga tagaaacggg
     5821 attcagaaaa atggcggcaa taccggcaaa caatgctgcc aaacaggttg ccgcaccgac
     5881 cagcagcatt tcgtacagca tggcggcaaa gcggcgtttg agcggggcgg ttttcagtgt
     5941 catcatcttt tcaatcagaa tatgccgtct gaaacgccaa caggcttcag acggcattta
     6001 tgcaaagatg ccttgcggcg gttaacggtg cggacgtttc tgaccgcgcg actgttttgc
     6061 cgccatttga ttgatttcct gacgtgtcgg gcgttttttg aacatagcgt gcgcctcgtc
     6121 caccgaacat ttttgcgaca ggcagatcag ggtaatcgca tctttctggt gttgtccgcc
     6181 gctgtggtac atacggcgca ggtttgcctc gttgattctg cctttttcgg gatcgatgtc
     6241 gaattttttc aggcgttcga taaactggcg ttgctgtcgg cgggatgtcc aaacaaaacc
     6301 gcccgcagcg gcagcgacaa tgacggctgc aagcaggatg gcaagcggcc aggaatcgac
     6361 aatcaatacg ataatgaggc ttaaaacggc ggtggtcagt aaaatcaggc cgcccaagcg
     6421 cagtttggtc ttattgtcca tagggttaat gttcctttat tgaggtggtc gtgccgtctg
     6481 aaatgttttc ggacggattg gtgaatactt ttcaggatta acggtatttt acagtatttt
     6541 cggattttgg ggattaccgc tttattccgc cagcgacacg acaagccgtt tgaataccgt
     6601 ccatgcgtcg ccgtcttccg cgcctttgat gattcggtcg atttgggcgc aggttttgag
     6661 cgcgtcaagc aggcggacga cggaaatccg cttgaccgca agcggcgcga gcgtctgctt
     6721 gtcgccccaa agtctgagac tgttgcgtac ggattggaca ccctgcccct gtttcaggac
     6781 agcggcaagc cgaatcagcg tccgcacgtc ttcggcaacc gcccacagaa gcagtaccgg
     6841 ttcttcgcct tcctcctgca atccgtccaa caacctgcat acgcgcggga catcgccctt
     6901 catccatgcg ccggcaagct gaaaaacgtc aaagcgggca acattggcaa cggcggtttg
     6961 cgcctcatcg atattgacgg tatgcccttt cggatacagc agcgcgagct tgtcgatttc
     7021 ctgacgtgcc gccaacagat tgccttccac gcgctcggca aacagtgcca atgcgtctgc
     7081 cccgataccc aaaccgattt tgtccagccg tccgcgtatc cattggggca aagccgccgc
     7141 gccgaccggt ttggcttccc acacttcccc ctttgccgcc aatgcggcaa accatttgga
     7201 ctggagctgg gttttctcca gtttgggcag taaaaccagc gttaccgtat cttccggcag
     7261 gcgggcggca aaatcctgca acgcctcgcc gccggttttg ccgggcttgc cgttagggat
     7321 atgcagttcc aacagcttca aatcggcaaa cagacccgca ctgcctgcgg tttgcagcag
     7381 ctcgttccaa tcgaaagcat tgtctgccgt ataaacttcc cgattaaggt aaccttgttt
     7441 cttcgccgcc gccctcaatg cgtccaatgc ctcgatacgc aacagttctt cctcgccgtg
     7501 gatgacgtac aggggtttca aaggcgcgtc cgtatcaatg cgtccgatat gtgccgccac
     7561 gtcattccgc cttcagaaag gtcaggcggc ggacaatctg ttcggcggca tcctgccgca
     7621 tttccgccca caatgccgct tcctcttcct gtttgcccaa gatctcgttg tcggcataag
     7681 caaggacgcg gcggacggac acggtcatcg gtttgccgac cggctcgccg cgtttcaata
     7741 cctgcgcttc aaccgtcaat atcaaaagat attcgttgat gactgccgca cgggtaacgg
     7801 tgtaggtttc cttgttttgg gaaacgctgt ctatacgcag ggtcatctgc gcgccggcag
     7861 catcgtccac cctgcccgaa gcctgataca gcgctgtttc caaaggaaac cgcaatgcct
     7921 gtccgccttc gatgtgccag ctccggtagg tcagcggcgg agaaatgccg tctgcacctt
     7981 tcaggtggaa accgcacgcg cccagcatca gcactgcggc agtaagaaac agtttgttca
     8041 tggcttgcct ttctcaaaag cacaaagtcg gtagattata tcagcggcgt gttcagacgg
     8101 cattgccgtc cagccaaatc aggctcgcca tacgccctgt cgctccgtcg cggcggtagg
     8161 aaaagaaagt atcccgttcc aaaaccgtac aatgcgtgcc gccgtatacg ccgcccacgc
     8221 cttcgcgctt cagaatcagg cgggcgagcg cgtaaaggtc ggcaaggaat ttgccgccgc
     8281 ctatgccttc aaatgcggcg gcggcttcgg gcatgggcgt gcagaacgca tcaaacacat
     8341 cctgtccgac ttcaaacgca tccgcactga tggcggggcc gagatacgcc atcatttcga
     8401 caggcggaac cttcatcgca gcaatggtgt tttgcaatac gccgcccgcc aaaccgcgcc
     8461 agcccgcgtg tgcggcggca accgccgtac ccgccctgtc gcaaaataga acgggcagac
     8521 agtctgcggt catcacggca caggcggcct tgcccgtgtc gtccacggaa gcgtcggcat
     8581 cgggcgtgcc gcctaacgct tcggcagcgt tgacgacgat ggtgctgtgg atttgattga
     8641 ggtaggcaac gggcaatccg acctgctgtt gcacgatttc gcggttgcgg cgcacggctt
     8701 cgggattgtc gccgacgtgc gtaccgaggt tcaaactctg atacgcacct tggctcacgc
     8761 cgccgttgcg cgtggtaatc agggttttca cattggcggg cgcgggccaa tcggcggtca
     8821 ggaagttttt gcctttcggg gcaagattta gggtttctgt gatggttttc attggatatt
     8881 ggaataggtg tgtggcggaa agggttgatt aactgccttc ggggcaaatg gatgtttgtt
     8941 ttttcgtcat tcccgcacag gcgggaattc ggagattcgg gattgttttc aaacgttcgg
     9001 gttcggattc ttgccggtgc aggaatgatg cccttatata tagtggatta aatttaaatc
     9061 aggacaaggc gacgaagccg cagacagtac aaatagtacg gcaaggcgag gcaacgccgt
     9121 actggtttaa atttaatcca ctatactttc tccgagcttt atatgtttca acagaggacg
     9181 gcacatcaag caccgcactg cgtgttgccc gaacgagact accctccgat tagattctat
     9241 cgctataaac agatgggttt caaccgaaaa ggaatgggga taaagtccat ttccgacacc
     9301 tctcgggcga tgccgtctga aaaccaatct ccactttcag acggcattgt ttaggcacgg
     9361 tcattttgca aattaaggtt aaggcgtgta tgcttgcctt cccttaaaaa caaacacgca
     9421 acgcattatt atgaatatcc tcagtaaaat cagcagcttt atcggaaaaa cattttccct
     9481 ctgggccgcg ctctttgccg ccgccgcttt tttcgcgccc gacaccttca aatgggcggg
     9541 gccttatatt ccttggctgt tgggcattat tatgttcggt atgggtttga cgctcaaacc
     9601 ttccgacttc gatattttgt tcaaacatcc caaagccgtc atcatcggcg taatcgcaca
     9661 attcgccatt atgccggcaa ccgcctggct gctgtccaaa ctgttgaacc tgcctgccga
     9721 aatcgcggtc ggcgtgattt tggtcggctg ctgcccgggc ggtacggctt ccaatgtgat
     9781 ggcctatctg gcgcgcggca atgtggcttt gtcggttgcc gttacgtctg tttccaccct
     9841 gatttcccca ttgctgactc ccgccatctt cctgatgctt gccggtgaaa tgctggaaat
     9901 ccaagcggcc ggtatgttga tgtccatcgt caaaatggtt ttgctcccca ttgttttggg
     9961 tttgattgtc cataaggttt tgggcagtaa aaccgaaaag ctgaccgatg cgctgccgct
    10021 ggtttccgtt gccgccatcg tgctgattat cggcgcggtt gttggggcaa gcaaaggcaa
    10081 gattatggaa agcggcctgc tgatttttgc ggttgtcgta ctccacaacg gcatcggcta
    10141 cctgctcggc ttctttgccg ccaaatggac cggcctgcct tatgatgcac aaaaaacgct
    10201 ggccatcgaa gtcggtatgc aaaactcggg cctggccgcc gcgcttgccg ccgcacactt
    10261 tgccgctgcg ccggtcgttg ccgttccggg cgcattgttc agcgtgtggc acaatatctc
    10321 cggctcgctg ctggcaactt attgggcggc caaagccggt aaacataaaa aaccctaaac
    10381 gcctttcatc ataatgccgt ctgaacgggg caaacgaaac cggcggtttc cgcccgaccc
    10441 cttcagacgg cctttgtttg aaatcggcag ggacgattgt ccggctgctc cgattcggct
    10501 gcctgcgttc gccccgcctt tcaagccgcc tcactccctt acataaacca cttcgacatc
    10561 gtagtcgtct tcgttccaat cgtcatcgtc gtccgcgccg aatttgtcct gccattcctc
    10621 ttcattgctc aaagacgaat ccaaaccggc ttcaagacgg aggacagaca acaaatgata
    10681 tatgtcgtcc ggaatcggtg cttcaaacga aacggtttcg ccggttttcg gatgagtgaa
    10741 acttaagcgg taggcgtgca atgcctgacg cgcacccaaa cttttaacgg cttctttcac
    10801 cgtgtcgccg cacggatggc gcgggttgcc gtaaaccggg tcggcggcaa gcggatggtt
    10861 ggcctcacgc atatggacgc ggatttggtg cgtcctgcct gtttcgagcg agcattcgat
    10921 atagctgtgc gccagatagc gttccaacac tttgacgtgg gtaacggctg gtttgccgcc
    10981 gaatttgacg actgccattt tcaggcggtt gtgcggatcg cgtccgattt gggtttcgat
    11041 tttaccgtca aagggaacga tgccgttggc gacggcgcgg taaatgcgtt tgaccgtgcg
    11101 ctcttgaagc tgttgcacga gggaattttg cgccggcagg gttttggcaa ccaccatcag
    11161 cccgctggtt tctttgtcca aacggtgtac gatgcctgca cgaggtactt ggctcaattc
    11221 gggacagtgc gccaagaggc cgttgagcag cgttcccgtc cagttgccgg ccgccggatg
    11281 caccaccagt ccggccggtt tgttgacgac gatgacggta tcgtcttcgt aaacaatatc
    11341 caaagccata ggctctggaa caaacgccag attttcctca ctcggacgga cggttacaca
    11401 aatttgctcg ccgcctatca ttttgtcttt gggttgcgaa ggtttatcgt ttacaataac
    11461 cgcgccttct ttaatccatg atgtcaggcg gctgcgcgag tagtcgggca gaagtttcgc
    11521 caacaccgca tccaaccgcc cgccggcaag ctcgagcgga acggtcagac cgatacggtt
    11581 ttcagttccg gaagctgacg caaagtctga atcgtcgcta taatcggatt cattatcaaa
    11641 ggaagtattc tgcatgaaaa aaattctttt aacggtttca ttaggtttgg cactgagtgc
    11701 ctgtgccact caaggtacgg tcgataaaga tgcccagatt acccaagatt ggagtgtgga
    11761 gaagctctat gccgaagccc aggacgaatt gaacagcagc aattatacgc gggctgtcaa
    11821 gttatacgaa atcttggaat cgcgcttccc caccagccgc catgcccggc agtcgcaact
    11881 ggataccgca tacgcctatt ataaagacga tgaaaaagac aaagctctgg cggcaatcga
    11941 acgcttccgc cgccttcacc cgcaacaccc gaatatggat tacgcgctgt atctgcgcgg
    12001 cttggtgctg ttcaacgaag accagtcctt cttgaacaaa ctggcctcgc aagactggtc
    12061 cgaccgcgac ccgaaagcca accgcgaagc gtaccaggcg tttgcggaac tcgtccaacg
    12121 cttccccaac agcaaatacg ccgccgatgc gaccgcacgc atggtcaaac tggtcgatgc
    12181 actgggcggc aatgaaatgt cggtggcgcg ctactacatg aaacgcggtg catacatcgc
    12241 cgccgccaac cgcgcccaaa aaatcatcgg cagctaccag aatacgcgtt atgtcgaaga
    12301 atcgctcgcc atattggaac ttgcctacca aaaactcggc aaaccacagc ttgccgccga
    12361 tacgcgccgc gtgttggaaa ccaacttccc gaaaagcccg tttttgacgc acgcttggca
    12421 gcccgacgat atgccctggt ggcgttactg gcattaagcc catcttcgta atgccgtctg
    12481 aagccgtttc agacggcatt ttatgtttca aacctcattc aaacggtttt ttctgccaat
    12541 agaatttata ccctctcaaa cgttgagcct tcacgccgcc gcgccccaag ccgaattgca
    12601 gcgcgcctga caaatcggta cgcagcagtt tgatgccgtg tgcgcggaca cggttctgca
    12661 ccgcttcggt cggatgtttg taggcgttgg cataaccgct tgaagcaaca gcatattcgg
    12721 gcgagacggc attgaggaac acgcccgacg aggacgtatt gctgccgtga tgtcccaaca
    12781 ccaaaacctg gctgtacagg ttgcctccat acttgccgac caggctttcc tcgcccttcg
    12841 tatccaaatc gcccgttacc agcagtgccg caccgcccgc cacaacacgc aaaacacaac
    12901 ttttcccatt atcatcgatg tttttgcgtt cagacggcat caaaaactca aaatccacgc
    12961 cgtcccattg ccaacgctgt tccgcacaat gccgcgcccc ctcataaaat tccggctgcc
    13021 cggcataaat cccgccgttc ggtatcttgc cgactgcctg aaaaccgccg tcgtggtcgc
    13081 tgtcgtgatg cgacagaacc agcttgtcca aacggcggac acccgccgca ttcaaactcg
    13141 gcacaatccc catctgcgcc gccgctgccg ttccggtgtc aaacaaaaga tgatgatttg
    13201 ccgtctgaac cgacaccgac aaaccctgcc ccgcatccca aaccgtaacc gcaacctcat
    13261 tttccggcac gccgggtgaa cggtaaaaca caaaccctgc caacagcaac accgcccacg
    13321 gacgcaaacc caagccgcgc ggcagcagca acagcaaagc ggcacacacc gccaacacca
    13381 acagcggcaa aggtgcggag gcaacggcaa actcgggcga cacatcggca agccacacca
    13441 aaaaccgcaa agtatattcc gccaaaaatg cccccaactg ttgcaaaggc gcaaacggca
    13501 cgaccgaacc cagcaacgcc agcggcgtca atacccaaga aaaccacgga atcgccaccg
    13561 cattgaccaa agggctgatt aaaggcagcg aagcaaacag ataaccgagc aaaaccagcg
    13621 acaacaccga agccgcccac tgcccgcgca cggcggtttg ccgtttccct tcgtgcaaac
    13681 gccccgaaca cgcccatatc agggccgcca ccaaaccgaa agacagccaa gtccccacac
    13741 ccaagaccgc caaagggtcg aacagcagca ctgccgccaa cgcctgccac cacgtcgccc
    13801 acgccgacaa tcttcccctg cgccaagccc acgcaaacgc cgccaacatc aaaacgctgc
    13861 gctgcgtcgg cacggaaaaa ccggcaagca gcgcgtaaaa cagcgcgcct gcacaccccg
    13921 ccgccaaaac ccacacgcgc ggccgggcag gaaggcgcgg ggaacaggca agcagccgct
    13981 tcgccagcca cgcaaacatc accgccacca tcgtaacgtg caaacccgaa atactgacca
    14041 aatgcgtcag ccccaacggt cggaacgcct gccacaattc ggggcgcaat gccgactgtt
    14101 cgcccacgct caacgcgcgc atcagcccga tgccgtctga aagcccgccg tccgcatccg
    14161 cctgctgcca attacggctg atgcggctgc gccaaaccgc aatcccccaa ccgcttccgc
    14221 catgcagcaa aaccctgtcc gcaccgaccg tccccgcgcc gcctatcccg ttggataatg
    14281 cccacgcctc acggttcaaa ccgcgcaggt tcaattcgcc gacgacgggg tgcacacgtg
    14341 ccgttatccg ccatctgctc ccgaccgccc attcgcgccg tttgtagtcc gacagcagca
    14401 aatcaaacgt ccgaccaccg ctgtccacag cctttgccgc aaactgcacg cgccgcccgt
    14461 ccgacctcgg catatccgcc acttccaccg tcaacggcac acctgaaacg gcttctgccc
    14521 accattggga agacaatgcc gcctccgtcc tgaatacgcc gtaagccgcg cccgccaaca
    14581 cgcacagcat cagaccggca aacgcaaaac gccttgcaaa cacagcaaaa accgcaaaag
    14641 ccgccagcca aaacagccaa tgcggcacga ccggcagcgc gaacgaagcc gccgcaccga
    14701 caacccaaca cggcaaaaac caacgacgga acacgtctcc ccaccccaga tatttcaaaa
    14761 ataaacggat aataaagcaa atccgaaaaa ccgtcataac aagatatgaa aatgatatag
    14821 tggtttaaat ttaaatcagg acaaggcgac gaagccgcag acagtacaag cagtacgaaa
    14881 ccgattcact cggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
    14941 acgctgtact ggtttttgtt aaaccactat aagaatgcaa acgaaaagcc tcagtattat
    15001 ccgagacaac ggcaaacttt ccatcgtcat tcccgcacaa gcgggaatcc aaaaacaaaa
    15061 agccgcagaa atttaacgga agaaccaaac cccgaaaacc ggctccccgc cttcgcttga
    15121 ataacagaac aaaggaatca gtattatccg agacaaccgc aaactttcca tcgtcattcc
    15181 cg
//
LOCUS       DAWHXS010000047        14948 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWHXS010000047 DAWHXS010000000
VERSION     DAWHXS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.047"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(35..661)
                     /locus_tag="WIE22_001121"
     CDS             complement(35..661)
                     /locus_tag="WIE22_001121"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ4127214.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(768..1586)
                     /locus_tag="WIE22_001122"
     CDS             complement(768..1586)
                     /locus_tag="WIE22_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4127215.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGTVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(1845..2285)
                     /locus_tag="WIE22_001123"
     CDS             complement(1845..2285)
                     /locus_tag="WIE22_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ4127216.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSETSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(2348..3754)
                     /locus_tag="WIE22_001124"
     CDS             complement(2348..3754)
                     /locus_tag="WIE22_001124"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ4127217.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            3925..5040
                     /gene="lptF"
                     /locus_tag="WIE22_001125"
     CDS             3925..5040
                     /gene="lptF"
                     /locus_tag="WIE22_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ4127218.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRNSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLSLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLVYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVDKSLTLKGGK"
     gene            5037..6107
                     /gene="lptG"
                     /locus_tag="WIE22_001126"
     CDS             5037..6107
                     /gene="lptG"
                     /locus_tag="WIE22_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ4127219.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRFFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            6418..9003
                     /gene="acnB"
                     /locus_tag="WIE22_001127"
     CDS             6418..9003
                     /gene="acnB"
                     /locus_tag="WIE22_001127"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ4127220.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASLLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            9145..10140
                     /locus_tag="WIE22_001128"
     CDS             9145..10140
                     /locus_tag="WIE22_001128"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ4127221.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDG
                     IEYRGFAQETVEELTKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTSFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(10209..10310)
                     /locus_tag="WIE22_001129"
                     /pseudo
     CDS             complement(10209..10310)
                     /locus_tag="WIE22_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10364..11377)
                     /gene="ilvC"
                     /locus_tag="WIE22_001130"
     CDS             complement(10364..11377)
                     /gene="ilvC"
                     /locus_tag="WIE22_001130"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ4127222.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(11457..11750)
                     /locus_tag="WIE22_001131"
     CDS             complement(11457..11750)
                     /locus_tag="WIE22_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ4127223.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(11817..12308)
                     /gene="ilvN"
                     /locus_tag="WIE22_001132"
     CDS             complement(11817..12308)
                     /gene="ilvN"
                     /locus_tag="WIE22_001132"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ4127224.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(12319..14046)
                     /gene="ilvB"
                     /locus_tag="WIE22_001133"
     CDS             complement(12319..14046)
                     /gene="ilvB"
                     /locus_tag="WIE22_001133"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ4127225.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDTLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            complement(14755..14839)
                     /locus_tag="WIE22_001134"
     tRNA            complement(14755..14839)
                     /locus_tag="WIE22_001134"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14803..14805),aa:Leu,seq:caa)
BASE COUNT         3532 a         4011 c         3784 g         3621 t
ORIGIN      
        1 ttttaaaagt cttcatcatt tgctcccgcc cggtttacgc cgtcaggaaa cgggcggcat
       61 cggcgttttc cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat
      121 cggcaacggc tttcggatag agtttgtgtt cgacagccaa aacccgggcg gcaatatcgt
      181 ctgccgtatc accatcaagt atcggcacaa tcccttgcga tacaatcggg ccgcaatcca
      241 gctcggccgt aacaaagtga atcgtacagc ctgcaacgcg gcaacccgct tccaaagcgc
      301 gttcgtgcgt atgaagccca gtaaatgaag gcaggatgga cggatggatg ttcatcagcc
      361 tgccttcgta atgggcgcaa aactcggggg tcagaatccg cataaaacct gccaaaacca
      421 ccaaatcggg ttgatatgcg tcgatttttt ccatcatggc cgtatcgaag gcaagtcgtg
      481 attcaaagtt tttatgattc aggctatcgg tcgggatgcc gcgttcggcc gcccattgca
      541 aaccggcagc cgtttcgctg ttgctcaaca cggcggcaat gcggacgttg ggaatggcgg
      601 cattgacgat tgcctgcata ttgctgccgc gtccggaaat caggatgacg atgtttttca
      661 taatggtacg cttttgaaag ggatgccgtc tgaaccgctg tttggtggtt tcagacggca
      721 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
      781 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
      841 agtttcacat caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg
      901 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagctgt
      961 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgt gccgccgttg
     1021 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
     1081 atgtcgtctt tggtcggctg tttgccttca ccctgtttgg tgattttgta ttgcaggccg
     1141 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
     1201 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
     1261 aggaatttca tcatgacttc ctgagcctgc tcttcggtca ttttgatttc tttgccgtca
     1321 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcgcc ctgttccttc
     1381 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
     1441 ctgccgatcg aagaggtgtc gccctgcgcg gaagaagcgg cggcaggttc ggatgcagat
     1501 gcgggggcgg cttctttttt gccgcaggcg gaaagtgcca aagcggcgga aagggtcagt
     1561 gcgctgattt tgaaaatggt gttcatgatg gatcttcgct gtcgataagg tcggaaaaac
     1621 gggattatag ccgagtttga atgtttcaac acacaggatg acacataaag cgtcaatcgt
     1681 gtgttgccct gttttggaag ggattgaacc ttccaaaata agttttgatt ctaccgcccc
     1741 gagggacaga tgtccaagtg gcggggttca accgataagg aaattttaat caaatagaat
     1801 caagcctgtt taaattttgt aaatgcggca tttcagacgg cattttatgc cttgccctcc
     1861 atgccgtgat gttcgatggc aaaaccgctt cggcggtagg cggtaaagcg ttcgcgcgcg
     1921 tcggcgagat cttccaagct gttgccgacg atttccaaaa cgcgcgcggg caagaccgga
     1981 gcggtgttcc aaaaaccgtc ggacaggttc aaaacggtca tgccttcggg aattcggggc
     2041 agattgccgc cgcaggcaag caggacggat gtttcagacg gcatggcttc ttccgtttcc
     2101 caaatttcgt gcggaatgaa actctcggcc tcgtattgcc aaagcatttt gtccaattcc
     2161 tgaagctgcc cgaacgaatc ggaccacacc agtatcctgc cgccgtcccg aatcgcacgg
     2221 gcaatcaggc ggcaggtgaa aatcggaact tgggcaacgt gcgtgtaaaa ggtggctttc
     2281 ggcatattgt ttgaacattt ggcaggataa tgccgtctga aaggcttcag acggcattgt
     2341 ggcaaaatta aagattccgc agatagttca gcagcaaggg aacgggacgg ccagtcgcac
     2401 ctttttccgc accggatttc cacgccgtac ccgcgatgtc aaggtgtgcc catggatagt
     2461 cttcggtaaa gtaggatagg aatgttgcgg cggtaatcgt gcccgcgccg ggcgtgccga
     2521 tgtttggaat gtcggcaaag ttggatttga gttggtcttt gtaggtctca aagagcggca
     2581 gttgccatgc tttgtcgtcc acgttgtagg aagcggcaag caggctgtcg atcaaatcct
     2641 gattgttgcc catcacgccg ctgacatcgt gccccaaggc gacgatgcat gcaccggtca
     2701 gggtggcgac gtcgatgacg gctttgggtt tgaactgctc ggcgtaagtg agcgcgtcgc
     2761 acaaaatcag acggccttcg gcatcggtgt tcaacacttc gatggtcaga cctttcatac
     2821 ttttcacgac atcgcccggt ttgtttgccg cgccggaagg catattttca caagtggcga
     2881 cgacggcaat caggttaatc ggcagttgca gtttgacggc ggcgcagaag gtgctgatga
     2941 cggttgccgc gccgcacata tcaaacttca tttcgtccat gttcaggccg ggcttgaggg
     3001 agatgccgcc ggtgtcgaag gtaatgcctt tgccgaccaa taccacaggc gcggcttctt
     3061 tgtcggctgc accgaaatag ctcagttcga ccaaataggg ggcttctgcg ctgcctttgg
     3121 cgaccgacca aaacgaaccc atgttttctt tgatgtagtc tttttcgatg attttggcgt
     3181 gcgcgcccag tttttcggct tcggctttgg cggtgcgcgc taaaaattcg ggcgtgcatt
     3241 cgttgggcgc ggcgttgccc aagtcgcggc agaggctttg tccgtaaact tgcgcttcgg
     3301 cgacgcgcaa ggcttcttta acggcggctt cgtgtgcgct gtggaacacg gcggtttcaa
     3361 attttgcggg cttggcttct tttttgtagc ggtcgaaacg gtaggcggcg ttgccgaacg
     3421 caatcgcaaa cgcttcggct acggcggcgg cttgcgcttc atcgaaggca tggatgtcca
     3481 cattgaccgt ttcctgattt tgcgcccatt tggcggcttc ggcggcggcc ttgttcaatg
     3541 cggcgcggcc ggtgcttttc agacagcata cggcaacagc ctgcaaaccg ttgcctgtcg
     3601 ggattttggt gtcggcaaaa ttttgacctt cttcaagcga agacaaaagg gcaagggcgg
     3661 tcggatggtt taattgcgcc tcttcggtgc agacaaataa ctgtgcgcct gcctgctgtt
     3721 cctgcaagat ttcggttttt gtgctaaatt ccacgtttat tctcctgatt gagacggttg
     3781 tcggtagttt tcggacggcc tttcgctcaa aagaccgtct gaagacggct ggcacgattg
     3841 taccccattt gaagcaccgt ctgaaacctt gcgcggacaa tccgcctgcg ccgaaccgct
     3901 taccgccccc ctgaccgcga ttctatgatt tatcaaagaa acctcatcaa agaactctct
     3961 tttaccgccg tcggcatttt cgtcgtcctc ttggcggtat tggtctccac gcaggcaatc
     4021 aacctgctcg gccgtgccgc cgacgggcgt gtcgccatcg atgccgtgtt ggcattggtc
     4081 ggcttctggg tcatcggtat gacgccgctt ttgctggtgt tgaccgcatt tatcagtacg
     4141 ttgaccgtgt tgacccgcta ctggcgcaac agcgaaatgt cggtctggct atcctgcgga
     4201 ttggcattga aacaatggat acgcccggtg atgcagtttg ccgtgccgtt tgccgttttg
     4261 gttgccgtca tgcagctttg ggtgataccg tgggcagagc tacgcagccg cgaatacgct
     4321 gaaatcctga agcagaagca ggaattgtct ttggtggagg caggcgagtt caacagtttg
     4381 ggcaagcgca acggcagggt ttattttgtc gaaaccttcg ataccgaatc cggcatcatg
     4441 aaaaacctgt tcctgcgcga acaggacaaa aacggcggcg acaacatcat cttcgccaaa
     4501 gaaggtaact tctcgctgaa cgacaacaaa cgcacgctcg aattgcgcca cggctaccgt
     4561 tacagcggca cgcccggacg cgccgactac aatcaggttt ccttccaaaa actcagcctg
     4621 attatcagca ccacgcccaa actcatcgac cctgtttccc accgccgcac cattccaacc
     4681 gcccaactga ttggcagcag caatccccaa caccaggcgg aattgatgtg gcgtatttcc
     4741 ttgaccgtca gcgttttgtt gttgtgtctg cttgccgtgc cgctttccta tttcaatccg
     4801 cgcagcggcc acacctacaa catcctgatt gccatcggtt tgtttttggt ttaccaaaac
     4861 gggctgaccc tgctttttga agccgtggaa gacggcaaaa tccatttttg gctcggactg
     4921 ctgcctatgc acatcatcat gttcgtcatc gcaatcgtac ttctgcgcgt ccgcagcatg
     4981 cccagccagc ccttctggca ggcggttgac aaaagtctga cattgaaagg cggaaaatga
     5041 acctgatttc acgttacatc atccgtcaaa tggcggttat ggcggtttac gcgctccttg
     5101 ccttcctcgc tttgtacagc ttttttgaaa tcctgtacga aaccggcaac ctcggcaaag
     5161 gcagttacgg catatgggaa atgctgggct acaccgccct caaaatgccc gcccgcgcct
     5221 acgaactgat gcccctcgcc gtccttatcg gcggactggt ctctctcagc cagcttgccg
     5281 ccggcagcga actgaccgtc atcaaagcca gcggcatgag caccaaaaag ctgctgttga
     5341 ttctgtcgca gttcggtttt atttttgcta ttgccaccgt cgcgctcggc gaatgggttg
     5401 cgcccacact gagccaaaaa gccgaaaaca tcaaagccgc ggccatcaac ggcaaaatca
     5461 gcaccggcaa taccggcctt tggctcaaag aaaaaaacag cattatcaat gtgcgcgaaa
     5521 tgttgcccga ccataccctg ctgggcatta aaatctgggc ccgcaacgat aaaaacgaac
     5581 tggcagaggc agtggaagcc gattccgccg ttttgaacag cgacggcagt tggcagttga
     5641 aaaacatccg ccgcagcacg cttggcgaag acaaagtcga ggtctctatt gcggctgaag
     5701 aaaactggcc gatttccgtc aaacgcaacc tgatggacgt attgctcgtc aaacccgacc
     5761 aaatgtccgt cggcgaactg accacctaca tccgccacct ccaaaacaac agccaaaaca
     5821 cccgaatcta cgccatcgca tggtggcgca aattggttta ccccgccgca gcctgggtga
     5881 tggcgctcgt cgcctttgcc tttaccccgc aaaccacccg ccacggcaat atgggcttaa
     5941 aactcttcgg cggcatctgt ctcggattgc tgttccacct tgccggacgg ttcttcgggt
     6001 ttaccagcca actctacggc atcccgccct tcctcgccgg cgcactgcct accatagcct
     6061 tcgccttgct cgccgtttgg ctgatacgca aacaggaaaa acgctaaacc aatgccgtct
     6121 gacccccttc ggacggcatt ggtttttatt atcatattcc tgtagacaga tagccgttcc
     6181 ctattatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatttgt
     6241 actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatac attacctgtc
     6301 ataacagttc catttttgtt aaaactagtc tatgatagcg gtacaaatat tgtttacaat
     6361 atttaacgca aatcatttgc aacccgacaa aagaaaaaca gaaaaaggaa caaagagatg
     6421 ttagaagcct atcgtaaagc cgccgccgag cgcgccgccc tcggcattcc cgccctccct
     6481 ttgaacgcgc agcaaaccgc cgatttggtt gagctgctga aaagcccgcc cgcaggcgaa
     6541 ggcgagttct tggtcgaact gcttgcccac cgtgttccgc ccggtgtgga cgatgccgcc
     6601 aaagtcaaag cctcactcct ggctgccgtt gccgaaggca gcgcgtccag cccgctgatc
     6661 tcccccgaat atgcgaccga actcttaggt acgatgctcg gcggttacaa tatccacgcc
     6721 ttaatcgaac tcttggacga cgacaaactc gcgcccattg ccgccaaagg cttgaaacat
     6781 acgcttctga tgttcgattc cttccacgac gttcaggaaa aagccgaaaa aggcaacaaa
     6841 tacgcgcaag aagttttgca atcttgggca gatgccgaat ggttcgcctc acgcgccaaa
     6901 gttcccgaaa aaatcaccgt taccgttttc aaagttgacg gcgaaaccaa tacagacgac
     6961 ctctcccccg cgcccgacgc gtggagtcgt cccgatattc cgctgcacgc gctggccatg
     7021 ctgaaaaacc cgcgcgacgg catcacaccc gacaaaccgg gcgaagtcgg tccgattaaa
     7081 ttgttggaag aactcaaagc caaaggccat ccggttgctt acgtcggcga cgtggtcggt
     7141 actggttctt cacgcaaatc cgcgaccaac tccgtcattt ggcataccgg cgaagacatt
     7201 ccgttcgtgc cgaacaaacg cttcggcggc gtatgtttgg gcggcaaaat cgcgccgatt
     7261 ttcttcaata cccaagaaga ttccggcgcg ctgccgattg aagtcgatgt atctgctcta
     7321 aaaatgggtg atgtcgtcga tatcctgcct tatgaaggca aaatcgtgaa aaacggcgag
     7381 actgttgccg agtttgaatt gaaatcacaa gtattgctgg acgaagtgca agccggcggc
     7441 cgtatcaacc tgattatcgg ccgaggtctg accgccaaag cgcgcgaagc cctgaaactg
     7501 cctgcctcta ctgcattccg cctgccgcaa gcgcctgccg aaagcaaagc cggtttcacc
     7561 ttggcgcaaa aaatggtcgg ccgcgcctgc ggtctgcccg aaggacaagg cgtgcgtccg
     7621 ggtacttact gcgaaccgcg tatgacgacg gtcggctcgc aagacacgac cggcccgatg
     7681 acccgcgacg agttgaaaga cttggcttgt ttgggcttct ccgccgatat ggtgatgcag
     7741 tctttctgcc acaccgccgc ctatccgaaa cctgtcgatg taaaaaccca taaagaactg
     7801 cccgccttta tttccacccg tggcggcgtg tcactgcgtc cgggcgacgg cgtcatccac
     7861 tcgtggctca accgcctgct gctgcccgat accgtcggca ccggcggcga cagccacacc
     7921 cgtttcccca tcggtatttc cttccccgcc ggctccggct tggttgcctt tgccgccgca
     7981 acgggcgtaa tgccgctcga tatgcccgag tctgtattgg tacgcttcag cggcaaactg
     8041 caaccgggtg taaccctgcg cgatttggtg aacgccattc cgctgtacgc aatcaaacaa
     8101 ggtttgctga ccgttgccaa agccggtaag aaaaacatct tctccggccg catcctcgaa
     8161 atcgaaggcc tgcctgattt gaaagtggaa caagcctttg aattgaccga cgcatccgcc
     8221 gaacgctccg ccgccggctg taccgtgaag ctcaataaag agccgattat cgagtacatg
     8281 aaatccaacg tcgtgttgat gaaaaacatg attgccaacg gctatcaaga tccgcgcact
     8341 ttggaacgcc gcatcaaagc tatggaaaaa tggctggcaa atcccgagtt gctcgaagcg
     8401 gataaagatg ccgaatacgc cgccgtgatt gaaatcaaca tggacgacat caaagagccg
     8461 attatcgcct gcccgaacga cccggacgac gtgtgcttca tgtccgaacg ctccggcacc
     8521 aaaatcgacg aagtattcat cggttcgtgt atgaccaaca tcggccactt ccgcgccgcc
     8581 tccaaacttt tggaaggcaa ggcagacacc cccgtccgcc tgtggattgc gccgccgacc
     8641 aaaatggacg cgaaacaatt gtccgacgaa ggacactacg gcgtactcgg acgtgccggc
     8701 gcgcgtatgg aaatgccggg ttgctcctta tgtatgggta atcaggcgca agtacgcgaa
     8761 ggtgcgaccg ttatgtccac ctccacccgc aacttcccga accgtttggg taaaaacacc
     8821 tttgtttacc tcggttcggc ggaattggca gcgatttgct ccaaactggg taaaatcccg
     8881 accgttgaag aatatcaagc caatatcggc atcatcaacg aacagggcga taaaatctac
     8941 cgctatatga acttcaacga aatcgacagc tacaacgaag tagccgagac cgtgaacgtt
     9001 taatccccgt catccgtatg aagtaaggga ttgaccgcaa tgccgtctga acaaccttca
     9061 gacggcattg caacattccg ctaacccttc tttccgcaaa cgctgcaaat acggcgttca
     9121 cgcccccaca taaaggaaac aacagtgaac ctgaaaaacc gccattttct gaaactttta
     9181 gacttcacgc cagaagaaat caccgcctac ctcgaccttg ccgccgaatt gaaagccgcc
     9241 aaaaaagcag ggcgcgagat tcagcggatg aaagggaaaa acatcgccct gatttttgaa
     9301 aaaacctcta ctcggacgcg ctgcgcgttt gaagtcgccg cgcgcgatca aggcgcggga
     9361 gtgacttatt tagagccgtc cgccagccaa atcgggcata aggaaagcat caaagacacc
     9421 gcccgcgtgt tgggcaggat gtacgacggc atcgaatatc gcggcttcgc tcaggaaact
     9481 gtcgaagaat tgacgaaata cgcgggcgta cccgtgttca acgggctgac caacgagttc
     9541 catcccacac aaatgcttgc cgacgcactg actatgcgcg aacacagcgg caaacctttg
     9601 aaccaaacct cgtttgccta cgtcggcgac gcgcgttaca acatgggcaa ttccctgctg
     9661 attttagggg caaaattggg gatggacgtg cgtatcggcg caccgcaaag cctgtggccg
     9721 tctgaaggca ttatcgccgc cgcacacgcc gccgccaaag aaaccggtgc aaaaattacc
     9781 ctgaccgaaa acgcgcatga agccgtgaag aatgttgatt ttattcatac cgatgtgtgg
     9841 gtcagcatgg gcgagccgaa agaagtctgg caggaacgca tcgatttgct gaaagattac
     9901 cgcgttacgc ccgaactgat ggcggcatcg ggcaatccac aggtcaaatt catgcactgc
     9961 ctgcccgcct tccacaaccg tgaaaccaaa gtcggcgaat ggatttatga aaccttcggc
    10021 ttaaacggcg tggaagtaac ggaagaagta tttgaaagcc cggccggcat cgtgttcgac
    10081 caagcagaaa accggatgca cacgattaaa gcggtgatgg ttgcggcttt gggcgactga
    10141 cagaactgtg cctgtttaaa ttcatccgca acacagatac cgtctgaaca cgatgttcag
    10201 acggtatcca tatatagtgg attaaattta aaccagtacg gcgttgcctc gccttgccgt
    10261 actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa
    10321 aaaactgcct acacgatgtg taggcagtcc cgtttgaaaa caatcagttt ttgtcttggt
    10381 caaccaattt gttggcagta atccaaggca tcatggcacg cagttgtgcg ccgacttttt
    10441 caacttggtg gtcggcattc agacggcggc gggcagtcat agacgcatag ttgacattac
    10501 cctcttggat aaacattttt gcgtattcgc cggtttggat gcgtttcagg gcattgcgca
    10561 tggcttcttt gctggaagca ttgaccactt cagggccggt aacgtattcg ccgtactccg
    10621 cattgttgga aatggagtag ttcatattgg caataccgcc ttcgaaaatc aggtcaacaa
    10681 tcagtttcat ttcgtgcaga cattcgaagt aagccatttc aggcgcgtaa ccggcttcgg
    10741 tcagggtttc aaaacctgct ttaatcaact cgaccacgcc gccgcacaat acggcttgtt
    10801 cgccgaacag atcggtttcg gtttcttcgc ggaaagtggt ttcaatcaca ccgcctttgg
    10861 tgccgccgtt ggcagccgca taagacaggg cgatgtcttt ggctttgccg gaattgtctt
    10921 ggtaaacggc aatcagagaa ggcacgccgc cgccgcgttt gtattcactg cgtacggtat
    10981 gacccggacc tttgggagca accataatca cgtccaagtc ggcacgcgga acaatttggt
    11041 tgtagtgcac gttgaagccg tgtgcaaatg ccagcgttgc gccttctttc aaattggctg
    11101 taacttcggc gtgatagacg gcaggcatgg tttcgtcagg cagcagcagc ataacgacat
    11161 cggcttcttt ggtcgcttca gcaacggttt tgacgacatg accggctgct tcggcttttt
    11221 tccaagaaga accttggcgc agaccaatca ccacgtttac acccgaatct ttcaggttgg
    11281 cggcatgggc atgaccttgc gaaccgtaac cgatgatggc aacggttttg cctttgatca
    11341 gggacagatc ggcatcttta tcgtaataga cttgcatttg atttccttta aggtaaatgg
    11401 ttgtcgaagc cttaaaatgt tgagcggctt cggacgggtt aaacagagtg tgccgcttaa
    11461 gcggcaactt cattcatcaa tacgatttcc aacgcttcgg ttttgccgtc gacggactgg
    11521 acgaaggctt ggaaatgcgc gctggcgtta tgttcgtcaa tagctgcttg ggatttccaa
    11581 ttttccacga aaacaaaacg gttcggtttg ccgatttcct gatggagatc gtagctgatg
    11641 ttgccctctt ctgcacggct ggctttgacc agttctttaa actgtgctgc cagtgtttct
    11701 gtgtattccg gtttgacggt aaccagtgcg acaattttaa tgttcgacat aaatctctcc
    11761 tgccgttcgt ttttcagacg acattcaaat accgtgccgt ctgaaaggtt acggcgttaa
    11821 attttcaaaa tacgctcacc gcgaccgatg ccggccgcgc ctgtgcgtac ggtttccaaa
    11881 atttgggcgc gtccgaccgt ttccaaaaag gaatccagct tgtctgtcga gccggtaatt
    11941 tcaatcgtat agctgcggtc ggttacgtcg atgatgctgc cccggtagat ttcggtcaag
    12001 cgtaaaaatt cgtcgcggtc tttgccggcg gcacggactt ttaccaacat cagttcgcgt
    12061 tcgacaaaac ggctttcgtt caaatcgacc actttaatca cttcaatcaa tttattgagt
    12121 tgcttggtga tttgttcgat gacctgctcg tcgccgtggg taacgatggt catacgggac
    12181 agggttttgt cttcggtcgg cgcaaccgcc aaagaatcga tattgtaatc gcgtgcagag
    12241 aacaaaccga ccacgcggct catcgcacct gattcgtttt caatcagaac agataagata
    12301 tgtcgcattt gcctctcctt acgccttttc gtccgcacgc atatgcggcg gaagtaccat
    12361 ttcgtccaaa cctttgccgt taccgaccat gggcatcaca ttctgtttct ggtcggtcag
    12421 gaagtcgata aacaccagcc tgtctttttg gttcaatgct tccaacaacg caccttccac
    12481 atcagacttc ttgtccacgc ggatgccgat atgaccgtat gcctcggcaa gtttgacgaa
    12541 atcgggcaaa gaatcgaaat aagtttccga ctctcggccg ccgtaatata tttcctgcca
    12601 ctggcgtacc ataccgagat aaccgttgtt cagcgtaatg acgttaaccg gaatccgata
    12661 ttggaaacag gtggacaact cttggatgtt catctgaatc gagccgtcgc cggtaataca
    12721 gaatacgtct tgatccgggg cggcaagttt tgcaccaatc gcataaggca gacccacgcc
    12781 catcgtaccc aaaccgccgg aattgagcca ttggcgcgga cgttcgaagg gataatattg
    12841 agccgcaaac atttgatgct gccctacatc cgatgtgatg attgccgaat tgccggtaat
    12901 ctcggcaagc ttctgaatca catattgggg cttgataatt tcgctgccgt tgtcaaacca
    12961 caagcaatct cgggaacgcc attcctctat ggttttccac catttgccca aagtatcttc
    13021 agacggcacg gactcttgtt tttgccacag cgcaaccatc tcggacaaaa cgtttttcac
    13081 gtcgccgaca atcggaatgt ccgccttcac gcgtttggcg atgctggaag gatcgacatc
    13141 gatatggata accttcttcg ccttctcgaa aaatttggac ggtacggaaa ccacacggtc
    13201 gtcaaaacgc gcacctacgg caagaacgac atccgcattc tgcatggcaa ggtttgcctc
    13261 gtaagtaccg tgcataccga gcataccgag gaattggcgg tcgctggaag gataagcgcc
    13321 caagcccatc agcgtacccg tgcacggagc acccgtcatt cggacaaatc gggtcagctc
    13381 ttcagaagca ttacccaaca ccacgccgcc gccaaaatag acgactggac gtttggcaga
    13441 tgccaacatc tgcacggcct ttttaatctg accgatatgc ccttgaacaa ccggttgata
    13501 cgaacggata aaaatgtctt cctgaggata gctgaatttc gccatcgcct gcgtaacatc
    13561 tttcgggaca tcaaccacca caggccccgg tcggccgctt gcggcaattt ggaacgcctt
    13621 tttaatggtt tccgccaact cattgatatc cgtaaccaag aaattgtgtt tgacgcacgg
    13681 acgggtaata cccaccgtat caacttcttg gaacgcatcc gtaccaatca gggaattgcc
    13741 tacctgcccg ctgatgacca ccatcggaat cgaatccgta taggcagtag caataccggt
    13801 cagtgcattg gtaacgcccg ggccggatgt aaccaatgcc acgcccacct taccgctgac
    13861 gcgcgcatac gcatctgccg cgtgtactgc cgcctgctcg tgacgggtca gaatgtgctt
    13921 gaatttattg agttggaaaa gggcatcgta gatttcgata accgcaccgc cgggataacc
    13981 gaaaacgtac tcgacacctt cggctttgag actctgcact atgatttgcg cgcctgatag
    14041 ctgcataacg acctctttta tacggtttca aaccaatagg gacaaaccgc tttgccacag
    14101 cacctgtaat gcaattccac caagcagcga tttagggtac gcgcattggg ggaacacggc
    14161 aacagacgga ttatccaatc aattggaaag gaacacagag tttgtgaaaa agagtggaaa
    14221 cgataacgca aaccgacagt tcaatcaaga aaaatctttc atcttttaat attttttgaa
    14281 agcagagaaa ttattgattg attttaaaag aataaaatca ggagtacctt ttttgaaaga
    14341 tggaaattgt tgacagtttg tgtaggaggg gcagatgtga aaaacccttc ttcgatatca
    14401 agaattgtaa aatttacagg gtttcatccc aataaagact cgggatattg attgaacttg
    14461 atttcatttt tgatatatca aaaatattcc caaccatact tcctgaaaat ggctcattgc
    14521 accggactgt attggacggc attgacagaa ccaagagggc taacaacgac ttaatatatt
    14581 gattgtatag tggattaaca aaaatcagga caaggcgacg aagctgcaga cagtacaaat
    14641 agtacggcaa ggagaggcaa cgccgtactg gtttaaattt aatccactat atttagtctt
    14701 atctatttca ttaaacagca atagacaaaa aaataaccgc tccaaaagcg gttgtggtgc
    14761 ccagggtcgg actcgaaccg acacaccttg cggcggggga ttttgagtcc cctgcgtcta
    14821 ccaatttcgc cacctgggct ggtgaagaag tcgtcattat aatggctttt gaaattctgt
    14881 aaaccttttt ttgaaattat tttatctgtt tttattttat ttttgatttt aaatagaatt
    14941 tttattat
//
LOCUS       DAWHXS010000048        14871 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWHXS010000048 DAWHXS010000000
VERSION     DAWHXS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.048"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            52..1005
                     /locus_tag="WIE22_001135"
     CDS             52..1005
                     /locus_tag="WIE22_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ4127226.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WIE22_001136"
     CDS             complement(1116..1439)
                     /locus_tag="WIE22_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127227.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WIE22_001137"
     CDS             complement(1612..2973)
                     /locus_tag="WIE22_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ4127228.1"
                     /translation="MLKKWLNKMLPSSRSSKEAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKEAGFQAYVVGGAIRDLLLGIEPKDFDVATDAMPEQVHKLFRRSRIVGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3328..5028)
                     /gene="recJ"
                     /locus_tag="WIE22_001138"
     CDS             complement(3328..5028)
                     /gene="recJ"
                     /locus_tag="WIE22_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ4127229.1"
                     /translation="MSAKIQTRSVNTDVFNHLLTAGADPLIAQLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVGRKEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5097..5816)
                     /locus_tag="WIE22_001139"
     CDS             complement(5097..5816)
                     /locus_tag="WIE22_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127230.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPPFLILDA
                     AGAVLALPAAALGAVVDAARK"
     gene            complement(5863..6375)
                     /locus_tag="WIE22_001140"
     CDS             complement(5863..6375)
                     /locus_tag="WIE22_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ4127231.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MESQGGTGEA"
     gene            6529..7860
                     /gene="pmbA"
                     /locus_tag="WIE22_001141"
     CDS             6529..7860
                     /gene="pmbA"
                     /locus_tag="WIE22_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ4127232.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            7872..8063
                     /locus_tag="WIE22_001142"
     CDS             7872..8063
                     /locus_tag="WIE22_001142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127233.1"
                     /translation="MPSEDEGSTFCPVPTRLPFYAAPPFADNIAHFFISTDFPLSVKI
                     LSHLSTTGYTQVQDKACGD"
     gene            complement(8157..8360)
                     /locus_tag="WIE22_001143"
     CDS             complement(8157..8360)
                     /locus_tag="WIE22_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ4127234.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8603..8905
                     /gene="clpS"
                     /locus_tag="WIE22_001144"
     CDS             8603..8905
                     /gene="clpS"
                     /locus_tag="WIE22_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ4127235.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8907..11198
                     /gene="clpA"
                     /locus_tag="WIE22_001145"
     CDS             8907..11198
                     /gene="clpA"
                     /locus_tag="WIE22_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ4127236.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPSKNPLSAYTVNLNTEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTPALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKPETETV"
     gene            complement(11245..14343)
                     /locus_tag="WIE22_001146"
                     /pseudo
     CDS             complement(11245..14343)
                     /locus_tag="WIE22_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14374..14483)
                     /locus_tag="WIE22_001147"
                     /pseudo
     CDS             complement(14374..14483)
                     /locus_tag="WIE22_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(14528..>14871)
                     /locus_tag="WIE22_001148"
     CDS             complement(14528..>14871)
                     /locus_tag="WIE22_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ4127237.1"
                     /translation="NYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLINTS
                     SLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDILTHSISEGLPHEIALRRKQYEYY
                     REQLLAFPKAT"
BASE COUNT         3439 a         4171 c         3721 g         3540 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc gccaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tccggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcctt
     2581 cataatcctg ccgtgcgcgt tctgatgaac tttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga cgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg catggcatcg gttgcgacat cgaaatcctt
     2761 gggttcgatg ccgagcagca ggtccctgat cgcgccgccg accacataag cctgaaaccc
     2821 tgcctctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgaccgtttt actttccgct tctttactgc tccgactgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacgggca accgtgttca gacggcatcg ggcgcaggtt tgcaagatgc cgtaaccata
     3061 aacaaaaggc gtagaaacta cgccgtccga atcacgccgc cctcgcggcg gcaacgcgtc
     3121 attataacag attgccctcc aaaaaaatca gaccctgctt ttgtggcaga gtctgaaatt
     3181 tgatgcacct tatatattgc gtccctaaga agggatgatt aacaaaaatt aacgcccttt
     3241 actttctttt ccgattggga acattcccgt cgtacaaaca ggcaaatgaa ggaacagaaa
     3301 tcacattcaa acggcgttcc gccgcctcta cgccgcttcc cagtagtcga tataaagctg
     3361 caattcgagg ttgttgcgcc attcgttggc aacggggcgg tacaccgtgc ggatgtattc
     3421 gggaatgtct tcgctgcacc gccagaacat tgcttcaaat tcgcagccgt ctttttgcag
     3481 ccacgctttt ttgtgtttgc cttccgcgcc caaaggctgc tggcggacga cgtggaactc
     3541 gtcggtaaaa ctcggcggcg cgaagccctg cccccaaacg tgtttagcaa ggttttgcgc
     3601 ctgttccagc gtaatgtcgc gtgcaggcag gctgccgtcg gtgatgaagg tttgcgacaa
     3661 atcgtcttcg cacaccattt cgcgcacggc ttcttcaaag gccgtctgaa acgcgggaat
     3721 gttgtgttcg agtatgctca agcccgccgc catcgcgtgt ccgccgaatt tcaaaatcaa
     3781 atcgggatgg cgtttggaca ccaagtccaa agcatcgcgc aagtgaagat tgggaatgga
     3841 acgccccgaa ccgcgcactt caccgttgtc ggcaggcgca aacacgatgg tcggacgata
     3901 aaaacggtct ttgaggcggc tggcaacgat gccgaccacg ccttgatgga agtcgtcgcg
     3961 atacgccacc aaagtcatct gacctgaagg cagggtttca ggaaaagcat tcagcgcgtc
     4021 ttgcagcata gactgctcga tttcgcgacg ttcgatattg aggttgttta actgcgccgc
     4081 cagttcctgc gcctcggtat catctcgcgc caacaggcag gcaataccga ccgacatatc
     4141 gtccagccgt ccggcggcgt tgatgcgcgg gcccaacgca aaacccatat caaacggctg
     4201 cgccttgcgc caatcgcgcc gcgccacttc aaacaaggcg cggataccgg ggcgcatttt
     4261 gccggagcgc atccgtttca aaccttgcga cacgaggatg cggttgttgt ggtcgagagg
     4321 gacgacatcg gcaaccgtgc cgagtgcgac caaatccaaa agttcgccca gattcggctc
     4381 tttgatgccg tctgaaaaat aattgcggcg gcgcaattcg gcacgcaaag ccatcaatac
     4441 ataaaaaatc acgcccacgc ccgccaagct tttgcttgga aaaccgcagc ctttttggtt
     4501 cggattgacg atgatgcagt cgggcacggt ctcggcaggc agatggtggt cggtaacgat
     4561 gacatccaaa cccagagcct gcgcgcgcgc cacgcctgcg atgctggcga taccgttatc
     4621 aaccgtaatc agcaaatcca caccttgctc tgccgcgatt tcagccagct cgggcgttaa
     4681 gccgtagccg tgctcaaagc ggttgggcac aaggaaatcc actttcgccc ccatcgccgc
     4741 caaaccgctc ataccgacgg aacacgccgt cgcaccgtcg gcatcatagt cggcaacaat
     4801 caggattttt tccttgcgcc caaccgcatc cgccaaacgg cgggcggcgg cttcgcaatt
     4861 cgtcaacgat tgataaggca ggagggaagc gagtttgtcg tccaattcgg caggactttg
     4921 cacaccgcgc gaagcacaaa gctgggcgat taaaggatcg gcaccggcgg tgagcaaatg
     4981 attaaaaacg tcggtattga cggatcgggt ttggattttg gctgacatga cgaggttttt
     5041 tcaagatatg atgaggctga aatgccgtct gaaagctttt cagacggcat tgctgttcat
     5101 ttgcgggcgg catccacgac cgcacccaga gccgccgcag gcaaagccag taccgcgccc
     5161 gccgcatcca gtatcaaaaa gggggggggc gtatataaga tatttgcaaa cagcttggat
     5221 ttgtcggtat gtttttcagt aaccgtgtaa taaatatcgg caggcacact ttgctcaaaa
     5281 tgataatcgg cgttcagttt ttgcggcgtg gcgtagtatt tgcctttggc ggatacgcag
     5341 cgcgtgtaaa tagtccgatt gtcaagttcg accgcttcaa acccgagctg tttcagcttg
     5401 gcgatgtcgg caggcttgtc ggtatcgtag cgcaggcaaa ggccttcggt actgaaattc
     5461 tggctgccag gcgattcgag tttgaccggc agggcttggt ggcgagcata gctcggggta
     5521 tcctcaacta tttggaaggg tttgtccagc cctgccttca aaatgcccgt cagcttcgcc
     5581 gaatcttcgg gattgacgac gaaccagtat ttcccgccca tcatcaccag actgcccttt
     5641 tccaattggg cattgtcttt ggcaaccaca ccgaaggcgc ggatttgatc cttgtcaacg
     5701 tgtttatagg cggtcgtttg gctgaacggg tcgttcatgc cccacatcat tgccgtacag
     5761 ccgttcaaca tcaaggctgc cgccaaaacg gcggcggtcg ttttacggaa caacatttta
     5821 tcttccaatc agaatgacgg cggatgccgt ctgaaaatat gcctaggctt cccctgttcc
     5881 gccttggctt tccataacgg ttttcaactc ttgaaacagg gcgcggaaat ttttcggcgg
     5941 tttgttctgt tcctgttctt ttttggtatt gcggatgagc gtcctcagct tgcccgcatc
     6001 ggcgtttgga aaatcggaca taaactgcgt cagcgcgccg tcgtccgcca gcagctccat
     6061 ccgcgtccgc tccacacgtt gcaaaaaggc gttgtgcgcc gcatcgtcgc cgcgcagctt
     6121 ggcaagaaat gcctcgatgg gcgcgggatc ggtatcgcgc atcagtctgc cgataaattg
     6181 cgcctgccgc ttgagcgcgc cgttggatgt gattttttta taggtaacga tggcttcgta
     6241 caaatcttcg tccaagccga tttttttcaa cgtgtcgctt gagagcttgg tcagctccat
     6301 acccaaatcc tgcaaaccgt tcatctgctt tttcatttgg gttttgctga cccattcgtc
     6361 ttcttgttca aacatcttca ttctcaaatt ttatccgacg ggcgcatttt aacagaatga
     6421 ttccgcgttc agacggcatt ttgcaccgtc cgccctgcaa acggacgctt ttgcccctgc
     6481 cgcccgaaac ggttaaaatg ccgcaacgct ccaccaagaa agatttctat gctgttcaac
     6541 cacaccgctt ccgaacttct cgacctctgc cgccgcacgc tcgacttggc aaaagcagcg
     6601 ggcgcgaccg ccgccgaagc cgatttcagc gaatccatcg gacaaagcgt cagcgtgcgg
     6661 ctgggcgaaa tcgaacaaat cgagttccag caggacaagt cgctggacat taccgtttac
     6721 gtcggcaaac gcaaaggccg cgctggcacc gccgatttct ccgaacaagc cctgcgcgac
     6781 accgtccgcg ccgccgccga catcgcccgc cacaccgccg aagacggttg cgccggactt
     6841 gccgatgcct gtctgatggc aaaacacatc ggcgaccccg acctttacca cgaatgggat
     6901 ttggatacgg aagccgccgt cggcttggca aaacaatgcg aacaagccgc cctgaacgag
     6961 gatgagcgca tcgaaaactc cgaaggcgcg gcggtgcaaa ccggccatta ccaatacgtt
     7021 tacggcaaca cccacggttt tgccgcacac cggcaaggca cgcaccacag catttcctgc
     7081 agcgtcgttg ccgccgacga aaacggcatg cagcgcgact actggtacga ttccgcctgc
     7141 cgccatccgg atatggacag ccccgaaacc atcggtcaaa ccgccgcccg ccgcactttg
     7201 cgccgactcg gcagccgcag catcccgaca ggcagctacc ccgtcctctt cgataccacc
     7261 gtttcgggcg gtcttatcgg acacctcgtc ggcgcactct ccggcggtgc gctctaccgc
     7321 caaagcagtt tcctgatcga cagcatcggc aaaaaagtcc tgcccgattt cctcaacctg
     7381 cgcgaagaac cgcacatccc ccgctctttc cgcagcagct attttgatgc ggaaggcgtt
     7441 gccaccgcac cgcgcttcgt gattcaaaac ggcattgtcg aaggctattt cctcagcagt
     7501 tacagcgcgc gcaaactcgg tatgcagacc acgggcaacg ccggcggcgc gcacaacctg
     7561 tatttgaacc atacgcacga aacgcaatcc gacctgctga aagaaatggg gacgggatta
     7621 ttggttaccg agctcatggg acagggcgcg aacaccatta ccggtgacta ctcgcgcggc
     7681 gcggcgggtt tttgggtgga aaacggcgtg attgcctacc ccgtccacga gattaccgta
     7741 gccggacgct tgcaggatat gtaccgcgac attgtcggcg tggcggatga cgctttgcgg
     7801 cgttcgtcca acaaaatcgg ctcgattctg attgcgggga tgacggttgc cggaagctga
     7861 acgccctgcc gatgccgtct gaagacgaag gcagcacgtt ttgccccgtg cctacccgtt
     7921 tgccgtttta tgccgcaccg ccttttgccg acaacattgc acattttttc atcagcactg
     7981 attttccctt atccgtcaaa atactaagcc acttgtccac aacagggtac acacaagttc
     8041 aggataaggc gtgcggggat tagggttttt ccgccgccgg caaaacggca gacaaaaacg
     8101 gcgggacatt gcctgtcccg ccgttttcaa atatacattt gaccgtacat caggaattaa
     8161 gcagcctgaa tgttggcggc ctgtttgcct ttagggccgg tggttacgtc gaaagagacg
     8221 cgttggcctt ctttcagggt tttgaaacct tccatattga ttgctgagaa gtgagcgaac
     8281 aaatcttcgc cgccttcatc aggcgtgatg aaaccaaaac ctttagcgtc gttaaaccat
     8341 tttacgatac cggttgccat tagaaacttc ctatactcaa aaattaacaa aatcagcaaa
     8401 acaaggcata cggcaaaccg agccgtctgg cgttccttcc agcttgatat actcggcgga
     8461 tgcccaatcc tgcttaaccg tctttttaca tttgttaaga caaaacgtca agctatgttt
     8521 tcaaaaaagt gggaaaatag gcaaaagtac cgaaaataca acaaaaatgc cgtctgaacg
     8581 cggctttcaa atgggacaca ctatgaccgc gcaacaccaa tccgacacgc tcttgcaccg
     8641 tctgaacacc ctgccgccga aacgttacgg cgttttccta ttgaacgacg attacaccac
     8701 gatggaattt gtcgtcgaaa tcctgaccga aatctttatg ctcggacaag aacaggcggt
     8761 agcggtaatg ctcttggttc atcacgaagg caaaggcctg tgcggcactt acacgcgcga
     8821 tattgcccaa accaaacaac aacaagtcat gcagcgggca aaagccgaag ggcatccgct
     8881 gcaatgtatt gtcgaggaga tttaatatgc ttgcacccga attggaacag attttgcagc
     8941 agctttaccg cgaggcgcgt aaggctcatt atgaatttat cagcctcgag catctgcttt
     9001 tggtactcat cgaagaagat gcctccgtgc cgaacgtctt aaaactatgc ggcgcggatt
     9061 tgaaagtggt gtccgaacag ctcgccgcca gcgttgccga aaacaccccc ctgattcccg
     9121 aacacctttt agacacggtc gaaacccggc ccacgctcgg cttccaacgc gtgatgcaac
     9181 gggcgatggt gcatacccag tctgccggaa aagccgcagt cgaaccgttg gacgttttgg
     9241 tcgcgctgat gagcgaaacc gacagccaca ccgtctattt cctcaagctg caatcggtta
     9301 cgcgttttga agttttgcgc tgtattgccc atggctcgcc cgatgaagat gaagacgatg
     9361 gcaactattc ttcagacggc atggacgacg ataatgagaa ccgcaccaaa ccgagcaaaa
     9421 accctttatc ggcgtacacc gtcaacctca acaccgaagt caaagccggc cgtatcgacc
     9481 ctttgattgg tcgcaaacac gaaatggaac ggctggtgca aatcctatgc cgccgccgca
     9541 aaaacaatcc gcttttggtc ggcgaagcag gcgtgggcaa aaccgcgctg gcggaaggtt
     9601 tggcacatca aatcgtcaac ggcggcattc cagacgcgct taaagatgcc gaagtgtacg
     9661 cgctggatat gggctcgctg ttggcgggca cgaaataccg tggcgacttt gaagcgcggg
     9721 tcaaatccgt cttgaaacag ctcgaaaaaa tcccgcacgc cattttgttt atcgacgaaa
     9781 tccacaccat catcggcgca ggcagcacca gcggcggcac catggacgcg tccaacctgc
     9841 tcaaacccgc gctggcaaaa ggttcgctgc gctgcatcgg cgcgaccact tacgacgaat
     9901 accgcaccat tttcgacaaa gaccatgcct taagccgccg cttccaaaaa atcgacgtgg
     9961 tcgaacccac cgtttccgaa accgtacaaa tcctgcgcgg cttgaagccg atgtttgaag
    10021 ccttccacca agtccgctat acgcaaggcg cactcgaagc cgccgccgaa ctctccgcac
    10081 gctacatcaa cgagcgtttc ctgcccgaca aagccatcga cgtgatggac gaagcaggcg
    10141 cggcgcaacg gattctgccc aaatccaaac agaaaaaagt catcggcaaa gcgcaaatcg
    10201 aaaccgtcat cgccaaagtc gcgcggattc cagaaaaaac cgtgtcgcac gacgacaaac
    10261 aggtgctgca attcctcggc cgcgatttga aaaacatggt ttacggtcag gaaaatgcca
    10321 tcgacgcgct ggttgccgcc gtcaaaatgt cgcgttcggg cttgggactg cccgacaaac
    10381 cgataggcag cttcctcttc tccggcccga ccggcgtggg caaaaccgaa gccgccaaac
    10441 agcttgccta ctcgatgggc gtaccgctgc aacgctttga tatgtccgaa tacatggaac
    10501 gccatgccgt atcgcgcctc atcggcgcgc caccgggcta cgtcggcttt gaacaaggcg
    10561 gccttttgac cgaagccgtc aacaaacagc cgcattgcgt attgctcttg gacgaaatcg
    10621 aaaaagccca ccccgacatt ttcaacgtcc tcctgcaagt catggacgca ggcaaactga
    10681 ccgacaacaa cggcaagagt gccgatttcc gcaacgtcat tctgattatg accaccaacg
    10741 caggcgcgga aagcctcagc cgccccagcc tcggctttac cgccaagcgc gaacgcggcg
    10801 acgaaatgca ggcaatcaac aagctcttca cgcccgagtt ccgcaaccgc ttggacgcga
    10861 ttatcccgtt tgcacccttg tccgaaccca tcatcgtcaa agtcgtggac aaattcctgc
    10921 tccagctcga acaccagctc ctcgacaaaa aagtcgaagc cgaattcacg ccggcattgc
    10981 acaaatatct ggcggaaaaa ggttttgacc cgcaaatggg cgcgcgcccg atgcaccgcc
    11041 tgattcagga aaaaatccgc aaaccgctcg ccgacgaact cctgttcggc aaactatccg
    11101 acggcggctt cgtgcggata gactgggatg cggcaaaaga agaagccgtg ttgaagttta
    11161 agaaaagcaa ggttaaacct gaaacagaaa ctgtttaacc gattgaaaaa taaaatgccg
    11221 tctgaaattt cagacggcat ttttttaaat atctgtccct attccggcaa acttttccac
    11281 aaacgccgca atcttttgaa aaacactttg tttcttcgtc agatattgcg ggttgagcgg
    11341 gctcattttc ggcagggttt cggtcagttc cgtgccgttt tcgctggcat agccgcgttt
    11401 gagcgagccg gtcaaataac gtttggcggc ggtttcgttc aggtcttcgg cggcaatcag
    11461 ctctgctgct tcgcgcctca tcacctcttg agcgtaggtg taaaaggttt ccagtatggc
    11521 gggaacgtcg gggactttgt ccaaatccgt atcgttgatg aaatccacaa tcaggctctc
    11581 tttggcgcgg tggccgatgc tggcgcggat gatgcggcgg atttcttcca ccagctccgc
    11641 tttgcctttg atttttttgt ggtgctcgaa aaccagttgc aagatgtaat ccagattgat
    11701 ttcctgcgat ttgagcaaat ccacctcaaa aaccacatcg tcccaatcga tttttgattg
    11761 ttctttctgt tcgcctgctt tttggcggcg cagccagtcg cggatgtcgt tataggcaga
    11821 acggtagtcc tgcactgccc tttcagacgg catcggcact tgccgcattt cctgcacgtc
    11881 ttcatcgctc aggtagtatt tttcttgaaa cgccttcatc gcatcttcgt ccgccgcgtc
    11941 cacactctgc aactcgcgca gcgcggcaaa ttcatcgtag ttctgcaata cgttttccgc
    12001 ccgcaggtat tcgccgaaga gtttggcaaa atcttttttg tctttttccg tttcgatttt
    12061 gtcgggatcg gggaaacgct cgcgcaattc ttttgccaca tccaaataac cgcgccgcgc
    12121 ttcgccggtc tggctgtcga tatagccgtt catgtattct tcgtagcttt tttccagcac
    12181 cacgtttttg gtgtttttgt cgccgaacag ggtaatcgca tcaatggtcg cctgctccaa
    12241 atcgcggaag cagacaatat tgccgaaggt tttggtggca tcgtaaatgc ggttggtgcg
    12301 agaaaacgcc tgcatcaggc cgtgatagcg caggttttta tcgacaaaca gcgtgttcag
    12361 cgtcggcgcg tcaaaacccg tcaaaaacat gccgaccaca atcagcaaat ctatttcctg
    12421 atttttcacc cgttttgcca aatcgcggta gtagttctga aaggctttgc tgtccgtgcc
    12481 gaaattggtt ttgaaacagg cgttgtaatc gttgatggca gcctgcaaaa attctttcgc
    12541 gctgctgtcc atcgcttccg gttcaaaggt ttcatcgaca atttcaccga cggcgttctg
    12601 ctcttcgttg gcagcaaagg aaaaaatagt ggccactttc aacgggtgca ggctgcctgc
    12661 ctgttgcgtt ttgaacgctt cgtaatagca cttcgccgca tccacgctgc tgacggcaaa
    12721 catcgcgtta aagcctttgc cgcccgcatt cagccggtgc gttttctgcc tgaactggtt
    12781 caggatatat tgcgtgattt cgcggatgcg ttcggggtgc agcagggctt ggcggttttc
    12841 ggcggcactc agtttcttct cgtcctgttc cgcttccacg gctttgaact gcgggcgcac
    12901 atcgttgtaa tccactttga atttcaatac tttttcatcg cggatggcat cggtaatcac
    12961 ataagaatgc agctcccgcc cgaacacgcc cgccgtggtt tccgcgccca aagcgttttc
    13021 gggaaaaatc ggcgtgccgg taaaaccgaa ctggcagaat tttttaaatt tctttttcag
    13081 gtttttttgc gcttcgccga attgcgagcg gtggcattcg tcgaaaataa agacaacccg
    13141 ctgatggtaa accggcagat tatcttcact tttcatcagg ttgttcagct tttggatggt
    13201 ggtaacgatg attttgttgt cgtctttttc caaattgcgt ttcaagcccg ccgtgctttc
    13261 cgaaccgttc acgctgtcgg tcgaaaaacg ttggtattcc ttcatcgtct gatagtccaa
    13321 atccttcctg tccaccacga agaaaacctt gtcgataaat gccgattccg tcgccagacg
    13381 cgccgcctta aagctggtca gcgttttgcc gctgcccgtg gtgtgccaaa catagccgcc
    13441 gctctccggc ttgctccaat tcttcgcctg cgccgagctg ttgattttcc acagaatgcg
    13501 ttcggcggcg gcaatctggt acggccgcat aatcagcagc gtatcattcg catcgaacac
    13561 gctgtaatgc agcaaaacgc tcagtaatac gcttttttgc aggaacgtgg cggtaaagtc
    13621 tttcaaatcc ttaatcggat gattgtccga ccgcgcccaa ttcatcgtga aatcgaagct
    13681 gtttttgtcg cgcttggtgg tgttggcgaa atagcgcgtg tccgtgccgt tggaaatgac
    13741 gaagatttgc aggaatttga acaacgaatt gtcgctgttg aagctctctt tgctgtaacg
    13801 gtgcacctga ttgaatgctt cacgcaccgc cacaccgcgc tttttcaatt caatctgcac
    13861 cagcggcagg ccgtttacca acacggtaac gtcataacgg tttgcatgcg tgcccgtctg
    13921 ctcaaactag ttgataagct gcacatggtt gcgggcaagg tttttcttgt ccagcagata
    13981 aatgtttttc agacggccgt catcaaaagc gaaatcgtaa atatggtcgt cgtggatttt
    14041 gcgggttttg tcggtaatgt tttcagacgg cctgtccaga tattccgtta aaaaccgcgc
    14101 ccattcgccg tctgaaaacg ccacatcgtt cagccgctgc aactgcgcgc gcaggttttc
    14161 cagcagcctg ctttggctgt tcaagtcttt gcggtattcg taaccctgat tctgcaaatc
    14221 ggcgattaac tccgcttcca actggttttc cgattggtag ctgcccgact gttcgatttt
    14281 ttcatattgg tcgagcacga tgaaattcgg cgtttcagcg atgggtttag tttcgaggtt
    14341 catatgggtc gtcctttgcg gtgtcgggtt ttatagtgaa ttaaatttaa accggtactg
    14401 cgttggctcg ccttgccgta ctatttgtac tgtctgcggc tcgccgcctt gtcctgattt
    14461 aaatttaatt cactataaac ggtttgatgt tcggattggg ctgcctgaaa atgtgcaggc
    14521 tgctttttca ggtagcctta gggaaggcaa gcagctgttc gcggtaatat tcgtattgtt
    14581 tgcggcgcag ggcgatttcg tgcggcaggc cttcgctgat agagtgggtc aggatatcga
    14641 atttgtctag gatggcggca attttttcct gttcggggag tggtgggatg gggattttta
    14701 atttctttaa ttctttcatg ctaataatag gtaaacttga agtattaata agttttttgc
    14761 tctgctccgc aataatgaaa aaataataga aagcgaaatt tatatctaaa gcatccttaa
    14821 attcatcttt aattgtaaaa ttggtcagtt gttggttact aatataatta a
//
LOCUS       DAWHXS010000049        14815 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWHXS010000049 DAWHXS010000000
VERSION     DAWHXS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.049"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(122..1447)
                     /locus_tag="WIE22_001149"
     CDS             complement(122..1447)
                     /locus_tag="WIE22_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ4127238.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1659..2498
                     /locus_tag="WIE22_001150"
     CDS             1659..2498
                     /locus_tag="WIE22_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ4127239.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2495..3661
                     /locus_tag="WIE22_001151"
     CDS             2495..3661
                     /locus_tag="WIE22_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ4127240.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRIHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3925..5190
                     /gene="efeB"
                     /locus_tag="WIE22_001152"
     CDS             3925..5190
                     /gene="efeB"
                     /locus_tag="WIE22_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ4127241.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5262..5591)
                     /locus_tag="WIE22_001153"
     CDS             complement(5262..5591)
                     /locus_tag="WIE22_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ4127242.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5668..7038)
                     /gene="glmU"
                     /locus_tag="WIE22_001154"
     CDS             complement(5668..7038)
                     /gene="glmU"
                     /locus_tag="WIE22_001154"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ4127243.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLAADVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            complement(7093..7365)
                     /locus_tag="WIE22_001155"
     CDS             complement(7093..7365)
                     /locus_tag="WIE22_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ4127244.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPIYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7341..8072)
                     /locus_tag="WIE22_001156"
     CDS             complement(7341..8072)
                     /locus_tag="WIE22_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ4127245.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMTRRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPRKYERMLCVKPSLS"
     gene            8131..9132
                     /locus_tag="WIE22_001157"
     CDS             8131..9132
                     /locus_tag="WIE22_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4127246.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9226..10074
                     /locus_tag="WIE22_001158"
     CDS             9226..10074
                     /locus_tag="WIE22_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ4127247.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10100..10585
                     /locus_tag="WIE22_001159"
     CDS             10100..10585
                     /locus_tag="WIE22_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ4127248.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10687..12255)
                     /gene="msrAB"
                     /locus_tag="WIE22_001160"
     CDS             complement(10687..12255)
                     /gene="msrAB"
                     /locus_tag="WIE22_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ4127249.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12401..13666
                     /gene="ftsY"
                     /locus_tag="WIE22_001161"
     CDS             12401..13666
                     /gene="ftsY"
                     /locus_tag="WIE22_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ4127250.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKEAVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGESIDDLRPFDARAFVDALLD"
BASE COUNT         3248 a         3761 c         4412 g         3394 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgcctag ggatgggctt cagacggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggcagaag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta accccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc gcccaaggta atgtagtgcc
      361 ggtcgactgc gccggcgtac tcgcccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgctgct tccggtaagc tctcggaaaa agatatagct ggggttttgc cccaaaactt
      481 cggcgaggcg ttgcgggttt tgctgcatat aggctttgat gccctgcatc gaggtctgcc
      541 cgagcttgag gtagcctttg tccgccatat agcgtccgat ggaaacgtag ggatgttcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacgg ggtcttcggc gtaaccgagt atcggggctt
      721 tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gccgttgtgc gcgcggtgat ggggaatcgg gagaggtcgg
      841 cggtgtgcgt gccgccggca ttgtcgattg tgccgctgtt tttccccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gctcgtaata gccggtaacc gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcacacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggcacaa
     1261 cggtataaac ggccccgccg ccggcaaccg tcgttccggc gggggcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgcacag ggcggcgcgg aacaggtatt
     1441 ttttcataat ggacaatgtt gtcagcagta ataaaaaaag atggtttcgg gcggcgttgc
     1501 ggcagccgtg gagaggggat tttaacacag ggcgcagctg cagcctgcgg aactttccgc
     1561 cgcgcggtac tgcagataaa aataacttgc atttgtattt acaagcaatg aaaatattcc
     1621 gataatatat tattcatcat ccttgttcgt tcgcgtttat gctggtcgct tttttaatta
     1681 tgttgcgcga gggtatcgaa gccgcgctca tcgtcggcat cgttgccggt tttctgaaac
     1741 agtccggaca ttccaaactg atgcctaagg tctggttcgg ggtcgtcctt gcttctttga
     1801 tgtgtttggg catcggatac ggcatccatt cggcaacggg cgagattccc cagaagcagc
     1861 aggagttcgt cgtcggcatt atcggtttgg ttgctgtcgc gatgctgact tatatgattt
     1921 tatggatgaa aaaggcggcg cgttcgatga agcagcagct tcaggattct gtacaggcgg
     1981 ctttgaaccg tggcagcggt caaggatggg ccttggtcgg tatggcgttt cttgccgtgg
     2041 cgcgtgaagg tctggagagt gtttttttcc tgcttgccgt attcaaacag agcccgactt
     2101 ggcagatgcc ggccggcgcg gtagcggggg ttttggctgc cgccgtgatt ggcgcgttga
     2161 tttatcaggg tgggatgcgc ctgaatctgg cgaagttttt ccgttggacg ggggcgtttc
     2221 tgattgtcgt tgccgccggc ctgcttgccg gttcgctgcg cgcgctgcat gaggcaggta
     2281 tttggaacgc gcttcaggac attgtgttcg actcatcaaa atatttgcac gaagacagcc
     2341 cgttgggcgt gctgctcggc ggatttttcg gctataccga ccatccgacg cagggcgaag
     2401 ccttggtttg gctgctgtac cttattcccg tcatgacttg gtttttgtgc ggcagcaggc
     2461 cgtctgaaac tttaacccgt aaagaggagc tgaaatgaga aaattcaatt tgaccgcatt
     2521 gtccgtgatg cttgccttag gtttgaccgc gtgccagccg ccggaggcgg agaaagctgc
     2581 gccggcagcg tccggtgagg cgcaaaccgc caacgagggc ggttcggtca gtatcgccgt
     2641 caacgacaat gcctgcgaac cgatggaact gaccgtgccg agcggacagg ttgtgttcaa
     2701 tattaaaaac aacagcggcc gcaagctcga atgggaaatc ctgaaaggcg tgatggtggt
     2761 ggacgagcgc gaaaacatcg cccccggact ttccgataaa atgaccgtca ccctgttgcc
     2821 gggcgaatac gaaatgactt gcggtctttt gaccaatccg cgcggcaagc tggtggtaac
     2881 cgacagcggc tttaaagaca ccgccaacga agcggatttg gaaaaactgt cccaaccgct
     2941 cgccgactat aaagcctacg ttcaaggcga ggttaaagag ctggtggcga aaaccaaaac
     3001 ctttaccgaa gccgtcaaag caggcgacat tgaaaaggcg aaatccctgt ttgccgacac
     3061 ccgcatccat tacgaacgca tcgaaccgat tgccgagctt ttcagcgaac tcgaccccgt
     3121 catcgatgcg cgtgaagacg acttcaaaga cggcgcgaaa gatgccggat ttaccggctt
     3181 ccatcgtatc gaatacgccc tttgggtgga aaaagacgtg tccggcgtga aggaaattgc
     3241 agcgaaactg atgaccgatg tcgaagccct gcaaaaagaa atcgacgcat tggcgtttcc
     3301 tccgggcaag gtggtcggcg gcgcgtccga actgattgaa gaagtggcgg gcagtaaaat
     3361 cagcggcgaa gaagaccggt acagccacac cgatttgagc gatttccaag ccaatgtgga
     3421 cggttcgaaa aaaatcgtcg atttgttccg tccgctgatc gaggccaaaa acaaagcctt
     3481 gttggaaaaa accgatacca acttcaaaca ggtcaacgaa attctggcga aataccggac
     3541 taaagacggt tttgaaacct acgacaagct gggcgaagcc gaccgcaaag cgttacaggc
     3601 ctctattaac gcgcttgccg aagaccttgc ccaacttcgc ggcatactcg gcttgaaata
     3661 agccgcaagc gttcagacgg tatttggcgg cagataccgt ctgaagtttt atagtggatt
     3721 aacaaaaacc agtacggcat tgcctcgcct tgccttgccg tactatttat actgtctgcg
     3781 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ccgccatata ttgcagggcg
     3841 ggatttcaac ctgccgctat cggttaatgg aaaaacggcg tgcagggata cccatcctgc
     3901 tgcacggata ttgaaggaaa caccatgagc aaaaaccaac ccgcacaacc gaccaggcgc
     3961 actcttttta aaaccgcgat cgcagccgga gcagtcggcg caatcggagg ttatctcggc
     4021 ggcaaaaaac ggggcgaaac cgccgaacgc accgccgaaa gtcaacactc gccccaagcc
     4081 tatccctgct acggcgaaca tcaggcaggc atcgttacgc cgcagcaggc gttttcgatt
     4141 atgtgcgcct tcgacgtaac cgcgcaaagt gccaagcagc tggaaaacct gttccgcacg
     4201 ctgaccgccc gcatcgagtt tctcacccaa ggcggcgaat accaagacgg cgacgacaaa
     4261 cttccgccag ccggcagcgg cattttgggc aaagccttca accccgacgg gttgaccgtt
     4321 accgtggggg tgggcagcag cctgtttgac ggccggttcg gactcaaaga caaaaaaccg
     4381 attcatttgc aggaaatgcg cgacttctcc aacgataagc tgcaaaaaag ctggtgcgac
     4441 ggcgatttga gcctgcaaat ctgtgccttc acccccgaaa cctgccaagc cgccctgcgc
     4501 gacatcatca aacacaccgt ccaaaccgcc gttatccgct ggagtatcga cgggtggcag
     4561 cctaaatccg aacccggcgc gatggcggcg cgcaacctgt tgggcttccg cgacggcacg
     4621 ggcaacccca aagtttccga ccccaaaacc gccgacgagg ttttgtggac gggtgtggcc
     4681 gccaacagcc tcgacgaacc ggagtgggcg aaaaacggca gctatcaggc agtccgcctt
     4741 atccgccact ttgtcgagtt ttgggacagg acgccgcttc aagagcaaac cgacattttc
     4801 gggcggcgca aatacagcgg tgcgccgatg gacggcaaaa aagaagccga ccaaccggat
     4861 tttgccaaag accccgaggg gaataccacg cccaaagaca gccatatacg cctggcgaat
     4921 ccgcgcgatc ccgaattcct caaaaaacac cgcctcttcc gccgcgccta cagctattcg
     4981 cgcggactcg cctcaagcgg acagcttgat gtcgggctgg tgttcgtctg ctatcaggca
     5041 aaccttgccg acggattcat cttcgtgcaa aacctcctca acggcgaacc gctggaagaa
     5101 tacatcagcc ccttcggcgg cggctatttc ttcgtcttgc ccggcgtgga aaaaggcggc
     5161 tttttggggc aagggctgct gggcgtataa atccgccata taaaaaacgc cgtccgaacc
     5221 ttgccaaacg ggttcggacg gcgtttcttg tttttgggcg gtcaggcttt tttgacgaat
     5281 tcggatttta aattcatcgc gctgccgtcg attttgcagc cgatgttgtg atcgccttct
     5341 tgcaggcgta tgcctttgac ttttgtgcct tgtttgatca ccatcgagct gccttttacc
     5401 ttgaggtctt tgatgaggat gacggtatcg ccgttttgca gcactgcgcc gttggcatcg
     5461 agcacttgag ccgcaaggtc ggcggcggat tcggtttcat tccattcgtg ggcgcattcg
     5521 gggcagatgt attgtccgcc gtcttcataa gtgtattcgg aggcgcattg tgggcatggg
     5581 ggtaatgaca tggtttgcag tccttatctg atatcggagg agtttgatgc cgtctgaaac
     5641 ctgaaaccgg cttcagacgg catagcttta ttgcttgtct ttttcaggcc gcatccagcc
     5701 ttcgatgacg gtttggcggg cgcgggcgag ggcgagtttg ttgtcttcga tattgcgggt
     5761 aatcgtgctg cctgcgcctg tggttacttt gttgccgagg gtaacggggg cgactaggac
     5821 gcagtttgaa ccgatgcgga cttcgtcgcc gataacggtt ttgtgtttgt gcacaccgtc
     5881 gtagttggca ataatcgtac ctgcgccgaa gttggttttg ctgccgactt cggcatcacc
     5941 aatgtaggtg aggtggttgg ctttggtgcc tttgccgatg gcggcgtttt tgatttcgac
     6001 gaagttgccg acgtgtacgt cggcggctag tttggcttgc ggacgcaggc gggcataggg
     6061 gccgattcgg ttgttttcgc cgacttcgca gctttcgagg tgggagaagg gggcgatttt
     6121 gctgtttgcg ccgattttgg cgtttttgat gacgcagttt gcgccgattt cgacgttgtc
     6181 gccgagttcg acttcgcctt caaagataca gttcacatca attacgacat cctgtccgtg
     6241 tttcagacgg cctcgtaaat cgaaacgtgc cggatcgcgc agggttacgc ctgctttgag
     6301 caattcttgc gcctgttcgg tttggaagat gcgttcgagt tcggcgagct ggcgtttgtt
     6361 gttcacgccg gcggcgaggt gggaggcgcg gacttggacg ggatgaactt taataccgtc
     6421 ggcaacggct ttggcaatga ggtcggttaa ataatattcg ccttgagcat tgttgctgga
     6481 gaggctgttc agccagtttt ccagtttggc gttgggcaga accaaaatgc cggtattgat
     6541 ttctttcacg gctttttggg cggcgtcggc gtctttttct tcgacaatgg cggttacgct
     6601 gccgctgccg tcgcggatga tgcgccccaa acctgacggg tcggcgggca cgtcggtcaa
     6661 cagcccgact tcgttgcctg cggcttcgag cagggtttcg agggtttcaa cgtcaattaa
     6721 aggaacgtcg ccgtacaaca ccagcgtgcg gccttcggcg gcaaggtggg gcagggcggt
     6781 tttgacggcg tggccggtac cgagctgttc ggtttgttca acccaaacga catcgcgttt
     6841 gacagcgttt aaaacttggt ctttgccgtg cccgatgacg acgcagatgt tttgcggatt
     6901 cagcgcggcg gcggtgtcga taacgcgccc gagcatgggc ttgccgccga tgcggtgcag
     6961 cactttgggc attttggaat acatgcgcgt gcctttgccg gcggcgagga taacgatgtt
     7021 taaagtgttt tgcggcatga cggtttcctg tgcaatgccg tctgaaaggg cttcagacgg
     7081 catagggtgg gtttatcggt tttgaaattt gggcttttgc cagtgttggc gatgctcttc
     7141 gtcggcgttg ttgccggttt ggttgggtaa cacggcatgg cgttcgggac ggtattggtt
     7201 gtagttcata tttttcgagt agctgccgtc ttggtaataa atgggcgtgc cggcgggata
     7261 tttttgacgg acggcggtct tgccgttgcc gtcttgataa gtttcccacg cgcagcccga
     7321 caaaagggcg gcggcagtca tcaggataag gaaggtttta cgcataacat tctttcgtat
     7381 tttcgggggg ttgtgcgggt attgtaatga ttttggcggt gttctgacaa agtttctgca
     7441 taccgagcca gttgcgccat atcgcttacg gaagcatcga taaagggcag gtcgtgcact
     7501 tttgcaccga accggacggt tttcatgccc aatgcttttg cctgatgcag gttgtccgcg
     7561 ctgtcgtcca ccataatgca gcattcgggc ggtacgtcca acaggcggca gacattgaga
     7621 tacgcttgcg gattgggttt gtacagcagc ccgaaatcat ccgtgccgaa aagcgcgtcg
     7681 aaacggtttt ccaaaccgag tgcgccgaca acggcacgga cgtaaaacga cgggccgttg
     7741 gaaaaaaccg ccttgcgccc ttttaggcgg ctcagggtgt tttgtgtttc cggcataccg
     7801 tgcagacggg tcaaaacctc gtcaatcgga tggctttcgc gcaaaaattc ggcgatgtcg
     7861 atttcgggat ggtggatttg cagtccggcg agcgttgcgc cgtagcggtg ccaatagtct
     7921 tgacgcaggt cggacgcggc ggattcggag agtttgaggc ggcgtgtcat atagcgtgtc
     7981 atagcgcggt tgatgagtgt gaagaggccg gcatcggcat tgtgcagcgt gttgtcgagg
     8041 tcgaacagcc acacggtcgg gttttcttgc attttgaacc gtgaaaattt gttagaatgt
     8101 tattttacag cgaatagagg aggactcgga atgaaacgga aaatttggct gctgccgctg
     8161 ctggcggttt cggcatacct gcaggcgcag acggaagtca ggctggcggt gcataagtcg
     8221 ttcagcctgc ccaaagggct gattgcgcgc ttcgagcggg caaacgatgc gaaggtgtcg
     8281 attattcagg cgggcggcgc gaacgaaatg ctcaacaaac tgattttgag ccgcgccaac
     8341 ccgattgccg acgcggtgta cggtttggac aacgccaata tcggcaaggc gcgggaaatg
     8401 ggcattttgg cggcggcgca acccgaatcc gcccccgtcg cggtcgggct gccttcggct
     8461 ttggcggtcg attacggcta tgtgtccatc aattacgaca aaaaatggtt tgaaggcaaa
     8521 aagctgcccc tgccgcaaac cctgcaggat ttgacccgcc ccgaatataa aaacctattg
     8581 gtcgtgccgt cccccgccac gtcgtccccg gggctgggct tcctgatggc gaacatcagc
     8641 ggtctgggcg aagaaggcgc gttcaaatgg tgggcacaga tgcggcagaa cggcgtgaag
     8701 gtcgccaaag gctggagcga ggcgtattac accgactttt cgcacaacgg cggcgcgtat
     8761 ccgctggtgg tcggttatgc cgccagcccg gcggcggaag tgtatttttc caaaggcaaa
     8821 tacagcgagc cgccgacggg caacctgttt ttaaaaggcg gcgtattccg ccaggtcgaa
     8881 ggcgcggcgg tcttgaaggg cgcgaaacag ccggaattgg cggcaaaact ggtgcaatgg
     8941 ctgcaaagtc gggaagtgca gcaggcggtt ccgtccgaaa tgtgggttta ccccgccgtc
     9001 aaaaacacgc gcctgcccga cgtgttccgc ttcgcccaag ccccgacgca caccaccgcc
     9061 cccgcgcagc gcgatattga tgcgaaccag cgcggatggg tttcccgttg gattagaacg
     9121 gttttgaaat aaaacaaaca taccgccccg cagggcttca tacggcattt ttacacctgt
     9181 gccgattacg ccgcacgggg cggatgttcg atcaagagga aaacaatgga cttcaaacaa
     9241 tttgattttt tacacctgat cagtgtttcc ggttgggagc atctggctga aaaggcgtgg
     9301 gcgttcgggc tgaaccttgc cgccgcgctg cttatttttt tggtcggaaa atgggcggcg
     9361 aaacgcattg tcgctgtgat gagggcggcg atgacgcgcg cgcaggtcga tgccacgctg
     9421 attagttttt tgtgtaatgt tgccaatatc ggcttattga ttttggtgat tattgccgca
     9481 ttgggcagat tgggcgtttc cacaacatcc gtaaccgcct taatcggcgg cgcgggtttg
     9541 gcggtggcgt tgtccttgaa agaccagctg tccaattttg ccgccggcgc gctgattatc
     9601 ctgttccgcc cgttcaaagt cggcgatttt atccgcgtcg gcggttttga aggatatgtc
     9661 cgagagatta aaatggtgca gacttctttg cggacgaccg acaacgaaga agtcgtgctg
     9721 cccaacagcg tggtgatggg caacagcatc gtcaaccgtt ccacactgcc gctgtgccgc
     9781 gcccaagtga tagtcggcgt cgattacaac tgcgatttga aagtggcgaa agaggcggtg
     9841 ttgaaagccg ccgtcgaaca ccccttgagc gttcaaaacg aagagcggca ggccgccgcc
     9901 tacatcaccg ccttgggcga caatgccatc gaaatcacat tatgggcttg ggcaaacgaa
     9961 gcagaccgct ggacgctgca atgcgacttg aacgaacaag tggtcgaaaa cctccgcaaa
    10021 gtcaatatca acatcccgtt cccgcaacgc gacatacaca tcatcaattc ttaaacgccg
    10081 tctgaaaggg gagtgggaaa tggacgcgct gcacaccatc gcccgaaacc tgacgaaaaa
    10141 acgtcaaacc gtaagctgtg ccgaatcctg tacgggcgga atgcttgccg ccgcattcac
    10201 aagcgttgca ggcagttcgc aatggttcga tcaaagtttt gtaacataca gcaacaaagc
    10261 caaagaagac cgcttgggcg tgttgcccga aaccctgctc gaacacggcg cggtcagccg
    10321 ccaaaccgtc tatgagatgg cgcgcggcgc gaaagccgtg gcgcaggcgg attacgccgt
    10381 cggtatttcc ggcatcgccg gaccgggcgg cggcagcgaa agcaaacccg tcggcacggt
    10441 ttggttcggg tttgcctttc cgggcggaag ttgcgaagca atgcgccgtt ttgacggcaa
    10501 ccgcgaatcc gtccgcgcgc aggcggtcgc cttcgcgttg gaacggttgg cggggctgat
    10561 tgaaaacggc ggcgatgccg tctaaacaaa atctccgtct ggacaaaatc cccgccggat
    10621 aaaaaatacc gtctgaaacg ttgcaggttt cagacggtat tttgtcgggt taggcggcgg
    10681 tgcggcttat ttcactttac tcttcaacgc gccgtagcct gccgcgtcca tttgttccag
    10741 cgggatgaat ttcaagctcg cgccgttgat gcagtagcgc agtccgcctt tgtcgcgcgg
    10801 gccgtcgggg aagacgtgtc ccaaatgcga atcggcggcg tggctgcgca cttcggtgcg
    10861 gcgcatgttg tagctgaaat catcgtgttc ggtaacggat tttgcatcaa tcgggcgcgt
    10921 gaagctcggc cagccgcagc cggaatcata tttgtcggcg gagctgaaca aaggttcgcc
    10981 gctgacaacg tccacataaa tgccgggttt gaacaaatgg tcgtattcgt ggctgaaggc
    11041 gtattcggtc gcgctgtttt gggtcacttg gtattgctct tcggtcaggg tgcgtttgag
    11101 ttcggcatca ctgggttttt tatacgttgc cgcatcgaag cctttgcctt gcggggcggc
    11161 tttggttttg cccggcagtg gttcgtcggc tttgcggata tcgatgtggc agtagccgtt
    11221 ggggttttta atcaggtagt cctgatggta ttcctcggca tcgtagaagt ttttcagcgg
    11281 ctcgttttca acaacgaggg gcagttggta tttttgctgc tcgcgtttga gggcggcggc
    11341 gatgacggct ttttcggcgg ggtcggtgta gtacacgccg ctgcggtatt gcgtaccggt
    11401 gtcgttgccc tgtttgttga ggctggtcgg atcaacgacg cggaagaaat attgcaggat
    11461 gtcgtcgagg ctgagtttgt cggcatcgta ggtaactttg acggtctcgg cgtgccccgt
    11521 atggcggtag gacacgtctt cgtagctcgg atttttcgtg ttgccgttgg cgtagccgga
    11581 taccgcgtca accacgccgt cgatgcgttg gaaataggct tccaagcccc agaagcagcc
    11641 gccggcgagg taaatggtgc gcgtgttcat gatttttgaa tcctttttct gagtgtcggg
    11701 tttgtagaac gaatgtttca agctgcccaa atcggcattc gggtcgcgga ttaacgccaa
    11761 cgcctgcgct tcgttgatgc tgcctttgac gatgcgctgc acgtcgccgt ctttaccgat
    11821 taacgcccac gaggggtaaa cgctgatatt caggctttgg gcgatcgtgc cgccgttgtc
    11881 ggttacgacg ggcagcttgg gataattcaa accggcatac catttttgga agtcgccgtc
    11941 ttttttctcg tgcaaaaagc ccggagacgc gacggtaatc aggttggcgg agctgaattt
    12001 tgcatcttgc gcccattttt cggtctgtcc caattcggac agacacaaag gacaccagct
    12061 cgcccaaaat ttaatcagcg tcggtttgtc ttttttcaag taaacactgg cggggcggtt
    12121 gtctgcggtt ttcaaagtgg ataaagtgtg cggcacggtc gcggctccgg catcgacgat
    12181 tttgggcgaa caagcaccca gcgcaagcag gcagccgaac ttggcgcaaa gggaaaagaa
    12241 agtacggtgt ttcattttga tgtttcctgt gtggacggtt tgtatgatta gacgtttgag
    12301 atgccgaaac cttacagctc ggattttcag acagccttgc cgcgtaaaat acgctacaat
    12361 acgccctatt tcaagtttct aaaattaaaa ggaaaattca atgttcagct tcttccgtcg
    12421 caagaaaaaa caggaaacgc cggctcccga ggaggctcaa gttcaggaaa ctgcagcaaa
    12481 agcagaatcc gaagttactc aaatagttga aaatattaaa gaagaagtcg aatctttaac
    12541 ggaaagcgtc aaagggcagg tcgaatcggc tgttgaaacc gtcagcggtg cggttgaaca
    12601 ggtaaaggaa gccgttgccg agatgctgtc tgaagcagag gaagcggcgg aaaaagcagc
    12661 ggaacaagtc gaagcggcaa aagaagccat tgccgaaacc gtcggcgagg ctgtcgggca
    12721 agttcaagaa gccgttgcaa caactgaaga acacaagctc ggttgggcgg cgcgtttgaa
    12781 acaaggtttg accaaatcgc gcgacaaaat ggcgaaatcg ctggcgggcg tgttcggcgg
    12841 cggacaaatc gacgaagatt tgtacgaaga gctggaaacc gtgctgatta ccagcgacat
    12901 gggcatggaa gccaccgaat acctgatgaa agacgtgcgc gaccgtgtca gcctcaaagg
    12961 gctgaaagac ggcaacgaat tgcgcggcgc gttgaaagaa gccttgtacg acctgattaa
    13021 gcctctggag aaacctttgg ttttgcccga aaccaaagag ccgtttgtca tcatgcttgc
    13081 cggcatcaac ggcgcgggca aaaccacgtc tatcggcaaa ctcgccaaat atttccaagc
    13141 gcagggcaaa tctgtgctgc ttgccgccgg cgatactttc cgcgccgccg cccgtgagca
    13201 gcttcaagct tggggcgaac gcaacaacgt aaccgtgatt tcgcaaacca cgggcgattc
    13261 cgccgccgtg tgcttcgatg ccgtccaagc cgccaaagcg cgcggcatcg acatcgtgct
    13321 tgccgacacc gccggccgcc tgcccacgca gcttcatttg atggaagaaa tcaaaaaagt
    13381 gaaacgcgtg ctgcaaaaag ccatgcccga cgcgccgcac gaaatcatcg tcgtgcttga
    13441 tgccaacatc gggcaaaacg ccgtcaacca agtcaaagcc tttgacgacg cattagggct
    13501 gaccggttta atcgtgacca aactcgacgg cacggcaaaa ggcggcatcc tcgccgcgct
    13561 tgcttccgac cgccccgtcc ccgtccgcta catcggcgtg ggcgaaagca tagacgacct
    13621 gcgcccgttt gacgcgcgcg cgtttgtgga cgcactgctg gattgagccg aaatgccgtc
    13681 cgaaaacagc agaccgatgc cgtcattccc gagcaggcgg gaatctagac cttgggataa
    13741 cagcaatatt caaaggttat aaaagacccg tcattcccgc gcaggcggga atctagacct
    13801 tagaacaaca gtaatattca aaggttagct gaagctttag agattctgga ttcccacttt
    13861 cgcgggaatg acgggatttg agattgcagc atttatcgga aaaaacagaa accgctccgc
    13921 cgtcattccc gcgcaggcgg gaatctagac cttagaataa cagcaatatt caaagattat
    13981 ctgaaagtcc gggattctgg attcccactt tcgtgggaat gacgggattt gagattgcgg
    14041 catttatcgg aaaaaacaga aaccgctcta ccgtcattcc cgcgcaggcg ggaatctaga
    14101 acgtaaaatc taaagaaacc gtgttgtaac ggcagaccga tgccgtcatt cccgcgcagg
    14161 cgggaatcta ggtctgtcgg cacggaaact tatcgggtaa acggtttctt gagattccgc
    14221 gtcctggatt cccactttcg tgggaatgac ggaatgtagg ttcgtgggaa tgacgggatt
    14281 tgagattgcg gcatttatcg gaaaaacaga aaccgctccg ccgtcattcc cgcgcaggcg
    14341 ggaatctaga ccttgggata acagcaatat tcaaagatta taaaagaccc gtcattcccg
    14401 cgcaggcggg aatctagaca ttcaatgcta aggcaattta tcggaaatga ctgaaactca
    14461 aaaaactaga ttcccacttt cgtgggaatg acgggatttt aggttcgtgg gaatgacgga
    14521 atgtaggttc gcgggaatga cggaatgtag gttcgcggga atgacgcggt gcaggtttcc
    14581 gtatggatgg gttcgtcatt cccgcgcagg cgggaatcca gaccttagaa caacagcaat
    14641 attcaaaggt tagctgaagc tttagagatt ctggattccc actttcgtgg gaatgacgga
    14701 atgtaggttc gcgggaatga cgcggtgcag gtttccgtgc ggatggattc gtcattcccg
    14761 cgcaggcggg aatccagacc ttagaacaac agtaatattc aaagattata aaaga
//
LOCUS       DAWHXS010000050        14525 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWHXS010000050 DAWHXS010000000
VERSION     DAWHXS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.050"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(27..103)
                     /locus_tag="WIE22_001162"
     tRNA            complement(27..103)
                     /locus_tag="WIE22_001162"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Asp,seq:gtc)
     gene            complement(136..211)
                     /locus_tag="WIE22_001163"
     tRNA            complement(136..211)
                     /locus_tag="WIE22_001163"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(176..178),aa:Val,seq:tac)
     gene            complement(281..2257)
                     /locus_tag="WIE22_001164"
     CDS             complement(281..2257)
                     /locus_tag="WIE22_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ4127251.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLKGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2406..3245
                     /locus_tag="WIE22_001165"
     CDS             2406..3245
                     /locus_tag="WIE22_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ4127252.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALAQIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3445..4476
                     /gene="gap"
                     /locus_tag="WIE22_001166"
     CDS             3445..4476
                     /gene="gap"
                     /locus_tag="WIE22_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ4127253.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4730..5455)
                     /locus_tag="WIE22_001167"
     CDS             complement(4730..5455)
                     /locus_tag="WIE22_001167"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ4127254.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGSV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5525..5959)
                     /gene="fur"
                     /locus_tag="WIE22_001168"
     CDS             complement(5525..5959)
                     /gene="fur"
                     /locus_tag="WIE22_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ4127255.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            6178..6555
                     /locus_tag="WIE22_001169"
     CDS             6178..6555
                     /locus_tag="WIE22_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ4127256.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            6565..7374
                     /gene="dapB"
                     /locus_tag="WIE22_001170"
     CDS             6565..7374
                     /gene="dapB"
                     /locus_tag="WIE22_001170"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ4127257.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7638..8048)
                     /locus_tag="WIE22_001171"
     CDS             complement(7638..8048)
                     /locus_tag="WIE22_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127258.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(8050..8295)
                     /locus_tag="WIE22_001172"
     CDS             complement(8050..8295)
                     /locus_tag="WIE22_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127259.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(8303..9475)
                     /gene="lpxB"
                     /locus_tag="WIE22_001173"
     CDS             complement(8303..9475)
                     /gene="lpxB"
                     /locus_tag="WIE22_001173"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ4127260.1"
                     /translation="MWGQTNMADKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9523..10515)
                     /locus_tag="WIE22_001174"
     CDS             complement(9523..10515)
                     /locus_tag="WIE22_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ4127261.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            11010..13766
                     /locus_tag="WIE22_001175"
     CDS             11010..13766
                     /locus_tag="WIE22_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ4127262.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRNE
                     RGQTALETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALVYDAAEKIRRAAAILPEGATPKASAQEMPSETATFTAAAEQ
                     ARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEAATVPAEEMIQVE
                     TRQG"
BASE COUNT         3655 a         4686 c         3385 g         2799 t
ORIGIN      
        1 accgacagag tcagcatttt gaaacttggc gcggcggacg gggctcgaac ccgcgacccc
       61 cggcgtgaca ggccggtact ctaaccaact gagctaccac cgcgcatcca ttgtttgcaa
      121 caatgaaaga aaacttggtg ggtgatgacg gagtcgaacc gccgacattc tgcttgtaag
      181 gcagacgctc taccaactga gctaatcacc cttgcgattt gcgaaagcgt tattaaacca
      241 aataaccgga aatggcgcaa gccttatttc ggcatttcat ttatattccc tgcaaactcc
      301 agttttcaaa agagataatt tcttcttttt tgcctgcgac caccaacacg tcaccgcctt
      361 tcaatgtaaa cgaggcatcc gggttttcaa tccggccggt gcggcggcgg acgaacaaga
      421 gtttgatgcc gtaagccgcc atcggaagcg tgccgactgt tttgccgacg gcgtatgctt
      481 ctgcagccaa aggaaaggcg tgacggacgg tttcgccgtt ttcgccgaat cctgcctcat
      541 catcactacc gacaaacagt ccctccaacg cggcataacg gctgtggcgg atatttgcca
      601 tcgtctgata gacgtgccga tacgacgcgc cgttgcctaa catcgcatag cctgccagca
      661 cgagtccggt ttctttggtg tccgacacgg cttcttccgc acctatatcg gtaaacgttt
      721 tcacataatc gtcgttggtg gcgcgcacat atacgggcat attgggatac atggacagca
      781 cattgtctaa aacgtgttgc gtttcgtgca tattgttgag cgtcagcacc accattttcg
      841 cccgtcccag accggcggct tccaatactt ccctgcgttt cgcatcgccg aacgacaccg
      901 gttcgcccgc acttctggca acctgcaccc gcgcaatgtc caagtcgagc gcgaaatacg
      961 gaatatcctc ttgggcaagg acgcgtccga ccgtctgccc gcccctgccg aagccgacaa
     1021 tcagcacatg gtcggacttg ctcatggttt ctaccagcat actgtgcaga tcgagcgact
     1081 tcatgtccca gcttgacttg accaaacgcc cgaccagcgc atcgctgccg cccaagagga
     1141 agggcgcgat aatcatcgac agcagaaccg ccgctgtcgc cgcctgttcc cattctggcg
     1201 aaaccatatc aagctgcccg gcaatggcca gcatcacgaa gccgaactcg ccgccctgcg
     1261 cgagatacaa agccgttttg aggctgtcgc cgaccgaatg tttcattttg aaggcaatgg
     1321 caaacacaac cagtgccttc aacaccagca gcattgccaa cagcatcaat acctgccgcc
     1381 aaccgccgat caatgcctga atgtccagct tcatgccgac cgtgataaag aaaaagccga
     1441 gcaaaatatc gcggaacggg cggatgtcgt cttcgacttg gaaacggtat tccgtttccg
     1501 aaagcagcat gccggcaacg aatgcgccca acgccataga caaaccttcc agctcagtca
     1561 gataagccac acccaaggtt accagcagca cattgatcat aaagagttcg gacgatttgc
     1621 gttttgccac catcctgaac catcgcgaca taattttgct gccgacgaaa aacagcagcc
     1681 ccagcgtcag cagcattttt gcaaacgcca aacccaaggc cgcccaaata tttccgtccc
     1741 ctccgcccgc cagcgcggga atcagaatca tcagcggcac gacggcgatg tcctgcatca
     1801 gcagcacgcc catcgccatc tgaccgtgcg gctgccccaa ttccgtcttt tccgacaaaa
     1861 tccggctcac aatcgccgtg gacgacatcg ccaacgcgcc cgacacggca aacgcccaat
     1921 tgaacgggac gcccgtcagc atcagtatgc ccattaccga cagcatcgta acgccgacct
     1981 gcaaaccgcc cagcccgaac accagccgcc tcattgccct cagcttgggc agcgaaaact
     2041 ccaagcctat actgaacatc aggaacacaa tcccgatttc gcccaaataa tccgttgcat
     2101 gacttttcgg aatcaggctg agcataccgg gccccgccaa aaagcccacc agcaggtagc
     2161 ccagcatgga gggaatgttg aacttgcggc acaggatcac cgtaatgacc gacaccagca
     2221 aaacaaccac aataggggca agcgaaaatt cgttcatagg tcgtccgaac aggaaaatac
     2281 agaaaaatgc cgtctgaaac ggcatacgcc gccgtattat aacaaaacac cgcgcaccat
     2341 ccgaaacggg cgcggcatac aattctgcta aaatacgccc tttcgatttt gagccgcaca
     2401 ccgacatgac cgccacagcc gccgccatag accgcctgct tccccaaacc caatgccgcg
     2461 aatgcggcta cgacggctgc ctgccctacg cacaggcagt cgcaacaggc gaagcgtaca
     2521 acctctgcgc cccgggcgga gaagccgtca ttcgggacat ttccgccctg ctcggcaaac
     2581 cccttgtcgc acctgccaaa acccaagcca aagcactcgc ccagatagac gaaaccgcct
     2641 gtatcggctg caccgcctgc atccgcgcct gccctgccga tgccattatg ggtgcgggca
     2701 aacttatgca caccgtcatc gccgacgaat gcaccggctg cggactctgc gtcgccccct
     2761 gccccgtcga ctgcatccat atgcagcccg ttgccgacac cgtcctgccc cgcgcgcgcc
     2821 gcttcagcct gtccgccgac agccgttttg ccgccgccga acacgcgcgt gcgcgctacc
     2881 tcaaacgcaa cgaacgcaaa cagcgcgaag ccgacgaacg caaggccatg cttgccgaac
     2941 gcgaagccgc cgtccgcaac gcgcgtccgc aaacacccga cacaccgaaa aaaccggcgt
     3001 ttaaccctgc cgacctcatc gccaaagcca tggcaaaagc gcaaacccaa caagaccgcc
     3061 tcgccgccgc cgacaaccgc aaagactatc aggcgaaaca gatagccgaa gcccgcgaac
     3121 gcgccgagtt gcgccgcgcc caacgcgata tgaaatacgg cagcgacagc gaaaaagccg
     3181 ccgccctcga atatctcaaa caatacaaag ccaaacagga agccgcacag aataccgcct
     3241 cctgaccctt ccttgatatg ccgtctgaag ccgcttcaga cggcatttta tcaagctctc
     3301 cgtccgccgc acccgtgccg tccgcatctt accgcccacc cttccggccg cgccgttttc
     3361 aataaaatat taattacacg ccactacaaa tttgctataa tccgccccga aaatctaccc
     3421 aacccctcga caaaggaaca aaccatgggc atcaaagtcg ccatcaacgg ctacggacgc
     3481 atcggccgcc aggttttgcg cgccatctac gactatcaga ttcaagacca actccaaatc
     3541 gtcgccgtca acgccagcgg cagccttgaa accaacgccc atctcaccaa attcgacacc
     3601 gtgcacggac gctttgaagc cgacgtatcc cacgacggcg gcaacctcat cgtcaacggc
     3661 gacaaaatcc ccttcttctc gacccgcaac cctgccgaac tgccgtggaa agaactcggt
     3721 gtcgatttgg tcatggaatg caccggcgcg ttcaccagca aagaaaaagc caaagttcat
     3781 ctggaaagcg gcgcgaaaaa agtcctcatt tccgcaccgg gcggcgacga tgtcgatgca
     3841 accgtcgtgt acggcgtgaa cgacagcgtc ctgaccgccg acatgaccgt cgtttccaac
     3901 gcttcctgca ccaccaactg cctctcgccg gttgccaaag tgttgagcga aagcgtcggc
     3961 atcgtcaaag gcgcgatgac caccatccac gcgctgacca acgaccaaac cgttaccgac
     4021 gtgcgccaca aagacctgcg ccgcgcccgc agcggcgtgg aaaacatgat tccgaccaaa
     4081 accggcgcgg caaaagccgt cggtttggtc ttgcccgaat tgaaaggcag gctcgacggg
     4141 cttgccatcc gcgtgccgac cgtcaacgta tcattggtag atttgaactt ccaagccgcg
     4201 cgcgacacga caatcgaaga aatcaacgca ctgatgaaag ccgcctcgga agcaggcgcg
     4261 ctcaaaggcg ttttaggcta caacaccctg cccttggttt ccatggactt caaccacact
     4321 accgaagcca gccacttcga cgcaacgctg accaaagtcg ttgacggcaa catgattaaa
     4381 gtcttcgcat ggtatgacaa cgaatggggc ttcagctgcc aaatgctgaa caccgcacgc
     4441 cgtatgttcg gacttgaagt gcgcccgctc aaataagcaa taaaccgtca aacaaaatgc
     4501 cgtctgaaac ccgatgtttt caagttccag acggcatttt tcatttttgg cagtgatttt
     4561 gcccggctgt cgtcatttct aattttatag tggattaaca aaaaccagta cggcgttgcc
     4621 tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
     4681 atccactata atccaccata tttgaacctg accgaagcat aagccgccat caaagcccct
     4741 tgcacgccac ttcgcgccgc ccgatttcct ctttcaacgg cacggcgttc aacatccgca
     4801 gacgttcggc aaaatccgca aacggcagca gctccgaacc gaaacggcgc atatgttccg
     4861 tgtcctgctg gcagtctatc agttccacac ccaaatccgc caaaaacggc acggcgcagg
     4921 caaacgcgat tttcgacgca tccggttgca acgcaaacat cgattcgcca taaaacacgc
     4981 tgccgatctg cacgccgtaa aagccgcccg ccaacctcgt ttcaccgctt tcatcgggat
     5041 aatggcactc gaaagaatgc gcgtgtccca tttcgtgcag cttcaaatat gccgtctgaa
     5101 actcgggcgc aatccaagtt ccgtcctgat tcgggcgcgc cgctgccgca caatgcgcga
     5161 ccacttccgc aaaacagccg ttgaccgcaa cccgatagct gccgttgcgc agcgttttcg
     5221 ccagcgagcg cggaacgtgc agacgttcgg gaacaatcac cgcacgcggg ccgaccgcat
     5281 accaaaaaaa ccaaccgtcc cgagaaaacc acggaaacac gccgttccga tacgcctcga
     5341 gcagccgccc cgcatccaaa tcgccgctca cgccgaccag cccgtcgcac cgggccaaag
     5401 cataggcagg atcgggaaag gcataattgt caggggcaag cagcggaata cgcatggcag
     5461 ttctccaaaa acacagacgg catccaaaat catgaatgcc gtctgaacaa caaaccgtcc
     5521 ggatttaacg tttgcccttg gcctgacagt cgctgcacac gccgtacata taaagcgcgt
     5581 gatcgacgat gcggtagccg ttttcctcag cgattttgtc ttgcagggct tcgatttcgg
     5641 gattgtggaa ttccgttacc tcgccgcact tcacgcagac gatatggtcg tggtggtcgc
     5701 ccttgtccaa ctcataaacc gccttgcccg tttcaaaatg gtggcgttgc agaatgcccg
     5761 cctgctcaaa ctgggtcagc acgcggtaaa tcgtcgccac accgatttcc acaccctctt
     5821 ccaacaaaat gcggtacaca tcttccgcac tcaaatgctc ttccgcatgc gtctcgaaca
     5881 aatccaaaat cttcaaacgc gggccggtaa ccttcagacc gctgtctttc agttgtgcaa
     5941 tattgttgaa tttttccata atattcaata cccctgtaaa acaatagacg ttataatacg
     6001 caatttcggc ctgtttgccc actatcgcac catagcagtt tgcaatagta aaaccgcacc
     6061 ggcggccgat gcgcggacag ggcggatacc gcttaatcgt atgattatcg ttcgctttta
     6121 taaaatattc aagcagtgtt acactaccca tcccgatttg cacagaaagg catttccgtg
     6181 aacaaaaccc tcatcctcgc cctttccgcc ctcctcggcc ttgccgcgtg cagcgtcgaa
     6241 cgcgtttcac tgttcccctc gtacaaactc aaaatcatac agggcaacga actcgaacct
     6301 cgcgccgtcg cctccctccg ccccggtatg accaaagacc aagtcctgct cctgctcggc
     6361 agccccatac tgcgcgacgc attccatacc gaccgctggg actatacctt caacacctcc
     6421 cgcaacggca tcatcaaaga ccgaagcaat ctgaccgtct attttgaaaa cggcgtgctc
     6481 gtccgcaccg aaggcaacgc cctgcaaaat gccgccgaag ccctccgcgt aaaacaaaac
     6541 gcagacaaac aataaggaac acacatgaca ccgctcaaaa tcgccatcgc cggcgcaaac
     6601 ggccgtatgg ggcgcgtcct cgtcgaagcc gtcaacaacc atcccgacac cgtcctttcc
     6661 ggtgcgcttg aacactcagg ctcagaagcc ctcgggctgg acgcaggcta cgccgtcgga
     6721 ctcaaaaccg gcatcgccat ttcagacgac gttgacgccg ttctcgcaca aagcgacgta
     6781 ctcatcgact tcacccgccc cgagcccacc ctcaaacacc tgcaaaaatg cgttgaaaaa
     6841 caagtcaaca tcatcatcgg cacaacaggc ttcgacgatg cgggcaaagc cgctatccac
     6901 actgccgccg aacaaacagg catcgttttc gccgccaact tcagcgtcgg cgtcaacctc
     6961 accttccaca tcctcgacac cgtcgcccgc gtgttaaacg aaggctacga catcgaaatc
     7021 attgaaggcc accaccgcca caaagtcgat gcccccagcg gcaccgcgtt acgcatgggc
     7081 gaagtcatcg ccggcgcgct cggcagagac ctcaaacaat gcgccgttta cggccgcgaa
     7141 ggccacaccg gtccgcgcga tccgtcgacc atcggctttg ccaccgtccg cgcaggcgac
     7201 atcgtcggcg accacaccgc cctcttcgcc accgacggcg agcgcgtgga aatcacccac
     7261 aaagccggca gccgcatgac ctttgccgcc ggcgccgtcc gcgccgcagt ttgggtaaac
     7321 ggcaaaacgg gtttgtacga tatgcaggac gtactcgggc tgaacaaccg ttaaccccca
     7381 tacaaaatgc cgtctgaaaa gatattgttc agacggcatt ttgccgacag gctccgtatc
     7441 ggcatatcaa tgtttcaaca cacgggacga tacataaagc gtcgccctat gtgtcgtcct
     7501 gatttggaag ggattacgcc cctcccaaat aaagtctgat tctaccgccc cgaaggacag
     7561 atgtccgagt ggcggggttt caaccgaaaa ggaaatacga taaagttgct tgcgcggcat
     7621 gacaagttac ggctcgttta attaaaactc tcctgatcta aaaattctaa ctctatttcc
     7681 cggcaaacta tatctatact aaaaccggtc ggaagatcat acctaccgcc tatcacttcc
     7741 agcgcacctc cataatccaa gatttcatca tcctgcttta attctccaag ttccaataca
     7801 tttttgatat ttttatcttg gttcttcgtg gcgtatccat agataaattt cagatagctg
     7861 aactgcttga atttaatttt tacccattct gaataattgg gcatagggat ctcttgagga
     7921 aagtccggat aatccacatt ctggacgatg atatggaatt catcgttttt caaagtatag
     7981 tttaatagag tgccctcata tatatacaga aattgataat ctttttttgg aatggtataa
     8041 gttgtcatat tattttacct tttcaaaaac aatgagttta agattttttg agtccaagtt
     8101 ggttctacta ctcttatgaa atttcagttg tttaaactct tcctttaaca gaccattata
     8161 ttttaacctt aatcccactc gagccagttc tgcttcattt ggaattcctc ttaaatactt
     8221 attgtttgat gtgccattaa tcacaatttc tccccctttt tttgtaatac gtgctgcttc
     8281 gggaaaataa tccattaacc gctcaacatc ccgcctcttc caaaaccgcg cgcgcggcca
     8341 aatccgccgt atcttttttc aacagcaggt gcaacgccct gaaatcctgt tgcagcgcgg
     8401 caaccttatc ggggtgttcg taccagtccg ccaacgccgc cgccagtttt tccggttttg
     8461 cttcagattg caataattcc ggcacagcct ccttacccaa caggatattc ggcaggccga
     8521 catgcggcac tttgattttg cgtttcacat aagcataagt cagcggcgaa atcttgtagc
     8581 tgatgaccat cggacgcttg cacaacgcca cctccaaagt cgccgtaccg ctcgttacca
     8641 gcaccgcatc cgccgccctg cacactgttt cagactgtct gtcgattacc gtcagcggca
     8701 atccggcaaa ctccggccgc tgcaaaactt ccgccaaacg ccgcttcgtc gcctccgttg
     8761 cggcaggcag caggaagcat gcggcaggat agcgttccaa caacaataat gccgtctgaa
     8821 aaaacaccgg cgccatatag tcgatttcgc tgacgcggct gccgggcagc agggcgaata
     8881 cggggatgcc ggcatccacg cccaaagttt gccgcgccgt ttcacggtcg tcttccaagg
     8941 gcataagctg cgccatcgga tgaccgacaa actccgcacg tccgcccgca tcgagataaa
     9001 gctgcggctc catcgggaac aggcacaaca cgcggttgac ctgatgcacg attttgccca
     9061 cacgttcccg ccgccacgcc cacaccgacg ggctgacata atgcacggtc ggaatccccg
     9121 accgtttcag cttttccgcc acacccaaat taaaatcggg cgcatcgata ccgacaaaga
     9181 catcaggttt caacgacagc aaatcccgta ccagccccct gcgtatccgt aaaatttccg
     9241 gcagccgcct gaccacttcg acaaagccgc gcaccgccag ccgctcctga tcataaaggc
     9301 tctcgaaacc ttccgccttc atcagttcgc cgccgatacc gacaaaacgc gcctgcggac
     9361 aacgcttgcg gatggcgcgt atcaggtgcg cccccaatag gtcgcccgac gcttcgccga
     9421 cactgacggc aatcaaaggg ctttttttat cagccatatt cgtctgtccc cacatacttt
     9481 cacgtcccga tgccgtctga aggcttcaga cggcatcggg gttcacattt tcgtcccgtt
     9541 ttccaacatc accgcaaact gcgccaagtc cggcggcagc tccgccttca acaccagcgg
     9601 ctcgcccgtg agcggatggt tcaagtgcag ctcggacgcg tgcaaaaaca tccgcttcaa
     9661 acccaacttc tgcaaacgac ggttcgcctg ataatcgccg tagcgttcgt cgcccgcaat
     9721 cggacagcct tgagattgca ggtggacgcg gatttggtgc gtacgacccg ttttcagcgt
     9781 tgcccgcacc aaagtcaggt gcgacagccc gacaccgtgc aaaatgccgt ctgaaaaacg
     9841 gcttaacaca cggaacaccg tgtgcgccga ctgcccgtcc tcgctgacgc gtaccatctt
     9901 ttcgccttgt gcgccggtat atttgaacag gggcagtttg acatggaaat tgtcgtccgg
     9961 cagtttgccc acccccagcg caaggtagat ttttttgggg tggtcgttac ggatggcttc
    10021 gtgaagtttg acgagcgcgc tgcgtttctt cgccaccatc aacaagccgc tcgtatcctt
    10081 gtccaaacga tgaaccaact ccaaatactt cgcctccgga cgggcgcggc gcaactgttc
    10141 gataacgccg aaactcacgc cgctgccgcc gtggacggca acgccggacg gtttgtcgat
    10201 gaccaaaagc gcatcgtctt cgtaaacaac gtcaaacgca cgcgccggta cggcggcacg
    10261 cctttcagac ggcatttcct tctccgccac gcgcacaggc ggaatccgca ccgtatcccc
    10321 ctccgcaata cggctgtcgg gtttgcagcg tttcttgttc aaccgcacct cgccggcgcg
    10381 gataatgcgg tggatatggc tcttgggaac acccttgagg atttttatca gatagttatc
    10441 aaggcgttga cccgcctcat gttcggcaac ccctatcaag ctgaccgaat ctttgcttat
    10501 ttcgtgcgtt ttcatctata atccgaacat ccgtttcagc aaaaagcgcg cccgccgcgc
    10561 ccccctgaaa cggtttactt taaaacggaa ttttatatta aaaacgcact ccgcaaagca
    10621 ttttccctgc gcctgttccc agccggcagg cgcagaacgt aatcaagttt gaattgattt
    10681 tgccgtttcg gcgcggaagt taagacggca gccgggcccc aagtcccaaa cgcaaaccgc
    10741 ccaaaacaca ggcatcacga taacaagaag aagccggccc ccatttttcc gaccgcagcg
    10801 ttcggaacgc ggcagacaaa ccgccgcatc gatctttata gccttctgta tccgaccctt
    10861 ccgcacacgg ttctccggaa cgtgcatccc tgcggatttt caactcaaaa actgattacc
    10921 ggttttatcc gaaaacatcg gaaaacccag ccttacggaa tgccgaaccg gaccggcgcg
    10981 cccgaatgcc gcccgtccac gaggtgatca tgaaaagaat gttattcaac gcaacgcagg
    11041 ccgaagagct gcgcgttgcc atcgtcgacg gacaaaacct gctggatttg gacatcgaaa
    11101 cgctgggcaa agaacagcgc aaaggcaata tctacaaagg catcattacc cgcatcgagc
    11161 cgtcgctgga agcgtgtttc gtggattacg gaaccgaccg ccacggtttc ttgccgttta
    11221 aagaagtctc ccgctcatac ttccaagact acgaaggcgg acgcgcgcgc atccaagacg
    11281 tgctcaaaga aggcatggaa gtcatcgtcc aagtcgaaaa agacgagcgc ggcaacaaag
    11341 gcgcggcgct gaccaccttc atcagcctcg ccggacgcta tctggtattg atgccgaaca
    11401 acccgcgcgg cggcggcgta tcccgccgta tcgaaggcga agaacgtcaa gaactcaaag
    11461 ccgctatggc gcaactcgac attccgaacg gcatgagcat catcgcccgt accgccggca
    11521 tcggccgcag cgcggaagag ttggaatggg atttgaacta tctcaaacaa ctctggcagg
    11581 cgattgaaga agcaggaaaa gcgcatcacg acccttacct gctctttatg gaaagctcgc
    11641 tgctcatccg cgccatccgc gactatttcc gccccgacat cggcgaaatt ctggtggaca
    11701 atcaagaagt ttacgaccaa gttgccgagt tcatgagcta cgtcatgccg ggcaatatag
    11761 gccgtctgaa actctacgaa gaccacacgc cgctgttttc ccgcttccaa atcgaacacc
    11821 aaatcgaaag cgcgttttct cgcagcgtca gcctgccttc cggcggcgca atcgtcatcg
    11881 accacaccga agccctcgtc tccatcgatg tcaactccgc acgcgccact cgcggcgcag
    11941 acattgaaga caccgcgttc aaaaccaata tggaagccgc cgaagaagtc gcgcgccaaa
    12001 tgcgcctacg agacttgggc ggtttggtcg tcatcgactt catcgacatg gaaaacccca
    12061 aacaccagcg cgatgtggaa aacgtcctgc gcgacgcgct caaaaaagac cgcgcccgcg
    12121 tgcagatggg caaactctcc cgcttcggcc ttttggaatt gagccgccaa cgcctgaaac
    12181 cagctttggg cgaaagcagc cacgtcgcct gtccgcgctg cgccggcacc ggcgtgattc
    12241 ggggcatcga atccaccgcc ctgcacgttt tgcgtatggt tcaagaagaa gcaatgaaag
    12301 acaacaccgg cgaagtgcgc gcccaagtgc ccgtcgatgt cgccaccttc ctgttaaacg
    12361 aaaaacgcgc cgagctgttt gcgatggaag agcgtttgga tgtcaacgtc gtcctgattc
    12421 cgaacatcca cctcgaaaac ccgcactacg aaatcaaccg catccgcacc gacgacgtag
    12481 aagaagacgg cgaaccgagc tacaagcgcg tcgccgagcc ggaagaagac gaatccgcca
    12541 aacctttcgg cggcgaaaaa gccaaagccg cccgtcccga acccgccgtc aaaggcgtgc
    12601 gccacaccag ccccgccccg actgccgccc ccgagaaaaa aacctcctgg tgggacagct
    12661 tcaaagcatg gctcaaacgc attttcggcg gcagcgaaac ccaagccgcg cccgctgccg
    12721 aaacctccga aaaacgcagc acggcaaacc gcagcggcag ccgcgccaac aaccgccgcc
    12781 aaaacccgcg ccgcagcaaa cgcgaaggca gcaaagtaga agtccgcgaa gtggcaggca
    12841 aaactgccgg acaggaagcg cgtgccgaca aagccgaaac gcgcaacaac ggcaaccgcc
    12901 gccgcaacga acgcggcgac cgtgccgccg aacgcgccaa cgaagcggaa atccaagacc
    12961 gcaacataca gcctgccgca accgttgcag atgccgcacc gtccgaaacc gaagtgcaaa
    13021 ccggcaaacg ccgccgcaac ggcagccgca acgaacgcgg ccaaaccgcg ctggaaaccg
    13081 ccgccgttgc cgaaacagcc gttcagacag cggaaaacac gccgtccgaa ccgcataccg
    13141 cagaagacaa aggcagcaag cccaaatccg aacgcaaccg ccgcgaacgc gacagccgcg
    13201 acgccaaaga acgccgcgag cgcaacaatc agcgcgaccg ccgtcaaaac ggcaaaaaac
    13261 gcaatatccc gtctgccgcc aaaatcgagc agtacctgaa tattcacgac actgccgaca
    13321 aagtccgttc cgccgccgca cacgttttcg gagaaaccga cgcaaacgcg ccgattaccg
    13381 tcagcattgc cgatccgctc atcgccacac ccgttcagac ggcatcttcc gccgtttcaa
    13441 acggcgacgc actggtttat gatgcggcag aaaaaatccg ccgtgccgcc gccatcctgc
    13501 ccgaaggcgc gacaccgaaa gcctcagcac aggaaatgcc gtctgaaacc gcaaccttta
    13561 cggctgcggc ggaacaggca cgggaaaccg cacaaaccgg cggactcgtc ctgatcgaaa
    13621 ccgaccctgc cgcattgaaa gcatgggcgg cacaacccga agtccaaacc ggacgcggtt
    13681 tgcgccgttc cgaacagccc aagccgtctg aagccgcaac cgtccctgcc gaagaaatga
    13741 tccaagtcga aacccggcaa ggctgaaccg acggcggcaa aaagaggttc tgttccgcag
    13801 aacctctttt ttatatagat tcggatacct gcaatgccgt ctgaaacttc gccattcccg
    13861 tgattaccga aacattccgc cattcccatg attcccgcaa cattccgtca ttcccgtgaa
    13921 aacgggaatc tagaacctca aactttcaga taacctttga atattgccgc cgcccgaagg
    13981 cctggattcc cgcctgcgcg ggaatgacgg tttagaagtt gcccgaaacc tcaaaaaaaa
    14041 accgaaaccg aacaagccgg attcccgcaa cattccgtca ttcccgtgaa aacgggaatc
    14101 tagaacctca aactttcaga taatctttga atattgctgc cgcccgaagg tctggattcc
    14161 cgcaacattc cgtcattccc gtgaaaacgg gaatctagaa cctcaaactt tcggataatc
    14221 tttgaatatt gccgtcgccc aaaggcctgg attcccgcct gcgcgggaat gacggcggag
    14281 ggtggacgat gccgtctgaa actgcgccat tcccatgatt accgcaacct ttcgtcattc
    14341 ccgccacctt tcgtcattcc cgtgaaaacg ggaatctaga acctcaaact ttcagataat
    14401 ctttgaatat tgccgccgcc caaaggtctg gattcccgca acattccgtc attcccgtga
    14461 aaacgggaat ctagaacctc taaactttca gataatcttt gaatattgcc gtcgcccaaa
    14521 ggcct
//
LOCUS       DAWHXS010000051        14493 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWHXS010000051 DAWHXS010000000
VERSION     DAWHXS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.051"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..661)
                     /locus_tag="WIE22_001176"
     CDS             complement(<1..661)
                     /locus_tag="WIE22_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ4127263.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EA"
     gene            731..1735
                     /locus_tag="WIE22_001177"
     CDS             731..1735
                     /locus_tag="WIE22_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ4127264.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(1792..2490)
                     /locus_tag="WIE22_001178"
                     /pseudo
     CDS             complement(1792..2490)
                     /locus_tag="WIE22_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
     gene            2708..4246
                     /gene="murJ"
                     /locus_tag="WIE22_001179"
     CDS             2708..4246
                     /gene="murJ"
                     /locus_tag="WIE22_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ4127265.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            4379..5335
                     /locus_tag="WIE22_001180"
     CDS             4379..5335
                     /locus_tag="WIE22_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ4127266.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            5386..6168
                     /gene="trpC"
                     /locus_tag="WIE22_001181"
     CDS             5386..6168
                     /gene="trpC"
                     /locus_tag="WIE22_001181"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ4127267.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVPLEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIEAEVGKLF"
     gene            6233..8533
                     /gene="recQ"
                     /locus_tag="WIE22_001182"
     CDS             6233..8533
                     /gene="recQ"
                     /locus_tag="WIE22_001182"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ4127268.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGKQEAIVNALAGGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNILRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPKGLAELA
                     AVYGVGEEKTARYGAAVLAVLGQNAV"
     gene            complement(8676..9101)
                     /locus_tag="WIE22_001183"
     CDS             complement(8676..9101)
                     /locus_tag="WIE22_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127269.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTSTALSRLSSKRTIL"
     gene            complement(9432..9668)
                     /locus_tag="WIE22_001184"
     CDS             complement(9432..9668)
                     /locus_tag="WIE22_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127270.1"
                     /translation="MFVNPLYADAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATL
                     LALDWQGNKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            9657..9758
                     /locus_tag="WIE22_001185"
                     /pseudo
     CDS             9657..9758
                     /locus_tag="WIE22_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(9839..10627)
                     /locus_tag="WIE22_001186"
     CDS             complement(9839..10627)
                     /locus_tag="WIE22_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ4127271.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(10629..11504)
                     /gene="bioH"
                     /locus_tag="WIE22_001187"
     CDS             complement(10629..11504)
                     /gene="bioH"
                     /locus_tag="WIE22_001187"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ4127272.1"
                     /translation="MRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKS
                     MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDAPFAQPFDIEAAAD
                     AVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTADEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGTPSALQEALDAA
                     ERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRLVVMEKAAHAPFLSH
                     AEAFAALYRDFVEGV"
     gene            11599..12096
                     /locus_tag="WIE22_001188"
     CDS             11599..12096
                     /locus_tag="WIE22_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ4127273.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            12161..12625
                     /gene="trmL"
                     /locus_tag="WIE22_001189"
     CDS             12161..12625
                     /gene="trmL"
                     /locus_tag="WIE22_001189"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ4127274.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            12636..12758
                     /locus_tag="WIE22_001190"
     CDS             12636..12758
                     /locus_tag="WIE22_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ4127275.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            12755..13474
                     /locus_tag="WIE22_001191"
     CDS             12755..13474
                     /locus_tag="WIE22_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ4127276.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSALV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNLASSASNPNLSVEKRHYEYVVKMGPFSSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            13536..13862
                     /locus_tag="WIE22_001192"
     CDS             13536..13862
                     /locus_tag="WIE22_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ4127277.1"
                     /translation="MNIKHLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            13944..>14493
                     /gene="ruvA"
                     /locus_tag="WIE22_001193"
     CDS             13944..>14493
                     /gene="ruvA"
                     /locus_tag="WIE22_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ4127278.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPKGTDVGE"
BASE COUNT         3269 a         4206 c         3846 g         3172 t
ORIGIN      
        1 cggcttcgac ggaagcggcc ttgacatacc agccggcatc gccggcggaa caggtgttga
       61 attgggtttc cgtcagtttg taatgccctt cgcccaacat ttcggcggtg cggctgacgc
      121 tttgcagccg ccgtccgcct tgttcggttt ccatacggac gttgtgcgct tccccggtct
      181 gctgctcgag attgtaggtc agggtttcgc cccgaatcag cgtaccgtcc tgttggaggg
      241 cgaaccggtc gcctgcggta acggtgtcgc ccgactggtc gtaatccgcc caatcggtat
      301 tgagggtcgt ccggttgcgt tcgacgacga cgttgccttc ggcacgcacc tgcacctgcg
      361 actgtccttc caccttgtcg gcaacaatgc gcgtatagtc ttcggggatg gatgcttcgc
      421 cgctgcctcg gacggcggct tcggttctct cggggctgcc gctttcgtta ctgcaaaaca
      481 ggcaggtcga accgaggctc aggctggtag gctgtatggg ttcggacacg ctccgaacgc
      541 ttcctccggc ggtcggattg tccgtttcct ccgccgcaac ggcatcggcg gcggcgcaat
      601 gcgtgccgaa gcagaggccc aatgccagca ccagtggttt gagtgaaaat aaacgagcca
      661 aaatcgcccc tcaagtcggt ttgccagtta gaatagcgtt tattgtaacc tgaaatgctt
      721 tagtactgtt atgcaacgac aaatcaaact gaaaaattgg cttcagaccg tttatcccga
      781 acgggacttc gatctgactt ttgcggcggc ggatgctgat ttccgccgct atttccgtgc
      841 aacgttttca gacggcggca gcgtcgtctg tatggatgcg ccgcccgaca agatgagtgt
      901 cgcgccttat ttgaaggtgc agaaactgtt tgatatggtc aatgtgccgc aggtattgca
      961 cgcggacacg gatttggggt ttgtggtatt gaacgacttg ggcaatacga cgtttttgac
     1021 cgcgatgctt caggaacagg gcgaaacggc gcacaaagcc ctgcttttgg aggccatcgg
     1081 cgagttggtc gaattgcaga aggcaagccg tgaaggggtt ttgcccgaat atgaccgtga
     1141 aacgatgctg cgcgaaatca acctgttccc ggaatggttt gtcgcaaaag aattggggcg
     1201 cgaattaaca ttcaaacaac gccaactttg gcagcaaacc gtcgatacgc tgctgccgcc
     1261 cctgttggcg cagcccaaag tctatgtgca ccgcgacttt atcgtccgca acctgatgct
     1321 gacgcgcggc aggccgggcg ttttagactt ccaagacgcg ctttacggcc cgatttccta
     1381 cgatttggtg tcgctgttgc gcgatgcctt tatcgaatgg gaagaagaat ttgtcttgga
     1441 cttggttatc cgctactggg aaaaggcgcg ggctgccggc ttgcccgtcc ccgaagcgtt
     1501 tgacgagttt taccgctggt tcgaatggat gggcgtgcag cggcacttga aggttgcagg
     1561 catcttcgca cgcctgtact accgcgacgg caaagacaaa taccgtccgg aaatcccgcg
     1621 tttcttaaac tatctgcgcc gcgtatcgcg ccgttatgcc gaactcgccc cgctctacgc
     1681 gctcttggtc gaactggtcg gcgatgaaga actggaaacg ggctttacgt tttaaaccca
     1741 atcaaaatgc cgtctgaaaa ccaagtttca gacggcattt ttcaaacggg cttactgcgc
     1801 ggctttttgt tcttcgcgta ctttgtccgc caaaaggtcg atggtgtgca taccggactc
     1861 ccagtcggca aattcgactt tatatttgcc gccgacgata accgtgggcg taccgtcgat
     1921 ttggaaggtt tcggtcagct cctgcatttt gtcggcgcgc gcctagcttt cgggggactc
     1981 gtaggcggca aggacttttt tgccgtcaaa ggcggtttgt tcgcccagcc attttttgag
     2041 gacttccggc tcttgcagct tgattttttg gttgaccatc gcatcgaaaa tatggctgtt
     2101 cgccacatct ttgctgtcgg cggcagccat atcgacggcg gcggcgaggc gtgcgagcgt
     2161 cagcatttct ttctgccaga cgacgtgttc ggtacgcagg tacatatcgt ctttaaaaga
     2221 cttggcgtgt ttgcttaaaa caggttcgag gtgggcgcag tgcggacaga aatagccgaa
     2281 aaattcaagg acttcgactt tgcctgcctg ctgttgggga atcgggttgg caaggacggt
     2341 atagttttgc ccttcgacca gccctgccgg ggcggcggct gccgaagcgg caggcgcgct
     2401 gtcggcgggg acgctggttt ggactttgct gtcgcacgcg gcaagggcga acagggcggc
     2461 aacgccgagg gcgaggtgtc tggatttcat acggctctcc gtgatgttgg aaacagatgt
     2521 tggaaatcaa tcggaatccg gctattttat tgcatttccg cgtgttgata cagtttgccg
     2581 ccggaaaagg aagctttcgt ttcgggaaac cgcttcagac ggcatcaaac ccgatgccgt
     2641 ctgaagcggt ttctgtcgta caatacgcgc cgttgcccca aacgggtacg actgttgagg
     2701 aacaatgatg aatatgctgg gagctttggc aaaagtcggc agcctgacga tggtgtcgcg
     2761 cgttttggga tttgtgcgcg atacggtcat tgcgcgcgca ttcggcgcgg gcatggcgac
     2821 ggatgcgttt tttgtcgcgt tcaaactgcc caacctgctt cgccgcgtgt ttgcggaggg
     2881 ggcgtttgcc caagcgtttg tgccgatttt ggcggaatat aaggaaacgc gttctaaaga
     2941 ggcgacggag gcttttatcc gccatgtggc ggggatgctg tcgtttgtgc tggtcatcgt
     3001 taccgcgctg ggcatacttg ctgcgccttg ggtgatttat gtttccgcac ccggttttgc
     3061 caaagatgcc gacaaatttc agctctccat cgatttgctg cggattacgt ttccttatat
     3121 cttattgatt tcactttcct cttttgtcgg ctcggtactc aattcttatc ataagttcgg
     3181 cattccggcg tttacgccca cgttcctgaa cgtgtcgttt atcgtattcg cgctgttttt
     3241 cgtgccgtat ttcgatcctc ccgttaccgc gctggcttgg gcggtctttg tcggcggcat
     3301 tttgcagctc ggcttccaac tgccctggct ggcgaaactg ggttttttga aactgcccaa
     3361 actgagtttc aaagatgcgg cggtcaaccg cgtgatgaaa cagatggcgc ctgcgatttt
     3421 gggcgtgagc gtggcgcaga tttctttggt gatcaacacg attttcgcgt cttatctgca
     3481 atcgggcagc gtttcatgga tgtattacgc cgaccgcatg atggaactgc ccggcggcgt
     3541 gctgggggcg gcactcggta cgattttgct gccgactttg tccaaacact cggcaaacca
     3601 agatacggaa cagttttccg ccctgctcga ctggggtttg cgcctgtgta tgctgctgac
     3661 gctgccggcg gcggtcggac tggcggtgtt gtcgttcccg ctggtggcga cgctgtttat
     3721 gtaccgcgaa ttcacgctgt ttgacgcgca gatgacgcaa cacgcgctga ttgcctattc
     3781 tttcggttta atcggtttga tcatgattaa agtgttggca cccggcttct atgcgcggca
     3841 aaacatcaaa acgcccgtca aaatcgccat cttcacgctc atctgcacgc agttgatgaa
     3901 ccttgccttt atcggcccac tgaaacacgt cggactttcg cttgccatcg gtctgggcgc
     3961 gtgtatcaat gccggattgt tgttttacct gttgcgcaga cacggtattt accaacctgg
     4021 caagggttgg gcggcgttct tagcaaaaat gctgctctcg ctcgccgtga tgggaggcgg
     4081 cctgtatgcc gcccaaatct ggctgccgtt cgactgggca cacgccggtg gaatgcaaaa
     4141 ggccgcccgg ctcttcatcc tgattgccgt cggcggcgga ctgtatttcg catctctggc
     4201 ggctttgggc ttccgtccgc gccatttcaa acgggtggaa agctgaccga tgccgatatc
     4261 ctttttcgga aaacgctttt tccgcgccta tattcgggcg cgcatccccc gcgtccgcac
     4321 cagccggacg ggctgccgca tcatcaatgc cgtctgaaaa cagaaaaacc gataccttat
     4381 gattttgaca ccgccggaca caccgttttt cctccgcaac ggcaatgccg atacgattgc
     4441 cgccaaattc ctgcaacgcc ccgcacccgc ataccgccgc gagctgcttc ccgacagcac
     4501 gggtaaaacc aaagtcgcct acgacttttc agacggcatt tcgcccgatg cgccgctggt
     4561 cgtgctgttt cacggtttag aaggaagcag ccgcagccat tacgcggtcg aactgatgct
     4621 cgcggtacgc agccgcggtt ggcacggcgc agtcgtccat ttccgcagct gcggcggcat
     4681 tgccaacacc gctccggtgt tctaccactt gggcgatacc gccgaaatcg cctttacttt
     4741 ggacacgctt gccgcgcgtt accgtgaaat atacgccgtc ggcgtatcgc tgggcggcaa
     4801 cgcgctggca aaatatttgg gcgaacaggg cgaaaacgcg ctgccgcaag ccgccgccgt
     4861 catctccgca cccgtcgatg cagaggcggc aggcaaccgc ttcgacagcg gcatcacacg
     4921 gctgctctac acgcgctact tcctccgcac actgataccc aaagcacggt cgctccaagg
     4981 ttttcagacg gcatttgccg cagggtgcaa aacactgggc gagtttgacg accgcttcac
     5041 cgcaccgctg cacggctttg ccgaccggca cgactactac cgccaaactt cctgcaaacc
     5101 gctgctcaaa cacgttgcca aaccgctgct cctgctcaat gccgccaacg accccttcct
     5161 gccgcccgaa gcgctgccga caccggacga agtgtccgaa gccgttaccc tgttccagcc
     5221 ggcacacggt ggtcatgtcg gctttgtcag cagtaccggc ggcaggctgc acctgcaatg
     5281 gctgccgcag accgtcctgt cctatttcga cagcttccgc acaaacaggc gttaacggtt
     5341 tgatgctaat attccccctt tccccggacg aatacggaac acaatatgac cgacatcctc
     5401 aataaaatcc ttaccgccaa ggcacaggaa gttgccgccc aaaaagccgc cgttcccctc
     5461 gaacacatcc gcgcacttgc cgcagaagcc gcgcccgtcc gaagcttcat cggttcgata
     5521 cgcggcaaac accgcctaaa cctgcccgcc gtcattgccg aaatcaaaaa ggcaagcccg
     5581 agcaaaggcc tgatccgtcc ggacttccga ccggccgaga ttgcacgcgc ctatgaaaac
     5641 gccggagcgg cgtgtttgtc cgtactgacc gacgaaccct atttccaagg ttcgcccgaa
     5701 tacctcaaac aggcgcgcga agccgtatcg ctgcccgtct tgcgtaaaga cttcatcatc
     5761 gacgaatatc aggtttatca ggcgcgcgcc tggggggcgg atgccgtcct gctgattgcc
     5821 gccgcactgg aacgggaaca attggaacgc tttgaagcgg tggcgcacga attgggtatg
     5881 accgtcctgc tcgagctgca cgatgaaagc gaattggaaa aatgccgtaa cctgaccacg
     5941 ccgttgtggg gcgtaaacaa ccgcaacctg aggacttttg aagtctccct cgaccaaacc
     6001 ctgtcgctgc tgcccgcact ggaaggcaaa accgtcgtta ccgaaagcgg cattacgggt
     6061 aaggcggatg tggaatttat gcgggcgcgc ggcgtgcaca ccttcctgat cggcgaaacg
     6121 ttcatgcgtg ccgacgatat tgaggcggaa gtgggcaaac tcttctaaat cccgatttca
     6181 gacggcatat tgccgccgac cgatcaacat ccttaccgag cacggaacca tcatgcaccg
     6241 accgaccgcc aaacagattt tgcacgaagt attcggttat cccgaatttc gcggcaagca
     6301 ggaggctatc gtcaatgctt tggccggcgg cgagagtttg atggtgctga tgccgacggg
     6361 cgggggcaag tctttgtgtt accagattcc ggcgctgatg cgcgaaggcg tggcggttgt
     6421 tgtatcgccg ctgattgcgc tgatgaacga ccaagtggcc agcctgcatg tggctgggat
     6481 tgaagcggcg gcagtcaaca gcggcacatc ggcagatgag gcgcgcgaga ttgccgacaa
     6541 gcttgcccaa ggccgtctga aactgcttta tgttgcgccg gaacgcttgg ttaccgatcg
     6601 ctttttgcgt tttctcgacc aacaaaccgt cagcctgttc gccattgacg aggcgcattg
     6661 cgtcagccag tggggacacg atttccgccc cgaataccaa cagctcggca tccttgccga
     6721 acgctatccg aacgtcccgc gcatcgccct gaccgctacc gccgatgccg ccacgcgcgc
     6781 cgacatcaag cattatctgc acttggacga cgcgcccgaa tttgtctcca gcttcgaccg
     6841 tccgaatatt tattatcagg ttatcgaaaa aaacaacggc aaaaaacaac tgcttgaatt
     6901 catccgcaaa gaaatgacgg ggcaaagcgg cattgtgtat tgcttaagcc gcaaaaaggt
     6961 tgaagatgtg gcgcggtttt tgcgtgaaaa cggattaaac gcgattccgt atcatgccgg
     7021 tttgagcatg gacgtgcgcg aggaaaacca acgccgcttt acgcatgaag acaatattat
     7081 cgtggtggcg accgtggcgt tcggcatggg catagacaaa cctgacgtgc gctttgtcgc
     7141 ccatctcgat atgccccaaa gtgtcgaaca tttctatcag gaatcggggc gcgccggccg
     7201 ggacgggctg ccagccgtga gctggctgtg ttacggcttg aacgattggg tgttgctgcg
     7261 cgaacggatt gccgaaggca acagcgacga ggtgcaaaag caaatcgaaa tgcaaaaact
     7321 cgatgccatg cttgccgtct gcgaaaccgc cgcctgccgc cgcgtactgc tgctcaaaca
     7381 tttcggcgaa gcatccgaac cctgcggcca ttgcgacaac tgcctgcatc cgcccgtacg
     7441 gtttgacggc acggtgttgg tgcaaaaatt actcagctgc gtgtaccgcg ccggacaacg
     7501 ttttgccgcc ggttacatca ccaacatttt acgaggtaaa agcgacgatt ggatacgcgg
     7561 caaccggcac gaacaactgt ccacattcgg catcggtacg gagttgtccg acaaagaatg
     7621 gcgcagcgtc atccgccagt gtatcagcct cggctacctc accgtcaaca ttacccgata
     7681 tcaggcattg caactgaccg aagccgccaa aaaagtcctc aaaggcgaaa ccgaagtgat
     7741 gctgcgtccg ctcaagcgcg acaagcccgc cgcccgcacc ctcaaagaca actggctgcg
     7801 taccgaacgc gaagaacgcc tgtggcaggc attgcgcgtt tggcgtatga aacaggcaga
     7861 agccgaagtc atccccgcct atatgatttt cggcgacaaa accctgcgcg accttgtcga
     7921 aaaaatgccg caagacctca acggactgca cgacatctac ggtttgggcg aagccaaaat
     7981 cgaccgtttc ggacacggca tcctcgaagt ctgccgcaac accgccggct tcagtcgcga
     8041 cgcggtcatc cgtccgcaaa ccgaacgcga acaacaactg cgtcaaaaac tcgaggcctg
     8101 gcggtatgaa caggcaaggg cggaaaactg cgccctgcat gccgtcctct ccgacgaaag
     8161 ccttgccgat atgcttgccg ccacgcccga aaccgaaacc gacctcgaag gcgtacacgg
     8221 cttgggcagc gtacgcgccg ccaaatacgg acgggacatc ctcgccgtct gccgtccgtt
     8281 ttcagacggc atcgatgaag ccgccaaacg caaacgccgc ctgatgcgcg ccctgatcca
     8341 atggtgcggc gaaacggcaa aacacgaaca gtccgaaccc taccgcattc tcagcaaagc
     8401 cgccctgcgc gccattgccg ccaaacagcc gaagggtttg gcggagcttg ccgccgtata
     8461 cggcgtgggc gaagaaaaaa ccgcacgtta cggtgcggcg gtgttggcgg tgttgggaca
     8521 aaatgccgtc tgaaaaccgt taccaagttt cagacggcat tgcctctatt taaaaattcc
     8581 tgtttttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaga
     8641 tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta
     8701 aggcgagaca acgctgtact ggtttttgtt aatccactat catatagatt tttatgccat
     8761 ttggtcagaa acagcgaaga caggcaggga aacgccttca gttccatcgc gtcttcaaaa
     8821 tcatcccaaa catcgctcaa attctgtttg gatatgccgt attcccgtcc ggcaaacatc
     8881 acggtctttt tgcttttggc ggcttttttg aatgcctttc tctgtttttc gggtatctgc
     8941 tgccatctca actgacggta cacgtcgtag ccgtcgcccc aaaaagaggc ataccgttgc
     9001 gtgtcttcgc cgaccagcgg cgcggtattc ccaacccgta catatcccgt tggcacgaca
     9061 gcacttgcct tatacatgtg cagaccgcgt tcccaataca tattcaattg aatcgctcat
     9121 aaatttttgc tccaaaattt aaagttttac tatagtagaa ataaatatca aaactgaaaa
     9181 tattactgaa aaaattttat attgtttaac ttttctttta tacgaattcg cctttgttga
     9241 aattaattga taatcaaacc agcaagaaat aattccataa taaaacattc caatccaata
     9301 ataaaattca ggcctcttag aattagtaaa aattttaaat ccataatctc tttcaaaagt
     9361 agctattata caagtaaaca atatcaccaa ccctaaacag tccaacataa aaatatagtt
     9421 taattttttg ttcatacaat atttccaata ttattcttta agtctttgta aagtggtttc
     9481 aaatccgcca gctcctccgc cccaagcggt ttgttgccct gccagtccaa agccagcagg
     9541 gtcgcatgtg tttttttgcc accgtgttct tcaactcggg taacctggca ggcagtgtac
     9601 tgcttcagtc tgatgttata gacgcaatat acgtctccgg catcagcata tagtggatta
     9661 acaaaaatca ggacaaggcg gcgaagccgc agacagtaca aatagtacgg caaggcgagg
     9721 caacgctgta ctggtttaaa tttaattcac tatacatcgg catttccttt agttctgtcg
     9781 gttttcacaa acaaatgccg tctgaaactt ggaaacggct tcagacggca tctgcacatc
     9841 atctcttcgg aaaaaactgc accacgttct ccccttgcgg cagcatcagt tttttaacgg
     9901 cgtgtccgta cactgtttcc agcgtaatac tcaaaccgcc ttcccgttca aatattgtcc
     9961 cgacacacga acgcgcggcg ttttcatcgt tccacgccat cgcccgccaa acgcccagcg
    10021 tgtccatatc cgcccaaaac gtttccgcct ttttgtaatc caacaccagc ttcgccgtat
    10081 cggtaacggg gtcgtaaaag ccgttttcag caagcatatc gcccaagtcg tgcatatcgg
    10141 gaaacatcac gctgcggctt tcaatgccgt tttctttcag acggcatttc agttcggtaa
    10201 aagtgtcgcg cccgaagcag gtaaaaaaca gcagcccgtc cgtcttcaag gcgcgcgccc
    10261 agttgtgcag cacaggaagg atttgttccg ccgccaacag tccgagattc gaccacaaca
    10321 tatcggcaca cgcttcgggc agcggcgcga tcggggattg gcagtgttgc accacgccct
    10381 tacccgtaaa cctttgccaa aaaccgcctt tgcgggcggc agcggcagcc gccaaaaaat
    10441 ccgcacggga atcgtattct tcaaataccg cctgcggata gcgtttcgcc agcaggctgc
    10501 ggctgatgtc cgcatccgca cctgcaagca ggatatactt gggcgcgttg cggacgagtg
    10561 tcaggcgttg gtcggcatat tcggcgagat ggcggtgaac ctgccagcat ttgtcctgcg
    10621 gattcatgtc aaaccccttc gacaaagtcg cggtacagcg cggcaaacgc ttccgcatgg
    10681 ctcaaaaacg gcgcatgcgc cgccttttcc atcacaacca gcctgctgcc tttcaaacgg
    10741 cggtgcagat actcgcccat gcgcggcggc gtaatcgcgt ctttgctacc gaacaccagc
    10801 agtaccggaa catctatctt gtccaacaaa tgccgcgcat ccgccctttc cgccgcgtcc
    10861 aaagcttcct gtaaggcgga gggcgtgccg cagcgcgcca aatcgggcag gattctgcct
    10921 atgattccgt ccgcatcagg cgtgtgcaaa agctggagtt gtagaaactg tttgatatgt
    10981 ttggcataat ccgttcggaa cgcgccgacc attttgccca atgcaggcgc ggcaagccct
    11041 tcgggatagt cttcgtcagc cgtcagccgt gcgaaactcg ccgtcaggca gagcgaacgg
    11101 actttgtcgg gatggcgcgc cgccagatac agcgcgacca atccgccgag cgaccagccg
    11161 agaatgtcgg cgggcgtatc gatttgtgcg gcaacagcgt cggcggctgc ctcaatatcg
    11221 aagggttgcg cgaagggcgc gtcgccgtgt ccgggcaaat cgacggcgga caccggccac
    11281 gttgcaggca ggcgcggcat caaatcgtcg aacacgtggc ggttcgcccc ccaaccgtgt
    11341 atcaggtaaa cttttttgac ggcatcaggc atggattttc tctcttgttg gcggcgcatc
    11401 gcagacgcgc ccactatcag gcgttgcgta ttatgccacg gttcgtccgg cgtttcagac
    11461 ggcatctgcg ccggctgccg cgacgatttg gcggcatacc gcaccgacgc ggcaaacagc
    11521 tgccccctgt gtttcatgca cgttcaaggc ggcgcggtgt gcggcggctg tcagaaaaaa
    11581 ccgcccgcat tcgaccggat gtgggcttcg ctgcattacg aaccgcccgt cagcaatatg
    11641 atacacgcgc tgaagcactt ggctgatttg agcatggtgc agccgctggc agacctgatg
    11701 atgcagaatc cgcccgaccg gcttgcagac gaatgtttcg atttcgtcct gcccgtaccg
    11761 ctaagcaggg agcggctgct gcaacgcggg ttcaaccaaa gtgaaagcat cgtcgggctg
    11821 ctggcacaac gctacggctg gcagatactg ccccgacaca ccgttttccg acaccaccgc
    11881 ccgccgcaaa gcacgctcaa aggcggcgaa cggcggcgaa acatcaaaaa cgcctttgaa
    11941 atccgcacac cgataccgga aaactgtaat attttgttaa tcgacgatgt ctttaccacc
    12001 ggcgcgacgc tggacgaact ggcaaagacg ctgaaaaaat cgggcgcaaa ccgaatctgc
    12061 tgctggacgc tggcacgcac gccaatgaaa aaataactaa attttttgac accccatccg
    12121 ccttgtggca aggttacgcg cctataagtg attgatattt atgtttacca tcgttttata
    12181 ccaaccggaa atcccgccga acacgggcaa tatcatccgc ctgtgcgcca ataccggcgc
    12241 ggatttgcac cttgtcaaac cgctcggctt cccattggat tccgccaaaa tgaaacgcgc
    12301 cgggctcgac taccacgaat tcgccagcct gacggtgcac gaaaacttcg acgactgcct
    12361 caaggcactc gcaggcaggc gcattttcgc cctgaccacc aaaggcacgg cgcgccccga
    12421 tgaaaccgcg tttcagaaag gcgatgtttt actgttcggg ccggaaacgc gcggactgcc
    12481 tgccgacatc ctcgacagcc tgcccgccgc gcaaaaaatc cgcctgccga tgcagcccgg
    12541 cagccggagt atgaaccttt ccaataccgt tgccgtgatt ctctttgaag cgtggcggca
    12601 acacggttac gcaggcggcg tttgaacgca agttcatgcc gtctgaaaac ctatccggac
    12661 acattccgaa ccgccgtccg cactgtacgg cggttcggaa cgtatccggc gggcatgatg
    12721 cccattcgtc ttcaactcaa gaacggaaca cgttttgact ttaacccgaa aaaccctttt
    12781 ccttctcacc gccgcattcg gcacacactc ccttcagacg gcatccgccg acgcagtggt
    12841 caaggcagaa aaactgcacg cctccgccaa ccgcagctac aaagtcgccg gaaaacgcta
    12901 cacgccgaaa aaccaagtcg ccgaattcac gcaaaccggc aacgcctcgt ggtacggcgg
    12961 caggtttcac gggcgcaaaa cttccggcgg agaacgatac gatatgaacg cctttaccgc
    13021 cgcccacaaa accctgccca tccccagcta tgtgcgcgta accaatacca aaaacggcaa
    13081 aagcgtcatc gtccgcgtca acgaccgcgg tcccttccac ggcaaccgca tcatcgacgt
    13141 atccaaagcc gccgcgcaaa aattgggctt tgtcagccaa gggacggcgc acgtcaaaat
    13201 cgaacaaatc gtcccgggcc aatccgcact ggttgccgaa aacaaagaca tcttcatcga
    13261 cttgaaatct ttcggtacgg aacacgaagc acaagcctat ctgaaccaag ccgcccaaaa
    13321 cctggcttca tcggcatcaa acccgaacct atcggttgaa aaacgccatt acgaatatgt
    13381 cgtcaaaatg gggccgtttt cctcgcagga acgcgccgcc gaagccgaag ctcaggcgcg
    13441 cggtatggtt cgggcggtat tgactgccgg ctgacggtta tttgatacct tattaatatc
    13501 cacattttcc aacccacgat ttacaaaggc aaaatatgaa catcaaacac cttctcttga
    13561 ccgccgccgc aaccgcactg atgggcattt ccgcccccgc actcgcccac cacgacggac
    13621 acggcgatga cgaccacgga cacgccgcac accaacacag caaacaagac aaaatcatca
    13681 gccgcgccca agccgaaaaa gcagcgttgg cgcgtgtcgg cggcaaaatc accgacatcg
    13741 atctcgaaca cgacaacggc cgtccgcact atgatgtcga aatcgtcaaa aacggacagg
    13801 aatacaaagt cgttgtcgat gcccgtaccg gccgcgtgat ttcctcccgc cgcgacgact
    13861 gaatttgata caatccgtgc cgtctgaagc ccgaaccggt ttcagacggc attttgcacc
    13921 cgacacttca ggatccttca tacatgatca gcagactgac cggcaaactg gttgaaaaaa
    13981 accctccgca aatcgtcatc gatgtcaacg gtgtcggcta tgaggccgac gtatcgatgc
    14041 agaccttcta caacctgccg cccgtgggtg aaagcgtaca actgtttacc cagcttatca
    14101 ttcgggaaga cgcacatctt ttatttggtt ttgccactgc ggaagaacgc aagaccttcc
    14161 gccaactgat taaggtcggc ggcatcggcg cgaaaacggc tttgggcatt ttgtcagcga
    14221 tgacggcaga cgagctggcg cgggcggttg cagaagaaga tgtcaaacgc ctgtcttccg
    14281 cgccgggaat cggcaaaaaa accgccgaac gtatggtctt ggaactgcgc ggcaagctgg
    14341 tcgcgcatac ggtaacggac gggctgtttg ccgccgcacc cgccgccgac gaaacggaag
    14401 acatcgtcag cacactgctt gcactgggtt acagcgaacg cgaagcaaaa gcggcggtca
    14461 aaggcgttcc gaaagggacg gacgtgggcg aag
//
LOCUS       DAWHXS010000052        14450 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWHXS010000052 DAWHXS010000000
VERSION     DAWHXS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.052"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(35..1429)
                     /locus_tag="WIE22_001194"
     CDS             complement(35..1429)
                     /locus_tag="WIE22_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ4127279.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(1503..2843)
                     /locus_tag="WIE22_001195"
     CDS             complement(1503..2843)
                     /locus_tag="WIE22_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ4127280.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLQLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKDQ"
     gene            2986..3612
                     /locus_tag="WIE22_001196"
     CDS             2986..3612
                     /locus_tag="WIE22_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ4127281.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3676..5094)
                     /locus_tag="WIE22_001197"
     CDS             complement(3676..5094)
                     /locus_tag="WIE22_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ4127282.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            5421..6149
                     /locus_tag="WIE22_001198"
     CDS             5421..6149
                     /locus_tag="WIE22_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ4127283.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6208..6696)
                     /locus_tag="WIE22_001199"
     CDS             complement(6208..6696)
                     /locus_tag="WIE22_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ4127284.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(7455..7919)
                     /locus_tag="WIE22_001200"
     CDS             complement(7455..7919)
                     /locus_tag="WIE22_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ4127285.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            8244..9302
                     /gene="alr"
                     /locus_tag="WIE22_001201"
     CDS             8244..9302
                     /gene="alr"
                     /locus_tag="WIE22_001201"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ4127286.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(9366..10721)
                     /locus_tag="WIE22_001202"
     CDS             complement(9366..10721)
                     /locus_tag="WIE22_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ4127287.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(10736..11008)
                     /locus_tag="WIE22_001203"
     CDS             complement(10736..11008)
                     /locus_tag="WIE22_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ4127288.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(11134..11871)
                     /locus_tag="WIE22_001204"
     CDS             complement(11134..11871)
                     /locus_tag="WIE22_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ4127289.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(12152..13051)
                     /gene="prmB"
                     /locus_tag="WIE22_001205"
     CDS             complement(12152..13051)
                     /gene="prmB"
                     /locus_tag="WIE22_001205"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ4127290.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            13196..13789
                     /locus_tag="WIE22_001206"
     CDS             13196..13789
                     /locus_tag="WIE22_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127291.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            13901..>14450
                     /locus_tag="WIE22_001207"
     CDS             13901..>14450
                     /locus_tag="WIE22_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ4127292.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIATAAMKA
                     AIPDYMKFGEGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVAKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKGAEAVNNDTEHNAT
                     YFSHFPSKQKSTGGSRCPFLYRF"
BASE COUNT         3806 a         3776 c         3481 g         3387 t
ORIGIN      
        1 ttaaaccggt acagcgttgg ctcgccttgc cgtactattt gtactgtctg cggctcgccg
       61 ccttgtcctg atttaaattt aattcactat attttcccga caggcggatt tgaagtttct
      121 ccacatatcc ggcgcgttcg gcgcggctgg tttcggggcg gtgcgtattg agcgacgacc
      181 atttccaatg actgcgggct ttgttgagtt cgggcgggag tctggcggcg tcccacgggg
      241 ctttgcggct gtgcagctcg atatccgact gtgccgcgtg tccgcgcgtt tgcaggacgt
      301 ggagcaaatc gagggcgcgg gcggcgagca gggtcaaggt ttcagggtcg gtgtgcaggg
      361 tttggcggcc agcgagtttg tcggaaatgg tgcgggtatt gggcaggatg ccgcccaaaa
      421 agccgccgat tgccgtaccc aatccgagcg agccgccgag tgtggcgatg tccagcccca
      481 agccgatgag cgcgccggtt gccgcgcccg tgccggtgcg gatgccgtat tgtttgagca
      541 attcgctgtc gaacgggtct tggcggaagg cttgcggcat ccagtcgccg ccgtcgattt
      601 cgctgtggta gaaacggtag agggcaaaca gccgctgctg catctgccgt tcgagttggc
      661 gtatttccgc ctgcatggtt tgcagcacgg tggcggtatc ctcgttttcg tccacttcct
      721 gcctgaaggc ggcggcatca attaaaaagt cggcgatttc gcggcgcgct tcgccgtcca
      781 gccgctgcca ttcgcgccgg cgcatggctg tcaggcggtc aagtgtgctg cgttcgggca
      841 acatggtggc gaggttttcc cacaggcgta gttcgccttc aaaatcaaag gcgacggtgt
      901 cgaaccctgc gaaaacgtgc aggtttctcc tcgccagcat ggttgtccac gattcgggag
      961 gctgtccgcc ggtaaagttg aacacgggca taaccggttt ggcacaccat gaaaggatgg
     1021 tcaactcgtc cctgtatttg tcgaggacgg gttcgcgcgc gtcgatgacg tacattgcca
     1081 tatcgctttg caagacttgc cgtaagactt tggcttcctg attgaaatca tggtgcgcgc
     1141 cgtggctgcc gagaaactgt tgcagccgtt cgatgccgtc tgaacgattg tccgtatggt
     1201 tttccagcca ttccagcacg ccgcctgcgt cttcgagtcc gggcgtgtcg tacaggaaaa
     1261 ccagcgtgtc tgcgccgtcg ctgatggcgg cttcttcgac atgacgcgtg gtcgatgggg
     1321 cgtttctgac ttcgccgaaa ccgctgtcgc gcaaaagggt acgcaggagc gaggttttgc
     1381 cggtgttggt atgcccgacg acggcgaggg aaaggggttg tttgttcatg atgtttttga
     1441 agaatggatt ttcagacggt cttttttcag aatggcggct taacagaaca tttcaagtga
     1501 gtttattggt ctttcaaacg gccttcctgc gccgccctgt caggctcaag ccacgccgcg
     1561 ccgcattcgg tcagcgcgtt acgccaatgt tccagctttt ccgaaaggtc gtctgaaagc
     1621 ccctgttccg ccaaaagctg caccaccgcg ccgccctgcg ccgcttccga aagtcggacg
     1681 atctgccgca acacgccgcg gtcgggcaca gtttgggcgc gcacgccgat aagcagttgc
     1741 gccggtttct gcttcagctc tgtctccagc gcggcaacct gttcccgatt ggtggcaacg
     1801 cccttatcca gccattcctg cgccagcctg ccctcgaacc attcgccgtc ctgccactcg
     1861 gtctccagca tgaccgccca tttcggcgca tcgttcaaga cgattttcgg cgaaacggcg
     1921 gacacggttt cccgacgcgt atccgcatcg gtaattttgt tctgccagcg gcggatgacc
     1981 gcctgataat agggtttttc caaatccaag ccgtttttgc ttgttttcaa aaggatttta
     2041 cacaccaccc aagccagcag gcgcggcagg atgccgtagc aggcgatact gccgaccagc
     2101 agccccgacc aagcccgcgc atcggcaata ttgccgttca gacgaccttc gatgaccgct
     2161 cgcgcatcag gcacgggaaa acccagtttc gcaggcagcc atgccaacat ttccaccagc
     2221 cgtaccgaag acgaatcgcc caacagcgtg ctttcccagt tgaacgtata ttgccgcacc
     2281 aaaagcagca acaataccga caccagcatt ccgagcagcg tgcagagcca caggctgtgc
     2341 gacgttgcgc ctattttcca acgtaccgaa ggttgccgcc actcgtccgc atacagctgc
     2401 aacaccgcct gattgacagg gtctttgccc cgaaaccacg tcgccggact gctgaaaaaa
     2461 cgccccactt tcacgcgcag gaacaacatt gccaaccata ctgctaacat cagcgtattc
     2521 atgcccaaca cgcccgccaa aatcaggaag aaattcagcc cctgattgtc catcagaaga
     2581 taagtgcctg aaaatccggc ggtaaacatc atcgatgccg ccaccaccca caaccagaac
     2641 gaccccacac gcacacgttc caacgtgtcc cgcagcatac ggttcctgtc gatcatctcc
     2701 gcccgacgga tgattttttc ctccgtactg ccgtccacgc ggcgcaaagc ctccgtcgcc
     2761 tgcacgggat cgccgctgaa aataaagccg ccttcttcca aaatacggac cagctcaacc
     2821 agttttcggg atggattcaa cataaaatgc cgtctgaaaa taaaaaacag attttaacac
     2881 acgaattttc aagaatattc acagtgtagg caaagagtaa atcccacaca gaggcaaaag
     2941 tatcggcgta aactgactgc ctctactttc ccgaaagatt gtgcgatgta tacaggcgaa
     3001 cgcttcaata cttacagcca tttgagcggt ttgattctgg cggcggcagg tttggtgctg
     3061 atgctgctga aaaccatagg acacggggac ggctaccgta tcttcagcgt atcggtttac
     3121 ggcatcagcc ttcttctgct ctatttgagt tcctcgctgt accacggaat tgcagccgga
     3181 aaactgaaaa gcattttgaa aaaaaccgac cactgcatga tttatgtcct gattgccgga
     3241 agctacacac cgtttgcact ggtttctttg agaaacgggc cgggctggac ggtattttca
     3301 ctgtcctggc tgctggcggc tgcaggaatc gcacaagaac tcaccatcgg acggaaaagc
     3361 gaaaaacgtc tgctgtctat tgtgatttat gtcgtcatgg gttggatggt cttggcggta
     3421 atgaaatccc tgacagcctc actcccgtcg gcaggactgg cttggctggc ggcaggcggt
     3481 atgctgtaca gcgtcggcat ttactggttt gtaaacgatg aaaaaatccg acacgggcac
     3541 ggaatctggc atctgttcgt attgggcggc agcatcaccc aatttgtcag tgtgtacggt
     3601 tatgtaatct gaatgccgtc tgaaaaacaa aacctcccga tcctgaagat tgggaggttt
     3661 tctgtttgcc ggacatcagc ccttgtcgtg gaactcgtgg aattcatacg gataagacaa
     3721 atcccgaccc gcttttttct gtgccaaata atcatcataa atggcacgga tttccttacg
     3781 caacaaaaac agggctatca agttcgggat aaccataaag ccgttgaaca tatcggacag
     3841 gctccaaacc aaatcgactt tgccgagcgt acctaaaacg atggcaagca gaaccaatgc
     3901 gcgatagatg cccaagtgtc ttcccctgaa aagaaaacgg atgttggact caccgaaata
     3961 ataccagccg ataatggtcg tcaaggcgaa gaaggtcaga cacacggcaa gcaattgcga
     4021 accgaagccc ggaaatgcct tgttaaaggc aaattgagta accgccgcgc cctgttcgcc
     4081 cgaaaggttg gcatcggtca gcaggataat cagcgcagtt gccgtacata ccaaaatcgt
     4141 atcgataaac acaccgacaa atgccgccat accttgctgc acaggatgct tcacatccgc
     4201 agtcgcgtgg gcgtgcggag tcgaacccat ccccgcctca ttggaaaaca gaccgcgcgc
     4261 cacgccgaaa cgtatcgctt cgcgcatacc gatacccgcc gcaccgccca aaacggcttc
     4321 gggattgaag gcggcggtaa agatgtggtt gaacatcggc acaatatggt cggaaaattc
     4381 aaacaggata acgacggcgc acaaaatata aacaaccgcc ataaacggca cgacaaattg
     4441 ggcaatattg gcaatacggt tcacgccgcc aatcacaacc atgcccgcaa ggacggcaag
     4501 cacaataccg actgccaaag aaggcacatc aaatgcaatg gtaacggcag aagcaatgga
     4561 gtttgcctgt gtcgcattac cgataaagcc caatgcgata atcaacgcaa tggaaaagaa
     4621 accggacaaa aaacgcgccg ctcccctgcc gattttcgga gtcagaccgt gggtgatgta
     4681 gaacgccggc ccgccgatgt atttgccgtg gctgacgacg cggtatttct gcgccagcag
     4741 tgcctccgca aaaatcgtgg acatccccaa aacggcagaa acccacatcc aaaaaatcgc
     4801 gcccggcccg cctgcggtga tggcggtcgc cacgccggcg acgttgcccg taccgatttg
     4861 cgcggatatg gcaaccgcca acgcctgaaa ctgcgataaa gacttgtcgt ctttatcgcc
     4921 tttggcaaac aagccgccga atacggattt gaatcccgcg cccagcttgg taatctgcgg
     4981 cgcaccaaga tacagcgtaa aaaacaggcc gatacccaaa agcgcgtaaa tcagcaggta
     5041 gtcccaaagg aaccgattga ctgtacccac cagaacagac aatatatttt ccataaaata
     5101 aaccttatct tacaattaaa atgactgcct tccaaaagac attccaataa ggaaacacgg
     5161 cgagcagacc gtatttgccg caacagatgc cttaaattgt caacaatcgg ggagaagctg
     5221 cgccatcata ccgtaaaata tcgttaatta aaacatttct ttatttttaa gcggaaagcg
     5281 gaggaaatcg ctttcagaca gcatagacaa cggcacggca taaaacagga tattttgggt
     5341 acttgcaact tatgttaaaa tgccgaccgt aaaaaatctg acaaaaacag attaattatt
     5401 tgaaataaga aaggaaattt atggcaggcc atagcaagtg ggcgaatatc cagcataaaa
     5461 aagcccgtca ggatgccaaa cgcggcaaaa tcttcacccg tttaatcaaa gaaatcaccg
     5521 ttgcggcgcg tatgggcggc ggcgaccccg gtgccaaccc gcgcctgcgc ctggctttgg
     5581 aaaaagcagc cgaaaacaat atgcccaaag acaatgtgca acgcgccatc gataaaggta
     5641 cgggcaactt ggaaggcgtg gaatacatcg agttgcgcta cgaaggctac ggcatcggcg
     5701 gcgcggcttt gatggtggac tgcctgaccg acaacaaaac ccgcaccgtt gcggacgtac
     5761 gccacgcgtt taccaaaaac ggcggcaact tgggtaccga cggctgcgtg gcgttcaact
     5821 tcgtgcatca gggctatttg gtattcgaac ccggcgttga cgaagacgcg ctgatggaag
     5881 cggctttgga agccggtgcg gaagacgtgg ttaccaacga cgacggttcc atcgaagtca
     5941 ttaccgcgcc aaacgattgg gcgggcgtaa aatccgcttt ggaggcggca ggttacaaat
     6001 ctgttgacgg cgacgttacg atgcgcgccc aaaacgaaac cgaactctcc ggcgacgatg
     6061 ccgtcaaaat gcaaaaactg attgacgcgc tggaagactt ggacgacgtg caagacgttt
     6121 acacttccgc cgtattgaat ctggactgat acgcagcaca gcagacatac aataaaatgc
     6181 cgtctgaacc tttcagatgg cattgattta tttagccctt gcccacgccc accaaaccgt
     6241 caggacaacc gccagaaaat acgccgcgct ccaaacaggc aaagcaaccc ctaacagata
     6301 atccggttcg gcacaatttc cgaacccgcg cacgacaggc tcgaaccaat caaacaaaga
     6361 ccagcccttc aagcggaacg tccacggcgc accgcacgaa ggagccgtac ccggcggcag
     6421 cgactgcaac cacaactgat atgccgcaac agaaataccc gtaacggccg gaatgctgat
     6481 aaagacagca ccgaacaaac cgcccgccct tcttcttggt ctgcacatca ggacaattgc
     6541 cgtacacaat gcggttgcca aaacgcataa ccgctgactg atacacaaaa cgcaaggctc
     6601 catacccaaa acatactgtg ccgccaaaga accggcaaat gcacagaccg aaacggcaaa
     6661 cagcagccaa acggcttttc taaataacgg ggtcattttc tcaacacacc aatcaaaata
     6721 ccgatatgcc gattttgctg gatatatccc gagatggcaa gggacgaatc gacaccggct
     6781 caggtgcgga cgatttcggt tttccccgat atgcacaaaa cctgacaagg gacaaaacgg
     6841 cgatttgccc gcacaatgcc cacaaaaaaa tacatccgga ggatttgaat ttcagcaaat
     6901 ttagcgaatc aaaagtttat ttcaatgaaa tcatatgatt tttttgaata agcggattga
     6961 tgggtttttg aaggaatttg ttaccggata gccatcgggc aagttttttg caaaatttga
     7021 atcggtcggg taaattttca aaaaataatt gacagcggat aagaaacggc ggataattcc
     7081 cgccgtcgag ttgcttgatg cagcttgatt tttctcctct atttctcctt tgtagacttg
     7141 gcacacattc aaccggatgt gtgcattttt ttatctccgc tttttttgaa atttaattac
     7201 ttttattgtt tgaaattcca ttctttaaga aatttaacaa gaaaaacgct tgctttttga
     7261 ccggaaaagc tgcataattc gttccgttag ctggttcgag tagtcagtta atagtttctc
     7321 ctctatttct cctttgtaga cttggcacac attctattga atgtgtgcat ttttttatct
     7381 gaagcaacaa gcctctgtgc gtgatgttgt tatgtttcat ttaggtgtca aaccgcatag
     7441 ccagcctgaa atattcaatc caaatccaaa accggatttt ctttgacctc ctccatcaca
     7501 acataactcc tactctccga agcggcaggc agttgcaata ggatattgcc tagcatatcc
     7561 cgataggcag acatatcggg caaacgtact ttaatcagat agtcgtattc gcccgacacc
     7621 aagtggcatt ccataatttg cggaattttc agcacttctt ttttgaaatc ttcgaaaata
     7681 ttgcccgatt tggattgcag cttcagctcg acaaaaacca atagtggttt gcccaacaga
     7741 tggggattga gatgggcgtg atagccggaa atataatgtt cccgctccaa acggcgcacc
     7801 ctctctgtaa cgggcgtggt ggacaagcct accttctcgg caagctccgt catcgggatg
     7861 cgggcattct gttggaggat cttaagaatg cggaaatcga ttttatctag ttctttcatt
     7921 tggattacct tgtatttatt attgatttta acaaatagag tatatagtgg attaacaaaa
     7981 accagtacgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct
     8041 tgtcctgatt tttgttaatc cactatatat ttgggaaagc gattatatca ggaaaagcaa
     8101 accgccttcc tacctgaaaa ctgctgcttc ggcttgaaga cacaagggtc tttaatattt
     8161 taaaagcctt gccgttggat tataattccc taattgattt cttaattttg ctaataaaca
     8221 cttgcttggt aaggaatgaa tttatgcgcc ctttgaacgt gcagatcagg ttgggcaacc
     8281 ttaggcacaa ttatcggatt ttaaaggaaa tgcacggagg caagctgttg gcggtagtga
     8341 aggccgacgc atacggacac ggtgcggtca gatgtgcttt cgcgctggca gacttggcag
     8401 acggctttgc cgtggcgaca atcgatgaag gaatcaggct gcgggagagc ggcattaccc
     8461 atccgattgt ccttttggaa ggcgtatttg aagcatcaga gtacgaagcg gtcgaacaat
     8521 actcgctctg gccggcagtc ggaaaccaat ggcaacttga ggctttgctt tcccgccatt
     8581 ggaaaaaacc tgtcaaggtc tggttgaaaa tggattcggg aatgcaccgt accggatttt
     8641 tccctcatga ttacgcttcg gcatatgcgg cattgaaaca gtcggaatat gtggacagta
     8701 ttgtcaaatt ctcgcatttc tcctgtgcgg acgaacccga aagcggtatg acggaaatac
     8761 agatggaagc attcgatttg ggtacggaag ggctggaagg cgaagaaagc cttgccaatt
     8821 cggcggctat tttgaatgtt cccgaagcac gcagggattg ggggcgcgcg ggcttggcgt
     8881 tatacggcat ttccccgttc ggaggcagcg atgacaggct gaaacccgtg atgaggcttt
     8941 caacccgtat ttttggcgaa cgcgttttac agccgcactc ccctatcggt tatggcgcaa
     9001 cattttatac cagcaaatct acgcgcgtcg gtctgattgc ctgcggttat gcggacggtt
     9061 atccgcgccg cgctccaagc aattcccccg tcgccgtcga cggtaaattg acccgggtca
     9121 tcggcagggt ctctatggat atgatgacca tcgagctgga cgcttcgcaa gaaggtttgg
     9181 gacacgaggt cgaactgtgg ggcgatacgg tcaacatcaa tactgttgcc gaagcggccg
     9241 gaaccatccc ttacgaattg atgtgcaata tcaaacgtgc aaaattcact tatatcgagt
     9301 aatcgagtcc aaacaaaaat gccgtctgaa gcctttcaga cggcatttcc ccatcaaaac
     9361 cgcaatcagt ttttcattga ttgaaccgga gccgggattc tgccgccccg tttgacgaac
     9421 acttcgcacg aaccttcttt gaccggcatt acaggcgcgt agcctaacag gccgccgaac
     9481 tcgacgctgt cgccgacggt tttaccggtt accggaataa tgcgcacggc agtggttttg
     9541 ctgttgatca tgccgatggc ggcttcgtcg gcaatgatgc cggaaatggt gtgcgcgggt
     9601 gtgtcgccgg gaacggcaat catatccaag ccgaccgaac aaacggcggt catcgcttcg
     9661 agtttatcca acgtcagcac gcctgcttcg gcggcggcaa tcataccttc gtcttcggaa
     9721 acggggataa acgcgccact caaaccgcca accgcgctcg aagccatcat gccgcccttt
     9781 ttcacggcat cgttcagcaa tgccaaagct gctgttgtgc cgtgcgtacc gcagacgctc
     9841 aaacccattt cttcaagaat gcgcgccacc gagtcgccga cggcaggggt cggtgccagc
     9901 gacaagtcga gaataccaaa cgggatattc agcatttttg aggcttcgcg gccgatgagt
     9961 tcgcccacgc gggtaatttt gaaggcggtt ttcttcacaa cttcggcaac ttcggtcaat
    10021 gtcgttgcat ccgaattttc caacgcggct tttacgacac ctgggccgga tacgccgaca
    10081 ttaatcacag catccgcttc gcccgatccg tgaaacgcac ccgccataaa cggattgtct
    10141 tccaccgcat tgcagaacac gacgattttc gcgcagccga aaccttcggg cgtaatttca
    10201 gccgtgcgtt tgatggtttc gcctgccagc ttgaccgcat ccatattgat accggcgcgc
    10261 gtactgccga cattgatgga gctgcacacg atgtcggtgg tcttcatcgc ttcgggaatg
    10321 gaacggatta acacctcgtc tgaaggcgac atgccttttt gcaccaacgc ggaaaagccg
    10381 ccgataaagg acacgccgat ggctttggca gccttatcca aagtttgcgc cacgctgaca
    10441 taggaatcgg catgggtagc cgccgcgatt tgggcaatcg gcgtaacgga aatgcgctga
    10501 ttcacaatcg gcacgccgta tttggcagac agatattttg ccgtcgccac caagtctttg
    10561 ccgaccgtgg taattttgtt gtaaatgttt tggttcaaca cattgatatc gctgctgatg
    10621 cagtcgtgca gatcaatgcc gatggtaatg gtgcggacat caaaattctg gtcggcaacc
    10681 attttgacgg tttctaaaat ttcgccggat tggatactca tcacattcct ccgactcaaa
    10741 tgcggtgcat cgcttggaag atttcttcat tttgcatacg gatatcaagc gcgagttttt
    10801 tgctctcttc cgcaaacaaa tccaaaacct cctgccgaga tttggggcat ttcgaggtat
    10861 ccaccaagat aatcatggta aaaaaatcat ccatcagctg ttggctgata ttgaggatgt
    10921 tgattcggtt ttccgccaaa attttggaaa catcgtacac gatgccgacg cggtctttac
    10981 cgatgacggt gatgactgaa ttgttcacag gcttactcct tgcagatatc cgttaaagtc
    11041 cgaaattata ccaccgttgg attttgaaga aatattgtca acaatatata catacaaaat
    11101 gccgtctgaa actatttcag acagcatcaa gattcagggt tcgattaaat aaccatcctt
    11161 atcccactgg gttttcctga ccaacttgtc atcctgataa acagcttcac tctttttaga
    11221 accatcttca taccactcta agactactcc attacgttta tgatgatgga tagacagttc
    11281 cgagagtaat cgaccgcttt catcccatgt cagaatcttg gccggctcat cattgaccat
    11341 aaccatttcc gttttgatat tgccatcagc ataccattgt ttccatacgc cgtttgcctt
    11401 attttgttta aactggattt cgctttcctt gctgccatta cggtaatagc ggtaccccgt
    11461 accttcactc aaaccatttt tataaggcat aaccgcagat tttttaccgt tcggatacca
    11521 gttgacccac tcaccgtccg gcttaccctt gctgaagccc cccgccattt ttttctgacc
    11581 attaaaatgc cacaaaatca acataccgtt ttgcagggta ggcacaaaag atttgatttg
    11641 cgttgaagca acgatataag gttcggaata tttcttcatc gacggataat aaaaatcctg
    11701 cgcgtacgca ataccctccg ccacactata ttgcctgata taagcggcag aagacatcgt
    11761 cgccgtcagt ttcccgttct gattaaaata aacagaatag gtctgtgccg ataaagaagc
    11821 cgaaaaaccc aacaggacaa ttgaaaatac aatccgagat aattttttca ttgcaatagc
    11881 gatataaaaa caaggctgtg ttttagtaat ctgttgattt caattatttg caagggaaaa
    11941 gacaattatt ttccggttag gaataaacct attctactga atatattgaa gccaagtacg
    12001 cctatcaaca ctatattaaa acactgccaa aaacaattaa cttataaaca atatggtaag
    12061 gatttctctg ccaagcatca aacccgagac aacgtatcgt aaaaatgccg tctgaaaaca
    12121 aatcgtcttc agacggcatt tccccttcaa ctcactcttc acccaataac tgctcgcgcg
    12181 tcaagaggaa aacaaaaccg tcgcccccgc tggtttccaa ccaagtaaaa ggcaactccg
    12241 gatacgctgc ttccaataca tccctgttat gcccgatttc caccagcaat acacctttgg
    12301 gattcagaaa ctttgccgca ttcagaagaa tctgcctggt ggcatccaac ccgtccgccc
    12361 cgctgcccaa tgccaattcc ggttcgtgca aatactcttc aggcaataac tcaaccgatt
    12421 ccgcatccac ataaggcgga ttggaaacaa tcaaatcata agtgccttcc aatccttcaa
    12481 acaaatccgt atgaataagc cggatgcgtt cttccaaacc ataatcttcg acattaatcc
    12541 ctgccacttc caaagcatcc aagctcacat caaccgcatc aatttgggca tcaggataat
    12601 gatgcgccat ctgaatggca aggcaaccgc ttccggtgca aagatccaaa gcattatgca
    12661 ccaactcatc gtattctatc caaggacgaa gtccgtcacc caacaattca taaataaaag
    12721 aacgaggtat gattacgcgc tcatccacat agaaatcaaa ctctccctgc catgcctggt
    12781 gtgtcaaata agcggctgga atgtgttcga cagcacgacg ctcaataacc gtcagcactt
    12841 cctctttttc agcttccaag agttttgcat caagatatgg ggcaagcata tccaaaggca
    12901 aattcaaagt atgcagaatc aaataagctg cttcatcatg cgcattatcc gttccatgac
    12961 caaaaaagag ccctgcctca ttaaaacggc tgactgcaaa acgtaaaaca tcgcggatag
    13021 tcgtcaattc ttgtgctgcc tgattaaaca taatatgaac cattctgcgt atagatactt
    13081 ttaattataa cagaaacaac aagcaaacct tttcatatcg ccaaataacc acccaatcta
    13141 cccatacaac tacataaatg cccgcgcgaa aaccatcgcc cgaacggaaa cgacaatggc
    13201 cgacggtatg ggcaacctga ttggctggga aaaaacgggg cttgttgccg gtaagcagtg
    13261 gataaccgca aaagacgaca aggtgtccga tgtctgcaat gccaacggcg agatgggcgt
    13321 aatcgggctt tacgagcctt tctcacacgg cgcattgacg atacccggtc atccgaactg
    13381 ccgatgcgag gttgtttccg tatcgggtgg cgaattgggg gaatttgccg aaaaaaagga
    13441 gcttcgtaaa gcggctatgc agtatgcgcg ggataacttt atcggcaaaa gctatgtcaa
    13501 tgaaaacagc gggcatgaac tgaaggtaac ttggcaaggt gtgaaacacg ctgcgtcaaa
    13561 ggcaaatcag gcggaattat ccatcatgac aaaacttgat gacttattgc gctacgcaaa
    13621 atatgagggt tcttattcgg ataggaaagg tcatcctaat attattgcag cacataagta
    13681 tcgtgccgtt gccaaggttg ggaatgagtc tttaaatatc ggtgtgattg taagggaatt
    13741 tccagacgac cataaacatt acgaccattt catcttgaag gatgaataaa gcccttttgc
    13801 agtgtcgttc tggagcggat agcgttaagg caagtacact tccagccttg aaaaagggct
    13861 ttaaattcag catgccattt atacaggcag gagtaaaccc atgacaaagt tatacgcaga
    13921 aatcgccaag atggagacgc aggacgacga cacggtcaag gtttggggtt acgcttcaag
    13981 cgaggaaatc gattcggacg gcgaagtcat cgcgacggca gctatgaagg cggcgattcc
    14041 cgattatatg aagtttggcg aggggcgcga gatgcacggc tcaaacgctg cgggaacggc
    14101 aattgaaatc aacgttgaag acgacggcat aacctttttc ggtgctcata tcatcgaccc
    14161 cgttgtcgtg gcgaaagtca aaacaggcgt ttacaaaggc ttttccatcg gcggcagcgt
    14221 taccgcccgc gatgatttga acaagtccca aatcacgggc ttgaagctga cggaaatcag
    14281 ccttgtcgac cgccctgcca atcccgatgc ggtgtttacc tgctttaagg cggacaaagg
    14341 tgcggaagcg gtaaacaacg atacagaaca taatgctaca tattttagcc atttcccttc
    14401 caaacaaaaa agcaccggcg gcagccgatg ccccttcctt tacaggttcc
//
LOCUS       DAWHXS010000053        14175 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWHXS010000053 DAWHXS010000000
VERSION     DAWHXS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.053"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            235..1194
                     /locus_tag="WIE22_001208"
     CDS             235..1194
                     /locus_tag="WIE22_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127293.1"
                     /translation="MYQNYDIVILSLGNGLLTRFFRNSSKLNLTLPIVITLFPGVIFG
                     DSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPILRKKHHCSDGNKV
                     YFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDITVHEDKYSYAELA
                     KNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGVISDLGVDKKYAN
                     THFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLLEVTSTQPPSFDN
                     YFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1242..2105
                     /gene="galU"
                     /locus_tag="WIE22_001209"
     CDS             1242..2105
                     /gene="galU"
                     /locus_tag="WIE22_001209"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ4127294.1"
                     /translation="MQKIRKAVFPVAGMGTRFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEATVAYGLKHPELK
                     DDFKELLKQFV"
     gene            2188..3204
                     /gene="galE"
                     /locus_tag="WIE22_001210"
     CDS             2188..3204
                     /gene="galE"
                     /locus_tag="WIE22_001210"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ4127295.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNHPN
                     GYDD"
     gene            3300..4361
                     /gene="rffG"
                     /locus_tag="WIE22_001211"
     CDS             3300..4361
                     /gene="rffG"
                     /locus_tag="WIE22_001211"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ4127296.1"
                     /translation="MKKILVTGGAGFIGSAVVRHIINDTQDAVVNVDKLTYAGNLESL
                     ESLANHPRYAFEQVDICDRAALDRVFAEHQPDAVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWHDMPSEKKAAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLNN
                     QTWWSRVLDGSYNRERLGS"
     gene            4574..5449
                     /gene="rfbA"
                     /locus_tag="WIE22_001212"
     CDS             4574..5449
                     /gene="rfbA"
                     /locus_tag="WIE22_001212"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ4127297.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            5504..6043
                     /gene="rfbC"
                     /locus_tag="WIE22_001213"
     CDS             5504..6043
                     /gene="rfbC"
                     /locus_tag="WIE22_001213"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ4127298.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            6205..8253
                     /locus_tag="WIE22_001214"
     CDS             6205..8253
                     /locus_tag="WIE22_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ4127299.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGIYFNAEAPSRLEHILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYH
                     NLDLLRTVRERHPDAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            8390..9649
                     /locus_tag="WIE22_001215"
     CDS             8390..9649
                     /locus_tag="WIE22_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ4127300.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLASFAAHAP
                     ADTNLIIKHHPMDRGFIDYSRDIKRFIKKHPKLKGRITYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAHYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSDSSTPAT"
     gene            9825..11231
                     /locus_tag="WIE22_001216"
     CDS             9825..11231
                     /locus_tag="WIE22_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ4127301.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELVAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            11449..12663
                     /gene="gltS"
                     /locus_tag="WIE22_001217"
     CDS             11449..12663
                     /gene="gltS"
                     /locus_tag="WIE22_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ4127302.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            12762..13778
                     /locus_tag="WIE22_001218"
     CDS             12762..13778
                     /locus_tag="WIE22_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ4127303.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            13789..13986
                     /locus_tag="WIE22_001219"
     CDS             13789..13986
                     /locus_tag="WIE22_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127304.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            14004..14153
                     /locus_tag="WIE22_001220"
     CDS             14004..14153
                     /locus_tag="WIE22_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127305.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFLCVSYADVPTHRTIQKAS
                     PYVPS"
BASE COUNT         3735 a         3590 c         3284 g         3566 t
ORIGIN      
        1 ttaatatcta atatttaaaa tacaatgaat aaaaaacgag tcctattaat tgctagttat
       61 gattcttttt tgaatactgg atatgctgtt gcaaaagaaa ttaactattc aacaatcgat
      121 atttatatcc accaaacaaa aaaaaatatt ctttcggaat cacaattaag tttaaatggg
      181 ctttctttaa aagaaatcaa tttttttcat attgatgatt atttcgacaa attaatgtac
      241 cagaattatg atattgttat tctatcatta ggtaatggtt tactgacgcg tttttttcgg
      301 aatagtagca aacttaattt aacattacct atagtaatta ctctatttcc aggggtaatt
      361 tttggtgatt cagaaagtat tataactagg ttaggtgcta atattttatt gttcaataat
      421 tattatgatt accaaatagg caataagttc aaagaacgtt acaacttatc gtgtacaaat
      481 attttatatg gttatcctat cctacgaaaa aaacaccatt gctctgatgg caataaagtt
      541 tatttcattg atcaagtaaa aattccagat ggaaaaaaag aaagattatt aattctaaaa
      601 aaattaatag cactggctaa tcgctatcca cataaagatt tagtactatt attacgtgtc
      661 catcaaaatg atatcacagt acatgaagat aaatactctt atgctgaatt agcaaaaaat
      721 atgaaattgc ctgataattt taaaatagag agaaaacgga cacaagatgc cttacaagaa
      781 atgggatatt gtttatcatt ctcttcaaca atgttatttg aagctgaaag ctgcggaatt
      841 cctattggtg tcattagtga tttaggggtt gacaagaaat atgccaatac acatttcaaa
      901 gatagtggtg ttttggttaa tttagatacg gtagacttgg actctccaaa taccattaat
      961 ccaacttggg ctgaactata tactaatcca gatagcctct cttcaaaaga gtttaaccaa
     1021 ttattagagg ttacttctac tcaaccacct agttttgata attattttat tgcagttaat
     1081 gacgttcagc ctgtatctat ggttctgtta cgtaaattta aaaagcttat atatcatcct
     1141 aagcaattct ttgttgattc aaaactatat aaattaatta aatttactaa ataattttta
     1201 ctatgggttg ccttatttat ttcaaaggag tgacataaaa aatgcaaaaa attcgtaaag
     1261 ctgtattccc tgtagctggc atgggaaccc gattcttacc tgccacgaaa gccagcgcta
     1321 aagaaatgct gccaatcgtg gacaaaccgt taattcaata tgctgcggaa gaagccgttg
     1381 ccgcgggttg tactgaattg atttttatca ccggccgtaa taagcgtaat attgaagacc
     1441 acttcgataa agcctatgag ttggaaactg aactggaaat gcgtgaaaag cataaattgc
     1501 ttggctatgt gcaaaaaatt ctgcctgatc atgtcagctg tattttcatt cgtcaggcag
     1561 aagctttggg cttgggtcat gcggtattat gcgcccgtcc tgctgtcggt aatgagcctt
     1621 ttgccgtgat tttggccgat gacttgattg atgcaccaca aggcgcactc aagcagatgg
     1681 tagatattta caatcaaagc ggcaacagca ttttaggggt agaaacagtc gatccttcgc
     1741 aaaccggttc atacggcatt gtcgaagtgg aacagctgaa aagctatcag cgtgtaacca
     1801 atattgtgga aaaaccaaag cctgaagatg cgccgtcaaa tttagcggta gtcggccgtt
     1861 atattttgac accgcgtatt tttgatttgc tgactaatct acctcgtggt gcaggtggtg
     1921 aaatccagct aactgatggt attgcccgcc tacttgacca tgaatttgtc ttggcgcatg
     1981 cgtttgaagg taagcgttat gattgtggca gtaagttggg ctatctggaa gccacggtag
     2041 cttatggctt gaagcatccc gaattaaaag atgatttcaa agaattattg aagcaatttg
     2101 tataatttta aggccgtctg aaaaagaaaa ggcatcatgt tttttacact ttcaggcggc
     2161 cttttaaaca taaagaagga aaacgtgatg aacattctcg ttaccggcgg caccggtttt
     2221 atcggctcgc acaccgttgt ttctttgctg aaaagcggtc atcaagtcgt gattttggat
     2281 aacctatgca attccagcat caatatcctg ccacgcttga aaacgattac cggccaagag
     2341 attccgtttt atcaaggcga tatccgcgat cgtgagattt tgcgccgtat ttttgcggaa
     2401 aaccgcattg attcggtgat tcattttgcc ggcttgaaag cggtgggtga aagtgtggcc
     2461 gagccgataa aatattatga taataatgtt tccggcagct tggtgttggc ggaagaaatg
     2521 gcgcgtgcgg gcgtgtttag cattgtgttc agttcttcgg cgacggttta tggcgatccg
     2581 ggcaaagtgc cttataccga ggatatgcca ccgggcgaca ccaccagccc ttacggcgca
     2641 tcgaaatcga tggttgagcg cattctcact gacattcaaa aagccgatcc gcgctggagc
     2701 atgattttgt tgcgttattt caatccgatt ggcgcgcatg aaagcggttt gattggcgag
     2761 cagccaaacg gtatcccaaa taatttgttg ccttatatct gccaagtggc ggcaggcaaa
     2821 ctgccgcaat tagcggtatt cggtgatgac taccctaccc ctgatggcac gggtatgcgc
     2881 gactatattc atgtgatgga tttggcagaa ggccatgtcg cggctatgca ggcgaaaagc
     2941 aatgtagcag gcacgcattt gttgaactta ggttccggcc gcgcttcttc ggtattggaa
     3001 atcatccgcg catttgaagc ggcttcgggt ttaaccattc catttgaaat caaaccgcgc
     3061 cgcgccggtg atttggcgtg cttctatgcc gacccttctt atacgaaggc gcaaatcggc
     3121 tggcaaacgc agcgtgattt aacccaaatg atggaagatt catggcgctg ggtaagtaat
     3181 catccgaatg gttatgatga ttaagtcatg aaattgagag gccgtctgaa gtttcagacg
     3241 gcctttgttt gcgatttata gtaaaattta ccttatcatt ttggcgatta atattcatca
     3301 tgaaaaaaat ccttgtcacc ggcggtgccg gttttatcgg ttcggcggtt gtacgccata
     3361 tcatcaacga tacgcaagat gcggtggtga atgtggataa gctcacgtat gcgggcaatt
     3421 tggaatcgct ggaatctttg gccaatcatc cgcgctatgc gtttgagcaa gtcgatattt
     3481 gcgaccgcgc ggcactggat cgtgtgtttg ccgaacacca gccggatgcg gtgatgcact
     3541 tggcagcaga aagccatgtt gaccgcagta ttgatggccc agcggcattt attgaaacca
     3601 atattgttgg tacttacacg cttttggaag cggcgcgcgc gtattggcat gatatgccgt
     3661 ctgaaaaaaa agcggctttc cgtttccatc atatttcgac tgatgaagtg tatggcgatt
     3721 tgcacggcac ggatgatttg tttaccgaaa ccacgcctta cgccccttcc agtccttatt
     3781 cggcttccaa agcctccagc gaccatttgg tgcgcgcatg gctgcgtact tacggcctgc
     3841 cgaccattgt gaccaattgt tctaataact acggccctta tcattttcct gaaaaactga
     3901 ttccactgat gattctgaat gcgcttgatg gcaaaccgct gccggtgtat ggcgacggta
     3961 tgcagattcg tgattggctg tttgttgaag atcatgcgcg tgcgctgtat caagtggtca
     4021 cagaaggcga aatcggcgaa acttacaaca ttggtggcca caatgaaaaa gccaatattg
     4081 aagtggtgcg caccatttgc gccctgctgg aagaattggt gccgaacaaa ccgcaaggcg
     4141 tggcgcgtta tgaagatttg attacctatg tcaaagaccg ccccggccat gatgtgcgct
     4201 atgcgattga tgccgcaaaa atcggccgtg agttgggttg gcagccgcaa gaaacttttg
     4261 aaagcggcat ccgcaaaaca gtggaatggt atttaaacaa tcaaacttgg tggtcgcgcg
     4321 tgttagacgg cagctacaat cgtgaacgct taggcagtta aatagagacc tttgcaaact
     4381 ccccatcttt ggcacatttc ttcgttatgc gctgctcgaa agcttgccta tctttgtgat
     4441 atgtctgcgc tttctgcgct gctataactt cgaactgtac tcaaatctgt ggctttgcaa
     4501 aggcctcaaa taataaaaat acgttcagac ggcatgaagg ccgcctgaaa aactacataa
     4561 ggaaagtaaa ctgatgaaag gcattattct cgcaggcggc agcggtatcc gcctttaccc
     4621 gattacccgc ggcgtatcaa agcaattact accggtgtac aacaaaccga tgatttatta
     4681 ccccttgtcg gtgttgatgt tggccggaat ccgcgatgtg ctggtgatta ccacccccga
     4741 agacaacgat gctttccgcc gcttactggg tgatggcagc gatttcggta ttcaaatttc
     4801 ctacgccatc caacccagcc ccgatggttt ggcgcaagcc tttttaattg gtgaagaatt
     4861 tatcggcgat gattcggtgt gtttggtgtt gggcgacaat attttctatg gccagcattt
     4921 cagccaatcc ctgaaagaag ccgccgcttc cgtggaaacc aaaggtgcta ccgtattcgg
     4981 ctatcaagtg aaagatcccg agcgtttcgg tgtgattgag tttgatgaga atttcaaagc
     5041 cctgtcaatc gaagaaaaac cgaaacaacc taaatccgat tgggcggtaa ccggcctcta
     5101 tttctacgat aatcgcgtag tcgagtttgc caaacaagtg aagccgtctg aacgcggcga
     5161 attggaaatt accaccttga atcaaatgta tttggacgat ggttcgctca atgtccaatt
     5221 gctcggtcgc ggtttcgcat ggcttgatac cggcacacac gccagcctgt tggaagcagc
     5281 ctcgtttgtc gaaaccattg aaaacattca aaacctacaa gtcgcctgtt tggaagaaat
     5341 cgcatggcgt aatggctggt tgagcagcga agaaatcaga aacttggcaa agccaatgtg
     5401 taaaaatgaa tatggccgct atttattgcg cttggttggc aaagattaag aggttgcttt
     5461 taccagtttt caggccgtct gaaacgcttt ttaggaaaac atgatgaata tcatcgatac
     5521 ccgcattccc gatgtcaaat tgctcgaacc caaagtcttc ggtgacgagc gtggtttttt
     5581 tatggaaacc ttccgtgatg aatggttccg ccaaaatgta gccgagcgca ctttcgtgca
     5641 ggaaaaccac agcaaatcca cccaaggcgt attacgcggt ttacattatc aaaccgaaaa
     5701 tacacaaggc aaactggtgc gcgtggtatc aggcgaagtc tttgatgttg ccgtggatat
     5761 gcgtcgtgat agtcctactt tcggccactg ggtcggtgag gttttatcgg cagacaacaa
     5821 acgccaatta tgggtgccgg aagggtttgc ccatggattt tatgtgttga gcgatgaagc
     5881 ggaattcgtc tacaaatgca ccgactatta caacccgaat gcggaacatt ccctaatttg
     5941 gaatgatgcc acagtgggaa tcgactggcc tttattggga gaaccaagcc tatcggccaa
     6001 ggatttggca ggcaagatgc ttgctgaagc ggccacattt taattttcac ttttaggtca
     6061 tcgtgggtaa tttgtagcaa gatgacctaa tttttaccag ctagcctact aactaaattt
     6121 ctactatatt taaatagtca gaaaatttac acaaaatctg ttaataagat attatattgt
     6181 cagtaatatt ttttcctatt tattgtgatt cctgcctaca ttcctcttcg ctctttcggt
     6241 atacgtaaaa ttccgaatat tacgcaattt ctaccggaat attcacttaa caaaaccgta
     6301 ttcaacgccc aaaccatcat tggctggggc ttacgcccga ccacccacca agcacgtgct
     6361 tttgcacaat cacatggctt gccgtttgta gccttggaag acggtttttt acgctcactg
     6421 ggcttaggcg tggccggtta tccgcccttc gcactggttt acgacgatat cggcatctat
     6481 tacgacacaa cccgcccatc gcgtttggag cagttgattt tagccgcaaa catgccgtct
     6541 gaaatgctta accaagcgca gcaggccatt gatttgattt tgacgcaccg tttgtctaaa
     6601 tataaccatg ctctggattt ttcagacggc ctcgtacaag acagcaaaaa cattgttttg
     6661 gtaattgatc aaactttcgg cgatatggcg attcaatatg gcggcgcaga tgaagccaca
     6721 ttccgccaaa tgtttcagac ggccttagcc gaaaatcctg atgcggaaat ctgggtcaaa
     6781 acccatcccg atgtattgag cggcaagaaa cagggttacc ttactgattt ggcacagcag
     6841 ccgcgtgtgc gtttattggc tgaagacatt aatccgattt cgctgctgca agcggtggac
     6901 aaagtgtatt gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg
     6961 gtaacgtttg gtttgccgtg gtatgccggc tggggtgtaa gcgacgaccg ccatcctgaa
     7021 atcaaccgcc ttgttcaaac ccaacgccgc gccacccgca acttgctgca gctcttcgcc
     7081 gcagcctatc tgcaatacag ccgctacctc aaccccaata ccggcgaagc aggcagcctc
     7141 tttgatgtca tcgactatct ggcgacggtc aaacgtaaaa acgacaaatt gcgtggcgag
     7201 ttatattgcg tcggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta
     7261 ccctcttgcc gtctgaaatt tatctcttcc acccaaaaac tggcaagggt caaactgtcc
     7321 gacgatgcac gcatcctggc ttggggcaac ggcaaagagg ccatcgtccg ctttgccgaa
     7381 caacaccaca tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc
     7441 tccaacttag tgccgccgct gtcgctcgtt accgacgata tgggtattta cttcaatgcc
     7501 gaagccccat cccgccttga acacatcctg caaaaccaaa acttcgacga tcaagacttt
     7561 cagacggcct tgaagttgca aaaaatgctg accgaaaacc atatcagcaa atacaacgtc
     7621 ggcaactcag actttaccgc cccatcaacc gacaaaaccg tgatcctcgt tcccggtcag
     7681 gttgaggatg atgcgtctat ccgctatggt tcgccccaaa tctatcacaa tctggatttg
     7741 ctccgtaccg tacgcgaacg ccatcccgat gcctatatca tctacaaacc gcatcccgat
     7801 gtagtcagcg gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac
     7861 caaaccgccg aacaagccga catcctgacc tgtctccaat acgcagacga aatacatacc
     7921 atgacttcgc tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc
     7981 ctgccttttt acgcaggctg ggggcttacc caagatctgc tccccatccc gcgccgtagc
     8041 cgcagacttg agctttggca gctgattgcc ggcacgctca tccactatcc cgactacatc
     8101 caccccgaaa cccatcaggc catcaatgcg gaaaccgctg cccaaatcct gatacgacaa
     8161 aaaaatatgc aaaaaaacaa caacggatta catcgcgggt gctttgccaa aaaattaggt
     8221 aaaatcaaac aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat
     8281 aaacttgcct ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt
     8341 gaaagttcaa cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac
     8401 cgtcctcaaa aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca
     8461 aggccctgtc ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac
     8521 cgtacataaa ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca
     8581 taccgttgtt tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac
     8641 tcaacatcac atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc
     8701 aaaacgcatt gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg
     8761 cccctactac atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccgcgccg
     8821 tgccgacttt tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac
     8881 gccggtacac ggcggtttta cgcccatggc aaaaaacgct atccgttact atatcgagtt
     8941 gttccgcaat ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca
     9001 ttacctcaaa ccatggtcgc tctccatcct caagcgtttg aactactata ttgaagacat
     9061 ccaaatcgcc aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca
     9121 ggtattcaac gacagccaag tccgtatcca ttgcgacttt cccagcgtcc gcagcttcct
     9181 gctccatgtt ttggcctcct ttgccgcgca cgcgcctgcc gataccaacc tcatcatcaa
     9241 gcatcatccg atggaccgcg gctttatcga ctactcgcgc gacatcaaac gctttatcaa
     9301 aaaacacccc aaactcaaag gccgtatcac ttacgtccat gatgtccccc tgcccgtttt
     9361 cctgcgccac ggcctcggca tggttaccat caacagcacc agcggcctgt ccggcttgat
     9421 ccacaacatg ccagtcaaag tcctcggccg cgcccattac gacattcccg gcataaccga
     9481 ccaaaatacc ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgttcca
     9541 tgcctaccga atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt
     9601 cttttttcct aacaaaaaaa cctccgactc ttccactcca gcaacctgac ttagcaaaaa
     9661 taattcaggc cgtctgaaaa catttcagac ggcctgaaac aatcaatacc ttagctactg
     9721 ccatgtaaat aaaacacaaa aacctatttt tacaaagccc tcaaccggta tcgccgccca
     9781 tatgccgcag catccgtctt ccactttata tccgcccgca aaccatgacc gccgctcctg
     9841 atatcctcta ccggcaagcc gccgcccttt tggaacaatc caataccgcc caagccctgc
     9901 ccctgttgca acaggcggca gagcaaggtt atgcggaagc tgctttcgta ttgggcaacc
     9961 atctgctgca aaacggccaa ccggagcagg cactttcatg gttggaagcc gccgcggccc
    10021 aacgccatcc caaagcactc ttctccctgc tgcaacaacg cgaacacaac ggcaccccga
    10081 ccggacagct tctcaacgac tatgcctggc tgggtgagca ggggcactca gaagcccaat
    10141 taatcctcat gcgttaccac gcgcaacgca acgatccaca atcgctctac tgggcggaac
    10201 ttgttgccgc ccgatatgcc gcacctgcgt attaccatct ggcacgccat catcaacgcc
    10261 aaggcgacgt tgaaacagcc atcgaacaat acgaaaaagc ggcagcactc ggcgtaactg
    10321 ccgcctgctg gcaacttggt caaatctact tctacggtac aggtgtcagc cccaaccacg
    10381 cacaagccga acactatctc gaacaagccg cgcaagccgg ccacatcgcc gcacaaaccc
    10441 tgctggccga ccttcttgct gcccaacgca aacccgaagc cttagaatgg tatcgtcgtg
    10501 ccgccgacaa agagcaagcg gaagcacagg ccaagctggc tcaatacgcc ctgaccggcg
    10561 aactttccga acgcgatccg tttcaagcgg cacgatatgc caaagccgcc gctgagaaag
    10621 accatcccga agccctgaaa atcatgggcg acctctaccg ctacggcctc ggcatcaaag
    10681 ctgacaacca tatcgcgcac gattactacc accgtgctgc cgcgctgggt tctgccgccg
    10741 cagcacaaaa actcatcagc gacgccgcgc tgtaccatcc gcaacaatac gaacaaatca
    10801 aaactgccgc cctgcaacag caacaaaccg aaaccatcta ccgtttggcg gaaacacaag
    10861 cccatgccat cggtcgtccc gccgactata atgccgcacg caaaaattac atggaagctg
    10921 ccgggttcca ccataaaaac gcggcggctg ccttaggccg catctaccat tacggcctcg
    10981 gtacggcgca agatcctcgg gcggctgcac actggtacgc cattgctgcc gaacaaaacc
    11041 acccttccgc ccaataccac ctcgcctgtt tttactatca cgggcaaggt gtcggctgtc
    11101 atgttccgac cgcctgctac tggctgcagg ccgccatcgg caacggccac acttcggccg
    11161 aatcattaat atccctatta gaacaatggc gacgcgaagc acaccatgcc atcggacaaa
    11221 aggccgtctg aaaagattta cactcgcatt ttttgacaat ctttaactat tcccctaata
    11281 tttgccagtt atttttcacg gacacgccat tgttttcatt tctttctgaa aacaccttgt
    11341 ccgcgcatca ataccatgac actcggcgga taacgccaag cgttgaaaca cactacatcc
    11401 ggaacaaaaa cggatgctcg gaaaaatatt tctaggaggt gaaacaacat ggaatgggaa
    11461 ttcaacagtt attacacact gattgccgcc acgctcgtgt tgctggttgg taaatttctg
    11521 gttcaaaaaa tcaaattctt acgagacttc aatattcccg agccggtcgc tggcggtttg
    11581 gttgccgcga ttatcctgtt tgcgctgcac gaggcgtacg gcgtgagctt caaatttgag
    11641 aaaccgctgc aaaatgcgtt tatgctgatt tttttcacgt ccatcggctt gagcgcggat
    11701 ttttcccgtt tgaaggcggg cggtttgccg ctggtggttt ttaccgcgat tgtgggcgga
    11761 tttatcttgg tgcaaaactt tgtcggggtc ggactggcta cggctttggg tttggacccg
    11821 ctcatcggtc tgattaccgg ttcggtgtcg ctgacgggcg gacacggtac gtcaggtgcg
    11881 tggggaccta attttgaaac gcaatatggc ttggtcggcg caaccggttt gggtattgca
    11941 tcggctactt tcgggctggt gttcggcggc ctgatcggcg ggccggttgc gcgccgcctg
    12001 atcaacaaaa tgggccgcaa accggttgaa aacaaaaaac aggatcagga cgacaacgcg
    12061 gacgacgtgt tcgagcaggc aaaacgcacc cgcctgatta cggcggaatc tgccgttgaa
    12121 acgcttgcca tgtttgccgc gtgtttggcg tttgccgaga ttatggacgg cttcgacaaa
    12181 gaatacctgt tcgacctgcc caaattcgtg tggtgtctgt ttggcggcgt ggtcatccgc
    12241 aacatcctca ctgccgcatt caaggtcaat atgttcgacc gcgccatcga tgtgttcggc
    12301 aatgcttcgc tttcgctttt cttggcaatg gcgttgctga atttgaaact gtgggagctg
    12361 accggtttgg cggggcctgt aaccgtgatt cttgccgtac aaaccgtggt gatggttttg
    12421 tacgcgactt ttgttaccta tgtctttatg gggcgcgatt atgatgcggc agtattggct
    12481 gccggccatt gcggtttcgg cttgggtgca acgccgacgg cggtggcaaa tatgcagtcc
    12541 gtcacgcata ctttcggcgc gtcgcataag gcgtttttga ttgtgcctat ggtcggcgcg
    12601 ttcttcgtcg atttgattaa tgccgcgatt ctcaccggtt ttgtgaattt ctttaaaggc
    12661 tgattttccg cctttccgcc aaagcacctg caaggtttac cgcctgcagg tgcttttgct
    12721 atgatagccg ctatcggtct gcaccgtttg gaaggaacat catgtatcgg aaactcattg
    12781 cgctgccgtt tgccctgctg cttgccgctt gcggcaggga agaaccgccc aaggcattgg
    12841 aatgcgccaa ccccgccgtg ttgcaaggca tacgcggcaa tattcaggaa acgctcacgc
    12901 aggaagcgcg ttctttcgcg cgcgaagacg gcaggcagtt tgtcgatgcc gacaaaatta
    12961 tcgccgccgc ctacggtttg gcgttttctt tggaacacgc ttcggaaacg caggaaggcg
    13021 ggcgcacgtt ctgtatcgcc gatttgaaca ttaccgtgcc gtctgaaacg cttgccgatg
    13081 ccaaggcaaa cagccccctg ctgtacgggg aaaccgcttt gtcggatatt gtgcggcaga
    13141 agacgggcgg caatgtcgag tttaaagacg gcgtattgac ggcagccgtc cgcttcctgc
    13201 ccgtcaaaga cggtcagacg gcatttgtcg acaacacggt cggtatggcg gcgcaaacgc
    13261 tgtctgccgc gctgctgcct tacggcgtga agagcatcgt gatgatagac ggcaaggcgg
    13321 tgaaaaaaga agacgcggtc aggattttga gcggaaaagc ccgtgaagaa gaaccgtcca
    13381 aacccacgcc cgaagacatt ttggaacaca atgccgccgg cggcgatgcg ggcgtacccc
    13441 aagccgcaga aggcgcgccc gaaccggaaa tcctgcatcc tgacgacggc gagcgtgccg
    13501 ataccgttac cgtatcacgg ggcgaagtgg aagaggcgcg cgtacaaaac cagcgtgcgg
    13561 aatccgaaat taccaaactt tggggaggac tcgataccga cgtgcaaaaa gagttggtcg
    13621 gcgaacaacg caagtgggcg caggaaaaaa tcagcaactg ccgacaagcc gccgcgcagg
    13681 cagaccggca ggaatacgcc gaatacctca agctgcaatg cgacacgcgg atgacgcgcg
    13741 aacggataca gtatcttcgc ggctattcca tcgattaggg gcaaaccgat gactaccgtc
    13801 cccaaaagca ggattcccgt caaaccgctg cccgaaaaaa ccacagacga agccaaagtc
    13861 gaaaaatggc ggcagctcgg tgcggaacac ggtttgtcgg gcgaatgggc agttgccgcc
    13921 agattgggcg agaacggttt taccgaagaa cagatggaaa atatcgccaa cctgttcggc
    13981 agataaaggg aaaattgacg gaaatgccgt ctgaaaccct gttatcggtt tcagacggca
    14041 ttttgactaa tacggtacgc aggcgcaaaa cagccggctt tttatgtgtt tcctatgctg
    14101 atgttccaac acacaggacg atacaaaaag cgtcgcccta tgtgccgtcc tgattcggaa
    14161 agggttacgc tcctc
//
LOCUS       DAWHXS010000054        14053 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWHXS010000054 DAWHXS010000000
VERSION     DAWHXS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.054"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..841)
                     /gene="mafA"
                     /locus_tag="WIE22_001221"
     CDS             complement(<1..841)
                     /gene="mafA"
                     /locus_tag="WIE22_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ4127306.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFS"
     gene            994..1098
                     /locus_tag="WIE22_001222"
                     /pseudo
     CDS             994..1098
                     /locus_tag="WIE22_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1105..1254)
                     /locus_tag="WIE22_001223"
                     /pseudo
     CDS             complement(1105..1254)
                     /locus_tag="WIE22_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1480..2199)
                     /locus_tag="WIE22_001224"
     CDS             complement(1480..2199)
                     /locus_tag="WIE22_001224"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ4127307.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2443..3297)
                     /locus_tag="WIE22_001225"
     CDS             complement(2443..3297)
                     /locus_tag="WIE22_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ4127308.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPATVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKKAVDYAA
                     EVAAAAKV"
     gene            complement(3426..4154)
                     /gene="rpsB"
                     /locus_tag="WIE22_001226"
     CDS             complement(3426..4154)
                     /gene="rpsB"
                     /locus_tag="WIE22_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ4127309.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4327..4560)
                     /locus_tag="WIE22_001227"
     CDS             complement(4327..4560)
                     /locus_tag="WIE22_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127310.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            4638..5219
                     /locus_tag="WIE22_001228"
     CDS             4638..5219
                     /locus_tag="WIE22_001228"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ4127311.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            5280..5870
                     /locus_tag="WIE22_001229"
     CDS             5280..5870
                     /locus_tag="WIE22_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4127312.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            6407..7873
                     /locus_tag="WIE22_001230"
     CDS             6407..7873
                     /locus_tag="WIE22_001230"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ4127313.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(8060..8434)
                     /locus_tag="WIE22_001231"
     CDS             complement(8060..8434)
                     /locus_tag="WIE22_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ4127314.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(8672..8971)
                     /locus_tag="WIE22_001232"
     CDS             complement(8672..8971)
                     /locus_tag="WIE22_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ4127315.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(9126..9905)
                     /gene="map"
                     /locus_tag="WIE22_001233"
     CDS             complement(9126..9905)
                     /gene="map"
                     /locus_tag="WIE22_001233"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ4127316.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(9945..10595)
                     /locus_tag="WIE22_001234"
     CDS             complement(9945..10595)
                     /locus_tag="WIE22_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127317.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            complement(10603..11211)
                     /locus_tag="WIE22_001235"
     CDS             complement(10603..11211)
                     /locus_tag="WIE22_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ4127318.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(11275..11868)
                     /locus_tag="WIE22_001236"
     CDS             complement(11275..11868)
                     /locus_tag="WIE22_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ4127319.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(11873..12220)
                     /locus_tag="WIE22_001237"
     CDS             complement(11873..12220)
                     /locus_tag="WIE22_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ4127320.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            12266..13141
                     /gene="rsmI"
                     /locus_tag="WIE22_001238"
     CDS             12266..13141
                     /gene="rsmI"
                     /locus_tag="WIE22_001238"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ4127321.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGQLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFPERRLMLAREITKTFETFLSGTVGEIQT
                     VLSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(13176..13580)
                     /locus_tag="WIE22_001239"
     CDS             complement(13176..13580)
                     /locus_tag="WIE22_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ4127322.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(13699..13809)
                     /locus_tag="WIE22_001240"
                     /pseudo
     CDS             complement(13699..13809)
                     /locus_tag="WIE22_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            13871..>14053
                     /locus_tag="WIE22_001241"
     CDS             13871..>14053
                     /locus_tag="WIE22_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4127323.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDG"
BASE COUNT         2975 a         3306 c         4063 g         3709 t
ORIGIN      
        1 cggagaaatc gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc
       61 ccatccacaa tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga
      121 tgagcaattt tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca
      181 gtgtttcggc attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga
      241 tgttaataaa cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga
      301 aaaatacggt ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg
      361 tttcatttcg ataatccccc atcccgccaa tatttaagcc cagactgctt ttacttccgc
      421 taccgtctga ttgggtgcga gagcgtgcag gggcattaag tgtagataaa gaagtggtta
      481 aacctgtcaa accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat
      541 cggtacggac ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc
      601 gaccccctgt caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt
      661 ttcgtccgtg taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca
      721 caagttcttg ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg
      781 tcccgcaggc ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca
      841 tagattttct cctttgatga aaaacaaatt gtatcaaaat tgtaaacaaa ttataaatta
      901 aataaaatat ttctttactg gttttgtggt ttggttttca ataggctgcc cgcacatagt
      961 gggatttacg gttgcaatgg ctgcggcgta ttttatagtg gattaaattt taatcaggac
     1021 aaggcgacga agccgcagac agtacaaata gtacggcaag gcgaggcaac gctgtactgg
     1081 tttaaattta atccactata ggcttttttc ggtcagttgc cggttaatca gtcccggcag
     1141 ttcggacagg gtgttgtctt gcgccaacct gttgcggtag cggcataagg tgctgtaatc
     1201 ggggatgctt ggtttgtcaa aacggcaaaa caggttgaaa tcggtgcggg tgatgggtac
     1261 ggcgttgccc ttatgccgat tttgtccgcc tgctgcaagg cggtttgtct aatgaaagga
     1321 aaaatgccgt ctgaacattg ttcagacggc attttcaaat ggaaggcaat atagtcggca
     1381 tatgaaaaat tgatgtataa ggcaggatgg gtttaggctt gttaatttcc atataataaa
     1441 tggccgtatg aagcccatat gcgactatat ctgccggaat cagcagtgaa ccagtgtacc
     1501 ctcgttttcg ccggttacca cgcgtttgag cgagccttct ttagcgatac cgaatactac
     1561 gatattgagc ttgcgttcgc ggcagagggc gaaagcggtg gcatccatca ctttaaggtt
     1621 tttcaacaag gcttcgtcaa aagtaatggt ttcgtagcgt gttgcagatg ggtctttttt
     1681 cgggtctgcg gtgtacacac cgtcaacgtt ggtggctttg agcatcacgt cgcagttcat
     1741 ttctgcaccg cgcaatgcgg cggcagtgtc ggtcgtgaag aacggattac cggtaccggc
     1801 ggcgaaaatc acgactttgc cttcttccaa atattgaatg gctttaggac gggcgtaagt
     1861 ttcggcgatt tgctgcatag acagtgcgga ttgtacgcgc gctttgatgc ccaaagtttc
     1921 aaatgcgtct ttgagtgcca acgcgttcat tacggttgcc atcatgccca tgtagtcggc
     1981 ggtggcgcgg tccatactgc ctgcttgtgc agatacgcca cggaaaatgt taccgccgcc
     2041 gacaacgata ccgacctgca cgcccatttt aacgacttcg gcaatttcgc cgacagtttg
     2101 aacgatggta tcgtgattga tgccgaacgg atcggaaccc atcagggatt cgccggagag
     2161 tttcagtaat acgcgtttgt atttgatttg ctgtgtcatg ggatattctg ctttctcgtt
     2221 ggggatgttg ccggcttccg ggcgggatgc cgtctgaaag gtgtatgcct tggcagggcg
     2281 tatgcggcgt gccggcggtg tgaaaacggg ctgctgaatt tgccgccgct gttttcgggc
     2341 ggggcgggca tttgaaccct gccgccggaa aatatgggcc tttcccaaaa aaaagcaccc
     2401 tgattcgttt ggaatccagg tgctttcttt ttcataagtg ccttacactt tggcggcagc
     2461 ggctacttct gccgcgtaat cgacggcttt tttctcgata ccgtcgccta ctttgtagcg
     2521 gacgaagctg accacttcag tgccgttttc tttagcgaat tgggcaacag tttggtcggg
     2581 gttcatcacg aacgcttggc cgttcagagt gatttcagcc aagaatttgc ggatacggcc
     2641 ttcaaccatt ttagcggcga tgtcggcagg tttgccggaa gcgatagctt gttcagtgta
     2701 gatgtggcgt tctttttcaa cggtttcagc atctacttcg gcttcactta cgcattgtgg
     2761 tttagcggca acgatgtgca taccgatttt gcgtgctacg tcttcagagc ctttgtactc
     2821 aaccaatacg ccttcggtag ccaaagcacc gtggatgtag gcaaccagtt ggttggcagt
     2881 gtcgatcact tggaagcggc gaacagacat gttctcgccc aatttagcga tgatggcttt
     2941 acgttctgct tcaaccagtt cgctcagttc ttcaacggta gccggttttt tctcggcagc
     3001 ggttttagca acgaagttgg caaattctac gaagcccgcg tctttggcaa cgaagtcggt
     3061 ttcgcagttt acttcgacca atgcgccgac attgccgttg atggcgtaag ctaatacgcc
     3121 ttcggcagcg gtacgaccgg ccagtttacc ggctttcgcg ccggatttga tgcgcaggat
     3181 ttcttcggct ttgtcgaagt tgccttcagc ttcaaccaag gcttttttgc attccatcat
     3241 gcccaagccg gtagcggcgc gcagatcggc aaccattttt gcagtaattt ctgccatttt
     3301 gaatctccta gatttttggg aaatacggaa aggattttcc gcattcggga atcgtgtgcc
     3361 gcgaacggac gttcagacgg catatttgag aaaaggggca taacgcccct cttctgtaca
     3421 ccgaattact cggcagcagc ttcttgggca gcggctacag tttcttgcag cgcttggttt
     3481 ttgccttcca aaactgcgtc ggcgatgccg cggcagtaca ggcggatggc tttggcggag
     3541 tcgtcgttgc cggggataac gtatttcacg ccgtcagggc tgttgttggt atcgactacg
     3601 gcgataacgg ggatgcccaa tttctcagct tcgaccagag tacctttttg gtagccggta
     3661 tcgataacga aaatcgcgtc aggcaggcct ttcatgtttt tgataccgcc caaagaacgt
     3721 tccagttttt caacgtcgcg ttgcatttcc agaatttctt ttttgttgaa accgccttca
     3781 gcagcgtttt ccagggcagc agttttttct tccaggcgtt tgatggattg cttaacggtt
     3841 ttgtagttgg tcagcatacc acccaaccag cggtaatcaa cgaaaggcat accggcacgg
     3901 gtagcttctt cgcggatgat gtcgcgggct tggcgtttgg tacctacgaa caatactgta
     3961 cctttgttgg caaccagacg acgtacggct tcttgcgcct cttggaacat cggcagggtt
     4021 ttttccaagt ttacgatatg gattttgttg cgcgcaccga aaatgtattg tgccattttc
     4081 gggttccaga aacgggtttg gtggccgaag tgaacaccgg cttcaatcat ctgacgcata
     4141 gtaatttgag acattttatt tccttgaaag ggttaagagc acacattcaa tcgcatagaa
     4201 cttgataggt cacgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa
     4261 aatagtgtgt ttccgaaatg gaaaactgac ggattatagc ggataagatg ccgtctgaaa
     4321 agggattcag ggttttgtgc cgctgtcttt ttgccgttgt gcgcgctgct gttcggattc
     4381 cgcctgttcc tgctgcctca tcaggtggtt gcggcggcgg acggtaatgg tgaaaaccgt
     4441 gaacacggtg ggcagcaccg cccaaaaaac caaataaatc aaggcgcgcg caatactcgg
     4501 ctgcgcggcg gaatacataa cggcaacaaa aagatagccg atggcgacga tgtgccacat
     4561 tgtgtgcgtc ctgtttgaat gttaacaaaa tcgttataat cgcaacattc taccgcaatc
     4621 ctgaaaggcc gtctgaaatg aggaacgagg aaaaacgcgc cctgcgccgc gaattgcgcg
     4681 ggcggcgttc gcaaatgggg cgggacgtgc gggcggcggc aacggtaaaa atcaaccacc
     4741 tgctcaaacg ttatattaaa aaagggcgga aaatcggcgt gtattggccg atgggcaagg
     4801 aattgcgttt ggacggcttt gtccgcgcgg cgcaaaaacg cggtgtaaaa ctttatctgc
     4861 cttatatcga accgcgttcg cggcggatgt ggtttacgcc gtatcctgaa agcggaatgg
     4921 aacgggagcg catacggggc agggcgaagt tgaacgtgcc gcagtttgca gggcgcaaaa
     4981 tccgcgtgca cggtttgtcg gtattgctcg tcccgcttgt cggcatagac cgcgaaggct
     5041 accgcttggg gcaggcaggc ggctattacg atgcgacgct tgcagcgatg aaataccgtt
     5101 tgcaggcaaa aaccgtgggc gtgggctttg cctgccaact ggtcggcacg ctgccgcgcg
     5161 aggcgcacga cctgccgctg gacggttttg tgtcggaggc ggggatattg tgtttttagc
     5221 cgttcagacg gcatttcggg cggaaatgcc gtctgaaggc ggaatattgg gagaaaagga
     5281 tgtcggaaaa gataatactg ccggtcttgt cattgggcgg cgttcggctc gaaccgttgg
     5341 atgtgcatca tgaaacgggt ttgcgcgagg cggtttgcga tggggaggtt tggaagctgg
     5401 gggtaacgtc cgcgccccat ccggataggg tggcagacta tatcgggaca gcattggcaa
     5461 cgcgtttggc gtttgcggtt gtcgatgaag aggcgggcag ggtggtcggg acaacggcgt
     5521 attatcattt tgaaccgcag attccgcgtt tggatatcgg attcacatgg tatgcggcat
     5581 cggcacggcg gacacggata aacacctgtt gcaaaatcat gctgttggat tacgcatttg
     5641 atgttttggc ttgccgttgc gtgggatggc gcacggatat tctgaacctt gcctcgcaac
     5701 gtgccatcga acggctgggt gcggaaaaag acggcgtgct gcgtatgcat atgctccgta
     5761 aagacggcag tgtgcgcgat acggtcgtgt acagtatgct gagggaagat tggtgcaaaa
     5821 acagggaaat cctgaccggc aggttggcag ggtatggcgt gcaggtgtga ggcagtattt
     5881 tcaaatttat agtggattaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag
     5941 aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt tgtactgtct
     6001 gcggcttcgt cgccttgtcc tgatttttgt taatccacta taaaaaactt aacgcaatat
     6061 gctgacaatc ttttaaaaat taatatataa acaattttga ttttttatct attaaaaatt
     6121 tgatatatat agataaataa ctatttttat ttttcatttc gcagtttccc gacttggcgg
     6181 gcgacagaaa aactttgcat ttcccagtaa aatccactac attcacgata acatattaca
     6241 agcccctgaa gcggtgtcgg aagccatgct tgcaacgcat tccaaccccg aaacagcata
     6301 ctcggacaca caccattgcc ggttttccct gcttttgcga ttcaggcgtt tgtagtacgg
     6361 caaataacca taagggcaga agatgcctgt tttttaggag taaaccatgg ctgaagcgac
     6421 agacgttgtc ttggtgggcg gcggcattat gagcgcgact ttgggcgttt tgctcaaaga
     6481 actcgaaccg tcttgggaaa tcaccctgat tgaacgcttg gaagatgtgg cgttggaatc
     6541 gtcaaacgcg tggaacaacg ccggcacggg gcattccgcg ctgtgcgaat tgaactatgc
     6601 gccgttgggt gcaaatggga ttatcgatcc ggcgcgcgcc ctcaatattg ccgaacagtt
     6661 tcatgtcagc cgccagtttt gggcgacgct ggtcgcggaa ggcaagttgg aagacaattc
     6721 cttcatcaat gccgtgccgc atatgtcttt ggtgatgaat gaagaccatt gttcttatct
     6781 tcaaaaacgt tatgacgcgt ttaaaaccca aaaacttttt gaaaatatgg aattttccac
     6841 cgatcggaac aaaatttccg attgggctcc gctgatgatg cgcggccggg acgaaaacca
     6901 acccgtcgcc gccaactact ccgccgaagg cacggatgtc gatttcggac ggctgacgcg
     6961 ccaaatggtg aaatatttgc agggcaaggg cgtaaaaacc gagttcaacc gccacgtcga
     7021 agacatcaaa cgcgaatccg acggcgcgtg ggtgctcaaa accgccgata cccgcaaccc
     7081 cgacgggcag ctcaccctcc gtacccgctt cctcttcctc ggcgcgggcg gcggcgcgct
     7141 gaccctgctg caaaaatccg gcatccccga aggcaaaggc tacggcggct tccccgtgtc
     7201 cggcctgttc ttccgcaaca gcaaccccga aaccgccgaa caacacaacg ccaaagtgta
     7261 cgggcaggct tccgtcggcg cgccgccgat gtccgtcccg cacctcgaca cacgcaacgt
     7321 ggacggcaaa cgccacctta tgttcggccc ttacgcaggc ttccgttcca acttcctcaa
     7381 gcaaggctcg cttatggatt tgccgctgtc catccatatg gacaatctct atcctatgct
     7441 gcgcgccggc tgggcgaata tgccgctgac caaatacctg ctgggcgaat tgcgtaaaac
     7501 caaagaagaa cgcttcgcct ccctgctgga atactacccc gaggcaaacc ccgacgactg
     7561 ggaactcatc accgcagggc aacgcgtcca aatcattaaa aaagactccg aaaaaggcgg
     7621 cgtgctccag tttggtacgg agattgtcgc ccacgccgac ggctcactcg ccgcattgct
     7681 gggcgcgtcg ccgggcgcat cgaccgctgt gccgctgatg atccggctga tgcaccaatg
     7741 cttccccgag cgcgccccgt cttgggaagg tcgtctgaaa gagctggtac cgggttacgg
     7801 catcaagttg aacgaaaacc ctgaaagggc ggatgaaatt atcgcctata ccgcgaaagt
     7861 gttggatatt taagccgaat ctttcaagaa aagccgtacc cccatatggt gttgatgccg
     7921 tccctgccgg ttatgccctt catccggcag ggcaggtttt ggtacacggg gcaggtaatc
     7981 gttttttccc ttttgtgaat aatggaacag tcaaaaaaca gcgttttcat ttctgaaaac
     8041 gctgtttttt gttgcctgat taacgtgggg aacagtcttt gaagacgatt tggttgtcag
     8101 gtgcggtaat cataatgggc tgtttgcggt aggatttacc atccatcacg ccggtaccca
     8161 aaacataacc gccttctttg ccgtagaatg tttccacatt gtcggatttg tccaaattga
     8221 caggcatttg cacgcgtttg ccgttgatga cggcggaagc gtatgtggtc aggccctgtt
     8281 tgttgaagcc gtaggttact ttgacttttt taccttgctg gcagacgtag ctgacggttt
     8341 ttttggcaac ggtggggttg ttcgtgccgg cagcagcagc catactgctg agcgcgattg
     8401 cggaagacag gattgcggtg gtcagaagtt tcatttttat tcctttggat ggatgtggag
     8461 tttgcccgtt tcgagctgtt tgacattcta atgtcggcgt atcctgaaat aaacaggaaa
     8521 ttggataaag tttgtaaaaa ggcgggttcg ggcggttcgg cggtttgagg caggggcggc
     8581 aggcgggata cggcggcctg tccggtgttt cagacggcat tgtttgcgtt tttcggaaat
     8641 gaaaaacctt cgggcatccg gaggttgcgg tttaactttg tttttttctt tcggacttgg
     8701 tctgtttgga caattcggca tacaggtcgg gatggtgttt tttcaacagg gcggcgattt
     8761 ccatatcttt tttgttgact ttcgccaaat cgatgcattc gatatggacg aggcggagca
     8821 gttcgcgtac gggtttgggc attttgcggc cggtttcgta gcgcgagcct ccagactggg
     8881 taacgccgac cttgccccag aagtctatct ggttcagccc ggtcttttta cggatggctt
     8941 taacatcttc tatattttca aatattttca taaaacttcc tttaagtttg tctggttgcg
     9001 gggcaaattc cgatttgctt tgttttcgta atgtgccggg tcaagcctgt ttttatatta
     9061 tatatcatat ctgccgtttc tttcagacgg cattgtttgt ttttatgggg cggatacgtc
     9121 cggtttcagg gtttgccgga ggcggggctg acggtgagga tttcgtagcc ggtttcggtc
     9181 accaagactt cgtgttccca ttgggcggag agggagcggt ctttggtaac caccgtccag
     9241 ccgtcgttga ggatacgcag gtggcgtttg ccttggttga tcatcggttc gacggtaaaa
     9301 atcatacccg gttttagaac ggggccctgt cctttttttc cgtagtgcaa cacttgcggg
     9361 gcttcgtgga aaccgcgccc gatgccgtgt ccgcagaatt cctgtacgac ggaatagccg
     9421 gcgttttcgg caacctgttg gcaggcgtaa cctacgtcgc ccagtgtcgc gccgggtttg
     9481 acggcttcta tgcccgccat catggaggcg tgggttacgt cgatcaggcg ttgggcgatg
     9541 ggggagactt tgccgacggt aaacatacgg ctggagtcgc cgtggaagcc gtcttttttg
     9601 atggtgaggt cgatgttgat aatgtcgcct tctttgagcg gcttgtcgtc gggaatgccg
     9661 tggcagatga cgtggttgac ggaggtgcag caggatttgg ggtagggcgg gttgccgtag
     9721 tgcagggggg cgggatagcc gccttggacg ttgacgtggt agtcgtaaac gagtttgtcg
     9781 atttcgtcgg tggttacgcc gggtttgacg aattgtccga tgtagtcgag ggcttcggcg
     9841 acgagtttgc ccagctcgcg cattttttcg atttcttcgg gggttttgat gatgatgccg
     9901 ttcataatgc ttccttggat tgcgggtgtg cctgccgtgc cgggtcagaa gaggttgtcg
     9961 agaatttctt tatggggttt gggcggcgtg tttttcgggg tgtccggttt ggtatggttt
    10021 tctttgggcg tttctttttc tttgggtgtt tctttgggct gcgcctgttt ttcgggtgtt
    10081 tcttttcggg gttcgggacg ggggacgttt gtgggcagta cgggggtttc tttgagtgtg
    10141 ttttcagctt gtgttccggc gtcgtccggc tgcctgtcgg tttgagctgt gtcggcaggt
    10201 tgcggtttga cccggttttt cttgggtgcg gcaggggacg gcagtccgct gccgctttcg
    10261 tctgtgccgt ccggctgtgc gggttcggat gaggcggcgt ggtggtgttc gggttgggcg
    10321 gcatcttgtc cgactacgcc gtttggcagc cagaattcgg tttcgcgggg gctgtcggtg
    10381 tgttgcggtt cggtttggag ggttttgccg ccgttcgggt tgaatgtgct gacgatgcct
    10441 attgccaatg cgccgatggc ggtgatacag atgagcaata cgacacgtat caggagtttg
    10501 ggggtcagcc tgaagggttt gttcggtttt tttgccattt tgattgtgct tttggggtgt
    10561 cgggcaatgc cgtctgaagg cggttcagac ggcattgccg agtcagcgtt ggacgtagtt
    10621 ttggtagagg gtgatgactt tttgtacgcc gacggtggtg ctgacttttt gggtaatctg
    10681 cgcctgttct tcgggggtga ggatgcccat aacgtaggtt acgttgccgt aggtaacgat
    10741 tttgacgcgc gcctgtgtgg cggggctgat gcccaacagc gtggcgcgga ctttggatgt
    10801 gttccaagtg tcgccggcga tgtcgccggc agtgcgcggc agggaggcga cggtaatgta
    10861 gttgtatacg ccttcggcgg cctgttcgga acgtgcaatc tgaccgacga actgtttctc
    10921 gccttcggtg gcgacttgtc cgagcagcag caggtggcgg ttgtagccga caacggagat
    10981 ttggggcgtg tagcctttgg tttggttgtt ttggcgcaga taggagcggg cggtggtttc
    11041 gatacgcaac gccataacgt tgtcgtcggt ttgcgcgccg gtggttcggc ggtcgacggc
    11101 ggatttcgcg ccgacggcgg cgcttccgat tactgcgctg acgcagccgc taagggcaag
    11161 gctgaaaatg gcggcaatca gggtgcggac ggtgtgcggt ttgggtttca tcgggtgctt
    11221 cctttcttgg gcgtttcaga cggcattgct ttgcgccatg ccgtctgaaa agggttacat
    11281 tccttccagc agtacggagt cgatacagtc gcacatggcg tgtatcagca ggatgtggtt
    11341 ttcttgaata cgggcggtgc gcggatgggg aacgttgagc aaaacgtcgg tgtctttgag
    11401 tatggcggcg attttgccgc cgtcgcggcc ggtcaaggcg atgacgtgca tatcgcgttc
    11461 gtgtgcggct ttgacggctt cgatgacgtt ggcggaattg ccggaggtgg aaatgccgac
    11521 caatacatcg cctgcacgtc cgagcgcgcg cacctgtttg ctgaatacgt ggtcgaaacc
    11581 gtagtcgttg ccgatggctg tcagcgcgga agtgtctgtt gtcagcgcga cagcggcgag
    11641 ttccatgcgt tctttttcaa aacggccggt catttcggcg gcgaagtgtt gcgcgtcggc
    11701 agccgaaccg ccgttgccgc aggccaggat tttgccgtca ttcatcaggc attgcagcat
    11761 cagctcggca gcctgtacgg tcggctcgac caatactttt ccggcttcct gcttggcacg
    11821 gatgctttcg gcaaaatggg cggcaacgcg ttcttgtaat gtcgtcatat cgtcaacctg
    11881 taatattctg tatccactcg ggcgggcggc tgccttcgat gagtaccgcg tcgaggcggc
    11941 acggtacgtt tgtcaacctg ttctgttgca gataatactc tacacttcgt tgcagtttca
    12001 ataatttgga tggggaaatg ctgtatgcgg caccgccgaa ttgccgattt ttgcggtatt
    12061 ttacttcaac aaacagaatc atgccgccgt ttttgacaat caggtcgatt tcgccgtagg
    12121 cgcagtgcca gttgcgggca agcagtgtgc agccttggga ttggaggaag gcaagcgcgg
    12181 catcttcccc tgcctcgccc tgtttgtggt ttaggcgcat aatcggttta acctttataa
    12241 tgtttgtttt tcagacggca ttcttatgtt tcagaaacat ttgcagaaag cctccgacag
    12301 cgtcgtcgga gggacattat acgtggttgc cacgcccatc ggcaatttgg cggacattac
    12361 cctgcgcgct ttggcggtat tgcaaaaggc ggacatcatc tgtgccgaag atacgcgcgt
    12421 taccgcgcag cttttgagcg cgtacggcat tcagggccaa ctcgtcagcg tgcgcgaaca
    12481 caacgaacgg cagatggcgg acaagattgt cggctatctt tcagacggca tggttgtggc
    12541 acaggtttcc gatgcgggta cgccggccgt gtgcgacccg ggcgcgaaac tcgcccgccg
    12601 cgtgcgtgag gtcgggttta aagttgtccc tgttgtcggc gcaagcgcgg tgatggcggc
    12661 tttgagtgtg gctggtgtgg cgggatccga tttttatttc aacggttttg taccgccgaa
    12721 atcgggcgaa cgtaggaaat tgtttgccaa atgggtgcgg gcggcatttc ctatcgtcat
    12781 gtttgaaacg ccacaccgaa tcggggcaac gcttgccgat atggtggaat tgttccccga
    12841 acgccgatta atgctggcgc gcgaaattac gaaaacgttt gaaacgttct taagcggcac
    12901 ggttggggaa attcagacgg tattgtctgc cgacggcaac caatcgcgcg gcgagatggt
    12961 gttggtgctt tatccggcgc aggatgaaaa acacgaaggc ttgtccgagt ccgcgcaaaa
    13021 catcatgaaa atcctcacag ccgagctgcc gaccaaacag gcggcggagc ttgccgccaa
    13081 aatcacgggc gagggaaaaa aagctttgta cgatctggca ctgtcttgga aaaacaaatg
    13141 atgccggacg gaccggcacc atagtgcgaa cggaatcaac ggacacgccc cgtcatgcgg
    13201 tacagcacgt ctttgccttg aacacggtgg acgacgacca tggcgacgtg tccgacgacg
    13261 gcggcaaaca gcagccagcc caaattgccg tggaacgtgt tgcccaagtt tgccatccac
    13321 tcgatttttt ccggcgaacc ctgcatcact tcaacgccga acactttcaa cgggccgcgg
    13381 ccgctgccgt attggcggat catgccgata acgggaacgg caagcatgag cagatacaga
    13441 atgccgtgtc ctgccgccgc agccttgctg tcgctttgcg gacgcttggc gcggttggca
    13501 accgcccaca cgatgcgcaa tgtaatcacc gtcagcgtaa ggaaaccgaa agatttgtgc
    13561 aggccgaaca ggctgcccac ccattccgcc tcttcgtata gtggattaac aaaaaccagt
    13621 acggcgttgc ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct
    13681 gatttttgtt aatccactat aaatcgtcca caggacaatg gtggtcggaa tgccgatgaa
    13741 accggcaacg gtcagccagt gcagcgtacg tgtcaacggg tcgtaaacgg caggttggtt
    13801 tgtgttcata aaaaacttag gggctgtcct agataactag ggaaattcaa attaagttag
    13861 aattatccct atgagaaaaa gtcgtctaag ccggtataaa caaaataaac tcattgaact
    13921 gtttgtcgca ggtgtaactg caagaacggc agcagagtta gtaggcgtta ataaaaatac
    13981 cgcagcctat tattttcatc gtttacgatt acttatttat caaaacagtc cgcatttgga
    14041 aatgtttgac ggc
//
LOCUS       DAWHXS010000055        13498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWHXS010000055 DAWHXS010000000
VERSION     DAWHXS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.055"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(266..817)
                     /locus_tag="WIE22_001242"
     CDS             complement(266..817)
                     /locus_tag="WIE22_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ4127324.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQLWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSELACKTVETEARLEELY
                     NRKKALIDEMVREADKKELPKRL"
     gene            complement(831..2477)
                     /gene="pgi"
                     /locus_tag="WIE22_001243"
     CDS             complement(831..2477)
                     /gene="pgi"
                     /locus_tag="WIE22_001243"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ4127325.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            complement(2598..3446)
                     /gene="hexR"
                     /locus_tag="WIE22_001244"
     CDS             complement(2598..3446)
                     /gene="hexR"
                     /locus_tag="WIE22_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ4127326.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(3496..4482)
                     /locus_tag="WIE22_001245"
     CDS             complement(3496..4482)
                     /locus_tag="WIE22_001245"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ4127327.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKASPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            complement(4463..5158)
                     /gene="pgl"
                     /locus_tag="WIE22_001246"
     CDS             complement(4463..5158)
                     /gene="pgl"
                     /locus_tag="WIE22_001246"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ4127328.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            complement(5438..6883)
                     /gene="zwf"
                     /locus_tag="WIE22_001247"
     CDS             complement(5438..6883)
                     /gene="zwf"
                     /locus_tag="WIE22_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ4127329.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            7399..9234
                     /gene="edd"
                     /locus_tag="WIE22_001248"
     CDS             7399..9234
                     /gene="edd"
                     /locus_tag="WIE22_001248"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ4127330.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            9415..10053
                     /locus_tag="WIE22_001249"
     CDS             9415..10053
                     /locus_tag="WIE22_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ4127331.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            10068..10286
                     /locus_tag="WIE22_001250"
     CDS             10068..10286
                     /locus_tag="WIE22_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127332.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(10310..11255)
                     /locus_tag="WIE22_001251"
                     /pseudo
     CDS             complement(10310..11255)
                     /locus_tag="WIE22_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            11418..12458
                     /gene="mutY"
                     /locus_tag="WIE22_001252"
     CDS             11418..12458
                     /gene="mutY"
                     /locus_tag="WIE22_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ4127333.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            12875..13435
                     /locus_tag="WIE22_001253"
     CDS             12875..13435
                     /locus_tag="WIE22_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ4127334.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         3129 a         3680 c         3419 g         3270 t
ORIGIN      
        1 caacgctgta tcggtttaaa tttaattcac tataaaaacg acaaaaacgc aaaagccgcc
       61 gacattcccg catccaagtt tcagtcaatc agataacctt ggatttcttt ggttttcgca
      121 ttgatttctc tggtacggca gtcagattgt gccacgccgt attcgtcgcc gtcggcgcat
      181 ttggcattca aaccgttttg tcagttgcgg tactcgggca tgacggtttc ggcaatatcg
      241 gcgggcagtg cctgaaccgc gctgttcaga gccgctttgg cagttctttc ttgtccgctt
      301 ccctgaccat ttcgtcgata agggcttttt tacggttgta cagctcttcc aagcgtgctt
      361 cggtttccac cgttttgcat gccagttcgc tgacttttat gccgtttggc gtttcacctt
      421 cggctttcgc attgttggca cagattttgt ccataccgct tttccatgtt ttctgtgagg
      481 cttgcagctt attctgtacg gtttgaggca atcctttcca gagctgctcg aactcgctgc
      541 gcgacagctt gtagcgttct tcccattcgg atacgcggtc gacttcctgc tcccgaccca
      601 atgcctcctg cgccgccgct tcttctgccg cttcaagttc ttcgttcctt tgtttcgctt
      661 tgtccaacgg ctctttaatc agtgccatag ctgcggaaca tatatgttta aatttatgca
      721 aaccatcata tcgggattgc acacgctctg caagtttacc gacggttttc ctgttcgata
      781 aaaatgccgt ttgaaacggt cggcgttcag acggcatttt tccgcaggtt ttatttgcgg
      841 ttggtctgca ggtagaggtt gatcaggcgt tcggtcgagc tgtcgtgctt ttgcgacccg
      901 gtttcgccgg tcagttcgcc caaaatggtt ttagccagtt gtttgccgag ttccacgccc
      961 cactggtcga agctgttgat gccccaaatg atgccttgta cgaaggtttt gtgttcgtac
     1021 atggcaatca ggctgcccat attacgcggg ttgaccttgt ccatcaggat gaggttggtc
     1081 gggcggttac cggagaaggt tttgtgcggg accagctctt cgatgcgcgc ctcatccata
     1141 ccctgcgctt tgagttcggc gcggacttcg tcgggggttt tgccgcgcat aaaggcttct
     1201 gcttgggcga agacgttggc aagcaggatt tcgtggtgtc cgggcaggtt gctgcgtttt
     1261 tcaagcgagg caatcaggtc gatgggggta atgtgcgtgc cttggtgcag cagttggaag
     1321 aaggcgtgct ggccgttaat gcccgtttcg ccccagataa tcggcgaggt ttcgtgtccg
     1381 actgctttgc cgtccaacgt aacctgtttg ccgttacttt ccatatcgag ctgctggatg
     1441 aatttgggca ggcggtgcaa atgttggtcg taaggcgcga tgacgtggct gccgccgccg
     1501 tagtagttga tataccagat gccgatgagg gcgagaatga cgggcaggtt gcgctcgagc
     1561 ggtgtgttga tgaagtgttg gtccatcagg tgcgcgccgt tgagcatttc aatgaagttt
     1621 tcttcgccga gatacagcat aatcggcaat ccgatggcgg accacaggct gtaccgaccg
     1681 ccgacccaat cccaaaattc aaacatattg gcggtgtcga tgccgaattc ggcgacggct
     1741 ttttgattgg tggaaacggc ggcgaagtgt ttggcaacgg cttcttcgtc gcccgcatga
     1801 ttcaaaaacc attcgcgcgc ggtcagcgcg ttggtcagcg tttcctgcgt ggtaaatgtt
     1861 ttggaggcga tgatgaacag cgtggtttcg gggtggactt tggacaatac gtcgcgcagt
     1921 tgggagccgt ccacgttgga gacgaagtgc atattgaggc gcggatgacc gaaaggtttg
     1981 agcgcggtac acatcatcag cgggcccaaa tccgatccgc cgatgccgat gttgacaacg
     2041 tcggtaatga cttggttggt atagcccagc cagcttccgc tgcggacttc gtgtgcaaat
     2101 tcgcccatac gttgcaaaac gcggttgact ttgggcatca catcttcacc gtcaaccaca
     2161 atcggcgaat tggtgcggtt gcgaagggcg acatgcagga cggcgcggtt ttcggtggta
     2221 ttgatttttt cgccgtggaa catctgccgc atccgctccg gcacgcctgc ttctcgggca
     2281 agctcgaaca aaagcgacat ggtttcgtcg ttgatgcggt ttttggagta gtccagcgtc
     2341 agtccgccga cttgcagcca gtagcgttcc gcacgctgcg ggtcttgctc gaacatttcg
     2401 cgcatatgca atgttttgct gtcgtcaaag tggttccaca atttcgacca tgcgggtaag
     2461 tcgtgaaagt gtttcatcta tatgctcctg aatgaggttt tttgttgtgg gatgaaaagg
     2521 ctgctggaaa ctgccgcaag ccgccgacgg ccgttgttcg gcatttcaga cggcatttgt
     2581 gggatgccgt ctgaaggtca atctttgtcg taatcgatgt gcttgttgtg tatgcttttt
     2641 ttgcttttct gcaattgcag gctggcagca tcgcccaagc gcagggcaag tccgatggcg
     2701 agaatgtcga tgacggcaag ctgcaagagg cgggaaacca tgggcgtgta gagttcggca
     2761 ttttcctgtg tggcaacgct caacacgcag tcggcaagtt gcgccagagg cgaatcgttg
     2821 cgggtcagtg cgatgacgga cgcgccgttt tctttggcga tgctgaccgc atccaaaagt
     2881 tcgatagacg aacccgtgtt ggaaatggca accaaaacat cctgatcgct caaaacggat
     2941 gctgccatca gctgcgtgtg cgtatcgaca taggcgaccg tggacattcc gaaacggaaa
     3001 aacttgtgtt gcgcgtcctg cgcaacgata ccggaattgc cgacaccgta aaactcgacg
     3061 cggcgggcgt gcatcagcgt ggcaatggcg ttttccagct ccgactcttt caggaagcgg
     3121 cgttcgccca acagcgaggc ggcggcattg cccaacactt tctcgaccac gcttgccata
     3181 tcgtcgtcgg cgttgagttc ttcgtggaca tagggcatac cctcatgacc gatgctggcg
     3241 gacaaggcga gcttgaactc gggcagccct ttataaccca agctgcggca gaatcggatg
     3301 acggtcggct ggctgacgga cgcacgttcg gcaatttcgg caacggcggc atggacgaac
     3361 catttgggtt ccgccaatgc acattcggcg actttgcgtt ccgcaccgga aaggtttgcc
     3421 agtgattcgc tgattttgct taacataatg atatgccctt cgataatgca gccccgctgc
     3481 aaggagccgc tgtggttaaa cgtttctcaa atggttgtcg agagccgcag ccgcaccgga
     3541 aattccggga aactcgctca agacgacata cacgggaatc gcggcaagat atgcttcaaa
     3601 cctgcccttg ttctcgaaac ggctgcggaa cggggaagct ttgaaatatt ccaacacgcg
     3661 gggaataatg ccgccacaca ggtacacgcc gccgcgcgca cccagcgtca gggcgaggtt
     3721 ggaagcaacc gtgccgagca tggcgcagaa gatgtccaaa gtctgacggc acaaaggcga
     3781 cgcgccgctc aaagcctttt ccgtgatttc agacggcatc agtttggcgg gtttggcttt
     3841 ctgttttgca gccaaagcct cgtaaaccaa gctcaagccc gcgccgctca aaaagcgttc
     3901 ggcggaaaca tggccgtatt tgtttttggc gtactgccaa atcagcactt ccatatcgtc
     3961 aaacggcggg aaactggtat gcccgccctc gcccgccaaa gccacccagc ctgcgtggct
     4021 gtgcaccaat ccgctcacgc ccaggccggt accggggccg ataacggctt tgggggcaaa
     4081 ttcgacaggc ttttgcccgc ctacctgcat caggtctttg cttgaagtct gcgttaccgc
     4141 caatgcctgc gcggtaaagt cgttcaaaag gatgagggtg tccagcccca aagtctgacg
     4201 ggtggtttcg atggaaaacg cccaatggtg gttggtcatc tgcacccagt cgcccaaaat
     4261 cgggttggcg atggcaaatg ccgcgtgccg tacggctgtt gcaccgcttt gattcagata
     4321 ggcacgcacc gcatcggtaa ccgtatcgta gtctttacac ggaagcacgg cggctttttc
     4381 aatgacgcgc ggcgcggttt ccagcgcaaa gcgtgcattc gtcccgccga tatcggcgac
     4441 cagtcgggga tatccggctt gtttattcgg cgtagaagac atggcagttc actccttgat
     4501 ggttcaaaac gaggttgatc ggatattcgc ggttttcgcc ttgtgcggct tggtcgaaca
     4561 cggctttttt ctcttcgccc cgtatcgcca aaaacacatg ccccgtatgg gcaatcgcat
     4621 ccaaggtcat actgacgcgc tcgtgcggcg cggtaacggg cgtggtatgc accagcgcga
     4681 cacctgccga accgtcgatt gccgtctgaa actgcggagc tttcgggaaa atcgaagccg
     4741 tatgcccgtc gtttcccata cccaaaacca aaacatcggg ctgtttgtaa tgtttcagtg
     4801 cataatcgac aacagcatcg ggatgtaatt cggtttcagt ttttccgtct tccaccatag
     4861 gaatccacat tgccgcttcc gctttgttct tcaacaggta ttcgcgcacc aaaccggtat
     4921 tgctgtcggc gtggacggtc ggcacgatgc gttcatctgc caaggtgatg ccgacgtttt
     4981 tccaatccaa atctttttgc gacagggcgt tgaaaaatgc aatcggcgaa cgtccgccgg
     5041 aaactgccaa caccgcaccg cccttctcgt ccagtgcgcc ctgcaaagca tccgccactg
     5101 cgtcagccaa agactgcgcc gcttctgccg cattttcgta ttcgtgccaa acaaacatat
     5161 ttgtgtcctt tttttatttc agacggcata ttccgttatg gaaacgggtt gagcaatatg
     5221 ccggtcgaac agttgtttat gcttttgata ccaaatatcg ggactgcttt ttatagtgga
     5281 ttaaatttaa accagtacag cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc
     5341 ttcgtcgcct tgtcctgatt tttgttaatc cactatactg ctttacttgc gttcagacgg
     5401 catttcaaac cccatgccgt ctgaacgcat tattgtatta ctgctcttcg tgccacttgt
     5461 gtccgtcgcg cgccaatagt tcgcgcgcgg cttcaggccc ccacgagtgt gcgccgtagc
     5521 cgtgcggcgg cgtggtgtta tttgcccagt tttccaaaat cggcatcaca tattcccacg
     5581 cggcttcgag ttcgtcgcgg cggttgaaca aagcgagttt gccgttaatc acatccagca
     5641 gcaggcgctc gtaagcttcc gcgcggcggc cttccaatgc tttgcccaaa tcggttgcca
     5701 gcggcacggt ttcgacctta tttcctgccc ccggggtttt catctgcgta tagaggcgca
     5761 cggattcata tggttgcaac tcgataacga gccggttggg cgcggtgcga ctgccttcaa
     5821 aaatatggct gttcaaatct ttgaagttca aaacgatttc cgccactttg cccgccatgc
     5881 gcttgccggt acgcaggtag aagggaacgc ccttccagcg ttcgttttcg atttcggctt
     5941 taatggcgac gtaggtttcg gtaaagctgt cttgcggaac gttgatttct tcaagatagc
     6001 cgttcatgcc tttggcggcg gtatattgtc cgcgcacgac attttcattg acagattcga
     6061 cggtcagcgg cttcaatgac ttgatgactt tgactttttc atcgcgcacc gcgtcggcat
     6121 ccaagctggc gggggcttcc atcgcagtca tgcacagcat ctgcatcaaa tggttttgca
     6181 ccatatcgcg caacgcgccg gtaatgtcgt aaaactcgcc gcgctcttcc acaccgagct
     6241 gttcggcgat ggtcaactgc acgctttcga tgtatttatt gttccacagc ggctcgaaca
     6301 ttacattggc aaaacgcagc gcaagcaggt tttgcaggct ttctttgcca aggtagtggt
     6361 cgatgcggta aatttgccct tctttgaaat aacgcgcaac atcggtattg atttgctggg
     6421 aagaagccaa atccgtaccc aacggttttt ccaaaaccac acgcacgttg tcggcgttca
     6481 aaccgatttt ggcaaggttt tcacatgttt gcgcgaagaa tttcggcgcg gtggaaagat
     6541 agatgacgac attgtcggtt tctttgcgcg ctttgaccaa atcgcccaaa gcggcaaaat
     6601 cgtccggctg cgtaacatcg actttgagat atgcgagacg ttcgacaaac gatgcccaag
     6661 cctcatcgga aaaattttct ttcacatgga ttttggaact ggtttccacc ttcgccagaa
     6721 aaccttcggt atccaactcg ctgcggctga cccctaaaat acgcccttcg ggatgaagca
     6781 gaccggcaac atgcgcctgg tacagacagg gcaacagctt gcgcatcgcc aaatcgccgg
     6841 tcgcaccgaa caacaccaaa tcaaaatttg tttgtgtact catcgtatta tctcgtcagg
     6901 aaagaatttt tcgatgccgt ctgaaacctg tttcccccat cacgctgcat cgcaatatcg
     6961 gaaacaaagg caggcggcat aatgagtagt aatactacac accgctacac tttttgtcta
     7021 ttcccatttt tacaatttat ttgacctagt ccaaaaatcg ggcaggtttc ccctattccg
     7081 ttacaacaat cggaagattc tgcgatttaa atcaaatttc ttttcgatgt ctgatttttt
     7141 tgtaacaaaa ttacaaattt tgtactataa tagcacccgc ttcccacttt cagacggcat
     7201 accttttaaa atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
     7261 acagataata cggcaaggcg aggcaacgcc gtactggttt ttgttaatcc actatactca
     7321 ccgtctgaat acccgataca aaaatcagaa acgcacaaac aaatccccaa taccccccgt
     7381 tccgacagga gaccgaccgt gaacactact cctatccact ccaaactcgc cgaaatcacc
     7441 gggcgcatta ttgaacgcag ccgtccgacg cgtgaaaaat atctggcgaa gatccgcagt
     7501 gccaaacaaa tgggacgctt agagcgcaac cagctcggtt gctccaactt ggcgcacggc
     7561 tatgctgcca tgcctaaaag tatcaaaatc gaaatgcttc aggaaaccgt ccccaactta
     7621 ggcatcatca ccgcctacaa cgacatggtt tccgcacacc agccgtttaa agacttccct
     7681 gaccaaatca aagacgaagc gcagaaaaac ggcgcgaccg cccaagtcgc cggcggcaca
     7741 cccgccatgt gcgacggcat cacgcaaggc tacgccggta tggaactgtc gctgttctcc
     7801 cgcgacgtga ttgccatgag taccgccgtc gggctgtcgc atcaaatgtt tgacggcagc
     7861 ctgtttatgg gcgtatgcga caaaatcgtt ccgggtttga tgataggcgc gctttcgttc
     7921 ggtcatattc cgggtatctt cgtccccgca ggcccgatgt ccagcggtat cggcaacaaa
     7981 gaaaaagccc gcacccgcca gcttttcgcc gaaggcaagg tcggacgcaa cgaacttttg
     8041 aaaagcgaaa tgggttctta ccacagcccg ggcacctgca ctttctacgg cacggcaaac
     8101 tccaaccaaa tgatgatgga aatgatgggc gtgcacctgc ctgccgccgc cttcgtccac
     8161 ccttacaccg acctgcgcga agccctaacc cgctacgccg ccggacacct cgcgcgcggc
     8221 atcaaaaacg gcacgattaa acctttgggc gaaatgttga ccgaaaaatc ctttatcaac
     8281 gccttgattg gcctgatggc aaccggcggt tcgaccaacc acaccatgca cctcgttgcc
     8341 atggcacgcg ccgccggcgt gattttgaac tgggacgact tcgacgaaat ttcctccatc
     8401 atcccgctgc tcattcgcgt ttatccgaac ggcaaggccg acgtgaacca ctttaccgca
     8461 gcgggcggac tgcctttcgt tatccgcgaa ttgctgaatg caggcctgtt gcacgacgat
     8521 gtcgataccg tcgtcggaca cggtatgcgc cactacacca aagagccttt cctcatcgac
     8581 ggcaaactcg aatggcgcga agcccccgaa accagcggca acgacgacat cctgcgtaaa
     8641 gctgacaacc cgttctcccc cgacggcggt ctgcgcctga tgaaaggcaa catcggacgt
     8701 ggcgtgatta aagtgtccgc cgtgcgcgaa ggctgccgca ttattgaagc gcctgccatc
     8761 gtgttcaacg accaacgcga agtgttggct gcgtttgaac gcggcgagtt ggaacgcgat
     8821 tttgtgtgcg tcgtccgcta ccaaggcccg cgtgccaacg gtatgcccga attgcacaaa
     8881 ctgaccccgc ctttaggcat cctgcaagac cgcggcttca aagtggcgct gctgaccgac
     8941 ggccgtatgt ccggcgcgtc cggcaaagtt ccagcctcca tccacatgac acccgaagcc
     9001 ctgatgggcg gcaacatcgc caaaatccgt accggcgacc tgatccgctt cgactccgtt
     9061 accggcgaac tcaacgtcct gattaacgaa gccgaatgga acgcccgaga agtcgaaagc
     9121 atcgacttgg gcgcgaacca acaaggctgc ggccgcgaac ttttcgccaa cttccgcagc
     9181 atgaccagca gcgccgaaac cggcgcgatg agcttcggcg gcgaatttgc ctgatgcgcg
     9241 tttcagacgg ccttttcaga ccgaaggccg tctgaaaaat tattcaagtg ttttaagata
     9301 gacgtaggtc ggattctcga atccgacaca gccgtccaag atgtcggttt cttgaatccg
     9361 acctacaacc tgtcccatct taataaaata ccccatccca cccggagaac cgaaatgtcc
     9421 aaactgaccc cccgcgaaat cctgaccgcc ggcgcagttg taccggtaat ggcgattgac
     9481 gacttgagta ctgccgtcga tttgtcccac gcccttgtcg aaggcggcat ccccaccctc
     9541 gaaatcaccc tgcgcacccc tgtcggcctc gatgccatcc gcctgattgc caaagaagtg
     9601 cccaacgcca tcgtcggcgc gggtacggtt accaaccctg aacagctcaa agccgtcgaa
     9661 gacgcaggcg cggttttcgc catcagcccg ggtttgcacg aatccctcgc caaagccggc
     9721 cacaacagcg gcatccccct gattcccggt gttgccaccc cgggcgaaat ccaactggct
     9781 ttggaacacg gcatcgacac cctcaaactc ttccctgccg aagtcgttgg cggcaaagcc
     9841 atgctcaaag ccctgtacgg cccttacgcc gatgttcgct tctgcccgac aggcggcatc
     9901 agcctcgcca ccgcgcccga ttacttggca ctgcccaacg tcctgtgcgt cggcggctca
     9961 tggctgacgc cgaaagaagc cgtgaaaaac aaagactggg acaccatcac ccgtctcgcc
    10021 aaagaagcgg cggcattgaa acccaaagcc tgaaccgcat cgtaaaaatg ccgtctgaag
    10081 ccctttccag tctcagacgg cattttgccg cttggcggcg cagtcggcat acacggcagc
    10141 accactccga catactgccc ctaaaatgcc catccgcctt ccgcataata aaaataacgt
    10201 tcagttcatt cgacagcagc cggacagccc atactacgcg gctgaaaaaa tgccgtctga
    10261 aacgcattca gacggcatcc gcttaaaaaa acaactgatt caaggccgat taatccgctt
    10321 ccaaaaccac tttcatcgct tgattttcgg cggcgtgttt gaacacgtcg taggcttttt
    10381 ccaattcgct gaatttgaaa cggtgggtca acattttggt gtaatcgacg gagctgctgg
    10441 aaattgcctt catcagcatt tcggtggtat tggcgtttac caggccggta gtgatggcaa
    10501 gctttttaat ccacagtttt tccagtttga aatcaacgga ttgaccgtgt acgccgacaa
    10561 cggcgatatg gccgccgggt ttcacaatgt cttggcacat attccaagtt gcagggatac
    10621 cgacggcttc gatcgcgcag tccacgccgt cttcgccgac gatagcgaca acttgcttgc
    10681 ttacatcgcc ggaagcaggg ttaatggtat gggttgcgcc caactctttc gccagtttca
    10741 aacggttttc gtccatatcg caaacaatga tggcggcggg gctgtacagt tgggcggtcg
    10801 gcaaggcgtc gctcaacaac agggcgactt cttcgttgac gttgtcgggc agcggcacga
    10861 ggctgttgtc ggcataaggc gtgcggacgt attctgcctg cgtgccgtca atcatgtagc
    10921 ccaaaatcca gccgccgttg cggcaatgcg aataaagttg gattttgcag ttgtcgcaag
    10981 tacagcattt gctgacgcat gaaataatga ctttatcgcc gactttgatg ttttttacag
    11041 cctcgccgac ttcttctaca ataccgatgc cttcatgacc gagaatacgg ccgtcggcga
    11101 cttcgggttt ttttgccttt ccaaataccc aaatcggtac cgcaaatcgt ggttttgacg
    11161 attttcacca ccgcatcggt cggatcgata atctgcggac ggggtttttc ttcaaaacgg
    11221 atgtcgtttg cgccgtgata aaccattgct ttcatgctga tactccttgc ttgttgataa
    11281 ataatttcaa taccgcaata aagtttcctt atatgagtta tatgccccta caaaaaataa
    11341 gtcaataaga attattttca caatgttata caataacata ctattttaaa tataaataaa
    11401 accaccgatt gatattaatg aacacaccca tccccttctc cgaacggctc atccgctggc
    11461 aaaaacaaca cggtcgccac cacctccctt ggcaggtcaa aaacccttat tgcgtctggc
    11521 tttccgaaat catgctccag caaacgcaag tcgccaccgt gttggactac tatccgcgct
    11581 tcttagaaaa attcccgacc gttcagacgc ttgccgccgc gccgcaagac gaagtgttgt
    11641 cgttgtgggc gggcttgggc tattacagcc gcgcgcgcaa cctgcacaaa gccgcgcaac
    11701 aagtcgtcag gcaattcggc ggcacgtttc cgtcggagcg caaagacttg gaaaccctct
    11761 gcggcgtagg cagaagcacc gccgccgcca tttctgcctt ctccttcaac cgccgcgaaa
    11821 ccattttgga cggcaacgtc aaacgcgtac tctgccgcgt gttcgcccgc gacggcaatc
    11881 cgcaggacaa aaaatttgaa aactcgctct ggacacttgc cgaaagcctg ctgccgtctg
    11941 aaaacgccga tatgcctgcc tatacacaag gtttgatgga tttgggcgcg accgtgtgca
    12001 aacggacgaa acccttgtgc caccaatgcc cgatggcgga catctgcgaa gcaaaaaagc
    12061 aaaaccgcac cgccgagctg ccgcgcaaaa aaaccgccgc cgaagtaccg accctgccgc
    12121 tttactggct gattgtccgc aaccgggacg gcgcgatttt gctggaaaaa cgccccgcca
    12181 aaggcatttg gggcgggctg tattgcgtgc cgtgttttga aagtttgaac gggctttccg
    12241 actttgccgc caaattctcc ctgaccatgg cagatatgga cgaacaaacc gccctgaccc
    12301 accgcctgac gcaccggctg ctattgatta cgccctttga agcacaaatg ccgtctgaaa
    12361 gcccttcaga cggcatttgg ataaagccgg cgcatttgaa agattacggc ctgcccaagc
    12421 ctttggaaat ttatttaaac ggtaataggt tagaataaac aaaataaacc cattgaactg
    12481 ttgtttgcag gtatcgcagc aagaacaacc gatgaatttg ggtcgtattt taggcggcgg
    12541 gataatgttc aaatgggaca tttggaacgg aagaagtcgg caatttaaaa aggatttaaa
    12601 aagcaaagaa ggtcaaaaac atgaacacaa acttaaatga caaagacaaa gccatggata
    12661 ccgcaatcag gtttcagaaa aggatgagga ttccgaaatt tttcttttta attctcggaa
    12721 tcacaatggt tttggcattt atccaagacg tgataacggg ttctaatttt ctgcaaataa
    12781 caattaatgt aaaattttcg taaaaattta tcggctttta aaacaaaatt gactaaaata
    12841 gtcgcgagtt tttactgcaa taaaggagat tgcaatgaat atgaaaacct tattagcact
    12901 agcggttagt gcagtatgtt cagttagtgt tgcgcaagca cacgagcata atacgatacc
    12961 taaaggtgct tctattgaag tgaaagtgca acaacttgat ccagtaaacg gtaacaaaga
    13021 tgtgggtaca gtgactatta ctgaatctaa ctatggtctt gtgtttaccc ctgatttaca
    13081 aggattaagc gaaggcttac atggtttcca catccatgaa aatccaagct gtgagccaaa
    13141 agaaaaagaa ggtaaattga cagctggttt aggcgcaggc ggtcactggg atcctaaagg
    13201 tgcaaaacaa catggttacc catggcaaga tgatgcacac ttaggtgatt tacctgcatt
    13261 aactgtattg catgatggca cagcaacaaa tcctgtttta gcaccacgtc ttaaacattt
    13321 agatgatgtt cgcggtcact ctattatgat ccacacgggt ggtgataatc actccgatca
    13381 tccagctcca cttggcggtg gcggcccacg tatggcatgt ggcgtgatta aataattcga
    13441 ttgttcgaaa cgaaaagtgc ggtgaatttt gaccgcactt ttttgctaga tatttagc
//
LOCUS       DAWHXS010000056        13365 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWHXS010000056 DAWHXS010000000
VERSION     DAWHXS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13365
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.056"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            83..196
                     /locus_tag="WIE22_001254"
                     /pseudo
     CDS             83..196
                     /locus_tag="WIE22_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated molecular chaperone Hsp33"
     gene            complement(282..1736)
                     /gene="mgtE"
                     /locus_tag="WIE22_001255"
     CDS             complement(282..1736)
                     /gene="mgtE"
                     /locus_tag="WIE22_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ4127335.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(1968..6441)
                     /gene="mspA"
                     /locus_tag="WIE22_001256"
                     /gene_synonym="ausI"
                     /pseudo
     CDS             complement(1968..6441)
                     /gene="mspA"
                     /locus_tag="WIE22_001256"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
     gene            complement(6748..7506)
                     /gene="gloB"
                     /locus_tag="WIE22_001257"
     CDS             complement(6748..7506)
                     /gene="gloB"
                     /locus_tag="WIE22_001257"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ4127336.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            complement(7601..11563)
                     /gene="purL"
                     /locus_tag="WIE22_001258"
     CDS             complement(7601..11563)
                     /gene="purL"
                     /locus_tag="WIE22_001258"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ4127337.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGRLNDDEKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPCLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            complement(11720..11932)
                     /locus_tag="WIE22_001259"
     CDS             complement(11720..11932)
                     /locus_tag="WIE22_001259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127338.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRTETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            12027..12365
                     /locus_tag="WIE22_001260"
     CDS             12027..12365
                     /locus_tag="WIE22_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ4127339.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            complement(12424..13182)
                     /locus_tag="WIE22_001261"
     CDS             complement(12424..13182)
                     /locus_tag="WIE22_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4127340.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLACTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
BASE COUNT         2507 a         3218 c         4228 g         3412 t
ORIGIN      
        1 aggattaagg gggtatttgg ggcgaaaaca gctgaaaaca gccgaaaacc tgtgtttggg
       61 tttcggctgt cgtgagggaa aggaattttg caaaggtctc agcaaatatg tgtttgacga
      121 aaccgatgtc aacgcgctgt tcggggagga tgtggtcggc gttgccaaag ggctgccccg
      181 gcataccgtc caataatctt gtgcaacaag gataaatagt caaatgccgt ctgaagcagc
      241 ttccgcttca gacggcattt ttgcgcgggt tcagacggca tttaaagcag gaacagggtg
      301 gcgagaccca agaaaatcag gaagccgccg gagtcggtaa cggcggtaat cagcaccgag
      361 ctgcccagtg cgggatcgcg tccgaacttt tccattacca cgggaattaa tacgccgacg
      421 gttgccgcca gcaggaggtt gagcgtcatc gcggcaatca taaccagccc gatgccgagg
      481 ctgccgtaga gcagccatga aaccgcgccc atgacggttc cccagataat gccgttgact
      541 agggcaacgc cgacttcttt tttcaacagc ctgcctgcct gcatgcctgt cagttgcccc
      601 atcgccatgg cgcggacaat catggtaatc gtctggttgc ccgagttgcc gccgataccg
      661 gcgacgatgg gcatcagcgc ggcgagtgcg acgatttttt cgatgctgcc ttcaaacgcg
      721 ccgatgacgc ggctggcaag gaaggcggtg cagaggttga cggcgagcca catccagcgg
      781 tttttgacgg aatccaagac gggggcgaat aagtcttcct cttcttgcaa acccgccatg
      841 ttcaacatat ctgcttcgga ttcttcgcgg atcacgtcca ccatttcgtc aacggtcacc
      901 ctgccgatga gctttttgtt ttcatcgacg acgggcgcgg taaccaagtc atagcgttca
      961 aacgcctgcg ctgcttcttc cacgtcgtct tcggcgcgga aacgcacgac atctttcgcc
     1021 atcacgtttt ccaccaagtc ttcgggatcg gcgaccaaaa gtttgcggat gggcagcacg
     1081 ccctgcaaca catcgttttc gtcaaccacg aagattttat ccgtgtggtc gggcaggctg
     1141 tcgaagcggc gcaggtaacg cagcaccact tcgcaggcga catcggcgcg gatgctgacc
     1201 aactcgaagt ccataatcgc accgacttgg ttgtcttcgt aggacattgc cgccttgact
     1261 tgggcgcgtt cttcctcatc acgcgtctgt aaggcttcgt aaaccacttg gtgcggcaaa
     1321 tcgtctgcca gttccgccaa ttcgtccgcg tccaaatcgt caactgccgc caacaattcg
     1381 tccttatcca tcgactcgat cagtgtttcg cgcaccgcgt cggatacttc cagcaatact
     1441 tcgccgtcgt cttcgggttt gaccagaagc caaacgacgt tgcgttcgcg cggcggcagc
     1501 gattccaaca ccgccgccac gtcggcaggg tgcagctcgc ccaagaggac ggtcagctcg
     1561 gtcagcttgt cgcgcagcgg cgcgtcttcg agcggtgtgc cgttttcgat ttgttcaaaa
     1621 gcaggttcga ggatttcgca gagggagtgg acacggtcga aatcggcgga agggcgttcg
     1681 gcatggtttt gaccggtttc gccgtcgtgt gtcggaggag tgtgttcgat gctcataaat
     1741 gctccgcccg ccgtgtgcgg ggaggcattc cggcgggatg gttattgtcg tttggaatcg
     1801 gaaggggcgt tcagtaaaaa cgaactggga aggtccataa taaaagcctg acggtacagg
     1861 cgcagggttg gaaacggcac tattctactc ccttttgaaa cggtttacta tttttaacgc
     1921 aaatgccgtc tgaaacgcgg gttccagacg gcatttttac cggcttgtta ccagttgtag
     1981 cctattttga ttcctgcctg tttgtggcgg gcggtgttgc tgtctttgcc gtaagcggca
     2041 tgaagtttgg cgttccagcc tttgtatccg gcgtcgattt gcagggtatg cagtgtgccg
     2101 tgggcggctt gctgcagtaa ggtacggtcg ttgacgctga gggcggaacc gctgttttgg
     2161 cgggtatggt agtaatcgct gctgaaagcg ggggtcagct tggcttgacc cagttcgacg
     2221 gttttatcga gacggatgcc ggcatgccat gtggtttgga tttgcgccgg gctgtttatc
     2281 tctgcgccgt cgagtacgta ccggttgccg ttgctgcggt ttatacggat gccggcatag
     2341 ggtctgaggt tgatgccggt atcgattttg atgccggtgt tgatgcctgc atcccatgcg
     2401 tggcggcgga cggcagcccc gtcataatcg gtaaatcggg tacggctgtt gctgtagcct
     2461 aaatctgccg cggcaaagag tgcgccgttt tcccctttga cgaacagatg tgcgccgttg
     2521 ctgcggtttc gggcggatac gccttcatca aaacggttgt ttgtgcgctc atcggttaaa
     2581 atcgtaccga cactgagacg gtcggtgatg ccggtttgga tgccgatatg tgcatagttg
     2641 gtagtttgtt ggtagggacg gtgtgtgccg ctatggtagt cggtttgttg cgtaccggtt
     2701 tccagccaga tgttttgctg ctgcggatcg gtaaggtggc ggtcgatgcg gcgtcccgcc
     2761 tgttgccggc cggtattgta ggcggcctgt tcggaaacgg cggtgttggc cgaccggctg
     2821 atcagttcgg ccaggttcgg catacggatg ccggcaacgc cggttttcaa actgtggatt
     2881 tgtcgggaca gacggttgag tgcgttcagg taggcgtacc gggctgtttc gacggcttgc
     2941 gcatcgccgc cttgccgtgc tttgtcaagt tcggattcgg ccatttctac acgttctata
     3001 tacgtatcca gttcggtttc gaagagtgtc aggtcgttcg tgtcggcggc tttggcaacc
     3061 tgacggcaga tatcgacact gtatccttgt gcggcacaca ggttttgggc acgcgtcaga
     3121 atgccggtca gcgggtctgt ctgtttcaat tgggcggaca gatattggac ttggctgtcg
     3181 atgcgggcaa gttcggtttg actgttctgc caggcctgta gtgcctgcct ggtcgcgtca
     3241 gagtcatgtt gaacctgtct gctgatgtcg gttgcctgta attggttgta tgcctgttgg
     3301 ttgcgctcat gttccgcacg cgtggcttca atttgaagtt cggcctcttt gagcgggttg
     3361 tacaggctgt atccgttgtt gtttttgcgg aggatgtagc ggtaggcacc caaatcggca
     3421 tagccgtttt ggagggtgaa tcgggcttgg tggctgtgtt tcggattgag gctcacaagt
     3481 gcaagcgatt cggttgtttg aggttcttgt ccggtgtttt tgacgtggat ttggaatgcg
     3541 ccgcggctgt ccccttccag tttgaggggg ggcattttgt tttcggacga tgccggtcag
     3601 gaatcggaat gtgccgaacc cgtcaagtgt gccgttgacg gtcagtgtgt tgaagcggtt
     3661 gttgtgtgta ttattggcga aatcggggtt cagggtaatt tgtgcgccgt caagcgtcag
     3721 tgcgccggtg tggctggact gggaaagtgt ccagttgctg tctgcttcca tgcggactgc
     3781 ggtgtctttg ccggcacgga tgctgccgta caggtgtgct ttgcccaggc ggagctctga
     3841 acggtcgtta agggtaatgt cgccgcgtac ttgcgttgca ggtagtgcac gatagttttc
     3901 ggcttttaaa acggcgttgg gtgtgcagtg ggtgctgccg ctatggtagg agcggtagca
     3961 ttccggtgtt ttgccttggg taaagccgag gtcgatgccg gacagatcgt atgcggttat
     4021 gtcgccgtcc agatgcgcgg tattgcgccc tgccgtcagt cgggcatggt ttcgcagggt
     4081 gaaccgtgca gccttgaagc tgccgtcggt ccattcgttt tcaagaacgg gttcgcgttt
     4141 ggcctggtgg tcgtaggcat ggggtacggg cctgcctgac acaatcatat tgccgccctc
     4201 aatcaagact tccccgttaa ggttcatgcc gccgttgagc aacagcgtgc tgtcggtgcg
     4261 tttgggacga tagtgcaggt tgagtgcgcc gttgtaacgg tattcgggcc tgccgtatac
     4321 ggcggcattt gcgatatggc gggttttttc tatggctgct tcgccggtca tgtccgagaa
     4381 attcccaggc ttgggtgctg taggcattcc atgggaagta ggcgccgggg ttgccgcttt
     4441 ctttgaggcg gtaatcgtcg gtacgcctgc ccctgtgcgg gttggcgtat tcgtataggg
     4501 cgagggcttg tgtggggtgt ttggaccatt cacccattcg atattggttt tgctgtaact
     4561 cggtgcggct ttgcccgttt gcgcgttctc acccaagtat ccgccgaagg taattaggtc
     4621 ggggcgggca ttttcggctt gggcgacttg tttggcggcc tgttgcctgt tgttgccgat
     4681 aaattgccag cttgttgagt ttttcatatt taacgggaaa aattcgcgcg ggttgccgcc
     4741 gggtttgagt atgaagtagt cggtccgacg gttgcggtgc gggttgatgt attcgtaaac
     4801 cgccgcgttg ccttgcggac ggttgcccca ttgcacccac tcgacatgct cgggactgag
     4861 gacggggttg ccggtcagcg tcagtgtcgc ggcttggtca gggttgtggt tgacgatctg
     4921 cgcgccgccg tccgcatggc ggatatgggt aaaggcaagg ttgttgccgt tgaggtcgag
     4981 ccgtccgccc ctgaagccga aatagaggtt ttcgggtttg atttgctggc tgtcggcgag
     5041 gacggccgtg cccctgccgc tggtgatgcc gacttggttg aatgcttgtt tgctgccgtc
     5101 tgaagcagct ttttgggcga gtacgacagt gccttccccg atgctgatgt cgccctggtt
     5161 gatgccttgt ccgttggcga taagcgtgcc tgcgcccagt ttggagagcc ggtcgccttt
     5221 ggggttgctg acttgccaga agacgcgttt gccgtcggct acgataacgc ctgcaccttg
     5281 ccatgtgtgg tttttaccga cgacggtgaa gttgctgtcg aactgcaatg cgcctgcgcc
     5341 ttggttgata ttgtctgcca gcatcagtgt tttgttgtcg aacctgctgg ataggatgag
     5401 cgttttgccg gcatcttcag acggcatgtg cctgctgtcg ttttgtgggg caagcgaagg
     5461 gtttgcaatg ggcagggtga tgcgttcgtt gagcccttgc agggtgctgt cgccattacc
     5521 gttgtcgcgc catatgagtt cgttggtggt cagcccgaca tccagccggg tttcgtattg
     5581 gcggatggtg gaacggatga attcgggttg cgtgacgatg tatttgttga agaaattatc
     5641 gaagccggcg taggtgctga gtacgcccgc aagcacccag cggttttcat gcttgtcgaa
     5701 ggcaaacagg ggggaaccgc tgtctccggc gattgcgtag gtgttaaggg gttggtcggt
     5761 caggctgccg ccgacgagta agccgtggtt ttggaacccc aatactttca gcggcgtgcc
     5821 gccggtcagg tattggtatg ccggagcggt tcgtgtacgc gtgccgtctg ctttgcggac
     5881 ttgttgcgtg cctgagccga gtcgtacaaa gtatgggaag cggtcggtat cgaggtaggc
     5941 attggccttt ggctggccgt ttccaagcaa gggtacgctg ctgagtgcgg caggtgagat
     6001 ttcggtaacc agtttgttga ggcggggaag gtggtagtcg tagtccgggt gcgggttgcg
     6061 tgatacgagg cggtaggtat aggcttgttc ttctggattt tgggtgtcgt tgccgaattg
     6121 tatggaaccg tagccgacgt tgtgtttgac actgttgacg tattgggggt gaaccagggt
     6181 ggcgatggcg gtttggcggt tgctgacgcg gaagtcgggc atggggatgc cgttgagaac
     6241 cctgcctaat attttgcctt gcttgtcttg gatggaaata ttgcttgcac ctacggcgaa
     6301 cgcgccttta ttttcggcaa agtcgcgaaa atattggtaa tcgacatcgt tgcggacaat
     6361 ggatgagtaa gcaggggaaa cggcaaaaag accgagggtg gacaatacgg aacaaaatgg
     6421 ggtggtgtgt gtgaagcgca tgatgattat ccgtgtaata gagaaaatca acgattcttg
     6481 cggacaagaa tcaccgatcc cgttttgtgc tggatttata tagtggatta acaaaaacca
     6541 gtacagcgtt gcctcgcctt gccgtactat ctgtattgtc tgcggcttcg tcgccttgtc
     6601 ctgatttaaa ttcaatccac tatattatat attttgtttt tgtttgatta taaggcaacg
     6661 gtgtcctttc gtctattggc gatgattgta tgattttatt taagatttta ataaaaatcc
     6721 taggaatggc tgcgttttcc cgttgtttta tttcgtccgg tattggtttt taagttcacg
     6781 cagcgcgaca aaggtatcga ggctgctgtt taacgttttc ccgctcaatg cctcggcgcg
     6841 gtctctgacg tgcggcaggt cgacgcgcaa aaacggattg acacggcgtt cgtgcgcgag
     6901 ggtaacgggc agggtaggcg tatgcgccgc cgctttcagt gccgtctgaa tgtcggcgtt
     6961 gtccggctcg atatgggcgg caaaatccag gttggcggcg gtgtattcgt gcgccggata
     7021 gaacagggtg ttttcaggca ggcggttgaa gcgttggaag ctgtcgtaaa gctgttcgat
     7081 tgtgccggta aacacgcgtc cgcagccggc ggaaaaaagg gtgtcgccgc aaaagacgtg
     7141 tatgccgtct gaagtttcga gaaggtagct ggtgtggcgg tctgtgtggc cgggtgttgc
     7201 ccaaacggta acctgtccgt cgccgaaggt gaattgggtg ccggcggtta cggtgtgggt
     7261 tgctgcttcg atgtcggatt cgccgtaaac gggcgattcc atgtagccgc gccagagtgc
     7321 cgccgcaccg ccctcgtggt cgggatgggg gtgagttacc catgtttggg caagcatgag
     7381 gcggttgcgg acgaggaatt ccaagacggg cgagggttcg gaagggtcga cgcagacggc
     7441 atggttgccg tgctgtatca tccagatgta gttgtcggtc aaggctttga cgggggtgat
     7501 tttcatgagt gttcctcggt ggacggatac cgtttaaagg atggtatgcc ggaacgccgt
     7561 ctgaaatatt cttcaggcgg cattggtttg agtagggcgg ttagcccaag gctttacgtg
     7621 cgccggcaaa gaggcggtac cagccggaca gttccgtcca gccttccggt ttccagctca
     7681 tttgcgcggc acggtacacg cgttcggggt ggggcatcat aatggtcacg cggccgtcgg
     7741 cgttggtcac accggcgatg ccttgcggcg agccgttagg gttgagcgga taggtttggg
     7801 taacctggtt ttgtccgtcc acatattgca gcgcgatgcc caaatcagca gaaatgttgc
     7861 cgccgtgaag cgcgaagtcg gcgcggcctt cgccgtggct gacgacgacg ggcaggctgg
     7921 agccttgcat ttcgttcaga atcagcgacg cggatttggg gacgtgaacc atactcaggc
     7981 gcgcttcgaa ctgttcgctc aagttacgtt tgaacttcgg ccatgcctct gcacccggga
     8041 taatctcggc gaggttgctg accatttggc agccgttgca cacgcccaat gtcagcgtgt
     8101 ccgggtcggc gaagaaggcg gcaaactggt cgcgcagagc agggtggaac aggatggatt
     8161 tcgcccagcc ttcgcccgcg ccgagtacgt cgccgtagct gaagccgccg cacgccgcca
     8221 gcattttgaa gtcggcaagg cggaagcggc ctgccatcag gtcggacata tgcacgtcgt
     8281 aggcatcgaa tccggcacgg gtgaacgcgg cggccatttc gatttgcccg ttcacgccct
     8341 gttcgcgcag gatggcgatt ttgggtttcg cgccgctgtt gataaacggc gcggcgatgt
     8401 cttcgttcac gtcgaatttc aggttggcaa acaatgcgct gcggtcgttg tcgccaatca
     8461 gggcaaactc gctgtcggcg caggcagggt tgtcgcgcag gcgttggatt tgatggctgg
     8521 tttcttgcca ggtttgttgc agtttgatta ggttgtcaga aataaggtgc gtttgcccgt
     8581 cgcggatgat taacgtgttc tcatcagtta acgtaccgat ttcaaagaca ttatgatgca
     8641 gctgttgttg atagaataaa ttgataatat cggcaacatc ttgttttcta acttggataa
     8701 cagcacccaa ctcttcatta aataatgtgc gggcaatggt ttcttgccat tcagccaacg
     8761 cttttacctc ttcagtccgc aatgattgag acagagcggt atggttggta ataaatgttt
     8821 gtgcaagcaa taaatttaaa tctatatcca agccgcaccg ccctgcaaac gccatttcta
     8881 ccaaaacggc aaacaagccg ccgtcgctgc ggtcgtggta tgccaagagt ttgtcttcgg
     8941 cgacaagctg ttgaatcacg ttgtaaaacg ctttcagacg gcctgtgtcg tccaaatcgg
     9001 gcgcgtcgcc gctcatgttg ttgtatacct gaccgaatgc agagccgccc atacgcgctt
     9061 tgccgaagcc caaatcgaca aacagcaata cgctgtcctc gacgtttttc aattcgggcg
     9121 taacggtttt gcgtacgtct tgaaccggcg cgaacgcgga gataatcagg cttaacggcg
     9181 aaacgacgga ttttttctct tcgccatcct gccatacggt tttcatcgac aggctgtctt
     9241 tgcccacggg aatgctcaaa tccaatgcct gacaggcttt ggaaaccgct tcgacggtgc
     9301 ggtagagttt ttcgtcttcg ccttcgttgc cgcacgccgc catccagttg gcggagagtt
     9361 tgatattgcc gatgtcgccg atgttgaccg ccgcgatgtt ggtgatggct tcgccgacgc
     9421 acattctgcc cgaggcgggc gcgtcaaaca gggcaacggt cggtttttcg cccatagaca
     9481 ttgcttcgcc gcgataggta ttgaagccca tcatggtaac ggcgcagtcg gctacggggg
     9541 tttggtattt gccgaccatt tggtcgcggt gggtcatgcc gccgacgctg cggtcgccga
     9601 tggtaatcag gaagtttttg gcggctacgg caggcaggcg caaaacgcgg taggcggctt
     9661 cggtgatgtc gatatcgtcg gcgtgaaacg gttttttgga cggcgtaacc gttttgtcgg
     9721 tacgcgtggt tttgggcggt ttgccgagca agacgttcag cggcaaatcg acggggttgt
     9781 tggcgaacaa atcgtcgcgc actttcaaat ggccgtcgtc ggtcgccgtg ccgaccacgg
     9841 caaacgggca gcgttcgcgt tcgcagatgg cgcggaaggt gtccaaatct ttttccaaaa
     9901 tcgacaacac ataacgctct tgcgattcgt tacaccagat ttgcaaaggc gttaaaccgt
     9961 gttcttccaa cggcacttcg cgcagcttga ataccgcgcc gcgtccggca tcgttgacga
    10021 gttcggggaa ggcgttggac aaaccgcccg cgcccacgtc gtggatggag ataatcgggt
    10081 ttttgccgcc gagctgccag cagcggtcga tgacttcctg cgcgcggcgt tcgatttcgg
    10141 ggttgccgcg ttgtacggaa ttgaagtcta aagacgcgtc gttcgtgccg gtatccatcg
    10201 aagaagccgc gccgccgccc aagccgataa gcatgcccgg gccgcccagt tgaatcagca
    10261 atgcgccttc ggggatttcg tctttatgcg tctgctgcgc ctgaatgctg cccagaccgc
    10321 cggcaatcat aatcggtttg tggtagccgc gaacctgacc gtcaaatttt tcttcaaagg
    10381 tgcggaagta gcccaagagg ttggggcggc cgaattcgtt gttgaacgcc gcgccgccga
    10441 tggggccttc aatcataatg tccagcggcg aggaaatatg ttccggcttg ccgtaatctt
    10501 gttcccacgg ctgtttgagg caggggatgt tgaggttgga cacggtaaag ccggtcaagc
    10561 ctgctttcgg acgcgaacct ttgcccgtcg caccttcgtc gcggatttcg ccgcccgcgc
    10621 ccgttgccgc gcccgcaaac ggcgcgatgg cggtcgggtg gttgtgcgtt tccactttca
    10681 tgatgatatg cgtgtcttcc tcgtggaaac ggtagccttg gttttccgcc gcatccggat
    10741 agaaacgctc gattttcgcg ccttcgatta cggacgaatt gtctttataa gcgacgaccg
    10801 taccttcggg atgcgcgttg tgcgtgtcgc gtatcatgcc gaacagggat ttcggttgtt
    10861 tttcgccgtt gaggatgaaa tcggcgttga agattttgtg gcggcagtgt tcgctgtttg
    10921 cctgcgcgaa catcatcaat tcaacatcgg acggattgcg ctgcaaagcc tgatagtttt
    10981 caaccagata atcgatttcg tcggcggaga gtgccaaacc catttcggta ttggctttga
    11041 ccaaagcctc tttaccgccg cctaaaacat caacggtgga aaaggtttcg gattcgagat
    11101 ggtggaataa tttggatgcc gtctgaaaat cgggcagcac ggattcggtc atgcggtcgt
    11161 gcagcaacgc cgcccattgc tgtttctcat cgtcgttcag acgaccttcc agccacaccg
    11221 ccatgccgcg ctcgatgcgt tcgatgcctg ccaaaccgca gttttccgcg atattggtcg
    11281 ccttggaagc ccacggcgaa atcgtaccca aacggggcgt taccaaaaac aaatgcaagc
    11341 cctcgcgcgc ttttggcgtt tgttcaacgc tttgcgccgc caacaaggct tgcagttttt
    11401 cgacagtcgc ggcatccaat gctttctcgc tgccggcgaa ataccaaaat tcgctgctta
    11461 atttgacttc gggcagtccg agtgcggcgg ctttttgcaa gagtttttcg acacggaaat
    11521 cggaaagggc ggtaacgccg cgcaagggca aaacgacaga catggattcg gctctcaaat
    11581 gcggttggaa aatctgtcat tatacgcgca acggggctgt attgcttggg tattttggct
    11641 taaaatgttg acaatcttca gacggcatac agttggggaa aatacaggtg ccgtctgaag
    11701 atattaagga ataagggttt tatctgtttt tctgattttt acccaaagcc gggtatgcgg
    11761 ataattgcag gaagtagagg ggcgggcaac tgcttccgta tgtttttaga tcaaatgccg
    11821 tcgtttccgt acggttagga atatggaagt ttggatttac agaaaccttt aaatattgct
    11881 gaaatgcaga gatcgggatt cccgcctgcg cgggaatgac ggtttccggc aaatcgccgc
    11941 atcgccgggc gaacagtacc gtttatcgtt tgccggcggt ttctgctata tttgtcgttt
    12001 gtttacgtta attaatggag aatgtgatga aaaaaatcga ggcgattgtc aaaccgttca
    12061 aactcgacga cgtgcgcgag gcgttgacgg aaatcggcat tacgggtatg accgtcagcg
    12121 aggtcaaagg gttcggcagg cagaaggggc atacggagat ttaccggggc gcggaatacg
    12181 ctgtcgattt cctgcccaag gtcaaaatcg agctggtgtt ggcggatgac gctgtggaac
    12241 gcgcgattga cgtgattgtc gaggtggcgc gttcgggaaa aatcggcgac ggcaagattt
    12301 ttgtgctgcc ggtcgaggag gcaatccgta tccgcacggg cgaacgtgcg gacgcggcgg
    12361 tttgagcgtt tggaatggaa atgccgtctg aaaggttttc agacggcatt ttctttttga
    12421 gcttcagata tggtggacga tgagtttttt gctttggtgg tcgaggacgg acacatccat
    12481 atcgaacagg gttttgatgt ttgcggcggt gaaaatgtcg tcgggcttgc cctgcatggc
    12541 gacttggccg tttttcatgg cgacgacgtg gtcggcgtag gcggcggcct ggttgatgtc
    12601 gtgtaatacg acgacggtgg tgcgcttgtg ttcgtcggtc agccggcgca ggatttgcat
    12661 gagggagcgg gcatgataca tgtcgaggtt gttcagcggt tcgtccagca aaacgtagtc
    12721 ggtgcgctgg cagaacacca tggcaatcat ggcgcgttgg cgttgtccgc cggaaagctc
    12781 ggtcaggtag cggtcggcga agtcttgcag gtggaattct tcgattgcac cgttaacgat
    12841 acggcggcat tcggcagtcg gtctgccttg atggtaggga tagcggccga acatgagcag
    12901 gtcgcgcacg gtgatgcggc tcatgatgct gttttcttgg gtgaggatgg acagggtttt
    12961 ggcgagttcg gcggtggggg tgcaggcaag gtttttgccg cggtaggcga tgctgccgct
    13021 ttcaagcggg cgcagccgcg ccataaagga aaacagggtg gatttgcccg caccgttggg
    13081 gccgacgagg gcggtaatgc cgccttcggg gatgtcgagg ctgacgttgt cgaggatggg
    13141 gcgtgtgccg atgcggtagc tgacgttgcg gatggtaatc atggcggatg cttgtcgggc
    13201 gtaggtttgt tgttgtttgg cgggggttcg gacggcattt gaaattcgga atgccgtctg
    13261 aacgctcaca cttcgcggac gatgacgaac tgcgggtcgt ccgccgattc ggtcaaaaat
    13321 gctttaacgc gtacctgcca gagttgtccg aagcgttcga gttct
//
LOCUS       DAWHXS010000057        13287 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWHXS010000057 DAWHXS010000000
VERSION     DAWHXS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.057"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(84..737)
                     /locus_tag="WIE22_001262"
     CDS             complement(84..737)
                     /locus_tag="WIE22_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ4127341.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
     gene            complement(769..1281)
                     /gene="coaD"
                     /locus_tag="WIE22_001263"
     CDS             complement(769..1281)
                     /gene="coaD"
                     /locus_tag="WIE22_001263"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ4127342.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            complement(1405..2088)
                     /locus_tag="WIE22_001264"
     CDS             complement(1405..2088)
                     /locus_tag="WIE22_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ4127343.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPASAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            complement(2245..2511)
                     /locus_tag="WIE22_001265"
     CDS             complement(2245..2511)
                     /locus_tag="WIE22_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ4127344.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(2584..3054)
                     /gene="rlmH"
                     /locus_tag="WIE22_001266"
     CDS             complement(2584..3054)
                     /gene="rlmH"
                     /locus_tag="WIE22_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ4127345.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            complement(3108..3494)
                     /gene="rsfS"
                     /locus_tag="WIE22_001267"
     CDS             complement(3108..3494)
                     /gene="rsfS"
                     /locus_tag="WIE22_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ4127346.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(3553..4146)
                     /gene="nadD"
                     /locus_tag="WIE22_001268"
     CDS             complement(3553..4146)
                     /gene="nadD"
                     /locus_tag="WIE22_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ4127347.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            complement(4143..4742)
                     /locus_tag="WIE22_001269"
     CDS             complement(4143..4742)
                     /locus_tag="WIE22_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ4127348.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(4771..6312)
                     /locus_tag="WIE22_001270"
     CDS             complement(4771..6312)
                     /locus_tag="WIE22_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ4127349.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
     gene            6705..8090
                     /locus_tag="WIE22_001271"
     CDS             6705..8090
                     /locus_tag="WIE22_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ4127350.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            8110..8628
                     /locus_tag="WIE22_001272"
     CDS             8110..8628
                     /locus_tag="WIE22_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ4127351.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(8967..9884)
                     /gene="thrB"
                     /locus_tag="WIE22_001273"
     CDS             complement(8967..9884)
                     /gene="thrB"
                     /locus_tag="WIE22_001273"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ4127352.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(9921..10649)
                     /gene="ubiG"
                     /locus_tag="WIE22_001274"
     CDS             complement(9921..10649)
                     /gene="ubiG"
                     /locus_tag="WIE22_001274"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ4127353.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            10801..12042
                     /locus_tag="WIE22_001275"
     CDS             10801..12042
                     /locus_tag="WIE22_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ4127354.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRQKFGAGKTYKVYGGLWLMVWVFLF
                     GIINIAAQVLSQMELVPVFKG"
     gene            12192..13247
                     /locus_tag="WIE22_001276"
     CDS             12192..13247
                     /locus_tag="WIE22_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ4127355.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMQRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADFFEHTDL
                     PVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDILASV"
BASE COUNT         3064 a         4005 c         3344 g         2874 t
ORIGIN      
        1 tgaagagatt tgcagtcaac cgttttcttg aaaaattttt ataaaaatat ttatcatatt
       61 gtttataaat taatttatat ttattaacct gcttttgcca tataaaattc ttccagcgca
      121 tgagagatat tttcagtctg ccattcgttt tttaagggtg caacaatttc gatttgtcgg
      181 ttttggtagg caaattgtat tttccatgca tacagaaaca tggtttcgga ttctgttccg
      241 ccgtataagc tgtcgcccag aatcggactg cccaaacttt tcatcgccac tctcaattgg
      301 tgcgttttgc ccgtatgcgg ttctaggatg aacagccgca gtttttcggc gatactgatg
      361 ctgtggaatc gggtaacggc gatattttct gtattgcgcg tcaacttcca cattccacat
      421 ctggattttt ccattccgcc tttaatccaa ccctgctttt tggacggctt gcggtcggac
      481 agtgccaaat aggttttttt gatgcttttg ccggcaaact gtccgctaag ggcggacgcg
      541 ctttctttgt tgagggcaaa cagtaaaatg ccgctggtct gtttgtccaa tcggtgcagc
      601 agccacacac gctctacgcc caactgtatg gcgagtgttc gggccagtcc ggtctcgccg
      661 ctgtcttggt ggacggatat gccgcccggt ttgttgatgg cgacgaagtc ttgatggcgg
      721 aacaaaattt ccaacatatc catatatgcc ttgcaaaaat agaagggttc aattttcgtg
      781 ttgatgttcg gcaaggattt tttcgtacac agcttgcggc acgtagcggt ggatcgtttc
      841 cgtccagcct tccggcccga ccagtccttt gaccatagtg gacgacactt cggcgatttc
      901 gcgcggcggc atgaggaata cggtggatat ttcgggggcg aggtcgctgt tgatatggcg
      961 catggaacgt tcgtattcgt aatccgaagc agaacggatg ccgcgcacga tgaatcctgc
     1021 atctacctca cgggcgtaat gcaccaaaaa tcggttttca aatacatcgg ttctgacgtt
     1081 gggaaacatt ttagtaatat cgcacaacat atcctgcctt tcagcgacgg tataggtgct
     1141 gcgtttgtcg gggttaatgc cgatggcgac gatgagttcg tcaaacatag attgcgcctg
     1201 ccgtatcatc cacagatgcc ccaatgtggg cggatcgaaa ctgccggcat aaacggcgcg
     1261 gcgcggtgta ttcggtaaca tttgattcct cccggcttca tagtcggctg tgtgttggtg
     1321 cgtgtgcatc cgtattgtat gcccaaagta aaatgccgtc tgaagcattt tcagacggca
     1381 tagtcggacg gcgttttacc ggcatcaatc ctcgccgttt aaagacaaca ggatgttcag
     1441 caggctgctg aagatgttgt aaagcgagat aaacagtgtc agtgccgcgc tgatgtggct
     1501 gtcttcgccg ccgtcgatga cggtgcgtac ctgccacata atcattaagg aactgaacaa
     1561 gacaaaaccg gcggaaatgg tcagggcgag tgcgggaata cccaaaaaca gattggcaac
     1621 cacggcgacc atcagaatga ccgcacctac ggtcaggaag cgtccgagcg cgttcatatc
     1681 gagccgggtt cggcgcgcca aggcggacat cgttaaaaag acggcggcgg tcatcgcggc
     1741 ggcaatgccg acgattttcg caccgtcggc aatatggagc gcgtattgca gcacggggcc
     1801 gatcaatacg cccataccga atgtgaatac catcagcagg gtaacgccgg tattgctgta
     1861 acggtttttc tcgatgaagt ggatcatacc gtagaaaaac gccaacacga cggcaaaccc
     1921 tatccagcgc gaaccgaagg cggcgtaaaa attgaaaccg gcattggcgg caagtgccgc
     1981 gcctgcggaa gccggaataa atgaaaatcc gagcaggcgg taggttttct gcaggacggt
     2041 gtttttagaa accgtatgcg cggtgtagtc gtaaacgtcg tgttgcatat catctgctcc
     2101 tgaaagcgcg gttgggaata atgggggatt ttaacattgc ccaatgtcaa aatttgtccg
     2161 gttgcgtgaa gataaagttg tccggcgtat tttaaaggcc gtctgaagca gtttcggaca
     2221 gcctgtgttc aaaacggaaa accgttattg cggaacgtat ccctgaacgg catccgcgcc
     2281 gtcgccgaag aaatactgct ccatctgctg agccaggtat tcgcgcgcgc gcggatcggc
     2341 gaggcttaaa cggttttcgt taatcagcat cgtttggtgg cgcgtccacg ccgcccacgc
     2401 ttcttgcgat acgttttcaa aaatgcgctt gcccaattcg ttgggaagcg gcggaaattt
     2461 catgccttcg gcttctttgt tgagcttgac gcagaatacc atgcgtgcca tagcggattc
     2521 ctttgctgtg ttcagaaata acggggtgat tttaaccgat tagggatacg gacaaaagcc
     2581 ttcttattcc cgatgatagg gatggttgtg caggatggaa acggcgcggt agagctgctc
     2641 ggtcagaaag acgcgcacca tgccgtgcgg cagggtgagg ctggacaggc gcatcatcat
     2701 gcgggcttgt tgtttgaggc ggtcggtcat gccgtccgcg ccgccgatga cgaagcagac
     2761 gtgttcgccg ttttgccgcc agcttttgag gtgttccgcc agctcgacgg aggtcggcgc
     2821 tttgccgcgt tcgtcaagaa cgacgaggaa cgcgccttgc ggaacggctt cgaggatgcg
     2881 tttttcttcc gccgccatac cttgggcggc attcacgccc gcgccgcgtt tttcgggttt
     2941 gatttctttg agtgcgtagg cgacgtcgcg tccgaagcgt ttggcgtatt cggcgacggc
     3001 ctcatcaacc cagcgcggca ttttggtgcc gactgccaaa acggtaatgt tcaatgcttt
     3061 ctcccttaca ggaaaatgcc gtctgaacat tcagacggca tcgggaatca gtctgccgcg
     3121 tgccacggct tctgcattcc ggcgtgaaaa ctcggtttct cgccgcccca aagggtgtcg
     3181 atatcataga agtcgcgcac ggcggggagc ataacgtgga cgacgaggtc tcctgcatca
     3241 acgagcgtcc attcgccgct gtctccttcg gtactgagga tttcaaaacc ggcttctttc
     3301 aaatcgacgg caacgttgtt ggccagtgct ttgacttggc gcgtactgtc gccgctggcg
     3361 ataatcattc tggcaaacag cgaagttttg tcttgcgttt cgagaacgga aatgtctttg
     3421 gctttgatgt cttcgagggc gttgacggcg accccgacca ttttttgcag gtcttgcagt
     3481 tcttgttcgt tcattatttt cctaacggga tgttttcaga cggcattata gccgtttctt
     3541 actgatttga ctttattttt catacaaacc gtgttcgcgg atgtagcgtg cggcggcagg
     3601 tgggatgccg tctgaaacac cggcgcggcg gatttccgtt gacgacacat tatgcatcgg
     3661 ggcggacaag atgcggacgc tgccgtcctg aagggacttg cccagccacg cgtgcagttc
     3721 gcgcggggtt tggtgcaggc tgtcgccctg ccgcatggcg acggcgatat tggtttcgcg
     3781 cacgagcatc tgccattttt tccatgtgtg cagcttcatc aggctgtcgc tgcccatcag
     3841 ccaccagagt tgcgcggatg ggaactgctg gcggaagatt tggacggtat caaaagtata
     3901 ggttgcacct tctcggacga tgtcgcaatc gctgacggca aaacgcgcgt cttctgccgt
     3961 cgccagttcg accatggcaa ggcggtcggc ggcggaagcg gaggctgcgt ctttgtgata
     4021 cgggccgcct gccggcagga aaacaaccgc gtccaagccg atttcgtcgg caaaggcgcg
     4081 ggcgatgtgg agatgcccgt tgtgtatcgg gtcgaaagtg ccgccgaaca gtccgatttt
     4141 cttcatgatg tttccattcc ttccgataag tccatgccgt ctgaaacact gccgtccaca
     4201 accagcgtca gtttgccggt aacgggatgg atgaccagtc cgtgaacggc gatatggcgc
     4261 ggcatcagcg gatggttacg gataaggtcc accgtgtggc gcacgctgtc ttcgacgttg
     4321 tcgaaaccgg tcagccagcc gtcgaggtcg ataccggcat aacgcagggt ttcgatacgg
     4381 tcttcgggaa tctggctttc ccggacgcgc ccgaggaatt cttcggcatt cagcccctgc
     4441 ataccgcaat cgtgatgggc gatgaccata atctctctga ccttcaattc aaacacggca
     4501 accaaaagac tccgcatcac cgaaccccac gggtgcgtaa ccagcgcgcc ggcattttta
     4561 atcagcttgg catcgccgtt tttcaaaccc aacgcgtcgg gcagcagccc gataatccgc
     4621 gcatccatac aggacaaaac tgccagcccg cgttcggggt atttgtcggt aaagtatttt
     4681 tcatattcgc ccgactcgac aaactgccgg ttatgggcaa ggatgttatc caactcgctc
     4741 attttgccgt cctctgaaaa agggttcaca ttataacgtt tccgtctgtt ttccgccttc
     4801 gccgttgtcc aacagcagga aaatgcccag cgcgaacgcc gacaacagca atgtcaacac
     4861 cattgcccgc gcataattat cctcacccgc acgccccaaa taggcataaa tcaaagtcgt
     4921 cagcgtctgc cactcgggac gcgacaggaa caaggttgcc gcaaattcgc ccacgcaggt
     4981 tgccgccgcc aaagtcagac cgcgccgcaa cgccggtttc aagaggggga acgtgatgcg
     5041 gcatgccgtc tgaaagccgt ttgcacccaa acccgccgcc gccctgccgt aatccggcgg
     5101 cagtgcatcc caggctgata aaacatcttt tgccacaaac ggatacgcca gcagcgcata
     5161 catcgccagc agcagcggca acgaagccgt ccactgcgga taaagcagca gcacgcccgc
     5221 cgaaacacaa accggcgaca ccataaacgg caaaaacatc agcccgcgca tccacgccga
     5281 ccgccgcgcc gccgccgcat acaccacacc caaaaccgcc gccgcataca ccgccgccgc
     5341 cgagaagcgc aaagtattcc acaccgcctg ccacgtttca ctttccatta acacacgcca
     5401 cgattcgccg gccgaccacg ctttcacaac aattgccaac aaaggaaaca ggcagcacac
     5461 agacaacacc gccgccgcaa acgccagcag cacatattcc ccgaccgact gcggcggcga
     5521 cggcatcaca ggggaaaccg ccttatccga aaccgcgcgc ctgccgaacc acgcatacag
     5581 caaccctgcc gccgccgtta cccccaacac cagccacacc agcaccgaag caaccgccat
     5641 atcgagttcg aacatgacca actggtaaat ttccacttcg accgtggcat aacggctgcc
     5701 gcccagcagc aatgccagcc cgaaccccga aaaacaatac aggaagacaa ggcacacgcc
     5761 gccggcaagc cacgggcgca aaacgggcat ttcaatgtcc aaaaaccgcc gccacgcccc
     5821 cgcgcccaac gtccgtgccg tctgaagccg tgccgcaggc acttgcacaa acccctgata
     5881 cgccgccctg accaacacag gaaggttgaa aaacacattg ccgtacaaca acagatacgg
     5941 cgtatcctgc ctgccgcgcc acaacagccc gtccgccccg aacagggcca gcacgcccac
     6001 gcccgccacc aacgtgggca tcacaaaagg cagcatcagc aggcgcagca ctaaagcccg
     6061 ccccggaaac gccagccgcg ccagcaccca cgcgacaggc acgcccaaag gcagcaccag
     6121 cacacaggtt gccgctgcct gaaataccgt ccacgccaaa cgtttgagca tataggcatc
     6181 cgacagcacc gcgcgccacg ccaaaccgtc atacgccgcc accgcccaca aaggcgcaac
     6241 gaccattacc gccaaaaaag ccgaaggcag cagggcaaaa gcaccccata ccacccaacg
     6301 ccgtccatcc atcgccttcc ccacttgaaa cactgatgtt gcgattgtac ccaaaagccc
     6361 ccacataccg tatatttcaa tccgactaca taccgtatcc gccttcctcc cgccgtctga
     6421 aatatagtgg attaacaaaa atcaggacaa ggcgacgaag ctgcagacag tacaaatagt
     6481 acggaaccga ttcactcggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     6541 gaggcaacgc cgtactggtt tttgttaatc cactataaat cgttcaaata aacaggaata
     6601 taacttcaga caaacaactt accgcccgat ttgtgctatc gttttcgcac aacttaaaaa
     6661 aacctgacaa ttttgtactt ttattacaga gaaaggcttt acaaatggac ggctggacac
     6721 agacgctgtc cgcgcaaacc ctgttgggca tttcggcggc ggcaatcatc ctcattctga
     6781 ttttaatcgt caaattccgc atccacgcgc tgctgacact ggtcatcgtc agcctgctga
     6841 cggctttggc aaccggtttg cccacaggca gcattgtcaa cgacgtactg gtcaaaaact
     6901 tcggcggcac gctcggcggc gtggcgcttc tggtcggcct gggcgcgatg ctcggacgtt
     6961 tggtcgaaac atccggcggc gcacagtcgc tggcggacgc gctgatccgg atgttcggcg
     7021 aaaaacgcgc accgttcgcg ctgggcgttg cctcgctgat tttcggcttc ccgattttct
     7081 tcgatgccgg actaatcgtc atgctgccca tcgtgttcgc caccgcacgg cgcatgaaac
     7141 aggacgtact gcccttcgcg cttgcctcca tcggcgcatt ttccgtcatg cacgtcttcc
     7201 tgccgcccca tccgggcccg attgccgctt ccgaatttta cggcgcgaac atcggccaag
     7261 ttttgatttt gggtctgccg accgccttca tcacatggta tttcagcggc tatatgctcg
     7321 gcaaagtgtt ggggcgcacc atccatgttc ccgttcccga actgctcagc ggcggcacgc
     7381 aagacaacga cctgccgaaa gaacctgcca aagcaggaac ggtcgtcgcc atcatgctga
     7441 ttcccatgct gctgattttc ctgaataccg gcgtatcggc cctcatcagc gaaaaactcg
     7501 taagtgcgga cgaaacctgg gttcagacgg caaaaataat cggttcgaca ccgatcgccc
     7561 ttctgatttc cgtattggtc gcactgtttg tcttgggacg caaacgcggc gaaagcggca
     7621 gcgcgttgga aaaaaccgtg gacggcgcac tcgcccccgt ctgttccgtg attctgatta
     7681 ccggcgcggg cggtatgttc ggcggcgttt tgcgcgcttc cggcatcggc aaggcactcg
     7741 ccgacagcat ggcggatttg ggcattcccg tccttttggg ctgtttcctt gtcgccttgg
     7801 cactgcgtat cgcgcaaggt tcggcaaccg tcgccctgac caccgccgcc gcgctgatgg
     7861 ctcctgccgt tgccgccgcc ggctttaccg actggcagct cgcctgtatc gtattggcaa
     7921 cggcggcagg ttcggtcggt tgcagccact tcaacgactc cggcttctgg ctggtcggcc
     7981 gcctcttgga catggacgta ccgaccacgc tgaaaacctg gacggtcaac caaaccctca
     8041 tcgcactcat cggctttgcc ttgtccgcac tgctgttcgc catcgtctga cagacggaaa
     8101 ggatagtaaa tgactacgca ttttgtcgtt atgggcgtat gcggctgcgg caagaccacc
     8161 gccgcgctgt ccctgcagaa acacctcggt caatgtccct atgccgaagg cgacgagttc
     8221 cacacccaag ccaaccgcga caagatgggc gcgggtattc cgctgaccga tgaagaccgc
     8281 tatccgtggt tgggcaatct gcgcgactgg atgacgcaac aggcgcaaaa cggtgcgaac
     8341 cacaccatcg taacctgttc cgccctcaaa cgcggctacc gcgacattct gcgcggagcc
     8401 gaaggcaaag ctgccttcat ccacctcagt ccgccgcaag acatcaacct cgagcgcatg
     8461 atgtcgcgca aaggacatta catgaaagca gggatgctcg attcgcaact ggaaatcctc
     8521 gaggaactgg gcgaaggcga atacggggtc aaaatcgcca accccggcac gcccgaagcg
     8581 gtcgaagccg atattctgaa ctgggttgcc tcggaaaacc tgctttgaag caatatgccg
     8641 tctgaagccc gacacaggat gggtttcaga cggcataaac atcgggaaca gaatggatta
     8701 cattgattta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga
     8761 gaacgattct ctaaggtgct gaagcaccga gtgaatcggt tccgtactat ctgtactgtc
     8821 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataaaatgga aaatacccgg
     8881 ctatcgtctc attttcgttt taatcagcca taaaaatgcc gtctgaaacc ctttcagacg
     8941 gcatttctgt caaacgccgg acgcactcaa tccaaactca acagcaggtt gcggaacgcg
     9001 ttcgggtctt tgataaacgt catctcgccc gcctgcggaa aatgaaagtc caacaggcgc
     9061 gacacccaaa aacggatgca gccggcacgt tgggcggtcg ggaaatacgc cttttcttcg
     9121 gcactcaagg ggcgcacgcc ctcataaccg ccgataaaca cctttttcaa cgcctcatcc
     9181 aacttattgt ccgccgtcct tgcccaatcg ttgaccgcaa tcgccaagtc atacataaaa
     9241 ttgccccggc aggcataata gaaatcgatg aaacccgata cctgaccgcc gtcaagcaac
     9301 acattgtctt taaacaaatc ggcatggatg atgcccgaag gcagatgatt gccgagattg
     9361 tcctttaacg catcgatttc ggaacacagc agcgcggcat cgtcttgcga caggacgggc
     9421 agcagccggg tgcacgcctc cgtccaccac gcatcgtaac gcgggttttc catttccaaa
     9481 gggaaatcgg cggcggcaag gtgcattttg gccagcatcg caccggtatg gaaacactgc
     9541 tccgccgtcg gcagcgcggt atccgaacct ttcaggcagg caaccaaaca agccggcttg
     9601 cccgccaaaa cggaatcaag ccggccgtct ttgcgcgcaa ccggcgcggc aaccgccacg
     9661 cccttcatac tcaaatgccg gttaagctcc agaaaaaacg gcagctcttc ctgtttcaac
     9721 acttcaaaca cggtcagcac ataacgtccc gaagtcgtcg tcagaaaata attgctgttg
     9781 gtaatcccct gcgcgatgcc ctgcagggaa acaaattccc ccaaatcgta accgctcagg
     9841 aagccgcgca tttcatcatc ggaaacactg gtatagacag acatatcaac tcacttgctc
     9901 aaaaacgggc aaacccgccg tcagaacgcc ggacggcagg caaacatata attcacatcg
     9961 gtcgaatcgc acagggcata agtttgcgac aacacatggt aagtcatacc cttcgtatcc
    10021 gccacatcca agcctgcctg acggcacatt cgcgccagct cggcaggtgc gatgaatttt
    10081 ttccagtcgt gcgtgccttt ggggacaaac ttcaacagat attccgccgc cacaatcaga
    10141 tgcaggtacg atttcgggtt tttattgatg gtggaaaaaa acaccatgcc gtccggcttg
    10201 accagcttgg cacaagcacg cacgatggcg gcgggatcgg ggacgtgttc catcatttcc
    10261 atgcacgtta ccacatcgaa cgagtgcggt tccgcctcgg caaggtcttc cacgcggata
    10321 cattcgtatt cgatatcggc gacattgttc aaagccgcgt gcaggcgggc ggtttccaac
    10381 gactgctccg ccatgtcgat gccctttaca aacgccgcgc cgcgccgtgc catactttcc
    10441 gccaagatgc cgccgccgca gcccacgtcc aaaacccgtt tgccgcacaa atccgcgtgc
    10501 ccgtcgatat aatccagccg cagcggattg atgtcgtgca aggttttgaa ctcgcccgac
    10561 ttgtcccacc atttgtcggc aatccggctg aatttggcga tttccccctc atcgacatta
    10621 tattttttgt cggacatttt ccctcccatc tgacgaaccg cccactccaa aacccaagat
    10681 tatactatgg aacacaatgc cgtctgaaag ccttttcggg cggcgcggcg caaatgcctt
    10741 acaaatcctt acactttacg gcataatggc ggctcgcttt ttctggcaga aagacaaaat
    10801 atgcccacca aaaccccttc actgttcggc ggcgcgatga ttatcgccgg cacggtcatc
    10861 ggcgcaggta tgctcgccaa cccgaccgcc acatccggcg tatggtttac cggctcgctg
    10921 gccgtgttgc tgtacacctg gttttctatg ctttccagcg gcctgatgat tttggaagtc
    10981 aacacccact atccccacgg cgcaagtttc gacacgatgg tcaaagacct gctcggacgc
    11041 ggctggaaca tcatcaacgg catcgccgtc gccttcgttt tatacctgct cacctacgcc
    11101 tacatcttcg ttggcggcga cctgaccgcc aaaggcttag gcagcgcctt gggcggcaat
    11161 gtttcactca ccgtcggaca actcgtcttc ttcggcatcc tcgccttttg cgtatgggca
    11221 tccgcacgct tggtcgaccg cttcaccagc gtcctcatcg gcggcatggt attaaccttt
    11281 atttgggcaa ccggcggcct gattgccgat gccaaaatgc ccgtcctgtt cgacacccaa
    11341 gcccccgtcg gcaccaacta ctggatttac gccgccaccg ccctgcccgt ctgccttgcg
    11401 tcattcggtt tccacggcaa cgtctccagc ctgctcaaat actttaaagg cgacgcgccc
    11461 aaagtggcta aatccatctg gacgggcaca ctgattgcgc tggtaattta cgtcctctgg
    11521 caaaccgcca tccaaggcaa cctgccgcgc aacgagttcg cccccgtgat tgccgccgaa
    11581 gggcaagtct ccgtcctcat cgaaaccctg tccaaattcg cccaaaccgg caatatggat
    11641 aaaatattgt ccctgttttc ctatatggcg atcgccacct cgtttttagg cgtaacctta
    11701 ggtctgttcg actacatcgc cgacatcttc aaatggaacg acagcgtgtc cggccgcacc
    11761 aaaaccgccg cgctgacctt cctgccgcct ctaatttcct gcctgctctt ccccaccggc
    11821 ttcgttaccg ccatcggcta cgtcggcctg gcggcaaccg tctggacagg catcatccct
    11881 gccatgctgc tctaccgctc gcgccaaaaa ttcggcgcag gcaaaaccta taaagtttac
    11941 ggcggcttgt ggctgatggt ttgggtcttc cttttcggca tcatcaacat cgccgcacag
    12001 gtattgagcc aaatggaact cgtccccgta tttaaaggat aaaggcaaaa tgccgtttga
    12061 agcccgccgg cggcttcaga cggcattgcc gcaacaaacg gcaaccgtat tccggcacac
    12121 agcgcattac cctgcccctc acgcacaaat cccgccccga caaaccggga cgcaaccata
    12181 aaggaacaat gatgaagctc aaaatagata ttgcaaccaa caacttcaaa cacggcggcg
    12241 gcacggaacg ctacacattg gatttggtaa agggtctgaa cagacaaaac atcacaccgg
    12301 ccgtttatgc gacgaaattt gatcacggca ttcctgaata cgccatgatc gaaccccatc
    12361 ttgtcgatca acaccggacg ctgaaaaaac tacgctcatt cctcttttca agccggctcg
    12421 ctcaaaccag aaaaaacagt gccgccaaac tgattgcctg ccaccacgcc gattacgccg
    12481 acctcctcat ctgcggcggc acacacttgg gctacctgca ccatatggcg caaaaaccga
    12541 acctgctcga ccgcctcgcc atacgccgca accgcagcaa ctacgccacc gccaaactga
    12601 ttatggcgca ttcccatatg atgcagcgcg aactggtcgg actatacggc gttccccccg
    12661 aaagaatcca agtcgccccc ccccccgcag atacggaacg cttctttcca caacctagag
    12721 aaactgccgc tctgcgcgcc aaatacggtt ttgccgacca tgaaaccgtt ttcctatttc
    12781 catcgaccgg ccacacgcgc aaaggcctgg aactgcttgc cgactttttc gaacataccg
    12841 acctgcccgt caagcttgcc gttgtcggct ccccgctccc ccgccctatg aaaaacgtcg
    12901 tcggactggg cttctgcacc gatatgcccg aactctaccg cgccgccgac tttaccatta
    12961 tggcctccct gtatgaaccc ttcgggctgg tcggtgtcga atccgtccta tgcggcacac
    13021 gcgtcgtcct ctccgaaaac atggcatgta cagaggtcat gaacgaagaa gccggattct
    13081 ttttctcacg ccaaaacccg gaaaccctag cgcaagccgt tgcccaagcc gtcagcctta
    13141 aaaaacaggg cggacaccgc ctgtccgacc cgatgcgtgc actgaactac aacccgtccc
    13201 tgtcgcacca catcgaccga ctgaccgaca tactggcatc cgtataacaa aatgccgtct
    13261 gaacgagttt tcagacggca ttgctgc
//
LOCUS       DAWHXS010000058        12855 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWHXS010000058 DAWHXS010000000
VERSION     DAWHXS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.058"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(53..361)
                     /locus_tag="WIE22_001277"
     CDS             complement(53..361)
                     /locus_tag="WIE22_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127356.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WIE22_001278"
     CDS             complement(345..647)
                     /locus_tag="WIE22_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ4127357.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WIE22_001279"
     CDS             complement(721..3084)
                     /locus_tag="WIE22_001279"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ4127358.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WIE22_001280"
     CDS             complement(3120..3638)
                     /locus_tag="WIE22_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127359.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WIE22_001281"
     CDS             complement(3645..4709)
                     /locus_tag="WIE22_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ4127360.1"
                     /translation="MIKSFELENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIE22_001282"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIE22_001282"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ4127361.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIE22_001283"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIE22_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ4127362.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIE22_001284"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIE22_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ4127363.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIE22_001285"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIE22_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ4127364.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WIE22_001286"
     CDS             complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WIE22_001286"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ4127365.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLGAEYVTENNDRSRPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="WIE22_001287"
     tRNA            complement(9446..9522)
                     /locus_tag="WIE22_001287"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="WIE22_001288"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="WIE22_001288"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ4127366.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            10898..11908
                     /locus_tag="WIE22_001289"
     CDS             10898..11908
                     /locus_tag="WIE22_001289"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ4127367.1"
                     /translation="MLPFFPEPSLSYTQQNRTAVLLLNLGTPDAPTAQAVRPYLKSFL
                     TDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYEKIWFKEGSPLEVYTARQAAALAER
                     MPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLVIPMYPQYAASSSGAAVDKVCEQLL
                     LQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAEHGRGKKLMLSFHGVPQKHYDLGDP
                     YPDECRHTAKLLAEALELTEDQYVVSFQSQFGRAKWVTPSTQDLFGKLPKQGVTELDV
                     FCPGFLADCLETMEEIALMGREQFYEAGGKSYRYIPCLNDNPDWIDALVALAEENLGG
                     WR"
     gene            12100..12501
                     /locus_tag="WIE22_001290"
     CDS             12100..12501
                     /locus_tag="WIE22_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ4127368.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12564..12854)
                     /locus_tag="WIE22_001291"
     CDS             complement(12564..12854)
                     /locus_tag="WIE22_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127369.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3249 a         3407 c         2865 g         3334 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgacgttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggca ggatgtccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgcgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggcccaa
     1861 acgcaatccg acctgcttcg cttcgggcaa ttcgccttgc gcttccacca tttcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgc tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaagcga ccgcaatcgg caggcgcgtc
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tgcccggcaa cactgcgccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttcg gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgaa taggagaatt gcatgttatg ttctcaatca attaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gctcaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagcca cattgtaaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cagaatcgat attcacattt ttcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttct cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgcttttaag tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatca ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaatg tcgagggctt cggcggcaag tcgggcttgc agcttggctt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatatgccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttcg atagaggcgc gtttcagacc attcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtcg cggtatgcgt attggccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc ggtagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgggcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc atttaccata gaggtaccgc
     6661 gcagttggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcagataa ggcattaggg ggttttaaaa
     6841 catcggtttc aaacacttga acccacaata ccacggtttt ttgccgcaat tgaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct ttcacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttgatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttga tttccttgat
     7201 ttgcacctgc ttttggtttt tcttggcttc gtcgcgtttc ttggcctgct ggtatttgta
     7261 tttaccgtaa tccatcagtt tgcacacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacaa caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caaatcttct
     7561 gctttgcggc gtaccgccac tttgttttcc tgcttctcct tatcgccgac aacgatttga
     7621 taagggaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcccg acaataatct
     7741 gcctgatttt cggtaatatt cataatcacc atttgaaccg gagccaacca taacgggaaa
     7801 gagcctgcat ggttctcaat cagaatgccg ataaatcgct ccaaagaacc taaaatggcg
     7861 cgatgcaaca taacaggtcg cgagcggtcg ttgttttcgg ttacatattc tgcacccaag
     7921 cgttccggca agacgaaatc cagttgtaat gtaccgcatt gccaagaacg acccaaggca
     7981 tctctgacat gatattcgat tttaggcccg taaaacgcac cctcgcccgg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcacgttt ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg cacgggcctc gctgacgatt
     8221 tggtcttcgg tacagaaaat atgcgcatca tcctgcacaa atccacggac ccgcatcaga
     8281 ccatgcaatg caccgctcgg ctcgttgcgg tggcaggaac cgaattccgc caaacgcatc
     8341 ggcaaatctc gatacgaacg caaaccgttg ttaaaaattt gaacatgacc cggacagttc
     8401 atcggtttaa ccgcatattc gcgtttttcc gaactggtta cgaacatatt atctttgtag
     8461 ttgtcccaat ggccggattt ttcccaaaag gttttatcca tgatttgagg cgttttgacc
     8521 tctttataac cggcggcgtt cagttcttta cgcatatgct gttcaatcac ttgccacaaa
     8581 gcccagcctt taggatgcca aaacaccatg cccggcgctt cgtcttgcag atggaacaga
     8641 tccaattgct tgccaagctt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt atatgcgttg cagcatttca
     8761 ttattgctgt cgccgcgcca gtatgcgccc gccagcttgg tcagtttgaa gtttttcagg
     8821 aaacgggtat tcggaacgtg cggaccgcgg cacatatcga catattcctg gtgatgatac
     8881 atccccatcg cttccacttc gggcatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattcttt catacgcgct tcaatggcgg caacatcttc cggtgtaaac
     9061 ggtttttccg tggcgatgtc gtaataaaag ccctcttcaa tgacggggcc gataaccatt
     9121 tttgcattag gatagagttg cttgacggca tgcccgacaa gatgggcgca ggaatggcgg
     9181 atgatttcga tgccttcctg atctttcgga gtaatgattt gaacagcaga attttcaaca
     9241 atcgggtcgc acgcatcgac caatttgccg tttaccctgc ctgccaccgc cgccctcgcc
     9301 aaaccgacac cgatagacgc agcaatttga gccacggtaa cgggggattc gtattggcgg
     9361 actgagccgt ccggcaaggt aatgttcaac atcaaaaagc tccaaaacat taaaaaataa
     9421 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcatt tctttatgca acattgtggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
     9661 acatatttcg ccccctgtcg cccaactgac attcaatctt atgcttaaat ttaccttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaattgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ctcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 cttggaggtc gtcgaacagt ttggagggct tcacggcttt atcggctggg acaaaccgat
     9961 tctgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgttcaaaa gcccgatcaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttccatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagctgg acgaatgcac
    10141 gccgggcgag gcgacgcgcg aacaggcgcg aaaatcgttg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcactgt tcggcatcgt
    10261 gcaaggcgcg atgtatgaag atttgcgcga agagtcgctg aaaggcttgg aagagctcga
    10321 tttccccggc ttggccatcg gcggcttgtc cgttggcgaa cctaagcccg aaatgtaccg
    10381 catgttgcgc gccgtcggcc ctatcctgcc ggaacacaaa cctcattatt taatgggcgt
    10441 cggtacgcct gaagacttag tgtacggcgt ggcacacggc gtggatatgt tcgactgcgt
    10501 catgcctact cgcaatgcgc gtaacggctg gctgtttacc cgttttggcg atttgaagat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg
    10681 cgcacagctc aacaccatcc acaacctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgatgccata gaacaaggca aatttgccga ctggcaggcg cgattccacg aaaaccgtgc
    10801 tcgcggcaca gactgactcc cggcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattct ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgtt
    11101 gcgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgcccct
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccgaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acggacgtgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattctg cccgggcttt ttggcagact gtttggaaac
    11761 catggaagaa atcgccctga tggggcggga acagttttac gaagcaggcg gcaaaagcta
    11821 ccgctacatc ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgccgga
    12001 caacaacatc aagtgcaacc ttactaccga ttcggcaaaa aaatctttca cccgctacgc
    12061 aaaatcaaca acaaggtttc aaacattaag gaaacaccaa tgaacacaac ccgactgccg
    12121 accgccctcg tcttgggctg cctctgcgcc gccgcttctg ccgccgacaa cagcatcatg
    12181 acaaaagggc aaaaagtgta cgaatccaac tgcgtcgcct gccacggcaa aaagggcgaa
    12241 ggccgcggaa ccatgtttcc gccgctctac cgctccgact tcatcatgaa aaaaccgcag
    12301 gtgctgctgc acagcatggt caaaggcatc aacggtacaa tcaaagtcaa cggcaaaacc
    12361 tacaacggat tcatgcccgc aaccgccatc agcgatgcgg acattgccgc cgtcgccact
    12421 tatatcatga acgcctttga caacggcggc ggaagcgtta ccgaaaaaga cgtaaaacag
    12481 gcaaaaggca aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
    12541 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
    12601 ccgtccccgt caacctcctt cgcggtcagc ataaagccgc cgatgtcaat acggccgcac
    12661 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
    12721 tcgcccgctt ccagcctcaa accgtaagca agtgccaaat cacccgaacg tgctgccggc
    12781 aaaacgacaa actcaccgaa gaaatcaaaa tactcacgga cgctaatacc cgtctcggta
    12841 aagtatttcg ccatt
//
LOCUS       DAWHXS010000059        12815 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWHXS010000059 DAWHXS010000000
VERSION     DAWHXS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12815
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.059"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..325
                     /locus_tag="WIE22_001292"
     CDS             <1..325
                     /locus_tag="WIE22_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T cell stimulation protein TspA"
                     /protein_id="HEZ4127370.1"
                     /translation="TEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTGFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIE22_001293"
     CDS             complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIE22_001293"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ4127371.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIE22_001294"
     CDS             complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIE22_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ4127372.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2119..2433)
                     /locus_tag="WIE22_001295"
     CDS             complement(2119..2433)
                     /locus_tag="WIE22_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ4127373.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            2743..3741
                     /gene="ilvE"
                     /locus_tag="WIE22_001296"
     CDS             2743..3741
                     /gene="ilvE"
                     /locus_tag="WIE22_001296"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ4127374.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3926..4711)
                     /gene="fabI"
                     /locus_tag="WIE22_001297"
     CDS             complement(3926..4711)
                     /gene="fabI"
                     /locus_tag="WIE22_001297"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ4127375.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4866..5687)
                     /gene="dapD"
                     /locus_tag="WIE22_001298"
     CDS             complement(4866..5687)
                     /gene="dapD"
                     /locus_tag="WIE22_001298"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ4127376.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5854..7497)
                     /gene="pgi"
                     /locus_tag="WIE22_001299"
     CDS             complement(5854..7497)
                     /gene="pgi"
                     /locus_tag="WIE22_001299"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ4127377.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            8007..9239
                     /gene="pilG"
                     /locus_tag="WIE22_001300"
     CDS             8007..9239
                     /gene="pilG"
                     /locus_tag="WIE22_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ4127378.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9313..10173
                     /locus_tag="WIE22_001301"
     CDS             9313..10173
                     /locus_tag="WIE22_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ4127379.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10175..10807
                     /locus_tag="WIE22_001302"
     CDS             10175..10807
                     /locus_tag="WIE22_001302"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ4127380.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            10800..11009
                     /locus_tag="WIE22_001303"
     CDS             10800..11009
                     /locus_tag="WIE22_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ4127381.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(11094..12770)
                     /gene="pilB"
                     /locus_tag="WIE22_001304"
     CDS             complement(11094..12770)
                     /gene="pilB"
                     /locus_tag="WIE22_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ4127382.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPILFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2938 a         3558 c         3358 g         2961 t
ORIGIN      
        1 aacggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccggtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggtcgacgg caacaaaatg ccgtctgaaa cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcgcc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc atcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcattttttg tgttcctttc ggtggtggtt aaacttcgat tttattcggg
      961 gtaaacgtct gccatttgtt gcaggcgggg cagtgccaga aaaagacttg tgatttgaag
     1021 tggcagtttc ggcaccggta catcacgctg cgctgtagct gccgtccgat aaccgaacgc
     1081 atcatatcgg catcggcttt ccaagccgga tccaaatcgc tgagtttcaa accaagcagg
     1141 cggtacacgc cgttgaggtc gggcttgcgg cggacaagct cgacggcggt ttgcgcggct
     1201 tctttctcgc acttaagcag cagggatttc tcgtacacga cattgatcag gtcaagttcg
     1261 ggaaacgtct gcatatatcc tgtcagacgg ttcaagcctt cttcaggttt tccctgcgcg
     1321 gcataggctt cgtaaagctt ctcgccgacc atgctcaagt atgcatggtt ttgctgctcg
     1381 atggcggcat aggcttcgac ggcggcaggg aaattgcctt gtcggtgttc gatgtcgccc
     1441 aaaatcatgt tggcacgggt gcattttttg ttggcttcga gtgccttgcc gacattgaaa
     1501 cgcgcgacat cgaaattgga cttgaacagc gcggcttggg caagttcgca ataaaactgg
     1561 gcgatttcaa actgataggt ctgatcgtca tggctgagca gccgggcggt ttcaaccgct
     1621 ttttcccaat ccctgtcctg ttggtagata ttgagcaggt gctgtctggc ttcacgcgcc
     1681 attttaccgt cttgcagccc caaaaaaatc tgttcggcac gatcgaccaa ccccgcactt
     1741 tggtagtttt gcgccaattc aaacaggacg cgcgcgcgct tttcgccgac cgtatcggga
     1801 gaatcgagca ttgtccggtg tatgttgatg gctttgtcgt tttcgccacg ctgacggtaa
     1861 agcttgccta gggtgaggtt taggtcgtat gactgaggct gctgatcaat gacctctgcc
     1921 aattccctgg cggcacgtcc gctgttgcgg tcaaccaagg catccagact tttataaaat
     1981 cccgacggta tgctttttgc ctgctttaat acagtcttca tatccacacg ggcggcaaac
     2041 cagcccatcg cgaagaaaac gggcaaaagg ataatcggca gcaggataat ccacaattca
     2101 ttgtccatat cggctttctc aaggctgttt ggcagattcg ggcgcatttt gcgccggtgg
     2161 tgcggtcagc tctttccccg tcaaacgcgc atttttcttt acttcggcac gcaacctgcc
     2221 gttctcgcca cgtaacgaca acaaccgtcc gaacaaggca aacattccga acacgatgcc
     2281 gacgacaaac gcgccgaaca atacgacaat cagcggcaaa tcgactttct gtccgggcag
     2341 gtaggagaag gtaacggcat ccgtattaat gacggcaagc agcaggaaga gcagcaggat
     2401 aatgattttg atgacggtat agataagttt catgtttgct ccgtaaataa aaacgccgcc
     2461 cggacagctt cgcactctga accaagattg cgctagttta aacgatttgc acggtttcgg
     2521 ataggatgcg gcagcgtgtt cggacgacat acggagtatg cgattgccga caattttacc
     2581 aaatacaccg ttcctttcca tttgaaaaat aacggattgg acaccgcatc gacagaaaaa
     2641 cccgccgcgc acttgtcaaa accctgtttg caggcgtatc tttacaatct tcaaattcaa
     2701 accgttcatt gaaacatatc agaataagaa aggctttaca tcatgagcag acccgtaccc
     2761 gccgtattcg gcagcgtttt tcacagtcaa atgcccgtcc tcgcctaccg cgaaggcaaa
     2821 tggcagccga ccgaatggca atcttcccaa gacctctccc tcgcaccggg tgcgcacgcc
     2881 ctgcactaca gcagcgaatg tttcgaggga ctgaaagcct tccgtcaggc agacggcaaa
     2941 atcgtactgt tccgtccgac tgccaacatc gcgcgtatgc ggcaaagtgc ggacattttg
     3001 cacctgccgc gccccgaaac ccaagcctat ctcgacgcgc taatcgaatt ggtcaaacgc
     3061 gccgccgatg aaattcccga tgcgcctgcc gccctgtacc tgcgtccgac cttaatcggt
     3121 accgatcccg tcatcggcaa ggccggttct ccttccgaaa ccgccctgct gtatattttg
     3181 gcttcccccg tcggcgacta tttcaaagtc ggctcgcccg tcaaaatttt agtggaaacc
     3241 gaacacatcc gctgcgcccc gcatatgggc cgcgtcaaat gcggcggcaa ctacgcttcc
     3301 gccatgcact gggtgctgaa ggcgaaagcc gaatacggcg caaaccaagt cctcttctgc
     3361 ccgaacggcg acgtgcagga aaccggcgcg tccaacttta tcctgattaa cggcgatgaa
     3421 atcattacca aaccgctgac cgacgaattt ttgcacggcg taacccgcga ttccgtactg
     3481 acggttgcca aagatttggg ctataccgtc agcgaacgca atttcacggt tgacgaactc
     3541 aaagctgcgg tggaaaacgg tgcggaggcg attctgaccg gtacggcagc cgtcatctcg
     3601 cccgttactt ccttcgtcat cggcggcaaa gaaatcgaag tgaaaagcca agaacgcggc
     3661 tatgccatcc gtaaggcgat taccgacatc cagtatggtt tggcggaaga caaatacggc
     3721 tggctggttg aagtgtgctg atgctttaaa taaaaaaatg ccgtctgaaa cccgtttggc
     3781 gtttcagacg gcattttcgc gttcggacca tttccgctgc acctgcagca agtcgggcat
     3841 ttccctagaa atacgcttca gacggcaaac cgccgcccga aaccgatacg cggtacggac
     3901 ggcgcacgga tttgaaaacg gcggattatc cctcggtgct caaggcatta atgctgtaac
     3961 cgccgtcaac gtaagtgatt tcgccggtaa tgccggacga caggtcggac agcaggaagg
     4021 cggcggtatt gccgacttct tcaatggtaa cgttgcggcg cagcggattg tgggcggcga
     4081 cgtgtcccaa aagtttgccg aaatcggcga taccggaggc ggcaagcgtt ttaatcgggc
     4141 cggcggaaat gccgttgcag cggatgcctt ctttgcccag acaggcggcg gtaaaacgga
     4201 tgcccgcctc aaggctggct tttgccatac ccatcacgtt gtaatttgga atcgcgcgca
     4261 ccgcgcccaa gtagctcagg gcaacgatgg cggagtttct gccgcgcatc atcggacggg
     4321 cggcttttgc caatgcgggc aggctgtatg cggaaatttc gtgtgcggtg ttgaacgctt
     4381 cgcggctgat gctgtcgagg aagtcgccgc tcaaggcttc tttcggcgcg aagccgatgg
     4441 aatgcaccaa accgtccaag ccgtcccaat gtttgcccaa gtcggcgaac acttggttga
     4501 tttcgtcgtc gctggcgaca tcgcagcgga atacgagttc ggaacccaat tccgccgcca
     4561 ttttgcggac gcgctcttcc agtttgtcca caacgtaggt aaacgccagt tccgcgcctt
     4621 gttcgcggca ggctttggcg atgccataag cgatggaacg ctcggaaatc atgccggtaa
     4681 tcagaatttt tttgccttgc agaaaaccca ttttgtatcc ttcaaatagt gttcggttca
     4741 ttctaaaccg ttgattatag caaattgtcc ctgtttctgt gttttcacgt tgcagcatgc
     4801 aaacggcaat gccgtccgaa gcggatttca gacggcatcg gacgtttcaa atacggttta
     4861 aggcatcaga tgccgcgcaa caattcgttc acgctggttt tcgcacgggt ttgcgcgtcc
     4921 acgcgtttga cgatgacggc gcagtaaagg ctgtggctgc cgtctttgga aggcatactg
     4981 ccggacacga cgaccgaacc ggccggtacg cggccttggt agatttcgcc ggtggtgcgg
     5041 tcgaagattt tggtggattg accgatgaac acgcccatag aaatcacgct gccttcttcg
     5101 acaatcacgc cctcaacgat ttcggaacgc gcaccgatga agcaattgtc ttcaataatg
     5161 gttgggctgg cttgcagggg ttcgagtacg ccgccgatgc cgacaccgcc gctcaagtgg
     5221 acgtttttgc cgatttgcgc gcaagagccg acggtcgccc aagtatcgac catcgcacct
     5281 tcgtcaacgt atgcgccgat gttgacgtaa gacggcatca gcacgacatt tttcgccaca
     5341 aagctgccgc gtcgggcaac cgcacccgga acggcgcgga agcctgcgtt tttgaactcg
     5401 tcttcagacc agtcggcaaa cttggtcggc actttgtcga agtatttgtt cacaccgtcg
     5461 ttgaggattt cgttgtcttg gatgcggaag gacagcaaca cggctttttt cgcccattcg
     5521 ttaactttcc attcgcccac gcccaagcgt tcggcgacgc gcagtttgcc ggaatcgagt
     5581 tggcggatgg tttccaacac ggcttcttta acttcgggag taacggtggt cggattaatg
     5641 tccgcgcggt tttcaaaggc ggtttcgata atgttttgca aagacataat gtttccttat
     5701 gtgagatgtt tcgtgggacg cttgccggta ttttcaagca gtatgttcat ttgaaatgaa
     5761 tgcgctattg tgccacgcaa acgcatattc gtcagcaata cgcgggcggt tgccgaacat
     5821 tggcgtttcg gaatcgggcg ggcggcagta cctttatgcc gcaccgccgc ctttcagacg
     5881 gcattcgcgg taaaacgcca tcagcccttc ggtcgaggca tcgtgtacgg acgtgccgcc
     5941 ttccagttcg ccgatgatgg tttttgccaa ctgtttgccg tattccaccc cccactgatc
     6001 gaaggggttg acgttccata tcgcgccttg gacgaaggtt ttgtgttcgt aagccgccat
     6061 cagcataccc aaattgtagg gcgtgaggcg gtcgagcagg atgctgttgc tggggcggtt
     6121 gccagggaac tctttgtgcg gcgcgaggcg ttcgcgttcc gcttcgggca aatctgccag
     6181 ttcggcgcgt gcttcgtcca aggttttgcc cttcatcagg gcttccgctt gggcaaaggc
     6241 gttggcaacg gtaaaacggc tgcgtccgtc ctccacgccc tgcgccgtca tcgggacgat
     6301 aaagtcgcag ggaatcaggc gcgtgccttg atggagcagt tggaaatagg cgtgctggca
     6361 gttgacccct tcaccgccga acacgatgcc gcccgttttg cacacggcgg gactgccgtc
     6421 tgaagcgcgg cttttgccca aactctccat atcgagctgg ttcagccaca ccggcagcag
     6481 gcgcaggttg tggctgtacg gaacggcggt ctgcccgtcc gcgtgttgga aattgttata
     6541 ccacacggca atcagtgcca tcagaacggg aatgttgcgg cgcggcggcg tgtggaaaaa
     6601 atggctgtcc atcgcgtgcg cccccgccaa caactcgcgg aaacgcgccc cgccgaccgc
     6661 aaccatcacg ggcaaaccga cgggcgacca gacggaatag cgtccgccca cccagtcgta
     6721 catcgcaaac acgcgttccg ccgcgatacc aaaagccgca gctgccgcag tgtcggcaga
     6781 caccgcgcaa aaatggcacg ccgtttcgga ttccgagaac cccgcaccgc gataccacgc
     6841 cttgactgcc tgtgcattga gcagggtttc cggtgttttg aaggacttgc tggcaacgca
     6901 aaacactgtc gtttcggggt tcagacggca taaaaccgca tccaggcagg caggatcggc
     6961 gttggcggca aaatggacgg tgatatgccg tctgaacggc tcaagtgcct gcacgcacat
     7021 tgccggcccg aggtcggatc cgcctatgcc gatgtggaca aaatccgtaa tccgttttcc
     7081 ggttgtcccc tgatacgaac cgtcgtccaa actgtgtgca aactccaacg cacgatttaa
     7141 ctcgcggcgg atttcgggca acacgtccct gccgtccgca taaacggcat ccgcaccgtc
     7201 gggtaggcgc aaagccgtat gcagcgcggc acgcccctcg ctgccgttga ctttcgcacc
     7261 cgtccgcaaa gcacgcattt tcccttccaa atccgccgtc tccgcaagac ggcagagcag
     7321 ttgcagcgta tcttcgccca aacggttttt gctgtaatcg aacaacatcc cgtccaaacg
     7381 ctcatgcata cgctcaaaac ggtcgggttc gcaggcaaag cggtcgcgca aaaggacatg
     7441 acgcgtatcc tgataatggc gttcgagcgc ataccatgca cgggtaaaag cattcatctg
     7501 ttttccttga tttttcagaa ccggattaaa atgtagcaga atgtagttta acaaacggca
     7561 gcggctttgg cgaatctccg gaacaccgcc cgccgcgtcc aatagcacct tatttccata
     7621 tggcgaaaat atagtgaatt aacaaaaatc aggacaaggc ggcgagccgc aaacagtaca
     7681 aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctcttgag
     7741 ctaaggcgag ccaacgctgt accggttttt gttaattcac tataccctcc ggccggcatt
     7801 tgacccgcaa caatatcctg caagatttat tgtgtacgca taaatgccgg acagccgcct
     7861 aaataaggta taatcgcatc cgattctgtc ccgtttgccg cccgcaggcg ggatgtcggc
     7921 ggttttcatc ttaccgggaa cacgccggta ctgtttttta acgatgtttc ttacatttta
     7981 ttccaattac tttacggggc tagaatatgg ctaaaaacgg aggattttct ttgttcgcaa
     8041 agaaagaaaa acgctttatc tttgaaggca ggcattccgc ctccgacaaa ctggtcaacg
     8101 gcgaagtatc cgcgtttacc gaagaagagg cgcgcaaaaa actggcaaaa cgcggcatcc
     8161 gcccgttgca gattacccgt gtgaaaacaa gctccaagcg caaaatcaca caagaagaca
     8221 tcaccgtttt cacccgccag ctgtccacga tgattaaagc gggcctgccg ctgatgcagg
     8281 catttgaaat cgtggcgcgc ggacacggca acccgtctat gacggaaatg ctgatggaaa
     8341 tccgaggcga agtggaacag ggcagctcgt tgagccgcgc attctcaaac cacccaaaat
     8401 atttcgaccg cttctactgc aatctggttg cggcgggcga aacgggcggc gtattggaaa
     8461 gcctgctgga caaattggca atttacaaag aaaaaaccca ggccatccgc aaaaaggtaa
     8521 aaaccgcact gacctatccg gtatccgtca tcgccgtcgc catcggtttg gtattcgtga
     8581 tgatgatttt cgtactgccc gcctttaaag aagtttacgc caatatgggc gcggagcttc
     8641 ccgcactgac ccaaacagtg atggatatgt ccgacttttt cgtctcatac ggctggatgg
     8701 tgctgatcgc actgggcttt gccatatacg gcttccttaa attgaaggcg cgttcgatta
     8761 aaatccaacg gcgtatggat gccatactgc tgcgtatgcc gattttcgga gacattgtcc
     8821 gcaaagggac gattgcccgc tggggcagga cgacggcgac gctgattgcg gcaggcgtgc
     8881 ctttggtcga tgtattggac tccactgccg gcgcggcggg caatttaatc tatgaagaag
     8941 ccacccggga aatccgtacg cgcgtcatcc aaggtttgtc tatgacttcg gggatgcgtg
     9001 cgacggaact gttccccaat atgatgttgc agatgtcttc catcggcgag gaatcgggtt
     9061 ctttggacga tatgctcaac aaagccgccg aattttacga agacgaggtg gacaatgcgg
     9121 tcggcaggct gtccgctatg atggagccga tcattatcgt gattttaggc ttggtcatcg
     9181 gcacgcttct ggtcgccatg tatctgccgc tgtttaattt gggcaacgtg gtcgcctgat
     9241 ttgccgcaca gatccggcgc ggattcgctc tgcgccggtt tgtttttgtt ttgaatacat
     9301 tgagaacaaa atatgtctga tttgtctgta ttgtcgccgt ttgccgtgcc tttggcagcg
     9361 gtgttcgggc tgctggtcgg cagtttctta aacgtcgtca tttaccgcgt gccggtcatg
     9421 atggaacgcg gctggacggt atttgccaaa gaatatttaa acctgccgct gaccgaagag
     9481 gaaagccgta ccttcaacct gatgaaaccg gattcctgct gtcccaaatg ccgcgtgccg
     9541 atacgcgcgt ggcagaacat cccgattgtc agttacctgc tcctgcgcgg caaatgcgcc
     9601 tcctgtcaaa ccaaaatcag catacgttat cccttaatcg agctgctgac cggcgtattg
     9661 ttcgggctgg tcgcctggca atacggctgg tcttggatta cgctgggcgg attggtactg
     9721 accgcgtttc tgatttccct gacctttatc gatgcggaca cccaatacct gcccgactcg
     9781 atgacactgc ccttgatttg gctggggctg atatttaatt tggacggcgg cttcgtgcct
     9841 ttgcagtctg ccgttttagg tgcggttgcc ggctatggtt cgatatggct tttatgcgcg
     9901 gtatataaac tgattaaagg cgaaatcggt atgggcaacg gagatttcaa actgattgcc
     9961 gcattgggcg cgtggctcgg catatccgca ttgcccgtgc tgatttttgt ttcctcgctg
    10021 atcggtttgg tcgcggcaat cgttatgcgc gtcgccaagg ggcagcattt tgccttcggc
    10081 cccgcactga cagtttcggg ctggataatt tttacggcaa acgattccgt atggcgggcg
    10141 gtcaactggt ggctgaccca tccggtgctg taagatgacg gtatgggtcg gactgaccgg
    10201 cggaatcggc agcggcaaat cggcagccgc gcaatgtttt gccgatttgg gcgtgccgcg
    10261 catcgatgcg gacgcggcgg cgcactcgct gacggcttca gacggcatcg ccctgccgga
    10321 aatcaggcgg ctgttcggcg acaccgtttt cgacacacag ggtttgttgc gacgcgacat
    10381 attgcgtaaa gaagtctttg cctccccatc gcgaaaagcc ttgctcgaat ccgtgatgtt
    10441 gccgctgatt ttctcagaaa tcaaaaaaca gcaagaaacc tttaccgatg cggcttacgg
    10501 cattgtcgaa attccgctgc tgacggaaaa gcgtcaattt atcagcctga tacggcgtgt
    10561 cctgaccata agcgcacctt tggaaaaacg tatcggcagg gtgatggccc gcagcgggct
    10621 gacgcgcggc gaggtggcgg acatcatcag ccatcaggca tccgaatccg aacgcctgct
    10681 gcttgcagac gatgtgctgc tcaatgacgg cagcctcaaa agcctgcgtg agaaaacaat
    10741 gcgcctgcac gcgttttatt cagggatttt cgcctcaaaa ccaacacaag gaaaacacaa
    10801 tgactgaatc gcggcaaaca cgccttcaag tcaaatgtcc gacctgtcaa acagcagtag
    10861 tatgggaacc cgaaaacgca ttccgcccct tctgttcgca acgctgcaaa ctgatcgact
    10921 tgggcggatg ggcagacggg aaatatacgg tttccggcca aacggaaagt ttgccggaaa
    10981 tatccgaacc cgacatggca taccgctgac cgccccgcct tcccggcaaa caccctgaaa
    11041 gtcaaatgcc gtctgaaaca aacacgcttc agacggcatt ttcattctca aacctaatcg
    11101 ttggtatttg ccgttacctc ttccaatgaa gtaatgccct gcataacttt caaaataccg
    11161 gcccggcgca aatccaccat accctcctta taggcaacgt ccaaaatatc cacttccgta
    11221 ccgttgttca taatcacacg ctgcatttct tcgctgatgg gcataacctc atacacgccc
    11281 gcacgcccct tataaccctg cccccggcaa cggtcgcaac cgacggcgcg gtaaagtttc
    11341 caatcttttg caaggtcctc atcggtgaag ccgacttcct tcaaagcaga ggcagacggg
    11401 cgttccactt cctgtttgca gctcgaacac agcctgcgta aaagacgctg cgccataatc
    11461 aggctgaccg aactggcaat attaaacggc gcgacaccca tattcagcat acgcgacaac
    11521 gtcgccggcg cattattggt gtgcagggtg gaaaacacca tatgccctgt ttgtgccgcc
    11581 ttaatcgcaa tatcggcagt ttccaaatca cgaatctcac cgaccataat gatgtccggg
    11641 tcctgacgca ggaaagactt caaagcagcg gcaaaagtca ggccctgctt atcattgacg
    11701 ttaacctgat tgatgcccgg caggttaatc tcggcagggt cttccgccgt tgcaatattt
    11761 accgactccg tattcaaaat attcaaacag gtatagagcg acaccgtctt acccgaaccc
    11821 gtcggaccgg ttaccagcac catcccgtag ggacggtgaa tcgcttccaa caacaatttt
    11881 ttctgaaacg gctcaaaacc gagctggtcg atgttcaaag acgcggcatc ggaattcaaa
    11941 atccgcatca cgaccttttc gccaaacagc gtcggcaatg tgctgacacg gaaatcgaca
    12001 ggcttgccgc ccttttgaaa ggtcagctgc atcctgccgt cctgcggtat ccgtttttcg
    12061 gaaatgtcca aacgcgacat taccttaatc cgggaagcaa gctgccccct taccgcaatg
    12121 ggcggctgaa ccacctcgcg gagctgcccg tccacacgga aacggatacg ggcattgtgt
    12181 tcgtaaaact cgaaatggat gtccgatgcc ccgctgcgca aggcatccga caaagtctta
    12241 tggataaacc tcggaacagg gccgtcttct gcctcctcgt tgtcgatata cagggtgtgg
    12301 ctttcctctt cctcctgccc ctccccaagc tcctgaagca gcgatgtcga acgcgaaccc
    12361 acccaatcga gcaaacccgc caactggtca tcctcgacaa tgaccaactc aacctcaatc
    12421 cctgcggcag aaacggtttt ctgaatttgc ggcatctgtg tcggatcgga aaccgcaaaa
    12481 aatactttgt cgccccgacg gaaaaccggc acacagtgga actccaccat ctgctcctcc
    12541 gtcaacaccc ccatcagcac cctgtggcgc ggataatgac gcaaatcaag aatcgaataa
    12601 ctgaacaccc ttgcaatcaa tgccgcaagc gacttgggcg aaatgacgcc gtctgaaaac
    12661 agtatcggca acacttcctt acccgcctgc gactccttgt agtaatgctc ggcctgctca
    12721 acagtaacca cctggttttg aaccagaatc ctcagcaaac ctacgctcat acgaccttat
    12781 ccccaaattt attcattgtt atacctgtac agctt
//
LOCUS       DAWHXS010000060        12389 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWHXS010000060 DAWHXS010000000
VERSION     DAWHXS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.060"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1599)
                     /gene="dnaX"
                     /locus_tag="WIE22_001305"
     CDS             complement(<1..1599)
                     /gene="dnaX"
                     /locus_tag="WIE22_001305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ4127383.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSESKTAEPVTNQENNDVPPWEDAPDEAQTAAGTT
                     AQASAKSIQTASEAETSPDNQVSKNKAADNETETSLSEVPSENPIQATPNDEAVETEA
                     FAHEAPAEPFYGYGFPDNDCPPEDG"
     misc_feature    complement(371..410)
                     /gene="dnaX"
                     /locus_tag="WIE22_001305"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(1694..3670)
                     /gene="mutL"
                     /locus_tag="WIE22_001306"
     CDS             complement(1694..3670)
                     /gene="mutL"
                     /locus_tag="WIE22_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ4127384.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPEQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVVSTPMPSENDSENLFDRASNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAINTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            3813..3998
                     /locus_tag="WIE22_001307"
     CDS             3813..3998
                     /locus_tag="WIE22_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ4127385.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            4009..4677
                     /locus_tag="WIE22_001308"
     CDS             4009..4677
                     /locus_tag="WIE22_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ4127386.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            4677..5393
                     /locus_tag="WIE22_001309"
     CDS             4677..5393
                     /locus_tag="WIE22_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ4127387.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            5441..5926
                     /gene="purE"
                     /locus_tag="WIE22_001310"
     CDS             5441..5926
                     /gene="purE"
                     /locus_tag="WIE22_001310"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ4127388.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLNMELEQ
                     I"
     gene            complement(6109..7563)
                     /locus_tag="WIE22_001311"
     CDS             complement(6109..7563)
                     /locus_tag="WIE22_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ4127389.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(7560..8261)
                     /locus_tag="WIE22_001312"
     CDS             complement(7560..8261)
                     /locus_tag="WIE22_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ4127390.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPTDLEENA"
     gene            complement(8258..9037)
                     /locus_tag="WIE22_001313"
     CDS             complement(8258..9037)
                     /locus_tag="WIE22_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ4127391.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            9321..10727
                     /locus_tag="WIE22_001314"
     CDS             9321..10727
                     /locus_tag="WIE22_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4127392.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFILGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTLS"
     gene            10839..12365
                     /locus_tag="WIE22_001315"
     CDS             10839..12365
                     /locus_tag="WIE22_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ4127393.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2600 a         3384 c         3423 g         2982 t
ORIGIN      
        1 gccgtcttcc ggggggcagt cattatccgg aaagccgtaa ccgtagaaag gttctgcagg
       61 ggcttcgtgc gcaaatgctt ccgtttcaac ggcttcatca ttcggtgttg cctgaatggg
      121 gttttcagac ggcacttcgg acaaggaagt ctcggtttcg ttgtcggctg ccttgttctt
      181 ggaaacttga ttgtcaggcg atgtttcggc ttcggatgcc gtctgaatgc tttttgccga
      241 tgcttgcgcg gtcgtgccgg ctgcggtttg ggcttcgtcc ggcgcgtctt cccaaggcgg
      301 aacatcgttg ttttcttgat tggtaacggg ttcggcagtt ttgctttcag acggcattgc
      361 cgctgcggat gccgtctgaa cgggtgtttg ggcggtttcc gcttcagggc gcggttgggg
      421 cttttttgcg gcggtttcct tttcggcggt ttgtgccgat ggggattgaa gttcggtatt
      481 ttcaatcacg gcgtttgcat cacacgaagc tgccgccaag ggcgcaaacg ccagcatacg
      541 cagcagggtc atcataaagc cggcgtattc gtcgggggcg aggcccaagt cgcgtttgcc
      601 gtggacggcg atttggtagt aaagctggat ttgttcgcca cctatggttt gggcgaggcg
      661 gtgcaaaata tcggaatcgg ggtcgtcgtg cgccaaggca ttcggcactg cctgtatcag
      721 ggcgaggtgt tgcagcagta tggcgagttc gcccaaggcg ttgtcaaagc cgacggcaca
      781 cgccgccatt tcccgcgctt tggcggtcag ggctgcgccg tcttggttga tgatgcctgt
      841 cagcagttcg taaaggtatt gtttgtcaac cgcaccaatc atttggcgga catcttgttc
      901 ggcaactttg cccgaaccca gggcgatggc ttggtcgagc aggctcaagg catcgcgcat
      961 cgatccggcg gcggcgcgtc ccaaaagttg cagggcgggg ggggcgtagg cgattttttc
     1021 gctgtcgagg acgtgggcaa ggtggtcggc aacctgctgc gcggtcatat tgcgtaagac
     1081 gaattgcagg cagcggctta atacggtaac ggggactttg tgcggatcgg tcgtggcaag
     1141 gatgaatttg acgtgttcgg gcggctcttc cagcgttttg agcatagcgt tgaacgcgct
     1201 tttggaaagc atatgcactt cgtcgatgat atagactttg tatttgccgg cggtcggcgc
     1261 gtattgggcg ttttccaata cttcgcggat gttgtcgatg cctgtgttgg aggcggcgtc
     1321 gatttccagc aggtcgacat agcgtccggc atcgatctgc gtacagcttt ggcacacgcc
     1381 gcaaggttcg ccgtgttgcg cgttttcgca gttgaggctt ttggcaagga tgcgggcgat
     1441 ggtggttttg cctacgccgc gcgtgccggt cagcaggtag gcgtggtgca gcctgccttc
     1501 gtccaaggca ttttgcaggg ctttgacgac gtgttcctga ccgactaggt cggaaaaggt
     1561 tttgggccgc cattttcggg cgagaacttg ataggccatg tttttctctt ggtttcggtc
     1621 gtgatgtttc tgtcggtgcg tcggaatgcc gtctgaacgg cggtctcggg cggcgtattc
     1681 tagcactttc ggcttactgt ccgcgcaaga acagtgcgtc caattctttc aaagtcagtt
     1741 tgacccaagt cggcctgccg tggttgcact ggttgctgcg cggcgtattt tccatatcgc
     1801 gcagaagggc gttcatttcg ggcagggtga gccggcggcc ggcgcggatc gagccgtggc
     1861 aggacatagt ggcgaggatg cggttttcgt gttcttcgat ggtttggctg ctgccgactt
     1921 gggcgagttc gcccaatacg tcgcgggcga gggagacgac atcggatttg cccaacattg
     1981 cgggtgcggc gcggacggcg agggtgttgc cgcccatatc ggataattcc agcccgaagc
     2041 ctgccagcgt ttcggcataa tcggcaaggg cggcgcattc ttcgtgggac gcggcaaagg
     2101 ttacgggaat aagcaggcgt tggctttgca gcctgccgtt ttcctgccgt tggcgtttca
     2161 ttttctcgta gttgacgcgt tcggcggcag cgtgcatatc gatgagcaac aggctgtctt
     2221 cggcttgggc aagaatgtag atgccaagta attgggcaat ggcaaaaccg agcggcggca
     2281 gttcggattg ggacgggatg ccgtctgaaa gcggcgtatc tgtttgggga gcaggcgttt
     2341 cagacggcat attgccgaaa cgtgcctgct cgaatcggct taactcaagg tcgatgtcgt
     2401 cggttttttt gtagagttcg gcataggtgt ttattgccgc gcggctttcg cgcagggaca
     2461 ggctgcgttg ttgcggcgca tatgcggact gatagggcat gggcgcggtt ttgcctgatg
     2521 aaccaaaggc attgtgtgta tctgatttgt tgcctgtcgg gtagttggat gcgcgatcaa
     2581 acagattttc gctgtcgttt tcagacggca ttggggtgga gacaacgccg gtaatgtcat
     2641 gcaacacttc gcctgcgttg ctgacgcttt cggtcaagtc ggcgcgtgtg tcggcaaggg
     2701 ctttattgag cgtgtggaac acgagttggt gcacctgccg gctgtcgcgg aagcggattt
     2761 cggttttggt cgggtggacg ttgacatcca cggcttcggg cggcaggtcg aggaaaagga
     2821 cgaaagcggg ggtgagggcg ttgtgcaaaa cgtcgcggta tgcctgcttg acggcgtgga
     2881 gcatcacttt gtcgcggaca aaacgatggt tgacgaagca gtattgtttg tcggttttgc
     2941 ctttggcgaa agtcggcttg gcaatcgcgc catagagccg caatgcgccg ctgccgctgt
     3001 cgatttccaa agatgccgtc tgaaaatcat cgccgacaat ggcggcaatc cgttcgtgca
     3061 ggctttgttc agggagtttg aacacttgtt tgccgtcgcg tttgagcgag aaggcaatgt
     3121 gcggatgcgc cagcgcgagg cgttcgagca tagtggcgca gtgggcgtat tcggtggctt
     3181 cagacttgag gaacttgcgc cgcgcggggg tgttgaagaa gagttcggcg gcttcgatgg
     3241 tggtgccgac ggggtgggcg gcggcggtgg ggctgctgag tttgccgtct tcggctttga
     3301 cttgggttgc gtgcgaactg tcttcttgac ggctggtcag ggtcaggcgg ctgacggagg
     3361 cgatgcttgc caagccttcg ccgcgaaagc ccatgctggc aacgtgttct aaatcgttta
     3421 aggttttgat tttgctggta gcgtgtcggt ggagcgcaag ctcgatgtcg tccggatgaa
     3481 taccgctgcc gttgtcgctg acgcgaatca ggcggatgcc gccgcccgcc agctcgacat
     3541 caatcgccgt tgcgcctgca tcgatactgt tttcaacgat ttctttcaag gcgttggcag
     3601 ggcgttcgac cacttcgccg gcggcgattt ggttgacaag atggtcgggc agggcggcaa
     3661 tgcgtggcat aaggtaggct tccgttgcaa aaacgcttta ttctataata aaacccctta
     3721 cttttctgcc tgtattcttg ataaaagccg tttatcccgt ttggaaaata ccagtataat
     3781 cacgccgtat cccgtaaaat tggagcgcaa agatgtatca ctaccaatcc gaagccacac
     3841 aattcctcaa ccgcctgatt gaagaaaaac ctgagttgga acagcagcgt ttggaaaacc
     3901 gggggctttt gtgggatgtc gaactcaatc ccgaagaaca ggaaaacttc gaggcggcaa
     3961 aagtagcgaa aaaaccttat acctattacc aagactgaac ccgccgaaat gacgacccat
     4021 ctgtccaatg tcgcaccgga cctgcaaaac tatctgaaca gcatcggcga acccgaacat
     4081 cccgttttga cgcggctgcg tgagaagacc gagcatcacc gtatgggcaa aatggcgatt
     4141 gcgcgcgaac aggcggcggt tttggtttgg ctggcaaagc tgattcgcgc ggaaaaatat
     4201 ctggaagtcg gcgtgtttac cggatacagc agcaccgcgc ttgcattggc gttgccagaa
     4261 cacgggcgga ttaccgcctg cgacatcaat gtaaccttta ccgatacggc gcgtcaggtt
     4321 tggaacgagg caggtgtggc acataaaatc agcctgcacc tgcaacccgc attgctgaca
     4381 ttggatgatt tgattgcaca gggtgaagcc ggaagctacg atttggcact gatagacgca
     4441 gacaaaccgc ccacgccgcg atatttcgag cgttgcctca agcttgtccg tcaaggcggc
     4501 atcatcgcca tcgacaatat tttgctgaac ggaagggtga tgcgcgaagc ggcttccgat
     4561 gcgccgccca gcgtcggcat cctcaaagat ttcaatcaaa acctgccgaa cgatacgcgg
     4621 attgtcccca tcaccctgcc cgtcggcgac ggcttgaccc tgcttctgaa aaaataatga
     4681 agaccaaatt accgcttttt atcatttggc tgtccgtatc tgccgcctgt tcttcccctg
     4741 tttcccgcaa tattcaggat atgcggcccg aaccgcaggc agaggcaggt agttcggacg
     4801 ctattcccta tcccgttccc actctgcaag accgtttgga ttatctggaa ggcacactcg
     4861 tccgcctgtc gaacgaagtg gaaaccttaa acggcaaagt caaagcactg gagcatgcga
     4921 aaacacaccc ttccagcagg gcatacgtcc aaaaactcga cgaccgcaag ttgaaagagc
     4981 attacctcaa taccgaaggc ggcagcgcat ccgcacatac cgtcgaaacc gcacaaaacc
     5041 tctacaatca ggcactcaaa cactataaaa gcggcaggtt ttctgccgct gcctccctgt
     5101 tgaaaggcgc ggacggaggc gacggcggca gcatcgcgca acgcagtatg tatctgttgc
     5161 tgcaaagcag ggcgcgtatg ggcaactgcg aatccgtcat cgaaatcgga gggcgttacg
     5221 ccaaccgttt caaagacagc ccaaccgcgc ccgaagccat gttcaaaatc ggcgaatgcc
     5281 aatacaggtt gcagcagaaa gacattgcaa gggcgacttg gcgcagcctg atacaggctt
     5341 acccgagcag cccggcggca aaacgcgccg ccgcagccgt gcgcaaacga tagtttttcc
     5401 atttttccgc tttttacccc atccgcacca aggaggattt atgatacaaa tcggcatcat
     5461 catgggcagc aacagcgatt ggcccgttat gcggcaggca gcgcagtttc ttgaagagtt
     5521 cggcgtagaa tatgaggcgc gcgttgtttc cgcacaccgc accccggatt tgatgttcca
     5581 atacgccgaa accgcacggg cgcgcggcat caaagccatc atcgcgggtg cgggcggcgc
     5641 ggcgcattta cccggtatgg ttgccgccaa gaccaccgtc cccgttttgg gcgtacccgt
     5701 ccccagcaaa tacctgcgcg gcgaagattc gcttttatcg attgtacaaa tgcccaaagg
     5761 cgtacccgtc gccacattcg ccatcggcga ggcaggcgcg gcaaatgccg cattgttcgc
     5821 cgtttccatg ctcgccaacg aaacccccga actggcgcaa aaactggcag acttccgagc
     5881 caaacaggaa caggctgttt tgaatatgga attggaacaa atttaaaacc caccgtctgc
     5941 gaatatatag tggattaaat ttaaaccagt acggcgttac ctcgccttgc cgtactattt
     6001 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat acaaatgccg
     6061 tctgaagccc tgtttcgggt ttcagacggc atttttgcga acaacttttt atgcctgttc
     6121 tttttggcgc attttttctg ccattagttc gtgcaaggtt ttcttcgccg gtttcatcgg
     6181 cacgcggttt tgcgtccaac ccaactgttt gcgtggggtc aggttgcgga acttggtggc
     6241 tgcccaaccg aaggcgcggt aggttttgct gccgctgaaa ataccgttga atgtgcgcca
     6301 cgccatttgt tcgccgaagg tatgcgatgc gccttgccct cggatggggt gcggcacggt
     6361 ttcggtcggc gaacgttgcg cttcaacacg cagacgttgc atttgttcgg taatcggaat
     6421 gcgtaccgga caaacttcaa cgcacgcgcc acacatagta cacgcagtcg gcagatcgcg
     6481 tgtcgcatcc aagcccaaca aatgcggaga gataatctcg ccaatcgggc cgggataagt
     6541 cgtaccgtaa gccgcaccac cgatacgggt ataaaccgga cagtggttca tacacgcacc
     6601 gcaacggata cattgcaagg tgcggcgcat ttgatcttct gcgtaagctt ggctgcggcc
     6661 gttgtcgagc agaaccaagt gcatttcttg cggaccgtct aattcttcac tgcggcgcgg
     6721 gccggtaatc atattgaaat aggttgtgat gttttgaccg atggcggagc gcggcagcag
     6781 actgtacaag ggtgggatgt cggacaattt cgccaccact ttttcaatgc cggtaatagc
     6841 gatatgcacg ggcggtacgg tggtactcaa gcgaccgttg ccctcgtttt ccaccagaca
     6901 cagcgtacct gtttcagcaa cggcaaagtt tacgccactc aaaccgacat cggcagtgct
     6961 gtaaatatcg cgcagtgctt tacgggcgaa gccggtcagt tggtctacat cgtctgtcag
     7021 cggcgtaccg aggttttggt ggaacagttc gctaacctgt tctttggttt tgtggatagc
     7081 aggcatcacg atatgggtcg gtttttcacc tgccatttgg acgatgaact cgcccaagtc
     7141 gctttccacc gctttaatgc cttttgcttc aagataatgg ttcagctcga tttcctcgct
     7201 gaccatcgat ttgcctttga ccatcagctt gccgtttttg gctgtgatga tgtcgtggat
     7261 gatttcgcag gcttcggccg gtgtttccgc ccagtgtact tttacgccca acttggtcag
     7321 gttttcttcc agctgctcca gcagggcagg caatttagac aatgagcgct gacggacgtg
     7381 ttcgcacaaa tcgcgcaagc tttgcagctc ttcttcgtcg gtcaaaacag ctttgcgttt
     7441 ggtcatcagc atatccatcg cggtacgcag gcttttacgc aaaggcttgt cttgaaggga
     7501 aattgcggcg ttttgcttga aagtttccgg cttcatgtga aatttgatgg tttgcgtagt
     7561 catgcgtttt cctccaaatc ggtaggggaa atgtggtcgg gcaggatggc gaggataacc
     7621 aaatcacgcg gaccgtgcgc gccgtaagca agcgtcagtt ggatgtctgc ggttttggac
     7681 ggaccggaaa tcaggaatac attggtcggc ataccgtttt ccaccagttt ttcgccttcg
     7741 acggcattat gaaactcgtt gtacatcttg gacgtatcga acaggcagaa atgcacgggc
     7801 ggaacgagac tcaaagtacg cggctcttcg gggctggaaa acagcatcag cgtgccggtg
     7861 cgggcgatgc cgcattgcgc gccgctgaag cccgcatcga tgttcgtgaa aaactcggtt
     7921 ttccaagtat cgatttcgcg ctcgaaggca atcggttcga tattgctgtc cgccaatgcg
     7981 gcacgggcaa tttgtccgtg ttcggtcgcc aagggcagca ggatgttttt caaacccttg
     8041 ccttctgccg cttcgcggaa aacctgcatc caattgcttt tcgtcaccca ataaatttcg
     8101 gttttgaccg cgcgcattgc ggcagcccaa tgtttcagac gctcggcttc gctgccccaa
     8161 gaaacaccca tttcacggta ataatcaaaa accgcaggtt cttccatcgg caatgcgtcg
     8221 gcttttttca gttttgccaa aatattttcg cgcgcgctca tgctttgcct ccggtacgtt
     8281 ccaacaagaa ggatgcgata tgtttcggac gcggcatatt cggttcgtcc ttagcaattt
     8341 tgccgccgat gttcatcata cagccgcagt ccgcgctgat gatttcggtt gcgccggttt
     8401 ctttcagcgc ggcgactttg tcggttacca tcgcgccgga aatatcggct tgtttgacgg
     8461 agaatgtgcc gccgaagccg caacattcgc tttcgtggtc gtggacgatg cgttcgacgt
     8521 tttccatacc gtcaatcagt tgccagcccg agagatggac attcatttcg cggcgggcgg
     8581 cgcaggaagt gtgaacggcg actttgagcg gttcgccctt gtcttcgggt ttgaaaccga
     8641 tggcaagcag gaaatgggta aactcgatga tgcggccggc gcaatccaca gccctttcct
     8701 cgtactcgct gcctttaaac agcgtcggcc aatggtgctt catcataccg ccgcacgaac
     8761 cggacggtac gacaatcggc cactcctcgg ggaaaaggtc gagttgcgct ctggcgacat
     8821 caaaggcttc ggtcggatgg ccggatgaat aggcgggctg accgcagcag ctttgcgcca
     8881 tcgggaaatg gacgcgtatg ccctgttgct cgattagggt aatggcatcc atgcctgctt
     8941 cgggcataaa aagatcaagg acgcaagtgc cgaaaaaata gacatcggtc ggattgctgt
     9001 cgtatcggat gatttttggg ggaatggttg cgctcatgtt ttttgataac gggaaacccg
     9061 tttttttctg tagaaaggta agcgtttact ttaagtaatt gactgttgcg ggtcaagtct
     9121 aattttaaaa aataatccgg tttttcttac aaactgcccc ataacgctta ctgtacctta
     9181 ctctgataat tttcgataat aattatcatt acaatgaaat gccgattcgt ttgcttgtga
     9241 acattcagga cgccgactct gacggcattc agacagcatc tgaaaacaat aacggcacaa
     9301 ggatggggta caatcgccct atggaaaaaa acaccctatc cgcccgcgca ccatcccctt
     9361 ggctgccgct tctgctggca attgccattt ttatgcagat gttggatgcg accattttaa
     9421 ataccgcact gcctgaaatt gccaccgacc tggacgaatc gcctctggat atgcaactgg
     9481 cagtcgttgc ctatacgctg acggttgccc tgctgattcc tttgagcggt tatttggcgg
     9541 acaggttcgg aacgaaaaaa gtctttttcg gttcgattgc cgtttttata ctcggatcgg
     9601 cattgtgcgc cgcatcgggt tcgctgtttg aattgacgct ttcccgtgtc gttcagggca
     9661 tcggcggttc gatgctggtt ccgataccgc gtctgaccat cttgcgtatg tacgacaagt
     9721 ccaagctgct caatgccatc aattatgcgg tcatgcctgc attaatcggg ccggttttag
     9781 ggcctttggc gggcggttat ttggtcgaat acgcttcgtg gcactggatt ttcctgctca
     9841 acctgcccat cggtctgctg ggtttcatat tgggacgcaa catcatgccc gatattaaag
     9901 gcagcaatat ctctttagac ttcaaaggct atctgatttt ttctgccgcc gcgtgcctct
     9961 tgttactttc ggcagaaagc ctgtcgcacg cgctgcctcc gtattttgca ctgttgccgc
    10021 tgtgcggcgg actgctgttt gcacgccgtt atttccgaca tatgaaaacc gcgtccaaac
    10081 cgatttattc cgccgacctg tttctgatac gcactttccg tctaggactg gcgggcaatc
    10141 tgttcagccg tctcggcatc agctcgattc cttttctgat gcccctgatg tttcaagtcg
    10201 ctttcggctt cggcgcaagc ctgtcgggtt ggctggtcgc ccccgtcgcc ctgtcttcgc
    10261 tgctggtcaa accgctgatt gcaccgctca tgaaacgttt cggctaccgc acggtactgc
    10321 tttggaacac caagctgctt gccgccttca tcatgctgct cgccctgcct gacggaaact
    10381 cgtcgctgtg gatttggatt ttcctctcgc tggcgatcgg cgcgtgcaac tccctgcagt
    10441 tttctgccat gaatacactg accctcgccg atttgcgccc gcaacagacc ggcagcggca
    10501 acagcctgat ggcggtcaac caacagcttg ccatcagcat gggcattgtt gtcggcgcat
    10561 taatccttaa aaactggaca tttctgatac cggcttcttc aggtctgcat tccgccttcc
    10621 gtatgaccct gctcagcatc ggcggcatca cccttgcctc gtcgttggtt ttcaagcgtc
    10681 tgcacgtttc agacggcgca aacctgacac ggaacacact gtcctaaagc ggtccgcacg
    10741 caaaactttt acccgtttca acgtttggat tatgattccg cacatccatg cgcgccaacc
    10801 ccaaaacaca ggcaatgccg tctgaaacca tatccctgat gaaaacacgc agcctaattt
    10861 cccttttatg cctccttctc tgttcatgtt cttcatggtt gcccccactg gaagaacgga
    10921 cggaaagccg tcatttcaat acttccaaac ccgtccgcct ggacaacatc ctgcaaatcc
    10981 ggcacacccc tcataccaac gggctatccg atatctatct gctgaacgac ccccacgaag
    11041 cctttgccgc ccgcgccgcc cttatcgaat ctgccgaaca cagcctcgat ttgcaatact
    11101 acatctggcg caacgacatt tccggccgac tgctgttcaa cctcgtgtac cttgccgcag
    11161 aacgcggtgt gcgcgtacgc ctgctgttgg acgacaacaa cacgcgcgga ttggacgacc
    11221 tcctgctcgc cctcgacagc catcccaata tcgaagtgcg cctgttcaac cccttcgtct
    11281 tacgaaaatg gcgcgcactc ggctacctga ccgacttccc ccgcctcaac cgccgcatgc
    11341 acaacaaatc ctttaccgcc gacaaccgcg ccaccatact cggcggacgc aatatcggcg
    11401 acgaatactt caaagtcggt gaggacaccg ttttcgccga cctggacatc ctcgccaccg
    11461 gcagcgtcgt cggcgaagta tcgcacgact tcgaccgcta ctgggcaagc cattccgccc
    11521 acaacgccac gcgcatcatc cgcagcggca acatcggcaa gggtcttcaa gcactcggat
    11581 acaacgacga aacgtccaga cacgcgctcc tgcgctaccg cgaaaccgtc gaacagtcgc
    11641 ccctctacca aaaaatacag gcaggacgca tcgactggca gagcgtccaa acccgcctca
    11701 tcagcgacga ccctgcaaaa ggactcgacc gcgaccgccg caaaccgccg attgccgggc
    11761 ggctgcaaga cgcgctcaaa cagcccgaaa aaagcgtcta tctggtttca ccctatttcg
    11821 tccccacaaa atccggcaca gacgcactag caaaactggt gcaggacggc atagacgtta
    11881 ctgtcctgac caactcgcta caggcgaccg acgttgccgc cgtccattcc ggctatgtca
    11941 aataccgaaa accgctgctc aaagccggca tcaaactcta cgagctgcaa cccaaccatg
    12001 ccgtccctgc cacaaaagac aaaggcctga ccggcagctc cgtaaccagc ctgcacgcca
    12061 aaaccttcat tgtggacggc aaacgcatct tcatcggttc gttcaacctc gacccccgtt
    12121 ccgcgcgtct caacaccgaa atgggcgttg ttatcgaaag ccccaaaatc gcagaacaga
    12181 tggagcgcac ccttgccgat accacccccg aatacgccta ccgcgttacc ctcgacaggc
    12241 acaaccgcct gcaatggcac gatcccgcca cccgaaaaac ctacccgaac gaacccgaag
    12301 ccaaactttg gaaacgcatc gccgcaaaaa tcctatccct gctgcccata gaaagtttat
    12361 tatagaaata tagcggatta acaaaaacc
//
LOCUS       DAWHXS010000061        12264 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWHXS010000061 DAWHXS010000000
VERSION     DAWHXS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.061"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(71..487)
                     /locus_tag="WIE22_001316"
     CDS             complement(71..487)
                     /locus_tag="WIE22_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127394.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            complement(484..675)
                     /locus_tag="WIE22_001317"
     CDS             complement(484..675)
                     /locus_tag="WIE22_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127395.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            complement(719..961)
                     /locus_tag="WIE22_001318"
     CDS             complement(719..961)
                     /locus_tag="WIE22_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127396.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            1198..1365
                     /locus_tag="WIE22_001319"
     CDS             1198..1365
                     /locus_tag="WIE22_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127397.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1638..1823
                     /locus_tag="WIE22_001320"
     CDS             1638..1823
                     /locus_tag="WIE22_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127398.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            2024..2224
                     /locus_tag="WIE22_001321"
     CDS             2024..2224
                     /locus_tag="WIE22_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127399.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            2356..2964
                     /locus_tag="WIE22_001322"
     CDS             2356..2964
                     /locus_tag="WIE22_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ4127400.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIS
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            2968..3183
                     /locus_tag="WIE22_001323"
     CDS             2968..3183
                     /locus_tag="WIE22_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127401.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(3344..3862)
                     /locus_tag="WIE22_001324"
     CDS             complement(3344..3862)
                     /locus_tag="WIE22_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ4127402.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(3972..4139)
                     /locus_tag="WIE22_001325"
     CDS             complement(3972..4139)
                     /locus_tag="WIE22_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127403.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(4132..4305)
                     /locus_tag="WIE22_001326"
     CDS             complement(4132..4305)
                     /locus_tag="WIE22_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127404.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATKFMVKRNREQEVAEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(4414..4680)
                     /locus_tag="WIE22_001327"
     CDS             complement(4414..4680)
                     /locus_tag="WIE22_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127405.1"
                     /translation="MQVLKKVDWKMFVARSFWRWVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            complement(4770..5684)
                     /locus_tag="WIE22_001328"
     CDS             complement(4770..5684)
                     /locus_tag="WIE22_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ4127406.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMEDSKARREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQSVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            complement(5720..7696)
                     /locus_tag="WIE22_001329"
     CDS             complement(5720..7696)
                     /locus_tag="WIE22_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4127407.1"
                     /translation="MKYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     NARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAESKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIVGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAAW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFETDRS"
     gene            complement(7698..7949)
                     /locus_tag="WIE22_001330"
     CDS             complement(7698..7949)
                     /locus_tag="WIE22_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4127408.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            7993..8892
                     /locus_tag="WIE22_001331"
     CDS             7993..8892
                     /locus_tag="WIE22_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ4127409.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            9247..9864
                     /locus_tag="WIE22_001332"
     CDS             9247..9864
                     /locus_tag="WIE22_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4127410.1"
                     /translation="MVWPEYSPQENVALFRHNGEILLSGINIKLKNGNSLLIRGPSGC
                     GKTSLLRALAGLWPFGSSGKVSHPPHQDILFLPQRPYTAQGSLRDAICYPAIDKQHPE
                     LIEAMNTCRLGYLVDKLDKTDDWQHKLSPGELQRVAFVRALLSQPKVILLDEATAALD
                     EPTEAMLYRALKQKLPDSIIISIGHRSTLNAFHNMHLDVGNVACG"
     gene            9968..10636
                     /gene="hda"
                     /locus_tag="WIE22_001333"
     CDS             9968..10636
                     /gene="hda"
                     /locus_tag="WIE22_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ4127411.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            10633..11301
                     /locus_tag="WIE22_001334"
     CDS             10633..11301
                     /locus_tag="WIE22_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4127412.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAKYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSRNDLPLLRLVSEPVAANPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         3307 a         3237 c         2480 g         3240 t
ORIGIN      
        1 aaaaatgccg tctgaaaacg agctttgcga gtttcgccaa aacgtttcag acgacctttt
       61 ttactacaat ctaatcactg tctttttcgc gccgttgctt ttccaaggtt tgcgctttac
      121 ctctgttgat tcctgcaaaa tatcccaaag ctaccgcccc gccgattttg acaatttcaa
      181 tagcgacgct gtccttatcg cgccataacg cagtaatcag cacggcggca atcagaaaag
      241 aaatagcaat cacggcattc agacggcgtt tgtgtacctc tttgaaaatc tcaccatgtt
      301 gctgttcacc ctgtttttgc gcttcgatgg tagccagtgc aatgcgttcg tgagattgga
      361 tttcgtcctt acggctttcc aactccttct gtttgactgc aatatcctgt ttttgaagtt
      421 ctaaaaactc ctgaagcatt ttctgctgct catcggccgg cagattctcg gatttttccc
      481 cgctcataaa tcaaccgcca ctaaggtttt gtaacggact ttgaatttgc cgtaaccccg
      541 agcccctatt ttcggcggca caaattccac ggacgcaata ttgcccgtct tacggtcttt
      601 acggacttct tccaaataat cggcagcatt gagcagccgt tcgcggactg ccgtatattc
      661 tttgacggta ttcatttttc aacctcgccc aaatagtgca aatattagca tatgggattt
      721 atttttcaag aaaatcaaat ccatataaaa caaccgtata atattttaat actatatatg
      781 gtaaatttct tctaaaaata cacactaaaa taccatatct tgataaccgc aacgccgcac
      841 aaatggcagg gcattgatgc ccgacaacaa aaaatgccgt ctgaaacagc attcgagctg
      901 ttttcagacg gcatttttct ttgattttgt tggggaaagg gttgactttt aacgtgtcca
      961 cgtttataat tcaattcatc ggcgtagtga gggccgatac gggaaaagcc ccaccgaagc
     1021 ggggctcaac tgggagaaag aagatgaata aacgcactat tcaaattctc atcttggtcg
     1081 cagttttcat gttggcaagt gctaatgcct actaaagact gaccagctgg gggcggtcgc
     1141 cgctgtcccc agtcccaatc ttacaaaata cggtttaaaa aatcaaggag tttgcctatg
     1201 gttgatgaaa aactggcaga aaaccgaaag cgttacgagc agaaacgggt gattaagaaa
     1261 gtctcgttca atgccgaaac ggaaaaagaa cttttggaat atgcccaaaa tatcgatttt
     1321 tcccaatggg ttaaatcaat catcaaagaa aaaatcaaaa aatagcttga ctaaattcta
     1381 gaaatactag ataatacgcc tatctgataa agcaaagccc cgaagcggca acttcggggg
     1441 cttatcggat agaaaggagg cttgtggaaa tgacaaaact ggcgaaagtt gtttttttca
     1501 tcattctgct tttaagctgt agtccggctt actaagcaag ttgattaaac acaaggggtg
     1561 gcagaacacc gcgccccttt cgccaacctt tctgcaagcc attttactac aacgatttta
     1621 aaaatcaagg attgcctatg cctgatgaaa aaacgctaaa agcaaaacaa gctgaatatc
     1681 agcgaaagta tgaccaaaag cggctgatga aaacagtttc tttccatttg gaaagagaga
     1741 aagagttggt ggattttatg caaaaagaaa ttcccgactt ttcaaactgg gttaaatcag
     1801 tcatgaagga aaaaatgaaa taagaaaatt ccgccgatgg gaaaacatcg gcggaacagg
     1861 tggaaagcag agcagcaact ctctttatgt agggcatgaa aaaaggccgt ctgaaacagg
     1921 ttttaaaccc catttcagac ggctttttgt tgtaatacat cttgattaaa atggttttta
     1981 gtattaccat aatcgtgttt taaaacctat ttaaaggaag attatggact cgattaaaaa
     2041 ggctcaagaa aaatacgcac tcaagcgagt agtgaaaaca gtatcgttca attccgaaaa
     2101 agaacttgat ttaattaaat tttctgaaaa tttagatttt tcaaactggg taaaatcgaa
     2161 aattagagga gatatggaat cggaaaaatt caaaaaaaaa gcaaaaaaga atattgacaa
     2221 atagaattac cataatttaa aatttccata tcttgtgcaa cacaagatat ggaaatcccc
     2281 gataagtgac agcttatcgg ggatagtgga aaagcaacgt tagagcgttc ttttccttgt
     2341 catctcaacc ttgaaatgaa agataatttt accatgaata cccaactcat cccaactgtt
     2401 tcaggtcaat tggacaatca aacacaagca ctggttgatg ctcatgacct gcataagttc
     2461 ttaggtgtgg aaacaccatt ttctaaatgg attcagcgtc gcattgaaga atacggattt
     2521 acacaagccc ttgattttat cggcgtggac aaaattgtcc gcaccgaagc aggtttcttt
     2581 ggacagcgtg ataaaactgt tcaggggtat tatttatccc tcgacatggc aaaagagctg
     2641 tgcatggtgg agcgcaacga caaaggacgc caagcccgcc gatattttat cgaaatggag
     2701 aaacaggcaa aagccctgcc tgatgccgtg ctataccgca tcgatgcatt ggaagacgcc
     2761 tatttccaag ccgcgcccga aatgctcgcg ctgctgcgct accgcagtat gggcttgaac
     2821 ctgaccgaaa taagcaaact cttggacatg aaccccggcg cggtttccta ccgtctcaaa
     2881 aaactcaacg atttgggttt cttggaatat gtccccaagc ccaaaatggt ggtgcaacaa
     2941 cagtctttgg gattggaggg ttaagccatg aataccacct ataccctgac tttcgaccaa
     3001 gacagcctca aagccctgaa tctgctggtg ttcacactca accatctgaa agttttggag
     3061 atggacatgg aaggaatcga agacggtttg tctgccgtga ttgaaaccgc ctccgagaaa
     3121 gccgacaagc tgtccgccgc tttctacaac gccgtgtacg agcaggaaga gaaagcagcc
     3181 tgaatctagg gcatgaaaaa aggccgtctg aaacaggttt taaaccccgt ttcagacgac
     3241 ctttaaatca tgggttaaag cctatttgaa ccggctaggc aagctcctgc tccgtaggct
     3301 caatcacgtt tcaacacact gaattgaaat caggctgttg attttataga atttgttcgc
     3361 tcggctcaat cacaaaatcc tccacgccgc tcacaatctt aatccccggc acttggccgt
     3421 ctgaaaaacg ctctttttga ttcaggatgg cgtctttgtc gatttccttt ttagtgcgga
     3481 caaactcggc aaaggcggat ttttccgaga gccacgccaa gacggcggcc acgcccgtta
     3541 ccttgacgga tggcggacgg atgcgccatt taatcaggcc ggtagtaaag tccacggttt
     3601 tggttttacc gttttccgtc agctcgtcct tatgtgcctc gcagtatgcg gccacacgtt
     3661 cggtcaggct catgatttcg gcacacatcg gcgcggcttg ggcggcatat tcttcttcga
     3721 tggccgcttt tttgtctccg gcttcggttt ccaggcgttt gacttcgcgc tgcaagtcgc
     3781 cgatttggcg gataaatgca gtaacttccg ttttgtcttg tgccgcttcg atggcgggct
     3841 gtttgattcg ggttttagcc attttctctt cctttcaggt tgggtttaac atttcggctt
     3901 actcaagcct tgttcgcgtt cgtgcttggc aagtgcttca actttcgcgc ggtttctcaa
     3961 aaacttaaac atcacacttc ctcccataaa gttatcgcct gagccaaagt ttccgcctcg
     4021 gcagtcttcc acatcccgtc cggcgcacgc gctgcaatca caaagccttc gccgtccttc
     4081 ttcatgacca tcagctcgcc acggtcttcg agccattcga ttaaatcttt ttcattcatc
     4141 tttgtttcct tccgttttca gacgaccttt gccgtcctta tcttcaaact gcgcctgata
     4201 ttcggcgact tcctgctcgc ggtttcgctt caccataaac ttcgtcgcac gcctgcggtg
     4261 ttgtccccaa gcctgccaat ccgtattacg tcgtccgaaa ctcatttcac acatcctttc
     4321 acaatcgcct tatcgccata tttcgcgcgg atttccttta ccgcccgtgc caaagcctct
     4381 tttttcgccg cagggctcaa taaaattggc ttatcactca taaacaatcc ctttcatttt
     4441 ttcctcgacg ctcattgact cgtattgccc ttcccatacc ttgacctcca aatccgcctg
     4501 tctttccatc gcctcaacct tcgtcggctc tttcgcaacc ggttcgggtt ctttcgcgca
     4561 ggaatgcaac gccattcccg ccacaaaaca ccacacgccc accgtcaaac caaccggcac
     4621 ccaacgccaa aaagagcgcg ccacaaacat cttccaatca actttcttca aaacttgcat
     4681 tttgcgtttt ccttttaaaa caacaactta ttaaaatcat agggtaaaaa aatatatagc
     4741 cctgtcagag acttaccgtt tcagacggcc tatcggataa tcagggagct gtatttctta
     4801 acgataccgg cctgcatctt gataccgttc ttgttcgccg tgcgtaccgc gccgcgcatc
     4861 aatttactca tccggcgcgt attgccgtta ctatgtttaa ccagttccaa gagcgtttct
     4921 tcgtccgcat caggcaaagc cgctttcgca atctcaaaga gttcgtcatc cggcaaagat
     4981 tcgcccaaat tcagcgcaac agacacgcgg ctataaagtt gcaccagctc gccatgctta
     5041 ccgcgcaaat tcgcaaccag tcggggcata ccgctcaaca ccaaaccgca gcccgtttca
     5101 tcatgcagtc ggcggataat ctcaagggcg cgcaagggca ggttttccgc ttcatcgacc
     5161 acaatcagac ggcccgaatc gcgcaggcgg tcagatacag actcaaacaa atcattcagg
     5221 ctgcccatcg ccgatacctt cgccgccgtc gccaacttgc gcatcagtac caaagccgta
     5281 aagctcggat tagcctcaat caagatagcc gcagggtttt tctcgcagta gtttttgacc
     5341 gactgcgtct tgcccaaacc cgcctgaccg tagatcacca ccgtttcgcc gccttcgtgc
     5401 gcatcgcgca tcacttccgc gattcggcgt gtcgtcttag tcgatacaaa tcccaacacc
     5461 agctcttcac gtcgcgcctt actgtcctcc atctccaaaa acgcctcgat tttcgtctcg
     5521 atggtttcat aattgccgcc tttatcggca taagtgccgt tcagatacat actgatggag
     5581 gccggcgaag tatcgatacc gcgtgccagt tgggtttggt tcattcccga tttttcttta
     5641 aattcaacca gtttttgttg caatgcttga ttaatttgtt tcattttttt agtcctttta
     5701 aaagaggttt aaaaccgttt taacttctat ccgtctcaaa tagcacaaaa tcgtccgtgc
     5761 ccgttttcgg caataccgca tactccgcct cgatgacgtt tccgcccaaa tgtcccagct
     5821 cgtcccaagc tgccgcctgt tccagtgccg gattgacttc cgcatttgcg agcttgattg
     5881 cattttccgc ccgcttgatt ttgccttttc ggcggttttc cgccagttgg tcgatacgcg
     5941 ccgtcgggaa agcctcgcgg gtattgccgt cggcctgcgc cttagtgata aacttgccgt
     6001 ccatatcaaa cacattgacc accgacgcat cgctcaaatc gtagctgacc cgtacctcgt
     6061 ccttgtgata ctccgccagc tcggttgaga aataagagtt gttgaacaaa tccagccaac
     6121 cgcgctgtac ctttcgcacc tcttgcggca taaacatcgt cgccagctct tgcgccgaca
     6181 acatatccgg cgcgataccg tcctgttcca gcctcatttc ccgataagcc ttcggcgtat
     6241 aatgcccgcc gtcaggatgt cggggcagct cgccgtgcgg gcggttgttg tattcgtcga
     6301 tacacttgac cacatccgcg ataaaacgcg accagctcgg cagttttttc aaatatttct
     6361 gttgttcctc cgttaaatcc ttgccttttt ccaaagcatt aaacgcactt tccatcttgc
     6421 ggtacatcag gttcttcgta ctgctgtcca ttcctgcgcc cgcaaacgtc tcatactggc
     6481 gcgccatctc aatcagattg tctttccacc atcgctcaat gatgccgcgt ccttgcgggt
     6541 tgcccacgat acccgtttca tgccggatac ccaatcggga cgtaatacct gtgatttcat
     6601 ggtctatcgt cttgccggtt tggccgccgc cgttatccga gtaatagata atcggcaaac
     6661 caaaatgctt gaccccgata cgcagagcgt ccgataccgc cacacaactt tcagcaagag
     6721 agaccgaaaa cccgaccaca aaccgcgtac aaccatcaat aatcaccgtc acttccggct
     6781 taaacggcct gccgtgtaca gggtgcgcca ccttcgcctt aaagctgtgg ccgtcgccga
     6841 tccaaacatc gttcggcttc aaagcccccc aatcacgttt cacataaggc agcagcgatt
     6901 tataagccgc ccccgttttc ctgccgcgct cctgcataat cagcggcagc ttgtcccaaa
     6961 cgcgacgcac catactcaaa ttaggcacat cattgaccgg catattttcc gcttcagccc
     7021 actgcacaaa tcggcggtaa ctgtgcgcca gcttcggagc tgaagggatg ttgtgaaact
     7081 gcataaacat cggcaaccaa ccgtagctct caatcggctt gaccgccttc gtcgtcttcg
     7141 gagccaaagc aaccaaccgc tccgtcgcgt tttccgcttt caaataagca gaaatccagc
     7201 cgtctaaagt acgttcgcca acctttgccg tccggctgcg gtcattggcc ttttccaagt
     7261 tcccaagcgt gaccgcgtcc aatttacttt ctgccagcaa gcccaaaaac tgagccaccg
     7321 cagcctttgc agagcaaccg tattggtatt taatccccaa taccgccgcc accaccgcac
     7381 atcgcgcatt cgccaccgac cgttgtttct cgttcaacag cttggccgct tcagccagtg
     7441 cctgagccga catcgccgtc cccggtctga cttggggcag cattttcggc atcttctccg
     7501 ccagctcgtc cgactgccgt ttcatgatgg ctgctcggat ttcggcaggg agggcatatt
     7561 caacaccacc acccttacct tgacgccttc tcgtttgcca accatccctt tgcgcttttt
     7621 ttctcagccc tattactgtt cttggcatat ttggcaaccc aaaattaagc aggcttttga
     7681 ggtccatata tttcatttca acccactccg agcatctctt tcaattcagc tatctttttg
     7741 tctcgacaat ctgttttctt gtaatgcgga aaaacttcaa ctaaatcttt ttgaatcact
     7801 ttcgcgattg cttccgcaat tcttttgctt cctcgtccat ttcttatgac ctcagaaaca
     7861 gattggttag ttacccctaa agccgaagcc aacattcttg ccgatatatt tttttcttct
     7921 aaggatttat agatttcgtt cgcttgcata atgcctccat gatagaatcc attacttacc
     7981 ttacaggctt taatgcctgc cttaactgtt acttggatga tatacacaaa atatttggta
     8041 taccaaatat atttggtaat attttgcgat gtttttatgg aacgcattga tttagcattg
     8101 aatttagttg aagagagaag ccgacttgga tactctcgaa agaattttgc tgagcaaact
     8161 ggatgcagtg cagaaagttt gagactgtat gaaagcgggc aggtcaacat tcctgcggac
     8221 tttttacttg ctgctgtcca attaggggtg gatattcaat ttgtctttac aggcattcat
     8281 tcggcaaatt taaatgctgt ttcagacgac cttaataaaa aattggacga cgctattggc
     8341 aaatcaaacg ttgagaatca gattaacggc aacgtcagta acgctgttat cgcctcatct
     8401 ggttcggttg ttcatcaaat taatacccaa aatcacgtca tcagaacccg tgtcgattca
     8461 aaacccggca aggaacatat aacgcttgag caagcttcaa agctgcaaca acttgttaaa
     8521 caagttgccg cagcagaaga gattgccaaa cggtcgccaa aatcaatccg tgcaatttgg
     8581 gctagtctga atgctcactg taaagttcca agttacaaac taatcgcatt gagcgattac
     8641 gacaaagcag aaacttatct cagaaaatgg ctgggtaggt tgagcaatac agcaacatca
     8701 aagaacaacg atcccgactg gcgaaaaaag aaatacgctt atatcaaatt aaatgtaaaa
     8761 cagcttgagc ttgaagattg gttgaaaagt tacctcgaaa aaaatttctc agtagaaagc
     8821 ctgactgaat taagtgatga agatttgcaa aaaacatatg cagcagtatc gactaaaaaa
     8881 cggaaaaaat aggcttaaac atgaaactcc gcctttaaat tcaagtttca tgtttagctt
     8941 atggtcttca gatgctttgt ttttttagat gtatgatatt aaaagatttt ttcaaacatg
     9001 gaactattat acaagttcca tgttcagttt ctagttaaag ccttttataa acattctaat
     9061 caaccacgcc gaaaccgagc cgcgcgacgg cctatatgtc ttgcgtatag gcaatgacct
     9121 ctttgtcaaa cgtgtgcaac gtataccggg caaactgctt gtaacgtctg aaaatccacg
     9181 ttacgcaccg tttgaaatag atttgagtaa tacccaagac gacatcgcca tcataggccg
     9241 tgttgagtgg tatggccgga gtattcccca caggaaaacg tcgctctgtt ccgccacaac
     9301 ggcgaaatcc tgttgagcgg catcaacatc aaactcaaaa acggcaactc cctactgata
     9361 cgcggcccga gcggttgcgg caaaacttcg ttgctgcgcg cgctggcggg gctttggccg
     9421 ttcggcagca gcggcaaagt cagccatccg ccgcatcaag acatcctctt cctgccgcaa
     9481 cgtccgtaca cggcacaagg cagtctgcgc gatgcgattt gttaccctgc catcgacaaa
     9541 caacatccgg aactgatcga agccatgaat acctgccgat taggttatct ggttgataaa
     9601 ttagataaaa ccgatgactg gcaacacaaa ctctccccag gcgaactgca acgtgttgcc
     9661 ttcgtccgcg ccctactctc gcagcccaaa gtcatcttgc tcgatgaagc taccgccgct
     9721 ttggacgaac cgaccgaagc catgctctac cgcgccttaa agcaaaaact gcccgacagc
     9781 atcatcatca gtatcggcca ccgcagcacg ctcaacgcgt tccacaatat gcacctcgat
     9841 gtcggcaacg ttgcctgcgg ctgaacgatg ccgtctgaag ccgttttcag acggcatcgt
     9901 caaattggaa aggcgcgcga aaacaacgta caataaacct atctgccaac ctctgaaatg
     9961 ctgcaccgtg aaccagctta tttttgattt tgccgcacac gactatccaa gtttcgacaa
    10021 attcctcggc acggaaaatg cggagctggt ctatgtcctc caacacaaac acggacagtt
    10081 tatctatgtc tggggtgaag aaggcgcggg aaaaagccac cttttgcagg catgggtcgc
    10141 acaggcactc gaagccggca aaaacgccgc ctatatcgat gccgcctcca tgccgctgac
    10201 cgatgccgcg ttcgaggcgg aataccttgc cgtcgatcaa gtcgaaaaac tgggcaacga
    10261 agaacaagcc ctgctttttt ccatcttcaa ccgcttccgc aacagcggca aaggcttttt
    10321 gctgctcggt tcggaataca cgccccagca gcttgtcatc cgcgaagatt tgcggacgcg
    10381 tatggcatac tgcctcgttt acgaagttaa gcccctaacc gaccaagaaa aaatcgacgc
    10441 gctcgccagt atggcggcgg cacgccaagt aaccgttgat tccgaaattt tcgaatacct
    10501 gctcaaacac tggcggcgcg atatggacag cctgatgatg atgctcgaca cgctggacaa
    10561 ctacgccgtc acgatgggca aacgcatcac cctgccgctt ttgcgccagc ttttgaaaca
    10621 acaggaaacc caatgaaaaa cctcgccatc ttcgacctcg acaacaccct catcaacacc
    10681 gattccgacc acgcctggcc gcaatacctc atcaaaaaag gcttggttga cgctgccgaa
    10741 accgaagcgc aaaacgaaaa attctaccgc gactaccaaa acggctgcct cgacatcgac
    10801 gcattcctca aattccacct cgccccgctc gcccgctaca gcaaagaaga gctggcggaa
    10861 tttcaccgcg aatttatggc gaaatacatc atcccccaca tctcgcctat gcagcgtatg
    10921 ctggtgcaga gccaccaaat ggcaggcgac gaaaccctcg tcatctcctc aaccaacgag
    10981 ttcatcatca cccccgtctg ccaccttttc ggcatcacca acatcatcgg cacacaactc
    11041 gaaaccggtt ctgacggccg ctacaccggc aattacatcg gcacgcccag cctcaaagaa
    11101 ggcaaaatta cccgcctgaa ccaatggctt gccgaacgcg gcgaaacgct ccaaagctac
    11161 ggcaaaacct atttttacag cgactccaga aacgacctgc cgctgctgcg cctcgtcagc
    11221 gaacccgtcg ccgccaaccc cgatgccgag ctggaaaaag aagccaaaga aaaaggctgg
    11281 ccggttttga atttcaaatg acgcaatgcc gtctgaagcc cgattcggcg ttcagacggc
    11341 attggggctg aaatcccaaa aactcaaagc cacaggaatt tatccggaaa aacaaaaaac
    11401 acaggaattc atcgggaaaa acaacaatct ttccaccgtc attcccacga aagtgggaat
    11461 cttgatccgt ccgcacggaa acttatattt cgtcatttgc acccgacata attcaatact
    11521 ttattttgca ataaattaac ggatgctgac aaattagatt ggattaatta ccctattatc
    11581 catattgggt tatcgggttg ctaattgagc agagcgtagc aaaccataaa gtgagtgttt
    11641 ccacccactc tgcatttact atgaagtggt tattaaatat gataatgaga cctattaaat
    11701 ttagtattgt aaatacgtta ttatttattg ttatatgtag ttcatttttt gatctgctcg
    11761 ttcaattatg tacaatttta tttcatagcc aaaaaatata ctttattaca ttatttttat
    11821 tatttatttt taattttgtt acaaaatcta tctatatggc aattatttat cctattttat
    11881 atttttttac gataaaaaaa atattatcct tactctagga aagtgataat tctattatca
    11941 ttagcattat ctatatattt tagttttatg gacttttact ttttttccat atattcagat
    12001 aaccttagct atgaaacgga gcctttacat ttatacatcc ctattattat taattttttc
    12061 tcacttttag tttctaattt tattttatct tttatcaaca agtaaatgta tatacaaact
    12121 acaatccaaa agatcctaag caaaccacca atcccacacc acccttaccg gttcaaatcc
    12181 gcacaaaaac ccacatcccg tcattcccac gaaagtggga atctaggacg tatagtggat
    12241 taacaaaaat caggacaagg cgac
//
LOCUS       DAWHXS010000062        12151 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWHXS010000062 DAWHXS010000000
VERSION     DAWHXS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.062"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            141..575
                     /locus_tag="WIE22_001335"
     CDS             141..575
                     /locus_tag="WIE22_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ4127413.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(623..1966)
                     /locus_tag="WIE22_001336"
     CDS             complement(623..1966)
                     /locus_tag="WIE22_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ4127414.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1971..2624)
                     /locus_tag="WIE22_001337"
     CDS             complement(1971..2624)
                     /locus_tag="WIE22_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ4127415.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2775..3065
                     /gene="gatC"
                     /locus_tag="WIE22_001338"
     CDS             2775..3065
                     /gene="gatC"
                     /locus_tag="WIE22_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ4127416.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3128..4573
                     /gene="gatA"
                     /locus_tag="WIE22_001339"
     CDS             3128..4573
                     /gene="gatA"
                     /locus_tag="WIE22_001339"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ4127417.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4570..5502
                     /locus_tag="WIE22_001340"
     CDS             4570..5502
                     /locus_tag="WIE22_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ4127418.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5545..6975
                     /gene="gatB"
                     /locus_tag="WIE22_001341"
     CDS             5545..6975
                     /gene="gatB"
                     /locus_tag="WIE22_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ4127419.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAESEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7067..8077
                     /locus_tag="WIE22_001342"
     CDS             7067..8077
                     /locus_tag="WIE22_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ4127420.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8206..8838)
                     /gene="pdxH"
                     /locus_tag="WIE22_001343"
     CDS             complement(8206..8838)
                     /gene="pdxH"
                     /locus_tag="WIE22_001343"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ4127421.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9069..10118
                     /locus_tag="WIE22_001344"
     CDS             9069..10118
                     /locus_tag="WIE22_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ4127422.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFTHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(10187..11728)
                     /locus_tag="WIE22_001345"
     CDS             complement(10187..11728)
                     /locus_tag="WIE22_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ4127423.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQASVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         3132 a         3684 c         2990 g         2345 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacagc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cattatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct gccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaattcaa atgcaaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgccgtaaac aaatactgcc gttaatcggc tctagcgcaa acccgatgcc
      601 gtctgaagcg gcacggggtt tgtcatttct gccagtaggt tttgacgttg acgaactcgt
      661 acagcccgaa ttcggacaat tcgcgcccgt aaccggaatc tttgactccg ccgaaaggca
      721 ggcgcaaatc gctgctggta tggcggttga taaacaccga tcccgcctgt attttttcgg
      781 caaaccgcca agcgcgttcg gtatcggcgg tataaatgca ggcaccgagc ccgaacgggg
      841 aatcattggc aagggcgatg gcatgttctt cgttttcggc gcgcaaaatc agggcggccg
      901 gcccgaatac ttcttctctc cagacgcggc aggcaggatt taccctgtct aaaaccgtcg
      961 cgggataaaa ccagcctcgc ccttgtggga tttttccgcc ggtcaggcat accgcgccgt
     1021 ttgaaacggc atcttcaacc tgcccgtgaa ccctgtcccg caaatcttcg cggtgcagcg
     1081 gtgcaagcgt agtatcggga tgtttggggt cgcccatttt caatttagcg cattcggcaa
     1141 gaaacagcgt gataaaacga tcggctgcgg cttcggttac gatgatgcgc ttggcggcgt
     1201 tacacgattg ccccgcatcg cggaaacggg aataacaggc ttctgcggcg gcacgctcca
     1261 aatcagcatc gggcatcacg ataaaggcgt tgctaccgcc gagctccaac acggttttct
     1321 taaggtttgc gcccgcgtgt gccgcaagga tgcgccccgt atgcgttgaa ccggtaaacg
     1381 ccattgcatc ggtatcttca accgccttga gcgtgcccgc ctcatccagc cacacgcctg
     1441 ccagaggaat gccgtctgaa gccaaatcga acagtgcctg actgacgcgt gccacgctgg
     1501 gcgcgggttt gacggcgcac gcgttgcccg cgcacatagc gggaacggcg aaacgcaata
     1561 cctgccagac gggatagttc caaggcatga cggcaaacac cacgcccaaa ggctcgaagc
     1621 gcacctgact caaactcgcc tgcgtcgcga tggttttgtg ggcaagcagt tcgggggcaa
     1681 ggcgggcgta atagcgtatc agttcgatag acttgccgat ttccgcacgg cattcgtgca
     1741 agcagcgtcc gacttcctca cacaccattt ccgcaaaacg ctctttctcc gcctccaaac
     1801 ggtcggcaaa tttttgcagg cgcgcggcac gttcggttac gcccagttgc gcgaacgccc
     1861 cgccgcgcat tttcaaatcc gccagccgcc gttcaaactc cgcataatct tgagctggac
     1921 ggcggtaaag cgtttcgccc gtaaatacat tgacactgtg aaacatcgaa tcaacctgcc
     1981 agttgcggga atatcgtttt cagtcccgac acaataatct ccaccgatac cgccgccagc
     2041 atcataccca taatgcggtt taaaatcgtc agccccgtcg cgcccagcag gcggctgacc
     2101 ttcccggcaa cgattaaaat ggcataacaa atcgcactga ccaccaaacc ggccgcgata
     2161 atcaacgcga tgtcgccgta tgttttagcc gccgaagcgt aaataatcac ggtcgaaata
     2221 ccgcccgggc cgatggtgat cggtatggcg atgggcacga cggcaatcgc tccggcattg
     2281 cgggcggggc gcgcctgccc cgtttccggc tgcgcgccga gattctgctt ggcgggattg
     2341 tcgttgccgt tcatcataga aatggcaatc agcaacacca aaattccgcc gccgacctga
     2401 aacgaaccga cgctgatgcc caaaaccttc agcagcgtac cgccgatcag cgcaaatacc
     2461 gcaatcacgg caaacacggc aacggcggcc gtccgcgcga ccttcctgcg ctccttcgtg
     2521 ctgtgcccgt tggtcaggtc aaggtaaagc gacaacgcgc taaacggatt gatcagcacc
     2581 aaaaaagcca caatcagctt gccgatttcc atgcccaatc ccattatttc cccctccttc
     2641 aaacccgtgc ggcaggcatc cgatgctgca aattgccgcc gcaacggatt tttccgttat
     2701 aattaaaaat tcaagcaata cgccccatca tacccgaacg acggtatctt taccatcaga
     2761 caaggatgct tttcatggca ctgacacttg ccgacgtaga caaaatcgcc cgactctccc
     2821 gactgcacct gactgcggaa gaaaaagaaa aatcgcttca agaattaaac gacattttca
     2881 ctatggtcga acagatgcag aacatcaaca cagacggcat cgaaccgatg gcgcacccgc
     2941 acgaggccgc cctgcgcctg cgcgaagacg aagtaaccga aaccgaccgc gccgccgaat
     3001 atcaggcggt cgcaccggaa gtacgcgacc gcctgtacat cgtaccgcaa gttatcgaag
     3061 aataatccga atacgcttca gacggcatca gcaataccgc ccgaagccct ttaaggatgg
     3121 aagatttatg acccaataca cattgaaaca ggcaagcgtc ctgttgcagt ccaaacagat
     3181 ttccgccgtc gaactggcaa gcgcatacct tgccgccatc gccgaaaaaa atcccgccct
     3241 caacggctat atcaccatcg accaagataa aacccttgca gaagcccgtg ccgccgacga
     3301 acgtatcgcg cagggcaacg cctccgcgct taccggcgta cccgtcgcct acaaggatat
     3361 tttctgccaa accggctggc gcagcgcgtg cgcttccaaa atgctcgaca acttcatctc
     3421 cccctacacc gccaccgtcg tccaaaacct gctcgacgaa ggtatggtaa cgctcggccg
     3481 caccaatatg gatgagttcg ctatgggttc gaccaatgaa aactcattct acggtgcagc
     3541 caaaaaccca tggaatcttg agcacgtccc cggcggttcg tcaggcggtt ccgccgccgt
     3601 cgttgccgcg cgcctcgccc ctgccgcgct cggttcggac accggcggct ctatccgcca
     3661 acccgcatcg cactgcggca ttaccggcat caaacccaca tacggcacgg tttcccgctt
     3721 cggtatggtc gcctacgcct ccagcttcga tcaaaccggc ccgatggcgc aaactgccga
     3781 agactgcgcg attctgttaa acgcgatggc aggtttcgac cccaaagact ccaccagcct
     3841 cgagcgcgaa aaagaagact acacccgcga tttgaaccaa ccgctcaaag gtttgaaaat
     3901 cggcctgccc aaagaatatt tcggcgaagg caacagcgcc gatgttctga cggcattgca
     3961 aaacaccatt gatttgctga aagcccaagg cgcggaattg attgaagttt ccctgccgca
     4021 aaccaagctg tccatccccg cctactacgt cctcgcctcc gcagaagcca gcaccaacct
     4081 ttcacgttac gacggcgtac gttacggaca ccgtgccgcc caattcgccg atttggaaga
     4141 aatgtacggc aaaacccgcg ccgaaggttt cggcagcgaa gtcaaacgcc gcatcatgat
     4201 cggcacttat gtactgtcgc acggctacta cgatgcctac tatctcaaag cccaaaaact
     4261 gcgccgcctc gttgccgatg attttcagac ggcatttgca cggtgcgacc tcatcctcgc
     4321 gccgaccgca cccactgcag ccccaaaaat cggagcggat gcttcgccgg ttgaaaccta
     4381 cttgagcgat atctacacca tcgccgtcaa cctcgccgga ctgcccgcat tgaccctgcc
     4441 cgcaggcttc agcggcggcg gactgcccgt cggcgttcag cttgtcggca actacttcgc
     4501 cgaagccaaa atcctcggtg cggcgcatca aatccaactc aacagcgatt ggcacggcaa
     4561 acgacccgaa tgaagcagaa ccgcaccttt accttccccg attttcgcac cgtttacagc
     4621 tatgcgcctt tatatcggct gcaacattta aaatacacat tgcgaaaatt tttcggaaaa
     4681 aaagaaattt acgccttcga gcagtttgtc aacgcctccc ctatccgtca ggggctgttc
     4741 ctccactgcc cgcaaaatgc ctatccgctg ctgcgcgaat ttgttgacag gcgttttaac
     4801 tgcaaacgcc gtttagatgc gatgacggca gattttctca tggcggaaaa actgttcggc
     4861 acagacatcc tgcaccaaat ggaagactac cgcttccatt tggtcttggc gcacctttca
     4921 gacggcatca gcttgtggct caaccgcaac gacaactgcg tcgaagaagg cgcgtggtct
     4981 ttatctttgc gcgacgaagc aggcaaccgg ctgtatatgg cgactttcgc ctttgtcggc
     5041 acacacctgc tgacagcctc cgtacaaggg cctgcgggtg aagaagccaa agacactgtc
     5101 cgccgcataa ccaaacaact tcacggcttg cgtccccaac aactgatggt aaccgccctg
     5161 caatatttcg ccgccgtact cggcttggac ggcgcaatgg gcattgcaca aaaacatcag
     5221 gtcaaactgc gctggaaact taaaaagcgt gtcaaaatga attacgacgc attctggcag
     5281 gaatacggcg caagtttgga acgggacggc tactggcatc tcccccaaac ccccgcccgc
     5341 aaagacctta ccgacatcga aagcaaaaaa cgctcgatgt accgcaagcg ttatgaaatg
     5401 ctggacaaca tggttgcaga gatgaaggac ggtctgaaaa cagaagcacg cggcatttca
     5461 gacggcatcc aaacggaaaa accgctccgc cggacagcct gacgcgaaga ctatcgaatt
     5521 gatattttag agaaagaagc tcttatgacc tgggaaaccg taatcggctt ggaaatccac
     5581 gtccaattga acaccaaatc caaaatcttc agcggcgcat cgaccgcatt cggcgcagaa
     5641 cccaacgcgc acgccggcgt agtggaatgt gctttgccgg gcgtactgcc ggtaatgaac
     5701 cgagaagtcg ttgaaaaagc catcaaatta ggcttggctt tggatgcgaa aatcaaccgg
     5761 aaaaacgtgt tcgaccgtaa aaactacttc tatcccgact taccaaaagg ctatcaaatc
     5821 agccagttgg acttaccgat tgtcgaacac ggcaaattgg aaatcgtagt cggcgacgat
     5881 gtgaaaacca tcaacgtaac ccgcgcccat atggaagaag acgcgggcaa gtccgtacat
     5941 gaaggcttga acggcgcgac cggtatcgat ttgaaccgcg ccggcacgcc tttattggaa
     6001 gtcgtatccg aacccgaaat gcgctccgcc gccgaagccg ttgcctacgc caaagccttg
     6061 cacagcttgg taacctggct gaacatttgc gacggcaata tggcggaagg ttcgttccgc
     6121 gtcgatgcca acgtatccgt tcgcccgaaa ggtcaagcgg aattcggcac gcgccgcgag
     6181 attaaaaacc tcaattcctt ccgtttcttg gagcaggcga ttaattacga agcagaagcg
     6241 caaatcgaga ttttggaaga cggcggcaaa gtacagcagg caacgatgct gtttgatccc
     6301 gaaaaaggcg aaacccgcgt aatgcgcctg aaagaagacg cgcaagacta ccgctacttc
     6361 cccgaccccg atttgctgcc cgtcatcatt tcagatgccc aaatgcaaaa agccaaagca
     6421 gaaatgcccg agctgccgaa agaaatggca gcgcgtttcg tggcggacta cggcgtgtcc
     6481 gaatacgacg cgcgcctgct caccgcaagc cgcgcacagg ccgcctattt tgaagaagcc
     6541 gccaaagaga gcggacaagg caagccgact gccaactgga tgaacggcga actcgccgcc
     6601 gcactgaaca aagaaggcat ggaacttgcc gacagcccga ttaccgctcc gcgcctcgcc
     6661 gcgctggtag gcaaaatcgc cgacggcaca ttaagcagca aattagcgaa aaaagccttt
     6721 gaagccatgt gggcagaatc cgaagccacc attgccgaaa tcattgaaaa acacggtttg
     6781 cagcaaatga ccgataccgg cgcgattgaa gccatggtgg acgaagtgct ggcaaacaac
     6841 gccaaagccg tggaacagtt caaatccggc aacgaaaaag ccctgaatgc gattgtggga
     6901 caagtgatga aggccagcaa aggcaaagcc aaccccgcgc aggttcaaga gctgattaaa
     6961 gccaaacttg cctgacaagc aagatgccgt ctgaaaccca aatccgacgt tcagacggca
     7021 ttttcacagt ctgaccacat taaaacagca taaaaaacaa tattccatga accacaccgt
     7081 taccctgccc gaccaaacca cctttgccgc caacgacggc gaaaccgttt tggccgctgc
     7141 cgcccgacaa aacctcaacc tgccccattc ctgcaaaagc ggtgtctgcg gacaatgcaa
     7201 agccgaactg gtcagcggcg atattcaaat ggacggacac tcggaacagg ctttatccga
     7261 agcagaaaaa gcgcaaggca agattttgat gtgccgcacc accgcgcaaa gcgatatcag
     7321 catcaacatc cccggctaca aagccgatgc cctacccgtc cgcaccctgc ccgcacgcat
     7381 cgaaagtatt attttcaaac acgatgtcgc cctcctgaaa cttgccctgc ccaaagcccc
     7441 gccgtttgcc ttctacgccg ggcaatacat tgatttactg ctgccgggca acgtcagccg
     7501 cagctactcc atcgccaatc cgcccgacca agaaggcatt ttggaactac acatccgcag
     7561 gcgcaaaaac ggtgtctgct cggaaatgat tttcggcagc gaacccaaag tcaaagaaaa
     7621 aggcatcgtc cgcgttaaag gtccgctcgg ttcgtttacc ctgcaggaag atagcggcaa
     7681 acccgtcatc ctgctggcaa ccggcacagg ctacgccccc atccgcagca tcctgctcga
     7741 ccttatccgc caaaacagca gccgcgccgt ccatttctac tggggcgcgc gtcatcaaga
     7801 tgatttgtat gccctcgaag aagcacaagg gttggcatgc cgtctgaaaa acgcctgctt
     7861 cacccccgta ttgtcccgcc ccggagaggg ctggcaggga agaaaagggc acgtacaaga
     7921 catcgcggca caagaccacc ccgacctgtc ggaatacgaa gtatttgcct gcggctctcc
     7981 ggccatgacc gaacaagcaa aaaatctgtt tgtgcaacag cataagctgc cggaaaactt
     8041 gtttttctcc gacgcattca cgccgtccgc atcataattc cccggtataa aaaagattca
     8101 ggctttccgt tcggaacaca aaaaacttcc cgtccgtatt ttccccataa aaaatgccgt
     8161 ctgaagcccg attccggttt tcagacggca tatgtttttt cctgttcaag gcgacagccg
     8221 ctcgcgtatc cagccaccat ccagcaaacg gtattggatg cggtcgtgca gcctgctcgg
     8281 cctgccctgc caaaactcaa tcaaatcggg aatcacaata tagccgcccc aatgcggcgg
     8341 acgcggcacg tgcaaaggat gtttgagtcc gaccgccgcc gcctttgcca ccaataccgc
     8401 cttgttcgga atcacctcgc tctgcgcact tgcccacgca cccaaacggc tctgatacgg
     8461 gcggctctca aaatattcgt ccgacaactt ctccgccagc ctttcaacac gcccttccac
     8521 gcgcacctga cgctccagct ccggccaaaa aaacgtcatc gccgcaaatg gatgagcatc
     8581 cagcgaacgt cccttacggc tgtgataatt cgtaaagaaa acaaaacctt cagaattaac
     8641 ttccttcagc agcaccatac ggctgttggg tctgccgcgt ccgtcgaccg ccgccacatt
     8701 aaccgccgtc ggctcgttga cctgtgcgcg taccgcctcg tccaaccacc gctcgaactg
     8761 ctcgatcgga ttgtcggcgc aatcgccttc cgacaattcc cgtttactgt aatcttcccg
     8821 aatattgtgc aaatccattt accgctcctc ttgtcatttg aaagattcta cccccgcacg
     8881 caaaccgatt tcaaccgttg cacaaacttt gcctcgcttc tccaaacctc aggacacaac
     8941 gatttcatcc gcaaaaccgc cgcataaggt acaatatcga accgtccgac cgagggcggc
     9001 attttatcaa cccgtcctgc cgcacacgcc gcagaagaac cgccttatca ggcgagttag
     9061 gaaaaatgat gtccaaacag cccaccagca aacgccaatg gcgcgacggc gtagccccgt
     9121 ctgccaagaa aaccgccaaa cctttcaaaa gcaaagcccg tcccaaagac gaaacggaca
     9181 aaaccgcatc ccaaccttac ggacaaaaag cttcagacgg catcaaacct caaaacctcc
     9241 ccaaacagcg cgccgccaaa gccaaaaaac tcgtcgtccg caatcccaac caaaaaatta
     9301 tggaacacgc gcgcgattta aaagaacgcc gcagcgacct atcgcgcatg gaacccgaac
     9361 gcctgcaaaa agtgcttgcc gcgtccggcg tcggctcgcg ccgcgaaatg gaagaatgga
     9421 tcaacaacgg ctgggtaacg gtcaacggca aaaccgcgca actgggcgac aaagtcaccc
     9481 ccgacgacca cgttaccgtc aaaggcagca tcatcaaact caaatgggcc gaccgcctgc
     9541 cgcgcatcat cctatattac aaacaagaag gcgaaatcgt ttcccgcgac gacccgcaag
     9601 gccgcgtcag catattcgac cgcctgccgc aggccgccag cagccgctgg gtcgccatcg
     9661 gacgcttgga catcaacacc agcggacttc tgattctcac cacctccggc gaactcgtcc
     9721 aacgtttcac ccaccccagc ttcgaagtcg aacgcgaata cgccgtgcgc gtactgggcg
     9781 ggctgaccgg cgaacaaatg cgcgtcctca ccgaagaagg cgtgatgctc gaagacggct
     9841 tggcaaaagt cgaacgcatc cgcgaacaag gcggcgaagg cgcgaacaaa tggtacaacg
     9901 tcgtgattaa agaaggccgc aaccgcgaag tgcgccgcat ttttgaaagc caaggactca
     9961 ccgtcagccg cctcgtgcgc gtcggcttcg gccccatcgg actgcccaac cgcctcaaac
    10021 gcggacagtt ctacgaactc aaccccgccg aagtcgccaa catcctcaaa tgggcggaaa
    10081 tgctgctgcc gggcgaacgc cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc
    10141 cgtctgaaac atctactgtt tcagacggca ttttattcgg gcgttttcag gagaaaaggt
    10201 cgagtgcttt gacaaagacc atcaccacgc cgtaggcgag cggtatgacg gccaatgccc
    10261 aacgccacca gaccgatatg ccgccgccgg aaactttttc gcccacaagg taagcgtcgg
    10321 atatggcggt ttcgtcatcg gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt
    10381 ggtgtttttc gtgtacggat ttgacggcga ggttgcacaa caaaccgata atcagcaggc
    10441 acgccatgat gtacatggtt acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt
    10501 ggctttggcg tatgtaattg accagtaccg ggccgatgac ggcggcggtt gaccaggcca
    10561 gcaggatgcg cccgtgaatc gcgccgacct gataggtgcc gaacaggtct ttcaggtagg
    10621 cgggaatggc ggcaaatccg ccgccgtaca tggaaataat cacgcaaaag ccgatgatga
    10681 acagggcttt gctgccgccc tcgccgatgg agggaacggc gaaatacagc agcgaaccga
    10741 gtacgaagaa aatggtgtag gtgtttttgc gtccgatttt gtcggaaacg ctcgaccata
    10801 aaaagcgtcc gcccatgtta aacaggctca ggaggctgac gaagcctgcc gccgcacctg
    10861 cgccgactga cgcctgcctg cctacggagg tttcggaaaa gagttcctga atcatcacgg
    10921 atgcctgacc caatacgcca atgccggcgg ttacgttcag gcacaatacc cagaacaaca
    10981 gccaaaattg cggcgttttc atggcttgtg acacgttgac atgattgctg ctgaccagct
    11041 tgttttgcgt tttcggcgcg gtatagcctt caggtttcca gccgtcggca ggtacgcgga
    11101 tggtaaacgc gccgaacatc atcagtgcga ggtaaagcag gcccaatacg gcgaaggttt
    11161 caacaacccc gactgaagcg gcgtttgaaa aggcgttcat cagtgatacg gaaagcggag
    11221 aggccagcat tgcgccgccg ccgaaaccca taatcgccaa accggtcgcc atacccggct
    11281 tgtcgggaaa ccatttcatc agtgtggaaa ccggcccgat gtagcctaag cctaagccta
    11341 cgccaccgat gacgccgttg cccaaataaa gcaggaagag gttgtgcgta ctcacgccga
    11401 atgcggatac gaagaagccc aggctgaaac agcaggcggc ggcaaatatg gctttgcgcg
    11461 gccctacccg ttccatccat gtgccgaaca gggcggccga cgcgcccagc atcgcgagtg
    11521 cgatactgaa aatccaacct acggtcgtca gcttccaatc tccggccgcc gattcggtta
    11581 tgccgataag tttggtcagc ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg
    11641 caaggtgtac cgccaatgct gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa
    11701 tgcttgcctc acggtctaaa aacttcataa catcctcttt ctgtcagttg aaaaataaaa
    11761 tttcatttgc tcaatggaaa cttattgaaa actataaaaa aatatcggat cggtttttta
    11821 tccgccccaa gatgccccgt ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc
    11881 cgacaaattc ctgcggcttt tcgcttccgg attcccgctt ttgcgggaat gacgaattaa
    11941 agattatctt aaggtcaagg gactggattc ccgcttttgc gggaatgacg gcggcgggtt
    12001 gctgtttttt atttataaat ttctgtaact ttggatttca gccatcttca ataaattatc
    12061 gaaacgtatc aatcgtcatt cccgcgcagg cgggaatcca tccagttcgt tcgatttcgg
    12121 ttttttaagt ttcgagtaac ttctgactcg t
//
LOCUS       DAWHXS010000063        11814 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWHXS010000063 DAWHXS010000000
VERSION     DAWHXS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11814
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.063"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            529..1830
                     /gene="bioA"
                     /locus_tag="WIE22_001346"
     CDS             529..1830
                     /gene="bioA"
                     /locus_tag="WIE22_001346"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ4127424.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1827..2474
                     /gene="bioD"
                     /locus_tag="WIE22_001347"
     CDS             1827..2474
                     /gene="bioD"
                     /locus_tag="WIE22_001347"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ4127425.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2489..2671
                     /locus_tag="WIE22_001348"
     CDS             2489..2671
                     /locus_tag="WIE22_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127426.1"
                     /translation="MEHLYDEYGNTTPNRRTTNKKDETISSKVTVAAIPVAAPFAIAD
                     IVDQDFIQAIMKITGH"
     gene            2695..3081
                     /locus_tag="WIE22_001349"
     CDS             2695..3081
                     /locus_tag="WIE22_001349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127427.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3075..3518
                     /locus_tag="WIE22_001350"
     CDS             3075..3518
                     /locus_tag="WIE22_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127428.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            3700..3972
                     /locus_tag="WIE22_001351"
     CDS             3700..3972
                     /locus_tag="WIE22_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127429.1"
                     /translation="MTIRANNDIEYFVKNNTTNEQRIDDFRSCLSVACPEIYKLYQLP
                     DEKGNYQYIIKKYYDNKYNDNAVKSTNCIGDNARCMLNNKGYSTKW"
     gene            3975..4451
                     /locus_tag="WIE22_001352"
     CDS             3975..4451
                     /locus_tag="WIE22_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ4127430.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4481..5371
                     /gene="ubiA"
                     /locus_tag="WIE22_001353"
     CDS             4481..5371
                     /gene="ubiA"
                     /locus_tag="WIE22_001353"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ4127431.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5556..6005
                     /gene="ptsN"
                     /locus_tag="WIE22_001354"
     CDS             5556..6005
                     /gene="ptsN"
                     /locus_tag="WIE22_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ4127432.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            6007..6969
                     /locus_tag="WIE22_001355"
     CDS             6007..6969
                     /locus_tag="WIE22_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ4127433.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            6950..7804
                     /gene="rapZ"
                     /locus_tag="WIE22_001356"
     CDS             6950..7804
                     /gene="rapZ"
                     /locus_tag="WIE22_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ4127434.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            7885..8826
                     /gene="ylqF"
                     /locus_tag="WIE22_001357"
     CDS             7885..8826
                     /gene="ylqF"
                     /locus_tag="WIE22_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ4127435.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGKKPSEA"
     gene            8927..9529
                     /locus_tag="WIE22_001358"
     CDS             8927..9529
                     /locus_tag="WIE22_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ4127436.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            9713..11386
                     /gene="recN"
                     /locus_tag="WIE22_001359"
     CDS             9713..11386
                     /gene="recN"
                     /locus_tag="WIE22_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ4127437.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            11496..11572
                     /locus_tag="WIE22_001360"
     tRNA            11496..11572
                     /locus_tag="WIE22_001360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11530..11532,aa:Arg,seq:acg)
     gene            11590..11664
                     /locus_tag="WIE22_001361"
     tRNA            11590..11664
                     /locus_tag="WIE22_001361"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11623..11625,aa:Glu,seq:ttc)
BASE COUNT         3044 a         2785 c         3049 g         2936 t
ORIGIN      
        1 cctgattttt gttaatccac tataaaatag ggctgtaacc ttcaatcgga atttgttgcc
       61 tgcgggatat gcggtatgaa tgtttgggat atatggggca ggatggtgga aatctatcat
      121 aagtataaga agccgtgcct ggttttggcg gtggattttg tgatgggtat ggtattcata
      181 gagccgaatg aggagctgtg catcggtaga tgctatgcgc ctatgtcgga gtcccctgat
      241 tttgctaacg ctgttgcgat ggctgttgct atgatctgta tcgtatggat tgccgttttg
      301 tctatgactg attgaagcgt tgggcggagg ctgtgtgaaa caatattggg cgttgggtcg
      361 tctgattcca atcgggcttg gggaatgcga aacggtgtgc gcttatactg cggacgattt
      421 gttgcgtggt tttacgcccg aaacggatgg aaaggtgtgg gaagcggtct gtcggagtag
      481 aatacgcgtt ttgcgtttga atgcagcaat aagaaaagag agaaacttat gccgtctgaa
      541 catcaacaca catcatcatt gcttgatttc gaccgtaccc atctgctcca tccctatact
      601 tccatgaccg atccgcttcc cgtttatcct gtcaaacgtg cagaaggggt gtttatcgaa
      661 ttggcggacg gcacgcggct gattgacggg atgtcgtctt ggtggtgtgc gatacacggt
      721 tacaatcatc ccgttttgaa tcaggcggtt gagacgcaga tgaaacagat gtcgcatgtg
      781 atgttcggcg gtttgacgca tgagccggcg gtggagctgg gcaaattgtt ggtcgggatt
      841 ttgccgcagg ggctggatcg tattttttat gcggattcgg gttcggtttc ggtggaagtc
      901 gcgttgaaga tggcggtgca ataccagcag gcgcggggtt tgcgtgcaaa acaaaatatg
      961 gcgacggtgc ggcgcgggta tcacggcgat acttggaacg cgatgtccgt ctgcgatccg
     1021 gaaacgggga tgcaccatat tttcggcagc gcgttgccgc agcgttattt tgtcgataac
     1081 ctgacaagcc gtttcgacga tgaatgggac gaggcggatt tgcagcctgt ccgcgccttg
     1141 tttgaagcgc atcatacgga cattgccgcc tttattttgg aaccggtcgt gcagggcgcg
     1201 ggcggtatgt atttttatca tccgcagtat cttcgcggat tgcgcgattt gtgcgacgag
     1261 tttgatatcg ttttgatttt tgatgaaatc gccaccggat tcgggcgcac gggcaagatg
     1321 tttgcctgcg aacacgcgga ggtcgtgccg gatattatgt gcatcggcaa gggtttgagc
     1381 ggcggctata tgacgctggc ggcagcaatc acttcgcaaa aagttaccga aacgatttcg
     1441 cgcggagaag cgggcgtgtt catgcacggc ccgacattta tggcgaaccc gctggcgtgt
     1501 gccgttgcct gcgcttcggt caaactgctt ttgtcccaag attggcaggc aaatatccgc
     1561 cgcatcgaaa gcatcttaaa atgccgtctg aaaaccgcgt gggacattcg tggtgtgaaa
     1621 gacgtgcgcg ttttgggcgc catcggcgtg atcgagttgg aaaaaggcgt ggatatggcg
     1681 cgttttcaag cggattgcgt ggcgcagggc atttgggtgc gcccgttcgg caggttggtg
     1741 tatctgatgc cgccttacat catttcagac ggcgttttga ccaaacttgc cgacaaaacc
     1801 gtgcaaatct tgaaggaaca cagcaaatga aaggcgttta cttcgtcagc ggcatagaca
     1861 cggacatcgg caaaaccgtc gccaccggcg tgttggcaaa acaattgttg cagcagggca
     1921 aaagcgtgat tacgcaaaag cccgtgcaaa ccggttgcca aaacattgcc gacgacatcg
     1981 ccgtccatcg taaaatcatg ggtaaaccca tgcaggaagc cgacaaacaa gggctgacca
     2041 tgcctgaaat cttcagttat ccagcttcgc ctcatctcgc tgcccgtctt gacggcaggg
     2101 ctttggactt ggacaaaatc cgcaccgcca cacaagaatt ggcggcgcag tacgaagtcg
     2161 ttttggtcga aggcgcgggc ggattgatgg ttccgctgac ggaaaacctg ttaaccattg
     2221 attatatccg tcagcaaggc tatcccgtca tcctcgttac cagcggacgg cttggcagta
     2281 tcaaccacac tttactcagt ttcgcagcgc tcaaacaata cggcattcgc ttgtacagcc
     2341 tcgtgttcaa ccacatccac gacagccgcg acgcacacat cgcccaagac agcctgagct
     2401 acttgaaatg ccgtctgaaa gccgattttt ccgaagcgga atggatggag ttggcaaaaa
     2461 cagacgcggt ataaagattg ggaaaaatat ggaacaccta tatgatgaat atggaaatac
     2521 aacgccaaat agaagaacaa caaataaaaa agatgaaact atttcaagta aagtaactgt
     2581 tgcagcgata ccagtagccg ctccatttgc tattgctgat atagtcgatc aggattttat
     2641 ccaagcgatt atgaaaatta ctggtcattg attatctata tattggtggt tgttatgaaa
     2701 tgctatatta aagttgtcat tattgttttt ataatggtaa tgacaacttc ttgtaatcct
     2761 tgcttttttg caacttcttc ttgtgattat tcttttagaa aaaactatcc tataaatagc
     2821 gacgatgaat attatgttaa gaataatacg agcaaagaaa caagaattgc ggatttacgc
     2881 tattgtattt cttatgcatg tccagaagtt tatcaaaaat atcaactacc agatgaaaat
     2941 ggagattata gggagattat tcgtagaaaa tataaagatg attataaaaa gggctataag
     3001 aatgcagatg atgtcatgac atgctacgga aagattggag aatgcatgaa aaataataaa
     3061 ggatattcaa caaaatggta atgttccgct tattatcaac agcccttgta ttgttgataa
     3121 tgcagggctg ttattacaca actattgata atgaaaaatg ctttgttgcc ccacaaagcg
     3181 ttcattgtcc tgtaagttct gatattgaaa atgttattag aaaaaatcct aataaaagca
     3241 taaatcaagt taggagtgat attaaatatt gtgctaatat gtatttttcc aatatacggg
     3301 aattaaatat tccgcaagat tttgataatt acattttcat gcttaaagaa aaaggtattc
     3361 caagaaatga taaggaattg gcttataatg cagttagaaa tatgcacatt atttgtttga
     3421 gttctttggg tggttatgat tattatcaaa aaaccgcaga atttcagaaa atagattata
     3481 aaaaagaaat ggagaaattg ttaaatgaaa accattaaat ttgctattgc cgatatagtt
     3541 gatagcgact ttatccaagt gattatgaaa ttaagcgggc attgatttat gattacgcgt
     3601 tgttttaaag ttgtcattat tgtttttata atggtaatga caacttcttg taatccttgc
     3661 ttttttgcaa cttcgtcttg taattatcct tttgatgata tgacgataag agcaaacaac
     3721 gatattgaat attttgttaa aaataatacg actaacgaac aacgaattga tgatttcaga
     3781 agttgccttt ccgttgcttg tcctgaaata tataaattat atcaattacc tgatgaaaag
     3841 ggtaattatc aatatattat aaaaaaatat tatgataata aatacaatga taatgcagtc
     3901 aaatcgacaa attgtattgg ggataatgca agatgtatgc ttaataataa aggatattca
     3961 acaaaatggt aataatgggt tacggctttg aggaatggct gcccgacctg cccgcccctg
     4021 tttcagacgg catcggcctg ccgatgagcc ggttattgaa agcccggtcg ctgaccgccg
     4081 cattgcgcgc cttgccgcat acattttcgg tggaactgct gaaactgggc gaattggaga
     4141 cggaatgcgg agggaggctg gtgcgcgaag ttttgttgaa gctggacggt atccctgttg
     4201 tcgcggcaag gagcgagtgc cgtatcggtt cggcgttttg gcaaaacatt ttggactgcg
     4261 gcacgcgtcc tttgggcgag cgtctgtttc aagccgattt ggaaggggcg cgttcggcgt
     4321 ttgagtttgc cgtttccggc gaaggatgcg gacggtactt tgccgcgcgg cgttctcggt
     4381 tttcccgtca cggcgaggaa atgctgctga ccgagtattt tctgcccgaa ctgaaacgct
     4441 ttatcggata aaataccgtt ttttcaagct gtgcggcaat atgaatccta aatccccttt
     4501 atttttacga ctgtccgatc gtttggatgt gtacctgcgc ctgatgcggg cggacaagcc
     4561 cattgggacg ctgcttttgc tgtggccgac ctactgggca ttgtggctgg cttcagacgg
     4621 cattcccgat ttggcggtat tggcggcgtt tacaatcggc acgtttttaa tgcggagtgc
     4681 cggttgcgtc atcaacgatt ttgccgaccg cgattttgac ggtgctgtcg agcgcaccaa
     4741 aaaccgtccg ttcgcacagg gtcgggtcgg aaaaaaagaa gcgctgctgc tgacggcatt
     4801 tttgtgcctg cttgccgcat tgtgcctgat tccgctgaat catctgacat ggctgatgag
     4861 cctgcccgcg ctgtttcttg cgctgactta cccgtttacc aaacgctttt ttccgattcc
     4921 ccaactctat ctcgggcttg ccttttcctt cggtatcccg atggcgtttg ccgccgttgc
     4981 cggaaacgtg ccgcctcaag cgtggatact ctttgccgcc aatgtgttat ggactctggc
     5041 gtatgacacg gtttatgcaa tggcggacaa agaagacgat ttgaaaatcg gcatcaaaac
     5101 ctccgccgtc acgttcgggc gttacgacat cgccgccgtt atgctgtgtc atggcggctt
     5161 taccctgctg atggcagtat tgggtgcggt tatcggtgcg gcatgggcat attggacggc
     5221 aatccccatc gtcctgctgc tgcaataccg ccaatatgcc gccatcaaaa gccgcgtccg
     5281 gcaaatctgt tttgaaacgt ttttggcaaa caacagaatt ggttgggtgt ggtttaccgc
     5341 catttttgcc catacgtttt tcgcgaaata aggcagggca atgccgtctg aagagccgta
     5401 aactgctttg gacggcattt ctatctgtgc cgaaaagcgt taaaatatgt ttttaaaacg
     5461 ctgtgttatg tcagcccgta ccgtatgcgg gattgagatt tgccctagca gtcggtacaa
     5521 tctttctgtt ttgcgatgtc tgaaaagaga agcttatgag ccttatcggc gaaattttgc
     5581 ctttgtccca tattgttttg gatatggagg tgggcagtaa aaaaaggctg tttgaggaag
     5641 cgggcctgct tttggaacgc gaatcctcat tgtcccatgc tgatgttttc gaatgtcttt
     5701 ttgcccgtga aaaactcggt tcgaccggtt tggggcaggg cgttgccatc ccgcacggcc
     5761 gtcatgccgg cgtgaagcag gcgacgggcg cgttcatccg cacgcgcgaa cccgtcggat
     5821 ttgacgcacc ggacggcaag ccggtttccc tgatttttat cttgctggtg ccggaaaacg
     5881 caactggcga acatttggaa gtcttatcca aactggccgg caagttttcc caaaaaagca
     5941 tccgcgaatc gctgatgacg gtttcttctg cggaagaagc ccgcgccatt ttgactgaag
     6001 aataatatgc ccagtatttc cgtccgccga ctgtttgacg acaaccaata caagttacaa
     6061 cttgcctggg ccgccggcaa ttcgggtgcg gacaaccgta tcggcgtaga ggcggacaag
     6121 cccgtcctcg ctttggtcgg acacctgaat ttcatccacc ccaaccaaat ccaagtggtc
     6181 ggtttggcgg aatcggaata tctgaaccgt ctcgaatcgg gggaaacggg ttatcagttc
     6241 ggcgacctgt tcgacatttc tatgtctttg gttattgtgg caaacggctt gccggtttcc
     6301 ccggggctgc gcgactattg ccataaaaac gatattccac tgctgacttc caaactcgaa
     6361 agcccctatc tgatggacgt gttgcggatt tacctgcaac gcaccctggc ggcatcgtcc
     6421 gtcaaacacg gcgtatttct cgatgtgttt gaaatcggcg tgctgattac cggacattcc
     6481 ggcttgggca agagcgaatt ggcgttggaa ctgatttcgc gcggccacag cctgattgcc
     6541 gacgatgcgg tcgagctgtt ccgcatcggt ccggaaacat tggaagggcg ttgttcgcct
     6601 atgctgcgtg attttttgga agtgcgcggc ttggggatac tcaatatccg ccatattttc
     6661 ggcgaaactt ccatccgccc caaaaaaatc ctgcaactca ttatcaattt ggtcgaggca
     6721 gacgacgagt atatgaagca gctcgaccgg ttgagtatcc gcaccgaaac cgaatccatc
     6781 ctcaacgtca acgtccgttc ggttacgctg cccgtcgccg tcggacgcaa cctcgccgtt
     6841 ttggttgagg cggcggtacg caattacatt ttgcagttgc gcggtaagga cagtacgcgc
     6901 gaatttttgg aacgccatca gacgcaactt aaagaaaacg aacagaatca tgaaaatcgt
     6961 cctgattagc gggttgtccg gttcgggcaa atccgtcgca ctgcgccaaa tggaagattc
     7021 gggttatttc tgcgtggaca acctgccctt ggaaatgttg cccgcgctgg tgtcgtatca
     7081 tatcgagcgt gcggacgaaa ccgaattggc ggtcagcgtc gatgtacgtt ccggcattga
     7141 catcggacag gcgcgggaac agattgcctc tctgcgcgga ctggggcaca gggttgaagt
     7201 tttgtttgtc gaggcggaag aaagcgtgtt ggtccgccgg ttttccgaaa ccaggcgagg
     7261 acatcctctg agcaatcagg atatgaccct gttggaaagc ttaaagaaag aacgggaatg
     7321 gctgttcccg cttaaagaaa tcgcctattg tatcgacact tccaagatga atgcccaaca
     7381 gctccgccat gcagtccggc agtggctgaa ggtcgaacgt accgggctgc tggtgatttt
     7441 ggagtccttc gggttcaaat acggtgtgcc gaacaacgcg gattttatgt tcgatatgcg
     7501 cagtctgccc aacccgtatt acgatcccga gttgaggcct tacaccggta tggacaagcc
     7561 cgtttgggat tatttggacg gacagccgct tgtgcaggaa atggttgacg acatcgaaag
     7621 gtttgttacg cattggttac cgcgtttgga ggatgaaagc aggagctacg ttaccgtcgc
     7681 catcggttgc acgggaggac agcaccgttc ggtctatatt gtcgaaaaac tcgcccgaag
     7741 gttgaaaggg cgttatgaat tgctgatacg gcacagacag gcgcaaaacc tgccgagccg
     7801 ctaatcccgt tgaacgatga tgccgtctga aacccgattt tctgtttcag acggcatttc
     7861 aaatattgag aagaaaggaa agacatggca atccaatggt ttcccggcca tatgaacaaa
     7921 gcgaaaaaag ccatcgccga gcgcgccaaa agcgtagaca tggtgattga aatgctggat
     7981 gcgcgaatgc ccgcctccag cgaaaacccc ctgcttgccc agctttccaa aggcaaaccc
     8041 aaacttaaaa tcctaaacaa acaagatctt gccgaccccg aacgcacaaa ggtctggctt
     8101 gaccactaca acagccgccc cgacacgtgc gccattgccc tcgattcctc cgaaaccggc
     8161 gcacacggca aaatcaccca agcctgccgc gcgatgattc cccatcggca aggcatagac
     8221 aaacccttgc gcgtcttgat ttgcggcatt cccaacgtcg gcaaatccac cctcatcaac
     8281 ggcatgatag gcaaaaaatc cgccaaaacc ggcaacgaac ccggcatcac caaagccgaa
     8341 caacgccttt tccttgccga cgacttctgg ctgtacgaca cccccggaat gctgtggccg
     8401 aaaatcattg tcgaagaaag cggctacaac ctcgccgccg gcggcgcagt cggacgcaac
     8461 gcattggacg aagaagaagt cgccctcgaa cttttagact acctccgccg ccactacctc
     8521 cctatgttgc aagaacgcta ccaagccgac aaagacccca gcagccactg ggacgaaaac
     8581 gtttggctcg aatggatagc caaaaaacgc ggcgcagtcc tcagcggcgg acggatcaac
     8641 taccaaaaag ccgccgaaaa catcctcacc gacttccgtg aaggcaaaat cggcagaatc
     8701 accctcgaaa cgccgaacca atgggaaact tggctcaaaa aagcccgtca gaaagaagcc
     8761 gaactcaaag ccatacgcga agccagaaaa gcagagcgta aagggaagaa gccttcggaa
     8821 gcataaagaa tgccgtctga aaaatatttt tcaggcagct tctctctact ccaaccgatt
     8881 tcagacggca tatccaaacc catgccgttt cagcacggat acccgtatga ccgacaaaat
     8941 ttctcccgac gcgctgattg aagccgcact gctgacccaa accgaaccgc tgaccgaaaa
     9001 atctatgcgc gaattgtgtg tgccgccact gtcgcaggac aaactgattg atgtgttggc
     9061 gcagttgaaa acgcgttggc aggatagggc gttgcaactg gtgcatacgc aagagggctg
     9121 gcgttttcag attgttcaga cggcattcga gcggctgggc agcctgcaag aacagcgtgc
     9181 gccgcgctac tcccgcgccg tgatggaaac actggcgatt atcgcctacc agcagcccgt
     9241 aacgcgcggc gacatcgaag gcatacgcgg tgtggcggtg tcgcagaacg tgatgcagac
     9301 cttgcaggat cgggggtgga ttgaagtcat cggacatcgg gacacattgg gaaaacccgc
     9361 attgtgggcg acaacggcaa cttttctcag cgatttgcgc ttggacggtc tggaagaact
     9421 tccgccgctg accgaactgg gcgaactggt tttacccgat ttaatggaaa tgccgcctac
     9481 ggatgaagaa gagccggaag ccgtaccgtc cgataccctg cccaactgaa attccaaatg
     9541 ccgtctgaaa cgcacattgc ttcagacggc atttcaacaa ataagcagat aaaaacaagc
     9601 actaagcaaa attaaggaaa aacttatttt taatttaaaa aatcttagtt ataattcgta
     9661 tatctaaagt tgatattgct tttgtcggta gaattgctaa ggaatcctca cgatgcttct
     9721 aacactttct ttgcgtgatt ttgtcattgt cgaaaatctg aatctggatt ttcaaagcgg
     9781 ctttaccgta ttgaccggag aaactggcgc gggcaagtcc attactttgg atgcgattgg
     9841 tctgctgttg ggcgataaag ccgattacag ccaagtccgc agcggcgcaa aagaagcgca
     9901 gttgtcggcg ttgtttgata tttcccattt acctgtttta aaagcagaat tgtatgaaca
     9961 ggggctttta aacgacggag aagaagaact cagtatccgc cgcattatcg atgccaaagg
    10021 caaaagccgc agctttatca acaatcaggc cgctaccttg gcgcaactca aagccgtcgg
    10081 cagccagctt atcgacatcc atgggcaaaa cgcccatcat tcgcttaatc aggaagccgc
    10141 ccagcgcgaa ttgttggacg catttgcggg cagcagggcg caggcggaaa ccgtcaggca
    10201 gctttatcaa aactgggcaa actcaaaaaa agcattgcag gaagcgcagg aacaggcaga
    10261 aaacattgca ctcgaacaag agcgtctgga atggcagttt aacgaattga atcagttgga
    10321 cattaaacaa ggcgaatggg aagccctcag ccaaagccac gacagccttg cccattctgc
    10381 cgagctgttg caggctgccg aagaagtcgg aagcaagatt gacggcgaca acggcatcca
    10441 acgccatatc tatcaatgtc aaaaactatt ggccaatctg caaaacatcg agccgcgctt
    10501 tgccgagagc ctgaatatgt tggcaagcat cgaagccgaa ttgggcgaaa tcagtgccaa
    10561 tatgcgcgat gtggcaggtc gcagcgacat caatcccaac gaacttgccg cacaagagca
    10621 gcgtatgggc gagctgatgg ggatggcgcg gaaataccgg atcgagcctg aagagttgcc
    10681 tgccaagttg gcagaaatcg aagaacgcct gcaaagcctg caagctgccg ccgatttgga
    10741 cgcgctcgag cataatgttg cccacaattt tgccgaatat caggaagctg cccacatcct
    10801 ttctgccatg cgccatcagg cggcagagcg tttgagcggc gaaacgaccg agcatatgca
    10861 acaccttgcc atgaaaggtg cacgtttcga catcgtcctg ttgccctcgt cgccgacggc
    10921 acacggtttg gagcaggttc aatttcaagt tgccgccaac aaaggcaatc cgccccgtcc
    10981 gctgaataaa gttgcctccg gcggcgaatt ggcgcgtatc agccttgcct tacaggttgt
    11041 tgccagccaa tatacccaag ttcccaccct gatttttgat gaggtcgata ccggtattgg
    11101 agggggagtg gctgaaatgg tcggcaaggc attacgtgcg ttgggcagaa aacatcaggt
    11161 gcttgccgtt acccatcttc cgcaagtcgc atcctgcggt gaaaaccact ggcgagtgcg
    11221 caagcacagc gagggagagc aaaccgtcag cgaaatcagt atattggatg aaatccaacg
    11281 ggtcgaagag gttgcccgta tgttgggcgg agaagtcatt accgatacga cgcggcaaca
    11341 tgcggcagaa ttgctgcaac ttgcgtcgaa aaatagttta ttttaaaatc aatcagttaa
    11401 aaaataacta aaaataaaag tctaaaacaa tagacagaac tcagataaat ccgtattatt
    11461 acgctttctt aatcacttga acaagtgatt gtgctgcacc cgtagctcag ttggatagag
    11521 tatctggcta cgaaccagag ggtcgggcgt tcgaatcgct ccgggtgcgc cagtaagaaa
    11581 atacaatacg cgcccatcgt ctagcggtta ggacatcgcc ctttcacggc ggtaaccggg
    11641 gttcgattcc ccgtgggcgt gccaattcaa aatgcctccg attatatcgg aggcatttct
    11701 catttctcat ttctcatttc tcatttctca tttctcattt ctcatttctc atttctcatt
    11761 tctcatttct catttctcat ttctcatttc tcatttctca tttctcattt ctca
//
LOCUS       DAWHXS010000064        11690 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWHXS010000064 DAWHXS010000000
VERSION     DAWHXS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.064"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(120..1034)
                     /locus_tag="WIE22_001362"
     CDS             complement(120..1034)
                     /locus_tag="WIE22_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ4127438.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WIE22_001363"
     CDS             1290..2759
                     /locus_tag="WIE22_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ4127439.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WIE22_001364"
     CDS             3011..3382
                     /locus_tag="WIE22_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127440.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLDSSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WIE22_001365"
     CDS             3455..4864
                     /locus_tag="WIE22_001365"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ4127441.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAVFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSEHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDVAIPRPAVLKGLETLPQRV
                     QTAPNSADAVKGIIEQTLA"
     gene            4911..5705
                     /locus_tag="WIE22_001366"
     CDS             4911..5705
                     /locus_tag="WIE22_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127442.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSVGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            5893..6669
                     /locus_tag="WIE22_001367"
     CDS             5893..6669
                     /locus_tag="WIE22_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ4127443.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6745..6851
                     /locus_tag="WIE22_001368"
                     /pseudo
     CDS             6745..6851
                     /locus_tag="WIE22_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7440..9911)
                     /locus_tag="WIE22_001369"
     CDS             complement(7440..9911)
                     /locus_tag="WIE22_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ4127444.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9908..11086)
                     /gene="hflX"
                     /locus_tag="WIE22_001370"
     CDS             complement(9908..11086)
                     /gene="hflX"
                     /locus_tag="WIE22_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ4127445.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSADRLNGFQTAL
                     AEAVELVKAAGGDPVLVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLSSLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11091..>11690)
                     /locus_tag="WIE22_001371"
     CDS             complement(11091..>11690)
                     /locus_tag="WIE22_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ4127446.1"
                     /translation="PVGSGVLPYFYGERPDPMPGYEMTGNPVSIDFSEAAGKITMKSF
                     KLYQGKNEIRPVKVLTAGNDPNGRLTAYQFALFPIKPLEYGTLYTAVFDYVRNGRRAQ
                     AKWQFKTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRLGSRLS
                     IGRHEAGGIVFSVGGMTGSRITLAPEDSPERGVTLYLQD"
BASE COUNT         2621 a         3006 c         3241 g         2822 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ctttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgctga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaaag gatgaccggc aacgatgtga tgcagatgct
     3301 ggattcgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgtgttcaag gaaaaatacc atatcggtac ggtcaactcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgccggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttt tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acaaatgggc ttgcccatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cataccgccc gcgcaacagc gagcatactt atgttacctc
     4381 cagcccgtcg atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtggctgcag gcaaaggctt
     4501 tgacctgcga tttgccttgg acaaagtcgg tggcaaatac ggctttactt ccggcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt tgcccgcgaa gtgcgcgaag aaggtgaaac
     4681 ggtggtttgt ttagaaactg cgttggcagc gaaattcgat gcaaccatac gcgaagccgt
     4741 cggcgatgtc gccattccgc gccccgccgt gctgaaaggt ttggaaacat tgccgcagcg
     4801 cgtgcaaacc gcgccgaata gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgtccc gtgtgggcgg cattccgtcc gaacttaaga ttgaaagaaa atgaccttac
     4921 ctatgcagga aacccgtttt tctatactgc tggacgaact ggcggcaaaa caggaggcag
     4981 ccatcgctcc ccacctgctt gccgacggaa ccaaggtatg ggtacgcaaa gcaggcaggc
     5041 acaatgcgcg gtggcgttac gcgctgctcg gtatggttgc ccgatatttg aaactggggg
     5101 tgttgaagcc ggttcccagc ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc
     5161 tgtacgaatt gcgctcggtg gggatagccg ttcccgaatt gctcgccgtc aggaaaaatg
     5221 cccttatgtt cggcaatttg gcaggcatcc cgctcgatgc gcaaatccgc caagaagccg
     5281 aagccggaaa ggcggacgct tggcttgccg gtttggaagc cattgcacgc gtgcataaaa
     5341 aacagcagtt cctcagtcag gcgtttgcac gaaatatgat gtgggacgga aaaaacatca
     5401 gctttctaga ttttgaagac gacccttccg aagtcctgac catagcgcaa tgccaagccc
     5461 gcgattggct ctgctacatc cattcgaccg cgctgatatt gaaaaacggc ggactgctgg
     5521 aagcggctgc ggaaaaatgg ggcggcgtac tgtctgagca gcctgccgaa atacaaaggc
     5581 tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac ccccgctggg
     5641 gacgggacgc gctcaggctg gcagcctcga tttccctgat ttcccttgcc gatataccgc
     5701 cataagccgc cgcccgcccc gcgatgccgt ctgaagccct tcagacggca tataaccaac
     5761 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttgacttt atcccgaatc
     5821 gggcagacat tttcactctg ccgcacatcc cgaacaagca ctatccattt cttttactga
     5881 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
     5941 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
     6001 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgcgcata cagcgtcgcc
     6061 tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga cggtccgctg
     6121 accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc
     6181 ggaaccctgg ttgccggcga cctgaatccg ggcaaacacc tttacttgtt gagcaccggt
     6241 accggcatcg ctcctttctt gagcatcacc aaagaccccg aaatttacga gcaatttgaa
     6301 aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tggcgtacta cgaccgcttt
     6361 accaaagaat tgcccgaaca cgaatacctc ggtgacttgg ttaaagaaaa actgatttac
     6421 tacccgattg tttcgcgcga agaattcgaa caccacggtc gcctaaccga cctgatggtg
     6481 agcggcaaac tgtttgaaga catcggcctg cccaaaatca acccgcaaga cgaccgcgcc
     6541 atgctgtgcg gcagccccgc gatgctgaaa gatacctgca aagttttgga cgatttcgga
     6601 ctgaccgtct ctccgaaaac aggcgtacgc ggcgattacc tgattgagcg tgcgtttgta
     6661 gatcaataag ctgtttgaaa atcccgtctg aacggcacca gcaatcaaaa accccgaatc
     6721 ctgccaatca ggattcgggg tttttatagt gaattaaatt taaatcagga caaggcggcg
     6781 agccgcagac agtacaaata gtacggcaag gcgagccaac actgtaccgg tttaaattta
     6841 attcactata tagtgggtaa aaattagaaa tgcacatttc cgtcattcca acacaggtgg
     6901 gaatccggac ttacctgttt cattatcgtt tgaaaataaa agagaaacca aacatataga
     6961 ttcccgcttg cgcgggaatg acgacggtgt tttgccgtag ccaaagcaga aaaacatccc
     7021 tattgtcatt tcatcatgat atgacaatag gatgttaaaa attaattatc attatatgat
     7081 tgatatataa gaaaaactgt cccaataaga aatgttatgc agaaaataaa atgaaaagga
     7141 tggttttcat tgaaaatggt tgttctgcca ccgtcataca cagagccagt atttggaaaa
     7201 aaatgatcca tattgacatc attattccat ttgtcgcata aatccacttt ttaatttttt
     7261 ccacattaat ataatctctt atttaataaa atcaaaataa aaatcaaatg cctaacaatt
     7321 cgatctcaaa ttttgaacgc caacgtgttt gagcggtaac aatacatact ttaagtttgc
     7381 aattcttcat cgtcttattt ttgttttcat caactatacg tctaaagcaa cgccgtccgt
     7441 cattgttcgg acggcatttt ttcagactgc attttccccc gtttgtgaag gcgcagggcg
     7501 ttgcccaaaa ccgccagcga actgaagctc atacccagtg cggctatcca cggttggaca
     7561 tagcccaaaa cggcaagcgg tacggcaatg atattgtacg cgcccgccca tatcaggttt
     7621 tgccggataa tatggcgggt gcgccgcgcc tgatcgagca ggtgggcgac ggtacgcaaa
     7681 tcttcgttca ataacacaat gtccgcgccg tccctcgcaa tatccgtccc gcccgctgcg
     7741 gcggcggata cgtctgcctg cgccaaaacg ggcgcgtcgt tgatgccgtc gcctatcatc
     7801 agcacttttt tcccttcttt ttgcaaggct ttgacgtatt ccagtttgtc ctcgggcatg
     7861 gcttgggcgc ggtagtgcgc gacacccagg gcgcgtgcgg tttcggcaac ggcggtttcg
     7921 cggtcgccgc tgaggatgtg cagggtcagg tttttgcctg ccaactgccg caccgcctcc
     7981 gccgcgctgt ctttcaaggg gtcggtcagg tagaacacgg cttggaaacc gctttgactg
     8041 ccgaggtaaa ccgcgctgcc gccgccttct gtttgcggtt ctttacctga aatttcggcg
     8101 acataggatg ccctgcccaa tgcccaaacc tgtgtttcgc cgttgacggt cagttgcgcg
     8161 cccacgcctt cgccgatgcg gttgaggcgt tgtttaatag cgatgtcggg gacgctgccg
     8221 tctgaaatgc ggcagttgag gatggcgcgg gcaaggggat gttcggactg ttgttctaaa
     8281 gcctgcgcca ccgcgagaac aaaagcttcg tctgtgcctc tcaacaatga gatacggcgg
     8341 acggcgggtt tgccttgggt cagcgtgccg gttttgtcga agatgatgtc ggtggtttgg
     8401 gcgagggttt cgattgcctg ctttccgccg attaaaatac cttcgcgcgc cagcgtaccg
     8461 gtagaagctg ccagcgcggt cggcgtggca agcgataagg cgcaggggca ggtaatgacc
     8521 agcagggcga cggtaatcca caatgcggtg tgcgcgtcgg cgtacagcgt ccagccgatg
     8581 aagacgggga cggcaagcag gagttcgccg aatatgaaag acgaggcgta ttgttccgcc
     8641 aactcggcag tgcgcggttt ttgcgctaag gcgcggtcga gcaggcggac gatgtgcgac
     8701 agtcgcgtgc caccgccggt gcggtcggtg cgtataatca gggggctttg cgtgttgagt
     8761 gtgccggcgg ttactttttc agacggcatt ttggcgacgg gcaggctctc gccggtcagc
     8821 atagattcgt tgacggcact gcttccttcc agcaccgtgc cgtcaacggg gatggtttcg
     8881 cccggtttga ccagcacgat atcgcccgcc ttcaatttga cgacagctgc ctcgcaggtt
     8941 tcctgcgtat cggggtaatc gggcatatga tggcaaaacg caggaatcag cttcaccagc
     9001 ctctcggcgg catcgcctgc cttacggcgg gcaatgtgtt ccataaagcg tccgcccagc
     9061 aggaaaaaca gcagcatcgc gatggattcg aaatacatcc cctgccccgc atttgtcgca
     9121 aggctgtaaa cgccggcgat aaaggtcatg atgatggcga cggtaatcgg cgtatccatg
     9181 ccgacgcggc ggtttttcaa gtcgcgcagc gcgccttgat aaaacgggac ggcgcaatag
     9241 aatacgacgg gcagcaccat taaaaagccg ccccaatgga ggatttgcag gaaatcgggt
     9301 tcgatgtcgc cgccgtaaag gtaggtcggc agcgcgaaca tcatcgtctg catcatcccc
     9361 agcccggcaa cggcgaggcg gacgatgtat tgtttgcgtt ctttttggtt ggcggcttcg
     9421 attttttgcg cgtcataggg tgcggcggtg tagcctatct gcctgatttt caacagaatg
     9481 tcggaaaggc ggattttgcc gtcgtcccag acgacgcggc agcggtgcgt gctgtaattg
     9541 aggtcgatgc ggacgatgcc gtctgtacgc aaaagctgct gttcgatcag ccagacgcag
     9601 gcggcgcagg tgatgccgcc gagcattaaa accgcctcgc gcgtgccgcc gtgggtttcc
     9661 acaaagtcgg actggacttc gggcaggtcg tacaggcgga tttggtcgag gatttcttgg
     9721 ggcggcagct cggttttttg cgcgtcggcg gtgcgttgtt tgtaataact gcccaagccc
     9781 gcgtcaataa tgctttgtgc gaccgcctga cagccggcgc agcaggtttc gcggtcttcg
     9841 ttttcgtaac ggacagtcag gtggaggtgt tcgggaacat ccagaccgca gtggaaacag
     9901 gtttttttca tggcatttcg gtttcgtctg tgtttggtgc ggcggcacaa gactcggcaa
     9961 tggcttcgcg cagtgcgtct ataccggtat tttcagcaac ggaaatgcgg acggcggcaa
    10021 tttttcccgc agcgtcgcgc catatgcccg tgttttgttc ttcagacggc agcaggtcgg
    10081 ttttgttgta caccttgatg cacggaatat cgccggcatg gatttcttgc agtacgtttt
    10141 ccacgtcttc aatctgctgt ccgctgttcg gagcggcggc atcgacgacg tgcagcagca
    10201 catcggcttg cgcggtttct tccagcgtgg cggaaaaggc ggaaatcagt ttgtgcggca
    10261 gatcgctgac gaatccgacg gtatcggtca ggataatgct gcattcggga ctgatgtaca
    10321 gccgccgcgc cgtcgtgtcg agtgtggcga aaagctggtc tttcgcatat atgcccgact
    10381 tggtcagccg gttgaacagg ctggatttgc cgacattggt atagccgacc agcgcaaacg
    10441 ttttgattgt gcccgattcg cgggacttgc ggcgcagggc gcgctgtttt ttgaggctgg
    10501 aaagctgttt tttcaaggcg ttgattcgat gggcgatcaa tcggcggtcg gtttccagtt
    10561 tggtttcgcc cggccctttc atgccgatgc cgccgcgctg gctttgcagg tgtccgtaac
    10621 cgcgtatcaa gcgtcccgcc aaatggctca actgcgccaa ttcgacttgc agcctgcctt
    10681 cctgcgtgcg ggcacggcgg gcaaaaatcg ccaaaatcag ccctaccctg tccaacacgc
    10741 ggcattggag gattttttcc aaattgcgct cctgcgtggg cgtgagttcg tggttgaata
    10801 cgaccaaatc gatgccgtct gcggcaactg cttctgacag ctccgccgcc ttgcccgtac
    10861 cgacaaacag cgcggtgtgc gggcggtcgc gtttggcggt ttccacaagg acgggatcgc
    10921 cgcctgccgc tttgaccagc tcgacggctt ccgccaatgc cgtctgaaaa ccgttcagac
    10981 ggtcggcact gctgcccgta ccatctttgt ccaacattac gcccaccagc atgacgcgtt
    11041 cgggttgcgc ttgggtactg ccgttgcggc ctgttctgcc tgtcatgtat tcaatcctgt
    11101 aaataaaggg taacgccgcg ttccgggctg tcttccggtg caagcgtgat gcggctgccc
    11161 gtcattccgc caacgctgaa gacgatgccg cccgcctcgt gccgtcctat ggacaggcgg
    11221 ctgccgagcc gctgccggta ggtataacgg gtacacgctt ccagacacca gcgtccgcgc
    11281 cagtgaatga aatatttttc gccttttcta accgcaagcg tctcgccgcc gtttacctca
    11341 aaataagggt aatcgggttt tcgggtttta aactgccatt tcgcctgcgc gcgccgtccg
    11401 ttgcggacat agtcgaatac cgccgtataa agcgtaccgt actccaaagg cttgatcggg
    11461 aaaagcgcga attggtacgc cgtcagcctg ccgttggggt cgttgccggc ggttaaaact
    11521 ttgacgggac ggatttcgtt tttaccctga tacagcttga aacttttcat cgtaattttg
    11581 cctgccgcct cagaaaaatc aatgctgaca ggattgcccg tcatttcata tcccggcatg
    11641 gggtcgggac gctccccgta aaaataaggc agcacgccgc tgccgactgg
//
LOCUS       DAWHXS010000065        11541 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWHXS010000065 DAWHXS010000000
VERSION     DAWHXS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.065"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            79..897
                     /gene="opc"
                     /locus_tag="WIE22_001372"
                     /gene_synonym="opcA"
     CDS             79..897
                     /gene="opc"
                     /locus_tag="WIE22_001372"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ4127447.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLKSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVPTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1060..1704
                     /locus_tag="WIE22_001373"
     CDS             1060..1704
                     /locus_tag="WIE22_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ4127448.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1723..3633
                     /locus_tag="WIE22_001374"
     CDS             1723..3633
                     /locus_tag="WIE22_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ4127449.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGKIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENETVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            3654..4019
                     /locus_tag="WIE22_001375"
     CDS             3654..4019
                     /locus_tag="WIE22_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127450.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4130..5605
                     /locus_tag="WIE22_001376"
     CDS             4130..5605
                     /locus_tag="WIE22_001376"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ4127451.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEQEKRGIYGGAVGVWDFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGIVADSDPTSEWQETQNKARAVIRAAQMVQEGLDK"
     gene            5602..6147
                     /locus_tag="WIE22_001377"
     CDS             5602..6147
                     /locus_tag="WIE22_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ4127452.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            6144..7280
                     /locus_tag="WIE22_001378"
     CDS             6144..7280
                     /locus_tag="WIE22_001378"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ4127453.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADRHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWLPLQS
                     HPNAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7327..7809
                     /locus_tag="WIE22_001379"
     CDS             7327..7809
                     /locus_tag="WIE22_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ4127454.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            7915..8970
                     /locus_tag="WIE22_001380"
     CDS             7915..8970
                     /locus_tag="WIE22_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4127455.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            8967..9161
                     /locus_tag="WIE22_001381"
     CDS             8967..9161
                     /locus_tag="WIE22_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127456.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALHQRGA"
     gene            9552..10157
                     /locus_tag="WIE22_001382"
     CDS             9552..10157
                     /locus_tag="WIE22_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ4127457.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            10220..10582
                     /gene="ssrA"
                     /locus_tag="WIE22_001383"
     tmRNA           10220..10582
                     /gene="ssrA"
                     /locus_tag="WIE22_001383"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(10899..10979)
                     /locus_tag="WIE22_001384"
                     /pseudo
     CDS             complement(10899..10979)
                     /locus_tag="WIE22_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3229 a         3213 c         2533 g         2566 t
ORIGIN      
        1 aatataattg taagcgtaac gattatttac gttatgttac catatccgac tacaatccaa
       61 attttggaga ttttaactat gaaaaaaaca gtttttacat gtgccatgat tgccctgacc
      121 ggtactgccg ccgctgcaca agagcttcaa accgctaatg agtttaccgt ccacaccgac
      181 ctctcttcca tttcttcaac tcgtgctttc ctgaaagaaa aacacaaagc tgccaaacac
      241 atcggcgtac gtgctgatat tccttttgat gccaaccaag gcatccgctt ggaagccggt
      301 ttcgggcgca gcaaaaaaaa tattattaat ttggaaacag atgagaacaa gctgggtaag
      361 actaaaaatg taaaactgcc caccggcgtt cctgaaaacc gtatcgatct ttacacaggc
      421 tacacctaca cccaaacgtt aagtgattct ttaaatttcc gtgtgggtgc cggcttgggt
      481 tttgaatctt caaaagacag cattaaaacc accaagcata cgcttcacag cagccgtcag
      541 tcgtggtcag ccaaagttca cgcggatttg ctttcccaac tgggtaacgg ctggtatatc
      601 aacccttggt ctgaagtgaa atttgacctc aagtcccgct ataaattaaa caccggcgtt
      661 accagtctca aaaaagacat caatcaaaaa accaacggct ggggctttgg attgggtgca
      721 aatattggta aaaaactggg cgaatccgcc agcatcgagg cggggccgtt ctacaaacaa
      781 cgtacttaca aagaatccgg cgagtttagt gtaccaacca agagtggcga cgtatcgctc
      841 accatcccga aaaccagtat tcgtgaatac ggcttgcgcg tcggcataaa attctgatga
      901 tttgaaatca tcattgtcgc tttaaatgcc aaaccgcaaa ctattttggt ttgcggtttt
      961 tacgtgaaat gaatttgaat agccgatgcc gtctgaaaat ttcagacggc atttttccaa
     1021 tcggttaaaa tacgcggttt gtttttttag gaacgcgccg tgcttgcctc cgccatcgac
     1081 ttcatcctcc atatcgacca acacctgctc gcgctgtcgg cgcaatacgg tgtgtggatt
     1141 tatgcgattc tgtttttgat tgttttttgc gaaaccggcc tgattgttac gcccttactg
     1201 cccggcgatt cgctgctgtt tgcggccggc ggtattgccg cgctgggcgg tatggatatt
     1261 catttgatgg ttgcattatt aagccttgcc gccatactcg gcgacgcgct caattttacc
     1321 gtcggcaaat atttcggcgg caggctgttc gccaatcccg attctaaaat cttccgacgc
     1381 gaatatctcg ataaaaccca ccgtttttac gaaaaacacg gcggcaaaac gattatcatc
     1441 gcgcgcttcg tgcccatcgt ccgcaccttc gccccctttg tcgccggtat ggggaaaatg
     1501 cactatgcca aatttatccg ctacaacatc atcggtggct tgttgtgggt tatcctgttt
     1561 tcttacgcgg gctatttctt tgccaacttc cccgtcgtaa aaaacaatct gggcttggtg
     1621 atgggcggca tcattattgt ttccgtcctg ccgggtgcgg ttgaaattgc ccgcgccaaa
     1681 cttgcggtaa aatccaaacg gtaactgttg agaatttgta tcatgaatat attatctgta
     1741 gaaaacgctt cttttgccat cggccacgtt gccttgctcg acaaaacttc ttttcaactc
     1801 gacagcggcg aaaaagtcgg cttgatcgga cgcaacggcg caggcaagtc ttcgttttta
     1861 aaaatcctcg ccggcgtgca aaagctcgac gacgggcaga ttattgttca aaacaacctc
     1921 aaaatcgttt atgtaccgca ggaatccttt tttgataagg acgcaaccgt atttgatacc
     1981 gttgccgaag gtttgggtaa aattcgcgat ttattgcgcc gttaccaccg cgtcggtcat
     2041 gagttggaaa acggttcggg tgaggctttg ttgaaagaac tcaacgaatt acaacttgaa
     2101 atcgaagcga aggacggctg gaagctggat gcggcagtca agcagacttt gggcgaactc
     2161 ggtttgccgg aaaacgaaaa aatcggcaac ctttccggcg gtcagaaaaa gcgtgtcgcc
     2221 ttggcgcagg cttgggtgca gaagcccgac gtattgctgc tggacgaacc gaccaaccat
     2281 ttggatattg acgcgattat ctggttggaa aacctgctca aggcgtttga aggcagcttg
     2341 gtcgtgatta cccacgaccg ccgttttttg gataatatcg ctacgcggat tgtcgaactt
     2401 gaccgcggca ttctacgttc ctatcccggc tcgttctcta aatacagtga gaaaaaagcg
     2461 caagagttgg cagtcgaggc ggaacataac cgcttgtttg acaaatttca tgctcaggaa
     2521 gaagcgtgga tacgcaaagg catcgaagcg cgacgtaccc gtaacgaagg acgcgtgcgc
     2581 cgtttggaag agctgcgccg ccaacgtgca gaacgccgca acgtgcaggg acaggtcaac
     2641 tttaagctcg atagcggtga gaaaagcggc aaaatcatcg ccgaattgga acacgcttcg
     2701 tttgcctatg gcggcaaagt cattatggac aaattctccg ccatcttgca gcgtggcgac
     2761 aaaatcggct taatcggccc aaacggtatc ggcaaaacca ccttcctcaa gctgattttg
     2821 ggcgaattgc agccgaccta cggcagaatc cgtatcggca gcaagcagga agtcgcctat
     2881 ttcgaccagt tccgcagcgc gttgaatgaa aacgaaaccg tgttttacac gctcggacag
     2941 ggcaatgatt acgttgaagt cggcggtaag aaaaagcatg tgatgagcta tttagaagat
     3001 ttcctgttcc cccctgcccg cgcgcaaagt cctgtttctt cactctctgg cggcgaacgc
     3061 aaccgccttt tgctggcaaa actctttacc cgtcctgcta atattttgat tttggacgaa
     3121 ccgaccaacg acttggatat cgatacccaa gagctgcttg aagatttgct gcgcgattat
     3181 caaggcacgg tattccttgt ctcacacgac cgtatgttcc tcgataacgt gattacccaa
     3241 agcattattt ttgaaggaaa tggaaggtta aaagaatata tcggcggcta tcaggactac
     3301 atcgacgcaa aatcacgcga agcgaagatt cagacggaat ccgcacccaa aacagccgat
     3361 gccgaaccgg tgaaagaaaa acccaaagcc aaccgcacgg tcaaactgtc ctacaaagaa
     3421 cagcgcgaac tcgacgccct gcccgacgaa atcgccgcct tggaagccga acaggcagaa
     3481 atcaacgcgc agctttccga tcccgaaatc ttcaaagatt acgaaaaagc cggcgcactg
     3541 caaagccgcg ccgaagaaat cgaaatgctg cttttggaaa agctggaacg ctgggagctg
     3601 ctggaaacca aacagaacgg caatgccgtc tgaaatcaaa caggaagaaa aaaatgttca
     3661 aacgacctga agaaatcatt gtcctgatac ttgccgtcct ctggattgcc ggtacgtatt
     3721 ttctcgccgc cctgtttgga gcggatgctt ataccgtgtt aaaaatcact gcccttaccc
     3781 tgctttggtc tgccgcatca ttcctccttt ggcaaaaaaa cctcagccgg catatctggc
     3841 cgctgctgct cggctgcctg actgcctgtt ggtggccgta tctgaatcaa tcggtcgcac
     3901 ccggttcttt accttggtat gcatcatgga cgttcaaaac catcttagcc ctgattcccg
     3961 taaccgcagg ttatctgttt aaatggaaac aataccggca gcgcaaaaca aagccgtaag
     4021 cccgtccttt tcagacggca tctccggtca gactgtcaac aattccgata aaatgctata
     4081 ctcgcgcccg ttgccgcgcc gtccgaaacc gccctttggg aaagcagaaa tgatcagcaa
     4141 acaagaatac caagcccaag ccgcccaagg ctacaaccgc atcccgctcg tgcaagaact
     4201 ccttgccgat ttggatacgc cgctttccct ctatctcaaa ctcgccaacc gcccctatac
     4261 ctacctgctc gaatccgttg tcggtggcga acgtttcggc cgctattctt ttatcggcct
     4321 gccttgcagc cactatctca aagccagcgg caaacacgtc gatgtttatc aaaacggcga
     4381 aattgtcgag caacacgacg gcaaccccct gccctttatc gaagccttcc acaaccgctt
     4441 caaaacaccc gaaatcccaa gcctgccgcg ctttaccggc ggactggtcg gctacttcgg
     4501 ttacgaaacc atctacaatt ttgaacactt cgcccaccgc ctgaaaaaca ccaccaaagc
     4561 cgacccgctc ggcacgcccg acatcttgct gatgctgtcg caagaattgg cggtgatcga
     4621 caatttgagc ggcaaaatcc atctcgtcgt ttacgccgat ccgtcgcagc ccgacggcta
     4681 cgaacgcgcc cgcgaacgcc tcgaagacat ccgtacacag ttacgccaaa gttgcgccat
     4741 cccgctttcg ctcggcagca aacacaccga agccgtcagc gaattcggcg aagagccgtt
     4801 caaagcctgc gttaacaaaa tcaaagacta catctttgca ggcgactgta tgcaggttgt
     4861 ccccagccag cgcatgagca tggaatttac cgacagcccg ctcgccctct accgcgccct
     4921 gcgtacgctc aacccttcgc cttatctctt ttactacgat ttcggcgatt tccacatcgt
     4981 cggctcctcg cccgaaatcc ttgtccgccg cgaacgcgac gatgtcatcg tccgccccat
     5041 cgccggcacg cgcctgcgcg gcaaaacccc cgccgaagac cttgccaacg aacaagattt
     5101 gttaagcgat gccaaagaaa tcgccgaaca cgtcatgctc atcgatttag ggcgcaacga
     5161 cgtcggccgc atcagcaaaa cgggcgaagt caaagtaacc gataaaatgg tgattgaaaa
     5221 atactcccat gtgatgcaca tcgtttccaa cgtcgaaggc cgtctgaaag agggcgttac
     5281 caacatggac atcctcgccg ctaccttccc cgcaggcaca ctctccggcg cacccaaagt
     5341 ccgcgccatg gaaatcatcg aagaagtcga acaggagaaa cgcggcatct acggcggcgc
     5401 ggtcggcgta tgggacttca acaacgacat ggatttggca atcgccatcc gcaccgccgt
     5461 ggtgaaaaac aacacgctat acgtccaaag cggcgcaggt atcgtcgccg actccgaccc
     5521 gacctccgaa tggcaggaaa cacaaaacaa agcccgagcc gttatacgcg ccgcgcagat
     5581 ggtgcaggaa gggttggata aatgacttat gaaaaacttt atgagcttcg ccaaacactc
     5641 agaccgacta ccaaagacgg tctctataca gataatgaaa aaagtcgaga aatcctaacg
     5701 gttagatgga gcggcaacac agaaaatcct aaaaatttct cagctgttgc tataggaatt
     5761 aatccaagta aagcaaatga tgaaagaagc gacaaaaccc ttacccaact tgcacgtttc
     5821 ttggatatgt acggttttac caacttcaaa atgttaaata ttttttccag ttactctaca
     5881 cagcaaacag gaattagggc aaacacccaa accgattttt ccaaatttaa aggatgtttg
     5941 gaagatgctg atatgattat tcttgcatgg ggtacagacc gcggtgctta taaggatgaa
     6001 aaaaacagaa tactggaatt tctaaaagca gaaaaattta tggaaaaagt attctgtatt
     6061 tctgaaactg gtaattccag cgatacaaga catccgtcaa gaattagtta tagctatcaa
     6121 cttgtacaat ttgaagagtc agcatgaaaa acatatctct ttctccgccc gccatgcttg
     6181 gcatcctcgg cggcggacaa ttaggcagaa tgtttaccgt tgccgccaaa accatgggct
     6241 acaaagtaac cgttctcgac cccgatccgg acgcgccggc agcagaattt gccgaccgcc
     6301 atttgtgcgc gccgtttaac gaccaagctg ctttggacga attggcaaaa tgcgcggcgg
     6361 tgaccactga atttgaaaac gtcaatgccg atgcgatgcg ctttttggca aaacatacca
     6421 atgtttcccc tagcggcgat tgtgtggcga ttgcacaaaa ccgcattcag gaaaaggcat
     6481 ggatacgcaa agcgggattg caaaccgcgc cgtatcaagt ggtttgtaag gctgaagaca
     6541 tcactgaagc aagcgcgcaa tttttgcccg gcatcctgaa aacggctacg ttgggctacg
     6601 acggcaaagg tcaaatccgc gtaaaaacat tggatgaact caaagccgcg tttgccgaac
     6661 acggcggcgt ggattgcgtt ttggaaaaaa tggtggattt gcgcagtgaa atttccgtaa
     6721 tcgtatgccg tttgaacaat gacaacgtgc aaactttcga ccctgccgaa aacatccacg
     6781 aaaacggcat cttggcttat tccatcgtcc ccgcgcgact gagtgccgac gtgcagcaac
     6841 aggcgcggca gatggcgcaa cgcttggcgg acgaattgga ttatgtcggc gtattggcgg
     6901 tagaaatgtt tgttgtcggt gacacgcatg aattggtcgt caacgaaatc gccccgcgcc
     6961 cgcacaattc cggacaccat acgatagatg cctgcgcagc agaccagttc cagcagcagg
     7021 tacgcattat gtgcaacctg ccgcctgccg ataccaaatt actgagttct tgctgtatgg
     7081 caaatatttt gggcgacgtt tggcaggaag acggcggcga accggattgg ctgcccttgc
     7141 aaagccatcc gaatgcacac ctgcaccttt acggcaaaaa aaccgcgcac aaagggcgga
     7201 aaatgggaca ctttaccgtt ttaaccaccg attcggacac cgcatttcaa gaagcaaaaa
     7261 aactgcatca gtccctataa tccgaccaac ggcacagatg ccgtctgaag ccgccgacat
     7321 accgccatga gcctgctgac actgttacgc cccgccaccg tgcaagactg caaagacatc
     7381 ttcaaagtcc acctgcactc cgtccaatac acctgcatcc tcagctacaa cgaacacgcc
     7441 ctaaaagtat gggaaggctt gctaaacaca gaaagctacc tgccgaccat ttccgatccc
     7501 gacaaagcac tttgggttgc cgaatacaaa ggcaacatac agggattttt ccaaatcgac
     7561 tgccaagaag cacaactcga tgcactttac gtccaccccc tgttccacaa cctcggactg
     7621 ggcactgccc tacttcatca ggcggaaacc atcgcccaca aatccggttt gagctttctg
     7681 aaactctacg catcgctcaa ctccgtcccg ttttaccttc tcaaccgcta cgaatcgctc
     7741 ggttcggcag tattgcagct tgaccccagc atcaaaatca aatgcgaact gatgcggaaa
     7801 cacctctgat ccgtccttat aaccaaaact gataatcttc aaatcaaaca ttatttccac
     7861 aagcgcagac agccgtataa agttgcccca tctaccaacc gacaggacaa gcatatgaaa
     7921 acctacgcac cggcactcta taccgcagcc ctgctcaccg cctgcagccc cgcagccgat
     7981 tcaaatcatc cgtccggaca aaatgccccg gccaataccg aatccgacgg aaaaaacatt
     8041 accctgctca acgcctcata cgatgtggca cgggattttt acaaagaata caacccctta
     8101 tttatcaaaa cataccaatc cgaacacccc ggcacatccg tcagcatcca acagtcccac
     8161 ggcggctcca gcaaacaggc attatccgta gccaacggcc ttcaagccga tgtcgtaacc
     8221 atgaaccaat cctccgacat cgacctgctc gaaaaaaaag gactggtaga aaaaggctgg
     8281 caacaagccc tccccgacca cgccgcgccc tacaccagca ctatggtttt ccttgtccga
     8341 aaaaacaacc ccaaacagat ccgcgattgg aacgaccttg ccaaagacgg cgttaacatc
     8401 gtcatcgcca atcccaaaac ctcgggcaac ggacgctacg ccttcctcgg cgcatacggt
     8461 tacggtctga aagccaccaa cggcaacgaa caggaagccc aaaaactcgt cgcatccatc
     8521 ctcaaaaaca cccccgtttt tgaaaacggc ggacgcgccg ccaccaccac cttcacacaa
     8581 cgcaacatcg gcgacgtact cattactttt gaaaacgaag ccaactacgt cagcaaaaaa
     8641 ctgacccaag gacagtttga aatcgtctat ccgagctaca ccatttccgc cgaaagcccc
     8701 gtcgccgtcg tcaacagcat cgtcgcgaaa aaaggcacgc aaaaaaccgc ccgcgcctat
     8761 ctcgaatacc tttggagcga accggcgcaa gaactcgccg cctcactcta cctgcgcccc
     8821 cgcaaccccg aagtattggc aagacacaaa gccgacttcc cagacttaga cacattctct
     8881 cccgaagaaa aattcggcgg atgggacaac atcatgaaaa cctacttcgc cgacggcggc
     8941 atattcgacc gcctgaccgc acaaaaatga ccgctatgcc gtctgaaccg cctttcagac
     9001 ggcattccac acatcaaaac agaaaaaccg cgcccaaaat catcatcaca agacacaacg
     9061 gcaaaccgga tacaaaaccc aacaccgccg ccaaaatgcc gtctgaagcc gtcggcgcaa
     9121 ccatccttat ctggggcgcg cttcatcaaa ggggcgcata actttcctta accatccctt
     9181 ttgacagcca aataaacagt gcttttgcct gtcagaaaca aaaccaatat tttgcagttc
     9241 gtatcggaac atttataaaa catcagattt ccgtgtttcg cgtttccggc aattcaagat
     9301 tggcaaaaca cgtcagcctg cttcgcaagg caggttttta aaactttatc aggcgttcgg
     9361 gcatatcggt tcgaccggtc cgcatttccg gcaatcaaca ctgccgacgg ttgccgcatt
     9421 tctttttgat atacaatcac actttcaaaa aacatacgca ggcaacaaaa tcatcctccc
     9481 ccgattggta aacattccgg caaattaaaa ccccgcccga aacagccatc ggacaaataa
     9541 caggaaaaaa tatgaatacc gacttccaaa acatcctcaa acaactgggc aaacaagccc
     9601 aaaaagaagc ggcagaaaaa caggcagccg aaaaaaacaa gcaaaaacaa gaacaggatt
     9661 tcgatttttc acaagcagtc ggacaagtct ccccattaaa aaaccggcag caatattatg
     9721 cgccgcccga caaaaccccc atcaaagtcc gtcccaaaga caaccgcgcc gacgaagaaa
     9781 actacttcta catcggcagc acatacaacg acccgcccgc cagcttcagc aaaaacggac
     9841 agggaaaaaa cgacatccaa cgcctgaaaa acggatacta tcccgtcgtt accgatgtcg
     9901 acctgcacgg ctacacacag gaagaagccc aaaaagtcct caacgaattc atcgcattca
     9961 cccaaaaacg cggcgtatgc ggcgaaatca tccacggcag cggattaggt tccaaaggct
    10021 acaaacccgt tctgaaaaat atgacccgaa actggctgat gcaacacccc gacgtactcg
    10081 cctacgccga accccgggaa ggcaacgacg gctgcgtccg catcctgctc aaacgcaaat
    10141 taaggcagca agactgacaa atttctccga cagacttggc agcaccgcac aatatccgta
    10201 taatttgcaa accatttctg ggggcgacct tggtttcgac gagggttgcg aagcagatgc
    10261 gggcataccg gggtctcaga ttcccgtaaa acactgaatt caaatagtcg caaacgacga
    10321 aacttacgct ttagccgctt aaggctagcc gttgcagcag tcggtcaatg ggctgtgtgg
    10381 cgaaagccac cgcaacgtca tcttacattg actggtttcc taccgggtta cttggcagga
    10441 aataagactt aaggtaactg gtttccaaaa ggcctgttgg tcggcatgat ggaaataaga
    10501 ttttcaaata gacacaacta agtatgtaga acgctttgta gaggactttc ggacgggggt
    10561 tcgattcccc ccgcctccac cataccctat tttcagcagc atcctgcaac acaaaaaagc
    10621 cttgaacttc aacagtttca aggctttttt gtgttgcagg atgctggcgt atgcttgagt
    10681 tttcgccatt tatggggtaa cttttggggt aacttttcac gaattacccc aaaaaacgcc
    10741 ttaaaaatgc aaaagtcatc acgcattttt ttaaaaaggt tacccaaaac ttaaatgtaa
    10801 tcctttattt ataagcttgc agctttccaa acaagttacc cccaaacgga taaaaaaatg
    10861 ccgaagccga actgacccca aaaagtcgga cagtttaatc aagcggcttt cagggactga
    10921 attctgtact gaacaaggct cagccctttt aatttcaact tgattctatc gttgttgtat
    10981 agtggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc
    11041 tgcggcttca tcgccttgtc ctgatttaaa tttaatccac tataaaaaaa catctgatat
    11101 tgccaacctt gatccgaatg cagtatcggc ttttcagacg atcccggttt ctccggcgca
    11161 cctttcagta tctcgccggc caaatcgaaa gtcgggcgga tttgaatacg gtaactgacg
    11221 atttccccgt taaacaaatc cataatcgga gaaaggtata tcttttctcc gcctacattg
    11281 aactcggtaa cgtccgttac ccatttctca ttcggctttt ctgcatggaa acagcgtcgc
    11341 aggatattcg gcgcaatttt gccgacttct cctttgaacg agcggtattt gcgccgccgt
    11401 atcactgcct tcagccccgt cttcgccatc agacggctga ctttcttgtg attgaccggt
    11461 gttcctgcgt gacggatggc tgccgcaatc ctccggtagc cgtatcttcc cttatgtcgt
    11521 cgataaatat catgagacct t
//
LOCUS       DAWHXS010000066        10621 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWHXS010000066 DAWHXS010000000
VERSION     DAWHXS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.066"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(120..1337)
                     /locus_tag="WIE22_001385"
     CDS             complement(120..1337)
                     /locus_tag="WIE22_001385"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ4127458.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1539..2267)
                     /gene="rph"
                     /locus_tag="WIE22_001386"
     CDS             complement(1539..2267)
                     /gene="rph"
                     /locus_tag="WIE22_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ4127459.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2350..2814)
                     /locus_tag="WIE22_001387"
     CDS             complement(2350..2814)
                     /locus_tag="WIE22_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ4127460.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3031..3807
                     /locus_tag="WIE22_001388"
     CDS             3031..3807
                     /locus_tag="WIE22_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ4127461.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3943..4470
                     /locus_tag="WIE22_001389"
     CDS             3943..4470
                     /locus_tag="WIE22_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127462.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRSS
                     TDYIVSRNLKFRHLY"
     gene            4641..5495
                     /locus_tag="WIE22_001390"
     CDS             4641..5495
                     /locus_tag="WIE22_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ4127463.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5536..6744
                     /gene="pncB"
                     /locus_tag="WIE22_001391"
     CDS             5536..6744
                     /gene="pncB"
                     /locus_tag="WIE22_001391"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ4127464.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6836..8554
                     /gene="argS"
                     /locus_tag="WIE22_001392"
     CDS             6836..8554
                     /gene="argS"
                     /locus_tag="WIE22_001392"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ4127465.1"
                     /translation="MNLHQTVEREAAAAFTAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKTGEWDADAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9105..10523
                     /locus_tag="WIE22_001393"
     CDS             9105..10523
                     /locus_tag="WIE22_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ4127466.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIVGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVVAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGKM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2530 a         3015 c         2615 g         2461 t
ORIGIN      
        1 tttaatccac tatatttttt gttccaaagt caaaatatgc cgtccgaaca ttcgggcggc
       61 agacaaaacg gcactgcccg ataaaggcag tgccgttgtc cgtttcaaac cgtgaaacat
      121 cagcccaaat taaaggcttt atgcaatacc ctggttgcca gttccatgta tttttcatca
      181 atcaatacgg aaactttgat ttcggaggtg gaaatcattt ggatgttgat accctcttcg
      241 gcgagcgtgc ggaagatttt ggcggctaca ccgacgtgcg aacgcatacc caaaccgact
      301 gcggagactt tgcatacggt gtcgtcgcca tcaatagaag ccgcgccgat actgtcttgg
      361 cgttccgaca ggatttccaa agtctgcttg taatcgccgc gcggtacggt aaaggaaaaa
      421 tcggttgtgc cttcgctgcc gacattttgg ataatcatat cgacttcgat gttggcatcg
      481 gcaaccgcgc ctaaaatctg ataggcaacg ccgggtttgt cgggtacgcc gcgcacgttg
      541 atgcgggctt ggtttttatc gaatgcgata ccggttacgg cagctctttc catgttgtcg
      601 tcctcttcaa aggtaattaa ggtgccattg ccgccgtctt gcaggctgct caatacgcgc
      661 aggcgcactt tgtattttcc ggcgaattct actgaacgga tttgcaaaac tttcgaaccg
      721 aggcttgcca gttcgatcat ttcttcaaat gtaaccgtat ccatgcggcg cgcttcgggt
      781 acgacgcggg ggtcggttgt gtaaacgccg tctacgtcgg tatagatttg gcactcgtcg
      841 gctttgaggg ctgcggcaag cgcgacggcg gaagtgtcgg aaccgccgcg tccgagcgtg
      901 gaaatattgc cttcactgct gatgccttgg aagccggcaa cgatgacgac tttgcctgca
      961 tcgaggtcgg cacgcatttt ttcgtcatca atgctttcga tgcgggcttt ggtgtgggcg
     1021 gtatcggttt tgagggcgac ctgccagccg gtgtagcttt tggcttccac gccgatgtct
     1081 ttcaatgcca tcgccaaaag gccgatggtt acttgttcgc cggtggacaa aaccacgtcc
     1141 aattcgcgcg gatcgggatg ctcctgcatt tcgtgcgcca acgcgaccag gcggttggtt
     1201 tcaccgctca tggcggatac gacgacgaca atgtcgtgtc cttcggcgcg ggctttggcg
     1261 acacgtttgg ccacgttttt aatgcgttcg ggcgagccta cggatgtgcc gccgtatttg
     1321 tgtacgatta acgccatgtt tcgtgctttc ttgtgtggga ttggtcgggc agcatggttt
     1381 gctggaaaag cggctattat tactattttt tacatgaagt tcaagaacgg actgcgcttt
     1441 cccgcctgcc gtttgacagc ggtcagcgaa aaacctgttc tttcagattg ttgacaaaat
     1501 gccgtctgaa cggttttcag acggcatccg gacgacaatc aggcggcgga caacgcattt
     1561 tgctggtgtt gcagcagttc gcctatgcct ttttgcgcca gtgcaaccag tttgcccaat
     1621 tcgtccaaac tgaacggcgc gtcttccgcc gtaccttgga tttcgatgat ttttcccgat
     1681 gcggtcatga cgatattcac atcactgtcg caaccggagt cttcgggata atccaaatcc
     1741 aaaagcggca cgccgttcac tacgcctact gatacagcgg caacggcttc gcggatgggg
     1801 ttttcactca aaatgccgtc tgaaaccagt ttgccgacgg cgatttgcag cgcgacaaac
     1861 gcaccggtaa tcgaagccgt gcgcgtaccg ccatctgcct gaatcacatc gcagtcaatc
     1921 aagatttgtc gttcgccgag tttttccata tccacgaccg cgcgtagcga acgcccgata
     1981 aggcgttgga tttcttgtgt gcgcccggac tgtttgcccg ccgaagcttc gcggcgcata
     2041 cgcgaagcgg ttgaggcagg cagcatcccg tattccgccg ttacccagcc ttggttttta
     2101 ccgcgcaaaa acggcgggac gttttcatct atggaagcgg tacaaatcac tttggtattg
     2161 ccgcattcaa taaggcacga accgtccgta tgcggcagga aatgaggggt aattttgata
     2221 tcgcgcaggc tgtccgcggc gcgtgaggtg cggatgtaat caggcatact gccctcccgt
     2281 taaaaacaga taaattaaaa agcctcaaac atgaaaaact cacatttaag gccttcaaac
     2341 tgaaaatttc tacgcttctt cggctttgct gcggataatc aaaagcggca ggtggctttg
     2401 gcgcattacc gtttcggcga aactgcccat caaaaggtgc atcagcccag tacgtccgtg
     2461 cgtacccaac accagcaggt cggcaccgtt ttcatcggca taatcaacca aatcctgcgc
     2521 catttcacgc gcacccttat tggcaaccag caggtgtttg acggtatttt ccacacccag
     2581 ttcccgggcg gtgcgctcgg cggcatccaa aacttcgttg ccttgcgcga cggcggcggc
     2641 ttcgtagctt tcgtgttgca aaaattcggg ggcgagcgcc atatattcgg caggattggc
     2701 aacgtgtacc aaagtcagac gcgcaccgtt gaccccggca agctcggcgg catgtttcag
     2761 ggcattgatg gacgtttcac tgccgtcaac ggcaacaacc agatgtttgt acatatcgta
     2821 ttctcctttt gcaccgcctc gcggtgccct cttgtcggat gggcgcaggg acagtttgcg
     2881 ctgtttcatt atagacccgc cgtcgggctt tatacaacag ccgaacagcc cgaccgcttt
     2941 ccagtataat atgccgcttc cgtgcagtca ggcatttttt gccggctttc gttcactttt
     3001 tgatttgacg caatcttgca ggattcgacc atgtccgaca acgctttgac ctcttcgcga
     3061 cgcttcggcg gcatcgccag actctacgga gactctgcct tggcgcactt ttcacaggca
     3121 cacgtctgcg tagtcggcgt gggcggtgtc ggctcgtggg cggtcgaggc tttggcgcgg
     3181 acgggcatcg gacgtttgac tttgattgat ttggacaacg ttgccgaatc gaatgtcaac
     3241 cgccagctgc acgccctgac cggagacttc ggcaaagcaa aagttaccgc cttgcgcgaa
     3301 cgcgtcgccc aaatcaaccc agaatgccaa gtgtttgaaa ttgaagattt cgttaccgaa
     3361 gacaatttgc cggaatactt cggaaaaggt tttgatttcg tcatcgacgc gatcgaccaa
     3421 gtgcgcgtca aagcagcaat ggcggcttat tttgtggaac gcaaacaacc gtttgtcctc
     3481 agcggcggcg cgggcggaca aaaaaatccg gcgttaatcc aaaccgccga tttgagccgc
     3541 gtaacccacg acccgctgct tgccaacctg cgctacacct tgcggaaacg ctacggattc
     3601 agccgcgata cgaaagcaaa tatgcgcgtg ccttgcgtgt attcgaccga aaatatcgtg
     3661 ccgccgcagt ctagggaggc ttgttcggca gatgccgctc cgcaaggctt gtcgtgcgcc
     3721 ggctacggtg caagcatgct cgttaccgct tcgttcgggc tatattgcgc acaggcggcg
     3781 gtggaacaca tcgcaggcaa aaaataaaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacta tggttatgat ttaagacaac aaaggatacg gataaaaaat aacacaaaat
     3901 atatgattcc taataatata ccaagtatcg gagagctatt taatggaatt cgttaataat
     3961 ttagttattt tttcattttt attactaatg cttattccga tattttttgt agtatatggt
     4021 atataccata agatacgtta tcgcaaaata tgtatcctaa gaacaagttt tatattatta
     4081 gtggtaatac tttgcagtat gtattacata tattgccgtt atcttgacca acaaaaagta
     4141 gcttattatt gcatagatga acaatgtatt tctattgttc atctatacaa agattatggt
     4201 ataaactctc ccacatatgc gagaatttac gcaggaaaaa tattgtttag atttcaagta
     4261 agagctaaaa attacgctga attacttatg gaagatgata tatcaattag taaaaaaatt
     4321 ttggggaata aatttatcat ttatgggtcg ctacctgtaa tatacggtaa tgtagataat
     4381 attgaagtaa aagaagctac tggttatata gatagatcca gtactgatta tattgtctca
     4441 agaaacttaa aattcagaca tttatattaa ttaagaggtt ttagcaagac cgtattcaca
     4501 acctgctcct tttcaaaaca tttgcattta aaagccgtta taatgccgtc tgaacatctg
     4561 cccgaccaca ttatgcgtga atgccggcag attgttttct tttgtaaact tatattaaaa
     4621 tccacttacc gattcacgcc atgccgccca tccctgcccc atccgcacca tccgagcaca
     4681 ctgtcgcatg ggtattcggc caacccgtta ccgatttgcc ccaggatttg tttattccgc
     4741 ccgatgcatt gaaagtcgta ttgggcagtt ttcaaggccc tttggatcta ctgctgtatc
     4801 tgatccgcaa gcagaacatc gatgttctcg atattccgat ggtgaagatt accgagcagt
     4861 atctgcacta catcgcccaa atagaaacct atcagtttga tttggcggcg gaatatcttt
     4921 tgatggcagc aatgctgatt gaaatcaaat cgcgcctgct gctgccgcgt accgaagccg
     4981 tcgaagacga agaagccgac ccgcgtgccg agttggtgcg ccgcctgttg gcttatgaac
     5041 agatgaagct ggcggcacaa gggttggatg cgcttcctcg tgcgggccgg gatttcgcgt
     5101 gggcgtacct gcctctggaa attgccgtcg aagccaagct gcccgaagtc tatattaccg
     5161 acttgacgca agcgtggctg ggtattttgt ctcgggcaaa acacacgcgc agccacgaag
     5221 taatcaaaga aaccatctcc gtgcgcgcgc aaatgacggc aatcctgcgc cgtttgaacg
     5281 gacacgggat atgcaggttt cacgacctgt tcaatcccga acagggcgcg gcttacgtgg
     5341 tcgtcaactt catcgcactg ttggagcttg ccaaagaagg tttggtcgga atcgtacagg
     5401 aagtcggttt cggagaaatc cgaatcagcc tcaatcatga gggggcacat tcagacggca
     5461 tttccggcac acggggcggg cgcgatgtgt tctaatacgc cccaagccgc caccaaaaat
     5521 cgggagacac gccatatgac cggcatcata cattcgctgc ttgacaccga cctctacaaa
     5581 ttcactatgc tgcaagtagt cctgcaccag tttccgcaga cgcacagcct ttacgaattc
     5641 cgctgccgca acgcctcgac cgtctatccg cttgccgaca tcaaagaaga cttggaagtc
     5701 gaactcgacg cgctctgccg gctgcgcttc acccacgacg aactcggcta tctgcgctcc
     5761 ctgcgtttca tcaaaagcga ctttgtcgat tatctcgaac tcttccagct ccaacgccgc
     5821 tttgtcgaag tcggcacaga cgataaaggc cgtctgaaca tccgcatcga aggtccgatg
     5881 atacaggcga tgttctttga aatcttcatc ctcgccattg tcaacgaact ttacttccgc
     5941 cgcctggaaa cccctgcagt catagaagaa ggcgaacgcc gccttcaagc caaagccgcg
     6001 cgcctcaaag aaatcgccgc cgcacaaaac cccgacgaac cgcccttcct gatttccgac
     6061 ttcggcacgc gccgccgcta caagctcgcg tggcaggaac acgtcatccg caccctgctc
     6121 gaagccgccc caagcatcgt gcgcggcacc agcaatgtct ttctcgccaa aaaactcggc
     6181 atcaccccca tcggcaccat ggcgcacgaa ttcctccaag ccttccaggc cctcgacgta
     6241 cgcctgcgga atttccaaaa ggccgcgctc gaaagctggg tgcacgaata ccggggcgat
     6301 ttgggcgttg ccctgaccga cgtggtcggt atggatgcct tcctgcgcga tttcgacctc
     6361 tatttcgcca aacttttcga cgggctgcgc cacgacagcg gcgaccctta cgtttggggc
     6421 gacaaagcct acgcccacta tcaaaagctc aaaatcgaca gccgcaccaa aatgctgacc
     6481 ttctccgacg ggctggacat cgaacgctct tgggcattac accaatattt caaagaccgc
     6541 ttcaaaaccg gcttcggcat cggcaccaac ctcaccaacg atatggggca tacgcctttg
     6601 aatatcgtgt tgaaactggt cgaatgcaac gggcagtccg tcgccaagct gtccgactct
     6661 ccgggcaaaa ccatgaccaa caacagcacc ttcctcgcct acctgcgcca agtgttcgac
     6721 gtacccgaac ccgaaacgcc gtaaaccggc agaaaaagcg cacaattcct gtttctgccg
     6781 cataaaatct tttaaaatac cgcctgattt gaatttaacc gaaagaccga acttcatgaa
     6841 cctacatcaa accgtcgaac gcgaagccgc cgccgccttt accgccgcag gcatcgccga
     6901 cagtcccgtt gttttgcagc cgaccaaaaa tgccgaacac ggcgatttcc aaatcaacgg
     6961 cgtgatgggt gcggcgaaaa aagccaaaca aaacccgcgc gaactggcgc aaaaagtcgc
     7021 cgacgcattg gcaggcaacg ccgtcatcga aagcgcggaa gtcgccggtc cgggctttat
     7081 caacctgcgc ctgcgccccg aatttctcgc gcaaaacatt cagacggcct tgaacgacgc
     7141 gcgtttcggc gtggcaaaaa ccgacaaacc gcaaaccgtc gttatcgact attcctcgcc
     7201 caatctggca aaagaaatgc acgtcggcca tctgcgttcc agcatcatcg gcgacagcat
     7261 ttcgcgcgtg tcagaattta tgggcaatac cgttatccgt caaaaccacg tcggcgactg
     7321 gggtacgcag tttggcatgt tggtcgctta tttggtcgag cagcaaaaag acaatgccgc
     7381 attcgagctg gcggatttgg agcagtttta ccgcgccgcc aaagtgcgct ttgacgaaga
     7441 ccccgccttt gccgacaccg cgcgcgaata cgttgtgaag ctgcaaggcg gcgatgaaac
     7501 cgttttggca ttgtggaaac agtttgtcga tatttcgctc tcgcacgccc aagccgttta
     7561 cgacacgctg ggcttgaagc tgcgccccga agacgtggcg ggcgaatcga aatacaacga
     7621 cgatttgcag cccgtagtcg atgatttggt tcaaaaaggt ctggcagtcg aagacgacgg
     7681 cgcaaaagtc gtgttcttgg acgagtttaa aaacaaagag ggcgaacctg ccgcatttat
     7741 cgtgcaaaaa caaggcggcg gcttccttta tgcttccacc gacttggcgt gtttacgcta
     7801 ccgtgtcggc acgcttcatg ccgaccgcct gctgtacgtc gtcgaccacc gccaagccct
     7861 gcactttgag caactcttta ccacttcacg caaagcaggc tatctgccgg aaaacgtcgg
     7921 cgcggcattt gtcggcttcg gcacgatgat gggcaaagac ggcaaaccgt tcaaaacacg
     7981 cagcggcgac actgtaaaac tggtcgatct gctgaccgaa gccgtcgaac gcgccaccgc
     8041 tttggtgaaa gagaaaaatc ccgaattggg cgcggacgaa gccgccaaaa tcggcaaaac
     8101 cgtcggcatc ggcgcggtca aatacgccga cttgagcaaa aaccgcacca gcgattatgt
     8161 gttcgactgg gacgctatgc tctcgtttga aggcaacacc gccccctatc tgcaatacgc
     8221 ctacacccgc gtgcaaagcg tgttccgcaa aacaggcgaa tgggatgcgg acgcgccaac
     8281 cgttttgacc gaaccgctgg aaaaacagct tgccgccgag ctgctgaaat ttgaagacgt
     8341 actgcaaagc gtggcggaca cggcgtatcc gcactacctc gccgcctacc tctatcaaat
     8401 tgcgaccctg ttcagccgct tctacgaagc ctgtccgata ctcaaagccg aaggcgcaag
     8461 ccgcaacagc cgcctgcaac tggcaaaact caccggcgac acgctgaaac aaggcttgga
     8521 tttgctgggc atcgatgtgt tggacgtaat gtaaaaccgc accgcccgat tgcggacaac
     8581 agcctcgcca tccttatccg aatctgaaaa aagcggcgcg atacaccgta tccgccgccc
     8641 ctcccaaaat gcgaaacaaa caaacgccaa gcaagcaagc aagcaagcaa gcaagcaagc
     8701 aagcaagcaa gcaaaaaatt ataaccccct tcctgccgac gcacgcactt tccgcgcggc
     8761 gcattcccct ttttccgccc ctcaaacccg ccttttcttc aggcagggtt tcagcccgcc
     8821 ccctttccct gttttccttt ccccgacacg cgcgcgcgac ccctgccgca tcgtgccgca
     8881 ctttcgcgcc cggacggcat cgttccgcca tccggttctc tgtttcaaat acccctgttt
     8941 cagaaagaaa ttctagatgc ttcaacacac aggacgacat ataaagcgtc gccctatatg
     9001 ttgccctgat ttggaagggg ttacgcctcc caaataaagt ctgatcctgc cgccccgaag
     9061 gacagatgtc cgagtggcga agtttcaacc gaaaaggaaa tacgatgaat attcacaccc
     9121 tgctctccaa acaatggacg ctgccgccat tcctgccgaa acggctgctg ctgtccctgc
     9181 tgatactgct tgcccccaat gcggtgtttt gggttttggc actgctgacc gccaccgccc
     9241 gcccgattgt caatttggac taccttcccg ccgcgctgct gatcgccctg ccttggcgtt
     9301 tcgtcaaaat tgtcggcgta ttggcgtttt ggctggcggt tttgtttgac gggctgatga
     9361 tggtgatcca gcttttccct tttatggacc tcatcggcgc catcaacctc gtccccttca
     9421 tcctgaccgc ccccgcccct tatcagataa tgaccgggct gttgctgctg tatatgctgg
     9481 cgatgccgtt tgtgttgcag aaagccgccg ccaaaaccga cttccggcac attgccgtct
     9541 gcgccgccgt cgtggtggca gccggctatt tcaccggcca tttgagttac tacgaccggg
     9601 ggcggatggc caatatcttc ggcgcaaaca acttctatta cgccaaaagt caggcgatgc
     9661 tctacaccgt cagccagaat gccgacttta ttaccgccgg cctggtcgat cccgtcttcc
     9721 tccccttggg caatcagcag cgtgccgcca cgcatctgaa cgagccgaaa tctcaaaaaa
     9781 tcctctttat cgtcgccgaa tcttgggggc tgccggccaa tcccgagctt caaaacgcca
     9841 cttttgccaa actgctggcg caaaaagacc gtttttcggt ttgggaaagc ggcagttttc
     9901 cctttatcgg cgcgacggtc gaaggcaaaa tgcgcgaact gtgcgcctac ggcggtttgc
     9961 gcgggtttgc actgcgccgc gcgcccgacg aaaaatttgc ccgctgcctc cccaaccgtt
    10021 tgaaacaaga aggttacgcc acctttgcga tgcacggcgc gggcagttcg ctttacgacc
    10081 gcttcagctg gtatccgagg gcgggctttc aagaaatcaa aaccgccgaa aacctgatcg
    10141 gtaaaaaaac ctgcgccatt ttcggcggcg tgtgcgacag cgagctgttc ggcgaagtgt
    10201 cggcattttt caaaaaacac agcaaaggat tgttttactg gatgacgctg accagccacg
    10261 ccgactatcc cgaatccgac attttcaacc acaggctcaa atgcaccgaa tacggcctgc
    10321 ccgccgaaac cgacctctgc cgcaacttca gcctgcacac ccaatttttc gaccaactgg
    10381 cggatttaat ccaacgcccc gaaatgaaag gcacggaagt catcatcgtc ggcgaccatc
    10441 cgccgcccgt cggcaacctc aatgaaacct tccgctatct caaacagggg cacgtcgcct
    10501 ggctgcactt caaaatcaaa taacaacaat gccgtctgaa cgcaccaaca gccttcagac
    10561 ggcattttgc agacagaccg acccctcaag cccacttttt catcatttcc gataaattgc
    10621 t
//
LOCUS       DAWHXS010000067        10227 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWHXS010000067 DAWHXS010000000
VERSION     DAWHXS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.067"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..654)
                     /locus_tag="WIE22_001394"
     CDS             complement(<1..654)
                     /locus_tag="WIE22_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ4127467.1"
                     /translation="MKSLVIWLLLLGSAAGVFYHTQNQSLLVGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHELAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVNGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERACARRMQQPEAGRKYYRNACHNGAAVYADEVMP
                     "
     gene            complement(807..1532)
                     /locus_tag="WIE22_001395"
     CDS             complement(807..1532)
                     /locus_tag="WIE22_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ4127468.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASDIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMNQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(1605..2282)
                     /gene="radC"
                     /locus_tag="WIE22_001396"
     CDS             complement(1605..2282)
                     /gene="radC"
                     /locus_tag="WIE22_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ4127469.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQEGIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(2405..3754)
                     /gene="gshA"
                     /locus_tag="WIE22_001397"
     CDS             complement(2405..3754)
                     /gene="gshA"
                     /locus_tag="WIE22_001397"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ4127470.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDALGMPRSEKDCDVDNVHNRLYVYGVMARLSLLAASIELEETA"
     gene            4014..5423
                     /gene="leuC"
                     /locus_tag="WIE22_001398"
     CDS             4014..5423
                     /gene="leuC"
                     /locus_tag="WIE22_001398"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ4127471.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAILSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPKGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            5520..5774
                     /locus_tag="WIE22_001399"
     CDS             5520..5774
                     /locus_tag="WIE22_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127472.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            5836..6477
                     /gene="leuD"
                     /locus_tag="WIE22_001400"
     CDS             5836..6477
                     /gene="leuD"
                     /locus_tag="WIE22_001400"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ4127473.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            6652..7473
                     /gene="dcm"
                     /locus_tag="WIE22_001401"
     CDS             6652..7473
                     /gene="dcm"
                     /locus_tag="WIE22_001401"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4127474.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKNGLQKSA"
     gene            7488..7922
                     /locus_tag="WIE22_001402"
     CDS             7488..7922
                     /locus_tag="WIE22_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ4127475.1"
                     /translation="MPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVVVPVIKAVSERLLHTL"
     gene            7938..8268
                     /locus_tag="WIE22_001403"
                     /pseudo
     CDS             7938..8268
                     /locus_tag="WIE22_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
     gene            8302..9372
                     /gene="leuB"
                     /locus_tag="WIE22_001404"
     CDS             8302..9372
                     /gene="leuB"
                     /locus_tag="WIE22_001404"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ4127476.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            9535..10098
                     /locus_tag="WIE22_001405"
     CDS             9535..10098
                     /locus_tag="WIE22_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ4127477.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         2666 a         2791 c         2439 g         2331 t
ORIGIN      
        1 gggcatgact tcgtcggcat aaacggctgc accgttatgg caggcattac ggtaatattt
       61 ccgtcctgct tccggctgct gcattctccg ggcacaggcg cgctcaaacc tgccgttacc
      121 ttggttgaat acgagaacgg ttttaccgtt ttcgcgcaca aatgccgctc ctgcctcatc
      181 cgtatggcgg tcaagcaggg aaaggcggtg gtagattgcg ctcatcaatc cgttaacctg
      241 gcgttgctgc gtgcggatat cgctgtcgga cgattcggcg gcttcctctt ccgtgctgat
      301 gttttcatgt acgccattgt aaagataacc ggcaaggcgt gtgcgttcgg tcagcttttg
      361 tgcggtgtag tgcggattgt cgggatggtg ttcgccgtgt ccgtcttcgg gattaagcgt
      421 gagataacgt gcgtgcctgc gggcggagtt ttccaaaacc ggcgcgtgtg ccagctcgtg
      481 caaaccgatt tgtgcgcgga tgcggttgag gtagtgcagc gcgtcgccgc cgtccctgat
      541 ttgcggtgca gacggataga caagttctcc cacaagcagg gattggtttt gggtatggta
      601 gaaaacgcct gccgccgaac ccaacagaag cagccagata acaagggatt tcatgatgtc
      661 gccctctcgc cccggcaacg ggatatttca tgaaaacggc aaacttgaaa atcacgtttc
      721 cgcttgccga ttgccaaacc ggcaaacctt tccccgtgcc gatgccgaac ccgatgccgt
      781 ctgaaccttc agacggcatc aggtgtttat ttgtccaccc ggatgggtgc aggttcgtat
      841 tgtgtcgatt cgtcgccgta atacagcacg ccgagtttga tggggatgcg tccctgcgat
      901 ttgcggtggg cattggaatc gcgcaaggaa taggcgcagc cgcaatattc ctgctggtag
      961 aagttttcac gtttgctgat ttcaatcata cgcgcgctgc cgccgccttt gcgccagttg
     1021 aaatcccaat acaccacatc atcgtaaggc gcggcggcgc ggcggccgca gtcgttaatc
     1081 tggttcatgt ttttccaacg ggaaatgccc agcgagctgg tgaagacggg aaacccgtgt
     1141 tcatgcgcat attgcgccgc cttttcgaaa cgcatatcga aacacatggt gcagcggatg
     1201 ccgcgctcag gttcgaattc catacctttg gctttggcaa accattcttt gcggtcgttt
     1261 tcgtaatcgt cgtctttgtc gatgaagggg atgccgaact tttccgcaaa gcgtacgttt
     1321 tcctcttttc ggagcatata ttctttgtgc ggatggatat tgggattgta aaaataaatg
     1381 gtgtaatcaa tgtcgctggc cagcatggct tccatcactt cgccgctgca cggggcgcag
     1441 caggaatgca gcaagacttt tttatgtccg ccgggcggga cgagtatggg gcggtcaatg
     1501 tcggtaacgg taggtttgtt ttgtgtttcc attaacttaa caatatctgt cgaatacggt
     1561 tgaagcggct attttatcag agccgccgca tgtaaaacag ggtgtcaggg catcagcccg
     1621 agctgtctga acgaacggac ggtttgcgcc gttacgataa aatggtcgag cagcgacaca
     1681 tcgaccagcg acattgcctg tgccagccgc cttgtgaaca tgatgtcttc ctgcgaaggt
     1741 tcgggcgagc cgcccggatg gttgtgcgcg ataatcaggc tgtcggcata ttcgtccaat
     1801 gccagtttga cgatttctcg gatgtaaatc gtgttttccg caaccgtacc gcgcgacagc
     1861 tctctgaccg caatcagttg gttttggcgg ttcagcagca gtgcgacgct gacttcgact
     1921 ttttcctgtc ccaaatgaaa gcgcaggtaa tcggcaaccg tatcggggtc ggacaggacg
     1981 atgccctcct gcaattcttc cgccaatatc cgccgcccga tttccctgac cacggcgaac
     2041 tgtgtgaaac ttgccgtccc catccctttg tatgccgaca gtttgccgac ctccgcgctc
     2101 atcagcctcc ccaaactgcc gaactcctgc agcaaataac gcgccaaatc gaccgcactc
     2161 attccgcgcg tgccgacgcg cagcaggatt gccaaaagtt cggcatcact caaagccgcc
     2221 gccccgcgtt ccaacagctt ttccctgggc ctttcgcctt ccggccattg cttgatgctc
     2281 atgttttccc ttttttaaat agtctgagat gccgtctgaa acaaatgttc ccacacgcca
     2341 aatatccgaa atgccgtctg aaccggattt ccgcttcaga cggcatctgt atttcaaaac
     2401 agtcttacgc cgtttcttcc aactcgattg aagccgccag aagcgacagg cgtgccatca
     2461 cgccgtacac atagaggcgg ttgtggacat tatcgacatc gcagtctttt tcagaacgcg
     2521 gcatacccag cgcatcccat tgctcaaata cgcgcttgca ggcttcgggt gcttcttggg
     2581 aattatcgcc attgccggta ggaatgctat tggacagcgg cacaaacacc ataccgccgg
     2641 cgttgaggtt ttcgtccgca cctcgccctt cgtgtacgcg gaaaaagccg ccgatgacga
     2701 agcggtccat catatacacg acgggttcgc acaccgcgcc gtttaaggtt tcataagtat
     2761 aaatcccttc ttggacaatc acttcgctga cttccaagcc ttctttgact ttcgccattt
     2821 tattgcggtt tttacggttc aatccgcgca cttcgtcggc ggatttgacg ctcatcacgc
     2881 ccatgccgta agtgccggca tcggctttga cgatgacgaa aggtttgtcg gtaatgcccg
     2941 attcgtcgta tttggcttga attttcgcca gcacacgttc taccgcttcc gccaacgcgt
     3001 cttcgccttc acgcccttgg aagtccaaac cgccgatttt ttcaaaataa gggttaattt
     3061 gccattcgtc gatgccgatt aacttggcaa attcggcggt aacttggttg tacgcgccga
     3121 aatgatttgt tttgcggcgc gtcgtccaac cgccgtgcaa cggcggcaaa acggtttggc
     3181 tgatgccttt gaggatgtcg ggaatgccgg cagacaaatc gttgttcaac aaaaccacgc
     3241 aaggcgaaaa gccgtctgca agatggacgc ggtcgcgggt acgcaataaa ggttccaaca
     3301 ggattttgtc gcccaatgca gtttcaaatt cggtcggttc ggttacttcc ggattcaggc
     3361 tgcccaagcg cacttcatac cctgccgaac gcaaaatctc gccgagggcg taaatgtttt
     3421 gcaggtaaaa cgtattgcgc gtgtggtttt cgggaataat caatacggat ttcgccgttt
     3481 cgcaggcgcg ttgcaccgca tcttgcgcgg caaccgccgc cagcgggata aagttgggat
     3541 tcagattatt gaagccgccg gggaacaaat tcatatcgat agacgaaatt ttgtaaccgg
     3601 cattgcgtat atcgaccgaa ccgtaaaacg gcgggcggtg tacgctccac tgcgtgcgga
     3661 accacgcctc gattttggcg tgattggaca ggatttttgc ctcaaacgcc tgaagttgcg
     3721 ccgaatgttc gggcgacatc accggtaatt tcatctttcg tctgcctctg tgtgtttttt
     3781 gacaagatgc agatgataat gcctttgaaa gaatgacgac aagtattctt tcgtatcgaa
     3841 tcatgcttta gatataactg tttactgaaa tggacaatac gtctaatttt ttcgttaaaa
     3901 ttgtttgcaa aataacaccg gctcaaaaat tagacaaaat ttgttgcgcg gtataaagaa
     3961 tacgtctaaa atccgcccaa tccgcattac catttatcca aaagaacaac atcatgacag
     4021 cacaaaccct ctacgacaaa ctttggaaca gccacgtcgt ccgcgaagaa gaagacggca
     4081 ccgtcctgct ctacatcgac cgccatttgg tgcacgaagt taccagccct caggcatttg
     4141 aaggcttgaa aatggcgggg cgcaagctgt ggcgcatcga cagcgtcgtc tccaccgccg
     4201 accacaacac cccgaccggc gattgggaca aaggcatcca agacccgatt tccaagctgc
     4261 aagtcgatac tttggacaaa aacattaaag agtttggcgc actcgcctat tttccgttta
     4321 tggacaaagg tcagggcatc gtacacgtta tgggccccga acaaggcgcg accctgcccg
     4381 gtatgaccgt cgtctgcggc gactcgcaca cttccaccca cggtgcattc ggtgcgttgg
     4441 cacacggcat cggcacttcc gaagtcgagc acaccatggc aacccagtgt attaccgcga
     4501 aaaaatccaa atccatgctg attgccgttg acggcaaatt aaaagcgggc gttaccgcca
     4561 aagacgtggc actttacatc atcgggcaaa tcggcacggc aggcggtaca ggctacgcca
     4621 tcgagtttgg cggcgaagcc atcctcagcc tttctatgga aggccgcatg actttatgca
     4681 atatggcgat cgaagccggt gcgcgctcag gcatggttgc cgtcgaccaa accaccatcg
     4741 actatgtaaa agataaaccc ttcgcaccta aaggcgaagc atgggacaaa gccgtcgagt
     4801 actggcgcac gctggtgtct gacgaaggtg cggtattcga caaagaatac cgtttcaacg
     4861 ccgaagacat cgaaccgcaa gtcacttggg gtacctcgcc tgaaatggtt ttagacatca
     4921 gcagcaaagt gccgaatcct gccgaagaaa ccgatccggt caaacgcagc ggtatggaac
     4981 gcgcccttga atacatgggc ttggaagccg gtacgccatt aaacgaaatc cccgtcgaca
     5041 tcgtattcat cggctcttgc accaacagcc gcatcgaaga cttgcgcgaa gccgccgcca
     5101 tcgccaaaga ccgcaaaaaa gccgccaacg tacagcgcgt gttaatcgtc cccggctccg
     5161 gtttggttaa agaacaagcc gaaaaagaag gcttggacaa aattttcatc gaagccggtt
     5221 ttgaatggcg cgaaccgggc tgttcgatgt gtctcgccat gaacgccgac cgcctgaccc
     5281 cggggcaacg ctgcgcctcc acctccaacc gtaactttga aggccgtcaa ggcaacggcg
     5341 gacgtaccca cctcgtcagc cccgctatgg cagcagcagc cgcagtgacc ggccgcttta
     5401 ccgacatccg catgatggcg taagtatttt caggcggtct ttaagaattt gacgcggccg
     5461 tctgaaatat gtcccgtaag gcagaaacac ctttaatatt caaaccaagg agtttaacca
     5521 tgaacaaact tttcattacc gccctgtccg ccctcgcctt gtccgcctgc gccggcactt
     5581 gggagggcgc gaaacaagac accgcccgca accttgacaa aacacaggcc gccgccgaac
     5641 gcgccgccga acaaacaggc aacgccgtcg aaaaaggctg ggacaaaacc aaagaagccg
     5701 tcaaaaaagg cggcaatgcc gtcggacgcg gcatttccca tctcggcgga aaaatcgaaa
     5761 acgccaccga ataaccgatt cgcgatgccg tctgaaaccg cttcagacgg catcgtcagc
     5821 acaagacgca aacccatgaa agcctttacc aaaattaccg ccatcgtcgc cccgctcgac
     5881 cgcagcaacg tcgataccga cgccatcatc cccaaacaat ttttaaaatc catcaaacgc
     5941 agcggcttcg gccccaatgc ctttgacgaa tggcgttacc tcgaccacgg cgaaccaggc
     6001 atggacaaca gcaaacgccc gttgaaccct gatttctcgc tgaaccagcc gcgttaccaa
     6061 ggcgcgcaaa tcctgttgac gcgtaaaaac ttcggttgcg gctcttcacg cgaacacgcc
     6121 ccttgggcat tggacgacta cggcttccgc gccgtcatcg cccccagctt cgccgacatc
     6181 ttcttcaaca actgctacaa aaacggcctt ttacctatcg tgttgaccga agaacaggtt
     6241 gaccggcttt tcaaagaagt tgaagccaac gaaggctatc agctctccat cgaccttgcc
     6301 gaacaaaccc tgaccacccc tggcggcgaa acattcacat tcgacattac cgaacaccgc
     6361 aaacactgcc tcttaaacgg cttggacgaa atcggactga ccctgcaaca tgccgatgaa
     6421 attcacgcct ttgaagaaaa acgccgccaa agccagcctt ggctgtttaa cggttaaaac
     6481 ctatgccgtc tgaaggccgt tcagacggca ttcctgataa catataattg ttgcggctaa
     6541 tgtaaacaga ggggcgataa attgctttca tcttttaagc gggcttagcg acactctaaa
     6601 ttaatgcaat aatgcaactc tccatttcaa ataaattttt agatttttaa tatgcaacaa
     6661 attaaattta ttgacttatt ttccggaatg agtggcatca ggaaaggatt cgaacaagcc
     6721 tgtcggaaac agtcggttgc ttgcgaatgt gtttttactt cagaaatcaa accggcagct
     6781 ttggaagtat tgaagcaaaa ctaccctgat gaagtgccgt atggagatat aacgaaaatt
     6841 gaaacagggg atattcccga ttttgacatc ctgttggcag gcttcccttg tcaggctttt
     6901 tctttcgccg gaaaaagatt aggctttgaa gatacacggg gaacgctttt ctttgatgtg
     6961 gcaaggattt taaaggcaaa aaaaccaaaa ggttttattt tggaaaatgt ggaaggattg
     7021 gtgacacacg atagaaaaga ttcgacacaa aaaatagggc gcaccctgac cgttattttg
     7081 gaaaccttgg aagcattggg ctactatgtt tcttggaaag ttttaaatgc aaaagatttc
     7141 ggcattcccc aaaacaggaa gcgcatttat ctgacaggca gtttgaaatc caaacccgac
     7201 ttatctttcg aaacaagccc aagtccgaaa ttaaaaaata ttttggaatc gggactgcct
     7261 accgaaagca gccctttcat caaaaaattg ctaaaaaaat ttcccccgtc cgaactgtac
     7321 ggaaaatcag taaaagacaa acggggaggg aaaaacaata ttcacagttg ggatattgaa
     7381 ttaaaaggcg cagtaaccga agaagagaag caattgttaa atatccttct aaaagaacgg
     7441 aggaaaaaaa atgggcttca gaaatcggca tagattggat ggatgggatg cctttgacaa
     7501 aagcgcaaat ttcaactttc tataaacacc ccaatctcca aaatattttg gacagcctga
     7561 cagataaagg ctatttggtt ttagagcatc cgaaacaaaa aattggcggg caaagaatca
     7621 aagatgaatc cctgcccaaa ggctacaata ttgtttcagg taaaaaatct tttgaaatca
     7681 ataaaatatt agatccaaac gatgttgcgc caaccttggt tgcaatggat atggagcacc
     7741 ttttcgtcgt tgacaacggc ggtttgcgta cacttaccgg aaaagaaggg ttgcgcttat
     7801 tcggctatcc ggacgattat tcgtttgata ttcccaaaaa agacagatgc gatttattgg
     7861 gtaataccgt tgtcgtccct gtgattaagg cggtatctga aagacttctg catactttat
     7921 aaaccgacag ggataaaatg ataaaactta ctgcacaaca aatatttgat aagttgctgg
     7981 atgaagaaaa aatcttatcg gccaatggtc aaattagatt tttcttaggc gatgtggata
     8041 ttatcgtcaa acaaaaagat gttgtcggca acatcattca ggaatggctt tgaatgcttg
     8101 gaagattttg tttccgcaat tgaagaaacc gtttatcaaa acccggcaac acggcataac
     8161 gcatccttat ggaaaaagaa atttgaggaa gcctataaaa aacactacaa ccgatcgatt
     8221 tccatacctc gttggcacga aattgcacac aaatacaaaa agaaataacc cttcaaccat
     8281 caacccataa ggaaacttga aatgaccaaa catatcgcca tcctccgagg tgacggcatc
     8341 ggtcccgaaa tcgtcgccga aaccgtccgc gtactcgaca aactcatcgc ccaaggtttg
     8401 gatgccggct acgaatacgc cccattgggc ggcgaagcct atgacgaata cggtcatcct
     8461 tatcccgaat ttacgcaaaa cctctgccgc aaagccgatg ccgtcctgct tggtgcagtc
     8521 ggttctcctc aatacgacaa cctcgaccgt ccgttgcgcc ctgagcgcgg cctgttggca
     8581 atccgtaagg atttgaacct gtttgccaat ttgcgtcctg ctgttttata tcccgaatta
     8641 gccaacgcct ccacgctgaa gcctgaaatc gttgccggtt tggatatttt gattgtgcgc
     8701 gaactcaccg gcgacatcta tttcggcgaa ccgcgcggca tccgcgttct ggaaaacggc
     8761 gaacgcgaag gctacaacac catgaaatac agcgaaagcg aaatccgccg tattgcccat
     8821 gttgccttcc aatccgccca aaaacgcagc aaaaaagtct gttccgtagg caaagccaac
     8881 gttttggaaa ccaccgaatt gtggcgcgaa atctttgaag aaatcggcaa agagtaccct
     8941 gatgtcgagc tttcccatat gtacgtcgac aacgccgcca tgcagctcgt gcgtgcgccc
     9001 aaacaattcg acgtcattgc caccggcaac atcttcggcg acattctctc cgacgaagcc
     9061 tccatgctga ccggctccat cggtatgctg ccttccgcct cgctggacga aaacggcaaa
     9121 ggcctgtacg aaccgtctca cggctccgcc cccgacatcg ccggacaaaa caaagccaac
     9181 ccgctggcca ccatcctctc gctcgcaatg ttgctgcgtt acagcctgaa cgacgaagcg
     9241 cgcgcgcaac aagttgaaaa cgccgtccaa aaagtgctgc aacaaggctt gcgtaccagc
     9301 gacatttacg aagaaggcac aaaactcgtt tcctgctccg aaatgggcga tgcgatactc
     9361 gccgccttgt aaaaaaacaa agcgcggatc acaccgcgct ttttcatccg cccttttccg
     9421 cccgccccgt tcgtcaaccg cctgcaaacc caccgtttac cggcaggaaa tgccataaat
     9481 ccctgcctat gttaggataa acacctttat cggcccacac aaaggattat catcatgaaa
     9541 aaaatcatct tcgccgcact cgcagcggca gccgttggca ctgcctccgc cgccacctac
     9601 aaagtggacg aatatcacgc caacgcccgt ttcgccatcg accatttcaa caccagcacc
     9661 aacgtcggcg gtttttacgg tctgaccggt tccgtcgagt tcgaccaagc aaaacgcgac
     9721 ggtaaaatcg acatcaccat ccccgttgcc aacctgcaaa gcggttcgca acactttacc
     9781 gaccacctga aatcagccga catcttcgat gccgcccaat atccggacat ccgctttgtt
     9841 tccaccaaat tcaacttcaa cggcaaaaaa ctggtttccg ttgacggcaa cctgaccatg
     9901 cacggcaaaa ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta ccaaagcccg
     9961 atggcgaaaa ccgaagtttg cggcggcgac ttcagcacca ccatcgaccg caccaaatgg
    10021 ggcgtggact acctcgttaa cgttggtatg accaaaagcg tccgcatcga catccaaatc
    10081 gaggcagcca aacaataaat cttgatgcga atcagcacaa atgccgtctt catcaaaccg
    10141 ccccgccaac ggtaagccga tatggcggag cacaatccgc cgaaagatat ttcagcggca
    10201 ggcagggctt gccggaaagt ccggaaa
//
LOCUS       DAWHXS010000068        10137 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWHXS010000068 DAWHXS010000000
VERSION     DAWHXS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.068"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..302)
                     /locus_tag="WIE22_001406"
     CDS             complement(<1..302)
                     /locus_tag="WIE22_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ4127478.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQG"
     gene            complement(688..963)
                     /locus_tag="WIE22_001407"
     CDS             complement(688..963)
                     /locus_tag="WIE22_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ4127479.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(960..2243)
                     /locus_tag="WIE22_001408"
     CDS             complement(960..2243)
                     /locus_tag="WIE22_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4127480.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2399..3817)
                     /gene="glnA"
                     /locus_tag="WIE22_001409"
     CDS             complement(2399..3817)
                     /gene="glnA"
                     /locus_tag="WIE22_001409"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ4127481.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3901..4050
                     /locus_tag="WIE22_001410"
     CDS             3901..4050
                     /locus_tag="WIE22_001410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127482.1"
                     /translation="MPSETRPASNFKLENIVSDGKIVNNMLRFVCSKAEYPPFCPNAD
                     KTDAA"
     gene            4128..4937
                     /gene="aroE"
                     /locus_tag="WIE22_001411"
     CDS             4128..4937
                     /gene="aroE"
                     /locus_tag="WIE22_001411"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ4127483.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSREIFRNCRLAYDMVYGDA
                     AQAFLNFAQSNGAAEVSDGLGMLVGQAAASYHIWRGFTPDIRPIIEYMKAL"
     gene            4940..5641
                     /gene="mtgA"
                     /locus_tag="WIE22_001412"
     CDS             4940..5641
                     /gene="mtgA"
                     /locus_tag="WIE22_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ4127484.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTACVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(5966..6041)
                     /locus_tag="WIE22_001413"
     tRNA            complement(5966..6041)
                     /locus_tag="WIE22_001413"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6006..6008),aa:Phe,seq:gaa)
     gene            complement(6232..6966)
                     /gene="lptB"
                     /locus_tag="WIE22_001414"
     CDS             complement(6232..6966)
                     /gene="lptB"
                     /locus_tag="WIE22_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ4127485.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7040..7570)
                     /gene="lptA"
                     /locus_tag="WIE22_001415"
     CDS             complement(7040..7570)
                     /gene="lptA"
                     /locus_tag="WIE22_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ4127486.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7551..8132)
                     /gene="lptC"
                     /locus_tag="WIE22_001416"
     CDS             complement(7551..8132)
                     /gene="lptC"
                     /locus_tag="WIE22_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ4127487.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8129..8665)
                     /locus_tag="WIE22_001417"
     CDS             complement(8129..8665)
                     /locus_tag="WIE22_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4127488.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKILQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8883..9857)
                     /locus_tag="WIE22_001418"
     CDS             complement(8883..9857)
                     /locus_tag="WIE22_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ4127489.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9954..>10137
                     /locus_tag="WIE22_001419"
     CDS             9954..>10137
                     /locus_tag="WIE22_001419"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="catalyzes the reversible formation of D-erythrose
                     4-phosphate and D-fructose 6-phosphate from sedoheptulose
                     7-phosphate and D-glyceraldehyde 3-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ4127490.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEV"
BASE COUNT         2378 a         2733 c         2627 g         2399 t
ORIGIN      
        1 ccttggtcgg tttttttgta atagacgcta atctctatca aattaatgga gtaagcatcc
       61 tgcccgggcg tgtcggaaag ccattttgtg gcattgtctt gcgtataccg tgcaagatag
      121 ccataaggga taaccatttt ggtgccctga aaatcaaatg taaccgtatg ttcaaatcct
      181 gtcattggct cgggattgtt gaaactggtt tgttcattaa caggttcaca tgaggtcata
      241 gtaaaaacac tcccaataaa caaaataaaa agttgataaa tggtagtagc atatggtctc
      301 ataatttcaa gcctagaaat tagttaaaga atatattatg gtagatacta actctttaag
      361 ttgacatatt gtgcgatgtc gagggctgta ccatattttc ccatcgtatt ttcccgttct
      421 ttatatgagc ggcaggcatc ctcataaaag aagggatacc gatacggaaa aataccccgg
      481 cataaaaaat atactgatta atcaatacaa atccaacaaa cagttcatcg tattcttttc
      541 tgttggcgta taaaaaatac tgatatatgc cttcactttc atagggcgac gctttatgta
      601 ccgtcctgtg tgttgaaacg tcggttttcc ttaccgcctg ccccgctttt ccctgattgc
      661 tgccaactgc cgtccgaacc tgaaccgtca ggtttcagac ggcatttttt ccgcctgttc
      721 ccacaatttc cgtttttgtg cggcggtcag ttttttgacg gcgatttcct gtttgagcgc
      781 cgtgccttta tccatcccgc ctgcaacgat acgcatcgcc accggtttga atacgcgggt
      841 atatttcgcg cctttaccgg ttgtgtgggc ggcaagccgc tgttgcggat tcgggctgat
      901 gccgcaatag aacgcgctgt tttcacacaa tatcaggtaa acgctccagt ttgacgcgtt
      961 catctgcctg catcctgagt tttttccccg ttccaaggcg caactttcag cagcagcagc
     1021 atacccggca gggcaagtat gaaacacagc tggaaaaacg gtacataacc gagccattcg
     1081 atcagataac cggcaaagga attgatgacc gtgcgcggga cggcggacag gctggtaaac
     1141 agcgcaagct gcgttgcggt aaatgcggga ttggtttcac gcgccatata cgatacgaac
     1201 gccgccgtcc ccaaccccac gccgaccgct tccgcgccga taactgccgc cagcatcagc
     1261 ctctcgcctg tgccgaccgt gtcgaaaggt ccgaaccctg ccagccatac aaaccccaaa
     1321 acggttacag cctgcaccgc gccgaatagc cacaaggctt tgtttacgcc gattttcagc
     1381 atccacacgc cgcccaagat acctgccgcc actgccggcc acagtcctgc attttttgca
     1441 atcaaaccga tgtctgtctt actgaaaccc atatccagat aaaacggcgt tgccaacgcg
     1501 gttgccatac tgtcgccgag tttgtaaagg aagataaaca gcagcacgca caccgccgaa
     1561 gcgatgccct tgcgcgtaaa aaattcttta aacggctcta ccacggtctg cttcaacgtt
     1621 ttaggaacgg caggcggcaa cacgggttcg cgcgcaagaa acagcgtcat cagaagaccg
     1681 ggcagcataa acagcgaagt gatgacaaac acctccggcc acggcatcct gtctgccagc
     1741 accaaactca atgaaccggg aatcagggcg gcaacccgat aggcgttcac atgaaccgag
     1801 ttgcccaaac ccaattcctc atccgacaaa atctcgcgcc tgaacgcatc caatacaatg
     1861 tcctgactgg cggaaaaaaa agcgacaagc accgacaagc cggcaatcag cggcagatga
     1921 ttacgagggt ttaaaaaggc ataagccgcc aaagccgcca gcaaacctgc ctgcgtcagc
     1981 agcatccacc cgcgccgccg ccccaaaacg ggcagcctga ccgcgtccat cagcggcgac
     2041 cacaaaaatt tccaagtaaa cggcagaccg attaacgcca tcagcccgat gctcttcaaa
     2101 tccacctgct cgctgcgtaa ccacgccgga atcaggttaa tcagaaagta cagcggcagc
     2161 cccgaggcaa accccgtaaa aatacagaca agcatattgc gggaaaagat ttgcccgata
     2221 aaacctgatt tcgatgcagt catcgttcgt gcagcccaaa aaaaacaaaa gcctattgta
     2281 gcaaatatcg cccgcctgct gaaaaacgcc gccccgcgcc gccggtttgc cgttcaaatg
     2341 caaaatgccg tctgaaactg tgttcagacg gcattgcttt tctgaaaacc agacgtgctt
     2401 acaggctgta atacatttca aattccagcg ggtgcggcgc catgcggatg cggcgtacgt
     2461 cttcttcttt gaaagcgatg tagctgtcga tccagtcttt gctgaacacg ccgccgcgca
     2521 ggaggaattc gtggtcggct ttgagggcgg ccagtgcttc ttccaaagaa gcgcaaacgg
     2581 tcggcaccaa tgcatcttct tccggcggca gatcgtacag gtttttatcg gcagggtcgc
     2641 ccggatggat tttgttttga atgccgtcca aacccgccat caacagggcg gcaaatgcca
     2701 aatacgggtt ggcggtcgga tcggggaaac gcgcttcgat gcggcgcgcc ttgctgctgt
     2761 tcacggacgg aatgcggatg gaagcggaac ggtttttggc ggagtatgcc agtttggtcg
     2821 gcgcttcaaa gtgcggcacg aggcgtttgt aggagttggt ggacggattg gtaatcgcgt
     2881 tcaaggcttt ggcgtgtttg atgatgccgc cgatgtagta gagcgcggtg tcgctcaagc
     2941 cggcatagcc gtcgcctgcg aacaggtttt gaccgtcttt ccaaatggat tggtgaacgt
     3001 gcataccgct gccgttgtcg cccataatgg gtttgggcat gaaagtggcg gttttgccga
     3061 agttgtgggc aacgttttga atcacatatt tcatgtcttg ggtttggtcg gcgcgtttga
     3121 ccaaagtagc aaagcgcgtg ccgatttcca tttggctgcc ggtgccgact tcgctgtggt
     3181 gcacttccac ttcaataccg agttcttcca aaatgtttac catcgccgaa cgcaaatcct
     3241 gaccgcagtc aatcggtgca acaggtgcgt aaccgccttt gacggtcggg cggtggccgg
     3301 tgttttgacc gtccatatgc agaccgcttg cccacgcgcc gctttcggac gtgatttcgt
     3361 aacgggtttt gtgcatatcg gtttcaaatt ctatgccgtc gaatacgaaa aactcgggtt
     3421 cgggaccgaa ataggcggtc tcgccgatac cggaagattt caaataggct tcggctcggc
     3481 gggcgatgga gcgcgggtcg cggtcgtaac cctgaccgtc ggcgggatcg ataacgtcgc
     3541 aagtcaacac aacagtcgca tcatcataaa aaggatcgac gaaggctgta gacgcatcgg
     3601 gacgcaactg catatcggaa gcctgaatgc ctttccagcc gccgatagac gaaccgtcaa
     3661 acgcctgacc gttttcgaac cactcttcgg ggtcttccaa cacgatgcgc gcaggcacgg
     3721 taaagtggtg ctgcttgcct ttggtatcgg taaagcgcaa atcgacaaag cgggcttcgc
     3781 tttcttcaat caattttacg gcgtttttaa tagacatttt tcagctcctg gaaaagggaa
     3841 atagcaaaat atacggaacg cggcagatta gcccaaagcc agcatgattc taccacgtcc
     3901 atgccgtctg aaactcgccc tgcgtcaaat ttcaagctgg aaaatatagt ttcagacggc
     3961 aagattgtca acaatatgct tcggtttgtc tgttcaaaag cagaataccc gcccttttgt
     4021 ccgaatgccg acaaaacaga tgccgcctga aaacagttta gacggcatcg ttcccatctt
     4081 cgcgccgccg ttataaaatg accgccccgc cttgatggaa tatgactatg cacgccctcc
     4141 cccgctacgc cgtttttggc aaccccgtcg cccacagcaa gtcgccgcag attcatcaac
     4201 aatttgccct tcaggaaggc gttgacattg aatacgaacg catttgcgcc gacatcggcg
     4261 gtttcgcgca ggcggtttcg acattttttg aaacaggcgg ttgcggggca aacgttaccg
     4321 taccgttcaa gcaggaagcg tttcatctgg cggacgagca ttctgaacgc gcattggctg
     4381 ccggcgcggt caacacgctg atttttctga aaaacggaaa actgcgcggc gacaataccg
     4441 acggtatcgg tttggccaac gacatcacgc aggtcaaaaa cattgccatc gaaggcaaaa
     4501 ccatcttgct tttgggcgcg ggcggcgcgg tgcgcggcgt gattcctgtt ttgaaagaac
     4561 accgtcctgc ccgtatcgtc attgccaacc gcacccacgc caaagccgaa gaattggcgc
     4621 ggcttttcgg cattgaagcc gtcccgatgg cggatgtgaa cggcggtttt gatatcatca
     4681 tcaacggcac gtccggcggc ttgagcggtc agcttcctgc cgtcagccgt gaaatttttc
     4741 gcaactgccg ccttgcctac gatatggttt acggcgacgc ggcgcaggcg tttttgaact
     4801 ttgcccaaag caacggcgcg gccgaagttt cagacggcct gggcatgctg gtcggtcagg
     4861 ctgccgcctc ctaccatatt tggcgcgggt ttacgcctga tatccgcccc attatcgaat
     4921 acatgaaagc cctgtaacca tgttccgcat catcaaatgg ctgattgccc tgcccgtcgg
     4981 catctttatc tttttcaatg cctatgtgta cggcaacatc attacctacc gcgccgtcgc
     5041 gccccatcag actgccttta tgtcgatgcg gatgaagcag tttgaacagg aaggtcgcga
     5101 tgtcgcactg gattaccgct ggatgcccta caaccgcatc tcggtcaacc tgaaaaaagc
     5161 cctgattgct tccgaagatg cccgtttcgc cgggcacggc ggcttcgatt ggggcggcat
     5221 tcaaaacgcc atcaggcgca accggaacag cggcaaagtg aaggcgggcg gctcgaccat
     5281 cagccagcag cttgccaaaa acctcttcct caacgaaagc cgcagctata tccgcaaagg
     5341 cgaagaagcg gcgattaccg cgatgatgga agccgttacc gacaaagaca ggatttttga
     5401 actgtattta aactcaatcg aatggcacta cggcgttttc ggcgcggaag ccgcgtcccg
     5461 gtatttttat caaatacccg ccgccaagct gaccaaacag caggcggcaa aactgacggc
     5521 gtgcgtcccc gccccgctct actacgccga ccatccgaaa agcaaacggc tccgcaacaa
     5581 aaccaatatc gtgctcagac gcatgggttc ggcagagttg cctgaaagcg acacggactg
     5641 attgtttcag atatggaaat gccgtctgaa ctgcggttcg gacggcatat gttttctggt
     5701 ttttatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     5761 cacggaatcg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
     5821 cgagccaacg ctgtaccggt ttaaatttaa tccactatac ctgcccgaca gccgcaaaga
     5881 ccgtatcata catagcaggc attgcggtgc gggattactg ccgcaaacgg caggcaaaaa
     5941 aaataaccgc ttcaaggcgg ttttttggtg gccagaggcg gaatcgaacc gccgacacac
     6001 ggattttcaa tccgttgctc taccaactga gctatctggc ctctgtctgt ctcgttaaga
     6061 gatgcgtatt aaaccaaatt acgggacgac atgcaagcat atcgggcaat attctttgaa
     6121 aacaggttta agtcaatgtt ttaaatttga attaatttat gtaaaaacaa cataaatccg
     6181 tatttttgcc gcctgcccga cgatattagg tcgcccgtct gaaaaatatt ttcaatattt
     6241 gaagttctta cccagataaa cagaacgaac ctgttcgttt ccgaccaaat catcaggttt
     6301 tcccgatgcc agcaccgcac cgtctgaaat gatgtaggcg cggtcgcaga tgctgagggt
     6361 ttcgcgtacg ttgtggtcgg taatcagtac gccgataccg cgcgacttga gaaatccgat
     6421 gattttttgg atgtcgatga cggcgatggg atcaacaccg gcaaaaggct catccagcaa
     6481 aataaaatgc ggtttcatgg cgagtacgcg ggcgatttcg acgcgccgcc gttcgccgcc
     6541 ggacagcgac ggcgcggggc tgcggcgcaa gtgtccgata ttaaggtcgg cgagcagttt
     6601 ttcgatttcc ctgtcgattt gatttttatc tttggttctg atttccaaga tggcgcggat
     6661 gttttgttcg acggtcattt tgcggaatat cgaggcttcc tgcggcaggt agccgacacc
     6721 gaggcgggcg cgttcgtgta tgggcaggtg gcgcaattct tgtccgtcta gggttacgct
     6781 gcctgcgtcg gcggcaatga gtccgactat catgtagaag ctggtggttt tacccgcgcc
     6841 gttgggcccg agcaggccga cgacttctcc gctttcgatt tcgagggaga agcttttaac
     6901 gacttggcgt tttttgaaac ttttttgcag gttttgaaca acaaggcggc tgacgtttgc
     6961 gctcataaat atctcttcaa tatcttcggt cggcaaatgc cgtccgaacc aggtttatgt
     7021 ttcagacggc attttgggat tattcggatt tttgcgtact cgaaggctgg ataacgacgc
     7081 tgaccctgcc ggatttggaa gcggattttg cgccggattt tgtgctgccg ctgatggtat
     7141 agacttcggt tttggtgttg tatgtaatca ccgcaccttc ggcgacatcg ccgccgcgct
     7201 gtactttggc attaccggtt aagactacgg tgctgcctgc agatgaataa gcaacgttgt
     7261 ttgcctgtcc gcgcaccgtg cctttgccgc cgtccaatgt ctggctgaag cggactggcg
     7321 aaccttccgc cctcacggat tcgccgcctt tgccgccgcg tgtaacattg acgcgggcgg
     7381 cggaaatatt gagcgtaccc tgtctgatga cgacgtttcc gctgaatgtg gtgctttggt
     7441 tggcttgatc gagcgaacct tggtcggcct caatctgaat aggctgcctg ctgtcgcttt
     7501 gaagggcaaa agcgggggac gccgaaaaaa atgctattaa aacaaatagc ttacatatct
     7561 tttgtatcat aaatcgtggc tttcacttta gatgagaagt tcaacatgcc tgttttgtgg
     7621 tcgtaagtca tgccgcccgc ctgaccgtgc gatgcaccat attggaaact gacaggcgta
     7681 tcggtttggg catattgaga ttcggtatcg acgtgcagct tttcggcttc aactttaccc
     7741 gcctgccgtt tgccgtcggc ggttttggtc agcacaacgt tgtttttaaa aagaacctgt
     7801 ttgttttcgg tatggtaaac ggcttcatcg ctgccgactt cgtacaacaa cctgccttct
     7861 tggaagaaca cgagatgcgg tgagtcgaaa tgaatgtcgc tgctttcggg aaactgtttc
     7921 gcgcccttcg aactcaaatg ttctttcaag tatccctgtt cgtcaaaccg cctgccatcc
     7981 aatccgtcca ttgtgtattg cggttcgtcg ggattgagcc tgacttcttc aatctcgact
     8041 tcgctgatgc gtcccaacca tgccgacagg ctgcccaagg caaccgccaa tatcaatggg
     8101 aacgcaattc cgtaccgcca tcttactttc atttgatgta ctcgttcaaa gccgcgccca
     8161 aagtcccttg cgcctgcata atcaggtcgc acacttcgcg caccgcgccc gcaccgccgg
     8221 ggcgtttggc aatataggcg gcgtgttgca gcgtaaacca atgcgcgtcg gggacggcaa
     8281 ccggcaatcc gcagcgtacc ataacgggca aatcgaccac gtcgtcgccg acaaaggcac
     8341 actcggcttc ttccacgccg acctgagcgc gcagctcttc atacgcggcg cgtttgtccg
     8401 aaatgccttt gaagtagtaa tttatgccca actgtttgac gcggatgccg acggagggcg
     8461 cgtcccgacc tgtgataatc gcagtctgca cgccgcttgc ctgaagtatt ttcagaccgt
     8521 gtccgtccag tgtgtgaaac gatttgattt cttcgccgtt atcgcggata aagatgcgcc
     8581 cgtcggtcaa aacgccgtcc acatccagga tcaacagttt gattttggcg gcgcgcgcct
     8641 gtaattcggg agaaattgcc tgcatgggat ttccttgttt tcagacggca tatcttacca
     8701 caaatgccgt ctgaaacggc tgcaacgcct tatatagtgg attaacaaaa atcaggacaa
     8761 ggcgacgaag ccgcagacag tacagatagt acggaaccga ttcacttggt gcttcagcac
     8821 cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtt tttgttaatc
     8881 cactatacaa tccgcgccgc caaaaggtcg tgcatattca gcgcgccgat cagcacgcca
     8941 tctgcatcgg taaccagaag cccgttcaca tggtttgcct gcatgacttt cagggcttcg
     9001 gtggcgagac gttcggcgga gatggtttta ggatgcgtat gcatgacttc gtctatcgaa
     9061 agaccggtaa aattgtcgca ttcttgaaac aggcggcgca aatcgccgtc ggtgaatacg
     9121 cctttcagac ggccttgccc gtccgttacc gccaacatgc ccagcccttt ttcactcatg
     9181 ctgacgatgg cttctttcaa gggcgtgccg agtcggacgg caggcaggcc gccgcctttg
     9241 tgcataatgt cggcaacgcg caaaagtagg cgtttgccga ggctgccggc agggtggctc
     9301 aaggcgaagt cgtcgggcgt gaacgcgcgg gcgcgcagca ggacaaccgc caacgcatcg
     9361 cccaaagcca taacggcggt ggtgctggtg gtcggggcaa gccccagcgg gcaggcttct
     9421 ttggaaaccg acgccgtgat gtggatgtcg gcatggcgcg ccatggttga atcggggcgg
     9481 gcggtgatgc agacaagcgt gatatctttg cgtttgagcg cggggatgat ggcggcgatt
     9541 tcgtcgcttt caccggaatt ggaaatcgcg acgaccacgt cgttgtccac aatcatgccc
     9601 aaatcgccgt gtgccgcttc cgcagggtgg acgaaaaacg cgggcgtgcc ggtcgaggcc
     9661 atggttgccg ccattttgcg cccgatatgt cccgacttgc ccatgcccgt gataacgacc
     9721 ctgcctttgc agtgcaacaa cgcgtccgcc gcaagggcga agttttcgtc caaatccgcc
     9781 gcaatttcgc gcaagccttc cgcttcggtg tgcaacactt cgcgtgccca atcaagatat
     9841 ttttcgtttc ctgccatcgc catccgcctt tgctgtaata aaactaccca aaatttccga
     9901 atcggggtag cataagcctt tgaaacatac acatccatcc aaaggaatca gaaatgacta
     9961 ttttatcgga cgttaaagca ttaggacaac aaatctggct ggacaacctt tcccgctcgc
    10021 tcgtgcaaag cggcgaattg gcgcaaatgc tgaaacaagg cgtgtgcggc gtaacttcca
    10081 atcccgccat ctttcaaaaa gccttcgccg gcgatgcgct ttacgccgac gaggttg
//
LOCUS       DAWHXS010000069         9898 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWHXS010000069 DAWHXS010000000
VERSION     DAWHXS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.069"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(305..4474)
                     /locus_tag="WIE22_001420"
     CDS             complement(305..4474)
                     /locus_tag="WIE22_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ4127491.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTESGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     AVYLDRFETGKISVGKTFDKQTVYLERLDASYRYDIKGHRLDLKTADTPWSKSTGAVV
                     IGLKKPFVLNTAIYTKGELEGETIHGTARLWGSLQDMHTDLILDGDNIHLSGKSVIHP
                     FAESLDKTLEEVLIKGFNINPSAFLPSLPKARLNFDLTAIPSFSDGIALEGSLDLENT
                     EAGFADRNSIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPTGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETNLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADLKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTRKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4529..6358)
                     /locus_tag="WIE22_001421"
     CDS             complement(4529..6358)
                     /locus_tag="WIE22_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ4127492.1"
                     /translation="MMIKPTALLLPALFFFPHAYAPAADLSENKSAGFALFKNKSPDT
                     ESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTM
                     LRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALE
                     NWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADLNVVVDS
                     GRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQ
                     ADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSVV
                     WDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVRDRAGID
                     ARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTL
                     GTFLSSTALIRASARAGYFFTPENKKLGTFIIRGQAGYTIARDNADVPSGLMFRSGGA
                     SSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANFKR
                     MELKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6904..8133
                     /gene="sstT"
                     /locus_tag="WIE22_001422"
     CDS             6904..8133
                     /gene="sstT"
                     /locus_tag="WIE22_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ4127493.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8245..9525
                     /locus_tag="WIE22_001423"
     CDS             8245..9525
                     /locus_tag="WIE22_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4127494.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNAGNTAAQGTNQAENNQVGGSQNPAPSSNPNATNGGNFGRVDLANGVLIDGPSQNI
                     TLTHCKSDSCNGDNLLSEEAPSKSEFEQLSDEDKIKKYKKDGEKFTGLVADRLQMKGT
                     NQYIIFYKPKTTSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHTENGRSYP
                     TKGRFAAKVDFGSKSVDGIIDSGDDLHMGKQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9602..9817)
                     /locus_tag="WIE22_001424"
     CDS             complement(9602..9817)
                     /locus_tag="WIE22_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127495.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9810..>9898)
                     /locus_tag="WIE22_001425"
     CDS             complement(9810..>9898)
                     /locus_tag="WIE22_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ4127496.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT         2006 a         2505 c         3039 g         2348 t
ORIGIN      
        1 tcgggcattc cttaaattac ctgtgtatcg ctgtaaatct tagagatggc ggaatatagc
       61 ggattaacaa aaaccggtac ggcgttgcct cgccttggct caaagagaac gattctctaa
      121 ggtgctcaag caccgagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc
      181 cttatcctga tttttgttaa ttcactatat cgattctgtt cccttgcagg cggacggcgg
      241 cggcggacgg tggaaaagca ggttttcaaa tgtccggttt ggcggcgcgc cgtctgaaaa
      301 ccgcttattt tcctttgccg tttccggcgg agtctttttt gtccgaaccg gagaagcggt
      361 cgaaacgtat ggtgtatgtc agctcgccgc ccgacgaacg gctgccgata cgggcaaccg
      421 cctgtatggc gcgggtcagc cggtaaatca gtttgacgga ctgttccgcg ctggagatgc
      481 tgtattcgta gccgatgtag agtttgccgg tcagttgttt gccgacggtc agcacctgtt
      541 cggcggggtt gagttcgccg gtttgcgcgt tgcggctgcg cttgcgggta aagcccaaat
      601 catccaccag cccgatgcgg tcgttgattt gcccggcaag cagcgcgcct gcggctgcgg
      661 acagggcggc attgtcgccg ctgctgccgc tgccggcgcg gttgaggatg agccaagaga
      721 gcttgtcttt ttcactcatc ggttcgtttg ccgtcagcgt aatgcgcggg ctgttgaggc
      781 tgcccaatat ttccacgccc gcaccgacgg gggaaaggcg gcgttcggcg cggatgttga
      841 ggttggggtc gttgagcggg ccgacaaagg agaccgtgcc tttggtaatg tccaaatcct
      901 gcccgtatgc cttataacgc cctttgatga cgcggaccgt gcccacgccc cgcacgcttc
      961 cgcccgattg ggcggtcagg gtcagtttgc cgcctatggt aacgtccgcg ccgtagccgg
     1021 cgaagcggat gccgtcattg aggtctaaag tcaggttcat attgacgggg agcggtgccg
     1081 ccgcctcttt tttgacttcg cctaatacga cgacatcgtc gccgacggac ggcatcgagg
     1141 atttttgcga accgaacagc ccctgatccg ttttaatcat tccggtaacg gatatgcctt
     1201 tttgcggcga atagcgcagg cgggtgttgc cggaaaccgt caggcggcgg ttggggcggg
     1261 acaggatgcg gtatttgtcg aacaccgcgc cgatatcgac atcgggtccg ctgttttcca
     1321 taccgaccgt accggagagt tccgccgtcc cttcgtgccg gaatttcagg ctgtcgatta
     1381 cccatttcct gcccgcgata tgcgaacgca gcgagccgtt gtccaagatg atgccttggg
     1441 tttggttgcg gtaatagagt ttgtcgccgt tgatgctgcc gcccaagtgc ggatcggcga
     1501 tgctgccgcc gagggttacg gcggcattca ggctgccttt gacggtttgt ccgaccggta
     1561 ggaagttgcg gaatacttcg gcatcggcaa cggtcaggtt gagcctgccg cccaaaggcg
     1621 cggtatcgaa agagcggctt tgcccgacgg tgatgttgcc gttgattttc ccgtagttgc
     1681 tgctgccgtt gacggcggca ttgacggacg gagagcctac ccgtccgccg atttgcgcgg
     1741 cggcattcag gctgccggta atgttttgcg cggcggcggg cagaaagggc ttcaatgcgc
     1801 ccaagtcggg aagggaggcg gtaatcctgc cgccgagcgg tgcatttgcc atattgccgc
     1861 cgaaggcgtt ggcgatgccc aaatcggcgt taatccgccc gaaacgcgcg ccgccgtcaa
     1921 gcaggattcc gatacggtcg ttttgaaagc gcgttttcag ggaaaatgcg ttcaaaccca
     1981 aagcctgccc gccgggcaat acggcatcgc cgctttgccg gctgatattg aggtagccgc
     2041 gcgcgttgcg cccgtaggcg acatcccagt cgccgtttaa aaccagattg tgttcgaagg
     2101 gcggtttgaa gaaattgtgc aactcggcga tatgcagacc gtgtgcgccg ccttttgccg
     2161 atatgccggt ttttttatcc caagaaaagt gttgcaggtt gaggctgccg cccattgcct
     2221 gccaatttgc cgcacttgcc gccacgcgtt ccgcaccggc ttcgagcgtc atacggtttt
     2281 gcagcttgag gttgaatgcg ccgccgatgt cgaggatgcc gatgctgcct ttccatcggg
     2341 taagttccct gttgatgccg cctgaagcgt ccaaatcgaa tttgaacggt ttgccatcca
     2401 gcgtcatggc ggcgtgtgtg cggatgcggt gctgcacgcc cgtgccgtcc agcatcaggt
     2461 cggcggtatc gacaaccgcc gctccgcccg acagcgaaag gcggctgcct ttgaggtcgg
     2521 cgcgtatcgg gcggcttgtg tcgggcgaac ctttgagcgt gaaatcgagc gaacggatgt
     2581 ctgccgcctt gccgatgtgc aggttgcgcg ccgcgccgga aaggttggtt tcaaaggttc
     2641 ggatgccgcc gtccaaatcg ccggaaaggt gtccgcgtac atttaaagac cccgcgagtc
     2701 cgaaaccgaa acgggataaa tcgggtgcgg tgatattgag gttaagccgg tcgccttttt
     2761 tgccgaagcc gccgtctgtt ttgatgatgt tccgccccag ccgcaaatcg acggcggcac
     2821 gcggaaggtg gcgggactcg taaacaatgt cggcactgcc ggcaatcggt acgccgttga
     2881 acgtgccggg taaaaaccgc attttgcctg tgaatttctc ttttgccagt tcgccggcaa
     2941 ggtttattga gccgttgata ttgcctgtcg gaagttgcgg atcgatgcgc gaagggtcga
     3001 atgcgcggga acggatgtcc agcttgagca ggcggtcttt aaacagctcg agatagcctt
     3061 gcgcggtcag gctgccttgc ccggcggcga tgttgacggt gtcgagcacc agtttccgct
     3121 gtccgtttgc ggggtcgctt gcaatagcga ggctgccgtc cgtgcgcgcc gtgccgatgc
     3181 cgagttgcca agagattttg ggcgaggcgg tcgtaccgcc gatgccgatg ctgccgtcca
     3241 acctgccttt gaacgcggtt tgcagtacgt cttccgcgcc gacggagttg atgcctatat
     3301 ttaaatcgag gatgtctttt tcggtgtcga ttttgcccga cagcctgatg ccgccccgtc
     3361 cgagcagggc ggcggacgta ttgccgatat gcaccgtgcc gtcctgccgg atgacaaagc
     3421 cgcccaaaac ctgacggacg gggatgctgt tgcggtcggc aaaaccggcc tcggtatttt
     3481 ccaggtcgag agaaccttcc agtgcgatgc cgtctgaaaa agacggaatg gcggtcaggt
     3541 caaagttaag ccttgctttg ggtagggagg gcaggaaggc ggatggattg atgttgaatc
     3601 ctttaatcag cacttcttcc aatgttttat ccaatgattc ggcaaacggg tggatgacgg
     3661 atttccccga taaatggata ttgtcgccat ccagtatcag gtcggtgtgc atatcctgca
     3721 aactgcccca caggcgcgcc gtaccatgta tggtttcgcc ttccagttcg cctttggtgt
     3781 agatggcggt gttgaggaca aaaggttttt tcagaccgat tacgactgcg ccggtcgatt
     3841 tgctccacgg ggtgtcggcg gttttcagat cgaggcggtg tcctttgatg tcataacggt
     3901 atgaagcatc cagccgttcg agatagacgg tttgtttgtc aaaagttttg ccgacgctga
     3961 ttttgcccgt ctcgaagcgg tcgagataga cggcggcggg caggtctatg ctgtcgggca
     4021 gaccttgagg cgggcgttct tctttaggcg gagtcggttt ggtaacgatg gcgatgtcgc
     4081 cggcggaaat ttcggtaatg tgcaggctgc ggcgcatcag ttcggacggt ttccacgcga
     4141 agcggaagcg gctgatttta aggtctgctc cctcggtttc tatcgaccag ttgtcgccgt
     4201 cgaagccgtc gagcagcgtg cctttgaggt tttgggagga aatgtttacg ccgaaccaag
     4261 acgggatttg gtacagcccg aagcgcaaac cggattccgt accggcgagc cagccgagga
     4321 aacatactgc caaaatcagg acagacagca gtgccgccga cagcttcagc agcgggcggc
     4381 gtttttttgc cggcggggca gggcggtgtt cagacggcat tttgcgcgtg ccgttttcgg
     4441 tcggatcggt atctgtcggt gcggtatcgg tcataatgtc tgtttcaaaa tagtttgctg
     4501 caatgccgtc tgaagcggcc gtatcggttt agaaacgcgt tcccaggctg atgtgccagc
     4561 ggattttctt gtcgctgtgc ccgtaggcga tgtcgaagga aaacggcgcg agcgggctga
     4621 accagcgtac gcccagtccc gaaccgtgtt tcagctccat acgtttgaaa ttggcggcgg
     4681 catcgcccat atcgtggaac accgcgccgg acagggtgcg cgtaaacggc agttggtatt
     4741 ccaagctgcc caccaagagg gcgcgttcgg gcaggaccga tccgttcggg ccggcaagtc
     4801 cgatgctgtc gagttcgtaa ccgcgcacgg aagacgcgcc gccgctgcgg aacatcagcc
     4861 ccgaggggac atcggcatta tcgcgggcaa tggtgtaacc cgcttgtccg cgtatgatga
     4921 atgtgccgag ttttttgttt tcgggcgtga agaaataacc tgcgcgggcg gaagcgcgga
     4981 tcagcgcggt ggaggacagg aatgtgccca aagtcgtccc gattttgccg tccaggtaat
     5041 ggccgttttc gggatgcagc acgttgttga gcagctggcg tttccaagag gcggtcagca
     5101 tcgtggcgtg gctgttgccc aaatcgacat ccgagccggg gattttccgg ccttctgcga
     5161 gaaactccgc ccccagcctg gcatcgatgc ccgcgcggtc gcgcacatac cagatgccgc
     5221 cggagaaggc gcgtttttcg aggttttggg tggtcgaacg gttgtaggaa acgttgcttg
     5281 tccagtagtt gccccgatag ttgcggggtt ggctgatgcc ggcggcaagc gtggtttcat
     5341 atttgtccat atcccagacg accgagccga tatagccttt gttgaagagg ttgtaatagt
     5401 cgtaggcgat tttgccgccc aaaccgtatt ccgaatcgag gcggatgccg gtttcgagtt
     5461 tgtggcgttt gacctcggtt acgctgactt tgacggggac gcggtcgcct tggagacggt
     5521 cgaagtcggc ttgtacggac gcgccggaat aatgcccgtt ttgttcgagc gcctgttgga
     5581 agtcgagcag caggtcgagg tcgtagggcg tgcccggttg gaagcgcgcc aggccggaga
     5641 cgatttgttc gggataacgc tgtgtgccgg taatttcaaa gtcgccgaag gcgatggggc
     5701 ggccgctgtc cacgacgacg ttcaaatcgg cggtggcggt atcggggttg acggccgccc
     5761 gggtgttgcc gagcttggca agcgggtagg ctttgcgcgt taccgcgccg aggacggaag
     5821 ttttgctgtt ttcccaactg tcctgatcga aatcgctgcc taccggctgc tgccagtttt
     5881 ccagcgcgtt gcggtagtat tcggcgaggt tgccgtctga aaggatgtcg ccgaggatgg
     5941 cgacgccgac gttggcgatt ttggtgcgcg ggcccggtgt gatgtgcacc gtataagctc
     6001 cgtctttttc cgtcaggctg actttgctgc tgaaatagcc tttgctgcgg agcatcgttt
     6061 taacgttgtc cggcgcttct tcggcgagga agcctgtctg ttccttgtcc aatacttctt
     6121 cctgctgctg cgtgatgagc ggcaggtgtt cttcgaccat atctttgatt tcactgtcct
     6181 gcgtgtcgat gcggacgggg aatttgggtt ttaatttgac tgattcggtg tcagggcttt
     6241 tgtttttgaa caatgcgaaa cccgccgact tgttttcgga aaggtcggcg gcaggcgcgt
     6301 atgcgtgcgg aaagaaaaat aaagccggca ggagcagggc ggtcggtttg atcatcatgg
     6361 tacgggtgtc gggtatcggt gtggcggctt tcgattttaa cactgttcgg acggggcggg
     6421 acgcttcaga cggcatactg ccgccggtat ttcggaggtt gcggcccgcc ggaaatgtat
     6481 ctgcttgttt taaggtattt tgtttccaat ctgcatatgg aaaacaggct gcgtttcgga
     6541 atagtgtcga aaaataaacg ggattaaata aatttatttt cgttcaataa aaaaccgttg
     6601 cgccgaatga taaagcaaag cctgtccggc actgcatagg gcaaatcaac gcaaccttca
     6661 tagtggatta acaaaaaccg gtacggcgtt gcctcgcctt agctcaaaga gaacgattct
     6721 cttaaggtgc tgaagcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc
     6781 gtcgccttgt catgattttt gttaatccac tataaaacac agccgaaaaa taaaaaaaca
     6841 cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac ataaggaaga
     6901 attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct ggtcaggcag
     6961 attgccgccg gtttggcgtt gggtatcgta atcggttcgg tttccccgca actgggcttg
     7021 gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc accggttttg
     7081 gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa ggcgcatatc
     7141 aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac cgccgtcatc
     7201 gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt gtccgccgcg
     7261 ccgccttccg gcattgtgga agtgttgaaa tcgctgctga tgaacctggt cgccaacccg
     7321 attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt ggttttgggc
     7381 gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga tttggcggaa
     7441 gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat tttcgggctg
     7501 gtgtcatcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc gaagctgctt
     7561 gcggtgctgt tgggctgtat ggcgtttatc gcgctagtgg tcaatcccgc catcgtgtgg
     7621 tggaaaatcc gccgcaatcc ttatccgctg gtgtttacct gcctgcgcga aagcggcgtg
     7681 tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc tttggcgaaa
     7741 aagctggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc gaccgtcaat
     7801 atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgca cacccaaggc
     7861 atacaggttg attttgccac cgcgctactg ttgagcctgg tggcgacggt cagcgcgtgc
     7921 ggtgcgtccg gcgtggcggg cggttcgctg ctgctgattc cgctggcgtg cagcctgttc
     7981 ggcatcagca acgatgtcgc tatgcaggtg gtcgccgtcg gctttatcat cggcgtgatt
     8041 caggattcgg cggaaacggc gttgaattct tcaaccgacg ttttatttac cgctgccgcc
     8101 gatttgggcc gacagagaaa ccgggcggaa taaaccaagc tatgccgtct gaagcccgtt
     8161 tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac aataccggct
     8221 gccttaaata tgaaggagat gatgatgttt gaacgcagtg tgattgcaat ggcttgtatt
     8281 tttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa gtcggcggac
     8341 acaccgtcaa aaccggccgc tcctgttgtt gctgaaaaag agacagaggt aaaagaagat
     8401 gcgccacagg caggttctca aggacagggc gcgccatcca cacaaggcag ccaagatatg
     8461 gcggcagttt cggcagaaaa tacaggcaat ggcggtgcgg caacaacgga caaacccaaa
     8521 aatgaagacg agggaccgca aaatgatatg ccgcaaaatg ccggcaatac ggctgcccaa
     8581 ggtacaaatc aagccgaaaa caatcaagtc ggcggctctc aaaatcctgc cccttcaagc
     8641 aatcctaatg ccacgaatgg tggcaatttt ggaagggttg atttggctaa cggtgttttg
     8701 attgacggac cgtcgcaaaa tataacgttg acccattgta aaagcgattc ttgtaatggt
     8761 gataatctat taagcgaaga agcaccgtca aaatcagaat ttgaacaatt aagtgatgaa
     8821 gacaaaatta agaaatataa aaaagatgga gaaaagttta ccggtttggt tgctgatagg
     8881 ttacagatga aaggaaccaa tcaatatatt attttttaca aacctaaaac cacttcatct
     8941 gcgcgattca ggcgttctgc acggtctagg aggtcgcttc cggctgagat gccgctgatc
     9001 cccgtcaatc aggcggatac gctgattgtc gatggggaag cggtcagcct gacggggcat
     9061 tccggcaata tcttcgcgcc cgaagggaat taccggtatc tgacttacgg ggcagaaaaa
     9121 ttgtccggcg gatcgtatgc cctcagtgtg caaggcgaac cggcaaaagg cgaaatgctt
     9181 gctggcacgg ccgtgtacaa cggcgaagtg ctgcatttcc atacggaaaa cggacgttcg
     9241 tacccgacca aaggcaggtt tgccgcaaaa gtcgatttcg gcagcaaatc tgtggacggc
     9301 attatcgaca gcggcgatga tttgcatatg ggtaagcaaa aattcaaagc cgccatcgat
     9361 ggaaacggct ttaaggggac ttggacggaa aatggcggcg gggatgtttc cggaaggttt
     9421 tacggcccgg ccggcgagga agtggcggga aaatacagct atcgcccgac agatgcggaa
     9481 aagggcggat tcggcgtgtt tgccggcaaa aaagagcagg attgatgttg atgccgtctg
     9541 aagcgggggc gttcagacgg cattgtgatg tttggggcgg cgagtccggt tcaagtccgc
     9601 atcagttgat aaacccgtcg tctttcattt tcttttcgga aaaatactgg ccgaatacca
     9661 gcagggcggc gaggatgatg acaaacagcc ccgccatagt cagtgtgatt ttgaagaaca
     9721 attccgcttc gagaaaagaa aaccagagtt gcacaatcag cagcagcacc cagccggcaa
     9781 acagtccgag acaggcataa acgccgagct taaacacggt cccgctccaa aatgacgacg
     9841 taggcctcgc gtttgaaaaa cagcagcata cggcgcaaat ctttttccat cgttacga
//
LOCUS       DAWHXS010000070         9819 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWHXS010000070 DAWHXS010000000
VERSION     DAWHXS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.070"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(527..1969)
                     /gene="aldA"
                     /locus_tag="WIE22_001426"
     CDS             complement(527..1969)
                     /gene="aldA"
                     /locus_tag="WIE22_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ4127497.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(2004..2204)
                     /locus_tag="WIE22_001427"
     CDS             complement(2004..2204)
                     /locus_tag="WIE22_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127498.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(2213..2602)
                     /locus_tag="WIE22_001428"
     CDS             complement(2213..2602)
                     /locus_tag="WIE22_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127499.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2603..2779)
                     /locus_tag="WIE22_001429"
     CDS             complement(2603..2779)
                     /locus_tag="WIE22_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127500.1"
                     /translation="MSGYVQFLGTDSKGQSKFIFVGTNENGSITTIHTKSGKDFWRTL
                     NNNPKNKTIYPKAR"
     gene            complement(2810..3727)
                     /locus_tag="WIE22_001430"
     CDS             complement(2810..3727)
                     /locus_tag="WIE22_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4127501.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYMEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLNFGINRSRFQIDADGGKVILISVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYACFTPILYREPVRIWNLSPNLRGLAASCLQAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLADALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3900..4700
                     /locus_tag="WIE22_001431"
     CDS             3900..4700
                     /locus_tag="WIE22_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4127502.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4748..5524
                     /gene="mlaE"
                     /locus_tag="WIE22_001432"
     CDS             4748..5524
                     /gene="mlaE"
                     /locus_tag="WIE22_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ4127503.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5575..6069
                     /gene="mlaD"
                     /locus_tag="WIE22_001433"
     CDS             5575..6069
                     /gene="mlaD"
                     /locus_tag="WIE22_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ4127504.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVGFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPIKSAGVLVGRVGSIELDPKSYQAKVRLDLDSKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            6106..6696
                     /locus_tag="WIE22_001434"
     CDS             6106..6696
                     /locus_tag="WIE22_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ4127505.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDTQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6747..7025
                     /locus_tag="WIE22_001435"
     CDS             6747..7025
                     /locus_tag="WIE22_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ4127506.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            7022..7849
                     /locus_tag="WIE22_001436"
     CDS             7022..7849
                     /locus_tag="WIE22_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ4127507.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7974..8336
                     /locus_tag="WIE22_001437"
     CDS             7974..8336
                     /locus_tag="WIE22_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ4127508.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            8333..8719
                     /locus_tag="WIE22_001438"
     CDS             8333..8719
                     /locus_tag="WIE22_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ4127509.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8716..9204
                     /locus_tag="WIE22_001439"
     CDS             8716..9204
                     /locus_tag="WIE22_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ4127510.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(9287..9454)
                     /locus_tag="WIE22_001440"
     CDS             complement(9287..9454)
                     /locus_tag="WIE22_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ4127511.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9421..9801)
                     /locus_tag="WIE22_001441"
     CDS             complement(9421..9801)
                     /locus_tag="WIE22_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ4127512.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2278 a         2705 c         2459 g         2377 t
ORIGIN      
        1 aaagtgggaa tgacggttca gttgctacgg ttactgtcag gtttcggtta tgttggaatt
       61 tcgggaaact tatgaatcgt cattcccgcg caggcgggaa tctagtctgt tcggtttcag
      121 ttatttccga taaatgcctg ttgcttttca tttctagatt cccactttcg tgggaatgac
      181 ggaaagtggc gggaatgacg gttcgggcat tccttaaatc acccgtgtat cgctgtaaat
      241 cttagagatg gcggaatata gcggattaac aaaaaccagt acggcgttgc ctcgccttag
      301 ctcaaagaga acgattctct aaggtgctga agcaccgagt gaatcggttc cgtactatct
      361 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat acaaagacaa
      421 aaacatcgat gtcggtcatg actgattgcc ggtgaaactg taaaaatgcc gtctgaacct
      481 gaaaacgggt tcagacggca ttttctatcg gggttttaaa gcggcattaa atgtcggttt
      541 ccagataaac gacttgggtt tgcagatatt cttccaaacc gtgtttgccg tccgcgccgc
      601 cgatgccgga ttttttccag cccgcgtgga agccttgcat ggcttcaaag ttttcgcggt
      661 tgatgtaggt ttcgccgaat tgcaggcggc gggtaacgta gaaggcttcg tttaaattag
      721 tcgtataaac agaactggtc agaccaaact cgcaatcgtt agccagtgcg atgacttggt
      781 caagcgtgtc gaaagcggaa acgggcagaa cggggccgaa ggtttcttct ttcataatgt
      841 ccatactgtt gtcggtgtcg gtcagcaggg tcggctcgaa gaaataaccg cgtccttcgg
      901 cgcgtttgcc gccgcagacc agtttcgcac cttgtttgac tgcccgttcc actttttcgg
      961 caacggcttt gacggcgcgc tcttcaatca gcgggcccat ttccagcgcg cctgcttcgg
     1021 cttcggcagg gttgccgtag cgcacgcctt tcatggcggc agtcattttt tcaatgaatg
     1081 cgtctttcag actgctgtgg acataaacgc gctcggcgca gttgcagatt tgaccggtgt
     1141 tgccgacacg cgaagccaag atggatttca ccgccaaatc caaatccgcg tctttcaaaa
     1201 cgatggcggg ggctttgccg ccgagttcca acgaaacttt ggtgatgttg gcggaagcgg
     1261 cttccatcac ttggcggccc gcttcgacgg agccggtcag gctgaccata tcgacttgcg
     1321 gatgggcgga caaggcattg ccgatttccg cacccggacc gttcacaacg ttgaacacgc
     1381 ctgctggcag tccgaccgca tcgacgattt cggcgaagat gtggcagttg atcggggtta
     1441 cgctgctggg tttgacgacg atggtgttgc ccgttaccaa agcggggccc attttgcggg
     1501 caatcaggaa gaaggggaag ttccacggca aaatgccggc gattacgccc agcggacgtt
     1561 tgaacaataa aatattttcg cgcgggcggt cgctttggat gatttcgcct tcgtaacgac
     1621 gcgcccattc ggcttggtaa tcgagatagt cggcggtaaa catgacttcc acgcgcgcca
     1681 agtctttggt tttgccgcct tcggcaacga tggtgtcggt cagctcgtcg gcgcgtccgc
     1741 gtatgccttg ggcgattttg cgcaaatacg cgccgcgttc gaccgcaggc aggcgttccc
     1801 aagccggttg cgccgcgcgt gccgccgcca cagctcggtc aacgtctgct tttccgcctt
     1861 tgggttcgcg ggcgatggtt tcttcggtgg atgggttcaa tacgtcgcgc cattcgccgt
     1921 tgaaatcgtt ttcaaagcgt ccgttgatgt acatggccaa ttgtttcatt tcgggttctc
     1981 cagttttgta gtcagatgta gtcttagttt attcccaaat aaattgccgt acaagtttct
     2041 tttgaaatgt ttagacgaga agctactatg tctgcatatt gttctgctaa tttatattca
     2101 ctcgaatgca actgccgatt attccaatct gcattcgcat cagtaaaagc cccagcattg
     2161 ccgcctgtgg cataaccaat cgctaaactg ccaacggtat ccacaacagg tttcaggctg
     2221 cctgaatttc tctaataaat gaaacactta ttctatcagc cgttaatgta accaaacaat
     2281 tatcaagata agagtagttt actaaatact catcttcttc aaattgtata ttttgtaggg
     2341 ttagatgatt attttttaat tctgccgtta tttcatactt gaaactatca tctatttttc
     2401 taaatccaat atttgaagtt gatttatcca gtatgtaatc attaaagatt tcataggata
     2461 attccacttc ataaaattga ttttcaaata ttttgaatgg gcagtacgat ataaaacaat
     2521 gtagttcata accatttatt tcaatcaata ctgcctcttc aatggtgtta tctaactttt
     2581 tgattttaca tttatataac atttatcttg cctttggata aattgttttg ttttttggat
     2641 tgttattcaa agttctccaa aagtcttttc ctgattttgt atgtatcgtg gtaatactac
     2701 cattttcatt ggttccaaca aaaataaatt tagattgacc tttggaatca gtacccaaaa
     2761 actgaacata tccagacatg cggatttcgg gttttaaatc aaatgcggct taccggtttt
     2821 cttttttggt ttccgcaggc gtgctgccga aatattgcct gaatgcctga gtaaagctgg
     2881 aaacgtggcg gtagccgcac aaatacgcgg tttcgcctat gcttttgccc ccgttttgca
     2941 acaggtagag cgcgtgctgc atttgtttgt ggtgcagcca ctcgcttgcg gtaatgccga
     3001 aatggtcgcg catacggcgt tgcagcgtcc tttcgctgat gttcagcgcg tctgccagcc
     3061 ggttgacttg gtgtgcgccg tcggcaaacg cggcatttag ggtgcggctg aagtcttcag
     3121 acggcatagc gtctgcttcc gccgtttgcc ccgccgccgg cccgatgccg tctgaaaccg
     3181 tgtcccacaa atccgacagc agccgcaaaa cgtccgcctc gcggcgcaat gtttcgccca
     3241 aatgcccctt tgggacggct tgcaggcagg atgccgccaa accgcgcagg tttggggaca
     3301 aattccatat tctgaccggt tcgcggtaaa gtatgggtgt aaaacacgca tactccggac
     3361 gcagcagcca ttgctccata cctttaattg tcattttgac cgttttgccg cctcgataaa
     3421 gatagcggct aaacaggact tcttccccga ctgaaatcag gatgaccttg ccgccgtccg
     3481 catcgatttg gaatcggctc cggttgatgc cgaagttcaa agagccttcc agcaaaagca
     3541 cgaacgacac ataaggttcg gcgaggcggc tgctgcaaaa atcgcaccgt gccgttaccg
     3601 tgccgccgtg gatggaaatg ccgttggaca aagtatcgaa acggtagctg ccctccatat
     3661 agctgccgcc gccgcttttg accagattga cgaaccggct gctgttcaaa tgttctgctt
     3721 tttccatagt tttctgataa aggttatcat ttgaaagata acatttttcg ccacagcaaa
     3781 caatccgccg ccgacaacgg cattttcaca acacttaccg tccgtatgcc gtctgaaaat
     3841 tgcccgtctt cccgataagc cttaaaatac acgccgtcaa acctaccgtc cgtccgtaca
     3901 tgagcccatc cccctttatc gaaatgaaag acgtcgcctt cgcgtatggc gaccgcccga
     3961 ttctgaacga catcaatttc agtattccgc aaggtaattt tgccgccgtg atgggcggtt
     4021 cgggcagcgg caaaaccacg ctgatgcgac tgattacagg acagattcgt ccgcagtccg
     4081 ggcaggtttt gattgaagga cgtgatttgt cgggcttttc ggctgacgag ttgtatgaac
     4141 accgccgccg tatgggcgta ttgttccaac acggcgcgct gtttaccgat ttgtctgtat
     4201 ttgacaatat tgcttttccg atgcgcgaac tgacgcaact gccggaagcg gtgattcgag
     4261 atttggtttt gttgaaactg aacgcggtcg gtctgcgcgg tgtagaaaac ctgatgccgt
     4321 ccgagttgtc cggcgggatg tcgcgccgcg tcgcgcttgc ccgcacgatt gcgctcgacc
     4381 ccgaaattat gttgtacgac gaaccgttta ccggtctcga tccgatttcc ttgggcgtga
     4441 ttgcccactt gatcagccgc gtcaacaagg ctttgcgttc gaccagtatt atggtcacgc
     4501 acgacattga aaaatctttg gaaatcgtcg atcaggtgat tttcttggcg cacggcgaaa
     4561 ttatgttctc cggctcgccg caggaaatgc gcgaactgga ttcgccttgg gtgcgccagt
     4621 ttgtcggcgg cttggcagac ggccccgtgg cataccgtta tccggcgcaa acgtcgttgc
     4681 agcaggattt gctcgggtaa atgagccggt gccgtctgaa accgttgaac tttacatata
     4741 gaaatttatg aactttatcc gttccgtcgg ggcgaaaacc ctcggcctta ttcaatccct
     4801 cggcagtatt acgctgtttc tgctgaacat cttggcgaaa tccggtacgg ctttcgtccg
     4861 tccgcgcctg agcgtgcgcc aagtgtattt cgccggcgtg ctgtcggtgc tgattgttgc
     4921 cgtttcgggg ctgttcgtcg gtatggtttt gggtttgcag ggttatacgc agttgtcgaa
     4981 attcaaatcc gccgatattt tgggctatat ggtcgcggct tcgctattgc gcgagctggg
     5041 tccggtattg gcagcgattt tgtttgccag cagcgcgggc ggtgcgatga ccagcgaaat
     5101 cggtctgatg aaaacgaccg aacagctcga agcgatgaat gtgatggcgg taaaccctgt
     5161 cgcccgcgtg gttgcgccgc gcttttgggc gggcgtgttt tccatgccgc ttttggcttc
     5221 gattttcaac gtggcgggca ttttcggtgc gtatttggtc ggtgtaagct ggctgggctt
     5281 ggacagcggc attttctggt cgcaaatgca gaacaacatc acgatacatt acgatgtaat
     5341 caacggtctg attaaatccg ccgcgttcgg cgtggcggta acgctgattg ccgtgcatca
     5401 gggcttccat tgcgtcccga cttcggaagg cattttgcgc gccagcacgc gcacggtggt
     5461 ttcgtccgcc ctgacgattt tggcggtcga ttttatattg accgcgtgga tgtttacaga
     5521 ttgacaggcc gtctgaagac gaaacgcgga acatcggata ttcaaggaac tttaatgaaa
     5581 aagaacatat tggaattttg ggtcggactg tttgtcctgc tcggcgtggc agcggtcggc
     5641 tttctcgctt tccgcgtggc aggcggcgcg gccttcggca attccggcaa gacttatacc
     5701 gtatatgccg atttcagcga catcggcgga ctgaaggcca acgcgccgat caaatccgca
     5761 ggcgtattgg tcggacgcgt tggttccatc gagcttgacc cgaaatccta tcaggcgaaa
     5821 gtccgtctcg atttggacag caaatatcag ttcagtagcg acgtttccgc acaaatcctg
     5881 acttcgggac ttttgggcga gcagtacatc gggctgcagc agggcggcga cacggaaaac
     5941 cttgctgccg gcgacaccat ctccgtaacc agttctgcaa tggttctgga aaaccttatc
     6001 ggcaaattca tgacgagttt tgccgagaaa aatgccgacg gcggcaatgc ggaaaaagcc
     6061 gccgaataaa cgtatgccat catattcaaa ctaggacact taatcatgaa aaaatcctcc
     6121 ctcatcagcg cattgggcat cggtattttg agcatcggca tggcatttgc cgcccctgcc
     6181 gacgcggtaa gccaaatccg tcaaaacgcc actcaagtat tgagcatctt aaaaaacggc
     6241 gatgccaaca ccgcccgcca aaaagccgaa gcctatgcga ttccctattt cgatttccaa
     6301 cgtatgaccg cattggcggt cggcaaccct tggcgcaccg cgtccgacac gcaaaaacaa
     6361 gcgttggcca aagaatttca aaccctgctg atccgcacct attccggcac gatgctgaaa
     6421 ttaaaaaacg ccaacgtcaa cgtcaaagac aatcccatcg tcaataaagg cggcaaagaa
     6481 atcatcgtcc gcgccgaagt cggcgtaccc gggcaaaaac ccgtcaacat ggacttcacc
     6541 acctaccaaa gcggcggtaa ataccgtacc tacaacgtcg ccatcgaagg cgcgagcctg
     6601 gttaccgtgt accgcaacca attcggcgaa attatcaaag cgaaaggcgt ggacggactg
     6661 attgccgagt tgaaagccaa aaacggcggc aaataaccga caaaccatac cgtccgaggg
     6721 cggtatgggt tttccgagca aacgctatgc atacagaact caaaaacgga atattgcaca
     6781 tcggcggcga catcaccgtc aaaaccctga ccgccgccgc ttttacccgc ttcagacagc
     6841 aatgtcgtct gaaagacacc tgcgcggttg atttgagcgg cgtcggacgc gccgattccg
     6901 cctgcgtgtc gctgctgctc gaagtgctgc gcggttgcaa aggcagcgtc aggctgaccg
     6961 gcattcccga atccgtgcgc gcgctgtccg aactgtacga aatcaaagac tggctgaaat
     7021 catgaaaaaa accgcctatg ccttcctcct gctgatcggg ttcgcttccg cccctgcatt
     7081 tgccgaaacc cgccccgccg acccttatga aggctacaac cgcgccgttt tcaaattcaa
     7141 cgaccaagcc gaccgctaca ttttcgcccc tgccgcgcgc ggctaccgca aagttgcgcc
     7201 gaaacccgtc cgcgccggcg tgtccaattt ttttaacaac ctgtgcgacg tggtcagctt
     7261 cggtagcaat atcttgcgct tggacatcaa acgcgcaagc gaagaccttg tccgcgtcgg
     7321 catcaacacc actttcggtt tgggcgggct tatcgacatc gccggcgcgg gcggcattcc
     7381 cgacaataaa aacaccttgg gcgacacgtt tgcttcgtgg ggatggaaaa acagcaatta
     7441 tttcgtgttg cccgtcttag ggccgtccac cgtccgcgac gcgctcggca cgggtattac
     7501 ctccgtttat tcgcccaaga atatcgtctt ccgcacccct gtcggacgct ggggcacgac
     7561 tgccgtatcc gccgtcagta cgcgcgaagg tctgctcgat ttgaccgaca gtctggacga
     7621 agccgccatc gacaaataca gctacacgcg cgacctctat atgaaagtcc gtgcgcggca
     7681 gaccggtgca acacctgccg aaggtacgga agataacatc gacatcgacg aattggtcga
     7741 aagtgccgaa accggcgcgg cggaaactgc cgttcaagaa gattccgtat ccgaaacaca
     7801 ggcagaagca gcaggggaag ccgaaacgca acctggaaca caaccttaaa gccatgccgt
     7861 ctgaaaatcc gtgttcggac ggcattccct tctgtcggaa cggacagtcc gaaaccacag
     7921 attgggctat aatctctttt ttgcgcatac cgcgcgacag caaaggaaaa accatgtacg
     7981 aagtcaaccg cagcgtattc gtcctcatcc ctttagaacc tttttggaac tggctgcaaa
     8041 ccctgccggg caaccacctc gacggactga ctcttgaaga catccaagcc gacgccaatt
     8101 cctaccttgt ccgcccgtgc gaaaccgccg acgaagtatg ggacgaaatc gaagcccgtt
     8161 ttgaagacat tttcgccgcc gaacttgccg actggtgcga agacgaacgc gagtggcctg
     8221 cgctcgatgc cgacattttc aacgaatggt tcgacatcca gctttctacc gtcatcaccg
     8281 acctcgaaca cgaaccgctt gcccgcgaag ccttccaacc catcaacctg aactgatgaa
     8341 gctgaccgtc cgcaactacc atctcgacgg ctacggccat gtcaacaatg cgcgctacct
     8401 cgaatttttc gaagaagcgc gctgggcgtt ttttgaagaa cgcggactgc tgcacgagtt
     8461 ggcaggcctc atactgattg tcgcccgcat cgacatccga tacagccgcc ccgccgtcga
     8521 aggcgacgta ttgcagtttt catgccgcct gaaaaccccc ggcacgcgcc gtattgtgct
     8581 gacccagacg attacactgc caaacggcaa aaccgccgca gaagccgaca tcaccctgat
     8641 gcccgtccac gccgccacac aacgcaccgt cagcctgccc gccacccttg cccgcgcatt
     8701 ggaagccttg tccgaatgaa aaaaatactc accgccgccg tcgtcgcact gatcggcatc
     8761 ctccttgcca tcgtcctcat ccccgacagc aaaaccgcgc ccgccttctc cctgcccgac
     8821 ctgcacggaa aaaccgtttc caacgccgac ctgcaaggca aagtaaccct gattaatttt
     8881 tggtttccct cctgtccggg ttgtgtgagc gaaatgccca aaatcattaa aacggcaaat
     8941 gactataaaa acaaaaactt ccaagtcctt gccgttgcac aacctatcga tccgatagaa
     9001 agcgtccgcc aatatgtcaa agactacggt ttgccgttta ccgtcatgta tgatgcggac
     9061 aaagctgtcg gacaggcgtt cggcacacag gtttatccga cttctgtcct catcggcaaa
     9121 aaaggcgaaa tcctcaaaac ttatgtcggc gaacccgatt tcggcaaact ctaccaagaa
     9181 atcgataccg cgctggcaca atagcgatgc cgtctgaagc gcaaaaaaaa cagaaacgat
     9241 gttcctgttt tttcagatca tccgccccga cacgttcaga cggcattcaa ccttccggca
     9301 gattcttttt caccactgcc ggattgcctg ccgccaaaga gtagggcgga atgtcttttg
     9361 taaccaccgc gcctgcgccc acgaccgaac cgcgtccgac ggttacgccc gccattacaa
     9421 tcaccctgtg ccccggccag acatcgtcct ccaacgtaat cggacggatt tccgtgtagc
     9481 cctcaaagcg ttttttgaac ggtcaaactt gtggttattt gaataaaaca gacattccgg
     9541 ctccatcatc acatttttgc cgaccaccag cccacggcag atttcacagt ttgccccgat
     9601 gcccgagccg tcgcccaaaa ccgtatccgg aaacacatac gccccgcgtt cgatattgac
     9661 cccgcgtccg atatgcggag aaacccgccg cgccaaaaaa ccgcgcaccc gtctgccgac
     9721 gatgccgatg ccgcgcatat gtgacggcgg caaccccgaa ccggcaaaac gcagcagcag
     9781 gaaaaagaat tttgatccca tcaaacctcc ctcaaaaga
//
LOCUS       DAWHXS010000071         9799 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWHXS010000071 DAWHXS010000000
VERSION     DAWHXS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.071"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(13..1308)
                     /locus_tag="WIE22_001442"
     CDS             complement(13..1308)
                     /locus_tag="WIE22_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ4127513.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVAGAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WIE22_001443"
     CDS             complement(1434..2321)
                     /locus_tag="WIE22_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ4127514.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="WIE22_001444"
     CDS             complement(2321..3286)
                     /locus_tag="WIE22_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ4127515.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WIE22_001445"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WIE22_001445"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ4127516.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLSNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4622..4729)
                     /locus_tag="WIE22_001446"
                     /pseudo
     CDS             complement(4622..4729)
                     /locus_tag="WIE22_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WIE22_001447"
     CDS             complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WIE22_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ4127517.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WIE22_001448"
     CDS             complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WIE22_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ4127518.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGTALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WIE22_001449"
     CDS             complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WIE22_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ4127519.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WIE22_001450"
     CDS             complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WIE22_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ4127520.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8698..>9799
                     /locus_tag="WIE22_001451"
     CDS             8698..>9799
                     /locus_tag="WIE22_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ4127521.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2498 a         2609 c         2435 g         2257 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgtgc ataaaaaaca tttgcggcca aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcgtcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctaacgg caatcgcctt
      541 gcgttccaaa ggctggaata tcgggttcaa acctgcatag gtattgacgg catacaccag
      601 atttttgcat tcgacgctgc cttcgggcgt gtaaaccagc caaccgtttt gatgcggttc
      661 gatgcgcgtc atcggggact gctcgaaaat ctgcgcgccg gcttcggcag cggcaccggc
      721 aacacccaac gtgtaattga gcggatgcag gtgtccggat aaggggtcga attgtgcccc
      781 ttggtacata tcgctgtcaa gctgctgttt caactcggct ttatcccaaa gttgataatg
      841 actcgcaccg taatgccgtt gggcgtgttc atgccactgc tgcaactctt cccaatgctg
      901 cggacggacg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcctg
     1021 ctccaagccg acctgttttt caatttcccc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcaggtgta ttttcggacg gaatgataca ggctgtcggg ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatt
     1561 aggatggtcg ccaagacatt catctgcgga tcgagaccca acttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gactggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtacgc catacacagc
     1861 gtcgtatgtc cgaggaagat ggtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagtgtgc ccaaaacgac ggcagcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa tgccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacagac
     2941 aaattaccgt cgtagtcagc gcagtcttaa ttgaagacca ataagtcatc agatagatat
     3001 tgctgttttc accgtcgccg aacggattga gcgtactcca aaaattttgg aagatgtctg
     3061 cataattttg gtagctgaca gcaatattca gacgacccaa atcctcatct atcgtcgtta
     3121 aaggagtaaa cggcgggatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accgcacgct
     3241 gccccggacg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa cagtggcgta ggctggtttt ccggccagct gatatagacg gtttcgtccc
     3361 aagttggcgg tgtaatgttg cgcacatacc agtaaggggc ggggacttgg ctcttgacga
     3421 cgcgcccgtt ggagagtttg atatggtata tggcgaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 ggtcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcggaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgtgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgc tggcgcagtt
     3901 ttttgtccaa cgcgccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac cgccggacaa ttggtgcggt ttgcgtttag
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccgaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcgatc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata atttttccct gattgggact ttccataccc gccagcatac
     4261 gcagcagcgt ggattttccg ctgccggaac tgcccaaaag agcgaaaatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaaacctt
     4381 ggatttgcag ataaggcttg gttgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaga cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agtcgcaacg tctgatgccg tctgaaactc ttgtgaagcg cacgggcagc
     4561 atgaaatgga acaagattcc aaagaacttt atattatatt agtttatgcg gttttcgggc
     4621 aatatagtgg attaaattta aaccagtaca gcgttgcctt gccttgccgt actatttgta
     4681 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactataa atatggattt
     4741 gagcttgtcg gaaagcaaca gaaaagaaaa caccgcccat ttttctgggc ggtgtcggaa
     4801 agcgtaatta tttacgcaga cccaagcggg taatcaacgc gcgatacgta tcgggctggg
     4861 tacggcgcaa gtaggccagc aggcggcggc gttggctgac cattttcaac aggccgcgac
     4921 ggctgtggtg gtcttttggg ttggctttga agtggggggt caggtcgttg atgcggaaag
     4981 tcaacagagc gacttgtact tcggaagagc cggtgtcgcc ttctttgcgt tggaaatctt
     5041 taacgatttg tgctttttgt tctacggtca gtgccataat gaaaactcca aaaatataag
     5101 aatccccata gggattcaga caagtttgcc aagcctgaag acaacggcaa actccctatg
     5161 cccaagatag gacaacgtgg cattatgaca caattcccgc atttctgcac aatattttaa
     5221 gaccgtggaa gtaaccgtta aaaatgccgt ctgaagcatt gtctgcttga gacggcaatg
     5281 ttcagacggc atatgcgctt caaaccacga tttcttcttt cttcttcggt tctttaccga
     5341 tattgtcgcg gctcaaaccg aacattagca gaagcgggct ggcaaccaat acggaagaat
     5401 aaatgccgaa cacgatgccg atggtcaacg ccatagaaaa gccgtgcaag gccgtaccgc
     5461 cgaacaccag catggatacg accatcgcct cggtcgaacc gtgggtaatg atggtgcggc
     5521 tcatcgttgc ggtaatcgcg ttgtcgatga cttccggcac ggcatgtccg cgcatcgccg
     5581 gcttgcggaa gttttcacgg atacggtcga agacgacgac ggattcgttc acagaatagc
     5641 ccaatacggc aaggataccc gccaagacgg tcagcgaaaa ttcccattgg aagaaggcaa
     5701 agcagccgag aataatcacg atgtcgtgca tattggcgat aatggcagat acggcaaaac
     5761 gccattcgaa acgcatcgac aggtaaataa tgatgccgat aacgacaaaa cctaaagcca
     5821 tcaatccatt acttaccaat tcctcaccaa cttgcgggcc gataaattcg acttggcgca
     5881 aagtaacgtc gggactgtct tttttcagca aatccataac ctgattggac aactgtgcgg
     5941 aagtaacacc ttctttgttc ggcaggcgga tcatgatgtg tttgttcgta cccaatgcct
     6001 gaacctgtac atcacctatt ttcagcgtat cgaggcgttc gcgcatctta ttgacatccg
     6061 caccctgctg atattggact tccattaccg tgccgccggt aaattcgaca gagaaattca
     6121 gacctctggt aaccaaaaag aatacggcgg caataaaggt aaccaacgaa ataaaggtcg
     6181 tcagtttgcc gtagctcata aacggaatat cgcgtttgat tttaaagagt tccatagctt
     6241 actccttgcc tcctgccatt tcggctttcg gcttccacac cgaaccgatg gaaatattct
     6301 gcaatttgcg tctgcgtcca taccacagat tgaccaacgc acggaatacg acgacggacg
     6361 aatacatcga agtcagaata cccaaacagt gtacgaccgc aaaaccgcgt accgggccgg
     6421 aaccgaatac caaaagcgcg ataccggcaa tcagcgaagt caggttggaa tcgacaatgg
     6481 tcgcccatgc gtgttggaaa ccgagattga ttgcctgctg cggcggcacg ccggcacgca
     6541 attcttcgcg gatacgttcg ttaatcaaga cgttggagtc gattgccata cccaaagtca
     6601 acgccagcgc ggccataccc ggtaacgtca acgttgcctg catggcagac aaaataccga
     6661 ttaggaacag tatgttggca ctcaatgcaa tggtagaaaa gaaacccatc agacgatagt
     6721 aaaccaccat gaatgcagca acgatggcaa aaccccataa agtcgaatgg aagccttttt
     6781 cgatgttctc cttacccaaa gacggaccga tggtacgttc ttcgacaatc tgcatcggcg
     6841 cggcaagaga accggcacgc aacagcaaag acgtatcatt ggcttcggct gtcgtcatgc
     6901 ttccggaaat ttccacgcgt ccgccggtaa tggcagtacg gataaccggc gcggttacaa
     6961 cctcggattt tccttggtcg atcaaaacca tcgccatgcg tttgccgaca tttgcggcag
     7021 tcagttcgcc aaaaatgctg ccacccgcgc tgtccaagct cagactgaca gcaggtgcgc
     7081 ccatttggtc gaaactcggt tgcgcatcgt tgatgttgtc gcccgtcagc tcgacctgtt
     7141 tgctgattag cagagtttcg ggacgatctc cgccgcttga aagcagctca taaccgctcg
     7201 gcacgttgcc ttccaatgcc tcgcgcaact tggcaggatc gtcctccacc atacgcaatt
     7261 ccaaagtcgc ggtacggccg atgatgtctt ttgccttggc agtatcctga acgcccggaa
     7321 gctgcacgac gatacggtcg gcaccggact gctggatgac gggttcggcc acgcccaact
     7381 cgttcacacg gttgtgcagg gtagtgatgt tctgtttgac cgcatcggaa cgcactttat
     7441 tgaccgcctc ttccgaaagc gtcaagacga tattgctgcc gtctgaattc agcgttgctt
     7501 caggaaacag cttgcgcaac tgcggcagtg ccttttgcac atcacctgca tcctgcaaag
     7561 ggacggtcag gctgtttcca gcctgacgca ccgtgccgct gcggattttt tcgcggcgca
     7621 gttcgcggcg gatgtcgccc gaataacgtt caaacgtttt ctgcatcgcc gctttcatat
     7681 cgacctgcat ggtgaaatgc acgccgccgc gcaggtccaa acccaaaaac atcggattgg
     7741 ctttgatttt cgccatccat tcggggctgt ccgccaacag gttgagcgcg gtaatatacc
     7801 cttcgcccaa agtgttttcg atgacgtcgc gcgctttaag ctgcgtttct gtgtctttga
     7861 aacgcacttt cagtgaattg tccacaacaa acatcccgtc ggtctgaata cctgcgtttt
     7921 tcagcgcggc atccactttg gattgagtct gttcgttgat gatgatggct tgtcggttgg
     7981 tcgatacctg cacggcgggt gtttcgccga ataggttggg cagcgaatac actgcggcaa
     8041 ccgcaatcgt gaacacaatc agcagatatt tccataaagg ataacggttc atcattgttc
     8101 cttaatggtt ggaaccccac cctttcggtg gtgtcggaat cgggctattt cagaagaggc
     8161 aaaaaccctt cccagccagg caagaccgga aggcggcatc ttgaatatgc cgccctgcgt
     8221 gtcggaacat ggtcaagcct tcggttggaa ttcaaaacaa agtgccgcat tcgggctttc
     8281 cagatgcggc ttgtcggcac aaatcaatcg acttttgcgg caatcgcatt gcgttctact
     8341 tcgacctcga tttttgtacc ctgtccgata tccacggtaa aaaactgttc gccgactctg
     8401 gttaccttac ccttgaaacc tgccgccaag accactttgt cgccgacttt caaggcggca
     8461 agcattgcct gatgcgcttt gaatttcttt tgctgcggac gcatgatcag gaagtagaac
     8521 accaccataa tcaacactaa aggagcaaat tgtgcaacag cttgattcat aatttatccg
     8581 ttctttctaa tatggttgaa aatcgagagg ggtatataat aacataagac cgtaaacaat
     8641 atatcgggtt tgccgtccgt accgaccgta taccgcagcc tgcccgtcca caaacccatg
     8701 tccttgaccc gcctaatctt gaaattctat gcactgttgc gccttttttt gggcaaaaac
     8761 gcccgcaccg catggatttc gcatcccgcc tgtgccgggc acgaacccgg cgcaaaccat
     8821 cccgattcgc ccgaccgcat cctctgcatc gagcaggcat tgcgccgcgc cggtatttgg
     8881 cagcacctcc aaaccataga ggcggaagaa atcagcgata cgcgcctcgc acttgtccac
     8941 tcgagcaaat atctgaaccg tttggaatct tgcctgcccc aaaaaggcaa gattttccgc
     9001 ctggataacg acactgcaat cagcacaggc tcgctgtctg ccgcacactt tgccgccggt
     9061 tcggcagttc aggcagtcga catggtcatg aaccgtaaag catggcatgc cttttgcgcc
     9121 gcccgcccgc ccggacacca tgcgggcagc ggcaaagccg gcggattctg cctgctgaac
     9181 aacgttgccg ccggcgtcat gcatgccatt gccgaatacc gcctgaaacg cattgccgtc
     9241 atcgatttcg atgtccacta cggcgacggt acggcagaaa tattcaaaga cgatccgcgc
     9301 atcctgtttt tcaacctgtt tgaaaccgac cttttcccct tccccgaaaa caacgatatg
     9361 cccgacggcg gcaatatggt gcacctgccc ttgccgccgg gaacgggcag ccgcacattc
     9421 cgcgaagccg tccgcagaca gtggctgccc cggcttgccg cattcaaacc cgaactggtg
     9481 ctgctgtcgg caggattcga cgcacaccgt ctagacgaat cgggcaggct caacctgcac
     9541 gaggcggatt ttgcctggct gacacacaaa atcattcaga cggcatcggg ctgccccggc
     9601 aaaatcgtat ccgtactcga aggcggctac acccttgaac ctctggcaca atctgctgcc
     9661 gagcatatcc gcgtgctggc ggggctgggc aaatccgatg ccgcaaccgc ctatcagaaa
     9721 acactggacc caacaaaaaa acggtttgcc aaaccgaaaa ccggacaggt gcgccaacct
     9781 acccaatccg accgttacg
//
LOCUS       DAWHXS010000072         9747 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWHXS010000072 DAWHXS010000000
VERSION     DAWHXS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.072"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            71..598
                     /locus_tag="WIE22_001452"
     CDS             71..598
                     /locus_tag="WIE22_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ4127522.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            686..1741
                     /gene="plsX"
                     /locus_tag="WIE22_001453"
     CDS             686..1741
                     /gene="plsX"
                     /locus_tag="WIE22_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ4127523.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            1877..2149
                     /locus_tag="WIE22_001454"
     CDS             1877..2149
                     /locus_tag="WIE22_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ4127524.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESQEISFIDKEFRTESTLDYPIKSTG"
     gene            2155..2571
                     /locus_tag="WIE22_001455"
     CDS             2155..2571
                     /locus_tag="WIE22_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127525.1"
                     /translation="MKTSTVVFGGFFITDNGERIQIPVLENPGIKEINHFFSISNFEK
                     KAGVLVFRIIPEPEFGDTELTIYFEKGYYLPMIQTILEDGDIEVKNLKTENYSGKARE
                     ILGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            2591..2794
                     /locus_tag="WIE22_001456"
     CDS             2591..2794
                     /locus_tag="WIE22_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127526.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            2821..3783
                     /locus_tag="WIE22_001457"
     CDS             2821..3783
                     /locus_tag="WIE22_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ4127527.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDATGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3830..4201
                     /locus_tag="WIE22_001458"
     CDS             3830..4201
                     /locus_tag="WIE22_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ4127528.1"
                     /translation="MKRQAFFKPIACAAFLSAISLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIDYFVPPELFVKLGQHL"
     gene            4227..5153
                     /gene="fabD"
                     /locus_tag="WIE22_001459"
     CDS             4227..5153
                     /gene="fabD"
                     /locus_tag="WIE22_001459"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ4127529.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            5292..7157
                     /gene="msbA"
                     /locus_tag="WIE22_001460"
     CDS             5292..7157
                     /gene="msbA"
                     /locus_tag="WIE22_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ4127530.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            7257..8822
                     /gene="guaA"
                     /locus_tag="WIE22_001461"
     CDS             7257..8822
                     /gene="guaA"
                     /locus_tag="WIE22_001461"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ4127531.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            8884..9630
                     /gene="fabG"
                     /locus_tag="WIE22_001462"
     CDS             8884..9630
                     /gene="fabG"
                     /locus_tag="WIE22_001462"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ4127532.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         2484 a         2990 c         2330 g         1943 t
ORIGIN      
        1 cgaggcaacg ccgtactggt ttttgttaat ccgctatatt ccaaaagccc caaccaccaa
       61 ggaaattccg atgaaacaga aaatctggta cacctacgat gacatccacc gcgtcatcaa
      121 agcattggca gaaaaaatcc ggaacgccga catcaaatac gatgccatga ttgccatcgg
      181 cggcggcggc tttattccgg cacgtatgct gcgctgtttt ctggaaattc cgatttatgc
      241 cgtaaccacc gcctattacg acagcgacaa cgaaggacag gttaccgaag aagtcaaaaa
      301 agtccaatgg ctagaccccg ttccagaagc cctgcggggc aaaaacgtac tcgtcgtcga
      361 tgaagtggac gacagccgcg taaccatgga attttgcctg acagagctgc tcaaagaaga
      421 cttcggtacg atcggcgtcg ccgtactgca cgaaaaaatc aaagccaaag caggcaaaat
      481 ccccgaaggc attccctatt tcagcggcat caccgtagaa gactggtgga tcaactatcc
      541 gtgggacgca ctcgacatcg acgaacacaa ccgccttgcc gaggccggcc gaggctgacc
      601 ctttcagacg gcatattttc cgaaccgatg ccgtctgaag cccgcacgac ccctgccgca
      661 gaccgaaaac ctaccggaga accctatgat tacacttgcc gtagatgcca tgggcggcga
      721 ccaaggactt gccgttaccg tacccggcgc aaccgcattc ctccaagcac accccgatgt
      781 ccgcctgatt atgaccggcg acgaaacgca actgcgccaa gccctgaccg cggcaggcgc
      841 accgatggaa cgcatcgaca tctgccatac cacccaagtt gtcggcatgg atgaatcccc
      901 tcagtccgcc ctaaagaaca aaaaagactc ctccatgcgc gttgccatca accaagttaa
      961 agaaggcaaa gcccaagccg ccgtatccgc aggcaacacg ggcgcactca tggcaaccgc
     1021 acgtttcgtc ctcaaaacca ttcccggcat cgaacgcccc gccatcgcca aattccttcc
     1081 ttccgacacc gaccatgtta cccttgcact cgaccttggc gcgaacgtcg actgcacgcc
     1141 cgaacagctc gcccaatttg ccgttatcgg cagcgaactc gtccacgcac tccatcctca
     1201 aaaaggacag ccgcgcgtcg ggctggtcaa cgtcggcacg gaagacatca aaggtacgga
     1261 caccgtcaaa caaacctaca aactgctgca aaacagcaaa ctcaacttta tcggcaacat
     1321 cgaaagcaac ggcatcctgt acggcgaagc agatgtcgtc gtcgccgacg gctttgtcgg
     1381 caacgtcatg ctcaaaacca tcgaaggcgc ggtcaaattc atgagcggag ccatccgccg
     1441 cgaattccaa agcaacctgt tcaacaaact tgccgccgtt gccgccctac ccgccctcaa
     1501 agggctgaaa aacaaactcg atccgcgcaa attcaacggg gccatcctgc tcgggctgcg
     1561 cggcatcgtc atcaaaagcc acggcggcac agacgaaacc ggtttccgct acgcactcga
     1621 agaagcctac cacgaagcca agtccgccgg cctttccaaa atcgaacaag gcgttgccga
     1681 acaactcgcc gcactcgaaa ctgccaaagc cgtccaaaac gaaaatgccg acggtctgta
     1741 acacacacga tgccgtctga acgcccccgc ccctttcaga cggcatccgc ccgcaccaaa
     1801 cctgcgggcg cggacggcga tgcgcctgtc cggcacttcc caaatacttc cctgtaaaat
     1861 aaggagtatt tgaaaaatga agacattaga aaaacggatg aaagctctag acaaacagat
     1921 gatgaagttc ggaaaatccc ttgaaggcag gcttgatgcc cgtctgattg aatccgcatt
     1981 ggattatatt cattattcgg aacgtttttt ggcttttgaa atcctgtgta cttatatcga
     2041 agatttcgat gtccggctga cggaacaaga atcccaagaa atttctttta ttgacaagga
     2101 atttaggaca gaaagtacgt tagattatcc aataaaatca acgggatgat aaacatgaaa
     2161 acatcaacgg ttgtttttgg cggatttttt attacagata atggagaacg aatccaaatc
     2221 cccgttttgg aaaatcctgg cattaaagaa atcaatcact ttttttccat atcaaatttt
     2281 gagaaaaaag ccggcgtcct tgttttcaga atcatccccg agccggaatt tggcgatacc
     2341 gaattaacta tctattttga aaaaggatat tatttgccaa tgattcaaac aattttagaa
     2401 gacggggata ttgaagtcaa aaaccttaaa acagaaaatt acagcgggaa ggcaagggaa
     2461 atcttgggag attcctaccc tattgagcat atctctaaaa atatttctac aattcaaaat
     2521 ataatttctg aatttatgat acaaaaacaa acaccggcac caataatttg atggaaaacc
     2581 aacaatcaaa atgccgtctg aagcatgttt ggttttcaga cggcatttcc ttcgcttaaa
     2641 acagcgtatc ggcaaccccg ccctgcctgt ccacggcaat ctgcatctga aaaccatctg
     2701 tatcccaaac cacaccccca tccctgtttc cgtcatttgc accctgcccg tattgggcaa
     2761 tcatcggttt ttcgcttaca atagccgaat ctgaaccaac tctctaaaaa ggccgttccc
     2821 atgcagtatg caaaaatttc cggcacgggc agctatcttc ccgccaaccg cgtcagcaat
     2881 gacgaccttg cccaaaaggt agatacctct gacgagtgga ttaccgcgcg tacgggcatc
     2941 aagttccgcc atattgcagc cgaaaatgag aaaaccagcg atcttgccgc cgaagcagcc
     3001 cgccgcgcac tggatgcaac cggattagac agcggcgaaa tcgatttaat tattgtggcg
     3061 acggcaacgc cggatatgca gtttccttct accgccacca tcgtccaaca aaaactgggc
     3121 atcaccaacg gctgccccgc gtttgacgta caggcggtgt gtgcgggctt tatgtacgcg
     3181 ctgaccacgg caaacgccta catcaaaagc ggcatggcaa aaaacgcgct ggtcatcggc
     3241 gcggaaacct tcagtcgcat tgtagactgg aacgaccgca caacctgcgt attgttcggc
     3301 gacggtgcgg gcgcggtggt tttaagcgcg tccgacaaac cgggcatcat ccacagcaaa
     3361 ctcaaagccg acggcaatta tctgaaactc ttaaacgtcc ccgggcaaat tgcctgcggc
     3421 aaagtttccg gttcgccgta catttcgatg gacggtcccg gcgtgttcaa gtttgccgtc
     3481 aaaatgctgt ccaaaatcgc cgatgacgtt atcgaagaag ccggttacac cgccgctcaa
     3541 atcgactgga tcgtgccgca tcaggcaaac cgccgcatta tcgaatcgac cgcgaaacat
     3601 ttaggtttga gtatggacaa agtcgtcctg accgtccaag accacggcaa cacatccgcc
     3661 gcatcgattc cgctggcttt ggatacgggc atccgcagcg gacaaatcaa acgcggtcaa
     3721 aacctgctgc tcgaaggcat cggcggcggt ttcgcgtggg gcgcggtatt gctgcaatat
     3781 tgaataccat gccgtctgaa gcaggttttc agacggtatt tcccatatca tgaagcggca
     3841 ggctttcttc aaaccaattg cgtgtgcggc atttctgtct gccatttcgc tgcgcctccc
     3901 cgtattgggc gcgtgttacg caatattgtc cctctatgcg tttgcacttt acggcatcga
     3961 caaacggcgt gccgtgcggg gaaaacgccg cattcccgaa caccgcctgc tgctgcctgc
     4021 cttgttcggc ggttgggcgg gcgcatactt gggcagcagg atattcaggc ataaaacggc
     4081 gaaaaagcgt tttgttgtgc tgttccgtct gactgtttca ggtaatgtcc tggcgaccct
     4141 catcctgatt gattatttcg ttccgcccga actttttgtc aaactcgggc aacatctctg
     4201 acaatccaac caaacaaagg aataccatgt cttttgcatt cttctttccc ggacaaggtt
     4261 ctcaaagcct cggcatgatg aacggcttta cagggcaggc cgtcgtcaaa aacacatttg
     4321 acgaagcatc cgccatattg ggtcaagact tgtgggcgat gattaacggt agcgatgccg
     4381 aaatcatcgg tcaaaccgtc aatacccaac ccatcatgct cgccgccggc gttgccgttt
     4441 accgcgccta tttggaagcg ggcggcaaaa cgcctgccgc cgttgccgga cacagcctcg
     4501 gcgaatatac cgcactcgtt gccgccggcg cattggattt tgccgacgcg gtcaaactcg
     4561 tgcgcctgcg cgccgaactg atgcagtctg ccgtaccgca aggcgtgggc gcaatggcgg
     4621 cgattctcgg cttggaagat gaacaggtaa aacaaatttg cgccgaatcc acacaaggcg
     4681 aagtggttga agccgtcaac ttcaactcgc ccggacaagt cgtgattgca ggcaataccg
     4741 ccgccgtcga gcgcgctatg cttgccgcca aagaagccgg cgccaaacgc gccctgccgc
     4801 tgcccgtgtc cgtaccttcc cattgcagcc tgatgaaacc cgccgccgac aaactcgccg
     4861 aagccctgaa aaccgttgaa atcaagcagc cgcaaatccg cgtcatccac aacgccgacg
     4921 ttgccgccta cgatgatgcc gacaaaatca aagacgcgct cgtccgccag ctttacagcc
     4981 ccgtacgctg gacagaaacc gtcaacgccc ttgtttcaga cggcattgcc gaatccgccg
     5041 aatgcggccc gggcaaagtg ttggcgggct tggcaaaacg catcaacaag gccgccgcgt
     5101 gcagcgcact gaccgatgcc ggacaggttg ccgcctttat cgaagcgcac tgacttcgtt
     5161 ccgcaaaaag cagcctgccc tcttcaggct gcttttcacg tccgaacggc aaacagccgc
     5221 atatttgcgc tataattgat gacgaccaaa ccaccgacag cggctgccgt tgcagttccc
     5281 gccctaccga tatgatagaa aaactgactt tcggactgtt taaaaaagaa gacgcgcgca
     5341 gctttatgcg cctgatggcg tacgtccgcc cctacaaaat ccgcatcgtt gccgccctga
     5401 ttgccatttt cggcgttgcc gccaccgaaa gctaccttgc cgccttcatc gcccccctga
     5461 ttaaccacgg cttttccgca cctgccgcgc cgcccgagct gtctgccgcc gccggcatca
     5521 tttccaccct gcaaaactgg cgcgaacagt ttacctatat ggtttggggg acggaaaaca
     5581 aaatctggac cgtccccctc ttcctcatca tcctcgtcgt catccgtggc atctgccgct
     5641 ttaccagcac ctatctgatg acttgggtct ccgtgatgac catcagcaaa atccgcaaag
     5701 atatgtttgc caaaatgctg accctttcct cccgctacca tcaggaaacg ccgtccggca
     5761 ccgtactgat gaatatgctc aacctgaccg aacagtcggt cagtaacgcc agcgacatct
     5821 tcaccgtcct cacgcgcgac acgatgatcg ttaccggcct gaccatcgtc ctgctttacc
     5881 tcaactggca gctcagcctc atcgttgtcc tgatgttccc cctgctctcc ctgctctcgc
     5941 gctactaccg cgaccgtctg aagcacgtca tttccgactc gcaaaaaagc ataggcacga
     6001 tgaacaacgt gattgccgaa acccatcagg gacaccgcgt cgtcaagctg ttcaacgggc
     6061 aggcgcaggc ggcaaaccgg tttgacgcgg tcaaccgcac catcgtccgc ctcagcaaaa
     6121 aaatcacgca ggcaacggcg gcacattccc cgttcagcga actgattgcc tcgattgccc
     6181 tcgccgttgt catcttcatc gccctgtggc aaagccaaaa cggctacacc accatcggcg
     6241 aatttatggc attcatcgtc gcgatgctgc aaatgtacgc acccatcaaa agccttgcca
     6301 acatcagcat ccctatgcag acgatgttcc tcgccgccga cggcgtatgt gcatttctcg
     6361 acaccccgcc cgaacaggac aaaggcacgc tcgcgccgca gcgtgtcgaa gggcgtatca
     6421 gcttccgcaa cgtcgatgtc gaataccgtt cggacggcat caaagccctc gacaacttca
     6481 acctcgacat cagacaaggc gaacgcgtcg ccctggtcgg acgttccggc agcggcaaat
     6541 ccaccgtcgt caacctgctg ccccgctttg tcgaaccgtc tgccggcaac atctgcatag
     6601 acggtatcga catcgccgac atcaaactcg actgcctgcg cgcccaattc gccctcgtct
     6661 cccaagacgt attcctgttt gacgacaccc tgtttgaaaa cgtccgatac agccgtcccg
     6721 acgcaggcga agccgaagtc ctgtccgccc tccaagccgc caacctgcaa agcctgattg
     6781 acagctcccc gctcggactg caccagccca tcggatcgaa cggcagcaac ttgtccggcg
     6841 gacagcggca acgcgtcgcc atcgcccgcg ccattttgaa agacgcgccg atattattat
     6901 tggacgaagc caccagcgca ttagacaacg aatccgaacg cctcgtccaa caagcgctcg
     6961 aacgcctgat ggaaaaccgc accggcatca tcgtcgccca ccgcctgacc accatcgaag
     7021 gggccgaccg catcatcgta atggacgacg gcaaaatcat cgaacaaggc acacacgaac
     7081 aactgatgtc ccaaaacggc tactacacga tgttacgcaa tatctcaaac aaagatgccg
     7141 ccgtccggac ggcataaaca aaatgccgtc cgaaatggta taatcgcccc gaccctttca
     7201 gacggcacta tatccgccga cccatccgat tatcttcaat cactgtaaaa cccattatga
     7261 cccaagacaa aatcctcatc ctcgacttcg gttcgcaagt tacccagctg attgcccgcc
     7321 gcgtgcgcga agcccacgtt tactgcgaac tgcattcctt cgatatgcct ttggacgaaa
     7381 tcaaagcctt caaccccaaa ggcatcatcc tttccggcgg ccctaattcc gtttacgaat
     7441 ccgactatca ggccgatacc ggtatttttg atttgggcat tccggttttg ggcatctgct
     7501 acggaatgca gtttatggcg caccacttgg gcggcgaagt gcagcccggc aaccagcgcg
     7561 aattcggtta cgcgcaagtc aaaaccatcg acagcgaact gacccgcgat attttcgacg
     7621 acgcgcccaa cacactcgac gtatggatga gccacggcga caaagtgtcc aaactgcccg
     7681 acggcttcgc cgtcatcggc aacaccccgt cctgcccgat tgccatgatg gaaaacgccg
     7741 aaaaacaatt ctacggcatc cagttccacc ccgaagttac ccacaccaaa caaggccgcg
     7801 ccctgttgaa ccgctttgtc ttggatattt gcggcgcaca accgggctgg acgatgccga
     7861 actacatcga agaagccgtt gccaaaatcc gcgaacaggt cggcagcgac gaagtgattt
     7921 taggtttgtc aggcggcgta gactcttccg tagccgccgc gctgattcac cgcgccatcg
     7981 gcgaccaact gacctgcgtg ttcgtcgatc acggtttgtt gcgcctgaac gaaggcaaaa
     8041 tggtgatgga tatgttcgcc cgcaacttgg gtgtgaaagt gattcacgtc gatgccgaag
     8101 ggcagtttat ggcgaaactc gccggcgtta ccgaccctga gaaaaaacgc aaaatcatcg
     8161 gcgcggaatt tatcgaagta tttgatgcca aagagaaaaa actcaccaac gccaaatggc
     8221 tggcgcaagg cacgatttac cccgacgtaa tcgaatccgc aggcgcgaaa acccaaaaag
     8281 cccacgccat caaatcgcac cacaacgtcg gcggtctgcc cgaaaatatg aagctcaagc
     8341 tgcttgagcc gttgcgcgac ttgtttaaag acgaagtgcg cgaactgggc gttgctttgg
     8401 gtctgccgcg cgaaatggtg taccgccatc cgttccccgg ccccggtttg ggcgtgcgca
     8461 tcttgggcga agtgaaaaaa gaatacgccg acttattgcg tcaagcagac gatattttta
     8521 ttcaagaatt acgcaacacc accgacgaaa acggcacgtc ttggtacgac ctgaccagcc
     8581 aagccttcgc cgtgttcctg cctgtgaaat ccgtcggcgt aatgggcgac ggacgcactt
     8641 acgactacgt cgtcgcactg cgcgcagtca tcaccagcga ctttatgacc gcacattggg
     8701 ctgagttgcc ttactcactg ctcggacgcg tgtccaaccg catcatcaac gaagtcaaag
     8761 gcatcaaccg cgtggtgtac gatgtcagcg gcaaaccgcc tgccaccatc gagtgggaat
     8821 aaccagcaaa cagggctgca ccgtccggcg cagtctttcg attatcggaa aaaaggaaaa
     8881 aatatgagca cacaagattt aagcggcaaa atcgctttgg taacaggcgc ctcgcgcggt
     8941 atcggtgcag caattgccga cacactggcg gcagccggtg ccaaagtcat cggtacggcg
     9001 accagtgaga gcggtgcggc ggcgattagc gagcggttgg cgcaatgggg cggcgaaggc
     9061 cgcgtattaa attccgccga acccgaaacc gtcgaaaacc tgattgccga catcgaaaaa
     9121 acgttcggca aactcgatat tctggtcaac aacgccggca tcacccgcga caacctcctg
     9181 atgcgtatga aagaagaaga gtgggacgac atcatgcagg tcaacctcaa atccgtcttt
     9241 cgcgcctcca aagccgtctt gcgcggcatg atgaagcagc gcgccggccg tatcatcaac
     9301 atcacatccg tcgtcggcgt gatgggcaat gccggtcaaa ccaactatgc cgcggcaaaa
     9361 gcaggcttaa tcggtttctc caaatccatg gcgcgcgaag tcggcagccg gggcattacc
     9421 gtcaactgcg tcgcccccgg ctttatcgat accgacatga cccgcgccct gccggaagaa
     9481 acccgccaaa cctttaccgc ccaaaccgcc ttgggcagat tcggcgacgc gcaagacatt
     9541 gccgatgcag tcttgttctt ggcttccgat caggcaaaat acattaccgg ccagacgctg
     9601 cacgtcaacg gcggcatgct gatgccctga cggacagccc gtgccgacaa tgccgtctga
     9661 agccctttca gacggcattt gcattcccaa acaaaatgaa cacacgccac accccgccct
     9721 gcccctgcgg atcaggcaca agctgag
//
LOCUS       DAWHXS010000073         9321 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWHXS010000073 DAWHXS010000000
VERSION     DAWHXS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.073"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..297)
                     /locus_tag="WIE22_001463"
     CDS             complement(<1..297)
                     /locus_tag="WIE22_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4127533.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            485..1384
                     /gene="crgA"
                     /locus_tag="WIE22_001464"
     CDS             485..1384
                     /gene="crgA"
                     /locus_tag="WIE22_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ4127534.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1614..4829)
                     /gene="carB"
                     /locus_tag="WIE22_001465"
     CDS             complement(1614..4829)
                     /gene="carB"
                     /locus_tag="WIE22_001465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ4127535.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4869..5480)
                     /locus_tag="WIE22_001466"
     CDS             complement(4869..5480)
                     /locus_tag="WIE22_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127536.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(6328..6717)
                     /locus_tag="WIE22_001467"
     CDS             complement(6328..6717)
                     /locus_tag="WIE22_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ4127537.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7290..7415)
                     /locus_tag="WIE22_001468"
                     /pseudo
     CDS             complement(7290..7415)
                     /locus_tag="WIE22_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7461..8594)
                     /gene="carA"
                     /locus_tag="WIE22_001469"
     CDS             complement(7461..8594)
                     /gene="carA"
                     /locus_tag="WIE22_001469"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ4127538.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2041 a         2381 c         2447 g         2452 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgccc taaagggcgg ggttccaacc gaaaaggaaa
      481 cacgatgaaa accaattcag aagaactgac cgtatttgtt caagtggtgg aaagcggcag
      541 cttcagccgt gcggcggagc agttggcgat ggcaaattct gccgtaagcc gcatcgtcaa
      601 acggctggag gaaaagttgg gtgtgaacct gctcaaccgc accacgcggc aactcagtct
      661 gacggaagaa ggcgcgcaat atttccgccg cgcgcagaga atcctgcaag aaatggcagc
      721 ggcggaaacc gaaatgctgg cagtgcacga aataccgcaa ggcgtgttgc gcgtggattc
      781 cgcgatgccg atggtgctgc atctgctggc gccgctggca acaaaattca acgaacgcta
      841 tccgcatatc cgactttcgc tcgtttcttc cgaaggctat atcaatctga ttgaacgcaa
      901 agtcgatatt gccttacggg ccggagaatt ggacgattcc gggctgcgtg cacgccatct
      961 gtttgacagc cgcttccgcg taatcgccag tcctgaatac ctggcaaaac acggcacgcc
     1021 gcaatctaca gaagagcttg ccggccacca atgtttaggc ttcaccgaac ccggttctct
     1081 aaatacatgg gcggttttag atgcgcaggg aaatccctat aagatttcac cgcactttac
     1141 cgccagcagc ggtgaaatct tacgctcgtt gtgcctttca ggttgcggta ttgcttgctt
     1201 atcagatttt ttggttgaca acgacatcgc tgaaggaaag ttaattcccc tgctcgccga
     1261 acaaacctcc gataaaacac acccctttaa tgctgtttat tacagcgata aagccgtcaa
     1321 tctccgctta cgcgtatttt tggatttttt agtggaggaa ctgggaaaca atctctgtgg
     1381 ataattctat aaaaacctgc cataaaattg tttttactca cttaataact ttgagaaaaa
     1441 ataaataaga tgatgtatag ccgcggcaat ggcagtctat atggggagcg tgatagggct
     1501 gttccttatg tacggcttgt gtgtggcaac atcggctttc caaatgacaa aatgccgtct
     1561 gaaaccgtaa aaaaaaaaag gtttcagacg gcattttcaa cctgattcag gcatcagttg
     1621 cggtttttca aacgcccgtg cagttcttga acgctgtaca cgcccaagtg gtcgcggctt
     1681 ttcgcgcctt cgctcatcgc ttcgccgccg gcggtggtgg tgtattgcgg cacgcgttgc
     1741 tgcaatgcgc tttggcggat gatgtggctg tcggacacgg attgcggatc gctggaaacg
     1801 gtattcacga ccagtgcgat ttcaccgttt ttcagcgcgt cgccgatgtg cgggcggcct
     1861 tcggggactt tattgatggt ttggacaatc agcccgtgtt cggtcaggta ttgcgccgtg
     1921 ccgcgcgtgg cgcagatgcc gtagcctaag gcttggaagt ttttggcggt tttaatgacg
     1981 cgttctttgt cttcttcgcg cacggagagg aagattttgc cggtcgggtt gaggcgttcg
     2041 cctgcgccga gttgggcttt gtagtaggct tcgccgaagc ttgcgcccac gcccatgact
     2101 tcgccggtgg agcgcatttc cggacccaaa atcgtatcca cgcccgggaa tttgatgaag
     2161 gggaacacgg cttctttaac ggcatagaaa tcagggacga cttctttttc cacgccttgt
     2221 tctttcaggg aaatgcctgc catacagcgt gcgccgactt tggcgagcgg cacgccggtg
     2281 gctttggaga cgaaggggac ggtgcggctg gcgcgcgggt tcacttccaa tacgaacact
     2341 acgccgtcct gcacggcaaa ctgcacgttc atcagtccga ccacgcccag cgcgtacgcc
     2401 atcgctttgg tttggcggcg gatttcgtct tggatttctt cgcttaagga gtagggcggc
     2461 agcgagcagc cggagtcgcc ggagtggatg cccgcctgtt cgacgtgctg catgatgccg
     2521 ccgataacca catctttgcc gtctgaaacg cagtccacat cgacttcaat cgcgttgttc
     2581 aggaagaagt cgagcaacac ggggctgtct tcggaaacct gcacggcttc gcgcatatat
     2641 ttttgcaact gctcggcaga gtggacaatc tgcatcgcgc gtccgcccaa aacataagac
     2701 gggcgcacga ccagcggata accgatttct tcggctttga cgagtgcttc ttcttcgttg
     2761 tgggcgatgc ggttgggcgg ctggcgcagg cctaagtcgt tcaacacttt ttggaagcgt
     2821 tcgcggtctt cggcggcatc gatgctgtcg gcggacgtgc cgatgatgtt cacgccgttt
     2881 tcaaccagcg cgttggcgag tttgagcggg gtttgaccgc cgtaatgcac aatcacgccc
     2941 cacgggtttt cggtgcggac gatttccaac acgtcttcca atgtcagcgg ctcgaaatag
     3001 aggcggtcgc tggtgtcgaa gtcggtggac acggtttctg ggttgcagtt gaccataatc
     3061 gtttcaaaac cggattcgcg cagggcgagc gcggcgtgaa cgcagcagta gtcaaactcg
     3121 atgccctgac cgatgcggtt cgggccgccg ccgagaatca tcactttttt gcggtcggaa
     3181 ggacgcgctt cgcattcctc ttcgtaagtg gaatagaggt aggcggtttc ggtggcgaac
     3241 tcggcggcac aggtgtcgac gcgtttgtaa accggatgca gcttcagcgc gtagcggtgt
     3301 tcgcgaactt ctttttcgct tacgttcaac aattgtgcca aacgtttgtc ggagaagcct
     3361 ttgcgtttca gacggcgtag ggcggcgaaa tccaaatcac tcaaaatgcc gtctgaaacc
     3421 gctttttctt ccttcatcaa gtcttcgatt tgcgccagga accaagggtc gatggcgcag
     3481 atttcgtgga tttcttccag cgtgaagccg gcgcggaacg cgtctgccac aaacagcata
     3541 cgctcagggc cggggttcgc cagttcgcgg cggatttccg ctttgtcgga gcttctcgga
     3601 ttgaagccgc acaagccggt ttccaagccg cgcaaggctt tttggaagct ttcctgaatc
     3661 gtgcggccca tcgccatcac ttcacccacc gatttcatct gtgtggtcag gcggtcgtct
     3721 gcggcaggga atttttcaaa cgcgaaacgc gggattttgg ttacgacata gtcgatggac
     3781 ggctcgaacg acgcgggggt tttgccgccg gtgatgtcgt tgcgcaactc gtccagcgta
     3841 aagccgaccg ccagcttggc cgccaccttc gcaatcggga agcccgtcgc tttggaagcc
     3901 agcgcggacg aacggctcac gcgcgggttc atctcaatca caatcatctc gccgttttca
     3961 gggttcatcg caaactgcac gtttgagccg cccgtgtcca cgccgatttc gcgcaatacc
     4021 gccaacgaag cgttgcgcat gatttggtat tccttgtccg tcagcgtttg cgccggcgcg
     4081 accgtaatcg agtcgcccgt atgcacgccc atcgggtcga agttttcaat cgagcagatg
     4141 atgatgcagt tgtcgttctt gtcgcgcact acttccatct cgtactcttt ccagccgaga
     4201 acggactgct caatcagcag ctcgtgcgtg ggcgaggcat cgaaaccgcg ttcgcaaatc
     4261 gccaaaaact cgtctttatt gtaggcaatg ccgccgcccg aaccgcccat cgtgaaagaa
     4321 ggacgaatca gggtagggaa gccgacctgt tcctgcgccg ccaaagcttc gttcattgtg
     4381 tggcagacaa aagatttcgg gcaagacaaa ccgatttttt ccatcgcctc tttaaagcgg
     4441 ccgcggtctt ctgctttgtc gatggcgtct tccgtcgcgc cgattaattc aacattgtat
     4501 ttcgccagca cgccgttgcg cgccaaatcc agcgcacagt tcagcgcagt ttgaccgccc
     4561 atggtaggca gaatcgcatc gggccgctct ttggcaataa ttttttccac cgtctgccac
     4621 ataatcggct cgatgtaggt aacatccgcc atttcggggt cggtcataat cgtggcgggg
     4681 ttggaattca ccaaaatgac tttatagcct tcttcacgca aggctttgca ggcctgtgcg
     4741 cccgaatagt caaattcgca ggcctgaccg ataacgatag ggccggcgcc gatgataagg
     4801 atggatttta ggtcggtacg tttgggcatg atgggtcttt ctatgaaatc aaatgtttag
     4861 ctaaaaattt attgctgatt ttgattggct tttaattgct ctacgacagt attggcaatc
     4921 tcttttcctg atggggctgt aattgtgtaa taaaagctaa tactgcctat gaagaaactg
     4981 ataatggcta tgatataggc tatataactt tgtgttttca tttttttgtg atcatgtttg
     5041 atctgattta attcttttaa ttcagaaata attggagtta ataaatgttt ttgactttca
     5101 gatccatgcg tttccaaaag ttcatttatt cctattagtg ttggttttag ttcgtctgag
     5161 cagccatcaa aaggttcttc ttgttggatt tccttttgta actgtctaac taaatttaaa
     5221 tttaattcat cagtgtaata tagttgctta ataatttttc tggcagcaaa attattaagg
     5281 tttactgctt ctgtcatttg ttttatatca ttaagatcta ggttattgtg attatttttt
     5341 aatatatcaa ttattctaga tttaaatttc tttttcttta tattctctaa atatagctga
     5401 attggaataa aaacaattat aaagagcaaa agaaatatat atgttagtgc catagcccat
     5461 gtggcaatta tatttatcat ttatcaatat cttccatttg gtttatatat tgtctcataa
     5521 taagcaagcc gtagctcgca tgcagggtgt gtgcggtacg cacgcgttct ccattttccc
     5581 tgcaacccca acccaccgtg tgcgtgcctt gcggcacaca ccctaccgat gggttcaaag
     5641 gtcgtctgaa aaaacgctcg cggcgtttgt tccatctgcc ttgcgataaa ggctgcctga
     5701 aaaattcccg ctgttgatat ttcgggcatt ctttctcaaa ccgcctgcaa ccgcaaaatc
     5761 cgctgcaacc tgccccctcc tccgtggggg agggttgggg agagggcatt ccccgaattt
     5821 cggcaacctt tcccaacaac tcaaccaccg aaatacaagc cttgtggctt gttgccctct
     5881 ctctagctct ctcccacggg agagaggacg gagtggctgt agggctaagg gttctgcaag
     5941 gaaacaggtt gtgcaggttg ctttttgctt ttcagacgac ctgaaatcaa cggtaaatcc
     6001 aagtcgcttt tcaaaccgcc tgcaaccgca aaatctgctg aaacctactc cctcccccgt
     6061 gggggagggc tggggagagg gcattctccg aatttcggca acctttccta acactctaac
     6121 cgccaagaca caagccttgc ggcttgttgc cctctctcta gctctctccc acgggagaga
     6181 ggatggggtg gctgttgggg ctaaggtttc tgtaaactaa tcaggttatg caggctgctt
     6241 tttgtttttc agacggcctt tataaatgct caattcagtc ggctttatcc tattattttc
     6301 aataatccaa tcaaaatcag ccatctgcta tcgtgcagct tgcttttcca attcttgcaa
     6361 cacgagcagg atttctgtca gcacatcgtt tgtctgctgt aaaatctcgt gattccaaaa
     6421 ccgcagtacg gtaaagccca agctgttgag atatgccgtc cgcgcgtggt cgtatgcggc
     6481 ttgttctgtg tgctgccctc cgtctgcttc gacaatcaac ttgggcgtta cgcacataaa
     6541 atcaacaata tagtttccca tcggctgctg gcggcggaat ttatagccgt tcagacggcc
     6601 tgcccgcagg tgctgccaca attttgcttc cgcctcgctc atttcttggc gcatggcttt
     6661 ggcgcgttgg cgcaagatgg ggttgtcggt ggtcaagagt ttctcgtgcg ggttcatttt
     6721 gttgtcgtgc tgtttgctgc cctctctcta actctctccc acggggagag aggacggggc
     6781 ggttgttggg gttaaagggg ctgtaaggaa aacaagtcgt tcaggccact ttttactttt
     6841 cagacgacct gcaatcaacg gttgtaaatc caagttgctt ttcaaaccgc ctgtaaccgc
     6901 aaaacttgct gcaacctacc ccctcccccg tgggggaggg ctggggagag ggcattctcc
     6961 gaatcgcggc aatctttccc taattcccta actgcctcaa tacaagcctt tcggcttgtt
     7021 gccctctctc taacgctttc ccacgggaga gaggacggtg cggctgtagt gattaagggt
     7081 tttgcaagga aaacaaggaa aacaagttgt acaggctgtt ttttattttt caagcgacct
     7141 gaaatcaacg cttgtaaatc caagtcgctt ttcaaactgc ctgcaaccgc aaaatctgct
     7201 gcaacctacc ccctcccccg tgggggaggg ctggggagag ggcattttcc aagttgcggc
     7261 aatctttccc aaatccctta gtcttctaaa atacaagcct tacggttgtt gccaaatgaa
     7321 acttcttcca tacccaaaac ttgcgtgtaa aacgcaatgg ttcggtcaat gtcggcaacg
     7381 gtcagtacca gatggtcgag tgcgctaatc ttcatttccc gctttctttt tcaggcggcg
     7441 gcagtgccgt ctgaaagcca ttatgcccgt tttgccgctt tcatattgcc aatgaatttg
     7501 tcaaacaaat agccgacatc ttgcggaccg gggctggctt cggggtggcc ttggaagcag
     7561 aacacaggtt tgtcggtcag ctcgatgcct tgcaaagtgt tgtcaaacaa ggatttgtgg
     7621 gtaatgcgtg cgttagcggg cagggtgtcg gcatcaacgg caaaaccgtg gttttggctg
     7681 gtgatgacga ctttgccgct gtccaaatct tgcacagggt ggttcgcacc gtggtggctg
     7741 aagcgcattt tcagggtttt cgcgccgata gcgaggctga tgagctggtg tcccaagcag
     7801 atgccaaaaa tcggtttgcc gctttccatc agtttttgca cggcttcgat ggcgtaggtg
     7861 caaggctcgg ggtcgccggg gccgttggac aggaacacgc cgtcgggatt gagtgccaac
     7921 acgtcttgtg cgctcgtttg ggcggggacg acggtcaggc ggcagccgcg cgaggcgagc
     7981 atacgcagga tgttggtttt cacgccgaaa tcgtaggcga cgacgtggta aggctgttcg
     8041 tcaggggtaa cgaaaccctt gcccaatgcc cattcgcctt ccgtccattc gtaagtttcc
     8101 gtgcaggaaa cttcttttgc caagtctttg ccgaccatac tgccgaacgc ggcgatgagt
     8161 tcttgcgctt tttcgattgt ggcatccgca ccggtcagaa tcgcgccgcc ttgcgcgcct
     8221 ttttcgcgca acagcgtggt caggcggcgg gtatcgatgt cggcgatggc gacggttttg
     8281 ttgcgtacca gatagtcgtg caggctttcg gaggcgcgga agttgctgtg caagagcggc
     8341 aggtcgcgga taatcaggcc ggcggcataa acgctgcggc tttcttcatc ttcggcgttg
     8401 gtgccggtgt tgccgatgtg tgggtaggtg agggtaacga tttgtttgca gtaggacggg
     8461 tcggtcagga tttcctgata gccggtcatc gaagtattga acacgacttc gccggaagtc
     8521 gaaccttcgt aaccgattga tgtgccgtgg aatacgctgc cgtcagcgag gacgaggagg
     8581 gcgggggtgc tcatgatggg aatcctgttt ttaataaaat tcttgacaat gccgtctgaa
     8641 gggacttcgg cagggggcat ccggtcggaa aaatccggtc aaaaaaaaac acgccgcgca
     8701 agatagacgc gtaacgtgct tttcgggcaa gtgcctatac cctgaaagca gcatatttta
     8761 agacgaaaca tacccgattt caagcgcaaa cgcggcaggc gggctgatat gttcgcgcga
     8821 aaacggaaat ccgtggcgta aaaccgcccg ctccgcgccc actatgcccg ttacgcattt
     8881 ccccacgtcc gtccggcgaa actatgggaa tacccgaatc gtcattccca cgaaagtggg
     8941 aatccagttt tttgagtttc agtcatttcc gataaattgc cttagcattg aatgtctaga
     9001 ttcccgcctg cgcgggaatg acgcgtttca tttgccccca tcccgaaaac gcaaacaata
     9061 ccgtctgaaa gctctgtagg gagggcttca gcccaccgat tccatcaatc ccacatttcc
     9121 gccgttttca ccgaatccgc cccttttcac aatccagtcc tacgcaactg aaccgtcatt
     9181 cccgcgcagg cgggaatccg gacttgtcgg cacggaaact tatcgggtac aacggtttct
     9241 tgagatttta cgtcctagat tcccgcctgc gcgggaatga cggtatcggt ttgtcggcgt
     9301 ttcatttgcc cccctcccga a
//
LOCUS       DAWHXS010000074         9121 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWHXS010000074 DAWHXS010000000
VERSION     DAWHXS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.074"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(77..1333)
                     /locus_tag="WIE22_001470"
     CDS             complement(77..1333)
                     /locus_tag="WIE22_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ4127539.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLFDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1681..2022)
                     /locus_tag="WIE22_001471"
     CDS             complement(1681..2022)
                     /locus_tag="WIE22_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127540.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2225..3082)
                     /locus_tag="WIE22_001472"
     CDS             complement(2225..3082)
                     /locus_tag="WIE22_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ4127541.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFTNAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQGFHAFEPPLNPYGWRDVYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3143..3958)
                     /locus_tag="WIE22_001473"
     CDS             complement(3143..3958)
                     /locus_tag="WIE22_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ4127542.1"
                     /translation="MTTLKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4022..4192
                     /locus_tag="WIE22_001474"
     CDS             4022..4192
                     /locus_tag="WIE22_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127543.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCTSEPAVCHP"
     gene            4202..4987
                     /locus_tag="WIE22_001475"
     CDS             4202..4987
                     /locus_tag="WIE22_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ4127544.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDVVAANPKVLAQMLKIISAHV"
     gene            5257..7352
                     /locus_tag="WIE22_001476"
                     /pseudo
     CDS             5257..7352
                     /locus_tag="WIE22_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            7476..9026
                     /locus_tag="WIE22_001477"
     CDS             7476..9026
                     /locus_tag="WIE22_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ4127545.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLITAVKGEASGLFTHNPVLNIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     GMVSEPQQ"
BASE COUNT         2288 a         2163 c         2412 g         2258 t
ORIGIN      
        1 gttaatccac tataattgat gaaatgccgt ctgaacgctt tgaaaagggt tcagacggca
       61 ttttgtttaa acagggttat ttgcgttgca atacggcggc gaagaagccg tcggtttggt
      121 gtcgggcgga gttgaggcgc aggtatttgc cggtatccaa atcgattttc aaaccggcaa
      181 gcagttcggc gcagttgacg ggttcaaatt cgggatgttc ggacaggaaa cgttcgactt
      241 gcagctcgtt ttcttcgggc aggatgctgc aagtggcgta aaccaagcgg ccttgcggtt
      301 ttaccagttt ggaggcggca tcgaggatgc tgtgttgctg ttccaaaagg ttggccacgg
      361 tttcggcgga ttggcggtat ttgaggtcgg gattgcggcg taaagtaccc aaaccggagc
      421 agggcgcgtc caccaacaca cggtcggctt tgcctgccag tcgggcgata cggctgtcgt
      481 gttcgctgct gatgcgttcg gggtggatat tggtcagtcc ggcgcgggtc atacgcggtt
      541 tgaggttggc aaggcgtttt tcggcgatgt cgaaggcgta gattctgcct ttattggcca
      601 tttgcgcacc gacagccaag gttttgccgc cggcaccggc acagaaatcg acaatgattt
      661 cgccgcgttt tgcgcccacc aataaagcaa gcagctggct gccttcgtct tggacttcca
      721 gtgtgccgtc taaaaacagt tcgtgtttgt taagcgcgat tttgtttttc aggcggatgc
      781 cccaaggcga ataaggcgtt gcctctgcat cggcactttc ggcttgcaac agcggcagca
      841 ctttatcgcg tttgcctttc aaagtgttga cgcggatgtc gagcggggca ggctggttga
      901 tgctgcggcc gaaggcgagg atttcttctt cacgccaatg ctgttgcagt tgttcgacca
      961 gccattgcgg caattctgcg gcggtattca ggccgtctga aaactcggtt ttacgggctt
     1021 tcaaattgcc gaggaacgct gtttcttctt catcaaacag gtctttgatt tggctgatgt
     1081 tggtgcttct gccgagaacc agtgcggcga gtgcggcttt gcgcggctgt gcgtgcggac
     1141 ggcgcagggc agtgctgatt ttttgatagt ggcgcagcgc ggcaaaggcg gtttcggcga
     1201 tttcgtggcg gtcttggcgg ccgagctttt tatgttcgcg gaaataggcg gagaggacgg
     1261 catcggcagg ctgtttgaaa gtcagcattt cagccaaaac tttggcggta tggtcgagtt
     1321 gtgcggcgtt cataatgtaa tgggttctct tactgtgggt tattcataat caatctaacg
     1381 tccttaaaat aacaatagca tctttatcat tacttagtat tctatccgca ataagaaaga
     1441 tgtgttaaaa gtgcgcaatc gggatttgtc taaatttctc cgttaactac aaaggcagcc
     1501 tgaaaaaagt tctattttca ggtcgcttga acagcatccg atataatcac atcagcgatt
     1561 tcattttgta atcactcccc aaaacatgag gaaaaaaaca aaatttattt tcttcgttca
     1621 gtatatctat cctaaatatg ccatcactcc attgagtttt taatttgtat ttcgctcttg
     1681 ttaaatgttt ttctgtttgc cgatttcttt tgctaatgta aaaaatactc taccactctc
     1741 attggttaaa gttttcaagt attcatcaac atcagtaaca ttaataacaa ctgtttcatt
     1801 tgtatgttta ttaaattcta aatcaataat aatattccca ttaaccaata tttcatatat
     1861 atcttcttct tcagggagca tagacaaaca ataagcactt gaagctgaaa actctttttt
     1921 cagatactga agaagaaaag tatttttaat taaaatatta ttaaatttat ctaatgattg
     1981 cttgacttct ttagttgtcc aatttttaac attattcttc attgtattct acctgggtcg
     2041 tagttattaa attaccatac ttatttgttt ggatcgtcat aatattagtt ggcatgttgt
     2101 tgttatattt atcatatcca atatccttat taactcggat gactctttca taaattgtat
     2161 gggaaatgag atttccatca ctaagcctct caatagatat acgcgttaat gttgggaaag
     2221 ggtttcagac ggccttcata atgcgttcga tttcgtgcca aaagccgtcc ggccgacatt
     2281 ccaaaacggc aatcaagcct tggtcgatac ggcggatttc ggtttcgttc aatgtttcgg
     2341 tttcggcggc acgcgcaggt gcgaggtagg ccatgcggtc gagcagatgg tagcggactt
     2401 cttggcgttc aaacccgtat tccgcccaat cttgaatata gacgtcgcgc cagccgtatg
     2461 gattaagcgg cggctcaaaa gcatggaaac cttgcggaaa aaatccgatg ccgcgcacga
     2521 tggaaacggg ttttacgtca agcggcagac cgtgttgccg caaggttcgg atgccgtctg
     2581 aagtgtgcgg cagaccgagt tgggcggtca gtttggcggc ttttcccaac agcgtgtctt
     2641 tggggtcgag tccgcgcatg tcttcgagcc tgcccgtgcc gccgccgtaa tatttgcagg
     2701 tcagctcgat atggtagggc ttgccgttaa gccttaccac aaaatccgcc gcgccttgcg
     2761 tattgccgtc cgaaccggaa accgtgaggt tgtgcgcgag cagttcggcg tgtggcgcgt
     2821 tggtaaacca aaatgccagc agttcttcgg cgtaaatgcc gaggcggtgt ccgaacgggg
     2881 cgcgtcgggt gaggtgttcc gtcagcggcg cgggatcggc atccaatgcc aaaagatagc
     2941 ggaaaccgcg ttcccccaat agttcgcgca cgcccaattc gcaaccgctt tgccacaaag
     3001 gcggcgcagt cagcagcgag gcgaggtcgc ggacgggttg gctggtgagc ttccaccata
     3061 atgcgtctag ggcgtaattc atgcttatcc gaatatcagt catgccgtct gaaaagctgg
     3121 aaatgttttc agacggcctt aatcagtctt ttttatggat acgatggctg acggtattga
     3181 aaaaaccgcg caggatatcg atgtcttcgg tttgcgtgtt ggcgcgtccg aacaggctct
     3241 gcatacggcg catcagacgc tcgccgttgc ggcggttgaa aaagccgatg tcgttcatca
     3301 cgctttccat gtgggcgacc atgcctttga tttgctcgtg ggtcgcagcg tggtcttctt
     3361 gttgaagatg ggtcatgggc gaaccggttt ggctgaagat ttcgtagcac acgacctgca
     3421 cggcttgggc gaggttgagc gagaaatagt cgggattgcc gttgatggtc atcagtcggt
     3481 tgcaggcttg gacttcttcg atgctcaagc cgaaagtctc gttgccgaaa accagtgcca
     3541 ctttctcgcc tcggtttgcg gtctgcagta attcggatac caaatcgcgc ggggtttgca
     3601 gcggcgcagt aatttcgcgg cggcggctgg tcagggcgca ggcgatggtg gtgtcggcaa
     3661 gggcttcgtc caaagaagcg gcaatggtgg cattttccaa aacgtctgcc gcgccggaag
     3721 cgaggatgaa gctttcttcc ggtaatttaa acgattgagg atgctccggg tcaaacacgg
     3781 gcgggttttc cgtcatcggc gttgccatca gatttggggc gacgatggtc agtttgtgca
     3841 gacccattgt tttcatcgcg cgcgcggcag agccgatgtt ggcgggatgg ctggtgcgcg
     3901 tgaggataat gcggatgttg ccgagatagt cgggcagggc gggtttgaga gtagtcatgt
     3961 tcggttttcg gtcggaatgg aatagggtat aatgcgccgt ctttttcgtt cggacggtca
     4021 aatgccgtct gaaatgttct ttaacaacgt cggaaattta acgttgcgcc tgaagcagac
     4081 acgcgtcggg cggtatttta cccgatttgg caggatttac cccaaccaaa taaactccga
     4141 ttttgccgct ccgaaggact gcacgtccga accggcggta tgccaccctt aaggaaatct
     4201 gatgaatccg tttttgaata cagcctttaa agccgcccgc cgtgccggtc agatgatgat
     4261 acgcgccgcg ggcaatctcg atgccgtcaa aaccgacagc aaagcattca acgattttgt
     4321 ttccgatgtt gaccgcaatt ccgaaatcat cctggttgag gctttgaaag aagcctatcc
     4381 gcaccacaaa atcacttgcg aagaaagcgg ctcccacggt aaagccgcag ccgaatacga
     4441 atggattatc gatccgctcg acggcacgac caacttcctt cacggccatc cccaatacgc
     4501 catctctatg gcactcctgc acaaaggcgt gttgcaagaa gctttggtgt acgcccccga
     4561 acgcaacgac gtttacatgg cttcgcgcgg caaaggcgcg ttgctcaatg accgccgcat
     4621 ccgcgtttcc aaccgcatcg aattgaaccg ctgcctgatc ggtaccggct ttcctgttgt
     4681 cgatcaaagc atgatggaca agtatctggc gattttgaaa gatttcttgg caaaaaccgc
     4741 cggcggccgt cgtgaaggcg cggcttcttt ggatttgtgt gctgtggcga ctggtcgttt
     4801 tgacggcttt ttcgagttca acctcaaacc ttgggatatt gccgcaggcg cattgattgt
     4861 tcaggaagcg ggcggtatcg taaccgatat gacgggtgaa gacggctggc tggaaagcgg
     4921 cgatgttgtc gctgccaatc ccaaagtact ggcgcaaatg ttgaaaatca tttccgcaca
     4981 cgtttaaatt tcagcccgct cccgggaatg cgggaaaacg ggcgggtatg ccgtctgaag
     5041 tatcgcgccg atatttcaga cggcattttt actggcagcg ggggcaggaa ggtggaaacg
     5101 gctaaatgcc cgaaactgcc gaagatggaa acccatatct gattaaacgg agaaccctgt
     5161 acgatttgtt tatagtggat taacaaaaac cagtacggtg ttgcctcgcc ttagctcaaa
     5221 gagaacgatt ctctaaggtg ctgaagcacc aagtgaatgg gcaaagccaa cgcctttacc
     5281 gcaccgatta ccgtcgtcaa ttacgacgaa caagccctca ataacaccga agcgcgtact
     5341 ttggtggatg ccgtagcgaa aaaagacgct tccgtttggc agttcggcgg cgaaagcaac
     5401 acgctgaccg gtctgtattt cagaggttat cagcttgact cgcgccaatt cagcgtcaac
     5461 ggtttggcag gtatgtacgg cacgcaaggc acggccagcg tgcaagtcgg ctcggcacaa
     5521 ctgattaaag gcgcgtccac cgccgtaaac ggcatggacc ctgaaggcgc ggtatccggt
     5581 tccgtcaata ttgaaaccaa aaaagccgcc gacgaagaca accgcaaaat cggtttgggc
     5641 tggtttagca acaaccgcgc ccaaggtacg ttcgacttgg gtcaacgctt cggcgaaaac
     5701 aaagaattcg gcgtgcgcgc caacggcaaa ctgcgccatg gcgacacccc gcgccacggt
     5761 tacagcgaag acaacaaaga atttgcattg aatgctgatt atcgcggcga aaaagtgcgc
     5821 gtggcgttcg actccatcta cgcgaaacgc aaaaccaacg gcggccgcgc gcgtatgcag
     5881 gatattcaaa acgccggcgg ccgcttgttt gacgcgccag acggcaaaat caacctgctg
     5941 ctttcgtgga actggcaaaa caccgtcggc gaaaccaata tgctgacctt tgaatgggat
     6001 gcgtttgaca atgcccaaat taccggcggt atcggctaca acaaggcgcg ttattacggc
     6061 acactgattt ccccgaccgt ttgcggtaca agcggtgcga gcgaccaaac cgctacctgc
     6121 acaagtgcca agcaatacca caccggcacg gcgagcctga ccgaccaata tttccgcaca
     6181 ttgagtatga acctgaccgc acgcggcgaa tttgaaaccg gcccggtaac acataactgg
     6241 agtatggctt tcgaccgcgt tatccgccag cggaaaacca taaacggcag cgggaacggc
     6301 aacagcaaga tagaagtgaa ggcaaacgaa aatattgcga atcaattggc atcgtttaca
     6361 gccgactatc ccaattcttg ggagaactct gccaatttgg atgccaatat caaagtcaac
     6421 agcctggctt tgtccgacac cttgggcttc gtggataaca aataccgcct gactttgggc
     6481 ggacgtttcc aaacagtcga atataccgac aaaaaatcac agagcggcga tgccaaacgt
     6541 ttcagcccga tgctgatggc ggcgtgggtg ccgcaacctg atttggttgt gtacggcaac
     6601 tatatggaag acttggagcc tgccgacatc aaaaccgatg attccggcga aacgacaatg
     6661 gcgaaaccgc gtgtcagccg ccagtttgaa gtgggcgtgc gtaaaaactg gggcaatttc
     6721 gtaaccacgt taaacgcgtt ccaaatcaaa cgcccggcta ctggcgcggt catacaacta
     6781 ccaagacaac aaaaggggta acaacaacgc tgacctatgg caacaattcc gatttcgccc
     6841 gctataaggc gcaaggcggc gtagccggcg acgagcaagg tatggaacgc aatcgcggta
     6901 tcgagttcaa tgcctatgct aacctgttaa acaaaaccct gcgtccgact ttgggcctga
     6961 tgtatctgca atcgaccgtg aaagactacc caaattcacg cgatatgctg gttaacggcg
     7021 tacaagtcgc caatccgcgc gtaattgcca aggcaggttt ggaatgggat acaccgtttg
     7081 ccaaaggctt gactttaaac agcaatgttt cgtatttcgg caagtcttac caagacacgc
     7141 aaaaacaata cgccttccca tcctatacct tggttgatgt aggcgcgcgc tacaaaacca
     7201 agctcggcaa aaatacgttg accgtcagca gctcggtgga aaatctgttc aacaaaaact
     7261 actggcaggt acagcgcggc caatacgacc gcagcttcgc cgtcgtcggt ctgccgcgta
     7321 cttactggct gaaggcggaa ttggatttct gatttctccg catctggaaa atgccgtccg
     7381 aacaggtttc agacggcatt tcttttaaag agatatggcg ggatacgctt aaataatgct
     7441 attctagcgt ctttgaaacc tgcttgtttg aagtgatgaa aaaacctttg atttcggttg
     7501 cggcagcatt gctcggcgtt gctttgggca cgccttatta tttgggtgtc aaagccgaag
     7561 aaagcttgac gcagcagcaa aaaatattgc aggaaacggg cttcttgacc gtcgaatcgc
     7621 accaatatga gcgcggctgg tttacctcta tggaaacgac ggtcatccgt ctgaaacccg
     7681 agttgctgaa taatgcccga aaatacctgc cggataacct gaaaacagtg ttggaacagc
     7741 cggttacgct ggttaaccat atcacgcacg gcccttttgc cggcggattt ggcacgcagg
     7801 cgcacattga aaccgagttc aaatacgcgc ctgaaacgga aaaagttttg gaacgctttt
     7861 ttgggaaaca agctccggtt tcccttgcca ataccgttta ttttaacggc agcggtaaaa
     7921 tggaagtcag tgttcccgcc ttcgattatg aagagctgtc gggcatcagg ctgcattggg
     7981 aaggattgac gggaaaaacg gtttatcaaa aaggtttcaa aagctaccgc aacagctatg
     8041 atgcgccctt gttcaaaatc aagctggcag acaaaggcga tgccgcgttt gaaaaagcgc
     8101 atttcgattc ggaaacttca gacggtatca atccgcttgc tttgggcagc agtaatctga
     8161 ccttggaaaa attctcctta gaatggaaag agggtgtcga ttacaacgtc aaattgaacg
     8221 aactggtcaa tctcgttacc gatttgcaga ttggcgcgtt tatcaatccc aacggcagca
     8281 tcgcaccttc caaaatcgaa gtcggcaaac tggctttttc aaccaagacc ggggaatcag
     8341 gatcatttat caacagtgaa gggcagttcc gttttgacac actggtttac ggcgatgaaa
     8401 aatatggccc gctggatatc catatcgctg ccgaacatct cgatgcttct gccttaaccg
     8461 tattgaaacg caagtttgca caaatttctg ccaaaaaaat gactgaagaa caaatccgca
     8521 atgatttgat tactgcagtc aaaggcgagg cttccggatt atttacccat aacccggtgc
     8581 taaatatcaa aactttccgt ttcacgctgc cgtcgggaaa aatcgatgtg ggcggaaaaa
     8641 tcatgtttaa agacatgaag aaggaagatt tgaaccaatt gggactgatg ttgaagaaaa
     8701 ccgaggcaga catcagaatg agtattcctc aaaaaatgtt ggaagatttg gcggtaagtc
     8761 aggccggaaa tattttcagt gtaaatgccg aagatgaggc ggaagccaga gcaagcattg
     8821 cagatattaa tgaaacattg cgcctgatgg tggacagtac ggttcaaagt atggcaaggg
     8881 aaaaatatct tactttagac ggtaatcaga ttgatacggt tatttccctt aaaaacaacg
     8941 ccctgaagtt aaacgggaaa acgctgcaaa atgaacctga tcccgatttt gatgagggcg
     9001 gtatggtttc agagccgcag cagtaatggg tcaaatgccg tctgaaatgt tcaggcggca
     9061 ttttgttgta ttggaaactg ccgttttcaa ccattccttt agataagccg tgtcggattc
     9121 g
//
LOCUS       DAWHXS010000075         9072 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWHXS010000075 DAWHXS010000000
VERSION     DAWHXS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.075"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(129..464)
                     /locus_tag="WIE22_001478"
     CDS             complement(129..464)
                     /locus_tag="WIE22_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ4127546.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            593..1498
                     /gene="mtrA"
                     /locus_tag="WIE22_001479"
     CDS             593..1498
                     /gene="mtrA"
                     /locus_tag="WIE22_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ4127547.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1560..2048)
                     /locus_tag="WIE22_001480"
     CDS             complement(1560..2048)
                     /locus_tag="WIE22_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ4127548.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2195..3097
                     /locus_tag="WIE22_001481"
     CDS             2195..3097
                     /locus_tag="WIE22_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ4127549.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLAEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3138..4268)
                     /locus_tag="WIE22_001482"
     CDS             complement(3138..4268)
                     /locus_tag="WIE22_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4127550.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4473..7097
                     /gene="alaS"
                     /locus_tag="WIE22_001483"
     CDS             4473..7097
                     /gene="alaS"
                     /locus_tag="WIE22_001483"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ4127551.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELAKAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7159..7890
                     /locus_tag="WIE22_001484"
     CDS             7159..7890
                     /locus_tag="WIE22_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127552.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8197..>9072
                     /locus_tag="WIE22_001485"
     CDS             8197..>9072
                     /locus_tag="WIE22_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ4127553.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYITRGVFDFMFGIEAKVRQI
                     QEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRN"
BASE COUNT         2308 a         2382 c         2189 g         2193 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aaaaaacggc tgtatatgtt gtctgcaaaa
       61 tatcgggaag cataacagac aaaatgccgt ctgaaatatt ttcagacggc atttcaatcc
      121 gattcggatt atttttgcgt ttcaaccgct tccaatgcac gcagggcgta agtgtaagcg
      181 gcaccggcat tcagggcgat ggcggttgcc aatgcgccgg cgatttcgct gtcggtcgca
      241 ccggctttgg cggcggcggc ggcgtgaaca ctgatgcagc tctcacaacg tgtggtaatg
      301 gcaacggcga tggcaatcag ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc
      361 tgttccaatg cgccgtaggc ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac
      421 gattttttaa ccaatgcggt atgttctttc caatctttaa acattttctt ttcctttctc
      481 ttgcgtttaa ccctgatacg cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt
      541 attcagttgt gtttctcgtt taatcatctc attttatggt tcaaaaagat ttatggacat
      601 tctggacaaa ctggtcgatt tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt
      661 gggcggacaa tggtcggtac ggcatgaaac cttgcaacgc gaaggattgg tacacattgt
      721 tacatcgggc agcggctatc tctgcatcga cggcgaaact tccccgcgtc cggtcagtac
      781 aggggatatt gtatttttcc cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg
      841 cggagaaagt ttacaaccgg atatgcggca gcacggtgcg tttacggtca aacagtgcgg
      901 caacggacag gatatgagcc tgttttgcgc ccgtttccgc tacgacaccc acgccgattt
      961 gatgaacggg ctgcctgaaa ccgtttttct gaacattgcc catccgagtt tacagtatgt
     1021 ggtttcaatg ctgcaactgg aaagcaaaaa acctttgacg gggacggttt ccgtggtcaa
     1081 cgcattgtcg tccgtcctgc tggtgcttat cctgcgcgcc tatctcgaac aggataagga
     1141 tgtcgaactc tcgggcgtat tgaaaggttg gcaggacaaa cgtttgggac atttaatcca
     1201 aaaggtgata gacaaaccgg aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa
     1261 tatgtcgcgc gcgcaactga tgcgccgttt caaaagccgg gtcggactca gcccgcacgc
     1321 ctttgtgaac catatccgcc tgcaaaaagg cgcgttgctg ctgaaaaaaa acccggattc
     1381 ggttttgtcg gtcgcactgt cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt
     1441 caaacggcaa tatcacgttt cgccgggtca ataccggaaa gaaggcgggc aaaaataaat
     1501 cggggcttca aacgcaaatg ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt
     1561 catttcaagg gctgcatctt catcacttcc atcaaaaagt tggtaaatgc ggggttgttg
     1621 ggtttgacat ccatattttt ccaacgctgc tgccagccgc gcaaggcatt ctggatatac
     1681 agcttggact gttccgtatt gattgcgccc cgctggctgt ctatcgccga acgcaggtag
     1741 atttcataca tactgtcatc gacggcattg cgtccgacca ggcgttttct gaagttgttc
     1801 agatattgcg ccgcctgaac cttggtcatt ttaccgatac ccacctgata gcccaagcgc
     1861 gtcgcttcat cgctgatttt ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct
     1921 tttgcaggtg cttccgtttt gacggtattg ataggattca cggggatttc cgtcaatgtg
     1981 ggcacataaa tagactggca gccggaaaga actgccacaa tggaaagagg gataaggtat
     2041 tttttcatgc ccccattata atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa
     2101 aataaaattt cggcatcaga agcaggcaaa aacacattcc acaagccttg ccgcaaggtt
     2161 tacaatccga ccgtccttat cgcaacgacc gtttatggat accgcaaaaa aagacatttt
     2221 aggatcgggc tggatgctgg tggcggcggc ctgctttacc attatgaacg tattgattaa
     2281 agaggcatcg gcaaaatttg ccctcggcag cggcgaattg gtcttttggc gcatgctgtt
     2341 ttcaaccgtt gcgctcgggg ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca
     2401 ttggaaaaac cacttaaacc gcagtatggt cgggacgggg gcgatgctgc tgctgtttta
     2461 cgcggtaacg catctgcctt tggccaccgg cgttaccctg agttacacct cgtcgatttt
     2521 tttggcggta ttttccttcc tgattttgaa agaacggatt tccgtttaca cgcaggcggt
     2581 gctgctcctt ggttttgccg gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca
     2641 ggaaacggcg gcactcgccg ggctggcggg cggcgcgatg tccggctggg cgtatttgaa
     2701 agtgcgcgaa ctgtctttgg cgggcgaacc cggctggcgc gtcgtgtttt acctttccgt
     2761 gacaggtgtg gcgatgtcgt cggtttgggc gacgctgacc ggctggcaca ccctgtcctt
     2821 tccatcggca gtttatctgt cgtgcatcgg cgtgtccgcg ctgattgccc aactgtcgat
     2881 gacgcgcgcc tacaaagtcg gcgacaaatt cacggttgcc tcgctttcct atatgaccgt
     2941 cgttttttcc gctctgtctg ccgcattttt tctggccgaa gagcttttct ggcaggaaat
     3001 actcggtatg tgcatcatca tcctcagcgg tattttgagc agcatccgcc ccactgcctt
     3061 caaacagcgg ctgcaatccc tgttccgcca aagataaaaa atgccgtccg aacatccttc
     3121 agacggcata tcgggcttta tttccccgcc ttcacatcct gccactggcg caccataaac
     3181 ttcaatgccg ccggctggat aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt
     3241 gggaaaatcg tattgtcgtt tttaaattcg tcttccatca gctcacgcgc aggcttgctc
     3301 gaaggcgcgt aagtaacgaa attgccgttt ttcgccgaca cttccgggtc gaggaagtcg
     3361 ttgatgtatt tgtgcgcgtt ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc
     3421 acccaaatcc ccacgccctc tttgggcatc atcacgcgga ttttttcctt gccgcccgct
     3481 tcttcggcac ggcgtttggc gatgttcaaa tcgccgccga aaccgattgt tacgcaggta
     3541 tcgccgcgcg ccaaatcatc gataaagccg gacgaagtaa agcgtttgat attggggcgg
     3601 tttttcttga gtagggcggt tgcctccctg atgtcttccg tattgctgct gttcgggttt
     3661 ttacccaaat agttcaacac cataggatag atttccgccg cgctgtccaa atagctgatg
     3721 ccgcattgct tgagtttgga cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc
     3781 ggcagcttgt ccgtacccaa agcctttttc acgcgttcgg tattgatggc gaaggtattt
     3841 gtcccccaat aaaacggcac ggcgtattcg tggccgggat cgaccccgtc catcagcctc
     3901 atcatttcgg ggttgaggtg tttataattg ggaatcagcg acttatcgat tttctgatac
     3961 gcacctgcct taatctgcct gcccacaaac gcattggacg gcgcgacaat gtcgtaacca
     4021 gattttccgg tcagcacctt gctttccagc gtttcatcgc tgtcgtacac atcataagta
     4081 accttgatgc cgtttttctt ttcaaaatcg gcaacggttt ccggatcgac gtattccgac
     4141 cagttgtaaa ttttcaatac gtttcggttt tccgccggcg ccggtttttc ggcaggcggt
     4201 ttgtccgaac cgccgcacgc tgcaagcagc aaagcagtca ggacggccag gggcagatgt
     4261 ttggtcatta tcattccttg catatcgggt tggagaaagc ggccattata gccgatattg
     4321 gcaacagggc ttcagacggc attcaaaatc ccgccacact cttccgaaaa ccgccgcttc
     4381 catagctaga aacagggatt tgcggtaaga taccgccgtt cgttttccct gcttttacca
     4441 tgacaagaca tttgagagac attgaaaaaa ttatgaaaac ctccgaactg cgccaaaaat
     4501 tcctaaaatt ttttgaaacc aaaggccaca ccgtcgtccg ctcttccagc ctcgtgccgc
     4561 acgacgaccc gaccctgctg tttaccaacg cgggcatgaa ccagtttaaa gacgtattct
     4621 taggtttcga caaacgcccg tacagccgcg ccaccaccgc gcaaaaatgc gtacgcgcag
     4681 gcggcaagca caacgacttg gaaaacgtcg gctacaccgc ccgccaccac accttctttg
     4741 aaatgatggg caacttctcc ttcggcgact acttcaaacg cgacgccatc cacttcgctt
     4801 gggaatttct gacttccccc gaatggctca acatccctaa agacaaactg ttggcgaccg
     4861 tttacgcgga agacgacgaa gcctacaaca tctggttgaa cgaaatcggt atgccgtccg
     4921 agcgcatcgt ccgcatcggc gacaacaaag gcgcgaaata cgtgtccgac aacttctggc
     4981 aaatgggcga caccggccct tgcggcccct gctccgaaat tttctacgac cacggcgaag
     5041 aaatctgggg cggcattccc ggcagccccg aagaagacgg cgaccgctgg atcgaaattt
     5101 ggaactgcgt atttatgcag ttcaaccgcg acgaacaagg caatatgaat ccgctgccca
     5161 agccgtccgt cgataccggt atgggcttgg aacgtatggc cgccgtcatg cagcatgttc
     5221 acagcaacta cgaaatcgac ttgttccaag acctgctcaa agccgttgcc cgcgaaaccg
     5281 gcgcagcttt cagcatggat gaacccagcc tgaaagtcat cgccgaccac atccgctcct
     5341 gctcgttctt gattgcagac ggcgtgttgc cttccaacga aggccgcggc tacgtattgc
     5401 gccgcatcat ccgccgcgcc gtgcgccacg gttacaaact gggtcaaagc aaaccgttct
     5461 tccacaaact ggtggccgat ttggtcaaag agatgggcga tgcctaccct gaattgaaag
     5521 aaaaacaagt tcaaatcgaa gaagcgttga aaaacgaaga aagccgtttt gcccaaacgc
     5581 tggaaaccgg tatggctttg ttggaaaacg cgttggctaa aggcggcaaa acactcgacg
     5641 gcgaaatcat cttcaaactc tacgatactt acggtttccc atacgatttg accgccgaca
     5701 tctgccgcga acgcaatatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc
     5761 aacgcgcacg cgcacgcgcc gcccaaagct tcaaagccaa cgcccaactg ccttatgacg
     5821 gtcaagacac cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctcgccc
     5881 tctacaaaga cggcgagcaa gtcgacgaat tgaacgaagg cgacagcggc gcagtcgtca
     5941 tcgactttac cccgttctat gcagaatccg gcggccaagt cggcgacgtc ggctatatct
     6001 tctcaggcga aaaccgcttt gaagtacgcg atacccaaaa aatcaaagca gccgtattcg
     6061 gccaattcgg tgtacaaact tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag
     6121 tggacgacga aatccgcaat gccaatatgc gcaaccacag cgcgacccac ctgatgcaca
     6181 aagccctgcg cgacgtattg ggcgaacacg tcgaacaaaa aggctctttg gttaccgccg
     6241 aatccacccg tttcgacatt tcccatcccc aagcggtaac tgccgaagaa attgccgaag
     6301 tcgaacgccg cgtcaacgaa gccattttgg cgaacgttgc cgtcaatgca gccattatga
     6361 gcatggaaga cgcgcaaaaa accggcgcga tgatgctttt cggcgaaaaa tacggcgacg
     6421 aagtgcgcgt actgcaaatg ggcggtttct caaccgaatt gtgcggcggc acacacgttt
     6481 cacgcaccgg cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggcg
     6541 tgcgccgtat cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc
     6601 gtttggtgaa agacatcatt gctgaaacca aagcccaaac cgaaaaagac gtactggcga
     6661 aaatccaagc aggcgcggca cacgccaaag ccttggaaaa agagttggca aaagccaaag
     6721 ccgaactcgc cgtccacgca ggcgcaaaac tcttggacga cgcaaaagac ttgggcgcag
     6781 ccaaactcgt tgctgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgttaccg
     6841 atttaaccgg taaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag
     6901 tctccctgtg cgccggcgta tccaaaccgt tgaccggcaa agtgaaagca ggcgatctgg
     6961 ttaaatttgc agccgaacaa gtcggcggca aaggaggcgg ccgtcccgac ttggcgcagg
     7021 ccggcggcac ggatgccggc aaattgcccg aaatgctggt aagcgtggaa agctggcttt
     7081 gtcagaaatt aagttaaatt taaatcttaa taagccattt gagtcttgtt caaatggctt
     7141 atattttgga ttgttagaat gaatactaaa acagagttac aaaaactttt agaagaggat
     7201 atatcaactt taacagaaac actaatttgt gcagatgctc ttcctccaag atatgtacgt
     7261 agcattgcaa ctcctattgt ccgtcgatgg ttaattgaca aacaattaaa tattctcgca
     7321 aaagaaatag gtttaactat agaattgccc atccttgata caagtttggt atttgaaaaa
     7381 ttgtcaacct tagagaataa agttaatttt tatatggctg gtggagttta tttaggcgga
     7441 gaatttatta gctctatata tcattcatca caagaatttt ctggtgaacc aataatctat
     7501 gctgaaccaa atataatttt atgtcctgct gaaaaatttt taactttaaa acgagttttc
     7561 cataatggaa atatttttaa tatgaatcag ataattactt ttttatccaa taaacaagga
     7621 ggagtacatt ttgataaaaa ttacgataaa tataaaactt ggcaagttgc tatagaaaaa
     7681 gcagcaaatt ttttaaaact cggtaatcca tataatgaag ataaattatc tcttagtgaa
     7741 gaacatgata ctattttagt agttttacca ttagaaaaag gatatgaatg gaattgtctt
     7801 gagatagaag tactatccgc tgctcaatct ctagcaaata tctattgcaa caaagttcgc
     7861 ctcatagatg gtcatgtttg gaaggaataa aagaaaaagc atagttggta atttttagtt
     7921 acgtagggtg ggcttcagcc caccgttttt tcatttttga ttgttgattg gtgggctaaa
     7981 gcccacccta cggttactaa aatcccgtca ttacctcgca ggcgggaatc cggtttgttc
     8041 ggtttcggtt ttttagggac agtgccgcaa cattaaattt ccagattccc actttcgcac
     8101 aaataacagc gcaaataggg caaagataag gatatgggtt tttttctttg aatttactat
     8161 acaattcgcc cgtccccgtt atagaaaccc aagccgatga aaaccctgct ccaactccaa
     8221 accgccgcac aaaacttcgc cgcctactac aaagaccaaa ccgacgaacg ccgcgagaaa
     8281 gacaccttct ggaacgaatt tttcgccatt ttcggcatcg accgcaaaaa cgtcgcccac
     8341 ttcgaatacc ccgtcaaaga ccctgccgac aacacccaat tcgtcgatat attttgggag
     8401 ggcatcttcc ttgccgaaca caaatccgcc aacaaaaacc tgaccaaggc caaagagcag
     8461 gcggagcgtt atttgcagga aatcgggcgc accaagccct ctgcgctgcc cgaatattac
     8521 gccgtcagcg attttgccca tttccacctt tatcgccgcg tacctgaaga aggcgtggaa
     8581 aaccaatggc agttcccttt ggaagcattg cccgaataca tcacgcgcgg cgttttcgac
     8641 ttcatgttcg gcatcgaagc caaagtccgc caaattcaag aagaagccga tattcaagcg
     8701 gcggcggcca tcggcaggct gcacgacgcg ctcaaagaag aaggcattta cgaagaacac
     8761 gagctgcgcc tcttcatcac gcgcctgctt ttcctctttt ttgccgacga cagcgccgtt
     8821 ttccagcgca actatctctt tcaagacttt ttagaaagct gcaaagaaac cgacacgctc
     8881 ggcgacaagc tcaaccaact ctttgaattc ctcaacacac ccgaccaaaa gcgcagcaag
     8941 acccaaagcg aaaaatttaa aggtttcgaa tacgtcaacg gcggcctgtt taaagaacgc
     9001 ctgcgcacct tcgacttcac cgccaagcag caccgcgcct taatcgactg cggcaatttc
     9061 gactggcgca ac
//
LOCUS       DAWHXS010000076         9070 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWHXS010000076 DAWHXS010000000
VERSION     DAWHXS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.076"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..238)
                     /locus_tag="WIE22_001486"
     CDS             complement(<1..238)
                     /locus_tag="WIE22_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ4127554.1"
                     /translation="MGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGRILDNS
                     LKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIK"
     gene            complement(279..875)
                     /locus_tag="WIE22_001487"
     CDS             complement(279..875)
                     /locus_tag="WIE22_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ4127555.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGDS"
     gene            complement(1041..2051)
                     /locus_tag="WIE22_001488"
     CDS             complement(1041..2051)
                     /locus_tag="WIE22_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ4127556.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(2048..3592)
                     /locus_tag="WIE22_001489"
     CDS             complement(2048..3592)
                     /locus_tag="WIE22_001489"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ4127557.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(3665..4669)
                     /gene="rfaD"
                     /locus_tag="WIE22_001490"
     CDS             complement(3665..4669)
                     /gene="rfaD"
                     /locus_tag="WIE22_001490"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ4127558.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(4798..5829)
                     /locus_tag="WIE22_001491"
     CDS             complement(4798..5829)
                     /locus_tag="WIE22_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4127559.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(5831..7066)
                     /gene="dcm"
                     /locus_tag="WIE22_001492"
     CDS             complement(5831..7066)
                     /gene="dcm"
                     /locus_tag="WIE22_001492"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ4127560.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(7103..8074)
                     /gene="hldA"
                     /locus_tag="WIE22_001493"
     CDS             complement(7103..8074)
                     /gene="hldA"
                     /locus_tag="WIE22_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ4127561.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(8129..8869)
                     /gene="pyrF"
                     /locus_tag="WIE22_001494"
     CDS             complement(8129..8869)
                     /gene="pyrF"
                     /locus_tag="WIE22_001494"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ4127562.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
BASE COUNT         2132 a         2020 c         2149 g         2769 t
ORIGIN      
        1 cttttattaa cgcatgctct cctatggttg ccgttgtcga catcattatt gatttagcag
       61 gaaacagctt gccgccctta actgcgcttt ttgatatatg cttcaatgaa ttatctaaaa
      121 tacggccatt ttccctaata tcttccattc taaaccaagg gattgagcca ttttcccaat
      181 attctttatt tgatttggaa ggggtatagc cattcttcaa gtcaaacacc tcccccaacg
      241 tcttccaaac cacatctttc agacggcctt tatagccatc agctatcccc cccgatttga
      301 ttgtcaaaat ctaaaagaaa gtcgcggtaa taccggtatt ggcgtttgcg cagggctaat
      361 tcagcttcca gctcggtgaa tttgtcaagt atttttacaa ttttttgttg ggtttccagg
      421 ggtgggatag ggattaataa ttttttcaag ttactggcat ttagagcagg aatacctgta
      481 aatgtacata aggcatgaat ctcttgttca aattcaagta atatatgata gagaaaacga
      541 acatccaatt ctgttctatt cttaatagta actgcataat ttaagttgcc acgaaaatat
      601 cttccttctt gccacgttat tgagccgact cccgctcctc ttgttgtgat gccgataggg
      661 tcattttcag tgttccattc atcaatatag ccaagcggct ctttgccact gttaataact
      721 ggataactac caatattatt tgagattttc tgcttactta cagcttgtcc tgtttttatg
      781 atagctatcc cattttctcc acccaacggc ttccactcca ccggtgccgt ctgaatcatc
      841 tcaatcaatt ttttcgcttt gctttgcata tccatggttg ttttccgttt ctttcccgct
      901 gtggcgggat gtggggcggt tgtggggtgc ttcggctcac tctgtccgtt tgccgttatt
      961 tctgcgggct gaagtccgtc ctgctttcag gctgcctgca atggtgtgca ggctgctttt
     1021 tcaggtagcc tgaatggtag ttattttgtg cggtcttttt tctgtttgat ctgttgcacg
     1081 gctgtttttt ccaacgcttt aacgctttcc agataggctt gctccaccgg cgacaggatg
     1141 cgcgcttcat aggcgcggta ttcgcgttcg gctttctgct ctgcctgttt gcggctgatg
     1201 ctgcctgcac cctgtaatgt gccttgtccg tacagcccgg aaaatttgtc caattcggct
     1261 atccagtcgc gcatatacat ggggctttgc tcctgcgctt tgatttccgc taggtcgaag
     1321 aaggcggaaa ccagacggtt caggcggaac agttcgtctt cggtcagata gtttttggcg
     1381 attttggctt cattcagcgt ggggattgcg ccttgaaagg tggtcagccc cataaagtct
     1441 ttgctgctgt cggcacggct gtatatcagc tcagctgcgg tttgccggct ggcggcatag
     1501 tgcagtttgt tttgaacggc ggcaaaaaag gtttggcttt cgctgctttt ggggttgtag
     1561 tcttggctgg tggcatataa atcaagcact tgccggtata gggctttttc gctgctgcgg
     1621 atgtcgcgaa tgcggttgag cagttctttc caatagtcgc cgccgcctgt gcctttcagg
     1681 cgttcgtcgt ctatggcaaa gcctttggtc agatattcgt ccagccgttc ggttgcccat
     1741 tggcggaatt ggatgccccg cgcggaacgg acgcggtagc cgacggcaat aatcatgggc
     1801 agggaataat gggcgatttt gcggtttacc ttgcgcccgt tttcattttg aactgtcaac
     1861 tggaagttga cagttgcctt ctcttccaat tcttgctctg caagaatggt tttaatgtgt
     1921 ttgctgatat tttgtttggt ggtttggtac agttctgcca tgtccgcctg cgtcagccaa
     1981 agctgtccgc caaattcctg caaggcaaat tgggcagtgc cgtcgttggc ggtgtatagg
     2041 atgatgctca tgcttcaatc tctgcaatca cttcgtcaat ttcacgccgc agccgttcga
     2101 ttttggcaac ggtttcgctg atttcggcgt taagctgttt gatgtcgata atttcgcggg
     2161 tgtcttcggg ttcaacatag ctgctgacgg cgaggttgta gccgttgtct ttgacggttt
     2221 gctgggcggc gttttgggcg atatgcggca catcggcttt atcggcgaag agtttgacga
     2281 tttcggcaat gtgttcttcg gttaagacgt tgttgttggt ttctttttta aagaagccgc
     2341 ctgcgtcgat gaattggatg tcggtattgt ctttgtgttt ggacaaaacc agtatattga
     2401 cggcgatgcc ggtgccgtaa aagagattgg gcgcaagggc gatgacggtt tccacgtagt
     2461 tgccctccac cagatattgg cggattttct gctctgcgcc gccgcgatag aaaatgccgg
     2521 ggaatgagac gatggcggcg cggcctctgc cggaaaggta gttcagtgcg tgcagaatga
     2581 aggcaaaatc ggctttggat ttcggggcga gtacgcctgc aggggcaaag cggtcgtcgt
     2641 tgattaaggt ggggtcgccg ctgcctatcc agttgatgga atagggcgga ttggaaacga
     2701 cggcatcaaa gggtttgctg tctttgagtt tgggattggt cagtgtgtcg cccaattcga
     2761 tgtggaattt gttgtaattg acgttgtgca gaaacatatt catgcgggcg aggttgtagg
     2821 tggtgtggtt gatttcctgc ccgaagaagc cttcttcgat gatgtgctcg tcaaactgtt
     2881 ttttcgcctg caagagcagg ctgcccgagc cgcaagctgg gtcgtagatt ttgtttactt
     2941 tctcctgccc gtgcaccgcc agccgcgcaa tcagcttgga tacgctttgc ggggtgaaaa
     3001 attcgccgcc ggatttgcct gcgttggcag cgtagttgga aatcaggtat tcgtaggcat
     3061 cgccgaaaag gtcgatgtgg tggtcttcaa aactgccgaa atcgagttcc gccacgcctt
     3121 ttaggacggc ggcaaggcgt ttgttcttgt cggcaacggt gctgccgagc cggctgctgg
     3181 tggtgtcgaa gtcgtcaaac aggcctttaa tgtcttgttc ggacggatag ccggaggcgg
     3241 agctttcaat cgcggtaaaa atttctttca gcttggtgtt gagctcttcg ttttgatggg
     3301 cttcggcggc aatattgcaa aaaagctggc cggggtagat gaaatagcct ttgactttga
     3361 cggcatcgtc tttgatttcg ggcgtgatga tgctgtccgg catagcggcg taatcaatac
     3421 tgctgtcgcc tgcctgcata tagtcggtaa agttttcgct gataaagcgg tagaaaagtg
     3481 tgccgagaac gtattgttta aagtcccagc cgtccaccgc gccgcgtact tcgtcggcaa
     3541 ttttccaaat ttggcggtgc agttgggcgc gttgttgcat ttcggtcatc atcgaaatcc
     3601 atataaaagt taaacaaatc gaaatcgcct gatattttca gacgattttt tacgggcatt
     3661 caaattaagc caaattttcc agcatccatt tgacgtagcg ctcgacgcct gatttgacat
     3721 cgaaaaattc ttccgtataa cctgcctcac gcaatttggt gatgtcggct tgagtgaagc
     3781 tttggtattt gcctttgagc gcatcgggaa atgggatgta gcggataagt tcttcttcta
     3841 ccagctcttt caagctcatt tcgggtttgc cttcggcggc gcggcaggcg ttgacggtgg
     3901 cggcggcgag ttcgttgaac tgttggctgc ggccggtgcc gaggttgtag atgccggaaa
     3961 gttcgggatg gtcgaagaag tagaggttga ctttggcaac gtcttcgacg ctgacaaagt
     4021 cgcgggtttg ttcgccgttg ccgtagccgt cgttgctgcc gaacaggttg acgtaaccgt
     4081 gttcgcggta ttggtggaaa tggtggaagg cgacggatgc catacggcct ttgtgttgtt
     4141 cgtgttgtcc gtacacgttg aagtagcgga agccgacgac ttgggcggtc aagccttctt
     4201 tcatgcggcg gcgcaatact tggtcaaaca ggaatttgga atagccgtac acattgagcg
     4261 gtttttcgag ttcgcgctct tcacggaaga tttcgccttt accatacacg gcggcactgg
     4321 aggcataaag gaagggaatg cgttcgtcct gacaccagtc caacaaatcc agcgtgtact
     4381 ggtagttgtt ttccatcata tacaaaccgt cgtgattcat ggtgtcggaa cacgcgcctt
     4441 gatggaaaac ggcttcgatg ttttgataag gtaaaatgtg ttccctcact tggcggatga
     4501 attcatgttt gtcgagataa tgggcgattt cgcactcggc aaggtttttg aatttttcgc
     4561 ctttgctcaa attatcgacg gcaacaatgt cagtaatacc gcgttgatta agtgctttga
     4621 cgatgttgct gccgataaag ccggccgcgc ctgttacgat gatggtcata ttcggtttcc
     4681 tttgttttga gtaaaattaa taaacttagt tacaagaaat catcgatatt gtcctgaaac
     4741 acaaatttgc gatgatattc caaatatttt tctcttcctt cgggatatat tctcatctta
     4801 attttttgca tttggattga tattaagttt ttcccatgtg taacggctta gtcgagtacc
     4861 gcaaatcaaa cgaccatcat ccaagaaagt aatataacct tgatcgaaca accaatcata
     4921 agtcggcgtt aaagctaacc catttaaata atctaatgcc tctttctcat ttttctcagt
     4981 aatacaaacc atataaggtt taatatgact agcccttaac aaaatctcat ctgtaattaa
     5041 tgtaaatgga cattgcggca tatgatttat aacatccttg cgatatttct gctgcccttt
     5101 tctacttttc tgtttaatga tttcttgctc tacgaattga ttaaattctg aagccggtat
     5161 ttctaatttt tctcttgata agagttgcgg gtgcacgata gagcgaaatt gataatccaa
     5221 aaatattcgg aaataaaata atggttctga atcttctata tctttgacag gcaatagttt
     5281 aagaattgat aaataactaa ttttgggcaa tacaatctta cgccaaattg accatatatc
     5341 atcctccgaa cggatataac ccctacggta acctttagaa ttttccaaat ggtctgatac
     5401 atcatggatt gaaaagaaaa tattttctgt aggcagatta ttaatactta aaacgtaatc
     5461 atcataaaac cttgaaatat catgatgata tttctgttct tgtttgtgat attcaacttt
     5521 tgcatcaacc aaataacgct gtaaattttt cttattaaaa aaacaatttt tctgaatgaa
     5581 aggatattct accctccttc cattttttgt ctcagtaatc ccccaatttt catactcaaa
     5641 aaatgtactt agttttcgac cactatcatc aatataacta cctacatatt tgcgtgcttc
     5701 cccattacca ccaaaaacag ataacttgtt attcctgtga ataaaactat cttccgcacg
     5761 taaattttga atactatcga caataaaata ctctatgttt tcaatagtaa ttctttgaaa
     5821 atccggcatt ttactctcca taaagcgtaa tatccatttg ttttaatata gcgattaata
     5881 catcaacaac aatactgttt cccgcttggc gatacatttg agtatcacta actaaaattt
     5941 taaaatcatc tctaaaaccc attagccgca aacattccct aggtgttaat ttacgaatac
     6001 gtccacgatt atgagtaaca taattatcaa ccccggcacg atgcatttta tgcatagttt
     6061 gcaataatgg gcgagctaca ggcaaatcag tttccgtact ggttttaaaa ttttttgttc
     6121 ctcctgccaa aacataattt ttgatttttt cagaaagata atatttttcc tcaacgttat
     6181 ttacatcaaa aataaagtca tcaaattcag attcgcgggc tgcctgaaaa ataaaatcac
     6241 catgccaatt aaattgttga tttgcttttt ggcataaggc aatttctcca ttaatctgtg
     6301 tataacgttt ttgtctattt ttagaactgg taacaaattt cacacccttt tcacgtaaaa
     6361 aatatttgct atcagtatag tcctccaaaa aatcttgcat agtatgttct aattcaatct
     6421 tttcaggaaa ctgaaaacca tttataggag gggtatgaaa gccaacaaca aaaatacgct
     6481 cacgatgttg aggaatccca taatccttac tattcattat ttggaaatat aagtcataac
     6541 caagtgaata aaaaacactt tttacaactt tccaagtttt tccattatca tgattgagca
     6601 agccctttac attttcataa ataaatatct ttggttggac ttcatccaca acgcgagcaa
     6661 attcatagaa taatgttcct cgtgtatctt ctaatcctgc acgtttgcca accatggaaa
     6721 atgcttggca aggactgcct ccaactaaaa tatcaacttg atttctaaac tttctcgcat
     6781 caaattgagt aatgtcgtta tgccaaaaat cttcatttaa tttatagttt ccaagataac
     6841 tttttttaac gtatggatca atatctcctg caaaaacaat ggtatggttt aaatttaatc
     6901 ggtggaatgc ctgttcaacc gcaccaatcc cgctgaatac ggttgctaat ctaatatgtg
     6961 aatcaggttt aagaaaagtt ttagatttcc aaccttgttg actgggaaag agcaaagttt
     7021 tttgtaatcg agtatcgtgt gtctgtgcca ttgtcgaaat agtcatactt atatcgttct
     7081 gtttatctta tcaatatgaa aactacattg ttgattgccc tgacaatgcc ttgatcaatt
     7141 cggcaaacga gcaaaccgcc gtaccgagtt tcgccacgac aaccccggcc gcagtattgg
     7201 caaggtacat ggcttcgggc atggtgcagc ctgccgccaa acccaagccc attccggcaa
     7261 tgacggtgtc gcccgcaccg gatacgtcgt aaacttcttg ggcgcgggtg ggctggtaaa
     7321 tcggttcgcc ttcgctgaac aaggtcatgc cttcttcgct tcgggtcagt aaaacggcgg
     7381 tcaggtcgag gtggcggcgc aggttttgcg ctttttcggt cagctcgctt tcgtttttcc
     7441 aactgccgac cacttctttc aattcggcgc ggttaggcgt aatcagagtt gcaccgacat
     7501 atttttcgta atcgtcgcct ttggggtcga ttaagacggt tttgccggcg tgtttcgccc
     7561 aatcgatcat atcggagata tgcgacaggc cgcctttgcc gtagtctgaa aaaatgattg
     7621 cgtcgtattc gggcaagatt tcgcggtatt tctgcttgat ttgttccaac acttcgcagt
     7681 tgggatgttc ttcaaaatca agacggataa gctgctggtt gcgggcgacg acgcgcagtt
     7741 tgacggtggt ggcgatttgt ttgtcgcgca tcagatagga ggcgacgccg tcctgcacca
     7801 tcagcgcatc gagcgcgtcg gcggcttcgt cgttgccggt tacggacaac agccctgccc
     7861 tgccgcccaa cgaagcgatg ttgcgcgcga catttgccgc tccgcccgcg cgttggtcga
     7921 ttcgtccgat tttcgccacc ggcacggggg cttcgggcga aatacgggac acatcgccga
     7981 accaatagcg gtcgagcatc acgtcgccga caaccaggac tttggcttgc gcgaaacggg
     8041 atttgagggt ttcttgttgg aacttggcgg acatattgcc tccgttgtat tttttcagac
     8101 ggcctcagcc tgtaaggccg tctgaaaatc agtttgcttc aaggtttgcc acgcggttca
     8161 cttcgcgcaa tacggctacc ggatcggcag cttgggttac aggacgaccc attaccaaat
     8221 aagtcgaagc ggcagccaag gcttcggccg gtgtcatgat gcggcgctgg tcatcattat
     8281 tgccggcaac gtccaagcgg atgccgggcg tgaccaagac aaaatcttgt cccaactcgc
     8341 ggcgcagcgg tgcggcttct tgggcggaac agaccacgcc gtccaagccc gaactttgcg
     8401 ccagttttgc caagcggatg acttgttctt caggggcggt gttcaaaccg atttccgcca
     8461 aatcgctttg ttccatgctg gtcaacacgg ttacgccgat taagagcggc tttgtaccgt
     8521 atccggcaac ggcttctgct gcggcttcca tcatacggcg gccgcccgat gcgtgcatat
     8581 cgaccatcca aacgcccata tcggcagcga ctttgcaggc ttgcgcgacg gtgtggggaa
     8641 tatcgtggta tttcaaatcg aggaaaagtt tgaaaccttg attgattaag ctttctgcca
     8701 aattgcgtcc cgtcgcggta aacagctctt tgccgatttt gatttgacac aatgtcgggt
     8761 caaggttgcg gacgaatccg agcgtgtctt tttcgttgga aaaatcaagg gcgacgatga
     8821 cgggggtgcg ttgttgcgga gtttggaagt cggagattaa aggattcatg gtgttttcgt
     8881 ttcgggtttt cagacgacct ttatcgtctt tttcgcagta tagtggatta actttaaacc
     8941 ggtacggcgt tgtctcgcct tagttcaaag agaacgcttc tctaaggtgc tgaagcacaa
     9001 agtgaatcgg ttccgtactg tttgtactgc ctgcggcttc gtcgccttgt cctgattttt
     9061 gttaatccac
//
LOCUS       DAWHXS010000077         9032 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWHXS010000077 DAWHXS010000000
VERSION     DAWHXS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.077"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            33..1925
                     /gene="mafB"
                     /locus_tag="WIE22_001495"
     CDS             33..1925
                     /gene="mafB"
                     /locus_tag="WIE22_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ4127563.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISESSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGEIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEMKKGRGG
                     KPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSKSGVEIEGFTEPNRTAY
                     PLYNSK"
     gene            1952..2371
                     /locus_tag="WIE22_001496"
     CDS             1952..2371
                     /locus_tag="WIE22_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127564.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            2449..2532
                     /locus_tag="WIE22_001497"
                     /pseudo
     CDS             2449..2532
                     /locus_tag="WIE22_001497"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2529..2771
                     /locus_tag="WIE22_001498"
     CDS             2529..2771
                     /locus_tag="WIE22_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ4127565.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            2849..3094
                     /locus_tag="WIE22_001499"
     CDS             2849..3094
                     /locus_tag="WIE22_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4127566.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PNYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            3097..3486
                     /locus_tag="WIE22_001500"
     CDS             3097..3486
                     /locus_tag="WIE22_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127567.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSCSLSKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            3513..3704
                     /locus_tag="WIE22_001501"
     CDS             3513..3704
                     /locus_tag="WIE22_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ4127568.1"
                     /translation="MRLSDGIFDTKQVTKRVFDGKGEAKIPSYTWHHHQDTGRMQLIS
                     ERKHTQTGHIGGEAMDKGK"
     gene            3707..4105
                     /locus_tag="WIE22_001502"
     CDS             3707..4105
                     /locus_tag="WIE22_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127569.1"
                     /translation="MLGLTRNNCDDLGFAILCYERNAADKKEFLDWLYGIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQEFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            4206..4595
                     /locus_tag="WIE22_001503"
     CDS             4206..4595
                     /locus_tag="WIE22_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127570.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            4743..5132
                     /locus_tag="WIE22_001504"
     CDS             4743..5132
                     /locus_tag="WIE22_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127571.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(5173..6630)
                     /locus_tag="WIE22_001505"
     CDS             complement(5173..6630)
                     /locus_tag="WIE22_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ4127572.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(6645..7475)
                     /locus_tag="WIE22_001506"
     CDS             complement(6645..7475)
                     /locus_tag="WIE22_001506"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ4127573.1"
                     /translation="MAHYAIGDIQGCFDELTSLLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(7553..7942)
                     /locus_tag="WIE22_001507"
     CDS             complement(7553..7942)
                     /locus_tag="WIE22_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ4127574.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(8078..8602)
                     /gene="nspA"
                     /locus_tag="WIE22_001508"
     CDS             complement(8078..8602)
                     /gene="nspA"
                     /locus_tag="WIE22_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ4127575.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGMRVKF"
     gene            complement(8773..8967)
                     /locus_tag="WIE22_001509"
     CDS             complement(8773..8967)
                     /locus_tag="WIE22_001509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127576.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
BASE COUNT         2450 a         1833 c         2486 g         2263 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 attcttttat ccggcaggtt ctcgaccgtc agcattttga acccgacggg aaataccacc
      181 tattcggcag caggggggaa cttgccgagc gcagcggtca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgatgaa gccggtagtc ccgttgacgg atttagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgctg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcaggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aagctcaaac attgctgtta tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tacggcagtc atccccatca aagggattgg agctgttcgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctgtca agcggtcgca gatgggcgag atcgcattgc
     1081 cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gaaagaatgt gaaactggca aacaaacgcc
     1261 acccgaagac caaagtgccg tttgacggta aagggtttcc gaattttgag aagcacgtga
     1321 aatatgatac gaagctcgat attcaagaat tatcgggggg cggtatacct aaggctaagc
     1381 ctgtgtttga tgcgaaaccg agatgggagg ttgataggaa gcttaataaa ttgacaactc
     1441 gtgagcaggt ggagaaaaat gttcaggaaa cgagaagaag gagtcagagt agtcagttta
     1501 aagcccatgc gcaacgagaa tgggaaaata aaacagggtt agattttaat cattttatag
     1561 gtggtgatat caataagaaa ggcacagtaa caggagggca tagtctaacc cgtggtgatg
     1621 tacgggtgat acaacaaacc tcggcacctg ataaacatgg ggtttatcaa gcgacagtgg
     1681 aaattaaaaa gcctgatgga agttgggaaa tgaaaaaagg aaggggcgga aaacctatga
     1741 caaatcattc tatgttccca aaagattggg atgaggctag aattagggct gaagttactt
     1801 cggcttggga gaaaagaaag gaattagaag gtcaaaaatg gagaggtaca agtaaatctg
     1861 gtgttgaaat agaaggattt acggaaccta atcgaacagc ttatccactt tataatagta
     1921 aatgaagtat tgattaaaaa ggtgttttta aatgtataaa ataaaatgtt tttatgatca
     1981 gtttagttct ggtgaattat ttaactactg ccagttttta gataattctt ctaatcaaaa
     2041 gataaataca cgaggaacgg tgtatcaata tattatttat gttttaacag gagaccttta
     2101 tctacaaaaa gacatagatg aaaatttaga atttatccat caagcagaaa ataatcccaa
     2161 tgaagtttat tcaggaggtg gacaaggttt ttgctgggat atttctgctg aaaaagtagt
     2221 tttctatcac aacgaatttg atgaagaaga cggctggcct gatttaagct gttcgctgca
     2281 tacttttaaa actgctttaa ttgcttggaa tgcttttttg caattgccta aaagtattca
     2341 ttcggtggtg gagactgtga ttgaggaata agcataatta gcttaatgaa tagaatcagc
     2401 gatatagatt ggactgcaaa tccacgctta tacgctgtgc catgattaac aggaagagag
     2461 caacttggat gggctagtag ggatggtaag catttaaata tatcaattga tggaaagatt
     2521 acacacaaat gactaattta aaattagatt tctactctga agttattata aaagattctt
     2581 gccctaatga tttgttagaa aatggtgaga ctattaaagg aaaaaaagga gtggtattag
     2641 gtataagcga agaagatggt ataatctatg gctatactat tttacttttt gatataaaat
     2701 attgtatata tatagataag aaatatatta taccgactgg aaaaaaattc tctcgcgatg
     2761 atttttatta gctaatttaa gtaatttaag caagtttttt agatactgaa actggtaatt
     2821 atgtttatgt aagcataagg agttcagatt gaaggattct acagggcaaa tgaggctggc
     2881 aaccaaggat ttggcggaag ccattaaacg aggagaagta cgtagttctg cttttacaac
     2941 aaagcaacta aaggcaatcg aaaaaggaga atctaaaata ccaaattaca cttggcatca
     3001 tcatcaagac actggaagga tgcaattgat taatgaagac ttgcatcatg ataccggcca
     3061 tatcggttgg agagcgatga gtaaaggaaa gtaactatgt ggaaaatcat aaaagaggat
     3121 agtaatgatt taggatttgc aattaaatgc ttattctctc agtctattga tttaaatgaa
     3181 ttcaaattat ggattgaaca agtaatacga gatatgccca tcgaggacat cccgttttat
     3241 atttttgatt tggcggattt tgatggggga attgccgata ttgacaatat tgtaggtttt
     3301 gtttcgagtt gcagcctatc aaaatcgaaa aaaaatgcct tgacgggcat tgccttctta
     3361 aggggtatag atgtctatga ccctcccgtt tcaaaagaaa aagcattaaa agccttagag
     3421 aaatatcctg aaatttatca gaagtttcag catttctttc cgtttgtaga gctgcctccg
     3481 ctttaaacag tcaaaatgcc gtctgaaaca atattcggct ttcagacggc atttttgata
     3541 caaagcaggt aaccaaaaga gtgtttgacg gaaaaggaga agctaaaata ccgagctata
     3601 cttggcatca tcatcaggat acgggacgaa tgcagttaat atccgaaaga aagcatacgc
     3661 aaacagggca tataggtggt gaagcaatgg ataaaggaaa atgattatgt tggggcttac
     3721 acggaacaat tgtgatgatt tgggatttgc tatattgtgt tatgagcgca atgcagccga
     3781 caaaaaagaa ttcttggatt ggttatatgg aattgtcagg actgtccctg ccgaaagttt
     3841 tccaaggtat ctgattgatt taatagattg tttggaatca gatgattgtt ccataaatat
     3901 atataatatt attggctttg ttccaactac cggtatgacg aaaaaggaaa aagatgccgt
     3961 atatggcatt acctatatac ggaatcaaga gttttacgat attccgatta ctagggagaa
     4021 ggcgttaaaa gccttaaaaa aacatcctga aatttatcag aagtttcggc atttctttcc
     4081 gtttgtagaa ctgcccccgc tttaaacagt caaaatgccg tctgaaacga tattcggctt
     4141 tcagacggta tttttgatat aaagcgggta actaaaagag cgtttgacgg caaaggaaga
     4201 taattatgtg gaaaattata aaagaggata gtaatgattt aggattcgca attaaatgct
     4261 tattctctca atctattgat ttaaatgaat tcaagttatg gattgaacaa gtaatacgag
     4321 atatgcccat tgaggacatc cctttttata tttttgattt ggcggatttc gatgtaggaa
     4381 ttggcgatat tggcaatatt gtaggttttg ctccgagttc cagcctgccg aaatcgaaaa
     4441 aaaatgcctt gaccggcatt gccttcttaa gggggataga tgtctatgac cctcccgttt
     4501 caaaagaaaa agcattaaaa gccttaaaga aacatcctga aatttatcag aaatttcagc
     4561 atttctttcc gtttgtagag ctgcctccgc tttaaacagt caaaatgccg tctgaaagac
     4621 cgtccgccgt tcagacggca tttttgatat aaagcggata actaaaagag cgtttgacgg
     4681 caaaggagaa tctaaaatac cggatgtatc ggttgggaag caatggataa aggtaaataa
     4741 ttatgtggaa aattagtaaa gaaaattgtg aagatttagg atttgcaata gtctgtatgt
     4801 tctatgatgc tattaatctt tctgaattta aattatggtt ggatatagtt gtcagagata
     4861 ctcctattga tacaattcca ttgtatattt ttgatttgat tgattttgat aagagtatag
     4921 gggaaattta tgatgtaatt ggagtcgtta attatggtta catttcaaat gatcaaaaaa
     4981 atgcattaac gggcattgcc ttcttaaggg ggatagatgt ctatgatccg cctatttcaa
     5041 aagaaaaagc attaaaagcc ttagagaaat atcctgaaat ttatcagagg tttcagcatt
     5101 tctttccttt tgtagagctt ccgctttttt aaaagacaat atgccgtctg aaaagttttc
     5161 agacggcatt ttttatttct tccagtaggc gggggtgaag aggatgaaga cggtgaagat
     5221 ttccagcctg cccaagagca tggcggtaac gcagatccat ttctgcatca cgtccaaacc
     5281 ggcgtaattg ccggcgggcc cgacttcgcc caggccgggg ccggcgttgg tgatgcaggc
     5341 gatgacggcg gtgaaggcgg tggtaaattc cataccgctc gccatcagca ggaagctgaa
     5401 gaggacgacg gtcataaagt agatgaagat gaaggacata acggtcagcg cgaggcggtc
     5461 gggtatggcc ttgccgctga ttttgacggt gcggacggct ttggggtgca gcagcaccat
     5521 catttcgcgc aggctgaatt tgaacaggac gagggcgcgt atggttttga tgccgccgcc
     5581 ggtcgagccg gagttggcga ggatgttggc gaggaaaaac atccacaggg aaatcaggag
     5641 cggccattgt gcgaagtcgg tgttggccag cccgtttgcc agtccgatgg agacgaagtt
     5701 gaaggcggtg tagcgcaggg attcggtaaa accggcgtaa tagccggtgt gccacaggta
     5761 cagggcggcg gcaaggatgc tgccggagag cagcagcagc atcgtccggc attcttcgtc
     5821 tttccaatag gttttgaggc tgcggctgtt gagggcggcg aaatggttgg caaaattgat
     5881 gccgccgaca atggtgaaaa cgatgatgac cgcttcgatg aggggggagt tgtaataagc
     5941 tatgctggca tcgtgggtgg aaaacccgcc cagcgagagg gtcgccatcg cgtgacagac
     6001 ggcatcgaac cagcccatcc cggcaaaatg caggcaggct gccgcgagga tggtgatcag
     6061 ggtgtagccg aaccagagtt ttttcgccac ttgggaaatg cgcggcgaca ttttgctttc
     6121 tttgtcaatg ccggggattt cggctttgaa taattgcgtg ccgcctacgc cgagcatagg
     6181 caggatggcg acggcaagga cgatgatgcc catcccgccc agccagttga gcatatgccg
     6241 ccaaaagttg acggaggggg cgagcccgtc gacgtggggg atgacggtcg cgccggtggt
     6301 ggtcagtccc gacatcgatt caaaaaatgc gtcggtaaag cccatattcg ggaaatacag
     6361 gtacatcggc atcgcagcca tagcggcaaa cgccagccac aacatcagga cgagggtgaa
     6421 gccgtcgcgc gggcgcagtt cgcgcctgaa ccggagggtg gcgagccgga cgatgtacga
     6481 gccggaaagg gtaacggtcg cggtggtggc gaaggcggtg tacgcgccgt ccgaaaaggc
     6541 gtaggagagg gcggcgggta tcagcaggat aaaggaaaac agcataccca gtcgggagag
     6601 gacgtgggcg atgggcagga ttttgtgcat agtggggcgg tccgttattt tgcgaagctt
     6661 ttccagtcta tgccgccggc ggcttggact tgggtaattt cttccgtttc gaggttgacg
     6721 gcggtcagct gtccgcccca cagcgcgccg gtgtccagcg agatgacgtt gtcggcattc
     6781 gtgtagccca gcgaggacca gtgtccgaag atgatggtgc ggtcgaggtt ttgccggtcg
     6841 ggggctttga accacgggcg caggtaaggc ggcatttttt tcactgtgga tttgtagtcg
     6901 aaatccagtt cgtttttaaa ggtcagggcg cgcatccgcg tgaaggcgtt gacgatgaag
     6961 cgcaggcggg catagccttc caaaccttcg tcccacgcgg ccggtttgtt gccgtacatt
     7021 ttggagaaga atttgacgta ttttttgccg cgcagttcgg cttcggcttc tctagcgagc
     7081 gattcggctt tggcgatgcg ccattgcggc aggatgccgg cgtgtaccat cacgcggctg
     7141 ccctcgcgta tcaacagcgg ttgcgcacgc agccagtcga gcattttttt tccgtcgggg
     7201 tgtttgagta tgggttcgat tgtgtcgctg cgtttgggcg cgccttcgcc gcagccgaca
     7261 gcgagcaggt gcaggtcgtg gttgccgagg acgatttgca cgctgttttc gtgccggatg
     7321 cagaattgca gcgtttcgag ggatttcggg ccgcggttga cgatatcacc cgtcagccag
     7381 agggtgtccg tgccgtggtt gaagccgatt ttgccgagca gtgaggtcag ttcgtcgaaa
     7441 cagccttgta tgtcgccgat tgcgtaatgt gccattgcag atgttgtgaa gtgggaaagt
     7501 gttgcggttc ggacggcatg gttttgaaat atcatgcagt ccgaacgtgg aattatgcgt
     7561 tcaaaacgag gacggcttcg gcttcgacct gcacgccttt gggcagcgag gcaacgccga
     7621 cggcggcgcg ggcggggaag ggctcggcga taaattccgc catgacttcg ttgaagacgg
     7681 caaaattgcc caagtcggtc aggtaggcgt tgagtttgac gatgtcggcc agcgtgccgc
     7741 ctgccgcttc ggcgacggct tgcaggtttt ggaacacttg gcgcgcttcg gcgcggaaat
     7801 cgccgttgcc gacgacggtc atcgtggcgg gatcgagggg gatttgaccg ctcatgtaaa
     7861 cggtgtcgcc tgctcggacg gcttggctgt acgcgccgat ggcggcgggg gctttgtcgg
     7921 tgtggatgat ggttttggac atttcggatt cctcaaaaaa tagggcggca gaagccgcag
     7981 cattcgggat tatcgtacaa aaccgccggc ttgtgtagtt gcggtggcag aaaacaaaac
     8041 cgccgaaggc tcggcggttt gcagaataag gcgcatatca gaatttgacg cgcataccgg
     8101 cggacagttc gccggaacgg acgtttttga cagtgttgac tttgccgatg tagttgtagc
     8161 ggtagccggc atccaaatcg acattcgggg taacggcata gcttacgccc gccaatacgc
     8221 cgaggccggt ggaggtttgg ctgaagctgt cgctgccgcc caagtcgacg gaggcgcggt
     8281 tgaggctcaa gcgcgcgccg agatacggtt tgacgggcga ttgggtgtcg aagtcgtaaa
     8341 tggcggacgc gccgatgctg taaagtttga aatcggtgga tggggcttta tagtttttgt
     8401 agcgcgtgta atcgacggcg aagcggaggt cgttgatgcg gtagcctgcg gagatgcgcg
     8461 ggctgaagcc tttggcagaa cctaaagagc ttgaggcttt tgcgtgtgcg gcatcggctt
     8521 ggacgtaaaa gccggatgcg ccttccgcca gtgcggcggc cgggagagcg agggcaatca
     8581 gtgcggcaag tgcttttttc atattttggt tcctttatgg tcagttagaa aaattgttaa
     8641 gaatccgtta aagaatcctg ctgtattata cttaaatttt ctttttgcat cgtaatattt
     8701 tcaatacttc aagatacgta gcggtatccg gctgctttgc cgacggcaaa gccgttaacc
     8761 cgcgcgttgc ctttaaatgg tggcggcggc atcacgcggc ggatgggtga aacttgcaaa
     8821 cggtttggaa aaaacagcgg tatctgtcgg attgttgcag gtgcaggcat acggttttgt
     8881 gtgcgtctgt gccttaagcg tcggacattt ccggcggcgg ctgtgccgtc tgaaacgccc
     8941 ggcgggggat gcggctgcgt tttccatcga taagcatatt ttccggacgc gttcggggcg
     9001 ggttttcccg ggcggccgcc gatttgtttg cg
//
LOCUS       DAWHXS010000078         8970 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWHXS010000078 DAWHXS010000000
VERSION     DAWHXS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.078"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(391..825)
                     /locus_tag="WIE22_001510"
     CDS             complement(391..825)
                     /locus_tag="WIE22_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ4127577.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIE22_001511"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WIE22_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ4127578.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WIE22_001512"
     CDS             complement(1556..2398)
                     /locus_tag="WIE22_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ4127579.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WIE22_001513"
     CDS             2653..3291
                     /locus_tag="WIE22_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ4127580.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WIE22_001514"
     CDS             complement(3743..4543)
                     /locus_tag="WIE22_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ4127581.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTMAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4679..5182)
                     /locus_tag="WIE22_001515"
     CDS             complement(4679..5182)
                     /locus_tag="WIE22_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127582.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5519..6160)
                     /locus_tag="WIE22_001516"
     CDS             complement(5519..6160)
                     /locus_tag="WIE22_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ4127583.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            6488..8701
                     /locus_tag="WIE22_001517"
     CDS             6488..8701
                     /locus_tag="WIE22_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ4127584.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LTFDGLFDIRAVRILVDTIPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT         2419 a         2380 c         1934 g         2237 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggtgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aatactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgagaaaa accactgctt ttcacgattt
     1681 taacggactc gacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccggtt cccttatctt
     1921 ccccattacc ctgctcgctg tctgctttgg cagaagcatt gctgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcaggct caggttgcgg ctcaggcgca gcaggcgcac
     2221 ctgcgccttc gggtgcaccg tcccctccgc caaaatcacc caaatcgaca aattcgataa
     2281 catttcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttca cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctactg ctgatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 agtgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc catcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gcttactgga taacaaagtc
     4741 caaaccaata gcaggcaaaa taaggcatcc accccccttc ttcattaagg atatatattg
     4801 agaaacaaat cgcaactaaa cagaaaaaac ttgggagata aagccatttc attcccctat
     4861 tcaagaatct agccaagata ggtattttgt attctacaaa aaagaaaggc atttccaagg
     4921 gaaacatgtc agataaaaac ttttgtttat tttttactat agatagaacc ttgcttctca
     4981 agagaaagcc attaataata ccgatgacag ctattaatat atagagaata gtataagtat
     5041 gaataatctt cattagacaa aaagaagaaa tggcagataa attacatacg atatattgga
     5101 atataaaata tttacggtct aaaccttgtt cagttgcaat ttttttaaaa ttgccttgca
     5161 taaaaaaatc aaaggcgtcc attaaactat ctttcacatt agaaatttaa aagctaaata
     5221 atacgacaaa caatgtgaag tactattcat ggtttattta aaaaataata ctattctgaa
     5281 cattatttag atacagaaat taacaaatta gaactaaaca agcttttaaa tactttaatt
     5341 ttattggaaa gctataaaag gaactataac tttacacact agtcacttct ttttaagagg
     5401 caaaagggat tgggaaggtc gtcttggaga taagcactgg tatttcggcc aatggtaaat
     5461 agagtttacc tcaaataggg tagaacctcc ttcatctgtc agttaataac agccactttt
     5521 acaatgccct gtcaaaataa agcggcacgc ccgatttttc actcatcgtc atcaaataac
     5581 ccatcacctt ttggggccat tcgatgccgc gcaccacggt cagattcctc aaaacgggga
     5641 aaaccaaaat atcctccata ccgattccgc cgttgatgcc gtctgaagca ccgtccatca
     5701 aattttccaa ctcttgcaaa tctgcgttta tgcgttcgag atattgggcg gttttattca
     5761 aattggcgga aaagctgccg atgcttttct cttttttgtc tgtaaaatat tttaccgctt
     5821 ccggcgttgc aaattcgggc agcccgattt tgatcacgcg cggctgcacc agtttgtcgt
     5881 tgtatccgcc caccttgtcc agccacgccc gtatctcggg gcggacttcg tctttcagac
     5941 ggtcttcgcg gtcgaaatgc cgcacaatgt ccaaactctc gcccataaac gaaccgtctt
     6001 ctttttgcag gacgggcact tgtttcgcac cgatcatacc gatcggcgtt gcctcgtcgt
     6061 cgtttgccag cacggcttct tcaacgtccg cgccaaacag cccggcagcc atccgcgcac
     6121 gcacacaaaa cgggcaatgg tcgtaaatat acagtttcat caaaatattc ctcgtcaacc
     6181 tgtcggtacc ggctacctta acaccccgcg ccacccgaaa caagtttatc ttcccgccta
     6241 tgcaccgtaa ataaataagc tgagaccttt gcaaaaatag tctgttaacg aaatttgacg
     6301 cataaaaatg cgccaaaaaa ttttcaattg cctaaaacct tcctaatatt gagcaaaaag
     6361 taggaaaaat cagaaaagtt ttgcattttg aaaatgagat tgagcataaa attttagtaa
     6421 cctatgttat tgcaaaggtc tcaagctgtt acaataaact cgtttttatc ggaacgaaag
     6481 acccatcatg accgccatca gcccgattca agacacgcaa agcgcgactc tgcaagaatt
     6541 gcgcgaatgg ttcgacagct actgcgccgc gctgccggac aacgataaaa aacttgtctt
     6601 agccgcccgt tcgctggcgg aagcacatta ccccgccgat gccgtcacgc cgtatggcga
     6661 gccgttgtcc gaccacttcc tcggcgcggc acaaatggtc gacgaactcg acctgctgcc
     6721 cgatgccgtc gccgccaccc tgcttgccga catcggacgc tacgtccccg attggaatct
     6781 attggtttcc gaacgctgca acagcaccgt cgccgagctg gtcaaaggtg tggacgaagt
     6841 gcagaaactc acccacttcg cccgggtgga cagcctcgcc acgccggaag aacgcgccca
     6901 gcaagcggaa accatgcgga aaatgctgct ggcgatggtt accgacatcc gcgtcgtgtt
     6961 aatcaaactg gcgatgcgta cgcgcaccct gcaattttta agcaacgccc ccgacagccc
     7021 cgaaaaacgc gccgttgcca aagaaaccct cgacatcttc gccccgctcg ccaaccgttt
     7081 gggcgtgtgg cagctcaaat ggcagctcga agatttgggc ttccgccatc aagaacccga
     7141 aaaataccgc gaaattgccc tgcttttgga cgaaaaacgc accgaacgcc tcgaatacat
     7201 cgaaaacttc ctcaatatcc tgcgtacgga actgaaaaaa tacaatattc attttgaagt
     7261 tgccggaaga ccgaagcaca tctactccat ttacaaaaaa atggtgaaga aaaagctcac
     7321 tttcgacggc ttgttcgaca tccgcgccgt acggattttg gtggacacca ttcccgagtg
     7381 ttataccacg ctgggcattg tccacagcct ctggcagccg attccgggcg agttcgacga
     7441 ctacatcgcc aacccgaaag gcaacggcta taaaagtttg cacaccgtta tcgtcggccc
     7501 ggaagacaaa ggcgtggaag tgcaaatccg caccttcgat atgcaccaat tcaacgaatt
     7561 tggtgttgcc gcccactggc gttataaaga gggcggcaaa ggcgattccg cctacgaaca
     7621 gaaaatcgct tggttgcgcc aactcttgga ctggcgcgaa aacatggcgg aaagcggcaa
     7681 ggaagacctc gccgccgcct tcaaaaccga gcttttcaac gacacgattt atgtcctgac
     7741 cccgcacggc aaagtcctct ccctgcccac gggcgcgacc cccatcgact tcgcctacgc
     7801 cctgcacagc agcatcggcg accgttgccg cggtgcgaaa gtcgaagggc agattgtgcc
     7861 gctgtccacc ccgctcgaaa acggacagcg cgtcgaaatc attaccgcca aagaagggca
     7921 tccttccgtc aactggcttt acgaaggctg ggtcaaatcc aacaaggcaa tcggcaaaat
     7981 ccgcgcctac atccgccagc aaaacgccga caccgtgcgc gaagaaggcc gcgtccaact
     8041 cgacaaacag cttgccaaac tcacgcccaa acccaacctg caagagcttg ccgaaaatct
     8101 cggctacaaa aagccagaag acctctacac cgccgtcgga caaggcgaaa tttccaaccg
     8161 cgccatccaa aaagcctgcg gcacgctgaa cgaaccgccg cccgtacccg tcagcgaaac
     8221 caccatcgtc aaacagtcca aaatcaaaaa aggcggcaaa aacggcgtgc tcatcgacgg
     8281 cgaagacggt ctgatgacca cgcttgccaa atgctgcaaa cccgcgccgc ccgacgacat
     8341 tgtcggcttc gttacccgcg atcgcggcat ttcggtacac cgcaaaacct gcccctcttt
     8401 ccgacacctc gccgaacacg cgcccgaaaa agtactggac gcaagttggg cggcgttgca
     8461 ggaaggacaa gtgttcgccg tcgatatcga aatccgcgcc caagaccgct ccgggctttt
     8521 gcgcgacgta tccgacgcgc tcgcccgcca caaactcaac gttaccgccg tgcaaaccca
     8581 gtcccgcgac ttggaagcca gtatgcggtt cacgctcgaa gtcaaacagg tcaacgacct
     8641 cccgcgcgtc ctcgccagcc tcggcgacgt caaaggcgta ttgagcgtta cccggcttta
     8701 aatacaaaaa tgccgtctga aagccgtata acgcttcaga cggcattttg attgacgggg
     8761 tttgctattt tttgttgcat agtcaattaa aaacaaaata gtacaatact caactttgaa
     8821 ggtctaacca tggcatactc tgcggactta agaaacaaag ctttaaacta tagtggatta
     8881 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg caaggcgagg
     8941 caacaccgta ctggtttaaa tttaatccac
//
LOCUS       DAWHXS010000079         8930 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWHXS010000079 DAWHXS010000000
VERSION     DAWHXS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.079"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..2034)
                     /locus_tag="WIE22_001518"
     CDS             complement(<1..2034)
                     /locus_tag="WIE22_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ4127585.1"
                     /translation="MPAGRLPRRCPMMTKFTDCTRSNRIQPPTHRGYILKNNRQIKLI
                     AASVAVAASFQAHAGLGGLNIQSNLDEPFSGSITVTGEEAKALLGGGSVTVSEKGLTA
                     KVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQVREYTAILDPVGYSPKTKSALSDGK
                     THRKTAPTAESQENQNAKALRKTDKKDSANAAVKPAYNGKTHTVRKGETVKQIAAAIR
                     PKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRIKAEQPKPQTAKPKAETAS
                     MPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAVRPEPVPAANTAASETAAESAPQEA
                     AASAIDTPTDETGNAVSEPVEQVSAEEETESGLFDGLFGGSYTLLLAGGGAALIALLL
                     LLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAEVETPATPEPAPKNDVNDTLA
                     LDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQNGILSGALTQDEETQKRADAD
                     WNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTAENKPETVDTDFSDNLPSNNH
                     IGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQTPEELHDFLKVYETDAVAET
                     APETPDFNAAADDLSALLQPAEAPSVEENITETVAETPDFNATADDLSALLQPS"
     gene            2240..2770
                     /locus_tag="WIE22_001519"
     CDS             2240..2770
                     /locus_tag="WIE22_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ4127586.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            2773..3063
                     /locus_tag="WIE22_001520"
     CDS             2773..3063
                     /locus_tag="WIE22_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ4127587.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            3063..3341
                     /locus_tag="WIE22_001521"
     CDS             3063..3341
                     /locus_tag="WIE22_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ4127588.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            3390..4256
                     /locus_tag="WIE22_001522"
     CDS             3390..4256
                     /locus_tag="WIE22_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4127589.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            4327..5085
                     /locus_tag="WIE22_001523"
     CDS             4327..5085
                     /locus_tag="WIE22_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4127590.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            5159..5650
                     /locus_tag="WIE22_001524"
     CDS             5159..5650
                     /locus_tag="WIE22_001524"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ4127591.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            5681..6691
                     /gene="dusA"
                     /locus_tag="WIE22_001525"
     CDS             5681..6691
                     /gene="dusA"
                     /locus_tag="WIE22_001525"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ4127592.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYMARQITRNAWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTANPIGYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(6761..7648)
                     /gene="gluQRS"
                     /locus_tag="WIE22_001526"
     CDS             complement(6761..7648)
                     /gene="gluQRS"
                     /locus_tag="WIE22_001526"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ4127593.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAANHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQTGARRGADGFVYNGRCRNPQQRPAPQGKQPAWRIRVPDRVIGFSDGIVGGYAQNL
                     AGDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(7665..7955)
                     /locus_tag="WIE22_001527"
     CDS             complement(7665..7955)
                     /locus_tag="WIE22_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127594.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            complement(8108..>8930)
                     /gene="tal"
                     /locus_tag="WIE22_001528"
     CDS             complement(8108..>8930)
                     /gene="tal"
                     /locus_tag="WIE22_001528"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ4127595.1"
                     /translation="AVADVRAACGVCLAEHESTGGKTGFVSLEVSPELSKDAQGTVEE
                     ARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVNLTLLFSRAQTLKAYAAYARG
                     IAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLKGKIAIALAKAAYQDWEQYFT
                     APEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSLIGAHTVNTVPDATLKAFIDH
                     GTAKATLTEGADEAQAQLAEIAALGIDVETLAARLQEDGLKQFEEAFEKLLAPLV"
BASE COUNT         1911 a         2210 c         2668 g         2141 t
ORIGIN      
        1 agaaggttga agtaatgcgg acaaatcgtc tgcggtggcg ttgaagtcgg gtgtttcggc
       61 aacggtttcc gttatatttt cctcaacgga cggtgcttcg gcaggttgaa gcaatgcgga
      121 caaatcgtct gcggcggcgt tgaaatcggg cgtttcaggc gcagtttccg cgacggcatc
      181 ggtttcgtac actttcagga aatcgtgcaa ctcttccggt gtttggactt cggcaactgt
      241 tttttccaag atggtttcgg gcgaggaagc cttcaggaag cctgccagtc cggagggtga
      301 ggcaggtttt gcggaagctg tttcttctgt gccgatatgg ttgtttgagg gcaggttgtc
      361 ggagaaatcg gtatcgacgg tttccggttt gttttcggca gtttgggcga cagattccgg
      421 ttcgggcgtg tcgatgacga tttcgacagg gttgtacggg ttgaaggtct cgggctcgta
      481 cacgctgtct gtggattcga tggcgttcca atcggcatcc gcgcgttttt gggtttcttc
      541 atcctgcgta agtgcgccgg ataaaatgcc gttttgcgcg gctgccaggc tgtcgaaatc
      601 caagtcgatg cggttggaag gcgtatcggt ttcgacatcg aacgtttgtt ttgccgataa
      661 ctcttcttca gattccccat ctaaggcaag tgtgtcgttt acatcgtttt tcggagcggg
      721 ttcgggcgtt gccggagttt cgacttcggc aaaggtgatt tctatgccgt cgtctgccgc
      781 gtcgtcaagg tcaggctctt cctcagggac ggattcttcg gtacggcgcg cgcgtttgga
      841 ttgggcaagg cgcaaaagca gcagcagggc gattaatgcc gcgcctccgc cggcaagcag
      901 caaggtgtac gaaccgccga acagaccgtc aaacagtccg ctttcggttt cttcttcggc
      961 agaaacctgt tcgacaggtt cggaaacggc gttaccggtt tcgtcggtcg gcgtgtcgat
     1021 ggcagaagcg gcggcttctt ggggggcgga ttcggcagcg gtttccgatg cggcagtatt
     1081 tgcagcgggt acaggttcgg gtcgaacggc cggtttttcc gcttttgctt cgggcgcggc
     1141 aacttttgct tcaggttttt caaccggttt ctctaccgtt gcctgtttgg acggttcgga
     1201 cggcatggat gcggtttcgg ctttgggttt cgccgtttgc ggtttgggtt gttccgcttt
     1261 gatcctgttc agattcggaa tgtgaagcac gctgcccgca cgcagtctgc cgtgtgcgga
     1321 aacatttggg tttgccttca gcagcgcatc ggcaacctgt tcgagcgtca ggtgtttcgg
     1381 gcggatggcg gcggcaatct gtttgaccgt ttcgcctttg cggacggtat gggttttgcc
     1441 gttgtacgcc ggtttgacgg ctgcgttcgc gctgtctttt ttatcggttt tgcggagggc
     1501 tttggcgttt tgattttctt gggactctgc tgtcggagcg gttttgcggt gtgtcttgcc
     1561 gtctgaaagt gcagatttgg ttttgggcga gtagccgaca ggatcgagga tggcggtgta
     1621 ttcgcgtacc tgtgcgcctg cgccgatgcg gaataccagg acgggatcgc ggactgcctg
     1681 ttcggaagaa acggcaatga cggctttgtc gcccaacttg tggactttgg cggtcagacc
     1741 tttttcggaa acggtaacgc tgccgccgcc tagcagggct ttggcttctt cgccggttac
     1801 ggtaatgctg ccggaaaagg gttcgtcaag gttggactgg atattcagtc cgcccagtcc
     1861 agcatgtgcc tgaaaggatg cggcaactgc gacggaggcg gcaatcagtt tgatttgtct
     1921 gttgtttttc aagatgtatc ccctgtgggt tggcggctga atacggtttg accgcgtaca
     1981 gtctgtaaat ttcgtcatca tcgggcatcg gcggggcagt cggccggcgg gcatttaata
     2041 tgtgaatgta ccgaccgccg ccacatttta aacggcaatc attcgccgtt tttacaaatt
     2101 atgacatatc tccatctttt ttcaaaaaca tctgtgcata tttgcatcaa tcaaaacaaa
     2161 atttgttggt tttgcaggtg caaaaacagg gttctgcctg tatgattagc gtttatttga
     2221 tttgctttct catttggata tgaaattcgt cagcgacctt ttgtccgtca tcctgttttt
     2281 cgccacctat accgttacca aaaatatgat tgccgctacg gcggtcgcct tggttgccgg
     2341 tgtggttcag gcggcttttc tgtattggaa atataaaaag ctggatacga tgcagtgggt
     2401 cggactggtg ctgattgtga tattcggtgg cgcaaccatt gttttgggcg acagccgctt
     2461 cattatgtgg aagccgagcg ttttgttttg gctgggcgcg ctgttcctgt ggggcagcca
     2521 cctcgccggt aaaaacggct tgaaggcgag tatcggcagg gagattcagc ttccggatgc
     2581 cgtatgggcg aaattgacgt atatgtgggt cggtttcctg atttttatgg gtatcgccaa
     2641 ctggtttgtg tttacccggt tcgagtcgca atgggtcaac tataaaatgt tcggctcgac
     2701 tgcactgatg cttgttttct ttattattca gggtatttat ctgagtacct gtctgaaaaa
     2761 ggaggattga ctgtggaata ttttatgttg ctggcaacag acggggagaa tgtgtatgaa
     2821 gcgcgtatgg cggcacgtcc cgaacacctc aaacggctgg agacgctgaa gtcggaaggc
     2881 cggctgttga cggcaggccc gaatcctttg ccggaggact ccaaccgcgt ttcgggcagt
     2941 ttgattgtgg cgcagttcga gtctttggat gcggcagagg cgtgggcgga agacgatccc
     3001 tatgttcatg caggcgtgta cagcgaagtg ctgatcaagc cgtttaaagc ggtgttcaaa
     3061 taatgccggc cgtcgatttg atccgcgaac gcctgcagac gctcgatccg ctggtgttgg
     3121 aaatcggcga tgagagccat ctgcacaaag gacacgcggg caataccggc ggcggacatt
     3181 atgccgtttt ggtcgttagc ggccgttttg aaggcgtaag ccgcctgaac cgccagaaaa
     3241 cggtcaaatc gctgctcaaa gatttgtttt caggcggcat gattcacgcg ctcggcatcc
     3301 gggcggctac ccctgacgag tatttccata cggcggactg aatgaagtct gcccgaacat
     3361 ttcaatttaa aatttaaaga gagaagatta tgaaagcaaa aatcctgact tccgttgcac
     3421 tgcttgcctg ttccggcagc ctgtttgccc aaacgctggc aaccgtcaac ggtcagaaaa
     3481 tcgacagttc cgtcatcgat gcgcaggttg ccgcattccg tgcggaaaac agccgtgccg
     3541 aagacacgcc gcaactgcgc caatccctgc tggaaaacga agtggtcaac accgtggtcg
     3601 cacaggaagt gaaacgcctg aaactcgacc ggtcggcaga gtttaaaaat gcgcttgcca
     3661 aattgcgtgc cgaagcgaaa aagtcgggcg acgacaagaa accgtccttc aaaaccgttt
     3721 ggcaggcggt aaaatatggc ttgaacggcg aggcatacgc gctgcatatc gccaaaaccc
     3781 aaccggtttc cgagcaggaa gtaaaagccg catatgacaa tatcagcggt ttttacaaag
     3841 gtacgcagga agtccagttg ggcgaaatcc tgaccgacaa ggaagaaaat gcaaaaaaag
     3901 cggttgccga cttgaaggcg aaaaaaggtt tcgatgccgt cttgaaacaa tattccctca
     3961 acgaccgtac caaacagacc ggcgcgccgg tcggatatgt gccgctgaaa gatttggaac
     4021 agggtgttcc gccgctttat caggcaatta aggacttgaa aaaaggcgaa tttacggcaa
     4081 cgccgctgaa aaacggcgat ttctacggcg tttattatgt caacgacagc cgcgaggtga
     4141 aagtgccttc ttttgatgaa atgaaaggac agattgccgg caaccttcag gcggaacgga
     4201 ttgaccgtgc cgtcggtgca ctgttgggca aggcaaacat caaacctgca aaataattct
     4261 gaaaacggga tacggcggca agacgttcag acaggcgttt tgccgccgcg caggacaggg
     4321 aataccatga aacagaaaaa aaccgctgcc gcagttattg ctgcaatgtt ggcaggtttt
     4381 gcggcagcca aagcacccga aatcgacccg gctttggtgg atacgctggt ggcgcagatc
     4441 atgcagcagg cagaccggca tgcggagcag tcccaaaaac cggacgggca ggcaatccga
     4501 aacgatgccg tccgtcggct gcaaactttg gaagttttga aaaacagggc attgaaggaa
     4561 ggtttggata aggataagga tgtccaaaac cgctttaaaa tcgccgaagc gtctttttat
     4621 gccgaggagt acgtccgttt tctggaacgt tcggaaacgg tttccgaaag cgcactgcgt
     4681 caattttatg agcggcaaat ccgcatgatc aaattgcagc aggtcagctt cgcaaccgaa
     4741 gaggaggcgc gtcaggcgca gcagctcctg ctcaaagggc tgtcttttga agggctgatg
     4801 aagcgttatc cgaacgacga gcaggctttt gacggtttca ttatggcgca gcagcttccc
     4861 gagccgctgg cttcgcagtt tgcagcgatg aatcggggcg acgttacccg cgatccggtc
     4921 aaattgggcg aacgctatta tctgttcaaa ctcagcgagg tcgggaaaaa ccccgacgcg
     4981 cagcctttcg agttggtcag aaaccagttg gaacaaggtt tgagacagga aaaagcccgc
     5041 ttgaaaatcg atgccatttt ggaagaaaac ggtgtcaaac cgtaatggca tttccaatac
     5101 cgatgccgtc tgaagccttt tcagacggca ttgcacgttc aggtaaggag gacggcttat
     5161 gcgtgcggtc atacagaaaa cggtaggtgc aaaggtggat gtcgtgtccg aaaacggcac
     5221 ggaaacctgt ggcaaaatcg acggcggatt cgtcgtgctg ctcggcgtaa cgcatagcga
     5281 cacagaaaaa gatgcgcact atatcgccga caaaatcgcc catttgcgcg tgtttgaaga
     5341 cgaagcgggc aagttgaacc tgtctttgaa agatgtcggc ggcgcggtgc tgctggtgtc
     5401 gcagtttaca ctttatgccg atgcggcaag cgggcggcgg ccttcgtttt cccaagccgc
     5461 acctgcagaa caggcgcagc agctttacct gcgaacggcg gaactgttgc gcggacacgg
     5521 gattcatgtc gaaacagggc gtttccgcac gcatatgcag gtgtcgctct gcaacgacgg
     5581 gccggtaacc atactgctgg actctttcat gacgcggatt tccccaaaaa tgaaggttgt
     5641 tccggattga aattgaatct gcaatgatga aatatcgaca atgaacgaca atacacacac
     5701 ccttcccccg cgccgccttt ccgtcgcccc gatgctcgac tggacggaca ggcactaccg
     5761 ttatatggcg cgccagatta cccgaaatgc ttggctttac agcgaaatgg tcaatgccgg
     5821 tgcgattgtt tacggcgaca aagaccgctt tttgatgttc aacgaaggcg agcagcccgt
     5881 cgccctgcaa ctgggcggca gcgatccgtc cgatttggcg aaagccgcca aagccgccga
     5941 agcatacggc tacaacgagg tcaacctcaa ctgcggctgc cccagtccgc gcgtgcagaa
     6001 aggttcgttc ggcgcgtgtc tgatgaacga agtcgggctg gttgccgact gcctcaacgc
     6061 catgcaggac gcggtcggta ttcccgttac cgtcaaacac cgcattggtg tggacaggca
     6121 gaccgaatac caaaccgttg ccgatttcgt cggcacgttg cgcgacaaaa ccgcctgcaa
     6181 aaccttcatc gtccacgccc gcaacgcttg gctggacggt ctttccccca aagaaaaccg
     6241 cgacgttccc ccgttgaaat acgattacgt ttaccgcctc aagcaggagt ttcccgggct
     6301 ggaaatcatc atcaacggcg gcatcaccac caacgaagca atcgccggac acctgcaaca
     6361 cgttgacggc gtgatggtcg ggcgcgaggc gtaccacaac ccgatggtga tgcgcgaatg
     6421 ggacagattg ttttacggcg acactgcgaa cccgatcgga tacgccgatt tggtgcaacg
     6481 cctctacaca tacagccaag cccaaatcca agccggacgc ggcacaatcc tgcgccatat
     6541 cgtccgccac agcctcgggc tgatgcacgg cttgaaaggc gcgcggactt ggcggcgtat
     6601 gctttccgac gcaacgctct tgaaagacaa cgacggcagc ctgattctcg aagcgtggaa
     6661 agaggtcgaa cgggcaaata cgcgcgaata gggcggggct gtatgtgtga aatgccgtct
     6721 gaaggcttca gacggcattt gtgcgtttgt cgggcggtgt ttagggggtg gtaatggcgt
     6781 gtttcggcac tttgtccata tcccagtgtg ccaccgccca gtcgagcagt tcggcagggc
     6841 ggtcggcttc cggtgcttcg ggcaggttga ggtaacggaa cacttggcgg aggagttgtt
     6901 cgcggcggtt taaatccaat gcgggtgcga gcgtctgttt cgaccatttc tgcccttgtg
     6961 cgttggtcag cagcggcagg tgggcatatt gcggtgtcgg aacgtccaaa cactgctgca
     7021 aatagatttg gcgcggcgta gaaacgagca ggtcttgtcc gcggacgatg tgggtaacgc
     7081 cctgttcggc atcgtcggca acgacggcga gttggtatgc ccagtaaccg tctgcacgaa
     7141 gcaggacgaa atcgccgatg tcgccggcga ggttttgggc gtaaccgccg acgatgccgt
     7201 ctgaaaaacc gataacgcgg tcggggacgc ggatgcgcca cgccggctgt ttgccttgcg
     7261 gtgcagggcg ttgctgcgga ttgcggcagc gtccgttata gacgaacccg tctgcgcccc
     7321 gccttgcccc ggtctgccag tctttgcggc tgcaatggca gggatagacc agtccggcgg
     7381 tttgcagacg gcatagtgtt tcttcataca gggcgtaacg gcggctctga taggcgactt
     7441 ctccgtccca ctcgaatccg aatgcctcaa gcgtgtgcag gatatggttt gccgcccccg
     7501 gcatttcgcg cggcggatcg aggtcttcca tgcggaccag ccatttgccg ccgtttgagc
     7561 gtgcatcggc ataggaagcg gcggcggtca gcagcgagcc gatgtggagc agcccggtcg
     7621 ggctgggggc gaaacgtcct gtgtacatat ccggtacggc ccctttattt aagactatta
     7681 atcaaagcca ttatctcatc tttattcagt tccatcccgg gttcttcaag taaggttaaa
     7741 tcatataggg cattatattg ctcttcggta gctgaaccat ccataaggcc ggacgagaaa
     7801 aaatcgaagg ctctatctgc gattctctca ttacttgcat ttctactaac cagtttcatc
     7861 aattctgtat atattgtatg gggaggtacc ggatccttaa aaatcagatc tgaataaatt
     7921 tcattgggtg aaatgatttc gagtgctttt gccatgattc tatttccttt tgtgttggtg
     7981 ggtaatgtcg tgcattaact tcttgcccat taatattttt agggtgaatc cttgatatgc
     8041 cgcactgtgt ccggtcaaac gggcgatgcc gtctgaaagc ctttcagacg gcatcgggat
     8101 gaaaagttta aaccaacggt gcgagcagtt tttcaaaggc ttcttcaaat tgtttcaaac
     8161 cgtcttcctg caaacgcgcc gccaaggttt cgacatcgat gccgagcgcg gcaatttcgg
     8221 caagctgcgc ttgcgcttcg tccgcgcctt cggtcagcgt ggctttggct gtgccgtggt
     8281 cgataaaggc tttgagcgtg gcatcgggaa cggtgttgac ggtgtgcgcg ccgatcaggc
     8341 tgtcaacgta gagcgtgtcg ggataggctg ggtttttcac gccggtagat gcccataaaa
     8401 gctgcacgcg gtttgcgcct tgggcttcca gtgcggcaaa ttcgggggcg gtgaaatatt
     8461 gttcccaatc ttgataggcg gctttggcaa gggcgatggc gattttgcct ttgagccggt
     8521 cgggcagtgt tgtgtccagc gcactgtcca cacgcgagat gaagaagctg gcgacgacgt
     8581 ggatatgggc aacgctttgt ccggctgcca agcgtttggc aatgccgcgc gcgtaggcgg
     8641 cgtaggcttt gagggtttgg gcgcgtgaga acagcagggt caggttcacg ctgatgccgt
     8701 ctgaaacgag ggtttcgagc gcatcgatgc ctgcgtcggt ggcaggcact ttaatcatcg
     8761 cgtttttgca cccgatggcg gcgtagaggc ggcgcgcttc ttcaaccgtg ccttgcgcgt
     8821 ctttggacaa ttcgggcgaa acttcgaggc tgacgaagcc ggttttgccg ccggtggatt
     8881 cgtgttcggc aagacagacg ccgcaggcgg cacgcacatc ggcaaccgcc
//
LOCUS       DAWHXS010000080         8871 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWHXS010000080 DAWHXS010000000
VERSION     DAWHXS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.080"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(65..1207)
                     /locus_tag="WIE22_001529"
     CDS             complement(65..1207)
                     /locus_tag="WIE22_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ4127596.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERNIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1256..1678)
                     /locus_tag="WIE22_001530"
     CDS             complement(1256..1678)
                     /locus_tag="WIE22_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127597.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1671..2480)
                     /locus_tag="WIE22_001531"
     CDS             complement(1671..2480)
                     /locus_tag="WIE22_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ4127598.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2562..2939)
                     /locus_tag="WIE22_001532"
     CDS             complement(2562..2939)
                     /locus_tag="WIE22_001532"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ4127599.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAVRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3139..3906)
                     /locus_tag="WIE22_001533"
     CDS             complement(3139..3906)
                     /locus_tag="WIE22_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ4127600.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMILFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIAKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3916..4695)
                     /locus_tag="WIE22_001534"
     CDS             complement(3916..4695)
                     /locus_tag="WIE22_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ4127601.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQRIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPA
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLINVIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHARTDYTDKNRHYIRARITV"
     gene            complement(4742..5470)
                     /gene="pdxJ"
                     /locus_tag="WIE22_001535"
     CDS             complement(4742..5470)
                     /gene="pdxJ"
                     /locus_tag="WIE22_001535"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ4127602.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5494..6285)
                     /gene="recO"
                     /locus_tag="WIE22_001536"
     CDS             complement(5494..6285)
                     /gene="recO"
                     /locus_tag="WIE22_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ4127603.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6319..7446)
                     /gene="pheA"
                     /locus_tag="WIE22_001537"
     CDS             complement(6319..7446)
                     /gene="pheA"
                     /locus_tag="WIE22_001537"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ4127604.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7485..8717)
                     /locus_tag="WIE22_001538"
     CDS             complement(7485..8717)
                     /locus_tag="WIE22_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ4127605.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVALMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQHFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
BASE COUNT         2088 a         2386 c         2316 g         2081 t
ORIGIN      
        1 tatcggccgg atattaaaaa aagccgtctg aacatttttc agacggcatt tttgtacact
       61 ttcctcaacg gttcgatccc tgcaccatat caatcaggaa caggcggcaa tcgaggttgc
      121 cgttataaag cggaattttg cgttttggcg acaggcgcat gaatttgggc atttccctgt
      181 cgccggtaaa catgccgacc cgccagccgg catagtgttt tttcaaccac gtacccaact
      241 gcggataaag cgcctgcaaa gcgtggactt cctcaaggcg cacgccgtag ggcggattgg
      301 acaccataat gccgttttcg ccgttcggtc ggacggactg cgcgtcggca acgccgaagg
      361 aaacgatgtc gtccacttcg gcactgcgtg cgttgtccaa tgccgtctga acgatgcggc
      421 ggtcgctgtc gctgcctgca atcggggcgc ggacggggcg ggtttgcgct tcggcgcggc
      481 ggcgcaaatc cgcccacagc gttttatcga aattttgcag tttttcaaaa ccgaagcggc
      541 gcatcatgcc cggcgcgcgg cgggcggcaa tccaagcggc ttcgatagca atcgtgccgc
      601 tgccgcaaaa cgggtcttga aacggctgcg tgccgtcgta gcctgccgag agcagcaatc
      661 cggcggcaag gttttcgcgc agcggggctt cgccggtatc gagacggtag ccgcgtttga
      721 acagggcttc gcccgaagtg tcgatgaaga tttcgacatt gcgttcgtcc aaaaaggcgt
      781 ggatgcgaac atcgggcgcg gctttgtcca cgctcggacg tgcgtcgtaa atgtcgcgga
      841 aagcgtcgca gacggcatct ttgacggtca gtccgacaaa ttggatgctc ttaacgttgg
      901 cacgctttgc ctcgactttg actttgaacg tctgctgtaa agtaaaccaa ttaaaccagt
      961 tgatattttt ggcgagtttg tagatgttgc gctcattgcg gtatgtccct ttggtcaggc
     1021 gcagcaggat acggctggca gtacgcgaat gcaggttggc ggcgtaaacc tgttccaatc
     1081 cgccccggca ggaaacgccg ccgtcaaaca cttgtacatc ggtacagccg aggctttcga
     1141 gttcttgaga taaaacggtc tccaagccgc gcgggcaggt ggcgaaaagt gtatataaag
     1201 tattcataac gtgtcctttc cggacggatg ttccggcaat ggcggattga aaggtttatt
     1261 cggcggtaaa tgccgtctga aatgtccgac tgccgattgt aatgtctgcc gtaaaatcgc
     1321 gcctgccttc gacacagatg ggcaggcgga tgcgtactgc ctgccaagtc cccgacggtt
     1381 gccgctcgaa catatagcgg ttgaaaccca tatccatatt tttcatactg aagctgatgc
     1441 tgacctgttc cgtgccggcg ggcgcgtgtt cgatataaat atcaaacggt tttttggttg
     1501 aaacggcggc ggcgcggacg cggcttccgt ccggcagcgt gcaaccctcg gtcaaatcgc
     1561 attgcgccgc cacagcttgc ggctgctgcg cctgccacca ttgcaacaaa acgagcttga
     1621 cggcggcaaa ggcaatcagc agcagtgcgg caagcagtaa tttacggttt ttattcatga
     1681 ttcttcccta ttgtttcatc ccttaccacc acgtgcgccc ccaatccgag ccatagtttt
     1741 ccataacgtg caaccgtctg tccgtttgct gcaagtacga gcggcacgga tacgccctgc
     1801 cgcagggctt ctgccgcctc ttgtgcctga acatcattgc acacgcgcca cacgacaaca
     1861 tccgcgccct tttcctgcgc ctgccgccat tgttcgcggg tctgcaccat catccacacg
     1921 ctgtcggcgt gttcgggtct gtcctgccat tgcgccgcct ccatcaaaac gttcgcaccg
     1981 cttccctctg ccgaacccaa aggcaggacg cggtacgcgc ccatactgtt ctccccgtgc
     2041 aaacccgttt tcaggctgcc gtacaaacgg cgcggaacgg acagcgaacg caaaagcgcg
     2101 ccgttggcgg gcagcatggg ggcaacggta aaatcacccg ccttctgcca agaccattcc
     2161 tgcccttcgc gggattgcgg ttcgcccgtc cattggccgg ggttgaccca taggaatttc
     2221 aggcagacgc gggcgtgttc gtaggaatgg attttggtca gccaaggcgt ggcggcaagg
     2281 atgcggatgc cgagttcttc ttcaaactcg cgttgcaagg cttggaagtc ggtttcgccc
     2341 gcttcgacct tgccgccggc aaattcccaa tatccggcat agggtttgcc ttcggggcgc
     2401 gagctgagca ggtagttgcc gtctgaatcg agcaggatgc cggcaacgac gcggataagg
     2461 ggtcgggtgt cttgaatcat ggcgggtacg cggttggtcg gtcggagtgc aaatgaggga
     2521 cgagcaatgc cgtcaacggg tttgccgcat ttcagacggc attatttttc ggcaacgaca
     2581 aacgccaata cggtgtcttc ttcgtcgctc atgctgaggc tgacgcggct gatgccttgt
     2641 tcctccagcc atttggacag ggcggggccg tagaaaaatt cgggcttgcc caatgcgtca
     2701 tgcccgatgc cgatgttgcg gaaggaaacc gcgccgcgta tgcccgtgcc gacggctttg
     2761 gcaaaggctt ctttggcggc aaagcgtttg gcaaggtagt tgacgggttt gcccgcctga
     2821 ggaaattcaa gcagctcttc cggagtgagg atacgcacgg caaacgcctg tccgaatttt
     2881 ttgtttaagc ggatgatgcg cttgagggaa acgatgtctg taccgatgcc gtaaatcata
     2941 tttgcgctcc ttcgcccttg gttgcgggta gtgatgaatg tgatgatgaa tgccgtcaat
     3001 ttcttggggc gttaaaatgg cgtgaatatc cactttcggg gttttgtcgg taacaatttt
     3061 tattgtaacc ttttgcattt gaaattctaa aacatcgtgc caattattta ataaggagtt
     3121 aaggcattta ataatgaatt aaagtttgat taaacccgaa tgcccgattt tcagagccaa
     3181 taccaataaa acaataaaaa ttaacatttt aaaaaaattt gggctaatct tagtccttaa
     3241 ccgaattcca acatacaatc caataacaga caatacggac agtaaaaata ttaaaccgta
     3301 ttcactctta tttaataacc aatactggtc tcttagcata tatatttgaa caattttcgc
     3361 caaaagatag catagattgc ttgattttgc gatacgattt ttattctctg tttcgctaag
     3421 caaaaatatt aacaatatgg gagacatggc attggttgaa ccgccgatga tgcctgccaa
     3481 aaacccaaaa agaatcatat tcttattatt ggcaactact tgaatatttt ttgcttttgc
     3541 acatacattt aaaataccat taacagaata atacaatgta atgattgcca tcagtaaaag
     3601 cagccacgac actggaagta tcaaaagcaa cttcaccccc aaaatgctgc caacgacgct
     3661 gccgatagca agcaatttat aggtttttaa ataataaaca atctcttgcc aaaaaccctt
     3721 tttgttattg ctgcatagaa ccaacaagct cattaacagg cttggtaatg ccaccaaggc
     3781 aacaacctta gacaatggca tgataaaagc caatgcggtt gtaccgagca tcggaaatcc
     3841 catgcctgta attccgtgca gtattgcggc agcaacaaaa acgatagatt gcattatttc
     3901 ttgcataacc gtcctttata cagtaattct tgctctaata taatgcctgt ttttatccgt
     3961 ataatcagtt ctggcatgca agccattgac attgtcaata ataatcaaac catcatcttg
     4021 tgcagaaaat tcttgaatat cactcgcatt ttttataaca tttataagat aatcaagtgc
     4081 aagtaccatt tcctttgata ctttatttct attttcttca atacctttat agatacaatc
     4141 acttctaaat ctaatcattt gagtattgac cgataagata ttaccccatc tcacaccttg
     4201 ttttttatca aatgatgctg gtgttttaaa tggatataaa ttgcccgtta atgtttttaa
     4261 gtgtttttta ccggtttctg ttttagataa ctgattgaca atatctgatg aacttaaaaa
     4321 tagggaatta cctccatcat tggctgattt tactacatac ataaccaaat aactttccgg
     4381 gttttcacta aaagatgaat cactatgaag tggacattct ccaacatctt ccgagaaagt
     4441 tatatcatta tttattacat agtcatcgcc gcgatattta atatcccaaa atatagattt
     4501 aaaaccatcg tctttgtggt ttgtcagcat tcctaattga gatagaaagt caaataattt
     4561 cttgtttctc cggctgtcat caagatggtt tagattaaga ccgcgaacaa tacaataccc
     4621 aatacgctgt actgtattgt gaatttcttc tatcaaacta ttattgtttg cagataaaat
     4681 atacttttga ttcataacat actcctgtcg tgaaatgccg tctgaaacgg tttgcctgcc
     4741 cttacggcag cagccttgcc ctgaacatcg cctccttcat ttggcgcacg gcctcgggca
     4801 atccgaggaa aagtgcctgc gaaatcagcg aatgcccgat gttcagttcg cggatggcga
     4861 ggatttgggc gatgggggta acgttgtgta tggtcagtcc gtgtccggcg ttgacgacca
     4921 agcccaaatc gctggcaaaa tgcgcgccgt tttggatgcg ctcgaactgc ctgatttgtt
     4981 cggcgtggct gcgcgcgtcg gcatacgcgc ctgtgtgcag ctcgacaacg ggcgcgccga
     5041 catcacgggc ggcttggatt tgcctgtcgt cggcatcgat aaacaaggac acgcgtatgc
     5101 ccgcgtcggt caggattttg gtgaattcgg caattttgtc ttgttgcgcc aatacgtcca
     5161 agccgccttc ggtcgtgatt tcctgacgtt tttccggcac gatgcacacg tcttccggca
     5221 tcactttaag cgcgttttcg agcatttctt ccgtcaacgc catttcaagg ttcaggcgcg
     5281 tgcggatggc gtttttgaca gcaaacacgt ccgcgtcttt gatgtggcgg cggtcttcgc
     5341 gcaggtgcat ggtaatcagg tcggcaccgt gcgtttcggc aaccagtgcc gcctccacgg
     5401 ggctgggata agtcgtgccg cgcgcattgc ggacggtagc gatgtggtcg atgttgacac
     5461 ctaaaagcat aatctttcct tttatttctg ccttcagacg gcatttgaag ccgtgccgtc
     5521 cgaagtcggg acggtttccc gggcggtttc tttgcggtca aactgccgta tctgttccaa
     5581 cacctgccgc gatttcagcc cctcgggcaa caggtggcgg ataaaaagcc gtgtgatttt
     5641 caatgcctgt tgcaggcttt cggcagtgcg gaaactgcct tcgcgcaaat cgatcaggct
     5701 ctgcccgggg gcgacaacgc cggcggcgtg cggcggtacg gcaaatcctt ttccgacggg
     5761 gaagacggct gtttccgggc ggacaaggta tgtgccgcct gccgcaatcg tcccgccgtc
     5821 cccgtcgcgg ttcaaatcgg gggcaacgcc caacaggttc agcagccgcc actcgaaacg
     5881 gcgcaagtcg tcgatataag cggctttgca gcacaccgcc tccatcactt ccgccaacgc
     5941 gtcgtataac tcgggcaacg gatcttcgcg gacggtcaac ttcagcatca actcgttcac
     6001 atacaatccg ctgaacaacg ccctaccctg cggctgcggc cacccgccta tccattcggc
     6061 gcgatgtagg gtcttcaact cttgcgaacc ataccaagac gcactcaccg gcacaaacgg
     6121 caccaacacg ccgcgcaatt cgctctgccg tttgcgcgcg cttcttgcca aaagtgccac
     6181 acgcccgtaa cggcggctga acgcttcgac ccgcaggctg ctttcgcgcc agggcgagga
     6241 agccaacagg aagacgggtt cgtggttgac tcggtgttcg gacatcatgc ctgaaatgcc
     6301 gtctgaacgc tgccgccgct acaaaacggc ggtcgggtac gaaccgatga ctttgacgaa
     6361 cgaagcgcgt tcgcccaagc gttccaatgc cgtctgaatc tgcgcgtccc ggcggtgtcc
     6421 ttcgatgtcg atgaagaaca ggtattccca caaaacggat ttactcggac ggctctcaaa
     6481 cttggtcatg gaaatacccg actcagtcag cggttgcagc agcgaggcaa ccgcgcctgc
     6541 ccggttgggc gcggaaacgg ccagcgaagt cttgtcgctg ccgcttgcac cggtttcgtg
     6601 atgtcccatc accaagaagc gcgtggtgtt gttcggttcg tcttcgatgc actcggcaac
     6661 catatcgagt ccgtagattt ccgccgccgt gcgtccggcg atggcggcaa ccgtaccgtc
     6721 gtccgattcg gcaaccagcc ttgcggcttc ggcattgctg gacacggcaa tccgttcggc
     6781 gttgggcagg tgtctgccca accagtcgtt gcactgcgcc aacgcctgcg cgtgggaaaa
     6841 gactttggca atgccttcgg tgctgccgtt gtttttacgc aaaaggttgt ggtggatgcg
     6901 caaaacgact tcgccgcacg cctgcaacgc ggtaacggca agcaaatcca acgtgcgccc
     6961 gaccgagcct tcggtcgagt tttccacggg ggcgaccaga tagtctgcct gacgggtttc
     7021 aacctgcttg aagcagttgt ctatggtcgg acacgccatg gtgtgcgcgg cgtgtccgaa
     7081 atgcttaatc gccgcctgct gggtaaacgt gccctgcggc cccagatagg cgatggtcag
     7141 cgggcgttcg acggcgaggc actcgctcat cacttcccga aacaggcgtg ctaccgattc
     7201 gtcgggcagc gggcctttgt tcaaatcctg aatgcggcgc aacacggcga cctcgcgctc
     7261 cggacggtat actgcgcccg tgcctttcag ttcgccgatg gcgtgggcgt gttgcgcacg
     7321 ttcgttgagc aggcgcagga tttcggcatc gatggtgtcg atggcgttgc ggtggggaag
     7381 gaggagttcg tcgatagttt gggacataat ttcgcctgaa cagtaattta tcgtgcattc
     7441 taacatcaaa tgccatctga aacggtttca gacggcattt gatgttaaag gtattcgctc
     7501 tgttcgtttt ctttccacgc acgatgtgag cagagccaca gaagcgcaat gccgcaagag
     7561 gtggacgcgg tcatggttac cgccatcacg gttgccgaac cgttgtgcaa gaaggtcgcc
     7621 gccataccca cgcccgcgcc gattaaggac tggaacacgc ccaacacggc gttcgcgctg
     7681 ccgccctctt ctttgaaata agacataaag catgcctgcg tgttcgcgcc gaccaagccc
     7741 tgcgtgccga cggaaaacat cacgcacgca accagcagcc aaaacggggg caacccgaaa
     7801 aacagcacgg cggcgagttg ggacaggttg gcggcaaatt ggacgacaat cccccatagc
     7861 aggatgcttt gcggatgcac gccggttttg agccgccacg cggtaacgcg gttgaaaaac
     7921 atcatcgtga tgatgttgag tgcaaacgcc caagcgtatt gatgaggcgt aacacggtaa
     7981 agctgctggt acacgaagga agattcggtc agaaaggcga acatcgaacc gaagctgaat
     8041 gcctgaaaaa acagataacc catcgcggca cgggttttca atacgcgctt gaaccgcccc
     8101 gccaccagcc cgaacacgtc ccgtccgatt ttgccgccga cggcaggctt gggcaggaaa
     8161 tgctgcacca aaccgagcag caccagcgaa tacgccgcca gaaaaacaaa aatcgcctgc
     8221 cagccgccca agccctgcaa caatgcgccg accatgggtg cgaccagcgg cacaaccatc
     8281 aaaatgatgc cgataagggc aaacatctgg gcggctttgc gtccggaata ataatcgcgc
     8341 accatcgcgc cgacgatgac cacagtcatg cccgcaccga atgcctgcac gacacgcagg
     8401 ttgaggagct gttcggcact cgaagcaaat acgatggcgg caacggcaag gcaatataca
     8461 atcagaccgg tcagggcgac gggtttgcgc cctttgatgt cggacaccga accgccgacc
     8521 acctgtccga acgccgtgcc gaacataaac aaactcaggc tctgttcgat gcggtggaca
     8581 tccgcgttca gcgactgcgc catttcggga atcgcgggca ggtaggcatc gatggaaaac
     8641 ggcatcagcg caaccagcat cgccatcaaa accgccatca gtttttcgct catttcagga
     8701 taatgggcag aaggcataaa ttctcgtccg attcgcaaac aaaccgcccg gcggcggaaa
     8761 acacaatacg cggaaacgga aaattttagc acaccggcac aaacaggcgg caacaaaagc
     8821 cccggcaaaa ccggacgaac cggaacgata tagtggatta acaaaaacca g
//
LOCUS       DAWHXS010000081         8736 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWHXS010000081 DAWHXS010000000
VERSION     DAWHXS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.081"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(467..1804)
                     /gene="ribB"
                     /locus_tag="WIE22_001539"
     CDS             complement(467..1804)
                     /gene="ribB"
                     /locus_tag="WIE22_001539"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ4127606.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2026..3038)
                     /locus_tag="WIE22_001540"
                     /pseudo
     CDS             complement(2026..3038)
                     /locus_tag="WIE22_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(3359..3952)
                     /gene="ribA"
                     /locus_tag="WIE22_001541"
     CDS             complement(3359..3952)
                     /gene="ribA"
                     /locus_tag="WIE22_001541"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ4127607.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3945..4619)
                     /locus_tag="WIE22_001542"
     CDS             complement(3945..4619)
                     /locus_tag="WIE22_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127608.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMGMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPICTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            4773..4857
                     /locus_tag="WIE22_001543"
     tRNA            4773..4857
                     /locus_tag="WIE22_001543"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4807..4809,aa:Leu,seq:tag)
     gene            5377..6411
                     /gene="purM"
                     /locus_tag="WIE22_001544"
     CDS             5377..6411
                     /gene="purM"
                     /locus_tag="WIE22_001544"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ4127609.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(6885..7541)
                     /locus_tag="WIE22_001545"
     CDS             complement(6885..7541)
                     /locus_tag="WIE22_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ4127610.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPMLE
                     "
     gene            complement(7538..>8736)
                     /locus_tag="WIE22_001546"
     CDS             complement(7538..>8736)
                     /locus_tag="WIE22_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ4127611.1"
                     /translation="QALREGAERIGRRCFDIPVPEGGTPEFKQVGRCFNQMGGRLKIL
                     YDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQAAEHFLNRILPAVGADSG
                     RVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQNETLGRLLLSFPNGISLDE
                     DDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQGLHDSIAQALTFLNLQVQ
                     MLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRTKISNKEFPEAVADLFAR
                     FTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIRKHARATHVKFTLSEHGGR
                     FTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAVLEIRSQPQQGTTVSLTGA
                     SEESLK"
BASE COUNT         2071 a         2088 c         2311 g         2266 t
ORIGIN      
        1 atttagggga ttttggggaa ttttgcaaag gtctcagcct gttggcggct ttcaacaggt
       61 tgagaccttt gcaataacat aggttactaa aattttatgc tcaatctcat tttcaaaatg
      121 caaaactttt ctgatttttc ctactttttg ctcaatatta ggaaggtttt aggcaattga
      181 aaattttttg gcgcattttt atgcgtcaaa tttcgttaac agactatttt tgcaaaggtc
      241 tcatgccgtt ctttccctga aagagagaat ccaaaaaacc aaagccacag gaatttatcg
      301 gaaatgacaa aaacccgacg aaccggattc ccgcctgcgc gggaatgacg aattagaagt
      361 tacccgaaat ttgcaaaaac aaaatccaac ccaacagacc gggttttcgt ttgcacggaa
      421 ataatgcaat aaataaagca aatataaagt atttgaattt actatattat ttttccgctt
      481 cttcaaagcc gacgacttcc aaaccgaagc cggtcaggcc ggtgaaagat gagggctgcc
      541 cgaggacgcg cagttttttg acgttgaggc cggcgaggat ttgcgcgcct atgccgtagc
      601 ttttgctgtc ccatttgtag gcttggtttg cacctttggg cagggtgcgg tcgagcaggg
      661 cggcgccgtc ttcggtgcgg tgcagaagga tgactacgcc gctttcggcc tgttggatgc
      721 gctcgagtgc tttgggcagc gaccatgaat ggcgcgggtt ggcttggatg aagtccatga
      781 cgctgaaggg ttcgtggacg cggacgaggg tttcggtgtc ggcggagggc gtgcctttga
      841 cgagggcgag gtgggtttcg ccggagagtt tgtcgacgta aacgtgttgt tggaactcgc
      901 cccacggggt ttgtacaggc gcattgccca tgtcttcaag caggctttcg gtacggctgc
      961 ggtattcgat gaggtcggca atcgtgccga ttttgagctt gtgttcttcg gcgaatttca
     1021 tcagttcggg catacgcgcc atcgtgccgt cgtcgttgat gatttcgcaa ataacggagg
     1081 caggaatcag cccgttcatt tgcgccaggt cgacgccggc ttcggtgtgt ccggcgcgga
     1141 cgagtacgcc gcctttttgg gcgcgaagcg gaaagatatg accgggttgg acgatgtctt
     1201 cgggtttagc ggtcggggaa acggcggttt gaatagtcag ggcgcggtcg gcggcggaaa
     1261 tgccggtggt aatgccgtgt gcggcttcga tggagacggt aaagttggtg ccgtattgcg
     1321 cgccgttttt ttgggtcatc atcggcagcc cgagtttttc gaccatttcg ccgtccatcg
     1381 gcaggcagac caagccgcgc gcgtgtttga tcatgaagtt gatggcttcg ggcgtgacga
     1441 attgcgccgc catcagcagg tcgccttcgt tttctcggtc ttcggcatcg gtgatgatga
     1501 ccattttgcc ggctttgatg tcggctagga tttcggggat gggggaaata tgggacatgg
     1561 tgtgttttcc tgtgtgttcg ggcgggcggg cggtgtgttc ggtatcgagc cagaaggcgg
     1621 gcatttttgc cgcctgttcg attttacggg cttttttctc gccgaaggat ttgtttttca
     1681 aaagggcgga gagttcgcct gtgttgaggg caacggcttc ggcaaaacgg gtttgcaaac
     1741 cgccgtagtg ttctgctatc cattgccgca ggttgcggcg gcgcagaccg gctgtgtcgg
     1801 tcatcgtttc atcctttgta gaaatcggat gtagcggatt gtaatcgcgt atttaccaaa
     1861 aagcaaatct gtttttttcg ccaaaccgaa ttatttgctt tttggtaaac agatgccgtc
     1921 tgaagccgga ttcgtggctt cagacggcat tgccgcaatc cttgccggcc gggggctgtt
     1981 tgtcccaact tcccgcgcac ttctgatttg gggtttgccg gaagcttatt ccgaaaaaaa
     2041 tttctgataa acggccttga ttgccgggta cagcataggc ggtacgagca ggcgcaatat
     2101 cggcaatcct atgatgaatg cggtcttccc gataaaaacc ggacggattg tcttcgcgcc
     2161 gaatatcttt ctgcattttt ctttaaatga aaaatatttc cggtagatga ggataatgtt
     2221 tttggaaaaa taaagcctta ccaatataga cctcagatag ccgatattgt cgaagacaaa
     2281 tttttgcaaa ccgccgtaga tgggggaatt tttgttttcg actaaaaaat ccacaccccg
     2341 gtcgagatgc gtcagcacat cgctgaaatt tttgtatttg atgttgtggc tgatagagcc
     2401 gccgagaacg cggtactggt aaaaagggtt atcgtaaaag gcaaaagtct tgataaaacg
     2461 cgccaattgc aaactgtacg ggaaatcctc gtgaatgtat ccttttggga aaaacagatt
     2521 gtttttgatg attatttccc gcctgacgat ttttgtccac gcgttggcga tatagtaccg
     2581 cccctccacc agcgtttcaa aatggcggac aaaatcatta tccgaaaagt ccgccccttt
     2641 ggggatgacg tggtaattga gggacgaggg atgcaggatc aaatcaacct ttttgtctgc
     2701 aagttgttgt aaatcaaaga gaatcccccc cccgcgtttt ttgaacggtt ggtatcggcc
     2761 caatagtcgt cgctgtccaa aaagattagg taatcgcctt ttgccgcccg gataccggcg
     2821 ttgcgggcat ccgacagccc gccgttttct tgatgaatca cttttatatg cggatatttg
     2881 cctgcatatt cgtcgcaaat cttcccgcag ccgtccggcg aaccgtcatc gaccaaaatc
     2941 atttcataat cggcaaaatt ttcggcaagc acggaatcca cgcagcagcg gaggtatttt
     3001 tccacattgt aaatagggac gatgatggag aaaatcataa atatcaatcc attatattaa
     3061 gatgtttgcg cgtatgcctc aaacccgcgc tcgcaatgcg tttgcatccg caccctgtaa
     3121 ctttatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
     3181 agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa
     3241 ggcgaggcaa cgctgtactg gtttttgtta atccactata aaacttattg ctttatcaag
     3301 gtatggaaac ctgtttcccg aaaggcggcg caggatgccc gttccctgca actttgcctt
     3361 attccgacat cagatgtccc agcttgtctg ctttggtttg gaggtagcgt ttgttttcaa
     3421 ggttttcccc gacgtgcagg gggatgcgtt cgaccacgtt aatccccgca tctttcaggg
     3481 tttggatttt ttcggggttg ttggtcagca gtttgaccga gcggatgccc agatattcgt
     3541 agatagattg cgccaaacgg aaatcgcggg catcgacggg cagcccgagt gccaaattgg
     3601 cttcaaccgt atccatacct tggtcttgca gatgataggc gcggattttg ttaatcagcc
     3661 cgatgccgcg tccttcctga cgcagataga cgatgatgcc gcgcccctct gtctgtaccg
     3721 ccctcatggc cgcttcaagt tgaggtccgc agtcgcattt tctcgagaac agcgcatcgc
     3781 ccgtcaggca ttcggagtgg atgcgcgtca gaaccggatt gccgtctgaa aaatcaccga
     3841 cggtcagcgc gacgtgttcc tgcccgtttg cctcttcaaa gccgtgcatc gtaaatacgc
     3901 cccattcggt cggcaggcgg caggaagcaa catagtttaa taattcagac attttcatct
     3961 ccttctgcgg cagtgatgcc caaaagctgc ttcaaaggtt cggacaaagc caaggcaaga
     4021 gccacccatt ccgcctgcgc tgcctcatcc gcacactctg cgcattcaaa ttccacatga
     4081 accacgccca acacaccgcc actttccgtg cagattggaa tggaaatttg cgccgccgaa
     4141 gcatgattgc gttctcccga aagctccccc aaatccaacc aacggcgtac atccgaggca
     4201 acattcatcc agccgctttg cgcgcaacgg caggcaagtg aaacatcggc aacattttca
     4261 tctagattcc atatgttttc caaaccttct ccctgttggg acaggcatat cagttggaaa
     4321 gcctggtttt cagacggcat caaggcatag accgccgcac tccgcacgcc tgtcgagcgc
     4381 gaaaacaccg aatccaaagc catgaaaagc cgtttcagtg catcttcgcg gacattgtcg
     4441 cacgacaggt aatcggcaag tttccaaccc tcatcactgc gccacaaaac cgaacggtct
     4501 atcgaagccg tccccatacc cattaccgtc tgcgccgtca gatacgccgc ccgaacctcg
     4561 tcaagcggca gcttcaaacc ctgagtcagc agaaaatcct taatcaaagc agcaggcata
     4621 cccaaattcc gtcaataaaa acaaaaaacc gcccgattcc gatgtcaggc ggacgaaaaa
     4681 cagattttac cgccccaaac cccaagccat caacagcccc gccgaaaacc ttgcacaaaa
     4741 aaacaaaatc tgtataatcg tccaattccc atgcggacgt ggcgaaattg gtagacgcac
     4801 cagatttagg ttctggcgcc gagaggtgtg agagttcgag tctctccgtc cgcaccaata
     4861 atgataaata aattatatag ttaatgctta tcgggacaaa ttagtgccac aaaacataag
     4921 tcgtctgctt ttcagcgggc ggctttttta tctgcctata tgtttcaaca cgcagaacgg
     4981 tacataaagt gccgccattt gttccgcacc gaatcggaaa cgggtgcttc cgcccgcaat
     5041 cagattcacc gcccctaatg ccggggtttc aaccggaaag aagaaaatat tgattttgat
     5101 gatggcatca aaaaacccct tccgctcgtc tgccaaaagg aatggttaag aaaagcaatg
     5161 cgtcttttga tggaacgcga tatataagga attgagggga agggcaggcg ggtttctgcc
     5221 cgttaatcct gcggacggtc ccgccaaacc cgacacgcgc gggtatatgg cggactgttt
     5281 acaatattgt ctaaaaatct gaacctgcaa acgtgaaata aggtagaata cgccccgtta
     5341 ttttaccgtc caatccccat ttaccaagga aaaacgatga gtacttcatt gagttaccgc
     5401 gatgcaggtg tcgatatcga cgcaggcgac caactggtcg aaaacatcaa accgtttgcc
     5461 aaacgcacca tgcgtccgga agtattgggg gatttgggcg gttttggcgc attggtcgaa
     5521 atcggcaaga aatataaaaa tcccgtattg gtcagcggta cggacggcgt gggtaccaag
     5581 ctcaaacttg ccttcgattg ggataaacac gatacggtgg gcatcgacct tgttgcaatg
     5641 agtgtcaacg atattttggt tcaaggagca gaacctctgt ttttcttgga ttatttcgcc
     5701 tgcggcaaat tggatgttcc gcgcgcaacc gatgtcatca aaggcattgc acaaggttgc
     5761 gaagaatccg gctgcgccct gattggcggg gaaactgccg aaatgccggg tatgtatccc
     5821 gtcggagaat acgatttggc gggttttgcc gtcggcgtag tggaaaaaga gaatgtcatt
     5881 accggccgca gcatcggcgc gggcgatgtg gtattgggtt tggcttccaa cggcgcacat
     5941 tcaaacggct attcccttat ccgtaaaatc atcgaacgcg acaatcccga tttggatgcc
     6001 gagtttgata atggcaaaac cttgcgtgag gctgttattg cgccgacccg tctgtatgtg
     6061 aaaccgattc ttgccgcttt ggaaaaattt accattaaag gtatggcaca cattaccggc
     6121 ggcggcatta ccgaaaacgt gccgcgcgtg ttgcctgaaa acacggttgc acaaatcgat
     6181 gctaaatcgt gggaattgcc caagctcttc caatggcttc aaaaggcggg caatgtggaa
     6241 acccaagaaa tgtaccgaac ctttaactgc ggcatcggca tggtcgttat tgttgctgcc
     6301 gaagatgccg atgcggttca gggtctcttg ggtgaacaag gtgaaacagt ttaccgttta
     6361 ggtttgatcc gtgagcgtca gggagacgag catcaaaccc aggttgcctg attgcttcta
     6421 tagcgaaatg ccgtctcaaa catgggtttg gacggcattt ttatagtgga ttaacaaaaa
     6481 tcaggacaag gcgacgaagc cgcagacagt acagatagta cggcaaggcg aggcaacgcc
     6541 gtactggttt ttgttaatcc actatatatt gattgatatg agggtgggtt acacatgctt
     6601 aaacgatata agccattgta tatttatggg attcggaatg gaacatttta ccctgcatta
     6661 gtatttccgc tttttattat gccgtctgaa aatatcatga tgatgcgttt tcagacggca
     6721 tagctgatat tgcagtatca gataggggca aacggaaaac actgaaccga acaatattac
     6781 tgctatgcca agcgtactta tgggggcata aacttacatg cttggtctgt tatccgaatg
     6841 accgtacggg gattaaaacc cctaatatgc cgtctgaacc cgccttattc cagcatgggt
     6901 tgggggacgt tgtgccggat ggcgtaaaca gcggcctgca cccggctgct gaggttgagt
     6961 ttgcggagca ggttttgaac gtggactttg acggtggatt cggcaagatc gaggtggcgg
     7021 gcgatgattt tgttgctgtg ccctgcggcg agatagccca agatttccag ttcgcgaggg
     7081 gtaagtgagg agagtgcctg tgtcccttgg gcaggttggg gggaaatcag gcttttaacg
     7141 agtttggcgg tcatctcggg cgagaataca ttatcgcctt cggcggcttt gcgtatgctt
     7201 tcgagcagaa agtcagcgtt gatgtttttc agcaggtaac cgcgcgcgcc gatgcgaata
     7261 cattcggtca agtcgtcgct gtcttcggaa acggtcagca taatcactgc ctgctgcgga
     7321 ttgatgctga tgatttggga gagtgcttcg cgtccgttca tgccgggcat atccaaatcg
     7381 agcaggacga catcgggttg cagccgacta atcattttga tgcccgagag gccgtctgcg
     7441 gcttcgccga tgacttcaaa accgtgttgg cgcgacaaaa gggctttaat gccgctgcgg
     7501 aagagggtgt ggtcgtctat cagaataatt ttaatggtca tttcaagctt tcttcagatg
     7561 cacccgtcaa tgagacggtg gttccctgtt ggggttggga acggatttct aaaacggcat
     7621 ggatgcgctt ggcacgctcc tgcatgatgt gcagtccgac atggctgccc gtaggttctc
     7681 ctattttctc cgtgtcgaaa ccttgtccgt tgtcttggat ggtcatggta aagcgtccgc
     7741 cgtgttcgga aagggtgaat tttacatggg tggcgcgggc gtgtttgcgg atgttggaca
     7801 ggctttcctg caggataaaa atcatttgga gctgcgcttc ctgaggcggc aggaacgaac
     7861 cgttttccca ggcggtttcg accgttatcc cggtttgttg cgtaaagcgg gcgaataggt
     7921 cggcaacggc ttcgggaaat tctttattgc tgattttggt acggaagttg agcagcagtt
     7981 cgcggacatc ttcataacat tcctgcacgc ctgttttgat gaagccgatg ttttctgcgg
     8041 cttcctcccg tttgttttcg gcaaaggcgg tttccagcat ctgtacctgt aggtttagga
     8101 acgttaatgc ttgtgcgatg ctgtcatgta atccttgcgc aatcaggttg cgttcctgca
     8161 atactgcaag caagcgtttt tcttcctcct gttttgcgcc ggcaagcgat acgcccaatt
     8221 gcctgcctag tgtttgaagc aggatgcggt cgtcttcatc aagagaaatg ccgtttggaa
     8281 agctgagcaa cagcctgccc aatgtttcgt tctggtactc aatggggaag atttcctcat
     8341 ggtacttccc caaatccgaa gctgctgtgc cgcaatccgc atgatgaatg gaaacataaa
     8401 catcggatcc gccgtccaaa caaactctgc cggaatctgc tcctacggcg ggcaggatac
     8461 ggtttagaaa atgttctgca gcctgttgcg gtatgtagga ttggtgcagg tcccgtgtag
     8521 tttggtacag cagggtcagg ttttgatttt gtttttcgag actgcgtgtc tgctcggcga
     8581 cttgtccttc caaatcatca tataaaattt tcaacctgcc gcccatttga ttgaaacaac
     8641 gcccgacctg tttgaattcc ggcgtaccgc cttcgggaac cggaatatcg aaacacctcc
     8701 gtccgatgcg ttccgcacct tcccttaacg cctgca
//
LOCUS       DAWHXS010000082         8467 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWHXS010000082 DAWHXS010000000
VERSION     DAWHXS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.082"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WIE22_001547"
     CDS             complement(207..1592)
                     /gene="pntB"
                     /locus_tag="WIE22_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ4127612.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHSVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1658..2041)
                     /locus_tag="WIE22_001548"
     CDS             complement(1658..2041)
                     /locus_tag="WIE22_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ4127613.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2045..3586)
                     /locus_tag="WIE22_001549"
     CDS             complement(2045..3586)
                     /locus_tag="WIE22_001549"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ4127614.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            3876..4709
                     /gene="serB"
                     /locus_tag="WIE22_001550"
     CDS             3876..4709
                     /gene="serB"
                     /locus_tag="WIE22_001550"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ4127615.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            4773..5996
                     /locus_tag="WIE22_001551"
                     /pseudo
     CDS             4773..5996
                     /locus_tag="WIE22_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6160..7740
                     /gene="purH"
                     /locus_tag="WIE22_001552"
     CDS             6160..7740
                     /gene="purH"
                     /locus_tag="WIE22_001552"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ4127616.1"
                     /translation="MPSIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLPEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDPADFPAIAAEME
                     ANGGALSDKTRFNLSRKAFSHTAEYDGMISNYLTSLSDDVLSGQPQIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIYPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAIASNTLDAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
     gene            8353..>8467
                     /locus_tag="WIE22_001553"
     CDS             8353..>8467
                     /locus_tag="WIE22_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ4127617.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIE"
BASE COUNT         1887 a         2323 c         2375 g         1882 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 tcactgcaaa ccttcataaa cgaggtttgc agtgattttt tcaaatcaaa cagattgaaa
      181 acctgcgccg aattattcag acggcattat tttttcagtt cggacagaat gtcatctacg
      241 gttttcttcg catctccgaa acacattacg ctgttttcgt tgaagaacag cgggttttgt
      301 acacctgcgt agccggtatt catcgagcgt ttgaagacga cgacttcttt tgccttccat
      361 acttccaaca cgggcatacc cgcaatcggg ctgttcgggt cggtttgggc ggcaggattg
      421 acggtatcgt tcgcaccaat caccaaaacc acatcggttt cggggaagtc gtcgttgatt
      481 tcgtccattt ccaaaacgat gtcgtagggg actttggctt cggcgagcag tacgttcata
      541 tgaccgggca ggcggccggc gacggagtgg atgccgaagc gtacttcggt gccgttttta
      601 cgtaaaagct cggtgatttc ggcaacgggg tattgcgctt gtgcgactgc cataccgtag
      661 cccggggtaa tgatgacatt gtttgcgcct ttcagcattt cggcaatatc ggcagctttg
      721 acttctcggt attcccctat ctcttggctg ccggaagata atgtgccgct gtcgctgccg
      781 aaaccgccgg caattaccga gacaaacgag cggttcatgg ctttgcacat aatgtaggac
      841 agaatcgcgc cgcttgagcc gaccagcgcg ccggtaacga tgagcaggtc gttggagagc
      901 atgaagcctg ccgctgcggc cgcccagccg gagtaggagt tgagcatgga cacgactacg
      961 ggcatatctg cgccgccgat ggaggcaacc aagtgccagc cgaatgcgag ggcaatcagg
     1021 gtcataatca gcaggatgaa gccgctaccg ttaatgccga cgaatacgag cagcaacaca
     1081 aacgatacgg cgagcgccag tgcgttgagc ttgtgtttgg cgggcagttg cagcgggctg
     1141 ctgctgattt tgccgttgag tttgccgaat gcgaccagcg agccggtaaa ggttaccgcg
     1201 ccgatgaaaa tgcccaaata gacttcgacc agatggatgg tgtgcatatc gtgcgaaacg
     1261 ttgcccggct cgatatagct gttgaagccg accaaaaccg ccgctaggcc gacgaagctg
     1321 tgcagcaggg caatcagttc gggcatttcg gtcatttcca cctttttggc tttgtagatg
     1381 ccgattgccg cgccgatgag catggcgatg atgatccagc ccagtccgtg ggtattgtcg
     1441 gaaaaaacag ttacaaaaag ggcgaccgcc ataccggcga taccggaata gcagccctgt
     1501 ttggcggttt cctgtttgga cagccccgcc agtgagaaga tgaataaaat tgcggcaacg
     1561 atatacgccg ctgttacgag tcctgaagac atggaaattc tccgattttc gatgatttgt
     1621 ttttaatgcc gtctgaactg ttcagacggc atctgtttca agcagccgcg acaaacagcc
     1681 cgacggtgca ggcaaatccg cccgcccaca tcagcgagcg caatgctgct ttgtctgcga
     1741 tgtagcacca gataaaggcg aggcggaaca ggatgaacag gccggcaagc gtgttgacgg
     1801 ttgcttgtcc ggcattgccg gttgcgtgtg ccgtcaaaac ggcggcggca aacggtgcaa
     1861 aagcttcaaa accgttttgc tgcgcagcgt gggcacgggc ggctgcgcct tgcgtgtgcg
     1921 ccagaaaatc gcgcggattg tggttgtctt taaaccggaa tccgcccgct tttttggcat
     1981 atgccgcaca aaaaagcggc aataggcagg caatcaggat gcaccaatag gcgaaagtca
     2041 tggcttaccc tttcttaaac atattcagca tacgccgtgt aaccgcaaag ccgccgaaga
     2101 tgttgatgcc ggcaatcagg atggcaacaa acgacagcag cgaaacaaag ccgttgccct
     2161 gaccgatttg cagcagcgcg ccgacgacga tgatgccgga gatggcgttg gtcacggaca
     2221 tcagcggcgt atgcagcgag tggctgacat tccaaacgac gtagtagccg atgacgcagg
     2281 cgaggacgaa cacgataaag tggttcaaga atgctgcggg tgcgaccgcg ccgacccaca
     2341 gcactaatac ggcggcgatg acggcaggcg cgagtttttt ccacagggga acgggtttcg
     2401 gctcgggctt ggcggcaggc gcggcttttt cagacggcgt ttgctgcggc tgggcggaaa
     2461 cttgaatcgg cggaggcggg aaggtgattt cgccgtcgcg ggtaacggtc atgttacgga
     2521 taatcacgtc ttcaaaatcc agcgtgattt cgccgtcttt gttcgggctt aacagcttgg
     2581 tcaggttcac caagttggtg gcgtaaagtt gggaagactg tccggcaagg cggtttgcca
     2641 tgtcggtgta gccgatgatt ttcacgccgt tgccggttac ggacaattcg cccgggcggg
     2701 tgagttcgca gttgccgccc gtcgccgccg ccaaatcgac gatgacggag ccggatttca
     2761 tgctttccac catttctttg gtaatcagct tgggcgcggg tttgcccgga atggcggcgg
     2821 tagtgatgat gatgtccact tctttcgcct gctcggcaaa gagtttcatc tcggcggcga
     2881 taaattcgtc gctcatcact ttggcgtagc cgtcgccgct gccgcccgat tcttgcggga
     2941 agtcgagttt caggaacttg ccgcccatcg attcgatttg ttccgccact tccaagcggg
     3001 tatcgaacgc gcgcaccact gcgcctagtg aatttgccgt accgattgcc gccaaacctg
     3061 ccacacctgc gccaatcacc aaaacctgcg cgggcggcac tttgccggcg gcggtgattt
     3121 ggccggtgaa gaaacggccg aaggcgttgg cggcttcaat tacggcgcgg tagccgctga
     3181 tgtttgccat cgaagacaaa gcgtccaaag cctgcgcgcg cgaaatgcgg ggcaccatgt
     3241 ccatcgccag cgcgttcact ttcttggcgc gcaaggcttc gaccaaagcc tcgttttggc
     3301 gcggccacag gaagctgacg atggtttgac cttcgttcaa aagcggcagc tcgccttcgg
     3361 acggcgcatt gaccttataa atcaaagggc aggcccaaac cgccgctttg tcggcaacgg
     3421 tcgcgcctgc tgtttggtaa gcggcatcgt ccaaacttgc cgccaaacct gcaccgcttt
     3481 cgacaacggt ttcaaagccc agtttgccca gcagggcgac ggtggcgggc gtacaggcga
     3541 cgcgggtttc gccggataat gactcgcgtg ggataccgat tttcatctct gaatcctttt
     3601 tcgggtagtt tatatgtatc gtgggttaaa tttaaatcgg ggcagggcgg agcaacgccg
     3661 taccggttta aaaccgattc acttcaaatg ttaatatatt ttagataatc cccttataac
     3721 gaattttcat caggctggca atagttgcgg cattttcccg tgttgtccga cacatactgc
     3781 cgtttgcgtt tgaagtcatt ttccgattgc agtccctccc gccgtaacat tctttgccgt
     3841 ttcactatat aatccgcatt ttttgagccg tcgttatgcc gcacgccctc gtcctccaat
     3901 ttccctccgc cgaagccctg ccttccgact tcccctcccg cctgcccgaa cctgattgcg
     3961 ccgatgaaaa gcgtatgcgt tttatcgttg aagaagggtt ttctttaagc ggggcggacg
     4021 cggcgttgct tggcagccgt caaatcgacc acgccgtgtt gccggatatg gattttggcg
     4081 aactcggttt gattgtcagc gatatggatt cgacgctgat taccatcgaa tgcgtcgatg
     4141 aaattgcggc aggcgtgggt ttaaaagaca aggtagcgga aattaccgag cgttcgatgc
     4201 gcggagagtt ggatttcgag cagtcgttgc gcagccgcgt tgcgctgttg gcgggattgg
     4261 acgaacagat tttggcggac gtttatgaaa acgttttgaa gctctcgccc ggtgcggaat
     4321 ttttgttgga cgaatgcaaa aggcacgatg tgaaattcct gctggtgtcc ggcggcttca
     4381 cgttttttac cgaacgtctg caacaacgcc tcggcttcga ataccaacac gccaatgttt
     4441 tggaaattga aaacggcagg ttgaccggcc gtctgaaagg cagaatcatc gacgcgcagg
     4501 caaaggcgga tttattgcgc gaataccgca gccgcctcgg attgcagcca catcaggtgt
     4561 tggcgatggg cgacggtgcg aacgatattc cgatgctcaa agaagcgggc ataggcgtgg
     4621 cttaccgtgc caaaccgaaa gcgcgggccg ccgccgatgc ctgtatcaat ttcggcggtt
     4681 tggagcgtgt acgcggcctg ttcggatagg cggataggaa acggatgtcg tccgaaaggt
     4741 tttcagacgg catttgaacg gcaggaacga cagtgggacg cagaaaactt tggtttgccc
     4801 tggcagcagc aggcgttatc ggcggtttgg tcggcattgt gctgacggaa ctgatgcact
     4861 tcatacagca tacggcatac ggttatggcg cggacggtgc gtacatctcg ttccgcgaag
     4921 gtgtggcccg tgcttcggaa gggcggcgtt taggcgtatt gattctgtgc ggtatgttgg
     4981 caggcggcgg ctggtggttg ctcaaacgtt tcggtaagcc gcaaatcgaa attaaagccg
     5041 ccttgaagca gccgttgcag gggctgccgt ttctgacgac ggttttccat gttctgctgc
     5101 aaatcataac ggtcggactc ggttcaccgc tcggacggga agtcgccccg cgtgaaatga
     5161 ctgccgcgtt tgcttttgcc ggcggcaggc ggttgggttt ggacgaagac gaaatgcggc
     5221 tgctgattgc ttgcgcctcg ggcgcgggtt tggcggcggt gtataacgtg ccgctcgcct
     5281 ccacgctgtt tatcctcgaa gccatgctgg gcgtgtagac gcagcaagcc gccgccgccg
     5341 cattgctgac ttcggtcatt gccaccaccg tcgcgcgcat cggcttaggc gacgtgcagc
     5401 aatatcatcc cgccaacctt gccgtaaaca cttcattact ttggttttcc gccgtcatcg
     5461 gcccgatact gggcgcaacc gccgtctttt tccagcgcac cgcccaaaaa ttccccttca
     5521 tcaagcgcga caacatcaaa atcattccct tggcagtctg catgtttgcg ctcatcggcg
     5581 tgattgccgt ttggtttccc gaaattttgg gcaacggcaa agcaggcaac caactgacct
     5641 ttggcggttt gaccggttgg caacacagcc ttgagctgac cgccgtcaaa tggctggttg
     5701 tcttgatggc gttggcggca ggcgcatacg gcggactgat tacgccgtct atgatgctcg
     5761 gcagcaccat ttcttttgca gccgccgccg cgtggaacag cgttttcccc gaaatgccgt
     5821 ctggaagcgc ggcagtcgtc ggcgccgccg ttttcctcgg cgtttccctc aatatgcctc
     5881 tgaccgccgc cgtctttgtt ttggaactca cctacgcccc cgccgctttg ttgatgcctt
     5941 tatgtacggg tatggcgggc gcggtatgga cggcaaggaa aatgggatgt aaataaccga
     6001 aggcaaaatg ccgtctgaaa ccggttttca gacggcattc ctttggggat tgtcaacaat
     6061 atctgcaaaa acctactgcc ggaaagtgga aaatagcgga taataccgcc ccgacaacat
     6121 taccgtttcc catcccaacg tcagaaaaag gaaatcccta tgccttccat caaacgcgcc
     6181 ctgatcagcc tgtccgacaa gacgggcgca gtcgaatttg cccaaaccct gcacaagctc
     6241 ggtgtcgaaa tcctctctac cggcggcacg gcgaagctgc ttgccgatgc gggcgttccc
     6301 gtgattgaag ttgccgacta caccggtttc cccgaaatgc tcgacggccg cgtgaaaacg
     6361 ctgcatccga aaatccacgg cggtattttg ggccggcgcg atttgcctga acacgtcgcc
     6421 aagatggaag aacacggcat cggcaacatc gacctcgtgt gcgtcaacct ttaccccttc
     6481 gccgccacca tcgccaaacc aaactgcaca ctggaagacg cgattgaaaa catcgacatc
     6541 ggcggcccga ctatggtgcg ctccgccgcg aaaaactgga aacacgtcgc catcgttacc
     6601 gaccccgccg acttccccgc catcgccgcc gaaatggaag ccaacggcgg cgcattgagc
     6661 gacaaaaccc gtttcaacct ttcgcgcaaa gcgttcagcc acaccgccga atacgacggc
     6721 atgatttcca actacctgac ctcgctttca gacgacgtct tgagcggcca gccccaaatc
     6781 ggcgaatttc cgagccggtt caaccaaagc tggattaaag tacaagacat gcgctacggc
     6841 gaaaacccgc accagcgcgc cgcattctac cgcgatattt accccgccgc aggcagcctt
     6901 tccgcctaca aacagctgca aggcaaagaa ttgtcttaca acaacatcgc cgatgccgat
     6961 gccgcatggg aagccgtcaa atcctttgat gcgccggcct gcgtgatcgt gaaacacgcc
     7021 aatccgtgcg gcgtagccat cgcctccaat accttggatg cctacaaact cgcctacgcg
     7081 accgacacca ccagcgcatt cggcggcatc atcgccttca accgcgaagt ggacggcgaa
     7141 accgtcaaac aaattaccga caaccaattt atggaagtcc tcatggcgcc gaagtttacc
     7201 gccgaagccc tcgaaatcgc cgccgccaag aaaaacgtgc gcgtgttgga agtgcctttg
     7261 gaagcaggcg cgaaccgctt tgaactcaaa cgcgtcggcg gcggattgtt ggtacaaacg
     7321 cccgacatcc accgcatcag ccgcgccgat ttgaaagtcg tctccaaacg ccaaccgacc
     7381 gagcaggaat ggaacgattt gctgttcgtc tggaacgtcg caaaatacgt caaatccaac
     7441 gccatcgtgt tcggcaaagg cggacaaacc tacggcatcg gtgcaggcca aatgagccgc
     7501 gtggacagca cccgcatcgc cgcccgcaaa gcgcaggacg ccaatttcga cctcaacggc
     7561 gcgtgtgccg cctccgatgc cttcttcccc ttccgcgacg gcgtggatgt gattgccgaa
     7621 cagggcatta aagccatcat ccatccggca ggttcgatgc gcgatcaaga agtcttcgac
     7681 gcggcagacg aacacggcat tgcgatggtg gtaaccggca tccgccactt ccgccattaa
     7741 ggcagatgaa caaggcaatg ccgtctgaag ggctttcaga cggcattttg cgctattttg
     7801 tgaaggtagg gatgacggtt cgggtattcc tgacagggtg gattttcaag gtgttgtata
     7861 gggtgtagga ggattcgtaa aaggtgggat gcagggtgtg cttcagcccg ctgcatcaaa
     7921 aatttttgga gaaccggcgg gagtcggcgg ttttggtttc ggcggggacg gtggaaatgg
     7981 gtaacattga cggaatcgac ggaagcggtg ggctgaagcc cacccttgta tattggaatc
     8041 actgtatcat agcaacaaac cgcccggccg ccacccgcgc ccacccaagg cacacaaccg
     8101 ttgcgtagct cagggagcgg cagggcaacc catcgacaca accggacagt tgccggacaa
     8161 cacaaccgaa tgcaaggcag gtttatgatg agtacccaat accattacgc aggtatagtg
     8221 aattaaatct aaaccagtac agcgttggtt cgccttagct caaagagaac gattctctaa
     8281 ggtgctgaag caccaagtga gtcggttccg tactatccgt actgtctgcg gctcgccgcc
     8341 ttgtcctgat ttttgttaat tcactatatc gacatcgcca aacgaaactt cgtcatcgcc
     8401 gtttcgtctt tgtctaaaac caaaaccgaa accaacaacc ccaaaggtat cgcccatact
     8461 atcgaat
//
LOCUS       DAWHXS010000083         8186 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWHXS010000083 DAWHXS010000000
VERSION     DAWHXS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.083"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(309..2345)
                     /locus_tag="WIE22_001554"
     CDS             complement(309..2345)
                     /locus_tag="WIE22_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ4127618.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLACRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDSVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVVRPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2433..3305)
                     /locus_tag="WIE22_001555"
     CDS             complement(2433..3305)
                     /locus_tag="WIE22_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ4127619.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3575..5965
                     /gene="gyrB"
                     /locus_tag="WIE22_001556"
     CDS             3575..5965
                     /gene="gyrB"
                     /locus_tag="WIE22_001556"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ4127620.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASAETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6062..7447
                     /locus_tag="WIE22_001557"
     CDS             6062..7447
                     /locus_tag="WIE22_001557"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ4127621.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVADVMEGCIK
                     RGLAADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTALTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKSTYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7518..7616)
                     /locus_tag="WIE22_001558"
     CDS             complement(7518..7616)
                     /locus_tag="WIE22_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ4127622.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         1903 a         2244 c         2273 g         1766 t
ORIGIN      
        1 ccttgccctg atttttgtta atccactata aaatgccgtc tgaagcgttg tttgaccgtt
       61 tcagacggca tttttatcaa attcaaaaat attccgtcat tcccgcgaaa gcgggaatct
      121 agaacgtaaa atctaaagaa accgttttat ccgataagtt tccgcaccga cagacctgga
      181 ttcccgcctg cgcgggaatg acgggatttt ggcttactgt ttttgtgaaa atggcgaaac
      241 cgtgcaaatg ctgatgccgt ctgaaacact gtttgattgt ttcagacggc attttttgtt
      301 cacatcgatc aagccgcgtt gtcgaaaccg ctgtggcgca agagtgcgtc tatgcgcggt
      361 tcgcgtccgc ggaaggcttt gaaggattct gccgcgctgc gcgatccgcc gacggcgagg
      421 atttcctgcc aaaagcgttt gcctgtggcg gcgacatcgt cgctttcttc aaaggcggcg
      481 tatgcgtccg cgctcaatac ttccgcccat gcgtagctgt aatagcctgc ggagtagccg
      541 ccggcgaaga tgtggccgaa gctgttggcg aagcggttgt attcgggcgg tcggacgacg
      601 gcgacttctt tgcgcacgct gtctaaaacc tgttgccagt ttttcagacg gccttcgtcg
      661 tcttcgctgt aaatcatcat atcgaagagg gcgaactcca tctggcggac gaggaacatt
      721 ccgcgttgga agtttttggc ggcgagcatt ttgtcgaaga gttctttcgg caggggctcg
      781 ccggtttctt cgtgggtgga catttgcgcc aatacattgt attcccaaac gaaattttcc
      841 ataaactggc tgggcagttc gaccgagtcc cattctacgc cgttgatgcc ggacacgccc
      901 agttcgtcca cttgggtaag caggtggtgc aggccgtggc cggtttcgtg gaagagggtg
      961 aggatttcgt cgtggctcag gcgggcttcc ctgccgccga cgggtggggc gaagttgcag
     1021 acgaggtagg cggtgggcag ttgcagcgtg ccgtctgaaa aacggcggcg acctttgtag
     1081 tcgttcatcc acgcgccgcc gcgtttgcct tcgcgggcgt agaggtccat atagacgccg
     1141 cctatggttt cgccgttttg ttgcaattca aaatagcgca cgtctttgtg ccagacgggg
     1201 acggtttttt cggtaaatcc gatgccgtag agttttttga tttgggcaaa cagtccgttt
     1261 aatactttgc cgacggggaa gtattttttg acttcggttt cgctgaacgc gtatttggct
     1321 tcgcgcagtt tttcgctggc gtagcccaag tcccacggtt gcaaatcggc gaggttcagg
     1381 ctttcgcggg cgaaggcttt gacttcggcg aggtcttttt cggcgtaggg tttggcgcgg
     1441 caggcgaggt cgtgcaggaa gtttaaaact tgttcggggg tgtccgccat tttggttgcc
     1501 agcgacaatt cggcgtagtt tttgaagccg agcagtttgg cggtttgcag ggcgtttgcg
     1561 agcgtgcggt cgatgttggc ggtgttgtcg aatttgccgc cgtctgaaag ttcgctggcg
     1621 cgggtaacgt aggcgcggta gatttgttcg cgcagttcgc ggttgtcggc gtattggatg
     1681 acggcgaggt agtgcggaat ctgcaaaccg attttgtagc ctgttttgcc ttcgctttgc
     1741 gcggcagcgg caaacatggc gagcgcgtct tcgggaatgc cggcaagcgg tgcagcatcg
     1801 tcaaagtaaa tgccgaacgc gtcggtcgcg tctaggacgt tttgggagaa tttggcggaa
     1861 agttgcgcgc cttcggtttg cagttttgcc agttctgcct gctgttcggg cggcagttcc
     1921 gcgccgctga ggacgaaatc gcgcagatcg tggttgagtt tggttttttg cgcgggggag
     1981 agggtgtcga actcggggga gtttttgatg gttttgaagc ggttgtacag ctcgatgtct
     2041 tgtccgattt cggtgaagaa gacggtgatt tcgggcatca gttcgttata gacggcgcgc
     2101 agttcgggcg tgtcggcgac ggagttgagg tgcgacacca cgccccaaat cctgccgacg
     2161 cgttcggtga tgccggtcag gggttcgaca gtgtttgccc agccggtgtg cgtttgggct
     2221 ttgatggcgg cgatttgttc gcgcgcttcg gcgatggcgg tttgcagggc gggtttgatg
     2281 tcttcggttt tgatttgatc aaaacggggt tcttcgccca aatggagcag tgcgttgtca
     2341 gtcataagat gggtttcctt tgcgtgttgt gcttcagaca gcatatcggc aatatgccgt
     2401 ctgaagccgg aaatggggtc ggtcggggaa agttaaagtg tttcggagcc ggcggggtaa
     2461 tttaacaggc ggtattgtac gcgcaaaaat tcgtccataa ccgcctgttg gatttccagc
     2521 cgctttgaaa cgggggaggc gaagcggacg atgatgcggt atgccttgtc atcgtacggc
     2581 acgcgggtaa cgcgcggtct ggcggcgggc gtgataaaca gtttttccgc ctgcacgttt
     2641 tccaaatgcc gttggatggc ggggatgtag ggcgcgcaca agggctcgag tacggctttc
     2701 agacggcata cggcttcatc cgaatccaaa tggatgggga cggggatttc gaccgtatgg
     2761 atgacgtagt cgcccaaaat attgtcgcgg cgcacgggat ggctcaacaa caggctgttg
     2821 gggaaagaaa cggtggttcc cgcaagctgt ccgaccaggg ggttcggacc gacctgcatc
     2881 atcagcgtgt tcaacaggtt gatgtcgacc acgcgcccgc gcaggccgtt gatttcgata
     2941 tagtcgccga ccgagtattg ctgggtggca gaccttaaaa tactgcccga cagacacata
     3001 atcagttcct ttgtcgccac gacgaccgcc gccgccaccg caaacatcga caaagccagc
     3061 gtttggattt gcgccgacca gataaatgcc agcgaaaaca gcaccaaaag cagcgttata
     3121 ttgcggctgg caaccaaaaa ccgccgcttg ctttcgatgc cgaaatccgg atgccgtttg
     3181 aagtggatat tcaacagaag ggcgcgcgcc agcagcaaag ccgcgaccac cgccacggat
     3241 tcgaccgcct ccgcacgtat cgggacggca ccgagccaag tgtccaacat attccatatt
     3301 tccatttccg cgcccctgtc cgaaaagtat agggtgtaga ttttagtggc aaaaaaacgc
     3361 ttttgccact ttacgggaca atccccgacc tgatgccgga ggcaaacgta ccgtttcatc
     3421 ttttcccggc ggcacactga tgcggataac gctgaattag gcggcaaaat cggctataat
     3481 acacaattat atatgccgtc ccgcccgctt tttctacaag gtgcggactt gttttggcgg
     3541 catcgcaaaa tcttttcaaa atccggcaaa aaatatgact gaacaaaaac acgaagaata
     3601 cggcgccgac agcattcagg tgctcgaagg cttggaagcg gtacgcaaac gccccggtat
     3661 gtacatcggc gacacgcagg acggcagcgg cctgcaccac atggtgttcg aagtattgga
     3721 caacgccatt gacgaagcac tcgccggaca ttgcgacaaa atcaccgtta ccatccacgc
     3781 cgaccattcc gtcagcgtcg ccgacaacgg gcgcggtatg cccaccggca tccacccgaa
     3841 agaaggacgc tccgccgccg aagtcatcat gaccgtattg cacgcgggcg gtaaattcga
     3901 caacaacagc tacaaaatct ccggcggcct gcacggcgtg ggcgtgtccg tcgtcaacgc
     3961 gctgtccgac tgggtaacgc tgaccatcta ccgcgacggc aaagaacact tcgtccgctt
     4021 cgtgcgcggc gaaaccgaag agccgttgaa ggttgtcggc gattccgata aaaaaggcac
     4081 gaccgtgcgc ttcctcgcca gtgcggaaac cttcggcaac gtcgaataca gcttcgacat
     4141 cctcgccaaa cgcatccgcg aactttcgtt cctgaacaac ggcgtggaca tcgaattgac
     4201 cgacgagcgc gacggcaaac acgaaagctt cgccctttcc ggcggcgtgg ctggattcgt
     4261 gcaatacatg aaccgcaaaa aaacgccctt gcacgaaaaa atcttctacg cgttcggcga
     4321 gaaagacggc atgagcgtcg aatgcgcaat gcaatggaac gacagctatc aggaaagcgt
     4381 gcagtgcttc accaacaaca tccctcagcg cgacggcggt acgcacctga ccgcgttgcg
     4441 ccaagtgatg acgcgcacca tcaacagcta catcgaagcc aacgaagtcg ccaaaaaagc
     4501 caaagtggaa accgccggcg acgatatgcg cgaaggtttg acctgcgtgt tgtccgtcaa
     4561 actgcccgac cccaaatttt cgtcccaaac caaagacaaa ctggtttccg gcgaaatcgg
     4621 tcccgtcgtc aacgaagtca tcagccaagc cctgaccgac ttcctcgaag aaaaccctgc
     4681 cgaagccaaa atcatcaccg gcaaaatcgt cgatgccgcc cgcgcgcgcg aagccgcccg
     4741 caaagcccgc gaaatcaccc gccgcaaagg cgtaatggac ggcttggggc tacccggtaa
     4801 actcgccgac tgccaagaaa aagaccccgc attgtcagaa ctttatctgg tcgagggcga
     4861 ttctgcaggc ggttccgcca tgcaggggcg cgaccgcaaa ttccaagcga ttttgccgct
     4921 taagggcaag attttgaacg tcgaaaaagc gcgctttgaa aaaatgctcg ccagtcagga
     4981 agtagccgct ttgattaccg cgttgggcgc gggcatcggc aaagaagaat tcaatgccga
     5041 gaagctgcgt taccaccgca tcattatcat gaccgatgcc gatgtggacg gcgcgcatat
     5101 ccgcaccttg cttttgacct tcttctaccg ccaaatgccc gaactggtcg agcgcggcta
     5161 catctatatc gcccagccgc ctttgtataa agcgaaatac ggcaaacagg aacgttacct
     5221 caaggacgag ttggaaaaag accaatggct gctcggtctt gccttggaaa aagccaaaat
     5281 catttcagac ggccgcacca tcgaaggcgc agaacttgcc gacaccgcca aacaattcct
     5341 gttggcaaaa accgtcatcg aacaggaaag ccgcttcgta gacgaactcg tcctgcgcgc
     5401 catgctacac gcgtcgccca ttgatttgac gtcgtctgaa aacgccgata aagccgttgc
     5461 cgaactttcc ggtctgcttg acgaaaaaga agtcgccctc gaacgcatcg aaggtcatga
     5521 aggacaccgg ttcatcaaaa tcacgcgcaa actgcacggc aacgtcatgg tcagctacat
     5581 cgaacccaag ttcctcaaca gcaaagccta ccaaaccctc acccaaaccg ccgccgcgct
     5641 caaaggcatg gtcggcgagg gtgccaagct ttacaaaggc gaaaacgggt acgacgcgga
     5701 cagctttgaa accgctttgg acatcttgat gagcgttgcc caaaaaggta tgtccatcca
     5761 acgatacaaa ggcttgggcg agatgaaccc cgagcagctg tgggaaacca cgatggatcc
     5821 cgccgtgcgc cgcctgttga aagtgcgcat cgaagatgcc attgccgccg acgaagtgtt
     5881 cgttacgctg atgggcgacg aggtcgagcc gcgccgtgcc tttatcgaaa acaacgcgct
     5941 gattgcccaa aatatcgacg cataagtgcc gttttaaaaa aggagacggg catcgtgccg
     6001 cgtctccttt ttggttggtc aaacggaacc tgtgccgtct gaaaaaccgt cggagcaaaa
     6061 tatgatcagc attttcgata ttttcaaaat cggtatcggg ccttccagtt cgcatacggt
     6121 cggcccgatg aaggcagccg ccgcctttgc ggcaggtttg gatgcacagg ctgttcgcat
     6181 cgtcatcgac atttacggct cgctcgcact gaccggatac ggacacggta catttgacgc
     6241 gctgatgctc ggtttggaag gcagcctgcc gcacgacatc ccgcttgccg gcattcccga
     6301 acgcctcgaa cgcatccgca cgcagcacat cctccggctc aacgggcaag aaatccgctt
     6361 catccccgac cgcgacctga acatactcgg caatcaagtg ctgcccaaac accccaacag
     6421 cctgcgtttt accgcctatg cttcagacgg cacggtattg aatgaacagg tttattattc
     6481 ggtcggcggc ggctttgtcg ttaccgaaga agattttgac cggcaggcgg aaacggaaaa
     6541 agccgttccc tatccctata ccagttgcgc cgaactgctt gcccgatgcc gtctgaaccg
     6601 gctcgacatc tccgaagtcg tgttggcaaa cgaagccgcg cttgccggat gcggcgaagc
     6661 cgaaatccgc cgccgcgttg ccggcgttgc cgatgtgatg gaaggctgca tcaaacgcgg
     6721 tttggcggca gacggcgagc tgcccggcgg attgaacgtc cgccgccgcg ccccgcagct
     6781 tgccgccaag ctcaaagtcc tgcgcgaaac cgaaatcgtc aacacccagc tctggccgat
     6841 ggtgtacgcc atggcggtca acgaagaaaa cgccgccggc ggacgcgtcg ttaccgcact
     6901 gaccaacggc gcggcaggca tcattcccgc cgtattgcac tatttccgca agttcaatcc
     6961 gcacgccaca caggaacgcg tcgaaaactt cctgctcacc gcaggcgcaa tcggcatcct
     7021 ctacaagacc aacgcctcca tttccggtgc ggatgtcggc tgtcagggcg aagtcggcgt
     7081 agcgtgttcg atggcggcgg gcgcatacgc cgaagtcatc ggcggcacgc ccaaacaagt
     7141 ggaaaacgcc gccgaaatgg cgatggaaca ccatttgggg ctgacttgcg accccgtcgg
     7201 cggactggtg caaatcccct gcatcgagcg caacggcatc gccgccgaaa aagccctcaa
     7261 actcggcacg ctcgcgcttt tggaagacgg cacggacaaa aaagtctcgc tcgacgaagt
     7321 catccgaacc atgttacaga cagggcgcga catgaagtcc acctacaaag aaacctcgct
     7381 tgccggactc gccgccacgc tccggaaaaa agccgtcccc gtatccgtgc gcgtggtcga
     7441 gtgctgagga tggacagaaa cgaaaatgcc gtctgaaaac agttttttag acggcatttc
     7501 atttatttct tgcggcttta ccgtttcagt gccagcgtca acacgcccgc cgtaaccagc
     7561 cccaagccta tccattcctg cgtgttcggg cgttcgtcta agaaaaccac cgccatcagg
     7621 aatgcaaacc ggcgttgttt gcagcatccg gcaagaattt ttatagtgga ttaacaaaaa
     7681 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcacttggtg
     7741 cttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt
     7801 ttgttaatcc actataaaag gtcttttcta aaatatgcag atattcgaat atcgaatttg
     7861 ccttatgatt acggttttct gtcttatctg ccttaaaacg ccggtattcc gtttgaacca
     7921 aattatattt gccgaaacac tcaaaaatct cccttacctg cccgacagac atcaagcctt
     7981 cgttgttgta gctgagaaaa atatattgaa agtccgcatc cctaatcaaa gcctcaaata
     8041 ccggaaccac atccgatttt gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc
     8101 ggatgggaat ggcaaaatcc ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac
     8161 caaccggagg ttggtattcc ttaact
//
LOCUS       DAWHXS010000084         8098 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWHXS010000084 DAWHXS010000000
VERSION     DAWHXS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.084"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..573
                     /locus_tag="WIE22_001559"
     CDS             <1..573
                     /locus_tag="WIE22_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ4127623.1"
                     /translation="DLGKLSPEAQLAAASLLQDSAFAVKDGINSARQWADAHPNITAT
                     AQTALAVAEAAGTVWRGKKVELNPTKWDWVKNNGYKTPAARPMQTLDGEMAGGNKPVV
                     KSIRPTTRDELRQALQEQGFRRTGSDEAQYETWKGPDGVKIDIRPNGEVIRTQRVPRT
                     DGVQGKYPQRQNYEGNPLPNNRHHSGYFIK"
     gene            570..887
                     /locus_tag="WIE22_001560"
     CDS             570..887
                     /locus_tag="WIE22_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127624.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            957..1697
                     /locus_tag="WIE22_001561"
                     /pseudo
     CDS             957..1697
                     /locus_tag="WIE22_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1701..2102
                     /locus_tag="WIE22_001562"
     CDS             1701..2102
                     /locus_tag="WIE22_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127625.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            2261..2603
                     /locus_tag="WIE22_001563"
                     /pseudo
     CDS             2261..2603
                     /locus_tag="WIE22_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2743..3099
                     /locus_tag="WIE22_001564"
     CDS             2743..3099
                     /locus_tag="WIE22_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ4127626.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNKKTVIFHF"
     gene            3272..3646
                     /locus_tag="WIE22_001565"
                     /pseudo
     CDS             3272..3646
                     /locus_tag="WIE22_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3684..3946)
                     /locus_tag="WIE22_001566"
                     /pseudo
     CDS             complement(3684..3946)
                     /locus_tag="WIE22_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4602..5588)
                     /locus_tag="WIE22_001567"
     CDS             complement(4602..5588)
                     /locus_tag="WIE22_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ4127627.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            complement(5628..6428)
                     /locus_tag="WIE22_001568"
     CDS             complement(5628..6428)
                     /locus_tag="WIE22_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ4127628.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLNTYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            6553..7896
                     /gene="argG"
                     /locus_tag="WIE22_001569"
     CDS             6553..7896
                     /gene="argG"
                     /locus_tag="WIE22_001569"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ4127629.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            7971..>8098
                     /locus_tag="WIE22_001570"
     CDS             7971..>8098
                     /locus_tag="WIE22_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ4127630.1"
                     /translation="MKEYKVVIYQESLLSSLFFGATKVNPVNFSAFLNKQTPEGWRV"
BASE COUNT         2330 a         1759 c         1842 g         2167 t
ORIGIN      
        1 gatttaggaa aattaagtcc ggaagcacaa cttgctgccg cgagcctatt acaggacagt
       61 gcttttgcgg taaaagacgg catcaattcc gccagacaat gggctgatgc ccatccgaac
      121 attacagcta ctgcccaaac tgcccttgcc gtagcagagg ccgcaggtac ggtttggaga
      181 ggtaaaaaag tagaacttaa cccgactaaa tgggattggg ttaaaaataa cggctataaa
      241 acacctgctg cccgccctat gcagacgttg gacggtgaga tggcaggagg aaacaagcca
      301 gttgttaaat ctatcagacc aactacgcga gatgaattac gtcaagcatt gcaagaacaa
      361 ggttttagac gtactggttc agatgaggct caatatgaaa catggaaagg tcctgatggc
      421 gtgaaaatag atattcgtcc aaatggagag gttataagaa cccaaagagt gccgcgaacc
      481 gatggtgtac agggaaaata tccgcaacga caaaattatg aaggcaatcc attgccaaat
      541 aatcgtcatc attctggata ttttatcaaa tgaaaaaaaa tatttttcac aatgtaagcc
      601 tttatgaaat aatcttttcc gataatggaa atacccttac attatctttt acagatacaa
      661 ttgaaggtaa ttatttcgga tatatcaaat gcagtaatat tttgaatttt aaattagata
      721 caaataattt cgtagattat gaggataagg aagatagctt gtttcccttg tttatacccg
      781 aaatagagct atataaatac caattttata gtgaaattat tattgatgta gggattatta
      841 taaaaatatc tgctgaaaca attaattttg agccactggg aaaatagtaa ctgctttccc
      901 agcagccgta gcaactgtat ttttacccga cggggtaaaa atacagttgc tacatctctg
      961 cgcagcagac tctacaaggt attaataatt taggaaaatt aagcccggaa gcacaacttg
     1021 ctgccgcgag catattacag gacagtgctt ttgcggtaaa agacggcatc aattccgcca
     1081 gacaatgggc tgatgcccat ccgaatataa cagcaacagc ccaaactgcc cttgccgtag
     1141 cagaggccgc aggtacggtt tggagaggta aaaaagtaga acttaacccg accaaatggg
     1201 attgggttaa aaataccggc tataaaaaac ctgctgttcg ccatatgcag actaaggcgt
     1261 taggtacggt agatgaaatt ggcgatacag tacagcaggt tgggaaacag gctagcggac
     1321 aaaaaaccag cggtggtaat cctgcgattg atagcgaccc ctatagcccg agtagtgtgg
     1381 cagctcgcat agaaaccggt aaggtgcgca gtgatttaca aatcaaagac attttgagca
     1441 atactactca aaggagtaaa acaaaaggtc ccgctgttca gtatgataaa gtgggggatt
     1501 acaatgacgc actaaatgat tttaatagtc tgaatgttcg aaatgtacaa acacgtccta
     1561 atggaacgat aacgggcaat ttacctgata ggcgtgcggt taatgctcgt aatgatagta
     1621 gtggtggaga accaacactt gaaataacaa ttagtaataa ccgaaaaata aaaatcagat
     1681 atggaaatac acgataaatt atgaaattaa aaagcttaga tttcccaact ggctatttct
     1741 attttgataa tgcagcaata aactctgata aagtagaagt tatagcagtt ggttatagaa
     1801 atacggataa aaccataaaa atttttattg aagatgttat tcattttagg gttgttgatg
     1861 aatcgtattt tatagatact tttatggatt taatttcgga agatgcagat agagctttgc
     1921 ttcatgaaaa tggtggtcaa tctttttttg aacttcttga tgagtgttat gcggaatgga
     1981 tattgaaaga aagttatttt cctttgaata gagaattctt taaatactat atttttatgt
     2041 ttgagcaaac attcatagaa ataattggtt ctagtgcaac gtattcaatt attgagggct
     2101 agcgtaagat gagtaataag ttgcctatct ttctttcagg cagcctgaaa ataaaactac
     2161 ccaagttgat ggtgtacctg tatcagtgaa gggaaatttt gttgatggta aatttcgcat
     2221 tggtacggca acaatgaaat cattttaaat tgagctagaa atgaacctag aaaattatga
     2281 aaacatttta ataaaattac ttttttatca taacaactta gtaaatgaat attcttattt
     2341 tattgaaaat aaaaatttat ttaaaatagt atctcgaact aaaacgagta agggcttttt
     2401 ttcactatag aaaaaccatt aaattttcta agcaaaaaaa cttattttga gtttaatttt
     2461 aaatatttac actcagggaa agaacgcttt ggttcgttta tgtgctggat aaatactaat
     2521 ttaatggaat ttgagggggt ttttttaacg acctgctccc tgataatatg ataataaata
     2581 acttttttga aataaatgat taacgataca ccaataaaaa ttggtggggt aaccgtatgg
     2641 atgatattcg cggcatcatc caaggtgcgg ttaatccttt aatttacaag gtaagcaagc
     2701 aaaagattat ttacaacaaa ctcatatcag gaacttagac aaatgaatga acacaacctg
     2761 ttaattttct gtttaaaaga caatgtttca attagtgaat atactgaaat gattgattgg
     2821 gcttataaaa atattcaatc tgaaacagtt gtagaaatta cggaaagtca aattattgaa
     2881 tatcaaaatc gtggattatg gagacttgtt tctgaaatta ccgataattg gttatttgga
     2941 ccaagtgagg gggattggct aatagataag gaaagtattt tggctgtaaa agaaaaatta
     3001 caaaattcag atttttctac agagccctta gtgaaaaata ttattcatgt acttgaatat
     3061 gctataaaaa ataaaaaaac agtaattttt catttttgag actaatccaa tttttagtaa
     3121 tattgatgca gagcaagcag cattagatgc cgcaaacatg gggagaagct attcaattta
     3181 gaattaaaaa acaaattgaa aatgaactag caccaccaaa ttggtctacc cagtttccta
     3241 atggtagtat ttatgatcct aaggtaacga aatgattatt caaaatgaat ttaatttata
     3301 tcctagtaat atgcttcctg aaaggttttg ttatcctgaa aagtatgttc gtatctctaa
     3361 cgatacatct ttaatacctt atattcagcc acataatttt cactggtagt ttgagaatta
     3421 tggaacagaa ggggcagaag tagcttatat atttagaaat tctatcctgc ctgatttaaa
     3481 tcttatccca ttcgctagta atggagaatg ggaagcttat tttgatggta atgatgtaac
     3541 aggaaattct agggttattg tcattaattt agataatata gaaaaccatg aattttttaa
     3601 tagttttgaa gattggcttg aattagcaat taaggatact tggtaagcag ctatctataa
     3661 agagatgagg ctgccttgga caactaggat aaactcgatt ttactaattg ttttaaaatg
     3721 gaacaagaac ttttatttca ctgttgttaa aacgccattc gcactccttt aaatacagct
     3781 caaaatgcgc tttgggaatg ccgttaaact tgcgtaaatg acgttttgct tgattccaaa
     3841 agttctcagt tccattaata tggttttgtc gttcggcaaa atgtgtgctg tgattgatac
     3901 ggaaatggct aaattcgccc gcatccaata catcatagcc acgataacaa aatgagttta
     3961 ttttgtttat accgtcttag acgactttct ctcataggga taattctaac ttaatttgaa
     4021 tttccctagt gatctagggc agcccctaaa ttaataaagc agcacaactc cttttgccga
     4081 tgttccggac tgtcaaacga ctgttcctca tgccacatct ccatcaaggt acggataacc
     4141 cgctccgcct taccgttggt ctgcggacaa gcaaatcggg caagcctcca accaatccca
     4201 ttattataac aagctgcacc gaaagcatgt tggacggctc tttatattac ctatcattgt
     4261 cagagtaaac gtactcaatc aggtacaagc aggggtcagc cagatgttcg gtcagaaact
     4321 tggcagcact gtctgcggtt ttgtccggca aaatggcaga gtataaaaat cgtcaatagc
     4381 gacaaacagg taatctcgtt tatcggcggc cttctgtcct ttgagcaaca acaaccgatc
     4441 ggtatcagga tgaacaaaac ctcccgggac aacctgcctt ttacggcttt aagtgcacgg
     4501 taaatagtga cgcggctgac ttagtggcag catactgggg aggtgagtgt ttttgtgtat
     4561 atttttattt tggtattccc ttagaaatac tgtaaacaac gctaccggac ggcctgcagg
     4621 gcttcgcgca cgcttgcttt gagttctgcg ccgaagcgtc tgcccaagat tctgccgaaa
     4681 tcgtccttcg gagtgtaatc caccacatcg ggggctttga ccacgtctcg cgccacgctg
     4741 taaatattgc cgagtccgtc caccagcccg actttcagcg catccgcgcc tgtgtacacg
     4801 cgaccgctga acacgtcggg atattgtcgg aatttgaggc ggccgccgcg tccggttttg
     4861 acggctttga tgaactcacc gtgtatgccg gtcagcattt cttcccagat ttttgactgt
     4921 tcgggcgttt cgggcgaaaa cggatcgccc atgcctttgt tgctgcctgc aattttaacc
     4981 ctgcgtttca cgccgatttt ttccatcagg ccggtcgcgt cgaaactgct gccgataacg
     5041 ccgatgctgc cgacgatgct ggacgggtcg gcatagattt tgtccgccgc cgccgcgatg
     5101 tagtagcagc cggacgcgca catatcttcc gccacgagat aaacgggaat gccggggtgc
     5161 tgcgccttca gacggcgtat ttcttcaaaa gcggtgttgg agacgacggg cgaaccgccg
     5221 gggctgttgg cgcggatgac gatggctttt gcctgcgggt ttttgtaggc ggcctccata
     5281 ccgtctttga gttttttgac ctggtcttct acaccgttgc cgatttcgcc gtacaggttg
     5341 acgactgcgg tatgcggcgt gttgcccgcc aactgcaatg cggcttcgtc ttttcggaaa
     5401 atgcctgcaa tcagggcaac caaaatcagg gtgctgacgg cgcgccagat gtttttccac
     5461 atccgctccc tgcgcctgtc ctgataggcg gacagcagca cttcgcgcat gatgtcgcgc
     5521 tcccataagg tttcccccgc attttttgct tcgggtgctt cgttttctct tctgattcgg
     5581 tattgcatgg ttttccttaa atattgtccg atttgggcaa acggttttca gtttacccga
     5641 tttttcagct ctgctcccaa tccgtccaag ctgtgcaaca cttccgccca cgccgcgtcc
     5701 aaaaggttga cggcttctcc ttcggctttg atgccgaact caatgtgcgg tttgacctgc
     5761 gtgccgtctg aatgcgtcca accgacgctg ggcaggctgt acgaacgcac accgggataa
     5821 gtttgctcga tatgctccat aagcggcgta atgcgcgatt cgggctgctc aaacacatac
     5881 acgctgcggc tgccgcgttc ggtttggttg aagcggtcgg cgtaataagt attcaaaacc
     5941 cattccgcca tcgggtgcgc catcacagga aagccgggga agaaataatg ctcgcggatg
     6001 gaaaatccgg cgatgttgtt gaacgggttg ggcacaagct ctgcgccctc ggggaaatcc
     6061 gccattttca ggcgttgggc gtgttccggc gaatcaagcg gctcgccgcg tttctgggtt
     6121 atgccttcga taaacttggc ggcttcagga tggcggacga caggcaaatc caaagcagca
     6181 gccgcggctt ggcgcgtgtg gtcgtcgggc gtggcgccga tgccgccggt aacaaacgtc
     6241 ggtatgccgt ctgaaaagct gcggcgcagt tgcctgacca gtaaatcggg ttcgtcgggt
     6301 agatattgca cctgattgag cttcagccct ttggattcga gcagggattt gaaaaaggcg
     6361 aaatgcttgt cttggcggct gccgtgcagg atttcgtcgc cgatgatgat gaggttgaac
     6421 gcgttcataa tgttttcggt tttaaatacc gcctgaaacg gcggaatacc cgttcagacg
     6481 gcatgatggc tgacaatatt tcctatttat aagataatgg ctttgatttt tcaagctacc
     6541 gaggattttt tcatgaacca aaaccatact attttacaaa atctccccgt cggtcagaaa
     6601 gtcggcatcg ccttctccgg cggacttgat acctccgccg cgctgttgtg gatgaaactc
     6661 aaaggcgcgc tgccttatgc ctacactgcc aacctcggcc agcccgacga agacgattac
     6721 aacgccattc ccaaaaaagc gatggaatac ggcgcggaaa acgcccgctt aatcgactgc
     6781 cgcgcgcagt tggcacatga aggcatcgcc gccatccaat gcggcgcgtt ccacgtttcc
     6841 accggcggca tcgcctattt caacaccacg cctctgggcc gcgccgtgac cggcaccatg
     6901 ctcgtttccg caatgaaaga agacgatgtg aatatttggg gcgacggcag cacctacaaa
     6961 ggcaacgaca tcgagcgttt ctaccgctac ggtttgctca ccaatcccgc gctgaaaatc
     7021 tacaaaccct ggctcgatca gcaatttatc gacgaactcg gcggccgtca cgaaatgagc
     7081 gaatttctga ttgccaacgg cttcaactac aaaatgtcgg tggaaaaagc ctactccacc
     7141 gattccaata tgttgggtgc cacccacgaa gccaaagact tggaattttt gaactcgggc
     7201 atcaaaatcg tcaaacccat tatgggcgtt gccttttggg acgaaaacgt cgaaatcgaa
     7261 cccgaagaag tcagcgtacg ctttgaagaa ggcgtgccgg ttgcactgaa cggcaaagaa
     7321 tacgctgatc ccgtcgaact cttcctcgaa gccaaccgca tcggcggccg ccacggtctg
     7381 ggtatgagcg accaaatcga aaaccgcatc atcgaagcca aatcgcgcgg catctacgaa
     7441 gccccgggaa tggcgttgtt ccacatcgcc tacgaacgct tggtaaccgg catccacaac
     7501 gaggacacca tcgaacaata ccgcatcaac ggcctgcgcc tcggacgttt gctctaccaa
     7561 ggccgctggt tcgacagcca agccctgatg ttgcgcgaaa ccgcccaacg ctgggtcgct
     7621 aaagccgtta ccggcgaagt taccctcgaa ctgcggcgcg gcaacgacta ctcgattctg
     7681 aacaccgaat cgcccaatct gacctaccaa cccgaacgcc tgagtatgga aaaagtcgaa
     7741 ggtgcggcgt ttaccccgct agaccgcatc ggacagctca cgatgcgcaa ccttgacatc
     7801 accgacaccc gcgccaaact gggtatctac tcgcaaagcg gtttgctctc gctgggcgaa
     7861 ggctcggtat tgccgcagtt gggcaataag aaataaggtt tgctgttttg catcattagc
     7921 aacttaaggg gtcgtctgaa aagatgatcc cttatgttaa aaggaatcct atgaaagaat
     7981 acaaagtcgt catttatcag gaaagcctgt tgtccagcct gtttttcggc gcgacaaagg
     8041 tcaaccccgt caatttcagc gcgttcctca acaaacaaac ccccgaaggc tggcgggt
//
LOCUS       DAWHXS010000085         7972 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWHXS010000085 DAWHXS010000000
VERSION     DAWHXS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.085"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(113..691)
                     /gene="grpE"
                     /locus_tag="WIE22_001571"
     CDS             complement(113..691)
                     /gene="grpE"
                     /locus_tag="WIE22_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ4127631.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            875..1693
                     /gene="cysE"
                     /locus_tag="WIE22_001572"
     CDS             875..1693
                     /gene="cysE"
                     /locus_tag="WIE22_001572"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ4127632.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            complement(2050..3561)
                     /gene="ubiB"
                     /locus_tag="WIE22_001573"
     CDS             complement(2050..3561)
                     /gene="ubiB"
                     /locus_tag="WIE22_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ4127633.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(3596..4060)
                     /locus_tag="WIE22_001574"
     CDS             complement(3596..4060)
                     /locus_tag="WIE22_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ4127634.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            complement(4057..4203)
                     /locus_tag="WIE22_001575"
     CDS             complement(4057..4203)
                     /locus_tag="WIE22_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127635.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(4220..4558)
                     /gene="erpA"
                     /locus_tag="WIE22_001576"
     CDS             complement(4220..4558)
                     /gene="erpA"
                     /locus_tag="WIE22_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ4127636.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(4690..5376)
                     /locus_tag="WIE22_001577"
     CDS             complement(4690..5376)
                     /locus_tag="WIE22_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4127637.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            5564..5836
                     /locus_tag="WIE22_001578"
     CDS             5564..5836
                     /locus_tag="WIE22_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127638.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            6044..7972
                     /gene="dnaK"
                     /locus_tag="WIE22_001579"
     CDS             6044..7972
                     /gene="dnaK"
                     /locus_tag="WIE22_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ4127639.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         2184 a         2204 c         1781 g         1803 t
ORIGIN      
        1 aatgccgtct gaagcccgac aggcgggttc agacggcatt gccgctttac ttcgatcggt
       61 ttatacgcaa aacgcgcttc aggaaataaa tcagattatt ccccagacgc cttcaggctt
      121 ccttctgcgc caccgtaacc atagccgggc gcaacacgcg gtcggacagc gtataaccct
      181 tcttcatcac acccaccacg gtattcggct cctgttcgct tgccaccgcc tgcatcgcct
      241 gatggatatt cggatcgagc ttatcgcccg ctttagggtt gatttccttg atttgcgtag
      301 catcaaatgc tttctgcaac tcgttcaaag tcatctgcac gcccattttc agcgcatcga
      361 aattgccgct ctgatccaaa agcgccattt ccagataatc cttgaccggc agcatttcca
      421 cggcaaactt ctgtccggcg aacttgtgcg tatccgcaat ttcctgctgg tggcggcggc
      481 gcaggttttg ctcgtttgcc aaagcgcgca gctgctcgtc tttcaactgc gcttccagct
      541 cggcaatccg cgcctgcaaa tcctcataag ccggctctgc ggcagcctgt tcctgcacac
      601 cgtccgcatt tcctactgtc tcgacggttt ccaccgcctc cacattttca accgcttctt
      661 cactgttttg ctgctgtgtc tgttcgctca tatcgtatcc cttaaaacaa attggaaatc
      721 aaaatccagt tattacccgc gcaagataag gacattttca agaaacttca agcaaaagca
      781 gagaatttca aattcatttt caaatcccct accgaaaaaa taatatagac ggtaagatac
      841 tgccgaccat atcgattttc aaacgtcatc actcatgaaa aaagaccatt taaacacaac
      901 cggtttcgac ctctggcaca ccatccgcga agaaaccgcg gccgcggtct ccgccgaacc
      961 gatgctggca agttttttgc accaaaccgt gttgcgccac gagtccctcg gctccgtcct
     1021 tgcctaccac ctttccagca aactcggcag cccgattatg gacgtgcgcg cgctgtttga
     1081 aatctaccag caggcgttgg gcagcgacac ccaaatcggc aaatgcgtcg aagcagactt
     1141 aaaagccatc tacgaacgcg atcccgcctg cgacgaatat tcgctgccgc ttttatattt
     1201 caaaggcttc cacgcgattc aggcacaccg catcaaccat cggctgtatc tcgacgggcg
     1261 caaaacgctg gcgtatttcc tgcaaaaccg tatgtccgaa gtattcggcg tggacatcca
     1321 tcccgccgcc cgtttcggat acgggctgat gctcgaccac gccaccggct ttgttgccgg
     1381 cgaaaccgcc gtgttgggca acaatatttc gattttgcac ggcgtaacgc tcggcggttc
     1441 gggcaaagaa ggcggcgacc gccaccctaa aatcggcgac ggcgtgatga tcggcgcaaa
     1501 cgcctcgata ttgggcaata tccgcatcgg cagcaatgcc aaaatcgggg cgggcagcgt
     1561 cgtggtttca gacgtgccgc cgtccatcac ggttgtcggc gtacccgcca aacccgtggc
     1621 gcgatcgctc aaaacaccgt cggcggatat ggatcaaaat atccagttta ccgaaatcga
     1681 ctttatgatt tgaatatccg caccaatgcc gtctgaaacg ccaaacgggc ttcagacgac
     1741 attgcccgct tcaagcctgc gccttgatat gccgccgatg tcggcacttg ccagtcggac
     1801 aaaatgcctt ttattcaata cacccgattc ccacgcaacc ggaaatatag tggattaaca
     1861 aaaatcagga caaggcgacg aagccgcaga cagtacggat agtacggaat cgattcactt
     1921 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgagacaa cgccgtactg
     1981 gtttttgtta atccactata accgatttcc ttataaacgg gcggagtcgg gcgatgattc
     2041 ggacaaagcc tatttcaaaa gcaaaatcag cagcaaaaca accgcaatca cagccagcca
     2101 caggctttgc cgctgctgca ctttgaccaa atggatatag gcatcacgca tttcctgctg
     2161 gcggttttca tcaatcaacg cactgatttt gcgcggcaat gaagggatga tttgcgccca
     2221 gtcgggggct tcgtttttga ggttgcgcca aagggcttta gggccgacct gcccgttcat
     2281 ccatttcacc aaaaacggtt tggcggtttt ccacaagtcc aaatcgggat cgagctggcg
     2341 gcccaagcct tcgatgttga gcagcgtttt ttgcagcagc acaagctgcg gttggatttc
     2401 gacattgaag cggcggctga cttcaaacag gcgcatcagc accaagccga aggaaatctg
     2461 cgaaatcggt ttgttgaaca ccggttcgca cacggcgcgg acggcggctt ccaattcttc
     2521 cgcacgcgtg tcggcaggca cccagcccga ttcgatgtgg gcggtggcga cgcgccggta
     2581 atcgcggttg aaaaaggcga ggaaattgat ggcgagataa cgtttgtcgt aatcggtcag
     2641 cgtgccgacg atgccgaaat cgagggcgat gtagcggttg tcggcggcaa ccaaaatatt
     2701 gccggggtgc atatccgcgt ggaaaaagcc gtcgcggaag acctgcgtga aaaagatttc
     2761 cacgccgtaa tcggcgagtt tgtgcaaatc gatgccgtct gctttgagtt tggcgatgtc
     2821 ggcaaccggc gtgccgtcca tccattcgat tgtcagcacg tcgctggtgc agtagtcgta
     2881 aaacaccttg ggcacaatca gcatattgct gttttggaaa ttgcgcccga gctgtccggc
     2941 attggcggct tcgcgcatca aatccaactc gtcgtgcagg tatttgtcga actccgccac
     3001 cacttcgagc ggcttcagac gcctgccgtc tgaaaacaag cgttcgaccc aggccgcgcc
     3061 gaaacgcatc agcgacaaat cctgttcgat aaggggcaaa aggttggggc gcaaaacttt
     3121 aaccgccact cgttcgcccg aatgcaggcg ggctttgtgt acttgggcga tggacgcgct
     3181 ggcgatgggc tcggtttcaa attccgcata cagcttttcg atggactgac ccaacgattt
     3241 ttcgatttgt tcacgcgaaa gccgcgcgtc aaaaggcggc actttgtctt gcagctttgc
     3301 cagttcgacg gcgtaatcgt gcggaatcaa atcggggcgt gtggacaaaa cctgcccgaa
     3361 cttgatgaaa atcggcccca ggctttccaa ggcaaggcgc agacggacag caggcggttc
     3421 gtttttcaat ttggacgact gcggcatcat tttcagcaga gcgcatatcc aaccgctacc
     3481 catcagcgaa gcacacagac ctgccagccg atagcggtaa aaagtcccga caataaccgt
     3541 caggcgtttc aaccatttca tatataaaat cccgaataca gcatttatcg gacaatcaaa
     3601 aaatcttacc gagcagatag cccaaaaagg caatcagcag cccgaaaacc agcagtttga
     3661 tattgcggga cggcggttcg ggttgcgaag gtgcggaaat atcatcttcc cgcccgccgt
     3721 gaagttcaag atattcgggc atttccccat ccgcgccttc tccttcgccc tccccttcct
     3781 ccggcggcac gaccgtttcc aactccatat atctccgggt aataatatcc aaacccaaat
     3841 agctgaaata atcatgcaac tcttcccgtt tcgcccaagt gcatttttcc tcaaccagca
     3901 cttgaggttt ggaatcgagc gattcgatca atccttccgc cttttcctcg ccgagattct
     3961 ccttaaggca ctcccggata agcccgcgat ccacttgggg cgggtaagaa acatatacat
     4021 cgtacaaatc gtttcggtaa tctttactca tctcaatcat aagggaacaa atacgcctga
     4081 taccgttccg cccgttcctt tcgggaaacc cgattcatct tcaaacaatc ggattatatg
     4141 tgaattcatc ccgataatat attttctaaa agtaaaaatg ccgtctgaac gttcagacgg
     4201 catcacacaa accaagcggt catacggaaa acgacgatcc gcaaccgcag gttgtttccg
     4261 cattcggatt gcggatgacg aattgcgaac cctgcaaact ttccgtatag tcgatttccg
     4321 caccgaccag atattgatag ctcatcggat cgaccaaaaa gaccaaaccg tttttctcaa
     4381 tttcaaaatc gtcgtcgttt ttgatttcgt caaaagtaaa tccgtactgg aaacccgaac
     4441 agccgccgcc attgacaaaa acccgcaatt tcaaatcggg attgttttct tcggcaatca
     4501 aatcggcaac tttggcacag cagctgtcag taaaaataat agggctttca tccgacatgg
     4561 tgttattcct taatataata ttttctgcat tctctctcaa acccccttca aaggcaagca
     4621 ggcttcgcct ttgaaaaagg aattgttcca atagataagt ttgaccgcct gcaatttcaa
     4681 gccccaaact caaatcgtcc tgccgaacca ctcgacacgc ccgataatct ccacatcatc
     4741 ggtcaaatcg ttcaaattga tttcaaaagc aggataagcc tcgtttgcag aaatcacatt
     4801 gataatcccg cccggtacaa tctgtaaacg tttaaccagc agattttcat taatccgcaa
     4861 cacatacaga ccgtccctcg gcgtattttc accatgattg accaaaatcg aatcgccgtc
     4921 attcaaaacc ccctccatcg aatccccctt gacggaaatt acagacaggt ttttcgtatc
     4981 gcgggtaaca taattctcaa tccaatgccg tctgaacgcc attgtaaata ccggttcctc
     5041 atgatcgaca aactgcccgt atcccgcagc cgcccgaata tcatatctcg gcacgaagac
     5101 aaactcgtcc gtatcgactt cattgcccaa agtatcgtaa gcaagggatt tttttggggc
     5161 ttcatccgga aacggattac cctccccggt cagcagccaa tcgatactac accccttcaa
     5221 ctgcttgatt tttttcaatg tttcagactt tggcagaccg ccttcattcc atatcctgct
     5281 gaagcccgca atcgtcattt caatatcgga tgcgattttt gcctgcctcg cttcgctttt
     5341 ccataaaaaa accagtctgt ctttaaaggt ttccataaat atccttccac tccccttcat
     5401 tcaatcatct aaagtaaaca tgaaatatat tttaccttag ctatccaaaa aacggaacaa
     5461 ttatgataaa ctaaatttaa attatgattt ttcttactta atacataatt tttcagattt
     5521 ttccataggt ttaatcctga attgacagaa aaaggataga aaaatgaaga aaaccggcaa
     5581 atatcttatc tatactgcgg catttaccgc attctgcttt gccttccaag aaaaccgttc
     5641 tgaagccaaa cagcccgaca tcactttatc cgcatccctg tgcgaacaat tcaacatact
     5701 gaacgccaaa gatatggatg cagaacaagt ctccctttcc aaagaatgcg acatcatcga
     5761 gtcttcacac gactgggaaa aagagtacgg caacttgaac gaacaggaaa tgctcgccgg
     5821 catcgtctac gaataaacct gcctgccatt tgaaatatta tgcttgaatg cattggagcc
     5881 aaatgtatta aatcaaatat aaaaccaata tattcataaa gttatatact tatagccatg
     5941 ctgtagcttg aaacagcccg ccgcccgccc tatttacagc ccatcgggac aaaatgttcc
     6001 aacatatttc tcaaaataag caaaatcaaa taggattatc cacatggcaa aagtaatcgg
     6061 tatcgactta ggtacaacca actcttgttt ggccatttcc gaaaacggtc aaaccaaagt
     6121 tatcgaaaac gcagaaggcg cacgcaccac gccgtccgtt atcgcttatt tggacggcgg
     6181 cgaaatcctc gtcggcgcgc ctgccaaacg ccaagcggta accaacgcca aaaacactat
     6241 ttacgccgcc aaacgtttga tcggccacaa atttgaagac aaagaagtcc aacgcgacat
     6301 cgaatctatg cctttcgaaa tcatcaaagc caacaacggc gacgcatggg taaaagcaca
     6361 aggcaaagag ctgtctcctc ctcaaatttc cgcagaagtc ctgcgtaaaa tgaaagaagc
     6421 cgccgaagct tacttgggcg aaaaagtaac cgaagccgtg attaccgttc cggcctactt
     6481 caacgacagc caacgtcaag ccaccaaaga cgcaggccgt atcgccggtt tggacgtaaa
     6541 acgcatcatc aacgagccga ccgcagccgc tttggcattc ggtatggaca aaggcgacaa
     6601 caaagaccgc aaagtagccg tatatgactt gggcggcggt actttcgata tttccatcat
     6661 cgaaatcgcc aacctcgacg gcgacaaaca atttgaagta ttggctacca acggcgatac
     6721 tttcttgggc ggtgaagact tcgaccaacg cttgatcgac tacatcattg acgagtttaa
     6781 aaaagaacaa ggcattgatt tgaaacaaga cgtcatggct ctgcaacgtc tgaaagaagc
     6841 tgccgaaaaa gccaaaatcg aattgtccag cggccagcaa accgaaatca acctgccgta
     6901 catcaccatg gacgcaaccg gcccgaaaca cttggcgatg aaaattaccc gcgccaaatt
     6961 cgaaagcctg gttgaagacc tgattgcccg ctctatcgag ccttgccgca ccgcattgaa
     7021 agatgccggt ctgagcactg gcgacatcga cgacgtgatt ttggtcggtg gtcaaagccg
     7081 tatgccgaaa gtacaagaag ccgttaaaga cttcttcggc aaagagccgc gcaaagacgt
     7141 gaaccctgac gaagctgtcg ccgtaggcgc agcgattcaa ggcgaagtgt taagcggcgg
     7201 ccgcagcgac gtattgctgc tggacgtaac ccctctgtcc ttgggtatcg aaaccatggg
     7261 cggcgtgatg accaagctga ttcaaaagaa caccaccatc ccgaccaaag cgtcgcaagt
     7321 gttctctacc gccgaagaca accaaagcgc agtaaccatc cacgtactgc aaggcgaacg
     7381 cgaacgcgct tctgccaaca aatctttggg tcagttcaac ttgggcgaca tcgcacccgc
     7441 accgcgcggt atgccgcaaa tcgaagtcac cttcgatatt gacgccaacg gtatcctgca
     7501 cgtttccgcc aaagacaaag gcactggcaa agcagccaac atcaccatcc aaggttcttc
     7561 aggtttgagc gaagaagaaa tcgaacgcat ggtgaaagat gccgaagcca atgccgagga
     7621 agataaaaaa ctgactgaat tggtcgcttc ccgcaaccaa gccgaagccc tgattcactc
     7681 tgtgaaaaaa tctttggccg actacggcga caaactcgac gcagccgaga aagaaaaaat
     7741 cgaagccgcg ctgaaagaag ccgaagaagc agttaaaggc gacgacaaag ccgctatcga
     7801 tgccaaaacc gaggcgctgg gcgcagccag ccaaaaactg ggggaaatgg tttacgctca
     7861 agcacaagct gaagcccaag caggcgaaag cgaacaagcc aatgcttctg caaagaaaga
     7921 cgatgatgtc gtagatgccg actttgaaga agtaaaagac gacaaaaaat aa
//
LOCUS       DAWHXS010000086         7946 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWHXS010000086 DAWHXS010000000
VERSION     DAWHXS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.086"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(184..1980)
                     /locus_tag="WIE22_001580"
     CDS             complement(184..1980)
                     /locus_tag="WIE22_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ4127640.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGTNPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2438..3691
                     /locus_tag="WIE22_001581"
     CDS             2438..3691
                     /locus_tag="WIE22_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ4127641.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3846..5357
                     /gene="lysS"
                     /locus_tag="WIE22_001582"
     CDS             3846..5357
                     /gene="lysS"
                     /locus_tag="WIE22_001582"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ4127642.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHTQYGEIGKEELDPQGIPVKIAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEILDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANGESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5434..5622)
                     /locus_tag="WIE22_001583"
     CDS             complement(5434..5622)
                     /locus_tag="WIE22_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ4127643.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLTALSEWVGQLESE"
     gene            complement(5626..6504)
                     /locus_tag="WIE22_001584"
     CDS             complement(5626..6504)
                     /locus_tag="WIE22_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ4127644.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLVVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
     gene            complement(6534..7661)
                     /locus_tag="WIE22_001585"
     CDS             complement(6534..7661)
                     /locus_tag="WIE22_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ4127645.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1726 a         2010 c         2218 g         1992 t
ORIGIN      
        1 aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggcttcgtcg
       61 ccttgtcctg atttttgtta atccactata tttgcttgcg gcaagatgaa aacggtgcgg
      121 gatgttttgg gaaaccaatg ccgtctgaag ggctttcaga cggcattttt tgcgccgtgc
      181 tgtttaacgc gccagcggtt cggtgcgttt gatcagccac gcttttgccg cgccttcggt
      241 cagcggctcg aggcggcggc ggacttcggc gtggtagcgg ttgacccagt cgatttcgcc
      301 gtcggtcatg agggcggtgt ccatcaggcg ggtgtcgatg gggcagaggg tcagggtttc
      361 aaaacagagg aagctgccga attcggtttc ttgaggggcg gcgacggctt ggttggcggc
      421 aaggttttca atgcggatgc cccattttcc cgggcggtag agtccgggtt cgatggaggt
      481 aaccatgcct tttttcatgg cggtttcggg cgtggcgggg gcggcgaagg cgatgcgctg
      541 cgggccttcg tggacgttga ggaaatagcc tacgccgtgt ccggtgccgt ggccgtagtc
      601 gcattgcgcc tgccacaggg gtttgcggca aatcgcatca atcagcggcg aggggatgtt
      661 ttctgggaac acggcttcgg caagcgcgat atgggctttg agaacgaggg tgttgtcgcg
      721 tttttgttcg gcggtgggtg tgccgacagg gacgacgcgg gtgatgtcgg tcgtgccgcc
      781 tttgtattgc gcgccggagt cgatgagcag cagcccgttg ccgctgatgg tgctgtggct
      841 ttcgggcgtt gcgctgtaat gcggcagggc gccgttggcg ttgaagcctg cgatggtgtc
      901 gaaactcaat gacacaaaac ccgggcgcac gctgcggtgg cggtaaagca tggtgtccac
      961 gtcgatttcg gtcaggctgc cgccgttgcc gatgatgtct tcaaactcgg cgaagaaacc
     1021 gcacaacgcc gcgccgtcgt gttccatcgc ttcgcggatg cgggcgatgt cggcttcgga
     1081 tttgcaggat ttgaacagcg tggacgggtt ggtgccttcg attaggcgta cgctttcggg
     1141 caggcgcacg agcgtactga cggcggtttt gttcggttcg ataagcagcg cgccgccgat
     1201 ttgcgcgagc ttgtcggcaa cttgggcgta aggttcgacc gtgatgccgg cggtttgcag
     1261 cgcggcggcg gcttcggcgt tcagacggca ttgttcggta aacaggacgg cgttgtcttt
     1321 gccaatcagc aggaaggaca cgaaaacggg attgaaaggc acgtcgctgc cgcgcaggtt
     1381 ggtcagccaa gcgatgtcgt caagcgatga aaccaagtgg taatccgcgc ctttttccgc
     1441 catcacggcg cgcacgcggg cgagtttttc ggcggcggtt tcggagacgt aggcggggtc
     1501 gtggatgaac acggtttcgg cggggatagc gggacggctt gtccacactt ggtcaagcag
     1561 atcgtccggg tgttcgatgc ggatgttttt ggcggtcagg gattgcgcca aagtgcgttt
     1621 gccggtgagc gagaccatat cggaagggat gccgacggcg gcgttttcgg gcaggtttgc
     1681 cgcgagccat tcgttatacg gcggcacttg cccgcttttt tgcagttcga tgccgctgcc
     1741 cgcaagctgc ttggcggctt gttcccaata gcggctgtcc acccatacgc ccgcttcatc
     1801 ggtggtcagg acaaacgtgc cgaccgagcc ggtaaagccc gataattcgc ggcgcgcctg
     1861 ccaatgctcg ggcaggtatt cggacaggtg ggggtcggcg gaagggatga cgagcgcgtc
     1921 caagccttgc gccttcatgg cttcgcgcaa tgcggacagg taattcgata cggtattcat
     1981 aacagttcct ccaatcgggt tttgcggctt cagacggcat ggacggaaat ctgaaatgcc
     2041 gtctgaaaaa cataaccatc ttatcaaatc gccagcctgc tgcaaaacgg acgggctgat
     2101 cggatggtgc gtgtttgcgc gaaaaaagcg atgtaaggtt gtttgttgta aaattttagg
     2161 cataaaatgt cgttttatgc ctttttttgg aagtaatatg gaaaatatag tcaagtttga
     2221 gcatgaagtt ctaatagtgt gtgttggcag gtatttggat tacgttacaa tacgtgtttt
     2281 catcaaaatt tccctaccga gcggtatcct gtttttgaag acggtaggat ttgggtttgt
     2341 ttgggggcaa tcccgcccaa attggagcaa tagatagggc tgtgtccgaa tatgcggctc
     2401 tgtgtgttga aacacagtta aaaatacgga agactttatg tccgaacaac atatttcgac
     2461 ttggaaaagt aaaatcaacg cattgggtcc ggggatcatg atggcttcgg cggcggtcgg
     2521 cggttcgcac ctgattgcct cgacgcaggc gggcgcgctt tacggctggc agatcgcgct
     2581 catcatcatc ctgaccaacc tcttcaaata cccgtttttc cgcttcagcg cgcattacac
     2641 gctggacacg ggcaagagcc tgattgaagg ttatgccgag aagagccgtg tttatttgtg
     2701 ggttttcctg attttgtgca tcatttccgc cacgattaac gctggcgcgg tcgccatcgt
     2761 aaccgccgcc atcgtcaaaa tggcgattcc ctcgctgatg tttgatgccg gcacggttgc
     2821 cgccttgatt atggcatcct gcctgattat tttggtgagc ggacgttacc gcgctttgga
     2881 tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc acgcttgccg ccgccggcat
     2941 cgctatgtcg cgcggtatgc agatgcagcc ggactttatc gagccgacac cgtggacgct
     3001 tgccggtttg ggcttcctga tcgcgctgat gggctggatg cccgcgccga ttgaaatttc
     3061 cgccatcaat tctttgtggg taaccgaaaa acaacgcatc aatccttccg aataccgcga
     3121 cgggattttt gatttcaacg tcggttatat cgccagtgcg gttttggctt tggttttcct
     3181 tgcactgggc gcgtttgtgc aatacggcaa cggcgaagca gtgcagatgg cgggcggcaa
     3241 atatatcggg caattgatca atatgtacgc cgttaccatc ggcggctggt cgcgcccgct
     3301 ggtggcgttt atcgcttttg cctgcatgta cggcacgacg attaccgttg tggacggcta
     3361 tgcccgtgcc attgccgaac ccgtgcgcct gctgcgcgga aaagacaaaa cgggcaacgc
     3421 cgaattcttt gcctggaata tttgggtggc gggcagcggt ttggcggtga ttttttggtt
     3481 tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt gccgcttttg tgtccgcccc
     3541 tgtgtttgcc tggctgaatt accgtttggt caaaggtgat gaaaaacaca aactcacatc
     3601 aggtatgaat gcccttgcat tggcaggctt gatttatctg accggcttta ccgttttgtt
     3661 cctattgaat ttggcgggaa tgttcaaatg ataacgatgc cgtctgaaac cgcaaaccgc
     3721 tttcagacgg cattgccgcg tttatggaaa aaaacaccga catcgcgtaa aatatgcgca
     3781 aattttgtga ttcggtcagg tcgtctgaaa cagattgcgc ctgatttatt ttttcggaaa
     3841 accctatgag cgaacaaaac catccgcaaa ccgagccgca gttggacgaa aaccaaatca
     3901 tcgccctgcg ccgcgaaaaa ctgcacaaca tccgccaaca gcgcaacgcc taccctaacg
     3961 atttcaaacg cgacagcttt gccgccgacc tgcataccca atacggtgaa atcggcaaag
     4021 aagaactcga cccgcaaggc attcccgtca aaattgccgg ccgcatgatg ctcaaacggc
     4081 aaatgggcaa ggcgagcttt gccaccattc aagacgtgtc cgggcaaatc cagctttatc
     4141 tgaacaacaa aggcgtgagc caagaaattt tggacgactt caaccattgg gacttgggcg
     4201 acatcgtcgg tgcggaaggc actttgttca aaaccaacca cggcgaactg accgtacgcg
     4261 tgtccggcat ccgcctgctg tccaaatccc tgcgtccgct gcccgacaaa cacaaaggtt
     4321 tgagcgatca ggaaaccaaa taccgccaac gctatgttga tttgattgcc aacggagaat
     4381 cgcgcaatac cttcatcaaa cgcagccaaa tcatccaatc cgtgcgtaat tttatggtgg
     4441 gcgagcatta tctcgaagtc gaaaccccga tgatgcaccc gattcccggc ggcgcgacgg
     4501 caaaaccctt cgttacccac cacaatgcct tggacattcc gctctacctg cgcatcgccc
     4561 ccgagctgta tctgaaacgc ttggttgtcg gcggtttgga gcgcgtgttt gaaatcaatc
     4621 gcagcttccg caacgaaggt atgtccgtgc gccacaatcc cgaattcacc atgatcgaat
     4681 tctacgaagc cttctccgac tacgaacgta tgatgcagat ggcagaagac atcatccgca
     4741 acgcatcgcg cacagtaaac ggtacggcaa acatcactta caatggcaaa gaagtcgatt
     4801 tggaaagccc gttcgagcgt ctgaccatcc tcgaagccat caaaaaatac aatccgcact
     4861 acaccgacga gcagttgaac gatgcggaat ggctgaaaaa agaaatcgtc aaacacggtg
     4921 aaagcctgcc gccgtccccg ggcatcggca gcctgcaact cgcgctgttt gaaggttgcg
     4981 ccgagggcaa gctgtggaac ccgaccttca tcatcgatta cccggtcgaa gtttccccgt
     5041 tggcgcgcgc ttcggatacc aaacaaggtc tgaccgaacg tttcgaattg ttcgttgtcg
     5101 gccgcgaact ggcaaacggc tattcagaat tgaatgaccc tgaagaccaa gccgaacgct
     5161 tcaaatcgca agtcgcacaa aaagacgcgg gcgacgacga agccatgcat tacgatgccg
     5221 actacatccg cgcgatggaa tttggtttgc cgccgaccgg cggttgcggc atcggtatcg
     5281 accgcttggt gatgttgctg accgattcgc aaaccatccg cgatgtgatt ctgtttccgc
     5341 agatgcgccc cgagtagtca taaaaacagt tgaaatggca atgccgtctg aacccgattg
     5401 gattcagacg gcattttgta tggcggtacg gatttattcg ctttctaact gaccgaccca
     5461 ttcggacaag gcagtcaggc gttgttcgga tttcgccacg aacgggtttt cggtatccca
     5521 cgcgtagcct gccaaaatcg aagaaagcat acggctgatt tccgggctgc ggtcgggcgc
     5581 gtacacgacg ttttggaagc ggaaatcgta ccaaccgtcc acacatcagg ggtggtaatc
     5641 cgaatcaaca cgccttctcg cctgtgcggc ggacataaac cgttttttga cctccagcac
     5701 cacacctaaa atcgtgctgc cttcgggcag gtcaaaggct tcagacggcc tgcccgtctg
     5761 ataaatcagc atcctgcccg caatatcgat aacaaggtgc gcgacgacca gccgcttgtc
     5821 ggacagttca atcagcacga tgtcctcgtc cgcagggcgc atacgcggct cgaccaccac
     5881 caaatcccct ttttcgataa ccggcgacat ggacgtgtcg ggcatggcaa cggcaaacgt
     5941 gtactgcgag ggagcgacca ccgaagcaaa caagtcgaaa ctgcctgtga gtttgcctgt
     6001 acgggcgaaa gccgccgcct gacggtctga caacaggggg cgtggtgtga gcctcatcga
     6061 cagtgctttg ccgtcggcat cgctgtggtt gtgcgtatgc gtatgatggt tgttgatggt
     6121 cgtatcgcct tggacatcgt taaggactat gctgtttgaa cctgccacgg atttggtcat
     6181 ctcattgtag gcttgcgtcg tgatgcgctc ttgcttggtc atacctgtta cagcctgttt
     6241 cagcatttct aacgctatgt gattcttcct aagggtcgct ataccggctt ttgcagcttg
     6301 ctcattacca caagaggggc aaccccagct acttcctatg aacatattgg agtacctcga
     6361 acaggtttgg tttccatgtt tagggcagtt gattacacac ggcttggctg taccctcaaa
     6421 ctcgaccaac ttaatgccac gatgaccgaa acgttcctca acctcagcag cagcttcttc
     6481 aaatgttttt gaccttcttg ccatattgac ctccggatta ttgattattt tggctatgct
     6541 ttaacgaggg attggaatga ggtaaagctg ttgccctctc cccgcaatag ttccattagg
     6601 cgcggatgaa tgaatagttt gtccctgccg atgacgattt cttgcagcac acctatgtct
     6661 gaaagctctt tcaggtactt agaggccgtc tgccgtttgg ctatccctgc cgcttctagg
     6721 ttggcaatgc gtgtatatgg ctgctcaaac agaagattta ccagttcgtg cgtgtagatt
     6781 ccttgtgcgt gtgtccgtat gtgttgccgt gtctgctcga acaggcggcg tatcgcatct
     6841 attttcgata ccgtccaatc ggcggtatcg gctacgccgt ctaagatgta gattatccag
     6901 ctttcccaat cctgccgttc ggttacgcct aaaagcaggc ggtaatagtc cgccctgttt
     6961 tcgatgatgt agcggctcaa atacaaaata ggcaaatcca aaagcccttt ttcaatcaat
     7021 agcaggctgt tcaatatgcg ccccgtccgc ccgttgccgt ccgtaaacgg atggatggct
     7081 tcaaattggt aatgtgccgc cgccatgatg ataagcgggt ctaaatcgcc gctttcgtga
     7141 ataaaccgct cccaatttgc cagcttgccg cgtatggttt cttctccttc gggcggggta
     7201 tagacaacat ttccgctgtt gcctcctttt agggctgtgc cgcctgtttt gcggatggcc
     7261 atttcgtagg ggtgcttgat ggcgttgcag accatgatgg cggtttgtgt gcataaaggg
     7321 cggctcgtca gtgattcata gcctgcaaac agggcggtgc ggtattgcag ggcttctttc
     7381 gtggcagggt cttgccgttc cgtatccatt tgcagggatt gaaacagctt gtccgttgtg
     7441 gttacgatgt tttcaatttc cgaacttgca cgggcttcca taacaggaag ggtgttaatc
     7501 agcatggctt gattcggtat cagttctgcc gcctgcttta aacgggcaag ggatgcacgg
     7561 gcggctatac aacgtttcag gatggttttg ctttcaatat cctgttttgg cggcaggggt
     7621 ggtaaatcgt tatagggaat atcgggtttc cagttgctca tatttaaaat ttcaaaaaat
     7681 ttaaagatgt ttccagtata tgtttacgcc gtgtatatat caaggatata tgtttaaaaa
     7741 tttggctttt gtaaatatat gggcggtaaa accgcattat tttatgctgt tacctatttt
     7801 acaccttgtc tgcacacgat ttccaaacaa cctaaaaaga aacctgccga aagacaggtt
     7861 taaagaggtc attttaaatg tgaactaaag tttacaccct cggagggtat cgcgtatatc
     7921 agacgtactt aatatacact atttag
//
LOCUS       DAWHXS010000087         7900 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWHXS010000087 DAWHXS010000000
VERSION     DAWHXS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.087"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(421..2697)
                     /locus_tag="WIE22_001586"
     CDS             complement(421..2697)
                     /locus_tag="WIE22_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4127646.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     NKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSRADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFKNKTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            3119..3709
                     /locus_tag="WIE22_001587"
     CDS             3119..3709
                     /locus_tag="WIE22_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ4127647.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRDNLPDCLEITAWTDDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            3772..4830
                     /gene="trpD"
                     /locus_tag="WIE22_001588"
     CDS             3772..4830
                     /gene="trpD"
                     /locus_tag="WIE22_001588"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ4127648.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4982..5197)
                     /locus_tag="WIE22_001589"
                     /pseudo
     CDS             complement(4982..5197)
                     /locus_tag="WIE22_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5299..6642
                     /locus_tag="WIE22_001590"
     CDS             5299..6642
                     /locus_tag="WIE22_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ4127649.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            6687..6977
                     /locus_tag="WIE22_001591"
     CDS             6687..6977
                     /locus_tag="WIE22_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127650.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQPVKKG"
     gene            6982..7179
                     /locus_tag="WIE22_001592"
     CDS             6982..7179
                     /locus_tag="WIE22_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127651.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            7187..7471
                     /locus_tag="WIE22_001593"
     CDS             7187..7471
                     /locus_tag="WIE22_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127652.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            7478..7756
                     /locus_tag="WIE22_001594"
     CDS             7478..7756
                     /locus_tag="WIE22_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127653.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT         2018 a         1813 c         2071 g         1998 t
ORIGIN      
        1 ccaaatgcta aggcgattta tcggaaacgg ctgaaaccga acgaattgga ttcccgcctg
       61 cgcaggaatg acaactcatt agttacctaa aacttaaaaa acagaaacct ttacaccgtc
      121 attcccacga aagtgggaat ctagaaccca aatgctaagg cgatttatcg gaaacggctg
      181 aaaccgaatg aattggattc ccgcctgcgc gggaatgacg ggatcttggg tttctgcttt
      241 tgatttttct gcttttgcga gaatgacggc gtgaaagtaa gaatgatgaa acaaaaaaaa
      301 tgggaatgat ggcatagtgg tttgttcttt gtctttgcca tatttcctaa caaattgatt
      361 aaaaagaaaa aaggttttca gaatgccgtc tgaaaacctt ttttgtttgc ctgtccgatt
      421 ttaaaacttc acgttcacgc cgccggtaaa gctgcggccc atttgcggcg tatcagagag
      481 aaagctgctg tgggcgtaaa cggattggtt gagcaggttg tcggctttga cgtaccaatt
      541 ccactcgcca tagcgcgtat tgcggcggta gtttgcgccg aggttgagca tatggtgtcc
      601 gggcgtgcgc gtttcgtagc gggcgagttt gttttgggca aacacgcggt agtagtccaa
      661 attggcatcg atgcggtcgg tcagcgaggc tttcaggtgg aagccgaggc gcgcagccgg
      721 aacgcgaggg gcgttttggt cgtcctgcgc gatgaaagga cggttgccgt aggcatcttc
      781 cctgccgggt agggacggca ggtttttcag acggcctcgt acatagtcgc cggaaacgcc
      841 gatgcggtag cgcggtgtcg gtttgaagta gatttcgcct tccgcgccgt agaagtccgc
      901 accggattgg ttgtagcgca cgagcttcat ttcgctgtcg tcttcgatgg atttggggcc
      961 gcgtccgtcg tttaaggttt gggcgtaaat gtagttgccg aagcggttgc ggtagagtgc
     1021 cagattgtat tgccagcggt cgccttcgta gcccaacgcg agttcgatgt tgttggaacg
     1081 ctctttgttc aggtgtttgt tgccgacttc aaaagtgttg gtggcaacgt gtttgccgtg
     1141 tgcgtacagc tcttgcgttg acggcaggcg ttcctgatgg gaggcggtca ggctgagttt
     1201 gtgttgcggc gtgaaatacc agttgcccga aagtgcgaac gagcgggcgg tttggcggtg
     1261 cgcgccgagg tcgggcaggg ggtggttgta gtagttttcc cgatcaatca atgctttgtc
     1321 gtaccggatg gaggcttttt gtttttccac gcgtacgccg ccttcaagcg tgaagttgtc
     1381 ccagtttgcc tgttctacac cgaaaaagct gtaatggcgc acattgttgt caatcaacat
     1441 cggttgtttg accgcttcgg atgtggcaga taaagcactg gatttttgtt gtaaatattg
     1501 cacgccccag ctgcctttca gacggcctat gggttggtgg cgcaactcga tgcgggcgtt
     1561 ttgcgttttg tttttgaaga agttttccac tgcatcgcct gctttttcgt cgtggcggta
     1621 gtcgttgcgg ttcaagtata cgcgcagggc ttcaaaaccg gggaacggtt gcttccattc
     1681 ggcacggagt tcgtagcgtt tgttgcgcag gtctatccac ggtctgccgc tgtgggtgtg
     1741 tgcgtgtgca ttatcgtcgt cgtggaagcc gcagctcaag cccggattgt cgtaatcgat
     1801 gtcttcttcg gtcaacaggt gcggataaag ctgtaaatag cgtttgttaa tcaagctctt
     1861 ttgccagatg atgtcggcgt ggcaatcatc gtattcgtgg ctgtgggcag gcagaccata
     1921 ttggtcgcga cggtcgctgt acgctacgcc gataaaacct ttttcgccaa cccaagacag
     1981 cccgatgctg cccgtttgcg aatcggcgcg gctgtcgggc aggcgtttca gattgcggta
     2041 acgcggtacg gcgtaatcgc ccgatttgcg gtacaagcct tccgtatgca atacaaagtt
     2101 tttgcccaag ccgatattga tgcctgcgga tgtcagtttt tccaaattgc cgctgctcaa
     2161 acgcaatccg gcttcgcccg atacgccgtt ttcaggcatt ttttcgggaa ttttattgtc
     2221 ggcaacatca accagccccg cgacattgcc cgaactatat aaaagcgtaa ccgggccgcg
     2281 caggatttcg acctgttgcg acaaggcggt atctaccata atggcgtgat cgggcgaaaa
     2341 atccgccata tcgcccgttt cgccgtgatg gttcaaaacc ttaatccgcc tgcctgtttg
     2401 accgcgtatc accggtgcgg acgcgccgcc gccgtattgc gaggcgtgaa tacccggtac
     2461 gccgtccaaa gcatcgccta agttgacggc tttttggcgc aaggtatcgc cggagatgat
     2521 tttgtcggag gcggtcgaag tgtgcagcag ccccgaagtg gcgcgcggac ggcttttgcc
     2581 gacgacgctg accgtttcca aatccaccga ttgctcagtt tcatgcgctt gggcgaggag
     2641 gggtgtgttg attaaaagaa ttgataaaac aatgggtttg agtgtagttt gtgccatttt
     2701 ggtttctcgt cgcatttcaa aagtttgtta ttatataaca ttacattcct tatatcataa
     2761 gattttgaga acactcagag ggcataggca aaagtttttc aaatgaaacg gttgcggcat
     2821 cgggcggtgt ccatttgtat ccgccgtcct tcgggggcgc ggttgatgtt gacgcagatt
     2881 ccgctgtgtt tgcccattat gttcggctgc gggataacca aaatttatga gtgcataaaa
     2941 acggtacgtt cccgaacggt cgaaaaggtg gcaaaatggc gtacttgtca gaacgcgagg
     3001 ctctgcgcca ggttcacgag ggtgcatcgg gcacgctaag atataagagg gtatggatgg
     3061 gggtatcgga aatgcagtta ataacaaaca aattataaat caataggtta ataacaaaat
     3121 gcttttgttt atcgacaatt acgacagttt tacttacaac atcgtccagt atttcgctga
     3181 attggggcag gaagtcgccg tgcgccgcaa cgatgatatt acgttggaag aaatcgaggc
     3241 attgaatccg caatatctcg ttatcggacc cggtccgtgt tcgcccaaag aagcggggat
     3301 ttccgtggcg gcgatgcgcc attttgccgg ccggctgccg attatgggcg tgtgcctcgg
     3361 gcatcagacg ataggcgagg cgttcggcgg caggatagtc cgcgccaaaa cgctgatgca
     3421 cggtaaggtg tcgcccgtgt cccattcggg caagggtatg tttaaaggtt tgcccaatcc
     3481 ggttacctgt acgcgttatc acagcctcgt tatcgatcgg gataatttgc ccgattgttt
     3541 ggaaatcacg gcttggactg acgacggcga gattatgggc gtgcgccata aggaatatgc
     3601 cgtcgagggc gtgcagttcc accccgaagc cctcttgacc gaacacggac atgatatgtt
     3661 gaacaatttt ttaatcgaat ttcaaaactt caaaccgcaa aaaatctgac gcgatgccgt
     3721 ctgaaggctt cagacggcat tttcgtccga atattgaacg gaggacaaaa aatgattaca
     3781 ccgcaacagg ctatcgaacg attaatcagc aataacgagt tgttttacga tgaaatgacc
     3841 gacttgatgc gtcagattat gagaggacag gttctgccgg agcagatagc ggccattttg
     3901 acaggattgc gtatcaaggt tgaaaccgtt tccgaaatta ccgcagctgc agccgtcatg
     3961 cgcgagtttg cgacaaaagt gccgctggag aatgcagagg ggctggtcga tatcgtcggt
     4021 acgggcgggg atggtgccaa aaccttcaat atttcgacga cctcgatgtt tgttgccgca
     4081 gcggcaggcg cgaaggttgc caaacacggc gggcgttccg tttcatcttc cagcggcgcg
     4141 gcggacgtgg tggagcagat gggcgcaaac ctcaacctga ctcccgaaca gattgctcaa
     4201 agtatcagcc agaccggtat agggtttatg ttcgccccca atcaccacag tgccatgcgc
     4261 catgtcgccc cggtacgccg ttcgctcggt ttccgaagca ttttcaacat attgggtccg
     4321 ttgaccaatc ctgcgggcgc gccgaaccaa cttttgggcg tgttccacac cgatttgtgc
     4381 ggcattttgt cgcgggtctt gcaacaactt ggttcaaaac acgttttggt cgtttgcggg
     4441 gagggcggct tggatgaaat tacactgacg ggcaaaacgc gcgttgccga actcaaagac
     4501 ggaaaaatca gcgaatacga catccgccca gaagatttcg gtatcgaaac ccgccgcaat
     4561 ttggatgaaa tcaaagttgc caatactcag gaatctttgt tgaaaatgaa tgaggtgctg
     4621 gatgggaaag aaggggctgc gcgcgatatc gtattgctta ataccgccgc cgccctatat
     4681 gccggaaatg tcgccgcttc gctttcagac ggcatatctg ccgcacggga agccatcgat
     4741 tcaggcaggg caaaatcgaa aaaagaggag tttgtcggtt ttcaaccaca acaaagatgc
     4801 cattttcttg gaaagatgga gcttgggtga tgccgccatg attatggaac ttttgtggca
     4861 aaacataagc acttcacgaa gagaacttac caaactgttt ttatataaaa acttggggct
     4921 gtactagata accagaccaa attcccatta actaattgtc ttaaaatctg aatttgagat
     4981 tctatttaaa atgccattgg catttcttta aatgcagccc caaatgctct ttgggaatgc
     5041 cgttaaactt acgtaaatgg ctaaattcac taatatcaag cacatcataa ctacgaaagg
     5101 tatccgtgta tacaatgcca tcaggcttaa ctttctttcg gataattggc aacaatgtcg
     5161 ctgattgcgc attaggaaca acgacggtat aaaccttata tatttcgtcc ctaagaaggg
     5221 acgattaaca aaaattaacg ccctttactt tctacaagta acagggcttt tttttgcccg
     5281 tttttgagga ttcgcaccat ggaagataag caagggatga caaaggcggt tgtcggcgtg
     5341 atgacggacg cgttagcgga cggcaggaag ccggcaaccg cttcaaatct tcccccctta
     5401 tctaacaggg ggggtacaga aaccgaaacg gcaggcaggg ttcaggaagt cttcgaatgt
     5461 tacgaaacgt acataacgga cggtaaagga aacctgttag gcgttcctct tcggcgcggt
     5521 gtatcagatt cggctttcat tgatcaaatt agcttttcat ttcatgaaaa aacctttttc
     5581 gataaatacg gcgttcgtgt aagtcttttg gaagacgaag atttcattcg cgccgcgtcc
     5641 atgctcgccg aagaagtttt cggtttcggt atctacaaag agtccaaagg ctcgggcggt
     5701 cgtttttatg agcgctgttg gttgatgggt tcggaagacg tcctatacgg tcgcgtccat
     5761 tttggcggac aaaacaatac catccttttc gaactgaccg gcaccggttg cggcgtcgca
     5821 aaagaaggct gggaatcacg acttttcgca ttcctgacta atgcaatccg cccaaaaatc
     5881 acacgcgttg acatcgcaaa agactttttc aacggcgaat acagcccgaa ccaagcccgt
     5941 gaagaccgaa ataaaggtat gtttacctgt catcacgtca aaccaaaagg cgaatgtttg
     6001 gggtcagatt gggaagaaga cgatgaagcc aaaatgacca aaggcaagac ctatggtatc
     6061 ggctcccgtg aatcgtccaa atatgtccgc gtctatgaaa aaggcaagca gttgggcgat
     6121 aaaacaagca catggacgcg ctttgaaatt gaattcaaag caaaagacat cgttatccct
     6181 tttgaagttt tgcagaatcc gggcgaatat ttcggcggcg catatccgat ttgcgaacga
     6241 ttcgtccaaa aggcaacgcg catacacgcg gttaaggaag ataaggtcat ttcagccgac
     6301 cgctaccttg aatgggtaaa aaaacagttt ggacgtgcgg caaacggtct gaaattcatt
     6361 tttcccgaat tggacaaagc caaactgttt gaactgattg agccgagtca tcacaagctg
     6421 cccaagtctt tggctcccga agcctacgac tgcgcctttt tgaaagctca agccattcat
     6481 gaacagcccg cattcaaacc gtacaaagac ccttactata tgtacgaata ttacgagaat
     6541 cttgaaaaac agcttgaaca gcaaaaacac gtcaacaatg aagaaagcta taacaacttc
     6601 atttacgaca aattcgcaag actaccgatt tcatgggctt aaagtgtctg cccgaaagac
     6661 gtttaatcac acaaggaaac caaaaaatga acatccaact tcaaggccac atcgtcggcg
     6721 ttaaaaaatt caacggacaa atcgaaggca agagcttcga ctattgccgc ctgattgtcg
     6781 ccacaccctt agacagctcc caaggcaacg cattgggcag ctctactact gaatacgatt
     6841 tcggcggctc tgccaatttc gagcagttcc gaaacgccca atttccgatc gaagcaaacc
     6901 tgaacgtaga aatcgtcact acgggcaaaa cccaaaaact gaaagtcatc ggttttcaac
     6961 ccgttaagaa aggctgattg aatgcagaaa gtctatgttg tccagtccgt atcaacaggg
     7021 gactttctgt atctctctcc tgaaacgggc gacatcggac ataccaaatt aatcaccaat
     7081 gccgattatt tctacgactt cgaagaagcg attaacgcag gtttggaaga aatcggcaac
     7141 caatacgaat ttgtcgtatt cggatttttg aaagactgat tttcggatgt tcggcggtcg
     7201 tctgaaaaac gctccatcca ttaccgccaa acactttttg aaggaaaata tcatgaaatt
     7261 tattaacacc tgccgtaaat acggcgcaaa actggctgtt gtaacagctg ctcccctggc
     7321 tttggccgca catgcaaatg caacgttgcc cgatacagca aaaaacgctt tggaagccgc
     7381 aaaagcggac ggtatggaag ccggttggat tgtcgttggc gttttcgccg cgctttttgt
     7441 attttccatc gttaagagag tgatgaagta agacggcatg tactaccaag tcggaaataa
     7501 atgtcttgag aagcaccagg ctgaaaacct ttatttcagc ttggtagtac caagaatcaa
     7561 agaaaacgga cagattgtca ggccggaata taacggcagt ctgtggaaga tgtcggacgg
     7621 tcagccgcta aggcttttat tggcggaatg cagtccgaaa gacaacctgc aaagcggtct
     7681 tgaaacaggc tggatagtat tcggcatcct cgcgtccgtt tactttgttt ccctgctgaa
     7741 aaaggttttg aaatgatgga tttttatttt tatctcggcg tttccgtacc cgtattaatc
     7801 ggggcggttc tgtttaagaa ttgagcgcat gaagttatgg tgtcaaaatc aggctttcaa
     7861 aacaatcatt gaaaggcaga accatgaaca agccgtttat
//
LOCUS       DAWHXS010000088         7895 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWHXS010000088 DAWHXS010000000
VERSION     DAWHXS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.088"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..108
                     /locus_tag="WIE22_001595"
     CDS             <1..108
                     /locus_tag="WIE22_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEZ4127654.1"
                     /translation="VPKKAETISNKLFFTHHPKRQTEFAVRQIRQARAE"
     gene            234..1529
                     /gene="hisS"
                     /locus_tag="WIE22_001596"
     CDS             234..1529
                     /gene="hisS"
                     /locus_tag="WIE22_001596"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ4127655.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            1530..2159
                     /locus_tag="WIE22_001597"
     CDS             1530..2159
                     /locus_tag="WIE22_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ4127656.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            2313..3770
                     /gene="der"
                     /locus_tag="WIE22_001598"
     CDS             2313..3770
                     /gene="der"
                     /locus_tag="WIE22_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ4127657.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3801..4700
                     /gene="rdgC"
                     /locus_tag="WIE22_001599"
     CDS             3801..4700
                     /gene="rdgC"
                     /locus_tag="WIE22_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ4127658.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLEWFSEGF
                     AAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAASLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            4752..5168
                     /locus_tag="WIE22_001600"
     CDS             4752..5168
                     /locus_tag="WIE22_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127659.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYDGGVGKDRLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            5234..5800
                     /locus_tag="WIE22_001601"
     CDS             5234..5800
                     /locus_tag="WIE22_001601"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ4127660.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5864..6568)
                     /locus_tag="WIE22_001602"
     CDS             complement(5864..6568)
                     /locus_tag="WIE22_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ4127661.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEAVQDTMSA
                     RLQVAAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGTSGYKIVKLNLGHIGSHIAGEGAAQAKMLRAMPMAASYGVEGTDSAA
                     PGVEEISISVNGTVQF"
     gene            6714..7895
                     /locus_tag="WIE22_001603"
                     /pseudo
     CDS             6714..7895
                     /locus_tag="WIE22_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271049.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2099 a         2175 c         1895 g         1726 t
ORIGIN      
        1 gtgccgaaaa aagccgagac aatttcaaat aaattgtttt tcacacatca tcccaagcgg
       61 cagacagagt ttgcagtccg acaaatcagg caagcacgtg cagagtaagt caaatgccgt
      121 ctgaaaactg tttcagacgg cattttaccc gtccgcaaac cacccccaac ctcacaaacc
      181 cttaaaaatc cgctataatc gcccgcaatt tgatttaagc attcttactt gaaatggcac
      241 aaaaaatcca atccgtcaaa ggcatgaacg accttctgcc tgtcgagcaa aaagatttca
      301 aactgacggc tgcgttttgg caggcgtttg aggatacggt aaaccgctgg acgcgcgctt
      361 acggctatca gcaaatccgt acgccgattg tcgagcaaac cggtttgttt gtccgctcca
      421 tcggcgagga aaccgatgtg gtcggcaagg aaatgtacac cttttccgat tcaaacgatt
      481 ctttgagttt gagcctgcgt ccggaaggta cggcttcttg tctgcgtgcg gtggtcgaac
      541 acaaccttct gtacaacagc ccgcaaaagc tgtggtatat ggggccgatg ttccgccgcg
      601 agcgtccgca aaaaggccgt tatcgtcaat tccatcaggt cggtatcgag gctttgggtt
      661 ttgaagggcc ggatatcgat gcggaaatca tcgcgatgtc tgccgacttg tgggaaaaat
      721 tgggtatccg cgaatacctg actttggaaa tcaacagctt aggcaaccgt gaggagcgag
      781 cggcacaccg tgcggcgttg gttgaatatt tgacacgcta tgaagataaa ttggacgaag
      841 acagcaaacg ccgtctgaaa accaatcctt tgcgtgtttt agatacgaaa aaccctgatt
      901 tgcaggaaat ctgcaacgcc gcgccgcgtt tggtggatta tttgggcgag gcttcgcaaa
      961 accactatgc acgcttcaag gcgatgttgg acggtttggg tatccaatat attgaaaatc
     1021 cgcgcttggt tcgcggtttg gattattaca atcagacggt ttttgagtgg acgaccgaca
     1081 aactcggcgc gcaggcaact gtgtgcggcg gcggccgtta cgatggtttg attgaggaac
     1141 ttggcggcaa acctgcgccg tccatcggct ttgcgatggg catcgaacgg ctgctgcttt
     1201 tggtgagcga atacggctcg ctggaagtga acgctgcgcc tgatgtctat gcaatgcacc
     1261 aaggcgaaag ggcggacttg caggtgatga aatacgcgca agccttgcgc gcgcaaggtt
     1321 tcaatgtaat gcagcattcc ggctatcaaa gcctgaaagc gcaaatgaaa aaagccgaca
     1381 acagcggcgc gcgctttgcc ctgattgtcg cgcaagacga actggcgaac ggtacggtta
     1441 cgctcaaaga catgaacggc gcacacggtc agcaaactgt cgccgccgag gatttaaccc
     1501 atactttaca acaatggaag aacgcataaa tggcagccca tctcgaagaa caacaagagt
     1561 tagacaactt taaatatttt tggaaaacca cgggcaaatg gctgtttgcc ctgctgattt
     1621 tggcagcact cggctacttg ggatacacgg tttaccaaaa ccgtgcggct tcccaaaatc
     1681 aggaagcggc ggcggtgctg gcaaacatcg tggaaaaggc gcaaaacaaa gccccgcaaa
     1741 gcgaaattaa tgccgaattg accaaactcc aacaaagcta cccgcattct atttccgccg
     1801 cccaagccac gctgatggcg gcggcaaccg aatttgacgc gcagcgttac gatgttgccg
     1861 aaggccattt gaaatgggtg ttgtccaacc aaaaagacag cctgattcaa gcgttggcgg
     1921 cgcagcgtct gggcgttgtg ttgttgcaac aaaaaaaata cgatgccgcg cttgccgcgc
     1981 tcgacacgcc ggttgaagcg gacttctccc ccctgctgat ggaaaccaaa ggcgatgtct
     2041 atgccgcaca gggaaaaagc caggaagcct taaaaaacta cggtcaggct ttagaaaaaa
     2101 tgcctcaaga ttctgtcggt cgtgaattgg ttcaaatgaa actcgattcg ctgaaataaa
     2161 tgccgtctga aatgccgtcc attatttatc agacggcatt ttaccctccc tctttatggc
     2221 ctcttgccgt gaagaaccta tttaccgtgt ccaacggaca cctgctcgtg ttcagttctc
     2281 ccgaacggca cttgacacag aaagactcaa tcatgaaacc aaccatcgcg cttgtcggcc
     2341 gccccaacgt cggcaaatct actttattca accgtttgac gcgcaccaaa gacgcactcg
     2401 tacacgacct gcccggcctg acccgcgacc gccattacgg acacggcaaa gtcggcagca
     2461 aaccttattt ggtcatcgat accggcggtt tcgagccggt tgtggacagc ggcattttgc
     2521 acgaaatggc aaaacaaacc ttacaggctg tcgatgaagc cgatgcggtt gtgtttttgg
     2581 tggacggccg taccggtctg acgccgcaag ataaaatcat tgccgaccgt ttgcgccaaa
     2641 gcccgcgccc cgtttatttg gccgtgaata aaggagaagg cggcaataga gccgtgcttg
     2701 ccgccgagtt ctacgaactt gctttgggcg acccttatgt tatttcaggt gcgcacggtg
     2761 acggcgtgta ttatctgatt gaagatattt tggaaacctt ccctgagcct gaaaaagaag
     2821 aggaagaggc gaaacatcct gtttttgccg ttatcggtcg tccaaatgtc ggtaaatcaa
     2881 ccttggtaaa cgccattctc ggcgaagagc gcgtcatcgc cttcgatatg gcaggtacga
     2941 cgcgcgacag tatccatatc gatttcgagc gcgaaggcaa accgtttacc atcatcgata
     3001 ccgcaggtgt gcgccgtcgc ggcaaagtgg atgaagcagt ggaaaaattc tccgttatca
     3061 aagcgatgca ggcggttgaa gcggcaaacg tcgctgtttt ggtattggac gcgcagcagg
     3121 acatcgccga ccaagatgcg acgattgcag gtttcgcttt ggaggcaggc cgtgctttgg
     3181 tggtggcggt caataaatgg gacggcatca gcgaagaacg tcgcgagcaa gtgaaacgcg
     3241 atatcaaccg caaactgtat ttcctcgatt ttgccaagtt ccactttatt tccgcattga
     3301 aagagcgcgg tatagacggt ttgtttgaca gcattcaggc tgcctacaac gcggcgatga
     3361 ttaagatgcc gacgccgaaa atcacgcgcg tattacaaag cgcgatcgag cgtcagcaac
     3421 cgccgcgtgc cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc ggcatgaacc
     3481 ctcccgtaat tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc tatacgcgct
     3541 atctgaccca aacgttccgc aaagccttca atctgcaagg cacgccgctc agaattcaat
     3601 acaatgtttc ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa aaaccgctgc
     3661 gccgcgtcag cctgagcaac cgtattgaga aacgcgaagg ccgcaaagaa gagaaaaacc
     3721 gcttcaagaa gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa ttcccgacat
     3781 ttcaaacaat aggaaacatt atgtggttca agcagattag tttttatccg ctcaacaaag
     3841 aaaagctgcc tgaggcagac gtacttgccg acaaacttgc tgaagctgaa tttacccatt
     3901 gccaaggctt agagtggttc agcgaaggct ttgccgcacc ggtttcgttc tctcccgaac
     3961 tcgttttccc tgccgacttt accttgcgcg tcgccctgaa aaaagaagaa aaagtcctgc
     4021 ctgccggcgt catccgcgat attttggaag agaaggtggc ggaaatccaa aacaatgaag
     4081 cccgcaatgt cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca gacgacctgc
     4141 tgccccgagc gtttacccgc agcagccgta cagaagcggt gtttaacacc cgccacggct
     4201 acctgctcgt caataacgcg gcttccgcca aagcagaaaa catcctgacc aagctgcgcg
     4261 aagctttggg cggtttggaa gcctcgctgc cgaataccaa acaatcgccc tcttctctga
     4321 tgaccggctg gctgttgcaa gggcattgcg aaggcggttt tgaattggac agcgattgcg
     4381 aactcaaagg tacgggcgat attgttcccg tcgtcaaagt atccaaacaa gatttaaccg
     4441 ccgacgaagt ggttcaacac gtcaaaaacg gtaaaaccgt aacccagctc ggtttggttt
     4501 ggcgcgaaca aatcgccttt atcctcactc aagacttcac actcaagcgc atccaatacc
     4561 tcgacgtatt gcaggaagaa gccgaaagca acggcgacga tgccgccagc cttgccttcg
     4621 cctcgcaaat cctgatggct gaatccgtca gcaccatgtt ggaagagctg gtttcctatt
     4681 tgggcggctg gcaagattga tttttcagac ggcttttata ccgtcagcca aaatccaatg
     4741 gaataaccct tatgcttaaa catctcgcat tcctactgcc cgccatgatg ttcgccctcc
     4801 ccgccgcgtc cgccgttctg acttcctatc aagaacccgg ctgcacctac gacggcggag
     4861 tcggaaaaga caggcttcct tcaggcaaag gcatatggcg ttgccgggac gggcgcggtt
     4921 ataccggttc gtttaaaaat ggcaaattcg acggacaagg cgtttatacc gttgccgcca
     4981 accgcgaaat atttatcgaa ccgttcaatt ccgacagtac caaattccgc aacatggtac
     5041 tctcgggcac gttcaaaaaa ggcttggcac acggcagatt taccgtctcg caaaacggcg
     5101 aaaccctctt cattatgaaa tgcgaaaacg gcatgattaa agaagtgaag ctgcccaaaa
     5161 acaaataagc cccgttttca gacggcatcc cccaatgctt tgtccgaaca ctgcaagaaa
     5221 caaggaaaac attatgagca tcaagtccga caaatggata cgccgcatga gcgaagaatt
     5281 cggcatgatc gaccctttcg agccgaacca aatcaaagaa gccgacggca aacgcatcat
     5341 ctcctacggt acgtccagct acggctacga catccgctgc gccaacgaat ttaaaatctt
     5401 caccaacatc aacagcacca tcgtcgatcc caaaaacttc gacccaaaaa acttcgttac
     5461 cgttgaagac gactgctgca tcatcccgcc taattccttc gcactggcgc gcacggtcga
     5521 atatttccgc atcccgcgca acgtcctgac cgtctgcttg ggcaaatcca cctacgcccg
     5581 ctgcggcatc atcgtcaacg ttaccccgtt cgaaccggaa tgggaaggct acgttaccct
     5641 cgaattttcc aacaccaccc ccctgcccgc caaaatctac gccggcgaag gcgtggcgca
     5701 agtcctcttc ttcgagagcg acgaaatctg cgaaacctca tacaaagacc gcaacggcaa
     5761 atacatggga caaaccggcg taaccctgcc caaagcctga aatataaatg ccgtctgaac
     5821 ccttatcgtc gggtttcaga cggcatttgc cgttcacctc aaatcagaac tgaaccgtcc
     5881 cattgacgct gatgctgatt tcctccacac ccggcgcggc ggaatccgta ccctccacgc
     5941 cgtaacttgc cgccatcggc atggcacgaa gcatttttgc ctgagcagct ccttcacccg
     6001 cgatatggct gccgatgtgt cccaaattta atttgacgat tttataaccg gacgtaccca
     6061 atatgcccga caacttgtct gcacgcgcct tgaaacgcaa aatagcatct ttactgacct
     6121 gatcgatgac ctcgtttcgg cgttcgcgcg acacatggaa atccgtatat tccaatgcgg
     6181 catctgcctg aacatcggca ataaaacggt ttaactcatc aaaatccctc ccttcgacct
     6241 taaattccgc acgctcctcc caacctgttt gaatgcgtct gccgttggta tattgatagc
     6301 gcggcatcgc actgcgcgat accaattcgg ttttaaagct accatttttc gattttctga
     6361 tgaacttgtt gaatttttta acaaactcgg cattgacggc atttttgtcc cgtccttccg
     6421 ccgccacctg taaacgcgcg gacattgtat cctgaaccgc ctcgacaccg gccgattcgg
     6481 aaaattcgac aatattgtaa ttcaatgctt cagccgctgc cggaaaagat accgccaata
     6541 gggaagccgc caaaatagaa cgcagcatat ccgtatcctt acttataaaa ccgccaatct
     6601 agcatatcgg cgcgctaaat tgcgttagcg taaggcaaca atttaaatat tcggtctgcg
     6661 tctgtcttat aataaccgcc tttccgatac ggaatcccac tatgcccaaa cccttgcacg
     6721 tcctcgtcat cccctcatgg tatccgcaat ccgaacagga tgtggacggg atttttttca
     6781 aaatcaggca ctggcattgc agaggaaagg catcaaaacc gccgtgcttg caccgatgtt
     6841 ccgctacttg cggaaagaaa cagcaagcat cctgaccgac cttacggttt tgcaaaatat
     6901 cggcaaagcg gtttggacat ctatgcatgg cacggcatgt atttcttccc ccgctttccg
     6961 ttcatcgaca tcgaccgcat ccgctgggtg cgcgtgggtt tgaaagcctt caaacactac
     7021 atccgcgaaa acgggctgcc cgacctgatt cacgcccacc gcatgaacta tgccggcata
     7081 ctcgcccaaa aaatttccca aaaatacggc atcccctatg tcgtcacgaa acacagcagc
     7141 accattacgc gcggtctgat ccgtccccac caatggcagc ctatgaaaaa tgcggcggcg
     7201 cacgccgccg cacgtctcgc catcagccgc catttcgcac acgtcctgca acacaaatac
     7261 ggctgcgaat ggcagtacct ccccaacata ctgggcggaa tattcaaccg accgtttgaa
     7321 caaagagaaa aaaacaacaa accgcatttc gtgttctgca ccgtctcgca ccttcgccgt
     7381 ctcaaaggac acgatgtcct gctcactgcc tttgcccggg cgttggcaca atgcccgcaa
     7441 ctgcgtctaa acatcggcgg cagcggacag gaagaacagc ggctgaaaca gcaggcggca
     7501 gacttgggca ttacccatgc cgttacattt ttgggcgcac tgcaacccga agcagtcttg
     7561 gatttgatga agaacagcga cgcatttgtc cttgccagcc gcaccgaaac cttcggcgtg
     7621 gtctatatcg aagcactgtc ccaaggattg cccgtcattg caacacgctg cggcggtgcg
     7681 gaatctattg tttcagacgg caacggatat ttggttcctg ttgacgacga cgatgccctt
     7741 gccgacgcac tcatcaaaat gtatgaacac cactctgatt tcgaacctgc ccgacttagg
     7801 gaaaactgtc tgaacgaatt tggcgaaaat gccgttatag gcaggttgat cggcattttc
     7861 cgacaggcaa tcgcagaata cggtaagaaa atacc
//
LOCUS       DAWHXS010000089         7846 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWHXS010000089 DAWHXS010000000
VERSION     DAWHXS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.089"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            28..1143
                     /gene="asd"
                     /locus_tag="WIE22_001604"
     CDS             28..1143
                     /gene="asd"
                     /locus_tag="WIE22_001604"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ4127662.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPISEIEAILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            complement(1236..1766)
                     /locus_tag="WIE22_001605"
     CDS             complement(1236..1766)
                     /locus_tag="WIE22_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ4127663.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(1776..2120)
                     /locus_tag="WIE22_001606"
     CDS             complement(1776..2120)
                     /locus_tag="WIE22_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127664.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            complement(2107..2886)
                     /gene="xth"
                     /locus_tag="WIE22_001607"
     CDS             complement(2107..2886)
                     /gene="xth"
                     /locus_tag="WIE22_001607"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ4127665.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIATSGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            complement(2969..4390)
                     /locus_tag="WIE22_001608"
     CDS             complement(2969..4390)
                     /locus_tag="WIE22_001608"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ4127666.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGRGRPGWHIECSAMSENLFG
                     NTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNNAQL
                     AGRLKALGGIIGLLQRDPTEFLQGGVASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            complement(5078..6127)
                     /locus_tag="WIE22_001609"
     CDS             complement(5078..6127)
                     /locus_tag="WIE22_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ4127667.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            complement(6210..7364)
                     /gene="obgE"
                     /locus_tag="WIE22_001610"
     CDS             complement(6210..7364)
                     /gene="obgE"
                     /locus_tag="WIE22_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ4127668.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDSPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEQAAAKAEIAEQQPKTDTGVFKPE"
     gene            complement(7656..7846)
                     /locus_tag="WIE22_001611"
                     /pseudo
     CDS             complement(7656..7846)
                     /locus_tag="WIE22_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1815 a         2013 c         1935 g         2083 t
ORIGIN      
        1 acgatttttt cctcaaggag caacacaatg aaagtaggtt tcgtcggctg gcgcggtatg
       61 gtcggttcgg ttttgatgca gcgtatgaaa gaagaaaacg acttcgccca cattcctgaa
      121 gcgtttttct ttaccacttc caacgtcggc ggcgcagccc ctgatttcgg tcaggcggct
      181 aaaacattat tagatgccaa caatgttgcc gaactcgcca aaatggacat catcgttacc
      241 tgccaaggcg gcgattacac caaatccgtc ttccaagccc tgcgcgacag cggctggaac
      301 ggctactggg ttgacgcggc atcttctctg cgcatgaaag acgacgcgat tatcgtcctc
      361 gaccctgtca accgcaacgt tatcgacaac ggcctcaaaa acggcgtgaa aaactacatc
      421 ggcggcaact gcaccgtctc cctgatgctg atggctttgg gcggcctgtt ccaaaacgat
      481 ttggtcgaat gggcaaccag catgacctac caagccgctt ccggcgcagg tgcgaaaaac
      541 atgcgcgaac tcatcagcgg tatgggcgcg gttcacgccc aagtggcgga cgaacttgcc
      601 gatcctgccg gctcgattct cgacatcgac cgcaaagtat ccgatttcct gcgcagcgaa
      661 gactatccga aagccaactt cggcgtaccg ctcgccggca gcctgattcc gtggattgac
      721 gtggatttgg gcaacggcca gtccaaagaa gaatggaaag gcggcgtgga aaccaacaaa
      781 atcctcggcc gcagcgacaa tccaaccgtg attgacggcc tgtgcgtccg catcggctct
      841 atgcgctgcc acagccaagc ccttaccctg aagttgaaaa aagacctgcc catttccgaa
      901 atcgaagcga ttttggcagg cgcgaacgac tgggtgaaag tcatccccaa cgaaaaagaa
      961 gccagcatcc gcgagctgac ccccgccaaa gttaccggca cgctgtccgt ccccgtcgga
     1021 cgcatccgca aactgggtat gggcggcgaa tacatcagcg cgttcaccgt cggcgaccag
     1081 ctcttgtggg gcgcggccga gccgctgcgc cgcgtattgc gtatcgtgtt gggcagcctg
     1141 taagccctgt ttgaatggaa atgccgtctg aagcctgttt cagacggcat tttccttgca
     1201 accccagtta accgaaattc cgataaggca agcgttcacg ccagcaacat ttcctgcatc
     1261 agcttcatac cccactgcca gccgccgagc atgccgttca aactgcccga atgcggggaa
     1321 accaacaggc gggcgttcca caaatccgcc tgtttttgcg cccaaccgtg cggcacgccg
     1381 ccgtgttcgg gtacaaccaa tgcggcacgg cagggacagc ggacgcgttg gaaagtgtgt
     1441 tccgcatcgt cgggaaaaat atcgggacgc tgcggtacaa ggatgatgtt ggcaattttc
     1501 ttccgtgtca ggatgtctgc ctgatacagc cacgccaaaa atgcggccgc gcccgcaccg
     1561 tgtgcgacaa cggcgacgta tttgccgcgt atgcgttcaa atgccgtctg aagccctgcc
     1621 tgccattccc ctatgctttg accggccgac gcttcggaca tctgcacgac gggataactg
     1681 atcgcccagc ggtctatcca catttccgct tcatccgcat cgcgtaccag ccacagcgtc
     1741 aaatcctcaa gctcgaaact ttccattgtc cgcgcctatt tgagcaggtc ttgcagcgtg
     1801 aacgcaatca gcagcgcggc aggcacactc aacagagcgc acacggcgag gcaggcaaga
     1861 acgtttgcca acagccgcca gcctttccaa cccaaacatt tggacgcaat cagcaggcag
     1921 ggcaggcaga tcagcagcca caccaacgcc catgtcgggt ttgccttggt cggcgcaagc
     1981 cagccttgca tccgcgacaa cataaatatc gcccacacca atatgggcag gataaacgca
     2041 gcgacaaccc atgctgcgcc tattcctgtt tttccgtcca cattccaatc atatttaccc
     2101 aaaactttat tcggcagcat agtcatactc catgaccagc ggcgcatggt cggaaaattt
     2161 ttcatcttta taaacgtgtg cggacacggc tttggcggca agttcgggcg taaccatctg
     2221 ataatcgatg cgccacccga catctttcgc atacgcctgc ccgcggttgc tccaccacgt
     2281 gtagcccggc acatcgggat aaagcgtgcg ccacatatcc gtccaaccga gcttgtggat
     2341 aaccttgcct atccactcgc gttcttccgg taggaagccc gaatttttct gattgccttt
     2401 ccagtttttc aggtcgatgt tttggtgggc gatgttccag tcgccgcaaa cgacaatgtc
     2461 gcgtccttcg tttttcatcg cttcgagcat agggtaaaac gcatcaagga aacggtattt
     2521 cacctgctgg cgttcttccg cgctgctgcc gctgggcaag taaagcgaaa taacgctcaa
     2581 cctgtcgaaa tcgcaacgca caaaccgccc ttccctgtcg aattcttcaa tgcccatacc
     2641 gatttgcaca ttgtcgggtt tgcgtttgct gtacaccgcc acgccgctgt aaccgcgctt
     2701 ctcggcgcaa tgccaatggc cgtgcatccc gtgcggattt ttcatatcgg cagacaaatc
     2761 agcctcctgc gctttgagtt cctgcacgca gacaatgtcc gcgcccgatg tggcgatgta
     2821 ttcgtaaaaa ccttttttgt aggcggagcg gatgccgttg acgttggcgg aaatgatttt
     2881 aagcataata aaaataagtt ctcacaataa aaatgccgtc tgaacaaaaa agggcaaaat
     2941 gcggcacatt tacccttttc gatggatttt aaccgcgccg ccaagtcgtg ccgccggcgt
     3001 tgtcttccaa aatgattttg tgttcgttca gaaggtcgcg gatgcggtcg gattccgccc
     3061 agtttttatc ggagcgcgcc tgtttgcggc gggcgatcaa gtcttcgatt tcttcgttgg
     3121 agagaccgtc tgaagccacg ccgccttgca ggaactcggt cggatcgcgt tgcagcagtc
     3181 cgatgatgcc gcccaaagcc ttcagacggc ctgccagttg cgcgttattg gttttgttca
     3241 cttcgccggc aagttcgaac aacaccgcca ccgctttcac cgtatcaaaa tcatcattca
     3301 tcgcaacata aaagcggcgc gtgtagtcat cgccggcttc agacggcatc ggatcggcgg
     3361 gcggcgtatt tttcaaagtc gtatacaaac gcgtcaacgc gccttttgcg tcgtccaaat
     3421 gcgcgtcgga atagttcaac gggctgcggt agtgggcgcg caggatgaag aagcgcacga
     3481 cttcggggtc gtattgtttc aacacttcgc ggatggtgaa gaagttgccc agtgatttgg
     3541 acattttttc gccgtccacg cggataaagc cgttgtgcag ccaatatttg acgtggctgg
     3601 cgatgctttg cccgtggtgg gtttgcgcgt gatgatgacc gcaggtatgc cccgtcgcgc
     3661 cgacgctttg ggcaatttcg ttttcgtggt gcggaaactg caaatccgcg ccgccgccgt
     3721 ggatgtcgaa ggtattgccg aacaggtttt cactcatggc ggagcattca atgtgccagc
     3781 ccggccgtcc cctgccccaa gggctttccc acgccggttc gcctgctttc gccgctttcc
     3841 acaacacaaa atcaagcgga tcgcgtttga aaccgtccac ttccacacgc tcacctgcac
     3901 gcaggtcgtc taacgatttg cccgataatt ggccgtaagc ggcaaactcg cgcacggcgt
     3961 agtaaacgtc gccgttggcg gcaggataag ccttgccgtt ttgaatcagg gtttcaatca
     4021 tcgcaatcat ttgcggaatg ttttccgtcg ccttcggctc gatatcggga cgcaacacgc
     4081 ccaaagcatc ggcatcttcg tgcatagcct gaatgaaacg cgcagtcagc tcgccgatgg
     4141 tctcgccgtt ttcagccgca cgggcaatga ttttgtcgtc gatgtcggtg atgttgcgta
     4201 cataagtgag cggatagccg cactcgcgca accaacgggc aatcatgtca aacaccacca
     4261 tcacacgggc atggcctaag tggcaatagt cgtaaacagt catgccgcaa acgtacatac
     4321 gcacgttttc agggtcgatg ggggcaaagg gttctttttg gcgggtaagg gtgttgtaga
     4381 tggtggtcat gggactatgg attaatcttt gttgctcgga tgataatttc tgttctgttc
     4441 ctgtagatac ggaccaagga acattacgta gttgcggatt attaatatgg ctgatatttg
     4501 tgaaaattgg ttctgcataa cagtttgcaa aattttttgt aaattctgat aatttaaact
     4561 tatcttttaa taagtttgct aaatctgatg acgagggata aagtttactt cttatactag
     4621 gcatttcaat atgaaggact atttttattt cgttacaatc taaagccaag cgagaaaaat
     4681 ctttttcttc ctgtttttct gctttaaatt tagcagaaac caatcctgcc aatgaatctc
     4741 gaatttttct tgcgattaaa tatggtaggt cagaaagttt ttcatctatt gattgggcat
     4801 ttggctcaag cccaagtcgg taataatctt taatttcgat tagccaaagt gtcgattcat
     4861 gaagggctat tatatctaca cctgagctgc cattatcgtc atctacactt tgatttatcc
     4921 cgttctttcc cttttcattt gtatcaattt tattacgtaa attacaactg ttctgaaaaa
     4981 ttttataatg ttcccattcg tcatacttgg taacgtaata atcttcagga aaagcaaagg
     5041 ttaatctctt ttctgtgatt gtagtcatag cttaacctca aatattcaga tacctgtctg
     5101 cctgcataat gttttcatct aacaatatca atgtgttcaa atcattaata ctgttccctt
     5161 gctccacttt tgttccatca tcggaagcaa tcaacgagaa aaaacgtaca ggtaaatccg
     5221 tgttattttc aagcttcaaa agttccaatt ctctcaataa gaataaagag tgtgttgcaa
     5281 taaaaacctg aataccctgt tgagataaag accaaataat acgggcagcc actttgatca
     5341 atttaggatt cagattagct tccggttcat cccaaaatag atagccttta tccagcaatg
     5401 cccctgttgc gattaaccgg gcaatcatga caaatttccg caaaccctct gctaccaaag
     5461 gtgcttcaat cttaccgccc gtatttgtca gcgatagata aaaccttcct tgttcttcag
     5521 atacttttcc gcccatcgcg ttctcaatag gttcgagcaa ttctcgaatt tttgtttctc
     5581 tggggccttt ggcaagcggg tgatttaatt gcatacaggc atcaaaccaa gtttcttcga
     5641 aagggatgct ttggttttga tacaaagaag tgaaccaagg gcaaagtgta attaattcgc
     5701 ggctgggtaa gaagataggt gtcggagtat attcattttc tttcaatccg atgctttgaa
     5761 cattgacttg cgatgatgag ttactggaaa aattcagact actatgcgta gtgccgtttt
     5821 gcagttttaa aacgatttcc gtacgcccgc gcccctgcaa acgtttgctc aacctgccca
     5881 aggaatcggg acggaaaaca ttcagtaatt tatcggcaaa acttttttgc aattctgttt
     5941 tcagtaatct gtttttggtg ttagatgtta cttctagcag gctgtataaa atttttaaca
     6001 aatgtgtttt gccacaaccg ttttcggcaa caataacatt gagattttca gaaaattcaa
     6061 aagtatcgtt tggaagaacg gtaaagtttg tcaactcaag cgactggata tattggttag
     6121 atgacatttt taatccattt caatcttgct ttaaaattgt ttcaaacaac cttttgtaga
     6181 acaaatatcg tctgaaaccc tttctttttt cactccggct taaacacgcc cgtatccgtt
     6241 ttcggctgct gctcggcaat ttcggctttt gccgctgcct gttccgcttc tgctttttcg
     6301 gcttcgatac gttttttctc ggtcaggtat tggttgattt ggtgtaccaa ttcctgcgtg
     6361 ccttggtggg tcagcgcgct gatttggaag aggcgcgggg tttccatgtc aaattggaaa
     6421 cggtcgtcgg gtttggggga gtcccagccg acggcttcga ggaaggcggc agtgcgcgtt
     6481 tgggcttctt cttcgtcaag catgtcgagt ttattcagca ccagccagcg cggtttgccg
     6541 taaagttctt catcgtattt gcgcaattcg ttgatgatgg caagtgcttc ttcggccggg
     6601 ttgacggttt cgtcgaaggg cgccaaatcg acgacgtgca gcaataatcc ggtgcgcgat
     6661 aagtgtttga ggaaacgatg ccccaaaccc gcgccttctg ccgcgccttc aatcaggccg
     6721 gggatgtcgg ccatcacgaa gctgtggttt tcgtcgatgc gtaccacgcc taagtttgga
     6781 tgcagggtgg tgaaggggta gttggcgatt ttggggcgtg cggcggatac ggcggtaatc
     6841 agggtggatt tgccggcgtt gggcataccc aataagccga catcggcgag gactttgagt
     6901 tcgagttgca gggaacgggc ttcgccttct tcgccgggcg tggattgttt tggggcgcgg
     6961 ttgacggagg atttgaagtg gatgttgccc aagccgcctt tgccgccttt ggcaaggcag
     7021 acgcgctgtc cgtgataagt gaggtcggca acgttttcgc cggtgtcgag gtcgcggata
     7081 agggtgccga cgggcatttt gaggacgatg tcgtccgcac ctgcgccgta acggtcggaa
     7141 ccgtggcctt tttcgccgtt tttggcttgg tagcgtttaa cgaagcggta ttcgacgagg
     7201 gtgttggtgt tttcgtcggc ttctgcccaa acgctgccgc ctttgccgcc gtcgccgccg
     7261 tccggaccgc cgcgcggtac gaatttttcg cggcggaaac tggttgcgcc attaccgcct
     7321 ttgcctgcgg cgacttcgat ttttgcttcg tcgatgaatt tcattcaatg ctcttgtttg
     7381 ttggtttcaa atgggggttc agacggatta ccgtgtgttt tgatgccgtc tgaacagaat
     7441 ttcggacgct attataaggg ataagcggta tttcaacacg ccgtacccaa actatttgtt
     7501 ccgcccatct taatgaattt ttaagcaaat cttcagcctg caaacaaaat tatgtccaac
     7561 ttctttggta caatcgcgcc tttttgacat tccgacccga cggaatgtcc gttcaaaccg
     7621 ttacatataa taagttttgg ggctgtccta gataactagg ataaactcgg ttttactaat
     7681 tgttttaaaa tggaacaaga acttttatct cactgttgtt aaaacgccat tcgcactcct
     7741 ttaaatacag ctcaaaatgc gctttgggaa tgccgttaaa cttgcgtaaa tgacgttttg
     7801 cctggttcca aaagttctca attccattaa tatggttttg tcgttc
//
LOCUS       DAWHXS010000090         7705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWHXS010000090 DAWHXS010000000
VERSION     DAWHXS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.090"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            201..1736
                     /locus_tag="WIE22_001612"
     CDS             201..1736
                     /locus_tag="WIE22_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ4127669.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1733..1825
                     /locus_tag="WIE22_001613"
     CDS             1733..1825
                     /locus_tag="WIE22_001613"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ4127670.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1861..3105)
                     /gene="lysA"
                     /locus_tag="WIE22_001614"
     CDS             complement(1861..3105)
                     /gene="lysA"
                     /locus_tag="WIE22_001614"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ4127671.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPVLYDAYHHIEAVETKNISPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3116..3286)
                     /locus_tag="WIE22_001615"
     CDS             complement(3116..3286)
                     /locus_tag="WIE22_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ4127672.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3357..3680
                     /gene="cyaY"
                     /locus_tag="WIE22_001616"
     CDS             3357..3680
                     /gene="cyaY"
                     /locus_tag="WIE22_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ4127673.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3711..4130
                     /locus_tag="WIE22_001617"
     CDS             3711..4130
                     /locus_tag="WIE22_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ4127674.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            4152..4658
                     /gene="luxS"
                     /locus_tag="WIE22_001618"
     CDS             4152..4658
                     /gene="luxS"
                     /locus_tag="WIE22_001618"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ4127675.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            4804..7620
                     /gene="polA"
                     /locus_tag="WIE22_001619"
     CDS             4804..7620
                     /gene="polA"
                     /locus_tag="WIE22_001619"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ4127676.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPWDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIHNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VGGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         1787 a         2068 c         2082 g         1768 t
ORIGIN      
        1 acagccccta aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt
       61 ttacgataag cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga
      121 cattatccct gtcagacgct atttctatat atttcgccta taatggcttg tttttaataa
      181 ataattcaag aggtatcaac gtgtctgatt ccaagacgaa agaacgcgcc acattcggca
      241 cgcgccgcgc gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc
      301 gtttccccta tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg
      361 cgcttctgac ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc
      421 gtggttctgc gcccttggct ttccgccgcc tcggacgatg gtttgagccg gtcggctggt
      481 ggaacgtgat gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg
      541 caagctatac ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct
      601 ttaaggactt cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag
      661 tcgccggtcc tttggcgggc gtgtgggttt ttaccgccgc cattatggcg ttgggcgtgc
      721 aaaagggcgt ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga
      781 ttatggtcgg catttcacta accctgccgg gtgcggcaaa gggcttggac gcattgttta
      841 cgcccgactg gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt
      901 tcttttcgct ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa
      961 aaaccgactt gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc
     1021 tcgcgggcat cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg
     1081 tcaacgaggt tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca
     1141 accaggcacc gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg
     1201 gcgttacgtc gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga
     1261 acatcgggcg cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc
     1321 tgctgttcgg tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca
     1381 cctacggcat tgttgccgcc ggctttgttt atgttgccgc catcatcatc agcggcaggc
     1441 tgccggaatt acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga
     1501 cggtctgcgt cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg
     1561 gcctgatgga gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg
     1621 ggatgtcggc ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg
     1681 gtcaggattt caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac
     1741 ttccgccatt gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc
     1801 cctgttaagg ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaaccgct
     1861 tcagacggca ttttgatgtt cggcttgcag gcaggcgagt tcgtttgcca tttgctgttc
     1921 taaggtttcg cgtcggcgga tgagtttgta tccgccaccg ttaaccaaca cttccgccgc
     1981 acggttgcgc gcgttgtaat tgctcgccat actggccccg tatgcgcccg cgctgcggat
     2041 aagcagcaaa tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt
     2101 ttcacaaatc ggaccgacga tgttggcggt cagcggcgag atgtttttgg tttcaaccgc
     2161 ttcgatgtgg tggtaggcat catataggac ggggcgcatc aaatcgttca tcgccgcatc
     2221 gaccatcaca aagtttttct cttcaccgtg tttgacgaat tcgacacgcg tcaacaacgc
     2281 gcctgcgttg ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc
     2341 gatcagtttt tgaaccgctt gggcatacgc gcccaaatca ggcacttttt cgttttggta
     2401 aacaatgccg acgccgccgc ctaagtccaa atgttccaaa acaatgcctt cggcggcaag
     2461 cgcgtcaacc aaaatcaaaa tgcgttcgca ggcttcgacc agcgggctta agtcggttag
     2521 ttgggagccg atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg
     2581 gcggtaggct tcgagcgcgt cggcgtaggc gataccgaat ttgttggctt tcaaacctgt
     2641 ggagatgtaa ggatgggttt tcgcatcgac atcgggattg acgcgcaggg agacgggcgc
     2701 ggttttaccc aaacgcgcgg caactttctg aatacggtcg atttcgggga tgctttccat
     2761 attgaagcat ttcacgcctg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac
     2821 gcctgaaaat atggtttttg ccgcgtcgcc gcctgccgcc aaaacgcgtg ccaattcgcc
     2881 gccggacaca atgtcaaaac cgctccccaa agaagcaaag tgtttgatga tgctcaggtt
     2941 gccattggct ttaaccgcat agcagacgag cggggagagc tcggcaaagg cagtttggta
     3001 gtgttcaaat gcttcggtca gcgcggattg gctgtacaca taaagcggtg tgccgaatgc
     3061 ttcggcaagg cgggggtagg ggacttgttc gcaaaatagg gtcatatttt cgttttcatt
     3121 tttgggttgg aggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattacgc
     3181 cgaaacgcgc cttgtcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggccg
     3241 agagcaggag ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt
     3301 cataagcgcg aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga
     3361 tgaccgaaag cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa
     3421 tcgacgaaaa cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag
     3481 ccggagacgg cacgcaaatc atcgtcaacc gccacacgcc caaccaagaa ttgtggattg
     3541 ccgcaaaaag cggcggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg
     3601 acagccgcga tttttatgat gttttgaacg aagcactgag cgcggcttcg ggcgaagcgg
     3661 ttgagattga ggaattgtga ttcaatttga tataaaaaga ttgttaccca atggcacaat
     3721 tacctctata tcggactgcc gaaatcggaa attttactgt cggcacgcct aaagttttag
     3781 aatcattttc caaacatatc ccttatggtg tcgtctttga agacgacggc gacacgggct
     3841 acttctatgc tgcttcgcaa gacgggattt tagatgcctt gcacatctac aatgtcgaag
     3901 atgtatccga caaacatatc cccaatcatg tcttgatttt atgggatgat gcctgcacca
     3961 tagccgcatt gtgtgtcaac gactacattc atgccgtcta tgattttgtc gaacaggcag
     4021 gatattgccg caacggcttc cctgaagcag gcggcgaatg ggtgaaagtc gaaaaccgcg
     4081 tcttggatga tgaattgctg gacaaaatcc tatcccgaga acctacataa ccctcacaaa
     4141 aggataccca aatgccccta ctagacagtt tcaaagtcga tcacacccgt atgcatgccc
     4201 ccgccgtacg cgtggcgaaa accatgacca cgcccaaagg cgacaccatt accgtgttcg
     4261 acctgcgctt ttgcattccc aacaaagaaa tcctgcctga aaaaggcatc cacacgctgg
     4321 agcatttgtt cgcaggtttt atgcgcgacc acttgaacgg aaacggcgtg gaaatcatcg
     4381 acatttcccc gatgggctgc cgcaccggtt tttatatgag ccttatcggc acgccttccg
     4441 aacagcaggt cgccgatgcg tggctggctt cgatgcagga tgttttgaat gtcaaagacc
     4501 aaagcaaaat ccccgagttg aacgaatacc aatgcggcac ttatcaaatg cactcgctcg
     4561 ccgaagcgca acaaatcgcg caaaacgtgt tggcgcgcaa agtggcggtg aacaaaaatg
     4621 aagagctgac gctggatgaa gggctgctga acgcctaatc cgccaaaaat gccgtctgaa
     4681 caagggtttc agacggcatt tgccttttcc gttataatcc ggggttgtgc gggggcgggt
     4741 tttaagccgg catcgtcctt ccctattttt ttctgtccct tatcggtttt aagcgggttt
     4801 tttatgtcca acagacctac actcctcctc gttgacggat cgtcctacct ctaccgcgcg
     4861 tatcatgcga tggggcaaaa cctgaccgcc cccgacggcg cgccgacggg cgcgctgtat
     4921 ggcgtattga atatgttgcg ccgtttgcgg tcggaatatc cgcacgatta ttgcgcggtg
     4981 gtttttgatg cgaaaggcaa aaatttccgc catcaaatgt ttgaagaata caaggcgacg
     5041 cgcccgccga tgcccgacga tttgcgcccg caggcggaag cactgccgga tttagtgcgc
     5101 ctgacaggct ggccggtatt ggtgattggg caggtggagg cggacgatgt gatcggcacg
     5161 ctggcgaaac agggggcgga acatggtttg cgagtcattg tttcgaccgg cgataaggac
     5221 atggcgcagt tggtggatga gcgcgttacg ctggtgaaca cgatgagcag cgaaacgctg
     5281 gacattgaag gcgtgaaggc aaaattcggc gtgcgccccg accaaatccg cgattatctc
     5341 gcgctgatgg gcgacaaggt ggacaacgtg ccgggcgtgg aaaaatgcgg cccgaaaacg
     5401 gcggtgaagt ggctggaagc ctacggctcg ttgcaaggcg tgatagaaca cgcttcggaa
     5461 atcaagggca aagtgggcga aaacctgcaa gccgcgctgc cccaactgcc gctgtcgtat
     5521 gatttggtca cgattaaaac cgatgtggac ttgcacgccg agctttcaga cggcatcgaa
     5581 agcctgcgcc gtactacgcc gaaatgggcg cagctggttg tcgatttcaa acgctggggc
     5641 ttccgcacct ggctgaaaga agcggaatca aacatgaata ccggctcgac cgatgatttg
     5701 ttcggcagcg acagcatcgg cgagcaggcg gctttgaatg cggaaatgcc gtttgaaaaa
     5761 caagccgaaa aagccaccgc ccccgaaaaa ctggattatc aagccgttac caccgaagcg
     5821 cagtttgccg ctttgttgga caaactgtcg cgggcggaca caatcggcat cgatacggaa
     5881 accacgtcat tagacgcgat gaacgcctcg ctggtcggca tcagcatcgc tttccaagca
     5941 ggcgaagcgg tttacatccc cgtaggacac agcctgaccg ccgcgcccga acagcttgat
     6001 ttgcaagacg tattaggccg tctgaaaccg catttggaaa accccgccct gaagaaaatc
     6061 gggcaaaacc tcaaatacga ccaacacgtt ttcgctaact acggcatcgc cctgaacggc
     6121 attgccggcg acgctatgct cgcttcctac atcatcgaaa gccatcttgg acacggcttg
     6181 gacgaattgt ccgaacgttg gctgggcttg gaaaccatta cctacgaatc attgtgcggt
     6241 aaaggcgcga agcaaatcag ttttgccgat gtcgccatcg ggcaggcgac cgaatacgcc
     6301 gcccaagacg ccgatttcgc cttgcgcctc gaagcgcacc tgcgcgcgca aatggacgcc
     6361 aaacagcttg aaatgtatga aaaaatggag ctgcccgtcg cgcaagtatt gtttgaaatg
     6421 gaacgcaacg gtgtgcaaat cgaccgcgcc gaactcgccc gccaaagcgc ggaactcggc
     6481 gccgagctga tgaagctcga acaacaagcc tacgccgccg caggccagcc gttcaacctc
     6541 aattcgccca aacagctgca agaaatcctg ttcgacaaaa tgggcatccc gacaaaaggg
     6601 ctgaaaaaaa ctgccaaagg cggcatttcc accaacgaag ccgtgctcga acagctcgcg
     6661 cccgactacc ccctgcccaa aatcatcctg caaaaccgca gcctggcgaa actcaaatcc
     6721 acctacaccg acaaactgcc cgaaatgatt tctccctggg ataaccgcgt gcataccacc
     6781 tacgcccaag ccgtcgccat tacaggacgc ttggcaagca acaaccccaa cctgcaaaac
     6841 atccccatcc gcaccgccga aggccgccgc gtacgtcgcg cctttaccgc accgcaaggc
     6901 agcgtcatcg tttccgccga ctattcccaa atcgagctgc gcatcatggc gcacctctct
     6961 ggcgacaaaa ccctcatcgc tgcgttccaa aacggcgaag acgtacaccg ccgcaccgcc
     7021 gccgaagtgt tcggcatcgc ccccgaaaac gtctcgtccg aacaacgccg ctacgccaaa
     7081 accatcaact tcggcctcat ttacggcatg ggccaatacg gcctcgccaa atcattgggc
     7141 atcgacaaca tctccgccaa aaactttatc gaccgctact tcgcccgcta tcccggcgtc
     7201 gccgaataca tgcagcgcac caaagaacaa gccgccgcac aaggctttgt cgaaaccctg
     7261 ttcggcagaa ggctctacct gcccgacatc cacaacaaaa acgccaacgc ccgcgccgga
     7321 gccgaacgcg ccgccatcaa cgcccccatg caaggcaccg cctccgacct catcaaacgc
     7381 gccatgatag acgtgtcccg ctggctttca gagtgcgaag cctccccgtg ggacgaactc
     7441 ttacaaagca aactgattat gcaggtgcat gacgaactgg tgctggaagt cgttgaaacc
     7501 gaactggatt ttgtcaaaga aaaactgccg cagattatgg cgaaagtggg cggcggatta
     7561 ttggatgtac cgctggtggc tgaggttggc gtaggggaga attgggaaga ggcacattga
     7621 ttgaaaggtg ttatatgcta tctttattta aataaaattt aatttttggt atattttttc
     7681 taaatgttcc tatagtatag tggat
//
LOCUS       DAWHXS010000091         7664 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWHXS010000091 DAWHXS010000000
VERSION     DAWHXS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.091"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(14..430)
                     /gene="dksA"
                     /locus_tag="WIE22_001620"
     CDS             complement(14..430)
                     /gene="dksA"
                     /locus_tag="WIE22_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ4127677.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(577..1368)
                     /locus_tag="WIE22_001621"
     CDS             complement(577..1368)
                     /locus_tag="WIE22_001621"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ4127678.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1417..1824)
                     /locus_tag="WIE22_001622"
     CDS             complement(1417..1824)
                     /locus_tag="WIE22_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ4127679.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1824..2510)
                     /locus_tag="WIE22_001623"
     CDS             complement(1824..2510)
                     /locus_tag="WIE22_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ4127680.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2630..3673
                     /locus_tag="WIE22_001624"
     CDS             2630..3673
                     /locus_tag="WIE22_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ4127681.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3836..5062
                     /locus_tag="WIE22_001625"
     CDS             3836..5062
                     /locus_tag="WIE22_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ4127682.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5348..7498
                     /gene="yccS"
                     /locus_tag="WIE22_001626"
     CDS             5348..7498
                     /gene="yccS"
                     /locus_tag="WIE22_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ4127683.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1880 a         2555 c         1675 g         1554 t
ORIGIN      
        1 ttgtttgccg ccatcagtcg gcaaactgtt ttttcatcct ctctcggcgt tcttgggact
       61 caacagataa agtggctgtc gggcgtgcca gcagccgctt cagaccgata ggctctcccg
      121 tatcggcaca gaatccataa tccccttcat caatattgcg gatggtcgcc tgtattttac
      181 tgagaagttt tcgttcccga tctcgggtac ggagttccaa tgcgtactct tcttcctgtg
      241 tggcacggtc ggcaggatcg ggggctgatt cgtgttcttg gagatgccct gtcgtagcgg
      301 aagcattttc gatgagttcg tcttgcattt ttaccagcaa ttcgcggaaa aaagccaaat
      361 ggtcgtcatt catataatcg tcttccggcc cgctccaatt caaaatatct tgttctgtca
      421 gctttgccat aatgtatatc ctctcggttc accgaaaaaa acaaagcacc acaaagtggc
      481 tttattcctt tgatttttat aaggcttatt gcctaaggcg tttttgaaaa cgcgcatcat
      541 aacacgtttt ggattggttt ttttattttc tttacattat tgatattgcc gttccatttc
      601 ctgcgaacgg cgcacacagg cacaaacgcc ctcacttatg gcttcggcga cacgatgccg
      661 cctgaaggct tccacggctt cgtgggttgt cccgcctttt gacgttacat ttttttgaag
      721 cttctcgaaa tcttcacccg tctgctcggc aagggtaacc gctcctttaa acgttgccag
      781 actgagcgcg cgtgcttctg ccatatcaaa cccttgtcgg atggcggcat tttgcaatgc
      841 gtccagcaga taaaacacat atgccggccc gctgccgctg ataccggtaa tgctgtgcat
      901 ttgcgcttca tcttccaacc aaacagtcaa accgactgat ttcatgattc gatcggcaat
      961 cctgcggtct gtttccgata cttccgcttc ggcatacata ccggatacgc ccagcccgat
     1021 ttttccgggt gtattcggca taacccggac aatgcggcgt gttcccccga ggtaacggct
     1081 gagcgtatcg accggcaatc cggctgcgac agaaagcacc aatgcgccgt tggtgcggat
     1141 atttttgcac gcggcttcca tatcctgcgg cttgacggca aggattaaaa catcgtcgga
     1201 atgaagctcc ggcaaggttt cagaagtttc gacccccaac tctttttgca aacgttcgcg
     1261 cttttccgca ccccggttgg caatatggat gcggtaaccg ccttgtttga ccaatccgcc
     1321 cgcaacggca gccgccatat tgccgccgcc gagaaaataa acattcatta tttctaccct
     1381 attcaaaatt acctgactaa attcattccc aattttttac catcggacgc gctcggacag
     1441 accgttgcgc cgcaacccct gcttttccaa gcagtcaaat cctccctgtt tgaacgtctc
     1501 aatcaaaggt ttgccgtcac gcgtacgcca atccagcccg caggcttcaa acttccctat
     1561 tttcctgtaa aagtaagatt gttgtgcgga ttcatattcg tttgccctga cggaacgatt
     1621 gccgtctgaa agcggtatct cgtagtcctc taaagacggc ggactctcgc cgccaatgtc
     1681 ccaaaaatcg atggcagccg gttttctcgg cttacaccag ccggacaggg acgaacactc
     1741 ataccaaccc gccaactggc agcctgcaac cgtaaatgca agcaacgcag ccattgccgt
     1801 atattttatt gcccgaattt ccatcagccc cttttcccga aaatcgcgct gccgatacgg
     1861 acgtgtgtcg caccgcactc aatggcggca ggcatatcgt ccgacatccc catagacagc
     1921 acgtctgcct taacgccagc cgtattgagg tcggcaagca gtttccgcat cgtttgaaat
     1981 tgcaccttca actccgtttc actgctgttg gctttggcaa cacacatcag tccgcgtacg
     2041 acgatattcg gcagcttcgc cacttccaca gcaagcgcga ctgcttcttc gggcgcgaca
     2101 ccgtgcttca ccgcctcgcc cgcaatattc acctcgatac acacctgcaa gggcggcatt
     2161 gaggaaggac gctgttcgct cagccggaca gcggttttca gacggcatac ggtatgcacc
     2221 caatgcgcgc gttcggcgac aaacttggtt ttgttggact gcacatcgcc gatgacgtgc
     2281 cacacgatgt cggtcaaatc cgccaactct tccgttttgc cgtaccactc ctgaatatag
     2341 ttctcgccga aatcacgctg tccggcggcg taaacttcgc ggatgccgtc tgaagggaaa
     2401 gtcttaccga cggcaatcag gctgacggaa tgcggcttcc ttcccgcctg cagaaccaat
     2461 tttccaatac ggtcggacac ctcccgataa cgttcttgca acaccgtcat agattatccc
     2521 ctaattaaaa atgattaaac agttgaaacc cctccagtca gggcggtaca atcaaggttg
     2581 ttagaaccat tccaaccaat cgaaacatta tactaaacag agccgcatta tgcagattac
     2641 cgacttactc gccttcggcg ctaaaaacaa agcatccgac cttcacctga gttcgggcat
     2701 atcccctatg attcgggttc acggcgatat gcggcgcatc aaccttcccg aaatgagcgc
     2761 ggaagaggtc ggtaatatgg taacttcggt gatgaacgac caccagcgga aaatctacca
     2821 gcaaaacttg gaagtcgact tctcgttcga actgcccaac gtcgcccgat tccgcgtcaa
     2881 cgccttcaac accggccgcg gtcccgccgc cgtattccgc accattccca gcaccgtctt
     2941 atcgctggaa gaattgaaag ccccgagcat tttccaaaaa atcgcagaat cgccgcgcgg
     3001 catggtattg gttaccggcc ctaccggttc gggcaaatcg accacgcttg ccgcgatgat
     3061 caactacatc aacgaaaccc agccggcaca catcctgacc atcgaagacc cgatcgaatt
     3121 cgtccaccaa agcaaaaaat ccctgattaa ccagcgcgag ctgcaccagc acaccctcag
     3181 cttcgccaac gcgctgcgtt ccgcattgcg cgaagacccc gacgttatcc ttgtcggcga
     3241 gatgcgcgac cccgaaacca tcggcttggc actgaccgcc gccgaaaccg gacacttggt
     3301 tttcggcacg ctgcacacga ccggcgcagc aaaaaccgtc gaccgtatcg tggacgtatt
     3361 cccggcggga gaaaaagaaa tggtgcgctc tatgctgtcc gaatcgctga ccgccgtcat
     3421 ctcccaaaac ctgctgaaaa cgcacgacgg caacggccgt gtcgcctcgc acgaaatcct
     3481 gattgccaac cccgccgtcc gcaacctcat ccgcgaaaac aaaatcacgc agattaactc
     3541 cgtcctgcaa accgggcagg cgagcggtat gcagacaatg gaccaatcgc tgcaatcgct
     3601 ggtgcgccaa gggctgatcg caccggaagt cgcacgcaga cgcgcgcaaa acagcgaaag
     3661 tatgagtttc tgacacacaa ccgccttccg gccataccgg cgggaaaaca aggcgcaaac
     3721 acgcgaggcg ggacgcagca tcccgcccgg ctacctttcc gaacaagaag cgtccgcctt
     3781 cctgttgaaa cctgccgccg caaactgcaa ggcttaaacc gaaaagaagc taacgatgaa
     3841 taccgataac ctgcacgaca tcttggacga aatggttcaa gtgtattctc aaaaaaaaca
     3901 aagccgatcc gaaaccccgg ccgaaatcgg cgcacacttc cacccgctgc tcgaccgcct
     3961 gtgcgaaacc gcagaagcac aaaacgcgtc cgacatcctt atcagcaaag gattcccgcc
     4021 ctcgttgaaa atcaacagcg cattaacccc gcagccgcaa aaggcgctga caggcgagga
     4081 aaccgccgcc atcgccgcat cgacgatgaa cgccgaacaa tcggaaatat tccggcgcga
     4141 cggcgaaatc aactactccg tccagtcgcg cagcggcacg cgctaccgcg ccaacgccta
     4201 ccacagccaa ggcagcgcag gtttggtttt gcggcgcatc aaccacgtca tcccgcaaat
     4261 gcaggaattg ggcctgcccg aaaaactcaa agacctcgcc gtcgcaccgc gcgggctgct
     4321 gattatcgtc gggcctaccg gttcgggcaa atccaccacg atggcgacta tgctcgaaca
     4381 ccgcaacaaa accctgccca gccatatcgt taccatcgaa gacccgattg agtttatcta
     4441 caaaccgcgc cgctgcatct ttacccagcg cgaaatcggc gtcgacacca taaactggca
     4501 gacggcggta caaaacgcta tgcgccaatc ccccgacgtg gtctgcatcg gcgaagtccg
     4561 cagcagggaa agtatggaat acgcgatgca gctcgcccaa accggccacc tgtgcatttt
     4621 tacgctccac gccaacaccg cgccgcagtc gctcgaacgc atactcaact tctaccccaa
     4681 agaacagcac aaccaaatac tgatcgacat cgccctcaac ctgaccggca tcatctgcca
     4741 acgcctcgcc ctcaaacaag acaaaacggg caggacggcg gttgtcgact tgctcatcaa
     4801 cacgcccgcc atccaagact tcatcctgaa gggcgacctg atgaacatca gtaaaatcat
     4861 ggaaaccgcc aaaaccgacg gaatgcagac gatggatcaa aaccttttcg aactgtaccg
     4921 tcacggcatc atcagttacg aagaagccct gcgccagtcc gtttccgcca acaacctgcg
     4981 attgcacatc caactgcaca aagaaggcaa aacgcccgaa ctcctttacg acagggtcaa
     5041 cggtctcaac ctcatttcct gatccgcaaa acccaatgcc gtctgaaaac cgcatccccg
     5101 ttttcagacg gcatgatttt atccgcccca ttcatgtgct acactttata gtggattaaa
     5161 tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag cacaaatagt
     5221 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt cagctaaggc
     5281 gaggcaacgc cgtactggtt tttgttaatc cactatattc acttcataac aataaaccgg
     5341 taaaaccatg aaaaccccac tcctcaagcc tctgctcatt acctcgcttc ccgttttcgc
     5401 cagtgttttt accgccgcct ccatcgtctg gcagctaggc gaacccaagc tcgccatgcc
     5461 cttcgtactc ggcatcatcg ccggcggcct tgtcgatttg gacaaccgcc tgaccggacg
     5521 gctgaaaaac atcatcgcca ccgtcgccct gtttaccctc tcctcgctca cggcacaaag
     5581 taccctcggc acagggctgc ccttcatcct cgccatgacc ctgatgacct tcggctttac
     5641 cattttaggc gcggtcgggc tgaaataccg caccttcgcc ttcggcgcac tcgccgtcgc
     5701 cacctacacc acacttacct acacccccga aacctactgg ctgaccaacc ccttcatgat
     5761 tttgtgcggc accgtactgt acagcaccgc catcatcctg ttccaaatca tcctgcccca
     5821 ccgccccgtc caagaaagcg tcgccaacgc ctacgacgca ctcggtggct acctcgaagc
     5881 caaagccgac ttcttcgacc ccgatgaggc agcctggata ggcaaccgcc acatcgacct
     5941 cgccatgagc aacaccggcg tcatcaccgc cttcaaccaa tgccgttccg ccctgtttta
     6001 ccgccttcgc ggcaaacacc gccacccgcg caccgccaaa atgctgcgct actacttcgc
     6061 cgcccaagac atacacgaac gtatcagctc cgcccacgtc gactaccaag agatgtccga
     6121 aaaattcaaa aacaccgaca tcatcttccg catccaccgc ctgctcgaaa tgcagggaca
     6181 agcctgccgc aataccgccc aagccctgcg cgcaagcaaa gactacgttt acagcaaacg
     6241 cctcggccgc gccatcgaag gctgccgcca atcgctgcgt ctcctttccg acggcaacga
     6301 cagccccgac atccgccacc tgcgccgtct tctcgacaac ctcggcagcg tcgaccaaca
     6361 attccgccaa ctccagcaca acggcctgca ggcagaaaac gaccgcatgg gcgacacccg
     6421 catcgccgcc ctcgaaaccg gcagcctcaa aaacacctgg caggcaatcc gtccgcagct
     6481 aaacctcgaa tcaggcgtat tccgccatgc cgtccgcctg tcccttgtcg ttgccgccgc
     6541 ctgcaccatc gtcgaagccc tcaacctcaa cctcggctac tggatactac tgaccgccct
     6601 tttcgtctgc caacccaact acaccgccac caaaagccgc gtccgccagc gcatcgccgg
     6661 caccgtactc ggcgtaatcg tcggctcgct cgtcccctac tttaccccct ccgtcgaaac
     6721 caagctttgg atcgtcatcg ccagtaccac cctctttttc atgacccgca cctacaaata
     6781 cagcttctcg acatttttca tcaccattca agccctgacc agcctctccc tcgcaggttt
     6841 ggacgtatac gccgccatgc ccgtgcgcat catcgacacc attatcggcg catcccttgc
     6901 ctgggcggcg gtcagctacc tatggccaga ctggaaatac ctcacgctcg aacgcaccgc
     6961 cgcccttgcc gtatgcagca acggtgccta tctcgaaaaa atcaccgaac gcctcaaaag
     7021 cggcgaaacc ggcgacgacg tcgaataccg cgccacccgc cgccgcgccc acgaacacac
     7081 cgccgccctc agcagcaccc tttccgacat gagcagcgaa cccgcaaaat tcgccgacag
     7141 cctgcaaccc ggctttaccc tgctcaaaac cggctacgcc ctgaccggct acatctccgc
     7201 cctcggcgca taccgcagcg aaatgcacga agaatgcagc cccgacttta ccgcacagtt
     7261 ccacctcgcc gccgaacaca ccgcccacat cttccaacac ctgcccgaaa ccgaacccga
     7321 cgactttcag acagcactgg atacactgcg cggcgaactc gacaccctcc gcacccacag
     7381 cagcggaaca caaagccaca tcctcctcca acagctccaa ctcatcgctc ggcagctcga
     7441 accctactac cgcgcctacc gacaaattcc gcacaggcag ccccaaaacg cagcctgaaa
     7501 aagtttcggc attttgtaag agagggcaga ttgtcagaca ggttgcaaga tagtggataa
     7561 agctttgcgc agggtaaatg cgtagcaact gaaccgtcat tcccacgaac ctacatcccg
     7621 tcattcccac gaaaacagaa aaccaaaaac agaaacctaa aatc
//
LOCUS       DAWHXS010000092         7495 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWHXS010000092 DAWHXS010000000
VERSION     DAWHXS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.092"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(184..939)
                     /locus_tag="WIE22_001627"
     CDS             complement(184..939)
                     /locus_tag="WIE22_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ4127684.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(1119..2278)
                     /gene="nirK"
                     /locus_tag="WIE22_001628"
                     /pseudo
     CDS             complement(1119..2278)
                     /gene="nirK"
                     /locus_tag="WIE22_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
     gene            2791..5046
                     /locus_tag="WIE22_001629"
     CDS             2791..5046
                     /locus_tag="WIE22_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ4127685.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTISAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            5225..5758
                     /locus_tag="WIE22_001630"
     CDS             5225..5758
                     /locus_tag="WIE22_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ4127686.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5821..7005)
                     /locus_tag="WIE22_001631"
     CDS             complement(5821..7005)
                     /locus_tag="WIE22_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ4127687.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(7045..7449)
                     /locus_tag="WIE22_001632"
     CDS             complement(7045..7449)
                     /locus_tag="WIE22_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ4127688.1"
                     /translation="MTAQPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1692 a         1796 c         2087 g         1920 t
ORIGIN      
        1 gaatgacgga aagttgcggg aatgacggaa agtggcggga atgacggttc gggcattcct
       61 taaattaccc gtgtatcgct gtaaatctta gagatggcgg aatatagcgg attaacaaaa
      121 accagtacgg caaggcgagg caacgccgta ctggtttttg ttaatccact ataattgaag
      181 gggttatcgg cttgtgcaac ggaagcccaa gttgtgcaag acatatttgg attgcaggct
      241 ggtacggatg ccgtagcgga ggaaggcggc atagttggac gagtcgctcg acccgataga
      301 cgcgccgctg caaaacattt gcgcgttggc attgccggaa gaaagcaggc tgctgttgaa
      361 atcttccgtc cattcccaaa tcagcccgtg catatcataa acgccccagt agttcgggcg
      421 gcctttgccg acatcgtgca ggcctttccg tccgccgtcg gcataccaat cgagaatagt
      481 gcggttgtag ccgggttcgt ttgagccgtt tttctgcgtg gcggaagcaa gtccggcaaa
      541 ttcccattcg tcaatggtcg gcaggcgttt gccttgtgcg gcgcaatagg cgttggcggc
      601 aaaccaggaa acattggtta ccggttgttt taattcgccc gccttcggcg catagctgcg
      661 gctgccgttt ttcatccaat gcttcaggta agcgggttct gcctgtttgg aaccgatcct
      721 gcctttttgc cattgggggt ggctgttgac aaattcggca aactcggcat tggtaacggg
      781 atatttatcc agtttgaacg gtttgacttt aatcaggccg gtatcttttt tcagataaag
      841 cgggcggtag ctgccgcctt cgatttgaac catttcggca gccgccgctt gagtgccggc
      901 gagtgccgca cagagaaaga gtaaccgggc aaacttcata tcatgcctcc tgacaggcag
      961 ttaaaattga tttttcagac ggcattttaa acaaaaagcc gcgaatgccg tctgaagaat
     1021 caatcttccg aaaggaaaga ttggttgtta aaaaaccact gccgtgcgta attaagtacg
     1081 gcggcagtgg ttcgtttgcc taaaaatggg tgttcaattt agtaatcgct ttttgcagga
     1141 gcagaggctg caggagcaga agctgcggga gcggaagcag caggagctgc accgttaccg
     1201 gcgtaagcgg tatcactcaa tttttgagtc atgatttcag ggttttctgc accttctact
     1261 ttcaattgac ccagtgcgcc tttgttgaat gcgcggaaga tagagtggtc aaccaaagtg
     1321 tagctgcccg ggatgtcgac tttgaattcg acgatggcag agccgccggc aggaacgatg
     1381 gtactttgta cgttttcgtt aatcagtttg ccgccttcaa cataaacttt gtcgaagatt
     1441 tcaccgatta cgtggaagga agacaccaag ttcgggccgc cgttaccaac gtacatacgt
     1501 acagtttcgc ctgctttggc tttcagggcg ttgtcgccgg cgatagcacc tacgtgaccg
     1561 ttgaatacga cgtattcagg ctgttcggca acagctttgt ccatatcgaa cggttgcagg
     1621 ccttgcgcac cttttttgcc tttggtgtag aagtcgcctt ggacgatgta gaactcttta
     1681 tccactttcg gcaggccttc tttaggctcg accaaaatca ggccgtacat accgttggcg
     1741 atgtgcatac cgaccggcgc gacggcgcag tggtagatgt acagacccgg ttgcagggct
     1801 ttgaagctga atgtggaagt gcggcccggg cggtaaaggt cgcggctgca ccgccgccct
     1861 gaccggttgc ggcgtggaag tcgacgttgt gcggaacggt agaagaagga ttgttggaaa
     1921 attccacttc aaccgtatcg ccttcgcgta cgcggatcat gcgacccgga acgtcgccgt
     1981 caaatgtcca gtagcggtat tccacaccgt cttccatggt catggttttt tcgacggttt
     2041 ccattttcac gcgtactttg gcagggtagt cgcggtcgat tgcaggaggt acttctggag
     2101 cgtgtgtggt aaccgcatcg ataacgggca gttcgcctgt cggcgtttcg gcggcggttt
     2161 gtgcggcgga acttgcggct tctgcggaag cggcaggggc ttgcgcggca ggttcgcctc
     2221 cgcaggcggc taatgcgaat aaggaagcaa tcattgcagc taaggcttgg cgtttcataa
     2281 tgttttcctt ttgtaagaaa agtagaggaa ttggttaaga acttggtgtg attatgcgtt
     2341 tgccccattg gtggcatttt gatttaagtc aaataattca taaaatatga acttaaaatg
     2401 atacttctaa tactttatag ctactaaaaa ttacaatgaa taggctgatt tgcttaaggg
     2461 cttcggcaaa tcaggcaaag gcagggtagt tgccgactaa agaataatga tagttattat
     2521 catatatgta tttgttttat atgtgagttt ctttgaacaa atgtttattt gtgcggtaaa
     2581 ccgtgctaca atctttaaca ttcatattct gtgaatttta aacagctgat ttatagtgga
     2641 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggaaccgat
     2701 tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg aggcaacgct
     2761 gtactggttt ttgttaatcc actatattaa atgggacagt acaagaagct gtggtacttg
     2821 ctgtttgccg ttctggcggt atgctttacc attcttggct atatgggcag cgaggtttat
     2881 aagaaagccc cgccttaccc cgaacaggtc gtttccgcat ccggcaaagt cttgatgacg
     2941 aaagacgata ttttggcagg tcagtctgca tggcagagta ccggcggtat ggaagtcggt
     3001 tcgattctgg gtcacggcgc atatcaggct ccggactgga cggccgactg gctgcatcgt
     3061 gagttgtccg cctggttgga tttgaccgcg caacagactt acggcaaaaa attcgatgaa
     3121 gtttcccctg aggaacaagc tgtcttgaaa acacgcttgg ctgacgaata ccgtaatcaa
     3181 agccgtgtga aagaagatgg ttctgtggtc atcagcgata cgcgtgtaaa agccatcgaa
     3241 agcatcctgc cttattatca cggtgtttac ggcgatgatc ccgcgctgca aaccacccgc
     3301 gaacactttg caatgaaaaa caacacattg ccaagccaag aagcgcgtga aaaactgttc
     3361 aacttcttct tctggacttc ttggtctgct tcgaccaacc gtcctgacga gaattttact
     3421 tacaccaaca actggccgca cgaacctttg atcaacaacg taccgaccac tgaaaactat
     3481 atgtggtcat tcaccagcgt tgttcttctg ctgatgggca tcggtttgct gatgtggggt
     3541 tattccttcc tgaccaaaca cgaagaagtg gaagtgccgt ctgaagaccc gatttccaaa
     3601 atccaactga ccccttcgca aaaagcattg ggcaaatacg tcttcctgac ggtcgccctg
     3661 tttgtggtac aggtattgct gggcggtctg accgcgcact acaccgtcga aggccagggt
     3721 ttctacggca ttgacgaggc gttgggcttt gaaatgtccg actggttccc ctacgccctg
     3781 acccgtactt ggcacatcca atccgccatc ttttggattg caaccggctt tttgacggca
     3841 ggcttgttcc tcgcgccgat tgtcaacggc ggcaaagatc ccaagttcca acgtgccggc
     3901 gtaaacttcc tctatatcgc cctcttcatc gtggtcggcg gttcttacgc gggcaacttc
     3961 ttcgcgttga cgcacatcct tcccccggaa tttaacttct ggttcggaca ccaaggttac
     4021 gaatacctcg atttgggacg tttctggcaa cttctgctga tggtcggtct gttgttgtgg
     4081 ctgttcctga tgctgcgttg cacgatttcc gcctttaaag aaaaaggcgt ggacaaaaac
     4141 ctgctggcaa tctttgtcgc ttctatggtc ggcgtgggcg tgttctacgc gccgggcctg
     4201 ttctacggtg aaaaatcccc gattgccgtt atggaatact ggcgttggtg ggtggttcac
     4261 ctgtgggtgg aaggcttctt tgaagtattt gccactgccg cctttgcctt tgtcttctac
     4321 aatatgggct ttgtccgccg cagtaccgcc actgcctcca ctttggccgc tgccgccatc
     4381 tttatgttgg gcggcgttcc tggtacgctg caccacctgt acttctccgg ctctacctcc
     4441 gcctctatgg caatcggtgc ctgcttctcc gctttggaag tcgtgccgct ggtgttgctg
     4501 ggccgtgaag catacgaaca ctggtcttac caacacctgt ccgactgggc gaaacgcctg
     4561 cgttggccgc tgatgtgctt cgtggcagtc gccttttgga atatgatcgg tgccggcgta
     4621 ttcggcttcc tgatcaatcc gccgatttcc ctgttctata tccaaggttt gaacacttct
     4681 gccgttcacg cgcacgccgc cttgttcggt gtgtacggtt tcttggcatt gggcttcgta
     4741 ttgctggttg cccgttattt gaaaccgaac gcgcagtttg acgacaaatt gatgacttgg
     4801 ggtttctggc tgctcaacgg cggcttggtc ggtatgattg ccatcagcct gctgcctgtc
     4861 ggcgcgattc aggcatacgc ctctatcacg cacggcctgt ggtatgcgcg tagcgaagag
     4921 ttcctgcaaa tggaaatctt ggatactctg cgttgggttc gtactgctgc tgacttgatc
     4981 ttcatcggcg gtgcaatctg cgttgccatc caagccacta aaatcgtatt cggccgcgac
     5041 aaataagttt tgcggtatga aatgagcaac tgcacccgtt ttcgggtgca gttttttatt
     5101 gcggattggg acgggaatgg aaaaggatgt ggatttttta ttttaatcgg ctatacaatt
     5161 gctgctttca tgccgtctga agcacgtttc agatggcatc ggtttacatc agataaggat
     5221 aaaaatgggt atttacgatt ttcaaatgaa agatgcggaa ggcaatgcgg ttgatttgtc
     5281 aggctatcgc ggcaaggttc tgctgattgt caacacggca acgcgttgcg gtctgacccc
     5341 gcaatacgag gctttgcaga agctgtatgc acaatatacc gcagaaggct tggagatttt
     5401 ggattttccg tgcaaccagt tccgcgaaca ggctcccgaa agcagcggag aaattgccca
     5461 agtgtgtatg atgaagtttg gcacgaagtt taagattttc gacaaaatcg aagtcaatgg
     5521 agcaaatacc gcgcccttgt atgcctacct gaaatccgtc aaaccgcaag ataaaggcaa
     5581 tcatctgttt aaagatttcg tgttgaagct cgcagctttg ggagagaagc gcgacgaagg
     5641 cgacatcaaa tggaacttta ccaaattcct cgtcaaccgc gacggcgaag tggtcgagcg
     5701 ttttgcgcct agtgttacgc ccgaagaaat cgaagccgat attcgggcat tgctgtaatc
     5761 tgccgccgtc ttgggaaatg ccgtctgaag gcttcagacg gcatttgtcc gtttgcgggt
     5821 ttattccgaa atatcggcaa caccgctttc ggggaagatg ggcaaatccg gcggtgggag
     5881 cagggtatcc aaagtcaggg cggacgcatc gagtgtcggg tagccttcaa acgcgaccga
     5941 ataaccgctg tttccgttca tacggattcg ggcgtgcgcg cctagaggga aagtgatttt
     6001 gtcggcaatg tgtccgaacg ggaagcccgt cagcacgggg attttcgccg tgcgcgaaac
     6061 atggttggca acggcggaaa aatcataaga cggatcatag acatctcgaa ttttttccat
     6121 acggaaattg ccgaacacga tggcgcgctg tttcttcaaa atacccgaaa gatacagcgt
     6181 attgagcata cgttcgatgc ggtagggctg ttcgccgaca tcttcgagga acaaaatgcc
     6241 gccgtcgatg tcgggcatat aaggcgtacc ggcgagcgag gcgaggacgc ttaagttgcc
     6301 gccccacaac gtgccttcgg tttcgacatc ggcgcgttgg atataaggaa catcaacggt
     6361 caggcggttt tgggttgcac ccttgataaa ggcatccatc gtaaacgcac cgggggcggg
     6421 tttgccaaaa tcgctataag ccatcgggcc ggcaaaactc atcatattgc cttttgccaa
     6481 caatgccagc tggacggcgc atacgtcgct gaatccgaaa aacagcgtgc cgtgttcgcg
     6541 catccttgcg ccgagcgaag caaaatcgat atgcggcaga atgcgtgccg caccataacc
     6601 gccgcgcaag cccatcagca ctttaggtgt ggcaacgcga ccggaagcga catcttggaa
     6661 atcggcggca cgttgtgagt ccgtgccggc aaaccgttgg aaacggcggc tgcccgcctg
     6721 ttggttggtt acggtaaaac cgacattgta aaggcgggtt aaggctgtgt tgacgcggtt
     6781 ggtgtcttcg gcaaatcctg acgaagcgac aacgcgcaga aggttgccgt ccgaactttg
     6841 acggcgtggc gtttggagag gcacggttcg ggctttcaca acggaatggg aagagggaag
     6901 gggcggaacg gatgtggcgg atgtgccgca agcctgaagc agccccgcgc cggcagcggc
     6961 ggtgcaggtt ttcagaaaac ggcggcggga ggtcggttcg gtcataattt ccctttcata
     7021 cggtttcaga cggcatcggc gggcttaaag cagcttttta agctgttccg tccaatcttg
     7081 cggcagggtc ataccgtgtt tccggacggg gtttgcccaa atcggcgcgg ggaaggacgc
     7141 atcgttttca aagcgggcga taatatgcca gtgcaggtgc ggcacgacat tgcccaagct
     7201 ggcgaggtta atcttggcag gttgaaacac ttggcgcata gcggcttcga ctttgtacac
     7261 catttccatc aattcgccgc gttccgctgc cgaaaggtcg gtcatttcgg cgatgtgctt
     7321 acgccaaatg acgcggcaga atgcaggcga accgctgtcg ttatggatgg cgatgacgcg
     7381 gaggttgggg gtttgcagca aaatatcttc atcttgtgcc gtgcagatgg ggcagggttg
     7441 ggcggtcatt tttccttatt cctgaaagtg tttaaaaaac ggtaggataa tttct
//
LOCUS       DAWHXS010000093         7466 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWHXS010000093 DAWHXS010000000
VERSION     DAWHXS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.093"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..546
                     /locus_tag="WIE22_001633"
     CDS             <1..546
                     /locus_tag="WIE22_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEZ4127689.1"
                     /translation="LIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
     gene            complement(1104..1787)
                     /locus_tag="WIE22_001634"
     CDS             complement(1104..1787)
                     /locus_tag="WIE22_001634"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ4127690.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            2083..4386
                     /gene="parC"
                     /locus_tag="WIE22_001635"
     CDS             2083..4386
                     /gene="parC"
                     /locus_tag="WIE22_001635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ4127691.1"
                     /translation="MNMQPHAPHTDSNTLMLGQYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            4407..5924
                     /locus_tag="WIE22_001636"
     CDS             4407..5924
                     /locus_tag="WIE22_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ4127692.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQALRIPSFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYSSYAAILLIFNAIADGDIGKYPLISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDRAGKLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINTEGLAVLFNRKAKDLFPALEIGRRADLSDSAAELWDQASPHTFENVLGTLGLTA
                     RIRAVPVNKGPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTEREAIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHLTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            5976..6443
                     /locus_tag="WIE22_001637"
     CDS             5976..6443
                     /locus_tag="WIE22_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ4127693.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            6547..7299
                     /locus_tag="WIE22_001638"
     CDS             6547..7299
                     /locus_tag="WIE22_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ4127694.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1793 a         2326 c         1853 g         1494 t
ORIGIN      
        1 ctgattatcc cacgcgtctc atccgaaagc cgccgtttcg tccccatcgg ttatctatca
       61 tatgaaacag ttgtcagcga tttggtattt acccttccaa acgccagcct ctaccacttc
      121 ggcatcctca gctccaccat gcacaacgcc tttatgcgta ccgttgccgg ccgtctggaa
      181 agtcgttacc gctattccaa taccgttgtg tacaacaact tccccttccc cgaaagctgc
      241 cggatgccgt ctgaaaacga ccgccaagac ccgctccgcg ccgccgtcga aaccgccgcc
      301 caagccgtcc tcgacgcgcg cggacaatac cgccgagaag cgcgggaagc cggtttgccc
      361 gagccgaccc tcgccgaact ctatgcgccc gacgcaggct ataccgccct agacaaagcc
      421 cacgccgccc tcgacaaggc agtcgataaa gcctacggct acaaaacagg caaaaacacc
      481 gacgacgaag ccgaacgcac cgccttcctg ttcgagctgt accgcaaggc ggcggcaatt
      541 gcgtagtagt tgcgtagtag ttgcgtagta gttgcgtagt agttgcgtag gatagttgcg
      601 tagggtgggc ttcagcccac caactcccac caactcccac catcttcacc ctaaaacccc
      661 gtcatctccg caaaagcaat tgcgtagggt gggcttcagc ccaccgtttt tcttcatttt
      721 cggttgttga ttggcaggct gaagcccacc ctgcggttac aaaaatcccg tcattcccgc
      781 gcaggcggga atccagacct ttcggggcgg aaacttatcg gataaaacga tttcccctgc
      841 atcctggatt cccactttca tgggaatgac gggatatagg tttccctacg gacgtgttca
      901 gattcccgcc tgcgcgggaa tgacggcgga gcggtttctg cttttccgat aaatgaccgc
      961 aacctaaaac ccatccttcc cacgaaaaca gaaaaacaaa aacctaaaat cccgtcgttc
     1021 tcgcgaaagc agttgctacg tttaccgtca aaaatgccgt ctgaacgctc aagccttcag
     1081 acggcatttt ttatttcaca cccttacagg tagaattttt cgatgacttt caaattgtcg
     1141 tccaatttgt acaccaacgg ctgaccggtc gggatttcca agcccatgat gtcttcgtcg
     1201 gaaatgccct cgatgtgttt tgccagcgcg cgcagggagt tgccgtgcgc cgccaccaag
     1261 acgcgtttgc cgctcaaaat cgcgggggcg atttggtctt cccaaaacgg caatacgcgc
     1321 tccagcgtta cttgcaggtt ttcgccgtcg gggataacat cggcaggcag atgggcatag
     1381 cggcggtctt tgtgtgcgga aaactcatcg tctttgtcca aaagcggcgg cagggtgtcg
     1441 tagctgcgcc gccagatgcg gacttgctcg tcgccgtatt gttcggcggt ttgttttttg
     1501 tccaggcctt gcagtcggcc gtagtggcgt tcgttcagcc gccatgtttt gatttgcggt
     1561 acgaacagtt ggtcggattc ttccaaaacg atgttgcagg tcttaatcgc gcgggtcagg
     1621 acggatgtga aggcgatgtc gaactcatag ccgttttctt tcagtttttt accggcggca
     1681 gcagcctcgg caagcccctg ctcgctcagc ttcacgtcgc gccagcctgt aaacaggttt
     1741 ttcgcgttcc attcgctttg tccgtggcgg ataaatacca attccatatc gtctccaatg
     1801 tgtgaaagtg ggaaagcctt atttataaca tattttcaca ttcccgtatt tgattcagat
     1861 tcagacacgc gcccactatg gtttgccgtt ttgatttaca ataatgccct ttgctttaca
     1921 ttccgcatac acaatgtttc aacacacagg acgacacata aagcaccgcc ctatgtgttg
     1981 ccctgatttg gaaggggttg ccccctccca aataaagtct gattctaccg ccccgaagga
     2041 cagatgtcca agtggcgggg gttcaaccga aaaggaaata cgataaatat gcaaccgcac
     2101 gctccacata ccgattccaa tacgctgatg ctcggtcaat acgccgaacg cgcctatctc
     2161 gaatacgcca tgagcgtggt caaaggccgc gcgctgcctg aagtttcaga cggccagaag
     2221 cccgtgcagc ggcgcatttt gtttgccatg cgcgatatgg gtttgacggc gggggcgaag
     2281 ccggtgaaat cggcgcgcgt ggtcggcgag attttgggta aataccaccc gcacggcgac
     2341 agttccgcct atgaggcgat ggtgcggatg gcgcaggatt ttaccttgcg ctatccctta
     2401 atcgacggca tcggcaactt cggctcgcgc gacggcgacg gggcggcggc gatgcgttac
     2461 accgaagcgc ggctgacgcc gattgcggaa ttgctgttgt ccgaaatcaa tcaggggacg
     2521 gtggattttg tgccgaacta cgacggcgcg tttgacgaac cgctgcacct gcccgcccgc
     2581 ctgcctatgg tgttgctcaa cggcgcgtca ggcattgcgg tgggcatggc gaccgagatt
     2641 ccgtcgcaca atttgaacga agtgacgcag gcggcgattg cgttgttgaa aaagccgacg
     2701 ctggaaaccg ccgacctgat gcaatacatt cccgcacctg attttgccgg cggcggtcaa
     2761 atcatcacgc cggcggacga attgcgccgg atttatgaaa ccggcaaggg cagcgtgcgc
     2821 gtgcgtgcgc gttatgaaat cgaaaaattg gcgcgcggac aatggcgcgt catcgtaacc
     2881 gagctaccgc cgaacgccaa ttccgccaaa atccttgccg aaatcgaaga gcagaccaac
     2941 ccgaaaccga aagcgggcaa aaagcagctc aaccaagacc agctcaatac caaaaagctg
     3001 atgctggatt taatcgaccg cgtgcgcgac gagtccgacg gcgaacatcc cgtgcgactg
     3061 gtgttcgaac cgaaatccag ccgcatcgat accgatacct tcatcaacac gctgatggcg
     3121 caaacttcgc tggaaggcaa tgtgtccatg aacttggtga tgatggggct ggacaaccgc
     3181 cccgcgcaga aaaacctgaa aacgattttg caggaatggc tggatttccg caccgtaacc
     3241 gtaacacgcc gtctgaaatt ccgtttgaac caagtggaaa aacggctgca catcctcgaa
     3301 ggccgtctga aagtctttct gcacatcgac gaagtgatta aagtcatccg cgaatcagat
     3361 gacccgaaag ccgatttgat ggcggcgttc gggctgaccg aaatccaagc cgaagacatt
     3421 ttggaaatcc gcctgcgcca gttggcacgt ttggaaggtt tcaaactcga aaaagaattg
     3481 aacgaattgc gcgaagaaca aggccgtctg aacatccttt tgggcgatga aaacgaaaag
     3541 cgcaagctga ttatcaaaga gatgcaggcg gatatgaaac aatacggcga tgcacgccgc
     3601 acgctggtgg aagaggccgg acgcgccgtg ctgacacaaa ccaccgccga cgaacccatc
     3661 acactgattc tgtcggaaaa aggctggata cgcagccgtg ccggacacaa tctcgatttg
     3721 agccaaaccg cgttcaaaga aggcgaccgc ctcaaacaaa cccttgaagg cagaacggtt
     3781 ttacccgtcg tcatcctcga ttcattgggc agaacctata cactcgatgc cgccgaaatt
     3841 cccggcggac gcggcgacgg cgtgccggtt tcatccttga tcgagttgca aaacggcgcg
     3901 aaacccgttg cgatgttgac aggattgccg gaacaacatt atttattatc aagcagcggc
     3961 ggctacggct tcatcgccaa gttggctgat atggtcggac gcgtaaaagc gggcaaagtg
     4021 gtgatgacca cagacagtgg cgaaaccgtc ctgccgccga ttgccgtcta tgcctcctcg
     4081 ttcatcaacc ccgactgcaa aatcattgcc gccaccagtc aaaaccgcgc cctcgccttc
     4141 cccatcggcg aattgaaaat tatggcgaaa ggcaaagggc tgcaaatcat cggattaaac
     4201 gccggcgaat cgctgacgca taccgccgtt tcttccgagc cggaaatcct gattgaaagc
     4261 gaaggcaggc gcggcgcggc gcacaaagac cgcatccccg tctccctgct tgaggcaaaa
     4321 cgcggcaaaa aaggcaaact attacccata tcgggcagcc tgaaacagct ttcttccccc
     4381 aaataaaccc gattccacac ataattatgg tgatttccaa cccccgcgaa cttgaaaaac
     4441 tcaaagaccg gattcccaat ctgatcaaca tcatccgcgt cgccatcgtt tttccgctga
     4501 tgattatgca catcctcggg ctggaaaccg gcagccgtgc gaacctgcac gcttcgtgga
     4561 cggcgtgggc gttttatgtt tggctcgcca ttgcctgctg gctgattttc ttttccatca
     4621 tccatccgca ttggcaatgg caggctttga gaataccgag tttcagcgcg gtggcggaca
     4681 tcacgatgat cggcgtgctg acctacctgt tcggcggcat cgactccggc ttcggcatcc
     4741 tgattctgcc cttcgtcgtc tgctcctgcc tgctcagcta cgggcgctac cccctgctct
     4801 attccagcta cgccgccatc ctgctgatat tcaacgccat tgccgacggc gatatcggca
     4861 aatacccgct catatcggat gcccgaaccg cctcggcaac cttcatcctt gtcgccgcct
     4921 cctatctttc cgccatcttc acctcgctgt cggtcagata catcgaccgt gccggcaaac
     4981 ttgcccgcga caaccacctc gcctaccggc gcatcaaagg cttgagccaa accgtactcg
     5041 aacgcgttca agaagccgtc gtcgtcatca ataccgaagg gctggcggtg ctgttcaacc
     5101 ggaaggcgaa agaccttttc cccgcactcg aaatcggacg gcgcgccgac ctgtccgatt
     5161 ctgccgccga actgtgggac caagcctctc cgcacacttt cgaaaacgtc ctcggcacac
     5221 tcggcctgac cgcccgcatc cgcgccgttc cggtcaacaa agggccggac aagctgctca
     5281 tcctctacat ccgcccgcaa agcgaaattc aggcagaagc cctgtccgtc aaacttgccg
     5341 cgctcggaca actgaccgcc aaccttgccc acgaaatccg caacccgatg tccgccatcc
     5401 gccacgccaa cgacctgctg cgcgaaaata tggaagcggg ggcggcagat ccgttcaacg
     5461 ccaaattgtg caaaatcatc gacggcaacg tctgccgtat cgacaaaatg ctcgaagata
     5521 tttcctcgct caacaagcgc aacaaaaccg aacgcgaagc catcggcctg ataccgtttt
     5581 gggaagaatt caaacaagag ttcctgctcg gccatcccga tgccgccggc tgcatccgcc
     5641 tagatatgca gggcaatcac ctgaccgcct atttcgatcc cgcccacctg cggcaaatta
     5701 tgtggaacct cgccaacaac gcgtggcggc acagccgcaa acagcccggc tcgatttccg
     5761 tcaccatccg ccccgcgcaa aaaaacaccg tctgtatcct ctttgccgac cgcccgaagt
     5821 gcaggaacac ctgttcgaac ccttttacac cacggcggaa aacggcaccg gcctcgggct
     5881 gtatgtcgcc cgcgaactgg cgcacgccaa tttcggcgat ttgacctacc taccggaagc
     5941 caaatgtttc gaactcacat taccggaaaa aaccaatgac tgaactgcaa caccccgtcc
     6001 tcgtcgtcga tgacgaaacc gacattctcg acctgatgga aatgaccctg atgaaaatgg
     6061 gcttgcgcgt ccatactgcg tcaggcgttg ccgaagccaa aaacaagctc gacagccaac
     6121 gctattcgct cgtcctgacc gatatgcgta tgccggacgg ctcggggctg gaagtcgtcc
     6181 aacacatcaa cagccgcctg ctcgatacgc cggttgccgt catcaccgcc ttcggcaacg
     6241 ccgatcaggc acaggaagcg ttgcgttgcg gcgcgttcga ccccgatacc atgcagatac
     6301 aggactatct cgaccaaatc gaacgcgaca ttatcgaaca aaccctcaaa caaaccgaag
     6361 gcaaccgcac gcaggccgcc aaacgcttgg gcatcagctt ccgttccatg cgctaccgta
     6421 tggaacgcct caacatcggc tgacgacaaa acggcatccg caccatctcc gcccacccga
     6481 aaaaatgccg tctgaaacgg cacgggaaag cgggttcgcc ccacgcccga acggacacaa
     6541 aacaccatga ccgacatcct tattgacaac accgccaccg aaaccgtccg caccctgata
     6601 cgggcattcc cccttgtgcc cgtttcccaa ccgcccgaac aaggcagtta cctccttgcc
     6661 gaacacgata ccgtcagcct caggcttgtc ggggaaaaaa gcagcgtcat cgtcgatttt
     6721 gcctccggcg cggcacaata ccggcgcaca aaaggcgggg gcgaactcat cgccaaagcc
     6781 gtcaaccaca ccgcgcaccc caccgtttgg gacgcaaccg caggattggg gcgcgacagc
     6841 ttcgtcctcg cctcgctcgg gctggccgtt accgccttcg agcaacatcc cgccgtcgcc
     6901 tgcctgcttt cagacggcat ccgccgcgcc ctcctcaatc ccgaaacgca agacaccgcc
     6961 gcgcgcatca acctccattt cggcaacgcc gcagtacaaa tgcccgccct tgttcaaaca
     7021 caaggcaaac ccgacatcgt ctatctcgac cccatgtatc ccgaacgccg caaaagtgcc
     7081 gccgttaaaa aagaaatgac ctacttccac cggctcgtcg gcgaagcgca agatgaagcg
     7141 gcactcctgc ataccgcacg ccaaacagca aaaaaacgcg tcgtcgtcaa acgcccccgc
     7201 ctcggcgaac accttgccgg acaagcccct gcctaccaat acaccggcaa aagcacccgc
     7261 ttcgacgttt acctgcccta cgggacggac aagggataac gcccataaaa caagacaccg
     7321 aaaaatttgc cgttcttatg caaacgagaa accggttttt gcgtttcgac tgttttggat
     7381 aaatcatcac accttaaagt ttgtcattcc cacggaagtg ggaatccaga aatgaaaagc
     7441 aacaggaatt tatcgtaaat gaccga
//
LOCUS       DAWHXS010000094         7443 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWHXS010000094 DAWHXS010000000
VERSION     DAWHXS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.094"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            144..1154
                     /gene="dusB"
                     /locus_tag="WIE22_001639"
     CDS             144..1154
                     /gene="dusB"
                     /locus_tag="WIE22_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ4127695.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFTDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
     gene            1184..1423
                     /locus_tag="WIE22_001640"
     CDS             1184..1423
                     /locus_tag="WIE22_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ4127696.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYNMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            1426..1962
                     /gene="ruvC"
                     /locus_tag="WIE22_001641"
     CDS             1426..1962
                     /gene="ruvC"
                     /locus_tag="WIE22_001641"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ4127697.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            2013..2882
                     /locus_tag="WIE22_001642"
     CDS             2013..2882
                     /locus_tag="WIE22_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ4127698.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAKRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKGSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREEDNTVTLHFYPAWESFPSEDAQADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            3629..4423
                     /locus_tag="WIE22_001643"
     CDS             3629..4423
                     /locus_tag="WIE22_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ4127699.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFAQAWK"
     gene            4635..7238
                     /gene="pepN"
                     /locus_tag="WIE22_001644"
     CDS             4635..7238
                     /gene="pepN"
                     /locus_tag="WIE22_001644"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ4127700.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIAGVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GGFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVAAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRATMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTVKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPPEAELWDGT
                     ENIDPLCYHQAHEALLDTIAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTSLQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
BASE COUNT         1849 a         1962 c         1974 g         1658 t
ORIGIN      
        1 ccgcctgtga gtggcggggc agggaatctg tgtgcggatt atgccataaa acggtgtccg
       61 ccccaatcgc gggcgcgtcc ggagattgga aatcctgctt aaaaaatgta caatggcgta
      121 cttttttgaa acgcggatcc attatgcaca tcggcggcta ttttatcgac aaccccatcg
      181 cacttgcacc gatggcaggc attaccgaca agccgttccg tcgcctttgc cgggcgtttg
      241 gcgcaggttg ggcggtgtgc gaaatgctga ccagcgaccc gacgcttaga aataccagaa
      301 aaaccctgca ccgcagcgat tttaccgatg aaggcggcat cgttgccgtg cagattgccg
      361 gcagcgatcc gcagcagatg gcggatgcgg cgcgttacaa cgtcagcctt ggggcgcagc
      421 ttatcgacat caatatgggc tgtcccgcca agaaagtctg caatgtccaa gccggtagcg
      481 cgctgatgca gaacgagccg ctggttgccg ccattttgga agcggtggtc aaggcggcgg
      541 gcgttcccgt taccctcaaa acccgtttgg gttggcacga cgaccatcaa aacctgcccg
      601 tcatcgccaa aatcgccgaa gattgcggca tcgccgccct tgccgtccac ggacgcacgc
      661 gcacgcaaat gtacaaaggc gaagcgcgtt acgaactcat cgccgaaacc aaatgccgtc
      721 tgaacatccc ggtctgggtc aacggcgaca ttacttcgcc gcaaaaagcc caagccgtcc
      781 tcaaacaaac cgccgccgac ggcattatga tagggcgcgg cgcgcaaggc aggccgtggt
      841 tcttccgcga tttgaaacat tatgccgaac acggtgtttt gccgcctgcc ttgagtttgg
      901 cagaatgcgc cgccgctatt ttgaaccaca tccgcgccat acacgcgttt tacggcgaca
      961 ccgccggtgt gcgcatcgca cgcaaacaca taggctggta catcgacgaa atgcccgacg
     1021 gcgaacagac acgtcgtgaa atcaaccgct tggacagtgc ggcggcgcaa tacgacatgc
     1081 ttgcaggtta tcttgaaaga cttgccgaaa aaaccgacag ttgggcgtgc gcctaccgcc
     1141 caaatgcctt ctgaacactt gattatcctt tgaaagtgca atcatgcccc atacccttcc
     1201 cgatatttcc caatgcatca gacaaaattt agaacaatat ttcaaagacc tgaacggtac
     1261 cgaaccttgc ggcgtgtaca atatggtatt gcatcaggtg gaaaaaccgc tgctggtgtg
     1321 cgtgatggag caatgcggcg gcaaccagtc caaagcctcc gtcatgttgg gactgaaccg
     1381 caataccttg cgtaaaaaac tgattcaaca cggtttgctg tgaatatgtc ggcaaccgtc
     1441 cgtatcttgg gcattgaccc gggcagccgc gtaacgggtt tcggcatcat cgatgtcagg
     1501 gggcgcgatc atttttacgt tgcctcaggc tgcatcaaaa cgcctgccga tgcgcctctg
     1561 gcagacagga ttgccgtcat cgtccggcac atcggcgaag tcgttaccgt ttacaagcct
     1621 caacaggcgg cagtggaaca ggtgttcgtc aacgtcaatc cggcatcgac gctgatgctc
     1681 ggtcaggcta ggggcgcggc attggcggca ttggtcagcc ataagctgcc cgtttcggaa
     1741 tacacggcct tgcaggtcaa acaggcggta gtcggcaagg gcaaggcggc aaaagaacag
     1801 gtgcagcata tggtggtgca gatgctcggc ttatcgggaa cgccgcaggc ggatgcggcg
     1861 gacggtcttg ccgtcgcgct gacccacgcc ttacgcaacc acgggcttgc cgccaaactc
     1921 aatccttcgg ggatgcaggt caagcgcggc aggtttcaat agtttcagac ggcatttgta
     1981 ttttgccgtc tgaaaagaaa atgtgtatcg agatgaaatt tatatttttt gtactgtatg
     2041 ttttgcagtt tctgccgttt gcgctgctgc acaaacttgc cgacctgacg ggtttgctcg
     2101 cctacctttt ggtcaaaccc cgccgccgta tcggcgaaat caatttggca aaatgctttc
     2161 ccgagtggga cggaaaaaag cgcgaaaccg tattgaagca gcatttcaaa catatggcga
     2221 aactgatgct tgaatacggc ttatattggt atgcgcctgc caaacgcctg aaatcgctgg
     2281 tgcgttaccg caataagcat tatttggacg acgcgctggc ggcgggggaa aaagtcatca
     2341 tcctgtatcc gcactttacc gcgttcgaga tggcggtgta cgcgcttaat caggatgttc
     2401 cgctaattag tatgtattcc caccaaaaaa acaagatatt ggacgaacag attttgaaag
     2461 gccgcaaccg ttatcacaac gttttcctta tcgggcgcac cgaagggctg cgcgccctcg
     2521 tcaaacagtt ccgcaaaggc agcgcgccgt ttctgtatct gcccgatcag gatttcggac
     2581 gcaacgattc ggtttttgtg gattttttcg gtattcagac ggcaacgatt accggattga
     2641 gccgcattgc cgcgcttgca aatgcaaaag tgatacccgc cattcccgtc agggaggagg
     2701 acaatacggt tacattgcat ttctaccctg cttgggaatc ctttccgagt gaagatgcgc
     2761 aagccgacgc gcagcgcatg aaccgtttta tcgaagacag ggtgcgcgaa catccggaac
     2821 aatatttttg gctgcacaag cgttttaaaa cccgtccgga aggcagcccc gatttttact
     2881 gactacgtaa aattacaaaa catatcaggc gtttcaagtc gaaattcctg attgtttttg
     2941 gaaatttgaa atccgggtcg tgcaaacagg atttgccgga cggttttaac ggttcagttg
     3001 tttgtaaaaa caatgctttt taagattgac aaaaaaccag aatcaggttt tagggcttat
     3061 tccgcgaaca aagggggcgg atgccgaaaa tccgattaat atattaaaat taaatttgtt
     3121 taaaaacata aagttgtaaa caagtatctc atataagcct ttttcattaa acagatagtc
     3181 agatattttg tgctaaaaat ttatataata tttaaattaa tatcaagtta taaaaatata
     3241 tagaatttta ttttgtttat ttataatttt aagcaataat ctaaatcagg cattttgatt
     3301 cccctgtttt aaaaaatact tggcaaggtg tgaaaaaagg cgtacattcc gctacacaga
     3361 attacagacg cagcggagca atgccgtctg aaaggatttt ccggtcagtc ttgcgattgg
     3421 tcggggtttc atcggaaacg gtgaaacgaa agtttgccgg cgtagagttg agctacgcgg
     3481 gtaaagccgc aggcgaaagc ctgtattgtt tgtgaagcgt aaatctctga tttgaggtat
     3541 tggggcaatc ctgtgggggg ttgcctcttt ttttatccgc cttttaatga cacaataggt
     3601 gcatccgttt taaatacaag gtgctgtcat gaccacgatt ttggctttcg atattgaaac
     3661 cgtacccgat gtgcagggta tccgaacatt gtacgagctg ccgtcctcgc tgcccgacga
     3721 tgaagtggtg ctgtttgccc agcaaaaacg ccgtgctcag acgggtgggg attttatgca
     3781 gcatcatctt catcagattg tggcgatttc gtgctgcatg cgttgggggc aggataaagt
     3841 ccatgtcggc accatcggcg agatggacga tggcgaagaa gtcgtgattg ccaaattttt
     3901 cgagttgatt gaaaaacata cgccgcaact ggtcagttgg aacggcggcg gtttcgacct
     3961 gccggttctg cattaccgct ccctgatata cggcattaac gccgcgcgct attgggatat
     4021 gggcgacggc gatttcggcg acagccgcga tttcaagtgg aacaactaca tcagccgtta
     4081 tcaccaacgc cactgcgatt tgatggattt gctcgcgctt taccagccga gggcaaacgt
     4141 gccgctggac gatatggcga aactgtgcgg ttttccgggc aagctgggta tggacggcag
     4201 caaggtgtgg gatgcgttcc acgcgggcag gctgaaagaa atccgcaatt attgcgaaac
     4261 cgatgccgtg aatacgtatt tgatgtatct gcgtttttgt ctggtcagcg gcagattcga
     4321 cgcggacgaa tacgaaatgg aaatcaagcg gatcagaaac tatctttccg cccaaacaga
     4381 agataaaccg cattggtcag aatttgccca agcgtggaaa tagaaaacct gctgtccgaa
     4441 ggcggtacgc ttgtgccgcc gtttcggttt cagacggcat atggtggatt aaattcaaac
     4501 cggtacagcc cgtactatct gtactgtctg tggcttcgtc gccttgtcct gatttttgtt
     4561 aatccgcttt aaaagctgta tccgtgctgt ctgaagcctt ttccagtcaa accaaacaat
     4621 attgaaagaa aaaaatgagc aaaaccgtcc gttatctgaa agattaccaa acgcctgcct
     4681 accgcatcct tgaaaccgac ctgcattttg atattgccga accgcaaact gtcgtgaaat
     4741 cccgtttgac ggtcgagccg cagagggtgg gggagccgct ggtgttggac ggttcggcga
     4801 aactcttgtc cgtcaaaatc aacggggcgg cggcggatta tgtgttggaa ggtgagacgc
     4861 tgacgattgc aggcgtgcca tccgaacgct tcaccgtcga agtggaaacc gaaatcctgc
     4921 cggcggaaaa caaatcgctg atggggttgt atgcttccgg cggcaatctg tttacccagt
     4981 gcgagccgga gggcttccgc aaaatcacgt tctacatcga ccgtccggat gtgatgtcca
     5041 aattcacgac caccatcgtt gcggacaaaa aacgctatcc cgttttgctc tccaacggca
     5101 acaaaatcga cggcggcggg ttttcagacg gccgccattg ggtgaaatgg gaagacccgt
     5161 ttgccaaacc gagttatctg tttgctttgg tcgcgggcga tttggcggtc acggaagact
     5221 atttcaccac catgagcggc agaaacgtca aaatcgagtt ttacaccacc gaagcggaca
     5281 agcctaaggt cggctttgcc gtggaatcct tgaaaaacgc gatgaagtgg gacgaaacgc
     5341 gcttcggttt ggaatacgac ttggatattt tcatggtcgc cgccgtgggc gatttcaata
     5401 tgggtgcgat ggaaaacaag gggctgaaca tttttaatac caagttcgtc cttgccgaca
     5461 gccgtaccgc caccgatacc gattttgaag gcatcgaatc cgtggtcgga cacgagtatt
     5521 tccacaactg gacaggcaat cgcgttacct gccgcgactg gttccagctt tcactgaagg
     5581 aagggctgac cgtgttccgc gaccaagaat tttccggcga ccgcgccagc cgcgccgtgc
     5641 gccgcatcga gaacatccgc ctgctgcgcc agcaccagtt ccccgaagac gcaggcccga
     5701 ccgcccatcc ggtgcgcccc gccagctatg aggagatgaa caatttctac accatgaccg
     5761 tttatgaaaa aggcgcggaa gtggtgcgga tgtatcacac cctgctcggc gaagagggct
     5821 tccaaaaagg catgaagctc tatttccaac gccacgacgg gcaagccgtg acctgcgacg
     5881 atttccgcgc gacaatggcg gacgcgaacg gcattaatct cgaccagttc gccttgtggt
     5941 acagccaggc gggcacgccc gttttggaag cggaaggtcg tctgaaaaac aatgttttcg
     6001 agttgaccgt caaacaaacc gtgccgccca cgcccgatat ggcggataaa cagccgatga
     6061 tgattcccgt caaagtcgga ctgttgaacc gcaacggcga agcggtagca ttcgattatc
     6121 agggcaaacg cgcaaccgaa gccgtattgc tgctgaccga agccgaacag atcttcctgc
     6181 tcgaaggcgt aaccgaagcc gtcgttccct cgctgctgcg cgggttcagc gcgccggtgc
     6241 atctgaacta tccgtacagc gacgacgacc tgctgcttct gctcgcccat gacagcgacg
     6301 ccttcacgcg ctgggaagcc gcccaaacgc tctaccgccg cgccgttgcc gccaaccttg
     6361 ccgcgctttc agacggcatc gggttgccga aacacgaaaa actgcttgcc gccgtcgaaa
     6421 aagtcatttc agacgacctc ttagacaacg ccttcaaagc cctgcttttg ggcgtgccgc
     6481 ctgaagccga gctgtgggac ggcacggaaa acatcgaccc gttgtgctac catcaggcgc
     6541 acgaagcctt gttggataca atcgccgtcc gcttcctgcc gaaatggcac gaattgaacc
     6601 gtcaggcggc gaagcaggaa aaccaaagct acgagtacag ccccgaagct gccggctggc
     6661 gcacgctgcg caacgtctgc cgcgccttcg tcctgcgcgc cgaccccgcg cacatcgaaa
     6721 ccgttgccga aaaatacggc gaaatggcgc aaaacatgac ccacgaatgg ggcatcctgt
     6781 ccgccgtcaa cggcaacgaa agcgatacgc gcaaccgcct gctggcgcag tttgccgaca
     6841 agttttcaga cgacgcgctg gtgatggaca aatatttcgc cctcgtcggc tcaagccgtc
     6901 gcagcgacac cctgcaacag gttcaaacct ccttgcagca tccgaaattc agcctcgaaa
     6961 accccaacaa agcccgttcg ctcatcggca gcttcagccg caacgtcccg catttccacg
     7021 cagaagacgg cagcggctac cgcttcatcg ccgacaaagt catcgaaatc gaccgcttca
     7081 acccgcaggt cgccgcccgc ctggtgcagg cgttcaacct ctgcaacaag ctcgagccgc
     7141 accgcaaaaa cttggtgaaa caagcattgc agcgcattcg ggcgcaggaa ggattgtcga
     7201 aagacgtggg cgaaatcgtc ggcaaaattt tggattgagg ccgtcaaaca gaaaaacaat
     7261 gccgtctgat atagttacac acacgtttta tcatcacttc cccatcgttt tattacgcaa
     7321 tggcaaaacg gccgcaaagt catattaatt atagtggatt aacaaaaatc aggacaaggc
     7381 gacgaagccg cagacagtac agatagtacg gtaaggcgag gcaacgctgt actggttttt
     7441 gtg
//
LOCUS       DAWHXS010000095         7345 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWHXS010000095 DAWHXS010000000
VERSION     DAWHXS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.095"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(179..391)
                     /locus_tag="WIE22_001645"
     CDS             complement(179..391)
                     /locus_tag="WIE22_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4127701.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(403..684)
                     /locus_tag="WIE22_001646"
     CDS             complement(403..684)
                     /locus_tag="WIE22_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127702.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(731..994)
                     /locus_tag="WIE22_001647"
     CDS             complement(731..994)
                     /locus_tag="WIE22_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127703.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1014..1586)
                     /locus_tag="WIE22_001648"
     CDS             complement(1014..1586)
                     /locus_tag="WIE22_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ4127704.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1565..1876
                     /locus_tag="WIE22_001649"
     CDS             1565..1876
                     /locus_tag="WIE22_001649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127705.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1898..2773)
                     /locus_tag="WIE22_001650"
     CDS             complement(1898..2773)
                     /locus_tag="WIE22_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ4127706.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2818..3108)
                     /locus_tag="WIE22_001651"
     CDS             complement(2818..3108)
                     /locus_tag="WIE22_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ4127707.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3120..3299)
                     /locus_tag="WIE22_001652"
     CDS             complement(3120..3299)
                     /locus_tag="WIE22_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ4127708.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3413..3790
                     /locus_tag="WIE22_001653"
     CDS             3413..3790
                     /locus_tag="WIE22_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ4127709.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3819..4064
                     /locus_tag="WIE22_001654"
     CDS             3819..4064
                     /locus_tag="WIE22_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127710.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4298..5266
                     /locus_tag="WIE22_001655"
     CDS             4298..5266
                     /locus_tag="WIE22_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127711.1"
                     /translation="MAKKTTKTIHYLRAQQNHSLFDLEVALRRVLSAAPTVQDTQIEQ
                     YNQLTQIMHRTLDPKDVKGNSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPP
                     SGYSFLRKEAFLYIVGHHVIFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFK
                     AVGNCDKLSLIQQHGVKSILLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSD
                     EELEAQSEIHINLEVLLNGNSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVI
                     QPSDVKLSKSVRIGRYDEANSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5263..5745
                     /locus_tag="WIE22_001656"
     CDS             5263..5745
                     /locus_tag="WIE22_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127712.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            5835..6338
                     /locus_tag="WIE22_001657"
     CDS             5835..6338
                     /locus_tag="WIE22_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ4127713.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            6447..6779
                     /locus_tag="WIE22_001658"
     CDS             6447..6779
                     /locus_tag="WIE22_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127714.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAAVPQLAEVKKS
                     ANQEGGRQ"
     gene            6776..7186
                     /locus_tag="WIE22_001659"
     CDS             6776..7186
                     /locus_tag="WIE22_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ4127715.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            7191..7343
                     /locus_tag="WIE22_001660"
     CDS             7191..7343
                     /locus_tag="WIE22_001660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127716.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDETFA
                     KFPKIP"
BASE COUNT         1794 a         1735 c         1842 g         1974 t
ORIGIN      
        1 atttggggcg aaaacagctg aaaacctgtg tttgggtttc ggctgtcggg agggaaagga
       61 attttgcaaa ggtctcagag tgagtttatt ttggggcggc ggcaggtcgg gggcaagcgg
      121 tgtggggctt ggttgtggtt tttaggtttt tgggggtaaa aaatgccgtc tgaacttttc
      181 agacggcgtt tgttttttct gtccaatcga ggaactgccg ccatttttcc agcggcatat
      241 cggcccgccg cgtttcggta tcgggttcgg cttcccagcg gcctacctgt atgtagtgct
      301 tcaccccgac gatttgcgcc aactcggcct gtgtcagttt gcagcggttg cgcagggtgc
      361 gaaggttgta aggcgtgtag ccgagttcca tgtcgttgcg gtttattttg tttcgcatat
      421 ttttttgact gcccggcggc aggtttcggt aaggacggcg gcaaatcggg cttcgtctgc
      481 cggttttccg tcgaaaaaaa taaaatcgta aagggtgatg ccgttttcgg aatggcggta
      541 atgcccgccc gggcattcgc tgtctgtctg cctgtggatt ttggcaatca ggcggggata
      601 gcggcaatgg gtaatgaaac ggcttgcgcc gtcttgcccg ataatccatt cggggtagcg
      661 gttgaatagt gcggctctgt tcattttgtt cgggttgttc gggggataaa gcccctcgcg
      721 gggcttgtgg tcaggcaaat ttgaatatca gtgccaacac ggcggcgatg gcggtaacca
      781 gcccggtggc cgccatcatg ggataccatc gggattcttg ggctattttt acggattcgg
      841 cgttgatttt gtgcgcgtct gcaatgattt tggcgatttc ggcatctact tttctcagac
      901 tggagtgttt catttcttgt tcgattatgt tcatcgtact tcctttcgtt tttggcggtt
      961 gccgccgctt gtcggatggt aggatgtctg ccatgtgtat atattgatac cttttaggtt
     1021 ttattgcaag tgttttgggc ggcggcttcg tatgcttggc ggtggcggcg gctgtaccat
     1081 tcggcgagtt cgcgccgctc ttggaggcgg tagctgtcgg cggtcaggta gtggtgcagg
     1141 cttgagaagc cggcgcgttg gagggcggcg cggctgatgt ggccgagggc gaggtctgtt
     1201 gtgagggtgt cttttccggc ggtcaggctg atgttggtga agaggtggcg gaatccgtgc
     1261 attgtgtgtt tggatttgcc gggggtgctg ccgtcatagc ccagtcgtcg gatggcgttg
     1321 tgggcgaatt tgatgctgat gtggtcggga tggggggcgg gtttgcgccg tgggcggatg
     1381 ccggggaaaa ggtggatgtt gtcgccggtc tgtgtgtgca gctctcggag tatttctacc
     1441 gcccagtccg acagtgggac ggtaaagggg tgtttggtct tcatgtcggc ggcggggatg
     1501 tgccataacc gggcggtgag gtcgatgtct tgccagcggg cggaaagcag tgcggaggga
     1561 cgggatgact tccccggttt ccccatcaat ccgcgccgcc tcgatttttc cgttggcgcg
     1621 cagcatttcc accgcctgcc gcaccgacat caaccgcttg ccgttgcgcc tcatcgcctc
     1681 caccaaaacc gccgaaatca atttggcttc ttccggttta agctccgtct tgcccgcatc
     1741 gctgcgccgt ttgcgcgtcg gcttgacgct gaccgcctcc agcttgcggt atagcgtggc
     1801 aaggctgatg cccaattcct gcgcctgctg cttaagatat gcagagcgtg cgccgcgttc
     1861 cattgcttcc gcctgattct cgactgcctt aagacgctca atcattgccg gattcatcgc
     1921 cttctcccgt ttcaccgccc aaccattccg gcacattgtc tgtcggtgct tcagtcggca
     1981 gggcatagct ttcgcgcagt tgctcgcagt ccaaaataat ttgattgagc gtgccgacca
     2041 tctttgcctg atggctgatc ccgtgtgcct cactgtgcgc attaagttgg tcgaacaaat
     2101 ctttcagacg gctcacttga ctgcggatac cgacctcaag gcttgttaac tgcatcgcca
     2161 actcgctgcc tacgtcttcc gccttcggct ctctgacaac ggtttgcttc ttagccagct
     2221 tctcggccag ctcatcaatt ttggctgttt tggttttcat cacttcgtct ttggcggcga
     2281 ggttttcgcg gctttcgcgc agggcgacgc gcagctcgcg caccgtcatt cggtccacat
     2341 cgtcaaaggt catgccgttg acttcttccc cttcggccaa acccaccagc gtaacgtctt
     2401 cttcgaccaa gagttccagc agcttcgact tgcccaaatc catcagcttc ggcgcggctt
     2461 tctgcatttg cggggtcgca aagcggcgag tggctgacat cagacgtgat gtttctgcga
     2521 tacccagccc aaattggctt ttcacaattt ccataaaccg tccgtgttcc gtatgctctt
     2581 ttaaaataat cagcgcacgt cccagttcga acatgccttc catcgtctgc cgtactgctt
     2641 gacgaccgcg ttcaacccaa cgctcttcgc tataagtctc gccgttaccc cactgctcca
     2701 tcaccagcac actgcgcatc gctgcctgat tgcttacttt gtcggttgcg ataatttcaa
     2761 tttctgtatt cattttttat ctccaaagtt tccgacgtcg gaaactttca aaatccgtta
     2821 atccacatcg acccgcttgc cgatttcggc aatcttgctt tgcagccgtt catgctgctg
     2881 cctgaaccgc tctgcgattt gcagggtttt gatgccgtag gcgtagttgc cgttttcaag
     2941 tttgatgacc aatcccgagg caaccaaatc atcaatatcc ctgctgactt gcgatggcgt
     3001 cagccccagt ccgaccgata aatccttatt gctcagaccg ataatcggat gctcgtcaag
     3061 cgcgataaag accctcaata gccgttgtac ccttttactt tctgccatcc gcatcctcct
     3121 tatttcagtc ccagcttctt ggcaatttca tgccccttgc cgtaattgcc tttgcgctgt
     3181 ccgccgacca ccagatacac atcgcgcggc ttaaagccgt tctctcttgc ccactgcgcc
     3241 agcgtttggc cgttttgggc aaaattttct tttagttttt caacagtata gccatagata
     3301 gccctttccc attgtgataa aatagtgaac tatttatgca ctagaaatta attagtgcat
     3361 taccaatgcg tgaattatga gattttaaaa tctcttttac aaggattatt ttgtgatttc
     3421 taaatctctt tttggtaatc gtttgaaaga aaaaagaaag tttttagggc ttacccaagc
     3481 acaagctgct gaaaaagcag gtattgagcg cgaaacatgg ggcaaatacg aacgtggcgt
     3541 atttatgcct agtggagatg tattgctctc ttttttgaat atgggaatag atgtgagcag
     3601 cttatttgct gctgaacagg gaattaggcc gtctgaaaat tctgaaatta gtaaggaaga
     3661 gtcagagtta ttagggtatt accgccaagc cagcgataac ggcaaatttg tcattctcag
     3721 tgtggcaaaa ggcgcagaga aaaaagcagc cgaaaatact caagtttcag ccacgcataa
     3781 aacagcgtga tttttaaatc caaaatggag acagtcttat gtttgacttt gaacaacaaa
     3841 tcaaatgggg tgagcgagcg gaagaaatcg ttaaagaggc agccacccag aacaatatcg
     3901 aaattcccga acctttggct tcggcattag ctaaggcggt aaaagtgcat tatctaagcc
     3961 aagctggtgt gttttctttg gtagaagcct atgcagacac ggtaaatcca actgaaaaag
     4021 aagtagatta ccaagcaatc ggcaaagagc tatttgaaaa ataaaaaatg ccgtctgaaa
     4081 ttttcagacg gcatttagga cactccgaca gtcgggctgc tccggtgttc tgaaacggat
     4141 attaagcaat tgaagcggcc gtgcatttta aaacgcatta aaaactaccc cgacatacag
     4201 gtcatcgggc aggtggttca ggtgttgaaa gatttgaatt gatgccgtct gaatttattt
     4261 tttcgacaca gatattaaaa gagaaaggac tgtacaaatg gcaaaaaaaa ctactaaaac
     4321 tattcattat ttacgtgcgc agcaaaacca tagcttgttc gatttagagg tagcattaag
     4381 aagggttttg tcagctgctc ctactgttca agatacacaa attgaacaat acaatcagct
     4441 tacccaaatc atgcatagaa ctttggatcc taaagatgta aaaggcaatt caattggtgg
     4501 attactgata cacattggca gcggtacaaa agatgaacat atccgcacta tgagcaatag
     4561 acctgtacag caagatgatc atggtggaac acaagctcct ccaagtggat attccttttt
     4621 gcgtaaagag gcttttttgt atattgttgg acaccatgta attttttgcg gacatggttt
     4681 tttgtcagca tcaacggttg cttcttattt aagtttactt agcaataaat tgagagagaa
     4741 caatccggat attgttcaat ttaatattga attcaaagca gtaggcaatt gcgacaagtt
     4801 gtcattgata caacaacatg gggtaaaaag tattctttta gatgcttcgg catatcaatt
     4861 atccagaaac agactgtatc aagatagccg ttcaaccatt gctaaagcat tagggaaagt
     4921 tggtagtgta tttacaagcg aattgagtga tgaggaattg gaagcacaat ccgaaattca
     4981 tatcaattta gaagtcttgc taaacggtaa ttcgagagcc agtattgaag cgcaatcgct
     5041 gatgcaggag caggcagagg aaattattga tgatgaaacg gttaatcaag gattttccat
     5101 taccacgcaa caaggtgaag tgattcagcc ctctgatgta aaactgtcta aatctgttag
     5161 aataggacga tacgatgaag ccaactcgtt actccctgac tcggcattta cagcaatcag
     5221 tgaatatttc ctagaactcc aaagcagaaa cttaactgaa caatgaaaaa atatctgccc
     5281 ttactacgtt ttttgttgat tgcttgtata tcagcatttt tagcatggaa agggcaacct
     5341 tttgttcatg ggaacgagaa ggccgttgat ttaattatta acgtatttgc aatcttagcc
     5401 ggttttttaa ttgccattat gacattgttt agtgatatga ggtttgatga agatgcaaat
     5461 tggcgacaaa ttcaaattcg tgaaggcgta caagaacaac ggtatataaa acattcctta
     5521 cttttttaca catatcttgc cgtattagtt tgtgttttta ttgtcatctt gttggctcat
     5581 aaagaagaat acaaaaatgg accagccatt ttttggcttg aacggagcta tttattttta
     5641 gcctgtattt ccatttttta ttctgtattc ttacctggga atttaattaa aagccgaaaa
     5701 gaagagttta aaaaattaat ggaaaagaag aaacccaaaa tctagaacga gttttaaccc
     5761 cctttaaaat cccattcaga cgaccttttc taaaatccct gtattgattt ttaattcaat
     5821 acagggattt ttccatgtca gacaagttca accaattcat caaccgcgtc ctctctcacg
     5881 agggtggtta cgccaaccat cccaaagacc ccggcggcga aaccaattgg ggcatcacta
     5941 agcgcaccgc acaggcaaac ggctacaacg gctccatgcg tgccatgacg cgtgaacagg
     6001 caatcagcat ttaccgtaaa gcgttttggg agcgttaccg cgccgaccaa atgcccgaag
     6061 cggtcgcttt tcaatttttt gatgcctgcg tcaaccacgg ttacggcaat gccgcccgta
     6121 tgctccaacg cgccgcaggc gtgccggacg atggaatcat cggcgaaatc agcctcaaag
     6181 ccatcaattc ccttcccgaa aacgaccttt tattgcggtt caacgccgag cgtctggtct
     6241 tttataccaa gctgggtacg ttcacctctt tcggcaaggg ctgggtacgc cgtgtggcgc
     6301 aaaacctgat tcacgccgcc gcagacaatg ccgattgagg agcaaaccat gtcaaaaaag
     6361 tccattctcg ccctgatggc cgcagccatg cagcccgatt tcagccacag cgacctaggc
     6421 attcgctacg ccatgccgac tcagggatgt tggacgcaag cccaccgcaa gagcggggta
     6481 gccgccgcga aacgcgcagc caaaaaaaag cgtcacaaat aaccgccttt ttccgatggc
     6541 tgggcggctt ggtctctaat ccggccacag ggaaaatcag ccataccaaa ctttgggcaa
     6601 acgtcgccgc agccgccatg acttggaagt tcgtgcaggc ggcggacgcg cccgaatggc
     6661 tttggtgggc atacggcgcg atggtcggcg ggtatgcatt aatcaaacgc ggcatcgccg
     6721 ccgtaccgca gttggcggaa gtcaaaaaat ccgcaaatca ggaagggggg cggcaatgat
     6781 tgaatttgtc cgagccaaaa aacggctgct ttgggcattt gtgcttttgc ttgtgtggac
     6841 gtgcggttac cgatacgccg ccgacaaggc cgaagcggaa caaaccgccc tgattgccac
     6901 ctatcggcat tcttctatgg ttgcggcgga acaatatgcc ttgcagctta aaaaagcgca
     6961 ggacgaaagg cagcggtggt acgacttttc ccaaaaacaa ggaagaaagc ccgtgaaaaa
     7021 acagtatccg ccgcaaacga aaaaagccgg ctatctgaaa accaaggaag aactgcttgc
     7081 ggaattggct tgccttacag cggaaatggc tgccctaaaa aagcccgatg ccttaatcca
     7141 tgggaaagaa gtgcggcaga aagaacgcaa ctcgtcgcag ggttaaggca atgccatccg
     7201 ttgaactgct gttggagatt gtcggactgc cacgcggcac cttctattac caattggccg
     7261 tccaatcggc agaagacaaa tatgccgatt tgaaacggca tatccatgat gagacctttg
     7321 caaaattccc caaaatcccc taaat
//
LOCUS       DAWHXS010000096         7322 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWHXS010000096 DAWHXS010000000
VERSION     DAWHXS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.096"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            21..653
                     /locus_tag="WIE22_001661"
     CDS             21..653
                     /locus_tag="WIE22_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ4127717.1"
                     /translation="MSAALPCAYAAGLPQSAVLHYSGSYGIPATMTFERSGNAYKIVS
                     TIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGSVTYGKAGESKTEQ
                     SPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTGKYSIGGVETEVVK
                     YRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQINGQSAKP"
     gene            771..1193
                     /locus_tag="WIE22_001662"
     CDS             771..1193
                     /locus_tag="WIE22_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ4127718.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            1762..2478
                     /locus_tag="WIE22_001663"
     CDS             1762..2478
                     /locus_tag="WIE22_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ4127719.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            2627..3529
                     /locus_tag="WIE22_001664"
     CDS             2627..3529
                     /locus_tag="WIE22_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ4127720.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            3572..4345
                     /locus_tag="WIE22_001665"
     CDS             3572..4345
                     /locus_tag="WIE22_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ4127721.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            4457..6145
                     /locus_tag="WIE22_001666"
     CDS             4457..6145
                     /locus_tag="WIE22_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ4127722.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVLELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            6238..7194
                     /gene="gshB"
                     /locus_tag="WIE22_001667"
     CDS             6238..7194
                     /gene="gshB"
                     /locus_tag="WIE22_001667"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ4127723.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELSERDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1736 a         1947 c         1999 g         1640 t
ORIGIN      
        1 atatattttc cgccgccatt ttgtccgccg ccctgccgtg cgcgtatgcg gcagggctgc
       61 cccaatccgc cgtgctgcac tattccggca gctacggcat tcccgccacg atgacatttg
      121 aacgcagcgg caatgcttac aaaatcgttt cgacgattaa agtgccgcta tacaatatcc
      181 gtttcgagtc cggcggtacg gttgtcggca ataccctgca ccctacctac tatagagaca
      241 tacgcagggg caaactgtat gcggaagcca aattcgccga cggcagcgta acttacggca
      301 aagcgggcga gagcaaaacc gagcaaagcc ccaaggctat ggatttgttc acgcttgcct
      361 ggcagttggc ggcaaatgac gcgaaactcc ccccggggct gaaaatcacc aacggcaaaa
      421 aactttattc cgtcggcggt ttgaataagg cgggtacagg aaaatacagc ataggcggcg
      481 tggaaaccga agtcgtcaaa tatcgggtgc ggcgcggcga cgatgcggta atgtatttct
      541 tcgcaccgtc cctgaacaat attccggcac aaatcggcta taccgacgac ggcaaaacct
      601 atacgctgaa actcaaatcg gtgcagatca atggccagtc cgccaaaccg taaatcgatt
      661 ttcaggcgtt tttttgccgt tttgtccata tttctgcata agccgcttga aattgtttac
      721 aataacgcca tatgcaaagc ggttttaacg ctattttcag gaatgacacc atgcaggtta
      781 catcaaaatg gatagacgga atgtgctttg tcggaacaac ggaaggcggg cacagcgtcg
      841 ttatggaggg gtcggcggca gaaggtaagg ctaagcgcgg gcccagccct ttggaaatgc
      901 tgctgttggg cgtggcgggc tgttcgagca tcgatgtggt gatgattgcc gaaaaacagc
      961 gtcagaaagt gactgactgc cgtgcgacgg ttacggcgaa acgggcggac gatgcgccgc
     1021 gcgtgtttac cgaaatccac atccatttca aagtattcgg gcatgatttg aaagaatcgg
     1081 ccattgagcg cgccgttcag atgtctgccg aaaaatactg ttcggcttcg attatgttgg
     1141 gcaaagcggc aaagattacc cacagttttg aaattgccgg ggcagataaa taggcatcgg
     1201 gcggcattat ggggaaacgg ctgatgccgt ctgaagggaa aggtgcggca ggcaatccgg
     1261 aggcgcataa aaaagcagat atattcggac tgcacctccc gaatatatct gcctgctgtt
     1321 tcctctttat tcagccttta taatacttgg acttgtcggg gtattgtgca gacttgattc
     1381 cggattgtca acaattttcg gtcaaatttt aaatgccgcg ttttaaaatg atacccgcct
     1441 gcttctgcgg gcgggcggga ggcgagaatc ccgttatttt aagccatata ttaatttatt
     1501 gaattatagt ggattaacaa aaaccagtac ggcgttgcct cgccttggct caaagagaac
     1561 gattctctaa ggtgctgaag caccaagtga atcggttccg tactatttgt actgtctgcg
     1621 gcttcgtcgc cttgtcctga tttttgttaa tccactataa aataaatttg tgtaatactt
     1681 tgatttcccc gacacgattt gaagcacagc cgccttaaaa catcttaaaa aatttgaaag
     1741 cgtaaaaata cggaaaacat catgaacttt gaaaacgacg acattatcca tgcgccgacc
     1801 acgtcttcct tgattctcga agagcggcac gattcggagc tgttccgtgt ttacgccctg
     1861 attttggacg gcattaccga tcaggtgctg ctgcccggca aaaagctgac cgaatccgaa
     1921 ctttgccgcc agatggtgtg ttcgcgcaac accgtccgcg gcgcgctgtc gcttttggcg
     1981 cacgacaaga ttgtcgattt gcaacccaac aggggcgcgt tcgtccacgt tcccgatttg
     2041 aaagaaatgc aggatgtgtt caatgcgcgc atcgaaatgg agacgatgat tttgaatatc
     2101 ctcgcagatt tgccggattt ggaaacgcgc ctcaagccgc tttatgcgat gatacggcgc
     2161 gaagaagagg cctccggcag gggagaccgc gttggctgga accgcctgtc caatgccttc
     2221 cacgtcgaac tggcgcgcct ggtgggcaac gatgtgctgt tcgacattat gaacacgctg
     2281 tgcgcgcggt cttccctgat tgtcgccgtg gcgggcgtgc atcgcgagga aaaacacgcc
     2341 atcaatacgc atacgcattc cgaacaccgc gaaatcctcg acctgctgct ggcgggcagg
     2401 cgcaaccgcg tggtcaaaat cctgcgccgc catttgggca actgtatgga gcgtttggaa
     2461 aagactttgg aagattgaat gccggagcgg ataagccgcc tgaaccttca ggcggttttt
     2521 taatggcgaa cctgatgccg tctgaaatat ggatgcgggt atctgcaatt ttcagacggc
     2581 aatttttaaa ccgcatatat catgcggcaa taaaggaggt caggagatgg acagcctgat
     2641 gacgttgctt tcggtattga taccgatgtt tgccggattt tttatccgtg tgcccaagcc
     2701 ttacctgccc gctttggata aggtgctatc ggtcttggtg tatgctgtgc tgctgctgat
     2761 cggcgtctcg ttgtcgcgcg tggaggattt gggttcgcgg ttggacgata tggcgttgac
     2821 ggttctgtgg ctgtttgttt gtacggtcgg gacgaacctg cttgctttgg cagtgttggg
     2881 aaagttattc ccgtggcgga taaaggggaa agggaagggc gtttcggtcg gtgtgtcggg
     2941 cagtgtgggg cagctcggat gcgtgctgct cggatttgca tccggcaaac tgatgcgcga
     3001 tatttggatg ccgtctgaaa acgcgggtat gtattgtctg atgctgctgg tgctcctcat
     3061 cggcgtacag ctcaaaagca gcggcgtatc gttgcggcag gttttggtca accgcagggg
     3121 tattcggttg tcggtctggt ttatgctttc atctctttca ggcgggctgc tgtttgccgc
     3181 atcggcagac ggtgtgtcgt gggtgaaagg tttggcgatg gcttccggct tcggttggta
     3241 ttccctctcg ggtttggtga tgaccgaggc ttacggcgcg gtatggggca gtatcgcgct
     3301 tttgaacgat ttggcacgag agctgttcgc gctggcattt attccgctgc tgatgaagcg
     3361 ttttcccgat gcggcagtgg gggtcggcgg cgcgaccagt atggatttca cattgcccgt
     3421 gattcggggt gcgggcggct tggaagccgt accggtagcg gtcagcttcg gcgtggtggt
     3481 caatatcgcc gctccgtttc tgatggtggt gttttccgct ttgggctgaa cgcggtaaaa
     3541 tcggcatccc gatgcaagga ggcagaaaac gatgaaaccg aaaatccaaa ggcacggaga
     3601 gattttaagc cttgtccgcc ggcatcagtt tatgtcggtg gacgagcttg ccgccgcatt
     3661 ggacgttacc ccgcagacga tacgccgcga catccgcgaa ttggaggatg ctggcagcct
     3721 gaaacgccac cacggcggcg catcctcggg cggaaacttg ccggaggggc tgcctgccag
     3781 ccgccaaatt caatgtcaaa acgaaaaaaa cgccattgcc cggctgattg cggaacatat
     3841 tcccgacgag tcatcgctgt ttgtcagtat cggcacgacg atggaagccg tggcatcgga
     3901 gctggtgaga cggcgcggca gcctgcggat tattaccaac aatatccacg tcgcctccat
     3961 cgtttcggcg cggacggatt acacggtcat catcacttcc ggagtcgtcc gccctttgga
     4021 cggcggcatt accggcgtgg caaccgtcga ttttatcaac cagttcaaag tcgattatgc
     4081 cgtgatgagt acgcacggcg tggaaagcga cggttcgctt ttggacgacg actacaaaga
     4141 agtcagcgtg atgcaggcga tgattgccaa cgcgcgcgtc cgcttcctcg gcgtggacca
     4201 cagtaaattc cgcagcaacg cgctggtcag gctcggcgac attacggcgt ttgacaaagt
     4261 atttaccgac cggctgcccg ataccgcgat gcagaagatg ctgaaagagg cgggggtgga
     4321 atgcctgatt gccgatgccg tctgaacgct atgtcaaagc gcgcaagtcg ggtacaataa
     4381 acacatcatc aaaccgcttc agacggcata aggaaccgcc ccaatgccgt ctgaagccat
     4441 ctgttcaaag aaaactatgc tcaataaaga ccaatttgcg gacaaccatt ttatccgcac
     4501 cttcatcgaa gaagacctca aaagcggcaa acacgaaacc atccaaaccc gttttccgcc
     4561 ggagcccaac ggctacctgc acatcggaca cgccaaatcc atctgcctga acttcggttt
     4621 ggcgtatatt tacgacggct tgtgcaacct gcgttttgac gataccaacc ccgaaaaaga
     4681 aaacgacgaa tacgtcaacg ccatcaaaga agacgttgag tggctcggtt tccattgggc
     4741 gggcgagccg cgtttcgctt ccaactattt cgaccagctt tatgactacg ccgtcggttt
     4801 ggttaaagac ggcaaagcgt atgtcgatga tttgacgcca gaagaaatgc gcgaataccg
     4861 cggtacgctg accgaagcag gcaaaaacag cccttatcgc gaccgcagta tcgaagaaaa
     4921 cctcgacctg ttcacgcgca tgaaaaacgg tgagttcccc gacggcagca aaaccctgcg
     4981 cctgaaaatc gacatggcat ccggcaacat caatatgcgc gatccggtca tctaccgcat
     5041 ccgccgcgcc catcaccaca acaccggcga caaatggtgc atctatccga tgtacgacta
     5101 cacgcattgt atttccgatg ccatcgaagg catcacgcat tccttgtgta cgcttgagtt
     5161 tgaagcccat cgtccgctgt atgactgggt gttggacaac atccccgcgc cgcacgccac
     5221 acgtccgcgc caatacgagt tttcacgctt ggagcttttg tacaccatta cctccaaacg
     5281 gaaattgaat cagttggttg tggaaaaaca cgtttccggc tgggacgatc cgcgtatgcc
     5341 gaccatttcc ggtatgcgcc gacgcggcta cacgcccgaa gggctgcgcc tgtttgccaa
     5401 acgcgccggt atttccaaat ctgaaaacat cgtcgatatg agcgtgttgg aaggcgcgat
     5461 tcgcgaagag ttggaaaatt ccgccccacg cctgatggcg gtgttgaacc cgctcaaagt
     5521 aaccctgacc aactttgaaa ccggcagaac ccaaagccgc cgtgccgcgt tccatccgaa
     5581 ccacgaagaa atgggcgatc gcgaagtacc tgtttcacaa accatttaca tcgaagccga
     5641 cgactttgcc gaaaatccgc ccaaaggctt caaacgcctg attcccggcg gcgaagtacg
     5701 cttgcgccac ggctatgtca tcaagtgcga tgaagtcgtc aaagacgaag caggcaatgt
     5761 gcttgaactc aaatgcagca tcgaccacga caccttgggc aaaaatccag aaggccgtaa
     5821 agttaaaggc gtgattcatt gggtttccgc cgaacacgcc gccgaaatca aagtccgtct
     5881 gtacgaccgc ctctttaccg tcgagcgtcc cgatgccgtg cgcggcgaag acggcgaata
     5941 cctgccgttc accgatttcc tcaatccgga atccgtcaag gaaatcaccg cctacgccga
     6001 acctgccgcc aaagatttgc cggcagaaag ccgttggcag ttcgagcgta tcggctactt
     6061 cgtaaccgac cgtaaagacc acggcaaaga cacgccggtg tttaaccgca cggtcacgct
     6121 gaaagattct tggcagccta agtaaaaccc catccttgcc gtctgaatat tgttcggacg
     6181 gcatttcccc tttgcccgcg aaatgcggca cattcggcac accggaaagg aaatatgatg
     6241 aaagtcctct ttatcgccga cccgatggca agtttcaaaa cctacaaaga caccacctac
     6301 gccatgatgc gcgagatggc aaaacgcggc tggcggctgt ttcatacctt gagcagcgga
     6361 ttgtcggtta aaaacggtct ggtggttgct gatgcgtctg cttttgagtt tctcggcgca
     6421 caagacgacc acgacaaaaa ctggtttgcc gccgccgaca aagttcagac ggcattgaaa
     6481 gcatttgatg ccgtgattat gcgtaccgat ccgccgttcg atatgcaata cctctacgcc
     6541 acacagcttc tgacgctggc ggagcagcag ggcgcgaagg tctttaacag cggacagtcg
     6601 atgcgtgact tcaatgaaaa actggcgatt ttgaacttca gccgctttac cgcgcccacg
     6661 ctggtaacga cccgttccgc cgatgtccgc gcatttttga aagaacacgg cgacatcatc
     6721 attaaaccgc tcgacggcat gggcggtatg ggcatcttcc gcctgaccga aaaagacccc
     6781 aacatcggca gcatcctcga aaccctgatg cagcttgatt cccgcaccat tatggcgcaa
     6841 cgctacattc ccgaaatcgt acacggtgac aaacgcatcc tgattatcgg cggcgaagtc
     6901 gtcccctatg ctttggcgcg tattcctcaa aacggcgaaa cacgcggcaa ccttgcggca
     6961 ggcgggcgcg gcgtggcgca ggaattgagc gaacgcgacc gcgaaattgc cgaaacgctc
     7021 gcccccgaac tcaaacggcg cggcatcctg ctggccggtt tggacgttat cggcagcaac
     7081 ctgaccgaag tcaacgtaac cagcccgacc ggattccaag aaattatgaa acaaaaaggc
     7141 ttcgatgtgg cggcaatgtt tgccgatgcc gttgccgcgt ggtcggtacg ttaaaccgat
     7201 gccgtctgaa aggcttttgc ttcacaaccg cctggtttga tcgggcaggc agggttttcc
     7261 ctcagtcggc agagatacgc ttaataccgt acggacaccc cgccgccccg ttttcagacg
     7321 gc
//
LOCUS       DAWHXS010000097         7246 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWHXS010000097 DAWHXS010000000
VERSION     DAWHXS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.097"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="WIE22_001668"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WIE22_001668"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WIE22_001669"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WIE22_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ4127724.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="WIE22_001670"
     CDS             complement(1737..2630)
                     /locus_tag="WIE22_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ4127725.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSSDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="WIE22_001671"
     CDS             2972..3421
                     /locus_tag="WIE22_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ4127726.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="WIE22_001672"
     CDS             3495..3803
                     /locus_tag="WIE22_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127727.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3920..4762
                     /locus_tag="WIE22_001673"
     CDS             3920..4762
                     /locus_tag="WIE22_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ4127728.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4752..5498
                     /locus_tag="WIE22_001674"
     CDS             4752..5498
                     /locus_tag="WIE22_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ4127729.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLST"
     gene            5520..6107
                     /locus_tag="WIE22_001675"
     CDS             5520..6107
                     /locus_tag="WIE22_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ4127730.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6113..6292
                     /locus_tag="WIE22_001676"
     CDS             6113..6292
                     /locus_tag="WIE22_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ4127731.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            6524..6964
                     /locus_tag="WIE22_001677"
     CDS             6524..6964
                     /locus_tag="WIE22_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127732.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1852 a         1930 c         1636 g         1828 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcgtgtcgga tgcacaccgg acgggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttaccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acagcctaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aaatcgccat ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtacg caagtctttg atacgtgagg aatccaaaac atacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcattg
      721 cgtttgcgca tttccaattc atacagtttg gacttcaaca tttccatcgc ggcggctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atgcgcacgg cggagtcggt tttattgatg tgctgaccgc ccgcacccga tgcgcgatag
      901 gtgtcgatgc gcaaatcggc ggggttgatt tcgatttcga tggaatcgtc gatttcggga
      961 tagacgaaca cggaggcaaa cgaggtatgg cgtttgttgt tcgagtcaaa cggagagtaa
     1021 cgcaccaagc ggtgaacacc tgtttccgtg cgcagcaagc cgtaggcata ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatgc ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc tgcgcctgcg gtaatgtcga taaagcagtt gttcggatcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgaagcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgacaac ttcttccaat cggtctttct taccctgata atccatataa
     1561 actcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tggaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaaccgga tggcgcgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgag ataagagaag aagccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atctgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cggacgacat acccgatgca aaaaggctgc cgactatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaataccg ttttctttca aaaccttaat aatacctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcagcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggcggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcctttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttccga aatcaacgat cagtttccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tactgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccggcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccccc cccttcaaac caagccgcgt ttttcagtaa
     3841 aatgcggctt ttttaacggc aaacaaagat tttttaacaa gcacatcatt cttttgtgcc
     3901 atccgaaccg ggtaaaaata tgattcaaca cgcaggcttg ggctaccgcc gcgacttggc
     3961 ggaagacttt ctctcgcttt ccgaaaacag cccgatatgc tttatcgaag ccgcaccgga
     4021 aaactggctg aaaatgggcg gctgggcgcg caaacagttt gaccgtgtgg cggaacggct
     4081 gccgctggcg ttgcacggat tgtctatgtc gctgggcggg caagcaccgc tggatactga
     4141 tttgatagac ggcatcaaag aaatgatgcg ccgttacgat tgcacgtttt tctccgacca
     4201 tttgagctac tgccacgacg gcggtcatct ttacgatttg ttgccgctgc ccttcaccga
     4261 ggaaatggtg catcatacgg cgcggcgtat ccgtgaagtg caagaccgtt tgggctgccg
     4321 catcgccgtg gaaaacacgt cctactatct gcattccccg cttgccgaga tgaacgaggt
     4381 cgagttcctc aacgccgtcg cacgtgaggc cgattgcggc attcatctgg atgtgaacaa
     4441 tatctacgtc aacgccgtca atcacggcct gttgtcgccg gaggcttttt tgaaaaacgt
     4501 tgacgcagat cgcgtgtgct atatccatat tgccggacat gacgtggaaa cgccggaatt
     4561 gttgattgat acacatggcg cggcagtttt gccgactgtt tgggacttgc tcgaacttgc
     4621 ctatgccaag ctgccgacga ttccgcccac cctgttggaa cgcgatttta atttcccgcc
     4681 ttttgccgaa ctcgaagccg aagtcgccaa aatcgccgat tatcaaacgc gtgccggaaa
     4741 ggaataccgc cgtgcagcct gaaacctccg cccaatacca gcaccgtttc gcccaagcca
     4801 tacgcggggg cgaagccgca gacggtctgc cgcaagaccg actgaacgtc tatatccgcc
     4861 tgatacgcaa caatatccac agctttatcg accgctgcta taccgaaacg cggcaatact
     4921 ttgacagcga agaatggagc cgtctgaaag aaggtttcgt ccgcgacgcg cacgcccaaa
     4981 cgccctattt tcaagaaatc cccggcgagt tcctacaata ttgccaaagc ctgccgcttt
     5041 cagacggcat tttggcactg atggattttg aatataccca attgctggca gaagttgctc
     5101 aaattccgga tattcccgac attcattatt caaatgacag caaatacaca ccctcccctg
     5161 cggcctttat ccggcaatat cgatatgatg ttaccgatga tttgcatgaa gcggaaacag
     5221 ccttgttaat atggcgaaac gccgaagatg atgtgatgta ccaaacattg gacggcttcg
     5281 atatgatgct gctagaaata atgggttcct ccgcgctttc gtttgacacc ctcgcccaaa
     5341 cccttgtcga atttatgcct gaggacgata attggaaaaa tattttgctt gggaaatggt
     5401 caggctggac tgaacaaagg attatcatcc cctccttgtc cgccatatcc gaaaatatgg
     5461 aaggcaattc cccgggccaa aaccatctat ccacataaaa ttaccttgtt cccgatacta
     5521 tgccgctacc cgacctgacc gatgccgaat taatagagtc gcgtaaactg cttctgcatt
     5581 ttgcgcggct tcagttgccc gaccaccctg atttggctga agatttagtg caggaaacat
     5641 tgctgtccgc atacagcgca ggcgacagtt ttcaaggcag ggcacttgtc aacagctggc
     5701 tttttgccat attgaaaaac aaaattattg acgcattacg tcaaatcgga aggcagagga
     5761 aagtctttac cacactggat gacgagctac tggatgaagc atttgaaagc catttttccc
     5821 aaaacgggca ttggacgccg gaagggcagc cgcaacattg gaacactccg gaaaaatcat
     5881 taaacaacaa cgaattccaa aaaattctgc aaagctgcct atacaacttg cctgaaaaca
     5941 ccgcacgggt atttaccctg aaggaaatac tcggtttttc atccgacgaa atacaacaaa
     6001 tgtgcggtat cagcacgtcc aactaccaca ccattatgca ccgcgcccga gaatcattgc
     6061 gccaatgcct gcaaatcaaa tggttcaacc aagaaaaccc gaagtaaacg ttatgaaaaa
     6121 atgccgcgat atcgccctgc ttctttccaa acatcaggac cgggaaacca ccccgggcga
     6181 gaagatttcc atatacacac acctgctgtt ctgcccacat tgccgagaat ataaaagaca
     6241 gcttcaaacc atcaaaatat cactggcaaa aacaaccaga acttcaaaat aaatgccgtc
     6301 tgaaaaggct tcagacggca taagctgacg gaaaaaaatc aaaccgattt actgttatcg
     6361 gcagttcatc cataatacac gcttcaaaag cagcatattc ctccatacgg aatatataaa
     6421 tacgcaaaac acgaaggctg catcaatttg ctttatttgc cttatttcac agacggcgct
     6481 acccctcccg cccaacccgt tctttctgaa tgagcagatt tcaatgatta aggaaaccct
     6541 aatgcgccca atcttcctat ctttcgtttt actccctatt ttgataaccg cctgcagcac
     6601 accggacaag tctgcccgat gggaaaatat cggcacaatc tcaaacggca atattcatac
     6661 atatatcaat aaagacagcg tgagaaaaaa cggaaatctg atgattttcc aagataaaaa
     6721 agttgttacc aatctaaaac aagaacgttt tgccaacacc cccgcataca agactgccat
     6781 tgccgagtgg gaaatccact gcaacaacaa aacataccgc ttaagttcgc tacagttgtt
     6841 tgatacaaaa aacacggaaa tttccacaca aaactacaca gcctcttccc tccgcccgat
     6901 gagcatcctg tccgggacat taaccgaaaa acaatatgaa accgtatgcg gaaaaaaact
     6961 ctgattgcaa cttatacaca aacttaccca caaaccttat cataaaaatg ccgtctgaaa
     7021 tactgaaata tcagcatttc ggacggcatt ttgccatttc tccgaaaagt tatcctcgaa
     7081 gttatccaca ttattttttt aaaccagctt ccatccgaaa tataaacctg ctatacaatt
     7141 tcattatcaa aacgtaaatt gttccattga tacacaaaac tgcttacccc cataattttg
     7201 ataaagcatt tcttacattc ccggctccgt cccgtaatca acacag
//
LOCUS       DAWHXS010000098         7116 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWHXS010000098 DAWHXS010000000
VERSION     DAWHXS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.098"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..101
                     /locus_tag="WIE22_001678"
     CDS             <1..101
                     /locus_tag="WIE22_001678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEZ4127733.1"
                     /translation="TFACVLGALVASEHGATPDISPERIERFMNPV"
     gene            complement(161..718)
                     /locus_tag="WIE22_001679"
     CDS             complement(161..718)
                     /locus_tag="WIE22_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ4127734.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="WIE22_001680"
     CDS             complement(794..2428)
                     /locus_tag="WIE22_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HEZ4127735.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKHSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2706..3812)
                     /gene="serC"
                     /locus_tag="WIE22_001681"
     CDS             complement(2706..3812)
                     /gene="serC"
                     /locus_tag="WIE22_001681"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ4127736.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGATVVIIRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTEDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4177..4608
                     /gene="rimP"
                     /locus_tag="WIE22_001682"
     CDS             4177..4608
                     /gene="rimP"
                     /locus_tag="WIE22_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ4127737.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4636..6138
                     /gene="nusA"
                     /locus_tag="WIE22_001683"
     CDS             4636..6138
                     /gene="nusA"
                     /locus_tag="WIE22_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ4127738.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6151..>7116
                     /locus_tag="WIE22_001684"
     CDS             6151..>7116
                     /locus_tag="WIE22_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEZ4127739.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDTAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNR"
BASE COUNT         1910 a         2056 c         1769 g         1381 t
ORIGIN      
        1 agacctttgc ctgcgtactt ggtgcgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttcccccga acgcatcgag cgtttcatga atccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg catttcgctt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacagg gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt acaccaatga ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttc aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttggct attggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgagg cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aggtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtacc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccggc cggccggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aatgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaagctcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcg gcagccgtag
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 gggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggaa ggcgacgcag gctgtcggat acacatctcg gcacaaacag
     2581 catcacgccc ggattgaaaa ggacggatag ccgccggcag cacaatctta ccacctccaa
     2641 aacgccgccg gagaacgccg aaacagccga tgccgtctga agccgcttca gacaacatcg
     2701 ggacatcaac cgtaacgccg ttggaaatcg cgcataaaat ctgccaaagc ccgcacgcct
     2761 tcaagcggca tcgcattata aatgctggca cgcataccgc cgacggtttt atagccctta
     2821 agcaggcaca agccctgcaa ttcggcttcc agcacaaaac ggcggtcaag ctcctcatcc
     2881 tccgtttgga acacgacatt cattttagaa cgcgcattcg gacggatacg gttgatatag
     2941 aaaccgccgc tgccatctat cgtctcatac aaggtttgcg ccttcagccg attgaccgct
     3001 tcaatttttt tcacaccgcc ctgcgcctgc agccagcgga acaccagccc cgacatataa
     3061 atcgcgtaag ttgacggcgt gttgtacata ccgtcgcggt tgatgtgcga acggtagttg
     3121 aacacatcgg gaatatcgtt cggacaacgc tcgagcaaat cctcgcggat aatgaccacg
     3181 gtcgcaccgg ccgggccgat gtttttctgt gcgcctgcgt aaatcagtcc gtagtcggca
     3241 acatcaaact cgcgcgacaa aatctcgctg gacatatcgc acaccagcgg cggcatgcct
     3301 tctgaaaggc gcggcacttc acggtattgc agcccgttga ccgtttcatt gacggcaaaa
     3361 tggacaaacg ccgaatcggg tgcaacatcc cacgtttcca caggcggcag gtcgagatag
     3421 tcgaactgct cgccgccatg cgccgccaaa cggatttccg tatcggtcaa acggctcatc
     3481 tgttcataag cgatacggct ccagttgccc gttaccaccg cgtcggcagt gcggaaaccg
     3541 tgtgccagat tcatggctgc catattaaat tgggttgttg ctccgccctg cagaaacaat
     3601 atcttatagt tgtcaggcac tttcaaaagc tgcctcaaat cctgttccgc atgatgcagg
     3661 atgctcaaaa acatttccga acggtggctc atcgccatca cgggaaaacc cgtaccgttg
     3721 tagtccaaca tttcctgccg cgccgtttcc aacacggctt cgggcaatac ggcagggccg
     3781 gcggaaaaat tgtaaatcgg ataaagagac atgatgcagc cttgattctg aacaataacc
     3841 cgcctgattt taggcttgca gcaggctttg tgcaaggatg ggaaatacct gtcctccgcc
     3901 ccgcttcatg ccgcccgaac acggaaaaca acatcaatat attgatttac aaatataaaa
     3961 atcatgctag cgacaaatag atacatttgt tttgtcaaca atattcacga tttcccatta
     4021 caaacctccc ttacacccgc ttttttccgt cccaaaaaca caaaataaat caacactttc
     4081 atttctccgc aaaagcggtt ataatcacgc cgatttttca actttgacga aaaatggcca
     4141 cggggccatt tttttattat gtacataggg agcagcatgg atatccaaac catcctcgaa
     4201 aaaaccctgc ccggcctggg ctacgaactg gtcgatttcg aactgaccgc gcaaggaaca
     4261 ttgcgcgtgt tcatcgacaa agaaggcggc attaccgtcg aagactgcgc aaccgtcagc
     4321 aaccacttga gccgcgtctt catggttgaa gacatcgact acaaaaacct ggaaatttcc
     4381 agccccggac tcgaccgccc cttgaaaaaa gccgccgact tcgtgcgctt tgccggtcag
     4441 aatgccaaaa tcaaaacccg cctgccgata gacggtcaaa aaaactttat cggtaaaatc
     4501 gaaggctgcg aaaacgacac tgttaccgtt tccttcgacg gcaaaaccgt acaaatcgaa
     4561 ttgggcaaca tcgacaaagc ccgcctgcgc cccgaattca aattctaaaa cacaacaata
     4621 ttggagatgt tcaaaatgag tcgtgaaatg ttacagctgg cagaagcact ggcaagcgaa
     4681 aaaaacgttg atgccgaagt cgtcttccaa gcactggaat tcgccctgtc taccgccgcc
     4741 aagaaaaagg cagaccgcga acacatggat gtccgcgtac aaatcaaccg cgacaccggc
     4801 gaataccaaa ccttccgccg ctggctgatt gtcgccgatg aagactatac ctatcccgat
     4861 gtcgaaaaaa ccatcgagga aatccaagag gaaattcccg gcactaccat ccaaatcggc
     4921 gaatactacg aagagcagct gcccaacgaa ggcttcggcc gccaagccgc gcaaaccgcc
     4981 aaacaaatca tcctgcaacg catccgcgat gccgagcgcg agcagaatct gaacgagttt
     5041 ctcgccgtca aagaagacat cgtgtccggc acggtcaaac gcgtcgaacg ccacggcatc
     5101 atcgtcgaag tcgttgccgg caaactggac gcgctgattc cgcgcgacca aatgattccg
     5161 cgcgaaaact tccgcagcgg cgaccgcatc cgcgccctct tcctgcgcgt cgaagaaatc
     5221 ggcaacaccg gccgcaaaca agtcatcctg agccgtactt ccggcgattt cctcgtcaaa
     5281 ctgtacgcca atgaagtacc tgaaattgca gacggcatgc ttgaaatccg cgctgtcgcc
     5341 cgcgacccgg gccagcgtgc caaagtcgcc gtcaaagcca acgaccagcg catcgatccg
     5401 caaggcacct gtatcggcgt tcgcggttcg cgtgtcaatg ccgtcagcaa cgaattgtcc
     5461 ggcgagcgca tcgacgttgt cctttggtcg cccgagcctg cccaattcgt catgagcgcg
     5521 ctctcacccg ccgaagtcag ccgcatcgtc atcgacgaag acaaacacgc cgtcgatgtc
     5581 atcgttgccg aagaccagct cgcgctcgcc atcggacgcg gcggtcaaaa cgtgcgcctt
     5641 gcttccgacc tgaccggctg gcagctcaac atcatgactt ccgccgaggc agacgaacgc
     5701 aatgcggcag aagatgccgc catccgccgc ctgtttatgg atcacttgaa cgtggacgaa
     5761 gaaaccgccg acgtactggt tcaggaaggt tttgcaacct tggaagaagt cgcctatgtt
     5821 cctgccgccg aactgcttgc cattgaagga tttgacgaag aaatcgtcga tatgctccgc
     5881 aaccgcgccc gcgatgccat cctgaccatg gcgattgccg ccgaagaaaa actgggcgaa
     5941 gtatccgacg atatgcgcaa cctcgaaggc gtagatgccg atatgctccg cagccttgcc
     6001 gaagcaggca ttaccacccg cgacgacttg gcagagcttg ccgtggacga actgattgaa
     6061 attaccggtg taaacgaaga aaccgcaaaa gccgtcatcc tgaccgctcg cgaacactgg
     6121 tttaccgaag acaaataaaa gggggtacag atgagtaaca caaccgtaga acaatttgcc
     6181 gccgagctga aacgccccgt cgaagacctg ttgaaacagt tgaaagaagc cggcgtcagc
     6241 aaaaacagcg gcagcgattc cctgacgctg gacgacaaac agcttctgaa cgcctacctg
     6301 accaagaaaa acggcagcaa cggcggcacc atcagcatcc gccgcaccaa aaccgaagtc
     6361 agcaccgttg acggcgtaaa agtcgaaaca cgcaaacgcg gacgcactgt caacattcct
     6421 tctgccgaag aattggcagc acaggtaaaa gccgcccaaa cccaagccgc acctgtccgg
     6481 ccggagcaga cggcagaaga cacggcaaaa gcccgagccg aagctgccgc acgtgcagaa
     6541 gcccgtgcca aggcagaagc ggaagcggca aaactgaaag cggcaaaagc aggcaacaaa
     6601 gccaaacctg ccgcgcagaa acccaccgaa gcaaaagccg aaaccgcacc tgttgcggcg
     6661 gaaaccaaac ccgccgaaga aagcaaagcg gaaaaagccc aagccgacaa aatgccgtct
     6721 aaaaaacccg ccgagcccaa agaaaaagcc gccaagccga aacacgagcg aaacggcaaa
     6781 ggcaaagatg ccaaaaaacc agcgaaacct gccgcacctg ccgtgccgca acccgtggtc
     6841 agcgcagaag aacaggcgca acgcgatgaa gaagcacgcc gtgccgccgc acttcgcgcc
     6901 caccaggaag ccctgttgaa agagaaacag gaacgccagg cacgccgcga agccatgaaa
     6961 caacaggcag aacaacaggc aaaaaccgca caggaagcca aaaccggcag acagcgtccc
     7021 gccaaacctg ccgagaaacc gcaggcagcc gcgccggccg tcgaaaacaa gcctgtcaat
     7081 ccggcaaaag cgaaaaaaga agaccgccgc aaccgc
//
LOCUS       DAWHXS010000099         7022 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWHXS010000099 DAWHXS010000000
VERSION     DAWHXS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.099"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WIE22_001685"
     CDS             complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="WIE22_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ4127740.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="WIE22_001686"
     CDS             1555..1710
                     /locus_tag="WIE22_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127741.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="WIE22_001687"
     CDS             1707..2138
                     /locus_tag="WIE22_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ4127742.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2272..6642
                     /gene="app"
                     /locus_tag="WIE22_001688"
     CDS             2272..6642
                     /gene="app"
                     /locus_tag="WIE22_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ4127743.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETQPATTAFP
                     RARRARRDLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAE
                     DRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTF
                     DDGIGNSARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGSKIRRRVLHYGIQA
                     RYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQ
                     HISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAA
                     AKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1871 a         2030 c         1701 g         1420 t
ORIGIN      
        1 accaaacgga tcggattccc gtttttatgg gataacggaa tgttcagccg gacaccgcca
       61 cgatgctttc gatccgttta ttttccgata caaaacctcg aaaaaatcac gccgagcagg
      121 tcgtccgccg taaactcgcc cgtgatttcg ccgcatgcga cctgcgccaa gcgcaagtgt
      181 tcggcaaaca gctcgatttg atggttgccg cacaatgccg ccagcgacaa ttcttcctgc
      241 gctgctttga gtgcgttgac gtgccgcgtc cgcgccaaaa acagcccttc gccctcgccc
      301 tgccatccgg cctcgcgcaa caacgtccgt ttcagcgcgt ccaagccgtc gccggttttc
      361 gccgacagcg cgatgacggt ttccgcgccc gtaccgaacc cgcctgccgc gtgtgcgtgc
      421 aaatcggatt tgctgtggat ttcgatgcgt ttcaactccg gcggcaacgc gtccaaaatc
      481 gcccgtgtct tgtcgttcac gccttcgcgc ggatcgacca acaccagcgc gacatcggct
      541 tcggatacgg ctttgcggct gcgttcgatg ccgatgcgtt cgaccacgtc gtccgtctcg
      601 cgcaaacctg ccgtatcgac aatatgcacc ggaacgccgt caatcaggat acgttccctg
      661 accgcgtcgc gcgtcgttcc ggcaatatcg gtaacaatcg ccacttcgtc gcccgccagc
      721 gcgttcaaca ggctggattt gccgacattc ggcgcgccga ccaatacgac attcagacct
      781 tcgcgcaaaa tcgcgccctg ctgcgcgttg gcaagcacat catccacggc gcggcgcaag
      841 ccgtccagtt tgccgcgtgc gtctgccgct tcgagaaaat caatgtcttc ctcgggaaaa
      901 tctaacgtcg cttcgaccag catccgcaag gtaatcaagt cttcgaccag accgtgtatc
      961 cgccgcgaaa aatcgccttt gagcgagcgc aacgccagac gcgccgccga acggctggat
     1021 gcgtcaatca aatccgccac gccttccgcc tgtgccaagt ccagtttgtc gtttaaaaac
     1081 gcacgcttcg taaactcgcc cggttcggca aggcgcgcgc ccaattccaa acagcggttc
     1141 agcagcatat ccatcactac cggcccgccg tgtccctgaa gctcgataac atcttcgccc
     1201 gtaaaacttg ccggtgcggc aaaaaaaagc aaaagcccgc tgtcgattgc ctgaccgtcc
     1261 gcgtccgtaa aatcagcata ggttgccacg cgcggcttgg gcgttttccc acacaaagcc
     1321 tctgccatcg gcagcaggtt tttccccgat atgcgtatca cgcccacgcc gccgcgcccg
     1381 ggtgcggtag cgactgccgc aatcgttgga acgttatccg acataaaacc cccgaaaatt
     1441 caaaacagcc gcgattatag caaatgccgt ctgaagtccg acggtttggc tttcagacgg
     1501 cataaaaccg caaaaatgct tgataaatcc gtccgcctga cctaatataa ccatatggaa
     1561 aaacgaaaca catacgcctt cctgctcggt ataggctcgc tgctgggtct gttccatccc
     1621 gcaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgcaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagcccgg cgtttccatt cgggaccgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgtc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt ggttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggccg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattc ttccccaagc ttgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtgt cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg aaggaagcaa tcccgatcaa catcgtttta cttataaaat tgtgaaacgg
     2701 aataattata aagcagggac taacggccat ccttatggcg gcgattatca tatgccgcgt
     2761 ttacataaat ttgtaaccga tgcagaacct gttgaaatga ccagttatat ggatgggcgg
     2821 aaatatatcg atcaaaataa ttaccctgac cgtgttcgta ttggggcagg caggcaatat
     2881 tggcgatctg atgaagatga gcccaataac cgcgaaagtt catatcatat tgcaagtgcg
     2941 tattcttggc tcgttggtgg caataccttt gcacaaaatg gatcaggtgg tggcacagtc
     3001 aacttaggta gtgaaaaaat taaacatagc ccatatggtt ttttaccaac aggaggctca
     3061 tttggcgaca gtggctcacc aatgtttatc tatgatgccc aaaagcaaaa gtggttaatt
     3121 aatggggtat tgcaaacggg caacccctat ataggaaaaa gcaatggctt ccagctggtt
     3181 cgtaaagatt ggttctatga tgaaatcttt gctggagata cccattcagt attctacgaa
     3241 ccacgtcaaa atgggaaata ctcttttcac gacaataata atggcacagg aaaaatcgac
     3301 gccaaacatg aacacaattc tctgcctaat agattaaaaa cacgaaccgt tcaattgttt
     3361 aatgtttctt tatccgagac agcacaagaa cctgtttatc atgctgcagg tggtgtcaac
     3421 agttatcgac ccagactgaa taatggagaa aatatttcct ttattgacga aggaaaaggc
     3481 gaattgatac ttaccagtaa catcaaccaa ggtgctggag gattatattt ccaaggagat
     3541 tttacggtct cgcctgaaaa caacgaaacg tggcaaggcg cgggcgttca tatcagtgaa
     3601 gacagtaccg ttacttggaa agtcaatggc gtggcaaacg accgcctgtc caaaatcggc
     3661 aaaggcacgc tgcacgttca agccaaaggg gaaaaccaag gctcgatcag cgtgggcgac
     3721 ggtaaagtta ttttagatca acaagcagat gaaaataata aaaaacaagc ctttagtgaa
     3781 atcggcttgg tcagcggcag gggtacggtg caactgaatg ccgataatca gttcaacccc
     3841 gacaaactct atttcggctt tcgcggcgga cgtttggatt tgaacgggca ttcactttcg
     3901 ttccaccgta ttcaaaatac cgatgaaggg gcgatgattg tcaaccacaa tcaagacaaa
     3961 gaatccaccg ttaccattac aggcaataaa gatattgcta caaccggcaa taacaacagc
     4021 ttggatagca aaaaagaaat tgcctacaac ggttggtttg gcgagaaaga tacgaccaaa
     4081 acgaacgggc ggctcaacct tgtttaccag cccgctgcag aagaccgcac cctgctgctt
     4141 tccggcggaa caaatttaaa cggcaacatc acgcaaacaa acggcaaact gtttttcagc
     4201 ggcagaccaa caccgcacgc ctacaatcat ttaaacgacc attggtcgca aaaagagggc
     4261 attcctcgcg gggaaatcgt gtgggacaac gactggatca accgcacatt taaagcggaa
     4321 aacttccaaa ttaaaggcgg acaggcggtg gtttcccgca atgttgccaa agtgaaaggc
     4381 gattggcatt tgagcaatca cgcccaagca gtttttggtg tcgcaccgca tcaaagccac
     4441 acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa aaccattacc
     4501 gacgataaag tgattgcttc attgaccaat accgacatca gcggcaatgt cagccttgcc
     4561 gatcacgctc atttaaatct cacagggctt gccacactca acggcaatct tagtgcaaat
     4621 ggcgatacac gttatacagt cagccacaac gccacccaaa acggcgacct tagcctcgtg
     4681 ggcaatgccc aagcaacatt taatcaagcc acattaaacg gcaacacatc ggcttcgggc
     4741 aatgcttcat ttaatctaag cgaccacgcc gtacaaaacg gcagtctgac gctttccggc
     4801 aacgctaagg caaacgtaag ccattccgca ctcaacggta atgtctccct agccgataag
     4861 gcagtattcc attttgaaag cagccgcttt accggacaaa tcagcggcag caaggatacg
     4921 gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt aggcaattta
     4981 aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc ggcaggggcg
     5041 caaaccggca gtgcgacaga tgcgccgcgc cgccgttcgc gccgttccct attatccgtt
     5101 acacctccgg cttcggcaga atcccatttc aacacgctga cggtaaacgg caaattgaac
     5161 ggtcagggaa cattccgctt tatgtcggaa ctcttcggct accgcagcga caaattgaag
     5221 ctggcggaaa gttccgaagg cacttacacc ttggctgtca acaataccgg caacgaacct
     5281 gcaagcctcg aacaattgac ggtagtggaa gggaaagaca acaaaccgct gtccgaaaac
     5341 cttaatttca ccctgcaaaa cgaacacgtc gatgccggcg cgtggcgtta ccaactcatc
     5401 cgcaaagacg gcgagttccg cctgcataat ccggtcaaag aacaagagct ttccgacaaa
     5461 ctcggcaagg cagaagccaa aaaacaggcg ggaaaagaca acgcgcaaag ccttgacgcg
     5521 ctgattgcgg ccgggcgcga tgccgtcgaa aagacagaaa gcgttgccga accggcccgg
     5581 caggcaggcg gggaaaatgt cggcattatg caggcggagg aagagaaaaa acgggtgcag
     5641 gcggataaag acagcgcctt ggcgaaacag cgcgaagcgg aaacccagcc ggctaccacc
     5701 gccttccccc gcgcccgccg cgcccgccgg gatttgccgc aaccgcagcc ccaaccgcaa
     5761 cctcaacccc aaccgcagcg cgacctgatc agccgttatg ccaatagcgg tttgagtgaa
     5821 ttttccgcca cgctcaacag cgttttcgcc gtacaggacg aattggaccg cgtgtttgcc
     5881 gaagaccgcc gcaacgccgt ttggacaagc ggcatccggg acaccaaaca ctaccgttcg
     5941 caagatttcc gcgcctaccg ccaacaaacc gaccttcgcc aaatcggtat gcagaaaaac
     6001 ctcggcagcg ggcgcgtcgg catcctgttt tcgcacaacc ggaccgaaaa caccttcgac
     6061 gacggcatcg gcaactcggc acggcttgcc cacggcgccg ttttcgggca atacggcatc
     6121 ggcaggttcg acatcggcat cagcacgggc gcgggtttta gcagcggcag tctttcagac
     6181 ggcatcggaa gcaaaatccg ccgccgcgtg ctgcattacg gcattcaggc acgataccgc
     6241 gccggtttcg gcggattcgg catcgaaccg cacatcggcg caacgcgcta tttcgtccaa
     6301 aaagcggatt accgctacga aaacgtcaat atcgccaccc ccggccttgc gttcaaccgc
     6361 taccgcgcgg gcattaaggc agattattca ttcaaaccgg cgcaacacat ttccatcacg
     6421 ccttatttga gcctgtccta taccgatgcc gcttcgggca aagtccgaac gcgcgtcaat
     6481 accgccgtat tggctcagga tttcggcaaa acccgcagtg cggaatgggg cgtaaacgcc
     6541 gaaatcaaag gtttcacgct gtccctccac gctgccgccg ccaaaggccc gcaactggaa
     6601 gcgcaacaca gcgcgggcat caaattaggc taccgctggt aaccgccgga tatgccgaaa
     6661 ggggtctgac gatgccaccg tgcgctgtca aacccttttt ctgccgccgc cttgtgcctg
     6721 ctccattgtc tgataaccgt caaattgatt tttagcccat gtttggtggg tcggggaaat
     6781 ctatatcttc gtccgtgccg aaatagtctg agacctttgc aaaattcctt tccctcccga
     6841 cagccgaaac ccaaacacag gttttcgtct attttcgccc caaatacctc ctaattctac
     6901 ccaaataccc ccttaatcct ccccggatac ccgataatca ggcatccggg ctgcttttta
     6961 ggcggcagcg gacgcactta acctgttggc cgctttcaac aggttcaaac acatcgcctt
     7021 ca
//
LOCUS       DAWHXS010000100         6986 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWHXS010000100 DAWHXS010000000
VERSION     DAWHXS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.100"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..218)
                     /locus_tag="WIE22_001689"
     CDS             complement(<1..218)
                     /locus_tag="WIE22_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4127744.1"
                     /translation="MASSGVNNEIKDKKLSLWAKRQDGSVKWFCGQPVTRADTDTDTA
                     VTAASDKQIDPKHLPSTCRDESTAGCTN"
     gene            complement(247..576)
                     /locus_tag="WIE22_001690"
     CDS             complement(247..576)
                     /locus_tag="WIE22_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ4127745.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLAIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(654..872)
                     /locus_tag="WIE22_001691"
     CDS             complement(654..872)
                     /locus_tag="WIE22_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ4127746.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            994..1947
                     /locus_tag="WIE22_001692"
     CDS             994..1947
                     /locus_tag="WIE22_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ4127747.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIIITNKVMISADIIAHDPQLELIAVSATGVNNVDIEAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLARYAQAFGMRVVFAEHKHAPVVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALVAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2022..4079)
                     /gene="metG"
                     /locus_tag="WIE22_001693"
     CDS             complement(2022..4079)
                     /gene="metG"
                     /locus_tag="WIE22_001693"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ4127748.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLDECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPILPKVAEN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESQYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYLTPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4194..6032)
                     /gene="glmS"
                     /locus_tag="WIE22_001694"
     CDS             complement(4194..6032)
                     /gene="glmS"
                     /locus_tag="WIE22_001694"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ4127749.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6314..6832
                     /locus_tag="WIE22_001695"
     CDS             6314..6832
                     /locus_tag="WIE22_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127750.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1466 a         1655 c         2099 g         1766 t
ORIGIN      
        1 tggtttgtgc aaccggcagt tgattcatcg cggcaggttg acggcaggtg cttggggtcg
       61 atctgcttgt cgctggcggc ggtaacggcg gtgtcggtgt cggtgtcggc gcgcgtaacc
      121 ggctgtccgc agaaccattt taccgaaccg tcttgacgct tggcccacag ggagagtttt
      181 ttgtctttga tttcgttgtt tacgccgctt gaagccatta tgtcagacgg tattgcccgg
      241 gcagctttat tcgtacactt tcagcagctc gacttcaaat atcaaagtgg cgtgcggggg
      301 aatcacgccg cccgcgccgt gtgcgccgta gcccatttcc gaagggatgg ccagcttgcg
      361 tttgccgcct tccttcatgc cgccgaagcc ttcgtcccag cctttgatga cttgtccgac
      421 accgagcgtg atggtcagcg gctggcggcg gtcgaggctg gagtcgaatt tggttccgtt
      481 ttccagccaa cctgtgtaat gcacggtaat ctctttgcct ttaactgctt cttttccgaa
      541 gccttcttgc aagtcttcaa taatcaggcc gcccatattt gtcctttcgt tgcttgttgg
      601 tcaaaacggc aagggtaaca taccgtccgt cgaagtcaaa tgccgctcaa acgtcagctg
      661 catcggtgca gctgaaacgg ctgtgtttgt tggactgttt tatttttttc gtaaaggttc
      721 catgcttttt catggaaata gaaaacgacg gtgttgatta ggggctcgac cagcgcgacc
      781 gcccccgata cgcctatact gcccgtcagt acataggtta cactgaaggc ggtgctgaaa
      841 tgcagtgcgg caaaagtcag ggttttaagc atcatcctct cccgaattgg acattgacgg
      901 aggaatgata aaggttatca taaggttgcg cggtttaaat ttgctatttg ttgttagtgt
      961 aaataaatca tttttcaaat ggggatagaa attatgaatc ataaaaagat cgttgttttg
     1021 gatgcggata ctttacccgg ccgggttttt cattttgatt ttccgcacga gcttgcggtt
     1081 tacggtacga caggtgcgga tgaaacggca gaacgggtgc gcgacgcgca tattatcatc
     1141 accaataaag tgatgatttc agcggatatc attgcacacg atccgcaact tgaattgatt
     1201 gccgtcagtg cgaccggtgt gaacaatgtc gatattgagg cggcgaaggc ggcgggcgtt
     1261 gcggtatgca atgtccgcgc ctacggaaac gaatcggttg cggaacacgc ctttatgctg
     1321 atgattgcgt taatgcggaa tttgcccgcc tatcagcgtg atgttgcggc aggattatgg
     1381 gaaaagtcgc cgtttttctg ccattatggc gcgccgattc gggatttgaa cggtaaaacg
     1441 ctggcggttt tcggacgcgg caatatagga cggacgcttg cccgatatgc acaggcattc
     1501 ggtatgaggg tagtgtttgc cgaacacaaa cacgcgcccg ttgtgcgtga aggctatgtt
     1561 tcctttgaag atgcggtaca gactgccgac gtgttgtcgc tgcactgtcc gctgaacgcc
     1621 caaactgaaa atatgatagg cgaaaacgaa ttacagcaga tgaagcccgg tgcggttttg
     1681 atcaattgcg ggcgcggtgg gctggtggat gaaaatgcgc tggttgccgc actcaaatac
     1741 gggcagattg gcggggcagg tgtcgatgtt ttgacggagg aaccgcctag gggcggcaat
     1801 cccttgctga atgcacgact tcccaatctg attgttacgc cgcataccgc gtgggcaagt
     1861 cgtgaagcgt tggacaggct gttcgatata ttgttggcga acattcacgc ctttgtgaaa
     1921 ggcgaggcgc aaaaccgcgt ggtttgaacc tgtcgggatt gcggaaaaaa atgccgtctg
     1981 aacgcctcaa gggttcagac ggcatttttt gagatttctg cttaaccgac tttgtcgccc
     2041 ggttgcgcgc ctgcatccac atccaagagc ttcagtttcc cgtctgccgt ggcggcagag
     2101 agaatcatgc cttcggatac gccgaatttt gccattttgc gcggggcgaa gttggcgacg
     2161 gcgatgacca tccttccgtt taactcggca ggggtcaggt aagacgcggc gatgccggag
     2221 aagatgatgc gtttttcaaa accgaaatcg aggtcgaatt tcaaaagttt ggtgctgcct
     2281 tcgacagctt cgcagttcaa tactttggca acgcgcatat cgattttcat aaagtcgtcg
     2341 aagcttgcct gttcggcaac tttttcgtat tgactttctt cggcggcagg tgcggctgcg
     2401 gcggcgatgc tttgtttgtt ggcttcgatt aaatcgtcca cttgtttttg ctccactcgt
     2461 tgcatgagat gttcgtattt gttgatggcg tgttcgccca aggtttcgcg tgtgtttgcc
     2521 caagtgatgg cttccaaatt caggaatttg gcggcgtttt cggccacttt cggcaagatg
     2581 ggggcaaggt aggcggtcag catggtaaag gcgttgatga gttcgctgca tacttcgtgc
     2641 aggcgttcgt cttggccttc ttgtttcgcc agttcccaag gtttgttggc atcgacgtat
     2701 tcgttgacaa tgtctgccaa ggccatgatg tcgcgcaggg cgcgggcgta ttcgcggttt
     2761 tcgtattgct cggcgatggt gtcgctttcg gcggcaagtt ttgccagcaa tgcgctgcct
     2821 gaaacgtctt tcagacggcc ttcaaagcgt ttggcgatga aacctgatgc gcgggcggcg
     2881 atgttgacgt atttgccgac gaggtcgctg tttacgcgcg aaataaagtc ttgcaggttc
     2941 aaatcgatgt cttcgatttt gctgttgagt tttgcggcga tgtagtagcg catccactcg
     3001 gggttcaggc cttgttccaa ataagatttg gcagtgatga aggtgccgcg cgatttggac
     3061 attttttgtc cgtctacggt caaaaagccg tgtgcgtaca cgccggtcgg ggcgcggtgg
     3121 ccggagaaat gcagcatggc aggccagaac agggcgtgga aatagaggat gtctttgccg
     3181 atgaagtggt acatctcggt ttggctgtcg gctttgaagt attcgtcaaa atcgacgccg
     3241 atgcggtcgc acaggttttt aaacgacgcc atgtagccga cgggcgcgtc cagccagacg
     3301 tagaagtatt tgcccggcgc gtcggggatt tcaaaaccga aatacggtgc gtcgcgggaa
     3361 atgtcccagt cggacagggt ggtttcttcg ccttcgccca gccattcttt cattttgttg
     3421 agggcttcgg gttgcagatg gggcttgccg tcgtgcggat tgttgccgga agtccatgct
     3481 ttgaggaagt cggcgcattc gtccagtttg aagaagaagt gttcggattc gcgcaattcg
     3541 ggtttggcac cggaaacggc ggaatacgga ttaatcagtt cggtcgggga ataggtcgtg
     3601 ccgcagactt cgcagttgtc gccgtattgg tcttgggcgt ggcatttcgg gcattcgcct
     3661 ttaacgaaac ggtcgggcag gaacatttgt ttttcagggt cgaaaagctg ctcgatgacg
     3721 cggctttcga ttttgccgtt ggctttcagc gcgcggtaaa tgtcttggga aaactgtttg
     3781 ttttcagggg aatgggtgct gtaataattg tcgtagccga tgttgaaacc ggtaaagtcg
     3841 gcgaggtgct cttcgcgcac tttggcaatc atgtcttcgg gcgcgatgcc ttgtttttgc
     3901 gcggcaagca tcacgggcgt gccgtgggtg tcgtcggcgc agcagtagta gcattcgtgg
     3961 ccgcgcagtt tttggaagcg cacccaaacg tcggtttgga tgtgttcgac catgtggccg
     4021 aggtggatgc tgccgttggc atagggcagg gcggaggtaa ctaagatttt gcgtgtcata
     4081 ttgtgctttg caaacaatgg gtaaaggcgg attataccgc aaatcaaacg gggaaatgcc
     4141 gtctgaagcc tgaaaaatcg ggcttcagac ggcattttgc caatcggcgg gatttattcg
     4201 acggttacgg atttcgccag attgcgcggc ttgtccacat ccgtgccgcg tgcgagggcg
     4261 gtgtggtagg cgaggagctg cacggggata gtatgcacga cgggggacag tttgccgacg
     4321 tggcgcggtg cgcggataac gtgcacacct tcggtggcat taaaattgct gtcgaggtcg
     4381 gcaaagacga aaagttcgcc gccgcgcgcg ccgacttcct gcatattggc tttgactttg
     4441 tccaacaggc tgtcgttggg tgcgatgacg acgacgggca tgttttcgtc caccaaggca
     4501 agcggcccgt gcttcagttc gccggcagga taggcttcgg cgtggatgta ggtgatttcc
     4561 ttcagtttta acgcgccttc gagggcaatc gggtaatgga tgccgcgccc caaaaacagc
     4621 gcgctggttt tcttggcaaa ctgttgcgcc caagcggcaa tttgcggttc gaggtttaaa
     4681 gcgtgctgca cgctgccggg aagctggcgg agttcttcgg tataacgtgc ttcatcttcg
     4741 ccggatacca agccgcgtac cttcgccagc gttaccgcca aaccgaacag tgcgaccagc
     4801 tgcgtggtaa atgctttggt cgaggcgacg ccgatttccg caccggcacg ggtataaagc
     4861 acgaggctgc tttcgcgcgg cagggcggat tccatcacgt tgcaaatgga gaggctgtgg
     4921 cggtgtccca aagatttggc gtatttcaaa gcctccatcg tgtccagcgt ttcgccggat
     4981 tgggaaatgg taatgaccag ttggtccgga tcggcaatca cgctgcggta tcggtattcg
     5041 ctggcgattt cgacgtcggt cggaattttg gcgatggatt cgagccagta tttggcggtc
     5101 agcgcggcgt aataggacgt gccgcaggca aggattttga cgctgcggat gctttcaaac
     5161 acgcttttgg cgtttttgcc gaagttttcg gggatgaagc cgccgtcgag gaaaacctcc
     5221 gccgtgtctg caatcgcgcg gggctgctcg tggatttcct tttgcataaa gtggctgtac
     5281 ggccccagct ccaaagaggc gagcgagagt tcggatacct tgactttgcg ttcggcaggc
     5341 aggccgtttt tatcggtcag ccttttgatg ccgtctgaag ccagcagtgc gatgtcgccg
     5401 tcttcaaggt acgccacgcg gcgcgtaaag gcgatgacgg cggacacgtc cgaagcgata
     5461 aaggtttcat cgtcgcccaa agcgaccaaa agcgggcagc ccatacgcgc cacaactaat
     5521 tcatcaggct tgtcttgggc aataaccgcg atggcgtatg cgccgtggaa acgtttgacc
     5581 gcttcctgca cggcttcaaa cagcctgccg ccgttttgcg cgtattcgtg attgatgctg
     5641 tgggcgatga cttcggtatc cgtttgcgat tcaaaccggt atcccaaacc ttccaagcgt
     5701 ttgcgttcgc tttcaaagtt ttcgatgatg ccgttgtgga cgacggctat catcccaccg
     5761 gaaatatgcg gatgggcatt cggctcggta acgccgccgt gtgtcgccca acgcgtatgc
     5821 ccgatgccga tgccgccgct gatgcctttt tcgcgtgctg cgtcttccat aagctgcacg
     5881 cgtccgacgc ggcgcacgcg tttgattttg ccgtcggtgt tgacggcaat gcctgatgag
     5941 tcataacccc ggtattcgag gcgtttgagg ccgtcggtca gaaaatcgac gacgttgtga
     6001 tgggcgcgga tggcgccgac gataccgcac ataactgttc cttagtatcc ggttgaaaaa
     6061 aaacaggcgc ggacggctcc cgtgccgcac cttcctcttc ggattataaa ccgcctcccg
     6121 cgccgaaaaa cagcaaaatg ccgtctgaag gcttgggctt gctcaaaaaa gggagagatt
     6181 tccccgcctg accgggatgg gcgttcagac ggcattgcct gccgccggtt tatagttttt
     6241 acaaatcaat attgacaagc tgaaaaaaaa aaacaatata ctcgctcggt cttaatgtta
     6301 acggagtatg gaaatgaaac aaatgctttt agccgtcggc gtggtggcgg tgttggcggg
     6361 ctgcggcaag gatgccggcg gttacgaggg ttattggcgc gaaaaatcgg acaaaaaaga
     6421 gggtatgatt gccgtcaaaa aagaaaaagg caattacttc cttaataaaa tcaacgtgtt
     6481 tacaggtaag gaagaatcta tgcttttgtc tgaaaaagac ggcgcgcttt cgataaacac
     6541 ggggataggg gaaatcccga tcaaactttc cgatgacggg aaagagctgt atgtcgaacg
     6601 caggcagtat gtcaaaaccg atgcggcgat gaaggacaaa atcatcgccc atcagaaaaa
     6661 gtgcggacaa acggcacagg catatctcga cgcgcgaaat gcgttgccgt caaaccagac
     6721 gtatcagcag catcaggcgg cgatcgagca gttgaaacgg cggtttgaag ccgagtttga
     6781 cgaattggaa aaagaaatca aatgcaacgg caaaccgaca ttgttgtttt agcgtaggac
     6841 aaccgggagg atgccgccgt ccgaatcgga cgtgcggttt ctgtaccggt acgggcgggc
     6901 aggaatgtcc gctttttttg ttcggacgcg tttgaatacc cgtttgattc cgtccgtttg
     6961 caaagggtat ttttgttcgg gcggaa
//
LOCUS       DAWHXS010000101         6983 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWHXS010000101 DAWHXS010000000
VERSION     DAWHXS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.101"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(94..774)
                     /gene="tsaA"
                     /locus_tag="WIE22_001696"
     CDS             complement(94..774)
                     /gene="tsaA"
                     /locus_tag="WIE22_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ4127751.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEVCIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            888..1523
                     /locus_tag="WIE22_001697"
     CDS             888..1523
                     /locus_tag="WIE22_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ4127752.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKLTGLPDADLTWVAHAMVGTRGYELLDGLPSTKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            1560..2528
                     /gene="waaC"
                     /locus_tag="WIE22_001698"
     CDS             1560..2528
                     /gene="waaC"
                     /locus_tag="WIE22_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ4127753.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVETYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTAPEAGRLKNLEQPYYAALHATSRDSKLWPVENWRALLQKLNEEQQCNIYLPWGS
                     EVEKTRAEQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            2754..3503
                     /locus_tag="WIE22_001699"
     CDS             2754..3503
                     /locus_tag="WIE22_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ4127754.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKTLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            3514..4449
                     /locus_tag="WIE22_001700"
     CDS             3514..4449
                     /locus_tag="WIE22_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ4127755.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            4512..5123
                     /locus_tag="WIE22_001701"
     CDS             4512..5123
                     /locus_tag="WIE22_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ4127756.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(5175..5492)
                     /locus_tag="WIE22_001702"
     CDS             complement(5175..5492)
                     /locus_tag="WIE22_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ4127757.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            5650..6921
                     /gene="purD"
                     /locus_tag="WIE22_001703"
     CDS             5650..6921
                     /gene="purD"
                     /locus_tag="WIE22_001703"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ4127758.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
BASE COUNT         1847 a         1364 c         1882 g         1890 t
ORIGIN      
        1 ttaatccgct ataacttgat attgcggctt atggcgaaca aacaacagcc gtttgttcaa
       61 ctgctatgcg gcaaaaccgg atttttgccc gatttaaagc ggggttgggg aaagatcaat
      121 aacggttgca cggttttcct cgatttgaaa tctgacttcg taatctgcaa tattcatcac
      181 ataaatccgt tcgggaatat tctgataggc ggggcgcgga tcttgggcaa tgctttggct
      241 gataaggttt ttggtgtttg cagataaatt ttccgcgccg atgttttcct gccaaacgac
      301 ttccaactct acgggtttgc cgctgacgaa accggatgcg gcatcgggtt tggattcgac
      361 aaaggggata taaggtttga tgtccacaat cggtgtgccg tccagcaggt ctgcgccgct
      421 gcaatagagg cggacgggtt tgccggtttc gatgcgttcg agtttcagga gcgagagtcc
      481 gagatggttg gggcggtggg ggctgcgcgt ggcgaacacg cccatttttt gtttgccgcc
      541 gagccgtggc gggcgcacca tttgcgccca gccttcatcc aatacgccgt gaaaaataaa
      601 acttatccac acataatcga aatcttccag cccgcgcacg ctgtctgcgg tgaatttggg
      661 attcagctcg atgcatactt ctgcggcaga gaccaaaccg ggctggcggg cgatgccgaa
      721 tttctgtttg tagggcgagc gggcggtgcc gatgggggta atggtataag tcataaggtt
      781 gtggataagt tttttggaaa aaccttatct tatcacaatc ggttttaccg cttttgggca
      841 gtaatttgtt ataatacagg gcattttaat cgcgttgaaa taatgagatg attgtttcca
      901 ttgatgttga tgcacaaaaa acgtttacgc cactgtgtcc tgacgaactg cccgtgaacg
      961 aggggcattt gattgtcgag gagttgaatg cgcaagccgc tttggcggat ttgcgcgtga
     1021 tgacgaaaga tgcgcatcat atggtagcga aatggcttgt ggataaccct gttgatatgt
     1081 tgaagctgac aggtttgcct gatgcggatt tgacttgggt ggctcatgcg atggtcggta
     1141 cgcgcggcta tgaattattg gacggactgc cttctaccaa agaatacgat tattgcgttt
     1201 ggaaaggtgt tgatcctgaa ttgcatcctt acggcgcgtg ttttcacgat attgaggaaa
     1261 aactaagcac agggctgatt gaatggctgc gttgtcaaaa tacggatacg gttattatcg
     1321 gtgggttagc tacggattat tgtgttaaaa caacggtttt gcagttactc aaaggcggtc
     1381 gttggcaggt tgtcgtcaat gaagcggctt gtcggggtat tgcgccggaa accatcgaaa
     1441 cagcatggca ggaaatgcat tctgctgggg caatcatctt aaaaaacgct gatgaaataa
     1501 ataaatatat taataatcaa taagttaaaa ttgtttcacg tgaaaccccc ttcctaaata
     1561 tgaaaatttt gcttgtccgc ttgtctagta tgggcgattt aattcacact ttgcccgcaa
     1621 tcgaagattt ggcgcgacaa tgtcctgatg tagaactgca ttggctatgt gaggctggat
     1681 ttgcagatat tgcgcgcctg catccgtttg taaaaaaaat ccatgtgatg aaatggcggc
     1741 aatggcgcaa acatctcttt cgggctgaaa cttggcggga aatgggtcat ctgaaacaga
     1801 ctttgcggca ggaagtattt gatttcgtat tggacagtca aggtctgatt aaaagcgcgt
     1861 gtttcgccaa aatggcaaaa tccccgattt gtggtttgga taaaaacagt gcgcgcgagg
     1921 gatgggctgc tttggcgtat gtagaaacat acgctgtacc gaagggaaaa aatgccgttt
     1981 ggcgcaaccg tgaactgttt gcccaagtat ttgggtatgt aatgcctgaa acgcaggtat
     2041 ttggcttgac tgctcctgag gcaggtcgtc tgaaaaattt agagcagccg tattatgcgg
     2101 ctttgcacgc gactagccga gacagtaaat tatggcctgt ggaaaactgg cgggcgttac
     2161 tgcaaaagtt gaatgaagaa cagcaatgca atatttacct gccttgggga agtgaagttg
     2221 aaaaaacgcg tgccgaacag attgcagacg gactgccgtt tgctattgtg tgcgacaaaa
     2281 taaatttatt gcaggcagcg tatctgctga aatacgcggt cggaattgtc ggcgtggata
     2341 ccggtttgct gcatttggca aatgccttgg aaaaacctgt ggtcggcatt tataccgata
     2401 ccgatccgat taaaacaggc gttcaggttt cgccaattgc aaaaaatttg ggcaatatcg
     2461 ggcagattcc gaccgcagat ttggtttatc aaacgttgat ggattgtgtt gcagcagaca
     2521 aaggctaaag ggttgtctga aactgatata gcacctgata tttttcagtt tgttaagcat
     2581 aagaatgtat atctaccagc ctgagtaaat gctttaaaaa tggtataatc ggcaagcatt
     2641 atccgaaaga tttttgaatg aattcgtttt taaggaaaac ggatttggtt ttatagggaa
     2701 gaaggtttgg tttcgctgga tggtgaaagc agcgttcttt ttaggagggt tcaatgaaag
     2761 cactggtcgc agtaaagcgc gtagtggact acaacgtcaa agttcgtgta aaagccgatg
     2821 gttcggatgt ggatatcggc aatgtcaaaa tgtcgatgaa tccgtttgac gaaatcgctg
     2881 tggaagaagc tgtccgtttg aaagaagccg gaaaagtaag cgaaatcgta gcggtttctt
     2941 tgggtgagaa aaaatgcgaa gaaaccttgc gtacagcttt ggcgatgggt gccgaccgtg
     3001 ccattcatgt tgaaaccgat actaaactgg agtctctggc cgttgctaag ttgctgaaag
     3061 ccgttgcgga caaagaaaat ccgcaaattt tctttttggg caaacaagcg attgatgatg
     3121 atgccaatca agtggcgcaa atgctggcag ctttgctgaa tgcggcgcaa ggtacgtttg
     3181 cttcaaaagt acacattgaa ggtggtgaag tacagattgt gcgcgaaatt gatggcggcg
     3241 aagaaaccat agcattaaaa ctgcctgctg ttatcagtgc tgatttgcgt ttgaacgagc
     3301 cgcgctttgt caaactcccc aatattatgg cggcaaagaa aaaaactttg gaaaaactga
     3361 ctcctgacga tttggttgcc gacatttcac ctcgtttgaa aacggtgaaa tttgccgaac
     3421 ctaaggcgcg tcaggcaggc gtaaaagtag caagcgttgc cgaattggtt gaaaaattga
     3481 aaaacgaagc caaagtgatt tgaggaggct gaaatgagcg tattgattat tgtcgaacac
     3541 gacaacaaac agttgaatcc taccactttg catgctgtta ccgctgccgc taagctgggt
     3601 aaaatcgatt tattggttgc cggaagcggt gcatctgctg tagtagagtt cgcaaaacaa
     3661 gtggcgggtg tggaaaaagt tttggttgca gatgctgccc attatgccga aggtttggct
     3721 gaagagttgg ctccgctggt tgtcaaattg gcagcggatt actgctatgt tgcagcaacg
     3781 gcaactacat tcggtaaaaa ccttttgcct cgcgtagcag ccttattaga cgtaccgcaa
     3841 atttctgatt tgaccgaaat tgtggataac acgacttttg tgcgccccat ttatgcgggt
     3901 aatgcatttg aaaccgtgca agctgattca gaaaaattgg tgctgacctt ccgtgcgacg
     3961 gtttttgacg cagtggcggc gcaaggtggt aatgctgagg taattaatgt tgaagcaacc
     4021 cctgcccaaa acctgagccg ttttgttaac cgtcagcttt cccattccga tcgtcctgaa
     4081 ctgactcagg caaaagtgat tgtttccggt ggtcgtgcgt tgggtagtgc ggaaaaattc
     4141 aatgaagtgc tgacaccgtt ggcggatgtt ttaggtgcgg caatcggtgc atcccgtgcg
     4201 gcggttgatg ccgaatatgc gccaaacgat gctcaagtcg gacaaaccgg taaagtggtt
     4261 gcgccgcaac tctattttgc aatcggtatt tcaggtgcga ttcaacacgt tgccggtatg
     4321 caagacagta aggtgattgt tgcaatcaat aaagatgctg atgcgccgat tttcaatgta
     4381 gccgattacg gattggttgg cgatttattt gaaatcgttc cacaattaac agaagcattg
     4441 aaaaattaat gtttcacgtg aaacgttcag acgaccttgt tattaatgag gtcgtctgaa
     4501 aacatttcag catgattacg acttataaaa ccattacttc ctcgacgcag gctgagttta
     4561 aagataaagg cagccgtttt attgcatttg cctatccgat tcggacattg gctgatgtga
     4621 aaaaatacct cgatccgtta aaggaagagc atcataaagc acgacactgg tgctatgcct
     4681 atcgtttggg tgtggatggc atgcaatttc gtgccaacga tgatggagag ccatcaggaa
     4741 gtgctggacg accaattttg ggacagattg attcggtggg tattaccgac gttttggtcg
     4801 tggtcgtccg ctatttcggt ggtactttgt taggtgttcc aggtctgata catgcgtaca
     4861 aagaggcgac ggctcaagcg ttggcagttg cagaagtagt tgaaaagaat attgaaaaaa
     4921 ctgtttggct gaaatgtgaa tatccgtttt tgaatgaagc gatacgcatt gctaaacaat
     4981 atcaggcgga tgtattggag cgggatttgc agttggattg ccgattgacg gtcagtttgt
     5041 cgttggtaaa ttatgaggcc tgtgttgcgg cttggaagag tacacggcag attgaagtaa
     5101 atacggaaaa gccttttgaa tgaaaggtcg tctgaaatgc tttgtgatgg tatttcagac
     5161 gacctcttcg ctgactaatc ttcttgtgta tatttgtcta aggcatcgac gctggagcgc
     5221 aaatataacc aagccagttg atggaattcg cccagcgcgt tccatagtgc atcttcactc
     5281 ataatgctgt attcgccttc tgtgcgcaaa tccacctccg ctccatcttc aatagcccga
     5341 tgacaagcct gatattcgtg ggtataaaaa ttgtccatat cgcgaatcaa agcgacggca
     5401 tgtttccaac ggcggatgtc ttcttccaat tgcggtagta aatgacacca ttcgcggtat
     5461 ttgctttgga tttcatcaat acgtttttgc atggttgctt gtcctttttt aatttatgaa
     5521 taagttgaat aggttgcaga gtggaatgaa gcggtctttt gtttgattct actaaaattt
     5581 cttcagatgg gataaaagtc cctttgtatc gagttttcgt taaaagtaca tatttgtcaa
     5641 ggagatggca tgaaactgct ggttatcggt aatggcggtc gcgaacacgc gctggcttgg
     5701 aaattggcgc agtcgcctaa agtggaaaca gtatttgttg cgtctggtaa tgccggtaca
     5761 gcgattgaac ccaagctgca aaacatcgac ttgactgcgc atcaggattt gattgaattc
     5821 tgtcgtaaag aaaatattgt ttttaccgtc gtcggccctg aagcgccttt ggcggcaggt
     5881 gttgtggatg atttccgtgc tgcaggacta aaaatattcg gtccgacaca atatgcggcg
     5941 cagttggaaa gttccaaaga tttcgccaaa gcatttatgg cgaaatacaa tattccgacc
     6001 gcgcaatatc aaacctttga aaacgccgat gccgcacatg attacgtcaa tcagaaaggt
     6061 gcgcctatcg ttatcaaagc cgatggtttg gcggcaggta aaggcgtgat tgtggcgatg
     6121 actttggatg aagcgcatgc tgcgattgat gatatgctgc tggacaacaa aatgggcaat
     6181 gccggcgcgc gtattgtgat tgaagatttc ctgcaaggcg aagaagcgag ttttatcgtc
     6241 atggttgatg gcaataatgt actgcctatg gcaaccagcc aagaccacaa gcgtctttta
     6301 gacggcgaca aaggtctgaa tacgggcggt atgggtgcgt acagccctgc gcctgtggta
     6361 acgcctgttg tgtacgaacg cgcgatgaat gaaattattt tgccgaccgt agcagggatg
     6421 aaagcggagg ggcatgagtt caccggtttc ctgtacgcag gtttgatgat tgatcaaagc
     6481 ggtgcacccc atacgattga gtttaactgc cgtttcggcg atcctgaaac ccagccgatt
     6541 atgagccgac tgaacagcga cttatcggat ttggttgaag cggcaataga cggcaaactc
     6601 gatagcgtaa ccgcggaatg gagcccgcaa actgccgtgg gcgtggtact ggcggcgcaa
     6661 aattaccccg aaacgcccaa aaaaggcgat gttatttccg gtctggatgc tgccaaccaa
     6721 gtaggcaaag ttttccatgc aggtacaacg gcaaacgaga aaggtgacgt attgaccaac
     6781 ggcggacgcg tattgtgtgt tgtgggattg ggcgataatg tggcgcaagc gaaagctaag
     6841 gcatatggcg cgttggaaaa aatcagtttc gacgggatgc aataccgtaa agacattgcc
     6901 gataaagcga ttaaccgtta attagaaaga ggatgatgcc gtctgaagcc aagtatgact
     6961 aatgtgcttc agacggtatt tta
//
LOCUS       DAWHXS010000102         6965 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWHXS010000102 DAWHXS010000000
VERSION     DAWHXS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.102"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1111)
                     /locus_tag="WIE22_001704"
     CDS             complement(<1..1111)
                     /locus_tag="WIE22_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ4127759.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYAGHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAESSGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLQHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALGGDGTLTQALRRALPFALTDAQENVLAEVQTDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGT"
     gene            complement(1197..1640)
                     /gene="secB"
                     /locus_tag="WIE22_001705"
     CDS             complement(1197..1640)
                     /gene="secB"
                     /locus_tag="WIE22_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ4127760.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(1661..1918)
                     /gene="grxC"
                     /locus_tag="WIE22_001706"
     CDS             complement(1661..1918)
                     /gene="grxC"
                     /locus_tag="WIE22_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ4127761.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            2132..3625
                     /gene="rng"
                     /locus_tag="WIE22_001707"
     CDS             2132..3625
                     /gene="rng"
                     /locus_tag="WIE22_001707"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ4127762.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIQPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHRRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(3660..4910)
                     /locus_tag="WIE22_001708"
     CDS             complement(3660..4910)
                     /locus_tag="WIE22_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ4127763.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(4923..5080)
                     /locus_tag="WIE22_001709"
                     /pseudo
     CDS             complement(4923..5080)
                     /locus_tag="WIE22_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(5301..6614)
                     /locus_tag="WIE22_001710"
     CDS             complement(5301..6614)
                     /locus_tag="WIE22_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ4127764.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILMPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1678 a         2065 c         1774 g         1448 t
ORIGIN      
        1 gcgtgccgac ggcgattttg acgctgccgt cggcgagttt ggctttggct tcgtctttgg
       61 cttttttgcg caaactgcca aaaaggcgga caacttcaat gcccaaaggt tcgagccatt
      121 gtttaaattt aataaaatgt tgttcggcaa ggatttcggt cggtgccatt acagccacct
      181 gcgcgccgga ttcgatggcg gtcagcgcgg acaaggcggc gacgatggtt ttgccactgc
      241 cgacatcgcc ttgcagcagg cggtgcatcg ggtgcgtttg cgccatatcg gtttgaactt
      301 ccgccaacac gttttcttgt gcatcggtca gggcaaacgg cagggcgcgg cgcagggctt
      361 gggtcaatgt gccgtcgccg cccaatgccg ccgccgtgcc gctgacgcgc ttctgtctcg
      421 ccagccgcat ggacaactgt tgcgccaaaa gttcgtcgaa tttgagccgt tgccatgcag
      481 gcagcgtgcc gtctgaaagc tgatgaatcg tgaaactcgg cggcggcgaa tgcaaaagac
      541 gcaggctttc ggcgaggtgt tgcagcttca gacggcacag cagggcatcg ggcagcgtgt
      601 cgtgcagcgg cgtaacatcc aacgccgtct gaatgatgcg gcgcaaagtg ggctggttca
      661 aaccgtttac ggtcgggtaa accggcgtga ggctttccgc caaaccactg ctctcggcat
      721 cgcggatttt gggatgaatc atctcgtcgc cgtaaaagcc gtgtttgatt tcgcccacgg
      781 cgcggatgcg tttaccgacc gccgtctgct tctgatggcc ggcgtaaaag tggatgaagc
      841 gcaggaaaag gacgctgccg gagccgtcgg cgatttggac aatcagctgc ttgcgcggtt
      901 tgaacgttac ttcctgatgg ataacctcgc cctcgacctg gcacggcacg ccgatcggcg
      961 cgtccttaat cggcataatg tgcgtctcgt cctcgtaacg cagcggcagg tgcaaaacca
     1021 aatcccacgc ggtatggagg ttgagtttgt cgagcttctt ggcggaaaca tcggtgattt
     1081 tgagctgttt ctgggtttcg ggcgacatca taggcagatt cctttggacg cgcctatttt
     1141 atccgaaaac aaaaatgccg tctgaaacgg attcagacgg catcggcagg caggaatcaa
     1201 gctccggcgg cttcggcttc ctgctgttgt tggtaaatcg cctcaaaatt aatcggcgcg
     1261 agcaggacgg gcgggaagcc ggcgcgcgtt accgtactgg aaacggcttc gcgcgcgtaa
     1321 gggaagagga tgttcggaca cgccacgccc agcagcagct gcacatcttc ttcggggatg
     1381 ttttccaaac ggaaaatgcc gctttgggtt acttcgttca aaaacatcgt gcgctcgtta
     1441 tccaatttgg cggttacggt aacggttaca tccacgttgt agtagccgtc ttccagcttt
     1501 tggctgccgg tggaaacacg catatccact tcgggctcgc cctgttccaa aaagatttgc
     1561 ggcgcgtgcg gcacttccaa agacaagtct ttgacataca gtcgctcgat gctgaatacg
     1621 ggttgcagtt cttcgctcat tttgttttcc tagttggggg ttaagggttc agcagtccgt
     1681 ccagcccgcc ttcctgctgg aggcggtaga ggtcggtaaa tccgccgacg tgcgtttcgc
     1741 cgatgaaaat ctgcggcacg ctgcgctgtc ccgaaagctg ctgcatttcg gcaaaggctt
     1801 cggggcttgc atcgacacgg atttcgtcga tatgtccgac acctgccgcg tgcagcagcc
     1861 ttttcgccat cgcgcagtag ggacaaaacg gacctgtgta catggtaacg gtctgcatat
     1921 tgggtttccg aaagttttgc aatgataatc aatatagggg catttcccct gtttggcaag
     1981 tgcggaacag atgcacgttc aaacggcatg tgcggaatgt gtcaaagttt cttttttaaa
     2041 gtatgatagg cattgtgaaa aatatttttg cacccgcgct gcgcggcgga acggatgcaa
     2101 aaatattttt attacatttt caggaaaaac catgttgtca ggactcccca tccccaaaga
     2161 catcgcgcgc ccgcccgaaa cgatattggt caacatcacg ccgcaggaaa cgcgcgtagc
     2221 ggtgttggag gaaaacaata tctgcgagct gcacatcgag cgcaacagcg aacacagcct
     2281 ggtcggcaat atctatctgg gcgtggtgcg ccgcgtgctg cccgggatgc agagcgcgtt
     2341 tatcgacatc ggcttggaac gcgcggcgtt tttacacatc gtcgatgtcc tcgaacaacg
     2401 ccgcaacccc gaagaaaccc agcgcatcga acatatgctg tttgaagggc agtcggtttt
     2461 ggtgcaggtc atcaaagacc cgatcaacac caaaggcgcg cggctttcca cccaaatctc
     2521 gctggcgggg cgtttcctcg tccatcttcc gcaagaagac cacatcggcg tgtcccaacg
     2581 catcgaagac gatgccgaac gcagcagcct gcgcgaacgc ctcgacaagc tcctgccgga
     2641 aaatgcctgc cggggctaca tcatccgcac caacgccgaa aacgccaccg acgaacagct
     2701 ccagtccgac atcgactacc tgaccaaagt gtgggaacac atccaagaac aggcgaaaat
     2761 tcagccgccg gaaaccctgc tttatcagga tttgcccttg agtttgcgcg tattgcgcga
     2821 tatggtcggc tgcgacacgc aaaaaatcct cgtcgattcc accgtaaacc accgccgcat
     2881 gacgcgtttt gccgaacaat acgtccacgg cgcattgggc aggatagaat tgttcaaagg
     2941 cgaacgcccg ctatttgaaa cccacaacgt cgagcaggaa atcagccgcg ccctgcaacc
     3001 gcgcgtcaac ctcaacttcg gcagctacct gattatcgaa tccaccgaag cgatgaccac
     3061 gatagacgtg aacaccggcg gcttcgtcgg cgcacgcaat ttcgatgaaa cgattttccg
     3121 caccaacctc gaagcctgcc acaccatcgc ccgcgaattg aggctgcgca acctcggcgg
     3181 catcatcatc atcgacttca tcgatatggc gcaggaaagc caccgcgaag ccgtgttgca
     3241 ggagcttgcc aaagccctcg ccttcgaccg tacccgcgtt accctgcacg gttttaccag
     3301 cctgggcttg gtcgagctga cgcgcaaacg ctcgcgcgaa aacttaaacc aagtcctctg
     3361 cgaaccctgc ccttcctgcc aaggcagagg ccgtctgaaa acgccgcaaa ccgtatgcta
     3421 cgaaatccag cgcgaaatcg tccgcgaagc gcgccgttac gatgccgaaa gtttccgcat
     3481 cctcgccgcc cccaacgtca tcgatttgtt tttggacgaa gaatcgcaat ccttggcaat
     3541 gctgatagat ttcatcggca aaccgatttc tctggcagtc gaaaccgctt acacgcagga
     3601 acaatacgac atcgttttga tgtaaaaaat gccgtctgaa gctttcagac ggcatctgtc
     3661 tatttcaggg tttccttgtc caacaacgcc cgtatcaaca gaccgtgtcc aaaacgtcgg
     3721 ctgtcggtca gttccaaata tccgccgtat tttttggcaa gcgtgtcggc gatggacagc
     3781 cccagccccg tcccctgctg ctccgttccc aaaatacggt aaaacggatc gaggacgcgg
     3841 gcgcgttcgg attcgggaat gccgttcccg ttatcttcca cccacacggc gagatatttc
     3901 ccttcgtccg tgaaacccaa atcgatcctg ccttcgggcg gcgtataacg tattgcgtta
     3961 tcaacaaacg ttttaaccaa ggtatagatt tcagtttcat cggcaaatat ttcgacatca
     4021 cctccgaccg ccacaccaat gtcctgacgt ttttccaagg caagcggcat cagttcctgc
     4081 aacacttggc ggaaacggct ttgcagaccg aatgtcgttt tcgccaaagg ggtttcgtcc
     4141 gactgcgaac gcgccagtgc caaaagctgc tcgagcaggt gtttgttgcg ccgtatgctt
     4201 tgctgcaaaa cggcagactg ccgcgccgca tcgggtggca gagacatatt gttgagccgt
     4261 tccgcctgaa gggaaagggc tgtcatcggc gtacgcaatt cgtgtgccgc gtcggcgaca
     4321 aaccgctgac ggtggcggat gtcttcatcc gcacgtttca aaagcaagtt gatggcggtt
     4381 acgaaccctc tgatttcact gggaatattg tccacactca aggcagacag gtcattgatt
     4441 cggcgttgtt cgagactttg cgacaatttg cggacggggc gcatggcttt gtgcgtaatc
     4501 cacacggtca gcagtatcat cagcggcagt gccgccagca ggggcaacac gctttgccgt
     4561 gccgcatccg ccgccaaatc ttcccggtat tcgctttcct gcatcacggc aatccgcccc
     4621 cgctccgtcg tgcggatata gacgcggtaa taatcgtcgt ccccgtccgc ctgaagcgtg
     4681 tgcagaccgt ccgccagatg cgcaggcagg ctgacaacag ggtcttcctg ctgcggcatc
     4741 tgtaccaaaa tacgcgtatc gccgtcgccc tcgggcaaag tttcgggttt ggaatcgggg
     4801 gcgacgtaca atgccgcctg acggagcagg tcgtcctgca acgcttccgt ttcgtggaag
     4861 gtttcgtagt aggaaaacat acctgcaagc attgccagcg gaacaaacat ccaaaccgtc
     4921 cgccccggca aacccaaatc cagcagcatc aagtcataag gctgggcagc ggcagccgcg
     4981 cgccgttttt gacccaatcc accgcatagc cgccgtcttt caaacttgcc gacaccgcct
     5041 ccgcaatcat cgcatcgtct tccaccagca aaacacgcat caactttccc tttcagacaa
     5101 accgtgccta ttctaacacc ccaaaattag ccgcaattta gcggccttta cgcttgccgg
     5161 tatttttcaa aactgcagca caaaaaaacc gcgccggcaa ctgccttcag acggcattgg
     5221 ggcgcgattg caacacacgg gcaggcaggc agcctgcgac agaccacagg aacgattcgg
     5281 gcttcagacg gcttcgccgt ttacggcaga ggcacgattc ctgccgctat cgaactggcc
     5341 aatatcgcca gcgacaaacc ccacgcccaa aaaaacgaat aacggatgtg tttgcccatc
     5401 gacaatttcg ccaaacccaa gcccatccac aaagccggcg aaagcggcgt aacaaaagtg
     5461 ccgacgatac tgccgatcaa catcgcataa cctgctgctt cgggcgccac gcccgcctgc
     5521 gaggtaatct gctccacaat cggaaacagt ccgaaataat aagcgtccgt actcaaaacc
     5581 aactcaagcg gaatgcccaa cacaccgatg gcaatatgca gataaggcag cagcgcgtcc
     5641 ggcaggatat gcacaatgtc tttggaaatc gcgtccaaca tccccgcacc cttcaaaatc
     5701 cccaaaaacg tacctgccgc caaaataatg gacgccatca tcaccgcgcc gccggcgtgg
     5761 gcataaatcc gctccatctg ttcctgcggg ctgcggtaat tcaaaagcaa cgccgccgtt
     5821 gcagccagca taaatacata acccggcggg aagatgcccg aaaaaagcag gctcatcgcc
     5881 gccaaaaaca gcaggacatt ccaccaaaac agtttcggac gcgccaattt ttgttcttct
     5941 tccgacaaag gcaccggctt tatcaaatcc gccacggcgg gcaacgcgcc caactcccgg
     6001 acaatccgcc ttttttcacg cacacccaaa agcagggaca gcgcaaggat aaacactaca
     6061 ccgataattt gcaccgtcaa caaaggttta tacaattcgc ccacatctgc gcccaacacg
     6121 cttgcaaccc gcccagtcgg cccgccccac ggcagaaggt taatcaatcc cgcactggaa
     6181 gtcagcagca aaaacagcag gtaaggattc atatgcagac gcttgtaaag cggcaaaagg
     6241 gcggggacga ccaataaaaa cgtcgtcgca cccgccccgt ccaactgcgc caccaccgac
     6301 accaagaccg tccccacact cactgccacg atattacccc gagtcagctt aatcaaaccg
     6361 cctatcatcg gacggaacag ccccacatcg ttcatgattc caaaaaacaa aatggaaaac
     6421 ataaacataa tcacaatctg catcaccgat ttggtgccgc ccgaataaaa ttcttttaat
     6481 tgggatacat caaaccccgc cagcaacgcc ccaaacagcg gcatcaagat taatgcgatg
     6541 atgggcgaca ctttttccgt cagcagcaac catacgatga ccccgataat cagcagtccg
     6601 ataaacgtca gcatcatttc tcctttattt tattttaaac agaaaaccga ccgtgcaggc
     6661 aaaaccgccc acagacgcgg gataagcctt gcattctact tttttatttt gaaacaagtc
     6721 aatcggtcat ttcctcccat ttacgcctgc cgccattcct gtatccgtcc gtcatttcac
     6781 ggcggcaacc gatacggaac aaccggcaaa ccggtatcgg gacggcgcgg gggcattcat
     6841 cccggtacgc cgattcaaac gaaaccgccc ctatcattgc ggagcgcggg gcgtgccgta
     6901 cacgtgggat tttatagtgg attaacaaaa atcaggacaa ggcggcaagc cgcagacagt
     6961 acaaa
//
LOCUS       DAWHXS010000103         6865 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWHXS010000103 DAWHXS010000000
VERSION     DAWHXS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.103"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..878)
                     /gene="trkA"
                     /locus_tag="WIE22_001711"
     CDS             complement(<1..878)
                     /gene="trkA"
                     /locus_tag="WIE22_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ4127765.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSAT"
     gene            complement(976..2499)
                     /locus_tag="WIE22_001712"
     CDS             complement(976..2499)
                     /locus_tag="WIE22_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ4127766.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVES"
     gene            complement(2806..3612)
                     /locus_tag="WIE22_001713"
     CDS             complement(2806..3612)
                     /locus_tag="WIE22_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ4127767.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALKKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(3664..3915)
                     /locus_tag="WIE22_001714"
     CDS             complement(3664..3915)
                     /locus_tag="WIE22_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127768.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4015..4776
                     /locus_tag="WIE22_001715"
     CDS             4015..4776
                     /locus_tag="WIE22_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127769.1"
                     /translation="MDVNKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRDHVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMALFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            5075..6244
                     /gene="metZ"
                     /locus_tag="WIE22_001716"
     CDS             5075..6244
                     /gene="metZ"
                     /locus_tag="WIE22_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ4127770.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            6339..6545
                     /locus_tag="WIE22_001717"
     CDS             6339..6545
                     /locus_tag="WIE22_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ4127771.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(6644..6756)
                     /locus_tag="WIE22_001718"
                     /pseudo
     CDS             complement(6644..6756)
                     /locus_tag="WIE22_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1502 a         1688 c         1968 g         1707 t
ORIGIN      
        1 gttgccgaac cttgcaggac gagggtgttg tcgaggtttt cggctatcca ttcggcacgg
       61 cgcggccggc attcgatgat tttgacgttg tatgcgtgtt cgagctgctt ggcgagacgg
      121 tagccgatgt tgccgccgcc ggcaatcatg atgcggcggg tgctggtttc tttggggcgc
      181 aattcgggta tgaccgcgcc gatgttttcg gcggcggcgg caaataggat ttcgtcgcct
      241 tcgatgatga cggtttgcgg cgcggggacg atgaggcggt tgttgcggta aacggcgcag
      301 atttggcagt cggccccgtc gggcaaatct tgggcgatgt cggcaatgct gcgtccgaca
      361 agcagtccgc cgcgccgcgc ctgtatgatg accatccgca cgcggtcgtc tgcaaaacgt
      421 aaaacctgca atgcgcccgg gcagtctatc aggccggcaa gctgttcggt aacgagctgt
      481 tcggggctga tggtttcggt tatgccgaat atggaaaggc tgccgttttc gttgttttcg
      541 agcttggggc tgaggtattc gaggtattcg ctggaacgga cgcgcgcgat gcggccgggg
      601 atgttgaaca ggtcggcggc aactttgcag gcgacgatgt tggtttcgtc gctgcgggag
      661 agcgcgagca gcaagtcggc atcttccgcg ccggcgcgtt ctaatgtgaa gggggatgcg
      721 ccgttgccga aaacggtttg gacatcgagg cggctgcctg tttcctgcaa tgctttttcg
      781 tcgatgtcga taacggttac gtcgttgttg ggtatggcgg caaggttttg tgcgacggta
      841 gaacctacct gtccgttgcc taaaatgagg attttcacgg tgtgggtcgc cgagtgatga
      901 aatggtggat tgtaccgcaa ccgggggcgg aaatgccgtc tgaaggcaga ttttgtgttc
      961 agacggcatg gcgcctcaag attcgacggg gatgatgccg attttcgcct gccattgggg
     1021 cggggcggtg gcgtggatgg attcgccttt gctgtctacg gcgacggtta cgggcatgtc
     1081 tttgacttca aattcgtaaa tggcttccat gcccaattcg gggaacgcca agactttgga
     1141 agatttgatg gcttttgcca cgagatacgc cgcgccgccg actgccatga ggtacacggc
     1201 tttgttgtcg gcgatggctt cgcaggtggc cacgccgcgc tcggatttgc cgatcatgcc
     1261 caagaggtcg gtttgttcga gcatttggcg ggtgaatttg tccatgcggg tggctgtggt
     1321 cggacctgcc ggaccgacga cttcatcgcc gaccggatcg acgggaccaa cgtagtaaat
     1381 caggcggttg gtgaaatcga cgggcaattc ttcgcctttg ttgagcatat cgacgaggcg
     1441 tttgtgtgcg gcatcgcggc cggtgaggat tttgccgttc aacagcaata cgtcgccggt
     1501 tttccagctt gccacttctt ctttggtcag cttgtcgaca tcgacgcgtt tgccgttgtc
     1561 ggggctgtaa gtcaaatcgg gccagtcttc gacgcgcggc ggcgtgagtt cgacagggcc
     1621 tgagccgtcc aattcaaatt cgacgtggcg ggtggcggcg cagttcggaa tcatggcaat
     1681 cggtttggag gcggcgtggg tcgggtaatc gaggattttc acgtccaaca cggtggtcag
     1741 accgcccaaa ccttgtgcgc cgatgcccag cgcgttgact ttttcaaaga gttcgaggcg
     1801 cagggcttcg gtggtggaca attccgcgcc ggacgcggct ttttcctgca attcttggat
     1861 gtcgatgtgg ctcatcaggg actcttttgc catcaacacg gctttttcag gcgtgccgcc
     1921 gatgccgatg cccaagatgc cgggaggaca ccagcccgcg cccatcgtcg ggatggtttt
     1981 caatacccaa tcgacgatgt tgtcggaagg attgagcatg gcgagtttgg atttgttttc
     2041 agaaccgccg ccttttgccg cgcaggttac ttcgactttg tcgcccggca cgatgctcat
     2101 atggatgacg gcgggcgtgt tgtctttggt gttttgacgt ttgccggccg ggtcggcgag
     2161 aacggaagcg cgcagcgtat tgccttccca agtgtaggcg cggcgtacgc cttcgttaac
     2221 catttcttcc acgctcatgt ccgcatccca ctgcacatcc ataccgactt tcaaaaacac
     2281 ggttgcgata cctgtgtctt ggcagatggg gcggttgttt tcggcacaca tgcggctgtt
     2341 gaccaaaatc tgcgtcatcg cgtctttggc ggcggggttt tcttctttct gccacgcttt
     2401 atagagcgcg tcgatgtagt ctttgggatg gtagtagctg atgaattgga aggcatcgca
     2461 aatgctttgg ataaagtctt cctgtttgat gacggtcatg attgtgttcc ttttcaaggt
     2521 tgtggcggta agtgtctgct gttgttttaa gcaacctttt tgaaggctgt tttttatcag
     2581 gttgaaatag tgccagcgtt tgtacggtaa agacaataca taattctaat ctcttgatta
     2641 tcaatttgtt ttaataaaat actgttttac tcggattttg atatttgtaa tctttgcctg
     2701 ttgctttgcg gtagggagaa atcagcaggc agttcattgt aggccgattg atgaacaatg
     2761 ccgtctgaaa gggcttcaga cggcattgtg ttcggatggg cgggcttatt ttgcggcctg
     2821 tccgtcttct tttgcaaaat atttggcgta gattttgtcg tattcgccgc tttcgcgtac
     2881 ttttttcaac gcatcgttca gcattttgac ggttgcttcg tcgcctttgc gtaccgcgat
     2941 gccgtagtgt tcggtggtga agtcgggcag ggtaacgaag tccattcctt tggtcggatt
     3001 gtttttcaca taattggcga tgaccgcgct gtcgctgact acggaatcta agccgccgtt
     3061 ttccagttct ttgataatca ggggaacgtt ttcaaagcgc gcgattttcg ggttgtcgtt
     3121 gcccaagagt ttggatacgg agaaatcgcc cgtgtagccg gttaccacgc cgactttgtt
     3181 catgtttttc aaatcttcgg aagaagatat ttttttgcct ttcggaacga ggacgacttg
     3241 ggtgatttca aaatacgggt cgctgaagtc catagactgt ttgcggtcgt cggtaatggt
     3301 tacgcccgac atcacaacgt ccgcatcgcc gttgttcaag gcggggaaaa ggctgtccca
     3361 cggctggtgt ttgaattcga ttttaaaatt gcccgccttc gccatcgcgt tcatcaaatc
     3421 cacatcgaaa ccttcgacat tgcctttcga gtctaaagat tcaaaggggg caaactcggc
     3481 gttggaagcc acgcggtaca ctttgtcggg gtttgcggcg ggcgcggcgg catctttacc
     3541 ctgaccgccg caggcagaca gcgcgagcgc ggaacaggca agggcggcgg caatccattt
     3601 tttcatattc atcaaaattc ctttcggtaa tcaaaaagcc taaaaaatca gtttaacata
     3661 aaattaacgt tctttttcac ggaaaaacgc ataagtaacg tcgtggaaca aatcggtatg
     3721 ggggctgtcc tcatagctca attctgccaa ggcgtcctca aaagcaagct gaatcgactc
     3781 gaccgcgatt tcggcggttt cggcgggaag ggcgcggatt aaggctttga atgcggtgga
     3841 caggacgtgg ttctgcatac gcagcacgtc gttcgcctct tccagatatt ccaaacgctg
     3901 ttcgatgctg cccatgatgt atcctcgcga atctgttaaa atgcgccatt atatcaaacc
     3961 gcgctttggg cgcgggtttg tctgcctgca aagattaaga accggaagcc gaaaatggat
     4021 gtgaataaag agccgaattg ggaaaaacgt ttggaaaacc tgcgtcagac gcgccgtatg
     4081 aaggcagtgc cgacttacgc gccgctgtcc cgcccctctg aaaccggaac ggacgcgcct
     4141 gtgccgaaac tggacgatga gagccgagac catgtgttga aggcgtatct gaaaaaatgg
     4201 caggaggaac acaatgccgc cgttgccgat tgcggcgagg aagtcgaaac cgacccgatg
     4261 attgtccttc aggaaaactg gctcaacgcg caggcttccc tgcaaatgtc ggtttcggaa
     4321 aaacacatcg aatcgcggcg gagaatcctg ttcgatgcca aaaaagacag tgccgaattt
     4381 gaaaccggac agattgaagg cggggaaaac gccgatgcgg cagaatcggg tacggcggca
     4441 gacggggttg cggaaaacgg cgaaggcggt gcggaagagg cgttgatgcc cgtccaaatc
     4501 aatatcctga acccgaaagc cgtcaaccgg cgcgaagtgt tctgcctgtc cgaacaggag
     4561 ctgaccgaac ggctgatcaa acgcctgcgt ccgcatctga ccgataccgt caacggcatc
     4621 attcgaacgg cggtgcaaaa gcagatggca ctctttactt atcagctcca gcaaatgctg
     4681 cacgagcagg cgggcgcggt ggtcgaagac gtgttggaac acgatgtccg caaaatcctc
     4741 aacgacatca agtacgagtt aaaatacaaa cgctgaccgt catgccgtct gaaagccgtg
     4801 ttcagacggc atttgcattc tcatccgact tgaaagcctc acggaacggg cgcaaaatat
     4861 taatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgaagaca gtacaaatag
     4921 tacggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
     4981 cgaggcaacg ctgtactggt ttttgttaat ccactataaa ttaaacaacg ggcgcacgct
     5041 ccgttgccga acggataaaa ccgaggaatc accgatgagc aaaaaactcc atccgcaaac
     5101 gctcgccata cgcggcggca aagaacaaac cggataccgc gaacacaatc aggcattgtt
     5161 cctgaccagc agtttcatgt gggacaacgc ccaacacgct gccgatttgt tttcaaaaaa
     5221 aatcaaaggc ttcacttata cccgtaccgc caacccgacc acagccgcct ttgaaaaacg
     5281 catcgccgct ttggaaggtg cggaacgcgc ggtcgccact tcgaccggca tgtccgcgat
     5341 tcaggcggcg tttttcacct ttttgcaggc gggcgaccat gtgatttcca gccgcagcct
     5401 gttcggcacg accgtcggct ttatcaataa catcgttacc aaattcggca tcgaagtgag
     5461 tcacgtgtcg ccgactgata taaacgaatg gaaagccgcc gtcaaagcca acaccaaact
     5521 gctgtttttg gaaacgccgt ccaatccctt gggagaagtg gccgacttgg aagccttggc
     5581 ggaattggcg cacggcatcg gcgcgctctt ggtggtggac aacagcctgc tgtcgcctgt
     5641 cggctctcag cctttgaaac acggcgcgga tatttccgtt tcctccgcga ccaaagccat
     5701 agacggacac gggcgcgtga tgggcggcgt gttggcgggt tcggaagaac tgatggcgca
     5761 ggtcgccgtt tactgcaact cttgcgggct ggcaatgtcg ccgtttaacg catggcagtt
     5821 gttgagcggc gtggaaaccc tgtctctgcg tatggaaaaa cagttcgaca acgccttgaa
     5881 aatcgcgcaa tggctgcaag cgcagccgca ggttcaagcc gtgtattaca ccggattacc
     5941 cgaccatcct caggcggagc ttatccgcaa acagcaaaac ggcggcggca tcgtcatcgg
     6001 ctttgaagtc gccgaccaag cagccgcgtg gaaagtcgtg gacggcgtcg aacttttctc
     6061 ccgtaccgcc aacctcggcg acgtgcgctc gaccattacc catccgtgga caaccacgca
     6121 cggcaggatg cagcccgaag aaaaactcgc cgccaacatc cgccccggat tggtgcgcct
     6181 gtcggtcggt ttggaatatg ttggcgattt gattgacgat ttgaaacagg cactcgcgcg
     6241 ctgattgcgt gttcagacgg catcggcgga aaaacaccgc aaatgccgtc tgaaaccctt
     6301 atcttatatg acatacacgt caacaatagg aaaaaaatat ggatgaagcg gtttttgccg
     6361 attgggcgtt gaaaatctgc ctgaccggct tgattatttt tttgggtttt atcgtttgga
     6421 atttgggcaa ggagtctaaa gcgggcaaat tcggcatcgc cattttgttt ttggttttgg
     6481 gcttgggcgt gtttggtttt gtgttcaaag agttattgat taagtttttg gtattgccga
     6541 aataggtttg cggtctgtca aaatgccgtc tgaaatcata aaaagtaatc tgcaccctaa
     6601 aagtttgatt taaccgccaa ctgattaagg tgcggatttt tatgacaaaa tatagtggat
     6661 taacaaaaac cagtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
     6721 cgtcgccttg tcttgattta aatttaatcc actataaaaa tcatctttcc gcagtcattc
     6781 ccacggatgc gaaagcctgt ctttttgaat cccggataat tttacatcgt cattcccgcg
     6841 caggtgggaa tccagatcat tgggt
//
LOCUS       DAWHXS010000104         6789 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWHXS010000104 DAWHXS010000000
VERSION     DAWHXS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.104"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(499..1791)
                     /locus_tag="WIE22_001719"
     CDS             complement(499..1791)
                     /locus_tag="WIE22_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ4127772.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1868..2836
                     /locus_tag="WIE22_001720"
     CDS             1868..2836
                     /locus_tag="WIE22_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127773.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(3037..4503)
                     /locus_tag="WIE22_001721"
     CDS             complement(3037..4503)
                     /locus_tag="WIE22_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4127774.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4745..6789)
                     /locus_tag="WIE22_001722"
                     /pseudo
     CDS             complement(4745..6789)
                     /locus_tag="WIE22_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1426 a         2035 c         1739 g         1589 t
ORIGIN      
        1 ttgcaaacac tgccagaacg gataaatccg gattcccgcc cacttcgtca ttcccgacgc
       61 gcacgccgct ttcccgccca cttcgtcatt cccgcgaaag cgggaatccg gtgcgttggg
      121 tttcagtcat ttccgataaa ctcctgccgc attgtatttc tagattcccg ctttcgcggg
      181 aatgacggcg gatgggtttc cgtaccgtgg tgtccggatt cccgacggta cgggaatgac
      241 ggcagtgctt ccttaactta gttttatttt aatcgactgt atggtggatt aacaaaaatc
      301 aggacaaagc gacgaagccg cagacagtac aaatagtacg aaaccgattc acttggtgct
      361 tcagcatctt agagaatcgt tctctttgag ctaaggcgag gcaacgacgt actggttttt
      421 gttaatccac tataaatgcc gtctgaaaac ctgtttgcag gttttcagac ggcattgttt
      481 tcgtcgccgg ttcaaacttc aatccgattg cgacacggta accggtatgc cgcgcaagcg
      541 cgcaagcagc gcgtccgcct tctgtttctg cgcggcaaac gccgccttgt ccgcctgcgt
      601 caattcgggt gtcggcaatg cgactgaaac aggattaacg ggctgcccgt tgatgcgcgc
      661 ctcgtaatgc aggtgcggcc cggtcgaacg cccggtcgaa ccgacaaaac cgatgacctc
      721 gccgccgcgc acattgcctt gtgcctgcga aaacgcgctc aagtgcgcgt acagcgtttc
      781 cacgccgttg gcgtggcgta tcatcaccgc gttgccgtat ccgcccttcc ggcctttaaa
      841 ggtaatcacg ccgtcggcgg aagccctgac cggcgttccc tgcggtgcgg cataatcgat
      901 gcccgtgtgc agccgccatg tgtgcaggat ggggtgcata cggtagccga acggcgaaga
      961 aatgcgcgta tagaccagcg gctcgatgtt gaagccgcct ttttcctgca acaccctgcc
     1021 gtcttcatca taataattgc cgccccctcc gccttccttg tccgaacggt aatagaatgc
     1081 ctgatgcctt gtgccgccct taacgacttc agccgccaaa atatcgcccg ccgccacctg
     1141 ctgcccgtgg aaatacaggc tgtcgtacat caggcgcacg gcatcgcctt ccttcaaacc
     1201 gtcaaggctg aagcggccgg cgaaaatccc gcttaaggat tcgcggattt cgacgggcac
     1261 ttccgcccgc gccagcgaac cgcgcgccga cgttttgacc acgaccgaac gcagcgtcgg
     1321 caaaaccttc atatccgcct cagaagccga ccgccgccat atgccgcctt tcttttccaa
     1381 agcgaccaga ttgcgctcgc cgtcttcgtc ggtaaaaaac tgtacttcgc gcgcgccgcc
     1441 gtcgccgccg accaaaacat gaaccgactg gtcggcacgc aaatgccgca aatcggcttc
     1501 gccgccatat ttttccgtga ttcgggcaat ctcgtcccgc gccatacccg aacgcgccag
     1561 cacgtccgcc agcgagtcgc ccggctgcac cgcctcctgc acccaatatg ccgtctgaac
     1621 gccgctgccg ccccaagaca gcggcggcag attttgttcc acgcgctgcg gtctgacgcg
     1681 ttccgtcctc tctgtcaaag caatgtatgc cgccgacacc aaaataatcg aaacggcaag
     1741 cgcgcgcagc gcgtatttcc gatgttttgc cgaaagtggg aagacagcca tattgttcct
     1801 ttttgacggc gtttgcgccg aatcctctat aatatccgcc cgatccgcat ttgtcagaca
     1861 tcaaacgatg aaactcaccc tcgccctccc ctcgctcaat ttggatgaag acgaaatccg
     1921 aatacctcta tgcctgcccg ccttcaacaa aatcctgcaa tacggctcgc tgcaccgcca
     1981 aagctgcacg gcttcggcat tttacgcgcg ttatttgtgg tgcggcagac tggcggaacg
     2041 cgcggcgcaa agcctgaata tgccgtctga aaccgtcgcg ctcgccacgc ccgtctggca
     2101 aaagatgggg atgcatcagg caaatgtgct gacggcggaa tatttggatg tcggtacgga
     2161 tgaagcggaa cggctctgcc gcgatctgtc ggcgttttac ggcgatattc cgtggcgttt
     2221 cgttcccgtc ctgcccgaat tatggctggt ttcccttccc cgcgcgtacc gctggggcgc
     2281 gaagcccgtt ttggacttgg gcggattgtt gggcgcggac gatcagccgg acggagaaga
     2341 tgcgttggaa tggctgcgcg tccaaaccga aatccaaatg tggctggcgg cgcaccccgt
     2401 caaccataac cgcaagaaac gcgggctgcc cgagttgaac ggcttatggc tttggggcgg
     2461 cacaaacggc ggcacgcaag gcggcacgct tttcgccgat acggtttgga gccgtttcca
     2521 ccccgaccgc cgcgcattgc ccgacaactt ccgcgcctat gaagaaacgg cggcacacct
     2581 gcccgacacg caccatatcc tgtttatgga cgatttgcgc ctgaccgccc tgacagggga
     2641 cagggagcgg tatgcggcaa tattgcagca gtgggaagaa cgctggttcg caccgcttta
     2701 cgaagccgtc cgcatcggca aaattaaacg gctggacatc gccacggacg gacaacacgg
     2761 cggtacgctg accttcaaac ccgccgacag gtggaaattt tggcgcggcg cgaaaacctt
     2821 tgacggaatc tggtgaaccc gcccgccgca accgcgacaa tgccgtctga agcccgaatc
     2881 gggtttcaga cggcatttta cctcgcatcg ccgttatgcc ggaaaaaccg gaatgcgttt
     2941 ccgcacacgc ggcggcacgc aaagcaccgc ccgaaagccg ccgatgcccg ccgtgatacc
     3001 gccgccggca gggtttcctc cgaaaaggaa cagcaatcag aacgttttat taaactcgac
     3061 aaacgcccga tttttctcgt attcgttgaa cacatcgtta ctccgcgttt cgcggtgcga
     3121 caacgtcagg cgcggcgtga tgcctttgaa atgcaatgcc cggtgccaaa ggctcaagga
     3181 tgtgttcaat tctttatccc tgcgcctttc ccctttaaaa ccgctgaaaa agccgggttt
     3241 ttcataatgc cgtttcgccg cgccgaggcg caacagcgaa gacaggccgc tgccgcccca
     3301 ttcctgcccc caggcaaagc gcaggccgta acggttgaaa ttgtcgcccc ggtcggcggg
     3361 gttgcgctcg cggtaaaaat ccaaaccgcc catccaatat tggcgcgcat tccggtaaaa
     3421 caccagcgaa ttggaaattt gcaaatgggt attgtcggaa cgcgcccggc gcgtattctt
     3481 caaacgcccc cactccgccg aagacaacgt ttgccatttc ggggtttgcc aacggttgaa
     3541 ataaaggcgt gcgccgttgg tgtaagaata agcgtcgttg ccgtaggtgc ggcgttcgtg
     3601 gaacactgcc agcccggcat ctttgcgccg gtcggcaaaa ccgatgccgc cggaaacgcc
     3661 tgccgtcata tcgttgaatt tcttattccc cggataaacc ctgccggaca cgtcgccgcc
     3721 cgccgtcgtg taccagccgt ttttcagcga ccattttttc tccgcgccga gccggtaatt
     3781 gaccgccgtg ccgtccacct gtttcgggaa agtccatttg ccgtactgct gccgtttcgg
     3841 ggcttggttg atattgtgtt cgcgggtgac gctgaagccg ccatttacct tccacgcatc
     3901 gcgttcgcgc aatgccttgc ggtacagctc gacctgctcc atcagctgcg gcggcaggtt
     3961 ttccgccttc aggcggtcga actggtctgc cgccgcctcg ttctgcctgt tttcaaacaa
     4021 tgccgccgcc aaacgcatac ggacggcggg cgcgtcgggt tgggcggcaa tcaattcccg
     4081 gtaatgggaa atcgcctcct tcaccctacc gtctgcctgc gccaaaatcc cttgtgcata
     4141 aagtgccaac atcttatcct gctgcgcctg ttgtaggtaa atcggcaaaa taacgcggat
     4201 accggcaata ttgtttgaga ccactgcgga atacatcgcg cgggacaaca attcgggatt
     4261 tttcagcagg gtttctccgt caatctgcaa aacttttcct ttttcccgca cctgccccgg
     4321 taccttctcc ctgtcgattg gtttgacctc cgcttcatga agcctgaact cgggacggct
     4381 tctcaaatcc ggttcgcgcg gtgtttcttc ggcatatgcc gcgcttgcca ataaaggcag
     4441 cagcagcatc catctgtttc gtgcaggcat aaatgtcttc ccacaaaaat aaaaaataac
     4501 cattatcaat cggcggattg tatcagtttt ttatgccgct gttccaaacc ctgccgcccg
     4561 atgccgcgac aatgccgtct gaagccagaa tcgggtttca aacggcggca aaaaaccggc
     4621 ggttttccgc cggtttcatc atgatgcccc gcccttcggg gcggcagggt tcggattggt
     4681 ttgcggggcg gcgcgcgggg ccgtgccggt gtgccgcctg tgcgtcaaaa catttcccga
     4741 cgcatcaaaa cagccatttg agcgacaatg cggcttcttt gtcgccgtcc gtccttttgc
     4801 cgtatccgat gcgtgcggac atatggcctt tccaaccggc ttcaatgccg aaccgccctt
     4861 cgagcgccgt cctgcctgcc agcgtctgtt tttcgccgtc catttccacg ccgaaagatt
     4921 ttgacctgtg caaaacatta aaagcggcga aaggctgaag attgacaccg ttacgcaaag
     4981 caaaacgggt ttttgcccga ataccggcgc ggctttgcca ctgaccgctg ccgagcagtc
     5041 cgaccgccgt cccctcgctg tcggtaaagc cgccgtttac gcccaagtag gtaaactgag
     5101 cctgcggttg caggtaaaac cgcacattat tgccttttcc gacaacgcct tccgccacaa
     5161 gcgcgttgta gccgccttcg acagaagccg tccaaccttt ggttttgtag cgttccacac
     5221 ggttttcatc attgatgcgg tgtttgaaac gttggtattg caaccagccg tccaaatacg
     5281 cacccgtttg tttatcgcgc aactgatgcc acgcagcata aacacccccg ccataaccat
     5341 gcaaataact gcctgccgca ccgcctttgc cgttgactga tgcgtgctgg ccggccctgc
     5401 cgcccatcac gccgattgcc agccggctgc cttcattttg ccgtacaaac acctcgccgc
     5461 cgatttgcac gcctttgcgc cgcccgtccg cagccgcgcc gccccgtata ttttgatgcg
     5521 accggccgcc gatgaagcgc agccacattt tttgacggga cggatcggcg gcatacacgt
     5581 cgtctcccct gtcggcggca cgcagcccaa acaaagtatt tgccgcataa gcctgttggg
     5641 catataaaac cgcttccgcc tcggggatag ccaaacgctg cacgcccaag caaaatccgc
     5701 cttcgcattg cttgagcaat aattcgtaca gtccctgccg gtaattttgt ttaaacgcaa
     5761 acgaattagg gtctgccttt tccgccgcaa tcagtttgcg cccgtcgagc gttttcagat
     5821 tttccggccg gcgttccaaa accacgccgg attgtccgct gactttgtct tcaaaggtca
     5881 gggcgggttc gtccgactcc cgcacgtcgt agccgaacag gaaatccgcg ccgccctgat
     5941 acgctttatg gacggtcagt ttttcatctt tcgtgttcag gcggatccgg gcattatcgg
     6001 aagtttccag catatggatg tcggaatggc ggcgcggttc ccaaagggaa tctttcagcg
     6061 tcatcttggt cagttccaac acctgtccgg cgacgtggga tttgttttcg attttgacgt
     6121 gtaatttttt attgaagatg ttgttgagac tagtctgtga tgtattacta tgaggagtga
     6181 gtacggaatc ttctaccaat ttattaatta aatcggggtt tttctgtttg agtatactaa
     6241 attttggtcc aagctgttct atatacgcct cctcagtccg ttttttattt tcttcccaag
     6301 cttcgggtct tgttttgtat aaatcctgta attgcttctt aaccgcatcc tttaaattat
     6361 ccccgtaagt atctgtaata tatttcttaa caacgggcat attatcgacg gcgagtatgc
     6421 cgctattgta gtttttacct tggatattga tttcgtcata cccatattcg gatgaatttt
     6481 gatcggtttt accgctggaa aaataatggg aagtgccggg tttcggcttg tctgaaaatg
     6541 aaaaatcggc atcgtcgccg attttggtag aacgagtatt tgaaccaagc tgagctctga
     6601 acataccgaa gcaatcaaaa gatatgctgc acgggccgcg ccaaacgtgg gaatttctgt
     6661 cttccgccgc ttctaaaata aatttgtttt taatctcggc ttctttgacg ggatcgtacg
     6721 catccgaacc tcaaacatat ggcgtgcaaa acagcgataa aacagataat gttaatagat
     6781 taacccgac
//
LOCUS       DAWHXS010000105         6485 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWHXS010000105 DAWHXS010000000
VERSION     DAWHXS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.105"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            182..2401
                     /locus_tag="WIE22_001723"
     CDS             182..2401
                     /locus_tag="WIE22_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4127775.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSIETTPVNQPAVGAAMRLLRRNTAFHREDGTAIPNSKQAEEELSFKEE
                     DILFLYGSKKDQRKQLEDKIHQRNPNVKIRTSENENGKDSYKFVDAGYVYAKGEDEIK
                     RISNYRQISNRLGYDGFVYYSGEHPSQSLPSAGMVEYSGNWQYMTDAKRRRTGQAVAS
                     EDLGYTTYYGNEIGATSYAARDADDRERHPAKYTVDFYKKTLTGQLIKNQYVQNKSNL
                     NEPKNPLTIYNITAKLDGNRFTGSASVNPDLATNHANKEHLFFHTNADQRLEGGFFGD
                     KGEELAGRFISNDNSVFGVFAGKQKTEAANAADTKPALPSGKHTKILDSLKISVDEAA
                     DKKARPFEVSSMPDFGHPDKLLVEGREISLLKDTQTIVLADGRKKTIRACCDFLNYVK
                     IGRMQTERPAVKPKAQDEEGDEEDAGVDNGEDEEEIAEESEDEVSEDEGEEDEEIVEE
                     ETEEEAEPEEAEETEEESPAESGSGSNAILPAPEAPGRDIDLFLKGIRTAETDIPKTG
                     EARYTGTWEARIDKPIQWDNQADEKAAKAVFTVDFGKKSISGTLTEQNGVKPAFHIEN
                     GKIEGNGFYATAHTRDTGINLSGTGSTNPTTFQANGLRVEGGFYGPQAEELGGIIFNN
                     DGKSLGITEGTENKVEAEAEAEAEAEVEAGVEQLKPEIKPQFGVVFGAKKDNKEVEK"
     gene            2398..5229
                     /gene="lbpA"
                     /locus_tag="WIE22_001724"
                     /gene_synonym="tbpA"
     CDS             2398..5229
                     /gene="lbpA"
                     /locus_tag="WIE22_001724"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ4127776.1"
                     /translation="MNKKHGFQLTLTALAVATAFPSYAVNPETATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTHDIQSNKTGQFFLVEGENTLK
                     PVAKLAGYGIYLNNQLNRWVKERIEKKQPLSAEEEAMVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKVVLSFDKQGVATDNNTLKLNCAVYPSVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDDKNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTQLPDSTGRLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(5938..6485)
                     /locus_tag="WIE22_001725"
                     /pseudo
     CDS             complement(5938..6485)
                     /locus_tag="WIE22_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1796 a         1536 c         1690 g         1463 t
ORIGIN      
        1 gatgtaggtt cgtgggaatg acgggatgta ggttcgtggg aatgacggaa tgtatctgta
       61 ctgtctgcgg ctcgtcgcct tgtccggatt tttgttaatt cactataaaa acggcttgat
      121 attatctgta catattaata taatgataat tattattaat caaataggag gaaaagtagg
      181 gatgtgtaaa ccgaattatg gcggcattgt cttgttgccc ttacttttag catcttgtat
      241 cggcggcaat ttcggtgtgc agcctgttgt cgaatcaacg ccgaccgcgt accccgtcac
      301 tttcaagtct aaggacgttc ccactccccc ccctgccggg ccttctatag aaaccacgcc
      361 ggtcaaccag cccgccgttg gtgcggcaat gcggctgttg agacggaata ctgcttttca
      421 tcgtgaagat ggcacggcaa ttccaaatag caaacaagca gaagaagagc tgtcgtttaa
      481 agaggaagat atcctgtttt tatacggttc aaaaaaagat caacgtaagc agcttgaaga
      541 taaaattcat caacgcaatc ctaatgtaaa aattaggaca tcagaaaatg aaaatggaaa
      601 agatagttat aaatttgtag atgcaggtta tgtatatgca aagggagaag atgaaattaa
      661 gcggatttca aattacaggc agatttctaa tcgtttaggc tatgacggtt ttgtatatta
      721 ttccggagaa catccttccc aatctttacc gagcgcggga atggtggaat attccggtaa
      781 ctggcaatat atgaccgatg ccaaacgtcg tcggacaggt caggcggttg ccagtgagga
      841 tttgggttat accacatatt atggtaatga gattggtgca acttcttatg cggctaggga
      901 tgccgatgac cgagaaaggc atcctgccaa atatacggtt gatttttata agaaaacttt
      961 gacgggtcaa ttaattaaaa atcagtatgt gcaaaataaa agtaatctaa atgagcccaa
     1021 aaacccactg accatttaca acattactgc aaaattggac ggcaaccgct ttaccggcag
     1081 tgccagtgtc aatcctgatt tagcgacaaa ccatgccaat aaggagcatt tgtttttcca
     1141 taccaatgct gatcagcggc ttgagggcgg ttttttcggc gataaggggg aagagcttgc
     1201 cggacggttt atcagcaacg acaacagcgt attcggcgta ttcgcaggca aacaaaaaac
     1261 agaggcagca aacgcagcag atacaaaacc tgccctgccg tctggaaaac acaccaaaat
     1321 cttggattct ctaaaaattt ccgttgacga ggcggctgat aaaaaagccc gtccgtttga
     1381 ggtttcctct atgcccgatt ttggtcatcc cgacaaactt cttgtcgaag ggcgtgaaat
     1441 ctccttgctc aaagatactc aaaccatcgt tcttgccgac ggcaggaaaa agacaatccg
     1501 agcctgctgc gattttctga actatgtgaa aatcggacgg atgcaaaccg aacgccccgc
     1561 cgtcaaaccg aaggcgcagg atgaagaggg ggacgaagag gatgcaggcg ttgataacgg
     1621 cgaagacgaa gaagaaatcg ccgaagaaag cgaagacgaa gtttccgaag atgaaggcga
     1681 agaagacgaa gaaatcgtcg aagaagaaac cgaagaagaa gctgaacccg aagaagccga
     1741 agaaaccgaa gaagaatcgc cggcagaaag cggcagcggt tcaaacgcca tcctgcctgc
     1801 cccggaagcc cctggcaggg acatcgacct tttcctgaaa ggtatccgca cggcggaaac
     1861 ggatattccg aaaactggag aagcacgcta taccggcact tgggaagcgc gtatcgacaa
     1921 acccattcaa tgggacaatc aggcggatga aaaagcggca aaagcagtat ttaccgttga
     1981 tttcggcaag aaatcgattt ctggaacgct gacggagcaa aacggtgtaa aacctgcttt
     2041 ccatattgaa aacggcaaga ttgagggcaa cggtttctac gcgacagcac acactcggga
     2101 taccggcatc aatctttcgg gaactggttc gaccaacccc acaaccttcc aagctaatgg
     2161 tcttcgtgta gaaggaggat tttacggccc gcaggcggag gaattgggcg gtattatttt
     2221 caataatgat gggaaatctc ttggtataac tgaaggtact gaaaataaag ttgaagctga
     2281 agctgaagct gaagctgaag ctgaagttga agctggtgtt gaacagttaa aacctgaaat
     2341 taaaccccaa ttcggcgtgg tattcggtgc gaagaaagat aataaagagg tggaaaaatg
     2401 aataagaaac acggttttca gcttaccctg accgccttgg ctgttgcaac cgccttcccg
     2461 tcatatgcgg taaaccctga aacggcgaca ccagatgcgg cgcagaccca atccctgaaa
     2521 gaggttaccg tccgtgccgc caaagtggga cggcgatcga aagaggcgac cggtttgggc
     2581 aaaatcgtca aaacgtcgga aacgttgaac aaagaacagg tactcggtat ccgagatctg
     2641 acgcgctacg atccgggcgt ggcggttgtc gaacaaggca acggcgcgag cggcggctac
     2701 tcgatacgcg gcgtggataa aaaccgtgtg gcggtttcgg ttgacggcgt tgcccaaata
     2761 caggcgttta ccgtgcaggg atcgttgagt ggatacggcg gacgcggcgg cagcggtgca
     2821 atcaacgaaa tcgaatatga aaacatcagc acggtggaaa tcgacaaagg cgccggttcg
     2881 tccgatcacg gcagtggcgc actcggcggc gcggtcgctt tccgcaccaa agaagcggca
     2941 gacctgattt cagacggcaa aagctggggg atacaggcaa aaaccgccta cggcagtaaa
     3001 aaccgccaat ttatgaagtc gctcggcgcg gggttcagca aagacggttg ggaagggctg
     3061 ctaatccgta ccgaacgtca agggcgggaa acgcgcccgc acggcgatat tgcagacggg
     3121 gtggcatacg gcataaaccg tttggatgcg ttccgccaga cacacgatat tcaaagcaat
     3181 aaaacagggc aatttttctt agtagagggc gagaatacac tcaagcccgt ggcaaaattg
     3241 gcgggctacg ggatatattt gaacaaccag ctcaaccgct gggtaaaaga acgtattgaa
     3301 aaaaaacagc ctttaagtgc tgaagaagaa gcgatggtgc gggaggcgca ggcgcgccac
     3361 gaaaacctgt ccgcccaagc ttacacgggc ggcggcagga tattgcccga tccgatggat
     3421 taccgcagcg gctcttggct tgccaagctg ggctaccgct tcggcggcag gcattatgtc
     3481 ggcggcgtgt ttgaggatac caaacagcgt tacgacatcc gcgatatgac ggaaaaacag
     3541 tattacggta cggacgaggc ggacaagttt agagacaaga gcggggtgta cgacggcgac
     3601 gatttccgcg acggcttgta ttttgtgccg aatatagaag agtggaaggg cgataaaaag
     3661 ttggtcaggg gcataggttt gaaatattcc cgcaccaaat ttattgacga acatcaccgc
     3721 cgccgccgta tgggtttgct gtatcgttat gaaaacgaag cgtattctga caattgggcg
     3781 gacaaggtgg tgttgtcgtt tgacaaacag ggcgtggcaa ccgacaacaa cacgctgaag
     3841 ctgaattgcg ccgtgtatcc ttccgtggac aaatcctgcc gcgcgtcggc ggacaaaccg
     3901 tattcctacg acagcagcga ccgtttccac taccgcgaac agcacaatgt tttgaatgcc
     3961 tcgtttgaga aatcgctgaa aaacaaatgg acgaaacacc atctgacttt gggcttcggt
     4021 tacgatgctt ccaaagcgat ctcccgcccc gaacagcttt cccacaatgc ggcaaggatt
     4081 tcggaatcca cgggattcga tgataaaaat caagataagt atcttttggg taagcccgaa
     4141 gtcgtcgaag ggtcggtctg cggctacatc gaaactttgc gttcccgcaa atgcgtgcca
     4201 agaaaaatca acggcagcaa tatccacatt tctttgaacg accgtttttc aatcggcaaa
     4261 tattttgatt tcagcttggg cggcaggtac gaccggaaaa acttcaccac gtcggaagaa
     4321 ctcgtccgca gcgggcggta tgttgaccgt tcgtggaaca gcggcatcgt gttcaaaccg
     4381 aaccggcatt tttctttgtc ttaccgcgcc tccagcggct tcagaacgcc ctcattccaa
     4441 gagcttttcg ggatagacat ttatcacgat tatccgaaag gctggcagcg tcccgccctg
     4501 aaatcggaaa aggcagccaa ccgcgaaatc ggtttgcagt ggaagggcga tttcggcttt
     4561 ttggaaatca gcagcttccg caaccgctat accgatatga ttgccgttgc cgatcacaaa
     4621 acccaattgc cggattcaac aggacgattg acagagattg atatacgcga ttattacaat
     4681 gcccaaaata tgtcgctcca aggcgttaat atattgggaa aaatcgactg gaacggcgtg
     4741 tacggcaaac tgcccgaagg tttgtacacc acattggcgt acaaccgtat caaaccgaaa
     4801 tcggtatcca accggccggg actgtccctc cgcagctatg ctttggatgc ggtacagccg
     4861 tcgcgttatg ttttggggtt cggatacgac cagcccgagg ggaaatgggg cgcaaacatt
     4921 atgctgacct attccaaagg gaaaaaccct gacgagcttg cttatctggc aggcgatcaa
     4981 aaacgatatt cgacaaaaag agcgtcgtct tcttggtcga cggcagacgt ttccgcctat
     5041 ctgaatctga aaaaacggct gaccttgagg gcggctatct acaatatcgg caactaccgc
     5101 tacgttactt gggaatcctt gcgccagacc gcggaaagca cggcaaaccg gcacggcggc
     5161 gacagcaact atggaaggta tgccgcaccg ggcaggaact tcagcctcgc gctcgaaatg
     5221 aagttttaaa ggaaataccg tctgaaagct tgatctgcac cccaaaagtc ggactaaacc
     5281 gccaactgat taaggtgcag gtttttttga ttcaatataa acaagatttc cgtcgtcatt
     5341 cccgcgcagg cgggaatcta gacattcaat gctaaggcaa tttatcggga atgactgaaa
     5401 ctcgaaaaac tggattccca cttttgtggg aatggcggca gagcggattc tgttgctcct
     5461 gataaatgcc gcaatctcaa atcccgtcat tcccgcgcag gcgggaatct agacattcaa
     5521 tgctaaggca atttatcgga aatgactgaa actcaaaaaa ctggattccc actttcgtgg
     5581 gaatgacggc agagcggctt ctgttgctcc cgataaatgc cgcaatttca aatcccgtca
     5641 ttcccgcgca ggcgggaatc tagacattca atgctaaggc aatttatcgg aaatgactga
     5701 aactcaaaaa actggattcc cactttcgtg ggaatgacga atttcaggtt gctgtttttg
     5761 gttttctgtt tttgtgaaaa taacaggatg agacctttgc aaaattcctt tccctcccga
     5821 cagccgaaac ccaaacacag gttttcgtct attttcgccc caaataccgc ctaattctac
     5881 ccaaatatcc ccttaatcct gcccggatac ccgataatca ggcatccggg ctgcctttta
     5941 ggcggcagcg ggcgcactta acctgttggc cgctttcaac aggttcaaac acatcgcctt
     6001 caggtggctt tgcgcactca ctttaatcag cccgaaatag gctgcccgcg catagcggaa
     6061 tttacggtgc agcgtaccga agctttgttc gaccacataa cgggtcttcg acaaataccg
     6121 gttgcgtttg gtttgcgctt ccgacagcgg acggttgcgg caggctttgc gcataatgcc
     6181 gtccagcaac tgatgttctt ccagatgttg ccggttttcc gcaccgtcgt agcctttatc
     6241 ggcatagacg gtcgtacctt cgggcagtcc ttccaacaac ggcgacaggt gtttgcactc
     6301 atgggcattg gcgggggtaa tgtgcagttt ctcgatatag ccttccgcat cggtacgggt
     6361 atgttgtttg taaccgagtt tgtagagatc caacgggcat ctttgtcctt actcggtgtg
     6421 gtttggccgc tgatttgtcc ttcttcgtca acttctatgg cctgacgctg tttgctgccg
     6481 gcggt
//
LOCUS       DAWHXS010000106         6445 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWHXS010000106 DAWHXS010000000
VERSION     DAWHXS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.106"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            362..808
                     /gene="yciA"
                     /locus_tag="WIE22_001726"
     CDS             362..808
                     /gene="yciA"
                     /locus_tag="WIE22_001726"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ4127777.1"
                     /translation="MQHEEGNRQRPQGELLLHTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
     gene            complement(859..1680)
                     /locus_tag="WIE22_001727"
     CDS             complement(859..1680)
                     /locus_tag="WIE22_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ4127778.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQVLADES
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1950..2408
                     /locus_tag="WIE22_001728"
     CDS             1950..2408
                     /locus_tag="WIE22_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ4127779.1"
                     /translation="MNIQTRLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFDSDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2551..3687)
                     /gene="lst"
                     /locus_tag="WIE22_001729"
     CDS             complement(2551..3687)
                     /gene="lst"
                     /locus_tag="WIE22_001729"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ4127780.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKDFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKNQSYGKSK"
     gene            3788..3892
                     /locus_tag="WIE22_001730"
                     /pseudo
     CDS             3788..3892
                     /locus_tag="WIE22_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3915..4055
                     /locus_tag="WIE22_001731"
     CDS             3915..4055
                     /locus_tag="WIE22_001731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HEZ4127781.1"
                     /translation="MPPHIRHSTSDTAAKSGKTISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            4122..6347
                     /locus_tag="WIE22_001732"
     CDS             4122..6347
                     /locus_tag="WIE22_001732"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ4127782.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEAVGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1700 a         1895 c         1466 g         1384 t
ORIGIN      
        1 ctttcgtggg aatgacgtgg tgcaggtttc cgtatggatg gattcgtcat tcccgcgcag
       61 gcgggaatcc agacccctga cgcggcggga atctatcgga aatgactgaa accccgagat
      121 tctagattcc cgcctgcgcg ggaatgacgg ttcagttgct tagggtagga tttggcggga
      181 ttggtgggct gaagcccacc ctacggcccc gccctacgca tccgccctac aatgcgcctt
      241 gcgaatctgc cctacgcccg cccttgtatg tctcaaatgg gttaaactac gcacttcccg
      301 acttcccgtt tcaggcaggg gtgcaggtcc gatttatatt tcaataaaac aaggaaactt
      361 tatgcagcac gaagaaggca accgccaacg ccctcaaggc gaactgctcc tgcataccgt
      421 cgctatgccg cgcgatacca atcccaacca agacattttc ggcggctgga ttatgtcgca
      481 gatggatttg ggcggcggca tattggcggc ggaaatcgcg cggggacgca tcgttaccgt
      541 cgccgttcag gaaatgaact tcatccgccc ggtcaaagtc ggtaatgtcg tctgctgcta
      601 cgggcattgc gtccgcgtgg gcaatacttc cctccagctt aaagtggaag tctgggtgaa
      661 aactttgatg aacgattgcg ttaccgaaga ccgccatctc gtaaccgagg cggtgttcac
      721 ttatgtcgcc atcgatgcgg aaggcaatcc gcgcccgatt ccgaaagaag gcaaccccaa
      781 attggcgggc ttattgccta ctccgtaaaa atacccgtaa aaatgccgtc tgaaactgtc
      841 ttcagacggc attccgcatc atttgaacca agacctgatg cgggaaaaca gcgacatact
      901 ctgtttttca aagtcttccc gcacttcggg cacggctgtt tttatgacct tcattcctgc
      961 aaaagtctgc gccacgggca taatttcaat cgtgttcaca ttcatatgcg ccggccggcg
     1021 gtacagccac aatgcggttt ccgcaatatc ttcggggcgg ataaattcca caccctcata
     1081 cacacccgcc gccctctcgt catcgccttt gaagcgcacg ttggaaaact ccgtattgcc
     1141 gcacaaaccc ggctcgatat tggtaacgcg gatgttctta tccgccagct ccgcgcgcaa
     1201 attcaggctg aactgccgca caaacgcctt ggttgccccg taaacgttgc tgccggcgta
     1261 agcataattg cccgcaatcg aacccaaatt catcacataa ccgccgccgc gttccaccat
     1321 ttgcggcaaa attttgcgcg tcagaaacgt caaacccaaa acattggttt gaatcattgt
     1381 ttcccaatct tcaaaatccg ccttgtccgc cgtgtccaaa cccaaagcca gcccggcatt
     1441 gttgatgagg cagtcgatgt cggaaaattc atcggggatg ccgtttaagg cgttttccac
     1501 cgactcgcgg cgcgacacgt ccatttccaa agggtaaaac aaagcacccg attcatccgc
     1561 caaaacctga agcctgtccg cacggcgcgc cgcaccgata acgcggtatc ccgccccgac
     1621 aaacgcgcgg cacatcgctt cgccgaatcc tgccgaagca ccggtaatta aaacagccat
     1681 tgtttttcct ttctttctcg tttcccaaac ctgtttcagg tatcataacg cctttcagac
     1741 ggcatcgcgc cggctgaaaa tgacaacaac cataccacca cgacaggaca cattcaatat
     1801 gtttcaacac acaggacgac acataaagcg tcgccctatg tgccgtcctg attcggaagg
     1861 gattgcaccc ttcccaaata aaatctgatt ctaccgcccc gaaggacgga tgtccaagtg
     1921 ggcggggttt caaccgaaaa ggaaacacga tgaacattca aacccgcctt gccgcaaccg
     1981 ccctcgccct attcctttcc gcctgcggaa acggcggcgc gcccgcacag cccaaaggcg
     2041 aaatttccga aaaccgcacc gccgcgttca aatctatgat gcccgacttc tcgcgtatgg
     2101 gcaaaatggt caaaggcgaa gagccttacg atgtcgaaaa attcaaacag gctgccgcgg
     2161 cgtttgccga aagcagcaag aaaccgttta cgctttttga ttccgatccg caaggcaacg
     2221 gccgcgccct gccagccgtt tggtcggatg gtgcaaaatt tgaagccgaa aaaacaaaat
     2281 tcgccgccgc cgtcgaaaaa ctcaacgccg ccgcccaaac cggcaaactg gacgaaatca
     2341 aagccgccta cggcgaaacc ggcgcaagct gcaaatcctg ccacgacagc ttccgcgcgc
     2401 ccgaataaca tccaaaacaa aacaatgccg tctgaagcct tcagacggca ttttctttgc
     2461 catttcacca agcgcaaagc aatcagaaat tatcgtcaat tcaaaaaata cgccatatcc
     2521 tgatttttgc cttacttact ccgccatttt ctattttgat ttaccatacg attgattttt
     2581 atcgtcaaat gtcaaaatcg ggatgccgga ttgggtaaac agggcataca ccggcttgag
     2641 ccaataatct tccggaaggg aagccggttt caatgcgtaa acgtgcacat tgggaaaatc
     2701 cttcatcgtc aacgccgcgc cgctgaaaaa ggtataaatt tcatacctcg tatgcgggtt
     2761 tttcttaatt tcgcgcaaaa tatagtcttc gatgacatag ggcgaattta atgtggttac
     2821 gccggaaagc ccgtaggttt ggcgggggtg cggcgcgaca tattgtatgt tgaaatcttt
     2881 tgccgccttt tccgaaattt ccttcatctc tttgtcgggt gaacccaaaa gtatccgcac
     2941 cgtgccgccc gtttcgtccc ccgtcttcag ttcggacgca tcgaacagcg gcaggtaagt
     3001 catcttgcgg cggccgtcgt ccataatgtt tttcaaaccc ttgaatatcg tgtaatgctc
     3061 gtcggaagca ttgcgggttt tggcgatgct ccaatctccg atcatcatcc gggcaaaatt
     3121 ccgcttgatc gtcccgttta cagaaaactc atcgcccaaa tagctgctgc tttgaattaa
     3181 attgcctgtc ccgtcgtcaa aggttttgat ttccgcatcc gggtaagtgc tcaaaaaggc
     3241 ggcaatgctg actttttcca aacttgccaa ataaatccgt ttgaccttcg gcagcagcat
     3301 cgactttacc ttcagctccg ccatcgtcgg aatgaaatta aacgatttgt tcaaaccgta
     3361 gggcaggtgg aaaaagtacg cccgctccgc tttatccttt atctgcttga aataataatc
     3421 gtatttttca ttcctgtttt cagacatcag caccacataa aaccgctccc ccggatgctg
     3481 cgccataatc ctttccgcca ccttcatctg caatatggta tagcagaaaa tcagattgac
     3541 cggttccccc tcttcattga agagtttgtc cttcagcagg gaaaccgcat tcctttcccc
     3601 ctgatttacc cggtcaaatg tataaaatat cccgaaacaa aaaacaatca aacacaacac
     3661 ggtcaaacaa gcctttttca agcccatatc cctaaaactc cattccgaca aattgaacat
     3721 acgccccgag tgtcccggga cggaaaaatg cggaatttta gcagatattc cgacagttac
     3781 cgaatattat agtggtttaa atttaaatca ggacaaggcg acgaagccgc agacagtaca
     3841 agcagtacga caaggcgagg caacgctgta ctggtttaaa tttaaaccac tataaaatca
     3901 atttcaccga aaatatgccg ccgcacatca ggcacagcac ctccgacacc gccgccaaaa
     3961 gcgggaaaac catttccccc ttcctttcac gccgccgcgt tcaaacacgc caagatcgaa
     4021 caaagcgcga acaaagccgc caaaatatgc cataatagcc acgcacattc tccgcgcaat
     4081 gtgttccaaa agaaaaaacc aacctacaag agaataatcc tatgactcaa aaatccacca
     4141 ttgtttatac ccataccgac gaagcccccg cgctggcgac ccaatcgctg ctgccgattg
     4201 tgcaggcttt tgcccgccac gccgatattg atgtcaaaac tgccgacatt tccctgtccg
     4261 gccgtatttt ggcggcattc cccgaatatc tgaccgaggc gcagcgcgta cccgacgcgc
     4321 ttgccgaatt gggcgaactg gtaaaacaac ccgatgcaaa cgtaatcaaa ctgccgaaca
     4381 tcagcgcatc cgtacctcaa ctgaccgccg cgattaaaga attgcagtct aaaggctttg
     4441 ccgttcctga ctatcctgcc gaccctcaaa ccgatgaaga aaaagccgta cgcgaacgct
     4501 acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg tgaaggcaac tccgaccgtc
     4561 gcgcccccaa agcggtgaaa aactttgcca aaaaaaatcc gcacagcatg ggcgcatgga
     4621 caaaagactc caaaacccac gttgccacca tgcaaagcgg cgactttttc cataacgaac
     4681 aatccgttac cgtacctgaa gcgacttccg tatccatcgt gttcaccgac aaacaaggca
     4741 ataaaaaaga gctgcgcgcg cctgttgccc tgaaagccgg cgaaatcatc gacgcaaccg
     4801 tgatgagcaa aaaagccctg ctcgccttcc ttgccgaaca agtgaaagac gcgaaggcaa
     4861 aaggcgtatt gttctcactg catatgaaag ccacgatgat gaaagtgtcc gacccgatta
     4921 tcttcggaca cgccgtcaaa gtgttcttcg cgcctgtatt tgaaaaattc ggcggcaaac
     4981 tggctgccgc aggtgtcaac gtcaacaacg gcttcggcaa cctgctggcc aatctggaca
     5041 aactggatgc ggacacccgc gccgccgtcg aagctgaaat cgccgccgtt tacgccgcca
     5101 accctgattt ggcgatggtt gattccgaca aaggcatcac caacctgcac gttcccagcg
     5161 atgtcatcgt cgacgcttct atgcctgcga tgattcgtaa ttccggccgt atgtgggaca
     5221 aaaacggcaa agcacaagac accaaagccg tgattcccga cagcagctat gccggcgttt
     5281 accaagcaac catcgacttc tgccgcgaac acggcgcatt tgacccgaca accatgggta
     5341 ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga atacggctcg cacaacaaaa
     5401 cctttgaaat cgaagccgtc ggtcaggttc aagtcattga tgcggaagga aaagtcctga
     5461 tgcagcacga cgttgaagca ggcgacatct ggcgtatgtg ccaaaccaaa gacgctccgg
     5521 tcaaagactg ggtgcaactc gccgtcaacc gcgcccgcct gagcaacacg cctgccgtgt
     5581 tctggctcga tgaaaaccgt ccgcacgaca agagcctgct cgccaaggtt aaagcctacc
     5641 ttgccgaact ggataccaat ggcctcgaca tccgcgtcct cgctcctgaa gaagccgcca
     5701 agttcagctt gggtcgtctg aaaaacggcg aagacaccat ctccgtaacc ggtaacgtcc
     5761 tgcgcgacta cctgaccgac ttgttcccga ttttggaact cggcacaagc gcgaaaatgc
     5821 tgtctatcgt tccattgatg aacggcggcg gtatgtttga aaccggcgcg ggcggttctg
     5881 caccgaaaca tgttcaacaa ttcctcgaag aaaaccacct gcgttgggac tctttgggcg
     5941 aatttctcgc actcgccgta tcgtttgaac atctggcgca aaaaaccggc aacaccaaag
     6001 cccaagtcct cgccgacact ttggatgcag ccaccgaaaa actgctgttg aacgacaaat
     6061 cgcctaaacg taaagccggc gaactcgaca accgcggcag ccatttctac ctcaccctct
     6121 actgggcgca agaattggcg gcgcaagaca aagatgccga actgaaagcc gcatttacgc
     6181 cattggcagc cgctttgacc gccgacgaag cgaaaatcgt tgccgagctt tccgccgtac
     6241 aaggcaaagc ggccgacatc ggcggctact acgccgccaa tcctgaaaaa gccgcacaag
     6301 tgatgcgtcc gagcgtaacc tttaatcagg cactaaacgc cttataagca caaaggtaaa
     6361 aatgccgtct gaacattcgt cgttcagacg gcattagtta tccctatctg cctgattatg
     6421 attaaacgcc gcacccattg gatat
//
LOCUS       DAWHXS010000107         6398 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWHXS010000107 DAWHXS010000000
VERSION     DAWHXS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.107"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            22..405
                     /locus_tag="WIE22_001733"
     CDS             22..405
                     /locus_tag="WIE22_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ4127783.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            698..1171
                     /locus_tag="WIE22_001734"
     CDS             698..1171
                     /locus_tag="WIE22_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ4127784.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            1297..2400
                     /gene="mnmA"
                     /locus_tag="WIE22_001735"
     CDS             1297..2400
                     /gene="mnmA"
                     /locus_tag="WIE22_001735"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ4127785.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQSTDKPVIITR"
     gene            2471..4141
                     /locus_tag="WIE22_001736"
     CDS             2471..4141
                     /locus_tag="WIE22_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ4127786.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4253..5886
                     /locus_tag="WIE22_001737"
                     /pseudo
     CDS             4253..5886
                     /locus_tag="WIE22_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
     gene            5901..6103
                     /locus_tag="WIE22_001738"
                     /pseudo
     CDS             5901..6103
                     /locus_tag="WIE22_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6135..>6398
                     /locus_tag="WIE22_001739"
     CDS             6135..>6398
                     /locus_tag="WIE22_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4127787.1"
                     /translation="MIAKHIDRFPLLKLDRVIDWQPIEQYLNRQKTRYLRDHRGRPAY
                     PLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIP"
BASE COUNT         1669 a         1743 c         1603 g         1383 t
ORIGIN      
        1 gaacattttg aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa
       61 ggcggcatca ggcgcgtcat taacgcattc ggctattcga tagacggcat cgccgccgcc
      121 taccgttacg aagcggcatt ccgtcaggtt ttgtggctga acgtactgct ggtgtgcgcg
      181 gcattttttt gggtttccga aaccgccgtc cgcctgccgt tgattatcgc gtcttttgtg
      241 tcggtcattg tcgaactgtt caacaccgcc gtcgaagctg ccgtcgatca tacttcgacc
      301 gaaaaacacg agctggccaa acgcgccaaa gacgcaggtt ctgccgcaca attgttcgcg
      361 atgctgatgt tggcggcggt ttggctgtct gccctgttcg ggtaaaacgc ttgcagcagg
      421 atttgtaatc ctttaggatt agtatgtggt atttattatt cacttaatct acatcaaatt
      481 tccaggcagt atttgtatgt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
      541 cagacagtac aaatagtacg gaaccgatcc acttggtgct tcagcacctt agagaatcgt
      601 tctctttgag ctaaggcgag gcaacgctgt actggttttt gttaatccgc tataagatta
      661 agcacattcc ccgtctgata ttataaggag cagaaagatg aaaaaattat tggcggccgt
      721 gatgatggca ggtttggcag gcgcggtttc cgccgccgga gtccacgttg aggacggctg
      781 ggcgcgcacc accgtcgaag gtatgaaaat aggcggcgcg ttcatgaaaa tccacaacga
      841 cgaagccaaa caagactttt tgctcggcgg aagcagcccc gttgccgacc gcgtcgaagt
      901 gcatacccac atcaacgaca acggcgtgat gcggatgcgc gaagtcgaag gcggcgtgcc
      961 tttggaggcg aaatccgtta ccgaactcaa gcccggcagc tatcacgtga tgtttatggg
     1021 tttgaaaaaa caattaaaag agggcgacaa gattcccgtt accctgaaat ttaaaaacgc
     1081 caaagcgcaa accgtccaac tggaagtcaa aatcgcgccg atgccggcaa tgaaccacgg
     1141 tcatcaccac ggcgaagcgc atcagcacta atctgctgaa aatgtttgaa atgccgtctg
     1201 aaaaagcccg ggctttcaga cggcattttt atgaccgcct ttaaaatgtg ttaaaatctg
     1261 cctttaaaaa ccgccgtttc caaccatatt ctacgcatga atacgacagc aaacaccacc
     1321 aatatcatcg tcgggctctc cggcggtgtc gattcttccg taaccgccgc cctgctcaag
     1381 cagcagggtt atcaagtgcg cggtgtgttc atgcagaact gggaagacga cgacaacgac
     1441 gaatattgca gcatcaaaca ggattcgttc gatgccatcg ccgttgccga tattatcggc
     1501 atcgatatcg acatcgttaa tttcgccgcg caatataaag acaaagtctt tgcttatttt
     1561 cttcaggaat acagtgcggg gcgcacgccg aatccggatg tgttgtgcaa cgccgaaatc
     1621 aaattcaaat gctttttgga ctacgccgta gggcagggcg cggataccat cgccaccggc
     1681 cactatgcgc gcaaagaagt tcgcaacggc gtgcattacc tgctcaaagg tttggatcga
     1741 aacaaagacc aaagctattt cctctaccgc ctcaagcctt tccaactcga acgcgcgatt
     1801 tttccgttgg gcggtttgga aaaacccgaa gtgcgccgcc ttgccgccga gttcaaactg
     1861 ccgaccgccg ccaaaaaaga cagcacgggc atctgcttca tcggtgaacg cccgtttcgc
     1921 gagtttctgc aaaaatacct gccgaccgac aacggtaaaa tggttacgcc cgaagggaaa
     1981 accgtcggcg aacacgtcgg gctgatgttc tacacgctcg gacagcgcaa aggtttgggc
     2041 atcggcggcg cgggcgaacc gtggtttgtt gcggctaaag atttgacgaa aaacgaactg
     2101 attgtcgtgc aaggacacga ccatccgctg ctctataccc gcagccttgt gatgaacgat
     2161 ttgagtttca cgcttcccga acgcccgaag gcaggacgct atacctgcaa aacgcgttac
     2221 cgtatggcgg acgcgccttg tgagttgcgc tatttggatg aagaaaccgc cgagctggtg
     2281 tttgacgaac cgcaatgggc ggttacgccg ggccagtctg ccgtgctgta cgacggcgac
     2341 atctgtttgg gcggcggcat tatccaatca accgacaaac ccgtcatcat cacacgataa
     2401 aggtaatgcc gtctgaaacg gttttcagac ggcattgttc cgctcaattc cactttaaag
     2461 accgatacct atggaaaaaa tctggttaga cagttacgaa cagggcgtga atgccgaaat
     2521 cgacatcacg caatacggtt ccgtcagcga tgtattttgc caaagtgtca agaaatttgc
     2581 cggccaacct gcttttcaaa acatgggcaa gacgctgact tatgccgaaa ccggcaaact
     2641 ggcaacagat tttgcttctt atctgcaaaa cgtcctgaag ctgccgcgcg gcgaacgtgt
     2701 tgccattatg ctgcccaacg tattgcaata cccgattgcc cttttcggca ttttgcaggc
     2761 aggtttggta gcggtgaaca ccaatccgct ctataccccg cgcgagctgg agcatcagtt
     2821 gaaagacagc ggcgcgaccg ccatcatcgt tttggaaaat ttcgccaaca cgctggagct
     2881 ggtgctgccg cgcacgcaga tcaaacacgt catcgtagcc tccgtcggcg aaatgttcgg
     2941 gctgcttaaa ggttcgctga tcaatttcgt catccggaaa atcaagaaaa tggtgcccga
     3001 ataccgtatt cgggaaacca tttcctttca gacggcattg aaagaggggg cgaagcacgt
     3061 tttccaacct gtcgcattaa accgcgaaga taccgcgctg ttgcaataca cgggcggcac
     3121 gacaggtgtt gccaaagggg cggtgctgag ccacggcaac atctgcgcca atatgcttca
     3181 ggcaaaagaa tggattaaaa accagttgcg tgagggaaaa gaaaccgtta tcgccgcctt
     3241 gccgctgtac catatttttg ccttaaccgt gaatctgatg atttttgcca atgccggctc
     3301 gaaaattgtc ctgattacca acccgcgcga tatgaaaggc tttatcggag aactgaaaaa
     3361 acagcgggtt aatgtattta tcggcgtgaa cacgctgttt aacgcgatgg ttaaccgtcc
     3421 tgattttgcc gaagtcgatt tttcaggatt gcggctgact ttgggcggcg gtatggcgac
     3481 ccaaaaagcc gttgccgaaa aatggaaaaa aatcaccgac acgcccatcg tcgaagccta
     3541 cggtctgacc gaagccagcc ccggcgtgtg ctgcaacccc ttaaacatcg aatcatacag
     3601 cggcagcatc ggtttgcccg tctcgtccac cgaagtcgaa ctgcgcgacg caaacggcaa
     3661 agaagtcccc gtcgggcagc cgggcgaatt gtgggtgcgc ggcccgcaag tcatgaaagg
     3721 ctattggaac cgtcccgaag aaaccgctaa agccatagac gcgcgcggtt tcttggaaac
     3781 cggcgatatt gccgtcatgg acgaaaaagg ctggttgaag ctggttgacc gtaagaaaga
     3841 cctcattgtt gtttctggtt ttaatgttta tccgaacgaa atcgaggaag tcatcgcgca
     3901 tcacggcaaa gtgatggaag tcgcctgtat cggcgttccc gacgaaaaaa ccggagaagc
     3961 tctcaaagtg tttgtcgtca gaaaagatcc gtctttgacg aaagaagaac ttatcgcctt
     4021 ttgccgcacc gaattaaccg catataaagt accgaaaaat atcgaattcc gcgacgagtt
     4081 gcccaagtcc aacgtcggca aaatcctgcg ccgcgagttg cggcaaagta ctgggaaata
     4141 aagaaaagat gccgtctgaa aacagccgtc caccgttcag acggcatccg tccgtttgcc
     4201 agaaccgcgc gcttcaagtt aaaatcgcac attccaacat gggtattcca tcatgaccaa
     4261 attcattttc gtaaccggcg gcgttgtctc ctcactgggt aaaggtatcg ccgccgcttc
     4321 tattgccgct atcctcgaat cgcgcggctt gaacgttacc atgctcaagc ttgatcctta
     4381 tatcaacgtc gatcccggca cgatgagccc gtttcaacac ggcgaagtgt tcgtaaccga
     4441 cgacggcgcg gaaaccgacc tcgacttagg acactacgaa cggtttatcg attccacgat
     4501 gacccgccgc aacagcttca gcacggggca ggtgtacgaa aacgtcatcg ccaaagaacg
     4561 acggggcgac tacctcggcg gcacggttca agtcatcccg cacattaccg acgaaatcaa
     4621 acgccgcatc cacgaaggcg cggcaggtta cgatgtggcg attgtcgaaa tcggcggcac
     4681 ggtcggcgac atcgaatcgc tgccgttttt ggaagccatc cgccagatgc gaagccagtt
     4741 gggacgcaac aacaccctgt tcgcccactt gagctacgtc ccctacatcg ccgctgcagg
     4801 cgaaatcaaa accaagccga cccagcacac cgttaaagaa atgttgagca tcggtttgca
     4861 acccgacatc ctgatttgcc gtatggacag gacaatgcct gcggacgaac gccgcaaaat
     4921 cgccttgttc tgcaacgtgg aagaacgcgc gattgtcggc agctacgatg tggacagcat
     4981 ctacgaatgc cccgaaatgc tgcacgacca aggcatcgac aacatcatta ccgagcagtt
     5041 gcagcttaat gtgcagcagg cggatttgac cgcgtggaaa aaaatcgtcc acgccatcca
     5101 aaacccgaaa cacaccgtca aaatcgccat ggtcggcaaa tacgtcgatt tgaccgaatc
     5161 ctacaaatca ctgattgaag ccttgaaaca cgcaggtgtt cacaccgaaa ccgacgtaca
     5221 aattaccttt gttgacagcg aaagcatcga gaaaaacaac ggcgacgttt ctatgctcaa
     5281 agatatggac gccatcctcg ttcccggcgg tttcggttcg cgcggcgtgg aaggcaaaat
     5341 cgccgctgtg cgctatgccc gcgaaaacaa cgtgccatac ttgggcatct gcctcggtat
     5401 gcagattgcg ctgatcgaat acgcccgcga cgtggcaggt ttgaaaggtg cgaattccac
     5461 cgagttcgac ctcaaatgcg ccgcccccgt cgttgccctg attgacgaat ggcaaactgc
     5521 cgacggcagc gtcgaaaccc gcgacgaatc cgccgatttg ggcggcacca tgcgtttggg
     5581 cgcgcaagaa gtcgaattga aaacaggcag cctcgccgcc aaatctacgg cagcggacac
     5641 atccgcgaac gccaccgcca ccgctacgaa gtcaacaaca actatgttcc tacgctggaa
     5701 caggcaggtt tggtcatcgg cggcgtatcc gccggacgcg aacgcttggt cgaaaccatc
     5761 gaactgccga accatccttg gttcttcgcc tgccagttcc accccgagtt tacctccaat
     5821 ccgcgcaaag ggcatccttt gttcaccgcg tttgtcaaag ctgcgttgaa caataaaaaa
     5881 gcctgataaa gcgttacttg atgatcaaaa tgccgtctga aagcctgaat gaagactttc
     5941 agacggcatt ttcgcaaatc ggggattata aaagccatca atttaatttt tatggtttta
     6001 tctaatattt accaactgaa tattgttaag cggggcgtga aactcaagtt gagacctttg
     6061 caaaattccc caaaatcccc taaattccca ccaagacatt taggagcacc ttcttccggc
     6121 aaaccgccca agccatgatt gccaaacaca tcgaccgttt cccgctattg aagttggacc
     6181 gggtgattga ttggcagccg atcgaacaat acctgaaccg tcaaaaaacc cgttacctta
     6241 gagaccaccg cggccgtccc gcctacccgc tgttgtccat gttcaaagcc gtcctgctcg
     6301 gacaatggca cagcctctcc gatcccgaac tcgaacacag cctcatcacc cgcatcgact
     6361 tcaacctgtt ttgccgcttt gacgaactga gcatccct
//
LOCUS       DAWHXS010000108         6221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWHXS010000108 DAWHXS010000000
VERSION     DAWHXS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.108"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            95..268
                     /locus_tag="WIE22_001740"
     CDS             95..268
                     /locus_tag="WIE22_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127788.1"
                     /translation="MAWWEFRGFWGILQRSQTVKLAQKWEQEQKKQQIQQKKETEKSP
                     KHKASRDDWEMER"
     gene            complement(350..2437)
                     /locus_tag="WIE22_001741"
     CDS             complement(350..2437)
                     /locus_tag="WIE22_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ4127789.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2440..3705)
                     /locus_tag="WIE22_001742"
     CDS             complement(2440..3705)
                     /locus_tag="WIE22_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ4127790.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3672..3886)
                     /locus_tag="WIE22_001743"
                     /pseudo
     CDS             complement(3672..3886)
                     /locus_tag="WIE22_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4926..5162)
                     /locus_tag="WIE22_001744"
     CDS             complement(4926..5162)
                     /locus_tag="WIE22_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ4127791.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
BASE COUNT         1968 a         1101 c         1087 g         2065 t
ORIGIN      
        1 tgatctagct tcaatagtgg gaagcggtcg atgtgtttgg caatcatggc ttgtgcggtt
       61 tgctgaaaga aggtgctcat ggaaaatccc ctaaatggct tggtgggaat ttaggggatt
      121 ttggggaatt ttgcaaaggt ctcagaccgt gaaattagcc caaaaatggg aacaagagca
      181 gaaaaaacaa caaattcaac agaaaaagga aactgaaaaa tcgccaaaac acaaagccag
      241 tcgtgatgat tgggaaatgg aacgttaaac cgctattttc atgtattcta ttaaattatt
      301 tgaaaaaaga cttcactttt tgttaaagaa aaatgaagtc ttgttctgtt caacctaaat
      361 tcagaatttt atctgccgat tgaattgttt cttggcggtg tgcaaccatt attttcataa
      421 taccaagact ttttaggtta tggttaattt tttgttcatt ttctacatct aaatggctac
      481 ttgcttcgtc taaaaataga attttgggtc gtttatacaa tgcacgagcc aagataactc
      541 tctgcttttg tccacctgat aagatatttc ccatatcgcc aatcaaggtc tcatagccca
      601 ttggcatttt aagtatatcg tcatgtattt gtgccatttt tgcacattgt tcaatgagct
      661 ccatatttgg gctttcatca aaaaatgaaa tattttcccc aatagaacct gcaaaaagga
      721 catcatcttg gcgaacaatc ccgctcaatc ccctaataaa ggatggagaa acttgatata
      781 tatcatgccc attaatacta actgtaccag tttcaggttt taggctacct gttaaaatgt
      841 ttaacaaagt ggacttcccc cgaccagatt gtcctgttaa aacaactgct tcattatctt
      901 taaattccaa attaatgttt tcaaaaagat atggctcatt atcagcatat ctgaatgaga
      961 cgttttcaac tttaagaacc agttgttcat tatctaattt aggtatatga ttatacttaa
     1021 taatttcagt ttctgtttca tttaaagtaa tgtcagccaa acgttcagca tgaagcccta
     1081 acattttgat ttggatgtat tggtcaacga gagaagctgt tctgctttca aattgccctt
     1141 tataagccaa aaaagccatc agaacaccga ctgtaaatga accatctaaa attgcgcttg
     1201 caccaagata aattatgata atattttcca tgctaaacaa cagtttattt gaaaattcaa
     1261 ataaagcaga gagtttatct gttgtcagct tggtattgac tgtattcaca aataggctca
     1321 tccaagtgcc atgtctttga taatgtttat caaataattt aactgattgg ataccacgaa
     1381 tggtttccat gaaatatgag ttttgtttgg cttcatgaac aatattttct tctgttgcat
     1441 ttcttaatgg gtaatatgca agccaacgaa ttagtatgta caaaacaagt gttaaaagaa
     1501 caatcagcga taattgagtg ctgtaaattg tcattaacac gaaagtaaaa acagccatta
     1561 agctatttaa aactaaaaca aaaaaagtag aagttagtgt ttcttggata tgatctattg
     1621 aaccaaatct tgaaatcaca tctcctaaat gtcgtttact gaaatagtca ttaggtaagt
     1681 caagtaacct tttaaatata ttggctttcc attgtaaatt aagagttgta gataggtgca
     1741 tacctaccca tgcttgtaac aggctaatta actgttgcag gatagccagt aaaccaaatc
     1801 ccaaagtaag ggtcagtaat aaatttttat cagcagttac aatgacatgg tctattaccc
     1861 attgcataaa gaatggacta accaatgcaa agacttccaa agaaatagct aatataagca
     1921 tttgaattaa agagcgtttt aagcctgaaa tcccccttaa tagagataat attttgattt
     1981 tctttgtttc ttttttctct tcaaaatggg tattggggaa taattctagg gcaatccctg
     2041 tgaatttttg tgaaacttcg tccattttga tttttcgcat accgacagca gggtccataa
     2101 tgacgatact gtctttggaa atggaacaaa gtacaacaaa atggtttaag ttccaatgga
     2161 gaatgcaggg tagttgtaaa tttgacagct catctaactc taaacgcaaa gctcgtggcg
     2221 ttaaattcat ttcattgcca aatctcatga tgtctgcaag atttgcgccc tttaatgaca
     2281 gggtgtattt ttggcgtaac gttcttaaat cagtgtgaaa gccataatag gacaagatgg
     2341 atgtcaggca tgctaaacca cattcagcaa cttctgtttg cagaatgaca ggtagctttt
     2401 tgttaaatcc aaaggacagt cttgataaat aatccatttt taattgattt ttcctgaaat
     2461 gctgtaaagt gggtcaagta cccattcgta caattttttt cgttcatgga gaatatctgc
     2521 ttctaaaatc atgccaattt gaagcggctt gttttctccg tatgctttaa tcgtttgttt
     2581 ttccaattta actttcacaa gataggcagg ttcatttaat aaggttgggt tagtgaaaat
     2641 gatacctaaa cctgatagct cttgtttacc gagagcagtt ctggcaactg aaataatttc
     2701 tcctgtggca tgtccaaatt tttggtaagg gtacgcttgg taacgtaaaa caactttatc
     2761 tttcggttta ataaaaccaa cagctttact gggtatgtaa agattggcga ccaattcagt
     2821 ttgttcaggg acaatgctta acagcaattt agacggttca acttgttgcc ctatatcaac
     2881 attaattgtt gatatataac ctgatttact agctcgtatg gtttgttcgg atttcaaatc
     2941 aaaatccaaa atttcttggt tcatttccgt aatcgcacgg ttgagttggc tcaattcggt
     3001 tttatggcgt tcaggcaggc tgcttaatgt aattttctgt tcatcaagtt ccctgattgc
     3061 attattttgt tcaagtttta ggctctccaa acgtgagcgt tgttccaata aatggctttc
     3121 ggcggtcatc ttatcttgtt gggatactgc gccttgactg gctaaaaact tgttcttgtt
     3181 aagggttttt tccgctaaac gaatttgacg attttgccct gtaatttgct gtttaatatt
     3241 ctctaattga ttgtttaaac gatgaatgtt gttatgaaca tttttttgct cattttgatg
     3301 aatgcgcttt aaacgttcca attcttgtaa tgccaaagtt tttttaaggt tggcttctgc
     3361 tgccaatttg gcttgtacgt ttcctttttc gccaaaacgc gatgtggaaa gtttgaacaa
     3421 tggttcgcca gctttgacaa agttaccatc ttcaacaaat ttatgcgtaa tcgtgccgat
     3481 atcggaagag taaacacgaa ccacccccat agttggaagt aattgacctt caacggttgt
     3541 tttattggta tagctaccaa aaatcaaaaa gataatgata cacagagcaa tgagaaaagc
     3601 gcaaaaagtc agaaataaaa aagagaatgg acgggtcaag ataacctgac ctgtccactt
     3661 attttgttgg gctacaaaga cttcttttcg gaataagtgg gacatttatt attatttctt
     3721 aaaaaaatgg atagaaaaac accaataatt aaatacttat gcctaataac ataaatttgc
     3781 tattgattga tgtttctatt ttaattacat taaatttaac caataatcca gaaatggttg
     3841 cagctaaaat gcaaaagaca gtggcgtaaa tggtttcagt attcataatt tttctctttc
     3901 aaaattgttc aatctaaata gaaaaaggta acttcaatac atattgctgt aactgaaatt
     3961 accttttcct taatgatgta attatcttat ggcttcatca tgccacgacc gatattgtat
     4021 ccgccagcag cagagaaaat accagctcca attgcgccaa cccagcctac tggtcctgtc
     4081 cacttgttcc gttgagctac aaagacttct ggtctgaaaa atgagtgatt gttagacatg
     4141 tgtacttatt tatgtttctt tgattgggat aaatttgtaa aaacaaaaaa aaggcaagtt
     4201 gttattatag aagaacttaa aatgcttaac atactcattg aggtttgtac cacataatga
     4261 gatacgagat aatttgttgg gagtaaaatc aaaaaaagat taaatattta aaataatcac
     4321 tatatttttt cattatttgt tcctttttgt atttaagttc ttatgaaaaa ctagggtatt
     4381 ttgaatagaa aatatcaaaa taccctagtt ttattaagga atattaccac caacaagtgc
     4441 ttccatattg tacagcacca gtgattgcac caccaatagc tccgaatttc gcacctggaa
     4501 tagcaccaat accacctgca aatgaaccaa caattgcccc accagctgct ccacctaatg
     4561 cactaccaat ggtatttttt gagaaatcac gccagttaca agcagcacca gaaacttatt
     4621 gaatttcatt caaagtcaaa acttgcataa tattcacctt tgttaagtgt ggttgagaaa
     4681 aaagtaaatt ttcatcgtta ctcattattt gcaatcatgc atatactgta accaagcact
     4741 atattcagtt ggatcatagt ttttcctact gggttgtctg atatgactat tggcactatt
     4801 ggcacaattg ggattagcaa ctttattgtg agtacctaat ggattgatat ttcctgtaaa
     4861 gtggctattg catgcgctta atgagcatag atttattaat gtcagaaagg ataaaacaaa
     4921 aaatcttacc attttttact aataccaaaa gtaggtttcg aaaactgtac accaagacct
     4981 tgtacatatc caaattgctt gctaacagaa aactctactc cactattgtt aggggaatag
     5041 gaagcaccaa ttttaccaac agcaaccata tcttttttac ctaggttgcc gccaccctgt
     5101 gcaaagatat ggaatttgcc acctgacact tcaactaatt cagaggtgtg taactctttc
     5161 atgattttct cctgtaaaga agtatggttg gaaatgtaaa tcagaattaa cctttcttaa
     5221 gtttaattta atactttttt tgcaagtatt ttattttaaa ttaaatcaat attttaaaaa
     5281 tatttacttg tatactgaat atttacttgt atactgaaaa agaaactcct aatggtggag
     5341 tggggctttt gtttaagtaa gcattgaaaa aatttaaatt tcccattatg attaacttaa
     5401 tgcaagagct ttttcaggac gtacaaacca gcgaacgggc gttctgatgt gggcaggttg
     5461 gttgtcgcta accaatttca gtagctcaat cgtcagattg gtatccggcc ctatcaagct
     5521 aaggagaggc tgcggctact acacaaagca cctgaaatta ttgggtgctt ttgtcttttt
     5581 atggcattgt accaatttgt gtaccacgag ggcttgttcg cccaaacgcg gtaacaactg
     5641 aatacacact ttccccctca acttctttgc gttctaaaac gactgtccca atccgagaat
     5701 agacaatcat aggacgatga ttgcccctta catcactaaa ttcactaaaa atttttgcgc
     5761 ctgattgaaa aatcatttcc acataaaagg gttacacctg tttcatcgtg gcttaatcca
     5821 aaccgcgtta aatctgcttt gtgttctgcc aaaatgtgaa tcacaccaaa gccgtgatta
     5881 acctttttgt gttcgccgtg ttttaaaaca atttgacctg ccgagaagtc tgctctattg
     5941 ttcggacaat agggggaata taccaattac tttaactaca tcattttttg acatttttct
     6001 tgcctttcaa tttaaaatcc aaaatcatac tgccataatt tagcaatcca aaaaaattta
     6061 ggcagcagtc atggtttcgt attttagaaa acgaaacttg gtttggtgtt ttgaagcatt
     6121 tcagtacaca aacatggaat tttaaattgt cttgtgttgc cttgatttag gatgttccag
     6181 ttttttaata aatagtttgg ggctgtccta gataactagg a
//
LOCUS       DAWHXS010000109         6191 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWHXS010000109 DAWHXS010000000
VERSION     DAWHXS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.109"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(169..2175)
                     /locus_tag="WIE22_001745"
     CDS             complement(169..2175)
                     /locus_tag="WIE22_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ4127792.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVSVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2464..4491
                     /locus_tag="WIE22_001746"
     CDS             2464..4491
                     /locus_tag="WIE22_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ4127793.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGVLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4582..6135)
                     /locus_tag="WIE22_001747"
     CDS             complement(4582..6135)
                     /locus_tag="WIE22_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ4127794.1"
                     /translation="MNRTYANFYGMLAAACRKNGNGTAVFDGKEKTAYRALKQEVEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASVGLSKELAGLKAQTPIEKIIWTGKSRPVGETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
BASE COUNT         1288 a         1435 c         1873 g         1595 t
ORIGIN      
        1 tttttgctca atattaggaa ggttttaggc aattgaaaat tttttggcgc atttttatgc
       61 gtcaaatttc gttaacagac tatttttgca aaggtctcat ccttttatgg ggatttcccg
      121 catcagggcg gtattcgggg cgaatcccgc ttcagacggc attgccggtc aaggcttgtc
      181 cgaaggagga tgtccctgtt ctttggcggg cgggaggaaa agtatgcagg gattgctttt
      241 aatctgattc caaaaactct taatcagatt gcggatgctg ccgattttcg tgccgcgcga
      301 gcgcagggcg acgaagaggg cgaggctgaa gctgacgcag aggttgacca ggccgatggc
      361 gaggacgctg aaaatgccga gtacgaacgt gcccaaaccg acgttgccgc tgacggcggc
      421 atagccgagg ttggcggagg aaaaggcgac gtggcggatg tccagcggca gcccgaggag
      481 gtgtccgaaa tagccggtca tacccaagag catcccgaag atgaagttgc ctgccaacga
      541 gccgtagtgt ttgtggatgt aggcggcgag gacgcggcgg ggcccggggc gcatgatttt
      601 acgcagcaag gggttgaagg gcaggcgttg gcgcaggttg aggtagtcgg cgcggttgtc
      661 gaaaaaacct gcgatgatgc cggagcagaa cagccacaga cctgcaatgg cggcatacca
      721 cagcgtcggg taagcgatga tgtctatgga tttgaactgg taggcggcgg tgtgggcatc
      781 gagtatgggc tgccggtaca gatgggcata gccgaacgat atggcgcacg ccaaaaggat
      841 ggcgatggaa acgttgccga aaacggcgac actttgggaa cggcatacgt cgatgaggag
      901 cttggcgagt ttgttgtcca ccgctttgcc gccttcgttg agatcgacct gttcggcaaa
      961 gctggcggca gtcatcgcgg gctgtttggt ggcgacggtg cagtgcagca tatggatgat
     1021 cataaagccg atgccgtagt tgaacccagc cgacaaggaa gtcaggaagg ggctgaggcc
     1081 gagtgtgccg atgcggattt tgagcagcgc catcagggcg atgatgatgc cgccgcctgc
     1141 cgccgagtag aacattttga aatattcttt gcggttgcgg gtgatgtagt gttcgccgtg
     1201 gtggcttttg ttttcggtaa tgctgcgggc gagcatacgg atgctgctgc ggcgcaggtg
     1261 gcgggtgctg tattgttcga ccgccgcgta gatgagggag ttcatcagcg cgatggtcag
     1321 gcggttgctg ttgccggcac cggtttggat gtcggtgagc agctttagac ggtctacggt
     1381 ctgccggagc cgttcgagca ggtgggcgac tttgaccgac gaaccggagc cggcgcccgt
     1441 ccctttgcgg cgcaaatagt caatctggct gaaacattgg tcgaacatca cttcgaggtg
     1501 ggcggtgtcg taaggcgtgg tgccgttgcg gtagtgttcg accagtttgg cggtttcgcg
     1561 ttggagggcg acgaaggaag aatcggcttc cagcaggcgc ggggcgatgc ggatgaggtc
     1621 gggttcgatg gcttcggatg ccgtccagac agacagcatt tcgatggcgc gcaaacgcgc
     1681 atcggcaagg cggatgcccg ccgtctgcaa tttggacggc tcggcgtggc tccggatgag
     1741 ttcgtataaa accagccatt ggcggatatt gagcgtttgc agccatttgt cgtcgttttc
     1801 ggaatggaaa agataaagga agacttcgcc caagttggca aaatctttat atgacgggct
     1861 gaagcgttcg tagatgcgta tgcccatttc ccgggcaaag ctgtggcgcg agaagatgcc
     1921 gagtttgatg agtgccggat aaatgtgtat ttgcgcgagc cagatgtaaa aacaccggct
     1981 gaagcgggac agtaattccc tgtcttgttt gaatgtgtcg ataatcaggt cgaaacgttc
     2041 ccccgcacat tttttgccgc cacggcgcaa aaatttaatc agtgcgttga ggacgctgac
     2101 aaaatcggta tgtcccaagc tttccgaaag cagggggcgc aggttttgag gggtaatttt
     2161 cttcatatca gccattgtac cgcatcggca atccggcgtt aaacgccccg atagcgggga
     2221 gggcgcgcct gcggaaggcg gcgcattttc cgccggtttg aaatcaaact atattaatga
     2281 tataataaat atcgataaat caatctgata gaataatccg tattgatttt taattttttc
     2341 gattataaca atttcggccg gtttgtgcat ttcgtcatcc ggaagccggt attttaccgg
     2401 caggttttga ccgctttgcc cgatgaccgg gtttgtctgt ttttaacaaa ggagtgagaa
     2461 ggtttgtccc tgtctgaatt tatagaacgc cgaacgtcat ttaatctgat ggttattttg
     2521 acgactttgt tttttgtgtg tgttttagtg gtattggttt taaccgtgcc ggatcaggtg
     2581 cagatgtggc tcgatcgggc aaaagaagtc atttttaccg agttcagctg gttttatgtt
     2641 ttaacgtttt ccatttttct gggtttcctg ctgatactct cggtcagcag tttgggaaac
     2701 atcaggctcg gacgggatga agatgtgccg gaattcggct tcctgtcgtg gctggcgatg
     2761 ctgtttgcgg ccgggatggg cgtgggtctg atgtttttcg gcgtggcaga gccgttgatg
     2821 cattattttt cggacattac ggccggcacg ccggaacaca ggcagcagca ggcattgctg
     2881 cacacggtgt tccattgggg cgttcacgct tggtcggtgt acggtacgat tgcattggct
     2941 ttggcttatt tcggtttccg ctacaagctg ccgcttgccc tgcgttcttg tttttacccc
     3001 ctgttgaaag aaaaaatttc cggaaggttc ggcgatgcca ttgatattat ggcgttgctt
     3061 gctacttttt tcggcatcat caccacattg gggttcgggg cttcgcaact gggcgccgga
     3121 ttgcaggaaa tgggctggat tgccgaaaac agcttcagcg tgcaggtttt gattatcgcc
     3181 gccgtcatgt ccctcgccgt cgtttcggca atatccggcg tggggaaggg cgtgaaggtg
     3241 ttgagcgagt tgaacctgag ccttgcgttt ttgctgctgt tttttgtttt ggcggcgggt
     3301 cccactgttt acctgttgtc ggcattcggc gacaacatag ggaactacct cggaaatctg
     3361 gtgcgcctca gttttaaaac ttatgcgtac gaacgggaac acaagccgtg gtttgaatct
     3421 tggacggtgc tttattgggc gtggtggtgt tcttgggcgc cgtttgtggg tttgtttatc
     3481 gcgcgcattt caaaggggcg caccatccgc gagtttgtct tcggggtttt gctcatcccc
     3541 ggcctgttcg gcgttttgtg gtttaccgtc ttcggcaata cggcgatttg gctgaatgac
     3601 ggggttgcgg ggggagtgct cgaaaagatg acctcctctc cggaaacgtt gctttttaaa
     3661 ttctttaatt acctccccct gcccgaattg acgagcatcg tcagcctgct ggtcatttct
     3721 ctgttttttg taacttctgc cgattccggg atttatgtcc tgaacaatat tacctctcgg
     3781 gacaaaggct tgagcgcgcc acggtggcag gcggttatgt ggggcgtgct gatgtctgcc
     3841 gttgccgttt tgctgatgcg ctcgggcgga ctcggcaacc tgcagtctat gaccctgatt
     3901 gtttccctgc cgtttgccct gctgatgctg ataatgtgtt tcagcctgtg gaaaggattg
     3961 agtgcggata agaaatattt tgagacccgg gttaacccta ccagtgtatt ttggacgggc
     4021 ggcaagtgga aagaacggct ggtgcagata atgagccaga cgcaggagca ggatatttta
     4081 aaattcctca aacataccgc atcgcccgct atgcacgagt tgcaacggga gctttcggaa
     4141 gaatacggct tgagcgtccg ggtcgataag atgtttcatc aggacgagcc cgcaatcgag
     4201 ttcgtcattc ggaaagagac gatgcgcgat tttatgtacg ggattaagtc tgtcgggcag
     4261 gatgtatccg accagttgat taacgacggc aagctgccgc atatccggca tcagacaact
     4321 tacaaaccct acgcttattt tttcgacggg cgcgtcgggt acgatgtgca gtatatgaac
     4381 aaggacgagc tgattgccga cattttgaaa aactacgaac gttatttgat gttgttggat
     4441 gatgtcggtc aggaactgat ggcgcacgag caggtggaat tggcagagta agtgccttat
     4501 ctccggcgtt tgccaaaaaa aatgcggtga atcagattca ccgcattttt ttgccggacg
     4561 gggcttcgga cggcacggcg ttcatttgtt tccgtcaaac tgctccttca acacccgttt
     4621 caataccttg cccgtagcgt tgcgcggcag cccgtcttta aagtggattt gtttggggat
     4681 tttgaaattt gccagcacgg tacgcaggtg gcggcggatt tcgttctcgc ccaaatccat
     4741 accttccttc aattggacga aggcgacgat ttcctcgtcg gcataacggt ctttcacgcc
     4801 gatgacggcg gcggcttcga cggcatcgag tttgtagatt tcttcttcaa tctcgcgcgg
     4861 atagacattt tgacctttgg aaataatcaa atcttttttg cggtcgacga taaagataaa
     4921 gccgtcttcg tctatggtaa cgaaatcgcc cgttttcaac cagccgttga cgatggtttc
     4981 atcggtggcg gcaggcatat tgaggtagcc ccgcatcacc gaaccgcccc tgacgatcag
     5041 ttcgcccact tcgccgcgcg gcacttcgac caattcttca tcgacggctt tggcttccaa
     5101 accgggcagg gggatgccga cgctgcgggc tttttgcctc tcgggcgtat tgacggcgac
     5161 gacgggagag gcttcgctca gtccgtagcc ttccagcaat ttggcgcggg ggaacttggc
     5221 tttgaaatcg aggatggttt gttccgccaa aggcgcgccg ccgctgataa acaggcgaat
     5281 gcggttgaac catctgaaat accaagggat tttcgccttg ctcatcgcgg tgtaaatcgc
     5341 gggtatgccc aaaaacacgg tcgcgcgttt gagcagggtc tgtttcaaaa cattggaaaa
     5401 cgggaaaacg gatttgacca aaataatcga acacgccata taaatcggca gcagcaccat
     5461 agccgtcagc gtgaagctgt ggaacatcgg caggaaaacg ataaagcggt cgcgtttgga
     5521 aattttaaag atgcgttcga tgccgttcag gttggcgaac aggttggcgt aactgattag
     5581 tgcgcctttg ggatgccccg tcgtgccgga ggtgtagatg atgtgtgcca aatcatttat
     5641 ccggggttgt cggcccaagt cgggtttttc ggggaagcgg cgcacgtctt caaaaaaggc
     5701 atcgccttcc gccgtttcgc cgaccggccg gcttttgccc gtccaaatga ttttttcgat
     5761 gggcgtttgc gccttcaagc ccgccaattc ttttgacagg ccgaccgagg cgaacaggaa
     5821 gcgcgccttg cagtcgttca ggatatacgc gtattcgctg tttttcaaaa atgtgttcat
     5881 cggtacggcg accgcgccga tggcggagat ggcgaaatag gcggtaataa attctgtgga
     5941 attggaaacc gccagcgcga ccgtgtcgcc gaacttcacg ccgatatttt gcagatacgc
     6001 cgcgacggct tcgacctcct gcttgagcgc gcggtaggcg gttttttcct tgccgtcgaa
     6061 cactgccgtg ccgttgccgt ttttgcggca ggcggcggcg agcattccgt agaaattggc
     6121 ataagtccgg ttcatgaaac ttcctttatg gttgcggacg gcgaacgccc tcatttgaaa
     6181 acaatatgcc g
//
LOCUS       DAWHXS010000110         6094 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWHXS010000110 DAWHXS010000000
VERSION     DAWHXS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.110"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            637..2781
                     /locus_tag="WIE22_001748"
     CDS             637..2781
                     /locus_tag="WIE22_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ4127795.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKTASPKDQGGYGFAMRLKRRNWYPSAKENEVKLNEGDWEATGLPTEPKKLP
                     LKQESVISKVEANNGDNNIYTSPYLTQSNHQNGSANQPKNEVKDYKEFKYVYSGWFYK
                     HAKLEIIKENNLIKGAKSGDDGYIFYHGEKPSRQLPVSGEVTYKGVWHFVTDTKQGQK
                     FNDILETSKGQGDKYSGFSGDEGETTSNRTDSNLNGNHEGYGFTSNLEVDFDNKKLTG
                     KLIRNNRVTNATTGDKHTTQYYSLEAQVTGNRFNGKAMATDKPGNGETKQHPFVSDSS
                     SLSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDKDANGNTEAASGGTGAAASGGAA
                     AMPSENGKLTTVLDAVELKSGGKEVKNLDNFSNAAQLVVDGIMIPLLPKDSESGNNQA
                     NQGTNGGTAFTRKFDHTPNSDEKDTQAGTAANGDQAASNTAGDTNGKTKTYAVEVCCS
                     NLNYLKYGMLTRKNSKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPSEQNIV
                     YRGSWYGHIASSTSWSGNASDKEGGNRAEFTVNFGEKKITGTLTAENRQEATFTIDGK
                     IEGNGFSGTAKTAELGFDLDQKNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYQGD
                     KQTENTTVASGNGNSASSATVVFGAKRQKPVQ"
     gene            2867..5608
                     /gene="tbpA"
                     /locus_tag="WIE22_001749"
     CDS             2867..5608
                     /gene="tbpA"
                     /locus_tag="WIE22_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ4127796.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDDHKYAHFVVKEECESGSYETCKSKP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGDHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNANRAYSLKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTGQICRLGNNT
                     YTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSW
                     NAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVF
                     KGDFGNLEASWFNNAYRDLIVRGYEAQIKDGKEEAKGDPAYLNAQSARITGINVLGKI
                     DWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQP
                     EGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKK
                     HFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
BASE COUNT         1748 a         1503 c         1594 g         1249 t
ORIGIN      
        1 ctctctcccg tgggagagag tcagagagag gacgacaagc cgcaaggctt gtattttaga
       61 agactaaggg atttgggaaa gattgccgca acttggaaaa tgccctctcc ccaaccctcc
      121 cctgcagggg agggggagga ttgcggcaga tttggcggtt gcaggcgatt tgaaaagcaa
      181 cttggattta caaccgttga tgcaggtcgt ctgaaaaaga aaaaaacagc ctgcacaagc
      241 tgattagttt gcataaacct tagccctaac agccgcaccg tcctctctcc cgtgggagag
      301 agttagagag agggcagcaa gccgttttag aagactaagg gatttgggaa aggttgccgc
      361 gattcggaga atgccctctc cccagccctc ccccgcaggg gagggagcgg attgcggcag
      421 ccctcccccg caggggaggg agcggattgc ggcagacctc ccccacgggg gagggagcgg
      481 attgcggcag attttgcggt tgcaggcggt tttaaaagca gcgcgggaat gacgggattt
      541 tggtgtattc cctaaaaaaa taaaaaagca tttgcaaatt tgttaaaaat aaataaaata
      601 ataatcttta tcattcttta attgaattgg gtttatatga acaatccatt ggtgaatcag
      661 gctgctatgg tgctgccggt gtttttgttg agtgcttgtc tgggcggagg cggcggcagt
      721 ttcgatcttg attctgtcga taccgaagcc ccgcgtcccg cgccaaagta tcaagatgtt
      781 tcttccgaaa aaaccgcaag cccaaaagac caaggcggat acggttttgc gatgaggttg
      841 aaacggagga attggtatcc gtcggcaaaa gaaaacgagg ttaaactgaa cgagggtgat
      901 tgggaggcga caggattgcc gacagaaccc aagaaactgc cattaaaaca agaatccgtc
      961 atttcaaaag tagaagcaaa caatggcgac aacaatattt acacttcccc ctatctcacg
     1021 caatcaaacc atcaaaacgg cagtgcaaac caaccaaaaa atgaagtaaa agattataaa
     1081 gagttcaaat atgtttattc cggttggttt tacaaacacg ctaaactcga aatcataaaa
     1141 gaaaacaact taattaaggg tgcaaagagc ggcgacgacg gttatatctt ttatcacggt
     1201 gaaaaacctt cccgacaact tcccgtttct ggagaagtta cctacaaagg tgtgtggcat
     1261 tttgtaaccg atacgaaaca gggacaaaaa tttaacgata ttcttgaaac ctcaaaaggg
     1321 caaggcgaca aatatagcgg cttttcgggc gatgaaggcg aaacaacttc caatagaact
     1381 gattccaacc ttaatgggaa tcacgagggt tatggtttta cctcgaattt agaagtggat
     1441 ttcgacaata agaaattgac gggcaaactg atacgcaata atagagttac aaacgctact
     1501 actggcgaca aacataccac gcaatattac agccttgagg ctcaagtaac aggcaaccgc
     1561 ttcaacggca aggcgatggc aaccgacaaa cccggcaacg gcgaaaccaa acaacatccc
     1621 tttgtttccg actcgtcttc tttgagcggc ggctttttcg gcccgaaggg tgaggaattg
     1681 ggtttccgct ttttgagcga cgataaaaaa gttgcggttg tcggcagcgc gaaaaccaaa
     1741 gacaaagacg caaatggcaa tactgaggcg gcttcaggcg gcacaggtgc ggcagcatcg
     1801 ggcggtgcgg cagctatgcc gtctgaaaac ggtaagctga ccacggtttt ggatgcggtc
     1861 gagctgaaat cgggcggtaa ggaagtcaaa aatctcgaca acttcagcaa cgccgcccaa
     1921 ctggttgtcg acggcattat gattccgctc ctgcccaagg attccgaaag cgggaacaat
     1981 caagccaatc aaggtacaaa cggcggaaca gcctttaccc gcaaatttga ccacacgccg
     2041 aacagcgatg aaaaagacac ccaagcaggt acggcggcga atggcgatca agccgcttca
     2101 aatacggcag gtgataccaa tggcaaaaca aaaacctatg cagtcgaagt ctgctgttcc
     2161 aacctcaatt atctgaaata cggaatgttg acgcgcaaaa acagcaagtc cgcgatgcag
     2221 gcaggagaaa gcagtagtca agctgatgct aaaacggaac aagttgaaca aagtatgttc
     2281 ctccaaggcg agcgcaccga tgaaaaagag attccaagcg agcaaaacat cgtttatcgg
     2341 gggtcttggt acgggcatat tgccagcagc acaagctgga gcggcaatgc ttctgataaa
     2401 gagggcggca acagggcgga atttactgtg aattttggcg agaaaaaaat taccggcacg
     2461 ttaaccgctg aaaacaggca ggaggcaacc tttaccattg atggtaagat tgagggcaac
     2521 ggtttttccg gtacggcaaa aactgctgaa ttaggttttg atctcgatca aaaaaatacc
     2581 acccgcacgc ctaaggcata tatcacagat gccaaggtgc agggcggttt ttacgggccc
     2641 aaagccgaag agttgggcgg atggtttgcc tatcagggcg ataaacaaac ggaaaataca
     2701 acagttgcat ccggcaatgg aaattcagca agcagtgcaa ctgtcgtatt cggtgcgaaa
     2761 cgccaaaagc ctgtgcaata agcacggttg ccgaacaatc aagaataagg cttcgacggc
     2821 atcgctcctt ccgataccgt ctgaaagcga agattaggga aacattatgc aacagcaaca
     2881 tttgttccga ttaaatattt tatgcctgtc tttaatgact gcgctgcccg tttatgcaga
     2941 aaatgtgcaa gccggacaag cacaggaaaa acagttggat accatacagg taaaagccaa
     3001 aaaacagaaa acccgccgcg ataacgaagt aaccggcctg ggcaaattgg tcaaaaccgc
     3061 cgacaccctc agcaaggaac aggtactcga tatccgcgac ctgacgcgtt acgaccccgg
     3121 catcgccgtc gtcgaacagg ggcgcggcgc aagctcgggc tactcgatac gcggtatgga
     3181 taaaaaccgc gtttccttga cggtggacgg cttggcgcaa atacagtcct acaccgcgca
     3241 ggcggcattg ggcgggacga ggacggcggg cagcagcggc gcaatcaatg aaatcgagta
     3301 tgaaaacgtc aaagctgtcg aaatcagcaa aggctcaaac tcggtcgaac aaggcagcgg
     3361 cgcattggcg ggttcggtcg catttcaaac caaaaccgcc gacgatgtta tcggggaagg
     3421 caggcagtgg ggcattcaga gtaaaaccgc ctattccggc aaaaaccggg ggcttaccca
     3481 atccatcgcg ctggcggggc gcatcggcgg tgcggaggct ttgctgatcc acacgggtcg
     3541 gcgcgcgggg gaaatccgcg cccacgaaga tgccggacgc ggcgttcaga gctttaacag
     3601 gctggtgccg gttgaggacg accacaaata cgctcatttc gtggtcaaag aagaatgcga
     3661 aagcgggagt tatgaaacgt gtaaaagcaa accgaaaaaa gatgttgtcg gcaaagacga
     3721 acgtcaaacg gtttccaccc gagactacac gggcccaaac cgcttcctcg ccgatccgct
     3781 ttcatacgaa agccgatcgt ggctgttccg cccgggtttt cgttttgaaa acaaacggca
     3841 ctacatcggc ggcatactcg aacacacgca acaaactttc gacacgcgcg atatgacggt
     3901 tccggcattc ctgaccaagg cggtttttga tgaaaataga aaatacggac cgataagagg
     3961 caacggcaaa tacgcgggcg accacaaata cggcggtctg tttaccaacg gcgaaaacgg
     4021 tgcgctggtg ggcgcggaat acggtacggg cgtgttttac gacgagacgc acaccaaaag
     4081 ccgctacggt ttggaatatg tctataccaa tgccgataaa gacacttggg cggattatgc
     4141 ccgcctctct tatgaccggc agggtatcgg tttggataat cattttcagc agacgcactg
     4201 ttctgccgac ggttcggaca aaaattgccg tccgagtgcc gacaagccgt tttcctatta
     4261 caaatccgac cgcgtgattt acggggaaag ccacaggctc ttgcaggcgg cattcaaaaa
     4321 atccttcgat accgccaaaa tccgccacaa cctgagcgtt aacctcggtt ttgaccgctt
     4381 tggctctaat ctccgccatc aggattatta ttttcaaaat gccaaccgcg cctattcgtt
     4441 gaaaacgccc cctaaaaacg gcaatcaaaa cagccccaac ggcgacaaga gcaaacccta
     4501 ttgggtcagc ataggcgggg gaaatgtggt tacggggcaa atctgccgct tgggcaacaa
     4561 tacttatacg gactgcacgc cgcgcagcat caacggcaaa agctattacg cggcagttcg
     4621 ggacaatgtc cgtttgggca ggtgggcgga tgtcggcgcg gggttgcgct acgactaccg
     4681 cagcacgcat tcggacgacg gcagcgtttc caccggcacg caccgcaccc tgtcctggaa
     4741 cgcaggcatc gtcctcaaac ctgccgactg gctggatttg acttaccgca cttcaaccgg
     4801 cttccgcctg ccctcgtttg cggaaatgta cggctggcgg gcgggtgttc aaagcaaggc
     4861 ggtcaaaatc gatccggaaa aatcgttcaa caaagaagcc ggcatcgtgt ttaaaggcga
     4921 tttcggcaac ttggaggcaa gttggttcaa caatgcctac cgcgatttga ttgtccgggg
     4981 ttatgaagcg caaattaaag acggcaaaga agaagccaaa ggcgacccgg cttacctcaa
     5041 tgcccaaagc gcgcggatta ccggcatcaa tgttttgggc aaaatcgatt ggaacggcgt
     5101 atgggataaa ttgcccgaag gttggtattc tacatttgcc tataatcgtg tccatgtccg
     5161 cgacatcaaa aaacgcgcag accgcaccga tattcaatca cacctgtttg atgccatcca
     5221 accctcgcgc tatgtcgtcg gcttgggcta tgaccaaccg gaaggcaaat ggggtgtgaa
     5281 cggtatgctg acttattcca aagccaagga aatcacagag ttgttgggca gccgggcttt
     5341 gctcaacggc aacagccgca atacaaaagc caccgcgcgc cgtacccgcc cttggtatat
     5401 tgtggatgtg tccggttatt acacgattaa aaaacacttt accctccgtg cgggcgtgta
     5461 caacctcctc aactaccgct atgttacttg ggaaaatgtg cggcaaactg ccggcggcgc
     5521 agtcaatcaa cacaaaaatg tcggcgttta caaccgatat gccgcccccg gtcgcaacta
     5581 cacatttagc ttggaaatga agttctaaac gtccaaactc cgcaaatgcc gtctgaaagg
     5641 cttcagacgg cattttttac acaatcccca ccgtttccca tcatccccga tacaccgtaa
     5701 tcctgaaatc cgtcattccc gagcaggcgg gaatccagtc cgttcggttt aggttttttt
     5761 gaggtttcgg gtaacttcta aaccgtcatt cccgcgaagg cggaaaatca aaaacagaaa
     5821 cctcaaatcc cgtcattccc gcgcaggcgg gaatctaggg cgtaaaatct aaagaaaccg
     5881 ttttatccga taagtttccg caccgacaga ctagattccc gcctgcgcgg gaatgacgtt
     5941 atatttttcg catttgataa aaaagaccgt ttgaaatttt ttcagcggac gcaaagtatt
     6001 gcgtaaaatg ctgcttataa gaaacgcaag gcgggcgtag gatgtgcagc gtggacatag
     6061 ggctggctgt agggtgggct tcagcccacc aatc
//
LOCUS       DAWHXS010000111         6052 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWHXS010000111 DAWHXS010000000
VERSION     DAWHXS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.111"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..167)
                     /locus_tag="WIE22_001750"
     CDS             complement(<1..167)
                     /locus_tag="WIE22_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4127797.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRIR
                     YVRDHRGRPAYP"
     gene            complement(391..2523)
                     /locus_tag="WIE22_001751"
                     /pseudo
     CDS             complement(391..2523)
                     /locus_tag="WIE22_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
     gene            complement(3305..3958)
                     /locus_tag="WIE22_001752"
     CDS             complement(3305..3958)
                     /locus_tag="WIE22_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4127798.1"
                     /translation="MKTTHCKLKKKVQKELLRFFVLEVTARSAADILGIQPDSAILFY
                     RTIRMVISHYPALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLHVSGFIHHRINHSKEFADRQ
                     NHINGIENFWNQAKRALRKYNEIDRKSFPLFLKECKFRFNFGTPSRQLKILRDWCGI"
     gene            4295..4729
                     /locus_tag="WIE22_001753"
     CDS             4295..4729
                     /locus_tag="WIE22_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127799.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQPYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            4770..>6052
                     /locus_tag="WIE22_001754"
     CDS             4770..>6052
                     /locus_tag="WIE22_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ4127800.1"
                     /translation="MIGSGDTKQCKKFSADEFAKTIQSNFQSGIKYDGPTLATAGVSN
                     IIDEYLKDAYGGNGLKEAQRNLVAKEIADRIEAELHRQRANYQAQEAAKYSPAHGGVE
                     KSMYKHHADLQKTNTNIANRYSASANEYAAQAKSLGSLPKSVGYAALKNLGNALAALQ
                     MGEALYSNKANNIAKTGSSILGGMLAVAAAGAIASLAGVTLPFWASIAVGASIGLGIS
                     AAWDAFGVGEKLGLDPNVPAFDPFFGWLYDKTGGWEGGIPDWAKEWLGLNRDGKYHQY
                     DPLTLDLDGDGIETVATKGFAGALFDHRNQGIRTATGWVAADDGLLVRDLNGNGIIDN
                     GAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQ
                     TNELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQG"
BASE COUNT         1771 a         1388 c         1362 g         1531 t
ORIGIN      
        1 ggataggcgg gacgaccgcg gtggtctcgg acgtaacgga ttctttgacg gttcaggtat
       61 tgttcgattg gttgccaatc aatcacctgg tccaacttca atagcgggaa gcggtcgatg
      121 tgtttggcaa tcatggcttg tgcggtttgc tgaaagaagg tgctcatgga aaatccccta
      181 aatggcttgg tgggaattta ggggattttg gggaattttg caaaggtctc tagatgagtg
      241 aaaaagaagt gcaggctgcc taaaaagaca gaaaaagtct ttccggcagc ctgcactttg
      301 gtttcatttc agtcagtaaa cccagtaaac gacggtctaa aaacgcagaa cgttacgaaa
      361 aaagcagcct acacgcccat cccccgcctt ctacccgttc tgtaaatcat acagatagcg
      421 gtaatatccg ttcggcttcg ccagcaattc ctgctgtgtt cccgcttcca caatcctgcc
      481 tttatccatg gcaatgatcc ggtgtgccgt tttaacggtg gacagacggt gggcgataat
      541 cagcaccgtc cggttggcgc aaatggcctg catgttctgc ataatcgctc gttcactttc
      601 ataatccagc gcgctggtgg cttcgtcaaa aatcagaatg cgcggattgg tgattaacgc
      661 gcgggcaatc gcaatacgct gccgctgtcc gcccgacaag ccggcccctt gttcgcccac
      721 cacggtgccg tagccttccg gcagctccat aataaactcg tgtgcgcccg ccagtttggc
      781 tgcttcgata atgcgttcca gcggcatacc cgtatccgtc agcgcgatat tgtcgcgtat
      841 gctgcggttg agcagcacat tctcctgcaa gaccacgccg acctgccgcc gcagccaggc
      901 aggagcggcc aaagccaaat cgttgccgtc caccaacacc cgtccctgct ccggtacata
      961 cagacgctgc accaatttgg tgagtgtgga tttgcccgac cccgaacgtc ccacaatccc
     1021 cagcacttcc cccgcccgaa tccgcaggtt caaatcctgc aaaatcagcc tgccgtccgc
     1081 cttatagcgg aaatcgacat gttcgaacgt aatctccccc cggatatcgg gcaaagccaa
     1141 atgcgaagac gcattctcgg tcggcgcatt cagaatatcc cccaaacgcg ccaccgaaat
     1201 ccccacctgc tggaaatcct gccacaactg cgccaaacgg ataacaggcg ccgccaccta
     1261 tcccgagagc atattaaacg caatcagctg ccccaccgtc agcttgccct caattaccag
     1321 ccgtgcgcca atccacaacg tcgccaccgt caccagcttc tgaatcagct gcaccccctg
     1381 ctggccgacc accgccaact tcgttacccg aaatcccgaa gccacataag ccgccaactg
     1441 attgtcccaa cgctgcgtca tctgcggctc caccgccatc gcctttaccg tacccaccgc
     1501 agtgatgctt tctactaaaa acgactggtt gtctgcattg cgcgcgaact tatcgttcag
     1561 acgcgtccgc agtatcggac tgataaatgc cgaccaaaac gcataggcag gcaacgaagc
     1621 caataccacc caagtcagag tggagctgta ataccacatc accgccagaa agataaacga
     1681 aaacgccaaa tccaacaccg aagtcagcgc ctgaccggtc aagaaattgc gaatctgctc
     1741 caattcccgc acccgagcca ccgtatcacc cactcgtctg tgctcgaaat aggataaagg
     1801 cagggaaagc agatgccgga acaaacgcgc gcccaattcc acatcaatac gtgaagtcgt
     1861 atgtgcaaac agatacgtcc gcaaaccgcc caacacaatc tcaaacagcg acaccaccaa
     1921 caaagccacc gacaccacat ccaaagtaga gaatccccga tgtaccagca ccttgtccat
     1981 caccacttgg aaaaacagag gcgtaatcag cgcaaacagc tgcaacacca ccgacaccac
     2041 caatacttca aaaaacaacc ggcggtattt gattaccgcc ggaataaacc aggtaaagtc
     2101 aaactttgcc aaactgccca ataccgaagc gcgggaagca accaatatca gtttgcccga
     2161 atatctgtta gaaaattcgg caaaagacaa taccgcagac ttattcgtaa ccaaatcctg
     2221 tatcaaaaat tgggcatgct caccctcacc gtctgttttg gccaaaatga aatggttgcc
     2281 gtcatcacac cataccaatg cgggtaaagt cgccatagcc aaacgtttaa taggctggcg
     2341 gactaccttt gccttcaatc ccaaagattt ggcggctaac agccattgcg tttcatttaa
     2401 atcgctctgt gcggaagtac aaaattcatg ctgtatatcg gcaggattgg cggcaatgcc
     2461 gtggtaatgg gcgaggatga tgagggcgga aagggcgggg agcggtgcgg atacgataga
     2521 cataaataaa atatagttag attggatgtg gataacggct ggctggaaaa ggaatatatt
     2581 aagtagaaag aaatatataa ataaaacagc agaacgcatt gtaaggatat atatgggaat
     2641 tgtaaagaga aagtatggaa aagttctcgt ttcaggaagg taaaacggct taggaatcga
     2701 gttagatgag gatgcctcgc acctctcgtg cctcctgcat accgttaagg aacagggtta
     2761 aggtgcaggc tgctccgaac tctgttgcgg tcggataatg ttattttttg tgtttcagtc
     2821 agcctgaaat atctgtatat ttttgtttta aatagatttt aaagattgat aactgttctt
     2881 gacgattttt caagaaagga gtaaagtgac ttattatcaa tgacaagcaa cgcgcgaagt
     2941 gacaaggaaa actatctact taaattctaa ggaggcttcg aatatcataa accaatcaga
     3001 aacatagaga taaaaattat gtacaaatat aatcctctta tacaatttat tgcacagttg
     3061 attatgtctt atggagcaag catagggtgg gcacttgctg cccacgcgtt tcatatttca
     3121 aggcagcctg aaactgtgtg ggcataatgc ctaccctaca tcccaaaaaa acaagcgcag
     3181 cctgcgtgtg tagggtgcga actttcggca ggcagacacg cggttttata gtttcaagct
     3241 gagttgggat gcttaagata aagtacaaaa ccttaaaaaa taagggggct gtactagatt
     3301 agccctaaat tccacaccaa tcccgcaaaa tttttagctg tcgggacggt gtgccgaagt
     3361 taaaccgaaa tttgcattct ttcaagaaca gcgggaaaga tttacgatca atttcgttgt
     3421 atttgcgcaa ggcgcgtttc gcctgattcc aaaagttttc aatgccgtta atgtggttct
     3481 gacggtctgc aaactcctta gaatggttga tgcggtgatg gataaaaccg cttacgtgca
     3541 gcttgtcata gctgctcaga ctatcggtat aaacaatact gtccggcatg attttcttct
     3601 tgatgacagg gagtaacgtt tcagacttgg cattatccac cacaacggta tagacccgtc
     3661 cgttgcgttt cagaatgccg aagacaacca cttttcctgc tgcaccgcga ccacgtctac
     3721 ctttacgccg tccgccgaaa tcgctttcgt ccggctcgac agggccctca aaaacctcat
     3781 cggcagccaa ggccggataa tggctgatga ccatacggat tgtgcggtag aacagaatag
     3841 cggagtcggg ctggatgccc aaaatatcgg cagctgaacg ggcggtaact tcaagtacaa
     3901 aaaagcggag cagttctttc tgtacttttt tctttaattt gcagtgtgtt gtcttcatat
     3961 ttcgagggta acatatctgc taatctagta cagcctcaaa caaaaaagag aaattttaat
     4021 ttcgctaaat cgcataaaga ttaatcaaga gtatcattaa atgatatgag tgagcatcta
     4081 taatgccaag aagagttgtt aagacataat gattattgaa atagattgta aaatagatac
     4141 ttagatagtc tgaaaaacgg atttgtgaaa ctttttatta cgcgccatca tttgaaaatg
     4201 aaacttaaaa aacacttatc ataataaata ttttctttac gttgtttgct aataaactca
     4261 gtgcaatatc atcgcaatat tttatggaaa ttttatggat aacaaaaaag aatttattaa
     4321 taatttaaca aataggtata tgtggatcta tccattggtc ttaaatattc tatttctacc
     4381 tttttaccag ccctaccaat ctttttttat tgcgcttggt tgtttgtttg cactggttag
     4441 aaaaatgcaa agcttagatt ttaaattaca aaatcatatt gtattgttaa atataaaaag
     4501 tgcttgggca gataaaaaag tatttttgat taggatagta gtgtcatggt tggcggtaat
     4561 ggaaatatgg atgtgtttta tttcggaatc atcaacgtgg gtatgcggtg ctttttgttt
     4621 aaatagtgaa atattggaaa aaatttttcg tggctttggt tattctggta gtttatattt
     4681 tttatttata ttgatgattg atctcaataa gttaagagag agtatttgaa tttcattttt
     4741 tgtttgactt aaactcaagg agaataacaa tgattggtag tggtgatact aaacaatgca
     4801 aaaaattttc tgcggatgag tttgcaaaaa caatccaaag caactttcaa tcaggtatta
     4861 aatatgatgg gccgactttg gcaacggcag gcgtatctaa tataatagat gaatacttaa
     4921 aggatgctta tggcggaaat gggttaaaag aagcgcaacg caatcttgta gctaaagaga
     4981 ttgcggatag aatagaggca gaattgcata ggcagcgtgc taattatcaa gcacaagaag
     5041 ctgcaaaata ttctcctgcg catggcggag tggagaaaag tatgtacaag catcatgctg
     5101 atcttcaaaa aacaaatact aatattgcta atcgttattc tgcaagtgct aatgaatatg
     5161 cggctcaagc aaaatcatta ggaagtttgc caaaatcagt tggctatgct gcactaaaaa
     5221 atttgggaaa tgctttggcg gcgttgcaaa tgggagaagc tctatactca aataaagcta
     5281 ataatatagc caaaacaggc tcttctattt taggtggtat gttagcggtt gcagcagcag
     5341 gtgctatagc tagtttagca ggagttacat tgcctttttg ggcttctatt gcagttggtg
     5401 catctattgg tttaggtatt agtgcagcat gggatgcttt tggtgttggg gaaaaattag
     5461 gattagaccc taatgtgcca gcttttgatc cattcttcgg ttggctatac gataaaacag
     5521 gcggctggga aggaggtatt cctgattggg ctaaagaatg gttgggatta aaccgcgatg
     5581 gcaaatacca ccaatacgac ccactaaccc tagacttaga cggagacggc atagaaaccg
     5641 ttgccaccaa aggctttgca ggtgcattgt tcgaccaccg caatcaaggc atccgcaccg
     5701 ccaccggttg ggttgctgcc gatgatggtt tgcttgtgcg cgatttgaac ggcaacggca
     5761 tcatcgacaa cggtgcggaa ctcttcggcg acaacaccaa actggcagac ggttcttttg
     5821 ccaaacacgg ctacgcggct ttggccgaat tggattcaaa cggcgacaac atcatcaacg
     5881 cggcagacgc cgcattccaa accctgcgtg tatggcagga tctcaaccag gacggcattt
     5941 cccaaaccaa cgaactccgc acccttgaag aattgggtat tcaatctttg gatctcgcct
     6001 ataaagatgt aaataaaaat ctcggtaacg gtaacacttt ggctcagcaa gg
//
LOCUS       DAWHXS010000112         5992 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWHXS010000112 DAWHXS010000000
VERSION     DAWHXS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.112"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..413)
                     /locus_tag="WIE22_001755"
     CDS             complement(<1..413)
                     /locus_tag="WIE22_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ4127801.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGA"
     gene            complement(449..562)
                     /gene="rrf"
                     /locus_tag="WIE22_001756"
     rRNA            complement(449..562)
                     /gene="rrf"
                     /locus_tag="WIE22_001756"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(659..3550)
                     /locus_tag="WIE22_001757"
     rRNA            complement(659..3550)
                     /locus_tag="WIE22_001757"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3956..4031)
                     /locus_tag="WIE22_001758"
     tRNA            complement(3956..4031)
                     /locus_tag="WIE22_001758"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3996..3998),aa:Ala,seq:tgc)
     gene            complement(4037..4113)
                     /locus_tag="WIE22_001759"
     tRNA            complement(4037..4113)
                     /locus_tag="WIE22_001759"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4077..4079),aa:Ile,seq:gat)
     gene            complement(4215..5755)
                     /locus_tag="WIE22_001760"
     rRNA            complement(4215..5755)
                     /locus_tag="WIE22_001760"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1304 a         1758 c         1283 g         1647 t
ORIGIN      
        1 gcgccgaccg aagcctgatc cttcagcttc gccagcaccg cagggccgat gccctttacc
       61 ttggtcaaat cgtccacaga cttgaacgca ccgttttgcg cacggtattc cgcaatggcc
      121 ttcgccttcg cagggcctat gcccggcagc gcctccaact cctgctgcga agccgcattg
      181 atgtttaccg ccgcaaggga gaaggcgcag gagaacagca tacagaacag cacgaacatt
      241 ttcttcatgg tttttccttt aagggttgca aacaataaac cgcatcttgc gacgataaaa
      301 cgagtcattc taaaatgaat atcccaaagt ttcaagccgt tcctccgcaa acccgaccgg
      361 acgccgtacg gatactgtcc cgccatcccc gacatttttt ccgggcaaag caaaaacccc
      421 cgaataatcg ggggttttct gaaagggtgt ttggcagtga cctactttcg catggaagaa
      481 ccacactatc atcggcgctg agtcgtttca cggtcctgtt cgggatggga aggcgtggga
      541 ccaactcgct atggccgcca aacttaaact gttacaaatc ggtaaagcct taatcaatat
      601 attcggtaat gactgaatca gtcagtaagc ttttatctct tgaagttctt caaatgatag
      661 agtcaagcct cacgagcaat tagtatgggt tagcttcacg cgttaccgcg cttccacacc
      721 ccacctatca acgtcctggt ctcgaacgac tctttagtgc ggttaaaccg caagggaagt
      781 ctcatcttca ggcgagtttc gcgcttagat gctttcagcg cttatctctt ccgaacttag
      841 ctacccggct atgcaactgg cgttacaacc ggtacaccag aggttcgtcc actccggtcc
      901 tctcgtacta ggagcagccc ccgtcaaact tccaacgccc actgcagata gggaccaaac
      961 tgtctcacga cgttttaaac ccagctcacg taccacttta aatggcgaac agccataccc
     1021 ttgggaccga ctacagcccc aggatgtgat gagccgacat cgaggtgcca aactccgccg
     1081 tcgatatgaa ctcttgggcg gaatcagcct gttatccccg gagtaccttt tatccgttga
     1141 gcgatggccc ttccatacag aaccaccgga tcactatgtc ctgctttcgc acctgctcga
     1201 cttgtcggtc tcgcagttaa gctacctttt gccattgcac tatcagtccg atttccgacc
     1261 ggacctaggt aaccttcgaa ctcctccgtt acgctttggg aggagaccgc cccagtcaaa
     1321 ctgcctacca tgcacggtcc ccgacccgga tgacgggtct gggttagaac ctcaaagaca
     1381 ccagggtggt atttcaagga cggctccaca gagactggcg tctctgcttc aaagcctccc
     1441 acctatccta cacaagtgac ttcaaagtcc aatgcaaagc tacagtaaag gttcacgggg
     1501 tctttccgtc tagcagcggg tagattgcat cttcacaacc acttcaactt cgctgagtct
     1561 caggaggaga cagtgtggcc atcgttacgc cattcgtgcg ggtcggaact tacccgacaa
     1621 ggaatttcgc taccttagga ccgttatagt tacggccgcc gtttactggg gcttcgatcc
     1681 gatgctctca catcttcaat taaccttcca gcaccgggca ggcgtcacac cctatacgtc
     1741 cactttcgtg ttagcagagt gctgtgtttt taataaacag tcgcagccac ctattctctg
     1801 cgaccctcca gggcttacgg agcaagtcct taaccttaga gggcatacct tctcccgaag
     1861 ttacggtatc aatttgccga gttccttctc ctgagttctc tcaagcgcct tagaattctc
     1921 atcctgccca cctgtgtcgg tttgcggtac ggttcaattc aaactgaagc ttagtggctt
     1981 ttcctggaag cgtggtatcg gttacttcat gtccgtagac actcgtcatc acttctcggt
     2041 gttaagaaga cccggatttg cctaagtctt ccacctaccg gcttaaacaa gctattccaa
     2101 cagcttgcca acctaacctt ctccgtcccc acatcgcatt tgaatcaagt acaggaatat
     2161 taacctgttt cccatcgact acgcatttct gcctcgcctt aggggccgac tcaccctacg
     2221 ccgatgaacg ttgcgtagga aaccttgggc tttcggcgag cgggcttttc acccgcttta
     2281 tcgctactca tgtcaacatt cgcacttctg atacctccag cacactttac aatgcacctt
     2341 catcggccta cagaacgctc ccctaccatg ccagtaaact ggcatccgca gcttcggtta
     2401 tagatttgag ccccgttaca tcttccgcgc aggacgactc gaccagtgag ctattacgct
     2461 ttctttaaat gatggctgct tctaagccaa catcctggct gtctgggcct tcccacttcg
     2521 tttaccactt aatctatcat tggggacctt agctggcggt ctgggttgtt tccctcttga
     2581 caacggacgt tagcacccgc tgtctgtctc ccgaggaacc acttgatggt attctgagtt
     2641 tgccatgggt tggtaagttg caataacccc ctagccataa cagtgcttta cccccatcag
     2701 tgtcttgctc gaggcactac ctaaatagtt ttcggggaga accagctatc tccgagtttg
     2761 tttagccttt cacccctatc cacagctcat ccccgcattt tgcaacatgc gtgggttcgg
     2821 tcctccagta cctgttacgg caccttcaac ctggccatgg atagatcact cggtttcggg
     2881 tctacaccca gcaactcatc gccctattaa gactcggttt ccctacgcct cccctattcg
     2941 gttaagctcg ctactgaatg taagtcgttg acccattata caaaaggtac gcagtcacac
     3001 ctctagggcg ctcccactgt ttgtatgcat caggtttcag gttctgtttc actcccctcc
     3061 cggggttctt ttcgcctttc cctcacggta ctggttcact atcggtcgat gatgagtatt
     3121 tagccttgga ggatggtccc cccatattca gacaggattt cacgtgtccc gccctacttt
     3181 tcgtacgctt agtaccgctg ttgagatttc gaatacggga ctgtcaccca ctatggtcaa
     3241 gcttcccagc ttgttcttct atctcgacag ttattacgta caggctcctc cgcgttcgct
     3301 cgccactact tgcggaatct cggttgattt cttttcctcc gggtacttag atggttcagt
     3361 tctccgggtt cgcttctcta agtctatgta ttcaacttag gatactgcac agaatgcagt
     3421 gggtttcccc attcggacat cgcgggatca ttgctttatt gccagctccc ccgcgctttt
     3481 cgcaggctta cacgtccttc gtcgcctatc atcgccaagg catccacctg atgcacttat
     3541 tcacttgact ctatcatttc aagaacttct ttgactttgc ctaacattcc gttgactaga
     3601 acatcagact tgaatttcct actttgataa agcttactgc tttgttgtgt cttaatcctg
     3661 ccttttgtgt ttcaggatta agtcgataca atcatcaccc aaatactgtg tttgttttct
     3721 tttctctcgc gagagatttt tatcctttgc aaagaataaa aaatcaaaac aaacgctttg
     3781 tctttgtttg ttgatttcgg ctttccaatt tgttaaagat cgatgcgttc gatattgcta
     3841 tctactgtgc aaatcaaaac gagctgatta ttatatcagc attttgttct tggtcaagtg
     3901 tgacgtcgcc ctgaatggat tctgttccat tcttccgttt tgatttgtac agtattggtg
     3961 gaggcaaacg ggatcgaacc gatgaccccc tgcttgcaaa gcaggtgctc taccaactga
     4021 gctatgcccc cgttcttggt gggtctggga ggacttgaac ctccgacccc acgcttatca
     4081 agcgtgtgct ctaaccagct gagctacaaa cccggattct cttcttaagc gaatcttgcc
     4141 ttcactcaag cttcttccgc atctttttca gtttaccgat aagtgtgaat gcctaaagcc
     4201 tcttctttct ctagaaagga ggtgatccag ccgcaggttc ccctacggct accttgttac
     4261 gacttcaccc cagtcatgaa gcataccgtg gtaagcggac cccttgcggt taccctacct
     4321 acttctggta tcccccactc ccatggtgtg acgggcggtg tgtacaagac ccgggaacgt
     4381 attcaccgca gtatgctgac ctgcgattac tagcgattcc gacttcatgc actcgagttg
     4441 cagagtgcaa tccggactac gatcggtttt gtgagattgg ctccgcctcg cggcttggct
     4501 accctctgta ccgaccattg tatgacgtgt gaagccctgg tcataagggc catgaggact
     4561 tgacgtcatc cccaccttcc tccggcttgt caccggcagt ctcattagag tgcccaactg
     4621 aatgatggca actaatgaca agggttgcgc tcgttgcggg acttaaccca acatctcacg
     4681 acacgagctg acgacagcca tgcagcacct gtgttacggc tcccgaaggc actcctccgt
     4741 ctctggagga ttccgtacat gtcaagacca ggtaaggttc ttcgcgttgc atcgaattaa
     4801 tccacatcat ccaccgcttg tgcgggtccc cgtcaattcc tttgagtttt aatcttgcga
     4861 ccgtactccc caggcggtca atttcacgcg ttagctacgc tactaagcaa tcaagttgcc
     4921 caacagctaa ttgacatcgt ttagggcgtg gactaccagg gtatctaatc ctgtttgcta
     4981 cccacgcttt cgggcatgaa cgtcagtgtt gtcccaggag gctgccttcg ccatcggtat
     5041 tcctccacat ctctacgcat ttcactgcta cacgtggaat tctacctccc tctgacacac
     5101 tcgagtcacc cagttcagaa cgcagttccc gggttgagcc cggggatttc acatcctgct
     5161 taagtaaccg tctgcgcccg ctttacgccc agtaattccg attaacgctc gcaccctacg
     5221 tattaccgcg gctgctggca cgtagttagc cggtgcttat tcttcaggta ccgtcatcag
     5281 ccgctggtat tagcaacagc cttttcttcc ctgacaaaag tcctttacaa cccgaaggcc
     5341 ttcttcagac acgcggcatg gctggatcag gcttgcgccc attgtccaaa attccccact
     5401 gctgcctccc gtaggagtct gggccgtgtc tcagtcccag tgtggcggat catcctctca
     5461 gacccgctac tgatcgtcgc cttggtaggc ctttacccca ccaactagct aatcagatat
     5521 cggccgctcg aatagcgcaa ggcccaaagg tcccctgctt tctctctcaa gacgtatgcg
     5581 gtattagctg atctttcgat cagttatccc ccactactcg gtacgttccg atatgttact
     5641 cacccgttcg ccactcgcca cccgagaagc aagcttctct gtgctgccgt ccgacttgca
     5701 tgtgtaaagc atgccgccag cgttcaatct gagccaggat caaactctta tgttcaatct
     5761 ctaacttttt aacttctggt ctgcttcaaa gaaaccaaca ggacaatgtt caaaacatta
     5821 tcttgtctgt ctttcaaaca gtgtgaggct caaggcactc acacttatcg gtaatctgtt
     5881 ttgttaaaga gcgttgcgaa ttataaagta tctcttccgc ctgtcaagat atctctcgat
     5941 atccccaaca ttctgtgcta tacttttcag ttcgtccgcc gcttctgcag ca
//
LOCUS       DAWHXS010000113         5962 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWHXS010000113 DAWHXS010000000
VERSION     DAWHXS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.113"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(141..728)
                     /locus_tag="WIE22_001761"
     CDS             complement(141..728)
                     /locus_tag="WIE22_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ4127802.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            891..2297
                     /gene="dnaB"
                     /locus_tag="WIE22_001762"
     CDS             891..2297
                     /gene="dnaB"
                     /locus_tag="WIE22_001762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ4127803.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2606..3271
                     /locus_tag="WIE22_001763"
     CDS             2606..3271
                     /locus_tag="WIE22_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ4127804.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCDFGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADSVVWTFNQNGTFGYTN
                     DQHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPKNDTRAV
                     CRH"
     gene            3303..3926
                     /gene="pilV"
                     /locus_tag="WIE22_001764"
     CDS             3303..3926
                     /gene="pilV"
                     /locus_tag="WIE22_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ4127805.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNNTLSHTLSAVDGEFA
                     IDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSLGAAPTLSAGSTFSQNCD
                     GSANGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            3923..4861
                     /locus_tag="WIE22_001765"
     CDS             3923..4861
                     /locus_tag="WIE22_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ4127806.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHPATDVISDTTQQNSP
                     FFLKSKGIDKLIPVAESLNINYQNFFQVDSALIFQYGIDDVNASTATTVVSSCAKIAK
                     PGKKISTLQEAKSVLQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLDDKGK
                     WGNPQLLAKKIRHMKVRYIYVSDCPEDDDAGKEETFKYTDKFNSAKDAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            4840..5433
                     /locus_tag="WIE22_001766"
     CDS             4840..5433
                     /locus_tag="WIE22_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ4127807.1"
                     /translation="MRKQNALTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTANSTNLCIDNKGMEYKKGTRSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5438..5911
                     /locus_tag="WIE22_001767"
     CDS             5438..5911
                     /locus_tag="WIE22_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ4127808.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKNKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWVNSVGDGYKCRDAASARAYSETLSADAGCEAFSNRKK"
BASE COUNT         1629 a         1326 c         1526 g         1481 t
ORIGIN      
        1 tgacagtaac cgtagcaact gaaccgtcat tcccacggaa gtgggaatct agaaataaaa
       61 agcagcaggc atttatcgga aataactgaa attcaatacc gcaaaaatct acccgaaatg
      121 atatagcgga ttaacaaaaa tcaggacaag gcggcaaaac gtttggcgac ttcgtcccag
      181 ttgacgattt cccagaaacc tttcaggtag ttaggacggc tgttgcggta gtcgatgtag
      241 taggcgtgtt cccacacgtc gcaggtcagc agcggcgtgt tttcagtggt cagcggcgta
      301 gcggcgttgg aagtggaaac caaatccaat ccgccggcag gggtttttac cagccacgcc
      361 caaccggagc cgaaagtacc ggccgcgcag gcattgaacg cttcttggaa tttctcgaag
      421 ctgccccatt tcgcgtcgat ggcggcggcc agttcgccgg aaggtttgcc ttgacctttg
      481 ggcgtgaaac ccagccagta gaaagtgtgg ttccaagttt gggctgcgtt gttgaacacg
      541 ccgcccgaag attttttcac aatctcttcc aaaggcaggt tttcaaattc ggtgcctttg
      601 atttgattgt tcaggttggt gatgtaggtt tgatggtgtt tgccgtagtg gaattccaaa
      661 gtttctttgc tcagatgcgg ggacaatgcg tccagttcat aaggcagttg cggcagctta
      721 tgttccattt tgtgctccta atattgtttt aaatgttgta ttttggcagt gttgctgcaa
      781 atagctcagc agcgcacata ttttacctgt tttgagggat ggaaaccaat taaacctgct
      841 ttacgctata atagaagatt gcaatttcgg cacgacagat aggatgtacc atgaacgatt
      901 acacagctat gccgtctgaa gaccgtgagg tcggcgcatt gtcgctgcct ccacattcaa
      961 tggaggcaga acaatccgtt ttgggcggtt tgatgctgga aaatccggca tgggacagga
     1021 ttgccgatgt ggtttccggt gaagacttct accgccacga acaccgcctg attttccgat
     1081 ccattgctaa attaatcaat gagagccgtc ccgccgatgt gattacggtt caggaagatt
     1141 tgcagcggaa cgaagaattg gaagcggcag gcggattcga atatctgatt acgctggcgc
     1201 aaaacacccc gtctgccgcc aacatccgcc gctacgccga aatcgtgcgc gagcgttcca
     1261 tcatgcgcca actcgccgaa gtggggacgg aaatcgcccg cagcgcatac aatccgcaag
     1321 gcagggacgc ggggcagctt ttggacgagg cggaaaacaa agtattccaa atcgccgaaa
     1381 gcaccgccaa atccaagcag ggctttttgg agatgcccga tttgctgaaa gaagtcgtac
     1441 agcgcatcga tatgctctac tcgcgcgaca atcccgatga agttaccggc gtgccgacgg
     1501 ggttcatcga cctcgacaaa aaaacctcgg gccttcagcc cggcgacctg attattgttg
     1561 ccggtcgtcc gtctatgggt aagaccgcct tttctatcaa tatcgccgaa cacgttgccg
     1621 tagaaggcaa gctgccggtt gccgtcttct caatggagat gggcggtgcg caactggtca
     1681 tgcgtatgct cggctcggtc ggacgcttgg atcaaagcgt cctgaaaacc ggcaggctcg
     1741 aagacgaaca ctggggtcgt ctgaacgaag cagtcgtcaa actctccgac gcgcccgtgt
     1801 acatcgacga gaccccgggt ctgactgcgc tcgaactgcg cgcccgcgcc cgtcgtctcg
     1861 cccgtcaatt taacaataag ctgggattaa tcgtcatcga ctacctgcaa ctgatggcgg
     1921 gatccggccg ttccgacaac cgtgcttcag agctgggcga aatttcacgt tcgctcaaag
     1981 cgttggcgaa agaattgcaa gtccccatca tcgccctgtc gcaattgagc cgtaccgtcg
     2041 aatcgcgtac cgacaaacgc ccgatgatgt ccgacctccg cgaatccggt gctatcgagc
     2101 aagatgccga cctgattatg ttcatgtacc gcgacgaata ctacaaccag gactcaccca
     2161 tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa cggtcccgtc ggtaaaatct
     2221 tcctcacatg gacgggacaa ttcaccaaat tcgacaatgc tgcctatatt cccgaggagg
     2281 caaagataga ggattaaatg gctatataaa aatttattag gcgaaatcag gcaaaatcgt
     2341 ttaaaatcat gctgagagat tgccctaaaa aataaaacgc ggtcttgagg catttttgca
     2401 ttcagcccgc atataattga aaatatagtg gattaacaaa aatcaggaca aggcaacgaa
     2461 gccgcagacc gtacaaatag tacggaaccg attcacttgg tgcttgagca ccttagagaa
     2521 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttgaatttaa tccactatac
     2581 attaccagtt aacgttctat tgcttatgtg tacacgaaaa caacaaggtt tcacgctaac
     2641 agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc tccccaatat
     2701 gagccaatgg attgcatccc gccgcattgc cagtcacgcg gagcaggttg ccaacctttt
     2761 gcgtttctcc aggggagaag ccgtccggct caatcttcct gtctatatct gtcctgttca
     2821 agttaaaaaa ggcggtgcgc ccaacaataa atgtgacttc ggcaagaagg ggcagggaat
     2881 gttggctttt ggcgataaga atggcaataa gacatatgac ggcgatacgg cggatgttct
     2941 ccttcgcagt gtggtattga atgatgatat caatgataag cggattaatt atgccttcaa
     3001 ccatatcgct ttcggtcaaa cgcagccgac cgccgacagc gttgtttgga cgttcaatca
     3061 aaatgggacg tttggttata cgaatgacca gcatcttaca aaacaatcca gcttttttta
     3121 ttccgacggt tatatccaaa tcgtgctgac agatgcgaga gcagtttcag atgccgataa
     3181 gaaattccgt tcggcggtgg ttttgattaa cagcagcggc agggttgaag tttgtcctaa
     3241 aaacgatacg cgcgccgtat gccgacatta atgattgttt tagtcggttt gggaaataaa
     3301 aaatgaagaa taatgattgc ttgcgcctga aaaatcccca gtccggtatg gcgctgatag
     3361 aagtcttggt tgctatgctc gttctgacca tcggtatttt ggcgttactg tctgtgcagt
     3421 tgcggacagt cgcttccgtc agggaggcag agacgcaaac catcgtcagt caaatcacgc
     3481 aaaacctgat ggaaggaatg ttgatgaatc cgaccattga ttcggacagc aacaagaaaa
     3541 actataatct ttacatggga aacaatacac tatcgcatac actatcagct gtggatggtg
     3601 agtttgcgat tgatgccata aaaactaaga cgcagttggc agaggcacaa ttgaagagat
     3661 ttagttatga gctgaaaaat gccttgccgg atgcggcagc catccattac gccgtctgca
     3721 aggattcgtt gggtgctgcg ccgacattgt ccgccggcag tactttttct caaaattgcg
     3781 atggtagtgc aaatggggat actttgatta aagtattgtg ggtaaatgat tcggcagggg
     3841 attcggatat cgcccgtacg aatcttgaga cgaacggcaa caatatcgta tatacctatc
     3901 aggcaagggt cggaggtcgg gaatgagacg taaaatgcta aacgtaccaa aaggcagtta
     3961 tgatggtatg aagggtttta ccattattga atttttggtt gcgggcatgc tcagtatgat
     4021 tgtcctgatg gcggtcggat cgagttactt cacgtcccgg aaattaaatg atgcggcaaa
     4081 cgagcgtctt tccgcacaac aggatttgcg gaatgcggca acattgattg ttcgcgatgc
     4141 aagaatggca gggggcttcg gttgtttcaa tatgtccgag catcctgcaa ctgatgttat
     4201 ttccgatacg acgcaacaaa attctccttt tttcttaaaa agcaaaggca tagataaact
     4261 tatccccgtg gcggaatctt taaatatcaa ttatcagaat tttttccagg ttgatagcgc
     4321 attgattttt caatacggaa tcgatgatgt taatgcaagc accgcgacta ccgtcgtcag
     4381 cagctgtgcc aaaatagcaa aaccgggtaa gaaaatatct accttgcaag aagcaaagag
     4441 tgtattacag attactaatg atgataaaca aaatggaaat atcacccgtc aaaggcatgt
     4501 ggtcaatgcc tatgcggtcg gcaggattgc cggtgaggaa ggtttgttcc gcttccaatt
     4561 ggatgataag ggcaagtggg gtaatcctca gttgctcgcg aaaaagatta gacatatgaa
     4621 agtgcggtat atctatgttt ccgactgtcc tgaagatgac gatgccggca aagaggaaac
     4681 attcaaatat acggataaat tcaacagcgc caaagatgct gttacgcccg ccggggtgga
     4741 ggttttattg agtagcggta ctgataccaa gattgccgct tcttcagaca atcatattta
     4801 tgcttaccgt atcgatgcga caatacgcgg gggaaatgta tgcgcaaaca gaacgctttg
     4861 acgggaatcc cgacttctga cggacagagg gggttcgcgc tgtttatcgt actgatggtg
     4921 atgatcgtcg tggctttttt ggttgtaact gccgcgcagt cttacaatac cgagcagcgg
     4981 atcagtgcca acgaatcaga caggaaattg gctttgtctt tggccgaggc ggctttgcgg
     5041 gaaggcgaac ttcaggtttt ggatttggaa tatgatacgg acagtaaggt tacatttagc
     5101 gaaaactgtg gaaaaggtct gtgtaccgca gtgaatgtgc ggacaaataa tgataatgaa
     5161 gaggcttttg acaatatcgt ggtgcaaggc aagcccaccg ttgaggcggt gaagcgttct
     5221 tgcactgcaa attctaccaa cctgtgcatt gacaataaag ggatggaata taagaaaggc
     5281 acgagaagcg tcagcaaacc accgcgctat attatcgaat atttgggcgt gaagaacgga
     5341 gaaaatgttt atcgggttac tgccaaggct tggggtaaga atgccaatac cgtggtcgtc
     5401 cttcaatctt atgtaagcaa taatgatgag taataaaatg gaacaaaaag ggtttacatt
     5461 gattgagatg atgatagtcg tcgcgatact cggcatcatc agcgtcattg ccataccttc
     5521 ttatcaaagt tatattgaaa aaggctatca gtcccagctt tatacggaga tggtcggtat
     5581 caacaatatt tccaaacagt ttattttgaa aaatcccctg gacgataatc agaccatcaa
     5641 gaacaaactg gaaatatttg tctcaggcta taagatgaat ccgaaaattg ccgaaaaata
     5701 taatgtttcg gtgcattttg tcaataagga aaaaccaagg gcatacagct tggtcggtgt
     5761 tccaaagacg gggacgggtt ataccttgtc ggtatgggtg aacagcgtgg gcgacggata
     5821 caaatgccgt gatgccgctt ctgcccgagc ctattcggag actttgtccg cggatgccgg
     5881 ctgtgaagcc ttctctaatc gtaaaaaata gggctgtttt gccgatgccg tctgaaaatc
     5941 aatgttcaga cggcattttt at
//
LOCUS       DAWHXS010000114         5948 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWHXS010000114 DAWHXS010000000
VERSION     DAWHXS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.114"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            141..722
                     /locus_tag="WIE22_001768"
     CDS             141..722
                     /locus_tag="WIE22_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ4127809.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(784..1638)
                     /gene="folD"
                     /locus_tag="WIE22_001769"
     CDS             complement(784..1638)
                     /gene="folD"
                     /locus_tag="WIE22_001769"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ4127810.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHTPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1861..1938
                     /locus_tag="WIE22_001770"
     tRNA            1861..1938
                     /locus_tag="WIE22_001770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1896..1898,aa:Pro,seq:tgg)
     gene            1963..2039
                     /locus_tag="WIE22_001771"
     tRNA            1963..2039
                     /locus_tag="WIE22_001771"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1997..1999,aa:Arg,seq:tct)
     gene            2067..2142
                     /locus_tag="WIE22_001772"
     tRNA            2067..2142
                     /locus_tag="WIE22_001772"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2100..2102,aa:His,seq:gtg)
     gene            2354..2860
                     /gene="rfaE2"
                     /locus_tag="WIE22_001773"
     CDS             2354..2860
                     /gene="rfaE2"
                     /locus_tag="WIE22_001773"
                     /inference="COORDINATES: protein motif:HMM:NF013621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEZ4127811.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGDALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2857..4635
                     /locus_tag="WIE22_001774"
     CDS             2857..4635
                     /locus_tag="WIE22_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ4127812.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4748..5572
                     /locus_tag="WIE22_001775"
     CDS             4748..5572
                     /locus_tag="WIE22_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ4127813.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAQAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADAEEIRKITSLYGKLNLKSCK"
BASE COUNT         1302 a         1514 c         1780 g         1352 t
ORIGIN      
        1 tatgtatggc gtaaaggaag cgttttaacg ccgtattggg aaaatgtaaa gttctttatc
       61 gggatttctt taagtattgg cgttttcggt gtcgtggcgg tagaatcggg gtcagttaag
      121 aacaattatc aggaacggat atggaagaaa aaaacgacta caccgacgcg gcatccgaca
      181 accgcaacgg gcaggaaatt gaagtcggaa ttgccggcgc aggcgaccgg atacttgccg
      241 cgctgctgaa ccaattgttt acctttttgg ttttgttggt gccgtttgcc ggactgattg
      301 ctttcgccat caagaatgaa ggcaggattg gcggcagtga ggaaatattc gggctgcttt
      361 tgggcatgac ttccttttgg gtaggactgg cgggcatttt ggtttatacc gtcgttcaaa
      421 tctactatat gagcagggac gggcagtcat tgggtaagaa gatcatgaga atcagggtgt
      481 tgaaaaccga cggccgcaat ccgggttttg tcggcacggt tttggtacgc gaaatcgcat
      541 ggtcggtttt ggttgccatt attgccgccg ttatcggtct tgcagtaggt gacaacggag
      601 aaaacgccat caacctgctg gcattccttg ccaactttgt cctgctcttt atggtcaaac
      661 gcgaccgccg cacgctttac gacatactgg cggatacggt tgtcgtcaag ctgcccaaat
      721 aaacggacac ggcaaaatgc cgtctgaaag cctttcagac ggcattttta tcttcagaac
      781 cgcttaagca tcgtgcagcg aagccgcgtg caaagtgttt tccattaatg tggcaatcgt
      841 catcggaccc acgccgccgg gaacgggcgt aatcatcgcc gcccgttctt ttgccgtttc
      901 aaattccacg tcgccgcaca ggctgccatc gtccaaacgg ttgatgccca catcaataac
      961 gaccgcgcca ggtttgatcc attcgccttt gacaaagttc ggaatgccta cgccgaccac
     1021 caaaatatcg gctccggcaa cctcgtctgt cagattttcg gttgcgctgt ggcagaccgt
     1081 taccgttgcg cgcgccagca gcagttccaa agcctgcggg cggccgacga tattcgacgc
     1141 gccgaccacg accgcttttt tccccttcgg atcaatgccg taagcttcca aaagcgtcat
     1201 cacgcccttg ggcgtacacg ggcgcatcag cggcattttg accgccagcc tgccgacatt
     1261 gtaaggatgg aagccgtcca cgtccttatc cggcgaaata cgttccaaaa ccgcctggct
     1321 gtcgaggtgc ttcggcagcg gtagctgaac cagaataccg tccacttcgg aatcggcatt
     1381 caggcggtcg accagtgcca gcagttcttc ctgcgatgtt gattcgggca gctcgtaaga
     1441 cagtgatttg atgccgcatt tttggcaggc agttttcttg ttgcggacat aaaccgcgct
     1501 ggcagggtcg cctccgacca aaaccacggc caggcaaggc gtgtgcagat tgtcctgttg
     1561 gcgttgcgcc accgcttcgg caaccgcctg caggcgtttt tgcgaaactt ctttaccatt
     1621 gatcagttgt gccgacatat ccgtcctttt gttaaacagg tagaaaatgc tgaaaattat
     1681 cgcattttcc gtttgttttt acagcaggat gccgaaaata aatttgccct gaaataaaat
     1741 acggctgttt ggacggatta aatatttttt caaatcaaaa aactatattt ttcatcttgg
     1801 tgtcttgaaa ataaatattg acgcatcttt ccctgagttg tatagttcgc ttcttcaagt
     1861 cggggcgtag cgcagcccgg ttagcgcatc tgctttggga gcagagggtc gtgagttcga
     1921 atcccaccgc cccgaccaat caatcagcac gaatcggcat gagcgcccgt agctcaaccg
     1981 gatagagcac cgaccttcta agtcgggggt tacaggttcg attcctgtcg ggcgtgccaa
     2041 gaatttgatt gaaaaccgtt tttatggtgg ctgtagctca gttggtagag ccccggattg
     2101 tgattccggt tgtcgtgggt tcgagcccca tcagccaccc cagataaagg cccgtacttt
     2161 tcggtacggg tctttttccg tttgcacgta ctgtccgcac cgtcgtaccg gtgcggcggt
     2221 atgccgtctg aaagccggac cggtcatacg gaaaccgctt cagacggcat ttgcgccgcc
     2281 ggctttgcct ttcgcatatg cttctgtatc atgtgggttt ttctgtcttt tatggttttt
     2341 gtcggaggct ttgatggttg acgcttggtc tgtccctgat tttgaatcga aaatctgtcc
     2401 gcccgaggcg ttggcggcgc gtttggcgtt gttgccgcgc ccgctggtgt ttaccaacgg
     2461 ctgtttcgac atcctccaca gggggcacgt tacttatctg gcgcaggcgc gttcgatggg
     2521 ggacgcgttg gtgctggcgt tgaataccga tgcttcggtg cggcgtctgg gcaagggcgg
     2581 tgaccgcccg gttaatcctt tggagaaccg tgccgccgtt gccgccgcgt tggaaagtgt
     2641 ggatttggta acgtggtttg acggggatac gccggcggcg ttgattgagg cggtcaaacc
     2701 tgagattttg gtcaagggcg gcgattgggc tgcggataag attgtcggtg cggcagaaac
     2761 gttggcgcgc ggcggtcagg tgttttcaat tccgtttctg caccagactt cgacaacgaa
     2821 gactttggca aaaatccgtg cggcagaggg cggaaaatga cggttttgaa gctttcgcac
     2881 tggcgggtgt tggcggagct tgccgacggt ttgccgcaac acgtctcgca actggcgcgt
     2941 atggcggata tgaagccgca gcagctcaac ggtttttggc agcagatgcc ggcgcacata
     3001 cgcgggctgt tgcgccaaca cgacggctat tggcggctgg tgcgcccatt ggcggttttc
     3061 gatgccgaag gtttgcgcga gctgggggaa aggtcgggtt ttcagacggc attgaagcac
     3121 gagtgcgcgt ccagcaacga cgagatactg gaattggcgc ggattgcgcc ggacaaggcg
     3181 cacaaaacca tatgtgtgac ccacctgcaa agtaagggca gggggcggca ggggcggaag
     3241 tggtcgcacc gtttgggcga gtgtctgatg ttcagttttg gctgggtgtt tgaccggccg
     3301 caatatgagc tgggttcgct gtcgcctgtt gcggcagtgg cgtgtcggcg cgccttgtcg
     3361 cgtttaggtt tggatgtgca gattaagtgg cccaatgatt tggttgtcgg acgcgacaaa
     3421 ttgggcggca ttctgattga aacggtcagg acgggcggca aaacggttgc cgtggtcggt
     3481 atcggcatca atttcgtgct gcccaaggaa gtagaaaatg ccgcttccgt gcaatcgctg
     3541 tttcagacgg catcgcggcg gggaaatgcc gatgccgccg tgttgctgga aacgctgttg
     3601 gcggaacttg atgcggtgtt gttgcaatat gcgcgggacg gatttgcgcc ttttgtggcg
     3661 gaatatcagg ctgccaaccg cgaccacggc aaggcggtat tgctgttgcg cgacggcgaa
     3721 accgtgttcg aaggcacggt taaaggcgtg gacggacaag gcgttctgca cttggaaacg
     3781 gcagagggcg aacagacggt cgtcagcggc gaaatcagcc tgcggcccga cgacaggccg
     3841 gtttccgtgc cgaagcggcg ggattcggaa cgttttctgc tgttggacgg cggcaacagc
     3901 cggctcaagt gggcgtgggt ggaaaacggc acgttcgcaa ccgtcggtag cgcgccgtac
     3961 cgcgatttgt cgcctttggg cgcggagtgg gcggaaaagg cggatggaaa tgtccgcatc
     4021 gtcggttgcg ctgtgtgcgg agaattcaaa aaggcacaag tgcaggaaca gctcgcccga
     4081 aaaatcgagt ggctgccgtc ttcggcacag gctttgggca tacgcaacca ctaccgccac
     4141 cccgaagaac acggttccga ccgctggttc aacgccttgg gcagccgccg cttcagccgc
     4201 aacgcctgcg tcgtcgtcag ttgcggcacg gcggtaacgg ttgacgcgct caccgatgac
     4261 ggacattatc tcgggggaac catcatgccc ggtttccacc tgatgaaaga atcgctcgcc
     4321 gtccgaaccg ccaacctcaa ccggcacgcc ggtaagcgtt atcctttccc gaccacaacg
     4381 ggcaatgccg tcgccagcgg catgatggat gcggtttgcg gctcggttat gatgatgcac
     4441 gggcgtttga aagaaaaaac cggggcgggc aagcctgtcg atgtcatcat taccggcggc
     4501 ggcgcggcaa aagttgccga agccctgccg cctgcatttt tggcggaaaa taccgtgcgc
     4561 gtggcggaca acctcgtcat tcacgggctg ctgaacctga ttgccgccga aggcggggaa
     4621 tcggaacata cttaaaaaat acaccctttt tcaacacggg gcggtatccg tccgaatccg
     4681 tccggattct gccgcccgac agactgccgc ccaggcgggc ggggtttcaa acattgagga
     4741 aattctaatg aaatggctct tcaccctttt ggcggtactc aatattgccg tattcggcgg
     4801 cacggtgggc tataaattgg ccgttaaagc gacgggcggc gtgccggaaa accgggccgt
     4861 cgaaaacacg ccccccgcaa cgcccgccgc cgacaatgcg gcggcatccg tggaggatac
     4921 ggcggcactg ctcaaaccgg gcgacatcct gaccgaagag caggcggagc agttgcgctt
     4981 gaagaaagaa gcggaacaga aaaaactgag ggagaaaaaa cagcgtgaag aaaaagcccg
     5041 ccgcgaaaaa ctcgccgccg aaaaggcgca ggcggaacgc gaaaacggcg cggcggatgc
     5101 cttatgcgcc gcgcaggcaa gcctcacgat ggacgaagat gactaccacc gcatcaaagg
     5161 acttttgggc aaatggtcgc acgttgccag caggagcgtc gaaaaacgca ccgcccaagc
     5221 caaacctgcc gacaaaacct accgcgtcgt cctgcccgtt tccgccgatg ccgaaaatca
     5281 ggcggcggag ctgtctgcca aaggtttcaa ccccataccg tttgacggcg cattgagttt
     5341 gggtgtcggc aacagccggg aaaacgccca agccctgcaa aaccggcttg ccggcgccgg
     5401 attcggcggg gcgcatattg tcgaacactt tgccgaagcc gacaggcagg acgattcttt
     5461 gagcgtgtcg cgtatgacgg ttttgtttac cggcgtgaat gccgccgatg cggaagaaat
     5521 tcgtaaaatc acgtccctat acggcaaact gaacctcaag tcttgcaaat aggcgcgaaa
     5581 gccggacgat aaacgccaaa cccaccgcga aggtgggttt ggcgtttcgg ttttgagtat
     5641 ctgatatata aaaagggaat gtatgtctcg tatatgccga ctccaagtgt gaaagtgatg
     5701 atggggaaat atggtgttta atggttcgga acggacggct tccgagtggt tttgattata
     5761 cgctaataat aataattatc aataaaaatt taaaaataaa tgcagatttt ttgtaatacg
     5821 atgaaaaata aacaaattaa cctgtccgcc gatgtttgtg attcgggtcg gcaaactttc
     5881 attgtgtcgg acaaatcggg gtcggcagtc atagctgagg gcttcgtgcc tgacggaaat
     5941 aaggttgc
//
LOCUS       DAWHXS010000115         5859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWHXS010000115 DAWHXS010000000
VERSION     DAWHXS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.115"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1016..1786)
                     /gene="xth"
                     /locus_tag="WIE22_001776"
     CDS             complement(1016..1786)
                     /gene="xth"
                     /locus_tag="WIE22_001776"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ4127814.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1846..2127)
                     /locus_tag="WIE22_001777"
     CDS             complement(1846..2127)
                     /locus_tag="WIE22_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ4127815.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(2533..3414)
                     /gene="nadC"
                     /locus_tag="WIE22_001778"
     CDS             complement(2533..3414)
                     /gene="nadC"
                     /locus_tag="WIE22_001778"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ4127816.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(3490..4428)
                     /locus_tag="WIE22_001779"
     CDS             complement(3490..4428)
                     /locus_tag="WIE22_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127817.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            4735..5847
                     /gene="nadA"
                     /locus_tag="WIE22_001780"
     CDS             4735..5847
                     /gene="nadA"
                     /locus_tag="WIE22_001780"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ4127818.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSHPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
BASE COUNT         1360 a         1486 c         1589 g         1424 t
ORIGIN      
        1 ctgatgcaga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
       61 caaatagtac ggcaacgctg tactggttta aatttaatcc actatatttt gtaatagctg
      121 ccattatgcc tactaggata tataccttct atataatcct tatttaaata aaaaccctta
      181 taaaaatctg tgttatctgt cgggattgaa aaaaagattt tatctttctc atcaggttta
      241 tatttgctat caatatcaaa aagagaataa ggattacaat gagttcgaca tactaggtaa
      301 aaatttatat cttggatcat gaaatattcg atatttatca ggatcgaaag atgctaaaaa
      361 ttcatcaata ttggtactgg atattttgtc atcactaata aacatcgcta gagatgggcg
      421 actttgattt tccatttgtt gattagtaaa ggatataaat actaacttgc cattgtcaaa
      481 ccacatagat ttatatagtg gattaaattt aaaccagtac gtcgttgcct cgccttgccg
      541 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatag
      601 ttattgcctt tgggagaatc aattctgaca gcacataaca taggttcatc tttacagtcg
      661 ttatattggg tttcatcaaa ctcaactgca tagcacaata aaggcaatag gaaaaaagga
      721 gattaattta ttactataca taaggcagcc tgactattca ttctaaaata aatccggttt
      781 ttaatatttg attctgctcc ggattatata cataataaat tttactgctc caaaatctga
      841 tattaaccct atggatttca taaatttttc agaaaaatag gctgtgcttg aacaatccgc
      901 cgactccggc tgtttgtcag ggaaaaggca attattttcg gttagtcagc agcctatcct
      961 attgaatcac ttaaagatat ggatgtttgt caacgccata tcaaaacacg gtcttttacc
     1021 aatcgaattc tgccgtcacc ggcgcgtggt cgctcggacg ctccagcgcg cgcgtctcca
     1081 aatcgacgcg gacatccttc aacgccgccg ccatcgcagg cgacaccaaa atatggtcga
     1141 tacgcaggcc cagtttgcgt tggaacatcg cgccgcgata gtcgaaccag gtatagaacg
     1201 cgccttcggg atggacttgg cgcaggctgt cggtcagtcc caaatccagc aggttttgaa
     1261 accactgccg ttcgacggac gaacagtgga ttttttcgtg ccatttttca gggtcgtaac
     1321 agtccgcatc ggcaggcgcg atattgaaat cgcccagcaa caccagtttg ccgtggcggg
     1381 tcatttcatc gcggacaaac tccgtcagtg cggcaaacca ctgttcctta tatttgaatt
     1441 tggggctgtc caaagcctcg ccgttgacgc aatagacatt gatgacgcgc acgccgctga
     1501 cggttgccgc aatcacgcgc cgttgcggat cgtccggcag tgcgggcaaa ccgaaatgca
     1561 cgtcctgcgg cacgctgcgg ctgacgattg ccacgccgtt gtaggttttc tgcccgctcc
     1621 aaacacagtg ccagcccatc atttgcaaag cggcggccgg aaatttgtcc tgatcgagtt
     1681 tgagttcctg caaaaccaaa atatcgggcg gattgtcggc aagcaggttt tgcacctgcg
     1741 gcagccgcac attgagcgaa ttgacattcc aagtggtgat tttcataaca tttccgaaaa
     1801 atgctgtctg aaacgttcag acggcaaaag gaaaggattc taacactatg ccacgcgttt
     1861 gttttccaaa tcgcgaaccg tgtgcagaat cgccgccgct tcttcggaaa ccaggcggta
     1921 ttcgataatg cggtggtaac gcgtaaaatc gactagacct tccacgcgca ggcggttcaa
     1981 atggttggaa actgcggtgg ccggcaggga taaggatttt gccagttcgg cgatattgcg
     2041 ttcgctgtcc aacaattgaa tcagtatcgc catacgttcg ggatgcgcca taagtttgag
     2101 tatggtgtgg agcggtattg tgttcatcgg tattcctaaa acctttgccg cccgccaagc
     2161 ggattgacgg gcggcagata tggatgattg tgattatctt tataaatcgt attataaatc
     2221 aaatatttat ggatgattgt acaggattgt atgttccttt tcccactttt ttgcaaacaa
     2281 gcgtaaaggc gggctttcag acggcataaa actgccgccc cccgtccttt ttcctgccct
     2341 accgatgccg ccgcgctgtc gggcaaacaa aatacctgcc tgaaatgctg ttttttctaa
     2401 agcatcacga cctttacgaa aaatgccgtc tgaagaatct ttcagacggc atttcctccc
     2461 tgaggcaata accggattag ggttaagcgg ttccttgcct gacatatcag acagccgccc
     2521 gcaccctaaa actcacgcca cgaaatccaa acctatgtcc aacgaacggc tgctgtgggt
     2581 cagatagccg agggcgatgc cgtccactcc ggtttgcgcc acgcgcttca ggcggtcgaa
     2641 gccgatgccg cccgatgctt cgcaatagat ggtgtggggg tgggcggttt gcgtgtggca
     2701 gcggtttgcc gcttctttca gggtttcgtc gtccatgtta tccagcaaaa tccgttccgc
     2761 gcccgctgcg atggcttcgt ccagttgtgc caacgtatcc acttcgattt ccacgcaggt
     2821 caatgctccg acagcctgtt ttgcctgctg cacggcttgg gcgatgctgc cgcaataggc
     2881 gaggtggttg tctttgatga gcacggcgtc gtccaaaccc atgcggtggt tcacaccgcc
     2941 gcctgccctg acggcgtatt tttgcaggac acgcagcagg gggatggttt tgcggctgca
     3001 cacgatgtct gtaccgtatt cggcgacttc ggcaacggca cgcgcggtgg cggtggcgat
     3061 gccgcttaag tgcgtgaggt agttgagcgc ggtgcgttcg gcggcgagca gcgcgcgggc
     3121 gttgccttcg acggcggcaa gcgtctgacc tgcgcggacg gcttgcccgt ctcggatttc
     3181 ggcttggaag cggacggacg gatccatcgt ctgaaaggcg aggcgcgcta aatccatgcc
     3241 ggcgataacg ccgtcttcgc ggctgacgag gaagagtttg gcggttttgt cgggggcgat
     3301 aacggcggcg gacgtaatgt cgccgcgcct gcccaagtct tcgctcaagg cttgttcgac
     3361 tatggggcgc aacagggtgt cgggcaggga aaagagggtg ttttcagacg gcatgaggtt
     3421 tcctttgtcg aaaaagattg atgttgcacc gatggggcaa aacttcattt gatgccgtct
     3481 gaaaacgggc taacggctgc ccagcggcaa tccgatgtcc gaaatcagcc tgtcgggcag
     3541 gacgtcgtcg cggaagcggc aaagctgcgc gggacggcct ttgctgcccg atacgccggt
     3601 atccgacggc tcgataaggt tttgctgctg aatctggcgg cggaagtttt gcttgtgcag
     3661 caatcttccg ctgatggctt cgacactgtt ttgcagttgc agcagcgtga attcgggcgg
     3721 catcagttca aaaatcacgg ggcggtattt gattttggcg cgcaggcggg acagggcggt
     3781 cgccagtacg cgacggtgat cgtggcgcat gggctgcccc gtgagcgcga agtcgaagtt
     3841 tgcctgcggc tcggcggctt ccactatcag accgctttca tacagcattt catagcgttg
     3901 taaaacgtat tcttccgacc agttttccgg ttcgaccccc cagcacaaat gaatgcgctt
     3961 gagccgcatt tgacgcactt cctccgtgtc cgccgaattt gcccaaatgc gcaggcggct
     4021 gacgacggcg tcgcgctgcc cgccgtcggt gcgcaagtct tcccacggga aatagccgta
     4081 gcagtcctgc catttcgcat ccgggtgcag gatgctgtcg gctgcctcgc gcaccagccc
     4141 caaatagctg acgtacaaga cgggcatgcc gtgttcgttg cggcggtggg tatcgacaaa
     4201 ggtgtaaagc tgttccacat agcccatagg ctgcgaagtc tgcttggcaa cccacagctt
     4261 cacgcccgcc tgcagggaat tgcgcagagg ggagagcggg ccgttgggca ggagtgtgcc
     4321 ttgggcgacg gttaataccc gcaggccgcc gtcggtaacg gcaatcaata cgggaaccag
     4381 ctcgacgatg ctttgcggcg gggcttcggc ttcggggtag gcatccatgc tcggctttct
     4441 gcgggtggga aaggctttat tgtatagtgg attaaattta aaccagtaca gcgttgcctc
     4501 gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
     4561 ccactataaa ccaaagcggt ttgtcggaac gaagcgggtc ggttttacgg tatcgcaaac
     4621 ctgttttcag acgaccttgc ttattatact caatttgagc ataatataaa aatatttcaa
     4681 aatataaacg gcaaatcttt atgccgtctg aaaaaccagc cataaggaag tctcatgcaa
     4741 accgccgccc gccgctcgtt cgattacgat atgcccctca tccaaacccc gacttccgcc
     4801 tgccaaatcc gtcaggcgtg ggcgaaggtt gccgacacgc ccgaccgcga gacggcaggc
     4861 cgtctgaaag acgaaatcaa ggctttgctg aaggagaaaa acgcggtctt ggtggcgcat
     4921 tattacgtcg atccgctgat tcaggatttg gctttagaaa cgggcggatg cgtgggcgat
     4981 tcgctggaaa tggcgcgctt cggcgcggaa cacgaagcta gtacattggt ggtggcgggt
     5041 gtgcgcttca tgggcgagag cgcgaaaatc ctctgccctg aaaaaacagt gctgatgcct
     5101 gatttggagg cggaatgttc tttggatttg ggctgtccgg aagaagcgtt ttcagcgttt
     5161 tgcgaccaac accccgaccg cacggtcgta gtgtacgcca acacttccgc cgccgtgaaa
     5221 gcgcgtgccg attgggtggt aacgtcttcg gtggcgttgg aaatcgtatc gtatttgaaa
     5281 tcgcgcggcg agaagctgat ttggggacct gaccgccacc tcggcgacta catccgccgc
     5341 gaaacgggcg cggatatgct gctgtggcag ggttcgtgca tcgtccacaa cgaattcaaa
     5401 gggcaggagc tggcggcgtt gaaggcggaa caccccgaag cggtggtgct ggttcatccc
     5461 gaatcgccac aaagcgtcat cgaactgggc gacgtggtcg gctcgaccag caaactgctc
     5521 aaagccgccg tatcgcaccc tgaaaaaaaa ttcatcgtag cgaccgactt gggcatcctg
     5581 cacgaaatgc aaaagcaggc acccgacaaa gaatttatcg ccgcaccgac ggcgggcaac
     5641 ggtggaagct gtaaaagctg cgcgttctgc ccttggatgg cgatgaattc gctgggcggc
     5701 atcaaatacg ccctgacaag cggacacaac gaaatcctgt tggacaggaa gctgggcgaa
     5761 gccgccaaac tgcctttgca gcgtatgctc gacttcgcgg caggactcaa gcgcggggat
     5821 gtgttcaacg gcatgggacc cgcctgattt gccgtccat
//
LOCUS       DAWHXS010000116         5805 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWHXS010000116 DAWHXS010000000
VERSION     DAWHXS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.116"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(225..380)
                     /gene="rpmG"
                     /locus_tag="WIE22_001781"
     CDS             complement(225..380)
                     /gene="rpmG"
                     /locus_tag="WIE22_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ4127819.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(412..645)
                     /gene="rpmB"
                     /locus_tag="WIE22_001782"
     CDS             complement(412..645)
                     /gene="rpmB"
                     /locus_tag="WIE22_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ4127820.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(895..2421)
                     /gene="farB"
                     /locus_tag="WIE22_001783"
     CDS             complement(895..2421)
                     /gene="farB"
                     /locus_tag="WIE22_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ4127821.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPIIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2445..3617)
                     /gene="farA"
                     /locus_tag="WIE22_001784"
     CDS             complement(2445..3617)
                     /gene="farA"
                     /locus_tag="WIE22_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ4127822.1"
                     /translation="MATHADETTIQNRQEANSAKVKRKRRLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(3661..4020)
                     /locus_tag="WIE22_001785"
     CDS             complement(3661..4020)
                     /locus_tag="WIE22_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ4127823.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      4235..4279
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            4283..4756
                     /gene="queF"
                     /locus_tag="WIE22_001786"
     CDS             4283..4756
                     /gene="queF"
                     /locus_tag="WIE22_001786"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ4127824.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            4833..5519
                     /locus_tag="WIE22_001787"
     CDS             4833..5519
                     /locus_tag="WIE22_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ4127825.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYIFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1460 a         1613 c         1325 g         1407 t
ORIGIN      
        1 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc tgcagacagt acaaatagta
       61 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctatc cttggcagac
      121 ggtttgcagc agattcgcat acggcagatg tttcaagcgg aaaagaaaca cgataaaaat
      181 aaaaaagcct ccgaacagtc ggaggctttg ctttcaaact gaaattattt cagtttggtt
      241 tctttgtaca ctacgtgttt gcgggcaacc ggatcgaatt ttttaatttc cagtttgccg
      301 ggcatagtgc gtttattttt ggtagtggtg tagaagtgac cagtacctgc actggattcc
      361 agtttgattt tatcgcgcat tgcagtaatc cttaattaaa tagtgtttaa attaagcttc
      421 gccgcgagca cgcaaatcag ccaatacgac atcaatgcct actttgtcga tggtacgcag
      481 tgcagcgttg gaaacgcgca ggcgaaccca gcggttttca ctttctaccc aaaaacgacg
      541 tgattgcaag ttgggcaaaa aacggcgttt ggttttgttg ttggcgtgcg atacgttgtt
      601 gccggacatc gggcgtttgc cggtcacttt gcaaactcgt gccataatca gtctccaaac
      661 ttctaaaata gtaaaacgcg atttatacca cgaaaaacag tgttagttca agtgttttaa
      721 agaaaaacgg ctgttttgcg cggataatcc ttccgattgt cgcatctgtg gaacgtttgc
      781 aggtgcgaaa cagatggggc gcgattatag cggatttcgg gaactgctgc attcaaaatg
      841 ccgtccgaac atatttccag tattttcaga cggcatttca agttttcaaa tccctcaatg
      901 tccaccgccg ccgccgttgt ggaacggcgg ttttgccagc catatgacag gaatcatgat
      961 gatgaacagc aggctgcctg ccataaagat ttcattcgat ccgatgatga agccctgctg
     1021 ggtaatggta ttgttgatga tacctagggt ttgaatgtcg gaaatgcctt gttgggacaa
     1081 atgcgccgcc gtttcgtgca atgttgcgga atagggcgtg atgtgttcgg caaagcgtgt
     1141 gtggtgcaac gcttcgcgcc gttcccacag ggtactgacg aatgatacgc cgacaccgcc
     1201 catcagcacg cgcaagaaat tcgacaggct gccggcggcg gcaatctgtc cgcccttcat
     1261 atgcgacagt gtgatggtgg tcagcggcag gaagaacatg gcaacgcctg ccccctgcca
     1321 aaactgcggc cagatgacgt tgccgatatc catgtcggca taaaaatcgg tgcgccaata
     1381 gaaagtgaag gcaaaagtca ggaagctggc ggttacgagc aggcgcatat cgattttatt
     1441 gccgaacctg ccgataatgg gggacaggaa aactgggagg atgccgacgg gcgcggcggc
     1501 aagccctgcc cacgtggagg tatagcccag gttggtctgc aacactaacg gcagcagggt
     1561 cagcgtcccc atatacacca taaaacccaa tgacgtggca atgacgccga cggtaaaatt
     1621 ccgatcttta aacagcgata aatcgacaat cggatatttt tctcccaatt cccaaacaat
     1681 aaaatacgac aagcacacca atgcggttac gcccaaggta atgatttctc ccgaagcgaa
     1741 ccagtcgagt tccttacccc tgtccagcat catctgtaac gcgccgatac cgactaccat
     1801 caatgtaagc ccgacatagt cggtcggcat tttaacggtt tccgtttccc gatatttcaa
     1861 atgtttccat gtaatccatg ccgatatgat accgataggg atattaatga agaaaatcca
     1921 accccaatgc cagtttccgg aaatccaacc gccgagtatc ggcccgagaa caggggcgac
     1981 aacgacggtc attgcccaca acgccagtgc cagcgtccgt tttgcgggcg gataggatgc
     2041 cattaacagg ctttgcgaca agggaatcag cggcccggcg ataaagccct gcaagatgcg
     2101 gaaaataacc agcgactgaa ggttgggcgc gatgccgcac agccacgatg tgatgacgaa
     2161 accgacagcg gcggcggtaa acaatttgac ctcgccgata cgttttgcca aaaagcccgt
     2221 taacggcacg gaaacggcgt ttgccacagc aaaagaagta atgacccacg tcccctgagt
     2281 ggttgccgca ccgaggttgc cggcgataac gggaacggcg acattggcga tagtcgtatc
     2341 taaaacttcc ataaatacgg caagccccaa agacagcgta atccatgcca atgccgcacc
     2401 cttaagcggt ggataatcca ttgtgtcctc cgtttcagac ggcatcaggg cgcggattgc
     2461 ccgaggattt catcgaccgt ccgatcgact tccgaccagt cggtactttc catttccggc
     2521 aatgccgcac ccggcgtttt tgaaacaggc gcgcctgcgg cggaagtatc cactttaacc
     2581 gtcatcgaca aaccgatacg caacggatgc ctgtccacat cttcgcggtt cagcacgata
     2641 cggacgggga cgcgctgcac cactttaatc cagttgcccg ttgcgttttg cgccggaatc
     2701 agcgaaaacg cgctgcccgt acctgccgaa aaacctgcca ccctgccgcg ataaacaatt
     2761 tgtttgccgt acaaatcgga caccagctcg gcaggctgtc cgattttcat atgccgcaac
     2821 tgcgtctctt taaaattggc atccacccac acatccgaca gcggcaccac cgccatcagc
     2881 ggcgcgcctg ccgccacctg ctgcccgacc tgcaccgaac gcttcgccac ctgaccgtcc
     2941 gccggcgcgc ggatttgcgt ccgctgaagg ttcaaccacg catctttcaa cctgccgatt
     3001 gccgtctgaa ccgccggctg ttcgcgcaaa gaaacctgac cgcccaaagc cgcgcgtgcc
     3061 gaagactctt ccgccaaagc cgccttaact gccgcctgcg cctgagacac tgccgcacgg
     3121 gcgtgtgcca gctcttcggc ggacaccgcg cccgattccg ccaaagcaga ccggcggcgc
     3181 aaatcatcct gcgcgcgtgc caaatccgca cggcgcaagg caacctgcgc ccccgcctgc
     3241 gaagtggcgg cattttgccg gcggttttgc cgcaccgcct gaaccagctc gttttttgcc
     3301 cgctcgtaag ccagcacatc attatcgtcg tccaataccg ccagcacgtc gccttttttc
     3361 acggcatccg tatcgtcgtg caaaaccttc cgtaccgtac cgcccttttg cggcgtaacc
     3421 tgaaccacgc gtccggcaac ataagcgtct tccgtttcct cttcgtgctg ccaccataaa
     3481 aaaaacgccg acccggcggc tgcggcggca agcgcgaaca gcagcgtcaa tgccgtcagg
     3541 cggcgtttac gtttgacttt tgcgctattg gcttcttgtc tgttttgaat tgtcgtttca
     3601 tctgcatgtg ttgccattat tccaattcca aatacttcta atactatgtt tttgatttaa
     3661 ttagcactga taatcttcaa attcccacga aaaaaaacga agtaaataag tcaatgactt
     3721 ttcccaagtt tcttttgaac attctttaag aattttctca atttccgatt taataacaga
     3781 atgattaaat tcattcataa tcatcatacc cgctccccat ttaacccttt gattttggaa
     3841 acaattatgc aaaatccatt taggagagca tatgcgaaca gaaaatatat ctgcagcatc
     3901 actatcatca gttcctatgt ctaaatcaat tcccacacaa aaattgtctt tgatttcggg
     3961 aacgaaatct tcaaaggcac aatcgtaaag attgatggct ttcaattcta ggttaatcat
     4021 tttatattca aaataaataa aaccaattaa agattggtta ggattctata ccctggcaat
     4081 aactcaatca agttaatgat ttagaatgaa tattcaaata aactgtatca gcccggcctc
     4141 acagaactat ttatatccac acatcaccga gtaaagcaat tacccatact aaccgaagaa
     4201 aactacacaa ataccgccac ttatattaca atcgccgccc gtggttcgaa aacctcccac
     4261 actaaaaaac taaggaaacc ctatgtcccg caataccgaa gagctgcaag gcatctcgct
     4321 tttgggtaat cagaaaaccc gatatccgac cggctacgcg cctgaaattc tcgaagcatt
     4381 cgacaacaaa catcccgaca acgactattt cgtcaaattc gtctgcccag agttcaccag
     4441 cctctgcccc atgaccgggc agcccgactt cgccaccatc tacatccgct acattccgca
     4501 catcaaaatg gtggaaagca aatccctgaa actctacctc ttcagcttcc gcaaccacgg
     4561 cgattttcat gaagactgcg tcaacatcat catgaaagac ctcattgccc tgatggatcc
     4621 gaaatacatc gaagtattcg gcgagttcac accgcgcggc ggcatcgcca ttcatccttt
     4681 cgccaattac ggcaaagcag gcaccgagtt tgaagcattg gcgcgtaaac gcctgtttga
     4741 gcacgacgca caataaacca tccttcccat atttcagacg gcattccttc gatacgatat
     4801 gccgtctgaa cgcctttccg aaaggttttt ttatgtacgc atttaccgac gcacagcaac
     4861 agaaggcact cttctggctg gtgctttttc atatcctcat catcgccgcc agcaactatc
     4921 tggtgcagtt ccccttccaa attttcggca tccacaccac ttggggcgca ttttcctttc
     4981 ccttcatctt ccttgccacc gacctgaccg tccgcatttt cggttcgcac ttggcacggc
     5041 ggattatctt ttgggtgatg ttccccgccc ttttgctttc ctacatcttt tccgttttgt
     5101 tccacaacgg cagttggaca ggcttgggcg cgctgtccga attcaacacc tttgtcggac
     5161 gcatcgcctt agccagcttt gccgcctatg cgctcggaca aatccttgat atttttgtat
     5221 tcaacaaatt acgccgtctg aaagcgtggt ggattgcccc aaccgcatca accgtcatcg
     5281 gcaacgcctt ggatacgttg gtatttttcg ccgttgcctt ttacgccagc agcgatggat
     5341 ttatggcggc aaactggcag ggcatcgcct tggtcgatta cctgttcaaa ctcaccgtct
     5401 gcggtctgtt tttcctgccc gcctacggcg tgattctgaa tctgctgacg aaaaaactga
     5461 cgaccctgca aaccaaacag gcacaagacc gccccgcgcc ctcgctgcaa aatccgtaaa
     5521 acagccctgc cgggagtcgg gacggttggg ttttctttta tttgcaaaca ctgtcggaac
     5581 ggataaatcc ggattcccgc ccacttcgtc attcccgcga aagcgggaat ccggtgcgtt
     5641 gggtttcagt catttccgat aaactcctgc cgcgttgtat ttctagattc ccgctttcgc
     5701 gggaatgacg gcggatgggt ttccgtgccg tggtgtccgg attcccgccc gtacgggaat
     5761 gacggcagtg cttccttggc ttaattttat tttaatcgac tgtat
//
LOCUS       DAWHXS010000117         5713 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWHXS010000117 DAWHXS010000000
VERSION     DAWHXS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.117"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            170..4003
                     /locus_tag="WIE22_001788"
     CDS             170..4003
                     /locus_tag="WIE22_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ4127826.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALIREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKVRYGSA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            4080..5081
                     /locus_tag="WIE22_001789"
     CDS             4080..5081
                     /locus_tag="WIE22_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ4127827.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(5143..5325)
                     /locus_tag="WIE22_001790"
     CDS             complement(5143..5325)
                     /locus_tag="WIE22_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127828.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            5512..>5713
                     /locus_tag="WIE22_001791"
     CDS             5512..>5713
                     /locus_tag="WIE22_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ4127829.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMLKAVLLG"
BASE COUNT         1487 a         1675 c         1405 g         1146 t
ORIGIN      
        1 ataatattgt caacaatttc tcaaagcgtt attttgtttc tataagggta tttcctgttt
       61 cggcattgaa aagtatcaaa aattgaacta cattatcgcc ttttcaaact cgcctgaaac
      121 cgacttttca gacggcattc aaataaaaac tgccaaacac ggacacacca tgaccacgac
      181 taccgcccct cagcgtattc gggaaatccc ctacaactat acttcctaca ccgaccgcga
      241 aatcgtcatc cgattattgg gcgacgaggc gtggcagatt ctgcaagact tgcgcggaca
      301 gcgtaaaact ggacgttcgg cgcggatgct gtttgaagtg ttgggcgata tttgggtggt
      361 cgtgcgcaat ccgtatctgg tcgatgattt gctggagcat ccgaaacgcc gcgccgcgtt
      421 gatacgcgaa atgcgccacc gcctgaacga aatccgcaaa cgccgcgacg ataatcggca
      481 agtggatgtc ttggttgccg cagcagaaaa agcagtcgag cgttttgata gcagttttga
      541 tgaaaccagc caaaagcggc ggcagatttt ggagcgtttg agcaaaatca ccaagccgca
      601 caatattatg tttgacggat tggcgcgggt aacgcacgtt accgatgcaa ccgactggcg
      661 cgtggagtat ccgtttgtcg tcgtcaatcc cgacacggag gcggaaatcg cgcctttggt
      721 gcgcgcctta atcgagctgg atttggtcat tattccgcgc ggcggcggca cgggttatac
      781 cggcggcgcg attcctttgg acgcaaacag cgcagtcatc aataccgaaa aactcgacaa
      841 gcatcgtggt gttgaatacg ttgagctggc aggcttggac ggcaagcatc cgattatccg
      901 gtgcggcgcg ggcgtggtta cgcggcgggt ggaagaaacc gcgcatcagg caggtttggt
      961 gttcgccgtc gatccgactt ccgccgatgc gtcctgtgtg ggcggtaatg tggcgatgaa
     1021 cgcgggcggt aaaaaagccg tgctgtgggg gacggcgttg gacaacctcg cctactggca
     1081 gatggtcaac ccgcagggcg aatggctgcg catcgagcgc gtgcgccaca atttcggcaa
     1141 aatccacgac gaagaaaccg ccgtgttcga cgttcacacg ctggattcag acggcctcaa
     1201 tatcgttaaa accgaacgtt tggaaattcc cggccacaaa tttcgcaaag tcggtttggg
     1261 caaagacgtt accgacaaat tcttgagcgg cctgcccggc gtacagaaag aaggcacgga
     1321 cggcatcatc accggcgttg ctttcgtgtt gcacaaaatg ccgaaataca cgcgcactgt
     1381 gtgtatggag tttttcggca cggtcgccac cgctacgcct tctatcgtcg aaatccgcga
     1441 tttcctgctc gcccacgaca gcgttcggct ggcaggtttg gaacatttgg actggcgtta
     1501 tgtccgcgcc gtcggctacg ccaccaaagc ggcaggcaaa ggccggccga aaatggtctt
     1561 actggcggac gtggtttcag acgacgaagc cgccgtagag gcagccgccg agcacatctg
     1621 tgaacttgcc cgcgcccgcg acggcgaagg ctttatcgcc gtatcgcccg aagcgcgcaa
     1681 aaccttctgg ctcgaccgca gccgcaccgc cgccatcgcc aaacacacca acgcctttaa
     1741 aattaacgaa gacgtggtca tcccactcga aaggctcggt gagtattcag acggcatcga
     1801 acgcattaac attgagcttt ccatccaaaa caaactcaaa ctctgtgccg ccttggaaca
     1861 atatctgtcc ggcaaactcc ccatcgacaa aatgggaacc gacctgccga ccgccgaact
     1921 tttgggcgaa cgcggcaaac acgcccttgc ccacgtttcc gccgtcaaag cgcgttggga
     1981 atggctgctt gctaacttag acgcgccgct tgccgactat aaagtacgct atggctcagc
     2041 cgtccacgcc gcgcccgaag ccaaagacaa tgaaagctgc tttaccgcat tccgcgattt
     2101 ccgcctgcgc gtgtctgtca aagcggacgt aatgaagccg ctttctgaaa tcttcagcgg
     2161 caaaaccgac accaaaatta tccaaggctt gggaaaaatc cacgcaaaaa ccgtacgcag
     2221 ccgcgtcttt gtcgccctgc atatgcacgc cggcgacggt aacgttcaca ccaatattcc
     2281 ggttaactca gacgatgccg aaatgcttca gacggcatac cgctcagtcg aacgcattat
     2341 gaaaatcgcc cgttcgctcg gaggcgtgat ttcgggcgaa cacggcatcg gcatcaccaa
     2401 gctcgaattt ttaagcgacg aagaaatgca gccgttttgg gactacaaaa accaagtcga
     2461 tcccaaacac accttcaacc gtcacaaact gatgaaaggc tcggacttac gcaacgccta
     2521 cacgccgtct ttcgagcttt tgggcgcgga atcgctgatt atggaaaaat cagacctcgg
     2581 cacgattgcc gattccgtca aagactgcct gcgctgcggc aaatgcaaac ccgtctgctc
     2641 cacccacgtt ccgcgtgcca acctgctgta cagcccccgc aacaaaatcc tcggcgtggg
     2701 tttgctgacc gaggccttct tatacgaaga acaaacccgt cgcggcgttt ccatcaaaca
     2761 ctttgaagaa ctcatggaca tcggcgacca ctgcaccgtg tgccaccgct gcgtcaaacc
     2821 ctgccccgtc aacatcgact tcggcgacgt aaccgtagcc gtccgcaact accttgccga
     2881 ttccggccac aaacgatttg cgcctgccgc agctatgggt atggcgtttt tgaacgccac
     2941 cggcccgaaa accatcaaag cccttcgcgc cgccatgata caaaccggct tccccgcgca
     3001 aaactttgcc tacaaaatcg gcaagcttct tccgatcggc acgaaaaagc aaaaagccga
     3061 acccaaggca accgtcggca aagccccgat taaagaacag gttatccatt tcatcaaccg
     3121 cccactgccc aaaagcgtac ccgctaaaac accgcgctcc ttattgggca tcgaagacgg
     3181 caaaagcatc cccatcatcc gcaaccccgc cgcgcccgaa gatgccgaag ccgtgttcta
     3241 cttcccgggt tgcggttcgg aacgcctgtt cagccaaatc ggacttgccg ttcaagccat
     3301 gctctggcac gtcggcgtac aaaccgtcct gccgcccggc tatatgtgtt gcggctatcc
     3361 gcaagacgcg ggcggcaaca aggctaaagc cgaagaaatg agcaccaaca accgcgtggc
     3421 tttccaccgt atggcgaaca ccctcaacta cctcgacatc aaaaccgtcg ttgtcagttg
     3481 cggcacttgt tacgaccagc tcgaaaaata ccgcttcgag gaaatcttcc ctggctgccg
     3541 catcatcgac atccacgaat acctgctcga aaaaggcgtg aaactcgacg gcgtgaaagg
     3601 tcagcaatac ctctaccacg acccctgcca caccccgatt aaaaccatga acgccaccca
     3661 aatggccagc agcctgatgg gacagaaagt cgttttaagc gaccgctgct gcggcgaatc
     3721 cggtatgttt gccgtcaaac ggccagacat cgccactcag gtcaagttcc gcaaacaaga
     3781 ggaaatcgag aaaaacctca aagagctgcc gcagggcgaa cccgtcaaaa tgctgacttc
     3841 ctgccccgcc tgcctgcaag gtttgagccg ctacgccgac gacaacaata tgcctgccga
     3901 ctacatcgtc atcgaaatgg cgaaacacat tctcggcgaa aactggctag acgagtttgt
     3961 gaaaaaagcc aacaacggcg gcgtggaaaa agtattgctg taacaacgga cacggaaatg
     4021 tcgtctgaac gccgaaagcc ttcagacggc attgtttaaa cccaatacct acccaaatca
     4081 tgattgacgg acaaacaacc gaacccaaac aaaaaacccg aatcatcctt gcccctatgc
     4141 agggtctggt cgatgacgtg atgcgcgacc tgctgacgcg tattggcggc tacgacgaat
     4201 gcgtcagcga atttgtacgc attacccata ccgtgcattc ccgatccata tggttaaaat
     4261 atgtccccga aatcgccaac ggaaacaaaa cgttttccgg cacgccctgc accgtccaac
     4321 ttttgggcag cgatgcggac aatatggcgg cgaatgcgct ggaagccgtc cgcttcggtg
     4381 cgaacaaaat cgatttgaac ttcggctgcc ccgcgcccac cgtcaacaaa cacaaaggcg
     4441 gcgcaatcct tttaaaagag ccggaactga tattccacat cgtcaaaacg ctgcgcggac
     4501 gtttgcccgc acatattccg ctcaccgcaa aaatgcggct cggttacgaa gacaaaagcc
     4561 gggctttgga atgcgcctgt gcgattgccg aagggggcgc atgcggactg accgtacacg
     4621 cgcgtaccaa agccgagggt tacgaaccgc cggcgcattg ggaatggata aggaaaatcc
     4681 gagacagcgt caatattccc gttaccgcca acggcgacgt tttcagcctg caagactata
     4741 tcggcatcaa aacaatcagc ggctgcaaca gcgtgatgct cggtcgcggc gcggtcatcc
     4801 gccccgattt ggcgcggcaa atcaagcaat acgagaacgg cgggccggtc aaagacacgg
     4861 attttgccga agtttccaaa tggatacggc agtttttcga gctgtgcctg acaaaagagg
     4921 caaacaataa atacccgatc gcccgtctta agcaatggtt gggcatgatg aaaaaaacgt
     4981 tcgacccggc gcaaacgctg tttgaccgcg tccgaacggt taaggatgcg gatgaagtcc
     5041 gccgaatcct gaacgcattt gagcatgaga taaatgtttg actgtctccc atgcttcaaa
     5101 gcaaatgccg tctgaaaatc tttcagacgg catttcatat ctttattgct gcttttcttc
     5161 cgtatccggc gtatttttgt ttttcagctt cgcacccaag cccaaacgtt tttcataatc
     5221 cgattgcggg gtatcgtctt cctgcatacc gaatgcgccg acattgaccc acagcgacag
     5281 cagcgcgacg acaaaggagc aaaagccgat tacataccaa aacattgccc ctcccgattt
     5341 gttaaaatca tatcaaatac agtgccgaat ttatcacaaa tgcacgggca aatatagtga
     5401 attaaattta aataaggaca aggcggcgag ccgaagacag tacaaataga gacctttgca
     5461 aaattcccca aaatccccta aattcccacc aagacattta ggggatttct catgagcacc
     5521 ttcttccagc aaaccgccca agccatgatt gccaaacaca tcgaccgctt cccactattg
     5581 aagttggatc aggtaattga ttggcagccg atcgaacaat acctgaaccg tcaaagaacc
     5641 cgttacctcc gagaccaccg cggtcgtccc gcctatcccc tgctgtccat gctcaaagcc
     5701 gtcctgctcg gac
//
LOCUS       DAWHXS010000118         5535 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWHXS010000118 DAWHXS010000000
VERSION     DAWHXS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.118"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(59..880)
                     /locus_tag="WIE22_001792"
     CDS             complement(59..880)
                     /locus_tag="WIE22_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ4127830.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            1291..3675
                     /gene="ppsA"
                     /locus_tag="WIE22_001793"
     CDS             1291..3675
                     /gene="ppsA"
                     /locus_tag="WIE22_001793"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ4127831.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            3894..5153
                     /gene="rho"
                     /locus_tag="WIE22_001794"
     CDS             3894..5153
                     /gene="rho"
                     /locus_tag="WIE22_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ4127832.1"
                     /translation="MHVSELQTLHISKLLELAEEHAIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            5459..>5535
                     /locus_tag="WIE22_001795"
     CDS             5459..>5535
                     /locus_tag="WIE22_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4127833.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTART"
BASE COUNT         1452 a         1612 c         1298 g         1173 t
ORIGIN      
        1 tgccgtctga aacctgattt cagacggcag ttccgccttc aaaccgaatc aaagcccgtc
       61 aaaacctgcg tttgagcttg cacgcctgaa ggatgtgtac cgccaattcc tcaaccgatt
      121 tatccgtcgt attcgcaaac ggaatgccat gccgtctgaa catactctgc gcgtccgcca
      181 cctcgctgcg gcatgtatcg attttggcat aagttgaatt cgggcggcgc tcttggcgga
      241 tggcctgcaa acgttccggc tggatggtca acccgaacag cttatcccta taaggcttga
      301 ccatacgcgg cagatcggcc gattccaaat cgtcgggaat cagcggatag tttgccgcac
      361 ggatgccgta ttgcagggcg aggtaaaggc aggtcggcgt tttgcccgaa cgcgatacac
      421 ccatcaagat tacatccgct tcctgaaggt tcttatcgct gaccccgtca tcgtggttca
      481 atgagaaatt caccgcctcc atacgcgcat cgtaacgctt cgtattaccg atactgtgat
      541 gcccctgctc ggatgccgtg gcttcggtat tgagttcttt ctccaacagt cccaaaaaag
      601 tctcaaagaa attaatctga aaagcatccg cccctttgat aatccgacgg atttcgtcat
      661 caaccacact gacaaacgca atcggacgct gaccgttttc ctgccggctc cgattgacct
      721 tctccaccac cgcgcgcgcc ttttccggcg tatcgacaaa cggatgcgta tggcgtttga
      781 acgacagatt gccaaactgg ttcagcaaag cctcgccgat attctcggca gtcagaccgg
      841 tacggtcgga aatgtaaaac acatggcgcg gactgctcat cttccatcct taaatacaga
      901 ataaaaatat catcataaca gcagcggcag acacaaggaa agcacccgca gcacgcctac
      961 ctcggattac acccaaacag acacaatata attttgaaat aaaattattt atataaagta
     1021 ttttttggca gaaaatttta aaaaataaac aaaaaaatca aacagaaaaa acattaacct
     1081 attcaaacca cctgttttac aaagaaaata cccaaaaaaa gaagtatacc ggctgcaagt
     1141 ttcaaaccgc tacacacgcc gaaaccgcaa tttttcagac ggcatcatga ttttaaaatg
     1201 gataaaacac atgacagcag aggaacgaat cgcttaaaat aagcacgcgg atttgtttct
     1261 tttttaacat attttggatt ggacacacaa atggccgaca actacgtaat ctggtttgaa
     1321 aacctgcgta tgacagatgt tgaacgcgtg ggcggtaaaa acgcctcgct gggcgaaatg
     1381 atcagtcagc tgaccgaaaa aggcgttcgc gtccccggcg gctttgccac cacagccgat
     1441 gcctaccgcg cattcctcgc acacaacggt ctgagcgaac gcatttccgc cgcactggca
     1501 aaattggatg tcgaagacgt tgccgaactg gcacgcgtcg gcaaagaaat ccgccaatgg
     1561 attttggata cgcctttccc cgaacagctc gatgccgaaa tcgaagcggc atggaacaaa
     1621 atggttgccg atgccggcgg cgcggacatt tccgttgccg tacgttcttc cgcaaccgcc
     1681 gaagacctgc ccgacgcatc atttgccggc caacaggaaa ccttcctgaa tatcaacggc
     1741 ttggaaaacg ttaaagaagc gatgcgccat gtattcgcct ccctgtacaa cgaccgcgcc
     1801 atttcttacc gcgtccacaa aggcttcgaa cacgacatcg tcgccctttc cgccggcgtg
     1861 cagcgtatgg tgcgttccga cagcggcgcg tccggcgtga tgttcaccct cgataccgaa
     1921 tccggttacg accaagtcgt ctttgtcact tcatcctacg gtctgggcga aaacgtcgta
     1981 caaggcgcag tcaatcccga cgaattctat gtgttcaaac ccacgctgaa agcaggcaaa
     2041 cctgccatcc tgcgcaaaac catgggctca aaacacatca aaatgatttt taccgacaaa
     2101 gcagaagccg gcaaatcggt aaccaacgtc gatgtccccg aagaagaccg caaccgcttc
     2161 tccattaccg acgaagaaat caccgagttg gcgcattacg cactgaccat cgaaaaacac
     2221 tacggccgcc cgatggacat cgaatgggga cgcgacggct tggacggcaa actctacatc
     2281 ctgcaagccc gtcccgaaac cgtcaaatcc caggaagaag gcagccgcaa cctgcgccgc
     2341 ttttctatca acggcgaaaa aaccgtcttg tgcgaaggcc gcgccatcgg tcagaaagtc
     2401 ggtcagggca aggtacgcct gattaaagat gcttccgaga tggattccgt cgaagccggc
     2461 gacgtactgg ttaccgacat gaccgaccca gattgggaac cggtgatgaa acgcgcttcc
     2521 gccatcgtga ccaacagggg cggacgtacc tgccacgccg ccatcatcgc gcgcgaactg
     2581 ggtattcctg ccgttgtcgg ctgcggcaat gcaaccgaat tgctgaaaaa cggtcaagaa
     2641 gttaccgtat cctgtgccga aggcgatacc ggcttcatct actccggcct gctggacgta
     2701 caaattaccg atgttgcttt ggacaacatg cctaaagcac ctgtaaaagt catgatgaac
     2761 gtcggcaatc ccgaactcgc attcagcttc gccaacctgc ccagcgaagg catcggcttg
     2821 gcgcgtatgg aatttatcat caaccgccaa atcggtatcc accccaaagc cttgttggaa
     2881 tttgacaaac aagacgacga attaaaagcg gaaattaccc gccgtatcgc cggttacgcg
     2941 tcccctgtcg acttctacgt cgataaaatc gccgaaggcg tggcgacatt ggccgcatcg
     3001 gtttatccgc gtaaaaccat cgtccgtatg tccgacttca aatccaacga atacgccaac
     3061 ctggtcggcg gcaacgtata cgaaccgcat gaagaaaacc cgatgttggg cttccgtggt
     3121 gcggcgcgtt atgtcgccga caacttcaaa gactgtttcg ccttggaatg caaagccttg
     3181 aaacgcgtcc gcgatgaaat ggggttgacc aacgttgaaa tcatgattcc gttcgtccgc
     3241 actttgggcg aagccgaagc cgttgtcaaa gccctgaaag aaaacggttt ggaacgcggc
     3301 aaaaacggcc tgcgcctgat tatgatgtgc gaactgccga gcaacgcggt attggcggaa
     3361 caattcctgc aatacttcga tggcttctcc atcggctcga acgacatgac ccaactgacc
     3421 ctcggtctcg accgcgacag cggcttggtg tccgaatcgt ttgacgaacg caaccctgcc
     3481 gtcaaagtga tgctgcacct tgccatctcc gcctgccgca agcagaacaa atatgtcggc
     3541 atctgcggtc aaggcccgtc cgaccatccg gactttgcca aatggctggt tgaggaaggc
     3601 atcgaaagcg tgtctctgaa cccggatacc gtcatcgaaa cttggctgta tttggcgaat
     3661 gaattaaaca aataatcaat gcccataccc ccgtgcctga aaagcgcggg ggtatttttt
     3721 ccaagcagct ccgctcagac ggcatttcct gccgatgccc cgtccgcgat aatatttgac
     3781 acccacgcgc cgactgccta caattcccct ttctccgagc aaccgccaac ggtcagcttc
     3841 ttctttcaga cggcatctgc ctgtcttttt cctcttcaaa atacatcatt attatgcacg
     3901 tctccgaatt acaaaccctg cacatttcta aacttttaga gttggcagaa gaacacgcta
     3961 tcgaaaatgc caaccgtttc cgcaaacaag acctcgtatt tgccatcgtc cgccagatga
     4021 tgaaaaaagg cgagggtttc acctgctccg gcacgcttga aatcctgccc gacggcttcg
     4081 gcttcctccg cagcgcggac acctcctatc ttgccggccc cgacgacatc tatgtctcac
     4141 ccacccaaat ccgccgcttc aacctgcata cgggcgacac catcgaaggc agcgtccgcg
     4201 tccccaaaga caacgaacgc tattttgccc tggtcaggct tgataccatc aacggcgacc
     4261 acccggaagt atgccgccat aaaatcctgt ttgaaaacct gaccccgctg tttccgaccg
     4321 aacagttgaa gctggaacgc gacttaaagt ccgaagaaaa ccttaccgga cgcgccatcg
     4381 acctgatttc gcccatcggt aaaggtcagc gcgccctctt ggttgctccg cccaaaagcg
     4441 gtaaaaccgt gatgctgcaa aacattgccc acgccgttac cgcaaactat cccgaagtcg
     4501 aactcatcgt cctcttgatt gacgagcgtc ccgaagaagt aaccgaaatg agccgttccg
     4561 tccgcggcga agtggtttcc tccacctttg acgaaccggc gcaacgccac gtccaagttg
     4621 ccgaaatggt gcttgaaaaa gccaagcgta tggtagaaca caaaaaagac gtggtcatcc
     4681 tgctggattc gattacccgc cttgcccgcg cctacaatac cgtcgtcccc acctcgggca
     4741 aaatcctgac cggcggtgtc gatgccaacg cgctgcaccg ccccaaacgt ttcttcggcg
     4801 cggcgcgcaa cgtggaagaa ggcggttcgc tgaccatcat cgccaccgca ttggttgaaa
     4861 ccggcagccg tatggacgat gtgatttacg aagaattcaa aggcaccggc aatatggaat
     4921 tgcaccttga ccgccgtatg gcggaaaaac gcctcttccc cgccatcaac atcaacaaat
     4981 ccggcacgcg ccgcgaagag ctgcttgtgc cgaacgacca gttgcaacgt atgtggctct
     5041 tacgcaagtt cctgcacccg atggacgaaa tcgaggcagc cgaattttta atcgggaaaa
     5101 tcaaagcctc taaaaacaac gacgatttct ttgaactgat gcgcggcaaa taaacgcgcc
     5161 gcccgcatta atgcgaaatg ctgtctgaag cctgaaaatc gggtttcaga cggcactttc
     5221 attcacacgg tcggcgcagc tcctccctcc ccccgttaac ggcgcaaccg tcggcagtgt
     5281 ccgtgtccgc ttgccgaaag cgcggccttt gcaaaaccgg cttgaacgca tccgtaccgg
     5341 caatgaaaac cgatgcggga acttgattca aggttgcgcc gaaccgcgct catttttgta
     5401 taaatttggg gctgtcctag ataactaggg aaattcaaat taagttagag ttgcctttat
     5461 gagaaaaagt cgtctaagcc ggtataaaca aaataaactc attgagctat ttgtcgcagg
     5521 tgtaactgca agaac
//
LOCUS       DAWHXS010000119         5100 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWHXS010000119 DAWHXS010000000
VERSION     DAWHXS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.119"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(199..1191)
                     /gene="pdxA"
                     /locus_tag="WIE22_001796"
     CDS             complement(199..1191)
                     /gene="pdxA"
                     /locus_tag="WIE22_001796"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ4127834.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAPGEGELEVLHIPTAEAVAAGRLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGESGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1351..2778
                     /locus_tag="WIE22_001797"
     CDS             1351..2778
                     /locus_tag="WIE22_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ4127835.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            3103..4998
                     /gene="mnmG"
                     /locus_tag="WIE22_001798"
     CDS             3103..4998
                     /gene="mnmG"
                     /locus_tag="WIE22_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ4127836.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT         1152 a         1486 c         1387 g         1075 t
ORIGIN      
        1 acctttcgtc attcccgtga aaacgggaat ctagaacctc aaactttcag ataatctttg
       61 aatattgccg tcgcccgaag gtctggattc ccgcctgcgc gggaatgacg atttggaaat
      121 tacccgaaac ccaaaaacaa ctgaaaccga acagaccgga ttcccgcctg cgcggaaatg
      181 acgggtcttt tatcatcttt aaaggctgcc gcgcgccatc tcgacggcgg tctccacggc
      241 agttatcagg ctgccggaat ccgccctgcc ggttgccgcc aaatcgagcg cggtgccgtg
      301 atcgacggag gtgcggataa agggcagccc cagcgtgatg ttcacgccct gtccgaagct
      361 gtggtatttc aacacgggca gcccttggtc gtggtacatc gccaatacgg catccgcacc
      421 ttcgagcata aacggttgga acaaggtatc cgccggatac gggccggcga ggtttatccc
      481 ttcgcggcgc aggttttcca atgcggggat aatggtgtcg gtttcttcgt gtccgaggtg
      541 tccgctctcg ccggcgtggg ggttgagtcc ggcgactagg attttgggat ttttgatgcc
      601 gaatttgtgt tttaagtcgt gatgcaaaat gcgtgcgacg ctttcaatca gcggttgcgt
      661 gatggcggcg gcaacgtctt tcagcggcag gtgggtcgtt acgagggcga cgcgcaggcc
      721 tttgccggca agcatcatca caacctgccc cgtgccgctt ttttccgcta gatattcggt
      781 gtgcccgctg aaaaaacctg tgcttgcgcg cgcgtcgttg atgatgcctt tgtgcagcgg
      841 cgcggtaacg atgccgtcga aaatgccgtc tgaaatgcct gcgagcgcgg tgtccaaaag
      901 ttgcagcaca taggcggcgt tggcgggatt gagccgccct gccgcaaccg cttcggcggt
      961 cggaatgtgc agcacttcca gctcgccttc tccgggtgcg ccgcctgatt ccggatcgaa
     1021 gtcgcgcaag acgacgcttt tgcccaaggc ttcggcgcgc gcgcgcaata ggtttttgtc
     1081 gcccaatacc gcgcagcggc agggcaggcg tgcaaacgcc aagtccaaac aaatatcggg
     1141 gccgatgccg gcaggctcgc cggaagtaac ggcaaaaaca ggctgtttca tcgtgtttgc
     1201 tccaaacaaa atgcgattct aacgccgcag ccgcgcggcg atgtaaattt ttctgatttt
     1261 gttgacaatc tgctagaatg ggcgtttaca aaatttaaac cctgcttgca taccgccaat
     1321 atgtgcgagt ttcaacttta aggaagcgat atgaacgaga actttaccga atggctgcac
     1381 ggctgggtcg gcgccatcaa cgatccgatg tggtcatact tggtttatat gcttttgggt
     1441 acggggcttt tcttcaccgt aaccacgggc tttgtccaat tccgcctgtt cgggcgcagc
     1501 atcaaagaaa tgctcggcgg ccgcaaacag ggggacgacc ctcacggcat cacgccgttt
     1561 caggcatttg taaccggcct tgccagccgc gtgggcgtgg gcaatatcgc gggcgtggcc
     1621 atcgccatca aagtcggcgg accgggcgcg gtgttttgga tgtgggtaac cgccttaatc
     1681 ggtatgagtt cggcgtttgt cgaatcttcg ctggcgcagc tctttaaagt ccgcgactac
     1741 gacaaccacc atttccgggg cggccctgcc tactacatca ctcaagggct ggggcagaaa
     1801 tggctgggcg tgttgttcgc cctgagcctg attttctgtt tcggctttgt gtttgaagcg
     1861 gttcagacca ataccattgc cgataccgtc aaagcggcgt ggggttggga gcctcattat
     1921 gtcggcgtcg ccctggtgat tttaaccgcg ccgattatct tcggcggcat caggcgcata
     1981 tctaaagcgg cggaaatcgt cgtccccctg atggcggttt tgtacctctt tatcgcgctt
     2041 ttcatcattt tgaccaatat tccgatgatt ccggacgtgt tcggtcagat tttttcgggc
     2101 gcgttcaaat tcgacgcggc agcaggcggc ttactcggcg gtctgatttc gcaaacgatg
     2161 atgatgggca tcaaacgcgg cctgtattcc aacgaggcgg gtatgggttc cgcgccgaac
     2221 gccgccgccg ccgccgaagt gaaacaccct gtttcgcaag gtatgattca aatgctgggc
     2281 gtgtttgtcg ataccatcat cgtttgttct tgcaccgcct tcatcatctt gatttaccaa
     2341 cagccttacg gcgatttgag cggtgcggcg ctgacgcagg cggcgattgt cagccaagtg
     2401 gggcaatggg gcgcgggctt cctcgccgtc atcctgttta tgtttgcctt ttccaccgtt
     2461 atcggcaact atgcctatgc cgagtccaac gtccaattca tcaaaagcca ttggctgatt
     2521 accgccgttt tccgtatgct ggttttggcg tgggtctatt tcggcgcggt tgccaatgtg
     2581 cctttggtct gggatatggc ggatatggcg atgggcatta tggcgtggat caaccttgtc
     2641 gccatcctgc tgctctcgcc cttggcgttt atgctgctgc gcgattacac cgccaagctg
     2701 aaaatgggca aagaccccga gttcaaactt tccgaacatc cgggcctgaa acgccgtatc
     2761 aaatccgacg tttggtaaat cccgccctta ccagagccgc ttccccgcga agcggctttt
     2821 ccctttccgc acactgtaaa aacagggcga acaagcgtac aatcccaacc ctttactttt
     2881 gaatccattt cgtttttcag acggcatatt gaatatagtg gtttaacaaa aatcgggaca
     2941 aggcggcgaa gccgcagaca gtacagatag tacagaaccg attcacttgg tacttcagca
     3001 ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ctgtactggt ttttgttaat
     3061 ccactataaa tgccgtctga aacaccgtca ggcaatacac acatgaccca catgatttac
     3121 cccaaaacct acgacgttat cgtcgtcggc ggcggacacg ccggcacaga agccgcgctc
     3181 gccgccgccc gtatgggcgc gcagacgctt ttgctcacac acaatattga aacgctcgga
     3241 caaatgtcgt gcaacccttc catcggcggc atcggcaaag ggcatttggt gcgcgaactc
     3301 gacgcgctcg gcggcgcgat ggcgttggca accgacaaat ccggcatcca gttccgccgt
     3361 ctgaacgcca gcaaaggcgc ggcagtacgg gccacgcgcg cgcaggcgga tcgtatccta
     3421 tataaagccg ccatccgcga aatgttggaa aaccaagaaa acctcgacct tttccaacaa
     3481 gccgtcgaag acgtaacgct cgatggcgac cgcatttcag gcgtaatcac cgcgatgggc
     3541 gtggagttta aagcacgcgc cgtggtgttg accgcaggca cgtttttgtc cggcaaaatc
     3601 cacatcggtt tggaaaacta cgagggcgga cgcgccggcg accccgccgc caaatcgctg
     3661 ggcggacgtt tgcgcgaatt gaacctgccg caaggccgtc tgaaaaccgg cacgccgccg
     3721 cgcatcgacg gacgcacgat tgacttctcc caactgaccg aacagcccgg cgacacgccc
     3781 gttcccgtca tgtccgtacg cggcaacgcc gaaatgcacc cgcgccaagt gtcctgctgg
     3841 attacgcata ccaacacgca aacccacgac atcatccgct caggcttcga ccgcagcccg
     3901 atgtttaccg gcaaaatcga aggcgtgggc ccgcgttatt gtccgtctat cgaagacaaa
     3961 atcaaccgct tcgccgacaa agacagccac cagattttcc tagaacccga aggcctgact
     4021 acgcacgaat actaccccaa cggtatctcc accagcctgc cgttcgacat ccaaatcgcg
     4081 ctcgtccgca gtatgaaagg tttggaaaac gcccatatcc tgcgccccgg ctacgccatc
     4141 gaatacgact acttcgatcc gcgcaacctc aaagcaagcc tcgaaaccaa aaccattgcc
     4201 ggattgtttt tcgccgggca aatcaacggt acgacaggtt atgaagaagc cgccgcgcaa
     4261 ggactgctgg caggcgcgaa cgccgtgcaa tacgtccgcg aacaagaccc gctcctgctg
     4321 cgccgcgaac aagcctatct cggcgtattg gtggacgacc tcatcaccaa aggcgtgaac
     4381 gaaccctacc gaatgttcac cagccgtgcc gaataccgcc tgcaactcag ggaagacaac
     4441 gccgatatgc gcctgaccga agacggctac aaaatcggct tggtgggtga agcgcaatgg
     4501 cgcatgttca acgaaaaacg cgaagccatc gaacgcgaaa tccaacgttt gaaaacaacg
     4561 tggtacacgc cgcaaaaact cgccgaagac gaacaaatcc gcgtattcgg gcaaaagctc
     4621 agccgcgaag ccaacctgca cgacctcctg cgccgcccga acctcgacta cgccgcgctg
     4681 atgacgctcg aaggcgcgat gccgtctgaa cgcctctccg ccgaagtggt cgaacaagtc
     4741 gaaatccaag tcaaatacca aggctatatc gaccgccaaa acgaagaaat cgacagccgc
     4801 cgcgacatcg aaaccttaaa gctgcccgag gatatcgatt acagcaaagt taaaggtttg
     4861 tcggcagaag tgcagcaaaa gctcaaccag cacaaacccg aaaccgtcgg gcaggcaagc
     4921 cgcatttccg gcgtaacccc tgcggcagtg gcattgctga tggtgcattt gaagcgcggg
     4981 tttaaagacg cgaaataaac acatcggcgc gatgccgtct gaaacaccgt tcagacggca
     5041 tcgcgccatc ccgacaggaa acatcatgca catactgacc gccggcgtgg acgaggcagg
//
LOCUS       DAWHXS010000120         4899 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWHXS010000120 DAWHXS010000000
VERSION     DAWHXS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.120"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..668
                     /locus_tag="WIE22_001799"
     CDS             <1..668
                     /locus_tag="WIE22_001799"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="component of the membrane-bound D-lactate oxidase,
                     FAD-binding, NADH independent; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ4127837.1"
                     /translation="RFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHL
                     ILKMGGKGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDD
                     EVEDLVALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCM
                     ALEHEMLHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKN
                     WAE"
     gene            800..907
                     /locus_tag="WIE22_001800"
                     /pseudo
     CDS             800..907
                     /locus_tag="WIE22_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            963..1334
                     /locus_tag="WIE22_001801"
     CDS             963..1334
                     /locus_tag="WIE22_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ4127838.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            1489..2037
                     /locus_tag="WIE22_001802"
     CDS             1489..2037
                     /locus_tag="WIE22_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ4127839.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            2171..3262
                     /locus_tag="WIE22_001803"
     CDS             2171..3262
                     /locus_tag="WIE22_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ4127840.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            3736..3906
                     /locus_tag="WIE22_001804"
     CDS             3736..3906
                     /locus_tag="WIE22_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ4127841.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         1371 a         1466 c         1170 g          892 t
ORIGIN      
        1 acaggttcgg caaaaaagcc agcttcctgc ccaaacattt ttccgacaag gccatgcagt
       61 tcgtcagcaa atacctgccc gaccacctgc ccaaatccat gcgcgattac cgcgacaaat
      121 acgaacacca cctgattctg aaaatgggcg ggaaaggcgt ggatgaggcg cgcgcgttct
      181 taaaagaata ttttgcacac cacagcggcg cgtttttcga gtgcaacgcc gaagaaaccc
      241 aagccgcgat gctgcaccgt ttcgccgtcg cctccgccgc catccgctac cgcgccgtgc
      301 atgatgacga agtggaagac ttggtcgcgc ttgacatcgc cctgcgccgc gacgaccgcg
      361 actggtttga aaaactgccg cccgaaatcg acaataaaat catccacaaa ctctactacg
      421 gacatttcat gtgccacgtt ttccatcagg attacatcat caaaaaaggc aacgactgca
      481 tggcgttgga acacgaaatg ctgcatctct tagaccaacg cggcgcgcaa tatcccgccg
      541 aacacaacgt cggtcatttg tatgaagcca agcccgcgct caaacagttt taccgcaaac
      601 tcgacccgac caacagcttc aacccgggta tcggcaaaac cagcaaaaag aaaaactggg
      661 cggaataagc gcgtccgctt tgaaggcagg caatgccgtc tgaaggcaaa acgccgttca
      721 gacggcattg tcgggtttga agttccccgt cgggtaatcg ctttcaaacc gtatgccgac
      781 acacgggcgc gcccctccca aatatagtgg attaaattta aatcaggaca aggcaacgaa
      841 gccgcagaca gtacagatag tacggcaagg cgaggtaacg ccgtactggt ttaaatttaa
      901 tccactataa agtctgatcc taccttccca aagggcaggg tttcaaccga aaaggaaaca
      961 cgatgaaacc atacaaaatc tacacccatc ccgccctgcc gccccaagcc gtcaaacaag
     1021 gctggtcatg gccggggctc ttgttcggca cgctgtgggc gtgtttcaaa agaatgtggg
     1081 gtttggggct ggggctgacg ggcgcgatat tcgtgctggc tgtgtttgcc caactggttt
     1141 acggcgacac gcccgccaca gactcggcgt ttaacgtatt gggcttggcg gtttccgtct
     1201 ggttcggcgc aaagggcaac agcctttatg cacgccacct tttatcgcgc ggctataccg
     1261 aattgcccga aacggttgag gcagccaatc cccaagccgc attggcgcaa tatttcgggc
     1321 gcgggggccg ataagcgcgt tttcttttcc atcatgccgt ctgaaaaatg ttcagacggc
     1381 atttcttata accttttgtt tgcagcacat aaccaatcag tctttcctat tccgttccga
     1441 aatgcatacc atgcgcccgt acccaatatt tgcgaacaag gaaagaaaat ggcacgttta
     1501 accgtacaca ccctcgaaac cgcccccgaa gccgccaaag cgcgcgtcga ggcggtactt
     1561 caaaacaacg gctttatccc caaccttatc ggcgtattat caaacgcccc cgaagccttg
     1621 gcgttttacc aagaagtcgg caagctcaac gccgccaaca gcctgaccgc cggcgaagtc
     1681 gaagtcatcc agatcatcgc cgcccgtacc aaccaatgcg gtttttgcgt ggcagggcac
     1741 accaaactcg caaccctgaa aaaactcctg tccgaacaat ccgtcaaagc cgcgcgcgct
     1801 ttggcagcag gcgaatttga cgatgccaaa ctcggcgcgc tcgccgcctt tacccaagcc
     1861 gtaatggcga aaaaaggcgc ggtatccgac gaggaactca aagcattttt cgatgcgggc
     1921 tacaaccagc agcaggcagt cgaagtcgtg atgggcgtag ccttggcaac cctgtgcaac
     1981 tacgtcaaca acctcggaca aaccgaaatc aaccccgaat tgcaggctta cgcctgatac
     2041 ggcaaaccgc ccgaatatcg ggcggttttt caaaaacacc ccctcaaaca aaaacaagcc
     2101 gccaagcggc agggaagcag ccgcgcatca ggcgcgcccc cgattccccc gcaaacctta
     2161 aggaacaaag atgaacgccc aaaccctgat tgccaacgtt gccgaattcg tcaaaaccaa
     2221 gctcaaaccc atagtggacg acatcgaccg caaaggatac taccccgaag catttatgcg
     2281 cgaactcggc gcaatcggtg gattcggcgc agtcggtaca gaagccgaag gcggcaacgg
     2341 cttgggtttg gcaacgcaaa tcgccgtatt gcgcgaaatc ggcaaagaat gcggcgcaac
     2401 ctctttcagt gcgtggtgtc aggcggcttg cgcgtggtat ctgcaccaaa cgcccaaccg
     2461 ggccgtcaaa gacaaatacc ttgccgacat cctgcaaggc aaagtattgg cgggtacggg
     2521 catgtccaat accgtcaaac accttgccga catcgaaaaa cacaacctcc aagccgagcg
     2581 cgtggagggc ggctacacag tcaacggcgc gctgccgtgg gtgtccaaca tcggcgaaga
     2641 ccacatttgg gcgaataccg cccaaatcgg cggcggctac gtcatgttca tcacaggcgg
     2701 acaatgggaa ggcgtaagcc tgcaaaactg ccccgaattt tgcgccctcg aaggcacgcg
     2761 caccttcagc ctgaacttca aagacgtatt tatccccgac gaagacatca tcgccgcgcc
     2821 cgaacagttt gccgactaca tccaaagcat caaagcaggc tttatcctcc tgcaaatcgg
     2881 catcggcgcg ggcgtgatag acggcagcct cggcatcatc cgcctcgcca acgtcgtcaa
     2941 cgccgaagtc aaccgctatc tcgacgacgg ctacgacagc ctcaaaacaa ggctggacgg
     3001 cgcgtgggca gaaaccgaac ggctcgccgg attggcttgg agcggcacgc ccgacaacct
     3061 taccaccctc aagctgcgcg aagccgccgc cgtactgaca cttgccgccg cccaatccgc
     3121 cgccctgcat tccggcgcaa aaggctacct gatgcggagt cccgcccaaa ggcgcgtccg
     3181 cgaagcaatg tttgtcgcca tcgtaactcc cgcgatcaaa cacctccgca aagaaatagc
     3241 ggcaatcgaa gccgcaaaat aaacaggcgg aaaaaaccga aacccccatc gcgtcattcc
     3301 cgcgaaagcg ggaatccagc ccccaacgta acaggaatcc atcggaaaaa acagaaaccc
     3361 tcaaaccgtc attcccgcaa aagcgggaat ccagcccccc ctaacgcaac aggaattcat
     3421 cgaaaaaacc gaaacctcca aaccgtcatt cccacggaag tgggaatcca gtaaccgaaa
     3481 aaccacagga atctatcgaa aaaaaaccca acccccaaaa aaaccgggcg gatacctcac
     3541 catcctccca aaccctgatt tatagcggat gaacaaaaat cagggcaaga cggcgaaacc
     3601 gcagacagta caaatagtac gaaaccgatt cacttggtgc ttgagcacct tagagaatcg
     3661 ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttcatccg ctatacaaaa
     3721 aacaaggaaa cacaaatggc gcaatatatg tgcggcccct gcggctggat ttacgatgaa
     3781 gaacacggcg acccagaaca cggcatcccc cccgggacaa agtttgaaga cattcccgac
     3841 gactggaaat gccccgaatg cggcgtgggc aaagaagatt tctacctgtt ggatttcgtg
     3901 atatagcggc agccgcaccc ttagggaaaa ccccgtcatc cggcgttccg cctgcggcgg
     3961 cagggaacgc cgttttatag tggattaaca aaaatcatga caaggcgacg aagccgcaga
     4021 cagtacaaat agtacggaac cgattcactt ggtgcttcag caccttagag aatcgttctc
     4081 tttgagctaa ggcgagccaa cgatgtactg gtttttgtta atccactata tccgtttgcg
     4141 cccgccaagc cgtgttgcac agatgcgaca cggctttttt ctttctgttg tcagaatgta
     4201 aattttcgtc aaaaaacatc aaaaatcacc ccccccccga atttgcgtaa aataaagtgt
     4261 aatccctttg atcataaggg aatgttgaca attagatcga ttctcctagg ctgactttcg
     4321 caataaatgg ggttgtcaat aattgtgtat ggtcgtttgc cgtccggcgg ccgtcattcc
     4381 cgcgcaggcg ggaatccaga aacgcaacgc agcaggactt tatcggaaaa aaccgaaatc
     4441 ccaccgccgt cattcccgcg caggcgggaa tccagcaaca caacgcagca ggactttatc
     4501 ggaaaaaacc gaaaccccac cgaccgtcat tcccgcaaaa gcgggaatcc agacccgtcg
     4561 gcacggaaac ttaccggata aaacagtttc cttagattcc acgtcctaga ttcccgcctt
     4621 cgcgggaatg acgagatttt agattatggg aatttatcag gaatgattca atccatagaa
     4681 aaaccacagg aatctatcag caaaaacaga aacccccacc gccgtcattc ccgcgcagac
     4741 gggaatccaa aaacgcaacg caacaggaat ttatcagaaa aaacagaaac cccaccgacc
     4801 gtcattcccg cgcaggcggg aatccagaaa cacaacgcgg caggacttta tcggaaaaaa
     4861 ccgaaacccc accgaccgtc attcccgcaa aagcgggaa
//
LOCUS       DAWHXS010000121         4878 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWHXS010000121 DAWHXS010000000
VERSION     DAWHXS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.121"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(456..800)
                     /locus_tag="WIE22_001805"
     CDS             complement(456..800)
                     /locus_tag="WIE22_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127842.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(838..1161)
                     /locus_tag="WIE22_001806"
     CDS             complement(838..1161)
                     /locus_tag="WIE22_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127843.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYETFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(1164..1352)
                     /locus_tag="WIE22_001807"
     CDS             complement(1164..1352)
                     /locus_tag="WIE22_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ4127844.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(1422..1757)
                     /locus_tag="WIE22_001808"
                     /pseudo
     CDS             complement(1422..1757)
                     /locus_tag="WIE22_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(1863..2234)
                     /locus_tag="WIE22_001809"
     CDS             complement(1863..2234)
                     /locus_tag="WIE22_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ4127845.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(2231..2482)
                     /locus_tag="WIE22_001810"
     CDS             complement(2231..2482)
                     /locus_tag="WIE22_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127846.1"
                     /translation="MDNTRIMAAHEAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(2614..2820)
                     /locus_tag="WIE22_001811"
     CDS             complement(2614..2820)
                     /locus_tag="WIE22_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127847.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(2830..3675)
                     /gene="mafB"
                     /locus_tag="WIE22_001812"
                     /pseudo
     CDS             complement(2830..3675)
                     /gene="mafB"
                     /locus_tag="WIE22_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(3772..3984)
                     /locus_tag="WIE22_001813"
     CDS             complement(3772..3984)
                     /locus_tag="WIE22_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127848.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(4061..4420)
                     /locus_tag="WIE22_001814"
     CDS             complement(4061..4420)
                     /locus_tag="WIE22_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ4127849.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(4425..4853)
                     /locus_tag="WIE22_001815"
     CDS             complement(4425..4853)
                     /locus_tag="WIE22_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127850.1"
                     /translation="MQTLDGEMAGGNKPPKSITSGGKANAATYPQLVNQLTGQNLKNI
                     AAQDPRLSLALHKSEKNFPIGTATYEEADRLGKIWVGEGARQTSGGGWLSIDGTRQYR
                     PPTEKNSQFATTGIQANFETYTIDSNGKRNKIKNGHLNIR"
BASE COUNT         1354 a          952 c          775 g         1797 t
ORIGIN      
        1 attaatacct tgtaaagttt gctgcgcaga gatgtaggtc ggatatttgt atccgacaaa
       61 aatatttgac gcatctatcg tttccgaaat gccgctgttg gaaaatgtcg gattcgagaa
      121 tccgacctac ggctatttcg tttaagtcat aaatagccat tcatgttcct tctgaatttg
      181 cttttgctat ccttagaacc aatcatataa acattatcat cattaattaa tgttctaagt
      241 ttatgaagcg gtcaaatctg tcaaactacc agagatgaag catatcctcc tggaagtaat
      301 tctgttcgtg ctgtttccat atcttctttg ccatcatcag ttacttctct gtctaaataa
      361 aatcttacga taagtttttg tctaatttca tcaaattctg ccgtaattaa tcttatattg
      421 atatgaatac acggttcata cctgctatta gccagtcagg tgtagttgga tggggattca
      481 tttttaataa tatcttcaag caagtgaaat gtaagaaaag cgtagatact tgaaggccgg
      541 ctatctgatt ggcaaaaaga aatttcggag ataaagaatt tccgtttata tttttcataa
      601 attaaatcaa caccttgttt tacataaaaa ctaacttcct caggaaggat atttataaca
      661 ttctctcctt ttacttttat agttaatggt ttgattttta tgttttcatg aatatctgat
      721 aggattattt ctaaaaaatt atttttattt ccagtcattc tggctatttt atgtatattt
      781 ccattgtgtt cgattatcat tttatttttt ccaaggattt ccttctatat tgatagttta
      841 tctaaagatt tcatttaaaa tatcctgact aatttcttgt ttatataatt ctacaatttt
      901 ctgtttctgt gtatttgtat aaaatttttc gttggtatct gcttgatatt ttaattgtct
      961 caatatttcc atgatttcat attctgattt ttcatcatat tcttcaatca tgtaatcaac
     1021 aaattgtttt agaatatcaa aggtttcgta actttccaaa attttaatat gtttatcggc
     1081 aaaattggac atataccatt cgtcagtgat tggattagta tttaaatcat ctaaaaaaac
     1141 atttacatca tctaaaaaca tgactaatcc ttaaacttaa aatctgtttt ctcaccggtt
     1201 cttgagtttt caatgtaatg tatttcaatt ttgtttacgt tttgaacacg cttaacccaa
     1261 ccgtcttttg ctaaccaacc atttttagta tcggacatag gaggtattct cgcaggagtt
     1321 ttgccctgtg ggtttgcctt aacttcttcc attgctaatt gttcctttaa attattaggt
     1381 attgtacgac cggttgattt ttcaacagat tgtgtcccta tttttaatga tttgtttccc
     1441 ccagccattt ccccgtctac agtctgcata gggcgggcag caggtttttt atagccggta
     1501 tttttaaccc aatcccattt agtcgggtta agttctactt ttttaccgcg ccaaaccgta
     1561 cctgcggcct ctgctacgga aagggcagtt tgggctgttg cagttatatt cggatgggca
     1621 tcagcccatt gtctggcgga attaatgccg ttttttaccg caaaagcact gtcctgtaat
     1681 aggctcgcgg cagcaagttg tgcttccgga cttaaatttc ctaattcatt aatatcttgt
     1741 aaagtttgct gcgcagagat gtaggtcgga tatttgtatc cgacaaaaat atttgacgcg
     1801 tctatcgttt ccgaaatgcc gctgttggaa aatgtcggat tcgagaatcc gacctacggc
     1861 tgctaccaac catcattttc agcttctttt agccaagtat caaaattttc aaaagtctcg
     1921 taatattttg tattatctaa attaactact ataattttag gattacctga tttatctgaa
     1981 atatcaaaac aggcagccca ctcttggttt ctagcaaaag gtaataggtt tggaatgcca
     2041 gttatttcaa aataaatgtc aattacttct gatatattgc tttctgcatt ttcaaaccac
     2101 caaggaaaag aatattgttc atcgtagtta atggcatgag tggattgagc taattttaaa
     2161 taactttgcg gatatttaaa tcctttagga agcattgctt caggatacgg aacaaaacta
     2221 ttttgtttta tcatttccta agtaccttta catcataaat acttccgtta ggaaatcttt
     2281 tgctccaccc ttctggaact cctttttgtg tttcttgctt tctaattcgt agctggatag
     2341 cttctcccca agtttgaggc tcaataccat catgcttaaa cctgattctc tcttttgatg
     2401 ataatcggtc attaaaatta tgaacattcg cttccacttt tacccctgct tcatgtgctg
     2461 ccataatacg cgtgttatcc atactagtag gtgctccatc aggcatatta acaacatcaa
     2521 ccctgtcgcc aacccatgat ttttttcgtt cattgcagct acaagagaat catagttgta
     2581 atttgcacta ggctctgaca aaaatttggg ttgttaagtt gtcatttttt tataaattcc
     2641 agcaaactgt tgttgtaaat tatctacttc atgttttcta acaccagata cattttcaaa
     2701 ttctcttgaa tctacacata cgcccgcata tatctcattc aagatagctc gtaaaacaaa
     2761 aacttcatct tgagttagtg aaatagttga gttttgatta tttatggata gtatattcat
     2821 ggctttttcc tataaatcat cataatattt tttacctgat gttggtctaa atgctgtacc
     2881 tccatcgtca gaatttttat ctcggataac tattgtccct gttttatcat cccaatacgc
     2941 agttctaccg cgaggtaact ctttcatatt tgttggatgg ctaacaatat tttcaatatg
     3001 ccgagcaaaa tctgctggtg aattgatatt taaatccgta aattcttgtt gtcttatgac
     3061 atgcttgtta taagcatgcc cactactaat ttgttctcca attagttgcg cttgtaaaga
     3121 cggttgtgtg gaagcatctg ctttgctgtt ggacgttata gatttaggcg gtctattccc
     3181 ccctgccatc tccccatcta cagtctgcat atggcgggca gcaggttttt tatagccggt
     3241 atttttaacc caatcccatt tggtcgggtt aagttctact tttttaccgc cccaaaccgt
     3301 agttgcggcc tctgctacgg caagggcagt ttgggctgtt gcagttatat tcggatgggc
     3361 atcagcccat tgtttggcag agttgatacc gtcttttacc gcaaaagcac tgtcttgtaa
     3421 tgcggttgca gccgcaagtt gtgcttcggg acttaaattt cctaaatgat taataccttg
     3481 tagagtctgc tgcgcggctg tatctgtgac cgggcttact gcactgtctg taattgcccc
     3541 aatccctact ccttgaaaac ccattaaaaa aggattaacc gcaccttgga cgatgccgcg
     3601 aacatcatcc atacggttag cccaccaatt tttattgggg tcgttttccc atatcagttt
     3661 tcctgcttca tcagcacagt taggattggc attgatgccc acccagtttc aggcagcctg
     3721 aaaaggattt ttaatatacg gcaaaaggtg ggcaacaagt gcccacctta cctagctaac
     3781 atttatccca agtatcatat atttttttaa gttctttatc caaagaaaga actttatttt
     3841 ttcaacacca tgaattatct caaaatctct acaatccacg catacgcctg aataaatttc
     3901 tcctacaatt gcttgaataa tatacaactc atctttatcg agactaattt taactgaact
     3961 ctcttcaaaa ttaattaatt tcatgaataa ttccttttat tttaattcgt catcaaaata
     4021 ccgtttgcct gtttttggtc taaatgctgt cccaccatct ttagtcttgg atatttagat
     4081 tataatcgtc aaattcccag tcaaaatatt ttgccaaatt tgatatagac ttatcttcag
     4141 acgttgaatt acatatttcc aatatatcat ttatcaattt taatatgtgt tcaatattaa
     4201 aacggtttgt aacaattgag ttttttaatg acatcggtct atgtatattt gaaagtaacc
     4261 attcagtgct atatatataa acataaaaat aatcagcacc attactttca tcaaaaccta
     4321 ttgataatgt caaaagtata caaaaatttt ctaacttgtc ttgtgggaaa tcttccaaat
     4381 caaagtcaga acaatcataa ttttttaatt ttaatctcat ataattacct aatatttaaa
     4441 tgtccatttt taattttatt cctttttcca tttgaatcaa tagtataagt ttcaaaattt
     4501 gcttgaatac ctgtagttgc aaattgtgaa tttttttctg ttggtggccg atattgtcga
     4561 gtgccatcta tacttaacca tccgcctcca ctagtttgtc ttgcaccctc accaacccaa
     4621 attttaccta gcctatctgc ctcttcataa gttgcagttc ctattggaaa atttttttca
     4681 ctcttatgaa gagctagact caatcttgga tcttgagccg caatgttttt taagttttgc
     4741 ccagttaatt gattaactaa ttgaggataa gttgcagcat tggcttttcc tccagacgtt
     4801 atagatttag gcggtttatt cccaccggcc atttccccat ctaaagtctg cataggtcgg
     4861 gcagcaggtg ttttataa
//
LOCUS       DAWHXS010000122         4799 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWHXS010000122 DAWHXS010000000
VERSION     DAWHXS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.122"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..172
                     /locus_tag="WIE22_001816"
     CDS             <1..172
                     /locus_tag="WIE22_001816"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ4127851.1"
                     /translation="DRQKPAQYVGQHPDAYLHEIAKHFDCTAATICYALKQMGITRKK
                     RPPLTKNKTRPK"
     gene            223..669
                     /locus_tag="WIE22_001817"
     CDS             223..669
                     /locus_tag="WIE22_001817"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ4127852.1"
                     /translation="MDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGN
                     RLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHK
                     VLPLAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
     gene            complement(833..2236)
                     /locus_tag="WIE22_001818"
     CDS             complement(833..2236)
                     /locus_tag="WIE22_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ4127853.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2305..3732)
                     /locus_tag="WIE22_001819"
     CDS             complement(2305..3732)
                     /locus_tag="WIE22_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ4127854.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3968..4321
                     /locus_tag="WIE22_001820"
     CDS             3968..4321
                     /locus_tag="WIE22_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127855.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4609..>4799
                     /locus_tag="WIE22_001821"
     CDS             4609..>4799
                     /locus_tag="WIE22_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127856.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSV"
BASE COUNT         1160 a         1254 c         1171 g         1214 t
ORIGIN      
        1 ggataggcaa aaaccggctc aatatgttgg gcaacacccg gatgcctatc tgcatgaaat
       61 cgccaaacat tttgattgta cggcagccac catttgctat gcactcaaac agatggggat
      121 aacgcgcaaa aaaagaccac cacttacaaa gaacaagacc cggccaaagt aacgcattat
      181 ttgacacagc cggccgaatt ttccgactac caacgtgttt atttggatga aacaggattt
      241 gaccgctacc tgttccgtcc ctatgcccgc agcctgaaag ggcaaatagt gaaagcgcag
      301 ataagtggaa aaagatacca acgcttatct ttggtgtccg cacaagtcgg caaccggctg
      361 attgctccga tggtttatca aaatacgatg accggagtct tttttgaagc gtggtttcag
      421 caatgcctac tgcccgcatt gactcaaaaa tcggtgatta ttttagataa tgcgcgattt
      481 caccgtatgg gtgtcttacg ggaaatggcg gaaaaattgg gacataaggt attgcctctt
      541 gcaccttatt cacctgagct caacccgatt gagaaagtgt gggcgaatat taagcggtat
      601 ctgcgaaccg ttttgtctga ttacgcccga tttgacgatg cactactgtc ctattttgat
      661 tttaattgaa tatacatttg aattaattcg cacttaattt aaatgtgttt ttaactgtgc
      721 tttatttaaa ggcaatgaga atgtgaaagt atcggatcaa tcccaaagca gcctgcactt
      781 tcgaaacggg gtgcaggctg ctttgggaat ttcataaccg tttcagcctg ctttattccg
      841 caaataccgt ttccaaccct aacccgctct ctttcaccaa gcgcaaataa gccagcatga
      901 atttataccg tgcttgagcc agtttctgtt ctgcttgggc gacttcctgc cgcgcccgta
      961 ttacttccag ccggttgcgg atgccgtatt gttggccggt ttcggtcgat ttcagtttca
     1021 aacggctgct ttccaaaacc cgttcttgcg ccatgatttg gtaacgcgcc gcaccgcttt
     1081 cggtataagc ttggcgtacg gcgagtttga tgtgccgctc ggttgcggtc agctgtgctt
     1141 cggcggcccc gtattgcgct tcggcttcat ggattttgcc cgacaattct ccgccggtat
     1201 aaagcggcaa attcaactgt acgccgacgc tcatcccttt gccccgatag tggtagtcat
     1261 tattctgcgc agatgaagtg tagaggttat tctgatagcc gacatgggca gaaacggtgg
     1321 gatagcggct gttctgtgct gcccgaagcg cctgtccgct gctttgcagg gcaagctgct
     1381 gcatccggta ttcatgattg ttggataagg caatgcgctg ccattcatcc agactgtaac
     1441 gttccagctt gggcagatag cgtgccaaca ggttggcggt atctatggcc tcgatttgtt
     1501 tgctatccag gtcggtgtag tcgttcaact ggttttcata ggtttgtttc tcagccaata
     1561 cggcgatttc ttgggccagg gcattgtcgt aaccggcttt ggcttcgtga atatccagcg
     1621 cggtggcagc acctttattg aataaagcct gcgcctgcct tacctgctgg gcataagcct
     1681 ctttttccgc cgcatgggcg gcaacggtgt ctcggctgag taaaacgttg aaataacttt
     1741 cggcaacttt caacagcaat tcttcgcgtg ccgcatcgaa acgctgttct gcagcctgcg
     1801 tatcgaacct gctttggcgg tattgtgcaa atttggcagc gtcaaataag gtttgtccca
     1861 cctgcacgct ccatccctgt gtttcgcggg tggaagaaat cgatggcggc tggcgctggt
     1921 agctggcatt ggcggataca tggggaagga atgcggcctt ggcttgttgt tgccgtgcgc
     1981 gcactgcatc acgctggtaa tgggacgctt gaaaatcagc cgaatgttgc tgcgccgccc
     2041 gccatgcttc aggcagcgta aaagccgaaa cggatgggga aagggatagt ggcaaggtaa
     2101 aaagtgaaac gggtaggata tatttggaaa aataggattt catagccgaa aatagttcat
     2161 gttgcaaata gggcgtcagt gtcaggcaaa cggaaatacc gtaatcttgc attatcatta
     2221 gattgagcaa tgtcatccgg gcaatggttt caggcagtct gcatgtccga accggcggat
     2281 aacaaatgcc cagtacggat ccgcctatcg ctccctaaag ctttcgtcca atttggtttg
     2341 cagcgggctt aacagataat ccagcacccg ccgtttaccc gttttaatct ccgccgtgac
     2401 attcatgccc gccgtcagat tcactgcttt gccgtcaata ttcaaggtat gtttgtccag
     2461 cgacaccacc gccgtataaa ccaagcccaa ctgttcgtgg cttaccgcat catggctgac
     2521 acttttcacc ttgcccgtca gataaccgta gcgcgtatag ggaaaactct caatcttcac
     2581 caccgcatcc tgtccctgtt ccacaaaacc gatgtctttg ttcaatacca aaacttccac
     2641 gtccattttg tcgtcatcgg gcgcaaccac catcattttt tgggcagcct gcaccacacc
     2701 gcccaccgta taggtggcca attcctgcac cgtgccgtcc gcaggcgact gtattgtcat
     2761 cagctgctgc cgctgctttg ccttatccgt ttggccgcgg tattggtcaa tctgttcgtt
     2821 tgcctggcgc agcgcatcca gcgtatcgcg tttcaggttc tgcgtattca gcacccgact
     2881 ctgctccgcc tgtgcaatgg ccgcctgaat ctgcctcatc tgaccgcgcg tactttccaa
     2941 atcgttccaa ttgctgaccg atttgctctg ctgctccaaa aacgcatgtt ccgaaataaa
     3001 attgtcggcc cgcaaacggc ggtagtctgc tgttttctgc tgctcgatcg cccccaccga
     3061 aaccagcttc tgctcctgcg ccttggccga ctgcaattcc gcctgatggc cgcgcaaagc
     3121 cgactgcaat tgcgcatcct gcgccgccca tgcctgatac tggtgctgcg ccaacacctg
     3181 cgccgattgc acatcggcat cggagagacc taaagaccgt gcttgcgcca tatcgatatg
     3241 cggcacggta cggctttcca atgccgccaa taccgcttca taacgcagtt tggacaattg
     3301 ggcagcctgc aaagcctgct ccgactgcac cacatcgctg tctgttccca cagcctccag
     3361 ttccgccagc gtttctccct gtttcacatg ctgcccgtcg cgcacatgta ccgccttaac
     3421 caccgccgtt tccagcggct ggatggtttt gctgcgcccg cccgacaccg ttttgcccga
     3481 agccgccgcc acaatatcga ttttgccgaa ccaggaccac aacaaagcca aaagcgcaaa
     3541 cgccataata aaacgcgccg cccatttcgg agcggcagag accggcgtat cggtcagttc
     3601 caaatgcgcg ggcaaaaacg cctgttcttc cgccgtgcgt ttgggcggtt ccaactggtc
     3661 gcgcaccgcc caaacattgc gccatacagt aatgtatcga gaaagaaagg atttcagggc
     3721 ggagaaaaac ataacgggta taaccttggc aatatagaaa caggaaacaa tataaatatg
     3781 taaaggaatt ttaacggaaa gcgcggcagc tgttaaggga aaggcgggaa tattgacaaa
     3841 aaatacccaa gtcgttacaa atattcatta ttttactgcg taacgcaacg ctgaagcgca
     3901 ggctgctttt gagatgcggc aaggttcggc aaaaagcagc ctgcacattt aaccacagga
     3961 acaacccatg tttaccacaa acgatttacg ccatttccta gaaggtttgg ccatcctatt
     4021 ctcaatcggc tattggggca ccatgctgct gttgctttgg tttctcgtcc gctttgccta
     4081 taaaaagccc aaacggaacc ccggcaaaat cctttcaggc agcctgaccg ccgccgctat
     4141 cctgatgctg tttgtttgga ttattcccaa acaattcggc ccgatcaaag aagaaataca
     4201 ggcacaagaa gagtgggaca gaaaatacaa agaagccgaa gccgtgttta acgaacaatg
     4261 caaaacggcg ggggaaagat ttaccagacg gcggacaatg tggaagggat tatgctgttg
     4321 aaggtagtac ctgagcgtac cgtttcggca gatgcaaaaa ccagagaccc gatgtgggac
     4381 aatgcggctt tacagaccag cgaaggcgta aattttattg ctcgtttcct aggatttttt
     4441 agcgatgggg aataccgcta tgtggatgtc ctgcaaccca accattccga tattattcgg
     4501 tattcaggta aagattttcc gataaatcaa atacttaatc atatacaccc cgcccgttat
     4561 gcggtaacgt tcgaaaacaa tgtcgattcc aagctgcgca ggcactgggt ggcaggtgcg
     4621 accatacgga ttatcgaccg ccaaactgac gaagtgattg ccaagaaaac catctatgtc
     4681 tttgaaaaag gcttggacgg cacgggtggg gcgagaatgc cgtggaagtt tgctatcttg
     4741 tgcaataaag aaagacttac ttcttcagag ccgttatcgg attttgttct tagcgtttt
//
LOCUS       DAWHXS010000123         4715 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWHXS010000123 DAWHXS010000000
VERSION     DAWHXS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.123"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            52..1560
                     /gene="nadB"
                     /locus_tag="WIE22_001822"
     CDS             52..1560
                     /gene="nadB"
                     /locus_tag="WIE22_001822"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ4127857.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKLAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLTVQIRCRPNIRVCEQQMALDIQTES
                     GAACGLTVLDCRTQATYSICARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(1738..2403)
                     /gene="pgmB"
                     /locus_tag="WIE22_001823"
     CDS             complement(1738..2403)
                     /gene="pgmB"
                     /locus_tag="WIE22_001823"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ4127858.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYLEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(2416..4674)
                     /locus_tag="WIE22_001824"
     CDS             complement(2416..4674)
                     /locus_tag="WIE22_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ4127859.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVVFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPYDEELGVFVQHDGFLDKGIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
BASE COUNT          957 a         1341 c         1350 g         1067 t
ORIGIN      
        1 aatttaatcc actataccgt ttcagacgat ctctcaaaca aggacaacac catgcaaacc
       61 gattgcgacg tattgattgc cggaaacggg ctggcggcac tgacgctcgc cctgtcgctg
      121 cctgaatcgt tccgcatcgt cattttgtgc aaaaaccggc tggacgacac tgccagccgt
      181 catgcgcaag gcgggattgc ggcggcgtgg tcgggagagg acgacatcga aaaacacgtt
      241 gccgatacct tggaggcggg cgcgggtttg tgcgacgaag ccgccgtccg caccatcctg
      301 tcgcagggca aactggcaat cgaatggctg ctggcgcagg gcgtggcgtt cgaccaaaat
      361 aataacggcc tgcacctgac gcgcgaaggc gggcacacct gccgccgaat cgcccacgtc
      421 gccgactaca cgggcgaagc cgtcatgcag agcctgactg tccaaatacg ctgccgcccg
      481 aacatccgcg tttgcgagca gcagatggcg ttggacatcc aaaccgaatc aggtgcggcg
      541 tgcggactga ccgtcctcga ctgccgaacg caagcaacct acagcatctg cgcccgccat
      601 accgtactcg caggcggcgg cttgggacag atttacgccg ccaccaccac gccgcccgaa
      661 tgcacgggcg acgccatcgc catggcgata cgcgcaggct gcgcagtcga aaacctcgaa
      721 tttatccaat tccaccccac aggcttggca agatcgtctg aaaacggccg caccttcctg
      781 atttccgaag ccgtgcgcgg cgaaggcggc atcctgacca accaagcggg cgaacggttt
      841 atgccgtatt acgaccgccg cgccgaactc gcgccgcgcg acatcgttgc ccgcgccatc
      901 gccgccgaaa tcgccaaaca aacgcaagac ttcgtctcac tcgatatcag ccatcaaccc
      961 gcagcgttcg tccgccggca tttcccgtcc atccatcggc actgtttatc ccaatgcggc
     1021 ctggatatca cgcgccaagc catccccgtc cgccccgtgc aacactacac ctgcggcggc
     1081 atccaaaccg acccctgcgg cagaacttcc ctgccgcagc tctactcctt aggcgaaacc
     1141 gcctgcaccg gcttgcacgg agccaaccgc cttgccagca actccctgct cgaatgtgtc
     1201 gttaccgcca gacttgccgc ccaaaccatc gcagacggac aagcattcca agccaaaacg
     1261 tctccaaggc cgtctgaaag cctctcctcc gaagcaggca tcttttcaga cggcatccaa
     1321 agcgcattca gccgccccgt cctgcaagcg ttcaaccaac gccatctggg catcctccgc
     1381 aacgataccg gtctgcgctg cgccatcgcc cagctgcggc tttggaaaca aaaccaaacc
     1441 gaaccgcaca ccgcgtccga atacgaaaac cgcaacctgc tcgaatgcag cctcgccgtc
     1501 gcccaagccg catacaggcg gagacagaac atcggcgcgc attttaatag tgattgttaa
     1561 ggttggctgg ttagaaaaaa aatcgttcaa ataaatttga atagcatggt taagaaattg
     1621 gcatttagtt tttagggttg cgtaagttgg gttatcaatc tgttctaaaa gcagaaaggc
     1681 cgtctgaaac actgttcaga cggcctttta cttccttgac actttatctg acgcgtttta
     1741 cctgcccgac tgtgcccaca cgttttgcag gtaggcgtag gtcagctcgg cggtatcgga
     1801 caccagcgcg atgtcgctgc ccaagtcttc ggctttgccc acgccgatag gcaaggcgcc
     1861 ggcggctttg atggcggcga cacctgcggc agcgtcttca atgccgatgc agcggcggat
     1921 gtccgcgccc acgccctcgg cggcggcgag gaagatgtcg ggggcgggtt tggaatgcgc
     1981 gacggcggca gggtcggcga cggtgtcgaa gaagtgggtc agccccatgc gttcgagcag
     2041 gaaggggccg tttttactgg cggacgcaag ggcgattttt ttgccgtttg cttttaatgc
     2101 ttccagcagc ggcaaaatgc cgagatacac gtcttcgggt ttgactgcct gtatcatctc
     2161 caagtagttg tcgtttttgc ggcgggtcag ttcggcgaac tcggcttcgc cgacggtttt
     2221 accgccgtgc gcgaggatgc gtttgagcga atcgtcgcgc gacacgcctt tgagctgctc
     2281 gttaaacttg cggtcgatgc cgatgcccag ttcttcggcg agttttttcc acgcgcggta
     2341 gtggtattcg gcggtgtcgg tgatgacacc gtcgagatcg aagaggactg cggtaaacgt
     2401 cattttgcac cctccttatt tttccaacgc ggcggcgtgg ctgccgtcga gcgtgatgtc
     2461 tttgccatac acctgcaaat cgagcggctc gcctttgagc agggtgaaga cgacgttttc
     2521 tttgccgacg gcgactttaa tcagacggcc tcggtagttg atatggaagg cgtagcctgt
     2581 ccatgcgccc ggcaggaacg gtgcgaagct gagtttaccg ccccaagttt tcatttgggc
     2641 gaaaccttgg acgatggcga gccacgagcc ggtcatagaa gtaatgtgca ggccgtcttc
     2701 ggtgtcgttg ttgtagttgt ccaagtccag gcgggcggtg cgctggtaca tttccacggc
     2761 tttttcttct ttgcccagtt cggcggcgag aatagagtgg atacaaggcg acaacgagct
     2821 ttcatgcacg gtcatcggtt cgtagaagtc gaagttgcgg cgtttttcgt cgatattgaa
     2881 acggtcgccg aagaaataga tgccttgcaa tacgtccgcc tgtttgataa agggcgaacg
     2941 caggattttg tcccacgacc atttctggtt gagcggcaaa tcgtcgggcg aaagcgcgga
     3001 cacggggcgg atgcctttgt cgaggaagcc gtcgtgttgc acgaatacgc cgagttcttc
     3061 gtcatacgga cggtacatat tcgcgctgat gtccgcccat ttttccaact cggcggcgct
     3121 cacgttcaaa tccggacgcg ggtatttcgc cagggcttcg cgggtgtagt ccaataccca
     3181 tgcggcgagg gtgttggtgt accagttgtt gttgatgttg ttttcgtatt cgttcgggcc
     3241 ggttacgccg tgaatcatgt atttgccgct gcgtttggag aagtggacgc ggtccgccca
     3301 gaagcgggac acttcgacca agacttccaa gccttctttg gcaagatagc cctcgtcgcc
     3361 ggtgtagttg gtgtagttgt agatggcgta aggaatcgcg ccgttgcggt ggatttcctc
     3421 gaaggtgatt tcccattcgt tgtggcactc gatgcccgta aacgttacca tcggatagag
     3481 tgcgcccgcc aagccctgtt cgcgcgcgtt gtgttgcgcc tgtggcagtt ggttgcggcg
     3541 gtattgcagc aggttgcggg taacttcggg ttccgccagt gcgaggtaga gcggcacggc
     3601 gtaggcttcg gtgtcccaat aggtcgcgcc gccgtatttt tctccggtaa agcctttcgg
     3661 gccgatgttc agacgcgcgt cttcgccgta gtaggtggag aacagttgga acagattgaa
     3721 acggatgcct tgctgcgctt cgtcgttgcc ttcgatgacc acgtcggcga tttcccaacg
     3781 gtgcagccag cctgctttgt gcgcgtccag caaggtttca aacacaacgc ctgcgacttt
     3841 ttccgacaag gcgcggcctg cggctttcac tgcttccaag ccctgataat cgcggctggt
     3901 ggtaacaata acccgttttt caaaggtttt gggcgtgccg ccgacttcga cctcgaaaga
     3961 attggagact tgccagtcgg tttggctgcc gccgagggct ttaaagctgc cggcgaaggt
     4021 ttgctcggcg ttgacgataa attgttccac gccgaaaggg ttggcaacgg tttgggcggc
     4081 aatgtaggag agactgtctg aaacgccttt gtccaatacc tgccagaatt tttcttcgta
     4141 gttggagtct tcgtttttca cgtcggcatc gatgatggaa tcgatgcgga cttggtgggt
     4201 tttgccgtca acggatacgg cttcccagcg gataaccgcc agctcttttt gcgcgacgga
     4261 caggaatttg cacacattga aacgcacacc gaatacggtg aacgagcggc gcaacacgcc
     4321 gtgctgcata tcaagttcga cggagaagcc ggcaacatcg tttttcgcca agtccacttc
     4381 ctgcccgtcg acaaagattt tgactttgct gaaattgagc gcgttgatgg ctttaccgaa
     4441 atatttgggg tagccgtttt tccaccagcc gacgcgggtt ttgtcgggaa accacacacc
     4501 ggcgatgtag gtacccaagt ggctgtcggc ggaataggtt tcctcaaagc tgccgcgcat
     4561 acccatatag ccgttgccca aactggtcag gctctcttgc agccgtttgt gttctttttc
     4621 cagctttgcc gaacgcagcg tccaagggct gatttccata attcttgtgt acatttatga
     4681 agctcctgtt tggattgatt tgagggaatg gtgaa
//
LOCUS       DAWHXS010000124         4635 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWHXS010000124 DAWHXS010000000
VERSION     DAWHXS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.124"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(10..219)
                     /locus_tag="WIE22_001825"
     CDS             complement(10..219)
                     /locus_tag="WIE22_001825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127860.1"
                     /translation="MPSENLSDGIFLPKQPYFLSGLQNLSETTETTLPSFPQKRESRT
                     QHGKNLSEATTIFSSSFPRRRESRT"
     gene            complement(236..1174)
                     /locus_tag="WIE22_001826"
     CDS             complement(236..1174)
                     /locus_tag="WIE22_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ4127861.1"
                     /translation="MLELNGLCKRFGNKTVADDICLTVGRSKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVAHLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1183..2058)
                     /locus_tag="WIE22_001827"
     CDS             complement(1183..2058)
                     /locus_tag="WIE22_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ4127862.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWTDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAILPETFLPENQDGEVACFEKMDIGGLLDAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2247..2323)
                     /locus_tag="WIE22_001828"
     tRNA            complement(2247..2323)
                     /locus_tag="WIE22_001828"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2287..2289),aa:Met,seq:cat)
     regulatory      2473..2572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2665..4566
                     /gene="thiC"
                     /locus_tag="WIE22_001829"
     CDS             2665..4566
                     /gene="thiC"
                     /locus_tag="WIE22_001829"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ4127863.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTRPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1108 a         1311 c         1231 g          985 t
ORIGIN      
        1 ctttagattt tacgttctag attcccgcct gcgcgggaat gacgatgaaa agattgttgt
       61 cgcttcggat aaatttttgc cgtgttgggt tctagattcc cgcttttgcg ggaatgacgg
      121 cagggtggtt tctgttgttt cggataaatt ttgcagccct gataaaaaat atggctgctt
      181 tggtaaaaaa atgccgtctg aaaggttttc agacggcatt tgtttttaag aagcatcagc
      241 ggaagcggac gatttcccgt tcttcgatat ggatgcgtac cgtatccttg cccgataccg
      301 ccccggcgtg ccgcatatcg aggttcagcc ataggatgcc gtgttccgga tggaggacgg
      361 acaggctgaa cgattcgggc aaacaggtac gggataatac gcggcactcc atgccgtctt
      421 ggtcgaaacg caccgcatgt tgcggaatat ggcggtcatc gtcggtattg ggcagcccca
      481 tcagatgggc gacctgcacg ccggcaggcg tttgaaccaa ggtttcgggc gtaccgcatt
      541 gaaggatttt cccctcgtgc atgacggcga tttcgtctgc cgccgtgcag gcctcttcgg
      601 gcgaatgcgt taccaaaacg gcagggatgc cgcccttgcg gatacgttcg gcagtcatgc
      661 ggcgcagccg gtcgcgcaaa tgcgtgtcca aactggaaaa cgattcgtcc aacagcagca
      721 gggaagggcg gacaaccaaa gcgcgcgcca gtgccaaccg ttgcttttcg cctccggaaa
      781 gtttttcagg cttgcggtgc gcctcgtttt ccagtccgac ttcggcaagt gccgccatgg
      841 cgaggctttc ggcttcggct ttcggcattt tttgcatttt caaaccgaat gccgcatttt
      901 ccagtgcact catatgggga aacagcgcgt aatcttgaaa catcagcgaa atacggcgtt
      961 tttcgggcgg catacgggta atgttttccc cgttcagccg aatttcgccg ccgtccggac
     1021 ggataatccc cgcaatcata ttcagcaggg tggattttcc gcagcccgac cgtcccaaaa
     1081 cggcaagtat tttgctgcgg ccgacagtca ggcagatgtc gtcggcgacg gttttattgc
     1141 cgaagcgttt gcagagtccg ttcagttcaa gcatggcgta tcctataaac gtatgccgtc
     1201 cagccactcg gacagcggat gggcggcatc aatcagaccg taacggcaaa acgcgtccag
     1261 cgtaaccagt tgcgcgtcgt gcatcatgtt ttccgacaac atggcatcca acagaccgcc
     1321 gatgtccatc ttttcaaaac acgcaacctc gccgtcctga ttttcaggca ggaaggtttc
     1381 gggcaggatg gcatcgaata catacaggat ttcattgtgc acaccccggc tgacggggcg
     1441 caggctgtgc agccgagata ccggacggat aaacgggaac agcgttttat ccaaaccggc
     1501 ttcttcgctg ctttcgcgac acacggtttc agacggcaat tcaccgctgg aaacaccgcc
     1561 ggcggcagta ttgtcgagtt tgtcgggatc gactgctttg tgcggactgc gcctgcctat
     1621 ccagaaatgc catcggccgt ccgattcggt cagaccgttg agatggacgg cgcggctgag
     1681 tagtccgaac gggcggaaag cggcgcgttc gagcgtgaac aaggggttgc cgccgccgtc
     1741 ggtcaggtcg aaacactcgt tgcgccagcc gtgaagcagc cccgccttgt tccatgtgcg
     1801 ggcgaggtgc tgcaagcgtc cgcccatatc cgtccagccg tccgcattca gaaaaatgcc
     1861 gtctgaagac tccgagcagc ctgcctccca gtcttttttg acgcgctccg cccattccgg
     1921 cgacagattg cccaaaggca gacggttcag atacagcgtt ttccagcaac tttttgcacc
     1981 gtaacttgct tttgcccact cgaacagggc atcaaggtct tgtttgctga cggattcggt
     2041 aaaacggacg gtcggcatag tcggctttct tgaacataat gcggaagatt gtagccaaac
     2101 cgcctgaatt tgcaaccgca aacagacggc gggctgccgt tccgatattg cggcaggaag
     2161 gaaaatccgg caaaaaacgg aggcggcagg aaacaggaaa caggcaaaac aaaacccctc
     2221 ggcattgaac cgaggggttt aatatttggt tgcgggagca ggattcgaac ctacgacctt
     2281 cgggttatga gcccgacgag ctaccatgct gctccatccc gcgtcagaag atggaactat
     2341 acggcagatt ttttatgttg tcaaacatta tttccgcgaa aaatcataaa attttgccgt
     2401 ccggcggttt atgcctgatt tgaaatatca ttttctttac aaaagttcat gttcgtgatt
     2461 taattttggt taacattgaa acaggggtgc tgcatgatgt tcaggcggct gagaaatacc
     2521 ctttacaccc gatctggata atacctgcgt ggggagtttt cacggattct gcctttcaga
     2581 cggcattttt tgatactcgg gccgtttgaa aaagcaaagt gctcctgttt ctttattcta
     2641 aacgagaaaa caggagcatt ttttatgact acgccaaaaa aaactgccaa aacttccggc
     2701 aacgaagcgc gcgaacttgc cgacttgagc gaagacatcg gcatccgctt caaatatccg
     2761 aactcggagc gcgtgtatct gcaaggcagc cgcgacgaca tccgcgtgcc tttgcgcgaa
     2821 atccgtcagg acgacaccta cacggcgcaa ggcacggaag ccaacccgcc gattcccgtc
     2881 tatgacacca gcggcgtgta cggcgatccg gcggcgcaca tcgacctgaa acaaggtctg
     2941 ccgcacatcc gcacggcgtg gctggacgaa cgcggtgata ccgaaatcct gcccaagctt
     3001 tccagcgaat acggcatcga acgcgcacac gatccgaaaa ccgcccatct gcgcttcaac
     3061 caaatcaccc gcccgcgccg cgccaaagcc ggccgcaacg taacccagct ccattacgcc
     3121 cggcaaggca ttatcacgcc ggaaatggaa tttgtcgcca tacgcgaacg tttgaaatta
     3181 gacgaattgt cccaaaaacc ggaatacgcc aaactcttga agcaacacgc ggggcaaagt
     3241 ttcggcgcga acatcccgac ccgtcccgat caaatcacac ccgaattcgt gcgccgggaa
     3301 atcgccgccg gacgtgcgat tattcccgcc aacatcaacc atcccgaact cgaaccgatg
     3361 attatcggcc gcaacttccg cgtcaaaatc aacggcaact tgggcaattc cgccgtcact
     3421 tccagcctga ccgaagaagt cgaaaaaatg gtgtggtcgc tgcgttgggg cgcggacaca
     3481 attatggact tatccacagg cgcgcacatc catgaaacgc gcgaatggat tatccgcaac
     3541 gcgcccgtcc ccatcggcac cgtgcctatt tatcaggctt tggaaaaaac cggcggcatt
     3601 gccgaagatt tgacttggga tttgttccgc gacaccttaa tcgagcaggc ggaacaaggc
     3661 gtggactatt tcaccataca cgcgggcgtg ttgctgcgtt atgtaccgat gaccgccaac
     3721 cgcctcaccg gcatcgtctc gcgcggcggt tcgattatgg cgaaatggtg tttggcacat
     3781 catcgggaaa acttcctcta cacgcatttc gacgaaatct gcgaaatcat gaaagcgtat
     3841 gatgtgtcgt tcagcctcgg cgacggcctg cgccccggct gcattgccga tgccaacgac
     3901 gaatcccaat tcgccgagct gcacaccttg ggcgaattga ccgataaagc gtggaaacat
     3961 gacgtacaag tcatgatcga aggccccggc catgtgccgc tgcaacgcgt caaagaaaac
     4021 atgaccgaag agctgcaaca ctgctttgaa gcaccttttt acacactcgg cccgctcgtt
     4081 accgacatcg cacccggcta cgaccacatc acctcgggca taggcgcggc caatatcggc
     4141 tggtacggca cggcgatgct ttgttacgtt acccccaaag aacatttggg gctgcccgac
     4201 aaagaagacg tgcgcaccgg catcatcacc tacaaactcg ccgcccacgc cgccgatctc
     4261 gccaaaggct ggccgggcgc acaattacgt gacaacgccc tgagcaaggc gcgtttcgag
     4321 ttccgttggc gcgaccaatt tcgcttaagc cttgaccctg aacgtgccga gagcttccac
     4381 gacgaaaccc tgcctgccga aggcgcgaaa atcgcccact tctgctcaat gtgcggcccc
     4441 aaattctgct cgatgaaaat cacgcaggaa gtgcgcgact acgccgacaa gcaaaaagcc
     4501 caacggcagg gcatggagga aaaagcggtc gagttcgtca aaaaaggcgc gaagatttac
     4561 agttgaaacg tcaagcaaaa aatgccgtct gaaacccgga aaaaaggctt cagacggcat
     4621 tctttcgctt gtgaa
//
LOCUS       DAWHXS010000125         4621 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWHXS010000125 DAWHXS010000000
VERSION     DAWHXS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.125"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(418..663)
                     /locus_tag="WIE22_001830"
     CDS             complement(418..663)
                     /locus_tag="WIE22_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127864.1"
                     /translation="MLKSIELNSHVRNRLAAYLKGRGMDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            780..1205
                     /locus_tag="WIE22_001831"
     CDS             780..1205
                     /locus_tag="WIE22_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ4127865.1"
                     /translation="MYSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYVR
                     LSDMAVRLYRFMSPLGFGAVVFGTAIPFAAGWWGSGWVHVKLCLGLILLAYQLYCGVL
                     LRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1217..1693
                     /locus_tag="WIE22_001832"
     CDS             1217..1693
                     /locus_tag="WIE22_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ4127866.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2043..2531)
                     /locus_tag="WIE22_001833"
     CDS             complement(2043..2531)
                     /locus_tag="WIE22_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ4127867.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2599..3654)
                     /gene="aroG"
                     /locus_tag="WIE22_001834"
     CDS             complement(2599..3654)
                     /gene="aroG"
                     /locus_tag="WIE22_001834"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ4127868.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            4006..4211
                     /locus_tag="WIE22_001835"
                     /pseudo
     CDS             4006..4211
                     /locus_tag="WIE22_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4300..4578
                     /locus_tag="WIE22_001836"
     CDS             4300..4578
                     /locus_tag="WIE22_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127869.1"
                     /translation="MRGKTKVMTTQMCASCDVSKSAKGWSVGIGLNVGKQLTDSVGLE
                     FDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
BASE COUNT         1152 a         1189 c         1057 g         1223 t
ORIGIN      
        1 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
       61 tatactacat cgcaatgaaa aatcaggaaa agcaaaaaac cctccgccgt cattcccgcg
      121 aaagcgggaa tctagaattt taatgcggca aaaatttatc ggaaaaaacc gaagtttaaa
      181 gacctagatt cccgcctgcg cgggaatgac ggtttagaaa tcacccgaaa cgagaaaaag
      241 aaccgaaacc gaacaagccg gattaccgcc tgcgcgggaa tgacggctca aaagttacga
      301 aacgaaaaat aaccgaaacc agacaagtcg gattcccgcg cgggcgggaa tgacgataag
      361 cgtaatttca atctgccata ttgtcttttc acttgcctga aaacagattt cagccgccta
      421 tccctgcatc cggcggctga tgtagtcggc aatcaaatcc cgcttttccc aaaaaaattt
      481 ctgcattttt tgttcggaat acagtttgcg gaccagagtg ggcagaccgc tgtggacgat
      541 ggcggcgatt tctttgttgc cttcttcttc ctgcattgcc gtctgaaaat ccatgcccct
      601 gcctttcaga tatgctgcaa ggcggttgcg gacatgcgaa ttgagttcga tagatttgag
      661 catttttcct ccgttttcga taacgattat atttaatccg tctgtatgcc ttattttatc
      721 atgtttcacg atcggtttgc ctgtacaata ccgcttttca gtatgatgtg ctggcattta
      781 tgtattcttg gttcaagctg tttcacttgt tttttgtcat ttcgtggttt gcagggctgt
      841 tttacctgcc gaggattttc gtcaatatgg cgatgattga tgcgccgcgc ggcaatcccg
      901 agtatgtgcg cctgtcggat atggcggtgc ggctgtaccg ttttatgtcg ccgttgggct
      961 tcggcgcggt cgtgttcggc acggcgatac cgtttgccgc cggctggtgg ggcagcggct
     1021 gggtacacgt caaactgtgt ttgggcttga tactcttggc ttaccagttg tattgcggcg
     1081 tgctgctgcg ccgttttcag gattacagca atgctttttc acaccgctgg taccgcgtgt
     1141 tcaacgaaat ccccgtgctg ctgatggttg ccgcgctgta tctggtcgtg ttcaaaccgt
     1201 tttgacggag catcttatgc cgatagaaat cgaacgccgt tttttgattg gaaacgacaa
     1261 ctggcggcaa tacgccgacg aaccgctgct gttgaggcag ggttatctgt ctgttgaaaa
     1321 agaacgcacc gtccgtgtcc gcattgccgg acaacgggcg tggctgacgc tgaaaggcta
     1381 tatttcggaa atcagccgca gtgagtttga atacgaaatc ccgcttgccg atgcggaaaa
     1441 gatgatggaa acgatgtgcc cgtttaaaat ggaaaaatgg cgttatccgg tcagatgggg
     1501 cggcagcctg tttgaaatcg atgttttcct tggcgaaaac gcgcctttgg tcgtcgcgga
     1561 aatcgagttg cccgacgaga atgccgattt tgaccgtccg gactggctgg ggcgcgaaat
     1621 cactgccgac ggtatgttta ccaatgctta tttgagcagg catccgttct caagctggaa
     1681 aaatgccgtc tgaaacggct tcagacggca ttctccgaca accatcgcaa atccaaagac
     1741 agcctctata tccttatttc tgctttcttt gttccattat tcccaataaa tccccataac
     1801 ctaaaatctc gtcattcccg cgcaggcggg aatccggttc tttaaacttc ggttttttcc
     1861 gataaattgc tttagctttt cgtttccgga ttcccgcttt tgcgggaatg acgatggaag
     1921 attgttgtta tttcggataa attaccctaa ctttaaaatc ccggattccc atcttcgggg
     1981 gaataacaat attttctatt tcagcccatc atttgcgggg gattttatag agagtttata
     2041 tttcaatatc tcaaataatg cacaaaagca tagcgcgtgc ctttgctgct gacgcggcgt
     2101 tccgtccgcc ctgcctcttt ccaatgcgtc cggtctattt cggggaaaaa cgcatctcct
     2161 tccacagaca aatccacttc ggttatccgc aaatcggtcg ccaatggcat cgcttgtccg
     2221 tatatctgcg cgccgcccat aatgacggct tcttccgcgc ctgcgcacaa tgccaatgcc
     2281 gcctccaaac ttgccgccgt ttccgcgcct gccgcgcaat aatccgcctg ccggctgatg
     2341 acgatgttcc tccgtccggg caggggtttg acgggcaggg attcccacgt tttccgcccc
     2401 ataatgacgg gtttgcccaa ggtataggct ttgaaaaatg cgaaatcttc ggggatgtgc
     2461 caaggcatag cattgcccgc cccgatgcac aggttttccg cacacgccgc aattagggtt
     2521 attttgagca tatttcctcc acaaaaatgc cgtctgaacg cttcagacgg cattttatga
     2581 cattccgtgt caaaaatctc aaccggcgcg cgccatacgt tttttgtttg cacctgccaa
     2641 caatgccaac agttcttcag tcgcgcccca accgatacac gcatcggtaa tgcttttgcc
     2701 gtacacttcc ggcttatcct gtctgccttc gaccaaatgg ctttccacca tcacgcccat
     2761 aatattgctc ccgtcctgtt ccaattgggc agcaatatct tgtgccactt ccatctgccg
     2821 ggtgtaatcc ttgcggctgt tggcgtggct gcaatctatc atcagcttat cggttacccc
     2881 tgccgcacgc agttgttccg ccgcctcgct gacgtgtccc gcatcataat tcggctcttt
     2941 tccgccgcgc aaaatgacat gacagtcggg attgccgccg gtatggacaa tggcggaatg
     3001 tccggccttg gttacagaca agaaatgatg cgaatggctc gccgcaccga ttgcgtcgat
     3061 ggcaatcttc aaattgccgt ccgtaccgtt tttaaagccg acggggcagg acagcccgct
     3121 tgccaattcg cggtgaactt ggctttcggt cgtccgcgca ccgattgccc cccaagaaat
     3181 caagtccgca taatattgcg gcgtaatcat atccaaaaac tcggtagagg caggcatacc
     3241 catattgttc agcgacaaca acaggctgcg cgcctgacgc aaaccgaaat tgatatcgaa
     3301 cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa cccaccgtcg tcctcggctt
     3361 ctcgaaataa acgcgcatca caatcaaaag ctcgttttca tactgcttgc ggagtttcaa
     3421 caaacgctcc gcatattcca acgccgcttt cggatcgtga atcgaacacg gcccgataat
     3481 aaccaacagc cgcttgtccc tgccgtgaac caaatcggaa atttcctgac gggtgcggtg
     3541 aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa agatgggcaa tcggcggcaa
     3601 caactcttta acttctttaa tcttaatatc gtctgtgggg taatggtgtg tcatcttgca
     3661 tctttcctga tggtttttta tacgcggctt aggttacatc gtttaccttg cacaaacaag
     3721 tgcaacatat tactacaaag ctattttatt tcaaaataac tgatatatct tttttcatag
     3781 attttaaatt tcatattttt aatattttag gaattttatt ccaaagaaaa agaaacagat
     3841 tgataaaaac aatacccgcc attcattcgg acaaaaccgc acggaacatc cgttttccca
     3901 tttccacgac aatccggtac agaacacccc gctgccgatt ccgcctgcac atccggcaaa
     3961 atttcataca caaggaaaat gttgaaaaca aaaagaactc attgcttgaa aaaagattta
     4021 atgggaatgc gggtatttcc cacacttttt ccatttattt atcctcatta taggaagaaa
     4081 aacatgaaaa aaccctgctt gcactctcac ttgcagccgt gtccgccgtt acggtagccg
     4141 atattcccga gtctaacggc aaaggcaaag ccatcatcgg cgataaggaa aaacaccgtt
     4201 accaagcatg aaaaccttgc tttgaaagtc catggcgaaa tccgccaaga tttgggggac
     4261 ggatgatccc taaccccttg ggcggatgca tatgcagatt tgcgcggcaa aaccaaagtg
     4321 atgacgaccc agatgtgtgc ttcctgcgat gtcagcaaaa gcgccaaagg ttggagtgtc
     4381 ggtatcggtc tgaatgtagg caaacagttg accgacagcg tcggtctcga gtttgatcca
     4441 tactaccgtc acaaaacaat ctgcaaaccc cgtgagattg ttttggacgg cgacaaaacc
     4501 aaaatgggcc gctccaaatc caacgagtac ggcttccgcg tagccgcaac gttctatagt
     4561 caattaaaat caaaatagga cagtagtgca tcgtcaaatc gggcgtaatc agacaaaacg
     4621 g
//
LOCUS       DAWHXS010000126         4612 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWHXS010000126 DAWHXS010000000
VERSION     DAWHXS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.126"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1556
                     /locus_tag="WIE22_001837"
     CDS             <1..1556
                     /locus_tag="WIE22_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ4127870.1"
                     /translation="YYNASIADGYTLRLIREAISSQYKAQLQAALAQLEVEKGSFDRK
                     EIYAHPHFVRPMLDYILGDFAKFRKTNQDESLGAMVVCDSAEQARQLFEHFQTASDHN
                     FTAALILHDVGTKDERDQWVKDFKAGKTDILFVYNMLLTGFDAPRLKKLYLGRLIKAH
                     NLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAYWDELSNELGDEIGSYSQLFK
                     TAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKKQLLALKKALQTSKELYNALR
                     LQGSHEFLVHLDFDKLNLLYRETAARLDTLNLAEKLQQGDTAHLLNEALEDVYFQFVK
                     IGEAELKLADDLKDIMRKVREGLAGNFDQDDPEYISLREELERIFKKKNLAEVGQEEM
                     QANIATLQIVYAKIKELNRQNDLLRHKYGGDAKYARTHKRLMENAAFYGDKLKVFNAL
                     NGVKADADQKVLDMEQILDNQNYFEKQMQGIVLKRFRTEQQFPVQPQDIQAINRLLVR
                     EYLKESGRM"
     gene            1683..2876
                     /locus_tag="WIE22_001838"
     CDS             1683..2876
                     /locus_tag="WIE22_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ4127871.1"
                     /translation="MKRQITIGEIAEINANSLTQKDMFQEIMYLDTGNITRNEIDNIQ
                     VLNVMMDKIPSRAKRKVKDKTIIYSTVRPNQEHYGFLENPGDNFIISTGFSTIDVYDE
                     NTDEKFIYYLLTQKHITDYLHTIGENSVSSYPSINPDDIANLKFAVPDLKTQQSIAAV
                     LSALDKKIALNKQINARLEDMAKTLYDYWFVQFDFPDANGKPYKSSGGEIVFDETLKR
                     EIPKGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNP
                     QDAHIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYA
                     RHFKFLKEFKIILPSKDISQKYNEIANTFFVKVRNNIKQNHQLTQLRDFLLPMLMNGQ
                     VSVAE"
     gene            2924..4525
                     /locus_tag="WIE22_001839"
     CDS             2924..4525
                     /locus_tag="WIE22_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ4127872.1"
                     /translation="MTEQHFTEQTKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEPIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCHALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPKEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDTSGRLKKFDFIVSNPPFKLDFSDFREQLEGEENRERFFAGIPKIKAKD
                     TDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSSIDKKIREYLVENKMLAGMVS
                     MPSNIFATTGTNVSILFIDKANKDNVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISADEFAQKMAGFSADL
                     DKLFAESAKLEKEIKDRLAMLEFNS"
BASE COUNT         1505 a         1020 c          961 g         1126 t
ORIGIN      
        1 actattacaa cgcctccatc gccgacggct acaccctgcg cctgattcgc gaagccatca
       61 gcagccaata caaagcccaa ttgcaggctg cactggcgca gcttgaagtc gaaaaaggca
      121 gctttgaccg caaagaaatc tacgcccatc cgcatttcgt ccgcccgatg ctcgattaca
      181 tcttgggcga cttcgccaaa ttcagaaaaa ccaaccaaga cgagagcctc ggcgccatgg
      241 tcgtttgcga tagcgcagaa caggcgcgcc agctttttga gcattttcag acggcctcag
      301 accataattt caccgccgca ctgatattgc acgacgtcgg caccaaagac gaacgcgacc
      361 aatgggtcaa agatttcaaa gcaggcaaaa ccgacatcct gtttgtgtac aacatgcttt
      421 tgaccggctt tgacgcgccg cgcctgaaaa agctctattt gggcagactc atcaaagccc
      481 ataacctttt acaaaccctg acccgcgtca accgcaccta caaatcttac cgttacggct
      541 atgttgtcga ttttgccgac atcgaacgcg aatttgacaa aaccaaccgc gcctattggg
      601 acgaactttc taacgaattg ggcgacgaaa tcggcagtta cagccagcta ttcaaaaccg
      661 ccgaagaaat cgaacaggaa attgcagaca ttaaaaacgc cctgtttgat ttcgataccg
      721 aaaacgccga agaattttgc agccaaatta gccaaataga agacaaaaaa cagcttctcg
      781 ccctaaaaaa agccctgcaa acttccaaag agctatacaa cgctctgcgc ctgcaaggca
      841 gccacgaatt tcttgtgcat ctggattttg acaagctcaa ccttctgtac cgcgaaactg
      901 ccgcccgtct ggatacctta aaccttgccg aaaaattaca gcaaggcgac accgcccatt
      961 tgctcaatga agcgctggaa gacgtttact ttcaattcgt caaaatcggc gaggcggaac
     1021 tcaaacttgc cgacgattta aaagacatca tgcgcaaagt ccgtgaaggg ctggcgggca
     1081 acttcgacca agacgacccc gaatacatca gcctgcgcga agagctggaa cgcatcttca
     1141 agaaaaagaa cctcgccgaa gtcgggcaag aagaaatgca ggcaaacatc gccacactcc
     1201 aaatcgtcta tgccaaaatc aaagagctga accgccaaaa cgaccttttg cggcacaaat
     1261 acggcggcga cgctaagtat gcccgcaccc acaagcggct gatggaaaac gccgccttct
     1321 acggcgacaa actcaaagtt tttaacgctc taaacggcgt caaagccgat gccgaccaaa
     1381 aagtgctgga tatggagcag attttggaca atcaaaatta ctttgaaaaa caaatgcagg
     1441 gcatcgtatt aaaacgcttt agaacggagc aacaatttcc tgttcaaccc caagacatcc
     1501 aagccatcaa ccgcctgttg gtgcgggaat atttaaaaga aagcgggcgg atgtgagaaa
     1561 gcggtttttt aggtagcctt tatgggcgta ttgcatacgt tcgaagtgga caataaaagt
     1621 gcggttggat tttgaaattt ttttcagacg gcctttgaag agtttcgaga gattaagtta
     1681 agatgaaacg acaaattaca attggtgaaa tcgcagaaat aaatgccaac tccctcactc
     1741 aaaaagacat gtttcaagaa atcatgtatt tggatactgg caatattact agaaatgaaa
     1801 tagataatat ccaagttttg aatgtcatga tggataaaat acctagccgt gcaaaaagaa
     1861 aagtaaaaga taagactatt atctattcta cagttcgccc aaatcaggag cattacggtt
     1921 ttttggaaaa tcctggtgat aatttcatta tatcaacagg gtttagtaca attgatgttt
     1981 atgatgagaa tacggatgaa aaatttattt actatttatt gactcaaaag catatcactg
     2041 attatttaca tacaattggc gagaacagtg tttcatcata tccatcgata aatcctgatg
     2101 atattgcaaa tctaaaattt gcagttcctg acctcaaaac ccaacaatcc atcgccgccg
     2161 tcctatccgc tttggacaaa aaaatcgccc tgaataaaca aatcaatgcg cgcctggaag
     2221 atatggcgaa aaccctgtac gactattggt tcgtgcagtt cgacttccct gacgccaacg
     2281 gcaagcccta caaatcctca ggcggcgaaa ttgtgtttga tgaaaccttg aaacgggaaa
     2341 ttccgaaggg gtggggaagt atagaattac aatcttgctt agcaaaaatc ccaaatacta
     2401 caaaaatatt aaataaagat attaaggatt ttggaaaata tcctgttgtt gaccaaagtc
     2461 aagattttat ttgtggattt accaatgatg aaaaatctat attaaacccg caagatgctc
     2521 atattatttt tggtgatcat acaagaattg ttaagttagt taattttcaa tatgcgcggg
     2581 gagcagatgg tacacaagta atattaagca ataatgaacg aatgccaaac tatttgtttt
     2641 accaaatcat aaatcaaata gatttatcaa gttatggata tgctagacat ttcaaattct
     2701 taaaagaatt taaaataatt ttacctagta aagatatttc tcaaaaatat aatgaaatag
     2761 caaatacttt ctttgttaaa gttcgaaata atataaaaca aaaccaccaa ctaacccaac
     2821 tccgcgattt cctacttccc atgctgatga acgggcaggt ttctgtggcg gaataagcca
     2881 aattcaagaa tcagacctac aaaataaaac aaacgagaaa aaaatgaccg aacaacactt
     2941 taccgaacaa accaaatccc taatcgacag cttaaaaacc atttgcgcca actatggttt
     3001 gggcaatgac ggcaacgagt tcaaaatcat cagtcaggca tttttgtata aattcctaaa
     3061 cgacaaatac gatttcgaag ttaaacaaat tcgcaaagaa aatcccgacg agccaatcga
     3121 attcatcaat atggatattg agggaaaaac agccgttttg aagcctgaac attccatcaa
     3181 gtatttatca gagcgacaaa acggcgcgga ctttgccaaa ctctttgacg atacgctgac
     3241 cgatattgcc gcacataatg cggaactgtt ttcggtaaaa accgaaggcg gggcgaaaat
     3301 cgtgttgttc gagcgtatca gtcaatatat tacggatgaa ggtcgccgcg acgatttttg
     3361 ccacgcgctg atttccaaac tggcgggttt cagcttcgag gcgatttttg cgcaaaaatt
     3421 tgattttttc gccacgattt ttgagtacct gattaaagac tacaacagta attcgggcgg
     3481 caaatatgcc gaatactaca cgccgcacgc agtagcgcgg attatggcgg atattttggt
     3541 gccaaaggag gtacgtgggc aaatccgcag cgtggacgtt tacgatccgt cagccggttc
     3601 aggaacgctg ctgatgaacg tggcgcacgc catcggtgaa gacaaatgta tgatttatac
     3661 gcaggatatt tcgcaaaaat cgtccaatct tttgcggctg aatctgattt tgaacaattt
     3721 ggtgcattct ttgaataatg tggttcaggg taatacgatt ttgtcgcccg cccataaaga
     3781 cacttcaggt cgtctgaaaa aatttgattt cattgtttcc aatccaccgt ttaaattgga
     3841 tttcagcgat tttcgcgagc aattggaagg cgaggaaaac cgcgaacgct tttttgccgg
     3901 tatcccaaaa atcaaggcaa aagatacgga taagatggaa atttaccagc tttttatcca
     3961 gcatatcttg tttagcctga aagaaaacgg caaagcggcg attgtgttgc cgacgggctt
     4021 tatcaccgcc caatcgagta tcgacaaaaa aatccgcgaa tatctggtag aaaacaaaat
     4081 gctcgccggc atggtttcca tgccttccaa tattttcgcc accacgggaa ccaacgtttc
     4141 catcctgttt atcgataaag ccaataaaga caacgtggta ttaatcgacg catcgggctt
     4201 gggcgaaaaa atcaaagacg gcaaaaacca aaaaaccgta ctctcccgcg aagaagaaca
     4261 aaaaatttgc aatactttca cgcacaaaca ggcagtggaa gatttcagcg tggtggtcgg
     4321 ctacgatgaa atcaaagcca agaattacag cctgtcggcg gggcagtatt tcgaggtaaa
     4381 gattgattat gtggatattt ccgccgacga atttgcgcaa aaaatggcgg gattttcagc
     4441 ggatttggat aaacttttcg ccgaatctgc caaattggaa aaggagatta aggataggct
     4501 ggcaatgttg gaattcaatt cgtaaatacc gatgaatgaa accggtcaaa cttaaaatgc
     4561 cgtctgaaag gctttcagac ggcattttgt ttggtttgga aaatacttat gc
//
LOCUS       DAWHXS010000127         4503 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWHXS010000127 DAWHXS010000000
VERSION     DAWHXS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.127"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(145..1287)
                     /locus_tag="WIE22_001840"
     CDS             complement(145..1287)
                     /locus_tag="WIE22_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4127873.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1591..1854
                     /gene="rpsT"
                     /locus_tag="WIE22_001841"
     CDS             1591..1854
                     /gene="rpsT"
                     /locus_tag="WIE22_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ4127874.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            2014..3162
                     /locus_tag="WIE22_001842"
     CDS             2014..3162
                     /locus_tag="WIE22_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ4127875.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            3305..4006
                     /locus_tag="WIE22_001843"
     CDS             3305..4006
                     /locus_tag="WIE22_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ4127876.1"
                     /translation="MTEPEAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            4064..>4503
                     /locus_tag="WIE22_001844"
     CDS             4064..>4503
                     /locus_tag="WIE22_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ4127877.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVA"
BASE COUNT         1080 a         1126 c         1219 g         1078 t
ORIGIN      
        1 gttggaatat agggttaaaa tagggctctt ttgggcttat tcaaattagg gacacgccct
       61 aatccactat ataaatattt ctatttcaat ccaatataaa atgccgtccg aacgatgttc
      121 ggacggcatt tttaatgctt caaatcagtt ggtgccgact ttgattttct gccacaggct
      181 gacagacagt tttttcgcat ccgtactcat ttgcggcatc acaaaaccgt ctttcatatc
      241 ctgctcgttc gggaagatgg aacgggtgtt caccagctcg gcaggcattt tttcgcgcgc
      301 cggtttgctg gcgggggcga aggttacggc gatgccgttt ttcgccgcga tttcggggtc
      361 gagcgtgtag ttgatgtatt tgtgggcatt ggcgacgttt ttcgcatcgg cgggaatcag
      421 ccaagactca atccagaagc ccatgccttt cggattcagc acttcgatgc cgacgttgtt
      481 tttcacttct tcggaacgtg ctttcgccaa gttcaaatcg ccgccgttgc ctgccgccag
      541 gcagatgtcg ccgcgcgcca gctcgtcgat gatggacggg ctgaaacgtt tgacatccgg
      601 acggatagac ttcaacactt ccgccgccgc cttcaaatct tcaggattcg aacctttggg
      661 gtctttgccc aagtagttca gcaaaatcgg gaacatttcg ctcggggtgt cccacagggc
      721 gatgccgcag gatttcagct tgtgggtgta ttcgggtttg aacagcaaat cccagccgtt
      781 ttcgggcagc ttgccgccca aaagctcttt gcccttcgcc gtaatcgcaa tcgtgttcac
      841 gccggagaaa taggggacgg catactggtt gcccgggtcg gcggtttcca gcattttcaa
      901 gagttcggga tcgatgtttt tatagttggg aatcaggtct ttgttgactt tttgatacgc
      961 gcccgcctca atttggcgcg gcaggaaggc gatgcccggc acaaccaaat cgtaaccgga
     1021 tttgccggtc agcattttgg cttccagcgt ttcattgttt tcgtacaagt cgtaagtcag
     1081 cttcagattg ttggcttttt taaagtcttc gaccgtactc tcgtcaacat agttcgacca
     1141 gttgtagatg ttcagagtat cggtggcagc ggcttcggca ttggcagcag acgcagcgtc
     1201 tgcttgaggt tgcacggcgt ttttttcgct gccgccgcag gctgccagag acagcgcggc
     1261 caaaacggct aatacggatt ttttcatacg ggcagattcc tgatgaaaga ggttggaaaa
     1321 aaaagaaaac cccgcgcccc ataaacaccc cggcgcaagg tttgggcatt gtaaagtaaa
     1381 tttgtgcaaa ctcaaagtga tattggactg atttccctaa aaaattaccc tgttttcaaa
     1441 agagggggaa aacgtccgca cgattttgcc gtttttttgc gctgtcaggg tgtccgacgg
     1501 gcggatagag agaaaaggct tgcatataat gtaaaccccc tttaaaattg cgcgtttaca
     1561 gaatttattt ttcttccagg agattccaat atggcaaaca gcgcacaagc acgcaaacgt
     1621 gcccgccagt ccgtcaaaca acgcgcccac aacgctagcc tgcgtaccgc attccgcact
     1681 gcagtgaaaa aagtattgaa agcagtcgaa gcaggcgata aagctgccgc acaagcggtt
     1741 taccaagagt ccgtcaaagt catcgaccgc atcgccgaca agggcgtgtt ccacaaaaac
     1801 aaagcggcac gccacaaaag ccgtctgtct gcaaaagtaa aagcactggc ttgatttttg
     1861 caaaaccgcc aaggcggttg atacgcgata agcggaaaac cctgaagcct gacggtttcg
     1921 gggttttctg tattgcgggg gcaaaatccc gaaatggcgg aaagggtgcg gtttttatcc
     1981 gaatccgcta taaaatgccg tttgaaaacc aatatgccga caatgggggc ggagatgaat
     2041 acacggaata tgcgctatat tcttttgaca ggactgttgc cgatggcatc cgcttttgga
     2101 gagaccgcgc tgcaatgcgc cgctttgacg gacaatgtta cgcgtttggc gtgttacgac
     2161 aggatttttg cggcacagct tccgtcttcg gcagggcagg aagggcagga gtcgaaagcc
     2221 gtactcaatc tgacggaaac cgtccgcagc agcctggata agggcgaggc ggtcattgtt
     2281 gttgaaaaag gcggggatgc gcttcctgcc gacagtgcgg gcgaaaccgc cgacatctat
     2341 acgcctttga gcctgatgta cgacttggac aaaaacgatt tgcgcgggct gttgggcgta
     2401 cgcgaacaca atccgatgta ccttatgccg ctctggtaca acaattcgcc caactatgcc
     2461 ccgggttcgc cgacgcgcgg tacgactgta caggaaaaat tcggacagca gaaacgtgcg
     2521 gaaaccaaat tgcaggtttc gttcaaaagc aaaattgccg aagatttgtt taaaacccgc
     2581 gcggatctgt ggttcggcta cacccaaaga tccgattggc agatttacaa ccaaggcagg
     2641 aaatccgcgc cgttccgcaa tacggattac aaacctgaaa ttttcctgac ccagcctgtg
     2701 aaggcggatt tgccgttcgg cggcaggctg cgtatgctcg gtgcgggttt tgtccaccag
     2761 tccaacggac agagccgtcc cgaatcgcgt tcgtggaaca ggatttacgc catggcaggc
     2821 atggaatggg gcaaattgac ggtgattccg cgcgtgtggg tgcgtgcgtt cgatcagagc
     2881 ggcgataaaa acgacaatcc cgatattgcc gactatatgg ggtatggcga cgtgaagctg
     2941 cagtaccgcc tgaacgacag gcagaatgtg tattccgtat tgcgctacaa ccccaaaacg
     3001 ggctacggcg cgattgaagc cgcctacacg tttccgatta agggcaaact caaaggcgtg
     3061 gtacgcggat tccacggtta cggcgagagc ctgatcgact acaaccacaa gcagaacggt
     3121 atcggtatcg ggttgatgtt caacgacttg gacggcatct gaaccgcgtg ttcagacggt
     3181 atatcaagtt gccgtgccgt ctgaagccgc tggcggtttg gcggggcggg ataaaaattt
     3241 cgtttgaatc cgggcaaacg cgtataatgt gcggtttgta cggactttgt cggaatcaag
     3301 caagatgacg gaacctgagg ccgaaggcgg taaagctgcc aaggcgttaa aaaaatatct
     3361 gattacgggc attttggtct ggctgccgat tgcggtaacg gtttgggtgg tttcctatat
     3421 cgtttccgcg tccgatcagc tcgtcaacct gctgccgaag caatggcggc cgcaatatgt
     3481 tttgggcttc aatatcccgg ggctgggcgt tatcgttgcc attgctgtat tgtttgtaac
     3541 cggattgttt gccgcaaacg tattgggccg gcagattctt gccgcgtggg acagcctgtt
     3601 ggggcggatt ccagttgtga agtccatcta ttcgagtgtg aaaaaagtat ccgaatcgct
     3661 gctgtccgac agcagccgtt cgtttaaaac accagtactc gtgccgtttc cccaatcggg
     3721 tatttggaca atcgcattcg tgtccggtca ggtgtcgaat gcggttaagg ccgcattgcc
     3781 gaaggacggc gattatcttt ccgtgtatgt tccgaccacg ccgaatccga ccggcggtta
     3841 ctatattatg gtaaagaaaa gcgatgtgcg cgaactcgat atgagcgtgg acgaagcatt
     3901 gaaatatgtg atttcgctgg gtatggtcat ccctgacgac ctgcccgtca aaacattggc
     3961 aggacctatg ccgtctgaaa aggcggattt gcccgaacaa caataaagcc gccgttcaga
     4021 cggcattttc tgttttcagt ttaaatcaat aaaaggtgaa tttatgcgta ccaactattg
     4081 cggcctgatc agcgagcaat acttagacca aaccgtaacc gtcaaaggct gggtacaccg
     4141 ccgacgcgac cacggcggtg tgatttttat cgacctgcgc gaccgcgaag gcatcgtcca
     4201 agtcgtgatt gaccccgaca ctccggaagc gtttgctgct gccgattccg cccgcaacga
     4261 atacgttttg agcattaccg gccgcgtacg caaccgtccc gaaggtacaa ccaacgacaa
     4321 aatgatttcc ggcaaaatcg aaattcttgc caaagaaatc gaagtattaa acgccgccgc
     4381 tacgcctccg ttccaaatcg acgatgaaaa catcagcgaa aacgttcgcc tgaccaaccg
     4441 cgttatcgac ctgcgccgtc cggtgatgca gcgcaacctg cgcctgcgtt accaagtggc
     4501 tat
//
LOCUS       DAWHXS010000128         4433 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWHXS010000128 DAWHXS010000000
VERSION     DAWHXS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.128"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(159..1268)
                     /gene="mltB"
                     /locus_tag="WIE22_001845"
     CDS             complement(159..1268)
                     /gene="mltB"
                     /locus_tag="WIE22_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ4127878.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1265..1447)
                     /locus_tag="WIE22_001846"
     CDS             complement(1265..1447)
                     /locus_tag="WIE22_001846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127879.1"
                     /translation="MPSERGSAFRRHFRRRQADFGRRKRQRAAFCKAADCGGYRISRR
                     ALAPLSHLPYLFGTYL"
     gene            complement(1469..2896)
                     /locus_tag="WIE22_001847"
     CDS             complement(1469..2896)
                     /locus_tag="WIE22_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ4127880.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3081..>4433
                     /locus_tag="WIE22_001848"
     CDS             3081..>4433
                     /locus_tag="WIE22_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ4127881.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     F"
BASE COUNT          941 a         1238 c         1222 g         1032 t
ORIGIN      
        1 atgcaaaaat gccgtccgaa ccgaaacgtg gttcagacgg cattttatag tggattaaca
       61 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcaaggcaa
      121 cgccgtactg gtttaaattt aatccactat aaaaaacatt acaatcccgg gccgccaagc
      181 gaattggcaa tgtccctgac cgccgttaca tacatccgac tgtgattgta ctgccatacc
      241 gtataaaaat tgttcaagcc caaataatat tcaaacacgc cgggtgcggt ttccagtttg
      301 aacaaaaccg ccttttcatc atctgcaagc tcttcgccgg ggatgatgcc gtacgccttt
      361 aaatccgcca ccgtccgcgt cagggcggtt ttttcgccaa tgattgcctg aacatccgca
      421 cccggcgcca atgttgcaga caccagtatt ttcccgcccg tgcgccaacc gtgctgcttc
      481 atataattgg caaccgatgc cgcgacatcg ccgatgttgc cccatatgtc ccgatgtccg
      541 tccccgtcat aatccaccgc ccatttccgg tagctcgaag gcataaattg cggcatcccc
      601 attgcgcccg catagctgcc tttaaaggcg aaaacatcgc cgccttcttc ttttgccagc
      661 tttaaaagct cgaccaattc tttttggaaa aacccggcgc ggcgggggta atcaaagcct
      721 aaggtcgcca atgcgtccgc cacacggaaa ctgcccgtat ttttgccgta attcgtttca
      781 atcccgataa ccgccacgat aagttcggca ggcacgccgt atttttgcgc cacatcatcg
      841 ataagcgcgc ggttttccgc ataaaaccgg cgcgcgccgc gaaatttcgc cttgcccgaa
      901 tttcccgtgc ggaacacata ccacggacgc gatgtggagg ggcggtgcat aatcttgacg
      961 atgtccgcct tgtaagccgc tttgtcaaaa aaatcctgcc attccgcccg ggaaaaatcc
     1021 cctttcccga cttcatcgtc cacaaaacgg cggacatttg cattggcggc aaacccgctg
     1081 tcggataccg gtacggctgc cgcgtcaaat acggctgccg cgtcaaacgc ggggcggctt
     1141 tcttttttca tttcatccgc gcggggggct tgggcttcat ttgcccgggg cgggcgtgcc
     1201 tccatcgccg tacaggcaga caaagccgcc aaacaaattg ccagcggcag tatttttctc
     1261 tttttcataa atatgttccg aacaaatagg gtaagtggga aagcggcgca agggcgcggc
     1321 gcgaaatgcg gtatccgccg caatcggcgg ctttgcagaa tgccgcgcgt tgccttttgc
     1381 gccgcccgaa atctgcctgc cgccgccgaa aatgccgtct aaaggcactt cccctttcag
     1441 acggcattgc ctgcgccgta tttgcccgct atccgcaata tcggcagtcc aatatgtctt
     1501 tgcggatatc gttcagcagg caggctgccg cgtcttcgtg ttccgcctgt acgaagacaa
     1561 agagtcgggc gatgattttg ccgataaaga ctttttgcag ggcgcaggcg tcggtcagcg
     1621 tgtgttcctg caggaagctg cggatgtctt cgggtaaggt gtaatcacgg gcaacggcgg
     1681 caaagcgggc atcggtttgc aggcggtcga gcagggtttg gtttttgtcc aacttcatgc
     1741 ctgcttcttt ttcttcggcg gtggcgcgga caaactggtc gtaaatttcg gcataaagca
     1801 tatcgttcgg gccttcaaaa atcgtgaagg ggcggatgtc gatagcgata ttgccggcgg
     1861 tgtgtccgcg ttcaaaaccc ttcgcgccca agagtttttg caacatttgc gcggcggcgt
     1921 aagtgtattc cgtggcgagg gttttgacga tgttcgcctc catcagctga tgggcgacgg
     1981 gggcaacagg cgaaacggaa tggcagacgt agcggtaaag aatctcggaa acctgatggc
     2041 ggcgccggat ttcgcggcgt tcgtaatcga cgaatctgat gtcgttgcgg acgtatcgtt
     2101 ccaggttttc aaggatgtat tccataatgc cgtgcgtcat gccgatcagt tgcaggcggc
     2161 tgcggataaa gatgttttgg aacgcgcgca aaccggcagc gtcgctctgg gagagtttca
     2221 tcacggcggt tgcaggcatt tcggcatcga tgcggttgac ggcgtaacgg acggcgcgca
     2281 agccttcgga tgcgagggtt tcgcagcgga tgtatgtttt ggggacgagc agcaggtcga
     2341 tgactttggc gagtttgccg tttttgcgtt ctttggcggc aacgaggagg aagtcgcttt
     2401 gcgagttgcc ctgccagtat tttgcggcgt tgacgtaaat ggtttgtccg tcggtatatt
     2461 cgtagtaaga ctgcatttct cgggcaatcg ccgcgccgga ggtttcgggt tcggtaacgc
     2521 ccaaaccacc gccctcgcct ttgaaaacca tgtccaaacc ctgtgcgatt tgcgcttcat
     2581 cgccgaactc ttgcagtggc tgcaacacca gcgcgccttc gatgccggta cgcagcgtaa
     2641 cgggcacgcc gtaatgcccc gcaatccgca agacttcctg aatttcaaac tggctgccct
     2701 tgcgcccgcc gtgttttttg tcgaggaagg gcaacagcaa acccgcctgc ttcaaggcaa
     2761 gccatttgtc ttcgggcagg tatcgcatca ggtcgatacc gtctgaaaaa atgcggcgga
     2821 atgcggattc gatgtgcttt aaaaaagcag ccgtgtccat agttgacggc tgcgcgctcg
     2881 gttcggtgtg tatcatcggc ttcctctgtc ggttcccatt aatcggcggc cggtcaaacc
     2941 gcctgccaca gtttagagtt gattttctaa actttaccac aaagtgcgcc gggcaacaat
     3001 ccgccgacct ttcagacggc atcgcgcccc ctcccgtgct aaaatgaccg tttgcatcac
     3061 tgtccgccga ttgccgcact atgacctacc ccatccccaa accccgtgaa aaatcccgtt
     3121 ggtccaatct ttcgcaaggc tcgctgccct tggctttggc gcgttatctg ccgcataagc
     3181 ggctcaaggt cgtgctgacc caagatgcgg aacaggcgct acgccttcag acggcatggc
     3241 ggtttttccg tccgcacgac acggcggtgt tcctgccgga ctgggaaacg ctgccttacg
     3301 agcgtttttc gccgcatcag gatttggtgt cggaacggct gtcggcgttg tggcagatta
     3361 agagcggcgc ggcggacgtg ttgttcgtgc cggttgccac ggcgatgcag aagctgccgc
     3421 ccgtgccgtt tctggcaggg cgcacgtttt ggctgaaaac agggcagact ttggatatag
     3481 gccgtctgaa aaccgatttg gtagatgcgg gctacaacca tgtttcccat gttgttgcgg
     3541 cgggcgagtt tgccgtgcgc ggcggcattg tcgatttgtt ccccatgggc agcgaaatgc
     3601 cctatcgcat cgatttgttt gacgatgaaa tcgacagcat caaaaccttt gatacggaca
     3661 cgcagcgcac catctccccc gtttccgaaa tccgcctgct gccggcgcac gagttcccca
     3721 ccgacagcga agcgcaaaaa atcttccgca gccgcttccg agaagaagtc gacggtaatc
     3781 cgaacgatgc ggctgtgtac aaagccgtca gcaacggcca cttcggcgcg ggcgtggaat
     3841 attatctgcc gctgtttttt gaaaatgagc tggaaacgct gtttgactat atcggcgaag
     3901 acgcgctgtt tgtctctttg ggcgatgttc acgccgaggc aaaccgcttt tggagcgatg
     3961 tcaaatcccg ttacgcgatg gcgcagggcg acgaaaccta tccgcctttg cttccacagc
     4021 atttgtatct ctctgctgat gtttttgcag gccgtctgaa aaactacgga caagtgctgc
     4081 ccgatgtttc cggcaaggaa cacgccctcc ccgaccttgc cgtcaaccga caatccgacg
     4141 agcctttgca ggcattgaag gattttcaga cggcgtttga aggacggatt ttgctgtgcg
     4201 ccgaaagttt gggacggcgc gaaaccatgc tcggtttctt gcagcaaaac ggtttgaaag
     4261 ccaaacctgt gtccgactgg cagggcttct tgtcggcgca tgagccgctg atgattacag
     4321 ttgcgccgtt ggcatacggg ttcaaactgg gcggactgca atcgccgaac caacagcaaa
     4381 ctacttccgc ctccgaagga gaaggcgatg cggttaccga ccaaaccgaa ttt
//
LOCUS       DAWHXS010000129         4384 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWHXS010000129 DAWHXS010000000
VERSION     DAWHXS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.129"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            449..3628
                     /locus_tag="WIE22_001849"
     CDS             449..3628
                     /locus_tag="WIE22_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ4127882.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGAQAQTHKYAIVMNAQ
                     NLPEVKWGQDYRKLAQKSNERQFTHTTNFYIKKNVTLSFNNIDEVVAEKKDVVVFGTA
                     TYLPPYGKVSGFDADKLKKRGDALGWIKTIKPGLVGYSYEGVTCQNNYNNASRGCPEL
                     IYKTQFTFGNSGLAKKANGGGLDIYVDKSRENSPIYKLQDYPGLGVSFNLSSESLVKS
                     VKYNKIISSFSEDVTQQNGTQSHHKDKNLVYTTGDYQYKNRYSSRYVGQDEHSAVAFY
                     LNAKLHLLDKKNIKNIAQGKTVNLGTLKPYVEPTEEWKNKRGSHFQGNWTFEDKGEVS
                     VKLKLPEVKAGRCINANNPNPNAKAPSPALTAPALWFGPVKDGKAEMYSASVSTYPDS
                     SSSQIFLQNLSRKDDTSKPGRYSLKPLSTSEIKSKEPNFTGRQTIIRLDGRVQQIKLG
                     QSNNEVVGFNGNSNNATFGIVSEGSFMPDTSEWKKVLLPWTVRVFADDSKFKEFNKEE
                     KDNKPKYSQKYRSRDNGKRERNLGDIVNSPIVAVGEYLATSANDGMVHIFKKNGGGDD
                     RNYSLKLSYIPGTMPRKDIESKDSNLAKELRAFAEKGYVGDRYGVDGGFVLRQVEWKG
                     QNRVFMFGAMGFGGRGAYALDLTKADDNDPTKASLFDVKDNGNNGNNGNNGVQLGYTV
                     GTPQIGKTHDGKYAAFLASGYATKDINNGENKTALYVYDLESNNGTLIRKIEVPNGKG
                     GLSSPTLVDKDLDGTIDIAYAGDRSGKMYRFDLSSQSPDQWTVRPIFEGTKPITSAPA
                     ISQLKDKRVVIFGTGSDLSEDDVDNTDEQYIYGIFDDDTATTGSVNFSGSGGGLLEQH
                     LTQEDKTLFLTDYKRSDGSGSKGWVVKLKDGQRVTVKPTVVLRTAFVTIRKYTDNGCG
                     AETAILGINTADGGKLTKKSARPIVPEANQAVAQYSGDKKTSSGKSIPIGCMEKNGGT
                     VCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRAGKNNRCFSQKGVR
                     TLLMNDLDSLDITGPMCGMKRISWREVFF"
BASE COUNT         1231 a         1028 c         1155 g          970 t
ORIGIN      
        1 ttaggtttct gattttgttt ttctgttttt gtaggaatga tgaaattttg agttttagga
       61 atttatcgga aaaaacagaa accgctccgc cgtcattccc gcgcaggcgg gaatccggct
      121 tgttcggttt cggttttttt tgaggtttcg ggcaacttct aaaccgtcat tcccgcgcag
      181 gcgggaatcc ggcttgttcg gtttcggttt tttttgaggt ttcgggcaac ttctaaaccg
      241 tcattcccgc gcaggcggga atccagaccg ttgggcatct gcagcggttt gctaaaaaac
      301 gctttaccgt gatcagtgtg caaagttaaa atggggaggt aagcttttca atcagcaatt
      361 cggcgggcgc ggaatcgggc ggtttaccga accccggcgt tcgcggcgcg ccgccccgtc
      421 ccgtgaaggc aaactcaagg aataaaagat gaataaaact tggaaacggc aggttttccg
      481 ccataccgcg ctttataccg ccatattgat gttttcccat accggcgggg ggggggcgca
      541 ggcgcaaacg cataaatacg ctattgtaat gaacgcgcaa aatctgcctg aggtaaagtg
      601 ggggcaggac tatagaaaat tggcgcaaaa aagcaatgaa cgccaattta cccatacgac
      661 taatttttac atcaaaaaaa acgtaacttt atcattcaat aatatcgatg aagttgttgc
      721 tgaaaaaaaa gatgttgtcg ttttcggcac agccacctac ctgccgccct acggcaaggt
      781 ttctggtttt gatgccgata agctgaaaaa gcgcggcgat gcccttggtt ggattaagac
      841 gatcaaaccg gggttggtag gctacagcta cgaaggtgtc acatgccaaa acaactacaa
      901 caacgccagc cgcggctgtc ctgaacttat ctataaaacc caatttacct tcggtaattc
      961 cggtttggca aaaaaggcaa acggcggcgg gctggatata tacgtagaca aaagccgcga
     1021 aaattcgccc atttacaaat tgcaggatta tcctgggttg ggcgtgtctt tcaatttgag
     1081 cagcgagagc ctcgtcaaat cagtaaaata caataaaatt atatcttctt ttagcgagga
     1141 cgtgacgcag caaaatggta cgcaaagcca ccacaaagac aaaaacctcg tttatacgac
     1201 aggcgattac cagtataaga atagatattc ttcccgttat gtcgggcagg acgaacactc
     1261 cgccgtcgct ttttatctga acgccaaact gcacctgctg gataaaaaaa acatcaaaaa
     1321 tatcgcgcaa ggcaaaacag ttaatttggg taccttgaaa ccgtacgtcg agccgacgga
     1381 agaatggaaa aacaagaggg ggagccattt tcaaggtaat tggacgtttg aagataaagg
     1441 agaagtcagc gtcaaactca aattgccgga agtcaaagca ggccgctgca tcaacgcaaa
     1501 taaccccaat cccaacgcca aagccccctc ccccgcactg accgcccccg cgctgtggtt
     1561 cggacctgtg aaagatggta aggcggagat gtattccgct tcggtttcta cctaccccga
     1621 cagttcgagc agccaaattt tcctgcaaaa cctttcccgc aaggatgaca caagcaaacc
     1681 gggccgctat tccctcaaac ccttgagtac gtcggagatt aaaagtaaag agccgaattt
     1741 cacggggcgg caaacaatca tccgattgga tggcagagta cagcaaatca aactgggtca
     1801 aagcaataat gaggtcgtcg gttttaatgg aaacagcaac aacgcaactt tcggcattgt
     1861 tagtgaaggg agcttcatgc ctgataccag cgagtggaaa aaagtgctgc tgccttggac
     1921 ggttcgggtt tttgctgatg acagtaaatt taaagaattc aacaaagaag aaaaagacaa
     1981 caagccaaaa tacagtcaaa aataccgcag ccgcgacaac ggcaagcgcg agcgcaattt
     2041 gggcgacatc gtcaacagcc ccatcgtggc ggtcggcgaa tatctagcta cttccgccaa
     2101 cgacgggatg gtgcatatct tcaaaaaaaa cggcggcggg gatgaccgca actatagtct
     2161 gaagctcagt tatatcccgg gcacgatgcc gcgcaaggat attgaaagca aagactccaa
     2221 cctcgccaaa gagctgcgcg cctttgccga aaaaggctat gtgggcgacc gctacggcgt
     2281 ggacggcggc tttgtcttgc gccaagtcga atggaaaggg caaaaccgcg tgtttatgtt
     2341 cggcgcgatg ggctttggcg gcagaggcgc gtatgccttg gatttgacca aagccgacga
     2401 caatgacccg acaaaagcct ctttgtttga tgtaaaagat aacggcaata atggcaataa
     2461 cggcaataac ggcgtgcaat taggctacac cgtcggcacg ccgcaaatcg gcaaaaccca
     2521 cgacggcaaa tacgccgcct tcctcgcctc cggttatgcg actaaagaca ttaacaacgg
     2581 cgagaataaa accgcgctgt atgtgtatga tttggaaagc aacaacggta cgctgattag
     2641 aaaaatcgaa gtacccaacg gcaagggcgg gctttcgtcc cccacgttgg tggataaaga
     2701 tttggacggc acgatcgata tcgcctatgc cggcgatcgc agcgggaaaa tgtaccgctt
     2761 tgatttgagc agtcaaagtc ctgatcaatg gactgtacgc cctatttttg aaggcacaaa
     2821 accgattact tccgcgcccg ccatttccca actgaaagac aaacgcgtgg ttatcttcgg
     2881 cacgggcagt gatttgagtg aggatgatgt ggacaatacg gacgaacaat atatttacgg
     2941 tatcttcgac gacgatacgg cgacgacggg ttctgtaaac ttcagcggtt cgggaggcgg
     3001 gctgctcgag caacacctta ctcaggaaga taaaacctta ttcctgaccg attacaagcg
     3061 atccgacgga tcgggcagca aagggtgggt agtgaaattg aaggacggac agcgcgttac
     3121 cgtcaaaccg accgtggtat tgcgtaccgc ctttgtaacc atccgcaaat atactgacaa
     3181 cggctgcggc gcggaaaccg ccattttggg catcaatacc gccgacggcg gcaagctgac
     3241 gaagaaaagc gcgcgcccga ttgtgccgga agccaatcag gctgtcgcgc aatattccgg
     3301 cgataagaaa acttccagcg gcaaatccat ccctataggt tgtatggaaa aaaacggggg
     3361 aaccgtctgc ccgaacggat atgtttacga caaaccggtt aatgtgcgtt atctggatga
     3421 aaagaaaaca gacggatttt caacaacggc agacggcgat gcgggcggca gcggtataga
     3481 ccccgccggc aagcgtgccg gcaaaaacaa ccgctgcttc tcccaaaaag gggtgcgcac
     3541 cctgctgatg aacgatttgg acagcttgga cattaccggc ccgatgtgcg gtatgaaacg
     3601 aatcagctgg cgtgaagtct tcttctgatt tgcacgcgaa aatgccgtcc gaaaggtttt
     3661 cggacggcat tttttgcgtt tttcgggagg ggcgggttcg taaaaggcgg gctatagggt
     3721 aggcttcatc tcgccaatct cactgaatcc atcaatttcc acaattcaat taaataccgt
     3781 caaaccgatg ccgtcattcc cgcgcaggcg ggaatctaga cattcaatgc taaggcaatt
     3841 tatcgggaat gactgaaact caaaaaactg gattcccact tccgtgggaa tgacgggatg
     3901 caggttcgtg ggaatgacgt ggtgcaggtt cgtaggaatg acgtggtgca ggttcgtagg
     3961 aatgacgtgg tgcaggttcg taggaatgac gtggtgcagg ttcgtaggaa tgacgtggtg
     4021 caggtttccg tgcggatgga ttcgtcattc ccgcgcaggc gggaatctag acattcaatg
     4081 ctaaggcaat ttatcgggaa tgactgaaac tcaaaaaact ggattcccac tttcgtggga
     4141 atgacgggat gtaggttcgt gggaatgacg ggatgtaggt ttccgtacgg atggattcgt
     4201 cattcccgcg caggcgggaa tctagacatt caatgctaag gcaatttatc gggaatgact
     4261 gaaactcaaa aaactggatt cccactttcg tgggaatgac gggattagag tttcaaaatt
     4321 tattctaaat agctgaagct caacgcactg gattcccgcc tgcgcgggaa tgacgaattt
     4381 tagg
//
LOCUS       DAWHXS010000130         4375 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWHXS010000130 DAWHXS010000000
VERSION     DAWHXS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.130"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..1451)
                     /locus_tag="WIE22_001850"
     CDS             complement(<1..1451)
                     /locus_tag="WIE22_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ4127883.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHK"
     gene            complement(1565..2875)
                     /locus_tag="WIE22_001851"
     CDS             complement(1565..2875)
                     /locus_tag="WIE22_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ4127884.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            3187..3600
                     /locus_tag="WIE22_001852"
     CDS             3187..3600
                     /locus_tag="WIE22_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127885.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASEKNTDKQ"
     gene            3694..4375
                     /locus_tag="WIE22_001853"
                     /pseudo
     CDS             3694..4375
                     /locus_tag="WIE22_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT         1125 a          994 c         1133 g         1123 t
ORIGIN      
        1 tatttatgga tgtagtcgcc gaagagttcg cgggtattga cgttgcctgc ggttacgccg
       61 ataagcggcg tgccagtcag cccgattttg acggcgttta cgtcggactg gttgaggttg
      121 gcaagaaacg agcctttggg gttgtagctg cggtgtactt cgtcgagaaa atagacgcgc
      181 tgaatggcga ggtcgtagtc gttacgagcg acgacatcgg ggtcgtcttg gaatttttgg
      241 atattaacaa cagtgatttc cgctttgcct gcgtggttgt gcaaagtttg ggcggatttg
      301 atgtcggcag caaaggcttc gcggctgtcg atggtatgga cgactaaatc gcgggcagta
      361 aattcgcgct gcgcctgttt caataaatca agcctgtcca cgatgaaata gaatttcggc
      421 acgatgccct gtttggcata atagtgggtc aggtagcggg tgttgtaata ggcaagcgcg
      481 gttttgccag agccttgggt gtgccaaatc acgccttttt tgccgccgtt tgccaaatgt
      541 ttttcaatgg cgagagtggc aaatagctgc ggataccgca tgatgtgttt ttgcaccaag
      601 ccttgacttg ctttgacgta agtcagcccg tgttgcagaa gaaaagaaag acgttcgcgg
      661 caaagcagcg aagtcaggat gcggttggtc ggcgtatcgg gcgatttatt gctgataaat
      721 tcggggctgt gtttgataac gggcaggttg ttgtcttgca ggacgttgtt ttcgaggccg
      781 tctgaaagcg tgttcaataa ttcggcaaga tttaatttat gttcttcgcg gaagtagtta
      841 aacacgggct taccgtaagc agacgaagcg taaaacgcgc cttgtgccgg ttcggttgcg
      901 ccgtcgtcgt attccatgtt gttggaaaaa atcatgaatt gggtgatgtt gatgaaacgg
      961 cgaaatttgg aatttttcgc acgtttgccc atgcgctccc gctcttcgcc gattccgcct
     1021 ttgttgttgg gctttttgac ttcgataaaa accaaaggca tgccgtttac cagcagggta
     1081 atgtcggggc ggaattcttc atcgccgttg atacagggca attcggttac aacgtggaag
     1141 ctgttgttat caaaattttg gaagtcgatc agtttcttgc cgccggattg attggtaagg
     1201 cgttcgtaaa acttttgccc caagtcttcg ttgtccagtt ctaagcgtat gtcggtcagc
     1261 aaacggcgtg catcgtccgg cgacaaatcg ggattgatgc ggcagaggct ttctataaag
     1321 attttgggaa agatgttggt tggtttatcc caattttcgt ttttgagcga gaggtagtcg
     1381 tagcccaagc gcatcaggtg caaaacggcg gggattttga cgcgggaatt ttcgttgtga
     1441 gtttgggaca taaacgtaat gattcatcaa aatttattga aacggattat aacacctgaa
     1501 aaacccgata cgcctttttg gtgtaccggc tttgccatac cgttctgctt cagacggcat
     1561 tgcttcattt tgcctttaat gcctcttcat ccagcgattt gagccacgcc agcttttcgc
     1621 cgattttgat ttccaacccg cgcgggacgg gttggtagaa gtccggttcg tccaagccgt
     1681 cgggcatata gctttcgccg gcggcgtagg cgttcggttc gtcgtgggcg tagcggtatt
     1741 cgcgtccgta gcccaattcc ttcatcagct tggtcggggc gttgcgcagg tggacgggca
     1801 cttcgtcgct agcgttttct ttgacgaagt ggcgcatttg gttgtatgcc ttgtagcccg
     1861 cgttggattt cgcggcggcg gcaagataca ataccgcttg cgccaaagcc agttcgcctt
     1921 cgggcgagcc taagcgttcg aaggtggcgg cggcatcgtt ggcgatttgg aaggcgcgcg
     1981 ggtcggcaag cccgatgtct tcccaagcga tacgcacgat gcggcgggcg aggtagcgcg
     2041 ggtcggtgcc gccgtcgagc atacggcaga accaatacag cgcggcgttc ggatgcgaac
     2101 cgcgcacgga tttgtgcagg gcggagattt ggttgtagaa actctcgccg cctttgtcga
     2161 aacggcggat ttgcgccccg agactgtcgg cgagaaattc ggcggttaag tttttcagac
     2221 gacgtgtatc ggcggcgcgt aaaagttgtt ccaacaaatt caacaatctg cgcgcatcac
     2281 cgtcggcggt attcacgagt aatttttgcg catccgtttc aatcgtaaac tcttggtatt
     2341 caggcaaagc caataccttg gcaatcagct ttttcaggtc gtctgaagac aagggttgca
     2401 aaacatacac ctgagcgcgg ctcaacagcg cgggattgac ttcaaacgac ggattttccg
     2461 tcgtcgcacc aataaaggtt agcaaaccgc tttcgacatg cggcaaaaac gcgtcctgct
     2521 gcgccttgtt gaagcggtgg acttcatcga caaacaaaat cgtcgcgcgt ccctgctgca
     2581 aagcgatttc ggctttatcg attgcctcgc ggatgtcctt cacgccggaa aatacggcgg
     2641 aaacaggcaa aaactgggcg ttgaaactct gcgccaaaat ccgcgccaac gtcgtcttgc
     2701 ccacgcccgg cggcccccac agcaacatag aatgcggctt gccgccttct accgccacgc
     2761 gcaaaggttt accttcgccg atgaggtgtt cctgccccac cacgtcgtca agcgtatgcg
     2821 gacgcaatcg ttcggcaagc ggcgcgtcgg gttctcgggc aaacaaatcg gtcataacgg
     2881 ctccgtcaac aggttttcaa acaatatgat tatacggcag ggaacggcgg cgtgccgcat
     2941 acggattccg cccctccgtt tgccttaagc cgatattagg cgcatactgg aaaagacgag
     3001 agacttcaca caatatatcc ggcacggaga ccgattccgc atcggcatga caatacccaa
     3061 atcagcgttt caattaaaca ttaaggagac taaaatagaa aatttgcttt atctaccatt
     3121 gctttgttga ttcaatcggc attatgtttt gaggcggaag cccatgaata tactaatatt
     3181 caagagatgg aatgggtgtc tttattttct gatccgcaaa gagacgatga tagtcttata
     3241 acccttaaag atgaaaaaat cactgtaaaa aactatattg tgccttggtg gaaaaaaggt
     3301 gaaaacttta gaaaattaga acttattacc actggtaaaa atgataatgg aaatttaacg
     3361 gtaaatggaa tttacggcat ccaatctttt gcagactctc aattgggata tacgcctgaa
     3421 ttattggtaa aaagcaataa tgatattcta tttaaaaata atctccaatc tgttgacgtg
     3481 aagccccatt ccggaaatat ccatttggat gccaaaggaa aaattcaatt taatttgaat
     3541 gttcccgatg ttgatggggt acaagatgta gccagtgaaa aaaataccga caaacaatag
     3601 tgccttagtt cggtatgtca acactatcag ccccccgatg aaaatgacaa aggcagcata
     3661 tcctttcatt caaaaacagg caatttgttc aatatgaacg ttaaagttca taaagacaca
     3721 caccatttcg gtattcacaa cctcaacaat accgatgttc atctgactgc cgaagacagc
     3781 gataatgtga ttgagattaa aggggaactc gccgacccta tgccgacagg aaatgttgag
     3841 agtacaagtt ttttaagggg cattgaaacg gcttttaaat ccaataccgt attaacgggg
     3901 aataacaata aaatatccat cgccactgag ggcaagggct tgggattggg cgtaaatggt
     3961 ctccagtcca actcccccta ttccggacac acgacgggca ttttcatcaa taatcaatca
     4021 agcgacctta cccaaccttc ccttgatggc atcagcacca tcaaactcaa agccgtcaag
     4081 ggaaataatg aagttgattt aaatgtaaaa aaccacgcct ccgtaaaagg cattatcgcg
     4141 tcgcattctg caaaagcaac gcttgaagcc gaaggggaca atattgtcca ggttaaaaat
     4201 ccggacacca atacggcttt aatcgatgct ttgaaaaaac aataccccaa tgcttcgaaa
     4261 gaactataca gggtgggggt tcaatccacg actggaacag tacgcatttc gccaaacggc
     4321 aaaatcgcat taacggaatt gaaaactttt ggaattgtac aaaacggcat ttgcg
//
LOCUS       DAWHXS010000131         4343 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWHXS010000131 DAWHXS010000000
VERSION     DAWHXS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.131"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(402..1253)
                     /locus_tag="WIE22_001854"
     CDS             complement(402..1253)
                     /locus_tag="WIE22_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ4127886.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1601..3115
                     /gene="katA"
                     /locus_tag="WIE22_001855"
     CDS             1601..3115
                     /gene="katA"
                     /locus_tag="WIE22_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ4127887.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARANDPALGQPGLL"
     gene            3191..3412
                     /locus_tag="WIE22_001856"
     CDS             3191..3412
                     /locus_tag="WIE22_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127888.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGNLERKI"
     gene            3733..4299
                     /locus_tag="WIE22_001857"
                     /pseudo
     CDS             3733..4299
                     /locus_tag="WIE22_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
BASE COUNT         1009 a         1156 c         1156 g         1022 t
ORIGIN      
        1 atccaggttc gttgagtttc ggtcatttcc gataaattct tgcagctttg cgtttctaga
       61 ttccctcttt cgcgggaatg acgacggagg gttgctgttt tcccgacaaa ttcccgccat
      121 ctaaaatctc gttatccata caagaaccga aaatcttgcc attctcacaa aaacagaaat
      181 tcaaaaacag aaatcccaaa ccctcggcat tcccgcgaaa gcgggaatcc aggttcgttg
      241 agtttcggtc atttccgata aattcttgca actttgcgtt tctagattcc ctctttcgcg
      301 ggaatgacgg gatgcaggtt tccgcgcgga cggattcggc attcccgctt tcgcagaaat
      361 gccggctttt ggtttgttgt ctttataaga ttattcggca attattctgc ggttttgcgt
      421 ttgtgtgctg ccggaatctc aaaggattct ctgtatttgg caatggtgcg gcgagaaacc
      481 tccatgccgc ggaaagccag caggttggcg agcgcctcgt cagaatacgg cgtgggtgtg
      541 gacgaggcaa aaccggcggc gggaaaggga ttgagcgaag caatgaggtc gagtgcggct
      601 tcgagtgtgc cgctgtcggt ttggggcagg cgttttttta ttcgcgcgag ggtttggctg
      661 cggttgccgt caatgctgtc gagggcgttt cggacgatat gcagggcgga gggtttggca
      721 gcacattcgc ccgatctttc tatctgcagt atcagcgatt cgttcaaatc ggcggcggcc
      781 acgcctgccg ggtcgaattt tttcaatgcg gtcagggctt gtttcagcat tgcttcatcc
      841 aacatccact ccaagggcgt atggtcgagg atgtcttcga tgctgtcggt cagataacct
      901 tgctcgtcaa ggaaatcgat gaggatgtgg acacaggcgg attcttggtc ggaaagcggg
      961 tgttcgcata cttgcgcgtg caggtattgc ttgaaatcct gctcgccggc gatgttggac
     1021 agcatatctt cgccttcgtc tccgccgatt tgacgggcag gcgcagtgta atggctgaac
     1081 tcggcatcgg aaaattcatc cgtgtctttg cgttcgagca gggggttgtc cgacagccag
     1141 ttttcgacct cgcgttcaag ttcgataccc gacatctgca atacgcgcaa agattgttgc
     1201 agccgctggt tgagctgctg ggtctgtttg agctttattc cgagtaaggt catgataatg
     1261 tggggaaaat tgttattttc agcctgtcgg cgcaaaaaat gccgcaaagc gtcattgcat
     1321 tataaatggt tttaatgagc gggttcggat tccgttcgat aacaaaaaac aaacgaaaat
     1381 caagaaccga ttgcttataa taatattaaa tcgatttcat agttttaata gcgaaaatct
     1441 tggcgtatag tcgcatccat agtttttaca aaagggaaat aaaatgtcga ttcaagaaat
     1501 ttattgcaat caagaaaccg gttacgaata cgctttccgc caaatcgtac tgtaagccgt
     1561 ttacccccat ttgaccaacc tgagaaaagg aacaagagcg atgactacct ccaaatgccc
     1621 tgtaacccat ctgaccatga acaacggcgc gcctgttgcc gacaatcaaa acagcctgac
     1681 cgccggtcct cgcggccctc tgctggcgca ggatttgtgg ctgaatgaaa aactcgccga
     1741 cttcgtgcgc gaagtcatcc ccgaacgccg tatgcacgcc aaaggttcgg gcgcgttcgg
     1801 tacgtttacc gtaacgcacg acatcaccaa atacacccgc gccaaaatct tcagcgaagt
     1861 cggcaaaaaa accgagatgt tcgcccgttt caccaccgtg gcaggcgaac gcggcgcagc
     1921 cgatgcagaa cgcgacatcc gcggttttgc gttgaagttc tataccgaag aaggcaactg
     1981 ggatgtggtc ggcaacaaca cgcccgtgtt cttcctgcgc gacccgcgta agttccccga
     2041 cctgaacaaa gccgtcaaac gcgacccgcg caccaatatg cgctctgcca caaacaactg
     2101 ggacttctgg acgctgctgc ccgaagcact gcaccaagtt accatcgtga tgagcgaccg
     2161 cggcatcccc gccggctacc gccatatgca cggcttcggt tcgcatacct acagcttctg
     2221 gaacgaagca ggcgagcgtt tttgggtgaa attccatttc cgcacccaac aaggcattaa
     2281 aaacctgacc aacgaagaag ccgccaaaat catcgccgac gaccgcgaaa gccatcagcg
     2341 cgacttatac gaagccatcg aacgcggcga gtttccgaaa tggacgatgt acatccaagt
     2401 catgcctgaa gcagacgcgg aaaaagtacc ttatcatccg tttgacttga ccaaagtttg
     2461 gccgaaaaaa gactatccgc tgattgaagt gggcgaattc gagttgaacc gcaatcccga
     2521 aaacttcttc gccgatgtgg aacaatccgc cttcgcaccg agcaacctcg ttcccggtat
     2581 cggcgcgagc cccgacaaaa tgctgcaggc gcgtttgttc aactacgccg acgcgcaacg
     2641 ctaccgcttg ggcgtgaact tccgccaaat ccccgtcaac cgcccgcgct gccctgttca
     2701 cagcaaccaa cgcgacggac aaggacgcgc cgacggcaac tacggcagcc tgccgcacta
     2761 cgaacccaat agcttcggcc aatggcagca acaacccgac ttcgccgaac cgcctttgaa
     2821 aatcaacggc gacgcggcgc actgggacta ccgccaagac gatgacgact atttcagcca
     2881 accgcgcgcc ctgttcaacc tgatgaacga cgcgcagaaa caggcattgt tcggcaacac
     2941 cgccgccgca atgggcgacg cgcccgactt catcaaatac cgccatatcc gcaactgcta
     3001 ccgttgcgat ccggcatacg gcgaaggcgt ggcaaaagcc cttggactga ctgtcgaaga
     3061 tgcccaagcg gcacgagcca acgacccggc tttaggtcaa cccggtttgc tgtaaggaag
     3121 cattatgtgg atagaaattg aagaaatcct gtcccgcgcc gtccgctacc ggaaataacc
     3181 gggcataaaa atgccgtctg aaacgtcgtt tgaccgcttc agacggcatt tccccatccc
     3241 gcccccgccg tttcagtggg cgttttttat taaacgcaaa atatcccgtc attcccgccc
     3301 cacagcccac ttttccgccg tcattcccgc accatcccca ccggttcaaa ccggcacgaa
     3361 aacttttccg cgtcattccc gcgaaagcgg gaatctagaa cgtaaaatct aaagaaaccg
     3421 tttttcccga taagtttctg caccgacaac tctggattcc cgcctgcgcg ggaatgacga
     3481 atccatccgc acggaaacca aaatcccgtc attcccacga aagtgggaat ccagaacgta
     3541 aaatctaaag aaaccgtttt tcccgataag tttctgcacc gacaactcta gattcccgcc
     3601 tgcgcgggaa tgacgggatt tctgtttttg attttttgtt tttgcgggaa tgacgggatg
     3661 tgggttttcg tgcggcgttt ttgcattttt ttgcttttgc tataatccgc cctttttgag
     3721 gacgggtgcg gtatgggttt ttatgctttg ctcttgattg cgctggggat gtcgatggat
     3781 gcgtttgccg tcgcattggc aaagggtgcg gcggtcagaa tgcctccgcg caaaattgcg
     3841 gcaacggctt tggtgttcgg cacggttgaa gcgctcacgc cgctggcagg ctgggtgggc
     3901 ggtttttatg ccaagccgtt tatcagcgaa tgggaccatt gggcggcttt cgtcctgctg
     3961 ggcgggctgg gtctgaagat gttgcgtgag ggcttgtccg gcggggcgga cgatgtgcgc
     4021 gaagccaaac gggaaagctg gtgattgacg gttctgactg cttttggaac cagtattgat
     4081 tccatgatag tcggggtggg cttggcgttt atggaggtaa acatcgcctt tgccgccgcc
     4141 gtcatcggca tggcgacgac ggtgatggtt acggtcgggc tgacggcggg aagggctttg
     4201 ggcgtattgt tcggcaaacg cgccgagttt gccggaggtt tggtgttgat tgccatcggt
     4261 acatggacgc tcttgtcgca tttgggtttg attcaatgat gtcggaaaat atagtggatt
     4321 aacaaaaacc agtacgtcgt tgc
//
LOCUS       DAWHXS010000132         4154 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWHXS010000132 DAWHXS010000000
VERSION     DAWHXS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.132"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(193..264)
                     /locus_tag="WIE22_001858"
     tRNA            complement(193..264)
                     /locus_tag="WIE22_001858"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Arg,seq:cct)
     gene            complement(284..730)
                     /locus_tag="WIE22_001859"
     CDS             complement(284..730)
                     /locus_tag="WIE22_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ4127889.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(735..1709)
                     /gene="ispB"
                     /locus_tag="WIE22_001860"
     CDS             complement(735..1709)
                     /gene="ispB"
                     /locus_tag="WIE22_001860"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ4127890.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1725..1913)
                     /locus_tag="WIE22_001861"
     CDS             complement(1725..1913)
                     /locus_tag="WIE22_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127891.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1933..2241
                     /gene="rplU"
                     /locus_tag="WIE22_001862"
     CDS             1933..2241
                     /gene="rplU"
                     /locus_tag="WIE22_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ4127892.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2266..2538
                     /gene="rpmA"
                     /locus_tag="WIE22_001863"
     CDS             2266..2538
                     /gene="rpmA"
                     /locus_tag="WIE22_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ4127893.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2926..4110
                     /gene="ubiM"
                     /locus_tag="WIE22_001864"
     CDS             2926..4110
                     /gene="ubiM"
                     /locus_tag="WIE22_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ4127894.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT         1190 a         1148 c          911 g          905 t
ORIGIN      
        1 aaaacaacag atttccaagc cggaacatct tttacggagc ctgaaaatca tttcatgatt
       61 ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa gttttcagat ggcaaaaaag
      121 ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc aacacgggaa acagacaaat
      181 gaaatcgtca aacctcgcca acaggaatcg aacctgtatt ttacgcttag gaggcatacg
      241 ttctatccgt tgaactatgg cgagccgaaa tgaaaaggag attttaaccc tttccgacga
      301 caaaagacaa gatgccggcc gcaatcagcg gcccgacagg gataccgccc ataaatgcca
      361 ccccgataac cgtcccgatt aacagccctg taattaaaac aggctgctgt cccatcaaag
      421 gcacgccgcg tcccgccagc caagccacga aaataccgat aaaaacggcg gatatcattt
      481 taaaattcaa aaattcggca acgggaggaa cctgcgcctt tcctgaaacc aacggactca
      541 aaacccctat ggtcaaaaga atgataccga gattcaaccc atgcttctcg accaacggga
      601 caaactgtac caatgccgtc tgctgcatca gcagcaatat ggttgccgag atggtaatcg
      661 aattgttgtt gctgaccacc cccaacagaa tcagcgtaac cagaaacagg ggaacaaaac
      721 tgaaattcat cgcctcaaga caccctgacc aaagattcct tcgccagctg aatcatggca
      781 tccttcactt cgctgtcggg cagggcatcc aaggcggtaa cggcacaatc gactgctttg
      841 cgcgcctcgc ctatcgaata tgccaaagca tccgaacgga cgacataatc gtggattttc
      901 tcaaaatagc tgcgatctgc attttccaaa gcagtacgca catcgttcgc aacctgttcg
      961 gaaccctgac gcatcagata aatcaaaggc aaagtcggtt ttccttccgc caaatcgtcg
     1021 ccgacgtttt tgccggtttc ttcggtttcg ccagaatagt ccagcacatc gtcaataatt
     1081 tggaatgccg taccgacata cataccgtag tctttcaacg cccgttcgtg ttcgggggaa
     1141 gccttgccca aaattgcgcc gacctgcgcc gccgcttcaa acaattttgc cgttttatat
     1201 tggatgactt ggatatattg ttcttcggta atgtccgtat tgccgatgtt catcagctgc
     1261 atgacttcgc cctcggcaat aatgttggtt gcatccgcca tcacttccaa aacgcgcata
     1321 ctgcccgagg caaccatcag ttgaaaggcg cgcgtgtata aaaagtcgcc aaccaacaca
     1381 gccgccgcat tgccgaacag attgtttgcc gttgcccgcc cacggcgcaa atcgctttca
     1441 tcgacgacat cgtcgtgcag aagcgtcgaa gtgtggataa actcgaccat cgccgccagc
     1501 gaatacagtt tctcgtcatc ataaccgacc gccttacccg ccaaaatcgt cataatcgga
     1561 cgcaggcgtt tgccgcccgc gctgatgata tatgtaccga tttgcgaaat cagtgcgaca
     1621 tcggattgca ccgcacggtt gatgacttca ttgactttgg caaggtcttc aggcagatgt
     1681 cgctggaaat agggcagatt ctcgagcatg gcagactctc aggtttacgt ttccgtcaaa
     1741 cggcagatac cgcttgaaag ctgattcaaa tagcgggcaa ttatatcagc aaagccatca
     1801 gggtacatct tttcagacgg catatggtta tccgaaccgc gcaaactttg acaaaacagg
     1861 caaataacaa tacaatgccg agttccgcac aatggtgtgc ggatattaac cataaccctt
     1921 atggagttga gtatgtacgc ggtcgtaaaa actggcggca aacagtataa agtttccgtc
     1981 ggcgaaaaat tgaaagtaga acagatacca gccgaactcg acagccaaat cgaactgacc
     2041 gaagttttga tgattgctga cggcgaatct gtaaaagttg gcgcaccctt tatcgaaggt
     2101 gcaaaagtaa cggctaaagt agtggcacac ggtcgtggcg aaaaagtccg catcttcaaa
     2161 atgcgccgcc gcaaacacta ccaaaaacgc caaggccacc gccaaaattt cacccaaatc
     2221 gaaatcgtgg caatcgccta accttaaaac ttaggagcat tacaaatggc aagtaaaaaa
     2281 gcaggcggca gcacccgcaa cggtcgcgat tcagaagcca aacgcttggg cgttaaagcc
     2341 tacggcaacg agctgattcc cgcaggttcc atcatcgtcc gccaacgcgg caccaaattc
     2401 cacgcaggcg acaacgtagg tatgggcaaa gaccacactt tgttcgccaa agttgacggt
     2461 tatgtcgaat tcaaaaccaa aggcgcgctg aaccgcaaaa ccgtaagcat ccgtccttac
     2521 accggttctg aagaataatt caaccgaaca ggaagccgca tcttttcacg atgcggcttt
     2581 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
     2641 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
     2701 aggcaacgcc gtactggttt aaatttaatc cactatacat accccacacc cgatttgata
     2761 cacattaagc tgaaagtaaa aatccgcata caccctcccc tgcatatttc ttcaacagtg
     2821 ggttttgata taatcgccta tctgttacag atagttcaaa cggcattcaa acccttacaa
     2881 ataccgtctg aaatcatcgg catccgtcta tgcaaaggat attttatgag tttacacagc
     2941 gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt ttgcagccga acttgccggc
     3001 agcggtttga aagttaccct gatcgaacgc agcccactga ctgttctgca aaatcctcca
     3061 tatgacggac gcgaaatcgc cctgacccat ttttcccgcg aaatcatgca gcgtttgggt
     3121 atgtgggaca aaattcccga aaacgaaatc taccctttgc gcgatgccaa agtgctgaac
     3181 ggacgttccg actaccagct ccactttccc caaccgaccg aggcgcgcgg cgagcctgcc
     3241 gactgtttgg gctatctgat ttccaaccac aatatccgcc gcgctgccta cgaagtcgta
     3301 tcccaactcg acaacattac cattctgacc gatacggccg ttaaagaagt caaaacatct
     3361 gacaacgaag cacaagtcat tttagaaaac ggcaaaatcc tgaccgcccg cctgcttttg
     3421 gcggcagaca gccgtttttc gcaaacccgc cgacaactcg gcatttcttc agatatgcac
     3481 gactacagcc gaaccatgtt cgtctgccgc atgaaacata ctctttccaa ccaacatacc
     3541 gcttacgaat gcttccatta cgggcgcacc atcgcactgc tgccgctgga agaacacctg
     3601 accaataccg tcattaccgt cgataccgat aaaatcaaca gcgttcaaaa cctttcgccc
     3661 gaagaattgg cggcaagcgt aaaagaacag ctcaagggca ggctcggcga tatggaattg
     3721 gtcagcagca tccaccatta cccccttgtc ggtatgattg ccaaacgttt ctacggcaaa
     3781 cgcagcgcgc tgattggcga tgcggcggtc ggtatgcacc ctgtaacggc acacggcttc
     3841 aacttgggtc tttccagcgc agatattctg gcaaaactga tactcgaagc cgaacaacgc
     3901 ggtcaggaca tcggcgcatc ttccttgttg gaaaaataca gcaacaaaca tatgctgcac
     3961 gcccatccgc tttatcacgg aaccaatatg atgctgaaac tgtttaccaa cgaaaccgcg
     4021 ccggcaaaac tgctgcgcgg tttggtattg cgtgccggca acaacttccc gccgctgaaa
     4081 aagctgatta ccaaacaact gaccggttga aaactgccga aatgccgtct gaaacgtttc
     4141 agacggcatt tttg
//
LOCUS       DAWHXS010000133         4049 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWHXS010000133 DAWHXS010000000
VERSION     DAWHXS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.133"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(224..314)
                     /locus_tag="WIE22_001865"
     tRNA            complement(224..314)
                     /locus_tag="WIE22_001865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(278..280),aa:Ser,seq:gga)
     gene            complement(353..439)
                     /locus_tag="WIE22_001866"
     tRNA            complement(353..439)
                     /locus_tag="WIE22_001866"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(403..405),aa:Leu,seq:cag)
     gene            complement(475..561)
                     /locus_tag="WIE22_001867"
     tRNA            complement(475..561)
                     /locus_tag="WIE22_001867"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(525..527),aa:Leu,seq:cag)
     gene            650..3025
                     /gene="rnr"
                     /locus_tag="WIE22_001868"
     CDS             650..3025
                     /gene="rnr"
                     /locus_tag="WIE22_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ4127895.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRQ
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3364..>4049)
                     /locus_tag="WIE22_001869"
     CDS             complement(3364..>4049)
                     /locus_tag="WIE22_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ4127896.1"
                     /translation="VKWVKETYPHIQVIGGNIATAKAALDLVAAGADAVKVGIGPGSI
                     CTTRIVAGVGVPQLTAIHNVAEALKGTGVPLIADGGIRFSGDIAKALAAGAYSVMLGG
                     MFAGTEEAPGEIELYQGRSYKSYRGMGSLGAMSQGSADRYFQDKTDSTDKYVPEGIEG
                     RVPYKGPIVNIIHQLTGGLRSSMGYLGCANIAEMHEKAEFVEITSAGMSESHVHDVQI
                     TKEAPNYHR"
BASE COUNT          993 a         1031 c         1108 g          917 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt
       61 tgttaatcca ctataaaata taccgcaaaa cataccataa aaaaaacggt aacggatagt
      121 tgtaagcggt tgatgacgat tatagacaat aacgggtttt cccaatgaaa ttattgtttg
      181 aataataaaa aatcccaaac cgtaaaagtt tgggatttgt atttggcaga gaggaaggga
      241 ttcgaaccct cgatacgcta ttcacgtata cacgctttcc aggcgtgcga cttaaaccac
      301 tcatccacct ctctaatggc ggaaattatc ccaatcggga taatttatta tttggtgccc
      361 gggagaagac tcgaacttcc acacccatga ggataccagc acctgaagct ggcgcgtcta
      421 ccaattccgc cacccgggca atctaaatat tgaaataaag caaagcattt gatttggtgc
      481 ccgggagaag actcgaactt ccacacccgt gaggatacta gcacctgaag ctagcgcgtc
      541 taccaattcc gccacccggg ctttgcttta tttgctgttt tgcggtacag tgtcctgccg
      601 caaagaagtc gctattatat agttcataaa gagaatgtca acagtccaaa tgaataaaaa
      661 tattaaatct ttaaatttac gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta
      721 tgaacatcct ttgcccagtc gggaatggat aatcgaattg ttggagcgca aaggtgtgcc
      781 ttcaaaaatc gaatcgcttg cgcgcgagct gtcgattacg gaagacgagt atgtcttttt
      841 cgaacgccgt ctgaaggcga tggcgcggga cggtcaggtt ttaatcaacc gtcggggcgc
      901 ggtttgcgcg gcggacaaat tggatttggt caaatgccgc gtcgaggcgc ataaggacgg
      961 tttcggtttt gccgtgccgc tcacgcccgc caaagacggt gattttgttt tgtatgaacg
     1021 ccagatgcgc ggcattatgc acggcgatat tgtcactgtt cgtcctgccg gcatcgaccg
     1081 taggggccgc cgcgaaggga cagttctgga tattgtcgaa cgcgcgcaaa gcaaagtggt
     1141 cggccgtttc tatatggata ggggcgtggc gattttggag ccggaagaca agcgtctgaa
     1201 ccaaagcatc gtattggaac cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt
     1261 catcgtcggc gaaattgagg tttatcctga gcaaaaccgg caggcagtgg caaaaatcat
     1321 cgaagttttg ggcgattatg ccgacagcgg catggagatt gaaattgccg tgcgcaagca
     1381 tcatttgccg caccaattca gtgaagcgtg tgccaaagcc gtgaaaaaaa ttcccgacca
     1441 tgtacgcaaa agcgatttga aaggccgcgt cgatttgcgc gacctgcctt tggtaacgat
     1501 tgacggcgaa acggcgcgcg attttgacga tgcggtgttt gccgaaaaaa tcggacgcaa
     1561 ttaccgtctg gtcgtggcga ttgccgatgt cagccattat gtccgccccg atgacgctat
     1621 cgacacggac gctcaggaac gcagcaccag tgtttacttc ccgcgccgcg tgattcccat
     1681 gttgccggaa aacctgtcca acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg
     1741 tatggtgtgc gatatggtca tcacttacgc gggcaatatt aaagaatacc gcttctaccc
     1801 cgccgtgatg cgctctcatg cccgcctgac ctacaaccaa gtttggaaat ggctttcagg
     1861 cggcatcggg catccgttca aaacacaaat cgacacgctt tacaaactct tcaaaatcct
     1921 tcagaaaaag cgtttcgaac gcggggcggt ggagtttgac agcatcgaaa cccaaatgct
     1981 tttcgacgac aacggtaaaa ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa
     2041 gctgattgaa gaatgtatgc tggcggcgaa tgtttgcgcg gcggattttc tgttgaaaaa
     2101 caagcatacg gctttgttcc gcaaccattt gggccccacg cccgaaaaac tcgccaccct
     2161 gcgcgagcag ctcggtctgt tggggcttca acttggcggc ggcgacaacc cgtcgccgaa
     2221 agactatgcc gcgcttgtcg aacaattcaa aggcagacct gatgccgaat tgctgcaagt
     2281 catgatgttg cgctccatgc aacaggcggt ttacgaaccg cattgcgacg gacactttgg
     2341 tcttgcctac gaagcatacg cccacttcac ctcgcccatc cgccgctatc ccgacctgac
     2401 cgtacaccgc gccatcaaag ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg
     2461 gcaggctttg ggcgtgcata cctcgttctg tgagcgccgt gccgacgacg ccagccgcga
     2521 cgtggaaaac tggctgaaaa cctattatat gcgcgataag gtcggcgaag tattcgaagg
     2581 taaaatctcc ggcatgacca gttttggtat ctttgtaaca ctggacggca tccacattga
     2641 cggcttggtg catatcagcg atttgggcga agactatttc aacttccgcc ccgaaatcat
     2701 ggcaatcgaa ggcgaacgca gcggcatccg tttcaacatg ggggacaggg ttgccgtccg
     2761 ggtcgcccgt gccgatttgg atgacggaaa aatcgatttt gtcctgattg ccggggggag
     2821 cggcaggggg cggaaagtta aatcatccgc gtctgccaaa ccggcaggga cggcggggaa
     2881 agggaagccg aaaaccgccg ccgagaaaaa aacagcccga ggcggcaaag taaggggaag
     2941 gggcgcgtct gccgccgcag aatcggggaa aaaggcaaag aaaccggttc cgattaaggt
     3001 aaaaaaacgg aaaggcaaat cataatgctg acggggtggc ttggggaggg cggggcatac
     3061 tttcccgaag ccgccgcccg attttttgat atggcggccg gcgcatcatt tcaggccaat
     3121 cgaaacgccc gtattgaaag attgcgttta tttccaccgc cgttttaaag gccggcggta
     3181 ttcggcagac ggggcgcaaa cggcgttcag acggcatttt cattctttcg gcgtgtccgt
     3241 ccgaattgct ttgcccgtcc gcgcaatcag cccgtgcggc ttgccttgaa cggacaaaaa
     3301 atgccgtctg aaacccgaaa atcaggtttc agacggcatt ttgccttgaa aaggctgttc
     3361 aaatcagcgg tggtagttcg gtgcttcttt agtgatttga acgtcgtgaa cgtgcgattc
     3421 gctcatacct gcggaagtga tttccacaaa ttcggctttt tcgtgcattt cggcaatatt
     3481 ggcgcaaccc aagtaaccca tactggaacg cagaccgccg gtcagctggt ggatgatgtt
     3541 cacaatcggg cctttgtaag gaacgcggcc ttcgatgcct tcggggacgt atttgtcggt
     3601 gctgtcggtt ttgtcttgga agtagcggtc ggcagaacct tggctcatcg cgcccaatga
     3661 acccataccg cgataggatt tgtacgagcg gccttgatag agttcgattt cgcccggcgc
     3721 ttcttccgtg ccggcaaaca taccgccgag catgacgctg tacgcgcctg cggcgagggc
     3781 tttggcgatg tcgccggaga agcggatgcc gccgtcggca atcaggggaa cgcctgtgcc
     3841 tttgagggct tcggcaacgt tgtggatggc ggtcagttgc ggcacgccca cgcctgccac
     3901 gatacgggtg gtgcaaatcg atcccgggcc gataccgact ttgacggcat ccgcaccggc
     3961 ggcgaccaaa tccaaagcgg ctttggcagt ggcgatattg ccgccgatga cttggatgtg
     4021 cggataggtt tctttcaccc atttcacgc
//
LOCUS       DAWHXS010000134         4029 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWHXS010000134 DAWHXS010000000
VERSION     DAWHXS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.134"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            98..1172
                     /locus_tag="WIE22_001870"
                     /pseudo
     CDS             98..1172
                     /locus_tag="WIE22_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            1200..3625
                     /gene="hupB"
                     /locus_tag="WIE22_001871"
                     /pseudo
     CDS             1200..3625
                     /gene="hupB"
                     /locus_tag="WIE22_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
BASE COUNT         1094 a         1266 c          895 g          774 t
ORIGIN      
        1 cgatttttgc accgacccac aaaaccagtt gacatcatat aacatttaaa ctgataatca
       61 aaactattat taacacatca tcaagattga gtatgcgatg aaatacaaag ccctgtcctt
      121 actgccgctt gtcgctgccc ttgccgcctg tgcggggggt agccgaaccg cacgtccccg
      181 tgtccatccc caccgccacg ccgctgccca aaggcgaggt aacgttatca agcgataacg
      241 gcaatatcga aaacatcaac accgccaaca ctgaaacaca tccgccgcgc cgcaccagac
      301 gctccctcta tgcctccccg caaaacacat cctccggtat ttccattcag cagcgggaag
      361 tagaaaaaat ctatttcggt gttaaatcat cagaaaaatc attcatcttc caaacacccg
      421 gcggcgcgca atacgccctt tcatcctacg ccgaccccat cgtccccagc tactcctccc
      481 ccgatttcaa aattcccgac cgccacgcag ggcagcggct tgcagacggc agccgcatct
      541 ttatctgctg cagcgactcc ggggcaacca gagaagcgga aattaccaaa caagattata
      601 tgaaattcgg ggcatggata ggtcccaacg gcgaaatcga cctcttcgcc ggcggcttcc
      661 ccgtcggcaa aacaccgaca tcaagcagtt attacggcag cagcacgccc gaaacccaag
      721 gcaaaggcaa aatcacttat caggtttggg gcatccgcgt caaagacggg caatttgtta
      781 cctcctctta tacgccgccc aaaggcagct cttttaccgg ctacaccaac acccccgtcc
      841 tttcctttat caccgccaat ttcaacagca acaaactggc aggcgaaatc cgcggcaaca
      901 gcgattacgg accgagtgtg aaaattgaaa acgcaacgat cagcggtccg agcttttccg
      961 gcaatgccac ctccggcggc aaaaccggca acttggaagg caagtttttt ggaaaattca
     1021 acggcagcta cggcaacact gaaaccagca taggcggcaa aatcactttt aaagacgacc
     1081 gctccctcga taccgtattc ggcggtgtga gttatgtaaa aaaacttgat gaaaccgcca
     1141 atcgagatac cgaacacctt actaaacaat aaaccatctc atccctacaa aggtttctta
     1201 tgtccatccc cttcaaaccc gtattggctg ccgccgccat cgcccaagcg tttcccgcct
     1261 ttgcggcaga ccccgcgccg cagtccgccc aaacgctgaa cgaaatcacc gttaccggca
     1321 cgcacaaaac ccaaaaactc ggcgaagaaa aaatccgccg caaaacctta gacaagctct
     1381 tggtcaacga cgaacacgac ctggtgcgct acgaccccgg catttccgtc gtcgaaggcg
     1441 gcagggcggg ttctaacggc tttaccatac gcggcgtgga caaagaccgc gtcgccatca
     1501 acgttgacgg gctggcgcag gcggaaagcc gctcttccga agccttccaa gaattgttcg
     1561 gcgcgtacgg caacttcaac gccaaccgca acacttccga gccggaaaac ttttccgaag
     1621 taaccatcac caaaggcgcg gactcgctca aatccggcag cggcgcattg ggcggcgcag
     1681 tcaattacca aaccaaatcc gcaagcgatt atgtttccga agacaagccc taccatttgg
     1741 ggataaaggg cggcagcgtc ggcaaaaaca gccaaaagtt cagcagcatc accgccgccg
     1801 gcaggctctt tggtttggat gccttattgg tttatacccg ccgcttcggc aaagaaacca
     1861 aaaaccgctc gaccgagggc gatgtcaaaa ttgaaaacaa gggatatgtt ttcagcccaa
     1921 cccaatcatt cagcaagtac cagacctacg tagccacagg ggttgcgcgc tcccaacccg
     1981 acccgcaaga atgggtaaac aaaagcacct tgttcaagct gggctacaat ttcaacgacc
     2041 gcaaccgcat cggctggatt tttgaagact cccgcaccga ccgttttacc aacgagctgt
     2101 ctaatttgtg gacgggtaca atttactctc ccgcaacggg cgactaccgc caccgccaag
     2161 acgtgagcta ccgccgccgc tccggcgtcg aatacaaaaa cgaattggaa cacggcccgt
     2221 gggacagcct caagctgcgc tacgacaagc agcgcatcga tatgaacact tggacttggg
     2281 acatcccgaa aaattacgat ttgcgcggca tcaacagtga agtttaccat tcgttccggc
     2341 atatccgcca aaacaccgcg caatggactg ccgattttga aaaacaactc gacttttcca
     2401 aagccgtttg ggcggcgcaa tacggcttgg gcggcggcag aggggacaat gccaactcgg
     2461 attacagcta tttcgtaaaa ctgtacgacc ccaaaatctt cacttccaac caagccaaaa
     2521 tcacgatgct gatcgaaagc cggtcgaaat ataaatttgc ctattggaac aatgcgtttc
     2581 acttgggcgg caacgaccgc ttccgcctga atgcgggcta cgctacgaca aaaacagcag
     2641 cagcgcgaaa gacgatccga aatacaccac cgccatccgg gggcagattc cccatttggg
     2701 ttcggaacgc gcgcacgcgg gcttcagcta cggcacgggg ttcgactggc ggtttaccaa
     2761 gcatctgcac ttgttggcaa aatacagcac cggcttccgc gcaccgactt cggacgaaac
     2821 ctggctattg ttcccgcatc ctgactttta ccttaaagca aaccccaacc tgaaggcaga
     2881 aaccgccaaa aacttcgagc tgggacttgc cggcagcggc aaagcaggaa actttaaatt
     2941 ctccggtttc caaacccgct accgtaattt tatcgaactg acctacatgg gcgtatcctc
     3001 agacaagcct aacagcccta attatgcgcc gatttccgac ggtactgcat tggtcagctc
     3061 gcccgtttgg caaaaccaaa accgctcctc ggcatgggtg aaaggtttgg aatttaacgg
     3121 cacatggaac ctcgacagca tcggtttgcc gcaaggcacg cacgcaggtg tcaatgtcag
     3181 ctacatcaaa ggcaaggcca aacaaaccaa cggacaggaa acccctatta acgccctttc
     3241 gccatggagt gcggtttaca atctgggcta cgacgctcct tccaaacgtt ggggcatcaa
     3301 cgcctacgcc acgcgcaccg ccgccaaaaa gccgtccgac accgtccaca gcaacgacga
     3361 cttgaacaac ccgtggcctt atgccaaaca cagcaaggcc tatacgctgt tcgacctttc
     3421 cgcctacctc aacatcggca aacaggttac gctccgcgcc gccgcgtaca acattaccaa
     3481 caagcagtac tacacttggg aatctttacg cagcatccgc gagttcggca cggtcaaccg
     3541 cgttgacaac aaaacccacg ccggcatcca acgctttacc tcgccgggca ggagctacaa
     3601 tttcaccatc gaagcgaagt tctaagctcc tgatgtgcca atgccgtctg aaacccgatg
     3661 atttgggttt cagacggcat tttttacggt cgcaccgccg ccccctttca ggcttgtacc
     3721 aagcccaatc ccgcaactct ccgtcattcc cgcaactctc cgtcattccc gcaactctcc
     3781 gtcattcccg caactctccg tcattcccgc aactctccgt cattcccgcg actctccgtc
     3841 attcccacgg aagtgggaat ctagaaacgc aaagctgcaa gaatttatcg gaaatgactg
     3901 aaactcaacg aacctggatt cccgcctgcg cgggaatgac gaagctatcc gcacagaaac
     3961 ctgcaccacg tcattcccac gaaagtggga atccagaacg taaaatctga agaaaccgtt
     4021 ttatccgat
//
LOCUS       DAWHXS010000135         4026 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWHXS010000135 DAWHXS010000000
VERSION     DAWHXS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.135"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1178..1615)
                     /gene="rnhA"
                     /locus_tag="WIE22_001872"
     CDS             complement(1178..1615)
                     /gene="rnhA"
                     /locus_tag="WIE22_001872"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ4127897.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1729..2598
                     /gene="tehB"
                     /locus_tag="WIE22_001873"
     CDS             1729..2598
                     /gene="tehB"
                     /locus_tag="WIE22_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ4127898.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAESLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPEVIADMQAHTAVG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVKYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2648..3454)
                     /gene="thiD"
                     /locus_tag="WIE22_001874"
     CDS             complement(2648..3454)
                     /gene="thiD"
                     /locus_tag="WIE22_001874"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ4127899.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETIFAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          875 a          985 c         1110 g         1056 t
ORIGIN      
        1 aatttaatcc actatagttt aatttgtctg tccgcattgc cgctaattgt accacttcat
       61 cgtatttcct tttcggttga aaccccgcca ctcggacatc tgtccttcgg ggcggcagga
      121 tcagacttta tttgggaggg ggtgtaaccc cttccaaatc aggacgacac atagggaggt
      181 gctttatgtg tcgtcctgtg tgttgaaata tatcggacgc gtttgaacag ttgtgcggca
      241 aactgtttta gcggcaggca agagatgccg tctgatgccg tctgaagccc attgtccgtc
      301 attcccacac aggcagttgc tacggttgct gaagcccctt agccgtcgcg caggcggttg
      361 ctacggttac taaaatcccg tcattcccgc gcaggcggga atccagtccg ttcggtttca
      421 gttgtttctg ataaatcttg cagcgttggg tttttagatt cccgctttcg cgggaatgac
      481 ggggcggtcg gatgccgcct gaaactccgt cattcttgca acttttcgtc attcccgtga
      541 aaacgggaat ctagaacttc aaactttcag ataatctttg aatattgccg ttgcccgacg
      601 gtctagattc ccgcttgcgc gggaatgacg gtagagcggt ttcagttgct cccgataaat
      661 gccgcaatct caaatcccgt cattccctca aaaacagaaa aacaaaatca gaaacctaaa
      721 atcccgtcat tcccgcagct cttcgtcatt cccgtgaaag cgggaatcta gaccttttaa
      781 attttcacac ggcttttgaa tattgccgtt acccgaaggt ctggattccc gcctgcacgg
      841 gaatgacggc agagcggttt cagttgtttc taaataaatt tttgcagcgt tggattttta
      901 gattcccgct ttcgcgggaa tgacggggcg gtcggatgcc gcctgaagcc cgacaaagcc
      961 cccaatgccg tctgaaacct cgtcattcct ctgctgtccg caacttttcg tcattcccgc
     1021 aaaagcggga atctagagcc tctaaatttt cagacggctt ttgaatattg ccgttgcccg
     1081 acggtttgga ttcccgcctg cgcgggaatg acggtggagg acgatgccgt ctaaagccca
     1141 ttagcggttt cagacggcat ttttgccgga gcggcagtca ggaaaactgc gctgcgccac
     1201 ggtttgccaa atcgtcggcg cgttcgtttt ccgcgtgtcc cgcgtgtcct ttaacccaag
     1261 tccagctgac ttggtgctgc ccaaccagaa cgtcgagttc tttccacaag tcgtcgtttt
     1321 tgacgggctg tttggcggcg gttttccagc cgttgcgctt ccaaccgtgt atccagtttt
     1381 ccatgccgtt tttgacgtat tgcgagtcgg tgcagatgat gacggtgcag cggcgtttga
     1441 gcgatttcag tccttcgatg acggcggtca gctccatgcg gttgttggtg gtttgcgctt
     1501 cgccgccgaa aagttctttt tcgtggctgc cgtagcgcat caacacgccc cagccgcccg
     1561 cgccgggatt gcctttgcac gcgccgtcgg tgtaaaggta aacggtttgg tccattattg
     1621 tgcctttgtg tggtgtccgg cgtgattata agcggtttgt gcgccgtgcc gtctgaaaga
     1681 ttgcggcgga ttaaaatagg catataatgt gcaactttat ttttgaatat gaaggagcgg
     1741 atagtgggtc aaagcggcga gttgttttgt ttcggacaaa tgcccgtatg gaaggcggaa
     1801 aacctgccgg aaattttgtt gtcgggttat tcgtccgaag gaggggaatg ggtctgcctg
     1861 aatgtgttgc agggcgatgt cgaagtccgc acgcaggacg gggcggcaga ggtttggtcg
     1921 gcggaaagcg gcgattgcgt gtttgcgccg cagcaggtgt tttcggtcaa actgaaaacg
     1981 gacgatgccg aaatccgttt gtcgctgtat tgcgcggcgg cagactattt tcacaaaaaa
     2041 tacgggatga gtgccacgca ttctgcggtc gcggcggcac gggataccgt accggcgggc
     2101 agggcgttgg atatgggctg cggacagggg cgcaacgcgc tgtttctcgg tttgaagggg
     2161 tttgaagtta ctgcagtcga tcacaatccc gccgccttgg caaacgtggc ggagctggca
     2221 gaggcggaga gtttgaacgt ccgcacgctg gaatatgatt tgaacgccgc cgccttgcag
     2281 ggcgagtttg attatattgt ggcaacggtg gtgctgatgt tcctgatgcc gcagcgcgtg
     2341 cctgaagtga ttgccgatat gcaggcgcat acggcggtgg gcgggtacaa cttaatcgta
     2401 tcggcaatgg atacggcgga tttcccctgt ccgatgccgt tccccttcaa atttaaagag
     2461 ggagagctga aggattatta tcgggattgg gagctggtca aatataaaga agaattgggc
     2521 gcgatgcacg ccaaagacga aaacggcaat ccgatacggt ttaaatttgt aacgatgctg
     2581 gcgaaaaagc ctgaataatc ctcaaaaatg ccgtctgaac attttgtttc agacggcatt
     2641 tttacgtcta gtcccgatac gcccaatgat tgaccggccc gtgtcctgcg ccgatttcca
     2701 aagggtttga gattgccgcc gtgatgtatg ccttggcagt ctgtacggct ttgcaaacgt
     2761 ctaagccttt tgccaactcg gcggtaatgc aggcagaaaa cgtgcagccc gtgccgtgcg
     2821 tgtgggcggt tggaaagcgc gggctgtcca attccagcgt ttcattttgc gtaaacagcc
     2881 aatccgtgca gcgtccgctt gtgctgccgt tcaaatgtcc gcctttgata acgacatttt
     2941 tgacaccgta atcaagcagg atttttgccg cacgttccgc atctttacgg ttttcgatat
     3001 gcacgccggt cagggcttcc gcctcgggca ggttgggggt caatacatcc gtatcgggaa
     3061 gcagcaggcg cgtcagtgcc gcaacggcgg aatcctgcaa cagcggcgca ccgcctttgg
     3121 caatcatcac ggggtcgagt acgcgcctgc cgaagtcgca gtgtttcagt ttgtcggcaa
     3181 cgcattcgat gatttccgcc gtgccgagca ttcctatttt gtaggcgcgg atgtcaaagt
     3241 cttccctgat tgcttggatt tgtgcgaaga tggtttcggt cgggacgaga tgaaccgccg
     3301 acacgcccaa ggtattttgc gcggtaacgg cggtgatgac gcacgttccg aacacgccgc
     3361 gcatctgaaa cgttttcaaa tccgcctgaa tgcccgcacc gccgcccgaa tccgaaccgg
     3421 cgatggtaag cgtttgcaca aaagagcttt tcatttgttc ctccttaata ttgtgtccga
     3481 taaaaagggc taatgcctgt aaaaaagcac ggcgatgccg tctgaaaacc gcagcgcggg
     3541 gtttgtggtg cattatatgc cgtattgcag gacaaggcac agggcagggc ggcggggtgc
     3601 atcgtagcaa ctgtattttt gcccgacggg gtgaaaaata cagttgctac acccactgat
     3661 aatgaaaaaa gagagcaatg ccgtctgaac cgcgtgttgc ggcgagcata aaccggcacg
     3721 ctttattcgt cattcccaca aaaacagaaa cctaaaattc cgtcattcct acgaaagcgg
     3781 gaatccagtg tgttcggttt cagttgtttt tgggtttcgg gtgatttcca aatcatcatt
     3841 cccgcgcaga tgatatgttg cccgtcaaca caaaataaaa aacaaagttg caatatactg
     3901 atttatattg ttatttttat ttacgtttat ttacgatatg caaatgcacg gttacacaaa
     3961 tatattcgcg taaccgttta attttgttga attttattga ttcaatcggt gtctttccgc
     4021 atcgta
//
LOCUS       DAWHXS010000136         4000 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWHXS010000136 DAWHXS010000000
VERSION     DAWHXS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.136"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(64..1314)
                     /locus_tag="WIE22_001875"
     CDS             complement(64..1314)
                     /locus_tag="WIE22_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ4127900.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSAIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYTVYGA"
     gene            complement(1381..1576)
                     /locus_tag="WIE22_001876"
                     /pseudo
     CDS             complement(1381..1576)
                     /locus_tag="WIE22_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1795..3570)
                     /gene="ggt"
                     /locus_tag="WIE22_001877"
     CDS             complement(1795..3570)
                     /gene="ggt"
                     /locus_tag="WIE22_001877"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ4127901.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3738..4000)
                     /locus_tag="WIE22_001878"
                     /pseudo
     CDS             complement(3738..4000)
                     /locus_tag="WIE22_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin, Grx4 family"
BASE COUNT          997 a          951 c          943 g         1109 t
ORIGIN      
        1 gcctgaaaca ttgtgagatt tcaggcggca ttggattgct tggcggaata tttttaaaaa
       61 ggcttacgcg ccgtaaacgg tgtatttgtc gcagagggcg gtaatctgtt tgcggacgtt
      121 ttccagattc gcttcgtctt cggggttaga caatacgtcg gcaaccaagt tcgccaatac
      181 gcgcgcgtcg gcttcgttaa aaccgcgtgt ggtcatggca gcggagccga tgcggatgcc
      241 ggaggtaacg aagggttttt ccggatcgtt cgggatggcg tttttgttga cggtgatgtg
      301 cgctttgccc aaagcggctt cggcggcttt accggtgatt ttcatcggtt gcaggtcgac
      361 gaggaaaacg tggctttcgg tgcggccgga aacgatgcgc aaaccgcgtt taaccaactc
      421 ttctgccatg gcggcagcat tgattttcac ttgttttgcg tattgtttga actcgggttg
      481 caacgcttct ttaaacgcta cggctttggc ggcgatgacg tgcatcagtg gaccgccttg
      541 taggcttggg aagatggcgg agtttaacgc tttttcgtgg gtattgtcgc ggcacaaaat
      601 cacgccgccg cgagggccgc gtagggtttt gtgggtggtg gtggtcacga agtcgcagaa
      661 tggcacgggg ttgggatatt cgccaccggc aatcagtccg gcatagtgcg ccatgtcgac
      721 aaagaggtat gcgccgactt tatcggcgat ctcgcggaat tttgcccagt cgatttgcaa
      781 cgcgtaggca gacgcaccgg ccacaatcat tttaggtttg tgttcgagag ccaagcgttc
      841 cacttcggca taatcgagca cttcgttttc atccaaacca taagtaacgg cattgtagag
      901 tttgccggaa atattaacgc tcgcgccgtg ggtcaggtgg ccgccgtgcg ccaaagacat
      961 acccaaaatg gtgtcgcccg gttttaaaac ggaagcgtac acggcttggt tggcttgcga
     1021 gccggagtgc ggttgaacgt tggcgtattg cgcgccgaac agttttttga cgcggtcgat
     1081 tgccaattgt tcgacaatat cgacgtattc gcagccgccg tagtagcgtt tgccggggta
     1141 gccttcggcg tatttgttgg tcagctggga accttgcgct tccattacgg cgcagctgac
     1201 gtagttttca gaggcaatca gctcgacgtg gtcttgctgg cgttggtctt cttgggcaat
     1261 ggctgctgcc aaatcggggt catattgtgc gagggtaacg ctttttgaaa acatgttctc
     1321 ggctcctttg tgtaatcagg gtatcatgag tgttttttgt ataaaaaaat acttcaaaac
     1381 ctaaggcaga tagtccataa tgcgtaaatt ttctttggca ttatcaggta atttatttaa
     1441 catgctggtt tttagcgtct cattaccttt atgttagtac aaggctaccc aaaggcaata
     1501 catcaaatgg taaattttcg gcagtttgtg caaaatcaat caaataacca gcaccaacca
     1561 catctttatc agccataacc cgctgataca ccacttgcca agacaactct cctgccccac
     1621 catgtcttaa aatcagatga atggcttgta atgctgtgtt tggcgtctca caaattgcga
     1681 agatctgctc agaagcttgc ttgatggctt gtatttttgt ttgattactc acaatttccc
     1741 cctattttaa tattaactta atgcggtcaa attcacaaaa tacaagcttc acctctaatc
     1801 acccatcact cgaccttctc ggcgtggatc ggcaccacca accagcctgc ttggctcgat
     1861 aataatggct tgaacacctg aatttagctc acgcacgtca gtcttatagc ccaaatcatt
     1921 taatgcttgt tgccactgga gggcggttgt acccgtttct agttcatagt taccaaagcg
     1981 atttaataaa ttgggtgcac tgatggcatc ttggatatcc atattccagt cactatgtgc
     2041 cacaatcgtc ttagcgacat agccaatgat acggctacca cctggggagc cgattgccat
     2101 ataaggcttg cctgctttaa atacgatggt tggtgccatt gaggagcgtg gtctcttgcc
     2161 gggctcgaca cgattggcga cctgtttgcc ctgctttatt ggctcaaaac taaagtctgt
     2221 cagctcatta ttcagcaggt agccatttgc catcaaagtt gagccaaacg cattttcaat
     2281 ggaagtcgtc attgatagca cattgcccgc cttatccaca attgatatat gactggtaga
     2341 aggtaactca atcgcttgtg aggacaccca ctcatgaata aaatcgcctg cagatacgct
     2401 aggcaatgcc ttatccgact gctcaagcag ctggctgcga tgttttaggt agtctttaga
     2461 aatcaactgg cgaatgggta ctggtacaaa atcagggtcg cccaaatata catcacgatc
     2521 cgcaaacgca agcctagaag cgtcgcccaa gagacgtaaa ccttcagcat cataccccac
     2581 ctgattgggt gaaaattcat ttaaaatccc caaaatttga cccacagcaa tcccacctga
     2641 gcttggtgca cccataccgc atacttcata aatacgataa gtcacacaaa caggcgggcg
     2701 ttccaccact tgataatcag ataaatcttg taaggataat tgaccggggt tatccttagc
     2761 attttggaca actgaaacga tattttgggc atatttacca gtatgcagag cttttgcacc
     2821 ttgagctgct aacgcctgaa cactgtcagc aaattctaaa tttttcagca agctgcctgc
     2881 ttgtagcggc acaccattcg gcaaaaaata agcggctgtt tttggatagc gtgccaaatg
     2941 ctgctgattt tgctcaaccg agatggcaag ccttggcgac acctcaaagc cttgttttgc
     3001 caagcggatc ggtgtatcaa ataattttcc ccaaggcaat acaccgtatc gctgatgtat
     3061 tgtctccatc agtttaggga tagcaggcgt acccaccgag cgaccaccga ccaccgcttc
     3121 cataaatttc aatggttgac catctttatc caaaaataat tccggcgtcg cacgcatcgg
     3181 tgccgtctca cgcccatcaa atgtggtcaa tgttttggcg gtattatccc aatacaacac
     3241 aaatgcacca ccgcccaagc ctgacgactg tggctctacc aagcttagtg tcgtctgcac
     3301 cgccaccatc gcatctgcag cgctaccgcc ttgctttaag atatcatagc cagcttgtgt
     3361 tgctaatgga ttggctgacg ctaccataaa atcacttgca atcacctgct tttgttcgat
     3421 cagtcccgtt gcatgttcag gcgtgtgagc gtctgcacct gtgatgacag cagaatgagt
     3481 attaacctta ccttgattgg catggatgac ttgacatcca gagattgtca tagacattat
     3541 caatgcagtc aataaatatg ttttagccac aagcactcct tcgcctgagt ttgattgatg
     3601 attcatacaa ggcatgctga ttattgtatt taatatggct aaataattca atccaaacta
     3661 tcaatcttga ccatcaaaaa aagaccgcta atgtcatcag cagtcttttt tgatatttat
     3721 tttaagatat taagtaatca gacctttggg ctatgctctt caatgagtgg ttttagctca
     3781 cctgattggt acatttgtag gataatatca ctaccaccga ttaactcacc attaacccaa
     3841 agctgtggaa aggttggccg attggcgatg agtggtagag tactgcgaat ttctgggttt
     3901 tctaggatat taacaaaagc aaagggtctg ccaattgggt cagcacctct actgcacgcg
     3961 ctgaaaatcc acattgggga aactggggcg tgcctttcat
//
LOCUS       DAWHXS010000137         3977 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWHXS010000137 DAWHXS010000000
VERSION     DAWHXS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.137"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..601)
                     /locus_tag="WIE22_001879"
     CDS             complement(<1..601)
                     /locus_tag="WIE22_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ4127902.1"
                     /translation="MGRHFGRRRFLTAAAVAAAGAAVSFLPNPFAAGGEKRNGDEKRD
                     ENVFFWKGVALGSGAELRLFGVDNRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPSADFLELLSLCRDIHALTDGAFDPTVQALWKLYADYFAARPDAETPPPQN
                     SVDEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQGYS"
     gene            complement(610..1404)
                     /locus_tag="WIE22_001880"
     CDS             complement(610..1404)
                     /locus_tag="WIE22_001880"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ4127903.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1827..3161
                     /gene="gdhA"
                     /locus_tag="WIE22_001881"
     CDS             1827..3161
                     /gene="gdhA"
                     /locus_tag="WIE22_001881"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ4127904.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3303..3677
                     /locus_tag="WIE22_001882"
     CDS             3303..3677
                     /locus_tag="WIE22_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127905.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3721..>3977
                     /locus_tag="WIE22_001883"
     CDS             3721..>3977
                     /locus_tag="WIE22_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ4127906.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
BASE COUNT         1072 a         1049 c          944 g          912 t
ORIGIN      
        1 cactatagcc ttgcgcgatg ccgttcaagg acaaacccat acccttttcg gcaaaacgga
       61 tttcacggtc gtcaaacgag actttgccga agccgacccg tttcagggct tcgtccacgc
      121 tgttttgagg aggcggcgtt tccgcatcgg gacgggcggc aaaataatcg gcatacagtt
      181 tccacaacgc ctgcactgtc ggatcgaacg cgccgtccgt cagcgcgtga atatcgcggc
      241 acaggctcaa cagttccaaa aaatccgccg acggcgaagt cagataaccg tccctgttca
      301 ggcggctgat caggctgtct tcacggtaaa ggctgaacat tttttccaaa cgcgccactt
      361 ccgccaaaac cttgttgacc aaatccgccg cctgtctgtt atccacgccg aacaggcgca
      421 gctccgcgcc ggaacccagc gcgacacctt tccagaaaaa cacattttca tcgcgttttt
      481 catccccgtt gcgtttttcg ccgccggcgg caaaaggatt cggcaaaaaa gaaaccgccg
      541 cgcccgccgc cgcaacggcg gcagccgtca aaaaacgcct gcgcccgaaa tgcctgccca
      601 taccgcctcc taaaccgaca ctgccgcctt gatgtgcgga tgggggtcgt agccttccaa
      661 ctcgaaatct tcaaacttga aggaaaacaa atctttgact tcaggattga ttttcatcac
      721 tggcaaggcg cgcggttcgc gttccaactg caatgcggcc tgctcgaaat ggttgcggta
      781 caaatgcgcg tcgccaaacg tatggacaaa ctcgcccgcc tccagcccgc acacttgcgc
      841 catcatcatc gtcaacagcg cgtagctggc aatattaaac ggcacaccaa ggaaaatatc
      901 tgcactacgc tggtaaagct ggcaggacag tttgccgtcg gcaacgtaaa actgaaacag
      961 cgcgtggcag ggcggcaagg ccatttcatc gaccaaagcc ggattccacg ccgatacaat
     1021 caggcggcgc gagtcgggat tcttcttgat ttgttccagc acattggcga tttggtcgat
     1081 atgcctgccg tcgggcgcgg gccagttacg ccactggtag ccgtaaaccg ggcccaagtc
     1141 gccgttctcg tccgcccact cgtcccaaat ggaaacattg ttgtccttca ggtatttgat
     1201 attggtatcg cctttgagaa accaaagcag ctcgtggata atcgagcgca aatgcagctt
     1261 tttggtcgtc agcagcggaa aacctttgcc caagtcaaaa cgcatctgat aaccgaatac
     1321 ggagcgcgtg cccgtaccgg tgcggtctga tttgtccgta ccgttgtcga ggacgtggcg
     1381 catcaagtcc aaataggcct tcatcatcat ctttcatcaa attaaacggc gcatattgta
     1441 acatttccgg ccaacgcccg agtccggaca aacgcacacg ccgccccgcc ggacgataaa
     1501 aacgcagata cgggcaggca ggattgttgg caatttcagt ccttttccac agtaaaaccc
     1561 ggtgggaaaa caaaattacc ttgattggaa tcaaaaaatc tagtttaatt acttagaata
     1621 aaatttcaat aatattttta tttacgaaat taatttatga ttattgattg attatcggca
     1681 acaaacatca aacatcgaaa atatggaaaa aataatgtca acaatttttg ccaaatcggg
     1741 cttggcatca gaaaaaaata ggtttatatt cccacctaca aatttgtttt cccattagta
     1801 cactatcaac caaaaggagt atccgaatga ctgacctgaa caccctgttt gccaacctca
     1861 aacaacgcaa tcccaatcag gagccgttcc atcaggcggt tgaagaagtc ttcatgagtc
     1921 tcgatccgtt tttggcaaaa aatccgaaat acacccagca aagcctgctg gaacgcatcg
     1981 tcgaacccga acgcgtcgtc atgttccgcg taacctggca ggacgataaa gggcaagtcc
     2041 aagtcaaccg gggctaccgc gtgcaaatga gttccgccat cggtccttac aaaggcggcc
     2101 tgcgcttcca tccgaccgtc gatttaggcg tattgaaatt cctcgctttt gaacaagtgt
     2161 tcaaaaacgc cttgaccacc ctgcctatgg gcggcggcaa aggcggttcc gacttcgacc
     2221 ccaaaggcaa atccgatgcc gaagtaatgc gcttctgcca agcctttatg accgaactct
     2281 accgccacat cggcgcggac accgatgttc cggccggcga catcggtgta ggcgggcgcg
     2341 aaatcggcta cctgttcgga caatacaaaa aaatccgcaa cgagttttct tccgtcctga
     2401 ccggcaaagg tttggaatgg ggcggcagcc tcatccgtcc cgaagcgacc ggctacggct
     2461 gcgtctattt cgcccaagcg atgctgcaaa cccgcaacga tagttttgaa ggcaaacgcg
     2521 tcctgatttc cggttccggc aacgtggcgc aatacgccgc cgaaaaagcc atccaactgg
     2581 gcgcgaaagt gctgaccgtt tccgactcca acggcttcgt cctcttcccc gacagcggta
     2641 tgaccgaagc gcaactcgcc gccttgatcg aattgaaaga agtccgccgc gaacgcgttg
     2701 ccacctacgc caaagagcaa ggtctgcaat actttgaaaa ccaaaaaccg tggggcgtcg
     2761 ccgccgaaat cgccctgccc tgcgcgaccc agaacgaatt ggacgaagaa gccgccaaaa
     2821 ccctgttggc aaacggctgc tacgtcgttg ccgaaggtgc gaatatgccg tcgactttgg
     2881 gcgcggtcga gcaatttatc aaagccggca tcctctacgc cccgggcaaa gcctccaatg
     2941 ccggcggcgt agcaacttca ggtttggaaa tgagccaaaa cgccatccgc ctgtcttgga
     3001 ctcgtgaaga agtcgaccaa cgcctgttcg gcatcatgca aagcatccac gaatcctgtc
     3061 tgaaatacgg caaagtcggc gacacagtaa actacgtcaa tggtgcgaac attgccggtt
     3121 tcgtcaaagt tgccgatgcg atgctggcgc aaggcttcta agcaaacgcc gccgctccgc
     3181 aaacaaaatg ccgtccgaac cgcaaatgct gttcagacgg catttcatta tccgcccgtt
     3241 cgaatcgggt gatactattt gtatccagaa ttgttggata gtttgggagt ggagaggaag
     3301 aaatgattat ggaccaatac tatatggagt taaagaataa gttatctaac agaccaatcc
     3361 tactggataa tactaatgat tttctttttg ttttagtaaa tacagtgaaa gctatgatag
     3421 aaaatactga taaaagtcag ctttcagagt tagataaaat tttagacggt gtcacaagtc
     3481 aagaattgaa acttgcctat gatttttgtc aagggaaatt cggtcaagca ggtttttctt
     3541 atcgtaggca cccgaattat ttttatttgt ccagtttaat tgcgactttt ccagaatttg
     3601 agttgtctaa agcagaccga gattacctca aaggaatcat aaattttgat aactaccttt
     3661 tatatgagtt agattagtct ttaatcacct caaaacccta tttattcaaa aggagaaaaa
     3721 atgattgagc tgaaattagt agatgagagc agttttcagg cagtgctgga tttgaaaata
     3781 tctgaagctg atgaacgagc acgttttgtg gctccaaatg tacgctcttt agctgacgca
     3841 tggctctacc gggaaaatga agatgtgttt ccgagggcaa tctattggga taagcaagtg
     3901 gttggctttc tcctgctgga aatagacaag gatgaagcag aatactttat ctggcggata
     3961 atgattggtc agcagtg
//
LOCUS       DAWHXS010000138         3965 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWHXS010000138 DAWHXS010000000
VERSION     DAWHXS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.138"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(256..681)
                     /locus_tag="WIE22_001884"
                     /pseudo
     CDS             complement(256..681)
                     /locus_tag="WIE22_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            824..1276
                     /gene="dut"
                     /locus_tag="WIE22_001885"
     CDS             824..1276
                     /gene="dut"
                     /locus_tag="WIE22_001885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ4127907.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1353..2540
                     /gene="dapC"
                     /locus_tag="WIE22_001886"
     CDS             1353..2540
                     /gene="dapC"
                     /locus_tag="WIE22_001886"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ4127908.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            2802..3581
                     /gene="yaaA"
                     /locus_tag="WIE22_001887"
     CDS             2802..3581
                     /gene="yaaA"
                     /locus_tag="WIE22_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ4127909.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTPFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRAQLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3664..3754
                     /locus_tag="WIE22_001888"
     tRNA            3664..3754
                     /locus_tag="WIE22_001888"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3698..3700,aa:Ser,seq:tga)
     gene            3832..3921
                     /locus_tag="WIE22_001889"
     tRNA            3832..3921
                     /locus_tag="WIE22_001889"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3866..3868,aa:Ser,seq:cga)
BASE COUNT         1040 a          940 c          975 g         1010 t
ORIGIN      
        1 cccgtcattc ccacgaaagt gggaatctag aaatttaatg ttgcggcact agccaaaaaa
       61 accgaaaccg aacggactag attcccgcct gcgcgggaat gacgattcat atagtggatt
      121 aacaaaaatc aggacaaggc ggcgaagctg cagacagtac agatagtacg gaaccgattc
      181 acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgctgt
      241 actggttttt gttaatccac tataaaaagg catattgaat gcgggcaaac cggctgcttt
      301 ccgtttttgg atttcggaga atgccatcgc ccagctttca tcacacataa aaaacagtgc
      361 gggcacggct tttttcagcg gtatttcttt caggtgcggg gcaagtgccg cccccatcag
      421 gatatgccga gaattaatca taaaggttac ggtggcgata agcagtatcg gcagaggttc
      481 cgcccacagg ttgaccgtgg caaactcgga gccgccggcg aagttcatac cggtcatcaa
      541 caacatttcc agccagctca tgcctttttg tccaccctgc ataccgagta ttaatgccca
      601 aggcaaaagc ccgatcagca tgggcgaact ttctttgatg ccgcgtataa attcgttacg
      661 gggcgagctt atgtgtgtca tattaatatt gatatgtaag aaggtcgggc attatacaca
      721 gacggagtat ttcaaaacaa aacctatttg ctgtgtttca gcgtaaacac ggcttgtgta
      781 taatctccca tctttgaaac cggccgtatg caggagcaag acgatgaata ttgaagtaga
      841 aatgaaagta ttggacgaac ggatggcgga ttttatccct gcctatgcaa cggagggttc
      901 tgcaggttta gatttgcgtg cctgtttgga tgaggaagtc gttttgcagc cgggtgaaac
      961 gtttcttgtg ccgacaggtt tggcaattta tttggcgaat cccgcatatg ccgccgtttt
     1021 gctgccccgt tccggcttag ggcataaaca cggcatcgtt ttgggcaatt tggtcggttt
     1081 gatcgactcc gattatcaag gggaattgaa ggtgtcgtta tggaacagaa gcagcgagcc
     1141 gtttactgtc aaaccgtttg agcgtatcgc gcagatggtt atcgtgccaa tcgtgcaggc
     1201 gcgtttcaaa cgtgtcgagg aatttgtcgg aagcagccgg ggtgagggcg gcttcggcag
     1261 tacgggtttg cattgaatat aaaatgccgt ctgatgggtg ccgtcaggtt cagaccgcat
     1321 atcttccgat ataccgataa ccagagaaaa tcatgaatac cttactcaag cagctcaaac
     1381 cctatccctt tgcccgactg cacgaggcga tgcagggcat ttccgcgcct gaaggcatgg
     1441 aagccgtccc actgcatatc ggcgaaccga aacatccgac accgaaagtc attacggatg
     1501 cgctgaccgc ctcattgcac gagttggaaa aatatccgct gacggccggt ttgcctgaac
     1561 tgcgtcaggc gtgtgcgaac tggttaaaac gccgttatga tgggttgacg ctgaattccg
     1621 acaccgaaat tctgccggtt ttaggcagta gggaggcgtt gttttctttt gtccaaaccg
     1681 tgttgaaccc tgtttcagac ggcctcaaac ccgcaattgt cagcccgaat cccttttatc
     1741 agatttacga aggtgcgaca cttttgggcg gcggtgaaat ccattttgcc aattgccccg
     1801 cgccgtcttt caaccccgat tggcgtagta tttccgaaga ggtttggaaa cgcaccaaac
     1861 tggtgttcgt ctgctcgccc aacaacccca gcggcagcgt gctggatttg gatggatgga
     1921 aagaagtttt tgatttacag gataaatatg gtttcattat tgcctcggat gaatgctatt
     1981 ccgaaatcta tttcgacggc aacaaacctt taggctgcct gcaggccgct gcacagttgg
     2041 ggcgaagcag gcaaaaactg cttatgttca ccagtttgtc caagcgttcc aacgttccgg
     2101 gcctgcgttc cggttttgtc gccggcgatg ccgaactgct taaaaacttt ctgctttaca
     2161 gaacctatca cggcagtgca atgagcattc ccgtgcagcg cgcaagcatt gccgcttggg
     2221 atgacgaaca gcacgttatc gacaaccgcc gcctgtatca ggaaaaattt gagcgcgtta
     2281 ttcccatttt gcaacaggta tttgacgtta aattaccgga tgcctcgttt tacatctggt
     2341 tgaaagtccc cgatggcgac gatttggcat ttgcacgcaa tttatggcaa aaagccgcta
     2401 tccaagtatt gcccggacgt tttttggcac gggataccga acagggcaat cccggggaag
     2461 gttatgtccg tatcgctttg gttgccgatg tcgcaacttg tgtcaaagct gcggaagaca
     2521 tagtttcact gtatcggtaa ggaatcggaa atgccgtctg aacttttgtt cagacggcat
     2581 ttttcaatat tctacggttg aatttgctgt aacggtattt atagtggatt aacaaaaatc
     2641 aggacaaggc gacgaagccg aagacagtac agatagtacg gcaaggcgag gcaacgctgt
     2701 actggtttaa atttaatcca ctatacttca cttttaatcg gcttgcccgc aaacacgttt
     2761 aaacttaaaa tccccgtgtt tgacacaata ccgagcagat tatgtttttt gtcctttccc
     2821 ctgcgaagaa ccttaatgaa aaagaccctg cccctgtcag cgagtttacc caacccgacc
     2881 tgctggcaga gtccgacatt ctaatgcagc agttgcgcga gcttgcgccg caacagattg
     2941 ccgaactgat gcacgtttcc gacaaaattg ccctcttaaa cgcgcagcgc aatgcagaat
     3001 ggaacacgcc gtttacgccg gaaaacgcca aacaggcggt ctttatgttc aacggcgatg
     3061 tttacgaagg tatggatgca aacacattgg atattggaca gatacgctat ctgcaaaacc
     3121 atgtccgcct gctgtccggt ctgtacggtc ttctgcgccc gttagacctg atacagcctt
     3181 accgcctgga aatgggaacg cccttcgcca atttgcgcgg caagaatttg tatgagtttt
     3241 ggggcgacat cattaccaac cttttaaatg acacgcttgc ccaagcaggc agcaatacgc
     3301 ttgtcaacct tgcctcacag gaatatttca agtccgtcaa cacgaaaaca cttcgggcgc
     3361 agctgattac cccgatattt aaagacgaaa aaaacggtaa atataaaatc atcagtttct
     3421 atgccaagcg tgcgcgcgga ttaatggtgc gctatgcggc agaacatcat attaccgacc
     3481 ctgaaatgct gaaaaatttt aattacgaag gctacgcatt caatgacgcg gcttcaaatg
     3541 aaagcgaatg ggtttttatg cgttcggaac aaataaagtg aaaacaataa attaagtatt
     3601 ttccgaaaaa agtgcttggc aaaatgtata aacttcatta ttattcttct tcttcaagaa
     3661 gacggaagcg tggcagagcg gtttaatgca acggtcttga aaaccgtcga gggttgatag
     3721 ccctccgtga gttcgaatct caccgcttcc gccaatcctt ggggtcaaaa accaaataaa
     3781 aatgaagtag cataaagcat cggcattttt atttggtttt acttcattat cggaaacgtg
     3841 gcagagaggc tgaatgcagc ggactcgaaa tccgctgagg gtgcaaatcc tccgtgggtt
     3901 cgaatcccac cgtttccgcc acaaaacaaa accgccctga ttcggggcgg ttcttttttg
     3961 ttcaa
//
LOCUS       DAWHXS010000139         3927 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWHXS010000139 DAWHXS010000000
VERSION     DAWHXS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.139"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            212..1729
                     /locus_tag="WIE22_001890"
     CDS             212..1729
                     /locus_tag="WIE22_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ4127910.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1788..3569)
                     /locus_tag="WIE22_001891"
     CDS             complement(1788..3569)
                     /locus_tag="WIE22_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ4127911.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENGRCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIARLPDGAFRIKALLASDG
                     ISLSRAVLNRLTDKQRVIISPTILPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          822 a          879 c         1284 g          942 t
ORIGIN      
        1 cgtactattt gtactgtctg cggcttcgtc gtcttgtcct gatttttgtt aatccactat
       61 aaatgtgaaa tccgcccttt gaaaatcggg cggcggtttt ttgcctgccc gctttcagac
      121 ggcaaagccg gttttcacgg gtttctgcct gtttttcgga tggtttgacg tgcttcggcg
      181 gcgtgtttgc cggaaaggta aatgacaggg tatgttgtat ttcagatacg gctttttggt
      241 tgtttggtgt gcggcaggtg tttctgccgc ctatggggcg gatgcgcccg cgattttgga
      301 tgacaaggca ttgttgcagg tgcagcggtc ggtgtcggat aagtgggcgg aatcagattg
      361 gaaagttgaa aatgatgccc cgcgcgtggt tgacggggat tttttgttgg cacatccgaa
      421 aatgttggaa catagtttgc gcgacgcgct caacggcaat caggcggatt taatcgcttc
      481 gttggcggat ttgtatgcca agctgccgga ttatgacgcg gttttgtacg gcagggcgcg
      541 ggctttgctg gcgaaattgg cgggaaggcc ggcggaggcg gtggcgcggt atcgggaact
      601 gcacggggaa aatgcggcag acgagcggat tttgctggat ttggcggcgg cggagtttga
      661 cgatttccgg ctgaagtcgg cagaaaggca ttttgcggag gcggcaaaat tggatttgcc
      721 ggcgccggtt ttggaaaatg tggggcgttt tcggaaaaaa acggaggggc tgacgggctg
      781 gcgtttttcg ggcggcatca gcccggcggt caatagaaat gccaataatg ccgcgccgca
      841 gtattgccgg caaaacggag gccggcagat atgcagtgtc agccgggcgg agcgggcggc
      901 agggttgaat tatgaaatcg aggcggaaaa actgacggcg ttggcagata atcattattt
      961 gttgttccgt tccaatatcg gcggcacgag ctattatttc agtaaaaaat cagcttatga
     1021 tgacgggttc ggcagggcgt atttgggttg gcagtataaa aatgcgcggc agacggcggg
     1081 gattttgccg ttttatcagg tgcagttgtc gggcagcgac ggctttgatg cgaaaacaaa
     1141 acgggtaaac aaccgccgcc tgccgccgta tatgctggcg cacggagtcg gcgtgcagct
     1201 gtcccatact taccgcccaa acccgggatg gcaattttcg gtcgcgctgg aacattaccg
     1261 ccaacgctac cgcgaacagg atagggcgga atacaataac ggcaggcagg acgggtttta
     1321 tgtttcgtcg gcaaaacgtt tgggcgaatc ggcaactgtg ttcggcggct ggcagtttgt
     1381 gcggtttgtg ccgaaacgcg aaacggtggg cggcgcggtc aataatgccg cctaccggcg
     1441 caacggtgtt tatgccggct gggcgcagga gtggcggcag ttgggcggtt tgaacagtcg
     1501 ggtttccgcg tcttatgccc gccgcaacta taagggcatt gcggctttct cgacagaggc
     1561 gcaacgcaac cgcgaatgga atgtctcgct ggctttgagc cacgacaagt tgtcgtacaa
     1621 aggtatcgtg ccggcgttga attatcgttt cggcaggacg gaaagtaatg tgccgtatgc
     1681 gaaacgccgc aacagcgagg tgtttgtgtc ggcggattgg cggttttgaa tggtggaata
     1741 ataccgtccg aactttggaa acaggttcgg acggcatttt tgcgcgttca ggcaagggcg
     1801 gcggcaaata cgccgcgcaa ggcgttggag aggcggattt cttcggcttc ttgcagtgtt
     1861 ttttgtgtga tgtgtgtttc gattacttga tttgtttgca aatatttttg cggttcgtcc
     1921 aacacggctt ggcgcattat gccgtttaaa atgtctaaat ctaaagaggg cgtgagccat
     1981 tgtccgcgat gtttgacgaa cacgttgctt ctgccgcctt cgagcaggat gccgtctgaa
     2041 ttgaaaaaca ggctgtcgaa cgcgccttgt gtttcggcgg tttgccacgc ttggtcgaag
     2101 acggtgcggc aggtggtttt gaagcggcgc aggtagtttt gtgcgggcag gatggtcggc
     2161 gaaatgatga cgcgttgttt gtcggtcaga cggtttaaaa cggcgcggga caggctgatg
     2221 ccgtctgaag cgagcagggc tttgatgcgg aacgcgccgt cgggcaagcg tgcgatgtat
     2281 tgtttgattt gattttcgca accgtcgggc aggggcaggt tgagggcttg ggcggaggtt
     2341 ttcagacggc ataggtggcg gtcgagcagg gcgcagcgtc cgttttccac gcgcagggtt
     2401 tcaaaaatgc cgaagtcggg gcgcaattcg ttgaggaaac gggctttcca gccgcattcc
     2461 cgatattcgg cggcagggtc gctgtcgatg acgatgccgg aaccgacacc gtacacgcct
     2521 tgataaatgc cgtctgaaag cggcgtgagc gacaaggtgc ggataacgac gttgaacgtg
     2581 ccttcaaacc ccaagccgcc ggaacacggg ttcaaatagc cgatgctgcc cgtataaagt
     2641 ccgcgcgctt cggcttcgag cgattcgata atctgcatac tcattttttt gggcgcgccg
     2701 gtgatgctgc cgcaggggaa ggcggcgcgg aggatgtcgg cgaacgaggt gtgcggcaag
     2761 gcttgggctt ggatggtgct ggtcatctgc caaacgctgc cgaaacgcga tactttaaac
     2821 ggttcgggta cgcatactgt gccggtttgg gcgattttgc cgagatcgtt acgcagcaaa
     2881 tcgacaatca tcacgttttc ggcgcggttt ttcgggtctg cttgcaactc ggcggcgcgg
     2941 cgttcgtctt gtccgtcgcc caaaatcggc gcggtgcctt tcatcggttc ggtgctgatg
     3001 gtgccgtccg aaccgatttt gaggaagagt tcgggcgaga aacacagcgt ccacgcggat
     3061 tgcccttgtg catcgggcag gtgggacaaa acggcatagg ggacgggctg gcgcaggcgt
     3121 tggtaaagtt tgacgggatt gccgtaggct tgcaggtgca ggcgggtagt gtagttgatt
     3181 tgataggtgt cgccgcgtcg gatggcttcg tggatttggc ggatatggtc gaggtaatcg
     3241 gcttcggata cggaggattg cggcgtggaa atgccggcgg ggaggccgtc tgagtgtcgg
     3301 gcaagccagc ttgcggcatc ggtgtcggcg cagtcggcaa accagtgcag ggcaaggttg
     3361 ccgccgcgtt cggactcgac ccccgtcagc ggcaaaccga atccgtagtc tgcaaacaac
     3421 acggcatgca gccctttttg ccagcccgat tgcagcgcgc cgtccaaagc atcgagttct
     3481 tcgggacgga aaaaacggct ttccacatga ttttgatagc gttttgcgcg gccgcttacg
     3541 gcatcgtcaa acagggcgaa ataaggcatg gcaattcggg gcaaatgttt tgattatacg
     3601 ctctttttac acatattttc agacgattcg gcaaatatcg gcaaaatgta attttatgta
     3661 gagaaagtgg ggggcgaagg tgtaaaattg tgaaaacaaa ttccgatttc caacctgaaa
     3721 tacaataagg agacctttat gacagaccac cagctgcaac cgtttgaaaa cgtagaatta
     3781 ggcgaaaagc aagatcagct ccaagtatag tggattaaca aaaaccagta cagcgttgcc
     3841 tcgccttggc tcaaagagaa cgattctcta aggtgctgaa gcaccgagtg aatcggtttc
     3901 gtactatctg tactgtctgc gtcttcg
//
LOCUS       DAWHXS010000140         3897 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWHXS010000140 DAWHXS010000000
VERSION     DAWHXS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.140"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(99..1460)
                     /gene="accC"
                     /locus_tag="WIE22_001892"
     CDS             complement(99..1460)
                     /gene="accC"
                     /locus_tag="WIE22_001892"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ4127912.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(1574..2026)
                     /locus_tag="WIE22_001893"
     CDS             complement(1574..2026)
                     /locus_tag="WIE22_001893"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ4127913.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQ
                     VKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(2008..2265)
                     /locus_tag="WIE22_001894"
     CDS             complement(2008..2265)
                     /locus_tag="WIE22_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ4127914.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2382..3422
                     /gene="queA"
                     /locus_tag="WIE22_001895"
     CDS             2382..3422
                     /gene="queA"
                     /locus_tag="WIE22_001895"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ4127915.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPMQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDSHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAAHETGHLKDGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT          838 a          942 c         1175 g          942 t
ORIGIN      
        1 ttaatccact atatcgaaat cggtttcaga cggcgcgggg caagggaaat gccgtctgaa
       61 cgcggacggg cggcttcaga cggcatatcg gcggcggttt acttgtcctg tttggctttg
      121 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacccgca
      181 tcggcgaaca ggtcgcggtg aagcggcgta ttggttttga tgccggttac cgccagctcg
      241 gcgagtgcga cgcgcatttt cgccattgcc tgttcacgcg ttttgccgtg tacgcagatt
      301 ttgccgatca ggctgtcgta gtacggtggg atgcggtagc cttggtaaat gtggctgtcc
      361 acgcggatac cgaagccgcc gggcaggtgg cagctttcaa tcgggcccgg gctggggatg
      421 aagttgtacg ggtcttcggc attgatgcgg cactcaaacg cgtggccttc gacttgaata
      481 tccttttgtt tgtattgcaa aggcaggccg gcggcgatgc ggagttgctc ctgcacgatg
      541 tccacgccgg taatcagctc ggtaaccgga tgctcaacct gaacgcgcgt attcatctcg
      601 ataaagaaaa attcgccatc ttcgtacagg aattcaaacg tacctgcgcc acgatagccg
      661 atgcgtttgc aggcgtcggc acaggcgttg ccgattttgg cgcgttcttt ttcagtgatg
      721 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
      781 tcggcaaggt agatggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
      841 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
      901 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
      961 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgacttt atcggcgatt
     1021 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     1081 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     1141 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     1201 tcggcaagga aaccgtagcc cggatggaca gcgtccgcgc agcttacttc ggcggcggcg
     1261 ataatggcgg ggacgttaag gtagctttgc gcggaagcgg cagggccgat gcacacggat
     1321 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     1381 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     1441 attaaaactt ttttcagcat gatgactgtt cctgtggttc cggcaacccg tccgtaaaaa
     1501 aaaaggtgcg ggaacgccga agggggaaag catttcagac ggcatcggaa gtttatgccg
     1561 tctgaaaaca ggattatccg atgatgaaga gcggttcgcc gaattcgacg ggcgtaccgt
     1621 tttcgaccag aatttctttg accgtgccgg atttttcggc ttcgatttcg ttcatcagct
     1681 tcatcgcttc gatgatgcac agcgtgtcgc cggctttaac ctgttggccg acttcgacaa
     1741 aaggcgcggc attcgggccg ggtgcgcggt agaacgtgcc gaccataggc gatttttgcg
     1801 cgtcggacaa atcgcgggcg gcaggtgcgg cgggcgcgga tgccgcaacg ggtgcggcgg
     1861 caggcgttac ggccggcgcg gcggcaggta cgggcgcggc gtaaacgggt gcggcggcga
     1921 tggttcgggt gatgcggact ttttcctcgc cttcggttac ttcgatttcg gcgatacccg
     1981 attcttcaac caaatcaatc agttttttta atttgcgcaa atccatttct gttcctttaa
     2041 aggctgccgg tgttccggcg ggttgttgcg ttggtttttc agaaaacagc cggcttgcgg
     2101 caacggcgca aaccgggcgg aatggtgttc ggatacggtt ttcctgtggt ggaaaacaac
     2161 cgtgagaatg tgtttatttt cccgaagttg tcggtaaatg tccagtaaaa tatcaaaaaa
     2221 cggcggtttt tgggggaaat gtgcaggaag tttgattttg cgcacaagat gttccaggtt
     2281 aagagtgttt attctaatct gttggttttt cggacaaaga tgccgtctga aaaagggcta
     2341 aagtgcgtat aatggcggct tgcccgaacg agagtgtaaa aatggatatt tcagattttg
     2401 attttaccct gcccgaaaag ctgattgccc agcatccgcc cgaagtgcgc ggcagcagcc
     2461 gccttttggt tgcgctgccg gatatgccca tgcaagaccg ggtattcggc gatttgcccg
     2521 attatgtcga ggcaggcgac gttttggtgt tcaacaacac caaggtaatg aaggcgcggc
     2581 tgtttgggca gaaagacagc ggcggcagga tcgaagccct gatcgagcgt gttttagaca
     2641 gtcataccgc actggcgcac atccgttcgt ccaaatcgcc caagcccggt atggggctgg
     2701 tgtttgaagg cggcatccgt gccgtgatgg tcgggcgtga gggcgaactg ttctgcctgc
     2761 gttttgaagg cggtcaaacc gtttacgaac ttttggaaca gaacggacat ctgcccctgc
     2821 cgccttatat cgaacgtgcc gccgatgcgg atgacgacag ccgttatcaa accgtttatg
     2881 ccaaatatca gggtgcggtc gccgcgccga cggcgggtct gcattttacg gaagaacttt
     2941 tgcgccgcct gaaagacaaa ggcgcagtaa ccgcagaagt aaccctgcac gtcggtgcgg
     3001 ggacattcca acccgtgcgt gtggaaaaaa tcgaagaaca caaaatgcac agcgaatggt
     3061 ttgaagtgcc gtctgaaacc gccgccgccg ttgaggcggc aaaagcccgg gggaacaaag
     3121 tctgggcggt cggtacgact tccatgcgcg ccctcgagtc tgccgcgcac gagacgggac
     3181 acttgaagga cgggcagggc gacaccgata ttttcatcac gccgggctac cgttttaatg
     3241 ttgccgacag gctggttacc aatttccatc tgccgaaatc gacgctgctg atgcttgtca
     3301 gcgcgttttc gggtatgggt catatccgcg ccgcgtatcg ttatgcggtc gaacgcgaat
     3361 accgtttctt cagctacggc gatgcgatgg ttttggggcg gaacgaaggg gtggtacgtt
     3421 agaacagcct caagtctatt gggggaagac aagtgccgtc tcaacggacg ttcggacggc
     3481 attcttttgt tgatttgaat gaaaggaact gtatgacata tagtgtggat taaatttaaa
     3541 tcaggacaag gcaacgaagc cgtagacagt acaaatagta tggcaaggcg aggcaacgct
     3601 gtactggttt aaatttaatc cactatagat tcaggcgcga gaggcgagaa actcagtcat
     3661 tgttgatgac gcgcaatacg ttaaaattca ttccgttttg gtcgtataaa catattacat
     3721 acgccatttt tttgtattca aggctgcctg aaaacagtgg gcaagaatat ctaacccaac
     3781 gaaaagctga aaaagcaaca aaaaataatc tgcaccttag tcggttggtt agtccaacct
     3841 taggggtgca gaccattcaa tctttcaggc tgcctttgtt tgtttataag ataaatt
//
LOCUS       DAWHXS010000141         3859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWHXS010000141 DAWHXS010000000
VERSION     DAWHXS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.141"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WIE22_001896"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WIE22_001896"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ4127916.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGIAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDDGGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WIE22_001897"
     CDS             1439..1924
                     /locus_tag="WIE22_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ4127917.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIE22_001898"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIE22_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ4127918.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNTKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          777 a          948 c         1081 g         1053 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcatcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaagttttag atgacggcgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgccgag
     2041 tcggcgtttc ttatcggctt cgtattcctg atagttaccg tcgaagaaca cccatttgga
     2101 gtcgccttca cacgccaaga tatgcgtggc gatgcggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctaccgg caaattccag caatgcatct tccagcgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccgccgc tcaacaaggt
     2281 tttcgccaag tgcagacggc cgcgttcgcc gccagacaat tgacctgcga ttttgctttg
     2341 gtcgctgcct ttaaagttga aacgacccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcggc cttcggcaat gttgtcgaat acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaggctcat tttcacggtt tgcccgattt tcacttcgcc
     2521 ggaatcaggc tgctctttgc ccgaaatcat tttgaacagc gtcgatttac ccgcgccgtt
     2581 cgggccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttatcg ccgaacgatt tggaaacatt tacaaattca atcacttcgt tacccaaacg
     2701 ctcggcaacg ggaataaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcctca aaacgcgcca aacgcgcttt ggacttggct tggcggcctt tggtattttg
     2821 gcgcacccat tccaattcct gcttcatcgc tttcacgcgc gcagcttcgg atttcgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accgtgcccg cggtcaagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgagg aattgctcca gccattccac
     3061 cgattccgca tccaagtggt tggttggctc gtccagcagc agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaagcga cgcggcgttt ttcaccgccg gacagattgc cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacccgccg caataatcgc ttccaaacgc ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWHXS010000142         3857 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWHXS010000142 DAWHXS010000000
VERSION     DAWHXS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.142"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(88..906)
                     /locus_tag="WIE22_001899"
     CDS             complement(88..906)
                     /locus_tag="WIE22_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ4127919.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(986..2236)
                     /locus_tag="WIE22_001900"
     CDS             complement(986..2236)
                     /locus_tag="WIE22_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ4127920.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     PNKTRRGSPEADLAQNTTPQPGRGRNGRRPIIMIDPGHGGEDPGAVSSGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WIE22_001901"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WIE22_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ4127921.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYLLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2896..3708
                     /locus_tag="WIE22_001902"
     CDS             2896..3708
                     /locus_tag="WIE22_001902"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ4127922.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          863 a          935 c         1146 g          913 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc
     1021 gttggacacc cgaggcgatg gattgggcgc actgtcggcg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcaggattg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gtgcgcgcaa aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaga tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagt gtggcggttt
     1261 gggtcatgtc gagcagggcg gtatcgacat tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attgttttgg gtttgttcca agaatttggc ggcggagctg gtcgcgcctt
     1381 tggtgttcag catataaacc cctgtgccgc gcgcggaggg gctggtgaaa gcgtcggcgt
     1441 ggatggagac gaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaaag
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaatccg cccgagctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgatgggc ctgcgcccgt
     1681 ttctgccgcg cccgggttgg ggcgtggtgt tttgggcgag gtcggcttcc ggagagccgc
     1741 gccgggtttt gttcgggctg ccgttaagca gcgccatcat cggatcgtcg gcatccatcc
     1801 cttgcggata gagatcgacg acgaggcggt ttttaaagcc gccgacgggg gggagggaaa
     1861 aaacttgcgc gtaggaaggc tgtttcaaat cgatgacgag gcggacggtg tcgggcgtgt
     1921 tctgacccgc gcgtatgctg cggataaagg ggtcgtccgc cataaccttc tgagacagtc
     1981 cgtgcaatac ggtattgatg tttgcgtttt gtatgtcgac gaccagcctg cccgggttgt
     2041 cgagcgtgaa gtgttggtat ttgagcgcgg cggtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgacgg ccatatccgt acggcggtaa actgcggggc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgtcgccgt cgtgtgtcaa tgttgcggtg atgtcggcag gcggggtaaa
     2341 ttccccacct tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 caatccggca tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcaa
     2461 ggtgaagcgt tccagcagat aagattcgac gatggcgtaa gtcgggcttt tgactgcgcc
     2521 ctgatgaccc aatccgcgca ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc
     2581 cccctcgaga taaatgacca gcggcgcgtt taaacgggaa gaccacgccg cacccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggagatagag ggtaaatcag acatggaaac
     2701 ggtttgttgt aaggtctagg gtattatggg cagttttgca ggttttgcaa actttgcacc
     2761 cgaggggcgg atgcttcttg tccgagcatt ataacagcca aatccgcgtt ctgctttcag
     2821 acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata cgcggattgt atgtttagga
     2881 cagattggaa aaaggatgga aaatatcggc aggcagcgac ccatcggcgt ttttgactcg
     2941 ggaatcggcg gtttgaccaa tgtgcgcgcg ctgatggagc ggctgccgat ggagaacatc
     3001 atttatttcg gcgacacggc gcgcgtgcct tacgggacga aatctaaggc gaccatcgaa
     3061 aatttctcga tgcagattgt cgattttctg ctggaacacg atgtcaaggc gatggttatc
     3121 gcgtgcaaca cgattgcggc ggtggcgggg cagaaaatcc gtcaaaagac gggcaatatg
     3181 cctgtgttgg acgtgatttc cgccggcgcg aaagccgcgc tggcaacgac gcgcaacaat
     3241 aaaatcggca ttatcgccac caatacgaca gtcaacagca acgcctatgc gcgcgccatc
     3301 cataggaaca accccgacac gcttgtccgc acgcaggccg cgccgctgct cgtccctttg
     3361 gtggaagagg gctggttgga acacgaagtt acccgcctga ccgtatgcga atacctcaaa
     3421 ccattgcttg cagacggcat cgatacgctg gtgttgggct gcacgcactt tcccttgctc
     3481 aagcccttaa tcggcaggga ggcgggcaat gtcgcgttgg ttgattctgc aattacaacg
     3541 gccgaagaaa ccgcacgcgt ccttgctcag gaaggattgc tcaataccga caacaacaat
     3601 cccgactacc gtttttacgt cagcgatatt cctttgaaat tcagaaccat cggcgagcgt
     3661 tttctgggca ggacgatgga gcagattgaa atggtgtctt tgggttaaaa cgatgacgga
     3721 aagctgcccg agattacaga aacctaaaat cccgtcattc ccgcgcaggc gggaatctag
     3781 acattcaatg ctaaggcaat ttatcgggaa tgactgaaac tcaaaaaact ggattcccac
     3841 tttcgtggga atgacgc
//
LOCUS       DAWHXS010000143         3820 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWHXS010000143 DAWHXS010000000
VERSION     DAWHXS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.143"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            365..709
                     /locus_tag="WIE22_001903"
     CDS             365..709
                     /locus_tag="WIE22_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ4127923.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGTSAPDFNAGGT
                     GIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPRICIP
                     ETLQTQMTHTRI"
     gene            706..3567
                     /locus_tag="WIE22_001904"
     CDS             706..3567
                     /locus_tag="WIE22_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ4127924.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGDGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFSATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1036 a         1038 c         1012 g          734 t
ORIGIN      
        1 cgaagccgca gacagtacgg aaccgattca cttgttaaag aatcgttctc tttgagctaa
       61 ggcgaggcaa cgccgtactg gtttttgttc atccactata actagggaaa ttcaaattaa
      121 gttagaatta tccctataag aaaaagccgt ctaagccggt ataaacaaaa taaactcatt
      181 gagctatttg tcgaaagttc aaatttccat tttaaaacaa ttagtaaaat cgagtttatc
      241 ctagttgtcc aagacaaccc ctataataat ataattcaaa atataaaaat gggttacatc
      301 taaacattac ggaattttta ttccctcgcc tgaattctat tgtcagattc aaggagacct
      361 catcatgcga acgaccccaa ccttccctac aaaaactttc aaaccggctg ccatggcgtt
      421 agctgttgca acaacacttt ctgcctgctt aggcggcggc ggcacttctg cgcccgactt
      481 caatgcaggc ggcaccggta tcggcagcaa cagcagggca acaacagcga aatcagcagc
      541 agtatcttac gccggtatca agaacgaaat gtgcaaagac agaagcatgc tctgtgccgg
      601 tcgggatgac gttgcggtta cagacaggga tgccaaaatc aatgcccccc ccccccgaat
      661 ctgcataccg gagactttac aaacccaaat gacgcataca agaatttgat caacctcaaa
      721 cctgcaattg aagcaggcta tacaggacgc ggggtagagg taggtatcgt cgacacaggc
      781 gaatccgtcg gcagcatatc ctttcccgaa ctgtatggca gaaaagaaca cggctataac
      841 gaaaattaca aaaactatac ggcgtatatg cggaaggaag cgcctgaaga cggagacggt
      901 aaagacattg aagcttcttt cgacgatgag gccgttatag agactgaagc aaagccgacg
      961 gatatccgcc acgtaaaaga aatcggacac atcgatgtgg tctcccatat tattggcggg
     1021 cgttccgtgg acggcagacc tgcaggcggt attgcgcccg atgcgacgct acacataatg
     1081 aatacgcatg atggaaccaa gaacgaaata atgtctgcag ccatccgcaa tgcatgggtc
     1141 aagctgggcg aacgtggcgt gcgcatcgtc aataacagtt ttggaacaac atcgagggca
     1201 ggcactgccg accatttcca aatagccaat tcggaggagc agtaccgcca agcgttgctc
     1261 gcctattccg gcggtgataa aacagacgag ggtatccgcc tgatgcaaca gagcgattac
     1321 ggcaacttgt cctaccacat ccgtaataaa aacatgcttt tcattttttc ggcaagcaat
     1381 gacgcacaag ctcagcccaa cacactgacc ctattgccat tttatgaaaa agatgctcaa
     1441 aaaggcatta tcacagtcgc aggcgtagac cgcagtggag aaaagttcaa tggctccaac
     1501 cattgcggaa ttactgccat gtggtgccta tcggcaccct atgaagcaag cgtccgtttc
     1561 acccgtacaa acccgattca aattgccgga acatcctttt ccgcacccat cgtaaccggc
     1621 acggcggctc tgctgctgca gaaatacccg tggatgagca acgacaacct gcgtaccacg
     1681 ctgctgacaa cggctcagga catcggtgca gtcggcgtgg acagcaagtt cggctgggga
     1741 ctgctggatg cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg cgactttacc
     1801 gccgatacga aaggtacatc cgatattgcc tactccttcc gtaacgacat ttcaggcacg
     1861 ggcggcctga tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa cacctatacg
     1921 ggcaaaacca ttatcgaagg cggttcgctg gtgttgtacg gcaacaacaa atcggatatg
     1981 cgcgtcgaaa ccaaaggtgc gctgatttat aacggggcgg catccggcgg tagcctgaac
     2041 agcgacggca ttgtctatct ggcagatacc gaccgatccg gcgcaaacga aaccgtgcac
     2101 atcaaaggcg atctgcagct gggcggcgaa ggtacgctgt acacacgttt gggcaaactg
     2161 ctgaaagtgg acggtacggc gatgaccggc ggcaagctgt acatgtcggc acgcggcaaa
     2221 ggggcaggct atctcaaccg taccggacaa cgtgttccct tcctgagtgc cgccaaaatc
     2281 gggcgggatt attctttctt cacaaacatc gaaaccgacg gtggtctgct ggcttccctc
     2341 gacagcgtcg aaaaaacagc gggcagtgaa ggcgacacgc tgtcctatta tgtccgtcgc
     2401 ggcaatgcgg cacggactgc ttcggcagcg gcacattccg cgcccgccgg tctgaaacac
     2461 gccgtagaac agggcggcag caatctggaa aacctgatgg tcgaactgga tgcctccgaa
     2521 tcatccgcaa cacccgagac ggttgaaact gcggccgccg accgcacaga tatgccgggc
     2581 atccgcccct acggcgcaac tttccgcgca gcggcagccg tacagcatgc gaatgccgcc
     2641 gacggtgtac gcatcttcaa cagtctcgcc gctaccgtct atgccgacag taccgccgcc
     2701 catgccgata tgcagggacg ccggctgaaa gccgtatcgg acgggttgga ccacaacgct
     2761 acgggtctgc gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga acagggcggt
     2821 gttgaaggca aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa aaccggcgaa
     2881 aatacgacag cagccgccac actgggcatg ggacacagca catggagcga aaacagtgca
     2941 aatgcaaaaa ccgacagcat tagtctgttt gcaggcatac ggcacgatgc gggcgatatc
     3001 ggctatctca aaggcctgtt ctcctacgga cgctacaaaa acagcatcag ccgcagcacc
     3061 ggtgcggacg aacatgcgga aggcagcgtc aacggcacgc tgatgcagct gggcgcactg
     3121 ggcggtgtca acgttccgtt ttccgcaacg ggagatttga cggtcgaagg cggtctgcgc
     3181 tacgacctgc tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg ctggagcggc
     3241 aacagcctca ctgaaggcac actggtcgga ctcgcgggtc tgaagctgtc gcaacccttg
     3301 agcgataaag ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa cggacgcgac
     3361 tacacggtaa cgggcggctt taccggcgcg actgcagcaa ccggcaagac gggggcacgc
     3421 aatatgccgc acacccgcct ggttgccggt ctgggcgcgg atgtcgaatt cggcaacggc
     3481 tggaacggct tggcacgtta cagctacgcc ggttccaaac agtacggcaa ccacagcgga
     3541 cgagtcggcg taggctaccg gttctgacgg acaggaagca gacagccgca aagatcacgg
     3601 tctttgcggc tgtttcttat gaaaagaaaa ccctattcca attgcctgct tctattgttt
     3661 caagacttct tccaaagatt cggcattaat cagatgtata gcggattaac aaaaatcagg
     3721 acaaggcggc gaagccgcag acagtacaaa tagtacggaa ccgattcact cggtgcttga
     3781 gcaccttaga gaatcgtttc tttgagctaa gtcgaggcaa
//
LOCUS       DAWHXS010000144         3804 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWHXS010000144 DAWHXS010000000
VERSION     DAWHXS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.144"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            96..275
                     /locus_tag="WIE22_001905"
                     /pseudo
     CDS             96..275
                     /locus_tag="WIE22_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(272..778)
                     /locus_tag="WIE22_001906"
     CDS             complement(272..778)
                     /locus_tag="WIE22_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ4127925.1"
                     /translation="MAHPQEIREKLRRFYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(781..978)
                     /locus_tag="WIE22_001907"
     CDS             complement(781..978)
                     /locus_tag="WIE22_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127926.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(975..1241)
                     /locus_tag="WIE22_001908"
     CDS             complement(975..1241)
                     /locus_tag="WIE22_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127927.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1253..1591)
                     /locus_tag="WIE22_001909"
     CDS             complement(1253..1591)
                     /locus_tag="WIE22_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127928.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(1588..1806)
                     /locus_tag="WIE22_001910"
     CDS             complement(1588..1806)
                     /locus_tag="WIE22_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127929.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1819..1989
                     /locus_tag="WIE22_001911"
     CDS             1819..1989
                     /locus_tag="WIE22_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ4127930.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(2034..2339)
                     /locus_tag="WIE22_001912"
     CDS             complement(2034..2339)
                     /locus_tag="WIE22_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127931.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(2236..2655)
                     /locus_tag="WIE22_001913"
     CDS             complement(2236..2655)
                     /locus_tag="WIE22_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127932.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQVVIE
                     KMKRDKAQAMLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            complement(2627..2863)
                     /locus_tag="WIE22_001914"
     CDS             complement(2627..2863)
                     /locus_tag="WIE22_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127933.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETASE"
     gene            complement(2866..3030)
                     /locus_tag="WIE22_001915"
     CDS             complement(2866..3030)
                     /locus_tag="WIE22_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127934.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(3033..3239)
                     /locus_tag="WIE22_001916"
     CDS             complement(3033..3239)
                     /locus_tag="WIE22_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127935.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(3236..3778)
                     /locus_tag="WIE22_001917"
     CDS             complement(3236..3778)
                     /locus_tag="WIE22_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ4127936.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGHHSRLAYAQAGGIVEPFFISNDTDLALFKTTK
                     WGICRAIADAIAMKLGAARV"
BASE COUNT          878 a         1081 c          853 g          992 t
ORIGIN      
        1 tttgggtaga attaggaggt atttggggcg aaaacagccg aaaacctgtg tttgggtttc
       61 ggctgtcggg agggaaagga attttgcaaa ggtctcgggc tttaattgcg gcaaaaatag
      121 accaagcgga gatttctgat tcagaaaaaa gctggttgaa aaaagaatta gggaaaatca
      181 aggacactgc attaagtacg cttacggaaa atgcaattaa tgctatccct gccgccaccc
      241 tcattactct tttaaaaaaa tttgttggcc tctaaccaaa cttcttctcc acttccgccc
      301 caaacggctc caatacctcc acaaaggcag gcaaatgttt gggatgtttt tcttgcacaa
      361 acgccatcaa gaactcaatc aattccaaag ccgtcgccag ttttgacgtt tccggcatca
      421 cacgcgcatt ggccgatacg gttttcgtaa acgcatcggc caggctggcc aacagcttgg
      481 cgcggtcgga cgggggcaaa tcttcggtac tcgaatcctg cagcatcgtc atcgtgctgt
      541 tgtactgcac cataaaaccg gccaacatcg cacggctcaa gtcctcaata ccgccgccgg
      601 ccaaagtgta ggcggcgcgc atcttatccc agtcgtcgcc tttttcctta tccgcacgtt
      661 tccacgcacg cgcagtggtc tgcgggattt cgcacatcaa ggccgccgtt tccaaagttt
      721 gctcgccgct cacatagaac cgacgtaact tttcacggat ttcttgcggg tgagccataa
      781 ttacagtccc attttcgctt taagcaattc ccagccgacc gtaatcacgc cgccgcccag
      841 tgcgccgaat gtaatggccg tgcgtttcgt gtcttggcgg atttgcgcaa tttccgcctg
      901 catttctttc tgatttttca gcgtttgatc agtcttgttt tcaatacgcg ccaaggcttc
      961 caaaatcggg tcgctcatga tttgtctgct ttcctgtcca gtttttcgtt tactttttcc
     1021 aacttgtttt cgatgcgtcc caacgaagcc gcaatattgt cgcggtctgc cttggcatct
     1081 tgcttggtgt gataagagag cttgaccgcg tgcagctcct ctttcaggtc gtcgatacgc
     1141 ttgtccgcct ctttcagacg gcctgaaatg ccgttgaccc aaaaccaaaa tgccgccgtc
     1201 gcaatcggcc acagggttct gaaaccaaat tcaaagtcca tttaaaaccc ctttaaaccg
     1261 gcacatcgcc gaatacgata cggacggcgt aaccgtcagg gcgattgctg gcaatctcca
     1321 gtccatcacc atcattacaa acacagtaat acgcccccaa agtttgccaa accgcacgtt
     1381 taaaggtgtc atagttcgta attggatatt caaggtgaaa cgtcgtctga aaatccttat
     1441 ccatgcgtac cgcgtactca atgcctgcct tatccaacag atcggaaaca tgaatgacaa
     1501 acggctcttg ctcgcgtgcg cggcttaagc ccaattccaa atcggcatgg cgcacagcca
     1561 actgacgttc aactaattca cggtaggtca ttctttgata cccattaaat attttgtccg
     1621 cttgtacaat ttcttaaccc acgaaatatt tacaaatgta taaacctttg ctacaacttc
     1681 gccgtcatag tgcgcatttt ctcgtgcagc ccgaaatttc gccctggctt cttcagggtt
     1741 gtccgcccaa atgctcaatg accaggactt gccgttaaag cggtaagaaa acgtgtactc
     1801 attcatagga gaaaccttat gtattttgaa atctataaag acgcaaaagg cgaataccgt
     1861 tggcgtttga aagcagccaa ccatgaaatc atcgctcagg gcgaaggcta caccagcaag
     1921 caaaactgtc agcacgcagt cgatttgctg aaaagcacta ccgccgctac ccctgtaaaa
     1981 gaggtataaa atccgctttc accctcagcc cgcgccctac gcgggctttt ttgtcagttt
     2041 ccgactttgc gggagtgatt gcccgcccag tcgcgccaag cctgattttg attttccaac
     2101 tccgccacat agccgccaaa ctcggcggcg tgttccaaca gcgtggccgt cttgccgtct
     2161 ttcggcggat ttgggcgtac cggcgcgacc atcaatgcgg caggcggtgt cggcatgacc
     2221 gccttttcga caaccttaat ttccgtagcc gagggcgcgc ttgtagagct gcaagccgtg
     2281 atggccaaag ccgtcaatac aaccgccgcc tgcattttta cggtcttgag taaggacatt
     2341 ttcgatttcc tttttatttt ccgttttcag acggctgact tccgcctgtt ttttcgccaa
     2401 agccatgccg acggcgtgcg ccttgacttc atattttttc gcttccgcgc gcgcctgttc
     2461 cagctcgcgt gcatagtttt gagccgacaa cagcatggct tgcgccttgt cgcgcttcat
     2521 tttctcaatg accacctgct gcttcgcaaa cgccgactta tagccttgat ggtgcgacac
     2581 cgccaaaccc gtgccgacaa gcgcgataat ggcaattggt tgccagttat tcgctagcag
     2641 tttcacgaga ttcattctcg acctcctgac gtttgacgct gaccaacgaa cgcgccaccg
     2701 catagccgcc gacaatgccc aaatacaccg cccaaatctc cgccgatggg tcgggcaaca
     2761 tcacaaattt aaacgtcccc gccgcgcagg cgacgtttgc ccacagcttc gagtgcgaca
     2821 cattgcctgt cgccgggttt ttaaaaatat ccaaaatacg cattgctatt ccacactttt
     2881 ggtttgcagg tgccgtttca gcatttcctg ataattggcc agttcgccct ccgcaaattc
     2941 aaacgccgcc aagtctgcct gttcgcttgc ctcacggctt ttggccgacc acagcccaat
     3001 catcttttcg taaaacgcaa cctgtcccat gattaacgac gattcttgcg tttgcgcgca
     3061 gcccgtttcg cggctgccac gcctgagttt cttaaatttg ggcgtggata ggtgtgtatc
     3121 ggacgaacat ttggcatctg tataagtgcg cggtttacct gctcattact aggattttct
     3181 ccaatcaatt ttgtaagaaa ataggaagca gcttctttca atttaccaat aatattcata
     3241 ctcttgccgc ccccaatttc atcgcaatcg cgtccgcaat cgcgcggcag atgccccatt
     3301 tggtcgtctt aaacaaggcc aaatcagtgt cgttgctgat gaaaaaaggc tcaacaatgc
     3361 cgccagcctg cgcataagcc aggcgcgaat gatggcccgc attgtcgggt ttaaagccgt
     3421 cttcgccgcg cagtttccag ccggttttct tggcaacagc tttgctcaac acctgacacc
     3481 agcgtttgtt tttaacggta ctcaacgcct caatgcccgt agctgcttta ccggccgccg
     3541 agttggtatg gaactcaatc gccacatccg agccgcgaat cagttttaca gcttcacgca
     3601 gcggcatatt gcctttgcct gtgccgtcgg ttttaacagt caaaccgtag tcatcgcgca
     3661 aaatagcagc cacaatgttg cgcatatcct gcgccaagtc cgcctcacgg tcgctgccgt
     3721 tgaccgcacc cgggtcggtg ttgctgtgtc cagcggttaa ggttacggtt ttgcccattc
     3781 atcatctcca aaaaatattg attg
//
LOCUS       DAWHXS010000145         3668 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWHXS010000145 DAWHXS010000000
VERSION     DAWHXS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.145"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..562
                     /locus_tag="WIE22_001918"
     CDS             <1..562
                     /locus_tag="WIE22_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ4127937.1"
                     /translation="DGTTPADWVAMDLFSQAEHDEIAQAILIGTSQAYLDEVEAAMDR
                     LIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVENPQEWAKKIRH
                     AGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSSLIQVSEQGAQK
                     LGETASVLAHGESLTAHARAAEFRMK"
     gene            608..1705
                     /locus_tag="WIE22_001919"
     CDS             608..1705
                     /locus_tag="WIE22_001919"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ4127938.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            1702..2619
                     /gene="hisB"
                     /locus_tag="WIE22_001920"
     CDS             1702..2619
                     /gene="hisB"
                     /locus_tag="WIE22_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ4127939.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPNARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            2714..3583
                     /locus_tag="WIE22_001921"
     CDS             2714..3583
                     /locus_tag="WIE22_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ4127940.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQVGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT          943 a         1208 c          855 g          662 t
ORIGIN      
        1 cgacggcacg acacctgccg attgggtggc gatggatttg ttcagccagg ccgaacacga
       61 cgaaattgcc caagccatct taatcggcac gtcgcaagcg tatctcgacg aagtagaagc
      121 cgctatggac cgcctgatcg aaactatgcc gcgccgcgac atcatcgaag cctcgctcgg
      181 caacaggggc gcgatgatac tcgccaaaga cttggacgaa gcctgcgaaa tcgccaacta
      241 catttccccc gaacacttgg aactgtcagt cgaaaatccg caggaatggg cgaaaaaaat
      301 ccgccacgcc ggtgcgattt tcatgggacg ctacaccggc gaaagcctcg gcgactactg
      361 cgccggtcca aaccatgtat tgcccaccag ccgaaccgcc cgcttttcct cgcctttggg
      421 gacatatgat ttccaaaaac gctccagcct gattcaggtt tcggaacagg gcgcgcaaaa
      481 attaggcgaa accgccagcg tgctggcaca cggcgaaagc ctgaccgccc acgcccgcgc
      541 ggcagagttc cgtatgaaat aatgccgaaa cggcgtacag gcatattcca accattaagg
      601 aaacacgatg aaatccgtcc gctccttcat ccgcgacgac atacaagcta tgtcggcata
      661 tcagattgcc gacgttccgc ccggctttgc caaactcgat tcgatggaaa gtcccgtcca
      721 cccttttgcc ggacatgaaa cgctgttgca ggaatggcag gcacggcttg ccgccgcgcc
      781 catccatctt taccccaatc cctccggcag cggtttacag gaagcattac gttcggcgtt
      841 cgacattccc gactgcgccg acatcgcgct gggcaacggc tcggacgagc tgatacagtt
      901 catcacgatg ctgaccgcca aaccgggcgc ggcaatgttg gcagccgaac ccagtttcgt
      961 catgtaccgc cacaacgccg cgctgtacgg catggattat gtcggcgttc cactgaacgg
     1021 agatttcacc ctcaacctgc ccgccgtcct cgaagccgtc aggaagcacc gccctgccct
     1081 gacctttatc gcctacccca acaaccccac cggcgtatgt ttcacgcgtg ccgaaatcga
     1141 agccgtcatc gaagcttcag acggcatcgt cgtcgtcgac gaagcctacg gcgcattcaa
     1201 cggcgacagc ttcctgccgc aggcgggcag cattcccaac ctcatcgtca tgcgtaccgt
     1261 cagcaaaatc ggttttgccg gactgcgtat cggctatgcg gcaggctgtc ccgaagtcat
     1321 cggcgaactg caaaaaatcc tgccgcccta caatatgaac caactgagcc tgaccaccgc
     1381 caaactcgcc ctgcaacact acggcatcat ctctgccaac atcgacagcc tgaaaaacga
     1441 acgcgaacgg atgttcgccg aattgggcaa aatatgccgt ctgaacacct ttccaagtca
     1501 ggcaaacttc attaccatac gcgtacccga tgccgatttg ttgtttgaca cgctcaaaca
     1561 aaaccgcatc ttggttaaaa aactgcatgg cgcgcacccg cttttggaac actgcctgcg
     1621 cattaccgta ggcagccccg cacaaaacga tgccgttctc aacatcattc gccaacttta
     1681 ctgccaacca acggatttcc tatgaatttg actaaaacac aacgccaact gcacaacttt
     1741 ctgaccctcg cccaagaagc aggttcgctg tccaagctcg ccaaactctg cggctaccgt
     1801 acccccgtcg cactctacaa actcaaacaa cgccttgaaa agcaggcaga agacccaaat
     1861 gcacgcggca tccgtcccag cctgatggca aaactcgaaa aacacaccgg caaacccaaa
     1921 ggctggctcg acagaaaaca ccgcgaacgc actgtccccg aaaccgccgc agaaagcacc
     1981 ggaactgccg aaacccaaat tgccgaaacc gcatctgctg ccggctgccg cagcgttacc
     2041 gtcaaccgca atacctgcga aacccaaatc accgtctcca tcaacctcga cggcagcggc
     2101 aaaagcaggc tggataccgg cgtacccttc ctcgaacaca tgatcgatca aatcgcccgc
     2161 cacggcatga ttgacatcga catcagctgc aaaggcgacc tgcacatcga cgaccaccac
     2221 accgccgaag acatcggcat cacactcgga caagcaatcc ggcaggcact cggcgacaaa
     2281 aaaggcatcc gccgttacgg acattcctac gtcccgctcg acgaagccct cagccgcgtc
     2341 gtcatcgacc tttccggccg ccccggactc gtgtacaaca tcgaatttac ccgcgcacta
     2401 atcggacgtt tcgatgtcga tttgtttgaa gaatttttcc acggcatcgt caaccacagt
     2461 atgatgaccc tgcacatcga caacctcagc ggcaaaaacg cccaccatca ggcggaaacc
     2521 gtattcaaag ccttcgggcg cgccctgcgt atggcagtcg aacacgaccc gcgcatggca
     2581 ggacagaccc cctcgaccaa aggcacgctg accgcataaa aaaccatacc gtctgaaaca
     2641 cccgcaggct tttcagacgg tatcggaaca gataagatta cactacacta caaacagaaa
     2701 aggagtaaac atcatgtccg caaacgaata cacacaaatc ggctggatag gcttagggca
     2761 aatgggtctg cctatggtaa cgcggctctt ggacggcggc atcgaagtcg gcgtatacaa
     2821 ccgctcgccc gacaaaactg cccccatctc cgccaaaggc gcaaaagttt acggcaacac
     2881 cgccgaactc gtccgcgact atcccgtcat tttcctgatg gtttccgact atgccgccgt
     2941 gtgcgacatc ctgaacggag tccgcgacgg attggccggc aaaatcatcg tcaacatgag
     3001 caccatctcc ccgaccgaaa acctcgccgt caaagcactt gtcgaagccg caggcggaca
     3061 gtttgccgaa gcacccgttt ccggatcggt cgggcccgcc accaacggca cgctgctgat
     3121 tctgttcggc ggcagcgaag ccgttttaaa cccgctgcaa aaaatatttt ccctcgtcgg
     3181 caaaaaaacc ttccatttcg gcgatgtcgg caaaggttcg ggcgcgaaac tcgtcttgaa
     3241 ctcgctcttg ggcattttcg gcgaagcgta cagcgaagcg atgctgatgg cgcggcagtt
     3301 cggcatcgat accgacacca tcgtcgaagc catcggcggc tcggcaatgg actcgcccat
     3361 gttccaaacc aaaaaatccc tgtgggcaaa ccgcgaattc ccacccgcct tcgccctcaa
     3421 acacgcctcc aaagacctca acctcgccgt caaagagctt gaacaggtag gcaacaccct
     3481 gcccgccgtc gaaaccgttg ctgccagcta ccgcaaagca gtcgaagccg gctacggcga
     3541 acaggacgtt tccggcgttt acctgaaatt ggcagaacac tgattgcctt ttccaaacac
     3601 aatgccgtct gaacatattt cagacggcat ttttatcacc ccacgcttaa aatcagtccc
     3661 gattatga
//
LOCUS       DAWHXS010000146         3617 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWHXS010000146 DAWHXS010000000
VERSION     DAWHXS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.146"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            157..591
                     /locus_tag="WIE22_001922"
     CDS             157..591
                     /locus_tag="WIE22_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ4127941.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(604..1468)
                     /locus_tag="WIE22_001923"
                     /pseudo
     CDS             complement(604..1468)
                     /locus_tag="WIE22_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1476..2093)
                     /locus_tag="WIE22_001924"
     CDS             complement(1476..2093)
                     /locus_tag="WIE22_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ4127942.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKSNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2256..2870)
                     /locus_tag="WIE22_001925"
     CDS             complement(2256..2870)
                     /locus_tag="WIE22_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ4127943.1"
                     /translation="MKTSNFSKRTSAMKTFALILAGGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLLGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQIDLQEG"
     gene            3076..>3617
                     /locus_tag="WIE22_001926"
     CDS             3076..>3617
                     /locus_tag="WIE22_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEZ4127944.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLML"
BASE COUNT          826 a         1031 c          838 g          922 t
ORIGIN      
        1 actggttttt gttaatccac tatacgttcc gattccgccg ttatgtctgc ctgccggaca
       61 tacgtacgca ttattaacaa aagttaaccg cgataatacc atctttcaca cgtcaatcta
      121 gtatatttcc taaaatttcc aacaagagga aaagccgtgc cactgcctgc tccctgccgt
      181 tttgccaaac ctgccgcctc ttttttaagt atggctttgc tttcctgcca gctttcccac
      241 gccgccacgg cttatatccc cctgaacgat tttcaaccga actgcgacat acgccggctc
      301 ggactgacac agggtcagca caatgaactg cgtaaaatcc gcgccgcctt caaaatggcg
      361 ggcgacaggg cgcgtttgaa ggttatgcat tccgaacaca gccgccgccg gtctgtcgtc
      421 gaaatcattt cttcggatgt ttttaatcgg aacgaggcgc gcgattatgt cgaaagccgc
      481 tatttgtccg gtatggattt tgcggtggac gaattggaaa tccaacaccg cttcttccat
      541 atcctcacac cgcaacagca gcaaatgtgg ctttcttcct gcctcaaata atccccgaaa
      601 cgctcacggc gcacgttgct ccggcagcct gcccgcccag tcgcaggcaa actgccacgc
      661 ggaacgtccc gaacggctgc cccgcgtctg cgcccactgc aatgccgcca tctgtgcggt
      721 ttcatcatac ggcacgccga aatcttccag ccaattttgc accgccgcca gataatcgtt
      781 ttgatcgaac ggataaaaac tgagccacaa tccgaatcgg tcggacaggg atattttttc
      841 ttccaccgct tctttctgat ggacttcccc cccgcatccc cgtcgtaccg gcattctcgt
      901 caaaatattc gggcatcagg tgccgtctgt tggaagtcgc gtaaaccaaa acgttggcgc
      961 aacgttgaga cagaccgccg tctaacgcgg ttttcaatgc cttataggtt tcatcgccgc
     1021 tttcaaacga caaatcgtcg caaaatacga taaatttttc cggacgttcc ttcaaaagcg
     1081 tcaacaggta aggcaggccg attaaatcgc ttttatcgac ttcgatcagg cgcaatccct
     1141 tattcgcata ttcgtgtagc agggctttga ccagcgagga tttgcctgtt ccgcgcgcgc
     1201 cgctcatcaa tacgttattc gcgggtctgc cgacaatgaa ctgttcggta ttacgcacca
     1261 gcaattcggt ttgcctgccg actcccgcca gccttaccaa gggaaaggtg tgcggatcgg
     1321 gcaagtgttc caaaaaacct tttttgcccg cactctgcca gcggaaggca agcgcgttcc
     1381 aatccgtatg cccgggttcg ggcggaagca cggcatccaa acgccgcaaa acggcatagg
     1441 ctttatcgag aaattcgttc aattccatct ctacctcact ttgcatatct ttgcgccatc
     1501 agccgttcga cggtgtcgac gattgcctgc gtattcggat cgatttcgat gttgatcctg
     1561 tcgccgacct ttctgctgcc gaacagcgtc cgttccaaag tttcgggaat cagatggaca
     1621 ttgaaacggc cgtcttcgac tttgcctatg gtcaggctgc aaccgtccaa gccgacgaac
     1681 cctttggtca ggatataggg tttgagttca tgcgggagcg aaaaccaaac cgtgcggttg
     1741 aacccgtccc gttcgatttc gacaataggc acggttgcca taatgtgtcc gctcatgacg
     1801 tgtccgccga tttcgtctcc gaaacgcgcc gcccgttcga tgttgacgca atcgccttcc
     1861 ttcagcagcc ccaaattgct ttttgccaaa gtttccgcca ttaaatcgaa gctgacgcgg
     1921 tttccttcga tttcggtaat cgtcaggcag cagccgttat tggcaaccga tgcgccgcgt
     1981 tgcagattgt ccgccgcctc ttgcggaagc tcgacgacat aagtctgaaa tgcctccgac
     2041 gggcggtgga ttgccgtcag ttttcccaat ccttgaacaa tgcctgtaaa cataatcctg
     2101 tttccctgtg tcggtaaaaa tggtgcaaat tgtagcatct ccccgcgaaa aatgccgtct
     2161 gaaatgcctt cagacagcat tatgcctccg attcgggcaa aaaccgcccg gtatggcttg
     2221 acctttcctt tccacgccgg tcggcggtct tgcccttatc cctcctgcaa atcgatttgc
     2281 gtgttcaagt cggcaaaatg cccgtcaaac tcgaatctga ccgaccgcgc cctttgctgc
     2341 tgcaaccagc ttcttaatgt tttcataccc gaaaacagat agggaatcgc gctttgcaga
     2401 atctgcgggc ggatatacat aatgttgtag tgcatcgtta tcggcgtttc cacataatac
     2461 gcattgcaca acggtgtgcg tttcgacacg gtttcaaacc tcgccaccaa atcgcccagc
     2521 agatacggca tatcgcacgg cacaaccaaa agccagtcgg cagtcgccaa ctgcaaatcg
     2581 ttggctgcgg tacacaatgc cgaaagcggg ccgaaatgct gccactgccg tgcgtccgga
     2641 aaaatatgcg gacttcttcg agcatattct tccaaattcc ggttggtgct gatggcgata
     2701 tggctgacct gcggcctgac cctgtcgatg acatgatcta tcagtgcctt accccccaaa
     2761 agagcaagcc ctttgtcctc gcctcccata cggctcgcct gaccgccggc cagtatcagg
     2821 gcaaaagttt tcattgcgga tgttctcttg gaaaagttcg aggttttcat gattgcagtc
     2881 tgccgttccc aatgactcaa aatgccgtct gaagcagacg gcaaataaat tcatattatc
     2941 tgaattttat cataacatga tttaacactg aagcggtgcg gattaagttt tccttaacca
     3001 attctttctg agcagttgta tctaattcca aagaatgata ttgtttgcat tatttagaac
     3061 aatttttcgc cgagcatgat actgccagcc cgtttttcag acggcatcag cctttccctg
     3121 cgcctgaaac tcctgaccgg actgtgggtc gggttggcgg cattgtctgt cgttttgaca
     3181 ctgctgctct ctttgcgtct ggaaaacgcg gcctccgtca tcgaagaggc gggcaacttg
     3241 agaatgcagg cataccgtct ggcatacatg gcgggtgaag gctcgccccg tgcgcaaatt
     3301 gacaatcagg ttgccgaatt tgaaaaaagt ttaaaacgca ttgcccaaag cgatgccatc
     3361 catccgctga ttccttcgga cacccctctt gcttatgatt tgatacaatc catgctgatt
     3421 atagattggc aggcacacat cctccccccg ctccagtcct accggcgacc gactcaggtc
     3481 gatctctacc gctttgccgg aaacatcgaa ctgtttttgc aggcattgga aaatgccaac
     3541 gaaaaaaaca catggtggct caggcgtttt caatgggcaa ttatgttgat gacgctggtg
     3601 tcgtctgtac tgatgct
//
LOCUS       DAWHXS010000147         3577 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWHXS010000147 DAWHXS010000000
VERSION     DAWHXS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.147"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(106..1461)
                     /locus_tag="WIE22_001927"
     CDS             complement(106..1461)
                     /locus_tag="WIE22_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ4127945.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1658..2479
                     /gene="nadE"
                     /locus_tag="WIE22_001928"
     CDS             1658..2479
                     /gene="nadE"
                     /locus_tag="WIE22_001928"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ4127946.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2549..3022)
                     /locus_tag="WIE22_001929"
     CDS             complement(2549..3022)
                     /locus_tag="WIE22_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ4127947.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            3324..>3577
                     /gene="trxA"
                     /locus_tag="WIE22_001930"
     CDS             3324..>3577
                     /gene="trxA"
                     /locus_tag="WIE22_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEZ4127948.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFK"
BASE COUNT          849 a          954 c          945 g          829 t
ORIGIN      
        1 cgctttttca ggaatgacga attaaagatt atcttaaggt caagggactg gactcccgct
       61 tttgcgggaa tgacggcgga gggggagcgg tttttccgat tgggtttaaa tgcaatcgaa
      121 caaatcctgc tgcccttgtt ctttgcttac gcgcacgtcg gtttcgccgt cggcgaaggt
      181 aatgtgcagc ttctgcccct gcttcaaaac atcggcgttg cggatgactt gtccgcgtgt
      241 gtttttgacg acggaaaagc cgcgctccag aatgtgctgc ggcgaaacgg cttcgagcaa
      301 tgcggcttgg gcggtcaggc tttggcggcg gtgggtaagc agttggctga aggcggcggg
      361 caatgccgtc tgaaggcgga cgatgttgtt tttgcaaacg gaaacatcag gacggtaatg
      421 ttgcagggct tgggtttggc gttcaaaacg ggcggtgtgg gcacggaggt tttgcttcat
      481 cgagtaagac agcgtttgcg ccagcttgcc gattgaagcg cgctgctcat caagtttttg
      541 gcgcggatga cggatttgcc gcgcaagcca gtcgagtttt tggctggcat cgaaatagcg
      601 ttgttccaaa acggttttca gacggccttg cgcctgagcg agacgatgta gcgattcttg
      661 gcggttgggg ctgaccagct ccgccgcacc ggtcggcgtg ggcgcgcgca cgtcggcgac
      721 aaaatcggca agcgtaaaat cggtttcgtg tcccacgcca ctgacgacgg gaaccgtgca
      781 ggcttcgatt gcgcgcacga ccggttcttc gttaaacgcc cacaagtctt caatgctgcc
      841 gccgccgcga cagacaatca acacgtcgca ttcggcgcgt tgcgaggcgg ttttaatcgc
      901 ttgggcaatt tgtaactcgc tgcctgcgcc ttgaacaggt gtcggataaa cgataacggg
      961 aatttcgggc gcgcggcgtt tcaaggtggt aacgacatcg cgcaatgccg ctgctgccag
     1021 gctggtgacg atgccgatac attgcggacg ggtaggcaac ggctttttgc gttccgccgc
     1081 aaacgcgcct tccgcctgca actgtgcctt caaccgctcg taggcttcgt aaagctgccc
     1141 caaacctttg agccgcacct cattcacggt aatttgaaat tcgccccgcg cctcgtaaat
     1201 actgattttt cctgatactt cgatatggtc gccttctttc aaaggcttcg ccaaacgcgc
     1261 cgccgcacct ttgaacatcg cgcaacgcac ctgcgcgcgg ctgtctttga gcgagaaata
     1321 ataatgtccg ctggcggcac gggtcaggtt ggacacttcg cccgcaatcc acaggccggc
     1381 aaggtggttt tccaaaagac ttttggcaaa tgcgttcaac tcggaaacgg acaacacgtc
     1441 agaatgaaaa aaatcagaca tagaatcaat caaataataa aaaatatgaa tatgttttga
     1501 agcctaaggc ggtattagtc cgcctaaatt gtcaacaata ttataacacg cgccatcttg
     1561 ccgcccgcct tttcccgtat gactttttta agcggggaat gggaaaaata ttcatcaacc
     1621 cgccctcaat ctattcaaat tacaccgccg acaagctatg atgcggatat tttcgacagg
     1681 aggaaaaatg gatacgcagg cagttatcac acatattgtc cgatggttgg acgaatacgc
     1741 cgcccgggca aatgcaaaag ggtttgtcgt gggcgtttcc ggcggcatcg attccgccgt
     1801 cgtctccgca ctcgccgccc gcaccggccg caccacgctg cttctggata tgccgatacg
     1861 ccaacacccc gaccagcttg agcgggcaag gctgcacatc cgcaatctgc aacggcaata
     1921 tgccaatgta agcgcacaaa cggtcgatct gaccgacacc ttccagacct ttgaacaaac
     1981 cgtcggcgtt catcagacgg catttgacaa tcagccgctt tccctcgcca acgccagaag
     2041 ccgcctgcgt atgctgaccc tgtactacta cgggcagata cacggactac tggttacggg
     2101 gacaggtaac aaaattgaag atttcggtgt aggatttttt accaaatacg gcgacggcgg
     2161 cgtggacatc agcccgattg ccgacctgac caaaacccag gtttaccggc ttgccgaagc
     2221 attgggcgtg gacgaggcga ttcaaaaagc cccgccgacc gacggcctgt gggatacgga
     2281 acgcaccgac gaagaacaga tgggcgcaag ctatcccgaa ctggagtggg caatgggcgt
     2341 gtacggcacg cgcaaacccg aagattttga agggcggcag cgcgaagttc tggaaatcta
     2401 tacgcggctg caccgcgcga tgcagcacaa aatcaacccg attcccgtgt gccgcattcc
     2461 gcccgaattg ttgggctgaa acgtggaaat gccgtctgaa gcggaaaacc gtgtttcaga
     2521 cggcattgac atatccggct cctatccctt aagaatcgag tacgcgggca aacaaaatat
     2581 cgttttccaa atgaatgtgg tcgttcaaat cctccaccat ttctttggcc aaagcgtaaa
     2641 gccgcgtcca gcttccgcag gctccttcgg gcgcttggaa attatcggtc agctctttga
     2701 gccttgcgat ggcgcggtcg tgttcttcat gttcgtgcat catcacgctg atgggcatcg
     2761 ccgcaccccg accgacaccc tgattaatca tcggaaacag catcctttcc tctttcatca
     2821 tatgcatcag cagttcgttc tgcatatagg caagcagctc ggcaatttcc gccggaaagg
     2881 tatcggcatg aacttgggcc actttttgcg ccagcggcac caattcttca aattgtgcac
     2941 ggtgtacatt gtggtagcgt tgcaggatat gatcgacggt tgcaccaaag ggagcagttt
     3001 cccaaacgga aaaatcagtc atggcagggt tccttttatt tatagggttt cgggtttggt
     3061 tttgaacatt catactttaa gaatctattt aaacggagca tacaccgccc gcgcgcttct
     3121 gtacagcctc aaacgtattc cttacatttt gataataaaa gtaattttca gaaataaaat
     3181 actgtccgaa ccgtttttta gaatttgcaa aggcgattgg ggcggtacag aaaaactatt
     3241 atcccgcccg cccacttgaa atttttatgc ccaagcccta tcctgcacgc tatcgtgcca
     3301 atcccaaccg aaaaggaaaa ataatgagca gcgaattgat tgtacacacc agcgatgccg
     3361 cttttgaaaa agacgtttta aatgcagata tccccgtcct gctggacttt tgggctccgt
     3421 ggtgcggccc ctgcaaaatg attgccccga ttttggacga cattgccgcc gaatttgaag
     3481 gccgtctgaa agtggtcaaa atcaacatcg acgacaacga agccaccccg tcccgtttcg
     3541 gcgtgcgcgg cattccgacc ctgatggtgt tcaaaaa
//
LOCUS       DAWHXS010000148         3509 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWHXS010000148 DAWHXS010000000
VERSION     DAWHXS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.148"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..380)
                     /locus_tag="WIE22_001931"
     CDS             complement(<1..380)
                     /locus_tag="WIE22_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127949.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WIE22_001932"
     CDS             complement(438..803)
                     /locus_tag="WIE22_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127950.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WIE22_001933"
     CDS             complement(785..1330)
                     /locus_tag="WIE22_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127951.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WIE22_001934"
     CDS             complement(1493..1765)
                     /locus_tag="WIE22_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127952.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(2641..2889)
                     /locus_tag="WIE22_001935"
     CDS             complement(2641..2889)
                     /locus_tag="WIE22_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127953.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
     gene            3196..>3509
                     /locus_tag="WIE22_001936"
     CDS             3196..>3509
                     /locus_tag="WIE22_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ4127954.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VP"
BASE COUNT         1133 a          744 c          537 g         1095 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac ctccatccga aacagaaacc aatatatttt
     1621 taattaatac atagctgatt attacattta ttattcctat aatatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agaattcttc ctattttttc gaatccacta tttcttccaa agatgcagca
     1981 aacgcaggtt aagcctagac ccaatctacg tttgctgccg catctgatcc ctattgtttc
     2041 ttatccttac atcttcctgc cttgtcaatc aaataaagac agaagactat acaaaaactg
     2101 accgacatac tgaaaatacc tatccccttc catgcaatct ccgcaccatt gacccaatag
     2161 atcagactga gaaacaacaa agccacagta taaatcaacg taaaaaatat tgcgtaaagc
     2221 acaataaagg gaacttttat ctcacggttg ttttttataa tacattcagc aacttgattt
     2281 ccgaatatac ctgataaaaa atatagtatt agatcatcca tttcgtttat cttctatgtt
     2341 ttcccattgc ggcgctagaa gactgattta atgctgcacc atgcaatata gtgaattaac
     2401 aaaaaccagt acggcgttgc ttcgccttag ctcaaagaga acgattctct aaggtgttga
     2461 agcaccgagt gaatcggttc cgtactatct gtactgtctg cggcttcgtc gccttgtcct
     2521 gatttttgtt aatctactag agtttaaaca actttatttt tgattttatc ccaagtataa
     2581 acctgacaat cgtatttaaa agccggatat aagcctagct tgttcactgg tatgtatgat
     2641 ttagactaaa aagaaatcaa tcatttgctc aattttaagc gtctccccga caggtttccc
     2701 ggtctcagga tcaaactcaa gaaaacctga taaaaaatca ttaataatca ataatttttc
     2761 atcactggaa acagtatttt ttataatttt ttcccttagt acggataaag caaaattttc
     2821 taaagaacta tattgccccc catcaatttt tttagaaatt aaagcaacta tttcattttt
     2881 aatattcata aattaccctt aaatgataaa gggagcgaag cccgcttaaa acgtataact
     2941 gtatacccaa tttcagcaag cacaggttga gttgccaagc ctaacagagc ctgaatgatt
     3001 ggttataact caaccttata tctacactta ccgaaaacct caaataactt aataaaaatc
     3061 acacctaata cttactgaaa cgataaacta cattttcttt aaataaggtt ttatctgtta
     3121 tctcatttaa agacaataaa ctttggggct gtcctagata actagggaaa ttcaaattaa
     3181 gttagaatta tccctatgag aaaaagtcgt ctaagccagt ataaacaaaa taaactcatt
     3241 gagctatttg tcgcaggtgt aactgcaaga acggcagcag agttagtagg cgttaataaa
     3301 aataccgcag cctattattt tcatcgttta cgattactta tttatcaaaa cagcccacat
     3361 ttagaaatgt ttgatggcga agtagaagca gatgaaagtt attttggcgg acaacgcaaa
     3421 ggcaaacgtg gtcgcggtgc tgccggtaaa gtcgccgtat tcggtctttt gaagcgaaat
     3481 ggtaaggttt atacggttac agtaccgaa
//
LOCUS       DAWHXS010000149         3483 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWHXS010000149 DAWHXS010000000
VERSION     DAWHXS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.149"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            208..1680
                     /gene="pyk"
                     /locus_tag="WIE22_001937"
     CDS             208..1680
                     /gene="pyk"
                     /locus_tag="WIE22_001937"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ4127955.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPMILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1983..3383
                     /locus_tag="WIE22_001938"
     CDS             1983..3383
                     /locus_tag="WIE22_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ4127956.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          909 a         1046 c          880 g          648 t
ORIGIN      
        1 aaataaattt agcctaagtc ataaaaccgt gtaattttgc gccaaaaaag gcggtttgca
       61 ggtaaaatat caaactatta atttaaatca tatttttaca gaatattccg ccgcgttcat
      121 caaatggaca tcaaaccggt tccaaatttc tgtaattttg taacaaaata ccgcaaaaca
      181 cccgattgag accaaaagga ctttcatatg aaccagacaa gccgcgatct gacccgcatc
      241 agccacaaca ctaaaatcgt cgccaccctt gggccgggca gcaacaacgt agaactgttg
      301 gaagatatga tccgcgtcgg cggcctgaac gtcgtccgct tcaacttcag ccacggcacg
      361 cccgaattcc atcaggaaaa cgcccgcatc gtgcgcgagg cggcaaaacg cgccggacag
      421 gaaatcgcca tcattgccga cctgcaaggg ccgaaaatcc gcgtgggcaa aatcgccggc
      481 ggcggcattg aattgaacaa aggcgaaacg ctggtactgg atgccgcgct cgaaggcgaa
      541 ggcacgcgcg aggcggtcgg tttggactac cgcgacctgc ccgacgacgt tgccgcaggc
      601 gacgtattgt ggctggacga cggcctgctg accctgaccg tggaatccgt cgaaggcagc
      661 aggattatca caagggtgga aaacagccat gtcctgaaaa gcaacaaagg catcaacaaa
      721 cgcggcggcg ggctgtccgc aggcgcgttg accgaaaaag acttccgcga cctgaaaacc
      781 gcgattgcca tcggttgcga ctacctcgcc atcagctttg tgaaatctgc cgaagatttg
      841 cacatcgcgc gcgccaaagt cgaagaagaa atgaagggca gcactgccgt gcgccccggt
      901 ttggtttcca aaatcgaacg cgtagaagcg attgaaaacc ttgatgaaat catcctcgcc
      961 ggcgacggca ttatggttgc gcgcggcgac ttggcggtcg aagtcggaca cgccgccgtc
     1021 cccgccctgc aaaaacggat gatccgccgc gcccgcgagt tgcgccgctt cagcattacg
     1081 gcgacgcaaa tgatggaatc gatgattacc aaccccgtgc cgacccgcgc ggaagtcagc
     1141 gatgtggcaa acgcggtatt ggacggtacc gatgcggtga tgtgttccgc cgaaaccgcc
     1201 gtcggcgcgt atccgtttga aaccgtcagc caaatggcaa ttatctgcgc ggctgcggaa
     1261 aaagagcagg attcgctcaa cggcgttgcc gaacaggcgg agtatcccga agcggtcagc
     1321 accaacctgg cggttgccgg cggcgcggtc agcgtggcgc gcgcggttca cgccaaagcg
     1381 attgtcgccc tgaccgaaag cggttcgacc gcctttgaaa tcagccgcca caacatcacc
     1441 ctgccgattt tcgcgctgac cccgagcgtt tccgcccaac gccgtatggc gatgtaccgg
     1501 ggcgtgcgtc cgatgatttt ggcgaccagt accgaccacg acacggcgtt aaacgaagtc
     1561 gaaaccatgc tggtggaaca caacatcctg cattccggcg accagtacat catcaccagc
     1621 ggctcgcaaa tgcgcgaatc cggttcgacc aacacactgg aagtgctgcg cgtcaaataa
     1681 tccgccctga gtggaaaatg ccgtctgaag ccgatgcccg gggcttcaga cggcatttta
     1741 tttgcattcc gtcaggtaaa accgaaaaaa ttgtgcaaat ccggcaacat cagataaaat
     1801 tgagtaccta tactaaagcg aaacaaggca tttccgactg ccttttttat agtggattaa
     1861 atttaaacca gtacagcgtt gccttgccgt actatctgta ctgtctgcgg cttcgtcacc
     1921 ttgtcctgat ttttgttaat ccactatatt tatccaccgt ccgccttttt acggaaaccg
     1981 aaatgacccc ttccgcactg aaaaaaaccg tcctgctgct cggcactgcc tttgccgccg
     2041 catccgtcca cgcatccggc taccacttcg gcacacagtc ggtcaacgcg caaagcacgg
     2101 caaatgccgc cgccgcagaa gccgccgacg catcgaccat cttctacaac cctgccggcc
     2161 tgaccaaact cgacagcagc cagatttccg tcaacgccaa catcgtgctg cccagcattc
     2221 attatgaggc ggattccgcc accgacttta ccgggcttcc cgtccaaggt tcgaaaagcg
     2281 gcaaaatcac caaaaccacg gtcgcgcccc acatctacgg cgcatacaaa gtcaacgaca
     2341 atctgaccgt gggcttgggc gtgtacgtcc ccttcggctc cgccaccgaa tacgaaaaag
     2401 attccgtgtt gcgccacaac atcaacaaac tcggtctgac cagcatcgcc gtcgaacctg
     2461 tcgccgcatg gaaactcaac gaccgccatt ccttcggcgc aggcatcatc gcccaacata
     2521 cttccgccga actgcgcaaa tatgccgact gggggattaa gagtaaagca gagatattga
     2581 cggcaaaacc gcccaaacct aacggtgtag ccgaagctgc aaaaattcag gccgacggac
     2641 acgccgatgt caaaggcagc gattggggct tcggctacca actggcgtgg atgtgggaca
     2701 tcaacgaccg cgcgcgcgtg ggcgtgaact accgttccaa agtctcgcac acgctcaaag
     2761 gcgatgccga atgggcggca gacggcgcgg cggcgaaagc aatgtggagt acgatgcttg
     2821 cagcaaacgg ctacacggcg aatgaaaaag cccgcgttaa aatcgttacg cctgagtctt
     2881 tgtccgtaca cggtatgtac aaagtgtccg ataaagccga cctgttcggc gacgtaactt
     2941 ggacgcgcca cagccgcttc gataaggcgg aactggtttt tgaaaaagaa aaaaccatcg
     3001 tcaacggcaa atccgaccgc accaccatca cccccaactg gcgcaacacc tacaaagtcg
     3061 gcttcggcgg ttcttatcaa atcagcgaac cgctgcaact gcgcgccggc atcgcttttg
     3121 acaaatcgcc cgtccgcaac gccgactacc gcatgaacag cctgcccgac ggcaaccgca
     3181 tctggttctc cgccggcatg aaataccata tcggcaaaaa ccacgtcgtc gatgccgcct
     3241 acacccacat ccacatcaac gacaccagct accgcacggc gaaggcaagc ggcaacgatg
     3301 tggacagcaa aggcgcgtct tccgcacgtt tcaaaaacca cgccgacatc atcggcctgc
     3361 aatacaccta caaattcaaa taaacggccc gccgtttgat tgtagaaatg ccgtctgaaa
     3421 cagatttccg tttcagacgg cattgtttta ggcgcgattt atagtggatt aacaaaaatc
     3481 aga
//
LOCUS       DAWHXS010000150         3443 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWHXS010000150 DAWHXS010000000
VERSION     DAWHXS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.150"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..744
                     /locus_tag="WIE22_001939"
     CDS             <1..744
                     /locus_tag="WIE22_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4127957.1"
                     /translation="KLGVKTGLSIDKNRLPYLDEYYKTTKPAGTLFEPDYEALNAYKP
                     QLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDALAQIFGKQAEADKLKA
                     EIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWLHKDIGLPAVDEAIKEG
                     SHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNPLVAETTAWKKEQVVYL
                     VPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            762..953
                     /locus_tag="WIE22_001940"
     CDS             762..953
                     /locus_tag="WIE22_001940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127958.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFYWNLPHPLPIRRL
                     QTRLSAPTVRTTLFESSTP"
     gene            972..1940
                     /locus_tag="WIE22_001941"
     CDS             972..1940
                     /locus_tag="WIE22_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ4127959.1"
                     /translation="MTAKPFSLNLTNLLLLAALFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            1930..2904
                     /locus_tag="WIE22_001942"
     CDS             1930..2904
                     /locus_tag="WIE22_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ4127960.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2949..>3443
                     /locus_tag="WIE22_001943"
     CDS             2949..>3443
                     /locus_tag="WIE22_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127961.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGVGPAGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPE
                     MRELM"
BASE COUNT          647 a          950 c         1003 g          843 t
ORIGIN      
        1 aaattgggcg tgaaaaccgg tttatccatc gataaaaacc ggctgcctta tttggacgaa
       61 tattacaaaa ccacgaaacc tgccggaact ttgttcgagc cggattacga agccttaaac
      121 gcctacaagc cgcagctcat catcatcggc agccgcgcag ccaaggcgtt tgacaaatta
      181 aacgaaatcg cgccgaccat cgaaatgacc gccgataccg ccaatctgaa agaaagtgcc
      241 aaagagcgca tcgacgcgct ggcgcaaatc ttcggcaagc aggcggaagc ggacaagctg
      301 aaggcggaaa tcgacgcgtc ttttgaagcc gcgaaaactg ccgcacaagg caaaggcaag
      361 ggtttggtga ttttggtcaa cggcggcaag atgtccgcct tcggcccgtc ttcacgactg
      421 ggcggctggc tgcacaaaga catcggtttg cccgctgttg acgaagccat caaagaaggc
      481 agccacggtc agcctatcag ctttgaatac ttgaaagaga aaaatcccga ctggctgttc
      541 gtccttgacc gcagcgcggc catcggcgaa gagggtcagg cggcgaaaga cgtgttggat
      601 aatccgctgg ttgccgaaac aaccgcttgg aaaaaagaac aggtggttta cctcgttccc
      661 gaaacttatt tggcagccgg tggcgcgcaa gagctgctga atgcaagcaa acaggttgcg
      721 gatgcgttca acgcggcaaa ataatgaaac ggcggcattc gatgccgtct gaaacacgga
      781 tgcaaaccgc ttcctgtgtt tcagacggca ttgcccgata cgggcgttat cgcgggaatg
      841 gcggcaagcg tttttattgg aacttgcccc atcctttgcc catacggagg cttcaaacaa
      901 ggctttccgc tccgacggtt cggactacct tgtttgaatc ttctacgcct taacgctttt
      961 cccttctgtt tatgactgcc aaaccttttt ccctcaacct gaccaacctc ctgctgctgg
     1021 cggcgttgtt tgccgtcagc ctgtcggtgg gcgttgccga tttccgctgg tcggatgtgt
     1081 tttcgctatc cgacagccag caagtgatgt tcatcagccg cctgccgcgc acgtttgcga
     1141 ttgtgctgac gggcgcgtcg atggcggtgg cggggatgat tatgcagatt ttgatgcgca
     1201 accgttttgt cgagccttct atggcgggcg cgggtcaaag tgcggctttg ggtttgcttc
     1261 tgatgtccct gctgctgcct gccgcgccgc tgccggtcaa aatgtcggtt gccgccgttg
     1321 ccgcgttaat cgggatgttg gtgtttatga tgcttatccg ccgcctgccg ccgacggcgc
     1381 aactgatggt gccgctggtg gggattattt tcggcggcgt ggttgaggcg gtggcgacgt
     1441 ttatcgcgta tgaaaacgaa atgctgcaaa tgctcggcgt gtggcagcag ggcgattttt
     1501 cgagcgtgct gctggggcgg tacgagctgc tttggattac gggcggtttg gcagtgtttg
     1561 cctatctgat tgccgaccgg ctgacgattt tggggctggg cgaaacggtc agcgtgaatt
     1621 tgggtttgaa ccggacggcg gtgttgtggt cggggctgat tattgtggct ttgattacgt
     1681 cgctggttat cgttacggtc ggcaatattc cgtttatcgg gctggtcgtg ccgaacatca
     1741 tcagccgcct gataggcgac aggctgcgcc aaagcctgcc tgcggtggcc ttgttgggcg
     1801 cgtctttggt tttattgtgc gacattatcg gacgcgtgat tgtgtttccg tttgaaattc
     1861 cggtctctac ggtttttggt gtgttgggta cggctttgtt tttgtggctt ttgttaagga
     1921 aacctgctca tgccgtctga aaaaaatatc ggttttatgg caggaagcag ccgtccgttg
     1981 tgggttgcct ttgcgctgtt gctggtttcc tgcatcctgt ttatgacgct caacgtcaaa
     2041 ggcgattggg actttgtact gcacctgcgt ctgaccaaac ttgccgcgct gctgatggtc
     2101 gcctatgcgg tcggcgtgtc cacgcaactc ttccaaacgc tgaccaacaa tccgattctg
     2161 accccttcaa ttttgggttt cgattcgctg tatgtgtttt tgcagacctt gctggtgttt
     2221 acgttcggcg gcgtgggcta tgcttccctg ccgttgacgg gcaaattcgg ctttgaactt
     2281 gccgttatga tgggcggctc gctgctgctg ttttacacgc tcatccgtca gggcggacgc
     2341 gatttgccgc gtatgatttt aatcggcgtg attttcggga ttttgttccg cagcctgtcg
     2401 tcgctgcttt cgcgcatgat cgaccccgaa gaatttacgg cggcgcaggc gaatatgttt
     2461 gccggattca ataccgtcca cagcgagctt ttgggcatag gcgcgctgat tctgctcgtc
     2521 agcgcggcgg tcgtttggcg cgaacgctac cgcttggacg tacacctttt ggggcgcgac
     2581 caagccgtca atttgggcat cagctacacg cgcaacacct tgtggatact gctttggatt
     2641 gccgcattgg tggcgacggc gaccgccgtg gtcggcccgg taagcttttt cgggcttctc
     2701 gccgcctcgc ttgccaacca cttttccccg tccgtgcgcc attccgtccg cctgccgatg
     2761 acggtttgtg tcggcggcat cctcctggtc ggcggacaga ccgtattcga acacttcttg
     2821 ggcatgaagg cggtattaag cgtggtggtc gaatttgcgg gcggactcgt tttcctctat
     2881 ctcgttttaa aacacaaaaa atgacggatg ccatctgaac ggcagcccgc cccgaaagga
     2941 caaaccatat gacgcaagaa cgtttacccg aatttttcga ccgcgccccg acgctgaccg
     3001 tacaagaccc gcttgccgca ttcctcggcg cggccgaaaa cggcatcctc acttaccgct
     3061 acgccgatgc cgtgcgcctg tgcggacatt cctgcccgac cgtcgcgggc gcgtacctga
     3121 tggttatcaa aggtctgaaa gcactttacg gcgaagagct gcccgaacgc ggcggcatcg
     3181 aagcctttat gcagggcgcg cgcgacgaag gcacggtcgg cgtaaccgcg tccgtcgtcc
     3241 aactccttac cggcgccgca cccgaaaccg gctttggcgg cgtaggccct gccggacgct
     3301 ttgcccgccg caacctcttg tcctttggtg caggcgaaat caacggcaca ctcgcgctcc
     3361 gccgccgcga taccggcaaa accgtcgccg tcagcctcaa cgccgccctg caacccttcg
     3421 cacccgaaat gcgcgaactt atg
//
LOCUS       DAWHXS010000151         3304 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWHXS010000151 DAWHXS010000000
VERSION     DAWHXS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.151"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            418..741
                     /locus_tag="WIE22_001944"
     CDS             418..741
                     /locus_tag="WIE22_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ4127962.1"
                     /translation="MQNETINPKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            855..3032
                     /locus_tag="WIE22_001945"
     CDS             855..3032
                     /locus_tag="WIE22_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ4127963.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLTGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGIRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
     gene            3125..>3304
                     /locus_tag="WIE22_001946"
     CDS             3125..>3304
                     /locus_tag="WIE22_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ4127964.1"
                     /translation="MSYTQLTQGERYHIQYLSRHCTVTEIAKQLNRHKSTISREIRRH
                     RTQGQQYSAEKAQRQS"
BASE COUNT          811 a         1157 c          831 g          505 t
ORIGIN      
        1 gccctctccc acagggagag gggattgggg tgcaggctgt ctttaagctt caggcgattt
       61 ttaactcttg ttgaagctgc gatttcagaa gctccgtttt agcttcgcag aaactccgct
      121 tccttcgaaa gctccgtttt cagacgacct tttggaacac cacaggcaca cgcatcgaac
      181 ggctgaatca aagttcagac cgatggcagc ccgcacccga gtttatgcgg caaacagcga
      241 ggctacggca acccgtcccc tctccctgtg ggagagggtt agggagaggg cggtaagccg
      301 caggcttaca tcaaagccga taacgcttcc gttacaactc cgcccactga aagcagcctg
      361 caacgaagcc aaaacgacaa accgcatcgt aaaccaccca acccatagga gaaccccatg
      421 caaaacgaaa ccatcaaccc gaaacagcac cttgccgcca tcaaagaata ctggcagccc
      481 gaaatcatca accgccacgg gttccaattc cgcttggtca aacttttggg cgattacggc
      541 tggcatacgc acggatacag cgacaaagtg ctgtttgccg tggagggcga catggcggtg
      601 gacttcgccg acggcggcag catgacgata cgcgagggcg agatggcggt cgtgccgaag
      661 tcggtgtcgc accgcccgcg ttcggaaaac ggctgctcgg tggtgctgat tgagttgtcc
      721 gacccgtccg aggccgtctg aaaacgaagt ttccgaagga agctgagttt ctgcgaagct
      781 aaaagcagcc tgcaccttca atcaatatgc cgaaaataca accccaccgc acaccaacac
      841 acaaaggaaa tcccatgaca cgcttcaaat attccctgct gtttgccgcc ctgttgcccg
      901 tgtacgcgca ggccgatgtt tctgtttcag acgaccccaa accgcaggaa agcactgaat
      961 tgccgaccat caccgttacc gccgaccgca ccgcgagttc caacgacggc tacactgttt
     1021 ccggcacgca caccccgctc gggctgccca tgaccctgcg cgaaatcccg cagagcgtca
     1081 gcgtcatcac atcgcaacaa atgcgcgacc aaaacatcaa agcgctcgac cgcgccctgt
     1141 tgcaggcgac cggcaccagc cgccagattt acggctccga ccgcgcgggc tacaactacc
     1201 tgttcgcgcg cggcagccgc atcgccaact accaaatcaa cggcatcccc gttgccgacg
     1261 cgctggccga tacgggcaat gccaacaccg ccgcctatga gcgcgtagaa gtcgtgcgcg
     1321 gcgtggcggg gctgctggac ggcacgggcg agccttccgc caccgtcaat ctggtgcgca
     1381 aacgcccgac ccgcaagcca ttgtttgaag tccgcgccga agcgggcaac cgcaaacatt
     1441 tcgggctggg cgcggacgta tcgggcagcc tgaatgccga aggcacgctg cgcggccgcc
     1501 tggtttccac cttcggacgc ggcgactcgt ggcggcagcg cgaacgcagc cgcgatgccg
     1561 aactctacgg cattttggaa tacgacatcg caccgcaaac ccgcgtccac gcaggcatgg
     1621 actaccagca ggcgaaagaa accgccgacg cgccgctcag ctacgccgtg tacgacagcc
     1681 aaggttatgc caccgccttc ggcccgaaag acaaccccgc cacaaattgg gcgaacagcc
     1741 gccaccgtgc gctcaacctg ttcgccggca tcgaacaccg cttcaaccaa gactggaaac
     1801 tcaaagccga atacgactac acccgcagcc gcttccgcca gccctacggc gtagcaggcg
     1861 tgctttccat cgaccacaac accgccgcca ccgacctgat tcccggttat tggcacgccg
     1921 acccgcgcac ccacagcgcc agcgtgtcat tgaccggcaa ataccgcctg ttcggccgcg
     1981 aacacgattt aatcgcgggt atcaacggtt acaaatacgc cagcaacaaa tacggcgaac
     2041 gcagcatcat ccccaacgcc attcccaacg cctacgaatt ttcccgcacg ggcgcctatc
     2101 cgcagccatc atcgtttgcc caaaccatcc cgcaatacgg catcaggcgg caaatcggcg
     2161 gctatctcgc cacccgtttc cgcgccgccg acaacctttc gctgatactc ggcggcagat
     2221 acagccgtta ccgcaccggc agctacgaca gccgcacaca aggcatgacc tatgtgtccg
     2281 ccaaccgttt caccccctac acaggcatcg tgttcgacct gaccggcaac ctgtctcttt
     2341 acggctcgta cagcagcctg ttcgtcccgc aatcgcaaaa agacgaacac ggcagctacc
     2401 tgaaacccgt aaccggcaac aatctggaag ccggcatcaa aggcgaatgg cttgaaggcc
     2461 gtctgaacgc atccgccgcc gtgtaccgcg cccgtaaaaa caacctcgcc accgcagcag
     2521 gacgcgaccc gagcggcaac acctactacc gcgccgccaa ccaagccaaa acccacggct
     2581 gggaaatcga agtcggcggc cgcatcacgc ccgaatggca gatacaggca ggttacagcc
     2641 aaagcaaaac ccgcgaccaa gacggcagcc gcctgaaccc cgacagcgta cccgaacgca
     2701 gcttcaaact cttcactgcc taccactttg cccccgaagc ccccagcggc tggaccatcg
     2761 gcgcaggcgt gcgctggcag agcgaaaccc acaccgaccc tgccacgctc cgcatcccca
     2821 accccgccgc caaagcccgc gccgccgaca acagccgcca aaaagcctac gccgtcgccg
     2881 acatcatggc gcgttaccgc ttcaatccgc gcgccgaact gtcgctgaac gtggacaatc
     2941 tgttcaacaa acactaccgc acccagcccg accgccacag ctacggcgca ctgcggacag
     3001 tgaacgcggc gtttacctat cggtttaaat aaggtcggcg gattcgcatt tgaagtgcaa
     3061 ctttccctaa cagaaaaagg ccagtatgcg gtagcatacg gcctttcctg caagaaagat
     3121 tgccatgagc tacacgcaac tgacccaggg cgaacgatac cacatccaat acctgtcccg
     3181 ccactgcacc gtcaccgaaa tcgccaaaca gctgaaccgc cacaaaagca ccatcagccg
     3241 cgaaatcaga cggcaccgca cccaagggca gcaatacagc gccgaaaaag cccagcggca
     3301 aagc
//
LOCUS       DAWHXS010000152         3211 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWHXS010000152 DAWHXS010000000
VERSION     DAWHXS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.152"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            167..820
                     /locus_tag="WIE22_001947"
     CDS             167..820
                     /locus_tag="WIE22_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ4127965.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            899..1552
                     /locus_tag="WIE22_001948"
     CDS             899..1552
                     /locus_tag="WIE22_001948"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ4127966.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1656..2555
                     /locus_tag="WIE22_001949"
     CDS             1656..2555
                     /locus_tag="WIE22_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ4127967.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2552..>3211
                     /locus_tag="WIE22_001950"
     CDS             2552..>3211
                     /locus_tag="WIE22_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ4127968.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AA"
BASE COUNT          862 a          850 c          792 g          707 t
ORIGIN      
        1 tttaatctta ctgttctttc cgctccaaag attctgtatg attcggcaat tcctgccgtg
       61 cagacaacgt aaaaaaatac tacattaaat ctgccaaatg cgttaagatg aaaatattca
      121 aattccgtac gaatcaggtt ttgctattta ttcttgggag attgtcatgt tttccgtacc
      181 gcgttccttt ttgccgggcg ttttcgtact tgccgcgctt gccgcctgca aacctcaaga
      241 caacagtgcg gcgcaagccg cttcttcaag tgcatccgcg tcagctgcgg aaaatgcggc
      301 aaagccgcaa acgcgcggta cggatatgcg taaggaagac atcggcggcg atttcacgct
      361 gaccgacggc gaaggcaagc ctttcaacct gagcgatttg aaaggcaagg tcgtgattct
      421 gtctttcggc tttacgcact gtcccgatgt ctgcccgaca gagcttttga cgtacagcga
      481 cacgttgaag cagttgggcg ggcaggctaa ggacgtgaaa gtggtgttcg tcagcatcga
      541 tccggaacgc gacacgcctg aaatcatcgg caagtatgcc aaacagttca atccggactt
      601 tatcggtctg acggcaacgg gcggccaaaa cctgccggtc atcaagcagc aataccgcgt
      661 ggtttctgcc aaagtcaatc aaaaagacga cagcgaaaac tatttggtcg accactcttc
      721 cggtgcgtat ctcatcgaca aaaacggtga ggttgccatt ttctcgcctt acggaagcga
      781 gccggaaacg attgctgccg atgtaaggac cctgctctga taaaaccgta tgccgtctgc
      841 accgtcggcg cctattcaga cggcattatt gtttcaaccg acaaaggaca tccacaccat
      901 gcaggataat gctttgacca tcgccttatc caaggggcgc atttttgagg agacgctgcc
      961 gctgcttgcc gctgccggca ttgttccgac tgaagagcct gaaaaatcgc gcaagctgat
     1021 tatcgggacg aaccatgaaa acatccgcct tgtcattgtc cgcgcaaccg atgtgccgac
     1081 ttatgtccgc tacggcgcgg cggacttcgg cattgcgggc aaagacgtgc tgatcgaaca
     1141 cggcggcacg gggctttacc ggcctttgga tttggagatt gccaagtgcc gcatgatggt
     1201 tgctgtgcgt aaagggtttg attacgaagc agcttcgcaa cccggatgcc gtctgaagat
     1261 tgccacgaag tatcctgaaa tcgcggcatc tcattttgcc ggcaagggtg tccatgtgga
     1321 cattatcaaa ctgtacggct cgatggaact tgcgccgctg gtcggcttga gcgatgcgat
     1381 tgtggacttg gtttcgacgg gcaacacctt gaaggcaaac ggcttggaag cagtcgaaca
     1441 catcgtcgac atttccagcc gcctggtggt caacaaggct gctttgaaaa cgaaatacgc
     1501 gctgctggag ccgattattc aggcgttcgg cggcgcagtg aaggcgaagt aagcatccat
     1561 ttgaataaag atgcgttttc agacgaccct atccgttccc gccgacaggt cgtctgaaaa
     1621 tatcaccggc agtaaactgt ataggagaag ttaaaatggt tgcaaaaata aaaaaattct
     1681 cagattcaac cctttccgtt ttgaataacg gcgagcgtcg gttttatgtc tattgtctga
     1741 ccgacctgaa aaaagacaaa atcctctaca tcggcaaagg ctgcggtaat cgtatcttcg
     1801 agcatgaatg ggttgctagt cgttcacaag acccagtctc cggcgagatt atcgatcgga
     1861 aactcaaagc catctccaaa tgcaagaaac tcggtcgcta tatcatcagc tatcatctga
     1921 ctgaagtcga agcactcgcc gccgaatctg ccttaattca ttttgttaaa tctgtcttgg
     1981 gtaaaaaact caaaaataaa attgccgggc atggtccggg tggtattagc gtagaagaac
     2041 tagatcgccg ctttggattc tcttctctcc cacttaacga gattaacccc gacgggctga
     2101 ttctcgccat caaaatccac aatgctttcg atttagatac tgacgaagaa ttagactacc
     2161 ttttcgacaa ccaagacgat gccaacctca aatcgcgtac gttgggcaac tgggttatcg
     2221 gtaaagatgt tgcttcaaaa gtgaaatacg ttatcggcgt tcacaccggt ctgcaaaacg
     2281 ctgttgtcag tgcatacgaa gtggacggtt ttgaaacaat ggttgaggaa accaaaaacg
     2341 gtagaaaaca atcccgttac cgtttccgca ctacctctcg tagcgaagag gtattagcca
     2401 aactcggtct gcaacaaaaa tgcctgcccg aattgaagtt tggtagcggg ggagaaaaag
     2461 cgtatatcag acccaaaaca gagacagaaa ctgaacaaga gaatattcag acgaccccca
     2521 atccaaaaat aaaaaaggaa aaaaccaaat catgaaaaaa ctcaacaccc aatcgcccga
     2581 tttccaagcc ggactcaaag ccctgctggc ttttgaaacc gcgcaaaacc ccgaaaccga
     2641 acgcatcgtc gccgacattt gcgccgacgt gcaaaagcgc ggcgatgcgg ctttgattga
     2701 atacaccaac aaattcgatc agacaaacgc taaaagcatc gatgatttaa tactcacgca
     2761 agccgatttg aacgcggcgt tcgagcgcat tccgaacgac gttcagacgg cattgcagac
     2821 cgccgcccgc cgtgtcgaaa gctaccacca acgccaaaaa atggaatcgt ggagctacac
     2881 cgatgaagac ggcacgctgt tgggacaaca gattaccccg cttgaccgcg tcggcattta
     2941 cgtccccggc ggcaaggcgg catatccgag ttccgtcatc atgaacgcca tgcccgccca
     3001 cgtcgcaggc gtgaaagaaa tcatcatggt cgtgccgaca ccgaaaggcg aacgcaacga
     3061 catcgtgctt gccgccgcat acgtcgccgg cgtaaccaaa gtcttcaccg tcggcggcgc
     3121 gcaggcggtt gccgccctcg cctacggcac ggaaaccatc ccccaagtcg ataaaatcac
     3181 cggtccgggc aacgccttcg tcgccgccgc c
//
LOCUS       DAWHXS010000153         3056 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWHXS010000153 DAWHXS010000000
VERSION     DAWHXS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.153"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            110..2878
                     /locus_tag="WIE22_001951"
     CDS             110..2878
                     /locus_tag="WIE22_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4127969.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRTGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTRDVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            2908..>3056
                     /locus_tag="WIE22_001952"
     CDS             2908..>3056
                     /locus_tag="WIE22_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127970.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFG"
BASE COUNT          872 a          767 c          734 g          683 t
ORIGIN      
        1 attgaaatct tcagacggta tatcgaattt acacttttta atgtttatgc cgcctaaaaa
       61 aatgctaata tatttcttaa ttgtctgact gtttattgtt gaggaaaata tgagatcttc
      121 tttccggttg aagccgattt gtttttatct tatgggtgtt atgctatatc atcatagtta
      181 tgccgaagat gcagggcgca cgggcagcga ggcgcagata caggttttgg aagatgtgca
      241 cgtcaaggcg aagcgcgtac cgaaagacaa aaaagtgttt accgatgcgc gtgccgtatc
      301 gacccgtcag gatatattca aatccagcga aaacctcgac aacatcgtac gcagcatacc
      361 cggtgcgttt acacagcaag ataaaagctc gggcattgtg tctttgaata ttcgcggcga
      421 cagcgggttc gggcgggtca atacgatggt ggacggtatc acgcagacct tttattcgac
      481 ttctaccgat gcgggcaggg caggcggttc atctcaattc ggtgcatctg tcgacagcaa
      541 ttttattgcc ggactggatg tcgtcaaagg cagcttcagc ggctcggcag gcatcaacag
      601 ccttgccggt tcggcgaatc tgcggacttt aggcgtggat gacgtcgttc agggcaataa
      661 tacctacggc ctgctgctaa aaggtctgac cggcaccaat tcaaccaaag gtaatgcgat
      721 ggcggcgata ggtgcgcgca aatggctgga aagcggagca tctgtcggtg tgctttacgg
      781 gcacagcagg cgcagcgtgg cgcaaaatta ccgcgtgggc ggcggcgggc agcacatcgg
      841 aaattttggc gcggaatatc tggaacggcg caagcagcaa tattttgtac aaacaggcgg
      901 gttgaaattc aattccaaca gcggaaaatg ggaacgcgat ttgcaaaggc aattctggaa
      961 aaccagttac tataaacaat acaaagaccc ccagaaactg aaaaaataca tcgaagacca
     1021 agataaagac tggcgggaaa acctggcgcc gcaatacgac atcaccccca tcgatccgtc
     1081 cagcctgaag cagcagtcgg caggcaatct gtttaaattg gaatacgacg gcgtattcaa
     1141 taaatacacg gcgcaatttc gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa
     1201 ccgcaattat caattcaatt acggtttatc tttaaactca tatgccaacc tcaatctgac
     1261 cgcagcctac aattcgggca ggcagaaata tccgaaaggg tcgaagttta caggctgggg
     1321 gcttttaaag gattttgaaa cctacaacaa cgcaaaaatc ctcgacctca acaacaccgc
     1381 caccttccgg ctgccccgcg aaaccgagtt gcaaaccact ttgggcttca attatttcca
     1441 caacgaatac ggcaaaaacc gctttcctga agaattgggg ctgtttttcg acggtcctga
     1501 tcaggacaac gggctttatt cctatttggg gcggtttaag ggcgataaag ggctgctgcc
     1561 ccaaaaatca accattgtcc aaccggccgg cagccaatat ttcaacacgt tctacttcga
     1621 tgccgcgctc aaaaaagaca tttaccgctt aaactacagc accaataccg tcggctaccg
     1681 tttcggcggc gaatatacgg gctattacag ctcggaaggc gaatttaagc gggcattcgg
     1741 agaaaactcg ccgacataca agaaacattg caacccgagc tgcgggcttt atgaacccgt
     1801 attgaaaaaa tacggcaaaa agcgcgccaa caaccattcg gtcagcatta gtgcggactt
     1861 cggcgattat ttcatgccgt tcgccagcta ttcgcgcaca caccgtatgc ccaacatcca
     1921 agaaatgtat ttttcccaaa tcggcgactc cggcgttcac accgccttaa aaccagagcg
     1981 cgcaaacact tggcaatttg gcttcaatac ctataaaaaa ggactgttaa aacaagatga
     2041 tacattagga ttaaaactgg tcggctaccg cagccgcatc gacaactaca tccacaacgt
     2101 ttacgggaaa tggtgggatt tgaacgggaa tattccgagc tgggtcagca gcaccgggct
     2161 tgcctacacc atccaacacc gcaatttcaa agacaaagtg cacaaacacg gttttgagtt
     2221 ggagctgaat tacgattatg ggcgtttttt caccaacctt tcttacgcct atcaaaaaag
     2281 cacgcaaccg accaacttca gcgatgcgag cgaatcgccc aacaatgcgt ccaaagaaga
     2341 ccaactcaaa caaggttatg ggttgagcag ggtttccgcc ctgccgcgag attacggacg
     2401 tttggaagtc ggtacgcgct ggttgggcaa caaactgact ttgggcggcg cgatgcgcta
     2461 tttcggcaag agcatccgcg cgacggctga agaacgctat atcgacggca ccaacggggg
     2521 aaatacccgc gatgtccggc aactgggcaa gcgttccatc aaacaaaccg aaacccttgc
     2581 ccgccagcct ttgatttttg atttttacgc cgcttacgag ccgaagaaaa accttatttt
     2641 ccgcgccgaa gtcaaaaatc tgttcgacag gcgttatatc gatccgctcg atgcgggcaa
     2701 tgatgcggca acgcagcgtt attacagctc gttcgacccg aaagacaagg acgaagaagt
     2761 aacgtgtaat gatgataaaa cgttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgatgacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtagaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaatc cgttttatcg catcggcaac
     3001 tcaaagaaaa atccatttca ttcccacgca gggaagccgg tttttgattt cggtta
//
LOCUS       DAWHXS010000154         2886 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWHXS010000154 DAWHXS010000000
VERSION     DAWHXS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.154"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            35..1252
                     /locus_tag="WIE22_001953"
     CDS             35..1252
                     /locus_tag="WIE22_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4127971.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTAIAIIGLANIAGCVF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1455..1595)
                     /locus_tag="WIE22_001954"
     CDS             complement(1455..1595)
                     /locus_tag="WIE22_001954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4127972.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1608..2732
                     /locus_tag="WIE22_001955"
     CDS             1608..2732
                     /locus_tag="WIE22_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ4127973.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGDLERLAARDIIRFPGFVNNVSEVIKAHH
                     IFVLPSYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKM
                     IYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          679 a          862 c          678 g          667 t
ORIGIN      
        1 aatccgcaaa cttaaccgac tcaaaataca cattatgacg cacaccgcat cgaaaacacc
       61 caaattttgg ctgaccatca ccgccgccgc attcatcctg ctgattacca tcggcatgag
      121 gatgacgctc ggactgttcg tccaacccgt cgtcaacacc accgaattga gcattgccca
      181 attcagcctc atcatcgccg ttttccaact gatgtggggc atatcgcaac cattgtccgg
      241 cgcgcttgcc gaccgtttcg gcgcgttcag ggtattaagc ggcggcgccg tcctgctcgt
      301 ctgcgcctgc ctgattgccc ccaacatccc gacttattgg gggctgatga ttgccgtcgg
      361 gctgctgctc gcattcggca ccggttccgg cggtttctcc atcattatgg ggcaggtcgc
      421 cgcccaagtc cccgcacaca aacgcggctt ggcatccgga ctggtcaacg ccggcggttc
      481 ggcaggacaa ttcctgttcg caccgctggt tcaaggactc gtcgtcctgc ccgaagtcgg
      541 ctggacgggc acattttacg tttggggcgc aatcgccctg ctgattctgc ctgtctcatg
      601 gtggcttgcc ggcggcaaca acgccgccca cacccaacac gcccaagcca cacacggaca
      661 aagcctcgga gaagcagtca aaaccgcctt caaaacccca agctacatcc tgctgcacct
      721 aagctttttt gcctgcggct tccacatcgc ctttctcgta acccacctac ccacggaagt
      781 cgccctgtgc ggactgcccg ccaccgtcgc atccacagcc atcgccatca tcggactggc
      841 aaacatcgcc ggttgtgtgt tctcaggctg gtgcacaagc cgcgttcggg gcaaatacgt
      901 cctgttcgga ctgtacgcct cgcgcgccgc gatgatactc atctacattt tttcgcccaa
      961 gaccgacctc aacttctaca ttttcgccgc cgcactcgga tttacctggc tcgccaccgt
     1021 cacgccgacc gcctccatta ccggcaaact cttcggcaca cgctacctcg ccaccctgtt
     1081 cggactgacg atgctcagcc accaaatcgg cggattcctc ggctcgtaca tcggcggcat
     1141 cgtgattaca caattcggcg actacggctg gatgtggtac gccgacgcac tgctggcggg
     1201 aaccgccgcc ctcctcaacc tccccatccg cgaaccccga accgccgcat gaaagaatgc
     1261 cgtctgaaaa ccctcagacg gcattttcca aacaccccgc ccgtttccgg actgcacgac
     1321 acgcaaatca gccttatcaa accgcaaaca accgccgcga ttaaaccgcc attcaaataa
     1381 gtccgttttc ccttcgggtg taaaatcatc acaaaacacg atactgtata tcgccgggcg
     1441 ttatgcagcg gatttcagga taaaatgccg tctgaagccg cccctgcccg ggcgggcgaa
     1501 gcgtcggatg cggtgcgctt ggcaaaggtt tgcccgccgc gccccgtatc cggcatttca
     1561 tcaatgcaga ccgcatttcc aatcctataa ttcaaggtgt ccgccttatg aaaatcgttt
     1621 ttatcacaac agtcgcatcc agcatttacg gtttccgcgc ccccgtcatt aaaaaattaa
     1681 tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt ttccgataat gaattggata
     1741 ttatcaggga aatgggggtt acacccgtta cctaccgttc aaaccgcagc gggctgaacc
     1801 cgttttccga tataaaatcc accttcctca tatttaaaga actcaaaaaa atatcgccgg
     1861 atttggtttt cccttatttc gcaaaacccg tgattttcgg cacttttgcc gcaaaactgg
     1921 caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg attcgcattt accccgcagc
     1981 cggaaggcat accgttaaaa acaaaaatca taaaggggat tttgattgcc ttgtaccaca
     2041 ttgccctgcc gatgttggaa agcctgattg tattaaaccc cgacgacaaa gacgaactga
     2101 cggacaaata cggcatcaaa ataaaaaaca tccatatttt gggcggaatc ggtctggatt
     2161 cgcggcaata tccttattcc gaggcggata ttcccgatga aaaagaaccc gtaaaattcc
     2221 tctttatcgg cagatttctg aaagaaaagg ggattgatga ttttattcgg gcggcggaac
     2281 aggttaagga caaatacccc gatacggttt ttaccgcttt gggcgcaatc gacaaatcac
     2341 gcgggggggg cgatttggaa cggcttgccg cccgcgatat tatccgtttc cccggttttg
     2401 tgaacaatgt ttccgaagtg ataaaggcgc atcatatatt cgtattgccg tcttataggg
     2461 aaggcgttcc ccgaagcact caggaggcaa tggccgtcgg cagggcagtg attacgacgg
     2521 atgtccccgg atgcagggaa acggtcgccg acaaggtcaa cggcttcctg atcgagcctt
     2581 ggaatccccg catcttagcc gaaaaaatga tttattttat cgaaaacagg gaagccgtcc
     2641 gcctgatggg gaatgcaagt tatgcgattg ccaaagataa attcgatgcc gaaaaagtcg
     2701 atttgaaatt gctcgatatt ttgaaggcgt aaacaaggct gcccgctttt gagtttcggt
     2761 catttctgat aaacccccgt cattcccgcc acacccccgt cattccctcg aaagcgggaa
     2821 ttcaggttcg tttagtttcg gtcatttccg ataaattctt gcaactttgc gtttctagat
     2881 tcccac
//
LOCUS       DAWHXS010000155         2846 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWHXS010000155 DAWHXS010000000
VERSION     DAWHXS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.155"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(53..529)
                     /gene="greA"
                     /locus_tag="WIE22_001956"
     CDS             complement(53..529)
                     /gene="greA"
                     /locus_tag="WIE22_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ4127974.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            921..2222
                     /gene="aroA"
                     /locus_tag="WIE22_001957"
     CDS             921..2222
                     /gene="aroA"
                     /locus_tag="WIE22_001957"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ4127975.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIATTPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(2265..2798)
                     /locus_tag="WIE22_001958"
     CDS             complement(2265..2798)
                     /locus_tag="WIE22_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ4127976.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          601 a          784 c          767 g          694 t
ORIGIN      
        1 ttaatccact ataatttcct gcgtgtgtcg ggtgtatcga aatcaagccg aatcaaatat
       61 atcggacttc gataatgtcg tattcgcgca cgccgcccgg ggcttggact tccgccgtat
      121 cgccctcttc cttgccgatt aaggcgcggg caatcggtga gccgacatag attttgccct
      181 gtttgatatc cgcttcgtct tcgccgacaa tttggtaggt aacgtgttct tccgtttcca
      241 aatcttccag cgtaaccgtc gtaccgaaca cgattttgcc ttcggcgtgg atttcggtcg
      301 gattgatgat gtgggcaacg gaaagtttgt gttccagctc ggaaatgcgg ccttcgataa
      361 agccttggcg ttctttggcg gcttcatatt cggcgttttc ggacaaatcg ccgtgcgatc
      421 tggcttcagc aatcgcttcg atcacttcgg ggcgcgccac gcttttaagc tgctgcaatt
      481 cctgtttcag caattccgca ccgcgtacgg tcagggggat tttttgcatc ggtctttttt
      541 ctccatattc cggcacaccg gtttgcggca gcaaacatac cgcataccgt cttgttttgt
      601 gcgtccggat attaaaataa aaacacaagc cgcccggaaa atcggcggct tgtctgtcgt
      661 tgaacagcgg ctattctacc aaattctatg aaattggcaa tcgtgccgtg ccgtccgcaa
      721 acacgctatg tccgcaacaa aagccgaaaa tatgccggca aaaaaatttt agaaacaaaa
      781 aatttaaaaa taatcaattt tcggcacaaa aaaccatatt cacggacttt aaaaccgaaa
      841 atgccaaacc tgagattttt cagacggcat ttgcaccagt ataatgcagg ctgtttttat
      901 ctttatattt acgttttgcc atgaccgaat ccctccgcct ccctgccgcc tcgctcaaac
      961 cttccactgt cgccctgccc ggctccaaaa gcatcagcaa ccgcaccctg ctgcttgccg
     1021 ccttgtccga caatgcttgc gaaatccatt ccctgctcaa atccgacgat accgaccgta
     1081 tgctcgaagc actcgataaa ctcggcgttg aaatcgaata tcttgccgaa gaccgtctga
     1141 aagtgcacgg cacaggcgga cgcttcccca accgcactgc cgatttgttt ttgggcaacg
     1201 cgggcacggc gttccgcccg ctgaccgccg cgcttgccgt tttgggcggc gattatcgcc
     1261 tgcacggcgt tgcgcgtatg cacgaacgcc ccatcggcga tttggccgat gcactgcgga
     1321 ttgccggcgc cgatgtcgaa tatctcggca aggaacacta tccgccgctt catatcggcg
     1381 aacgccaaga caacggcgag cgcgtgattc cgattaaagg caatgtatcc agccagtttc
     1441 tgaccgccct tttaatggcg ttgccgctga ccgggcaggc gtttgaaatc cgtatggtcg
     1501 gcgagttaat ttccaagccc tatatcgaca ttactttaaa actgatggcg caattcggcg
     1561 tacaggttat caatgaaggc taccgcgtct tcaaaattcc cgccgatgcg cactatcacg
     1621 cgcccgaaca cttgcacgtc gaaggcgatg cctccagcgc gtcctacttc ctcgcagccg
     1681 gtttgattgc caccacgccc gtccgcgtta ccggtatcgg cgcaaacagc atacagggcg
     1741 atgtcgcctt tgcctgcgag ctggaaaaaa tcggtgcgga cgtggtttgg ggcgaaaact
     1801 tcgtcgaagt ctcgcgcccg aaagaacgtg ccgtccaagc ctttgatttg gatgcaaacc
     1861 atatccccga tgccgccatg accctcgccg tcgtcgcgct tgctacaggg caaacctgca
     1921 cgcttcgcaa catcggttcg tggcgcgtca aagagaccga ccgcattgcc gcaatggcga
     1981 acgagttgcg caaactcggt gcaaaagtcg ccgaagaagc cgaagcaatt cacatcaccc
     2041 cgcccgaaac gccgacaccc gacgccgtca tcgacactta cgacgaccac cgcatggcaa
     2101 tgtgtttctc gctggtttcg ctgttggacg tacccgtcgt catcaacgat ccgaaatgca
     2161 cccacaaaac cttcccgact tattttgaag tgttctcatc gctgaccgaa acagcggaat
     2221 aaggcggcat tttgccgtga ttccggcgca gcgcggcggg cggctcattc tgtaaaaaaa
     2281 gtatgtgcgc cgaggtagtt tttggcgtaa aacggtgtgg agagtttttc ggttttgatg
     2341 gttttgccgc tgctgggggc atggatgaat tcgccgttgc cgatatagag tccgacgtgt
     2401 gagtagcggt gtgtgccgcc ggtgttgaag aatacgaggt cgccggcctt aaggcggctg
     2461 tcggggattt tgcggcttgc cgccgccatg tcgcgggcgg tgcgcggcag cttgacgttg
     2521 agggcgtttt tgtaaacgaa ttgaatcatg ccgctgcaat cgaagccggt tgcggtgctg
     2581 ctgccgcccc atttgtaggg cgtgccgatg agtccgaggc tgtggagcat gagttcctgc
     2641 gagccttgtg tgcggtcgat gtggctgatg cggacggctt ggatttgccg gactgtctgt
     2701 ttgggtttcg gttggcggtg tttgccggag gtcgtgccgc atgaggcgag gagcagtgcg
     2761 ctgagacaga ggaaaagggt tttgtcgggg ggaaacatgg tttttccttt gcgggttcgg
     2821 atatccgtct gaaggcgttt cagacg
//
LOCUS       DAWHXS010000156         2791 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWHXS010000156 DAWHXS010000000
VERSION     DAWHXS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.156"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            562..2727
                     /locus_tag="WIE22_001959"
     CDS             562..2727
                     /locus_tag="WIE22_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ4127977.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFEISDKKKGADGKEVD
                     VDDAQKEKNRANEKIVHAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNH
                     GYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKAVGSILVAGQKDRSGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          754 a          799 c          648 g          590 t
ORIGIN      
        1 agtctgttcg gtttcggttt tttccgatag attcctgccc gttaggggtt ctagattccc
       61 gctttcgcgg gaaggacgcg gaaaggttgc ttttcgtcat tcccgcgcag gcctcgtcat
      121 tcccgcgcag gcgggaatct aggttcgttc ggtttcggtt ttttccgata gattcctgcc
      181 gcgttagggg ttctagattc ccgctttcgc gggaaggacg cggaaaggtt gcttttcgtc
      241 attcccgcat ttgttaatcc actatatttc cgccgttttt tatagtggac taacaaaaac
      301 cagtacagcg ttgcctcgcc ttgccgtact atctgtactg tctgcggctt cgtcgcctta
      361 tcctgatttt tgttagtcca ctatacattt ccgacaaaac ctgtcaacaa aaaacaacac
      421 ttcgcaaata aaaacgataa tcagctttac acaaatcccc cctctcgtta atataaacaa
      481 aaataattat tatttttctt atcctgccaa accttaacgg tttggattta cttcccttca
      541 tacactcaaa aggacgaaca aatgaacgcc ccgtttttcc gcctcagcct gctctcgctt
      601 accctggcgg caggttttgc ccatgcggca gaaaataatg ccaatgtcgc attggatacc
      661 gttaccgtaa aaggcgaccg ccaaggcagc aaaatccgta ccaacatcgt tacgcttcaa
      721 caaaaagacg aaagcaccgc aaccgatatg cgcgaactct taaaagaaga gccgtccatc
      781 gatttcggcg gcggcaacgg cacgtcccaa ttcctgacgc tgcgcggtat gggtcagaac
      841 tctgtcgaca tcaaggtgga caacgcctat tccgacagcc aaatccttta ccaccaaggc
      901 agatttattg tcgatcccgc tttggttaaa gtcgtttccg tacaaaaagg cgcgggttcc
      961 gcctctgccg gtatcggcgc gaccaacggc gcgattatcg ccaaaaccgt cgatgcccaa
     1021 gacctgctca aaggcttgga taaaaactgg ggcgtgcgcc tcaacagcgg ctttgccagc
     1081 aacgaaggcg taagctacgg cgccagcgta ttcggaaaag agggcaactt cgacggtttg
     1141 ttctcttaca accgcaacga tgaaaaagat tacgaagccg gcaaaggttt ccgcaatgtc
     1201 aacggcggca aaaccgtacc gtacagcgcg ctggacaaac gcagctacct cgccaaaatc
     1261 ggaacaactt tcggcgacga cgaccaccgc atcgtgttga gccacatgaa agaccaacac
     1321 cggggcatcc gcactgtgcg tgaagaattt accgtcggcg acaaaagttc acggataaat
     1381 attgaccgcc aagcccctgc ttaccgcgaa actacccaat ccaacaccaa cttggcgtac
     1441 acgggtaaaa acctgggctt tgtcgaaaaa ctggatgcca acgcctatgt gttggaaaaa
     1501 gaacgctatt ccgccgatga cagcggcacc ggctacgcag gcaatgtaaa aggccccaac
     1561 catacccgaa tcaccactcg tggtgcgaac ttcaacttcg acagccgcct tgccgaacaa
     1621 accctgctga aatacggtat caactaccgc catcaggaaa tcaaaccgca agcgtttttg
     1681 aactcgaaat ttgagatctc cgataagaaa aaaggagccg acggaaaaga agtcgacgta
     1741 gatgatgcac aaaaagaaaa aaatcgtgcc aatgaaaaaa ttgttcacgc ctacaaactg
     1801 tctaacccga ccaaaaccga taccggcgcg tatatcgaag ccattcacga gattgacggc
     1861 tttaccctga ccggcgggct gcgttacgac cgcttcaagg tgaaaaccca tgacggcaaa
     1921 accgtttcaa gcagcaacct taacccgagt ttcggtgtga tttggcagcc gcacgaacac
     1981 tggagcttca gcgcgagcca caactacgcc agccgcagcc cgcgcctgta tgacgcgctg
     2041 caaacccacg gtaaacgcgg catcatctcg attgccgacg gcacgaaagc cgaacgcgcg
     2101 cgcaataccg aaatcggctt caactacaac gacggcacgt ttgccgccaa cggcagctac
     2161 ttctggcaaa ccatcaaaga cgcgcttgcc aatccgcaaa accgccacgt ctctgccgct
     2221 gtccgtgaag ccgtcaatgc cggttacatc aaaaaccacg gttacgaatt gggcgcgtcc
     2281 taccgcaccg gcggcctgac tgccaaagtc ggcgtaagcc acagcaaacc gcgcttttac
     2341 gatacgcaca aagacaagct gttgagcgcg aatcctgaat ttggcgcaca agtcggccgc
     2401 acttggacag cctcccttgc ctaccgcttc aaaaacccga atctggaaat cggctggcgc
     2461 ggacgctatg ttcaaaaagc cgtgggttcg atattggtgg caggtcaaaa agaccgcagc
     2521 ggcaaattgg aaaacgttgt acgccaaggt ttcggtgtga acgatgtctt cgccaactgg
     2581 aaaccgctgg gcaaagacac gctcaatgtt aatctttcgg ttaacaacgt gttcaacacg
     2641 ttctactatc cgcacagcca acgctggacc aataccctgc cgggcgtggg ccgcgatgta
     2701 cgcttgggcg tgaactacaa gttctaaaac gcacatcccg aaaaaatgcc gtctgaaagc
     2761 ctttcagacg gcatctgttc tgataatttg a
//
LOCUS       DAWHXS010000157         2745 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWHXS010000157 DAWHXS010000000
VERSION     DAWHXS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.157"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            84..203
                     /locus_tag="WIE22_001960"
     CDS             84..203
                     /locus_tag="WIE22_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ4127978.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            214..1184
                     /locus_tag="WIE22_001961"
                     /pseudo
     CDS             214..1184
                     /locus_tag="WIE22_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(1235..2287)
                     /gene="sohB"
                     /locus_tag="WIE22_001962"
     CDS             complement(1235..2287)
                     /gene="sohB"
                     /locus_tag="WIE22_001962"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ4127979.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2391..2480)
                     /locus_tag="WIE22_001963"
     tRNA            complement(2391..2480)
                     /locus_tag="WIE22_001963"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2444..2446),aa:Leu,seq:taa)
     gene            complement(2583..2656)
                     /locus_tag="WIE22_001964"
     tRNA            complement(2583..2656)
                     /locus_tag="WIE22_001964"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2622..2624),aa:Cys,seq:gca)
BASE COUNT          646 a          720 c          656 g          723 t
ORIGIN      
        1 tttgggtaga attaggagta attaggagcg aaaatagacg aaaacctgtg tttgggtttc
       61 ggctgtcggg agggaaagga attttgcaaa ggtctcacaa agtatttgcc acattcggcg
      121 acaagcgcac aacccggctg tttgccgatt tggatgcact ggctgaagtg atggaaaaaa
      181 caatctcgga aaataaaaaa taggggggca aatatgtgga aaatgttgaa acacatagcc
      241 caaacccacc gcaagcgatt gattggcaca ttttccctgg tcggactgga aaaccttttg
      301 atgctggtgt atccggtgtt tggcggccgg gcgatcaatg ccgtgattgc gggggaggtg
      361 tggcaggcgt tgctgtacgc tttggttgtg cttttaatgt ggctggtcgg tgcggcgcgg
      421 cggattgccg atacacgcac gtttacgcgg atttataccg aaatcgccgt gccggttgtg
      481 ttggaacagc ggcagcggta agtcccgcat tcggcaatat agtggattaa atttaaacca
      541 gtacggcgtt gtctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
      601 ctgatttttg ttaatccact ataactgcgc gggttgccct gtcgcgtgag tttgtcagct
      661 tttttgaaga acacctgccg attgccgcga catccgtcgt atccatattc ggcgcgtgca
      721 tcatgctgct ggtgctggaa ttttgggtcg gcgtgtcggc ggtgggcata cttgcgttgt
      781 ttttatggct tttgccacgt tttgcggcca tcagcgaaaa cctgtatttc cgcctgaaga
      841 acagcttgga acgcgacaac cactttatcc gaaaaggcga ccggcggcag ctgtaccgcc
      901 attacggact gcttgcgcgc ctgcgtgtgc tgatttccaa ccgcgaagcc ttcggctatc
      961 tctgcgtcgg cacggcgatg ggtattttgt tcggctttgc ttttgtgatg atgacgctca
     1021 aaggctacag cagcgcgggg catgtctatt cggtcggcac ttatctgtgg atgtttgcca
     1081 tgagtttgga cgacgtgccg cgattggtcg aacaatattc caatttgaaa gacatcggac
     1141 aacggataga gtggtcggaa cggaacatca aagccggaac ttgaaaaatg ccgtctgaac
     1201 acgcttcaga cggcatttcc atccgttcgg caaactacat cacatccgcc cgccggttga
     1261 caagtttggc aaacaacttt tcaacagaag cttccgcctg caaaccaatg cgctggatca
     1321 ggctttgctt ctcctgatat ttcacttcga taacctgttt gttttcaaac gctttcaaca
     1381 acaaatcatc actggtcgaa atctcgtcaa tcaagttcaa cgccaacgcc tgccgaccga
     1441 accaatgctc gcccgttgcc acttcctcaa tatccaattg agggcggttc tcgctgacaa
     1501 actgcttgaa caactgatgc gtttcctcca gttcctgtcg gaatttctgt ttgccctttt
     1561 ccgtattttc acccataaaa gtaaccgtgc gcttaaattc gcccgccgtc atcacatcca
     1621 catcaatatc atgttttttc aacaggcggt ggatattcgg tacttccgcc accacaccca
     1681 ccgaaccgac aatcgcaaac ggagcggaaa caattttatc cgccacacac gccatcatat
     1741 aaccgccgct cgccgccacc ttatcgacgg cgacggtcag cggaatattg cgttcgcgca
     1801 aacgcctaag ctgtgaagcc gccaaaccgt aaccgtgaac cacgccgccc ggactttcca
     1861 atctaagcag aacctcatct tcaggcttgg caatcaaaag caccgccgta atctcatgac
     1921 gcaaggattc tacggcgtgt gcatacaaat cgccgtcaaa atccaacaca aaaaggcggg
     1981 atttttgcgt ttcggcagat ttctccccac cctccttcaa acgctttttc tctgctttgg
     2041 cttccgcctt ttcctttttc ttttcctctt tttcctgatg ttttgcctct tccccgctta
     2101 aaaagaatgc ttcaaacgat tgccgctgtt ttttataatt ttccgaaaaa tccgtcagta
     2161 cgacactgcc gctttccgac tgtttcttac tctgtacgat agccaacaca atcagcgcaa
     2221 ttgcgccgaa cacggtaagc agttcgagca ggaaaatacc gtaattcagt aaaatttctt
     2281 tccacattga ttggatttcc tcttgttcag gcatgaacat gtcaatattg tccatcaccg
     2341 tccgacagat aaaaaaataa ccgcttggag cggcattgtc attttcagct tggtgcccgg
     2401 agccggaatc gaaccggcac gggatgttta gtcccgacgg attttaagtc cgttgtgtct
     2461 acctatttca ccacccgggc atttgtgaaa ggtggaggcg ggggcgcgga ttttaaccgg
     2521 cctatatgaa gattgcactc ctcatagcat aaacactctg ccaccccgcc atagtacgat
     2581 aatggaggcg agagtcggaa tcgaaccggc gtagacggat ttgcaatccg ctgcataacc
     2641 actttgctat ctcgccctaa aactggctta tctaaaaaac ttggagcggg aaacgagtct
     2701 cgaactcgcg acctcaacct tggcaaggtt gcgctctacc aactg
//
LOCUS       DAWHXS010000158         2609 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWHXS010000158 DAWHXS010000000
VERSION     DAWHXS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.158"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            108..671
                     /gene="gmhB"
                     /locus_tag="WIE22_001965"
     CDS             108..671
                     /gene="gmhB"
                     /locus_tag="WIE22_001965"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ4127980.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            700..1443
                     /locus_tag="WIE22_001966"
     CDS             700..1443
                     /locus_tag="WIE22_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ4127981.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPYASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1440..2132
                     /locus_tag="WIE22_001967"
     CDS             1440..2132
                     /locus_tag="WIE22_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ4127982.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHSRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
BASE COUNT          623 a          864 c          627 g          495 t
ORIGIN      
        1 accgtactgg tttttgttaa tccactatat cacgcggcac aggcgcatca ctgccgatat
       61 ttcagtataa tgccaccccc gacctgcccc aacccaaagg aaacgcgatg aaactcatca
      121 ttctcgaccg cgacggcgtc atcaatcagg accgcgatga cttcgtcaaa tccgttgacg
      181 agtggatacc tgtcgaaggc agcatggatg cggtggcatt tctgacgcag gcaggctgca
      241 ccgtcgccgt tgccaccaac caatccggca tcgggcgcaa atattttacc gttcaaaacc
      301 ttaccgaaat gcacgccaaa atgcaccgcc tcgtccgtca ggcaggcggc gaaatcaacg
      361 gcatctggtt ctgcccgcac accgatgccg acaactgcaa ctgccgcaag cccaaaccgg
      421 gtatgattga agacatcatc ggacgcttca acgcccaagc ctcggaaacc tggctggtcg
      481 gcgacagcct gcgcgatttg caggcaatcg atgccgtcgg cggcaaaccc gcgctggttc
      541 tgaccggaaa aggcaaaaaa acgctctccc aacacggaca cgaattgccc gaacacacac
      601 aggttttcga taccctgctc gatttctcac aatacatcat gcaggaaaac accgcaccgc
      661 aagccgactg aacataccgc attccgacaa ggcaaaacca tgctcatcat ccgcaacctg
      721 atttactggc tgatactctg ttccaccctg attttcctct ttccctttat gctgctcgcc
      781 tcgcctttcc gagacggggc gcacaagatg gcgcgggtct gggtcaaaat cctcaacctc
      841 tcgctcaaac acatcgtcgg gctcaaatac cgcatcatcg gcgcggaaaa catccccgac
      901 cgccccgccg tcatctgcgc caaacaccaa agcggctggg aaacgctcgc ccttcaggac
      961 atttttccgc cgcaggttta cgttgccaaa cgcgagttgt tcaaaatccc ctttttcggc
     1021 tggggcttga aactggtcaa aaccataggc atagaccgca acaaccgccg cgaagccaac
     1081 gagcagctca taaaacaggg gttggtgcgc aaaaacgaag gctattggat taccattttc
     1141 cccgaaggca cgcgccttgc gcccggaaaa cgcggcaaat acaaactcgg cggcgcgcgc
     1201 atggcgaaaa tgtttgagat ggacatcgtc cccgtcgccc tcaacagcgg cgaattttgg
     1261 ccgaaaaact cctttctgaa atatccgggg gaaatcaccg tcgtcatctg tccgaccatc
     1321 ccgtacgcaa gcggcagcga agccgaattg atgggaaaat gcgaacacct catcgaaacg
     1381 cagcagccgc tcatttccgg cgcaggcccg tttgccgcca aaatgccgtc tgaaaccgca
     1441 tgaccgcctt tgtccacacc ctttcagacg gcatggaact gaccgtcgaa atcaagcgcc
     1501 gtgccaagaa aaacctgatt atccgccccg ccggcacaca taccgtccgc atcagcgtcc
     1561 caccctgctt ctccgtctcc gctctaaacc gctggctgta tgaaaacgaa gccgtcctgc
     1621 ggcaaacact ggcgaaaaca ccgccgccgc aaactgccga aaaccggctg cccgaatcca
     1681 tcctcttcca cagcagacag cttgccctca ccgcccatca agacacgcaa atcctgctga
     1741 tgccgtctga aatccgtgtt cccgaaggcg cacccgaaaa acagcttgcg ctgctgcggg
     1801 actttttgga acggcaggcg cacagttacc tgattccccg cctcgaacgc cacgcccgca
     1861 ccacacaact gttccccgcc tcctcctcgc tgacctctgc caaaaccttc tggggcgtgt
     1921 gccgcaaaac cacaggcata cgcctcaact ggcggctggt cggcgcaccg gaatacgttg
     1981 ccgactatgt ctgcatacac gaactctgcc acctcgccca tcccgaccac agccccgcct
     2041 tttgggaact gacccgccgc ttcgccccct acacgcccaa agcgaaacag tggctcaaaa
     2101 tccacggcag ggaacttttc gccttaggct gacgcggacc ggaccgaccc gccgctttca
     2161 gacggcatcc gtgccggaac aggcacgcgc ccgcccgatt caaaccgcga tgacgctttg
     2221 ccgccggttc ggggcaggat ggcggcacac acgccgtctg ccgcgtttca tttcacacca
     2281 ctcttccgaa acccgaaacc cgcccggtcc gacgtgcggt atgaaacgct taagctgacg
     2341 cgaagtcttt tactgatttg cccgcgaaaa tgccgtctga aaggttttcg gacggcattt
     2401 ttttgcgttt cctaggatgg cggcggattc gtaaaaggcg gtcagggtgg attgtaggat
     2461 gggttgagac ctgccgaatc cgccgcatct gccaaatcta ccgccgtcat tcctacgaaa
     2521 gtgggaatct agaacgcggg gttaagaaaa cctgcatccc gtcattccca cgaaagtggg
     2581 aatctagttt tgtcggtgcg gaaacttat
//
LOCUS       DAWHXS010000159         2507 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWHXS010000159 DAWHXS010000000
VERSION     DAWHXS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.159"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            170..793
                     /gene="lolA"
                     /locus_tag="WIE22_001968"
     CDS             170..793
                     /gene="lolA"
                     /locus_tag="WIE22_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ4127983.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1066..2205
                     /locus_tag="WIE22_001969"
     CDS             1066..2205
                     /locus_tag="WIE22_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ4127984.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAHPPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2340..>2507
                     /locus_tag="WIE22_001970"
     CDS             2340..>2507
                     /locus_tag="WIE22_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4127985.1"
                     /translation="MNLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT          679 a          734 c          619 g          475 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg cctgcctttt ccgagcggtc aaaaaaatca
       61 gccctcggaa aacgctgttt gtaaaatgca aaccgcccgt aacgccgccc gtatgattgt
      121 tttgccgcgc cgatacttta cgccacaccc atcccaacaa ggaaaaataa tgatgaaacc
      181 gcacaacctg ttccaatttc tcgccgtttg ctccctgacc gtctccgtcg cttccgcaca
      241 ggcgggcgcg gtggacgcgc tcaagcaatt caacaacgat gccgacggta tcagcggcag
      301 cttcacccaa accgtccaaa gcaaaaagaa aacccaaacc gcgcacggca cgttcaaaat
      361 cctgcgcccg ggcctcttca aatgggaata cacttcgccc tacaaacaga ctattgtcgg
      421 cgacggtcaa accgtttggc tctacgatgt tgatttggca caagtgacca agtcgtccca
      481 agaccaggcc ataggcggca gccccgccgc catcctgtcg aacaaaaccg ccctcgaaag
      541 cagctacacg ctgaaagagg acggttcgtc caacggcatc gattatgtgc tggcaacgcc
      601 caaacgcaac aatgccggct accaatacat ccgcatcggc ttcaaaggcg gcaacctcgc
      661 cgccatgcag ctgaaagaca gcttcggcaa ccaaacctcc atcagtttcg gcggtttgaa
      721 taccaatccc caactctcgc gcggcgcgtt caagtttacc ccgcccaaag gcgtggacgt
      781 gttgagcaac tgatgccgtc cgccccgatg ccgtctgaaa gccgccgagg cttcagacgg
      841 cattttcacg caggcggaac aatgtcccgc attaccgccc gatcgggcat cggaacggca
      901 aacccgtgaa aattaacggt tgcgccccgg ttgtttttgc cgtttaatgc aaaccttgct
      961 gcaccaaggg ccaagaaagc cgaccggccg cccccacagc ttccgatgca ggcggcccgg
     1021 ccgtccctgc aatgtttttt atttttgaac gaaaggtcga aaaccatgaa aaaaacactg
     1081 gtggcggcgg caatcctgag cctcgccttg actgcgtgcg gcggcggaag cgataccgcc
     1141 gcccaccccc cctccgccaa gcccgaagcc gaacaatcgg gcaaactcaa tatctacaac
     1201 tggtcggatt atgtcgatcc cgaaaccgtc gccgcctttg aaaaagaaac cggcatcaag
     1261 acgcgttccg attattacga cagcaacgaa acactggagg caaaagtcct gaccggcaaa
     1321 tccggctacg acctgaccgc gccgtccatc gccaacgtcg gccggcaaat caaagcgggc
     1381 gcgtatcaga aaatcgacaa ggcgcaaatc ccccattacg gcaacatcga taaagatttg
     1441 ctgaaaatga tggaagccgt cgatccgggc aacgaatacg ccgtccccta tttctggggc
     1501 atcaacacct tggcaatcaa cacgcggcag gtgcaaaagg cattgggtac ggacaagctg
     1561 cccgaaaacg aatgggattt ggtgttcaac cctgaataca ccgccaaact caaatcctgc
     1621 ggcatcagct atttcgacag cgcaatcgaa cagattcctt tggcgttgca ctatttgggc
     1681 aaagacccca acagtgaaaa tcccgaagac atcaaagccg ccgtcgatat gatgaaagcc
     1741 gtccgaggcg acgtgaaacg cttcagctct tccggctata tcgacgatat ggcggcgggc
     1801 aacctgtgtg ccgccatcgg ttacggcggc gatttgaaca ttgccaaaac ccgtgccgaa
     1861 gaagccgcaa acggcgtgga aatcaaagta ttgaccccga aaaccggcgt gggcgtgtgg
     1921 gtggattcct ttatgattcc gcgcgacgcg caaaacgtcg ccaacgccca ccgctatatc
     1981 gactacacgc tccggcccga ggtggcggcg aaaaacggca gcttcgttac ctacgcgccc
     2041 gccagccgtc ccgcgcgcga gctgatggat gaaaaataca cctccgacgc atcgattttc
     2101 ccgaacaaag aactgatgga aaaaagtttc atcgtatcgc ccaaatccgc agaatccgcc
     2161 aaactgggcg tgaagctgtg gcaagggctc aaggccggaa aataaccgca acccctgccg
     2221 tctgaaacct ttcgggcggc aggaaacggc gcgtccgtta tcaaacaggg gggggcgttt
     2281 cccctcctgc cggttatgat tgggttaaga ttaaaatgat ttagtaaaat gagaaagata
     2341 tgaatttaag tatcgtagtt cccatttata atgtcgaaag ttatttggaa gcgtgtttaa
     2401 gttccataga acctatatta agtaatgaaa atgtcgaact tatccttgtg aatgacgggt
     2461 caaaagacgg aagtgaagat atatgttata aatatataga taaaata
//
LOCUS       DAWHXS010000160         2488 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWHXS010000160 DAWHXS010000000
VERSION     DAWHXS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.160"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..326
                     /locus_tag="WIE22_001971"
     CDS             <1..326
                     /locus_tag="WIE22_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ4127986.1"
                     /translation="ATTVPAQQTFLDAAELMQWSIETEWLGLNVIAHCNFGKQYAQVE
                     FEACFQDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGKY
                     LKPVA"
     gene            complement(545..1339)
                     /locus_tag="WIE22_001972"
     CDS             complement(545..1339)
                     /locus_tag="WIE22_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ4127987.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1383..1487
                     /locus_tag="WIE22_001973"
                     /pseudo
     CDS             1383..1487
                     /locus_tag="WIE22_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1548..2273
                     /locus_tag="WIE22_001974"
     CDS             1548..2273
                     /locus_tag="WIE22_001974"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ4127988.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          676 a          769 c          557 g          486 t
ORIGIN      
        1 tcgctactac cgttccggcg caacaaacat tcctcgatgc cgccgaactg atgcaatgga
       61 gtatagaaac cgaatggctg ggcttgaatg tcatcgcaca ctgcaacttc ggcaaacagt
      121 acgcccaagt cgaatttgaa gcctgcttcc aagacggaca acaccgatcc gcacatcacg
      181 aactgtccgg cttcgtcaac atcggcggac aatggtattt tatcgatccc accgttccgc
      241 atcctgcgat gaaacaaccc tgcatttgcg gatcgggcaa aaaattcaaa gcctgctgcg
      301 gcaaatatct gaaacctgtc gcataaaaat gccgtctgaa cgttcagacg gcattttcaa
      361 cgtgcaaaaa aaaaccattc ataccaaggg taagtatgaa tggtcaatac attgcgggaa
      421 aacgtcttac ttgctgcact gccgaaaagg gagaaacggc agcggtaatc agcggaaagg
      481 attgtacccg aattaatgtt aagaaacgtt aatcgtgaaa atatattaac aaacctgttg
      541 aaacctattg gttttcccgt atccactcga cccagcgttc aaacagcttc ggttcgagcg
      601 cggcgacgac cgagcgtttg aacacgtgtt caccactcca aaacccgtcg ccttccaaag
      661 tcgtcagcct gccgcccgcc tcctcgaaaa tcaacgcgcc ggcggcataa tcccacagct
      721 tctgcccgcc gtggacataa acatcataac gcccgcacgc cagataacac cagtccaacg
      781 tactgctgcc catactccgt atcgttccaa aaggcgcgag cgtactcatg cggctggaca
      841 gtttgcccga acgcagatat ttgatttcca cgcccgcgat tgcctcattc aacttcttat
      901 ccacgagacg caaaggcagg cgtgtcccgt ttaaaaacgc accctgcccg cgttcagcat
      961 aaaaacattc gccgctgacc ggattgtaga ttacgcccaa ttcggcgcgc ccgttgcgga
     1021 caaacgccac cgacacggca aaatgcggca gcccgttgac aaaattgttt gtcccgtcta
     1081 tcggatcgac aatccacagc cccttttccc ccgaatattg ttcccacaga gccgactgtt
     1141 cctgtcgcga catttcctcg cccaacatcg ggcaatcgat cagaagcggc aacgcggcgg
     1201 caaaagccgt ctgcgcggca atgtccgcct cgctcaacat cgaaccgtct tccttgcggt
     1261 gggacggcgt attcaaaaaa cgcggcataa tttcggtttg cgcgatatgg cgcacgactt
     1321 tctgcaaacg gtgtaacact tcctactgtc ctcatatttt gaacttgcgg cacgcgaacg
     1381 tatatagtgg attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     1441 tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa tccactataa tgtccgtttc
     1501 catcacgccg ctgcgacaga ttataaccgt ccgaaccgcc aaaaactatg ccccgattcc
     1561 acctgcccga aaacctttcc gtcggacaaa ccgtcgccct gcccgacaac atcgtccgcc
     1621 acctcaacgt cctgcgcgtc cgccccaacg aaaacatcac cctcttcgac ggcaaaggca
     1681 aggcacacgc cgcacggctg accgttttgg aaaaacgccg cgccgaagcc gaaatcctgc
     1741 acgaagacac aaccgacaac gagtccccgc tcaacatcac actgatacaa tccatctcct
     1801 ccggcgatcg catggatttc accctgcaaa aaagcgtcga actcggcgta accgccatac
     1861 agcccgtcat cagcgaacgc tgcatcgtcc gcctcgatgg ggaacgcgcc gccaaacgcc
     1921 tcgcacgctg gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc
     1981 cccccgtgct gcccatcatc ggctatcgtg aagcactcga caaaatgccg tctgaaagca
     2041 ccaagctgat tatgagcatc aaccgcgccc gcaaactcgg cgacatccgc cacccgtccg
     2101 gcgcaatcgt ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg
     2161 catttgaagc tggctttcag gcggttacac tcggcaaacg gattttacgc acagaaaccg
     2221 ccccactcgc cgccctcgcc gccatgcaga cgctttgggg cgatttcgca taaacagaaa
     2281 tgccgtctga aacccgttca gacggcattt tgcagccgat taagatagta ggttcaaata
     2341 agatttcccg tgtcgtcatt cccgcgaaag cgggaatcta gaaacgaaaa actacaggga
     2401 tttatccgaa acaacaaacc ctctccgcca tcattcccgc aaaagcggga atctagaaac
     2461 gaaaaactac agggatttat ccgaaaca
//
LOCUS       DAWHXS010000161         2451 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWHXS010000161 DAWHXS010000000
VERSION     DAWHXS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.161"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(228..1862)
                     /gene="groL"
                     /locus_tag="WIE22_001975"
     CDS             complement(228..1862)
                     /gene="groL"
                     /locus_tag="WIE22_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ4127989.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAIESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(1956..2243)
                     /locus_tag="WIE22_001976"
     CDS             complement(1956..2243)
                     /locus_tag="WIE22_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ4127990.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVE"
BASE COUNT          491 a          645 c          643 g          672 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaacaaaa atgcaaagaa
      121 cggcgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaaccgcac
      181 ggcgttgacc gtgcggcttg ttgtctgaaa gcttcagacg gcatttctta catcatgccg
      241 cccataccgc ccatgccgcc catgtcaggc atagcaggtt tttcttcagg gatttcagcg
      301 atcatgcagt ctgtggtcag catcagaccg gcgatagatg cggcgtgttg cagcgcggaa
      361 cgggttactt tggcaggatc gagtacgccc atttcaatca tatcgccgta ttcgccgctg
      421 ccagcgttgt aaccgtagtt gcctttgcct tccaacactt tgttcacgac tacgctgggt
      481 tcgccgcctg cgttggcaac gatttggcgc agcggagact caatggcgcg caagacgatt
      541 tgtacgcctg cgtcttgatc ggcattgccg gtgtgcaggt tttccagagc ggcacgggcg
      601 cgcaacaggg ctacgccgcc gcctgcaacc acgccttctt caacggctgc gcgggtagca
      661 tgcagcgcgt cttctacgcg gtcttttttc tctttcattt cgacttcggt agcggcaccg
      721 actttgatta ctgccacgcc gcctgccagt ttggccacgc gctcttgcag tttttctttg
      781 tcgtaatcgc tggttgcggt ttcgatttgt tggcggattt cggcaacacg cgcttcgatt
      841 tgggctgcgt cgccgaagcc gtcgatgatg gtggtgtttt ctttaccgat ttcgatgcgt
      901 ttggcttgac ccaagtcgtc caaagtcgct ttttccagag acaggccgac ttcttcggaa
      961 atcaccgtgc cgccggtcag gatggcgatg tcttgcagca tcgctttgcg gcggtcgcca
     1021 aagcccggag ctttaacggc aacggttttc aggatgccgc gaatgttgtt taccaccaaa
     1081 gtcgccaagg cttcgccttc tacgtcttca gcgataatca agagcggacg gctggctttt
     1141 gccacttgtt ccaaaacagg caacaagtcg cggatgttgc tgattttttt gtcgaacagc
     1201 aatacaaacg gattgtccaa gccggcgatt tgtttttccg catcgttgat gaagtaagga
     1261 gacaggtagc cgcggtcgaa ttgcatacct tcaaccacgt ccagctcgtt ttccaaagat
     1321 ttgccgtctt caacggtaat cacgccttct ttgccgactt tttccatcgc ttcggcaata
     1381 atcgcgccga cttgttcgtc ggagttggcg gaaatcgagc cgacttgggc gatttcttta
     1441 gaagtgtcgc aaggtttggc gatgtttttc agctcttcaa ccaaagcggc gacggctttg
     1501 tcgataccgc gtttcaggtc ggtcgggttc ataccggcgg taacgtattt cataccttcg
     1561 gcaacgatgg attgcgccag tacggtggcg gtagtcgtac cgtcgcccgc cacgtcgttg
     1621 gttttggacg cgacttcttt taccatttgc gcgcccatat tttcaaactt gtctttcagt
     1681 tcgatttctt tagcgacggt tacgccgtct ttggtaatgt gcgggccgcc gaaagcgcgg
     1741 tcaaccacca cgttgcggcc tttgggaccc agggttacgc gcacggcgtt tgccagaatg
     1801 ttcacgccgt ttaccatttt ttgacggact tcattgccga attgtacgtc ttttgctgcc
     1861 atttcaattc tccaaaaatc attaaaactg tctgataaaa ccgtttatgc cgtctgaagg
     1921 cggtttgccg tttcagacgg catcatcatt cgcacttatt caacgatgcc gaaaatatct
     1981 tcttcgcgca ttaccaacag ctcttcgccg tcggctttta cggtttggcc gctgtatttg
     2041 ccgaaaatga ttttgtcgcc gactttgaca tccagcggac ggcgcgcgcc gtctttaccg
     2101 attttgcccg cgcccacggc gatgacttcg cccatatcgg gtttttcggc ggccgcaccc
     2161 ggcaaaacga tacccgatgc ggttttttct tcagcttcca agcgtttgac gacgacgcgg
     2221 tcgtgtaaag gacggatggt catatttatg ctccgataaa atggtttgaa aacaatcatc
     2281 tgcccgaacg gttcggacag attgaagtgg aaaccgggct gccgcctgcc ggccgcccgt
     2341 atgaatcggc aaaattaggg tgtgtgcggg caaattcaag tgaggcggaa aaaatttatt
     2401 tccgccgttt tttatagtgg actaaattta aggggctgta ctagattagc a
//
LOCUS       DAWHXS010000162         2442 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWHXS010000162 DAWHXS010000000
VERSION     DAWHXS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.162"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            35..1390
                     /locus_tag="WIE22_001977"
     CDS             35..1390
                     /locus_tag="WIE22_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ4127991.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2413
                     /locus_tag="WIE22_001978"
     CDS             1394..2413
                     /locus_tag="WIE22_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ4127992.1"
                     /translation="MSDTPATPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVTYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDGL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRCRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYG
                     IESLS"
BASE COUNT          435 a          672 c          742 g          593 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcaaa tatggcgatg cagccgttta agatgatggt
      481 cggcgacatg gtcaacgagg agcagaaagg ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg tttgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactcttg aaaaccgcgc ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccacc gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccccgct acccgcgatt tcggcctgat
     1441 cgacgggcgt gccgtaaccg gctatgtgct gtccaatcgg cgtggtacgc gtgtctgcgt
     1501 gctggacttg ggcgggattg tgcaggaatt ttccgttttg gcagacggcg tgcgcgaaaa
     1561 cctcgtggtg tcgttcgacg atgcggcttc ctatgcggac aatccgtttc agattaacaa
     1621 gcagataggg cgcgtggccg gacgcatccg cggtgcggcg ttcgacatca acggcaggac
     1681 ttaccgcgtg gaggccaacg aaggcaggaa cgcgctgcac ggcggttcgc acgggctggc
     1741 cgttacccgt ttcaacgcgg tggcggcaga cggccgttcg gtggtgctgc gcagccgcct
     1801 gcaacagtcg gccgacggtt atcccaacga tttggatttg gatatttcct accgcttgga
     1861 cgaggacgac cggcttaccg ttacctatcg cgccaccgcg ctcggcgaca cggtgttcga
     1921 cccgacgctg cacatttact ggcggctgga cgcgggcctg cacgatgcgg ttctgcatat
     1981 tccgcagggc ggacatattc cggccgatgc cgaaaaactg cccgtctcaa cggtttcaga
     2041 cggcctcgaa gtatttgatt tcagccggcc caagccgctg gatgccgccg ttgccgccct
     2101 gcgccgcgaa acgggtcggg ccggttttga cgacgcttac cgcgtgccgt ccgatatagg
     2161 ccgtcccgcc gctgtgttgc aagccggacg ccgctgtcgt atcagcatat acagcgaccg
     2221 caacggcttg gtcatcttta ccgccgcccc gcaggatttc gcgcggcacg atgcgggcgt
     2281 ttacgacgcg ctggcgaccg aggcgcagac gctgcccgac agcctgcatt ggcccgagtt
     2341 cggcaatatt cgtctgaaca agggtgatac cagggaggcg acgattgctt acggcatcga
     2401 atccctttct taggagcttc ctaacaccgg ttgcagacgg cc
//
LOCUS       DAWHXS010000163         2402 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWHXS010000163 DAWHXS010000000
VERSION     DAWHXS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.163"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            111..668
                     /locus_tag="WIE22_001979"
     CDS             111..668
                     /locus_tag="WIE22_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ4127993.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAIADITRTYSAHY
                     LNPNNRNMSLFPQALPCLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICGKLGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQ
                     LLGAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(755..1723)
                     /gene="ispH"
                     /locus_tag="WIE22_001980"
                     /gene_synonym="lytB"
     CDS             complement(755..1723)
                     /gene="ispH"
                     /locus_tag="WIE22_001980"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ4127994.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1752..2249)
                     /locus_tag="WIE22_001981"
     CDS             complement(1752..2249)
                     /locus_tag="WIE22_001981"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ4127995.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVTVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          551 a          708 c          605 g          538 t
ORIGIN      
        1 gcagcccgga tgcctgatta tcaggtatcc gggcaggatt aagggggtat ttgggtagaa
       61 ttaggagata tttggggcga aaacagctga aaacagctga aaacctgtgt ttgggtttcg
      121 gctgtcggga gggaaaggaa ttttgcaaag gtctcagcct gcccgaaatc atccgcgacc
      181 tgctcgaaat gccgtctgaa accgccattg ccgacatcac acgcacttat tccgcacatt
      241 acctcaatcc caacaaccgc aatatgtcct tatttcctca ggcactgccc tgtctggaca
      301 agctcaaagc acaaggatac tggcttgccg tcgccacggg caaagggcgg gcgggtttgg
      361 acaacgccat cagccaaacc gccaccggcg gctattggct cgccaccgcc tgcgcggggg
      421 aatatccctc caaaccctcg cccgaaatgg tattcggaat ctgcggcaaa ctgggacttg
      481 acccgaaaga ggcattggtc gtcggcgata cggcgcacga cctgcatatg gcggcgaacg
      541 cgggtgcggc ggcagtcggc gttgccaccg gcgcacattc gcgcgaacag ctccttggcg
      601 caccgcatct cgccgtattg gacgacttgt ccgaactgcc cggttttctt gcacagcatt
      661 acgcctgatt ggtttccgca tccggcacgc ggcaaaaatg ccgtctgaag cctgttcaga
      721 cggcatttgt tttgcccaaa cattcaacgc ttgctcaacg cttgcacaaa tcgggtttgg
      781 tttcgccctc gcggcgcaac tctttgggca ggacgaacac gatgctttct tccgcaccct
      841 cgccttcgcg cacggtttca tgcccccacc cgcgtatggt tgccaatact tcctgcacca
      901 acacttcggg cgcggacgcg cctgccgtta cgccgacttt gtttttgccc tcaaaccatg
      961 cgcgttgcag gtagcctgca ttatccacca tatacgcatc gattccgcgc gatgccgcca
     1021 cttcgcgcaa gcggttgctg ttagacgaat tgggcgaacc gaccacaatc acgatgtcgc
     1081 actgttctgc caactctttg acggcggttt gccggttggt cgtcgcatag cagatgtctt
     1141 ccttgtgcgg attgcggata ttggggaaac gcgcgttcag cgcggcgatg atgtctttgg
     1201 tttcatcgac cgagagcgtg gtttggctga cataggcgag tttgtcgggg tttctgactt
     1261 cgagttttgc cacatctcct accgtttcga ccaaaagcat tttgcccggc gcaagctgcc
     1321 ccatcgttcc ttcgacctcg acatgaccct tatgcccgat catgatgatt tcacagtctt
     1381 gggcatccag tcgggcgact tccttatgca ctttcgtcac cagcgggcaa gtcgcatcaa
     1441 atacccggaa accacgttcc gccgcttctt gctgcaccgc cttcgatacg ccgtgtgccg
     1501 aataaaccag cgtcgcgccc ggcggcactt ccgccaagtc ttcgataaac accgcgcctt
     1561 tttcacgcag gttatcgacg acgaatttgt tgtggacgac ttcgtggcgc acataaatcg
     1621 gcgcgccgaa ttcttccaaa gcacgttcga caatactgat tgcccgatcc acaccagcgc
     1681 agaagccgcg cggattggca aggatgatgg ttttctcgtt cataagcccg gtatttcgtt
     1741 ttcagacggc atcaatattt ttcttcttgg gttttacggt ggacgatgtt gtccaacact
     1801 gccaacaccg caccgacgca gataaagctg tcggcaatat taaaggcggg ataaaaccaa
     1861 ttttgccaat aaaacaataa gaaatcgacg acatgaccgt gtatcaggcg gtcgatgaca
     1921 ttgcctaacg caccgccgat aatcattgcc gcacccgttt tgccgagggt tgcaaactca
     1981 tcgcgcaaga tggcgcgtac caaatacgcg ctcaccgtca ccgccagcac caaaaaaaag
     2041 tatttttgcc agccgccctg atcggcaagg aagctgaacg ccgcacccgg gttgtacacc
     2101 agcgtcaaat cgaaaaagga aggaatgaca ttgacgcgtt cccgatactg aaacgacgac
     2161 agcaccgccc acttcgacca ctggtccagc acgatggcgg caagtgccaa tacccaatag
     2221 cgcgttttac ttgaaacaga tgaagacata tttttcaaca gtcggataaa agagtgctat
     2281 tttacccgaa aaccccctgc ctgtacccga aacggcaaat gccgtaatct tgaaacccgt
     2341 cattcccgcg caggcgggaa tctagacctg tccgcacaga aacttatcgg ataaaaacag
     2401 tt
//
LOCUS       DAWHXS010000164         2324 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWHXS010000164 DAWHXS010000000
VERSION     DAWHXS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.164"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..705
                     /locus_tag="WIE22_001982"
     CDS             <1..705
                     /locus_tag="WIE22_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ4127996.1"
                     /translation="VEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIP
                     RPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTHPNPEPDPDLNPDANPDTDG
                     QPGTRPDSPAVPDRPNGRHRKERKEGEDGGLLCKFFPDILACDRLPEPNPAEDLNLPS
                     ETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFSFENACTIAERLRYMLLALA
                     WAVAAFFCIRTVSREV"
     gene            1008..1292
                     /locus_tag="WIE22_001983"
     CDS             1008..1292
                     /locus_tag="WIE22_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ4127997.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     HSITDAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQITKLATSIGNKK"
     gene            1293..>2324
                     /locus_tag="WIE22_001984"
     CDS             1293..>2324
                     /locus_tag="WIE22_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ4127998.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFILTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIILLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCVEGGRTGCTCYSHQGTALKEVTQVMCKDYVKNGLP
                     FNPYKEESQG"
BASE COUNT          687 a          565 c          564 g          508 t
ORIGIN      
        1 gtagaagtcg cacccggaac aaaagtgaat atggggcccg tcacggacag gaacgggaat
       61 cccgttcagg ttgtcgcaac attcggcagg gattcgcaag gcaacaccac agtggatgtt
      121 caagtaatcc cgcgtcccga tttaacccct ggaagtgcgg aagcaccgaa cgcacagccg
      181 ctgcccgaag tatcgcccgc cgaaaacccc gcaaacaacc cgaaccccaa tgagaacccc
      241 ggcacgcatc ccaatcccga acccgacccc gatttgaatc ccgatgcaaa tcccgatacg
      301 gacggacagc ccggcacaag acccgattcc cccgccgttc cggatcgccc aaacggtagg
      361 catcgcaaag aaaggaaaga aggcgaagac ggcggccttt tgtgtaaatt cttccccgac
      421 attctcgctt gcgacaggct gcccgagccc aatccggcag aagatttaaa tctgccgtct
      481 gaaaccgtca atgtagagtt tcagaaatcc ggaatcttcc aagattccgc acagtgtccc
      541 gcacctgtca ctttcacagt gactgtgctt gattcaagca ggcagttcgc gttcagcttt
      601 gagaacgcat gtaccatagc cgaacggcta aggtacatgc ttctcgccct tgcttgggcg
      661 gttgccgcct ttttttgtat ccgcacagta tcccgtgaag tctagcaggc gcagcaccgc
      721 cgggcttcag taacttgtgc caaggcaggg ggaggacgtc cagaaagatt tgtaaagacg
      781 gctttatcgt ctttataaat ctttttggat accccttgcc gccccgccaa aagaacacac
      841 tctgccgcaa gggcaggtgg taaggcgcgc gccttttgcg ccgtccccct gcccccgcag
      901 cgtcgcaagt gagactgggg gtgtgggggc tagtccccgc aaaatctttc agattaagaa
      961 acattttttt aatgaggcaa ccgtgccttt taagaaaggg agagcaaatg aaattgttgg
     1021 ccgcattgat tccgctcttg atgagcgtcg caggccgtat attgactgca ttgggattga
     1081 tggccgtgac ctattcgggg gtggatagat tggtagccca ttttcaacag gcgataaccc
     1141 atagcataac ggacgcacct caagcaatgt tacagctttt ctatataagc ggcggtggta
     1201 ctgttctaaa cattcttttc ggcgcgatcg cctttattct gtcattcaaa caaataacaa
     1261 aactagcaac ctcaatcggg aataaaaaat aaatggcaga gatctgtttg ataaccggca
     1321 cacccggttc agggaaaaca ttaaaaatgg tttccatgat ggcaaacgat gaaatgttta
     1381 agccggatga aaacggcata cgccgtaaag tatttacgaa catcaaaggc ttgaagatac
     1441 cgcacaccta catagaaacg gacgcgaaaa agctgccgaa atcgacagat gagcagcttt
     1501 cggcgcatga tatgtacgaa tggataaaga agcccgaaaa tatcgggtct attgtcattg
     1561 tagatgaagc tcaagacgta tggccggcac gctcggcagg ttcaaaaatc cctgaaaatg
     1621 tccaatggct gaatacgcac agacatcagg gcattgatat atttattttg actcaaggcc
     1681 ctaagcttct agatcaaaat cttagaacgc ttgtacggaa acattaccac atcgcttcaa
     1741 acaagatggg tatgcgtacg cttttagaat ggaaaatatg cgcggacgat cccgtaaaaa
     1801 tggcatcaag cgcattctcc agcatttata cactggataa aaaagtctat gacttgtacg
     1861 aatcagcgga agttcatacc gtaaataagg tcaagcggtc aaaatggttt tatactctgc
     1921 cagtaataat attgctgatt cccgtttttg tcggcctgtc ctataaaatg ttaagtagtt
     1981 atggaaaaaa acaggaagaa cccgcagcac aagaatcggc ggcaacagaa cagcaggcag
     2041 tacttccaga taaaacagaa ggagaatcgg tgaataacgg aaaccttacg gcagatatgt
     2101 ttgttccgac attgtccgaa aaacccgaaa gcaagccgat ttataacggt gtaaggcagg
     2161 taagaacctt tgaatatata gcaggctgtg tagaaggcgg aagaaccgga tgcacatgct
     2221 attcgcatca aggtacggca ttgaaagaag tgacgcaagt gatgtgtaag gactatgtaa
     2281 aaaacggctt gccgtttaac ccatacaaag aagaaagcca aggg
//
LOCUS       DAWHXS010000165         2317 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWHXS010000165 DAWHXS010000000
VERSION     DAWHXS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.165"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WIE22_001985"
     CDS             complement(53..1462)
                     /gene="dacB"
                     /locus_tag="WIE22_001985"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ4127999.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1627..2193
                     /locus_tag="WIE22_001986"
     CDS             1627..2193
                     /locus_tag="WIE22_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4128000.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          526 a          563 c          670 g          558 t
ORIGIN      
        1 tcctagttat ctaggacagc cccttaaatt taatccacta tattttcctg tttcaggctc
       61 ggcgttcttt gcacatcagt ttcgcatcca gccagccatc gccgccggag atgatgttgt
      121 tggcaacgaa gttgtccaag tcgggcagca gggaaacggc gcggccgctg ttgatgatga
      181 cgaccaccgc catcggtttg tcgcccagcc aataacctgc aagggcgcgg acattgttga
      241 gcgtgccggt ttttaagcgc aacagcccgc cgctttgttt gaagcggttg cgtaaagtcc
      301 cgtctgtgcc ggcgatgggt agcgtatcga tgaaatcttg tgcaaacggg ctgaaataag
      361 ccgtttccaa catttgcgcc atcattctcg ccgttaccct ttcttttctg gacaggcctg
      421 aaccgttttc caaaaccaaa tccgcaatat cgatgcccga cacggcaagt tcgcgccgga
      481 cggcagatgc cgcctgttcg gaaacggcgg gcagtttgcc gtcgccgccg agtttgagga
      541 agacggaacg cgcaattaga ttgtccgaac gcttgttcat gtccgtcaaa atttccttca
      601 tcggctttga gtgtgcaacg gcaagcgtct gcgcgccttc cggcgtgtcg gatatgccga
      661 tgccgtctga aatccgtccg ccgccgagca gccagtggtt ggtaaaactt tgccggatca
      721 gttcgtcaag cgcgaacatc cggacaccga caggcttgcc caaacagctc tcgggaatat
      781 tgccgcgcaa tttcagcgta ttgtccgaaa aagatgcacg catcagtttt ttgatcgaag
      841 ggcaggcagc ttgggaggcg gtaattttca agttgttttg ggcgaaaata tgcggcaaag
      901 gcggatcggt gaggatgtcg gtactgtcgg cggcattgcg ttcggcgcgc accataacca
      961 taccggcaga cagcatagtt ggattggggg gcgtcataaa cggcgaaccg ctgtcggctt
     1021 cgaaatcgtc ggggctgccg acttcgcccc acaggctgtg gtcgagcatc aggtgtcccg
     1081 tgatattgcg tatgccttgt tcgcgcaact ggcgttggac ggcaagcagg ttttcctgat
     1141 tgaaaacggg gtcgccgctg cccgcccaat acaggtttcc gtcaagcgtg ccgtcgttta
     1201 ccgtaccgtt gcttttaaac tcggtcgccc agcggtaatt gctgccgaag gttttgaagg
     1261 cggcaaacgc ggtaacgagt ttcattgtgg aggcggggtt gacggggaca tccgagcggt
     1321 ggtcaatgat gacttttccg ctgtcaagct cttggacata tacggcgatt tcgttttgcg
     1381 gaatgcggcc ggtatcgagc gcgtgtgcgg cgagggaggc gagaagcagc agcagggagg
     1441 ccgctgtttt ggggaaattc ataggtaaat ctgttacata aaaaaagcaa ttataaggca
     1501 aagccggata agtgggaacg ctgtggcggc gggccggctt gtttgcaggg gaaatcacat
     1561 atataataat cgctatcatt atgaaatgat tgagacgcac aaacttaata ttcaggagga
     1621 atgattatgg ctaagaaaat cagtattttg gtgggcagcc tgcgccgtgc ttcttttgcg
     1681 cgcaaagtgg cattgaatgc ggcggagatg ttccccgaag gctggcaggc ggaaatcgtc
     1741 gaaatcggac atctgccgct ttacaatttc gattatgacg accctgcggt ggaagatgtg
     1801 ccgctgcccg aaagctacac ggctttccgc gaaacgatta aggcttcgga cggcattttg
     1861 ttcgttacgt ccgaaaataa ccgcaccatt cccgcctgtt tgaaaaatgc ggtggacatc
     1921 ggctcgaaac cgaatgccga cgtggcgtgg aaaaacaaac cggccggtat catcagccat
     1981 tccgtcggca agatgggcgg ttacagttcg caaaaaaacc tgcgccttgc gctgtcttat
     2041 ttcgatatgc ccgtaaccgg acagccggaa gtgtttttgg gcaattcgcc gacgctgttt
     2101 gatgaaaacg gcaaattgat cgactcggca agggattttg ttcagtcata tattaatcag
     2161 tttgttggtt tgattgaaag aaatgccaaa taaacaaacc aagtcgaaag gccgcaaaac
     2221 cggttcgggt tttgcggctt ttttatatgg gctgggcagc gtcaaggttt gccgtttgcc
     2281 ggataatgcg cggcaaatga ccggcgtgtt tatagcg
//
LOCUS       DAWHXS010000166         2285 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWHXS010000166 DAWHXS010000000
VERSION     DAWHXS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.166"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="WIE22_001987"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="WIE22_001987"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ4128001.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADIFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WIE22_001988"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WIE22_001988"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ4128002.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="WIE22_001989"
     CDS             complement(1884..2192)
                     /locus_tag="WIE22_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128003.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          462 a          533 c          737 g          553 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcgggccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat atctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat aaggtagggt tcgataacgt cttcgatggt gtctgtagat
      901 tcgccgatgg cggcggcaac attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga gaaatttcct gtccatcacg tccagcccct gcgcgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc gtcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agctgcaaca gttgtgccga acggctgacg
     1201 atggtggtaa ggtctcggtt ttcgtaaaac tcaaggcggg agacgatgcc gaagcggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgatgagcgt gaagggcggc
     1321 aggtcgattt tgacggaacg ggcggcgggt ccttcgccta tcataatgtc gagccggtag
     1381 tcttcgagcg cgggatagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 atgaacaata catcgtgcgg atcaaggttg gtcaaaaggg cggcgaggtc gcctgcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttgcccag tccgggcggg ccgaagagca aaacgtggtc gagtgcttcg
     1621 ccgcgttttt tggcggcttg gatgaaaatg gcaagctgtt ctttggcttt gtcttgcccg
     1681 atgtagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacgat gcgttgcggt tgcgcggcgg tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtcc gaacggacgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttatccg cgggtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgg gggcagatgc cggtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt ggtaggcgtt ggggtcgaag cggatttgct gcatgacggc ttgcgccaat
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa caggcggatt
     2101 tcgccaccgc taaattccgc actgtgtttg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgattttat gtcgggggcg gaatggctga
     2221 agcgtgaatt atagcggata tggcggcggc tgttgcaaag cttggattgg gtatcgtcct
     2281 caaaa
//
LOCUS       DAWHXS010000167         2172 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWHXS010000167 DAWHXS010000000
VERSION     DAWHXS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.167"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WIE22_001990"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WIE22_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ4128004.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQVVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          544 a          622 c          564 g          442 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac ttcggcggcg aagtggagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caataaaatc gacaaaccgt ccgcccgtcc gagctgggtc atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt atgcttccgg
      601 tctgtccggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacgt atcaagcccg gtcaagttgt tgccgtcatg aaccacgatc aacaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggttta gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtattgaa gacatcggca tcggcgtaac
      961 catcaccgac aaagacaacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gaccatggac tttatggtta acaccagccc gttggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagacacc gccgatgccg acgtgttccg cgtatccgga cgcggcgagc tgcacctgac
     1201 cattttgttg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccttat gaaaacctga ctgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgatggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aattcatgac cctgacgcgc ggtgtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcggtcgc ttacgccttg tggaatcttg aagaccgcgg
     1621 ccgtatgttc gtatcaccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 ccgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgcgc
     1741 cagcggcacc gacgaagccg tgcgcctgac cacgccgatc aagctgacgc tggaaggtgc
     1801 ggttgagttt atcgacgatg acgaactcgt tgaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccacttt aaaaagctgg attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggtat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aagcttagga ag
//
LOCUS       DAWHXS010000168         2145 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWHXS010000168 DAWHXS010000000
VERSION     DAWHXS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.168"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            170..1972
                     /locus_tag="WIE22_001991"
     CDS             170..1972
                     /locus_tag="WIE22_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ4128005.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDEEDDEELEPVVRTAPVLSFHSDKEGTGEKEEVGASSNLTVYFDKKGVLK
                     AGTITLKAGDNLKIKQNTNENTNENTNASSFTYSLKKDLTDLTSVGTEKLSFSANGNK
                     VNITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKK
                     RAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGK
                     KTEVKIGAKTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKT
                     TTANGQTGQADKFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQ
                     LQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETININAGNNIEITRNGKNIDIATS
                     MTPQFSSVSLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQL
                     KGVAQNLNNRIDNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIG
                     YSSISDTGNWVIKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          665 a          521 c          530 g          429 t
ORIGIN      
        1 agtttcctta gattccacgt cccagattcc cgccttcgcg ggaatgacga gattttaagt
       61 tgggggaatt tatcagaaaa cccccaaccc caaaaaaccg ggcggatgcc gcaccatccg
      121 cccccaaacc ccgatttaac cattcaaaca aaccaaaaga aaaaacaaaa tgaacaaaat
      181 ataccgcatc atttggaata gtgccctcaa tgcctgggtc gtcgtatccg agctcacacg
      241 caaccacacc aaacgcgcct ccgcaaccgt ggcgaccgcc gtattggcga cactgctgtc
      301 cgcaacggtt caggcgaatg ctaccgatga agaagacgat gaagagttag aacccgtagt
      361 acgcactgct cccgtgttga gcttccattc cgataaagaa ggcacgggag aaaaagaaga
      421 agtaggagca agttcaaatt tgacagtata tttcgacaag aaaggagtac taaaagccgg
      481 aacaatcacc ctcaaagcag gcgacaacct gaaaatcaaa caaaacacca atgaaaacac
      541 caatgaaaac accaatgcca gtagcttcac ctactcgctg aaaaaagacc tcacagatct
      601 gaccagtgtt ggaactgaaa aattatcgtt tagcgcaaac ggcaataaag tcaacatcac
      661 aagcgacacc aaaggcttga attttgcgaa agaaacggct gggacgaacg gcgacaccac
      721 ggttcatctg aacggtattg gttcgacttt gaccgatacg ctgctgaata ccggagcgac
      781 cacaaacgta accaacgaca acgttaccga tgacgagaaa aaacgtgcgg caagcgttaa
      841 agacgtatta aacgcaggct ggaacattaa aggcgttaaa cccggtacaa cagcttccga
      901 taacgttgat ttcgtccgca cttacgacac agtcgagttc ttgagcgcag atacgaaaac
      961 aacgactgtt aatgtggaaa gcaaagacaa cggcaagaaa accgaagtta aaatcggtgc
     1021 gaagacttct gttattaaag aaaaagacgg taagttggtt actggtaaag acaaaggcga
     1081 gaatggttct tctacagacg aaggcgaagg cttagtgact gcaaaagaag tgattgatgc
     1141 agtaaacaag gctggttgga gaatgaaaac aacaaccgct aatggtcaaa caggtcaagc
     1201 tgacaagttt gaaaccgtta catcaggcac aaaagtaacc tttgctagtg gtaatggtac
     1261 aactgcgact gtaagtaaag atgatcaagg caacatcact gttaagtatg atgtaaatgt
     1321 cggcgatgcc ctaaacgtca atcagctgca aaacagcggt tggaatttgg attccaaagc
     1381 ggttgcaggt tcttcgggca aagtcatcag cggcaatgtt tcgccgagca agggaaagat
     1441 ggatgaaacc atcaacatta atgccggcaa caacattgag attacccgca acggtaaaaa
     1501 tatcgacatc gccacttcga tgaccccgca gttttccagc gtttcgctcg gtgcgggggc
     1561 ggatgcgccc actttaagcg tggatgacga gggcgcgttg aatgtcggca gcaaggatgc
     1621 caacaaaccc gtccgcatta ccaatgtcgc cccgggcgtt aaagaggggg atgttacaaa
     1681 cgtcgcacaa cttaaaggtg tggcgcaaaa cttgaacaac cgcatcgaca atgtggacgg
     1741 caacgcgcgt gcgggcatcg cccaagcgat tgcaaccgca ggtttggctc aggcgtattt
     1801 gcccggcaag agtatgatgg cgatcggcgg cggtacttat cgcggcgaag ccggttacgc
     1861 catcggctac tcgagcattt ctgacactgg gaattgggtt atcaagggca cggcttccgg
     1921 caattcgcgc ggtcatttcg gtacttccgc atctgtcggt tatcagtggt aagggctttt
     1981 tcgcctgtct gctgttgggg caggcggaag gtttgaaggg aagggtggcg atttgccgcc
     2041 tgagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatt
     2101 ttccatgagc accttcttcc aacaaaccgc ccaagccatg attgc
//
LOCUS       DAWHXS010000169         2132 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWHXS010000169 DAWHXS010000000
VERSION     DAWHXS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.169"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            59..181
                     /locus_tag="WIE22_001992"
                     /pseudo
     CDS             59..181
                     /locus_tag="WIE22_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            432..1889
                     /locus_tag="WIE22_001993"
     CDS             432..1889
                     /locus_tag="WIE22_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ4128006.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          496 a          614 c          490 g          532 t
ORIGIN      
        1 gtaccggttt ttgttaatcc gctataatgc gcccttcggc gtggcggata tataaggaag
       61 tgattttcca tctaagtata gtggattaaa tttaaatcag gacaaggcga cgaagccgca
      121 gacagtacaa atagtacggc aaggcgaggc aacgccgtac tggtttaaat ttaatccact
      181 ataaaaaacc gccctatcgg ataagccctt aacagaaaag gctttacccg cgccgtatcg
      241 gaacacatcc tctaaaatac aatccgttga attgaaaaaa atataaaaac atccgcccgc
      301 gaaaaacggc agcgcgtcgt ttgacaaaga atgaaaatat cggttaaaaa ccgattttca
      361 tacaaaaaac accgctgccg tccgcatccg tttcagacgg tattgagaga aaatctttta
      421 ggagaacctt tatgtcccgg catcccgccc ccaccggaga aaaaacattc ttcggccacc
      481 ccttccagct ttccaccctc ttccatatcg aattgtggga acgtttttca ttttacggaa
      541 tgcagggcat cctgctgatt tacctctact acaccgccga caaaggcggc ttgggcatag
      601 acaaaaccct cgccggcggc attgtcggcg catacagcgg cagcgtgtac ctgtccacca
      661 ttttgggggc gtggtttgcc gaccgagtat ggggtgcgga aaaaaccctc ttcctctcgg
      721 gcatcgtcgt gatgctcgga cacatcgtcc ttgccgccgc cccgggcctg tacggccttt
      781 taatcgggct gatattcatc gcattgggca gcggcggcgt gaaatctacg gccagttcta
      841 tggtgggcgc attatacgaa caggacgaaa tgcggccgct gcgcgatgcg ggattttcca
      901 ttttctacat cgccatcaat atcggcggct tcctaggccc gctgctgacc ggcctgctgc
      961 aggaaaacat cggtttccat tatggtttcg gcgcggcggc ggtcggtatg gcattcggct
     1021 tgtggcgtta ttccttggga cgtaaaaacc tgccccatcc catcgtcccc catccgcttt
     1081 caaaaggaca gggcaaaact gcggccgccg tcggcattgc tctcatcgcc gcacttgcaa
     1141 ccgccatcaa aaccgggctt gtcaacctcg acaatttctc cggcatcctc ttatctaccg
     1201 tcatccttgc cgtcattgcc tattttgccc gcctgctgac taatccccgc gtcagttccg
     1261 acaacaaacg gcacatcatc gcctacatcc cgcttttcct gaccatctgt atgttttggg
     1321 ccgtctggtt tcagatttac accgtagcaa ccgtctattt cgacgaaacc gtcaaccgca
     1381 ccatcggttc gtttaccgtg cccgtcgcct ggaaagattc catgcaaagc ctgtgggtca
     1441 tcctgttttc cggactgatg gcggcaatgt ggacaaaaat ggggcgcaaa cagcccaaaa
     1501 ccccgctgaa attcgctatg gcggtatttg ttaccggcgc gtcgtttttg ggattcgtcc
     1561 cctttatttc cgccggcacg ccgatgccta ttgcggtttt cgcactgatt gtccttgcca
     1621 tcacgatagg cgaactgatg atttccccga ttgcgctgtc catctccacc aaaatcgcac
     1681 cgcctttatt caaaacccaa atggtcgccc ttaatttcct tgccttttca ttaggcttca
     1741 ctttgggcgg cgtattgttt gaaaaatgct atcaggcggg cgacgaaatc ggcttctatc
     1801 ggctgctgtt ctacatcggc gcagccacag gcttcctgct gctcctgctc gtccccaaat
     1861 tgaacaaaat gctcgaaggc acagactaag tcccgccccg atgccgtctg aacccttcag
     1921 acggcatttt tccgcataat gaaaccaaac cgtttccacc cgacaggaca ggctcccccc
     1981 aaccggaagg cagcctgccg attgtcattt aaatatctga ctgattccgt ctgtttgcaa
     2041 gggaaatagc cgtttatttc cgcttgggcg gaaaccggtt ctattgagta aaaggcattt
     2101 tatccgacta aattagtgct gcatcaacgg aa
//
LOCUS       DAWHXS010000170         2057 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWHXS010000170 DAWHXS010000000
VERSION     DAWHXS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.170"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..1881
                     /gene="infB"
                     /locus_tag="WIE22_001994"
     CDS             <1..1881
                     /gene="infB"
                     /locus_tag="WIE22_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ4128007.1"
                     /translation="KGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVH
                     EVLVPETITVADLAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGK
                     PAAADDPEAFLDEGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGG
                     ITQHIGAYHVETPRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIE
                     AIAHAKAAGVPMVVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGL
                     NIDALLEAVLLEAEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDML
                     LAGTAFGKIRAMVDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIAL
                     FRQGKYRDVRLAKQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLST
                     DEVKVNVLHSGVGGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNII
                     YDAIDDVKAAMSGMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVR
                     LIRNNVVIHTGELASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVAR
                     SL"
BASE COUNT          534 a          571 c          555 g          397 t
ORIGIN      
        1 aaaggcgggc gcgaccgcaa caatgcacgc aacggcgacg acgagcgcgt acgcggcagc
       61 aaaaaaggca aaaaactcaa actcgagccg aatcaacacg ccttccaagc accgaccgaa
      121 cccgtcgttc atgaagtttt ggttcccgaa accattaccg ttgccgattt ggcgcacaaa
      181 atggcggtca aaggcgtgga agtggtcaaa gccctgatga agatgggcat gatggttacc
      241 atcaaccaat ccatcgacca agacaccgcc ctgattgtgg tggaagaact cggccacatc
      301 ggcaaacctg ccgcagccga cgaccctgaa gcattcttgg acgagggcgc ggaagcggtg
      361 gaagccgaag cattgccgcg tccgcccgtc gttaccgtca tgggccacgt cgaccacggc
      421 aaaacctcgc tgctggacta catccgccgt accaaagtgg tacagggcga agcaggcggc
      481 attacccaac acatcggtgc ataccacgtt gaaaccccac gcggcgtgat taccttcttg
      541 gatactccgg gtcacgaagc gtttactgcc atgcgtgcgc gcggtgcgaa agcaaccgat
      601 atcgtgattc ttgtggtcgc cgccgacgac ggtgtgatgc cgcaaaccat cgaagcgatt
      661 gcccacgcca aagcggcagg cgtaccgatg gtggttgccg tcaacaaaat cgataaagaa
      721 gccgccaacc cagagcgtat ccgccaagag ctgaccgcac acgaagtcgt gcctgacgaa
      781 tggggcggcg atgtacagtt tatcgacgtt tccgctaaaa aaggcctgaa catcgatgca
      841 ttgctcgaag cagtcttgct cgaagccgaa gttttggaac tgactgcccc tgtcgatgca
      901 cctgccaaag gtatcatcgt tgaggcacgt ttggacaaag gccgcggcgc ggttgccaca
      961 ttgctggttc aaagcggtac gctgaaaaaa ggcgatatgc tgctggccgg tacggcattc
     1021 ggcaaaatcc gcgcgatggt cgatgaaaac ggcaaatcca ttaccgaagc cggcccgtcc
     1081 atccccgtcg aaatcctcgg cttgtccgac gtaccgaatg cgggtgaaga cgcgatggta
     1141 ttggcggacg agaaaaaagc gcgcgaaatc gccctcttcc gccaaggcaa ataccgcgac
     1201 gtgcgccttg ccaaacagca ggcggcgaag ctggaaaata tgttcaacaa tatgggcgaa
     1261 acccaggccc aatctttgtc ggtcatcatc aaggcagacg ttcagggttc ttacgaggct
     1321 ttggcgggca gcctgaaaaa actgtccacc gacgaggtga aagtgaacgt attacacagc
     1381 ggcgtgggcg gcattaccga atctgatgtc aaccttgcca tcgcttcggg cgcattcatt
     1441 atcggcttta acgtgcgtgc agatgcctct tcgcgcaaac ttgccgaaaa tgaaaacgtg
     1501 gaaatccgct actacaacat catctacgat gccatcgacg acgtgaaggc ggcgatgagc
     1561 ggtatgcttt ccccggaaga gaaagaacag gttaccggta cggtcgaaat ccgtcaggtc
     1621 atctccgttt ccaaagtcgg caacattgca ggctgtatgg ttaccgacgg cgtggtcaaa
     1681 cgcgattccc atgtccgcct catccgcaac aacgtggtta tccacacggg cgaactggct
     1741 tcgttgaaac gctataaaga cgatgtcaaa gaagtccgca tgggcttcga gtgcggcctg
     1801 atgctcaaag gctacaacga aatcatggaa ggcgaccaac tggaatgctt cgacatcgtc
     1861 gaagttgccc gcagcctgta attcctttgc aaataaaatg ccgtctgaag cgttcagacg
     1921 gcaacgaaac gggttctgta tcatacagaa cccgtttttt gtcgcaaatc ggcttcagac
     1981 agccctcttg ccttatcccg atttgaatct gacttgccat acaaacaggc ttcagacggc
     2041 attatagtga attaaat
//
LOCUS       DAWHXS010000171         1947 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWHXS010000171 DAWHXS010000000
VERSION     DAWHXS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.171"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(359..1348)
                     /gene="porB"
                     /locus_tag="WIE22_001995"
     CDS             complement(359..1348)
                     /gene="porB"
                     /locus_tag="WIE22_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ4128008.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFMQYGGAYKRHQNVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVKDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          494 a          438 c          496 g          519 t
ORIGIN      
        1 ggaatgacgg aatgtaggtt cgtaggaatg acgtgatgca ggtttccgta tggatggatt
       61 cgtcattccc gcgcaggcgg gaatctagac attcaatgct aaggcaattt atcgggaatg
      121 actgaaactc aaaaaactgg attcccactt tcgtgggaat gacgggatgt aggttttctt
      181 aaccctgagt cctagattcc cgcctgcgcg ggaatgacgg catattttga cattgaataa
      241 aaaagactaa aaacaggaaa aagccaaaca gaaaacagcg aaacagaaaa caggaaaaag
      301 ccaaacagaa aaaagcctgt ctggcgacag gctttttgtt gataccaatc tttgcagatt
      361 agaatttgtg gcgcagaccg acaccgccgg cagtcgatac gaatttgctt tcgcctttgc
      421 cttcttgcaa ccaaccggca gaaaccaagg cagaagtgcg tttggagaag tcgtattccg
      481 caccgacaac cacttggtcg taatcgttgg tgtagtctgc atcatcaaac gagcctttga
      541 agccgtgggc gtaagaaacg cggggcgtta cgttgccgaa gcggtatgcc aaggtagcgg
      601 caacttcggt ttgagagttg tgcgaattgt ctttaaccag tttcgcgtct tgttgctgta
      661 cggctacgga agcgtacagg gcatcattgt cgtaaccgct gaccaaacgg tgaatctggt
      721 atttctcaat cttcacgtta tccacattct gatgtctttt ataggcaccg ccatattgca
      781 tgaagaagcc gccgtttttg tagttgaagc cggcgtggta agattcgctg ttatgtctgc
      841 ctgcattgtc gttaagcgcg tattgtacgc tgccgctgag gccggcaaat tcgggagaat
      901 cgtagcgtac ggaaatgagg cgtgcctcgg gttcggcaat tttgtttaca cccaaatagt
      961 cgcttttgct atcccaagga ttgatgtcgc cggtgtcttt caggacgctg ttcaaacggc
     1021 cgacgcgcaa tttaccgaag ccgcctttca aaccgatgaa ggattggcgg ttgccccaac
     1081 cggagtcagt accggcgata gatgcctttt gctcaacctg ccaaatggct ttcagaccgt
     1141 taccgaggtc ttcttggcct ttgaagccga tttttgaacc caaatcaacg atgccggtac
     1201 cggtttcaac gctaaccacc tgacctccat tgtgctctac agagcgggaa gtttctacgc
     1261 cggctttgat ggtgccgtac agggtaacgt cagccattgc tgcaacagga agggctgcca
     1321 aagtcagggc aatcagggat tttttcattg ctgtattcct tttttggtta agaaatttaa
     1381 gcaggccggg ctttccaagc cgcttagctt tgcatttacc gccgacgttt tcggcgattc
     1441 catgtctgaa actatagatg ttcctgtctg aaaaaacaaa cttctgcctg tttttgtgtg
     1501 gaattcgatg tgtattttga agggcggata gagatattgt gggtattttt tgttttataa
     1561 catatggtta tttaaatttt ttaagatttg catttttaca acacttactc gggagggtat
     1621 tggagggcat tgcaaaccga gggttataaa gacggcaaaa aacgcgcacg tggtttcttt
     1681 cggaacgggc ggatgcaaaa cccgcccctg cggcaggaga tagtggatta acaaaaatca
     1741 ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt
     1801 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgctaac cgtcattccc
     1861 gccacttttc gtcattcccg ctcaggcggg aatctagaat ctcggacttt cagataatct
     1921 ttgaatattg ccgctgcctt aaggtct
//
LOCUS       DAWHXS010000172         1945 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWHXS010000172 DAWHXS010000000
VERSION     DAWHXS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.172"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(195..1352)
                     /locus_tag="WIE22_001996"
     CDS             complement(195..1352)
                     /locus_tag="WIE22_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ4128009.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     PNIQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGASQWG
                     NRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVSVR
                     YDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQDKKGQPPTLVPAVVGKPGSDVYYAGL
                     NYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYEE
                     GGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGE
                     NTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          508 a          495 c          457 g          485 t
ORIGIN      
        1 tatatgtgca aaccaagcca aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaaa gcaagccgtc aaaaacaaag ctatccgctt
      181 taccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgc
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgcggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcggcattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcaggcggt gtacctgatg gtttttcaag ggatcggtac ctttggcttc atcactcccg
      601 ctgccgagca agaacaactc aaaagcatca cggcccacat tggcgtgttt cgcgtattta
      661 aaggcatagt tcccggcaaa accgccattt ttgtaattca gaccggcata atacacatcc
      721 gaccccggct tgccgacaac agccggaacg agagtaggcg gctgaccttt cttatcctga
      781 acaaaatgag ccggcgtata ggcggacttg ctgttttgag ccggaacgaa ttggacgctg
      841 ccgctgaaac cggaaaattc gggggaatcg tagcgtacgg aaaccgacat atcgtcgtgg
      901 cgtttgaaaa tacccaattg cgaagccaca tcattgttgc tgtcccaagg atcaatggct
      961 tggctggcat cgtcaaactg attcgcaacg cgaccggcgc gcagcgtgcc gaattcgcct
     1021 gccaagccga taaaggattc cctgttgccc cactgggacg cgccgccgcc ggcaacggat
     1081 acgtcttgct caagctgcca aacagccttc agcccttcgc ccaaatcctc actcccctta
     1141 aagccgataa acgagccgaa atcgctgatt ttcgtcctga tgcggctttt gcgcttagta
     1201 acctgaggtt gaatatttgg gagcggttcg gtcaactgca gctggatgtt cctgccttcc
     1261 acgccggctt tgatttcgcc gtacaggctg acatcggcaa cggccgcaag cggcagtgcg
     1321 gacaatacga gggcggtaag tttttttcgc atatcggctt ccttttgtaa atttgataaa
     1381 aacctaaaaa catcgggcaa acacccgata tgtcatcaat tatacccccc ccccgcaaaa
     1441 aaccattttt cagaacaaat atctgataaa tgccgcaacc tttattttaa aaatgattat
     1501 attttgatat aaaacaatag cttatttttc aaaaaacgtt gtgtttctac aacacaattc
     1561 aagcgcagac ctcgtgcggg ccgatgcgct gctgcccgga tgcagtctcg gctttttaaa
     1621 acgccataaa aaaacacacg cggcacttta tagtggatta acaaaaacaa gtacggcgtt
     1681 gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt
     1741 tccgtactat ttgtactgtc tgcggcttcg tcgtcttgtc ctgatttttg ttaatccgct
     1801 ataaagaccg tcgggcatct gcagccgtca ttcccgcgta ggcgggaatc tggaatttca
     1861 atgcctcaag aatttatcgg aaaaaaccaa aacccttccg ccgtcattcc cacgaaagtg
     1921 ggaatctaga aatgaaaagc aacag
//
LOCUS       DAWHXS010000173         1854 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWHXS010000173 DAWHXS010000000
VERSION     DAWHXS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.173"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..439
                     /locus_tag="WIE22_001997"
     CDS             <1..439
                     /locus_tag="WIE22_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128010.1"
                     /translation="YLGVDGQQAENASAKNPTLPAANEMARIEGKSLIVFGDYGRNWR
                     RTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTEQKPGLFKRLLGKGKAEKPAEQ
                     PELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGKLHSELR"
     gene            complement(496..1428)
                     /gene="pip"
                     /locus_tag="WIE22_001998"
     CDS             complement(496..1428)
                     /gene="pip"
                     /locus_tag="WIE22_001998"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ4128011.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKDWADWESYLICFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT          501 a          567 c          398 g          388 t
ORIGIN      
        1 atatttgggc gttgacggac agcaggcgga aaacgcatcg gcaaaaaacc cgaccctgcc
       61 cgccgccaac gaaatggcgc gtatcgaagg caaaagcctg attgtctttg gcgactacgg
      121 cagaaactgg cggcgcaccg cgctcgccct cgaccgcatc gggctgaccg tcgtcggtca
      181 aaacaccgaa cgccacgctt tcctggttca aaaagccccg aacgaaagca atgcagttac
      241 cgaacaaaaa cccggcctgt tcaaacgcct gctgggcaaa ggcaaagcgg agaaacctgc
      301 cgaacagccg gaactgattg tctatgccga gcctgtcgcc aacggctcgc gcatcgtcct
      361 gctcaacaaa gacggcagcg catatgccgg caaagacgca tccgcattat tgggcaaact
      421 ccattccgaa ctgcgttaaa ccattcaaaa caaaagcagc ttccaaacag aagctgcttt
      481 tttatgcgga actttttaca acaaatgggg caaaatatcc tcaactgcct gaaccaacgc
      541 atccaccaaa ggcggatcga acgcacgatg ccctgcctga accaccctca attccgcttc
      601 gggaaaggct ttcgacagcg cccacgcact ctgcatcggc gtacacaaat cataccgccc
      661 ctgcacgata atggtcggga tatgctgtat tttgccgata ttgttcaaaa tcgccctatc
      721 gccctgcaac cagccgccgt tgacaaaata atggttttcc aaacgcgcga ttgccagcga
      781 ggcataagca tcttcatcca cttcctccgg ctcgaaacag atcagatagc tttcccaatc
      841 cgcccaatcc ttcgcggcag acaggcacac ttcttcatct tgatgaaaca gcaacccgtg
      901 atacgcctca atcagccggt tccgccgatt ttcagcaatc ggcgcgacaa atttttgcca
      961 ttgttccgga taaatccggc tcacaccgcc cgcctcgttc agccacgccg tttcagacgg
     1021 cctgcacaaa aatatcccgc gcaacaccaa tccctttacc cgttccggat gggtttgggc
     1081 ataagccagc gacaaagtgc tgccccacga accgccgaac accagccatt tcccgatacc
     1141 cagcatttca cggacttttt caatatccgc caccaaatcc caagtcgtat tgtcttccgc
     1201 acaagcatac gggcgcgaac gtccgcaacc gcgctggtcg atgatgacga tgcggaacac
     1261 atcgggatta aaaaaacccc gacattcagg cgaagccccc gcgcccggcc cgccatgtaa
     1321 aaaaataacc ggcacaccgt cgggattgcc cgattcctcc caataaattt gatgaatttc
     1381 agacacctgc aagtatccgc tatgaaaagg ctgttttatc tcatacatca tcttctccca
     1441 tattgcttct gataatccga acattcccac acgcaacatc acgcacgaga cacaagccgc
     1501 cccgaaaggc aaatgccgaa aaatcgcgat atagtgaatt aacaaaaatc aggacaaggc
     1561 ggcgaagcct cagacagtac agatagtacg gaaccgattc acttggtgct tcagcacctt
     1621 agagaatcgt tctctttgag ctaaagcgag gcaacgccgt actggttttt gttcatccac
     1681 tataacagca accctgtcgc cgtcattccc gcaaaagcgg gaatccagtc cgttcaattt
     1741 cggtcatttc cgataaattc ctgctgcttt tcatttctag attcccactt tcgtgggaat
     1801 gacggcggaa gggttttggt tttttccgat aaattcttga ggcattgaaa attc
//
LOCUS       DAWHXS010000174         1758 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWHXS010000174 DAWHXS010000000
VERSION     DAWHXS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.174"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(44..367)
                     /locus_tag="WIE22_001999"
     CDS             complement(44..367)
                     /locus_tag="WIE22_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ4128012.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEIFA"
     gene            complement(355..594)
                     /locus_tag="WIE22_002000"
     CDS             complement(355..594)
                     /locus_tag="WIE22_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ4128013.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(717..1514)
                     /gene="truA"
                     /locus_tag="WIE22_002001"
     CDS             complement(717..1514)
                     /gene="truA"
                     /locus_tag="WIE22_002001"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ4128014.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALENALAQI
                     AGEAVSTIVAGRTDTGVHATAQVVHFDTAAARRQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          401 a          416 c          499 g          442 t
ORIGIN      
        1 tgttaatcca ctataatgaa cacaatccat tcagactatt caatcaggca aatatctcct
       61 gcaatactgc aaacagtttt tcagccgtac tgttgtctaa atttccaaga tgtttgacca
      121 atccggcttt atccacagcc ctaatctgtt cgggcaaaag caaaccgtct ttatcctgaa
      181 agcggacatt gacgcggaac ggggcaggac ggcttccgct cgtcatggga acgatcagca
      241 cagtcttgag atagttgtgt atttcaggag gagatactac gacacaagga cgtgtctttt
      301 tgatttcgct tcctacggtc gggtctaagg agaccagata gattccgccg cgtactacca
      361 tatccattct ttatccgctt cgttttcaat ttcggaaaaa aaatgctcct gctcggtttc
      421 gacaagcatt gcggcagctt ctgcccatcc cctgcgaacg gtaggacagc ttaaaataat
      481 attgcccttt tcaactgtaa cagccaagct gtctactgcc cctatttgac ccaataatga
      541 tttgggcaga atcacgcctt gcgagtttcc cattttgcgt atgttgagaa tcatatacgt
      601 accatattca ccagtttatg taataacaat gttagtacct tgatgaggta gtgtcaacat
      661 ggaaaaagat tgcctgacag tttgtccgat ttcaaaatct ccgcgacaag aatgttttaa
      721 agccattcgg ggatttgggg gcggatgatg ccgtatgcct cgggatagtc gacgccggtc
      781 aggtaaagcc cgtcgggcat aaaggtcggt ggggctttga ggcgactgcg ttcttgaatc
      841 agtgcggcga agccttcgac gctgagtctg ccgctgccga catagacgag cgcgcccatg
      901 atgttgcgta ccatatggtg caaaaaggcg ttgccgtgca aatcgaggcg gacgagtcct
      961 gcgctttggg taaggtcggc gcggtagatg gttttgacgg gggattttgc ctggcattcg
     1021 gcggcgcgga agctggagaa gtcttgttcg ccgatcaata aggcggcagc ctgccgcatc
     1081 tgtccgatgt cgagtttgag gtgtgtccag cctgccctgt ttttgagcag gggggaacgg
     1141 acgggggcgg attcgagcag gtagcggtaa tgccgtccgt atgcgtcgaa gcgggcgtga
     1201 aattcggggg cgacctgtcg ggcgtgcaaa acggcaatgc cttcgggcag gtgggcattt
     1261 acgccgcgca cccatgcctg ttggcgacgg gcggcggcag tatcaaaatg aacgacttgg
     1321 gcagtcgcat gaacgccggt gtcggtcctg ccggcaacaa tggtggaaac cgcttcccct
     1381 gctatttggg caagtgcgtt ttccaatgcc gcctgaacgg tcggtacgcc gtcagcctgt
     1441 ttctgccagc cgtaaaagcg gctgccgtca taggataggg ttattgccca gcgttgtttt
     1501 tgtgcggtat ccatcggatt tgggattcgg ataaatgttc agactgcatt gtatcgcaga
     1561 ttttgcaggg aaacggcaaa tgcccggggc gcgcggcgtt gtttggggag ttgggggggg
     1621 gtggatgcag ttgctacgaa tcgctatcct gtgaattcac cctgtcagga gtgcccgaat
     1681 cgtcattccc gcgcaggcgg gaatctagga cgtaaaatct aaagaaacca ttttatagtg
     1741 gattaacaaa aaccagta
//
LOCUS       DAWHXS010000175         1739 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWHXS010000175 DAWHXS010000000
VERSION     DAWHXS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.175"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            177..1151
                     /locus_tag="WIE22_002002"
     CDS             177..1151
                     /locus_tag="WIE22_002002"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ4128015.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALCGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(1310..1528)
                     /locus_tag="WIE22_002003"
     CDS             complement(1310..1528)
                     /locus_tag="WIE22_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ4128016.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT          479 a          483 c          371 g          406 t
ORIGIN      
        1 aatttatcca ctataagaat aaatatcccc tcatccgcct gccgcgttcc gacttgcccc
       61 gcgccgtttt tttccactac aatcccacgc gatatctggc ggaacgcacc aaaggaacgc
      121 cccgcagcaa tctgccgcct ttcagacggc acaaacattt ccgaaacgag caaactatgg
      181 acacactgac ccgattcctc cccgaacacc tgcaacaaaa ccagctgccc gaagcactct
      241 gcggcgtttt actgtccgtt gtttccgcct gcaccgaaat caacgccaaa gtccgcctcg
      301 gcgcactggc cggcgtattg ggtatggcgg gcacgggcaa tattcagggc gaagaccaaa
      361 aaaaactgga tgttatcgcc aacaacatca tgattgacac actcaaagcc aaccctgccg
      421 ttgccggttt ggcaagcgag gaagaagact ctttcgtaag tgccggtgaa aacggacgct
      481 atctcgtcct attcgatcct ttagacggat cgtccaatat tgatgtcaac atttccgtcg
      541 gtacgatttt ctccatcctt gaaaaacccg aaggcgcatt ggcaaccgaa tcattccttc
      601 aaacgggcag acagcagctt gccgctggtt acgttcttta cgggcctcaa acccagctcg
      661 tattcacatt cggacacggc gtatatatgt tcacgctcaa tgccgaaaac gaatttgtgc
      721 tgacaaaaga aaaaccgaaa gtacccgaaa gtaccaaaga atttgccatc aatatgtcca
      781 accgccgcca ctggctgccc cccgtccaac aatacatcga cgagctcttg gcgggcgaaa
      841 ccggcacgcg cggcaaaaac tacaatatgc gctgggtagc cagcatggtt gccgaaatcc
      901 accgcatcct gatgcgcggc ggcgtgttca tgtatccgca agacaaacgc gaccccgcca
      961 agcccggcaa actgcgcctg atgtacgaag ccaaccctat gagcctgata ctcgaacagg
     1021 cgggcgcatc ggcaagcaac gcatatcaag ccatgctcga catacagccc gaaaacctac
     1081 accaacgcgt cgccgtcatt atgggcagca gcgaagaagt ggattacctc aaccgcctgc
     1141 attcaaaata aaaggcagcc tgaccgataa agaaaggcgg cagcgcgcat ccgacgctgc
     1201 cgccgatatt tcgtttatag ccttaaaaag atagaaaacc acctttccga acggcaaaaa
     1261 tgccgtctga aaagttttca gacggcattt taaaaattaa agcccgaatt tatttgcccg
     1321 ctgctttttg caggatggcg tacaactcgt ctttcagttt caatttggct tttttcagct
     1381 catcgatggt ttccgcaccg ctggtaaccg gattgttgac cagaccggta attttatcgt
     1441 ccagctcgtt gtgttcgtcg aacagacggg cgaagcggga attttcctgt ttcaatttgg
     1501 aaatcaaatc acggtattct gggaacatat cgctttcctc tcttggttta cggttaaaaa
     1561 gtcaaattga atctttacat taattataac aaagataata tttttatcca gtgttttatc
     1621 ggatacggca aaattttctt ttaacaccgc cgtccggttg ataaaaacgt tgacaatatc
     1681 acgctttacg cataaatttt cagcctttcc tactgtatag tggattaaca aaaatcagg
//
LOCUS       DAWHXS010000176         1733 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWHXS010000176 DAWHXS010000000
VERSION     DAWHXS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.176"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            355..1659
                     /locus_tag="WIE22_002004"
     CDS             355..1659
                     /locus_tag="WIE22_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ4128017.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          357 a          432 c          510 g          434 t
ORIGIN      
        1 attaaaaatt tttaatatat taatcaataa attaatttta taaaataaaa atattgtcaa
       61 caatattttg ccttatcgcc caaacctctg tatattttcc tacagtaaat tgttgacaat
      121 ccatacgccc acatatgcgc cgcctaagga taaatcctcc cgccggacaa cgggtgcaag
      181 ggattggatg cgatatttcc atattcaaac aagggatttg tttcacgcac agggcggcac
      241 atcggcaaaa tccccgcgcc gccccggtcc ggcagggctt gcgtccctcc cggacaagcc
      301 ccgaccccgc ctttccgaaa gacgggctca accattaagg aaactttaat caaaatgaaa
      361 aaacacatat gggcggcatc tttgctgccg gcatccctat cggcagaacc tttaaactgg
      421 tggaagcctt attccgccgt caattcgggc gataccgcct gggtgatgac tgcggctgcc
      481 ttggtactgt tgatgacgct tcccgggctg gctttattct acggcggtat ggtgcggaaa
      541 aaaaacctgc tctcgacgat gatgcacagc ttttccatcg cgacattggt gggaatcctt
      601 tgggtcgccg tcggctattc tttagcgttc acgccgggaa atgcctttat cggcggtttg
      661 gggcgcgtat ttttaagcgg gatgcagata gacgctgccg cacagatgct gaccgtgtcg
      721 cccaatgcgc cgactgttcc cgaaccggta tttatgtttt ttcagatgac gtttgccatt
      781 atttcgaccg ccattattac cggcgcgttt gccgaacgga tgaaatattc ggcaatgatg
      841 ctgttttcgg gcatatggtt tttattggtt tatgtgccgg gcgcgcattg ggtctggggc
      901 ggcggcttta tgagcaaggg cggcgtattg gattatgccg gcggtacggt ggtgcacatc
      961 aatgccggta tcgcgggact cgtcgccgcc ttggttttgg gcaggcgcat aggctacggg
     1021 cgcgaggcga tgcctccgca caatatggcg atgacactga tcggcgcggc aatgttgtgg
     1081 ttcggctggt tcggctttaa cgccggatcg gcgcttgcgg cagacgcggc ggcgggtatg
     1141 gcgatggcgg taacgcaggt gtcggccgta ttcggcgcgg caggctggct tgcctgcgaa
     1201 aaaatagcgg gacacaaacc ttccgctttg gggctggctt ccggcgcggt ttccggtctg
     1261 gtcggcatca cccctgccgc cggctttacc ggcccgtctg gagcggccgc catcggtata
     1321 ttgactgccg ccgcgtgctt tgtgtccgtc accgtcgtca aacacaaatt gcgttacgat
     1381 gattctttgg acgctttcgg catacacgga ttcggcgggc tggtgggcgg aatattgacc
     1441 ggcatctttt tcgacaaccg cattttcggc ggggatgcgg cagtttggca gcagttgtgg
     1501 atacaggtaa aagacgggtt cgttatggcg gcatacagcg ggctgatgag ttgggcgatt
     1561 ttgaaggtcg tggggaaaat ctgcggcggc ctgcgcgtcg gcaaggatgt cgaacgcgaa
     1621 ggtttggatc tgaatatcca cggagaacgc gtggaataag ggcggctatg ccgtctgaag
     1681 cctgaaaatc gggtttcaga cggcattttt cacgtttgcc gccgatggat aaa
//
LOCUS       DAWHXS010000177         1731 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWHXS010000177 DAWHXS010000000
VERSION     DAWHXS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.177"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..527)
                     /locus_tag="WIE22_002005"
     CDS             complement(<1..527)
                     /locus_tag="WIE22_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ4128018.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGN"
     gene            complement(1529..1729)
                     /locus_tag="WIE22_002006"
     CDS             complement(1529..1729)
                     /locus_tag="WIE22_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128019.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT          558 a          410 c          259 g          504 t
ORIGIN      
        1 aaatttccct cacgggtttg ccaagcatcc aaaaactgcg ccctgctcat cgaaacatga
       61 cccagcgacg ggtcggcaag caaaaccgta ttgccgtcta taccgcgcaa taccgagaaa
      121 tgatcatcct tacggtattt cagatacacg atgacgggga ttttcaactg cgcgagctgc
      181 tcgaaagaca gggcatagcc tttcgcttca aaacccaaat ctggcataat gcgccgcata
      241 tcctcaaacg acgcgcgcat ctgctcctta tccagctttt tcaacacttc ttcttccgtc
      301 agcgtttgcc cgtaaaaatt gttcaaaagc gtcgccaccg aagccgcccc gcaggaaaaa
      361 tccaaatcct gctttacaat attgaaatcc cgccgcgctt tccaactctg cactttgatt
      421 tttccgtaaa caacaggatt atcgttaaac atcggtgcag cattcagacg ataagataaa
      481 gaaacgacaa cacacgccaa cagaaaaaca tatttgaact tcatcatatt gtccacataa
      541 agggcagcct gaaaatcttt caggctgccc ttgtcaaatt attcctagct ttcggctttt
      601 ttggcaaacc aaacaatccg attacccgca taatactttc catttattga aatccgacaa
      661 gccgcgccca aaaatgccat gcactgtcga tttccgcagc aatctttgta ccgttttctt
      721 caaattccaa atattcaccc aataataaac ttgaaacaga acgcgtgggc agcaagtgcc
      781 caccctacgc ttattcaaat aatttgatta aataaagaat aaagaaaatg catcaggaac
      841 aaaattataa tctgccacct gactcacacc gcttttaaag taaggggcat caaaatcaaa
      901 accgcaaaaa aaataatttt tgcattgatt tttaatagat ttaaaattcg aatatagtgt
      961 ttctccagat ttaaaaagtt tatctttatc tatatattga tgctttccct atccaaaata
     1021 aatattcaaa cattaaaaaa tcattacatg accaagccaa atcataaatt tgattcaaat
     1081 gggtatcata aagataaaaa taataaggtt tgggaacagg taaaatattt aatggaaaag
     1141 gaggactaat tttcttaata tctgatgact gatatccata tctttctata ttcttaaaaa
     1201 tttcatcttt cttaagataa caagacattc tgaagataac aagacattct gacaatcaca
     1261 acctgtttat cggatatatt atctatttgc atggcgcgta taacacgcca tgcctgatta
     1321 aaattagtct cccttacctt agaatctatt atcttttcca aagatgaaaa tgccccttat
     1381 cttgaactct ccagttagaa cctggaagtt tcgtacctct taaatgtgta ttaatatttc
     1441 ttatagtttc attaaaatgc cacgcgctgc cgatttcaac ggtaattttc gtaccgcttt
     1501 cttcaaattc caggtattcc cccattagct aacgcaaaga agcagacgcc atttcggctt
     1561 cgttatgata aacccgcctt ccgttgatat agacttccgc ccctgtccgc tccaaattcc
     1621 aaaaattccg tactgaaatt tccatatccc gatattgtgc agaccatgtt ttttcgaagg
     1681 ttttcataaa atttcctata cctgtccaat cggcacatat caattgcatt a
//
LOCUS       DAWHXS010000178         1633 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWHXS010000178 DAWHXS010000000
VERSION     DAWHXS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.178"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            50..244
                     /gene="thiS"
                     /locus_tag="WIE22_002007"
     CDS             50..244
                     /gene="thiS"
                     /locus_tag="WIE22_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ4128020.1"
                     /translation="MNIILNGEPAELHGTTVADLIAQTAPQKPFAVAVNTGFVAKGAY
                     AETVLNENDKIDIVRPVVGG"
     gene            458..1246
                     /locus_tag="WIE22_002008"
     CDS             458..1246
                     /locus_tag="WIE22_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ4128021.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT          394 a          481 c          435 g          323 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataaat acagaaacat cgagaaacca tgaacatcat
       61 cttaaacggc gaacccgccg aacttcacgg cacgaccgtt gccgacctca tcgcccaaac
      121 cgcgccgcaa aagccttttg ccgtagcggt gaataccgga tttgtcgcca aaggcgcgta
      181 tgcggaaacc gttttgaacg aaaacgacaa aatcgatatc gtgcggccgg tggtcggcgg
      241 ctaggcggtt ttgccttttc agacgacccc tgtccccaaa acaacgttat ggtggattaa
      301 ctttaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca
      361 cttggtgctt cagcacctta gagaatcgtt ttctttgagc taaggcgagg caacgccgta
      421 ctggtttttg ttaatccact atacaaagga acccattatg ctcaccctat acggagaaac
      481 tttcccttca cggctgctgc tcggcacggc tgcctacccg acccctgaaa tcctcaaaca
      541 atccgtccgt atcgcccaac ccgcgatgat taccgtctca ttgcgccgcg cgggaagcgg
      601 cggcgaggcg cacggtcagg ggttttggtc gctgcttcaa gaaaccggcg ttcccgtcct
      661 gccgaacacg gcaggctgcc aaagcgtgca ggaagcggta acgacggcgc aaatggcgcg
      721 cgaagtgttt gaaaccgatt ggataaaatt ggaactcatc ggagatgacg acaccttgca
      781 gccggatgtg ttccagcttg tcgaagcggc ggaaatcctg attaaagacg gcttcaaagt
      841 gctgccttat tgcaccgaag acctgattgc ctgccgccgc ctgctcgacg cgggctgtca
      901 ggcgttgatg ccgtgggcgg ccccgatcgg cacgggtttg ggcgcggttc acgcctacgc
      961 gttgaacgtc ctgcgcgaac gcctgcccga cacgccgctg attatcgacg cgggcttggg
     1021 tttgccctca caggcggcac aagtgatgga atggggcttt gacggcgtgc ttttgaatac
     1081 tgccgtttcc cgcagcggcg atccggtcaa tatggcacgc gccttcgcac tcgccgtcga
     1141 atccggacgg ctggcatttg aagccggacc ggtcgaagca cgcgacaaag cgcaagccag
     1201 cacgccgaca gtcggacaac cgttttggca ttcggcggaa tattgaaaaa ggcggcaaaa
     1261 atgccgtctg aaggcttcag acggcatcgc ggtccaaaac ggcggcggcc tgaaacggac
     1321 aaaccgccat tccccagcat cacggctttg tcggaaaaaa tggaaaaacc ggccgaaaaa
     1381 ccttgccgcc cgtcccgatg ccgcaaccaa cgaaacactc ggcctccacg gtgtgcaggc
     1441 tgccgcgcaa gccctaaata cggcaataga agaagcgttt tgttgttgtt tgaatacact
     1501 taaaaatacc taaagccgtc cgtaagcctt catccgcaac ggttttaccg cttttgcatc
     1561 cccgaatcca cgctcaaaca cccccgaatg acaaccctgt ccgcgcccaa tcggacggat
     1621 gttcaaacac ggg
//
LOCUS       DAWHXS010000179         1620 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWHXS010000179 DAWHXS010000000
VERSION     DAWHXS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.179"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(20..682)
                     /locus_tag="WIE22_002009"
     CDS             complement(20..682)
                     /locus_tag="WIE22_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ4128022.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(695..1522)
                     /locus_tag="WIE22_002010"
     CDS             complement(695..1522)
                     /locus_tag="WIE22_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ4128023.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          300 a          434 c          506 g          380 t
ORIGIN      
        1 tttgtggggc ggatattttt cagggctgta ttttgtccag acattcgagc agatcgagtg
       61 gcgtgcggat gtggaaatcc gcctgccatg agccggtatc gtcttcggga gcgatgtagc
      121 cccattcggc gaggacggtt ttcatgccgg cgttgcggcc tgcttggatg tcgcgttcgg
      181 catcaccgac gtagagtgtg tgttgcgggt cggcgtggat ttgtccgcac gcatacagca
      241 tgggtttgac gctgggcttg ggttcgccgc aggtgtcgcc gctgacgacg acggcgggtg
      301 ggatgatgaa gccgagtttg ggggcgagtt tgtcggtgaa gcgcatgggt ttgttggtga
      361 tgatgcccca tttgatgccg cgtctgtcga gttcggcgat gagttcgttc acgccgccga
      421 agagggtggt gtcttgggcg tagcggctgt cgtattcgtc aaggtattcg gtgcgccatc
      481 gggcatagtc ggggtggtcg ggggtgatgc ctgcgccgag tttgatcagt cctgccgcgc
      541 cgtggctggc ttgggtgcgg atctcgtcca tgctttttgc aggtagtccg tggcgagcga
      601 gcagggtgtt gagtgcgcct ccgaggtcga gggcggtgtc ggcaagtgtg ccgtcgaggt
      661 cgaagagtac ggcgtgtatc atgtgtgttc cttttcagac ggtatttttg ttctgtttca
      721 aaccctgctc gaaatcttcc agcatatcca attcaaagcg gctgaagccg gctttttcgc
      781 gcgcttcgat gttcacatag ccacggaaga taaacatatc gtaacgggca atcagactgc
      841 ggaacagggc aatcggttcc aaaccgcgtt cgcggcagag gtgttgatac caccggttgc
      901 cgatggcgac atggcccact tcgtcgcggt aaatgatgtc caatacgccg caggtttccg
      961 aatcgccgcg ctgcgccacc ttcgcgcgta tccccggcgt aacgtccagc ccgcgcgctt
     1021 ccaacacgcg cggtaccaac gccatacgca acagcgggtc gtaggcggtt ttgtacgcca
     1081 tatcccacaa atggttgtgc gcctcgaaac tgccgtaatc gaagccgaaa gcgcgcaatc
     1141 tgtcgcgcat caggcggaaa tggtacacct cttccttcgc cactttcacc cagtcgcgga
     1201 caaactgaaa cggcagcgtg cggaaacggt atgccgcgtc caaagctaga ttgacggcgt
     1261 tgaattcgat atgcgcaatc gcgtgcagca tcgccgcata accttcggtt gtgttcattt
     1321 tgcgcggggt cagttgcgac ggcgcgacca aaacaggctt gtccggccgt cccgctcggg
     1381 ggaagtccgc cggcggcgcg tttgcttccg ccccgtccgc attttgaacg gcggcaaacg
     1441 cctcatccgt cagccgtcct ttttcatctg ggtcgcccga aagcagggcg cgttccagca
     1501 aagcataaat atcgagtttc atctcaagtc cgccgtgttc ggaaaacaaa tattatagcg
     1561 ttttaaaaaa acaagatgag gcatataatc tccgcgattc ggcattccgc gcccaaaccg
//
LOCUS       DAWHXS010000180         1603 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWHXS010000180 DAWHXS010000000
VERSION     DAWHXS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.180"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            583..1506
                     /locus_tag="WIE22_002011"
     CDS             583..1506
                     /locus_tag="WIE22_002011"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ4128024.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          417 a          384 c          372 g          430 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataattt attgaagatg gctgaaattc aaagctacag
       61 aaatttataa acaaaaaaca gcaccccgcc gccgtcattc ccgcaaaagc gggaatctag
      121 acattcaatg ctaaggcaat ttatcgggaa tgactgaaac tcaaaaaact ggattcccac
      181 ttccgtggga atgacggaat gtaggttcgt gggaatgacg ggatttaagt ttcaaaattt
      241 attctaaata actgaaactc aacgaactag attcccgctt ttgcgggaat gacgaatttt
      301 aggtttctgt ttgtggtttt ctgttttggt tttttgtcct tgtgggaatg acggttcacg
      361 cgaaaatgcc gtccgaaagg ttttcggacg gcattttttg cgtttttcag gaggggggcg
      421 gcttcgtcgc cttgtcttga tttttgttaa tccactatac atttttatgc ggcttacgta
      481 ttgaaaacag atacattggc aaatactatg gaaaaacata gcttgttttt ccggttttat
      541 cgtattattc caaaacttac cccattgata aggaagcggt ttatgctgca aagaactttg
      601 gcgaaatcca tcagcgttac cggagtcggg ctgcattcgg gcgaacgcgt cgcgctgacc
      661 ctgcaccccg cgcctgaaaa cagcgggatt tccttccgcc gtaccgattt ggacggcgag
      721 atgggcgaac aaatcaagct gaccccttat ttgatcaacg atacccgcct ttcctccacc
      781 atcgttaccg acaaaggcgt gcgcgtcggc acgatcgaac acattatgtc cgcgctgtcc
      841 gcctacggca tcgacaatgc gctgattgag ctgaacgcgc ccgaaatccc gattatggac
      901 ggctccagcc tgccgtttat ttaccttttg caagatgcgg gcgtggtcga tcaaaaggcg
      961 caaaagcgtt ttttgaaaat cctcaagcct gtcgaaatca aagaagcggg caaatgggtg
     1021 cgctttacgc cgtatgacgg ctttaaggta acgctgacca tcgaattcga ccatccggtt
     1081 ttcaaccgca gctcgcccac ttttgaaatt gatttcgccg gcaaatccta catcgacgaa
     1141 atcgcgcgcg cgcgcacttt cggctttatg cacgaagtgg aaatgatgcg cgcccacaat
     1201 ctgggtttgg gcggcaattt gaacaacgct atcgtgattg acgacacgga tgtcctgaat
     1261 cctgaaggct tgcgctatcc cgatgagttt gtgcgccaca aaatccttga tgccatcggt
     1321 gatttgtata tcgtcggaca tccgattgtc ggcgcgtttg aaggctacaa atcgggacac
     1381 gccatcaaca acgcactctt gcgcgcggtt ttggcagacg aaacggctta cgaccgggtg
     1441 gaatttgccg acagcgatga tttgcccgac gcatttcacg agctgaacat cagaacttgt
     1501 ggataacgct ttctattttt tccggtaaat tcctaaaact taaaatttca tcattcctac
     1561 aaaaacagaa aaccaaaatc agaaacctaa aattcgtcat tcc
//
LOCUS       DAWHXS010000181         1597 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWHXS010000181 DAWHXS010000000
VERSION     DAWHXS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.181"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..905)
                     /locus_tag="WIE22_002012"
     CDS             complement(<1..905)
                     /locus_tag="WIE22_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ4128025.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALASVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKFSTRAVPYVGTALLAHDVYETFKEDIQAQGYQYDPETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDCVVNKGDDYRNGADFYLSRNQKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATG"
     gene            complement(1252..1554)
                     /locus_tag="WIE22_002013"
     CDS             complement(1252..1554)
                     /locus_tag="WIE22_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128026.1"
                     /translation="MSFRVGMNCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGEKW
                     VLNGNPVTLAYPKCSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
BASE COUNT          508 a          362 c          250 g          477 t
ORIGIN      
        1 ccggttgcct ttatgtattt gtcgggattg gcatcgactt tcaacgataa aatctcttcc
       61 agctttttgg catccatttc ttctttgtat ttctgattgc ggctaaggta aaaatcagcc
      121 ccatttctgt aatcatcacc tttattgacc acacaatctc cgccgttcca atcaaatgtg
      181 caacgattta aaacaaaatt attccaatcc aaagaactta atttattcag ttcttcttta
      241 tgccaattcc aaaacggacg tgccagccta tacatttggc tttccatcaa ttctttgact
      301 tcggggaatc tgctgtaatc ggacataagg cgcataatcg aactgtcaac gccgtagcag
      361 ccataggttc tattaatacg tctttcgtct tcgtaccaaa ggcaattact atattcgtag
      421 ccttttacaa atttgtcggt ttcggggtcg tattggtagc cttgtgcctg tatgtcttct
      481 ttgaaagttt cgtatacgtc gtgggctaaa agggctgttc cgacataggg aaccgccctt
      541 gtgctgaatt tcgcgcctaa gcgggcaagt ttgccgactc ctgccaatac gccggcgcgg
      601 gaaactgatg aagatacaac cgaactaact ttaccagtac ttgatgctcc agtagataca
      661 tgcctaactt ttaaatcttg agcttctgaa gttaaatatc ttcccgattc tctatcaaat
      721 acacctttcc aaattcttct tcttttgtca tctgaacctc gaatacctaa atatctgcca
      781 tcatcataaa cttcaaccct atcaaattta cccggaacat ttactgatgc caatgcaaat
      841 ttaggtacaa aaaaactaaa gcataggata atctttaaaa aatttcgccc taggataaga
      901 aacataacat tttgcttcat ctaatttaat taataatgta gaagaatcag ggaattgaat
      961 ttctataaaa tctctatcat aacgatgaaa gaaatttcta aaaatatcaa atgaaaacga
     1021 agatttaaaa accccttttc taatcaattc cctataataa attaacgctt gtggtaaatc
     1081 tctataaata tgtgaatcag tttgaaattt aactaaatga tattcagaaa taaacataat
     1141 tcggaccttt taaaacactc taaaaagccg attctaccaa ccgttaccaa aacagccaag
     1201 caacagacaa agccaagaag aaacctagaa gaaaccaaaa atcaataacc atcaatcagt
     1261 accaaccttg cctatgtctt ttaaaaaatt aatcaaaagc ctgaagccgt aaatgactac
     1321 gaacagaatt aaaaccgttg aacctacata agaaccctgt ttgatctgct caaaattaga
     1381 acatttcgga taagccagcg tgaccggatt tccgttcagc acccattttt cgcccaccct
     1441 ttccggcctg atgatttttc cgtcctgggt aacggtaggg ggaagggacg acaataaata
     1501 gtcgtctgcc tgcaatcttg tatcaaaaca attcataccg acacgaaagc tcatttacgc
     1561 gccccctctt tcttcactgc cgttatttga cagattt
//
LOCUS       DAWHXS010000182         1583 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWHXS010000182 DAWHXS010000000
VERSION     DAWHXS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.182"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            59..1432
                     /locus_tag="WIE22_002014"
     CDS             59..1432
                     /locus_tag="WIE22_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ4128027.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLHRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKLNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          409 a          519 c          386 g          269 t
ORIGIN      
        1 cccgtcctat cggtttcccg tttcagacga cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttgcta gccgccgcgc aaaccggcac aggcaaaacc gccgccttta tgctgcccag
      241 tctggaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtc ctcaccccca cgcgcgaact tgccgaccaa atcgaccaaa acgtgcaggg
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgcg ccggctgcga aatcgtcgtc gccaccgtcg ggcggctgct
      481 cgaccacgtc aaacaaaaaa acatccattt gaacaaagta gaaattgtcg ttttggatga
      541 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      601 gcaccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gaacaacaca tcatcgccgt cgataccttc caaaagctca acctgctcga
      781 acggctgatt gtcgatctgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcagcctgcg
      961 cgtcctcgtc gccaccgaca tcgccgcgcg cgggctggac attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa accgaaatgc gcgaaccgag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaaa
     1321 tgcggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaaccg gactgacgca
     1501 tcgggagcaa ccgcccgcac cggataaatt tctgccgcaa acagtttcag acggcatttg
     1561 ctgcctgtac aatatagtgg att
//
LOCUS       DAWHXS010000183         1556 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWHXS010000183 DAWHXS010000000
VERSION     DAWHXS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.183"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..806)
                     /locus_tag="WIE22_002015"
     CDS             complement(<1..806)
                     /locus_tag="WIE22_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ4128028.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAAVLAGVGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQAQGYQYDPETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDSSIMRL
                     MYDYSRFPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFNWNGGDC
                     VVNKGDDYRNGADFSLIRNSKYKEEMDAKKLEEILSLKVDANPDKYIKATG"
     gene            complement(790..1110)
                     /locus_tag="WIE22_002016"
     CDS             complement(790..1110)
                     /locus_tag="WIE22_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ4128029.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            complement(1229..1528)
                     /locus_tag="WIE22_002017"
     CDS             complement(1229..1528)
                     /locus_tag="WIE22_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128030.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
BASE COUNT          445 a          343 c          274 g          494 t
ORIGIN      
        1 ccggttgcct ttatgtattt gtcgggattg gcatcgactt tcaacgataa aatctcttcc
       61 agctttttgg catccatttc ttctttgtat tttgaattgc gaataaggga aaaatcagcc
      121 ccatttctgt aatcatcacc tttattgacc acacaatctc cgccattcca attaaatgtg
      181 caacgattta aaacaaaatt attccaatcc aaagaactta atttattcag ttcttcttta
      241 tgccaattcc aaaacggacg tgccagccta tacatttggc tttccatcaa ttctttgact
      301 tcggggaatc tgctgtaatc gtacataagg cgcataatcg aactgtcaac gccgtagcag
      361 ccataggttc tattaatacg tctttcgtct tcgtaccaaa ggcaattact atattcgtag
      421 ccttttacaa atttgtcggt ttcggggtcg tattggtagc cttgtgcctg tatgtcttct
      481 ttgaaagttt cgtatacgtc gtgggctaaa agggcggttc cgacataggg aaccgccctt
      541 gtgctgaatt tcgcgcctaa gcgggcaagt ttgccgactc ctgccaagac ggcggcgcgg
      601 gatacgctgg cggttatctt tgcgttgatt cgggcttttg cgcccgtggg gatatgttcg
      661 acatttgccg cttctgtgaa tttagaaaat tcattattga tttttctata tcctgtagat
      721 tcaaaaaact ttgatttaga tggcctaaat tttattattc gatcatctat tcttgctggt
      781 tctgcaaaac taagagaaca acacattaaa atcggtgttg ctatcagaaa attccgagta
      841 aataaattca tgcgaaattt ttccattttc ttctgacttc ctaataaaaa tattagactc
      901 atcagaaaaa taaattttcc aaatattatg cgttaccctc ttatttaaaa aataagagaa
      961 acattctatt acgtcgtatt cttttacatt tcttacaaac tcttcaaatt ccttagacgc
     1021 aattaatgcc atcgactgcc caaaatactt gctggacggc tgatatttat aaagtgccaa
     1081 ctgcgcctgc gtgataaacg gcttgttcat gtttctgtct ttcaaaggtt gttttgaaag
     1141 cctgatttta aaacacgtca tataaatatc aaagcgacag acaaagccag gaaaaatccg
     1201 agcaaaaacc aaaaatcgac aaacatcatc acgcccctac tttgcctatg tcttttaaga
     1261 aattaatcag cagcctgaag ccgtaaataa cgacaaacag aattaaaacc atagacccga
     1321 gataagctcc ggatttaact tgttcgtaat tcgaacattt cggataagac agcgtgaccg
     1381 gctttccgtt caaaatccat ttatcgccca ccctttccgg cctgatgatt tttccgtcct
     1441 gggtaacagt aggaggaagg gacgacaata aatagtcgtc tgcctgcaat cttgtatcaa
     1501 aacaatttat gccgacacga tagcccattt atacgcccct ttttcttcac tctgtt
//
LOCUS       DAWHXS010000184         1523 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWHXS010000184 DAWHXS010000000
VERSION     DAWHXS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.184"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..194
                     /locus_tag="WIE22_002018"
     CDS             <1..194
                     /locus_tag="WIE22_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ4128031.1"
                     /translation="NNRVNQAEEDNYSTSVGSCINGFPVRYYPFIREKQQLTQQELVG
                     YHQQVEQLVQSFVNNSSKK"
     gene            211..>1523
                     /locus_tag="WIE22_002019"
     CDS             211..>1523
                     /locus_tag="WIE22_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein frpC"
                     /protein_id="HEZ4128032.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINENNGR
                     LPNTSEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSEWDNRKQLISALDKGFDGYFKEKPFTFLQSVIMDVTKLSVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGALFDHRNQGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVW
                     QDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQG"
BASE COUNT          471 a          281 c          367 g          404 t
ORIGIN      
        1 ggaataatcg agtaaaccag gctgaagaag ataattatag cactagcgta ggttcctgta
       61 ttaacggttt cccggtacgg tattacccgt ttattcggga aaagcagcag ctcacacagc
      121 aggagttggt aggttatcac caacaagtag agcaattggt acagagtttt gtaaacaatt
      181 caagtaaaaa ataatttaaa ggatcttatt atgaatgagg gtgaagttgt tttaacacca
      241 gaacaaatcc aaaccttgcg tggttatgct ttccgtggcg atacctatgg cggttggcgt
      301 tatttggcta atttgggtga ccgttatgcg gatgatgctg ctgcaattgt cggtaaggat
      361 acaaacttaa atggtttgaa tttatggatg aaaaaagggg tggaaaacct atgggatgat
      421 acggtcggta aaaagacccg tttagagaaa tttgatcggg ttgcactgca acatttcagg
      481 caatatgcgc gtctaattaa tgaaaataat ggtagattac ctaacactag tgaaattgag
      541 agaagttact ataaagccgt taccgataat ggcgtttctt ccagtgcagc tattgattta
      601 gttattaatc gttcacttcc ggatatggcg gatggttatt gggcattagg tttggggata
      661 gaagccgaac gtatccacaa tgagcaagca gtaaataatc cgaacggtag cgaatgggat
      721 aatagaaagc agttaatatc tgctttagat aaaggatttg atggatattt taaagagaag
      781 ccttttactt ttttacaatc tgtgataatg gatgtaacaa agttaagtgt tgaatataca
      841 atagatggtt ggcaaaaaat tggaggttgg ggtaatggga taatcaatga tttatataaa
      901 agtgttgcaa aaagagagtg gactggaata tttgagatcg ttaataataa catcaagcaa
      961 tttagagatc tgttcccaaa tccggaaggc tggatcgatg atggtcacca atgtttcgct
     1021 ccttgggtta aagaaactaa aaaacgcaat ggcaaatatc atgtctacga ccccctcgcc
     1081 ctagatctag acggcgacgg tatagaaacc gttgccacca aaggctttgc aggtgcattg
     1141 ttcgaccacc gcaatcaagg catccgcacc gccaccggtt gggtttctgc cgatgacggt
     1201 ttactcgtcc gcgatttgaa cggcaacggc atcatcgaca acggtgcgga actcttcggc
     1261 gacaacacca aactggcaga cggctctttt gccaaacacg gctatgcagc tttggccgaa
     1321 ttggattcaa acggcgacaa catcatcaac gcggcagacg ccgcattcca aaccctgcgt
     1381 gtatggcagg atctcaacca ggacggcatt tcccaagcta atgaattgcg tacccttgaa
     1441 gaattgggta tccaatcttt ggatctcgcc tataaagatg taaataaaaa tctcggtaac
     1501 ggtaacactt tggctcagca agg
//
LOCUS       DAWHXS010000185         1478 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWHXS010000185 DAWHXS010000000
VERSION     DAWHXS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.185"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..900
                     /locus_tag="WIE22_002020"
     CDS             <1..900
                     /locus_tag="WIE22_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HEZ4128033.1"
                     /translation="NLGLVIVDEQHRFGVAQRLALKNKGREVHQLMMSATPIPRTLAM
                     SFFADLDVSVIDELPPGRTPIKTRLVNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEES
                     ETLQLQTAAETLARLQTALPELNIGLVHGRMKAAEKAEVMARFSSGGLNVLVATTVIE
                     VGVDVPNAALMVIEHAERMGLAQLHQLRGRVGRGAAESVCVLLFAEPLGELAKARLKV
                     IYEHTDGFEIARQDLNIRGPGEFLGARQSGVPMLRFAKLEEDLHLLEQAREIAPILIE
                     QNPEIVEAHLARWLSGREGYLGV"
BASE COUNT          361 a          376 c          411 g          330 t
ORIGIN      
        1 aatctaggct tggtgattgt ggacgaacaa caccgctttg gtgttgccca acgcctcgcc
       61 ctcaaaaaca aagggcgcga agtccatcag ctgatgatgt ccgccacgcc catcccgcgc
      121 acgcttgcga tgagtttttt cgccgatttg gacgtgtccg tcatcgacga attgccgccc
      181 gggcgcacac cgattaaaac gcgcctcgtc aacaacgtcc gccgcgctga agtcgaaggc
      241 tttgtcctcg gtacttgccg aaaagggcgg caggcgtatt gggtttgccc gctgattgaa
      301 gaaagcgaaa ccctgcaact gcaaaccgcc gccgaaaccc tcgcccggct tcagacggca
      361 ttgcccgaac tcaatatcgg actggtacac gggcgcatga aggccgccga aaaggccgaa
      421 gtgatggcgc ggttttcttc gggggggctg aacgtcttgg tcgccaccac cgtcatcgaa
      481 gtcggcgtag atgtgcccaa tgccgccctg atggtcatcg aacacgccga gcgcatgggc
      541 ttggcgcagc tgcaccaatt acgcggtcgg gtcgggcgcg gcgcggcaga aagcgtgtgc
      601 gtcctcctgt ttgccgaacc cttgggcgaa ctcgccaaag cgcggctgaa agtcatctac
      661 gaacacaccg acggctttga aatcgcccgc caagacctca acatccgcgg ccccggcgaa
      721 tttctcggtg cgcgtcaaag cggcgtgcct atgctgcgtt tcgccaagct cgaagaagac
      781 ttacaccttt tggaacaagc gcgcgaaatc gccccgatac tgattgagca aaaccccgaa
      841 atcgtcgaag cgcatttggc aaggtggctt tccggcaggg aagggtattt gggcgtgtga
      901 gcaaaatgcc gtctgaaata ttagaaaaaa aagtgtaacg gaaatctgat tttttatagt
      961 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata gtacggaacc
     1021 gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggtaac
     1081 gccgtactgg tttttgttaa tccactatat tatttttgac acattttcat gatttcaaat
     1141 cccgttattc ccaagtggac ggaaatccgg taatgggaag ccgcagagat ttatcgggaa
     1201 acggcaagcc ctccgccgtc attcccgcgc aggcgggaat ctagaaatga aaaaccacgg
     1261 tgttatcggc aatgactgaa accggagaaa tgacgaaatt gaagcgtgcg gaaatgtgtc
     1321 ggaaatcgct gaagccggat aaactagatt cccgcttttg cgggaatgac gggattttag
     1381 gtttttggtt tgttttcgcg gcaatgatgg gattttaggt ttttggtttg ttttcacggc
     1441 aatgatggga ttttaaattg caggtattta ccgggaaa
//
LOCUS       DAWHXS010000186         1472 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWHXS010000186 DAWHXS010000000
VERSION     DAWHXS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.186"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            111..534
                     /locus_tag="WIE22_002021"
                     /pseudo
     CDS             111..534
                     /locus_tag="WIE22_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            640..1203
                     /gene="pgsA"
                     /locus_tag="WIE22_002022"
     CDS             640..1203
                     /gene="pgsA"
                     /locus_tag="WIE22_002022"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ4128034.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1283..1358
                     /locus_tag="WIE22_002023"
     tRNA            1283..1358
                     /locus_tag="WIE22_002023"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1316..1318,aa:Gly,seq:gcc)
     gene            1384..1459
                     /locus_tag="WIE22_002024"
     tRNA            1384..1459
                     /locus_tag="WIE22_002024"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Gly,seq:gcc)
BASE COUNT          354 a          348 c          360 g          410 t
ORIGIN      
        1 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgctgtactg gtttttgtta
       61 atccactata ttatcgctgg gtatggtttg ccgccataag ctgtctgctg ttgtccgccg
      121 tttttattgc accttacctg acggcatttc acgaacaaga aaaaacatta gaatatgcgg
      181 aattgaccgt taccgccccc aaccgcagcg gacgggcaat caaactggat gcagacgggc
      241 agcattaccg gctttcctgc tatgggttcg acagtttgtg cacaggcggc aatatcggca
      301 gaaccatcag ggcggagcag gttagagccg ttttaagcga aaccgtcggc aaaggttttt
      361 taaacggcgt tctgttggaa taccgcaaca gtggcagtgt ctataagcaa taaagatttt
      421 gcccgcacgg aagaccgcct tgtcgaagtg ttggcacaac ctgccgtttt cagcctgaaa
      481 ctgggtattt tgcttttatt gcccgccatt ttcctgcgtt tgaaaagaat gtgaaaacag
      541 attggcgggg cgggggaatg cccatgcagc aattttacgt tttgggttat gaggataacc
      601 gttataatca caactttaca gtctgcacag agaaaatcga tgccttggaa tctccccatc
      661 ttcctcacat ggttgagggt cttgctcatt cctgtcatga cagccctttt ttacctgcct
      721 tttccgtggt tcgcggagga aacgattaat ctcaccgccg ccgtcatttt tgccgttgcc
      781 gccttaaccg actggtttga cggatttttg gcaagactgt ggaaacagac ttccgatttc
      841 ggcgccttcc tcgatcccgt cgccgacaag ctgatggttg ccgtatcgct acttttactg
      901 gtcaaactcg accggaccta tgttttgttt gccatgatca ttatcggcag ggaaattacc
      961 atttccgcat tgcgcgaatg gatggcgcaa atgggcaaaa ggaacagcgt tgccgtcgcc
     1021 accgtcggta agttcaaaac cgccgcgcaa atgctggcaa tcttcctgct gctgctgaat
     1081 attcccgatt tttacggatt taatcttgtt gttatcggca atgtattgat gtttatcgca
     1141 tctttgctga cagtctggtc gatgctgtat tatctgaaaa tggcgtggaa agaaatcgcc
     1201 tgaaaaaaac ataaaaatag cttgacggca aaaacagaat ccataataat tgcgtctctt
     1261 cgatgttgga gaatgaaatc gggcgggaat agctcagttg gtagagcgca accttgccaa
     1321 ggttgaggtc gcgagttcga gactcgtttc ccgctccaaa aatttcattt tcccacaatc
     1381 attgcgggaa tagctcagtt ggtagagcgc aaccttgcca aggttgaggt cgcgagttcg
     1441 agactcgttt cccgctccaa gttttatttc aa
//
LOCUS       DAWHXS010000187         1445 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWHXS010000187 DAWHXS010000000
VERSION     DAWHXS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.187"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            27..1364
                     /locus_tag="WIE22_002025"
     CDS             27..1364
                     /locus_tag="WIE22_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ4128035.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          264 a          340 c          389 g          452 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtaaaaatg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggt caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcggtgt
      901 ttatgaaaat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat cgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttt attgtcggca
     1081 tcccgtccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactacctt atttctgcag tcatcatgcc tgtcggcgca ttgagcgttg
     1201 ccgtatttac cgcttggatt caggacaggc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ttgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc atcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaa
//
LOCUS       DAWHXS010000188         1394 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWHXS010000188 DAWHXS010000000
VERSION     DAWHXS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.188"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..>1394)
                     /locus_tag="WIE22_002026"
     CDS             complement(<1..>1394)
                     /locus_tag="WIE22_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ4128036.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFL"
BASE COUNT          248 a          299 c          460 g          387 t
ORIGIN      
        1 agaaaattgc cgtctgaagg ctggcggatt ttttgaaaaa gccacggggt tgctgcatct
       61 ttttgagttt gtttgtcgct gctggcttcg cgcatggttt tgactgcctg aatacgggct
      121 tgaacttggg gcatctgttt caggtcgtgg ttgcgtttaa cgtcggatac gccgtgaaac
      181 cacaggcacc aacgggtttt gccgttgata aattcctctg cgccgacaaa gggacggatg
      241 tattgttccg ccaagggttc ggcggcaatc agggcttctt tttcatcggt tgaaaggata
      301 aggttgccgc cttcggtcgg tttgcttccg ttgaccatgt caggttcgca atgtacggga
      361 cggctgcgct tggcaataat cacatcgggc gcgtcaatca gatacggatt gatgttggcc
      421 gcggcgtgtt ttacgggttc gcctttgatg tcgggatagt cgtagagggt tttttcagac
      481 ggcatttgcg gcttttggcg gaagccgacg atgatgcagt ggacggcggc tttgcctgcg
      541 gcttggctcg tccattgaaa ggtgcggtgg gcaaagtgga tttcgatgtc ttgcttcaaa
      601 aggctgcccc aaaggatttc gacctgctgg ccttggcaga tggaattggt ggaaacaaat
      661 gccgtctgaa cttggggatg ctgcgccatg ataccggcgg ctttgacata ccagttgcag
      721 acgtaatcca acaggccgta gcctttgata tggccgcaga tgctttcgag gtcgtttttc
      781 tgctctttgg tttggtaggt cgagccgata aagggcggat tgccgaagat gtagtccgcc
      841 gcctgccaag gtgtgcggag gctgttggcg tggatgattt cggcggcgtc ttcgagcggc
      901 agcgtatggc aggcgacttc gccgtcgaag cggatttgcg tgcggatgtt gcattggtgg
      961 tttttgagcc acatggcggt gcgggcgatg aggacggcaa attcgtctat ttcgatgccg
     1021 tgaaactgtt tcagacggca ttggacggac gggctgtcga ataagccgcc tgttttgtct
     1081 ttgagcgctt cggcgatgat gtcgtcttcg agggcgcgga tacggtcgta ggcgacgata
     1141 aggaagttgc cgcagccgca ggccgggtcg aggaattgca ggttttggat tttttggtag
     1201 aaggcggcga gttttttgtc tttgtcgcgt ttgagggctt tgacggcttc aaattcggcg
     1261 cgcaggtttt ctaaaaaaag gccgttgatg actttgtcga tgttggcggt ttcggtgtag
     1321 tgtgcgcccg cttcgcgccg ctcttgtgcg tccatgactg attggaagag cgtgccgaag
     1381 atttcgggac tgat
//
LOCUS       DAWHXS010000189         1368 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWHXS010000189 DAWHXS010000000
VERSION     DAWHXS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.189"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            93..189
                     /gene="ffs"
                     /locus_tag="WIE22_002027"
     ncRNA           93..189
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WIE22_002027"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            357..>1368
                     /gene="dld"
                     /locus_tag="WIE22_002028"
     CDS             357..>1368
                     /gene="dld"
                     /locus_tag="WIE22_002028"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ4128037.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDL"
BASE COUNT          343 a          402 c          351 g          272 t
ORIGIN      
        1 tcaaatataa attttaggtg gtgtttccgg cgtatgaccg tatcaaaacg gttgagtttt
       61 tcagaaaacc cgttataatg ccgtttctct gaggcgggtc tccccgcatg gcaaatcgga
      121 acaccgggtc aggggcggaa gccagcagcc cactccgatg cgccagtgcc gggggtttgg
      181 cccgccgccc tatttgaaac gccggagctt cgatgttccg gcgttcccgt atcgcaccga
      241 tgccgtctga aagccgttca gacggcattt cctttacttt gccataacgc ataactacaa
      301 tgggaaaaca atttcaatgt gcatatgcac ctgccgacaa ttttaaggaa actgccatga
      361 gcgcatcaca attactgtca agactgaccc aaaccgtagg cgaaaaatac atcatcaccg
      421 accccgcgaa aaccgaacaa taccgccaag gctaccgctt cggcgagggt aaggcgttgg
      481 cggtggttcg ccccggaagc attctggaaa tgtggaaaat tctgcaggcc tgcgtcgaag
      541 cggacgtgat tgtgattacg caggcggcga ataccggttt gacgggcggc tcgacccccg
      601 acggcaacga ttacgaccgc gacatcgtga ttgtgaacac catgcggatg aacatcatcc
      661 aaaccatcaa caacaacgaa caagtcgtct gcctgcccgg ttcgaccctg aaccagcttg
      721 aattgctgct gaaacctttg gggcgcgaac cgcattcggt catcggctca tcctgtatcg
      781 gtgcgtccgt cttgggcggc gtgtgcaaca actccggcgg cgcgttggta cagcgcggcc
      841 cggcctacac cgaaatggcg ttgttcgccc aaatcaacga agaaggcagg ttggaactgg
      901 tcaaccactt gggcatagac ttgggcgaca cgcccgaaga aatcctgacc aacctgcaag
      961 gtcatcatta tcaaaacaaa gacatcaaac aagacgcggg caaagggcac gaccacgcct
     1021 attgcgaaca cgtccgccaa gtggacgaac cgaccgccgc gcgtttcaat gccgaccccg
     1081 cccgccatta cgaagcctcg ggctgcgcgg gcaaactgat ggtttttgcc gtccgtttgg
     1141 acaccttccc gcaagaaaaa caaaccgccg tgttctacat cggcacgaac gacatcaacg
     1201 agctgaccga catccgccgc gccgccttgg gcgaatttga aagcctgcct gtttccggcg
     1261 aatacatcca ccgccacgct ttcgacattg ccgacgtgta cggcaaagac acgttttacg
     1321 tcatcaaaaa attcggtacg caccaactgc cgaaattatt tgacttga
//
LOCUS       DAWHXS010000190         1239 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWHXS010000190 DAWHXS010000000
VERSION     DAWHXS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.190"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(333..743)
                     /locus_tag="WIE22_002029"
     CDS             complement(333..743)
                     /locus_tag="WIE22_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128038.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(747..1214)
                     /locus_tag="WIE22_002030"
     CDS             complement(747..1214)
                     /locus_tag="WIE22_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128039.1"
                     /translation="MQTLDGEMAGGNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTH
                     TTYNGGSAITDGKYITDPLSMVRHKPDASSLDKIDSKSLFKSDVDAEKATLDAARYAD
                     KHNLWDSQNKAKVPTNDVVGYTDGKPTHIINIYRRKPNKDGFRPVHGTPGKER"
BASE COUNT          369 a          227 c          201 g          442 t
ORIGIN      
        1 ttgtatccga caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttgaaaaac
       61 gtcggattcg agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa
      121 tccatgtttc gccattttcc tttatatata cctaatgcag cacgggttac gatttcgttt
      181 aagtcataaa tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa
      241 acccaacaaa aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcaatg
      301 aattaggttt taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgc
      361 actgaacaaa caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa
      421 atgtttacat ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca
      481 tgaggatatt tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt
      541 aattttgcac acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca
      601 attccttcat cccaatcaaa agtaaaatcc gttcctttta tgataaattt agcacaagat
      661 attaaatcta acttaaccca agtggattta tacagactca atgctgatga agttataata
      721 ttatctaaat tataaatttt cataaatcaa cgttccttac ctggtgttcc atgcacaggt
      781 ctaaacccat ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg
      841 tctgtatagc caactacgtc gttcgttgga acttttgcct tattttgact atcccataaa
      901 ttatgtttat ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat
      961 ttaaataaac ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtacc
     1021 atactgagag ggtcagtaat atatttacca tctgtaatag ctgatccacc attataggta
     1081 gtatgcgtat aatttttttg tggaacgaga ctattttgca aagattgttg tgtagaaacg
     1141 ccagctttgc cattaggagt agcttgcttc gctggcttat tcccaccggc catttcccca
     1201 tctaaagtct gcataggtcg ggcagcaggt gttttatag
//
LOCUS       DAWHXS010000191         1222 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWHXS010000191 DAWHXS010000000
VERSION     DAWHXS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.191"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(146..502)
                     /locus_tag="WIE22_002031"
                     /pseudo
     CDS             complement(146..502)
                     /locus_tag="WIE22_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            517..1170
                     /locus_tag="WIE22_002032"
     CDS             517..1170
                     /locus_tag="WIE22_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4128040.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEDPVEPDESDFGGWRKGRCGRGAAGKVVVFDILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIYYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTSSRQLKILQDWCGI"
BASE COUNT          382 a          262 c          258 g          320 t
ORIGIN      
        1 gcctctaaaa aataatcgct tgttttgaca ccgatacact catatagtgg attaacaaaa
       61 atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggcaaggc gaggcaacgc
      121 tgtactggtt taaatttaat ccactataca aatacagaaa ctcaagaaaa taaccttgtg
      181 tattaaccat ctcaagcaat tcagaaaaat caagaaattt tctgaccgta aacaaacgtt
      241 tccctaaaaa aacgatgtct tcaaaaatat cgaccaaata atcatcttcg ccgttttcat
      301 ttttatgggt taaaacaaca cgggaaaaat ctgttttcag atgcttgccc gcttgattgt
      361 tcggattatt gtccggaacg acaaaaccgt cctcaaaatc aaagtagacg ttgcgtcctt
      421 ctacctttat ctctgtgcaa taacaatcat gcagagaaat accatccgaa aaatttaggg
      481 gctgtactag attagcagat atgttaccct cgaaatatga agataacgca ctgcaaatta
      541 aagaaaaaag tacagaaaga actgctccgt ttttttgtgc tggaagttac cgcccgttct
      601 gccgccgata ttttgggtat ccatcccaat tcggcagcac tgttctaccg taaaatccgc
      661 acggttatca accatcattt ggccctggct gccgatgagg tttttgagga tcctgtcgag
      721 ccggacgaaa gcgatttcgg cgggtggcgt aaaggcagat gtggtcgtgg tgcggcagga
      781 aaagtggttg tcttcgacat tctgaaacgc aacggacggg tctataccgt tgtcgtaaac
      841 aatgccaagt ctgaaacgtt actccctgtc atcaaaaaga aaatcatgcc ggacagtatt
      901 gtttataccg atagtctgag cagctgcgac aagttggacg tgagcggttt catttattac
      961 cgcatcaacc attccaagga atttgcagac cgtcagaacc acattaacgg cattgagaat
     1021 ttttggaatc aggcaaaacg tgtcttgcga aaatacaatg gaatcgatcg caaatctttc
     1081 ccgctgttct tgaaagaatg cgaatttcga tttaacttcg gcacatcgtc ccggcagcta
     1141 aaaatcctac aggattggtg tgggatttag ggctaatcta gggcgtgtcc tcaatttaac
     1201 ttagcatgaa taatagagca ag
//
LOCUS       DAWHXS010000192         1221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWHXS010000192 DAWHXS010000000
VERSION     DAWHXS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.192"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(356..766)
                     /locus_tag="WIE22_002033"
     CDS             complement(356..766)
                     /locus_tag="WIE22_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4128041.1"
                     /translation="MDSRLRGNDEAILLVEGQKSAVTEYYLNHGIWPSDNSAAGVASS
                     ADIKGKYVQKVEVNNGVITATMASSNVNNEIKGKKLSLWAKRQDGSVKWFCGQPVTRD
                     APNASADAVNKVTGNEIDTKHLPSTAPTRKSTPN"
     gene            complement(886..>1221)
                     /locus_tag="WIE22_002034"
     CDS             complement(886..>1221)
                     /locus_tag="WIE22_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4128042.1"
                     /translation="GVNKEIQGKKLSLWAKRQNGSVKWFCGQPVTRAANNTAKDAVTA
                     ANDNSNNGINTKHLPSTCRDAASAICTRTPRADFKHFQKISRYRVLPESRHMAEKLRH
                     SRKSGNLVR"
BASE COUNT          245 a          257 c          338 g          381 t
ORIGIN      
        1 aaagaatggc ttcgtcattc ccgcgcaggc gggaatccag gtctgtcggt gcagaaactt
       61 atcgggaaaa cggtttcttg agattttacg ttctggattc ccactttcgt gggaatgacg
      121 ggatttaagt ttcaaaattt attctaaata actgaaactc aacgaactag attcccgctt
      181 ttgcgggaat gccgaatttt aggtttctta ttttggtttt ctgtccttgt gggaatgatg
      241 aaattttaag ttttaagaat ttatcggaaa aaacagaaac cgctccgccg tcattcccgc
      301 gcaggcggga atctagcctc gtcggtgcgg aaacttatcg ggtaaaaagg tttctctagt
      361 ttggtgtcga ttttcttgtc ggtgctgttg acggcaggtg cttggtgtcg atttcgttgc
      421 cggtgacttt gttaacggcg tcggcgctgg cgttgggggc gtcgcgcgta accggctgtc
      481 cgcagaacca ttttaccgaa ccgtcttgac gcttggccca cagggagagt tttttgcctt
      541 tgatttcatt gtttacgttg cttgaagcca ttgtggcggt aatgacgccg ttgttgactt
      601 caactttctg aacatatttg cctttgatgt cggcggagga tgccacgccg gcggcactat
      661 tgtcggatgg ccatatgccg tgattcaggt aatactctgt gacggctgat ttttgacctt
      721 cgaccaaaag gatagcttcg tcattcccgc gcaggcggga atccagcctt gtcggtacgg
      781 aaacttatcg ggtaaaacgg tttctttaga ttttgcgttc tggattccca ctttcgtggg
      841 aatgacggga ttaaagtttc aaaatttatt ctaaataact gaaactcaac gaactagatt
      901 cccgcttttg cgggaatgac gaagtttttc ggccatgtgc cgtgattcgg gcaatactcg
      961 gtaacggctg attttttgaa aatgtttgaa atcggcgcgt ggtgttcttg tgcaaatggc
     1021 agatgccgca tcgcggcagg ttgacggcag gtgtttggtg ttgattccgt tgttgctgtt
     1081 gtcgttggcg gcggtgacgg cgtctttggc ggtgttgttg gcggcgcgcg taaccggctg
     1141 tccgcagaac cattttaccg aaccgttttg acgcttggcc cacagggaga gttttttgcc
     1201 ttggatttct ttgtttacgc c
//
LOCUS       DAWHXS010000193         1198 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWHXS010000193 DAWHXS010000000
VERSION     DAWHXS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.193"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            28..564
                     /locus_tag="WIE22_002035"
     CDS             28..564
                     /locus_tag="WIE22_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ4128043.1"
                     /translation="MLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDN
                     LKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYD
                     TPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRLHAHVA
                     ALCSDDEIYRTTMAAYTM"
     gene            584..>1198
                     /gene="pglH"
                     /locus_tag="WIE22_002036"
     CDS             584..>1198
                     /gene="pglH"
                     /locus_tag="WIE22_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ4128044.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAI"
BASE COUNT          269 a          369 c          314 g          246 t
ORIGIN      
        1 ttctggaaaa ccgtcaaagg ctgcgtcatg ctcggcacgc tgtcgcgtct ggacaccgtc
       61 cgcgccgtgc atctgatgct ggacattttc aaaaaaatgg tcgaccgcga tatgcccgta
      121 cgcctgaacg tggcgggcat aggcgaagaa atggacaatt tgaaagcgca ggcggaacgt
      181 ctgggcatag acggcaaagt taccttcctc ggcggcgtgc gcgatttgac cgcctacttc
      241 aaagacgtgg atattttggt gaacacgccc cactgcatcg gcgaccacgg cgcaggtgtg
      301 ggcaacaaca tcttagaagc cggactttac gacacacccg tcgttaccta cgatatggcg
      361 ggcatttccg aaatggtcgt caacggggaa acgggttact gcatcccctt cggcgacgaa
      421 gaagccttta tcgccgccgt agacacgctc gtcagacaac cgctgttgcg cgaagcaatg
      481 ggcaaaaggc tgcacgcaca cgtcgccgca ctctgttccg acgacgaaat ctaccgtacc
      541 accatggcgg cctataccat gtaatgccgt ctgaaagccg ccgatgaaca tcaccatagt
      601 cgccccttat tgctcgctgc cgtccgaacc gtatttcaac cgcttttggt atctggcaga
      661 acgattgtcg caatcgcacg acgtgttgct gattaccagc cgtttccgcc actacgacaa
      721 atcattcaga cggcatgaag atgctgccgc cacttcaaac ggcaggctgc gcgtcaagct
      781 gctggacgaa cctggctacc gaaaaaacgt ctccctcgcc cgcgtcgcca gccaccgcgt
      841 attcgtccgc aatttggcac gctggctgca cagcccgcag gcggcggaac aggacatcgt
      901 ctattctgcc tatccgctga tggcgaccaa cctgctgttg ggcaaacaca aagcgcgttt
      961 gggctacaaa ctgattgtcg atgtgcagga cgtatggccg gagtctttct cctcggtcgt
     1021 accatttttg aaaaaagtac cgcacaagct gctgcccttt gcttcacgcg ccaaccgtgc
     1081 ctaccgctgc gccgacgcgc tggttgccgt atcgcagact tatctcgacc gcgccaaaga
     1141 agccaatccg aacgtacccg gcgaagccgt ctatatcggt acggattttg ccgccatc
//
LOCUS       DAWHXS010000194         1123 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWHXS010000194 DAWHXS010000000
VERSION     DAWHXS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.194"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(231..1085)
                     /locus_tag="WIE22_002037"
     CDS             complement(231..1085)
                     /locus_tag="WIE22_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ4128045.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIAAFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          310 a          337 c          294 g          182 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cgggaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgatg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caaggcggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacgttggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagccgca ccccaaaccc aaatacagca gcgacaatac catcccgacg
      481 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagtgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacaaa cgccgcaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cggcaattag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tag
//
LOCUS       DAWHXS010000195         1120 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWHXS010000195 DAWHXS010000000
VERSION     DAWHXS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.195"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            151..>1120
                     /locus_tag="WIE22_002038"
     CDS             151..>1120
                     /locus_tag="WIE22_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ4128046.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSVGVVGTPDKLAGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          271 a          322 c          287 g          240 t
ORIGIN      
        1 aaaaattagg acaaggcggc gagccgcaga cagtacaaat agtacggaac cgattcactt
       61 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg
      121 gtttttgtta atccactata tcaagtctgc gtgttggaaa aacagcactt tccgcgcttc
      181 gtcatcggcg aaagcctgct gccgcactgt atggaaatgc tggaagaagc cggatttgcc
      241 gatgccgtgc gcgccgagcc cggctttcag ttgaaaaacg gcgcggcgtt ttcgtggggc
      301 agccgttata ccgaattcga tttcaccgat aaattttcag acggccccgg cacgacttat
      361 caagtgcgcc gcgccgtgtt cgataaaatc ctgattgaag aagccgccaa acaaggcgtt
      421 gaagtacgtt tcgggcacgg cgtaaccgcg ttcgacaaca gcggcgattt tgtccgcctg
      481 aacatcgaaa ccgacaccgg caagcgctat gaactgaccg cgaaattcgt cttggacgca
      541 agcggttacg gacgcgtgct gccgcgcctg ctaaacttgg aaacgccctc gcacctgccg
      601 ccgcgccaag cgcatttcac gcacatcgac gacaacatca ccaacccgaa attcgaccgc
      661 aacaaaatcc tgattaccac ctatccgcaa caccgcgacg tgtggatttg gctgattccc
      721 ttcggcgaca accgctgttc cgtcggcgtg gtcggcacgc ccgacaaact tgccggcgaa
      781 tcggaaacag tgttgaaaaa atttgtttac gaatgcccga tgctgaacga aattttggac
      841 aaagccgttt gggaaaacga ttttccgttc cgctccatcc aaggctattc cgccaatgtg
      901 aaatcgctgc acggcaggca tttcgcgcta ttgggcaatg ccgccgagtt cctcgatccc
      961 gtgttctcgt cgggcgtaac catcgcgctg cactccgcca aacttgccgc cgatctgttg
     1021 gcaaaacaac tcaaaggcga agccgccgat tggcaaaccg aatttgccga acccttgatg
     1081 atcggcgtgg acgcgttccg cacctatagt ggattaaatt
//
LOCUS       DAWHXS010000196         1101 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWHXS010000196 DAWHXS010000000
VERSION     DAWHXS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.196"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            32..>1101
                     /gene="tuf"
                     /locus_tag="WIE22_002039"
     CDS             32..>1101
                     /gene="tuf"
                     /locus_tag="WIE22_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ4128047.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIAPIAMEEGLR"
BASE COUNT          293 a          309 c          283 g          216 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggtggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ctgcactgaa agccttggaa ggcgatgccg cttatgaaga
      541 gaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gaggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 ggaacgcggt caggtattgg ccaaaccggg tactatcact cctcacacca aattcaaagc
      901 agaagtgtac gtactgagca aagaagaggg cggccgccat actccgttct tcgccaacta
      961 ccgtccgcaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattacc gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggcctgc g
//
LOCUS       DAWHXS010000197         1067 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWHXS010000197 DAWHXS010000000
VERSION     DAWHXS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.197"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..578)
                     /locus_tag="WIE22_002040"
     CDS             complement(<1..578)
                     /locus_tag="WIE22_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ4128048.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDG"
BASE COUNT          311 a          269 c          191 g          296 t
ORIGIN      
        1 ccgtcctctc tccgccaagc cgttccaacc atttcagatt tcggcctcat gttactgtta
       61 tcgaaaaaac cgttatcgtg atccaaaagt gcctgttccg gcgtgtactc cccatcaaaa
      121 aaatcaagtg ccaaatctac ccgcgttatc ctcggcctca atgaatcttc caaaaactgc
      181 ttaagcctca attcccaacc tggatttgca atgttgcaac ctacaccttt caattcgatt
      241 aaaaccgtat ttcgctgacc tccgtaatgg acttcgccgt agtcaacttc ttccgatccc
      301 aacctaaaca tcgaatcgta aaatttattg cccttcgatt tgcatctgct cgtgatgcca
      361 aaccctaata tttcctccaa ttttttgctt aaaacaaaca tatattcggc atcggaaact
      421 aaggggcatc cggaaacttt cagcaaggaa tcttcgtgca gtgtgaatga caaccaatct
      481 ataaaaacgc cgtcctgcct gcccctacgt tgcggaattt ctaataactt cccattgccg
      541 ttagatatga aatgggaaaa atattctgct tcactcattt tgttcagtac ctttagggat
      601 ttgttttatt tcgctccccc cctgttagtc aggggggggg ctttcagccg tttcccgtct
      661 gccgcgctaa agcgcgtcca acggtcaacg accgaaagcc caatcctgac aaactgttaa
      721 agatcaagaa gaaagaccac aaccgtctgt tgtgataatt accggaaaat tcgagccaac
      781 cgaatctata taatcgaacg cctgataaag ctttgaaaaa ttttcttgtt cagcgagttt
      841 atgcggttca ccatgcctga actgatagaa acataaaacg caataatctg attttttaaa
      901 tattctccaa taggaacaag aaaatattac atttgctact gacataaaaa agcccctttc
      961 acttggctgt caaaggggaa tgttaagaaa agtaatgcgc ccctttgata gagcgcatca
     1021 tataaggcgg gaatctagcc cttggggcag cggcaatatt tataaag
//
LOCUS       DAWHXS010000198         1065 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWHXS010000198 DAWHXS010000000
VERSION     DAWHXS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.198"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            938..1050
                     /locus_tag="WIE22_002041"
                     /pseudo
     CDS             938..1050
                     /locus_tag="WIE22_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          278 a          255 c          273 g          259 t
ORIGIN      
        1 atttagggct aatctagtac agccccaaaa tttaaatcag gacaaagcga cgaagccgca
       61 gacagtacaa atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc
      121 tctttgagct agggcgaacc aacgctgtac tggtttagat ttaattcact atacctgcgt
      181 aatggtatcg ggtactcatc atcaacctgc cttgcattcg gttgtgttgt ccggcaactg
      241 tccggttgtg tcgatgggtt gccctgccgc tccctgtgct gcgcaacggt tgtgtgcctt
      301 gggtgggcgc gggtggcggc cgggcggttt gttgctatga tacgggagtt ccgatataca
      361 agggtgggct tcagcccacc aatcaacgac cgaaaaagaa aaaacgttgg gctgaagccc
      421 actctacgca attacgcaac tgttatcgtg gggatgacgg gattttaggt ttttgttttt
      481 ctgtttttgc gggaaggatg ggttttaggt tgcggtcatt tatcggaaaa gcagaaatcg
      541 ctccgccgtc attcccgcga aagcgggaat ctgaacacgt ccgtagggaa acctatatcc
      601 cgtcattccc acgaaagtgg gaatctagaa cctctgaatt ttcagacaac ctttgaatat
      661 taccgccgtc ccgcgttcta gattcccgcc tgcgcgggaa tgacgattca tcagtttccc
      721 gaaacgcccg aaaaaccgaa accgagcaaa ccggattccc gcctgcgcgg gaatgacgat
      781 ttataagttt cccgaaacgc ccaaaaaaac cgaaatccga caggcaggat ttttgctttc
      841 gtggggatga cgggatttta agttacggtc atttatcgga aaagcagaaa ctgctccgcc
      901 gttattccag cgaaggcggg aatctgaaca cgtccgcacg gaaacctata gtggattaac
      961 aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca
     1021 acgccgtact ggtttaaatt taatccacta taatcctgtt gctta
//
LOCUS       DAWHXS010000199         1049 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWHXS010000199 DAWHXS010000000
VERSION     DAWHXS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.199"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(123..551)
                     /locus_tag="WIE22_002042"
     CDS             complement(123..551)
                     /locus_tag="WIE22_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ4128049.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(532..966)
                     /locus_tag="WIE22_002043"
     CDS             complement(532..966)
                     /locus_tag="WIE22_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ4128050.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          270 a          316 c          189 g          274 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtccc gaacggtaga tgtttcaccc
       61 ctgcaaacca aaaaaaagac cgtctgaata cagacggcca aagcctggtc acacatcact
      121 gcctaaaata aagctgcctg ctgtgccgca ggtcggctca tttcattgat aatcgtatat
      181 cccgttcgtg aagagatgcc gtatttaggg catagcttcg tcatcgccat aagcccgctc
      241 tccttatcaa tatcgcgcaa tttgacaaac tcctgataaa acctatggtt tctcaactgt
      301 attaacgcct tgccgcaccg tgggacatac aattcctcgc caccatatac ctgcaacagc
      361 tcatgtgttt tcacttcgcc gatggcttcg accaaaattg ccaaacgctc ggtgtccacc
      421 ttgcccttac caaatttaaa ccgcgccccg ccaatcgcct tgaccagctg ttccgtcgct
      481 gccagtccga tgacatccac aatgtccaac acggtatccg gcaataaatg ttcaactttt
      541 tcgaacccca tcatccccac ccgctttttc cgttttcctg ttttccgcaa tctgcaacgc
      601 agcaaccagt ttatgtagct gcgtatcgtc taaatattcg accttatcct taccaaacat
      661 ccgccgcgcc attgcgtgtg catagttcca atgtttgccg ccgacggtca gcagggcttc
      721 gactttgtcc aacattgccg ctgatgatgt ccgacgcaga tgtggtttac cgtgtgggtt
      781 accttttgct ttaggcttaa atccgtgcga ccgcatatca gcgacaacag actcaagttc
      841 ggaaacatcc atatccgcac acgaccgctt gcccgtcacg cgctccaaca ccgcgcgata
      901 ggtaccgtcg tccaagccca gctccttttg ggcaatttta atttttgcaa tcaacgcccg
      961 gcgcatctca aacccataaa acacaatata ttgactaatt aacgcatatt ataccaataa
     1021 aatacaatat atagtattaa gccgatgtt
//
LOCUS       DAWHXS010000200         1000 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWHXS010000200 DAWHXS010000000
VERSION     DAWHXS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.200"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..861)
                     /gene="mafB"
                     /locus_tag="WIE22_002044"
     CDS             complement(<1..861)
                     /gene="mafB"
                     /locus_tag="WIE22_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ4128051.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WGNRMDDIRGIIQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATAL"
     gene            complement(901..>1000)
                     /locus_tag="WIE22_002045"
     CDS             complement(901..>1000)
                     /locus_tag="WIE22_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ4128052.1"
                     /translation="TGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
BASE COUNT          230 a          249 c          231 g          290 t
ORIGIN      
        1 taatgcggtt gcggccgcaa gttgtgcttc cggacttaaa tttcctaaat tgtgaatacc
       61 ctgtaaagtt ttccgtgctg ccgcataggt taccgggttt accgcactgt ctgtaattgc
      121 cccaactccc aatccctgaa aacccgttag aaaaggattg gctgcacctt ggatgatgcc
      181 gcgaatatca tccatacggt taccccacca atttttattg gggtcgcttt cccatatcag
      241 ttttcctgct tcatccgcac ggctgaaaaa accagaggcg gcaccggcat tttcttttag
      301 ccaacggtct gaaaatgggg cttgagggac aatattagtc tttgtttttg ttgaagttcc
      361 tttgacatag tagctgtata tatcccttgc tcctccgggg ggcggataat cgctgccttg
      421 cggtccgtca tatccatcct ccggatggat ttccgaccct gtccgatgaa gttggtaaac
      481 agtaaaaccg ccgtctacac cgccgctgaa atcagaagtg ctttttgaat catgatgatc
      541 gaacggactg tgtacttcat gtccgtgccc tgaaaaatgg gtttcatagc cgataacacc
      601 ttcaaaccct gtccgttcgt gtgtaatagg cagtatgggg ccgaccgcag ttgcatcaaa
      661 tgtttggacg gcgtaaaccc gatttttaac actgccgcga ccatcaccga acagatggta
      721 tttgccaccc ggttcgtagt gttttgcctg catatcatcg cgcaaacggg catccaaacc
      781 gttcgcatga ctaatgggga tttgcagcaa cgatattgct gctgcaaaca gcatcatgaa
      841 tttttgaata ggcaaattca ttttgttttc cttggtagca agcggttatg gtagtgtgaa
      901 tcaaggttgc ccttgtctat gttgtcgcac tacttcatcg ctgtatccat acccctcatg
      961 actgttatca gtctctacgg atggggcgga gttacccgta
//
LOCUS       DAWHXS010000201          978 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWHXS010000201 DAWHXS010000000
VERSION     DAWHXS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.201"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            220..891
                     /locus_tag="WIE22_002046"
     CDS             220..891
                     /locus_tag="WIE22_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ4128053.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          280 a          225 c          215 g          258 t
ORIGIN      
        1 acggatgaaa tcaaccgata ttcaaacaca gtcattttta gcgcattttc agcgtatcgt
       61 taatgcggaa aatttcgtga acaggttttt tgcacaggtc tccgcctcgt ttcgcgggat
      121 gggaaaccgt attagaaaac ggacgcacgc aacataaacc ccgaaagtga tgataagatg
      181 atgatttaac gtactgcttt aattatttaa ggaattatcg tgtttcccga caaatacaag
      241 ttgagtttgg aagaaaatat ttttctggca aagaaagtat tggttgccca aattcacaac
      301 ctcagccgtt ttgagaattg tcagacgacc ttgttgcaga ccgaacaaat tatccatggc
      361 aaaaatgtag cctccgcgtc actggaagac atccaaacca tcttgaacct gaaacgtgcc
      421 tatcaatatg tgatttcgca tatttcaaac ggcgaaccgg tcgatatttc actccttaaa
      481 aaaatcaaca acattgttgc caaggacgat tctttggcac ccggtgattt ccgtaccggt
      541 tcggtcggcg taacgctatt ggacggttcc cgtcatgccc caaatccggt gaaggaaatt
      601 gaagtggctc gcgtgttaca aaatatcgga ctgcaaagcg gttcgacgac ggaggcagcc
      661 gtccgtttta tgctttattg tatgcggcag caggtttttt gggacggcaa caaacgaacg
      721 gcaaccttat ttgccaacgg tctgatgatg gcggggggct gcggcatctt ggaaatctcc
      781 gaaatgcaga tgccgcaatt caatgaaaaa ctgtccgcat tctatcgctc cggcgacgat
      841 accgatattt ccaagtttgt gtatcaaaat tgtatatcgg gcatagactg agacctttgc
      901 aaaattcccc aaaatcccct aaattcccac caagacattt aggggatttc tcatgagcac
      961 cttcttccag caaaccgc
//
LOCUS       DAWHXS010000202          975 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWHXS010000202 DAWHXS010000000
VERSION     DAWHXS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.202"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..438
                     /locus_tag="WIE22_002047"
     CDS             <1..438
                     /locus_tag="WIE22_002047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ4128054.1"
                     /translation="NNTPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHP
                     DTGLMVLAMTAEARATADKKRLDKIRDTLAQAYGLQLTLQTQDWHDESGRETPAMQDK
                     RVQAEDRQKAQALLEADPAAQKILQAFGAQWQPESLELAANRL"
     gene            518..853
                     /locus_tag="WIE22_002048"
     CDS             518..853
                     /locus_tag="WIE22_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ4128055.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
BASE COUNT          288 a          313 c          231 g          143 t
ORIGIN      
        1 aacaacacgc cgtccgcccc gccgcccgag ttttccaccg aaaactgggc agccatcgtc
       61 cggcatttcg cccgcaaact cggcgcggcg caaatgccgg cgcaacactc cgcgtggacg
      121 gaataccatc ccgacaccgg cctgatggtt ttggcaatga ccgccgaagc acgcgccacc
      181 gccgacaaaa aacgcctcga caaaatccgc gacacccttg cccaagccta cgggctgcaa
      241 ctcaccctgc aaacccaaga ctggcacgac gaatccggca gagaaacccc cgcaatgcag
      301 gacaagcgcg tccaagccga agacaggcaa aaagcacaag cattgctcga agccgacccc
      361 gccgcacaaa aaatcctcca agcattcggc gcgcaatggc agcccgaatc actggaattg
      421 gcggcaaacc ggctataaac agatataatg ccgcccgaac ccttcggacg gcattgccgt
      481 ttcccttatt caatcaaaac agacaggagt attcagtatg ttcggaaaag ccggattagg
      541 cggcctgatg aaacaggcgc agcaaatgca ggaaaatatg aaaaaagcac aagccaaact
      601 tgccgaaacc gaaatcgaag gcgaagcagg caacggcttg gtcaaaatca caatgacctg
      661 cgcgcacgaa gtacgcaaaa tcgacatcag ccccgatttg attcaagaag ccgccgacga
      721 caaagaaatg ctcgaagacc tcatcctcgc cgccctcaaa tccgcccgtg acaaagccga
      781 agaaaccgca aacaaaacaa tgggtgcatt cacgcaaggt ctaccccccg gagtgggcga
      841 cttcttccgc tgatacccga ccgtcattcc cacgcaggcg ggaatctaga acgtagaatc
      901 taagaaaccg ttttactcga taaatttccg tgccgacggg tctagattcc cgccttcgcg
      961 ggaatgacgg catca
//
LOCUS       DAWHXS010000203          965 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWHXS010000203 DAWHXS010000000
VERSION     DAWHXS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.203"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>965
                     /locus_tag="WIE22_002049"
     CDS             <1..>965
                     /locus_tag="WIE22_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ4128056.1"
                     /translation="TTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDL
                     REAAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSEWGKKPPMHLSTSWTQ
                     TANGGVALTPSQVTRLKQNALVSLSDKAKAAIDAARDRIAVLDAYTGQDSSTLYYMSE
                     EDALNIVKVTNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAF
                     NHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQET
                     VALLAKTSGTQADDILQNVGFGHNKNVSLYGNDGNDTLIGGAGND"
BASE COUNT          290 a          217 c          225 g          233 t
ORIGIN      
        1 acaaccgcaa aaatggggga tttactttta gcagccgaca atctgcacag ccgcttcaaa
       61 gacaaagtgg aactcactgc cgaacaggca aaagccgcca atcttgcggg cattggtcgt
      121 ctgcgcgatt tgcgcgaagc tgccgcattg tccggtgatt tggccaatat gctgaaagct
      181 tattctgccg ccgaaactaa agaagcacag ttggcattgt tagataattt gattcacaaa
      241 tgggcggaaa ccgattcgga atggggcaaa aaaccgccga tgcatctttc aaccagttgg
      301 acgcaaacgg ctaacggggg tgtggctctt accccgtctc aagtaacgcg actaaaacag
      361 aacgctttag tttctctttc cgacaaagcc aaagcagcta tcgatgccgc ccgcgaccgc
      421 attgccgtgc ttgatgccta cacggggcag gattccagca cactctatta catgagcgaa
      481 gaagacgcgc ttaatatcgt caaagtaacc aacgatacat acgaccatct cgccaaaaac
      541 atctaccaaa acctgttgtt ccaaacccgt ttgcagccat atttgaatca aatcagtttc
      601 aaaatggaaa atgatacgtt cactttggat tttagtggtc ttgttcaagc atttaaccat
      661 gtcaaagaaa ctaatccgca aaaagctttt gtggatttgg ccgagatgct tgcatatggc
      721 gaacttcgtt cttggtatga aggtcgaaga ctaatggccg attatgtgga ggaggcaaaa
      781 aaagcaggta aatttgaaga ttaccagaaa gtgttgggtc aggagaccgt tgcattatta
      841 gctaaaacat cgggtacgca agcagatgat atcctgcaaa atgtaggctt tggtcataat
      901 aaaaatgttt ctttatatgg taatgacggc aacgacactc taatcggcgg cgccggtaat
      961 gacta
//
LOCUS       DAWHXS010000204          945 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWHXS010000204 DAWHXS010000000
VERSION     DAWHXS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.204"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..>945)
                     /locus_tag="WIE22_002050"
     CDS             complement(<1..>945)
                     /locus_tag="WIE22_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ4128057.1"
                     /translation="KVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSM
                     FRLGSEEVDYGEVHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVD
                     LALDFFDGEYTPEQALLDHDNGFFDNSNMRPKSEMIGTAWRREDGSGKTFYVGRKKNS
                     RFVRVYEKGRQLGDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNM
                     PNPERFDYRKKVANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKG
                     LEPEKYSLAGLKESLKFGFIHEQPDVDLEVELEELGIIK"
BASE COUNT          267 a          246 c          138 g          294 t
ORIGIN      
        1 tttgataatt ccgagttctt ccaattcaac ctctaaatct acatccggtt gttcgtggat
       61 aaagccgaat ttcaaagatt ccttcaatcc ggccaacgaa tatttttcag gttctagccc
      121 tttgggatac cccaaatctg ccttcagata tctgacaatt tcatcactat caaaacccat
      181 atcaaacatg aaattaatca gtttgccgac cgcgtttttt gcgtatctca atttatgctg
      241 aaaagttaaa ttagccactt ttttacggta atcgaacctt tccggattcg gcatattttt
      301 aaatttctga caaatcggga aagcgcctga aaagtaagaa ccttgattta tcagaatatc
      361 caaaggtatt tccatatctc catgattaaa ctgaatttcg aaccttaccc acttgctttc
      421 tttatcgcct agctgcctgc ctttctcata aacacgcaca aaacgagaat ttttcttgcg
      481 acctacataa aatgtcttgc cgcttccgtc ctctctccgc caagccgttc caatcatttc
      541 agacttaggc ctcatgttgc tattatcgaa aaacccgtta tcgtgatcta aaagtgcctg
      601 ttccggcgtg tactccccat caaaaaaatc gagtgccaaa tctacccgcg ttatcctagg
      661 tctcaatgaa tcttccaaaa actgcttaag cctcaattcc caaccgggat ttgcaatgtt
      721 gcaacctaca cctttcaatt cgattaaaac cgtatttcgc tgacctccgt aatggacttc
      781 gccgtagtca acttcttccg atcccaacct aaacatcgaa tcgtaaaatt tattgccctt
      841 cgatttgcat ctgctcgtga tgccaaaccc taatatttcc tccaattttt tgctcaaaac
      901 aaacatatat tcggcatcgg aaactaatgg gcatccggaa acttt
//
LOCUS       DAWHXS010000205          931 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWHXS010000205 DAWHXS010000000
VERSION     DAWHXS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.205"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..344)
                     /locus_tag="WIE22_002051"
     CDS             complement(<1..344)
                     /locus_tag="WIE22_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ4128058.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQG"
BASE COUNT          286 a          172 c          156 g          317 t
ORIGIN      
        1 ccttggtcgg tttttttgta atagacgcta atctctatca aattaatgga gtaagcatcc
       61 tgcccgggcg tgtcagaaag ccattttgtg gcattgtctt gcgtataccg tgcaagatag
      121 ccatagggga taaccatttt ggtgccctga aaatcaaatg taaccgtatg ttcaaatcct
      181 gtcattggct cgggattgtt gaaactggtt tgttctttag cctgttgcgc acttactgct
      241 ttttgatctg tcttttcatt cacaggttca catgaggtca tagtaaaaac actcccaata
      301 aacaaaataa aaagttgata aatggtagta gcatatggtc tcataatttc aagcctagaa
      361 attagttaaa gaatatatta tggtagatac taactcttta gttaaaaata atttactaac
      421 ttttactact taggtataaa ttccaatact taactaaact gagggtaaat atatttatcc
      481 aagatagaag agaaactttt ttctctgttt ccgcaagaat agttacgggt tgtgaaatat
      541 gctctatacc caagcagtag atattttcat tgttacttac cgtattgctt catagaattg
      601 gcactctacg ctttccttta aaaatcgtca ataacgatta tcattttctt taaatttatt
      661 caaaacaata ttcttataca catctcaagc ccgctgaaat aaagcaaaat ggtggttcat
      721 cgactgtaat gccaatttca aagcagcctg cactttgaca acacacaaga tatacgggaa
      781 ggacagggca tcagcatata tttcagcccg aagccgtttc attcgtttaa aaaactgctt
      841 ttttcacaat ttacctttac aaagcctata cgtatcctta caatttgttt tgctgttttt
      901 ggtatatttt tggggctgta ctacattagc a
//
LOCUS       DAWHXS010000206          848 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWHXS010000206 DAWHXS010000000
VERSION     DAWHXS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.206"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..797
                     /locus_tag="WIE22_002052"
     CDS             <1..797
                     /locus_tag="WIE22_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ4128059.1"
                     /translation="GLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKM
                     PAFFAQMTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQPADMRTAEKNRLHQVHETQV
                     GSVKQLIAHFDRLIDELDKQIDNHTHTHFDGKDQVAEQIKGIGSITTATLMAMLPELG
                     RLSHKRIAGLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFH
                     QRPLSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT          222 a          235 c          229 g          162 t
ORIGIN      
        1 gcggtctcga aatccccgcc gccaaagcca tccgccgagc aggcatagcc gtgattatcg
       61 ccaacccgcg tcagacgcat cagtttgccc aatcgcagcc gctgaccaaa accgacgcca
      121 aagatgccaa aatgcccgcc ttcttcgcac agatgacggc acagaaagaa gattcgcaaa
      181 ccatgcccta ccaaccgccc accgaagcgg aagaagtgtt ggaagccttg gttaaccgcc
      241 gcaaccaacc ggcggatatg cggactgccg agaaaaaccg tctgcatcag gttcacgaaa
      301 cgcaagtcgg aagcgtcaaa caactgattg cccattttga ccggctgatt gacgaattgg
      361 acaaacaaat cgacaaccac acccacacgc attttgacgg caaagaccaa gtggcggagc
      421 aaatcaaagg catcggttcg ataacgacgg ctacgctgat ggcgatgttg cccgaattgg
      481 ggcggctgtc gcacaaacgg atagcgggtt tggccggcat tgccccgcac ccgagggagg
      541 gcggggaaac caaattcaaa agccgctgct ttggcggaag gtctgcggtg cgtaaagcac
      601 tgtatatggc taccgtggca gcgacacgtt ttgaaccgct tattcgggat ttccaccaac
      661 gcccgctgtc cgagggtaag ccgtataagg ttgccgttac ggcatgtatg cgcaaactgc
      721 tgacgatatc gaatgcccgg atgcgtgatt attttgccga aaacgatacc gccgaaaacg
      781 gtatccggac ggcttgattt gagttttggt atttttgccc gacggggtga aaaatacagt
      841 tgcttttt
//
LOCUS       DAWHXS010000207          845 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWHXS010000207 DAWHXS010000000
VERSION     DAWHXS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.207"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..492
                     /locus_tag="WIE22_002053"
     CDS             <1..492
                     /locus_tag="WIE22_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEZ4128060.1"
                     /translation="VFCALTNDDESNIMSALLAKNLGAKRVIGIVNRSSYVDLLEGNK
                     IDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVVAHGDKKTSAIIGRRISG
                     IKWPEGCHIAAVVRAETGETIMGHHTETVIQDGDHIIFFVSRRRILNELEKLIQVKMG
                     FFG"
BASE COUNT          222 a          247 c          211 g          165 t
ORIGIN      
        1 gtattctgcg ccctgaccaa cgacgacgaa agcaacatta tgtccgccct tttggcgaaa
       61 aacctcggcg cgaagcgcgt catcggcatc gtcaaccgct caagctacgt cgatttgctc
      121 gaaggcaaca aaatcgacat cgtcgtctcc ccccacctca tcaccatcgg ctcgatactc
      181 gcccacatcc ggcgcggcga catcgttgcc gtccacccca tccggcgcgg cacggcggaa
      241 gccatcgaag tcgtcgcaca cggcgacaaa aaaacttccg ccatcatcgg caggcgcatc
      301 agcggtatca aatggccaga aggctgccac attgccgccg tcgtccgcgc cgaaaccggc
      361 gaaaccatta tgggacacca taccgaaacc gtcatccaag acggcgacca catcatcttt
      421 ttcgtctcgc gccggcgcat cctgaacgaa ctggaaaaac tcatccaggt caaaatgggc
      481 tttttcggat aaaccgcccc attccggaca tattgccgcc aagcggtatg gaagcagaaa
      541 taatggtagg tgggcttcag acggcatccg ccctccccgt cattcccgcg caggcgggaa
      601 tctagacctt gggatagcgg caatattcaa aggttatctg aaaatttaga ggttctagat
      661 tcccgttttc acgggaatga cgaagagttg cgggaatcta gaccattgga aagcggcaat
      721 attcaaaggt tagctgaagc tttagaggtt ctagattccc gtttccacgg gaatgacgaa
      781 aggtggcggg aatccagaac gtcgggcaac ggcaatattc aaaagccgtc tgaaaattta
      841 aaagt
//
LOCUS       DAWHXS010000208          832 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWHXS010000208 DAWHXS010000000
VERSION     DAWHXS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.208"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..413
                     /locus_tag="WIE22_002054"
     CDS             <1..413
                     /locus_tag="WIE22_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ4128061.1"
                     /translation="KAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIAGLVGIAPHP
                     RESGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRLPSEGKPYKVAVTAC
                     MRKLLTISNARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT          187 a          186 c          239 g          220 t
ORIGIN      
        1 gcaaagccca agtggcagaa caaatcaaag gcatcggttc gataacgacg gctacgctga
       61 tggcaatgct acccgaattg gggcggctgt cgcacaaacg gatagcgggt ttggtcggca
      121 ttgccccgca cccgagggag agcggggaaa ccaaattcaa aagccgctgc tttggcggaa
      181 ggtctgcggt gcgtaaggca ctgtatatgg ctaccgtggc agcgacacgt tttgaaccgc
      241 ttattcggga tttctaccaa cgcctgccgt ccgagggtaa gccgtataag gttgccgtta
      301 cggcatgtat gcgcaaactg ctgacgatat cgaatgcccg gatgcgtgat tattttgccg
      361 aaaacgatac cgccgaaaac gataccgccg aaaacggtat ccggacggct tgatttgagt
      421 tttggtattt ttgcccgacg gggtgaaaaa tacagttgct acggctcgcc gaatcatcag
      481 aaatacccga accgtcattc ccgcgcaggc gggaatccag accggtcggt gcggaaactt
      541 atcaggtaaa acggtttctt gagatttttc gtcttggatt cccactttcg tgtgaatgac
      601 gtggtgcagg tttccgtatg gatggattcg tcattcccgc gcaggcggga atctagtctg
      661 ttcggtttca gttattttcg ataaatgctt gttgcttttc atttctagat tcacactttc
      721 gcgggaatga cgaattttag gtttctgatt ttggttttct gtccttgtgg gaatgacggg
      781 atgtagattc gtaggaatga cgtggtgcag gtttccgtgc ggatggattc gt
//
LOCUS       DAWHXS010000209          812 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWHXS010000209 DAWHXS010000000
VERSION     DAWHXS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.209"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          205 a          165 c          220 g          222 t
ORIGIN      
        1 ttaaggcagc ggcaatattc aaagattatc tgaaagtccg agattctaga ttcccacgaa
       61 agtgggaatc caggatgtaa aatctcaaga aaccgtttta tccgataagt tcctgcactg
      121 acagacctag attcccgcct gcgcgggaat gacgggattt tagatttctg attttggttt
      181 tctgtttttg aggaatgacg ggatgcaggt tttcttaacc ctgcgtccta gattcccgct
      241 ttcgtggtaa tgacgggatg tgggttcgtg ggaatgacgc ggtgcaggtt tccgtgcgga
      301 tggattcgtc attcccgcgc aggcgggaat ttggaatttc aatgcctcaa gaatttatcg
      361 gaaaaaacca aaacccttcc gccgtcattc ccacgaaagt gggaatctag aaatgaaaag
      421 cagcaggaat ttatcggaaa tgaccgaaac tgaacggact ggattcccgc ttttgcggga
      481 atgacggttc agttgctacg gttattgtca ggtttcggtt atgttggaat ttcgggaaac
      541 ttatgaatcg tcattcccgc gcaggcggga atctagaccg gtcggtgcgg aaacttatcg
      601 ggaaaaaagg tttcttgaga ttttacgtcc tggattccca ctttcgtggg aatgacgtgg
      661 tgcaggttcg taggaatgac gtggtgcagg tttccgtgcg gatggattcg tcattcccgc
      721 gcaggcggga atccagacct tagaacaaca gcaatattca aagattatct gaaagtctga
      781 gattctagat tcccactttc gtgggaatga cg
//
LOCUS       DAWHXS010000210          789 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWHXS010000210 DAWHXS010000000
VERSION     DAWHXS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.210"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(272..>789)
                     /gene="rnhB"
                     /locus_tag="WIE22_002055"
     CDS             complement(272..>789)
                     /gene="rnhB"
                     /locus_tag="WIE22_002055"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ4128062.1"
                     /translation="AVILPEIFDLPGLTDSKKLSEKKRDALAEMIKNQAVAWHVAAAS
                     PEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDGNRIPEHLGIPAEAVVKGDSKIIE
                     ISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYGTKQHLEALKQYGVLPEHRRDFAP
                     VRNLLAQQTLF"
BASE COUNT          139 a          163 c          245 g          242 t
ORIGIN      
        1 gttttttgag tttcagtcat ttttgataaa ttcttgcagc tttgagtttc tagattcccg
       61 cttttgcggg aatggcggtt tggaagttac ctgaaattca aaaaaaaacg gaaaccggac
      121 ggattggatt cccgcctgcg cggggatgac ggattttagg ttttttttga ttttctattt
      181 ttcgcgggaa tggcggtttg ggttctttct ctttgaagtt gcgatgctgg aaatgccgtc
      241 tgaaggcttc agacggcatt tttgtgccgg tttaaaacag cgtctgctgc gcgagcaggt
      301 ttctgacggg ggcgaagtcg cggcggtgtt cgggcagtac gccgtattgt ttgagggctt
      361 ccagatgctg ctttgtgccg taacctttgt gtttgtcgaa accgtattgg ggatggcgtt
      421 gcgccagtgc gtacatttct gcatcgcgtg cggtctttgc caaaacggat gcggcggaga
      481 tttcgatgat tttgctgtcg cctttgacga ctgcttcggc agggatgcct aaatgttctg
      541 gaatgcggtt gccgtcgatg aatatttttt cgggacgcac agccaaaccg tcaacggcgc
      601 gtttcatggc gagcatggtg gcgtgcagga tgttgaggct ggcgatttct tcgggcgagg
      661 cggcggcaac gtgccacgca accgcctgat tttttatcat ttcggcaagc gcgtcgcgtt
      721 ttttctcgct gagttttttg gagtcggtca gtccgggcag gtcgaatatt tccggaagga
      781 tgacggcgg
//
LOCUS       DAWHXS010000211          769 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWHXS010000211 DAWHXS010000000
VERSION     DAWHXS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.211"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..764
                     /locus_tag="WIE22_002056"
     CDS             <1..764
                     /locus_tag="WIE22_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ4128063.1"
                     /translation="QPYKLDSQLIQHIDTLIRRKLSPEQVCAYLCKHHQITLHHSTIY
                     RYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVGIENRPAIVDQKSRIGD
                     WEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAEDTARAAVRALKAHKDRVHTIT
                     MDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLNENTNGLIRQYFPKQTDFRNISDR
                     EIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT          243 a          224 c          159 g          143 t
ORIGIN      
        1 gacaacccta taagctcgat tcgcagctga ttcagcacat cgacaccctt atccgccgca
       61 aactcagtcc cgaacaagta tgcgcctacc tgtgcaaaca ccaccagatc acgctccacc
      121 acagcaccat ttaccgctac cttcgccaag acaaaagcaa cggcagcacg ttgtggcaac
      181 atctcagaat atgcagcaaa ccctaccgca aacgctacgg cagcacatgg accagaggca
      241 aagtccccaa ccgtgtcggc atagaaaacc gacccgctat cgtcgaccag aaatcccgta
      301 tcggcgattg ggaagccgac accattgtcg gcaaaggaca gaaaagcgca ttattgacct
      361 tggtcgaacg cgttacccgc tacaccatca tctgcaaatt ggatagcctc aaagccgaag
      421 acactgcccg ggcagctgtt agggcattaa aggcacataa agacagggtg cacaccatta
      481 ccatggataa cggcaaagag ttctaccaac acaccaaaat aaccaaagca ttgaaagcgg
      541 agacttattt ttgtcgccct taccattctt gggagaaagg gctgaatgag aacaccaacg
      601 gactcatccg gcaatacttc cccaaacaaa ccgatttccg taacatcagt gatcgggaga
      661 tacgcagggt tcaagatgag ttgaaccacc gaccaagaaa aacacttggc tacgaaacgc
      721 caagtgtttt attcttgaat ctgttccaac cactaataca ctagtgttg
//
LOCUS       DAWHXS010000212          752 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWHXS010000212 DAWHXS010000000
VERSION     DAWHXS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.212"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..118)
                     /locus_tag="WIE22_002057"
                     /pseudo
     CDS             complement(1..118)
                     /locus_tag="WIE22_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(137..574)
                     /locus_tag="WIE22_002058"
                     /pseudo
     CDS             complement(137..574)
                     /locus_tag="WIE22_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          145 a          160 c          213 g          234 t
ORIGIN      
        1 taacgacgcc gtttttgact tcaacgcttt gaacatattt gcctttgatg tcggttgagg
       61 atgccacgcc ggcagaagtg ttgttgccgg gccattcgcc gtgattcagg taatactcgg
      121 gtgtttttgt gcaaacggca gatgatgcgt cgcggcaggt tgacggcagg tgcttggtgt
      181 cgattttgtc ggtgtcgttg ccggtggcgg cggtgacttc gtcgttgttg gcgccgttgg
      241 cgttggcgcg cgtaaccggc tgtccgcaga accattttac cgaaccgttt tgacgcttgg
      301 cccacaggga gagttctttg cctttgattt ctttgtttac gccggttgat ttcatttcgg
      361 cggtaacgac gccgtttgcg actataacgc tttgaacata tttgcctttg atgtcggagg
      421 cggaggatgc cacgccggca tcactgttgt tggcgggcca tatgccgtga ttcaggtaat
      481 actcggtaac ggctgatttt tgaccttcag ccaaaagaat gacgaatttt aggtttctgt
      541 ttttggtttt ctgtccttgt gggaatgatg aaattttaag ttttaggaat ttatcgaaaa
      601 aacagaaacc gctccgccgt cattcccgcg caggcgggaa tccaggtctg tcggtacgga
      661 aacttatcgg gaaaacggtt tctttagatt ttacgtccta gattcccact ttcgtgggaa
      721 tgacggttta ggtattttta tagaaagccg ta
//
LOCUS       DAWHXS010000213          724 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWHXS010000213 DAWHXS010000000
VERSION     DAWHXS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.213"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          222 a          216 c          126 g          160 t
ORIGIN      
        1 cccaccactt ttcgtcattc ccacgaaagt gggaatccag aatctcggac tttcagataa
       61 tctttgaata ttgctgttgt tctaaggtct agattcccgc ctgcgcggga atgacgaatc
      121 catccgtacg gaaacctgca ccgcgtcatt cccacgaacc tacatcccgt cattccctca
      181 aaaacagaaa accaaaatca gaaacctaaa atcccgtcat tcccgcataa ccgaaacctg
      241 acagtaaccg tagcaactga accgtcattc ccaccacttt tcgtcattcc cgcgaaagcg
      301 ggaatctaga atctcggact ttcagataat ctttgaatat tgccgctgcc ttaaggtcta
      361 gattccctcc tgcgcgggaa tgacgaatcc atccgtacgg aaacctgcac cgcgtcattc
      421 ccacgaaccc acatcccgtt attaccacga aagcgggaat ctaggacgca gggttaagaa
      481 aacctacatc ccgtcattcc ctcaaaaaca gaaaaccaaa atcagaaacc taaaatcaga
      541 aacctaaaat cccgtcattc ccgcacaagc gggaatccaa acttgtccgc acggaaactt
      601 atcggataaa acggtttctt tagattccac gttctagatt cccgcttgcg cgggaatgac
      661 gattcataag tttcccgaaa ttccaacata accgaaacct gacagtaacc gtagcaactg
      721 aacc
//
LOCUS       DAWHXS010000214          703 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWHXS010000214 DAWHXS010000000
VERSION     DAWHXS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.214"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            26..703
                     /locus_tag="WIE22_002059"
     CDS             26..703
                     /locus_tag="WIE22_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ4128064.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          217 a          136 c          156 g          194 t
ORIGIN      
        1 tcaaaaaacc tgataagctc ctaatatgaa actaaaaaat aagtatcaaa agttcagcaa
       61 aatttcagag caaaagttta gagaaataat ccgctgtttc gctcttgatt tgaccgcttc
      121 cgatactgct aaaatgaccg gcattagtgt acgcggtatc aatcctattt tcctgaaaat
      181 cagacacagg attgctgctc tgtgcgaaca aagctcacca ctgtccggtg tggtcgaatt
      241 agatgaatct tactttggtg cgcgacgtat caggggtaag cgcggtcgcg gagcgtcagg
      301 taaaaccatc gtatttggca tactgaaacg caatggcgtg gtctatacgg aaatcgttcc
      361 cgacgcttcg aaagcctcac taatcaaggt catacgtggt cacatatctg ctgacagtga
      421 aatcaatact gacggctgga aagcatatga cggtcttgtc gatatgggct atgagaagca
      481 ttaccgtgtc catcatggtt ccaatgagtt tgctcgagga aagcaacata tcaatggtat
      541 tgaatctttt tggagttata ctaaggggcg tttggctaaa tttcatggta tttccaaaga
      601 gatgttttat ctgcatctca aagaaacgga gtttaggttt aaccaccggc atgaagattt
      661 aggtaaaata ttaatgaaga tgcttcgaaa taacccaatt taa
//
LOCUS       DAWHXS010000215          698 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWHXS010000215 DAWHXS010000000
VERSION     DAWHXS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.215"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..698)
                     /locus_tag="WIE22_002060"
     CDS             complement(join(<1..479,479..698))
                     /locus_tag="WIE22_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ4128065.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDSQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARN"
BASE COUNT          161 a          161 c          136 g          240 t
ORIGIN      
        1 attacgagct aatttctcat agcgagtagc aatactacga aactgcttta atcgtgcaaa
       61 tgcattctcg actaagtggc gtaatttata aaggtagcta tcaaaatcag ggttaggttt
      121 cttggcattt ttacgctttg gtataatagc tttcataccg tgttctatcg ctttatctct
      181 gatttcttgc gagtcatacc ctttgtcagc gataaaatac tgggcttctt gtatgacttc
      241 tatcaagtcg tttgcaactt gactgtcgtg cacgttaccc ccagtgactt taaaatcgag
      301 cggatttcca tgcgagtcca cacataggtg tatttttgtc gtgtttccgc cacggctttg
      361 tccaattgct ctatcgaaac cacgccgagc tccacttgca tgttgatgac accgtacata
      421 acttccgtcg atgaataccc attctttgtc aatttctttt cgtagatcaa aaaaaaattc
      481 tgccacaagc cttttttaga ccatcggtta aagcggttat aagcggtttt ccatgaccct
      541 agctcaatag gtatgtctcg ccatggtgca cctgttctta gcttccatag tatggcttcc
      601 atcacggtac ggtcgttctt ccattgatga cagccgtgcg ctttcatggt tgtttgtaat
      661 tgttcccata tgttgtcagt tattgcggtt ctcgccat
//
LOCUS       DAWHXS010000216          675 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWHXS010000216 DAWHXS010000000
VERSION     DAWHXS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.216"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..81)
                     /locus_tag="WIE22_002061"
     CDS             complement(<1..81)
                     /locus_tag="WIE22_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ4128066.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAPFFCT"
     gene            complement(185..391)
                     /locus_tag="WIE22_002062"
     CDS             complement(185..391)
                     /locus_tag="WIE22_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128067.1"
                     /translation="MFDGAKVVRRMEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          179 a          178 c          112 g          206 t
ORIGIN      
        1 agtacaaaaa aacggagcag ttctttctgt acttttttct ttaatttgca gtgcgttatc
       61 ttcatatttc gagggtaaca tatctgctaa tctagtacag cccctagttt aaagctttgt
      121 ttcttaagtc cgcagagtat gccatggtta gaccttcaaa gttgagtatt gtactatttt
      181 gtttttaatt aactataaaa cgcttgtgtg aataggaaga ctgcagaata ttgctgcctt
      241 ttcctgtttg ctttaaataa acagaaaatg ccgtctgaaa ccgattttca gacggcattt
      301 gtcccttaaa acacgttttt tcaagcgcga ccataccaaa cacccacagg ttgccttcca
      361 tccttcgtac aacctttgct ccatcaaaca tccacttcct aaatactgat gtctgcttag
      421 gttgaatcgg cattcgtacg attacggcac accgcctaat ctgtacctgc gtctgttgcg
      481 cccaatacca aaggctacct gaaaatctcc caaacttttc aggtagcctt atcacgtttt
      541 cgactgtcac cacacgccca aagaccgcct gaaacccttt ttcagacgtt ccactccgat
      601 accccccaac aaaacgccgc ccgaacacat gttcagacgg catttgatgc ggctgcagac
      661 ttccccctat tttat
//
LOCUS       DAWHXS010000217          653 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWHXS010000217 DAWHXS010000000
VERSION     DAWHXS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.217"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WIE22_002063"
     CDS             complement(53..538)
                     /gene="fxsA"
                     /locus_tag="WIE22_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ4128068.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          194 a          212 c          116 g          131 t
ORIGIN      
        1 tttcaaaccg gaaaaatgcc gtctgaatct tcagacggca ttccattcat atttattcgt
       61 cttttttgtg ttcgatggcg tttcgggaac gattgccgcc gtaaggctct tcaaccgtat
      121 attccccctc gataatatcg tcatcgcggg aaaagccctc ttttctgccc gattggttca
      181 tgttgaaaaa attttccgca cctcctgcct gcaacactgc acctccctta aacggcagca
      241 gcagcaacac cgccaacacc gaggatacga atcccggact catcagacac accgccgcca
      301 ccgtataacg gataggccac aacatctgat aaacggatac cctcccgccg cttctcattg
      361 ccgcgcccgc caataaaaga ccggacagcc ccgtatgcct gagcatcagc acaccggcgg
      421 caaaacctgc cgccatcaaa aacaacgtcc agccgccgcc caaccaatcg gcaacccaca
      481 caatcgacat aatctccaaa aacagcagca ccaaaaaacc gataccgaaa aatctcattg
      541 accgtcatcc ttatatttaa gtaaacagca aaccgcccga acaggactcc aagcgacctg
      601 cctgtaaatg attacaaaac catgtgcttc aagccgaaac aatgtgaaat cac
//
LOCUS       DAWHXS010000218          647 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWHXS010000218 DAWHXS010000000
VERSION     DAWHXS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.218"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>647
                     /locus_tag="WIE22_002064"
     CDS             <1..>647
                     /locus_tag="WIE22_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HEZ4128069.1"
                     /translation="KTNGSIHYIKFKNNDDGLKQFRLWIKGNRIRKAYIGMEATGIYY
                     EKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSNLIADYIKRHQDTLIPYQI
                     PKNKALQKLINLKNQLQQHQKQIKNRLHSTEEDFIRNIHQDLIDTIQDKMEQVKIAIS
                     EQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKTANQFVSYAGLSPAII"
BASE COUNT          271 a          137 c          103 g          136 t
ORIGIN      
        1 aaaacaaacg gaagtatcca ttacattaaa tttaagaata atgatgatgg attaaaacag
       61 tttagattgt ggataaaggg aaacagaatc agaaaagcct atatcggcat ggaggcaaca
      121 ggcatctatt acgaaaaggc agcagatatg ctttcttcct actataccgt ttacgttatc
      181 aatcccttaa aaatcaagga ctacgggaaa agcaggttta accgtaccaa aaccgacaaa
      241 gcagattcaa acctgatagc agattacata aaaaggcatc aagatacatt gataccgtat
      301 cagataccca aaaacaaagc actgcaaaaa ctgattaacc ttaaaaatca attacagcaa
      361 catcagaagc aaattaaaaa ccgtcttcat agcactgaag aagacttcat aaggaacata
      421 catcaagact tgatagatac catacaggac aagatggaac aggtaaaaat agccatatcc
      481 gaacaaatca aaaaacaaac ggacaataac cattaccgca atcttcaaac catcccgagc
      541 ataggcaaag acaccgcatc agttctttat gcgcaactga cagaaaaaca ttttaaaacc
      601 gcaaaccagt ttgtatccta tgccggatta agtcccgcca tcataca
//
LOCUS       DAWHXS010000219          633 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWHXS010000219 DAWHXS010000000
VERSION     DAWHXS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.219"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            6..575
                     /locus_tag="WIE22_002065"
     CDS             6..575
                     /locus_tag="WIE22_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ4128070.1"
                     /translation="MLFPRIIAASAQRKLSVYLKKGGLVAYPTESCYGLGCLPTLAKA
                     LGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPAPKTFLLSAKSC
                     VLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRACRTEREVRRQFG
                     RDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT          170 a          131 c          170 g          162 t
ORIGIN      
        1 atattatgtt atttccgaga attatcgcag catcagcgca acgtaagtta agcgtttatt
       61 tgaaaaaagg tggtttggtc gcttatccga ccgaatcttg ttatggcttg gggtgtcttc
      121 cgacattagc caaagcactc ggtaagttgg cgcatttgaa aaaaagaccg caacacaagg
      181 gtatgattgt catcgggaat caatttgaac aattgcagcc tttacttcaa atgccgtctg
      241 aaaatcttca ggatatgctt agaaaagaat ggcccgcccc taaaacgttc ctattatccg
      301 ccaaatcctg tgtcttaccc gagttacgcg ggaaacagcg cagcaagttg gcggtcaggg
      361 ttcccgccca tgtaggtgcg cgccgcttgt gtcaggcttt gcaaacgcct ttggtctcga
      421 cttcgtgcaa ccgcgcaggc aaacgcgcgt gtcggacgga gagggaagtc agaaggcaat
      481 tcggacgcga tgtgtggatt gttggtggga gaatcggcag gcagaaatcg ccgagtcaga
      541 ttattgacgg ggaaacaggt aaacgtttgc gttagatatg ggtacttgaa tgaaaacttt
      601 tttgcataca caaagaaaaa attttggggc tgt
//
LOCUS       DAWHXS010000220          629 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWHXS010000220 DAWHXS010000000
VERSION     DAWHXS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.220"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            1..89
                     /locus_tag="WIE22_002066"
                     /pseudo
     CDS             1..89
                     /locus_tag="WIE22_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            192..353
                     /locus_tag="WIE22_002067"
                     /pseudo
     CDS             192..353
                     /locus_tag="WIE22_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          162 a          150 c          179 g          138 t
ORIGIN      
        1 tagacggcat tatgcgcaaa gcctgccgca accgtccgct gacggaaacg caaaccaaac
       61 gcaaccggta tttgtcgaag acccgttata gtggattaaa tttaaatcag gacaaggcga
      121 cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac tggtttaaat
      181 ttaatccact atatgtggtc gaacagagct tcggtacgct gcaccgtaaa ttccgctatg
      241 cgcgggcagc ctattttggt ctgctcaaag tgagtgcgca aagccatctg aaggcgatgt
      301 gtttgaacct gttgaaagcc gccaacaagc taagtgcgcc cgctgccgcc taaaaggaga
      361 ccggatgcct gattatcggg tatccgggga gggttaaggg ggtatttggg taaaattagg
      421 gggtatttgg gtaaaattag gaggtatttg gggcgaaaac agccgaaaac ctgtgttggg
      481 atttcggttg tcgtgaggga aaggaatttt gcaaaggtct caagctatgc cggctgctgc
      541 cgcccacttc atctgcgcca aatcctgccg cccaccgccg aagccctcat gcgttcgcgg
      601 tacagcgcgt atgtgctgca ccttatcga
//
LOCUS       DAWHXS010000221          620 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWHXS010000221 DAWHXS010000000
VERSION     DAWHXS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.221"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            153..542
                     /locus_tag="WIE22_002068"
                     /pseudo
     CDS             153..542
                     /locus_tag="WIE22_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          162 a          151 c          165 g          142 t
ORIGIN      
        1 aaatttttat agtggattaa caaaaaccag tacggcggtg cctcgcctta gctcaaagag
       61 aacgattctc taaggtgctg aagcaccaag tgaatgggtt ccgtactatt tgtactgtct
      121 gcggcttcgt cgccttgtcc tgatttttgt taatccacta tacctccgcc aatgcccatg
      181 agtgcaaaca cctgtcgccg ttgttggaag gactgcccaa aggtacgacc gtctatgccg
      241 acaaaggcta tgacagtgcg gaaaaccggc aacatctgga agaacatcag ttgcaggacg
      301 gcattatgcg caaagcctgc cgcaaccgtc cgctgacgga agcgcaaacc aaacgcaacc
      361 gatatttgtc gaagacccgt tatgtggtcg aacaaagctt cggtacgctg caccgcaaat
      421 tccgctatgc tcgggcagcc tatttcggac tgattaaagt gagtgcgcaa agccatctga
      481 aggcgatgtg tttgaacctg ttgaaagccg ccaacaggct aagtgcgccc gctgccgcct
      541 aaaaggcagc acggatgcct gattatcggg tatccgggga ggattaaggg ggtatttggg
      601 taaaattagg aggtatttgg
//
LOCUS       DAWHXS010000222          599 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWHXS010000222 DAWHXS010000000
VERSION     DAWHXS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.222"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..150
                     /locus_tag="WIE22_002069"
     CDS             <1..150
                     /locus_tag="WIE22_002069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128071.1"
                     /translation="SIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEACT
                     VKLQE"
     gene            172..465
                     /locus_tag="WIE22_002070"
     CDS             172..465
                     /locus_tag="WIE22_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128072.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          158 a          150 c          160 g          131 t
ORIGIN      
        1 tccattcggc agggaaagcc tgaagaagaa ttttcccgta agatggccgg ctgcctgacc
       61 gacttggaaa actgtttgga aaaatacctc gaacagttcg ggccggtatc ggaaagtatc
      121 gaagcctgta ccgtcaaact gcaggagtaa ccgtcgttct tcaacaggct gatgaaaacc
      181 aacgacaagc tcaataggca gattgacgtg ttgcagaaac agtcggcagc catccacaac
      241 gaagcctata tcgaaatgaa cacgctgctt taccgccacc gcgaagtggt ttccgtccac
      301 aaccgcaagg cggattatgc ggaaaagggc aaagagcgga ttgcgctgtt cccgcgcggc
      361 ttgaacggca tcaccaagct gcctgccgcc gtcctgttgc ccgagcgtcc cgattgtttt
      421 gatatgaagg cagtttggca aatgtttaat agaataccga ggtaggcgta tcggctgccg
      481 gataaaaatg ccgtctgaaa cggaagcgtt cagacggcat tgttgcagtg gaacaaatca
      541 aaatgcaccc cgtcattcct gtctgtacgg tgatagcggc agtgttttcc tgcttcgtt
//
LOCUS       DAWHXS010000223          594 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWHXS010000223 DAWHXS010000000
VERSION     DAWHXS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.223"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          192 a          135 c          128 g          139 t
ORIGIN      
        1 cccgcctgcg cgggaatgac gaatccatcc gcacggaaac ctgcaccacg tcattcctac
       61 gaacctacat cctgtcattc ccacaaggac agaaaaccaa aatcagaaac ctaaaattcg
      121 tcattcccgc gaaagtgtga atctagaaat gaaaagcaac aggcatttat cgaaaataac
      181 tgaaaccgaa cagactagat tcccgcctgc gcgggaatga cggctgcaga tgcccaacgg
      241 tctttatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacagata
      301 gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag
      361 gcgaggcaac gccgtactgg tttaaattta atccactata taaaaaattt ccagagaacc
      421 gatacaacag ttggaacttg ggtttgggaa tattacggta gatgaacttg gaacctctgt
      481 tatgctatgg tcttttatct caattgaaaa aagcgcgaat caaacggttc gcgctttttt
      541 cagacggtat tagggcgtgt cctcaattta atttagcatg aataatagag caag
//
LOCUS       DAWHXS010000224          582 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWHXS010000224 DAWHXS010000000
VERSION     DAWHXS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.224"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          167 a          140 c          151 g          124 t
ORIGIN      
        1 accgttttac tcgataaatt tccgtgccga ggggtctgga ttcccgcctt cgcgggaatg
       61 acggcatcag tttgcaggat tcggcgtgaa cggtaaaaac ggcagaatcg atgggatgcg
      121 gcaggctgaa gcccaccaaa acacaaaaat tccgatgccg tctgaaattt cgtcattccc
      181 gtgaaaacgg gaatccagcc ccgtgggagc agaaacttac accccgtcat tcccgcaaaa
      241 gcgggaatcc agtaaccgaa aaaccacagg aatctatcgg aaaaaaacag aaccccccgc
      301 cgccgtcatt cccgcgaacg cgggaatcta gaacgtagaa tctgagaaac cgttttactc
      361 gataaatttc cgtgccgagg ggtctggatt cccgcgttcg cgggaatgac ggcatatttt
      421 ttgcatttga tataaagggt cgtttgaatt ttgttcagca agtgcaaagt gttgcacata
      481 aaagggcgca ggatagaggc aaagcgggcg taggtcgggc tgtagcaact gtatttttca
      541 ccccgtcggg caaaaatata gtggattaac aaaaaccagt ac
//
LOCUS       DAWHXS010000225          553 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWHXS010000225 DAWHXS010000000
VERSION     DAWHXS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.225"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          158 a          144 c          116 g          135 t
ORIGIN      
        1 aaaaatcagg acaaggcgac aaagccgcag acagtacaaa tagtacgaaa ccgattcact
       61 tggtgcttca gcaccttaga gaatcgttct ctttgagcta aagcgaggca acgccgtact
      121 ggtttttgtt aatccactat accgtcgttc ccacggtcag gatttcagat tgcggacatc
      181 tgtcagaaaa gacaaaaaac cttccgccgt cattccctac aggcgagaat ccgatccgtt
      241 gaaattcggt tgttttaaat aaattcttgc agctttgatt ttctgttttt ccgataacgc
      301 cgtaactttg aaacgcgaaa gcggtaatcc gatccgttgg gattttgcaa cttcaaatca
      361 atccgcaaac tgaaatcccg tcattcccac gcagtcgtga atccgaacgc gtccgcacgg
      421 aaacctgcat cccgtcattc ccacgaaagt gggaatctag gacgtaaaat ctcaagaaac
      481 cgttttaccc gataagtttc cgcaccgaca gacctggatt cccgcctgcg cgggaatgac
      541 gaaatttcag cga
//
LOCUS       DAWHXS010000226          510 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWHXS010000226 DAWHXS010000000
VERSION     DAWHXS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.226"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..462
                     /locus_tag="WIE22_002071"
     CDS             <1..462
                     /locus_tag="WIE22_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ4128073.1"
                     /translation="ESNYSKKVTEFKHQNGTKKEDKTEHQGNGSFHAASSLGLSAIYD
                     FKLNDKFKPYIGARIAYGHVKHQIHSVESKTTTVTSNNGGPIPQGPTPKPPYHESNSI
                     SSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
BASE COUNT          154 a          151 c           96 g          109 t
ORIGIN      
        1 gaaagtaatt attctaaaaa agttactgaa tttaaacacc aaaacggcac caaaaaagaa
       61 gacaaaacag aacatcaagg aaacggcagc ttccacgccg cttcttctct cggcttatcc
      121 gccatttacg atttcaaact caacgataaa ttcaaaccct atatcggtgc gcgcatcgcc
      181 tacggacacg ttaaacatca gattcattca gtggaaagca aaaccacgac tgttacctct
      241 aacaacggag gtcctatccc acaaggtccg acccccaaac ctccctatca cgaaagcaac
      301 agcatcagca gcttgggtct tggtgtcatc gccggtgtcg gtttcgacat cacgcccaag
      361 ctgaccttgg acaccggata ccgttaccac aactggggac gcttggaaaa cacccgcttc
      421 aaaacccacg aagtctcatt gggcatgcgc taccacttct gattccccga taccgatgcc
      481 gtctgaacct tcagacggca tttttgatgc
//
LOCUS       DAWHXS010000227          502 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWHXS010000227 DAWHXS010000000
VERSION     DAWHXS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.227"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          120 a           96 c          136 g          150 t
ORIGIN      
        1 gaaagatttt caatgatgtt gcaggagcgg actatatcag gtttgtggcg atgtttcaac
       61 acaatatagc ggatgaacaa aaaagagaac gattctctaa ggtgctgaag caccaagtga
      121 atcggttccg tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
      181 tccgctataa agaccgtcgg gcatctgcag ccgtcattcc cgcgcaggcg ggaatgacgg
      241 gattttaggt ttctgttttt ggttttctgt ccttgtggga atgatgggat gtaggttcgt
      301 aggaatgacg tggtgcaggt ttccgtacgg atggattcgt cattcccgcg caggcgggaa
      361 tccagacctt agaacaacag caatattcaa agattatctg aaagtccgag attctagatt
      421 cccactttcg tgggaatgac ggttcagttg ctacggttac tgtcaggttt cggttatgtt
      481 ggaatttcgg gaaacttatg aa
//
LOCUS       DAWHXS010000228          492 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWHXS010000228 DAWHXS010000000
VERSION     DAWHXS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.228"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          125 a          144 c          116 g          107 t
ORIGIN      
        1 ggaatccaga cctccgacgc ggcgggaatc tatcggaaat gactgaaacc ccgagattct
       61 agattcccac tttcgtggga atgacgtggt gcaggtttcc gtatggatgg attcgtcatt
      121 cccgacaaca ccgtaatctc gaaattcgtc attcccgcgc aggcgggaat ccagccccct
      181 gacgcggcgg gaatctatcg gaaatgactg aaaccccgag attctagatt cccacttccg
      241 tgggaatgac ggttcagttg cgttccgaca acaccgcaat cttgaaaccc gtccgacaac
      301 accgtaatct taagacccgt cattcccgca caggcgggaa tccagacctg tccgcacaga
      361 aacttatcgg ataaaaacag ttgcccaaac cccgcgttct agattcccac tttcgtggga
      421 atgacggttc agttgcgttc cgacaacacc gtaatcttga aattcgtcat tcccgcacag
      481 gcgggaatct ag
//
LOCUS       DAWHXS010000229          486 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWHXS010000229 DAWHXS010000000
VERSION     DAWHXS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.229"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            32..394
                     /locus_tag="WIE22_002072"
     CDS             32..394
                     /locus_tag="WIE22_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ4128074.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
BASE COUNT          149 a          141 c          104 g           92 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaagcgaaca ggaccggcgc gaacggttgc tgtcgctgac
      301 cgaaaaaggc aaagcccatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatt
      421 ttccatgagc accttcttcc ggcaaaccgc ccaagccatg attgccaaac acatcgaccg
      481 cttccc
//
LOCUS       DAWHXS010000230          480 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWHXS010000230 DAWHXS010000000
VERSION     DAWHXS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.230"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(297..372)
                     /locus_tag="WIE22_002073"
     tRNA            complement(297..372)
                     /locus_tag="WIE22_002073"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(336..338),aa:Glu,seq:ttc)
BASE COUNT          129 a          114 c          121 g          116 t
ORIGIN      
        1 gtctgaaaat tcagaagttc tagattcccg ttttcacggg aatgacgaag ggttgtggga
       61 atgacgaagt ttcagacggc atcaaatgct ttgtcaggct tcagacagca tcgtccaccc
      121 tctgccgtca ttcccgcgca ggcgggaatc cagccttgtc ggcacggaaa cttatcggat
      181 aaaacggttt cttcaaccct acgctctaga ttcccgtttt cacgggaatg acgaagagtg
      241 tgagaatgac ggttcataaa taaaaaaacc tgcttaaaac aagcaggttt ttgaattggc
      301 acgcccacgg ggattcgaac cccggttgcc gccgtgaaag ggcagtgtcc taggcctcta
      361 gacgatgggc gcgtgggaat gggcattata catacttcta gaaatttagc aatacgatgc
      421 cgtctgaaac ttcgtcattc ccgcgaaagc gggaatctag aatctccaag ttttcagaca
//
LOCUS       DAWHXS010000231          479 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWHXS010000231 DAWHXS010000000
VERSION     DAWHXS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.231"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(<1..235)
                     /locus_tag="WIE22_002074"
     CDS             complement(<1..235)
                     /locus_tag="WIE22_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128075.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGG"
     gene            complement(219..>479)
                     /locus_tag="WIE22_002075"
     CDS             complement(219..>479)
                     /locus_tag="WIE22_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ4128076.1"
                     /translation="SKYFGQSMAVIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDI
                     WLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          146 a           96 c           75 g          162 t
ORIGIN      
        1 tgccgccccc cgccaatacg ccggcgcggg aaatgctggc ggttattttg gcgttgattc
       61 gggcttttgc gcccgtaggg atgtgtgtta aatctaccgt ttttattaaa tcagatgaat
      121 aagttttact atttttaggt acaaacttat gaattttcgc accttgtccg gtatcaactg
      181 aaacagtttc agatattttt actgcatttg cattcgcttc aaacgaatac atcatcaaaa
      241 ttgcaattat cgacaatttc gcaaaattca aatttgtata ttttatgacc atctttcagg
      301 gattctttaa ttaccatttc tgaattatca gaaaatgaga ttagccaaat atcatgttta
      361 attcttctat tccagaaaaa agagaaacaa tcaataacat tttcagactt attaatcttc
      421 gcaaattcaa caaattcaga ttgcgctata accgccatcg attgcccaaa atacttgct
//
LOCUS       DAWHXS010000232          455 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWHXS010000232 DAWHXS010000000
VERSION     DAWHXS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.232"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          122 a          110 c          110 g          113 t
ORIGIN      
        1 atctatccgt acggaaacct gcaccacgtc attcccacga aagcgggaat ccagtttttt
       61 gagtttcagt catttccgat aaattgcctt agcattgaat gtctagattc ccgcctgcgc
      121 gggaatgacg aatccatccg tacggaaacc tgcactacgt cattcccacg aaagagggaa
      181 tccagttttt tgagtttcag tcatttccga taaattgcct tagcgttgaa tgtctaaatt
      241 cccgcctgcg cgggaatgac ggattttagg ttgggggcat ttattgggaa aagcagaaac
      301 cgctccgccg tcattcccac gaaagtggga atctagaaat ttaatgttgc ggcactagcc
      361 aaaaaaaccg aaaccgaacg gactagattc ccgcctgcgc gggaatgacg ggatttgaga
      421 ttgcggcatt tatcgggagc aacagaagcc gctct
//
LOCUS       DAWHXS010000233          443 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWHXS010000233 DAWHXS010000000
VERSION     DAWHXS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.233"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          121 a           91 c          105 g          126 t
ORIGIN      
        1 ggattcccac tttcgtggga atgacggcgg agcggtttct gctttttcca ataaatgccc
       61 ccaacctaaa atccgtcatt cccgcgtagg cgggaatcta gacattcaat gctaaggcaa
      121 tttatcggaa atgactgaaa ctcaaaaaac tggattccca ctttcgtggg aatgacggga
      181 tgcaggtttc cgtatggatg gattcgtcat tcccgcgcag gcgggaatct aggtctgtca
      241 gtgcggaaac ttatcaggta aaacggtttc ttgagatttt gcgtcctgga ttcccacttt
      301 cgtgggaatg acgcgattag agtttcaaaa tttattctaa atagctgaaa ctcaatgaac
      361 cggattcccg cctgagcggg aatgacggga ttaagttttc aaaattcatc agaaattatt
      421 gattcaatgg cataaatttt tga
//
LOCUS       DAWHXS010000234          441 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWHXS010000234 DAWHXS010000000
VERSION     DAWHXS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.234"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          137 a          111 c           82 g          111 t
ORIGIN      
        1 aatccagtgc gttgagtttc agctatttag aataaatttt gaaactctaa tcgcgtcatt
       61 cccacgaaag tgggaattca gaacgtaaaa tctcaagaaa ccgttttacc cgataagttt
      121 ctgtgccgac agacctagat tcccgcctgc gcgggaatga cgggatttga gattgcggca
      181 tttatcggga gcaacagaat ccgctctgcc gtcattccca cgaaagtggg aatccaggac
      241 gcaaaatctc aagaaaccgt tttgcccgat aagtttccgc accgacagac ctagattccc
      301 gcctgcgtgg gaatgacggc ggaaagattc tatttttccc gataatcacc cacaatctca
      361 aattccttca ttctctcaaa aacaaaatca gaaccctaaa tcccatcatc cccatctatg
      421 tgaatataaa aattttaaaa a
//
LOCUS       DAWHXS010000235          438 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWHXS010000235 DAWHXS010000000
VERSION     DAWHXS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.235"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          110 a          138 c           93 g           97 t
ORIGIN      
        1 ttttacacaa ttcccaccgt ttcccatcat tcccgataca ccgtaatctc gaaacccgtc
       61 attcccgcgc aggcgggaat ccagacctgt ccgcacagaa acttatcgga taaaacggtt
      121 tctttagatt ttacgtccta gattcccact tccgtgggaa tgacggttca gttgcattcc
      181 cgacaacaac gcaatctcga aacccgtcat tcctgcgcag gcgggaatcc agacctccga
      241 cgcggcggga atctatcgga aatgactgaa accccgagat tctagattcc cacttccgtg
      301 ggaatgacga ttcagttgcg ttccgacaac accgcaatct cgaaacccgt cattcccgcg
      361 caggcgggaa tccagccccc tgacgcggcg ggaatctatc ggaaatgact gaaaccccga
      421 gattctagat tcccactt
//
LOCUS       DAWHXS010000236          421 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWHXS010000236 DAWHXS010000000
VERSION     DAWHXS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.236"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            complement(1..107)
                     /locus_tag="WIE22_002076"
                     /pseudo
     CDS             complement(1..107)
                     /locus_tag="WIE22_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT           96 a           86 c          111 g          128 t
ORIGIN      
        1 gcggtggatg ccacgccggc agaattgttg ttggcgggcc atatgccgtg attcaggtaa
       61 tactcggtaa cggctgattt ttgaccttcg gctaaaagga tggattcgtc attcccgcaa
      121 aagcgggaat ccagccttgt cggtacggaa acttatcggg aaaacggttt ctttagattt
      181 tacgttctag attcccgctt ttgcgggaat gacgaatttt aggtttctgt ttttggtttt
      241 ctgtccttgt gggaatgatg aaattttaag ttttaggaat ttatcggaaa aaacagaaac
      301 cgctccgccg tcattcccgc gcaggcggga atccagcccc atcggtacgg aaacttatca
      361 ggtaaaacgg tttctttaga ttttgcgttc tggattccca ctttcgtggg aatgacggga
      421 t
//
LOCUS       DAWHXS010000237          408 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWHXS010000237 DAWHXS010000000
VERSION     DAWHXS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.237"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            125..>408
                     /locus_tag="WIE22_002077"
     CDS             125..>408
                     /locus_tag="WIE22_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128077.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLID"
BASE COUNT          138 a           74 c           82 g          114 t
ORIGIN      
        1 atccggataa aaggcttttc gatccacatc acgatgtaga aagtgagagg caatatcagc
       61 tttatctcga cagaatgtat gatcttcatg caaatcaaaa ttaacctaaa aaggaaaaat
      121 taatatgttt aatcaaactc aaactgtaac ttatcccgca acttttttag gagctaaaaa
      181 attcaaaggc gaaattgatg gctctaatat cgacacttgt tccgtattgg ttgcaacacc
      241 tttgccggca cagtcgggaa atgctgttgg attcacggca gcacaaatga agttcgggga
      301 cagtaagaat ttctcaaaat tagagaatct caaatacccg tgcgaagtta tggtaacggt
      361 tgaaatgact tcgacaggta agggcatggt tccttcatta attgattt
//
LOCUS       DAWHXS010000238          408 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWHXS010000238 DAWHXS010000000
VERSION     DAWHXS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.238"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            125..>408
                     /locus_tag="WIE22_002078"
     CDS             125..>408
                     /locus_tag="WIE22_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128078.1"
                     /translation="MFNQSQTVTYPATLLGAKKFKGEIDGSDIDTCSVLVASPLPTQS
                     GNAVGFTAAQMKFGDSSNFARLENLKYPCEVMLTVEMTSTGKGMVPSLID"
BASE COUNT          130 a           71 c           86 g          121 t
ORIGIN      
        1 atccggataa aaggcttttc gatccacatc acgatgtaga aagtgagagg caatttcagc
       61 tttatctcaa cagaatgtat gattttcatg caaatcaaat ttaatttaaa aaggaaaaat
      121 gaatatgttt aatcaaagtc aaactgtaac ttatcctgca acattattgg gtgcgaagaa
      181 atttaaaggc gaaattgatg gctctgacat cgacacttgt tctgtattgg ttgcttcacc
      241 attgccgact caatcgggca atgcggtggg ttttaccgca gcgcaaatga aattcggcga
      301 tagcagtaat tttgcacgat tggaaaatct caaatacccg tgcgaagtga tgttgacagt
      361 cgaaatgact tcgactggta aaggcatggt tccttcatta attgattt
//
LOCUS       DAWHXS010000239          405 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWHXS010000239 DAWHXS010000000
VERSION     DAWHXS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.239"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           96 a           91 c           94 g          124 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttag ctcaaagaga acgattctct
       61 aaggtgctga agcaccaagt gaatcggttc cgtactatct gtactgtctg aggcttcgtc
      121 gccttgtcct gatttttgtt aatccactat aaagaccgtc gggcatctgc agccgtcatt
      181 cccgcgcagg cgggaatcta gtctgttcgg tttcagttat tttcgataaa tgcctgttgc
      241 ttttcatttc tagattcccg cttttgcggg aatgacggtt cagttgctac ggctactgtc
      301 aggtttcggt tatgttggaa tttcgggaaa cttatgaatc gtcattcccg cgcaggcggg
      361 aatctagact ttagaacaac agcaatattc aaagattatc tgaaa
//
LOCUS       DAWHXS010000240          404 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWHXS010000240 DAWHXS010000000
VERSION     DAWHXS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.240"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>404
                     /locus_tag="WIE22_002079"
     CDS             <1..>404
                     /locus_tag="WIE22_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ4128079.1"
                     /translation="YSVNTKEVLRNNNGNRKELKTENQENGTFHAVSSLGLSAIYDFK
                     LNDKFDKFKPYIGARVAYGHVKHQVHSVKKETTTTFLAPTQGATVPGKIVEGPTPKPP
                     YHESHSISSLGLGVIAGVGFDITPKLTLDTGYR"
BASE COUNT          127 a          112 c           84 g           81 t
ORIGIN      
        1 tattccgtca acacaaaaga ggtgctaaga aacaataatg gcaacaggaa agaactgaag
       61 acggaaaatc aggaaaacgg tacattccac gcagtctcat cgctcggctt atccgccatt
      121 tacgatttca aactcaacga taaattcgat aaattcaaac cctatatcgg tgcgcgcgtc
      181 gcctacggac acgttaaaca tcaggttcat tcagtgaaaa aagaaaccac gactactttc
      241 cttgcaccaa cgcaaggcgc tacagtgcca ggcaagatcg tagaaggtcc gacccccaaa
      301 cctccctatc acgaaagcca cagcatcagc agcttgggtc ttggtgtcat cgccggtgtc
      361 ggtttcgaca tcacgcccaa gctgacttta gacaccggat accg
//
LOCUS       DAWHXS010000241          401 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWHXS010000241 DAWHXS010000000
VERSION     DAWHXS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.241"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            141..>401
                     /locus_tag="WIE22_002080"
     CDS             141..>401
                     /locus_tag="WIE22_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4128080.1"
                     /translation="MLSSGVNKEIQGKKLSLWAKRQNGSVKWFCGQPVTRDKADKADD
                     GVTAANDKQIDTKHLPSTCRDDSSTGCIETPRADFKHFQKISR"
BASE COUNT          120 a          104 c          100 g           77 t
ORIGIN      
        1 gcggtttgct gaaagaaggt gctcatgaga aatcccctaa atgtcttggt gggaatttag
       61 gggattttgg ggaattttgc aaaggtctcg gaaaatatgt taaggaagtt acagtcgcaa
      121 acggcgtcgt taccgccaca atgctttcaa gcggcgtaaa caaagaaatc caaggcaaaa
      181 aactctccct gtgggccaag cgtcaaaacg gttcggtaaa atggttctgc ggacagccgg
      241 ttacgcgcga caaagccgac aaagccgacg acggcgttac cgccgccaac gacaagcaga
      301 tcgacaccaa gcacctgccg tcaacctgcc gcgatgattc atctaccggt tgcatagaaa
      361 caccacgcgc cgacttcaaa cacttccaaa aaatcagccg t
//
LOCUS       DAWHXS010000242          395 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWHXS010000242 DAWHXS010000000
VERSION     DAWHXS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.242"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          118 a           99 c           83 g           95 t
ORIGIN      
        1 ttttaggttg ggggcattta ttgggaaaat cagaaaccgc tccgccgtca ttcccacgaa
       61 agtgggaatc tagaaattta atgttgcggc actagccaaa aaaaccgaaa ccgaacggac
      121 tggattcccg cctgcgcggg aatgacgaat ccatccatac ggaaacctgc accacgtcat
      181 tcccacgaaa gtgggaatct agttttttga gtttcagtca ttcccgataa attgccttag
      241 tattgaatgt ctagattccc gcctgcgcgg gaatgacggc ggagcggttt ctgttttttc
      301 cggtaaatac ccacaagcta aaatcccgtt attttcacaa aaacagaaaa ccaaaaacag
      361 aaacctgaaa ttcgtcattc ccacgaacct gcacc
//
LOCUS       DAWHXS010000243          389 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWHXS010000243 DAWHXS010000000
VERSION     DAWHXS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.243"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            77..342
                     /locus_tag="WIE22_002081"
                     /pseudo
     CDS             77..342
                     /locus_tag="WIE22_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           96 a           98 c          116 g           79 t
ORIGIN      
        1 gggtggcggc aatgcggact tttctattgg atacgattga agtaatgtcg gattcgagaa
       61 tccgacctac ggaaaactga aagagcatcg gctgcaggac ggcattatgc gcaaagcctg
      121 ccgcaaccgt ccgctgtcgg aggcgcaaac caaacgcaac cggtatttgt cgaagacccg
      181 ttatgtggtc gaacagagct tcggtacgct gcaccgtaaa ttccgctacg ctcgggcagc
      241 ctatttcgga ctgattaagt gagtgcgcaa agccacctga aggcgatgtg tttgaacctg
      301 ttgaaagccg ccaacaggct aagtgcgccc gctgccgcct aaaaggcagc ccggatgcct
      361 gattatcggg tatccgggga ggattaagg
//
LOCUS       DAWHXS010000244          368 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWHXS010000244 DAWHXS010000000
VERSION     DAWHXS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.244"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            205..>368
                     /locus_tag="WIE22_002082"
     CDS             205..>368
                     /locus_tag="WIE22_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128081.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAA"
BASE COUNT          109 a           72 c           85 g          102 t
ORIGIN      
        1 ctttcacaaa gcccgcaaca aaggaaaaat atcatgaaaa tgaaccttgc aacactaatt
       61 atcggctggg tggtctgtat gtttcttttt cttttcgcca tcctctattt tatcggctaa
      121 aaacgagatt cgggaaagac ttcgcccgga tgaagcaagt caagaagtcg tcttacttta
      181 aacatcaaaa aaaggaaaaa aacgatgaac atcgttaaaa aatatgctgt aaaagcattg
      241 ttggcagccg gtatcttcac accggctatt gttatggcag atggctttga tgcatccgcg
      301 attggtacgc aagtggcgaa tgtaatcatg ggttttgtgg caatggtttc cgccgtgggt
      361 atggcggc
//
LOCUS       DAWHXS010000245          363 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWHXS010000245 DAWHXS010000000
VERSION     DAWHXS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.245"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>363
                     /locus_tag="WIE22_002083"
     CDS             <1..>363
                     /locus_tag="WIE22_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595-like element IS1016C family transposase"
                     /protein_id="HEZ4128082.1"
                     /translation="DEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRVY
                     TVVVDNAKSETLLPVIKKKIMPDSIVYTNSLSSCDKLDVSGFIHYRINHSKEFADRQN
                     HINGIENFWNQAKRVLRK"
BASE COUNT           99 a           81 c          100 g           83 t
ORIGIN      
        1 ccgatgaggt ttttgagggc cctgtcgagc cggacgaaag cgatttcggc ggacggcgta
       61 aaggcagacg tggtcgcggt gcggcaggaa aagtggttgt cttcggcatt ctgaaacgca
      121 acggacgggt ctataccgtt gtggtggata atgccaagtc tgaaacgtta ctccctgtca
      181 tcaaaaagaa aatcatgccg gacagcattg tttataccaa tagtctgagc agctgcgaca
      241 agttggacgt gagcggtttt atccattacc gcatcaacca ttccaaggag tttgcagacc
      301 gtcaaaacca cattaacggc attgagaact tttggaatca ggcaaaacgc gtcttgcgaa
      361 aat
//
LOCUS       DAWHXS010000246          359 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWHXS010000246 DAWHXS010000000
VERSION     DAWHXS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.246"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          131 a           89 c           76 g           63 t
ORIGIN      
        1 tccaaaaatc caaaatcaca ggaagttatc aaaaaaacag aaaccatccg ccgtcgttcc
       61 cgcgaaagcg ggaatccgga accggaaacc acaaaaattt atcgggaaaa ccgaaactca
      121 aaaacaacgg aaaccgaacg gacaggattc ccgcctgcgc gggaatgacg gctcatacat
      181 cacccaaaaa attgaaacca aacagatggg attccctctt gcgtgaggct gacagatgcc
      241 gtctgaaaga ctttcagacg gcatagcttt ttctctttga atttatagtg gattaacaaa
      301 aatcagaaca ggcgacgaag ccgcagacag tacaaatagt acggaaccga ttcactcgg
//
LOCUS       DAWHXS010000247          357 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWHXS010000247 DAWHXS010000000
VERSION     DAWHXS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.247"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            194..>357
                     /locus_tag="WIE22_002084"
     CDS             194..>357
                     /locus_tag="WIE22_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128083.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDASAIGTQVANVIMGF
                     VSMVSAVGMAA"
BASE COUNT          113 a           71 c           69 g          104 t
ORIGIN      
        1 ctttcacaaa gcccgcaaaa aggattaaaa atgaataaag caactttaat tatcggattt
       61 tttgtttgcc tcattctttt cattttctca attctctatt tcataaatta aaaacaaaat
      121 tcgggagcag gcttcgcccg gatttaaacc taagaagccg tattacttta aatatcaaaa
      181 aaggaaaaaa acgatgaaca tcgttaaaaa atacgctgta aaagcagcct tggcagccgg
      241 tatcttcaca ccggccattg ttatggcaga tacctttgat gcatccgcga ttggtacgca
      301 agtagcgaat gtaatcatgg gtttcgtgtc aatggtttcc gccgtgggta tggcggc
//
LOCUS       DAWHXS010000248          349 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWHXS010000248 DAWHXS010000000
VERSION     DAWHXS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.248"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           73 a           75 c           86 g          115 t
ORIGIN      
        1 ggatatttat agtggattaa caaaaaccgg tacggcgttg tctcgcctta gctcaaagag
       61 aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactgtt tgtactgtct
      121 gtggctcgcc gccttgtcct gatttttgtt aatctgctat aaagaccgtc gggcatctgc
      181 agccgtcatt cccgcgcagg cgggaatcta ggtctgttcg gttccagtta tttccgataa
      241 atgcctgttg cttttcattt ctagattccc actttcgtgg gaatgacggt tcagttacta
      301 cggttactgt caggtttcgg ttatgttgga atttcgggaa acttatgat
//
LOCUS       DAWHXS010000249          334 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWHXS010000249 DAWHXS010000000
VERSION     DAWHXS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.249"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           96 a           73 c           71 g           94 t
ORIGIN      
        1 tttttttgaa tttcgggcaa cgcttgaatc ttcattccgc gcaggcggaa attatcggtg
       61 cggtacggca acttttttcg atatgaaaag accgtcattc ctgtaaaaac aaaaaatcaa
      121 aaacagaaaa ttgaaattcg tcattcccgc gcaggcggga atctaggacg taaaatctat
      181 agtggattaa caaaaaccag tacagcgttg ccttgactta gctcgaagag aacgattctc
      241 taaggtgctg aagcaccgag tgagtcggtt ccgcactatc cgtactgtct gcggcttcgt
      301 cgctttctcc tgatttttgt taatccacta taaa
//
LOCUS       DAWHXS010000250          333 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWHXS010000250 DAWHXS010000000
VERSION     DAWHXS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.250"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT          111 a           66 c           87 g           69 t
ORIGIN      
        1 gccgcgttgg gcggaaagtc tcagcaaaat cttatgtatg ataattggca ggagcgcgga
       61 aaaccgtttg aaggaatcgg cgggggcgtg gtcggatcgg caaactgaag aaaacggcaa
      121 gagagaaaaa agacccgtaa accgtttgaa tatagacggc ttacgggtct ttgtttcgcg
      181 caaagcaagg gctaaggcag tcaggcagca aatcccgcaa tgtattaaaa cagacgcgta
      241 gaaatgccgg ctgcctttat ccatcctcaa aattgaatat catcctagcc gtatcaaggc
      301 tgtataaata aggaaaatac caatgaatat aat
//
LOCUS       DAWHXS010000251          314 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWHXS010000251 DAWHXS010000000
VERSION     DAWHXS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.251"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           75 a           71 c           86 g           82 t
ORIGIN      
        1 agtccgcttg ttcggcaaat gagagggcgg attgcgcgct tgtcagataa accacgtgtt
       61 taaacgggcg gcaatgaggt acgcgcagag ccttgaagcg cagtcgatat attattttca
      121 ccaaaacgga cgaccccgcc tgccttgcaa acccttaaaa tgaagccacc cggattaatc
      181 cgagtggctg tggaaaatca cttaccgctt gatttattta aaatttatgg tataatttac
      241 cttagctggc agatgccgca tcgcggcagg ttgacggtag gtgcttggtg tcgatcttgc
      301 cgttggtgtc ggtg
//
LOCUS       DAWHXS010000252          308 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWHXS010000252 DAWHXS010000000
VERSION     DAWHXS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.252"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            15..293
                     /locus_tag="WIE22_002085"
     CDS             15..293
                     /locus_tag="WIE22_002085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128084.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETETFAKFLSLPTAETQTQVFSCFQLFSPQIPP"
BASE COUNT          101 a           60 c           64 g           83 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc
      241 aaacacaggt tttcagctgt tttcagctgt tttcgcccca aataccccct taatcctgcc
      301 cggatacc
//
LOCUS       DAWHXS010000253          304 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWHXS010000253 DAWHXS010000000
VERSION     DAWHXS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.253"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            84..>304
                     /locus_tag="WIE22_002086"
     CDS             84..>304
                     /locus_tag="WIE22_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEZ4128085.1"
                     /translation="MLRLTALTLCTVLALGACSPKDSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTL"
BASE COUNT           66 a           98 c           65 g           75 t
ORIGIN      
        1 attatcattt atccttttct aaagccgttc cggtttgtcc gaccggcggc tttgccccaa
       61 tatccccatt ttggagacac cctatgttgc gtttgaccgc attgaccctc tgcaccgtcc
      121 tcgccttggg cgcgtgttcg ccgaaagatt ccgactctgc cccacaagcc aaagaacagg
      181 cagtttccgc cgcacaatcc gaaagcgcgt ccgttaccgt caaaaccgcg cgcggcgatg
      241 ttcaaatacc gcaaaacccc gaacgtatcg ccgtttacga tttgggtatg ctcgacactt
      301 taaa
//
LOCUS       DAWHXS010000254          302 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWHXS010000254 DAWHXS010000000
VERSION     DAWHXS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.254"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           84 a           91 c           54 g           73 t
ORIGIN      
        1 aaccgtcatt cccgccactt ttcgtcattc ccgctcaggc gagaatctag aatctcggac
       61 tttcagataa tctttgaata ttgccgctgc cttaaggtct ggattcccgc ctgcgcggga
      121 atgacgaatc catccgcacg gaaacctata tcccgtcatt cctacgaacc tacatcccgt
      181 cattccctca aaaacagaaa accaaaatta gaaatctaaa atcccgtcat tcccgcgcag
      241 gcgggaatcc aaacttgtcc gcacggaaac ttatcggata aaacggtttc ttagattcca
      301 cg
//
LOCUS       DAWHXS010000255          298 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWHXS010000255 DAWHXS010000000
VERSION     DAWHXS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.255"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           77 a           49 c           61 g          111 t
ORIGIN      
        1 tgaaaggggc ttttttatgt cagcatcaaa tgtaatattt tcctgttcgt actggagaat
       61 atttaaaaaa tcagattatt gcgttttatg tttctatcag ttcagacatg gcgaaccgca
      121 taaactcatt aatcaagaga atttttcaaa gctttatcag gcgttcgatt atatagattc
      181 ggttggttcg aattttccgg taattatcac aaaagacggt tgtggtcttt cttattgatc
      241 tttaacagtt tgtcaggatt tggctttcgg tcgttgaccg tcgtacgcgc tttagcgc
//
LOCUS       DAWHXS010000256          283 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWHXS010000256 DAWHXS010000000
VERSION     DAWHXS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.256"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           73 a           65 c           62 g           83 t
ORIGIN      
        1 gacaaattcc taaaacttaa aattccgtta ttcccgcgag aacagaaaac caaaaataga
       61 aacctaaaat ctcgtcattc ccgcgcaggc gggaatccag tccgttcggt ttcgcttgtt
      121 ttgggtttcg ggtaacttcc acttcgtcat tcccgcgaaa gtgggaatcc ggttcgttga
      181 gtttcagtca tttccgataa atcaccgcaa cattaaattt ctagattccc gcttttgcgg
      241 gaatgatggc ggagagggtt tgttgtttcg gataaatccc tgt
//
LOCUS       DAWHXS010000257          258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWHXS010000257 DAWHXS010000000
VERSION     DAWHXS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.257"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           68 a           63 c           72 g           55 t
ORIGIN      
        1 tgactgaaac tcaaaaaact agattcccac tttcgtggga atgacgaagg agtgagaatg
       61 gcgaaggggc gggaataacg gagtgcaggt ttccgtgcgg atggattcgt cattcccaca
      121 cagacaggaa tccggatttg cccacgcgga aacttatgcg ccgtcgttcc cgcgaaagcg
      181 ggaatccaga cccttcggca cagaaactta tcggataaag ggtttcttga gattctacgt
      241 cctagattcc cgcctgcg
//
LOCUS       DAWHXS010000258          257 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWHXS010000258 DAWHXS010000000
VERSION     DAWHXS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.258"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           87 a           54 c           52 g           64 t
ORIGIN      
        1 tttttgttaa tccactataa tttattgaag atggctgaaa ttcaaagtta cagaaattta
       61 taaacaaaaa acagcaaccc gccgccgtca ttcccgcgaa agcgggaatc cagtcccttg
      121 accttaagat aatctttaat tcgtcattcc cgcaaaagcg agaatccgga agcgaaaagt
      181 ggtgggaatg tattagaaat gactgaaact caaaaaaccg gattcccgcc tgcgcgggaa
      241 tgacgattga tacgttt
//
LOCUS       DAWHXS010000259          239 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWHXS010000259 DAWHXS010000000
VERSION     DAWHXS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.259"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            36..>239
                     /locus_tag="WIE22_002087"
     CDS             36..>239
                     /locus_tag="WIE22_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ4128086.1"
                     /translation="MLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRL
                     PTFLCNDVVKNPQVEKYLADYQAH"
BASE COUNT           66 a           51 c           60 g           62 t
ORIGIN      
        1 acggcacagg cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc
       61 cgattgaggc gtttacccgc gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt
      121 atatgcactt ccacaaagcc aacgagtttt tgggtatgac ccgcctgccg acattcttat
      181 gtaacgatgt ggttaaaaat ccgcaagtgg aaaaatactt ggcagattat caggcacac
//
LOCUS       DAWHXS010000260          234 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWHXS010000260 DAWHXS010000000
VERSION     DAWHXS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.260"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            <1..>234
                     /locus_tag="WIE22_002088"
     CDS             <1..>234
                     /locus_tag="WIE22_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128087.1"
                     /translation="QQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVK
                     KMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGE"
BASE COUNT           85 a           38 c           45 g           66 t
ORIGIN      
        1 cagcaaaaca aagcacactt tatccaattt ctacgcgacg gtttggatag cgtggacgat
       61 attgttatcc gaaaagatgc gtgtagttta agcacgacta tgggagaaag attgcttact
      121 tacggggtta aaaaaatgcc atctgcctat cctgaatatg aagcttatga agataaaaga
      181 catattcctg aaaatccata ttttcatgaa ttttactata ttaaaaaagg agaa
//
LOCUS       DAWHXS010000261          221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWHXS010000261 DAWHXS010000000
VERSION     DAWHXS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.261"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            10..210
                     /locus_tag="WIE22_002089"
     CDS             10..210
                     /locus_tag="WIE22_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ4128088.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
BASE COUNT           76 a           31 c           48 g           66 t
ORIGIN      
        1 ggttgattta tgaaatttga agaacgtttc atagttcaag acttggaaac gcatgacttt
       61 atttatcccg atcctttcgg tgatgtgggg tttactcaaa atattaaatc agcaggtcaa
      121 tttgaaagct acgaagatgc gttgaattca ggcataaatg aaataggcgg aggattccag
      181 atatttcagt tcttcgtaaa atcggaataa aagaaaaaca g
//
LOCUS       DAWHXS010000262          214 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWHXS010000262 DAWHXS010000000
VERSION     DAWHXS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.262"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
BASE COUNT           60 a           69 c           49 g           36 t
ORIGIN      
        1 tgaattttcc gcagccgcaa taagccctct ccctagccct ctcccacagg agagggaaca
       61 aagtgccgcc gccgtttcag acggcctgaa aaccgaaagc agcctgtatt ccatcgcaaa
      121 taatctgcac ggacaaatcc gacagcaacc taccccctcc cccgtggggg aagattgggg
      181 agagagcaaa acagttgccg ctcaaaccga attt
//
LOCUS       DAWHXS010000263          205 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM02517
            SAMN17286474-rid13901693.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWHXS010000263 DAWHXS010000000
VERSION     DAWHXS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286474
            Sequence Read Archive: SRR13407203
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001242982.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001242982.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:27:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,090
            CDSs (total)                      :: 2,034
            Genes (coding)                    :: 1,926
            CDSs (with protein)               :: 1,926
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 47 of 108
            Pseudo Genes (incomplete)         :: 60 of 108
            Pseudo Genes (internal stop)      :: 15 of 108
            Pseudo Genes (multiple problems)  :: 14 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286474-rid13901693.denovo.263"
                     /strain="NM02517"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Maryland"
                     /lat_lon="39.0458 N 76.6413 W"
                     /collection_date="2006"
                     /collected_by="Maryland Active Bacterial Core
                     surveillance"
     gene            78..>205
                     /locus_tag="WIE22_002090"
     CDS             78..>205
                     /locus_tag="WIE22_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ4128089.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQSVTVANGVITAQMASSNVN"
BASE COUNT           53 a           51 c           50 g           51 t
ORIGIN      
        1 aggtctgtcg gtacggaaac ttatcgggta aaacggtttc ttgagatttt gcgttctgga
       61 ttcccacttt cgtgggaatg acgggattta atgatgccgc cggcgtggca tcctccccct
      121 ccgacatcaa aggcaaatat gttcaaagcg ttacagtcgc aaacggcgtc attaccgccc
      181 aaatggcttc aagcaacgta aacaa
//
